LOCUS       DAWKOD010000001        54801 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000001 DAWKOD010000000
VERSION     DAWKOD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 54801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.001"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1005
                     /locus_tag="WJK73_000001"
     CDS             52..1005
                     /locus_tag="WJK73_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ7556553.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WJK73_000002"
     CDS             complement(1116..1439)
                     /locus_tag="WJK73_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556554.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WJK73_000003"
     CDS             complement(1612..2973)
                     /locus_tag="WJK73_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ7556555.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WJK73_000004"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WJK73_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ7556556.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5752)
                     /locus_tag="WJK73_000005"
     CDS             complement(5039..5752)
                     /locus_tag="WJK73_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556557.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTFMLWGMNDPFSQTTDRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5799..6311)
                     /locus_tag="WJK73_000006"
     CDS             complement(5799..6311)
                     /locus_tag="WJK73_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ7556558.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6458..7789
                     /gene="pmbA"
                     /locus_tag="WJK73_000007"
     CDS             6458..7789
                     /gene="pmbA"
                     /locus_tag="WJK73_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ7556559.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8086..8289)
                     /locus_tag="WJK73_000008"
     CDS             complement(8086..8289)
                     /locus_tag="WJK73_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ7556560.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8532..8834
                     /gene="clpS"
                     /locus_tag="WJK73_000009"
     CDS             8532..8834
                     /gene="clpS"
                     /locus_tag="WJK73_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ7556561.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8836..11115
                     /gene="clpA"
                     /locus_tag="WJK73_000010"
     CDS             8836..11115
                     /gene="clpA"
                     /locus_tag="WJK73_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ7556562.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11162..13033)
                     /locus_tag="WJK73_000011"
     CDS             complement(11162..13033)
                     /locus_tag="WJK73_000011"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ7556563.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(12994..13365)
                     /locus_tag="WJK73_000012"
                     /pseudo
     CDS             complement(12994..13365)
                     /locus_tag="WJK73_000012"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13384..14259)
                     /locus_tag="WJK73_000013"
     CDS             complement(13384..14259)
                     /locus_tag="WJK73_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7556564.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14338..15558)
                     /locus_tag="WJK73_000014"
     CDS             complement(14338..15558)
                     /locus_tag="WJK73_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ7556565.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGIADGYKGRLKDVVWKTLGEVF
                     DLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNSLKHISKSAVKGGKLFPAKS
                     IMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLI
                     NTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTLTHSISEGLPHEIALRRKQY
                     EYYREQLLAFPKAT"
     gene            complement(15724..16734)
                     /locus_tag="WJK73_000015"
     CDS             complement(15724..16734)
                     /locus_tag="WJK73_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ7556566.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16731..18275)
                     /locus_tag="WJK73_000016"
     CDS             complement(16731..18275)
                     /locus_tag="WJK73_000016"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ7556567.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSIVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WJK73_000017"
     CDS             complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WJK73_000017"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ7556568.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19393..20511)
                     /locus_tag="WJK73_000018"
     CDS             complement(19393..20511)
                     /locus_tag="WJK73_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7556569.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WJK73_000019"
     CDS             complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WJK73_000019"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7556570.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WJK73_000020"
     CDS             complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WJK73_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ7556571.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WJK73_000021"
     CDS             complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WJK73_000021"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ7556572.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WJK73_000022"
     CDS             complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WJK73_000022"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ7556573.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24939..25778
                     /gene="htpX"
                     /locus_tag="WJK73_000023"
     CDS             24939..25778
                     /gene="htpX"
                     /locus_tag="WJK73_000023"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ7556574.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSQGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26079..26639)
                     /locus_tag="WJK73_000024"
     CDS             complement(26079..26639)
                     /locus_tag="WJK73_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556575.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(26845..27237)
                     /locus_tag="WJK73_000025"
     CDS             complement(26845..27237)
                     /locus_tag="WJK73_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7556576.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(27354..27764)
                     /locus_tag="WJK73_000026"
     CDS             complement(27354..27764)
                     /locus_tag="WJK73_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7556577.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(27778..28215)
                     /locus_tag="WJK73_000027"
     CDS             complement(27778..28215)
                     /locus_tag="WJK73_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7556578.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(28627..29925)
                     /locus_tag="WJK73_000028"
     CDS             complement(28627..29925)
                     /locus_tag="WJK73_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ7556579.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30028..31179)
                     /locus_tag="WJK73_000029"
     CDS             complement(30028..31179)
                     /locus_tag="WJK73_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7556580.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            31514..32893
                     /gene="norM"
                     /locus_tag="WJK73_000030"
     CDS             31514..32893
                     /gene="norM"
                     /locus_tag="WJK73_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ7556581.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVLWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            33072..34112
                     /gene="murB"
                     /locus_tag="WJK73_000031"
     CDS             33072..34112
                     /gene="murB"
                     /locus_tag="WJK73_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ7556582.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            34124..34774
                     /locus_tag="WJK73_000032"
     CDS             34124..34774
                     /locus_tag="WJK73_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ7556583.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            34829..35605
                     /locus_tag="WJK73_000033"
     CDS             34829..35605
                     /locus_tag="WJK73_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7556584.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            35656..36222
                     /locus_tag="WJK73_000034"
     CDS             35656..36222
                     /locus_tag="WJK73_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ7556585.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            36242..37132
                     /locus_tag="WJK73_000035"
     CDS             36242..37132
                     /locus_tag="WJK73_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ7556586.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            37157..37915
                     /locus_tag="WJK73_000036"
     CDS             37157..37915
                     /locus_tag="WJK73_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7556587.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(37970..38635)
                     /locus_tag="WJK73_000037"
     CDS             complement(37970..38635)
                     /locus_tag="WJK73_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7556588.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(38835..39731)
                     /locus_tag="WJK73_000038"
     CDS             complement(38835..39731)
                     /locus_tag="WJK73_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ7556589.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            39800..40957
                     /locus_tag="WJK73_000039"
     CDS             39800..40957
                     /locus_tag="WJK73_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ7556590.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(41020..42021)
                     /gene="hemB"
                     /locus_tag="WJK73_000040"
     CDS             complement(41020..42021)
                     /gene="hemB"
                     /locus_tag="WJK73_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ7556591.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(42204..42488)
                     /gene="yhbY"
                     /locus_tag="WJK73_000041"
     CDS             complement(42204..42488)
                     /gene="yhbY"
                     /locus_tag="WJK73_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ7556592.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            42598..43218
                     /locus_tag="WJK73_000042"
     CDS             42598..43218
                     /locus_tag="WJK73_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ7556593.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGGWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            43282..45249
                     /gene="ftsH"
                     /locus_tag="WJK73_000043"
     CDS             43282..45249
                     /gene="ftsH"
                     /locus_tag="WJK73_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ7556594.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            45471..45902
                     /locus_tag="WJK73_000044"
     CDS             45471..45902
                     /locus_tag="WJK73_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ7556595.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            45895..46173
                     /locus_tag="WJK73_000045"
     CDS             45895..46173
                     /locus_tag="WJK73_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ7556596.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            46226..46804
                     /gene="pth"
                     /locus_tag="WJK73_000046"
     CDS             46226..46804
                     /gene="pth"
                     /locus_tag="WJK73_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ7556597.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSAEHR
                     QQIDDAIAKSLQAIPDIISGKWEEATRFLHSK"
     gene            46864..47070
                     /locus_tag="WJK73_000047"
     CDS             46864..47070
                     /locus_tag="WJK73_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ7556598.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            47067..47429
                     /locus_tag="WJK73_000048"
                     /pseudo
     CDS             47067..47429
                     /locus_tag="WJK73_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            47660..49075
                     /locus_tag="WJK73_000049"
     CDS             47660..49075
                     /locus_tag="WJK73_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ7556599.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            49194..49703
                     /locus_tag="WJK73_000050"
     CDS             49194..49703
                     /locus_tag="WJK73_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7556600.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            49866..51248
                     /locus_tag="WJK73_000051"
     CDS             49866..51248
                     /locus_tag="WJK73_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ7556601.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(51268..52023)
                     /locus_tag="WJK73_000052"
     CDS             complement(51268..52023)
                     /locus_tag="WJK73_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7556602.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSTKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(52033..52749)
                     /locus_tag="WJK73_000053"
     CDS             complement(52033..52749)
                     /locus_tag="WJK73_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7556603.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(52739..53566)
                     /locus_tag="WJK73_000054"
     CDS             complement(52739..53566)
                     /locus_tag="WJK73_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7556604.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            53851..54759
                     /locus_tag="WJK73_000055"
     CDS             53851..54759
                     /locus_tag="WJK73_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ7556605.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13008 a        15071 c        13505 g        13217 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcttt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgccacat cgaaatcctt
     2761 gggttcgatg ccgagcagca gatccctgat cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc agacggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcgggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggccgtctg aaacgcggga
     3661 atgttgtgtt cgagtatgct caagcccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaagtgaag attgggaatg
     3781 gaacgccccg aaccgcgcac ttcaccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcaacg atgccgacca cgccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt caggaaaagc attcagcgcg
     3961 tcttgcagca tagactgctc gatttcgcga cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaaggtcgcc cagattcggc
     4321 tctttgatgc cgtctgaaaa ataatcgcgg cggcgcaatt cggcacgcaa cgccgtcaac
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccaca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtatcggcag gcaaatggtg gtcggttaca
     4501 atgacatcca aacccaattc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tcaaccgtaa tcagcaaatc cacgccttgc gcggcggcga tttcggccag ctcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acgaggaaat ccactttcgc gcccatcgcc
     4681 gccaaaccgt ccaaaccgac ggcacacgcc gtcgcgccgt cggcatcata gtcggcgaca
     4741 atcaggattt tttcctgacg ctcaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcagcg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaggcaca aagccgggcg attaaaggat cggcgccggc ggtgagcaaa
     4921 tgattgaaaa cgtcggtatt gacggatcgg gtttggattt tgacggacat agcgtggaac
     4981 tttacagtgc ggatgtgttt gaaatgccgt ctgaaggctt ttcagacggc attgccgttc
     5041 atttgtctga ggaattcgtg gctgcaatca acgccgcgac aggcaaggcc agcaccgcgc
     5101 ccaccgcatc cagtatcaac gtggtgggcg tatatgcaat attttcaaac aacttggatt
     5161 tgtcggtatg ttttttcgta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 gataatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gagacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagttcga ccgcttcaaa ctcaagctgt ttcagcttgg
     5341 cgatgtcggc aggtctgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctggcggg cgattcgagt ttgaccggca gggcttggta ggcaaagcgc gggttgggct
     5461 caaccatttg aaactgcttg tccaacccgg ccttcaaaat gcccgtcagc ttcgccgaat
     5521 cttcaggatt gacgacgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
     5581 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtccttg tcaacgtgtt
     5641 tgcggtcggt cgtttggctg aacgggtcgt tcatacccca caacataaac gtacagccat
     5701 tcagcatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatcct
     5761 ccaatctaaa taacggcgga tgtcgtctga aaatatgcct aaatttccgc gtccccgttt
     5821 tccataacgg ttttcaattc ttgaaacagg gcgcggaaat ttttcggcgg tttgttttgc
     5881 tcctgctctt ttttggtgtt gcggatcaag gtacgcaatt taccggcgtc cgcatgcgga
     5941 aaatccgaca taaactgcgt caacgcgccg tcgtctgcca acagccgtac gcgcgcctgt
     6001 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaagaaac
     6061 gcctcgatgg gcgcgggatc cgtgtcgcgc atcagcctgc cgataaattg cgcctgccgc
     6121 ttgagcgcgc cgttggatgt gattttttta taggcggtta ccgcctcgta caaatccgca
     6181 tccaaaccga tttttttcag cgtatcgttt gagagcttgg tcaacgccat acccaaatcc
     6241 tgcaaatcgt tcatctgctt tttcatttgg gttttgctga tccattcgtc ttcttgttca
     6301 aacatcttga ttctcgaatt tttccaatag cgcattttaa cagaatgccg ccgcgttcag
     6361 acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt tgcccctgcc gcccgaaacg
     6421 gttaaaatgc cgcaaagcat catcaagaaa gatttctatg ctgttcaacc acaccgcttc
     6481 cgaactcctc gacctctgcc gccgcacgct cgacttggca aaagcagcgg gcgcgaccgc
     6541 cgccgaagcc gatttcagcg aatccatcgg acaaagcgtc agcgtgcggc tgggcgaaat
     6601 cgaacaaatc gagttccagc aggacaagtc gctggacatt accgtttacg tcggcaaacg
     6661 caaaggccgc gctggcaccg ccgatttctc cgaacaagcc ctgcgcgaca ccgtccgcgc
     6721 cgccgccgac atcgcccgcc acaccgccga agacggttgc gccggacttg ccgaccccga
     6781 gttgatggcg caacacatcg gcgaccccga cctttaccac gaatgggatt tggatacgga
     6841 agccgccgtc ggcttggcaa aacaatgcga acaagccgcc ctgaacgagg atgagcgcat
     6901 cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac caatacgttt acggcaacac
     6961 ccacggtttt gccgcacacc ggcaaggcac gcaccacagc atttcctgca gcgtcgttgc
     7021 cgccgacgaa aacgggatgc agcgcgacta ctggtacgat tccgcctgcc gccatccgga
     7081 tatggacagc cccgaaacca tcggtcaaac cgccgcccgc cgcactttgc gccgactcgg
     7141 cagccgcagc atcccgacag gcagctaccc cgtcctcttc gataccacca tttcgggcgg
     7201 tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg ctctaccgcc aaagcagttt
     7261 cctgatcgac agcatcggca aaaaagtcct gcccgatttc ctcaacctgc gcgaagaacc
     7321 gcacatcccc cgctctttcc gcagcagcta ttttgatgcg gaaggcgttg ccaccgcacc
     7381 gcgcttcgtg attcaaaacg gcattgtcga aggctatttc ctcagcagtt acagcgcgcg
     7441 caaactcggt atgcagacca cgggcaacgc cggcggcgcg cacaacctgt atttgaacca
     7501 tacgcacgaa acgcaatccg acctgctgaa agaaatgggg acgggattat tggttaccga
     7561 actgatggga cagggcgcga acaccattac cggtgactac tcgcgcggcg cggcgggttt
     7621 ttgggtggaa aacggcgtga ttgcctaccc cgtccacgag attaccgtag ccggacgctt
     7681 gcaggatatg taccgcgaca ttgtcggcgt ggcggatgac gctttgcggc gttcgtccaa
     7741 caaaatcggc tcgattctga ttgcggggat gacggttgcc ggaagctgaa cgccctgccg
     7801 atgccgtctg aagacgaagg cagcacgttt tgccccgtgc ctacccgttt gccgttttat
     7861 gccgcaccgc cttttgccga caacattgca cattttttca tcagcactga ttttccctta
     7921 tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca ggataaggcg
     7981 tgcggggatt ggggtttttc cgccgtcggc aaaacggcag acaaaaacgg cgggacattg
     8041 cctgtcccgc cgttttcaaa tatacatttg accgtacatc aggaattaag cagcctgaat
     8101 gttggcggcc tgtttgcctt tagggccggt ggttacgtcg aaagagacgc gttggccttc
     8161 tttcagggtt ttgaaacctt ccatattgat tgctgagaag tgagcgaaca aatcttcgcc
     8221 gccttcatca ggagtgatga aaccaaaacc tttagcgtcg ttaaaccatt ttacgatacc
     8281 ggttgccatt agaaacttcc tatactcaaa aattaacaaa atcagcaaaa caaggcatac
     8341 ggcaaaccga gccgtctggc gttccttcca gcttgatata ctcggcggat gcccaatcct
     8401 gcttaaccgt ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg
     8461 ggaaaatagg caaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa
     8521 tgggacacac tatgaccgcg caacaccaat ccgacacgct cttgcaccgt ctgaacaccc
     8581 tgccgccgaa acgttacggc gttttcctat tgaacgacga ttacaccacg atggaatttg
     8641 tcgtcgaaat cctgaccgaa atctttatgc tcggacaaga acaggcggta gcggtgatgc
     8701 tcttggttca tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat attgcccaaa
     8761 ccaaacaaca acaagtcatg cagcgggcaa aagccgaagg gcatccgctg caatgtattg
     8821 tcgaggagat ttaatatgct tgcacccgaa ttggaacaga ttttgcagca gctttaccgc
     8881 gaggcgcgta aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc
     8941 gaagaagatg cctccgtgcc gaacgtctta aaactatgcg gcgcggattt gaaagtggtg
     9001 tccgaacagc tcgccgccag cgttgccgaa aacacccccc tgattcccga acacctttta
     9061 gacacggtcg aaacccggcc cacgctcggc ttccaacgcg tgatgcaacg ggcgatggtg
     9121 catacccagt ctgccggaaa agccgcagtc gaaccgttgg acgttttggt cgcgctgatg
     9181 agcgaaaccg acagccacac cgtctatttc ctcaagctgc aatcggttac gcgttttgaa
     9241 gttttgcgct gtattgccca tggctcgccc gatgacgagg atggcaatga ttcagacggc
     9301 ctgggacgcg agggcgaaga agcggaacaa aaaaccggct ccctttccga ctacaccgtc
     9361 aacctcaaca ccgaagtcaa agccggccgt atcgaccctt tgattggtcg caaacacgaa
     9421 atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct tttggtcggc
     9481 gaagcaggcg tgggcaaaac cgcgctggcg gaaggtttgg cacatcaaat cgtcaacggc
     9541 ggcattccag acgcgcttaa agatgccgaa gtgtacgcgc tggatatggg ctcgctgttg
     9601 gcgggcacga aataccgtgg cgactttgaa gcgcgggtca aatccgtctt gaaacagctc
     9661 gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat cggcgcaggc
     9721 agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct ggcaaaaggt
     9781 tcgctgcgct gcatcggcgc aaccacctac gacgaatacc gcaccatttt cgacaaagac
     9841 tatgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt ttccgaaacc
     9901 gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt ccgctatacg
     9961 caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga gcgtttcctg
    10021 cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat tctgcccaaa
    10081 tccaaacaga aaaaagtcat cggcaaagcg caaatcgaaa ccgtcatcgc caaagtcgcg
    10141 cggattccag aaaaaaccgt gtcgcacgac gacaaacagg tgctgcaatt cctcggccgc
    10201 gatttgaaaa acatggttta cggtcaagaa aacgccatcg acgcgctggt tgccgccgtc
    10261 aaaatgtcgc gttcgggctt gggactgccc gacaaaccga taggcagctt cctcttctcc
    10321 ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc ttgcctactc gatgggcgta
    10381 ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc gcgcctcatc
    10441 ggcgcgcctc cgggctacgt cggcttcgaa caaggcggcc ttttgaccga agccgtcaac
    10501 aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc cgacattttc
    10561 aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg caagagtgcc
    10621 gatttccgca acgtcatcct gattatgacc accaacgcgg gcgcggaaag cctcagccgc
    10681 cccggcctcg gctttaccgc caagcgcgaa cgcggcgacg aaatgcaggc catcaacaaa
    10741 ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccgtttgc acccctgtcc
    10801 gaacccatta tcgccaaagt cgtggacaaa ttcctgctcc ggctcgaaca ccggctcctc
    10861 gacaagaaag tcgaagccga attcacaccg gcattgcgca aatatctggc gagaaaaggt
    10921 ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga ttcaggaaaa aatccgcaaa
    10981 ccgctcgccg acgaactcct gttcggcaaa ctatccaacg gcggcttcgt gcggatagac
    11041 tgggatgcgg caaaagaaga agccgtgttg aaatttaaga aaagcaaggt taaacctgaa
    11101 acagaaactg tttaaccgat tgaaaaataa aatgccgtct gaaatttcag acggcatttt
    11161 tttaaatatc tgtccctatt ccggcaaact tttccacaaa cgccgcaatc ttttgaaaaa
    11221 cactttgttt cttcgtcaga tattgcgggt tgagcgggct cattttcggc agggtttcgg
    11281 tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc aaataacgtt
    11341 tggcggcggt ttcgttcagg tcttcggcgg caatcagctc tgctgcttcg cgcctcatca
    11401 cctcttgagc gtaggtgtaa aaggtttcca gtatggcagg aacgtcgggg actttgtcca
    11461 aatccgtatc gttgatgaaa tccacaatca ggctctcttt ggcgcggtgg ccgatgctgg
    11521 cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt tttttgtggt
    11581 gctcgaaaac cagttgcaag atgtaatcca gattgatttc ctgcgatttg agcaaatcca
    11641 cctcaaaaac cacatcgtcc caatcgattt ttgattgttc tttctgttcg cctgcttttt
    11701 ggcggcgcag ccagtcgcgg atgtcgttat aggcagaacg gtagtcctgc actgcccttt
    11761 cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcagg tagtattttt
    11821 cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac tcgcgcagcg
    11881 cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg ccgaagagtt
    11941 tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg aaacgctcgc
    12001 gcaattcttt tgccacatcc aaataaccgc gccgcacttc gcccgtccga tcatcgatat
    12061 agccgttcat gtattcttcg tagctttttt ccaacaccac gtttttggtg tttttatcgc
    12121 cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag acaatattgc
    12181 cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc atcaggccgt
    12241 gatagcgcag gtttttatcg acaaacagcg tgttcagcgt cggcgcgtca aaacccgtca
    12301 aaaacatacc gaccacaatc agcaaatcca cttcctggtt tttcacccgt tttgccaaat
    12361 cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg aaacaggcgt
    12421 tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc gcttccggtt
    12481 caaaggtctc atcaacaatt tcaccgacgg cgttctgctc ttcgttggcc gcaaaggaaa
    12541 aaatggtggc cactttcagc gggtgcaggc tgcctgcctg ttgtgttttg aacgcttcgt
    12601 aatagcactt cgccgcatcc acgctgctga ccgcaaacat ggcgttgaag cctttaccgc
    12661 ccgcattcag gcggtgcgtt ttctgcctga actggttcag gatatattgc gtgatttcgc
    12721 ggatgcgttc ggggtgcagc agggcttggc ggttttcggc agcactcagt ttcttctcat
    12781 cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc actttgaatt
    12841 tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcaac tcacgcccga
    12901 acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc gtaccggtaa
    12961 aaccgaactg gcagaatttc ttaaatttct ttttcaggtt ttttgcgctt cgccgaattg
    13021 cgagcggtgg cattcgtcga aaataaatac aacctgctga tggtaaaccg gcagattatc
    13081 ttcactcttc atcaggttgt tcagcttttg gatggtggta acgatgattt tgttgtcgtc
    13141 tttttccaaa ttgcgtttca agcccgccgt gctttccgaa ccattcacgc tgtcgggcga
    13201 aaaacgttgg tattccttca tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa
    13261 aaccttgtcg ataaacgccg attccgtcgc cagacgcgcc gccttaaagc tggtcagcgt
    13321 tttgccgctg cccgtggtat gccaaacata gccgccgctt tccggtttgc tccaattctt
    13381 tacctacgcc gagctgttga ttttccacaa aatgcgttcg gcggcggcaa tctgatacgg
    13441 ccgcataatc agcagcgtat cattcgcatc gaacacgctg taatgcagca atacgcccag
    13501 caatacgctt ttctgcagga acgtggcggt aaagtctttc aaatccttaa tcggatgatt
    13561 gtccgaccgc gcccaattca tcgtgaaatc aaagctgttt ttgtcgcgct tggtggtgtt
    13621 ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag atttgcagga atttgaacag
    13681 cgaattttcg ctgttgaagc tctctttgct gtaacggtgc acctgattga atgcttcgcg
    13741 caccgccacg ccgcgctttt tcaattcaat ctgcaccagc ggcaggccgt ttaccagcac
    13801 ggtaacgtca tagcggtttg catacgtgcc cgtctgctca aactgattga taatctgcac
    13861 atggttgcgg gcaaggtttt tcttgtccag cagataaatg tttttcagac ggccgtcatc
    13921 aaaagcgaaa tcataaatat ggtcgtcatg gattttgcgg gttttatcgg taatgttttc
    13981 agacggcctg tccagatatt ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac
    14041 atcgttcagc cgctgcaact gcgcgcgcag gttttccagc agcctgcttt ggctgttcaa
    14101 atccttgcgg tattcgtaac cctgattctg caaatcggcg attaactccg cttccaactg
    14161 gttttccgat tggtagctgc ccgactgttc gattttttca tattggtcga gcacgatgaa
    14221 attcggcgtt tcggcgatgg gtttggtttc gaggttcatg ggtcgtcctt tgcagtatcg
    14281 ggttttaaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc tgctttttca
    14341 ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag
    14401 ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc agggtatcga atttgtctag
    14461 gatggcggca attttttcct gttcggggag tggtgggatg gggattttta atttctttaa
    14521 ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc tctgctccgc
    14581 aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa attcatcttt
    14641 aattgtaaaa ttggtcagtt gttggttact aatataatta acttttatta acgcatgctc
    14701 tcctatggtt gccgttgtcg acatcattat tgatttagca ggaaacagct tgccgccctt
    14761 aactgcgctt tttgatatat gcttcaatga attatctaaa atacggctat tttccctaat
    14821 atcttccatt ctaaaccaag ggattgagcc attttcccaa tattctttat ttgatttgga
    14881 aggggtatag ccattcttca agtcaaacac ctcccccaac gtcttccaaa ccacatcttt
    14941 cagacggcct ttatagccat cagctatccc ccccccgatt tgattgtcaa aatctaaaag
    15001 aaagtcgcgg taataccggt attggcgttt gcgcagggct aattcagctt ccagctcggt
    15061 gaatttgtca agtattttta caattttttg ttgggtttcc aggggtggga tagggattaa
    15121 taattttttc aagttactgg catttagagc aggaatacct gtaaatgtac ataaggcatg
    15181 aatctcttgt tcaaattcaa gtaatatatg atagagaaaa cgaacatcca attctgttct
    15241 attcttaata gtaactgcat aatttaagtt gccacgaaaa tatcttcctt cttgccacgt
    15301 tattgagccg actcccgctc ctcttgttgt gatgccgata gggtcatttt cagtgttcca
    15361 ttcatcaata tagccaagcg gctctttgcc actgttaata actggataac taccaatatt
    15421 atttgagatt ttctgcttac ttacagcttg tcctgttttt atgatagcta tcccattttc
    15481 tccacccaac ggcttccact ccaccggtgc cgtctgaatc atctcaatca attttttcgc
    15541 tttgctttgc atatccatgg ttgttttccg tttctttccc gctgtggcgg gatgtggggc
    15601 ggttgtgggg tgcttcggct cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc
    15661 gtcctgcttt caggctgcct gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg
    15721 tagttatttt gtgcggtctt ttttctgttt gatctgttgc acggctgttt tttccaacgc
    15781 tttaacgctt tccagatagg cttgctccac cggcgacagg atgcgcgctt cataggcgcg
    15841 gtattcgcgt tcggctttct gctctgcctg tttgcggctg atgctgcctg caccctgtaa
    15901 tgtgccttgt ccgtacagcc cggaaaattt gtccaattcg gctatccagt cgcgcatata
    15961 catggggctt tgctcctgcg ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg
    16021 gttcaggcgg aacagttcgt cttcggtcag atagtttttg gcgattttgg cttcattcag
    16081 cgtggggatt gcgccttgaa aggtggtcag ccccataaag tctttgctgc tgtcggcacg
    16141 gctgtatatc agctcagctg cggtttgccg gctggcggca tagtgcagtt tgttttgaac
    16201 ggcggcaaaa aaggtttggc tttcgctgct tttggggttg tagtcttggc tggtggcata
    16261 taaatcaagc acttgccggt atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt
    16321 gagcagttct ttccaatagt cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc
    16381 aaagcctttg gtcagatatt cgtccagccg ttcggttgcc cattggcgga attggatgcc
    16441 ccgcgcggaa cggacgcggt agccgacggc aataatcatg ggcagggaat aatgggcgat
    16501 tttgcggttt accttgcgcc cgttttcatt ttgaactgtc aactggaagt tgacagttgc
    16561 cttctcttcc aattcttgct ctgcaagaat ggttttaatg tgtttgctga tattttgttt
    16621 ggtggtttgg tacagttctg ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc
    16681 ctgcaaggca aattgggcag tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca
    16741 atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt cgattttggc aacggtttcg
    16801 ctgatttcgg cgttaagctg tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca
    16861 tagctgctga cggcgaggtt gtagccgttg tctttgacgg tttgctgggc ggcgttttgg
    16921 gcgatatgcg gcacatcggc tttatcggcg aagagtttga cgatttcggc aatgtgttct
    16981 tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg
    17041 atgtcggtat tgtctttgtg tttggacaaa accagtatat tgacggcgat gccggtgccg
    17101 taaaagagat tgggcgcaag ggcgatgacg gtttccacgt agttgccctc caccagatat
    17161 tggcggattt tctgctctgc gccgccgcga tagaaaatgc cggggaatga gacgatggcg
    17221 gcgcggcctc tgccggaaag gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg
    17281 gatttcgggg cgagtacgcc tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg
    17341 ccgctgccta tccagttgat ggaatagggc ggattggaaa cgacggcatc aaagggtttg
    17401 ctgtctttga gtttgggatt ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa
    17461 ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc
    17521 tgcccgaaga agccttcttc gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc
    17581 aggctgcccg agccgcaagc tgggtcgtag attttgttta ctttctcctg cccgtgcacc
    17641 gccagccgcg caatcagctt ggatacgctt tgcggggtga aaaattcgcc gccggatttg
    17701 cctgcgttgg cagcgtagtt ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg
    17761 tggtagtctt caaaactgcc gaaatcgagt tccgccacgc cttttaggac ggcggcaagg
    17821 cgtttgttct tgtcggcaac gatgctgccg agccggctgc tggtggtgtc gaagtcgtca
    17881 aacaggcctt taatgtcttg ttcggacgga tagccggagg cggagctttc aatcgcggta
    17941 aaaatttctt tcagcttggt gttgagctct tcgttttgat gggcttcggc ggcaatattg
    18001 caaaaaagct ggccggggta gatgaaatag cctttgactt tgacggcatc gtctttgatt
    18061 tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc
    18121 atatagtcgg taaagttttc gctgataaag cggtagaaaa gtgtgccgag aacgtattgt
    18181 ttaaagtccc agccgtccac cgcgccgcgt acttcgtcgg caattttcca aatttggcgg
    18241 tgcagttggg cgcgttgttg catttcggtc atcatcgaaa tccatataaa agttaaacaa
    18301 atcgaaatcg cctgatattt tcagacgatt ttttacgggc attcaaatta agccaaattt
    18361 tccagcatcc atttgacgta gcgctcgacg cctgctttga catcgaaaaa ttcttccgta
    18421 taacctgcct cgcgcaattt ggtgatgtcg gcttgggtga agctttggta tttgcctttg
    18481 agcgcgtcgg gaaatgggat gtagcggata aattcttctt ctaccaactc ttttaaactc
    18541 atttcaggtt tgccttcggc ggcgcggcag gcgttgacgg tggcagcggc gagttcgtta
    18601 aactgttggc tgcgacctgt tcctaggttg tagatgccgg aaagttcggg atggtcgaag
    18661 aagtagaggt tgactttggc gacgtcttcg acgctgacga agtcgcgggt ttgttcgccg
    18721 ttgccgtagc catcgttgct gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg
    18781 aaatggtgga aggcgacgga tgccatgcga cctttgtgtt gttcgtgttg tccgtaaaca
    18841 ttgaagtagc ggaagccgac gacttgggcg gtcagccttt ctttcatgcg gcggcgcaat
    18901 acttggtcaa acaggaattt ggaatagccg tacacattaa gcggtttttc cagttcgcgc
    18961 tcttcgcgga agatttcgcc tttgccgtaa accgccgcac tggaggcata aaggaaggga
    19021 atgcgttcgt cctgacacca gtccaacaaa tccagcgtgt actggtagtt gttttccatc
    19081 atatacaaac cgtcgtgatt catggtgtcg gaacacgcgc cttgatggaa aacggcttcg
    19141 atgttttgat aaggtaaaat gtgttccctc acttggcgga tgaattcatg tttgtcgaga
    19201 taatgggcga tttcgcactc ggcaaggttt ttgaattttt cgcctttgct caaattatcg
    19261 acggcaacaa tgtcagtaat accgcgttga ttaagtgctt tgacgatgtt gctgccgata
    19321 aagccggccg cgcctgttac gatgatggtc atattcggtt tcctttgttt tgagtaaaat
    19381 taataaactt agttacaaga aatcatcgat attgtcctga aacacaaatt tgcgatgata
    19441 ttccaaatat ttttctcttc cttcgggata tattctcatc ttattttttg catttggatt
    19501 gatattaagt ttttcccatg tgtaacggct tagtcgagta ccgcaaatca aacgaccatc
    19561 atccaagaaa gtaatataac cttgatcgaa caaccaatca taagtcggcg ttaaagctaa
    19621 cccatttaaa taatctaatg cctctttctc atttttctca gtaatacaaa ccatataagg
    19681 tttaatatga ctagccctta acaaaatctc atctgtaatt aatgtaaatg gacattgcgg
    19741 catatgattt ataacatcct tgcgatattt ctgctgccct tttctacttt tctgtttaat
    19801 gatttcttgc tctacgaatt gattaaattc tgaagccggt atttctaatt tttctcttga
    19861 taagagttgc gggtgcacga tagagcgaaa ttgataatcc aaaaatattc ggaaataaaa
    19921 taatggttct gaatcttcta tatctttgac aggcaatagt ttaagaattg ataaataact
    19981 aattttgggc aatacaatct tacgccaaat tgaccatata tcatcctccg aacggatata
    20041 acccctacgg taacctttag aattttccaa atggtctgat acatcatgga ttgaaaagaa
    20101 aatattttct gtaggcagat tattaatact taaaacgtaa tcatcataaa accttgaaat
    20161 atcatgatga tatttctgtt cttgtttgtg atattcaact tttgcatcaa ccaaataacg
    20221 ctgtaaattt ttcttattaa aaaaacaatt tttctgaatg agaggatatt ctaccctcct
    20281 tccatttttt gtctcagtaa tcccccaatt ttcatactca aaaaatgtac ttagttttcg
    20341 accactatca tcaatataac tacctacata tttgcgtgct tccccattac caccaaaaac
    20401 agataacttg ttattcctgt gaataaaact atcttccgca cgtaaatttt gaatactatc
    20461 gacaataaaa tactctatgt tttcaatagt aattctttga aaatccggca ttttactctc
    20521 cataaagcgt aatatccatt tgttttaata tagcgattaa tacatcaaca acaatactgt
    20581 ttcccgcttg gcgatacatt tgagtatcac taactaaaat tttaaaatca tctctaaaac
    20641 ccattagccg caaacattcc ctaggtgtta atttacgaat acgtccacga ttatgagtaa
    20701 cataattatc aaccccggca cgatgcattt tatgcatagt ttgcaataat gggcgagcta
    20761 caggcaaatc agtttccgta ctggttttaa aattttttgt tcctcctgcc aaaacataat
    20821 ttttgatttt ttcagaaaga taatattttt cctcaacgtt atttacatca aaaataaagt
    20881 catcaaattc agattcgcgg gctgcctgaa aaataaaatc accatgccaa ttaaattgtt
    20941 gatttgcttt ttggcataag gcaatttctc cattaatctg tgtataacgt ttttgtctat
    21001 ttttagaact ggtaacaaat ttcacaccct tttcacgtaa aaaatatttg ctatcagtat
    21061 agtcctccaa aaaatcttgc atagtatgtt ctaattcaat cttttcagga aactgaaaac
    21121 catttatagg aggggtatga aagccaacaa caaaaatacg ctcacgatgt tgaggaatcc
    21181 cataatcctt actattcatt atttggaaat ataagtcata accaagtgaa taaaaaacac
    21241 tttttacaac tttccaagtt tttccattat catgattgag caagcccttt acattttcat
    21301 aaataaatat ctttggttgg acttcatcca caacgcgagc aaattcatag aataatgttc
    21361 ctcgtgtatc ttctaatcct gcacgtttgc caaccatgga aaatgcttgg caaggactgc
    21421 ctccaactaa aatatcaact tgatttctaa actttctcgc atcaaattga gtaatgtcgt
    21481 tatgccaaaa atcttcattt aatttatagt ttccaagata acttttttta acgtatggat
    21541 caatatctcc tgcaaaaaca atggtatggt ttaaatttaa tcggtggaat gcctgttcaa
    21601 ccgcaccaat cccgctgaat acggttgcta atctaatatg tgaatcaggt ttaagaaaag
    21661 ttttagattt ccaaccttgt tgactgggaa agagcaaagt tttttgtaat cgagtatcgt
    21721 gtgtctgtgc cattgtcgaa atagtcatac ttatatcgtt ctgtttatct tatcaatatg
    21781 aaaactacat tgttgattgc cctgacaatg ccttgatcaa ttcggcaaac gagcaaaccg
    21841 ccgtaccgag tttcgccacg acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg
    21901 gcatggtgca gcctgccgcc aaacccaagc ccattccggc aatgacggtg tcgcccgcac
    21961 cggatacgtc gtaaacttct tgggcgcggg tgggctggta aatcggttcg ccttcgctga
    22021 acaaggtcat gccttcttcg cttcgggtca gtaaaacggc ggtcaggtcg aggtggcggc
    22081 gcaggttttg cgctttttcg gtcagctcgc tttcgttttt ccaactgccg accacttctt
    22141 tcaattcggc gcggttaggc gtaatcagag ttgcaccgac atatttttcg taatcgtcgc
    22201 ctttggggtc gattaagacg gttttgccgg cgtgtttcgc ccaatcgatc atatcggaga
    22261 tatgcgacag gccgcctttg ccgtagtctg aaaaaatgat tgcgtcgtat tcgggcaaga
    22321 tttcgcggta tttctgcttg atttgttcca acacttcgca gttgggatgt tcttcaaaat
    22381 caagacggat aagctgctgg ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt
    22441 gtttgtcgcg catcagatag gaggcgacgc cgtcctgcac catcagcgca tcgagcgcgt
    22501 cggcggcttc gtcgttgccg gttacggaca acagccctgc cctgccgccc aacgaagcga
    22561 tgttgcgcgc gacatttgcc gctccgcccg cgcgttggtc gattcgtccg attttcgcca
    22621 ccggcacggg ggcttcgggc gaaatacggg acacatcgcc gaaccaatag cggtcgagca
    22681 tcacgtcgcc gacaaccagg actttggctt gcgcgaaacg ggatttgagg gtttcttgtt
    22741 ggaacttggc ggacatattg cctccgttgt attttttcag acggcctcag cctgtaaggc
    22801 cgtctgaaaa tcagtttgct tcaaggtttg ccacgcggtt cacttcgcgc aatacggcta
    22861 ccggatcggc agcttgggtt acaggacgac ccattaccaa ataagtcgaa gcggcagcca
    22921 aggcttcggc cggtgtcatg atgcggcgct ggtcatcatt attgccggca acgtccaagc
    22981 ggatgccggg cgtgaccaag acaaaatctt gtcccaactc gcggcgcagc ggtgcggctt
    23041 cttgggcgga acagaccacg ccgtccaagc ccgaactttg cgccagtttt gccaagcgga
    23101 tgacttgttc ttcaggggcg gtgttcaaac cgatttccgc caaatcgctt tgttccatgc
    23161 tggtcaacac ggttacgccg attaagagcg gctttgtacc gtatccggca acggcttctg
    23221 ctgcggcttc catcatacgg cggccgcccg atgcgtgcat atcgaccatc caaacgccca
    23281 tatcggcagc gactttgcag gcttgcgcga cggtgtgggg aatatcgtgg tatttcaaat
    23341 cgaggaaaag tttgaaacct tgattgatta agctttctgc caaattgcgt cccgtcgcgg
    23401 taaacagctc tttgccgatt ttgatttgac acaatgtcgg gtcaaggttg cggacgaatc
    23461 cgagcgtgtc tttttcgttg gaaaaatcaa gggcgacgat gacgggggtg cgttgttgcg
    23521 gagtttggaa gtcggagatt aaaggattca tggtgttttc gtttcgggtt ttcagacgac
    23581 ctttatcgtc tttttcgcag tatagtggat taactttaaa ccggtacggc gttgtctcgc
    23641 cttagttcaa agagaacgct tctctaaggt gctgaagcac aaagtgaatc ggttccgtac
    23701 tatctgtact gcctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataacaa
    23761 cgtaaattca aaccgtaggt cgggcattga tgtccgacct acgcggactc aagcctccga
    23821 caaactgttt cggataaatt tgaaccggta catcagccgt cattcccgcg caggcgggaa
    23881 tccattgata aaactcagtt catcagattt aaacagcgat tgcccaaatt cagaaatgga
    23941 ttcccgcctg cgcgggaatg acgccaaatg attatttttc ggttgcaaca cgctgtttat
    24001 caaaaacagg tcgtctgaaa acctgatttt ctgttttcag agtgcgaagc ctgcccgtgg
    24061 gtacgacctt ttcgatttat ttgcccaatg cgcccaatac ttcggctttc acggcttcta
    24121 ccggctgagt gccgtcaact ttgatgtatt taggcgcgtg ttcgccttcc agtttgctgt
    24181 aaaaatcgac caaaacttcg gtttgctcgt ggtaaacggc aaggcgtttt ttcacggttt
    24241 cttctttgtc gtcgtcgcgc tgaatcaaat cttcgccggt tacgtcgtct ttgccttcaa
    24301 ctttgggcgg gttgtaggta acgtggtaag tacggccgga agccaaatgc acgcggcggc
    24361 cgctcatacg gtcgacaatc acgctgtcag gcacgtcgat ttcaacgact gcatccaaat
    24421 ccacgcctgc ttcaaccatc gcttcggctt gtgccaatgt gcgcgggaaa ccgtcgaaca
    24481 ggaaaccgtt tttgcagtcg tcttgcgcga tgcgttcttt gaccatgccg ataatgatgt
    24541 cgtcgcgcac caagccgcct tcgtcaatga tttttttcgc ttccaaaccc aagggcgtgc
    24601 cagccttaat cgcggcgcgg agcatgtcgc cggtagagat ttgcggaatg ccgaacgcgg
    24661 cggtgatgaa ttgcgcctga gtgcctttgc ccgcgcccgg cgcgcctaaa agtaatgctt
    24721 tcatgaagtg atcctttgaa atgtgtttat tggaatcaaa cagatggttt acgattctac
    24781 acggcttggc acaatagttc aaaaaccaac tgcacaaact gccatttgca agcaaatgtt
    24841 ttgcatacct ttgccgagtc tgatattcac aagccggcaa ctttttggga caatccccaa
    24901 aatttcaaaa catcacaccc taccaaaaag gaacatccgt gaaacgaatt tttctgtttt
    24961 tggctaccaa tatcgctgtt ttggtcgtaa tcaacattgt tttggcggtt ttgggcatca
    25021 acagccaggg cggcacgggc agcctgttgg cgtattccgc cgtcgtcggc ttcactggtt
    25081 cgattatttc gctgctgatg tccaaattca tcgccaaaca atcggtcggc gcggaagtta
    25141 tcgacacgcc gcgcaccgaa gaagaagcct ggcttttgaa cactgtcgaa gcccaagcgc
    25201 ggcaatggaa cctgaaaacg cccgaagtcg ccatctacca ctcccccgaa cccaatgcct
    25261 ttgccacggg cgcatcgaga aacagctccc tgatcgccgt cagcaccggt ttgctcgacc
    25321 atatgacgcg tgacgaagtg gaagccgtat tggcgcacga aatggcacac gtcggcaacg
    25381 gcgatatggt tacgctgacg ctgattcaag gcgtggtcaa tacctttgtc gtgttcctgt
    25441 cgcgcattat tgccaacctg attgcccgaa acaacgacgg cagccagtcc cagggaactt
    25501 atttcctggt cagcatggta ttccaaatcc tgttcggctt ccttgccagc ttaattgtca
    25561 tgtggttcag ccgacaacgc gaataccgcg ccgacgcggg cgcggcaaaa ctggtcggcg
    25621 cgccgaaaat gatttccgcc ctgcaaaggc ttaaaggcaa cccggtcgat ttgcccgaag
    25681 aaatgaacgc aatgggcatc gccggagata cgcgcgactc cctgctcagc acccaccctt
    25741 cgctggacaa ccgaatcgcc cgcctcaaat cgctttaaac cgatttgaaa cggcaaaaac
    25801 cgtacgcgca agcagtacgg ttttttgtat atgctgtctg aaaacatcag gtttccatac
    25861 cgacaccaac cgtatccatt tccgcaccgg atacgccgca ttgatatacc ctgataaacc
    25921 gtatccggac agaaaatata gtggattaac aaaaaccagt acggcgttgc ctcgccttag
    25981 ctcaaagaaa acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt
    26041 atactgtctg cggcttcgtc gccttgtcct gatttttgtt aatcccctat atcagataaa
    26101 acagcggtaa aattgccgtc tgaaaccaac tctgcgccat acgggacacg ctctgcacgt
    26161 ctatttgcct ccgatatatc ctccctactg taggaacgtc tgccgatttg cacgaaatcg
    26221 gcacctctag cacccttcag gatggcggac gttatccgtt cttgggattc tgcaagacgg
    26281 tcgacggcat tatacagacg ctcggtctga tacccttccg aagcaacctg cttcaggtat
    26341 tccaaataac cgtaacccaa cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt
    26401 gccgaacgga ttttaggggg catatcttga agcatatctc taccgaaccg aaccaaagtc
    26461 tctttaaagt cggactctac acgcgtagaa ccctcggaaa tcttgaagtt caactcatac
    26521 tgcttccgtt tgtccgctgt caaccgcttg aggttgtcat ccccgtattg gacggttgac
    26581 acaaccctgt acagactgtc ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata
    26641 actggattcc caacccttgc cgccgattct gatactcaaa acggtatcca ggcgttccag
    26701 cagggcttcg ggagacagca gtgcgtattc ttcaatattt tccataggtt gattatagca
    26761 gaaccttata cctgttacag acaaggttgt cgggatttga acgtccgtcg agcttggttc
    26821 gtaggattta gggtgttgtg aaccttacct atcgtaccca agcactttgt acctgtttga
    26881 aataaggttg tcgggcgacg ttcgaccctt gagcctgtga ttgatttgtt ggaaatgtac
    26941 acctaaagca gcacctgcgg cagtaacgct tgggaacgtg agtgtttcgt tggtcgttgt
    27001 gtcgaggatg aggaggggct tccctacatt tggaggtgtt tgggtttttg acatcttagc
    27061 acactcagga cacccgtact ttgactttat aagggcatta aatgtcgaat aacgaacgtt
    27121 cccgtgcata ggacaattaa cggttactgg gtaattcaca ccgtcaaagt cggttagaga
    27181 gatattaggg aatatatcag taagttcaga agctgcttgg ttgaaagatt tggccatttg
    27241 agactccaaa attgattgtc aaatggattt aatagcaaat ttataaaaat gtcaaatttt
    27301 tcgagaagca cctgatagtg gcgaggggta tacactgtta aaaagtgtat acgtcattta
    27361 gtgcagatgt gaaccttata cctccctgca agtaaagtct ctgtgtgaac ccgacctttt
    27421 atacggtcag tgatagctgc tgggtttata cccaatgacc ttgcagcaga tgctcttgag
    27481 gggaatgtaa gtgtttcgcc agttacagta tcctccaata ctgtattttt agataattta
    27541 tctattgtat cttctggaat tttgtgggaa ttgacaccat aagatgcaca ttttggacac
    27601 ccacttttag agtccaacat acttttgaat gtagagcaag taaccctccc gtgtatagga
    27661 cagacgatta ccgtcgggta acgaacgcca ttgaactcca caagattcaa atgtggaaat
    27721 ttagaggtta gcttagaaac tgcttgagta aatgtaagtg ccatagtggt tgtgaactta
    27781 ctttaccaac ctataacgat acctaatcaa tcggtcaaac ggagtgcgtc cgcttaaacg
    27841 tacagctaaa gtgctgttgg agcaaccgat aaatttactg gcagccagtg tacttgcaaa
    27901 ctcgtagatt tcccctgtct ctgtgtcctc caacctaatg gcaacggcgt ttggagaggg
    27961 cttttcggga tttcgagtac attcaggaca tcctgttgag gatttcatgg cagcttgtag
    28021 attcccgtaa acaaccgacc catgcaaagg gcattggatt gttaccggtt cgtagttttt
    28081 agtgaacttc aaaaccgtct tatcaggaaa tttactggaa aaccttaact gaacatcttg
    28141 tagcgagagt gattttggca ttatagacct ttctaaaatg aaagcattat acaaccaaaa
    28201 tatagaaatt ttcaaatttt ctctgaacgc actggtatcc gatggagcta aatgtaaaga
    28261 aaaatggtat acgtctttac taaatcttta cacattttaa ctgtatagtg tttgacaaac
    28321 aacaggcata ggcgcataat gcacccgtag ttagatagat agtaaggaga taaccaatga
    28381 cttagcacct gcccccctct ctctcgcgca ccccgcccct cgcttgcggc taatgggctt
    28441 tgcgcgacat atacactttc atctcctgca tggcttaact gcctatgcaa tagtacacac
    28501 atcaacaaat caggctggca cgtcttggca ttggctgacg taccattgta gcccgaaatg
    28561 ttgataccct actatttatg accaacggcg gattagggct tcagacggca attttactgc
    28621 aaaaccttat gcgaacggat gatgcagcac aatcgtttct tcgcggtcgg ggccggtgga
    28681 gacgatggcg accggcgcac cgcagacttc ttcaatccgt ttcaaatatg ctttggcatt
    28741 ggcaggtaat gcgtcgtagc ttttcacgcc gaaagtggat tcgctccaac cgggcatggt
    28801 ttcgtaaatc ggcttgcagg cttctaccgc atcagagccg caaggcagga tgtcggtttt
    28861 gccgccgtcg ggcaattcgt agccgacgca gatattgatg gtttcaacgc cgtccattac
    28921 atcgagtttg gtaatacaca taccggaaat gccgttgatt tggatggagc gtttcagggc
    28981 ggcggcatca aaccagccgc agcggcgtgc gcgtccggtt accgaaccga attcgtgtcc
    29041 gcgttctgcc aaacctgcgc cgacttcgtc gaacaattcg gtcgggaacg ggcccgaacc
    29101 gacgcgcgtg gtataggctt tgacgatgcc caaaacataa tccagcattt gagggcctac
    29161 gcccgcgcct gccgaagccg cgccggcgag gcagttggac gaagtaacga aggggtaagt
    29221 gccgtagtcg atgtccaaca acgcaccttg cgcgccttca aacagcagtt tttcgccgtt
    29281 tttgtttttt tcgttcaaca cgcgggacac gtcggtaatc atcggcgtaa tgcgcggcgc
    29341 gactttttcg ataaccgcca tcacgtcttc cgctttcacc gactcggcat tgtgcagatg
    29401 ttgcagttgg acgttgtaat aagcaaggac ggcatccagt ttttcacgca gtttttcagg
    29461 atgcagcaaa tcggcggcgc gaatggcgcg gcgtgccact ttgtcttcgt aggcagggcc
    29521 gatgccgcgg ccggtcgtgc cgattttgcc tttgccgcgc gatgcttctc gggcttggtc
    29581 gagcgcgatg tgataaggca ggatcagcgg gcaggtcggc gcgattttca gacggccttc
    29641 gacgtttttc acgcctgccg cgttcaactc gtcgatttcg cccaacaggg cttcggggga
    29701 gacgacaacg cccgaaccga tgaagcagtc cagactttca tgcaggatgc cgctcggaat
    29761 caggcgcaaa atggtttttt tgccgccgac aaccaaggta tgacccgcat tgtggccgcc
    29821 ttggaagcgc accacgcctc cggcttcttc cgccagccag tcaacgattt tacctttacc
    29881 ctcgtcgccc cactgtgcgc cgattactac aacattttta gccatagcca tataaccttc
    29941 gatattaaaa attattgcgg aaatccccaa accggtgcgg ctttgcgccc cgccacgatt
    30001 tgcctttcat ccgccatgaa cgggtattta gcgtttcacg acctgccaaa cgccgtccgt
    30061 ctttttcaga cggcctgcaa gctcttccga aacattgtgt ccgataccgt aatcgattac
    30121 gacacactgc ccttgttcac gcaaggcttc gaccgcttcg tgcgccgctt cggcatcttc
    30181 cgcatcgacc aacacggcgg gctgccgttc gatggcgggc aaacgcccga taaagctgcg
    30241 caagtcgaaa ctgaatcccg ttgccgggcg cgccctgccg aaatatccgc ccaatccgtc
    30301 ataacgcccg ccgcgcgcga ccgcgtcgtg gaaatcggcg gcataggcgg catacagcaa
    30361 gcccgtgtgg taattgtcga cgcgcagctc ggacaagtcg atatggattt cacaatcggg
    30421 gaatgcgtcg cacaccgcct gcaattcgcc caacgcgccg ccgaccgccg acaaatccgg
    30481 caaccgtccg cgcgcgtcgg acaacacttc acgcccgccg tacaggcgcg gcagcagcga
    30541 gaatgctttt gcccacatac cgtccagctt ccaagccttg acctgcgctt cgaccgtctc
    30601 ggtatcttta tcctgcatca aggcaagcag cgttgcagac tgccccgcat ccaaatgcgc
    30661 cgcatcggac aaggcgcgaa atatgccgat atgccccagc gaaagcagca ctttgcccat
    30721 atcggcaatt ttcatgcttt tcagcatcag gtctatcagc tcgatgtcgc cacggatgtc
    30781 ggcaaaaccg tacatttccg cccctgcctg caagggttcg cgcatattca gcagaccgtc
    30841 ggactgcgcg tgcaacaccg gaccggcata acacaaccgg ttaatccctt ggttggcgga
    30901 taaaagatgg gcatcgatac gcgccacctg cggcgtgatg tcggcgcgta tgcccaactg
    30961 cctgccgctg agcctgtccg ttaccaaaat ggttttcagg gaaagccccg catcgatatg
    31021 cgtcagcagg gaatgtgcgt actccatcag cggaggctgt accagttcat aaccgtgtac
    31081 gcggaacagt gccaacaact gctccctcgc gctttcaagc tgccgcgcgt tcgtgggcag
    31141 tacgtcggcg atatgttccg gaagctgcca tgtctgcatg agaatctgcc ttctatttta
    31201 tcatgtcaat acaaaaggct gcccgatccg caatcagacg gcatctttgc cgcaacgcat
    31261 cacgccgctg tcaaaacggc gatgcttcct tccgattcag aatccgaaca gcccctgtcc
    31321 gacggctcaa tgccgtcaag tcgttaaaac caaactttac cataaaatac acacaatctg
    31381 aatatacttt tataccctgt atccgccgtc tgacggcacg gcattttttt actatataat
    31441 gtgcaccatt atttctcatc acttcctgct gccgttttct gcttcagacg gcatttttgt
    31501 tgattgaaca cttatgctgc tcgacctcaa ccgcttttcc ttttccgtct tcctgaaaga
    31561 agtccgcctg ctgaccgccc ttgccctgcc catgctgttg gcgcaggtcg cgcaggtggg
    31621 catcggtttt atcgatactg tgatggcggg tggtgcaggc aaggaagact tggcggcggt
    31681 ggctttgggc agcagcgcgt ttgccacggt ttatattacc tttatgggca ttatggcggc
    31741 gctgaacccg atgattgccc agctttacgg cgcgggtaaa accgacgaag tgggcgaaac
    31801 ggggcggcag gggatttggt tcgggctgtt tttgggcgtg ttcggcacgg tattgatgtg
    31861 ggcggcgatt acgccgttcc gcaactggct gaccttgagc gattatgtgg aaggcacaat
    31921 ggcgcagtat atgctgttca ccagcttggc gatgccggcg gcaatggtac accgcgcact
    31981 gcacgcctac gcctccagcc tgaaccgccc gcgcctgatt atgttggtca gctttgcggc
    32041 gtttgtgttg aacgtgccgc tgaactatat tttcgtttac ggcaaattcg gtatgcccgc
    32101 tttgggcggc gcaggctgcg gactggcgac gatggcagtg ttgtggttca gcgcgctggc
    32161 attgtggatt tatatcgcca aggaaaattt cttccgccca ttcggactga cggcgaaatt
    32221 cggcaaaccg gattgggcgg tgttcaaaca gatttggaaa atcggcgcac ccatcgggct
    32281 gtcttatttt ttggaagcca gcgcgttttc gtttatcgtg tttttgattg cgcctttcgg
    32341 cgaggattat gtggcggcgc agcaggtcgg catcagtttg tcggggattc tctatatgat
    32401 tccgcaaagc gtcggctcgg cggggacggt gcgcatcggc ttttcgcttg ggcggcgcga
    32461 attttcgcgg gcgcgttata tttcgggcgt gtcactggtg ttaggatgga tgctcgccgt
    32521 gattaccgtg ctttccttgg tattattccg ttcgccgctg gtaagtatgt acaacaatga
    32581 tccggcggtt ttaagcatcg ccgccaccgt cttactgttc gccggcttgt tccaaccggc
    32641 agacttcacc caatgtatcg cctcctacgc cttgcgcggc tacaaagtta caaaggtgcc
    32701 gatgttcatc cacgccgccg ccttttgggg ctgcggtctg ctgccgggct acctgctcgc
    32761 ctaccgtttc gatatgggca tttacggctt ctggacggca ttgattgcct cgctcaccat
    32821 cgccgccatc gccttggtgt ggtgcttgga attgtgcagt agggagatgg tcagatcgca
    32881 taaggctgtc tgaaaactcc gccgattaaa aatatagtgg attaacaaaa accagtacgg
    32941 cgttacctcg ccttgccgta ctatttgtac tgtctgcggc ttcgttgcct tgtcctgatt
    33001 tttgttaatc cactatagaa acgatgggct gaagcccgcc actggcaata ctcattttaa
    33061 agttcaaacc catgcaaccc atccgatacc gaaccgacct taccccctac aacaccttcg
    33121 gtcttcgcgc ccaagcccga gcctttatcg cgctcgaaca tgccgacgag ttgtgcgaca
    33181 tcgtccgact gcccgagttc gaccgcgata ctgttttatg gctgggcggc ggcagcaata
    33241 tccttttgat ggaggactac gccggtctgg tcgtacatat ggaaaacaaa ggcatacgcg
    33301 aaattgctcg ttcagacggc atggttctga ttgaagcgca ggcgggcgaa atctggcacg
    33361 attttgtcct gcacaccgtc gcgctgggtt tgaacggttt ggaaaacctg agcctgattc
    33421 cgggtacggt cggcgcgtcg cccgtgcaga acatcggcgc atacggcgta gaggcgaaag
    33481 atgtgattca cagcgtgcgc tgcttcgatt tggatacgga aacctttgta accctttcca
    33541 atgccgactg ccgcttcgcc taccgcgaaa gcctgttcaa gcaggagggt aaagggcgtt
    33601 atgtgattgt ttcggtcgta tttgcattaa aaacgcattt tgtgccgact ttgggttacg
    33661 gcgatttggc ggccgccgtt gccgaactga gcgcgggcag ggtcccgacg gcgaaagatg
    33721 tttccgatgc agtgtgtgca atccgcaaca gtaaacttcc taatcctaac gtgcttggca
    33781 atgtcggcag tttctttaaa aaccccgtcg tcagcgcaga aaaagccgcc accttgttgc
    33841 agcggcatcc tgatatgccg cattatccgc agccggacgg ttcggtcaaa ctcgccgccg
    33901 gctggctgat cgaccaatgc cgtctgaaag gcttccaaat cggcggcgcg gcggtacacg
    33961 acaaacaggc attggttttg gtaaacaaaa acaacgcctc ggcaaacgat gtccggcagt
    34021 tggcgcaaca catcaaattt acagtatttg ctcggtttca ggtagaattg cacgccgaac
    34081 ccaattggct gcctgcttcg ttcagcctgt aaatccaagg agtatgcccg tgacccgccc
    34141 cgccaaaatc aatacttaca cacgcatcat cgacgccagc cttgcgcttt tcaacgagga
    34201 aggcgagcgc aacatcagca ccaaccatat tgccgcccac ttgggcatca gtccgggcaa
    34261 tctctattac cacttccgca acaaagacga aatcatcgtc caactgttca aacgttacag
    34321 cgaagccctg ctggaatacc tcaacgaagc cgtgttgccg tctgatgtgg aagactccat
    34381 caactatatg gcgggcattt acgacgtgat gtgggaatac cgcttcctat tcagcgacgt
    34441 gaacaccctg cttgcgcgca gtgctgaatt attgggcgaa cacaatacct tcacccaagc
    34501 caaagtctcc ccgctcttgg tcaacctgct tacccagctc aacggcttga acatcatcca
    34561 agccgaccaa accgctatga acgacctcgc cgtcaatatg tggatggtca cgaaatactg
    34621 gttcgacttc gacagctccc tgcgcggccg caccaagctg accgaagact ccaaagcacg
    34681 cggcgtccgt cgtaccttaa gcctccttcg tccttatctt ttgcccgaac accgcgagaa
    34741 atacgaccgg aaaatcggca acggcaaccc gtaaacccac agcgtccgaa tcagccttca
    34801 gacggcactt acccgacggt atcagcgaat gcgcgcagtc cccccgcccc atatctccat
    34861 cacaggccta ggttacctcg gcctgccgct ggcacaaaag ttttaccaac acggcagccg
    34921 cgtcgccgcc ataaaacgca gcctgacttc ggacgacatc aacctgccca tacatctcga
    34981 caccttcgac ctcggcagca ccgatgcctt tcagacggca ttgtggcggc accacgccga
    35041 caaacccgtc tggttcttcc tgctaccgcc ctcctcgctg acgcattacg ccgacaccgt
    35101 caaacaatgg gcagaacttg cccgggcgtg caacgtgcaa cacctgattt tcacaagcag
    35161 caccagcgtt tacggcgata cagcgcgcga atgcgacgaa accgccacac ccgatccgca
    35221 aaccgagtcc gcccgccaaa tcctcgccgc cgaacaacac ctgctcgaca gcggcatctc
    35281 caacatcgac atcctgcgac ttggtgggct ttattgcgcc gaacgccatc ccgtcagccg
    35341 tctcgttcaa aagcaaaaca tcccaggcgg caaccgcccc atcaacatcg tccaccgtaa
    35401 tatcgccgtc gaaagcctgt ttcagacggc atttaacccc ggcggcaggc ggctgaaaaa
    35461 cattatcgaa ccgcgccacc cgacacgacg cgaattctat acggaagaag ccgccaaact
    35521 cggcttgccg gctcctgatt tcgcacccga cgacagcgtg ggcaaaatca tccgtaccgt
    35581 ttgcgataac ggcttaagcc tgtaaaataa gcgtcaacag caacaaacag gcattctccg
    35641 ccccgatcag aaaatatgtc cgccctcctc cccatcatca accgcctgat tctgcaaagc
    35701 ccggacagcc gctcggaact tgccgccttc gcaggcaaaa cactgaccct gaacattgcc
    35761 gggttgaaac tggcgggacg catcacggaa gacggtttgc tctcggcggg aaacggcttt
    35821 gcagacaccg aaatcacctt ccgcaacagc gcggtacaga aaatcctcca aggcggcgaa
    35881 cccggggcgg gcgacatcgg gctcgaaggc gacctcatcc tcggcatcgc ggtactgtcc
    35941 ctgctcggca gcctgcgttc ccgcgcatcg gacgaattgg cacggatttt cggcacgcag
    36001 gcagacatcg gcagccgtgc cgccgacatc ggacacggca tcaaacaaat cggcaggaac
    36061 atcgccgaac aaatcggcgg attttcccgc gaatccgagt ccgcaaacat cggcaacgaa
    36121 gcccttgccg actgcctcga cgaaataagc agactgcgcg acggcgtggt acgcctcaac
    36181 gaacgcctcg accggctcga acgcgatatt tggatagact aaccttcaga cggcatccga
    36241 catgaacagc ccttttcaca acatcggcat cgtaacccgc cccaacacgc ccgacatcca
    36301 agacaccgcg cacacgctga ttaccttttt gaagcagcac ggctttaccg tctatctcga
    36361 cgaagtcggc ataaaggaag gctgcatcta tacccaagac accgtcggct gccatatcgt
    36421 caacaagacc gaactggggc aatactgcga cctggtcgcc gttttaggcg gagacggcac
    36481 ctttctctcc gtcgcccgcg aaatcgccct gcgcgccgtt ccgattatcg gcatcaacca
    36541 agggcatttg ggcttcctga cccaaattcc ccgcgaatat atgacggaca agctattgcc
    36601 cgttttagaa gggaaatacc ttgccgaaga gcgcatcctg attgaggccg cactcatccg
    36661 cgaaggcaaa accgccgaac gcgccatcgc cctcaacgat gccgtcctct cccgtggcgg
    36721 cgctggacag atgattgagt ttgaagtctt cgtcaatcgg gaattcgtct atacccagcg
    36781 ttcggacggg ctgattgtct ccacccccac cggatcgacc gcctattcgc ttgccgccgg
    36841 cggccccatc atgcaggcag gattacacgc cttcacactc gtacccatct gcccgcaatc
    36901 catgaccaac cgccccatcg ccattcccga cacgtctgaa atcgaaatcc tcgttaccca
    36961 aggcggcgac gcgcgcgtcc atttcgacgg tcaaacccat atcgacgtgc aaaacctcga
    37021 ccgcatcacc atccgccgct accgaaatcc cttacgcatt ttgcacccga ccgactacca
    37081 atatttcaaa accctgcgcc aaaaactgca ctggggcgaa caattggtct gaaccgaccc
    37141 ttaccggaaa acacctatga atccaaaaac tccccccgac catacagaag atgccgtgcg
    37201 cctctccaaa cgcatggcgc aactggggct ttgttcgcgc cgcgaagccg acggctacat
    37261 cgaacagggc tgggtaacgg tcaacggcaa aaccgccgta ctcggtcaga aagtttcacc
    37321 ggcagaccgt atcgaactga acaagaaagc ccacgaacag caggcggcac gcattaccat
    37381 cctgttgaac aaacccgtcg gctatgtcag cgcacaagcg gaaaaaggct ataaatccgc
    37441 cgccgaactg attacccctg aaaatcactg ggaaggcgat accggccgca tccgtttcga
    37501 tccgaaacac aaaatcggcc tcgcccccgc cggcaggctg gacatcgact cggtcggatt
    37561 gctggtattg actcaggacg gccgtatcgc caagcagctt atcggcgaaa acagcggcag
    37621 tgaaaaagaa tatttggtgc gcgtgcgcgg caaattggac gaaaaaggac ttgccttact
    37681 gaatcacgga ttgagtttgg acggcgagaa actgcgtccc gcccaagtag aatggcaaaa
    37741 cgaagaccaa ctgcgtttcg tattgaaaca aggtaaaaag cggcaaatcc gccgtatgtg
    37801 cgaactggtc ggactgcgcg tcgtcgggct gaaacgcatc cgcatgggta aggtcaagct
    37861 cggcaggctg ccgcccggca aatggcgtta tcttgctccc ggcgaatcgt tttaaataag
    37921 cattaaaaaa tgccgtctga acgtccttac gttttcagac ggcattgcct tatgcccaaa
    37981 taacggtttc ttccaagggc ctgcgcgctt tcgtggcgat ttcctgaacg cggtagccga
    38041 atgtcgcggc aacggacacg ccccattctg ccgcatcgaa caaaccgaac tgcccggaca
    38101 atacgcgttc catatcggca tagttgaaac cttccaccgg gcaggaatcg atacctgcca
    38161 tcgccgcacc cgtcatcatg ttgcccaacg cgatataggt ctggcggcaa caccagtcaa
    38221 acaaggcgcg cgaatcgtcc aaaatcttga tgtcgtcggc ttgaaacgcc tgatacctcg
    38281 ccaaagattt tgccatggca tccggttcgg taacgccgcg ccgtttgagg ctttccaaca
    38341 taaacgggct gtcgaagcgg gcatttttct ttgccaaaaa caccaccaaa tgactggcgg
    38401 tatccaaagc atccgccata ccccaagaaa acggcttgat tgcctgtcgg atttcggggt
    38461 tttgaaccac aacaaactgc caaggctccg aaccgaccga actgggcgac aaacgcccga
    38521 gttctaaaat aaactgaaaa tcctcggcac tgattttacg tgccgcatcg taatgccggc
    38581 atgatttgcg gtttttaaat gcggatagaa cctgctcttt gcttaatact gccatcgcac
    38641 atcctttcat tacttgtatt gcaaattgat ttcaaaccga tacggcactg ccgcaccata
    38701 caatccgtcc tcgccatcaa gcttcatccg gactttaccc atctctctac ggctgggaaa
    38761 acaaagcggc ggcagactcg ggaataacgc cccaatgcct ttcagacggc atttgggcat
    38821 cattgcaaac gcgcctacgg gtaggcgata taggcataag ccgaatggtt gtggatagac
    38881 tcgaaattct cgctctcgac aacgaaactc ttgatgcgtt tgtcggcgat cagcgaagta
    38941 gcgacatcgc gcaccatatc ttccacgaat ttcgggtttt cgtaggcctt ttcggtaacg
    39001 tatttttcat cggggcgttt gagcaggccg tagagttggc agctcgcctg cgcctccaca
    39061 taatcgatga cttcctcgat accgacttcg gcatcggcag tcaggctgac ggtaacgtgc
    39121 gaacgctgat tatgcgcgcc gtattgggaa atttctttgg aacacgggca aagcgaggtt
    39181 acgggaatca tgaccttcat actgtggccg tatgccccgt ctttgatttc gcccgtgaga
    39241 cagacatcat aatccagtaa ggaccgaata ccggaaaccg gcgcggtttt cttgcggaaa
    39301 aacggaaaag aaacgctgat tttgccggcg cgggaatcta aaagcgcgac catttcggta
    39361 gtcagcttgc gcaattgtgc aaaatccaag gcttcggcat gttgctccat caatgcgaca
    39421 aaacgcgaca tatgcgtccc cttctgctca gcaggcaggt aaaccgtcat ggtcagacgg
    39481 gcaatggtgg actggatgcc ttctgcagtt tgcagggtaa tcggaaagcg caggtctttg
    39541 ataccgacct gattgatcgg cagattgcgc aaatctcggc tggattgcac gtctgcaata
    39601 gtgttcatga gttgtttttc cttaatattc atgtaattgt tgagatgtgt gcatggtatg
    39661 gcgtttccgc cggggaatat accgtcaatc tgcaaatgcg agattatatc actcgctttt
    39721 agcagctgca ttgattgttt ctatttttca gatagtgata aaatcttcga ttattcacat
    39781 ctacataggc agagaccaaa tgaaattcgc cacgaaagcc attcattcca gctacgattg
    39841 cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa aacagtatgt ttgcgttgca
    39901 cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg agcaacccga cccgtcagat
    39961 tttagaagac accgttgccg atttggaaca cggtgcggca ggttttgcgt tttccagcgg
    40021 tatggcggga attgatgccg tatggcgcac tttcctgcgc ccgggcgata ccattgtcgc
    40081 cgtcgccgat atttacggcg gcgcttatga tttattggtc gatgtttatc aaaaatgggg
    40141 ggtgaacgtt gtttttgccg atttaggcaa tccggataat ttggacgaac tgcttaaagc
    40201 gcacaaggtc aaactggttt ggctggaaac gccgtccaat ccacttttac gcttggtaga
    40261 catcaaagtc cttgccgcaa aagccaaagc agccggtgcg ctggtcggta tcgacaacac
    40321 ttttgccacg ccgtatctgc aacagccgtt ggatatgggt tgcgattttg tattccattc
    40381 cgctaccaaa tatttgtgcg gccattccga cgtgttgatg ggcatcgtcg ttgccaaaac
    40441 caaagaactg gcgcagcctt tgcacgatat gatggtgcat accggcgcgg ttgccggccc
    40501 gctggactgc tggctggtgt tgcgcggcat caaaacactg gctctgcgca tgaacgccca
    40561 ttgccaaaac gcactcgaaa tcgcgcgccg tttggaagcc catcctgcca ttgaaaaagt
    40621 gttccatccc ggcctgccgt ctcacgaaca ttacgaactg gcgaaaacac aaatgcccaa
    40681 aggcatcggc ggcgtggtta cggtttatct caaaaacgac acgcgtgaag cggcaaacag
    40741 cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc ctcggcggtg tggaaagttt
    40801 ggtcaaccat tgctattccc agtcccacag cggcgtaccg catgatgtga aaatggaaat
    40861 gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc gaagacgcgg acgatatttg
    40921 gaacgatatt tccgccgcac tcgatacgac tttgtaaact gtaaaaatgc cgtctgaaac
    40981 catggtttca gacggcattt caattaaccc ggccgaaaat caacgcttca acatctttgc
    41041 cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc gcacgtttga atgccagcag
    41101 gctttccaaa accactttgc cgccgtccag ccagccgttg gcaatcgctg cctgcaacat
    41161 cgcgtattct cccgaaacct gataggcata agtcggtacg ccgaactcgt ccttcacgcg
    41221 gcggacaacg tccaaatacg gtaaaccggg cttcaccatc accatatccg caccttcccg
    41281 aatatcgagc gccacttcat tcagcgcctc atcggtattt gccggatcca tctggtaggt
    41341 tttcttgtcc gccttgccca aattgcccga actgcccacc gcatcgcgga acggcccgta
    41401 aaacgccgaa gcatatttgg cagaatacgc cataatccgc gtatggatat gcccggcatc
    41461 ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc atatcggaag gggcaaccac
    41521 ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc aaaacctcta cggtttcatc
    41581 gttcatcaca taaccgtttt cgtccgtcag cccgtcctga ccgtgaaccg tataaggatc
    41641 gagcgcgaca tccgtcataa tgcccagttc gggaaacctc tcgcgcaagg cgcggacagt
    41701 tgacggcacg agtccttcgg gattgtacgc ctcctgcgca cgctcggttt tgtttgccgt
    41761 aaccacgggg aacagtgcca acatcggaat accgagcttt accgcctctt ccgccgtaaa
    41821 cagcagcctg tccaaacttt gacgcttcac acccggcata gaaggcacat cctcctcgcg
    41881 cgccgacccc tccaatacga acaccggata aatcaaatca tcggcggtca gcgtgtgttc
    41941 gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg cgcatacgcg aagccggaac
    42001 attgcggtaa ggaaactgca taagccctcc aatcatcctg tcaacaattc aaaccatacg
    42061 gaagccgccg caacttgcag acagcatcgg gcggcaatca aaatccgttt tcaccggaaa
    42121 gggtttcggc ccgccgaaat gccgcgccga aggacggcgt cccgcccatt aaagaacaat
    42181 agcaaaacaa cagcttcagg ctgtcagcct tcgatattct tacgccacaa taccaaaagt
    42241 ttcccgatat gctgaacagg ttgcgcgtca accgcctcac ataaggcagt gcagatttcg
    42301 atacgttcgg cacggtcgtc gccgaatacg cgcactttaa tcagctcatg cgccgtcaat
    42361 gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct gctgaccgac catcacaaca
    42421 ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt ctttggtgtt caatttggta
    42481 tccgtcattt tcctactgct tgaataaaaa gaataatgcg gcattgtacg cgatttggca
    42541 cgacatcggc aaaattaacg tacaatacgc ggtttccatt ccgacccgcg aaccactatg
    42601 gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac acgtcaacga ccactacgtc
    42661 catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat acaaactttt ggaaatcaac
    42721 gaaaaagaca aattaatcaa acccggcacg gtacttgccg acttgggcag cgcaccggga
    42781 ggctggtcgc aggttgccgc caagctgacg ggtacttccg gagcagtttt cgccttggac
    42841 atcctgccta tggaagccat agggggcgtc tccttcattc agggcgactt ccgcgagaac
    42901 gacgtactgg cacaattcga aaccttgttg gacaaccgcc cgctcgacct tgtaatttgc
    42961 gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc aggcacgcag cttttatctg
    43021 tgcgaactgg ctttagactt cgcctcgcaa cacctgaaaa ccggcggcag ctttttggtc
    43081 aaagtctttc agggtgcagg ctatcaggaa tacatggcag ccatgcgcga aattttcggc
    43141 acggtgcaga cgcgcaaacc cgaagcctcg cgcaatcgct ccagtgagat ttatttattg
    43201 ggcaaaaata aacgctgaca atacagacgg cgtgctttac aatcatttcc gttttacacc
    43261 tcaattatgg agccttgcta agtggggaac acctttaaat caatccttgt ctgggtcgcc
    43321 ttgggtatcg gcctgatggc tgcgttcaac gctttagacg gtaaaaaaga agacaacggg
    43381 caaatcgaat attctcagtt catccaacag gtcaacaacg gcgaagtatc cggcgtcaac
    43441 atcgaaggat ccgtcgtcag cggctacctg attaagggcg agcgcaccga caaaagcacc
    43501 ttcttcacca acgcgccttt ggacgacaac ctgattaaaa cactgctcga caaaaacgtc
    43561 cgtgtaaaag taacgccgga agaaaaaccg agcgcgctgg ctgccctgtt ttacagcctg
    43621 ctgcccgtcc tgctgctgat tggcgcgtgg ttctacttta tgcgtatgca gacgggcggc
    43681 ggcggaaaag gcggcgcatt ctcattcggc aaaagccgcg cccgcctact ggacaaagat
    43741 gccaacaaag ttacctttgc cgatgtcgcc ggctgcgacg aagccaaaga agaagtgcag
    43801 gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa gcctgggcgg gcgcgtgccg
    43861 cgcggcatcc tgctggcggg cagcccgggt acgggtaaga cgcttttggc gaaagcgatt
    43921 gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt ccgactttgt cgaaatgttc
    43981 gtcggtgtcg gtgcaagccg cgtccgcgat atgttcgagc aggcgaagaa aaacgccccc
    44041 tgcatcatct ttatcgacga gattgacgca gtcggccgcc aacgcggcgc aggtttgggc
    44101 ggcggtaatg atgagcgcga gcaaacatta aaccaattgt tggttgaaat ggacggtttt
    44161 gagagcaatc agactgtaat tgtgattgcg gcaaccaacc gccccgacgt actcgatcct
    44221 gcgctgcaac gccccggccg tttcgaccgc caagtggttg tccccctgcc ggacatccgg
    44281 gggcgcgaac agattttgaa cgtccactct aaaaaagtgc ctttggacaa atctgtggat
    44341 ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg cggatttggc gaacttggtc
    44401 aacgaagccg ccctgtttgc cggccgccgc aataaagtca aagtcgatca gagcgatttt
    44461 gaagacgcca aagacaaaat ctatatgggt ccggaacgcc gcagtatggt gatgcacgaa
    44521 gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg cgattgttgc cgaaagcctg
    44581 ccctttaccg accccgtcca caaagtaacc attatgccgc gcggacgtgc gctgggtctg
    44641 acttggcagc ttccggagcg cgaccgcatc agtatgtata aagatcagat gttgagccag
    44701 ctctccatcc tgttcggcgg acggattgcc gaagacatct tcgtcggacg catctccacc
    44761 ggcgcatcaa acgactttga acgcgcaacc caaatggcgc gcgaaatggt aacgcgctac
    44821 ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa acgaaggcga agtcttcttg
    44881 ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa cccagcagga catcgatgcg
    44941 gaaatccgcc ggattttgga cgagcagtat caggttgcct acaaaatcct cgatgaaaac
    45001 cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat gggaaaccat cgaccgcgat
    45061 caggtactgg aaatcatggc gggcaaacaa cccagcccgc ccaaagatta cagccacaac
    45121 ctgcgcgaga atgcggacgc ggcggaagat aacgcgccgc acgctccgac tcgggaagaa
    45181 accgaagcac ctgccccggc agacaccgct tcgacagagt ccgagcagca gcctgaaaac
    45241 aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc cgttttccca tcctgttttc
    45301 agacggcatt ttccgctcaa tgccgtctga aacgccaaag ccgtatcggt ttttgcgaat
    45361 ctgcgatatg gcggtttaaa ttgaaatcag aacggggcaa ggctgtaccg gtttaaaatt
    45421 aaaccgctat aataccgccc ttcgaataca cgcccgcatt ggtgatgccc gtgaaaaaag
    45481 ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat gttcgagctg gtcgataagg
    45541 ttgaggatta cccgaacttt ctgccgtggt acagcaaaac cgaggtcatc ggacgcaacg
    45601 gcaacgaact gaaggcgcgg ctgtttatgg actatatgca cgttcgccaa tcattcgcca
    45661 cgcacaaccg caacattccg ggcagggaaa tccgtatgga actgctcgaa ggtccgttca
    45721 aaaccttacg tggaacgtgg aaatttatcg atttgggcga cgatatgtgc aaaatcgaat
    45781 tcaatttgga atacgatttt tccaatgccg ttttgtccgc cttaatttcc cccgtcttca
    45841 accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga ggcagaccgc cgttatgctt
    45901 gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt tgaaaaccat gcagcttgcc
    45961 gagggaacaa ccgtccgcgc cgccgcactg caaagcggtt tggacggcat atttgaagat
    46021 ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag ccgtcaaaga cgacacgccg
    46081 ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt tgatcgaccc caaagaagcg
    46141 cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc gtctgaagcc ttcagacggc
    46201 attcggcaga aacacggaaa atatcatgtc aaacacaatc aaaatggttg tcggcttggg
    46261 caacccgggc aaagaatacg aacagacccg ccataatgca ggcttttggt tcctcgacga
    46321 actggcttgg aagtggaagg cttcgtttaa agaagaaaaa aaattcttcg gcgaagtcgc
    46381 ccgtgctacc ctgcccgacg gcgatgtctg gctgctcaag ccgaccacgt tcatgaaccg
    46441 ttccggacag gcagttgccg cccttgcgca gttttataaa atcaaacccg aagaaatcct
    46501 cgtcgtccac gacgaactcg acattccctg cggacggatc aaattcaaac tcggcggcgg
    46561 caacggtgga cacaacggct tgaaagacat tcaggcacgg ctcggcacgg ccgactatta
    46621 ccgcctgcgc ctcggtatcg accacccggg cgaccgcaac ctcgtcgtcg gctatgtcct
    46681 gaacaaaccc agtgcggaac accgccaaca gattgatgat gccatcgcca aatccctgca
    46741 ggccataccc gacatcattt ccggcaaatg ggaagaagca acacgtttcc tgcacagcaa
    46801 atgacccaat gccgtctgaa gccctttcag acggcattcc cgccatcagc gagaacccgc
    46861 actatgtccg aactcaaccg gctcatccaa accgaatccg ttgccgtcat tgaagaaact
    46921 ttaaattttc tgctctacga atgcagcata gacgatgccc cctccgccga agaaattgcc
    46981 gtttggcgcg atatgctggc cgcacgcggc ggaaaattcc tgcgcctgtc caaaatctgc
    47041 caaacgtggc tggacgagga ggcggcatga agctgccgcg caaccgcttc agcctgcttt
    47101 ccgcattgtg gtttgccggc ggcatctatt cgctgctctt caaagctgcc gacaccgcgc
    47161 cgccgccgtt tccgcatttc gacaaagcag cacaccttgc cctgtttttc gcacaaatct
    47221 agcttttgac caaagcattc aaaaccggaa aacttcccat cccctaccgc agcctgatgg
    47281 tctttgccct ctgtttcgcc ctcttcagcg aatgcgcgca ggcatggttt accgcaacga
    47341 gaaccggcag tttgggcgat gttcttgccg atatggcagg tacggttctc gcactctttg
    47401 ccgcccgcgc cgccgaccgc ccggactgaa tcggtttatt tcccccaaac aggatgcacc
    47461 tcttcccgat acatcctatt tttcccctta catatcaatc ccatctcata aacaaaaccg
    47521 acaaatcgtt tacaatatat ttacactaca tcagattaca aatatactcg aaccagttgc
    47581 agaatcggcg gttccacaaa ccgtttcgga ttgcacgaca caaaagaaaa accgatttta
    47641 ttgccttaaa ggagtattca tgaacctgca tgcaaaggac aaaacccagc atcccgaaaa
    47701 cgtcgagctg ctcagtgcgc agaagccgat taccgacttt aagggcctgc tgaccaccat
    47761 tatttccgcc gtcgtctgtt tcggcattta ccacatcctg ccttacagcc ccgatgccaa
    47821 taaaggtatc gcgttgctga ttttcgttgc cgcactttgg tttaccgaag ccgtccacat
    47881 taccgttacc gcgctgatga tccctatcct cgccgtcgta ctcggtttcc ccgacatgga
    47941 catcaaaaag gcgatggctg atttttccaa cccgattatc tacatttttt tcggcggttt
    48001 cgcactcgcc accgccctgc atatgcagcg tctcgaccgc aaaatcgccg tcagcctgct
    48061 gcggctgtcg cgcggcagta tgaaggccgc cgtgctgatg ttgttcctcg ttaccgcctt
    48121 tctgtccatg tggatcagca acaccgccac cgccgcgatg atgctgcctc tggcaatggg
    48181 tatgctgagc cacctcgacc gggaaaaaga acgcaaaacc tacgtcttcg tcctgctcgg
    48241 catcgcctat tgcgccagca tcggcggctt gggcacgctc gtcggctcgc cgcccaacct
    48301 gattgccgcc aaagccctaa atctggactt cgtcggctgg atgaagctcg gcctgccgat
    48361 gatgctgttg attctgccct tgatgctgct ctccctgtac gtcatcctca aacctaattt
    48421 gaacgaacgc gtggaaatca aagccgaatc catcccttgg acgctgcacc gcgtgatcgc
    48481 gctgttgatt ttccttgcca cagccgccgc gtggatattc agctccaaaa tcaaaaccgc
    48541 cttcggcatt tccaatcccg acaccgttat cgccctgagt gccgccgtcg ccgtcgtcgt
    48601 cttcggcgtg gcgcaatgga aggaagtcgc ccgcaatacc gactggggcg tgttgatgct
    48661 cttcggcggc ggcatcagcc tgagcacgct gttgaaaaca tccggcgcgt ccgaagcctt
    48721 gggacagcag gttgccgcca ccttttccag cgcgcccgca tttttggtga tactcatcgt
    48781 cgccgccttc attatttttc tgaccgagtt caccagcaac accgcctccg ccgcattgct
    48841 tgtaccgatt ttctccggca tcgctatgca gatggggctg cccgaacaag tcttggtatt
    48901 cgtcatcggc atcggcgcat cttgtgcctt catgctgccg gttgccacac cgcctaacgc
    48961 gattgtgttc ggcacaggct taatcaagca acgcgaaatg atgaatgtcg gcatactgct
    49021 gaacatcctc tgcgtagtat tggttgctct gtgggcttat gctgtactga tgtaaaccat
    49081 cgacctaaac aacaagaccg tctgaaagaa tatttttcag acggccttga agtttgtcgt
    49141 tcagacacaa tttgtcgaat cattcaaaac cggattctaa cgaaaggaaa cccatgatta
    49201 tcctgcacac caacaaaggc gacatcaaaa tcgaactcga tttcgacaaa gcccccgtta
    49261 ccgccaaaaa cttcgagcaa tacgtcaaag acggcttcta cgacggcgta atcttccacc
    49321 gcgtcatcaa aggcttcatg attcaaggcg gcggcatgga tgaaaacatg aacgaaaaag
    49381 aaacccgcga tccgattcaa aacgaagcgt ccaacggcct gcccaacgat aaatacacca
    49441 tcgccatggc acgcacttcc gacccccatt ccgccagcgc gcaattcttc atcaacactg
    49501 ccgacaacgc tttcctgaac ttccgttcta aagagctgta cggcaaaacc gtcgtccaag
    49561 actggggcta tgcagtattc ggtaaagtcg ttgacggttt tgacgttgtc gatgccatcg
    49621 aaggcgtatc caccaaacgc cacggctacc atgacgacgt accgagcgaa cctgtcgtca
    49681 tcattaaagc cgaagcggta taaaccggca atccgaaagc agcctgcaca aaggctgctt
    49741 tttttgtttc aggcggcata aaatccaagc acatatgccg tctgaacacc tcccgcaagc
    49801 cttcggacaa tgttgcaacg tgttaaaata gcctcctttc ctgaaaccac aacaaggaaa
    49861 ccatcatggc aaatattgcc cgcgacatct tcaaagccta cgacatccga ggcattgtcg
    49921 gcaaaaccct gactgacgat gccgcctatc tcatcggcag ggccatcgcc gccaaagccg
    49981 ccgaaaaagg catgacccgc atcgcgctcg gacgcgacgg acgcttgagc ggtcccgaac
    50041 tgatggagca catccaacgc ggctttaccg acagcggcat cggcgtactc aacgtcggca
    50101 tggtcgccac tcctatgctc tattttgccg ccatcaacga atgcggcggc agcggcgtga
    50161 tgattaccgg cagccacaat ccgcctgatt acaacggttt caaaatgatg ctcggcggcg
    50221 acacgctcgc aggcgaagcc attcaagaac ttttagctat tgttgagaaa gatggttttg
    50281 ttgccgccga caaacaaggc aacgtaaccg aaaaagacat ctccggcgaa taccacaacc
    50341 acatcgtcgg ccacatcaaa ctcaaacgcc ccatgaaaat tgccatcgac gcgggcaacg
    50401 gcgtgggcgg cgcgtttgcc ggcaaactct ataaaggctt gggcaacgaa gtaaccgagc
    50461 ttttctgcga cgtggacggc actttcccca accaccatcc cgacccatcc aaaccgaaaa
    50521 acctgcaaga tttgattgcc gcgctgaaaa acggtgatgc cgaaatcggc ctggcgtttg
    50581 acggcgatgc cgaccgcttg ggtgtggtca ccaaagacgg caacattatt tatcccgacc
    50641 gccaactgat gctgttcgcc caagacgttt tgaaccgcaa tcccggcgcg aaagtcattt
    50701 tcgacgtgaa gtccacccgc ctgcttgcgc cttggattaa agaacacggc ggcgaagcca
    50761 taatggaaaa aaccggccac agctttatca aatccgccat gaaaaaaacc ggcgcgctgg
    50821 ttgccggcga aatgagcgga cacatcttct tcaaagaacg ctggttcggc ttcgacgacg
    50881 gtctgtacgc cggcgcacgc ctcttggaaa tcctgtctgc ctccgataat ccgtccgaag
    50941 tgttaaacaa cctgccgcaa agcatttcca cgcccgaact caacatcgcc ctgcccgaag
    51001 gcagcaacgg ccatcaggtt atcgacgaac tcgccgccaa agccgaattt gaaggcgcaa
    51061 ccgaaatcat caccatcgac ggtctgcgcg ttgaatttcc cgacggcttc ggtctgatgc
    51121 gtgcttccaa taccacgccg attctggtgt tgcgttttga ggcggatacg caagcagcca
    51181 tcgagcggat tcaaaaccaa ttcaaagccg tcatcgaaag caatccgaat ctaatctggc
    51241 ctctgtaaac acaggaaaaa tgcttaatca aatcttggta gattggattt ggcttaaaaa
    51301 tctccgcgtc cgttcgtgtt tggggtggtc gaacaactct ttcgtgctgc cctgctctac
    51361 gataacgccg ccgtccatca cgacaacggt cgtggcaact tccagcgcga acttgatttc
    51421 gtgggtaacg acgaccatcg tccaaccttc ccgcgccaat tccttcatgg cgttcaacac
    51481 gtcttgcacc aactcggggt caagcgcgga agtgggttcg tcaaacaaca tcagctcggg
    51541 ctgaatcgcc aatgctcggg caatgccgac gcgctgctgc tgaccgccgg aaagctggta
    51601 gggatacaaa tccactttgt cgcccaagcc gactttttcc agcagtttca gagcctcttc
    51661 gcgcgcttgg gcggcaggct tgccctgtac ggcaaccggt ccttccatca cgttttccaa
    51721 ggcggttttg tgcggaaaga ggttgtattg ttgaaacacc atgcctgatt tgcggcgcag
    51781 tgccaaaata tcgtgtttgc ttggtttttt agaaaaatcg attttcagcg gtcgctcgtt
    51841 gtcgaactcg atttgtccgt cttcgggcat ttccaacgcg tttaggcatc gcagaaacgt
    51901 cgttttgcct gagccggaag gcccgaggat gacgaccacc tgccctttgc acacatccaa
    51961 attgatgccg cgcaaaatgg tatttttgcc gaaggtctta tggatattgc ggattttaat
    52021 catgacaact ccttatttgg cgacgtagcg gtcgaaacgt ttttccaaac gcgcctgaat
    52081 caggaacagc actttacaaa aacaccagta aaccaaagcg gcttcgatat agacgggcaa
    52141 aaagtcataa gtgcggtttg ccgtttcctg cgcgacgcgg aataattccg ttaccgtcac
    52201 gactgccgcg agcgaggtgt ttttaaacaa accgataaac tcgttgctca aaggcggcac
    52261 ggcaacgcgg aatgcctgcg gcgcaacgat gcggcggaac gtctgcatat aggtcatgcc
    52321 gatggagaaa cctgcttccc attggccttt cggtacggac aaaattgccg cgcgtatggt
    52381 ttcggaagca tatgcgccga cattgagcga aaagccgatg atggcggcag gaatcgggtc
    52441 gatatagatg ccgacggaag gcagcccgta aaacacaatc acaagctgaa ccaacagcgg
    52501 cgtaccgcga atgacggaaa tataaaattc caccaatttc agcaggattt tccgcacgat
    52561 gccgccggcg ggcataatcc gcaccaaagc cacggctacc gcaatcatca taccgataac
    52621 gaaagaagct gccgccaaag gcagagagac cgcgaagccg gctttgacca taggcaaaaa
    52681 cgcgctgaca atcatatcgg cgcgtgtttc cgtcataaac ggcagcgaag caaggaaatt
    52741 attgaacact gatgtctttt ccgaagaatt gttcgcccag ttttttcagc gtaccgtcgg
    52801 ctttcagctc gttgattgcc gtactgaatt tcgccacggc ttcgtcattg cccttgttga
    52861 caatcaggcc ggaaccgact ttttcatcgg caggtgcgga ccaaacgatt ttcacgcccg
    52921 cattcgggtt tttcttcaga tagtccaaaa ccgccaattc gtcgttcagg gttgcatcgg
    52981 cacgtttttg ttcaatcagg gtcagcgatt gcgccaaacc gtccactgcc accaaatctg
    53041 cgcctgcagc tttggctttt tcgccgtagt tgctggtcag ggattgtgcg gttttcacgc
    53101 ctttgatgtc gtcgatagat ttgatgttgc tgtcgttacg ggcaaccaat acggcaccgc
    53161 tccagctgta aggatcggat ttgtcgaatg tcgcttggcg ttcggggctg gtcagaccga
    53221 cttggtttgc caccacgtcg aaacgccccg ccttcaaacc cgccatcatc gaatcccatt
    53281 gcgtttcttt aaactcgact ttcacgccca gtttttccgc cacggcgcgg gttacttcca
    53341 catcgtaacc ggtcagtttg ccgtccttgt cgtggtaggt aaacggtgcg taagtgcctt
    53401 ccgtgccgac ggtaaccgtg cctttattgt tgatgcgctc gattaaagaa ccggaaactg
    53461 ccgattgtgc aggcgcggaa gatgctccgc tgccgctttc cgaaccgccg caggccgcca
    53521 aaaccaatgc ggcaatgccg ccgagtacga attttttcaa catcattctc tcctgtaaaa
    53581 agtgtgcaag tgcggcattc taagtgaaat tgcggcaaac acaaagaata ctttttttat
    53641 atggttattc caaatcggaa tggaaaagat atgccgaagc cgccggaagc ggaaataaag
    53701 ggggcgggaa aggcggcggg cggcacgaaa ccgaaaagcg gcatcaggta tgcaaacggc
    53761 ggattcacgc acgccgttcc ggcaaaaatc cgcctgccga ttcggtgcta taatccatcc
    53821 gaaacagatg acaccaaaaa ggaacatctc atgaacacgc cccaatccgc catcattccc
    53881 gaccacgccc aagccggcat ctttatcgaa gccgacttcg ccgccaaccg cctcaacgat
    53941 attaaagccg cctgccgcgc ttcgctcgac gcgttatccg ccttgaaaac ccgctttccg
    54001 gacgatattt tgggtctgac catcgccttc ggcagcaaag cctgggcaac attcggacat
    54061 acggacgaag gcagtgaaat caaacccttc tccgaaatgg gcaacgggct tgcgccgtcc
    54121 acgcagcacg atatgtccat ccacatccaa tctttccgcc aaaacgccgc ctacgcgctt
    54181 gcccaatccg ttttgggcgc gttcggcgac agcatatgcg tcgcgtccga agaacacggt
    54241 ttgcgcctgt atcaggatcg cgggctggac ggtttcgtcg acggcacgga aaacccgcag
    54301 ggcgatgaaa atgtccgcga agtcgccatc atccccgaag gaaaacccga tgcgggcggc
    54361 agctatgtcc tgctgcaaaa atacctgcac gatttgaaaa aatgggatgc cgtccccgtc
    54421 gccgaacagg aggcctcggt cggacgcggc aaggaaaccg acgacgaatt cggccacgat
    54481 gtccgcctgc ccgattcgca cctcggccgc gtcaacctga aagaaaacgg agtcggcctg
    54541 aaaatcgtcc gccgcagcct gcccttcggc aaaatcagcg gcgaacacgg tttgatgttt
    54601 accgcatact gccgcacgct gcacaatatc gaagcgcaat tattgagtat gttcggcgac
    54661 acggatggca aaaccgacct gctgctccgg cacctctccg ccgccgtttc gggcggatac
    54721 tactacgcgc cgtctgtcga acgcctgcaa aacctctaag ccagacgatg ccgtctgaaa
    54781 ccatttttca gacggcattt t
//
LOCUS       DAWKOD010000002        40286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000002 DAWKOD010000000
VERSION     DAWKOD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.002"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WJK73_000056"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WJK73_000056"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ7556606.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     IYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLASKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANCFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3082..3870)
                     /locus_tag="WJK73_000057"
     CDS             complement(3082..3870)
                     /locus_tag="WJK73_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ7556607.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIVSMF"
     gene            complement(4031..5185)
                     /gene="prpC"
                     /locus_tag="WJK73_000058"
     CDS             complement(4031..5185)
                     /gene="prpC"
                     /locus_tag="WJK73_000058"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ7556608.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5271..6146)
                     /gene="prpB"
                     /locus_tag="WJK73_000059"
     CDS             complement(5271..6146)
                     /gene="prpB"
                     /locus_tag="WJK73_000059"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ7556609.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6772..8082)
                     /locus_tag="WJK73_000060"
     CDS             complement(6772..8082)
                     /locus_tag="WJK73_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ7556610.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8221..9399)
                     /gene="ftsZ"
                     /locus_tag="WJK73_000061"
     CDS             complement(8221..9399)
                     /gene="ftsZ"
                     /locus_tag="WJK73_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ7556611.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9518..10762)
                     /gene="ftsA"
                     /locus_tag="WJK73_000062"
     CDS             complement(9518..10762)
                     /gene="ftsA"
                     /locus_tag="WJK73_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ7556612.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQTPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10848..11576)
                     /locus_tag="WJK73_000063"
     CDS             complement(10848..11576)
                     /locus_tag="WJK73_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ7556613.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11566..12480)
                     /locus_tag="WJK73_000064"
     CDS             complement(11566..12480)
                     /locus_tag="WJK73_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ7556614.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHFQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            complement(12593..14002)
                     /locus_tag="WJK73_000065"
     CDS             complement(12593..14002)
                     /locus_tag="WJK73_000065"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ7556615.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MCFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14156..15223)
                     /gene="murG"
                     /locus_tag="WJK73_000066"
     CDS             complement(14156..15223)
                     /gene="murG"
                     /locus_tag="WJK73_000066"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ7556616.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15227..16552)
                     /locus_tag="WJK73_000067"
     CDS             complement(15227..16552)
                     /locus_tag="WJK73_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ7556617.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16678..18015)
                     /locus_tag="WJK73_000068"
     CDS             complement(16678..18015)
                     /locus_tag="WJK73_000068"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ7556618.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDTFCCAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPRNELLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRHDLDGCGLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(18099..18371)
                     /locus_tag="WJK73_000069"
     CDS             complement(18099..18371)
                     /locus_tag="WJK73_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7556619.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     NNLGVMYAERRGVRQDRALAQEWLGKACQNGCQDSCDNDQRLKAGY"
     gene            complement(18486..19568)
                     /locus_tag="WJK73_000070"
     CDS             complement(18486..19568)
                     /locus_tag="WJK73_000070"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ7556620.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19662..19838)
                     /locus_tag="WJK73_000071"
     CDS             complement(19662..19838)
                     /locus_tag="WJK73_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556621.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(19841..21199)
                     /locus_tag="WJK73_000072"
     CDS             complement(19841..21199)
                     /locus_tag="WJK73_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ7556622.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21324..22970)
                     /locus_tag="WJK73_000073"
     CDS             complement(21324..22970)
                     /locus_tag="WJK73_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ7556623.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVPGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(22993..24471)
                     /locus_tag="WJK73_000074"
     CDS             complement(22993..24471)
                     /locus_tag="WJK73_000074"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ7556624.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24496..26241)
                     /locus_tag="WJK73_000075"
     CDS             complement(24496..26241)
                     /locus_tag="WJK73_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ7556625.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(26302..26565)
                     /gene="ftsL"
                     /locus_tag="WJK73_000076"
     CDS             complement(26302..26565)
                     /gene="ftsL"
                     /locus_tag="WJK73_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ7556626.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(26562..27548)
                     /gene="rsmH"
                     /locus_tag="WJK73_000077"
     CDS             complement(26562..27548)
                     /gene="rsmH"
                     /locus_tag="WJK73_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ7556627.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(27545..28000)
                     /gene="mraZ"
                     /locus_tag="WJK73_000078"
     CDS             complement(27545..28000)
                     /gene="mraZ"
                     /locus_tag="WJK73_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ7556628.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            28279..29496
                     /locus_tag="WJK73_000079"
     CDS             28279..29496
                     /locus_tag="WJK73_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7556629.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            29628..29704
                     /locus_tag="WJK73_000080"
     tRNA            29628..29704
                     /locus_tag="WJK73_000080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29662..29664,aa:Met,seq:cat)
     gene            29712..29787
                     /locus_tag="WJK73_000081"
     tRNA            29712..29787
                     /locus_tag="WJK73_000081"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29745..29747,aa:Ala,seq:ggc)
     gene            complement(29919..30023)
                     /locus_tag="WJK73_000082"
                     /pseudo
     CDS             complement(29919..30023)
                     /locus_tag="WJK73_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30079..30900)
                     /locus_tag="WJK73_000083"
     CDS             complement(30079..30900)
                     /locus_tag="WJK73_000083"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ7556630.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(30933..31577)
                     /locus_tag="WJK73_000084"
     CDS             complement(30933..31577)
                     /locus_tag="WJK73_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7556631.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(31580..32425)
                     /gene="ftsN"
                     /locus_tag="WJK73_000085"
     CDS             complement(31580..32425)
                     /gene="ftsN"
                     /locus_tag="WJK73_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7556632.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(32531..34027)
                     /locus_tag="WJK73_000086"
     CDS             complement(32531..34027)
                     /locus_tag="WJK73_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ7556633.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(34041..34361)
                     /locus_tag="WJK73_000087"
     CDS             complement(34041..34361)
                     /locus_tag="WJK73_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ7556634.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34844..36370
                     /gene="putP"
                     /locus_tag="WJK73_000088"
     CDS             34844..36370
                     /gene="putP"
                     /locus_tag="WJK73_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ7556635.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKAYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            complement(36460..36627)
                     /locus_tag="WJK73_000089"
     CDS             complement(36460..36627)
                     /locus_tag="WJK73_000089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556636.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            36619..40224
                     /gene="putA"
                     /locus_tag="WJK73_000090"
     CDS             36619..40224
                     /gene="putA"
                     /locus_tag="WJK73_000090"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ7556637.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         9519 a        11418 c         9990 g         9359 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcggc aggtaacggg aacttcgacg gtttctccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt gcgttcgccg accacgtcgt aggtttccgt accgtccagt
      601 tgcagggtat ggcggttggt gtcgggtttg aactgcagcg gcaacacgcc catgccgata
      661 aggttggtgc ggtggatacg ctcgaagcct tcggcaacaa tcgcttctac gccggcgagg
      721 cgtacgcctt ttgcagccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tggccttcgg gttcgacgcg tgccagcgaa ccttggcgta cgctgccatc ttcgtttctc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagtgcg
     1141 ccttcccagt aaggtgggcg gcggatatag gtggacatcg gacgccagtc gtacagcgga
     1201 ctaggtgctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcggc aacgacggca tcgatttctt catcggcagg ccaaatgtct
     1321 ttcaggcgga tttccttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg
     1381 atgctgcctg ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc
     1441 gcatacggat ggacacggcc gtcgaagttg cggttgcctg ataatacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctggatt ttcggatcca gcgcgccact catgccgttg
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcagcagg
     1621 cctgcttctt tcaaataaat ttccgccact ttcgaaccgg gggcaaacga ggttttgacc
     1681 cacggtttgc gtttcagccc gaagcagttg gcgttgcgcg ccaagagcgc ggcggcaaca
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgcgccg
     1801 tcgggcattt ggccgtctga aggctcttcg taaggtttag ccaagccttt gctggccaaa
     1861 tcggcggtcg caaaacgcgc gtgcgggttg ctcgggcctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgcggataa acggcggttt tcaaggcatc tgcccataag
     1981 cctgcggttt tggcgtagat ttctaccaat ttcacttgcg cgtcgtcgcg accggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactca
     2101 ggggtcatgt tggaaatggt ggcacggtcg ccgatggaca ggcttcttgc accttcgccg
     2161 aagaattcga caaatgcgcc aaccacgcgc tctttacgca agaattcggt cagtgccaac
     2221 acaatatccg tcgccgtaat gcccgccttc cgtttgccgg tcagctcaac gccgacaata
     2281 tcaggcagac gcatcatgga tgcacggccc aacattacag tttcggcttc caaaccgccc
     2341 acgcctacag aaatcacgcc caatgaatcg acgtgcggcg tatgtgaatc tgtaccgaca
     2401 caggtatcgg ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaaagcggtt
     2521 tttgtccagt tgatgaagtg gaaacggtct tcgttacgtc tgtcttcgat ttcgcggttt
     2581 ttgcggaacg catcggggtc gtagccgccg cattcaacgg ccagagagtg gtcgacgatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca
     2701 tcgcgcagac ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accaaggaaa gtcgatttcc tgttttccct caatcagctg acccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctggggat ctgtttttct tgtggtttga ggtttcagcc gacgtttttg
     3061 gaaggggtcg tctgaaagag tttaaaacat cgaaacaatc atctgataca gcggatttct
     3121 ctcgtctgtc aacaatttca cagccatcga aatgctgatg acaatcagca gcggcttaat
     3181 cagcttcgaa ccgaagcgga cggcaaatct cgcacctaaa ttcgcaccga caaacgcacc
     3241 gaccgccatc gttgccgcaa tcgggaaaat aatcgaaccg tgcagcagga ataccgatag
     3301 cgaaccaaga ttgcaggcaa cgttcgccaa tttggtgtaa gacatcgcgt tcaacagctt
     3361 gcagccgagc aaaacaataa aggcaatcag aaaaaacgag ccgacacccg gtccgaacac
     3421 accgtcgtaa aaacccaaaa gtggtgcaac cgtcagaccg aacagaaaaa aagacattct
     3481 ggctttgcct tccttactgc cgtcgagctt gggcgaaaac acaaaataca gcgcgacaaa
     3541 tatcaacaaa accggcacga ccgccagcag aatatctttg gaaaccaagc tgaccgataa
     3601 tgcaccgacc acgccgcctg caaacgatgc tgccgcaatc gggagacctt tcttccaatc
     3661 aatcaaacct ttgcgtgcaa aagaaaccgt agccgaaaac gtagcagcgg ctgcttgcag
     3721 cttgttggtg gcaattgccg acacgggagg aatacctgcc aacaagagtg caggcagcgt
     3781 aatcaaacca cccccacccg caatcgcatc gataaatccg gcaatcatcg caaccaaacc
     3841 caaagcgagt attatgtata aatcttccat gtttcttatc ctgttacttg caccaataca
     3901 ggattatctt cttctattag gttaaatttt taaacttatt tcagacaacc tttccaatca
     3961 ggcaaggtcg tctgaaatcc tcagctttgc ataccgaaat taaagaacaa actgctattg
     4021 cattcttcaa ttatcgttct tcaatctcca caaacgccaa atcttcaggg cctgtgtagt
     4081 ttgcgctcgg acggatgatt ttgccgtctt tgcgttgctc aagaacgtgt gcgctccaac
     4141 cggttgtacg ggaaattacg aacagcggtg tgaacatagc ggtcggtacg cccaattttt
     4201 ggtaggaaac ggcagagaac cagtccagat tcgggaacat ttttttctct tcccacatca
     4261 cgctttccaa acgttcggca atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac
     4321 cgcgtgccac ttctttaatg acaacgttgc gagggtcgga aatggtgtac actggatgac
     4381 cgaaaccgat cacgatttct ttgcggccga tgcgttcgcg gatgtctgct tcggcttcgt
     4441 cggcattgcg gtagcgtttt tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg
     4501 gacctttcaa cgcgccgatt gctccggtaa tgctggagta catatcagag cctgtaccgg
     4561 caatcacgcg ggcggtgaag gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg
     4621 aaacgtgcat ggctttgatg tgggattcgc ttgggcgttt gccgtgcaac agttgcagga
     4681 aatgaccgcc gatggtctct tcgtcgcttt caacctcaat gcgtttgccg ttgtgcgaat
     4741 attgatacca gtacaagagg atgctgccga ggctggcgat cagtttgtcg gcgatgtcgc
     4801 gcgcttcact ttccggatgg ctttcacgtt caggatgaac gcagcccagc atggatacgc
     4861 cggtacgcat tacgtccatc ggatgggtat gtgcaggcag gctttccaaa actttaatca
     4921 cacggatagg caggccgcgc atggatttga gcttggtttt ataagcggcc agctcgaatt
     4981 tgttgggcag atgaccgtga atcagcaggt gggcgacttc ttcaaactcg catttttgtg
     5041 ccaaatccaa gatgtcgtaa ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac
     5101 acaaagcggt attaccggcc gcaacgcctg aaagcgcaac ggatttctta ggtttgaagg
     5161 tcggggtttg agtagtttca gtcatggtat ttctcctttg tgtttttatg ggtttcgggt
     5221 tttcagacga ccgatgcgga tttgttgaaa gaccgtctga aagcggtaaa tcatttttga
     5281 aacaatttat ccagtttttg ctcgaaggca tgatagttca gatgctcgta cagctcggca
     5341 cgggtttgca tactgtccac caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa
     5401 acattcagag cggctttgct tgctgcacgg aacgatgaca gcggatacag caccagcgac
     5461 acgccgtttt cagccagctc gctttgggta taaagcggag tggaaccaaa ctcggtaatg
     5521 ttcgccaaca cgggcacttt caccgcatct gcaaattggc ggtacatgtt caaatcggtc
     5581 atggcttcag ggaaaatcat gtccgcaccg gcttcgacac aagcttgggc gcgttcgata
     5641 gcggcatcca aaccttctac cgccagcgca tcggtacgcg ccataatcac gaagttctca
     5701 tcaacgcgcg catctacggc agctttgata cggtcgacca tttcatcttt agatacaatg
     5761 gctttgttcg gacggtggcc gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct
     5821 gcaacaccgg cgcgttcaaa gttgcgaatg gtacgggcaa tattgaatgc accgccccaa
     5881 cccacatcga tgtccaccag cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg
     5941 atcagcacat cttccattgt ggtaatgccc aaatcaggga taccgcaaga acaggctgcc
     6001 acgccgccgc cggacagata gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa
     6061 taagcattga cgcaaccggc gacggcaagc ggattcgatt ctttcacggc ttggcggaaa
     6121 cgtgctccgg cagagtgttg actcatcata tttctccttt atagactttt tttcagtatg
     6181 gacaggcttc catcacattc ggacggcaaa acacagccat ccgacgcgtc gggcatccca
     6241 atccaatcat taaaaatata tgggaaaaat tatcttattg atatttaaaa cgaatcaaag
     6301 aaaaacagca gaccgttcgg aattatgcgg caaaaccgca gacaagaaga aaacaagggg
     6361 attattcaga aaaggggaaa acatctgaat tggtttcata gtaatgttcc tttgtagtgt
     6421 tatgtagttt tatttttcga taatctaaaa cacaagactg aaaatgtcaa acatttttaa
     6481 ggagactcat tacataattt taatatttta ttttaattcc atgattaaac tagattaatt
     6541 ttgaattatt tgtttcaata aaattgtcag gaaatcaaga aaaattttcg ataatcaaaa
     6601 tcctgaaaac ttataaaata ttttatacat aaaaacaatg tattattttt aagcaatccg
     6661 atgtatttta ttatgtattt gatttttata taatttaata aaaattaaat atatcggcaa
     6721 acaaaatgcc gtctgaaaac ctttcagacg gcatttttaa tatcgaatca atcagcccaa
     6781 ataccagaat ttcagtgccc acaatacggc aacaatccat accataggcg gaacgtcttt
     6841 ggtgcggcgg cataaaagtt taaccacggc atagctgatg aagccgaagg cgatgccgtc
     6901 tgcaatcgaa taggtaaacg gcatgaagac aatggtcagg aatgcgggtg cggcttccgt
     6961 catatcgtcc caatcaatat ccctcgcact gcggagcatc tgcgtgccga cataaagcag
     7021 ggcgggcgcg gtggcaaaag cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc
     7081 gagcatcaat acgccgactg taaccgccgt caggccggtc cgtccgcctg ccgatacgcc
     7141 cgccgcgctt tccacataag gcgtggtgga agaagtaccc aaagccgcac ctgccacaat
     7201 ggcggtagag tctgcaagca gtgcgcgttt caggcggggc agcttaccgt ccaccagcag
     7261 cccggcacgg tgggatatgc cgaccagcgt tccggtactg tcaaatagat cgaccaagaa
     7321 gaagacgaaa atcacactga ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat
     7381 aaaagtcggc gcaatgctcg gtacttcgcc gatgatgccg tgaaattcat tcaaacccat
     7441 caggctggca atgacggtaa tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa
     7501 atgtcccaat acgaccacca tagcaaaacc gaacagtgcc aacaacgcgg acggctgatg
     7561 aatatcgccc aaaccgacca aggttgccgg attggcaacg ataatgcctg cgcctttcag
     7621 ggaaatcagt gccaaaaaca aaccgatacc ggcagcaatc gacattttca aacccatagg
     7681 cagtgcgttg accagcattt ccctgacttt aaaaaagctg aacaggataa aaatcagacc
     7741 ggagatgaac accgcaccca acgcaacctg ccaaggcacg cccataccct taacgacggc
     7801 aaaggtgaaa taggcattca gccccatccc cggtgcgagt gcaatcggat agttgccgac
     7861 aaaacccata acaaaacagc cgatggcaga cgcgatacag gtagcgacga ataccgcccc
     7921 catatccatg ccggtctcgc ccaaaatcag agggttgacg ataacgatgt agcacatcgt
     7981 caaaaaagtt gtcaaacccg ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag
     8041 cttaaaaatc ccgtccaaca gtgtttgttt tgaagtgtcc atatctgaaa gtctgtatca
     8101 agtggaaaaa tctgaatccg ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac
     8161 atacctccca aaccggacaa accgattcgg gaggcagcag gtttacgggc aaacagcaca
     8221 ttatttgtct gaattgtgtt gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac
     8281 ggactgattg tcgaaatccg cagcggtaag gttcatcgtg cggataccgc gattggtgcg
     8341 gatcatacct tcgacattgt ggctttgctc ctgtttggac ggagcaaccg cttctacctc
     8401 ccttgccgga acaaaatcga ccgcgccttt ttctttcaga ccggtagcga taatggtaat
     8461 ccggatggca tcttcgctca tggtctcgtc ttcagccgca ccgaatttgc attccaaatc
     8521 gggatgcgcg ctttggttga cgattttcat gacttcggac aactcggaca ttttcaagca
     8581 acccggagca gtcgtaatat tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc
     8641 cagcagcgga ctggaaatgg cctggtcggt cgccatacgc gcacggtcga taccttgggc
     8701 ataacccgaa cccatcatag cgataccgcg gttgctcatc acggttttca cgtcggcaaa
     8761 gtcgaggttg atgatttcgc tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc
     8821 gcgcaataca ttgtcggcgg cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc
     8881 agtcatcagt ttgtcgttcg ggatgatgat cagcgaatcg acgtgttctt tcaactgttc
     8941 taaccctgcc tgtgcgacat ggacgcgctt accttcatat gcgaacggtc gggtaaccac
     9001 ggcaacggtc agaatgccca aagacttggc aatctcagca acaaccggcg cggaaccggt
     9061 accggtaccg ccgcccatac cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc
     9121 ttcaatggct tcccggtctt cctgggctgc cgcacggccg atatcgggat tcgcgcccgc
     9181 gcccaaaccg cgtgtcagat tcgtacccaa ctggattctc ttcgccgcat ggttttttgc
     9241 cagagactgc gcatccgtat tggcactgat aaactccaca ccgcgcacat tgttggcaac
     9301 catgttattg attgcattgc aaccgccgcc gcccaagccg attactttaa tcaccgcagg
     9361 gctgactgcc gattctgcca cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc
     9421 attcagagga cggtttaaat aaattattat tcattatata agaagtatct tgctgctggc
     9481 aaaatacttc tcacctgtca aacggcaatc cacctgttca gaagctgttt tcaatccacc
     9541 gtttcaatct tgccaacaaa ccgccgcccc cttccctctc ttgcactgca ccgttttccg
     9601 gctgcggcaa gtttccttcc agcttgcatg ctgcatgaag cagcccgata gcggtagaaa
     9661 aacgcggtgt gcggacgcgg tcggacaaac cgcccatttc ttggggtgca ccggtgcgta
     9721 caggcaaatc gaagattttt tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc
     9781 cggtcagaac gatacccgca ttcagcactt ctttggggaa acccgatttt tgcagctcgc
     9841 ccagcactac gccaaaaatc tcctgaatcc gtgcactgat gattgctgcc agaaccttac
     9901 tggaaacctg acgcgatgtc cggtcaccca cgcccggaac ttcaatcatc tcacccaagc
     9961 cttccgtatc gcatgatgcc acgccataat ggattttaat gtactcggcg gcatcgagag
    10021 gtgttctcaa cgatttggac aaatctttgg taatcagatt accaccggcc ggaatgacgg
    10081 acgtatggcg gatggcaccg ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt
    10141 cgatgacgca tacgccgagg tctttttcat cttcagtcag caccgcctgc ccgcttgcca
    10201 acggctgaag catgatctga tcgcttttca aaccgcaccg ctcgatacat ttttggacat
    10261 tctgcactgc cgtacttgca ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc
    10321 tcataccgat gggctccctc acgccaagtt gggtgtcgat aatgtagtct tgaaccacgg
    10381 catcgagaat tttttgatcg ggcgggatat tgattgcctt tgccgtttca atggcgcggt
    10441 cgatgtctgc ctgcgtgact tccccgtctt taattttaac cacaccttgc gaattgagac
    10501 tgcggatgtg gttgcctgcg atacctgtgg taacgtgggt aattttggta tccgccatca
    10561 gctcggcatc attgaccgcc tgcctgatgg cttggacggt ggcatcgata ttggttacca
    10621 tgcccgcgcg caagccccgt gaaggagtct gccccaaacc gacgatgttg attttgtcgt
    10681 catcttgaac ttccccgatc agtgcgagga ctttagacgt accgatatcc agtacgctga
    10741 tgtatctttg ctgctgttcc attgttcgtc tgctcttaaa actgattgaa atttgcgtcg
    10801 caccatttca gacggcacgg ccgtaatctg tccgatacct gttcccacta ttcttcggat
    10861 tctttttcgg gtaaaccgtc ggaagcatag cggactgaaa atccgtcctt atacctcata
    10921 tccacatagg ataaccgatt tttattttta cgcaacagat gctgccacgc ttcggtaaaa
    10981 agccggaggc gtttcatctc gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa
    11041 acgacaatcc acgccgaacg tgccgtatag gtcatctctt tgatgcccaa accctgtttt
    11101 gccaaaacag tcgaaaattc gtcataacgg cggagcattt cggcagacgt tccttccgcg
    11161 cctctgaata ccggcattcc gggtctgtcc aagcgggctt caaaaacatt gccttcgccg
    11221 tccaccaagg catggtcgcc ccaacgcgcg accggcttgc gctcggtcag gacgacctca
    11281 accgtgtcgg gaaaacggcg gcgcaccatg accgacgcaa tccacggata ccggcggtag
    11341 gcctcctgtg cgccattgat gtccgtcctc aaaatattcc catggatgta ttctttcgcc
    11401 aaactgccca atgctttctt atcggaataa accaggttgc ccttcagcga cacctgcttg
    11461 acgggcagat gattcgaatt gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc
    11521 atcatgacaa gcagccagcg cgtcagccgt tccatcgctt cggcattatc ccacatgtgc
    11581 ggtcttcaaa atttcaatac ataaatcggc aaaacccacg cccataacgg cagcggattt
    11641 cggtactaaa ctatggccgg tcataccggg cagggtgttg atttccaaca gatagagttt
    11701 gccgtcggta tctttgagga aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc
    11761 gccgcgaacc gccagttcgc gcatcaggct ttcttcggct tcggtcagat cttccgaagg
    11821 acattgataa atggtgtcgt tgcggttgta cttggcttcg tagtcataaa actcggtcgc
    11881 ggggatgatg tgtatgccgg gcaggccttt gccgttcaac acagggcagg aatattcgcc
    11941 gccgccgata aaccgttcgg caatgatttc gccctgaaag tgtttcaatt cttcgtaaac
    12001 gcttttcaga cggccttttc ctttgacttt taccacgcct acgctgctgc cttcggccgc
    12061 cggtttcaca aacatcggca ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc
    12121 gtgcaggacg gcgaactcgg gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg
    12181 gtatttgtcc atgccgatgg cggatgcggc gacaccgctg ccggtatagg gaatgcccaa
    12241 cagttccaat gcaccctgaa cagccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa
    12301 tgccgtctga aaaccttgtg ccttcaattc agacaatggg gtttccttgg gatcgaaggc
    12361 gtatgcgtct atgcctttgc tttttaaagc attcaaaatg gcggtgccgc tgtccagcga
    12421 gatttctcgt tcgctggaaa aaccgcccat caatacggcc actttgccaa aattctgcat
    12481 tgtttttgtt ctttcctgat tgctttatgc ttgttgccag aggtcgtctg aaacctgatt
    12541 tgcggtttca gacgaccttt atatgatgtt ccgtctgtca ggcgggtgtg cctcaaatct
    12601 gtttcgacaa ttccagcagc gcggcgggga cgcggttgat gcttcccgca cccatattca
    12661 acacgatgtc gccgtcctgc aaaacgttca acagcatttc gggcagatcg gcaacgtttt
    12721 cgcagtaaat cggctcgagt ttgcccaaca cgcggatggc gcgggcaaga gcgcgggaat
    12781 cggcggcggc aatcggctct tcaccggcgg cataaacttc ggtcagcacc agcgcgtcaa
    12841 cggtattgag gactttggta aagtcttcaa acaaatcgcg cgtgcgggta tagcggtgcg
    12901 gctggaaggc gagtaccaaa cgtttttccg gatacgcgcc gcgtgcggcg gaaagggtcg
    12961 ccgccatttc gacggggtgg tgtccgtagt cgtccaccaa gagcgcggtt ccaccgtttg
    13021 gcaacttgat gtcgccgtat ttttggaagc ggcggccgac accttcaaag ccgagcaagc
    13081 ctttttggat cgcttcaacc gatgcgccga cttccagcgc cacgccgatg gctgccaatg
    13141 cgttcagcac gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac ccctgctcat
    13201 gtcctttcat ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt tcgatgtcgg
    13261 tggcgtagat gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg ctcactttgg
    13321 gcaaaatcgc gcggacgtgt tcgctgtcaa tacacaaaaa ggctttgccg tagaagggca
    13381 tacggtggat gaaatcgata aacgcctgat gcagtttttc gacgctgtgc ccgtaggtat
    13441 ccatatggtc ttcgtcgata ttggtaacga cggacataat cggtgtcagg tgcagaaagg
    13501 atgcatccga ctcgtcggct tcggcaacga tgtattcgcc tttgcccaag cgggcgttgg
    13561 tgcctgcggc gttgagtttg ccgccgataa cgaaagtcgg gtcaagtcct gccgcgccga
    13621 ggatggaggc ggtcaggctg gtggtcgtgg ttttgccgtg cgtgccggca atggcgatgc
    13681 cgtcacggaa gcacatcaac tccgccaaca tcagggcgcg cggaataacg ggaatttgct
    13741 gctccaacgc agcgacaact tcgggatttt cttttttgac ggcggtagag gtaacgacga
    13801 catccgcacc gttaacgtgt tctgcggtat ggccgggata aacttgaatg cccaggctgc
    13861 ccaaatgctc ggtagcggca tttcgcgcct gatccgaacc ggaaacttta aaacccaaat
    13921 tgtgcaagac ttcggcgata ccgctcatgc cgacgccgcc gataccgaca aaatggatgt
    13981 tggtcactcg atttttcatc ataatgttgc gttccggtgg atttcgatgc gtaaaggcgt
    14041 tattttaaag ggctgaccgt ttgcgcgcca tagttttctg acaaatatat agcggattga
    14101 aataaaaaac atccatgccg tctgaacggc ttttcagacg gcatggttcg gcagtttacg
    14161 ccgcacacgc aatcgcggct tcggcaacgt cgtccgcgct gtgcggcaat gccaaagtac
    14221 gggcgttttc tgcccatttg agacattttt cgcggttcag gctgccgagg atttcggcga
    14281 gtttttccgc cgtcaactgg gtttgcggca acagcaatcc cgcctccgcc tgcaccataa
    14341 aacgcgcgtt ggcggtttga tggtcatcaa cggcgtgagg atacggcact aacaacgcac
    14401 ccaatcccgc cgccgtcaac tcggcaatcg tcagcgcgcc ggcacggcaa atcaccaaat
    14461 cggcatcgcg gtaggcggac accatatcgg taataaattc cacgcattcc gcttgcacgc
    14521 ccagcgcgtc gtaatccgcc tgcaagctgc ccagcttgcc ccgtcccgat tggtggtaca
    14581 tctgcggacg cgcattgtcg ggcagcaaag ccaatgcctg cggtacggtt ttgttcaaaa
    14641 cgtccgcgcc caaactgccg ccgaccacca aaattttcag acggccttca cgcccttgga
    14701 agcgttcggc aggcacgggc aggttgctaa tatcggcgcg gacggggttg ccgaccaagc
    14761 cgccttcgtg gctgaacgct ttcggaaaag cgtacaacac ccgcttcgcc cagcgcgaca
    14821 ggtggcggtt ggacaaacct gccacggcgt tttgctcgtg aatcacaatc ggcacgccta
    14881 ataacttcgc cgccaaaccg ccggggaagg taacgaagcc gccgaagccg atgacgcact
    14941 cgacacggtg tttgcggata atccgctgcg cttcgcggac ggtttgatac aaagtaaacg
    15001 gcagcatcag cttgcgtttg atgccgttgc cgcgcacgcc tttaatcgcc agcgtttcca
    15061 agcgtatgcc gtattgcggc acgatacgct cttccatcga atccttgctg cccagccaaa
    15121 tcacatgatg gccgcgcgcg cgcaatgaat ccgccaccgc cagcgcgggg aaaatatgtc
    15181 cgcccgttcc gcccgccatc agcataaagg ttttaccgcc catgatttac tccacccgat
    15241 aaccgcgcat tttccggcgg ttttcataat ctatacgcaa cagcagcatc atgctgatca
    15301 gcatgaaaaa gactgacgaa ccgccatagg acatcaacgg cagcgtcaga cctttggtcg
    15361 gcaaagcacc gatgttcaca ccgatattga agaaactttg gataccgatc caaatgccga
    15421 tacccgaagc gatataggcg ttgaaagtca aacccaaatc gcgcgactgc ttgccgatgg
    15481 aaaacgcccg caccaccagc cagccgtaac agaatatcag cacgcacata ccgaagaaac
    15541 cgaattcttc ggcgatgatg gcaaaaataa aatcggtatg cgcttccggc agaaagccgc
    15601 gtttgctcaa actcgcaccc aaacccatac cgaaccactc tccgcgcccg attgccatca
    15661 gagagtgggt aagctggtag ccggcaccct gcgggtcttt ccacgggtcc aaaaatgcca
    15721 ctacccgctg cacacggtag ggagcggcgg taatcatcag caccatcccg cccaagacgc
    15781 tgcctaccag gacgaaaaaa tatttccacg gcaatcctgc caaaaacagc attccaacgg
    15841 caatgacggt aatgacgaca aacgaaccga aatccggctg taccattatc agcaccaaac
    15901 cgaacgccac cagcataatc ggcaggatga tcgcccggaa acggccgtac atttctaatg
    15961 tttcacgacg tgcctgcgga ttggtggcgg acatgatcag attggccgtc ccccgccaaa
    16021 tcgactgcca acccaaactt tccatgctgc gcaacacttc ttcacggcgc gtgaacaggc
    16081 ttgccaaata aaggatgacc gccagcttga acagctcggt cggctggaaa ttcaacggac
    16141 ccaaaggtat ccaacgggtc gcgccattga tttcgcgccc ggcaatcaat acgactacca
    16201 gcaacaggcc ggatagggca aaaatccacg gcacaagccg ccgccatgtc ctcatcctgc
    16261 aaagaaacca taaaaaaccg ctcgctatca agccggcaac gacgaacccc gcctgtctgg
    16321 tcaaatagaa aaactgatcg ccgccttctt ttgatgccaa atacacagaa gccgaataaa
    16381 tcatcagcag gctgaacgcc gtcatcagca ccaccatcca caaaagcggc gcgtcgaatt
    16441 tcctgccgtc gcgcacaatc ggcctgtcga gcagcagagt gtggacaccg tcgcccactt
    16501 ttaccaatac ttccgaaatc ttcaaaaaaa ccacctgcca gtctgtttgc acctgccgca
    16561 aagggcaaaa atttcagacg gcagacaatg ccgtccgaac atacgataca tcccaaatcg
    16621 gtattctaaa tctttacttg ccgcccaaca atgacggcgt ttgcgtttca gacggtatca
    16681 caaagcctta aacgccccga taaacacttc cgaacggtgc gcatagcctt taaacatatc
    16741 gaagctcgcg caggcggggc tgagcagcac gatatcgccc gcttcggctt gggcatatgc
    16801 cttctgaacc gcttcttcca aagtggcgca gtcggtcata ttcaaaccgc agccgtccaa
    16861 atcgtggcgg atttgcggcg catcgacgcc aatcaggaac acgccttttg ccttgccggc
    16921 aagcgcgtcg cgcaggggcg tgaagtcctg ccctttgccc atgccgccca aaatgacgaa
    16981 tagcggattt tgcaaaccgg caatcgcagc ggcggttgca ccaacgttcg tgcctttgct
    17041 gtcgtcgatg aacaccacgc cgtttttctc gccgattttt tccacgcggt gcggcaggcc
    17101 ttgaaaggtt ttgacgtgtt cgagcaattc atttcgcggc aaaccgacgg cttcgcacaa
    17161 ggcgacggca gccatcacgt tggcggcatt gtgcaggcct tgcaacggaa tgtcttgcgt
    17221 ggcaatcaaa tcttcattgc cttgtttcag gcggcctgtc tcgcgttcca accaaaaatc
    17281 ggcttcgtgt tccaacgaaa accattttac ctcgcgcccg gcgcgcttca tggcacaaca
    17341 gaacgtatcg tccgcattca aaacctgaac gccatcgcca cggaaaatct tgtccttggt
    17401 atgcgcgtag tcgagcaggt cgtcgtagcg gtcgagatgg tcttcggaaa tgttcagcac
    17461 cgtcgccgca gtcgggcgca ggctttcggt gttttccagt tggaagctgg aaagctccaa
    17521 cacccacacg tccgcctttt tgccttcacg ctgcaattcc gcctctaaaa ccggcgtgcc
    17581 gatattgccc gcgataacgg tatccagccc gcacttgatg cagagatagc cgactaggct
    17641 cgttaccgtg gttttgccgt tgctgccggt aatcgcaatt accttgtcgc cccggcggtt
    17701 cacaatgtcc gccagcaatt cgatgtcgcc caacacgcgt ccgccgtttt gcttgaacgc
    17761 ctcaatatcc ggctgccgct cgctgatgcc ggggctgagt gccagaatat cgaaaccgtt
    17821 gtccagcgca tctttcagac ggcccgtgta aaacaccaac ccgtcaaaca tcttaccgat
    17881 ttgcgacacg cgttccggct tcagctccgc atcatacgca gcaacctccg cgccgttttt
    17941 gcgcaggtag gcaatcatgg aaatacccgt accgccgagt ccggcgacga ggattttttt
    18001 gttttgaaaa gtcattttgg tttgtcctaa aacaaatcat attgagcagg agatgtccgc
    18061 ccctgcccaa gccgctttca gacggcatcg cgagctgttc aataacccgc tttcaggcgt
    18121 tggtcattgt cgcagctgtc ttggcatccg ttttgacaag ccttgccaag ccattcttgt
    18181 gcaagggcgc ggtcttggcg cacgccgcgt ctttcggcat acatcacgcc caaattgttt
    18241 tgggctgctg catttccctg tgctgccgcc tgcaagtttt cccgaaaatc cgatacgtca
    18301 tccgcccaca ccgcttggtt caagcccaag gcaatcaggg cggcggcaag ccatttgact
    18361 gtctgtttca tggttttact tctgttttag tataagccgc cgttaacgat agggctgggc
    18421 ggattgtcgc cgcaggttta ttgcgcgttc aaatgccgtc tgaaaggtgt tcagacggca
    18481 taggttcagc ggattttgag ggtactcaaa ccgatcaaca ccaagacgat ggtaataatc
    18541 caaaagcgga cgacgacttg ggtttctttc cagccttttt gttcgtagtg gtgatggatg
    18601 ggcgccatca ggaagatgcg ttttttggtt ttcttatacc agccgacctg aagcataacg
    18661 gatacggctt ctacgacaaa taatccgccc ataatgacga ggacaaactc ttggcggacg
    18721 atgacggcga cggtaccgag cgcggcaccc aatgccaatg caccgacatc gcccataaag
    18781 acttgcgcgg gataggcgtt aaaccacaag aaaccgaggc acgcgccgca catggcggta
    18841 cagaaaatca ccacttcgtt tgcgccggca acgtaaggta attgcaggta ttgggcaaat
    18901 tgtgagtggc cgctggcata ggcgaagatg gcgaggccgg cggcaacgag gacgacgggg
    18961 aaggtcgcaa ggccgtccaa gccgtcggtg aggttgacgg cattggatgt gccgacgatg
    19021 gtcaggtaag acaacaccaa aaagccgacc acgcccagcg gcagggcgat ttgtttgaag
    19081 aacgggacaa tcaaaatatt gttggcggaa ttggcggcaa ggtaaaacaa tgccaaaccg
    19141 gcgataatgg caacgcttga ctgccacacc attttgaatt tggcggacac gccgttgggg
    19201 tctttataga cgactttgcg ccagtcgtcg taaaaaccga gtgcgcccgt ggcgagcaat
    19261 acgcccaaga gaatccagat atacgggttt gcccagttgc cccacaacag ggtggacacg
    19321 gtaatggcgg tcagaatcag cgaaccgccc atcgtcggcg tgccgttttt gacgaggtgg
    19381 gtttgcggac cgtcggtacg cactgcctgc ccgcatttga gcgcggtcag cctgcgtatc
    19441 gtccacgggc cgaacatcag ggaaaacgcc aaggcggtca acgccgccat gacggcgcgg
    19501 aatgtggtgt attgaaaaat attcagaccg gttaaccagt tgctgaaatg tgcgagccat
    19561 aaaaacatgg ggcttccttt ttttgttttt gtcgttacat gggcttgtgc ccgtttgagg
    19621 ttggcttgcc gcccggcaag gtttcggacg acttttgcag attaacgtgc agagactttc
    19681 ccaatcacgc aaatcgggta actccccgga tttttgccgc cacccaagtc taaacataaa
    19741 tcttcataaa gatacgcctg cgtctgcatt ccggcataaa acgcgccgac cagcgcggca
    19801 atcaacaata aggctttaaa aaaacgtcga cttttcattt tcacttatcc tccaatgcct
    19861 cgaccacttc ttccatctgc ataaagcgcg aacctttcac caacacggtg gcgcgttcgg
    19921 gcaaatcgtg gcgcaacact tgaatcaacg ggtctttggc ggcgaaccac aaaccgtccg
    19981 cgccaaattt ttccgccgct tcgacgctgt tgtcgccgac aaaataagcc gcttcgatgc
    20041 cttggtcgcg ggcgtacgcg ccgacttcgg cgtgcatggc ggcggcttcg tcctcaccca
    20101 gttcgcccat atcgcccatc acgaaaatac gcggcgcagg catacgcgcc aacacgtcaa
    20161 ccgcagcttt catgctgtca gggttcgcat tataagtatc gtcaatcagg gttgcgccct
    20221 tgattccgga tttgacgttc agacggcctt tgatattgct gaagcctttc aaaccttccg
    20281 ccacatcgtt caaactcaaa cctgcagcca aagacagcgc ggcggcggcg gcggcgttgt
    20341 ggacattgtg gcggccggga acgggcagaa ccacggctgc gcactcgttg ccgcacacca
    20401 aatcaaattc gcacgacaac ggtttcagca cgatattttc cgcgtggaca tcgccgctat
    20461 cgatgccgaa agtgcgcgta ttcaaattaa gcgttgccgt tttgaagaca gccatattgg
    20521 catcttcttg aggaatcagc gccatgccgt ctgaacataa gccttgataa atctcgcttt
    20581 tcgctttggc aatatcgccc actccgtcga aaccgcagcc gacatgggcg cgcatggcgt
    20641 tgttgaccaa tgcggcatcg ggtttggcga tttgtgtcaa aaccgccagt tcgccaaaat
    20701 ggttcatacc catttcaatc acggcatagc ggtgtttttc gtttaatttc aacaaagtca
    20761 acggcaatcc gatgtggttg ttgaagttgc ctgccgtcgc caaaacggca ttatcgccga
    20821 aacggcggcg caataccgca gccaacattt ccttcaccgt cgtcttgccg cccgagccgg
    20881 taataccgaa cacaaacggg ttcacattct cgcgccacgc cttcgccaac atttgcaacg
    20941 cggtaagcgt gtcatcgact ttcaacgcgc catccattgc aacgcaatct tcgcgcgaaa
    21001 ccacaaccgc cgccgcaccc gcagccaata cgtcttcaac aaaatcatgc gcatcaaacc
    21061 gcccgcccgc caatgcgaaa aacacatcgc ccgcgcggat gtcgcggctg tcggttacga
    21121 tgcgcgacac gggtttgctt tcagacggca tcggaagctt gagggcttgg cagatgaaat
    21181 ttaggtccag tggtttcata tttactttcg ttaatattcg ggcggcggac acatcggtag
    21241 cggctgattt ttttatcgcc tgttttgctg tggtaaaaca cagattattt tcccattctc
    21301 attcggcatt ttttctgtac gtatcatttt ttagacgtat ttttagtcga tttgcctttt
    21361 cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga taaaggcaag gttattgcct
    21421 tcgccctgca catcgggaac attcccccaa aaatcatagc cgtcatcggg caactcgtcg
    21481 gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat taaacgcgct gcgcctcact
    21541 ttaatcattt cgcgccgcgt atcatcggac gaaattttaa ccagcggcac gccgtagctt
    21601 tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc ctttaccgac atgcatcaaa
    21661 ccgtggtcgg aaaagtaaac caaagaccag ctttctttat tttcattcaa tatcttaact
    21721 gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa catagcagga tattttttca
    21781 gtttgatact gaaaccgccg cgcatcctta tccaaacgtg tgcaaaaatc actgtgcgaa
    21841 cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg catttcctat caaaacccgt
    21901 ttgaacgccg gcaacaaaag gcggtcgctc aaccccgcgc ttttgccata atcacccctt
    21961 tgggtaaacc acggataatc gctgcgtagg gcataggtgg aaatttcgtt ggcaaaatgc
    22021 cccaacattc cttgattaga cagccacgcc gtccgaaaac ccgcctgctt cgccaagctg
    22081 acgatgttat tgttcggttc tcccggcagc cccaaagtct gcggcagcga aagattcgtc
    22141 gcgtgggcgg tcgattggta accgtttatc aacagccctt tggtctgact caaaaaaggc
    22201 gtatcgggca atgggaaacc gtaaacattc atataatccg aacgcgcgct ctcaccgata
    22261 accacaacat aatttttata cttgggcgca acatgacgga tatgccatgt tgacgctttt
    22321 tttgctgctt ccaaaatgtg ggcgcgcttg gcggcatatt gtgccggcgc ggaagccaaa
    22381 tcgtaataca ggtcgaaaat attcaacaac aggccggcat caggttctcg caaatcttta
    22441 tcgctggcga ttttatccat caccgcgcag gacaaaatca aagtcaataa tatagtcagc
    22501 catattttgc tgcggcgttt atagtttttt acgtcagcaa ataccccccc cggtacacaa
    22561 tatctccaaa ctgtcaagcc aaaaatgaaa aataatgcct gcacaaaata aagcgacccg
    22621 ggaagattgc cgacaaattc acgcgcctcg gcaggattgc tttccaatat cgaaccgact
    22681 atctgataag acggcgcacc atacagccag ccgaccggca aatatagggc ggttgtgccg
    22741 acgtaaatca gtaaaacaac tgaagaaacg cgcgggaaac cgcgtgccaa taataataaa
    22801 aatacaaccg aagccaaagc acccacggca gaataacggt agccgtattc atattccaag
    22861 tgataacctg atgcgatggc cgcgccaaaa caaaaggcta aaagcgcgca aatgcccaat
    22921 gtatttttta atgtctgatc catatttttt attcgggcgg attgtttcat acgatgccgt
    22981 ccgaatatcc cattatttac gagttaacaa agcctgtccg acgatttcaa gatcggaaaa
    23041 acggtgcttc acgccttgta catcctgata gttttcatgc cctttgccgg caatcaggat
    23101 gatgtcgttt gcggcggctt gttcaaccgc ataacggatg gcggcggcac ggtcggcttc
    23161 gacgcattcg ggcgcgggaa cggcaggcag gatgtcgttg atgatgtcgt gcggattttc
    23221 caaacgcggg ttgtcgctgg tgacgacgac tttatccgcg ccctgtacgg ctgccgcgcc
    23281 catcagcggg cgtttgccgc gatcgcggtt accgccgcaa ccgaatacgc accacaaagc
    23341 cgcaccctgc ggtttgattt cctgcaaggt ggcgagtgct ttttccaatg cgtcgggcgt
    23401 atgggcataa tcgacaacga ccaaaggttt gccgctgttc atgatgcagt ccatgcgccc
    23461 tgaagcggga cggatttttg ccagcacatc caatacttta tcaagcggat agccgttggc
    23521 gcacagcaag gcgatgcagg cggcgaggtt ttgcgcgttg aaccgtccga gcaggcgcgt
    23581 gcggcatttc ccttcgcccc acggggtttg gaatacggct gctatgccgt ctgaagaggc
    23641 ggtgaaatcg ataatgcgga tgtcggcgtg ttcgctgaag ccgtagctat aaacggccaa
    23701 atcgggacag tcttttttca gacgacctac gagttccgcg ccgtattcgt catccacgtt
    23761 gatgactgcg tgtttcaagc cgtgccagta aaacaggcgc gacttgatgg caccgtaggc
    23821 ttccatcgtg ccgtggtagt cgaggtggtc gcgggtgagg ttggtaaaga ttgcgctgcg
    23881 gaatgacacg ccgttgacgc gcgactggtc aagcccgtgg ctggagactt ccatcgcggc
    23941 gactgttgcg ccttgttgac ggaaacggta gagcagggtt tggacatcga cgggggcggg
    24001 tgtggtatgc gtggtttctt ccaatgcacc ccaaaagccg ttgccgaccg tgccgacaat
    24061 ggcggttttt tcgcccaaca aatcggcagc ttgcgccagc cattgcgtga tggaggtttt
    24121 gccgttggtt ccggttacgc cccagaattt gagaccgtct gaaacgttgc cgtaaacttg
    24181 cgccgccaat atgccggcac ggtgtttcaa atctttgatg ccttgattgg ggactttcca
    24241 ttcgggattc cacgcaaatt tgccgtcgtc gtcccaaaaa acaaaagccg cgccgttggc
    24301 aacggcggcg gggatataac tgcgtccgtc ggcatattcg cccggacagg caacgaaaat
    24361 atcgccttgt ttgatttggc ggctgtctga atgcaacaaa cgccctgccg cgtttgcaca
    24421 cgacagggtc gggatgccgg tttcagccaa aggggttaac ttgctgaaca taaaacaatc
    24481 tcgttgatac tcggattaag acggtgtttt gacggctgcg gcggtcaggg gcttggtcgg
    24541 ggaaatgccc aagatgttca ggctgccgcc cataattttt ttgaagggcg gccctgccac
    24601 tacgccgccg taataaccgt gggcagtcgg ttcgtcaatg gttaccgcca caatcacacg
    24661 ggggtttttg gcgggggcaa aaccgataaa ggtagcgatg tgtttgttgt cggcataacg
    24721 cccgttgacg aacttgcgcg ccgtgccggt tttcgcgccg acatcgaaac cgtccaccgc
    24781 acccgccgta ccggtgccgc ccggctcggt tacggaaacc atcagattgc gtacctcgcg
    24841 cgcggtcgat tctttgaata tgcgtttgcc ttgcggcgca accgcctgtt tttcaaagct
    24901 gaccggcagc aaaacgccgt cgtgcgtcag tgcggtatag gcgcgcgcca attgcagcag
    24961 gctcaattgc aggccgtaac cgaaagacat cgtcgcctgt tcaatgggcc gccacctgcg
    25021 ccaatttctc aacaaacctg cagtttcgcc cggaaagccc gagtgcatac gcacaccgat
    25081 gcccaactca tgatagaagt catacatttc ttcggcaccg aaacgcgcag acagtttgct
    25141 tgtgccgacg ttggacgatt tctgcatgat gccgcgcaca tccaaagagg ggtaaacatg
    25201 ggtatcgcgc acgggagacg gtccgatttt ataaggctgc gtattcagcc gttcgttcaa
    25261 atcggttttg cccgcatcca atgccttcgc aatcacaaac ggtttgattg ccgaaccggg
    25321 ttcgatcata tcggttacgg cacggttgcg ccgctgttcg ctgtctgccc ggccgggcct
    25381 gttgggatcg taggcgggcg tattggccaa ggcgaggatt tcccccgtgc gggcatccaa
    25441 aaccaccacc gttccggctt ttgcctgatg gtattcgacc gccttgttca actcttcata
    25501 ggccaaggtc tgaatcctct gatcgaggga aaggatgatg tctttgccgt ttttcggggc
    25561 tttattgcgc ggggagtcca agctgtccac aatattgccc tgccggtccc gcaaaacgac
    25621 ttccgcgccg tcttcgccat gcaggctgtc ttcaagcgaa agttccaaac cttcctgacc
    25681 tttgccgtca atatcggtaa atccgatgac gtgtgcaaac aggttgccca tcgggtaatg
    25741 gcgttttaat tctttttcaa atacaaagtt ttccaaaccc aaggctttga cctcttcggc
    25801 aaccttggga tcgagctgcc gcttaatcca gataaacgac ttgcctttct gttcgagctt
    25861 gttcctcaaa acatcaaccg gcacatcgac aagctcggac aggcgttcca attgtgcggc
    25921 agacggcatt tccttcatct ctttaggcac ggcaaacagg gactccgtcg gcgcactcaa
    25981 cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt gtagccggca atgtttgagt
    26041 ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta tacgttaccg tctgcagata
    26101 cagtccgcga gcaatcagac cggcaaacaa gaccgctatt gccatcagga cgaagctgat
    26161 ccgtccgtta ctggtcatcg gctttttgac ctgctcttct ttgggcagca tccgaggctt
    26221 atattcgctc ttaatcaaca tttttacttc tcgttattat tatcctgacg caggaatccg
    26281 attccggcac acaggctgct tctatctttg atgctccacc ataaaggtat tgcccgaaac
    26341 cggcggatgg aggttttgtt tttctgccgc cgccctgatc gcttcgtggt tcgccaaacg
    26401 cgcctgttgc agcctcattt gcgcataatc ctgctccaag gcgatttcct gttttttcgc
    26461 cttatccaaa gctgtgaagt tgagcctgta ctggttttgc tgcatcacaa cggaaaaagc
    26521 ggaaacgcac accgcaagca gcagaaggaa attcaatttg ttcattgcca ttcagacgtt
    26581 tttctctgtg attgttccgg tatcggaccg gcagtccgct ccgccacacg caaaaccgca
    26641 cttctcgccc tcggattggc ggcaatttcc gcctcacccg gctttaatgc cctgcccacg
    26701 attttcaggg gcagctcggg caaatccgct tccctgaccg ccgcccagcg cggcaggggc
    26761 gcgtgttgcg aatatttttt gacaaactgc ttcacaatgc ggtcttccaa cgaatggaaa
    26821 gcaatgaccg ccaaacgtcc gccctctttc agacgacaca tgacctgcgg caatactgcc
    26881 cctacttctt caagctcgcg gttaataaag atgcggattg cctggaaggt gcgcgtcgca
    26941 ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca cgatctgcgc cagcttgcgg
    27001 gttgtatcga ttggactttc cgcccgttgc gcaacaatgg cgcgcgcaat ctggcggcta
    27061 aaccgctctt caccataatt cttgattacc tcgtgcaaat cctgttccga cgcaaccgct
    27121 atccactctg cggcagacat accgcgcgtc gtatccatac gcatatccaa aggggcatcg
    27181 aaacgaaagc tgaaaccgcg gctgccgtca tcgatttgcg gagacgaaat ccccaaatca
    27241 aacagcgcac cgtccacctt gccgataccc aaaccgtcca atgccgtctg aaacgaagca
    27301 aaaccgccat gcacgacacc gacccgtttg tccgaacgcg ccagctcttc tgccacagca
    27361 atcgcctgag ggtctttgtc gaaaacaatc aaccgccccg catcgcccaa acgcgacaaa
    27421 atcagccggg aatgccctcc cctgccgaac gtaccgtcca catagacacc gtcttcgcgc
    27481 acggcaagcg catccaccgc ctcattcagc aagaccgtga tatgccggta actttctgct
    27541 ccactcacaa ttgcaaatcc gtctgaccca actggaaggc aagttcgtca ggatcgtcat
    27601 ccaaagcctg aaccatctca gcctcccact gctcgcgacc ccaaagctcc aaacggttgg
    27661 cacgaccgac caaaacgact tcacggtcga aatccaccct cttcctcagt ccggcaggaa
    27721 ccagcacccg gccggcgctg tcccattcca aaatttccgc gttatgcagc agaagatttt
    27781 gaaaccgccg taaaacaggg ttatccgcca cttttaagtt taaaagttgc gccgcaacct
    27841 tttcccactc cgcaacaggg tacatcaaca gcttgtgttt cgactcgagc gttaccacta
    27901 cggcaggcgt atagaggcgc gacagaatgt cacggaattt ggcaggaaca gccaaccgcc
    27961 ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat gacatgtccc aagccgaaat
    28021 caaaatcaca agggtaaaag agacactttg ccccacaatt ccccaccaat cgacactata
    28081 agaaatttta aacactcggt caaatcaggg catgaaaact cattaacata tctgaaattt
    28141 ttattccttt taaaaacaat aagataaaaa atgacgacaa cggcacggcg ggtgcggtac
    28201 agaataatcg aaccaataaa caactatata tgattaattt aataatataa acacaatata
    28261 tagtattaag ataaagccat gacagcaccc gtaccaacgt gtaatatgtc gggaaatcca
    28321 ataaatttac acaagctaac acttatcatg cccctccctc tcccctctcc cgaagcacgg
    28381 caatcctcgc tcaaactgca aaccctcatt gccgaaaaaa tcggcaaaca cggcaactgg
    28441 attccatttt cacgttttat ggaattggtt ttatacgctc cgcaatacgg ctactacacc
    28501 ggcggcagcc ataaaatcgg caataccggg gattttatta ccgcaccgac cctcacctct
    28561 ctgtttgcac agacactggc acgccaactt caagaacttc tatcccaaac ggcgggcaat
    28621 atctatgaat tcggcgcggg aaccggacag cttgccgccg atttgttggg cagcatttcg
    28681 gacggcatca gccgttacta tattattgaa atatcgccgg agctggcagc acgtcagaaa
    28741 aacctgattc aagcccgcgc accggaagca tctcaaaaag ttgtccactt gaccgcactt
    28801 cccgaagcgt ttgacggcat catcatcggc aacgaagtac tcgatgccat gcctgtcgaa
    28861 atcgtccgta aaaatgaagg cggctcattc gagcatgtcg gcgtttgcct agataatgac
    28921 cgttttacct attcggcacg accgctgcac gacttacagc tatctgcctt ggcttccctc
    28981 tattttcctc aaacagatta tccctacacc agcgaactac atccgcaaca atatgccttt
    29041 atccgcaccc ttgcctccag actcgaacac ggctgcatga tattcatcga ctacggtttt
    29101 gatgcagcgc agtattacca ccctcaacgc aatcaaggta ctctgatcgg acactaccga
    29161 catcacgtta tccacaatcc ttttgacttc atcggattgg ccgacctgac cgcgcatgtc
    29221 aactttaccg acattgccca agccgggacg gatgccggat tagatttgat aggttacctt
    29281 ccccaatccc atttcttatt gaacctgggc attaccgagc tattggcaca gacggggaaa
    29341 acgaattcgg cagcctatat ccgtgaagct gctgccgttc agaaactgat tgaccagcat
    29401 gaaatgggcg aactgtttaa agtcatcgca ttcggaaaaa atatcggcat cgactgggca
    29461 ggattccgct tcggcgacat ctgccacaaa ctctaaccct catgccgcct gaatccgctt
    29521 cagacggcat aaacttttta acatttaaaa acagtcaact aattcaaaat taaaaaatac
    29581 ggcttgtcaa aaaaacagaa aaacatataa tagcgtcttc acgaaacggc gaattagctc
    29641 agtcggttag agcagaggaa tcataatcct tgtgtccggg gttcgagtcc ctgattcgcc
    29701 accaaatttt cgggggtata gctcagttgg tagagcgctt gcatggcatg caagaggtca
    29761 gcggttcgat cccgcttacc tccaccagat aaaaaagcac agaccgtaaa aaggtatgtg
    29821 cttttttatt acctgattac cggcaaataa agaataaacc actgccttca aaacaggcaa
    29881 tcgactttaa acctatcgcc ccgcctgtcc tgattttata gtgaattaaa tttaaaccgg
    29941 tacagcgttg gctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
    30001 tgatttaaat ttaattcact atatcagccc gccagacaaa cccgacccga ataatgtctt
    30061 caggtcgggt ttatggtttc attcccaact tatccagccc gacaaccaca atattatgat
    30121 gacgatgccg aaaacaatgc ggtaataggc aaacgggata tagtttttct tggaaacaaa
    30181 cttcagcagt gctttgaccg ctactaaacc ggaaacaaaa gcggcaataa agcctatcag
    30241 aatcaaaccg acatcatgca gggtgaaaaa ccggtaatgt ttcaggacat cataagccgt
    30301 tgcggcaacc atcatcggaa cggccaagaa aaatgaaaac tccgttgccg ttttccgctc
    30361 gattccccaa agcatcccgc ccataatcgt actgcccgaa cgggacgtac ccggaaccag
    30421 tgcaaacact tgggcaacgc cgatcatcaa ggcatcaatc ggacgcaatg catcaacatc
    30481 gacaatttta ggctctgccc ggctttggcg tttctccacc cacaaaataa aaaaaccgcc
    30541 caaaaccagc atgactgcaa cactcaaggg gttaaacaga tactctttga tttgtttgcc
    30601 gaacaacagc cccatcacgg cggcaggtat aaaagcaatg gcaagattaa ggacgaaacg
    30661 gttggctttc cggtcttttc ccacgccatg caacacattg ctgaaacgct gccggtattc
    30721 aaacactacc gccaaaaccg caccgagctg gatggtaatt tcaaaaacct tgtgattgct
    30781 gtgaaaatca atcagattgc cgaacacaat caaatgtccg gtgctggaaa tcggtaaaaa
    30841 ttcggtaaaa ccttctacca agcccatcat cagggctttc aggacaatca gaaaatccat
    30901 tgcttgcgct tctttcggat acgggagttc ggctatttct gtacagcagg ggtctgacgc
    30961 ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg tatgaacacc gccgtcaaag
    31021 ccattattga agataacgcg gtattttccg ccgacgacaa cggtcggcgt actgtcgatg
    31081 cggtactgtt ccgtcaattg ctgcattttt gatgcagcag ccgctgccgc aggagagtcg
    31141 taggcgcgca tcagtttttt gccgtcaaag cctttttgag acaaagccca tttttcggca
    31201 accgacctgt tttccaagcg gattttttgt tcgtaaactg ctttaaacac ggcagggttt
    31261 gcctgatatt tcaaacctga cagcttgacc gcagcagcca ttcttgccag accgagcatt
    31321 tcaggctgcc agaccacgtg ctccgtcctt aaataggcat cagacggcaa tgccttgccc
    31381 agtttcaata acaaaggatc gaaatgatgg caatgtacgc agaaatagcc gaaaaattcc
    31441 aaaacctcaa ttttacccga ctgttcttga ggaatgggtt tatccaacac aagatagtct
    31501 tccccttccg tcagggcata tgcctgcgcg gacaacactg ccgacagcag cagcggcaac
    31561 agatgtttga gcttcataat tatttacttt cgatagaacg gatcaggctg gcgacttcat
    31621 gttttttcaa ctcgtcctgc atttttttca ccgcatcggc agacatattg ccgctttgca
    31681 cccggtaaag cgttttatgt cccgcctgat agccgaccac cttggaagat atgcccaaga
    31741 ttgccagttt ggcacgctgc ccttccgcgc tccggcggtc ggcatacgcg cccatttgca
    31801 gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt ctgcacttct ttggcagcgg
    31861 cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg ttccggggtc ggtttgggtg
    31921 caactttttc cttctccgcc tttttctctt cttttgaagc ttttttctct gtttctttag
    31981 atggttttac cgcttgtttt ttaaccgttt cggcatcttt cttctgcgct ttttccctga
    32041 cggtttgttc acgctcttcc gtcagcactt tcttacgcac tgcctgtccg tccggctctt
    32101 cccgttccgg ctcgcccgcc ttttcttcaa cctcgtcggc tttatcggca gctttttccg
    32161 catccgactg ctctgcctct gtcgcagcat ccggttcgta caaggcgttt tgatcggccg
    32221 gttcaggttg gatgtcttcc ttaggctggt ttttcggttt caggatttcc gtttctgcag
    32281 gctgcttcga cgaagccggg attttgaacg cattttgacc gctttggttc agataaaaca
    32341 aaataccggc aataatgacc gtcgccagta tcaaaccgaa gaaaaaaccg gacagacctt
    32401 ttccggattg ggaaaatttg ttcataaaca taccttaatg tgtttcagac ggcattaacg
    32461 ccgtttgctt acgggcggat attctaacaa tatcgccata tttgggcaaa acctgcttcc
    32521 attcccgttc ctataaagcg cggcggaaac ctatggcttt catgacgtgg cttctgccga
    32581 cttcttcgtc gcccgccaaa tccgccaatg tacgcgccac gcgcataatg cggtggaagc
    32641 tgcgggcgga aagggagagt ttttccagca ggccgcccaa tgcttcctgc gcttcttttt
    32701 ggatgcaggc ttgggaatcg agttcgctga cgctcaaagc ggcgttgact ttgccctgcc
    32761 gtgcgtattg tttgtcgcgg gcggcgataa cgcgttccaa aacggacgcg ctgctttcgc
    32821 ccgcttcctg ctgcatcagt tcggcggcgg acaggctggg gacttcgatg gtcaaatcga
    32881 tgcggtcgag cagcggcccg gaaatcttgc tgcggtaacg cgcgacgctt tcgggcgtgc
    32941 agcggcaggg tttgacggga tgcccgagat aaccgcacgg gcaggggttc atggcggcaa
    33001 caagttggaa tttggcagga tagacggctt ggcgcgccgc gcgggaaatg tggatttcgc
    33061 cgttttccaa cggttcgcgc aaaacttcca aaactttgcg gtcgaactcg ggcagctcgt
    33121 ccaaaaacaa tacgccgtgg tgcgccaatg aaatctcgcc cggacgcggg tcggaaccgc
    33181 cgccgaccat agccgccgcg ctggcgctgt gatgcggact gcggaaagga cggttgctgt
    33241 cgagttgttg ctggtggttg ggcaggagcg aacgcaatgc ccaaacttct accaattcgt
    33301 cttcggtcag cggcggcagg atgccgggca gccgttggga gagcatagac ttgcccgttc
    33361 ccggcggacc catcatcaag aggctgtgtc cgcctgcggc agcgatttcc aaagcaaggc
    33421 gcgcggtgtg ctgacctttc acatcgcaca aatcaggttg tccgccatgt tcaaacggca
    33481 tctgaggaac ttggcattcg gtttgcgcca aaggttcgat gccgttcaaa tgggcggcga
    33541 cttcgcccaa agagcgcgcg ccgtaaacgg taatgccgcg catcacggcg gcttgtcctg
    33601 cattttcttc aggcaaaaca aatgcacgtt ttgcctgcat accctgccac gccatcgcca
    33661 acgcgccgcg cacggggcgc aacagccccg acagtgccaa ttcccccgca aactcgtatt
    33721 ccgccagttt ttcgggcgca acctgccccg atgcggcaag gatgccgatt gcaatcggca
    33781 aatcgaaacg ccccgactct ttgggcaggt cggcgggggc gaggttgacg gtgatttttt
    33841 tggcggggaa ttcaaaaccg ctttgaataa tggcggcacg gacgcggtcg cgactttctt
    33901 tgacttccgt atcgggcagc ccgacgatgt tgaaatgcgg cagaccgttg gcaaggtggg
    33961 cttccacttc gaccaacggc gcattcatac cgctcaaggc gcggctgtaa accaaggcaa
    34021 gcgacatatt tcagacggcc ttattcgccg gcttcggttt gctgcctgat ttcggcgacg
    34081 gcttcttcgg cagcggcttc agccgcttcc aatgctgccc gttcgggatt ttgcgcggct
    34141 tcgagttttg ccaaacgcgc ttccaaagcc gccagtttgg tacgggtttt gattaaaacc
    34201 tgctgctgga tgtcgaattc ttcgcgcgta accagatcca tacggttgaa cgcgccgccc
    34261 agcatcgcct taatattttt ttccacatct ttggcagggc tgttggcgat ggtttcgctg
    34321 attttcgagc cgacttcctc aaaaagctgc ttgccgaaca taatctgtat ccttcctgaa
    34381 catatcaaat tcaatcggct attgtataag gaaaaatgcc gtctgaaaac gggcggcgga
    34441 taatcggcaa aacataccgc gcctcctttg cggttgctaa attacacctc aaaacacctg
    34501 ccgcctgaaa acggatttca tattccgcgc aacgcctgat tacaagacac tacaaaacaa
    34561 tatgctgttt taaatgattt ttccgacgcg catcgtttca aactcggctt tttaagccat
    34621 taagtgcttt gcaaacaaca gaaattgggt tatgctgaaa cggattattt acaatttcat
    34681 atagttttat tacatatctt attgtgattg aagataattt atccgaatcc ccctttcggg
    34741 tatccggatt ttccgttgta cttttattag aaaaacattt caggcgcaag ttgcttgcaa
    34801 tttcaaagcc tgtaattctg aagttttaga cgaggaatag cacatgaatc ccatgtacat
    34861 tacttttgca atctatctgg ttgcagtcct tctcatcggg cttgccgcct atttttctac
    34921 gcgcaatttc gacgattata ttttgggcgg gcgcagccta ggcccgtttg ttaccgcgat
    34981 gtcggcaggc gcgtccgata tgtccggctg gctgctgatg ggtctgccgg gcgcgattta
    35041 cctgagcggt ttgaatgagg cttggattgc catcggcctc ttggtcggcg cgtatttcaa
    35101 ctggctcttg gtggcgggcc gcctgcgcgt gcataccgaa tacgccaaca atgcgctgac
    35161 gctgccggat tatttcttcc accgcttcgg cgcgggcgga cacttgatga aagtggtttc
    35221 cgcactgatt atcctgttct tcttcacgat ttattgcgcc tcgggcattg tggcgggcgc
    35281 aaccctgttc caaagcctgt ttgaaggtat gacttacaat caggcaatgt ggctgggcgc
    35341 gggcgcgacc atcgcctata ccttcttggg cggcttcttg gcggtcagct ggacggatac
    35401 gctgcaggct tctttaatga ttttcgccct gattttaacg ccggtgatgg tctatctggg
    35461 cttgggcggc gcggaacaga tgtctgccgc gattcaaagc gtcgccgcag gcacgggcaa
    35521 agcatacggc agcctgtttg ccggtacgac cgtcatcggc atcatttcca ccgccgcatg
    35581 gggcttgggc tatttcggac agccgcacat tttggcgcgc tttatggcgg ccgaaagcgc
    35641 gaaatcgctg gtatccgcac gccgcatcgg catgacttgg atggcgttgt gccttgcggg
    35701 cgcggtggcg gtcggttatt tcggcattgc gtatttcggt gcaaatcccg acaaagtttc
    35761 ttctatgagc ggcaaccacg aacgcatctt catcgcgctt tccaccctgc tgttcaaccc
    35821 ttggatcgcc ggcatcattt tgagcgcgat tttggcggcg gtgatgtcca ccctgtcctg
    35881 ccagcttttg gtttgctcca gcgcgattac cgaagacttc tacaaaggct ttttgcgtaa
    35941 aaacgcgcaa cagtcggaac tggtatgggt cggccgcctg atggtcttgg cgattgccgt
    36001 gatttccatc ctgattgctt ccgatccgaa cagcaaagta ttgggactgg tgtcttatgc
    36061 gtgggcaggt ttcggtgcgg cgttcggccc gattgtgatt ctgtccgtat tgtggaaacg
    36121 catcactgcc tacggcgcgt tgtcgggtat ggttgcaggt gccttaaccg tagtcgtatg
    36181 ggcggaatgg ataaaaaaac cggctcaagc agcaggcgaa agcggcttgt tgacgatgta
    36241 cgaaatcgtc ccgggcttta ttgtttgctt aatcattgcc gtgttggttt ccctgttcaa
    36301 caaagaacct tcgcgcgaaa tccaagaacg ctttgaaaaa gccgacgcag attaccgcgc
    36361 cgcccgataa acccctttca gacggcacgg catcccggtt cgtgccgtct gaacccgttt
    36421 ccgggtcaaa cacaaataaa ccttgcctgc atacaggcct tatttgtgtt ttacccgttt
    36481 cattttcaaa gaaacggaag cacctaccct gcccgcattt gaagccttgt atttcatctg
    36541 cggatttatt acgatattac cgtattcagg ccgcaccgat gccgcctgcc ccccgaaaaa
    36601 ctttggagaa tccaaaaaat gtttcatttt gcatttccgg cacaaactgc cctgcgccaa
    36661 gcgataaccg atgcctaccg ccgtaatgaa atcgaagccg tacaggatat gttgcaacgt
    36721 gcacagatga gcgacgaaga gcgcaacgcc gcctccgagc ttgcccgccg tttggttacc
    36781 caagtccgcg ccggccgcac caaagccggc ggcgtggatg cgctgatgca cgagttttca
    36841 ctctccagcg aagaaggcat cgcgctgatg tgtctggcag aagccctgct gcgtatcccc
    36901 gacaacgcca cgcgcgaccg cctgattgcc gacaagattt cagacggcaa ctggaaaagc
    36961 catttgaaca acagcccttc cctcttcgtc aatgctgccg cctggggcct gctgattacc
    37021 ggcaaactga ccgccacaaa cgacaaacaa atgagttccg cactcagccg cctgatcagc
    37081 aaaggcggcg caccgctcat ccgccaaggc gtaaattacg ccatgcggct tctgggcaaa
    37141 cagttcgtaa ccggacagac cattgaagaa gccctgcaaa acggcaaaga acgcgaaaaa
    37201 atgggctacc gcttctcctt cgatatgttg ggcgaagccg cctacaccca agccgatgcc
    37261 gaccgctact accgcgacta tgtcgaagcc atccacgcca tcggcaaaga tgcggcagga
    37321 caaggcgttt acgaaggcaa cggcatttcc gtcaaactct ccgccataca tccgcgctac
    37381 tcgcgcgccc aacacggccg tgtgatgggc gaactgttgc cgcgcctgaa agagctgttc
    37441 cttttgggta aaaaatacga tatcggtatc aacatcgatg ccgaagaagc caaccgtctg
    37501 gagctgtctt tggatttgat ggaggcactc gtttccgatc ccgatttggc aggctacaaa
    37561 ggcatcggtt tcgttgttca agcctaccaa aaacgttgtc cgttcgttat cgactacctg
    37621 atcgaccttg cccgccgcaa caaccaaaaa ctaatgatcc gcctcgtcaa aggcgcgtat
    37681 tgggacagcg aaatcaaatg ggcgcaagtg gacggcttga acggctatcc gacctacacc
    37741 cgcaaagtcc acaccgacat ctcctacctc gcctgcgcgc gcaaactgct ttccgcgcaa
    37801 gacgcggtat ttccgcaatt cgccacccac aacgcctaca ccttgggcgc aatctaccaa
    37861 atgggcaaag gcaaagactt tgaacaccaa tgtctgcacg gcatgggcga aaccctgtac
    37921 gaccaagtcg tcggcccgca aaacttaggc cgccgcgtgc gcgtgtacgc cccggtcggc
    37981 acacacgaaa ccctgctcgc ctacttggtg cgccgcctgt tggaaaacgg cgcgaactcg
    38041 tctttcgtca accaaatcgt cgatgaaaac atcagcatcg acacgctcat ccgcagcccg
    38101 ttcgacacca tcgccgaaca aggcatccac ctgcacaacg ccctgccgct gccgcgcgat
    38161 ttgtacggca aatgccgtct gaactcgcaa ggcgtggact tgagcaacga aaacgtattg
    38221 cagcagcttc aagaacagat gaacaaagcc gccgcgcaag acttccacgc cgcatccatc
    38281 gtcaacggca aagcccgcga tgtcggcgaa gcgcaaccga ttaaaaaccc tgccgaccac
    38341 gacgacatcg tcggcacagt cagctttgcc gatgccgcgc ttgcccaaga agcggttggc
    38401 gcagccgttg ccgcgttccc cgaatggagt gcgacacctg ccgccgaacg cgccgcctgc
    38461 ctgcgccgtt tcgccgacct gctcgaacag cacacccccg cgctgatgat gcttgccgtg
    38521 cgcgaagcag gcaaaacgct gaacaacgcc attgccgaag tgcgcgaagc cgtcgatttc
    38581 tgccgctact acgcaaacga agccgaacat accctgcctc aagacgcaaa agccgtcggc
    38641 gcgattgtcg ccatcagccc gtggaacttc ccgctcgcca tctttaccgg cgaagtcgtt
    38701 tccgcattgg cggcaggcaa caccgtcatc gccaaacccg ccgaacaaac cagcctgatt
    38761 gccggttatg ccgtttccct catgcacgaa gccggcatcc cgacttccgc cctgcaactc
    38821 gtcctcggcg caggcgacgt gggtgcggca ttgaccaacg atgcccgcat cggcggcgtg
    38881 attttcaccg gctcgaccga agtggcgcgc ctgatcaaca aagcccttgc caaacgcggc
    38941 gacaatcccg tcctgattgc cgaaaccggc ggacaaaacg ccatgattgt cgattccacc
    39001 gcacttgccg aacaagtctg cgccgacgta ttgaactccg ccttcgacag cgcgggacaa
    39061 cgctgctccg ccctgcgtat cttgtgcgtc caagaagacg ttgccgaccg tatgctcgac
    39121 atgatcaaag gcgcgatgga cgaactcgtc gtcggcaaac cgattcagct cactaccgat
    39181 gtcggccccg tcatcgatgc cgaagcacag caaaacctgt tgaaccacat caacaaaatg
    39241 aaaggcgttg ccaagtctta ccacgaagtc aaaaccgctg ccgatgtcga ttccgaaaaa
    39301 tccacgttcg tccgccccat cctgtttgaa ttgaacaacc tcaacgaact gcaacgcgaa
    39361 gtcttcggtc ccgtcctgca cgtcgtccgc taccgcgccg acaaactcga cagcgtcatc
    39421 gaccaaatca acagcaaagg ctacgccctg acccacggcg tacacagccg catcgaaggc
    39481 acagtacgcc acatccgcag ccgcatcgaa gccggcaacg tttacgtcaa ccgcaacatc
    39541 gtcggcgcag tcgtcggcgt acagcccttc ggcggacacg gtctgtccgg cacaggcccc
    39601 aaagcaggcg gttcgttcta cctgcaaaaa ctgacccgca tccccgaatg ggttgccccg
    39661 accctgagcc aaatcggaca ggcagacgaa gccgcgctca aacgcctcga agcactggtt
    39721 cacaaactgc cgttcaacgc cgaagagaaa aaagccgcag cggccgcttt gggacacgcc
    39781 cgcatccgca ccctgcgccg tgccgaaacc gtccttaccg gcccgaccgg cgagcgcaac
    39841 agcatctcat ggcacgcgcc aaaacgcgtc tgggtacacg gcggcagcct cctgcaagcc
    39901 ttctgcgccc taaccgaact tgccgcctcc ggcgtacagg cagtggtcga acccgacagc
    39961 ccgctggctt cctacaccgc cgacttggaa ggtctgctgc tggtcaacgg caaacccgaa
    40021 accgccgaca tcagccacgt tgccgccctg tcgcctttgg acagcgcgcg caaacaggaa
    40081 cttgccgccc acgacggcgc actcatccgc atcctccctt cggaaaacgg actcgacatc
    40141 ctgcaagtgt ttgaagaaat ctcttgcagc gtcaacacca cagccgccgg cggcaacgcc
    40201 agcctgatgg cggttgccga ctgattttac cgaaatcccc gagcgcggcc cgtgaaccaa
    40261 tgccgtctga aaacctttca gacggc
//
LOCUS       DAWKOD010000003        37757 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000003 DAWKOD010000000
VERSION     DAWKOD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.003"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..232)
                     /locus_tag="WJK73_000091"
     tRNA            complement(142..232)
                     /locus_tag="WJK73_000091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="WJK73_000092"
     tRNA            complement(268..354)
                     /locus_tag="WJK73_000092"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            complement(390..476)
                     /locus_tag="WJK73_000093"
     tRNA            complement(390..476)
                     /locus_tag="WJK73_000093"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:cag)
     gene            565..2940
                     /gene="rnr"
                     /locus_tag="WJK73_000094"
     CDS             565..2940
                     /gene="rnr"
                     /locus_tag="WJK73_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ7556638.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKSDLVKCRIEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFGEACAKAAKKIPNHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPHVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIEHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WJK73_000095"
     CDS             complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WJK73_000095"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ7556639.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4883..7441)
                     /gene="glnD"
                     /locus_tag="WJK73_000096"
     CDS             complement(4883..7441)
                     /gene="glnD"
                     /locus_tag="WJK73_000096"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ7556640.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSNSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(7490..7810)
                     /locus_tag="WJK73_000097"
     CDS             complement(7490..7810)
                     /locus_tag="WJK73_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7556641.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8135..8608)
                     /gene="bfr"
                     /locus_tag="WJK73_000098"
     CDS             complement(8135..8608)
                     /gene="bfr"
                     /locus_tag="WJK73_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7556642.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8636..9100)
                     /gene="bfr"
                     /locus_tag="WJK73_000099"
     CDS             complement(8636..9100)
                     /gene="bfr"
                     /locus_tag="WJK73_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7556643.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9563..10546)
                     /gene="lipA"
                     /locus_tag="WJK73_000100"
     CDS             complement(9563..10546)
                     /gene="lipA"
                     /locus_tag="WJK73_000100"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ7556644.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10539..11162)
                     /gene="lipB"
                     /locus_tag="WJK73_000101"
     CDS             complement(10539..11162)
                     /gene="lipB"
                     /locus_tag="WJK73_000101"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ7556645.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11164..11439)
                     /locus_tag="WJK73_000102"
     CDS             complement(11164..11439)
                     /locus_tag="WJK73_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ7556646.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11624..12694
                     /locus_tag="WJK73_000103"
     CDS             11624..12694
                     /locus_tag="WJK73_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ7556647.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLFALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12801..13748)
                     /locus_tag="WJK73_000104"
     CDS             complement(12801..13748)
                     /locus_tag="WJK73_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ7556648.1"
                     /translation="MEFFIILLAAVVVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13763..14170)
                     /locus_tag="WJK73_000105"
     CDS             complement(13763..14170)
                     /locus_tag="WJK73_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ7556649.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14251..14910
                     /gene="ung"
                     /locus_tag="WJK73_000106"
     CDS             14251..14910
                     /gene="ung"
                     /locus_tag="WJK73_000106"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ7556650.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            15012..15659
                     /locus_tag="WJK73_000107"
     CDS             15012..15659
                     /locus_tag="WJK73_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7556651.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            15732..16205
                     /locus_tag="WJK73_000108"
     CDS             15732..16205
                     /locus_tag="WJK73_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ7556652.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            16553..17497
                     /locus_tag="WJK73_000109"
     CDS             16553..17497
                     /locus_tag="WJK73_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7556653.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            17639..17773
                     /locus_tag="WJK73_000110"
     CDS             17639..17773
                     /locus_tag="WJK73_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ7556654.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(18063..18578)
                     /locus_tag="WJK73_000111"
     CDS             complement(18063..18578)
                     /locus_tag="WJK73_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556655.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19044..20966)
                     /locus_tag="WJK73_000112"
     CDS             complement(19044..20966)
                     /locus_tag="WJK73_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7556656.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLTKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(21043..21438)
                     /locus_tag="WJK73_000113"
     CDS             complement(21043..21438)
                     /locus_tag="WJK73_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ7556657.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            21611..22918
                     /locus_tag="WJK73_000114"
     CDS             21611..22918
                     /locus_tag="WJK73_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ7556658.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEEFGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            22911..23345
                     /locus_tag="WJK73_000115"
     CDS             22911..23345
                     /locus_tag="WJK73_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556659.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(23404..23673)
                     /locus_tag="WJK73_000116"
     CDS             complement(23404..23673)
                     /locus_tag="WJK73_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7556660.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(23855..26317)
                     /gene="lon"
                     /locus_tag="WJK73_000117"
     CDS             complement(23855..26317)
                     /gene="lon"
                     /locus_tag="WJK73_000117"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ7556661.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            26568..27671
                     /locus_tag="WJK73_000118"
     CDS             26568..27671
                     /locus_tag="WJK73_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7556662.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVLKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFVDLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(27699..29444)
                     /gene="recD"
                     /locus_tag="WJK73_000119"
     CDS             complement(27699..29444)
                     /gene="recD"
                     /locus_tag="WJK73_000119"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ7556663.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDATGARQNLAKWFQGAGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAHNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLTAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(29512..30207)
                     /gene="lolD"
                     /locus_tag="WJK73_000120"
     CDS             complement(29512..30207)
                     /gene="lolD"
                     /locus_tag="WJK73_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ7556664.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMSMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(30200..31447)
                     /locus_tag="WJK73_000121"
     CDS             complement(30200..31447)
                     /locus_tag="WJK73_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ7556665.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            31653..31931
                     /locus_tag="WJK73_000122"
     CDS             31653..31931
                     /locus_tag="WJK73_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556666.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(31987..32604)
                     /gene="recR"
                     /locus_tag="WJK73_000123"
     CDS             complement(31987..32604)
                     /gene="recR"
                     /locus_tag="WJK73_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ7556667.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(32671..34209)
                     /locus_tag="WJK73_000124"
     CDS             complement(32671..34209)
                     /locus_tag="WJK73_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ7556668.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(34263..34637)
                     /locus_tag="WJK73_000125"
     CDS             complement(34263..34637)
                     /locus_tag="WJK73_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ7556669.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            34787..36415
                     /locus_tag="WJK73_000126"
     CDS             34787..36415
                     /locus_tag="WJK73_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ7556670.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            36487..37740
                     /locus_tag="WJK73_000127"
     CDS             36487..37740
                     /locus_tag="WJK73_000127"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ7556671.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         8493 a         9752 c        10227 g         9285 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggcaatcta
      361 aatattgaaa taaagcaaag catttgattt ggtgcccggg agaagactcg aacttccaca
      421 cccgtgagga tactagcacc tgaagctagc gcgtctacca attccgccac ccgggctttg
      481 ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag aagtcgctat tatatagttc
      541 ataaagagaa tgtcaacagt ccaaatgaat aaaaatatta aatctttaaa tttacgggaa
      601 aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa
      661 tggataatcg aattgttgga gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc
      721 gagctgtcga ttacggaaga cgagtatgtc ttttttgaac gccgtctgaa ggcgatggcg
      781 cgggacggtc aggttttaat caaccgtcgg ggcgcggttt gcgcggcgga caaatcggat
      841 ttggtcaaat gccgcatcga ggcgcataag gacggtttcg gttttgccgt gccgctcacg
      901 cccgccaaag acggtgattt tgttttgtat gaacgccaga tgcgcggcat tatgcacggc
      961 gatattgtca ctgttcgtcc tgccggcatg gaccgtaggg gccgccgcga agggacggtt
     1021 ctggatattg tcgaacgcgc gcaaagcaaa gttgtcggcc gtttctatat ggataggggc
     1081 gtggcgattt tggagccgga agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac
     1141 ggcgtggcgc gtttcaaacc tgaatccggt caggtcatcg tcggcgaaat tgaggtttat
     1201 cctgagcaaa accggccggc agtggcaaaa atcatcgaag ttttgggcga ttatgccgac
     1261 agcggcatgg agattgaaat tgccgtgcga aagcatcatt tgccgcacca attcggtgaa
     1321 gcgtgtgcca aagccgcgaa aaaaattccc aaccatgtac gcaaaagcga tttgaaaggc
     1381 cgcgtcgatt tgcgcgacct gcctttggta acgatagacg gcgaaacggc tcgagatttt
     1441 gacgatgcgg tgtttgccga gaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc
     1501 gatgtcagcc attatgtccg ccccgatgac gctatcgaca cggacgctca ggaacgcagc
     1561 accagtgttt acttcccgcg ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc
     1621 atctgctcgc tcaatcctca tgtcgagcgt ttgtgtgtgg tgtgcgatat ggttatcact
     1681 tacgcgggca atatcaaaga ataccgcttc taccccgccg tgatgcgctc tcatgcccgc
     1741 ctgacctaca accaagtttg gaaatggctt tcaggcggca tcgagcatcc gttcaaaacc
     1801 caaatcgaca cgctttacaa actcttcaaa atccttcaga aaaagcgttt cgaacgcggg
     1861 gcggtggagt ttgacagcat cgaaacccaa atgcttttcg acgacaacgg taaaattgaa
     1921 aaaatcgtcc ccgttgtccg caacgatgcc cacaagctga ttgaagaatg tatgttggcg
     1981 gcaaacgttt gcgcagcgga ttttctgttg aaaaacaagc ataccgcatt gttccgcaac
     2041 catttggggc ccacgcccga aaaactcgcc gccttgcgcg agcagctcgg tctgttgggg
     2101 cttcaacttg gcggcggcga caacccgtcg ccgaaagact atgccgcgct tgccggacag
     2161 ttcaaaggca ggccggatgc cgaattgctg caagtcatga tgttgcgctc catgcaacag
     2221 gcggtttacg aaccgcattg cgacggacac tttggtcttg cctacgaagc atacgcccac
     2281 ttcacctcgc ccatccgccg ctatcccgac ctgaccgtac accgcgccat caaagccgtg
     2341 ttgaatcagc aaacctacac gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg
     2401 ttctgtgagc gccgtgccga cgacgccagc cgcgacgtgg aaaactggct gaaaacctac
     2461 tatatgcgtg acaaagtcgg cgaaatattt gaaggcaaaa tttccggggt ggcaaatttt
     2521 ggaatatttg tcactttgga cgatatccat atcgacggtc tggtgcatat cagcgatttg
     2581 ggcgaagact atttcaactt ccgccccgaa atcatggcaa tcgaaggtga acgcagtggc
     2641 atccgtttca atatgggcga cagggttgcc attaaagtgg cccgtgccga tttggatgat
     2701 ggaaaaatcg attttgtcct gattgccgag gggagcggca gagggcggaa agttaaatca
     2761 tccgcgtctg ccaaaccggc agggacggcg gggaaaggga agccgaaaac cgccgccggg
     2821 aaaaaaacag cccgaggcgg caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg
     2881 gggaaaaagg caaagaaacc ggttccgatt aaggtaaaaa aacggaaagg caaatcataa
     2941 tgctgacggg gcggcttggg gagggagggg catactttcc cgaagccgcc gcccgatttt
     3001 ttgatatggc ggccggcgca tcatttcagg ccaatcgaaa cgcccgtatt gaaagattgc
     3061 gtttatttcc accgccgttt taaaggccag cggtattcgg cagacggggc gcaaacggcg
     3121 ttcagacggc attttcattc tttcggcgtg tccgtccgaa tggctttgcc cgtccgcgca
     3181 atcagcccgt gcggcttgcc ttgaacggac aaaaaatgcc gtctgaaacc cgaaaatcag
     3241 gtttcagacg gcattttgcc ttgaaaaggc tgttcaaatc agcggtggta gttcggtgct
     3301 tctttagtga tttgaacgtc gtgaacgtgc gattcgctca tacctgcgga agtgatttcc
     3361 acaaattcgg ctttttcgtg catttcggca atattggcgc aacccaagta acccatactg
     3421 gaacgcagac cgccggtcag ctggtggatg atgttcacaa tcgggccttt gtaaggaacg
     3481 cggccttcga tgccttcggg gacgtatttg tctgcgctgt cggttttgtc ttggaagtag
     3541 cggtcggcag aaccttggct catcgcgccc aaggaaccca taccgcgata ggatttgtac
     3601 gagcggcctt ggtagagttc gatttcgccc ggcgcttctt ccgtgcctgc aaacataccg
     3661 ccgagcatga cgctgtacgc gcctgcggcg agggctttgg cgatgtcgcc ggagaagcgg
     3721 atgccgccgt cggcaatcag cggaacgccc gtgcctttga gggcttcggc aacgttgtga
     3781 atggcggtca gttgcggcac gccgacacct gccacgatac gggtggtgca aatcgatccc
     3841 ggaccgatac cgactttgac ggcatccgcg ccggcggcga ccaaatccaa tgcggctttg
     3901 gcagtggcga tgttgccgcc gatgacttgg atgtgtggat aggtttcttt cacccatttc
     3961 acgcggtcaa tcacgccttg gctgtgcccg tgggcagtat cgacgacaat cacgtccacg
     4021 ccggcttcga ccaaggcttt gacgcgctct tcggtatcgc cgccggtgcc gactgccgca
     4081 ccgacgcgca gacggccctc ggagtctttg ttggcgttgg gaaactcggt ggtttttaaa
     4141 atatctttaa cggtaatcag acctttgagt tcgtcttttt cgttcagaac caaaacgcgc
     4201 tcgactttgt gcgtgtgcat cagttcgcgc gcttcgtcta tgcttgtgcc ttcggggacg
     4261 gtaaccagac gttcgcgcgg ggtcataatg gcggaaacgg gcaaatcgac gcggttttca
     4321 aaacgcaggt cgcggttggt tacgatgccg acgactttgc cgttttcaac gacgggcagg
     4381 ccggacattt tgcgtttgcg ctgcgcgcgc atttccaaga cttcgcggat gagcgtcgtc
     4441 ggtgcaacgg ttacggggtc tttgaccacg ccgctttcgt ggcgtttcac tttggaaacg
     4501 gcgcgcgcct gcatctccgg cggcatattt ttatggatga tgccgatgcc gccttcctgt
     4561 gccatcgaaa tggcgaggcg cgcctcggta acagtatcca tcgcggcgga aagcaggggg
     4621 aggttgagcg tgatttcgcg ggtgagcttg gtttgaagtt taacgtctcg cggcagcacg
     4681 gtcgaatgtg cgggaaccaa caaaacatcg tcgaaagtat aggctttttc tacgatacgc
     4741 ataatgctcg gtctttcagt ttgtgcaaga tgcacggcat tatagcacgt taccggcggc
     4801 ttgacagttt atattgtttg caatatcccc cgccttcagg cggcatacgg cgcggcggcg
     4861 gaatgccgtc tgaaggcggg cgttatgata attgttccag cagcgtctgc ttcaatgagg
     4921 actggatttt tgggtttttc aggtctaggc taaaaacggt aaaactgtct tcggcgcgtt
     4981 catccagtgt ggagattttg gcatagcgca ggctgacgtt gtgggcgaaa aagacttccg
     5041 ccatatcggc gagcaggaag gggcggttga cggcggtgat ttcgacggag taccaatcgg
     5101 ggtagtcttc ttcgggggtg atggtgatgc ttggcgcgat cggcatatag cggctgcggc
     5161 ggctgatgcg gcggctatgg ctggagattt cggcaacggt gtgtccgtgg ataaagctgt
     5221 tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggagcg tgctgcgagg
     5281 ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgataaag gcgcgggcgg
     5341 cgaggatatc gaagccgtgg cggctgaaaa tgcggcagag gcgggcgaac aggcgcgggc
     5401 cgttgggcat gaaaaccatg acttgaaagc tgttgctttt gggcaggatg cggctgcgga
     5461 cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg ccacaggatt tcgcgggact
     5521 ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag ttttttctgc tgtttttcgg
     5581 ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg gcggcggccg aagagggtgt
     5641 gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc atggaagagg ctttccagca
     5701 ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca
     5761 gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaag gcatcgagta
     5821 cgctggggtc ttggatgtct tctttttggg cgacggtgga cataagaaga tggttttcaa
     5881 ccagccaggc gagcaggtcg ctttcttctt cggtcaggaa gtggtcggcg gcaaattggc
     5941 gcgcgtctgc gatgccttgt atggcatggt cgccgccgcg tcctttggcg atgtcatgga
     6001 aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt
     6061 agggcagctc gtggctgtgc atatccaagg caaggcggcg gacgttgcgg acgacggtga
     6121 ggatgtggtc gtccacggga tagatgtgga acaggtcgtg ctggagcagg ccgacgattt
     6181 tttcccacgc gggcaggtag cgtcccaata cgccgtagag gttgaggaag cgcagggttt
     6241 gggtcagtcc gttgccgttg cggaaaaagc cggcgaagcg gtggcggttt tcagaatttt
     6301 ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca tgcgcgcagg gtttgcggtt
     6361 cgagcgcggt aatatcgttg cgctgctgca tgatttcgac gattttgaaa atgtgttcgg
     6421 gccgtctgaa aaaaatatcg gtgtgccgcg cggcaatttg gttgttgact tggatgtagt
     6481 cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga aacgcggctt tgcagcatag
     6541 gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca
     6601 gttcttcgct ttggcggcgg aggttcaagc cttcataacc catgctttcg gcgacttgcg
     6661 gctgcaaatc gaacagcagg cggtcttcgg cgcgcttggc gtttaaatgc agatggatgc
     6721 ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa
     6781 tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa
     6841 gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc tccaatactg
     6901 cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccagtttt gcctcgacaa
     6961 acgccgccac attgcgctgc gcgttcattt ttttcgccag ttcgtccgcc gtttggcggt
     7021 tgccgaacaa aaaacgggcc tctaaaaacg ccgtgtcccc cgtaatatcg tcgcgcacgc
     7081 tttcgcacag ttcgccaatg ccgccgcttt ttacagacgg catcagtttg cagtcccata
     7141 gggtttgaac aaaccgggca atctgttcct gaatgccgtc tgaaagcggg gcaggggaga
     7201 caaccgccaa atccacatcc gaacagggat acagttcgcc gcgcccgaag ccgcccaccg
     7261 ccatcaggca taacgcgctg ttttgaaaat actctgccca caatgccgcc agtaaggttt
     7321 cgactgccgc cgtgtattct ctgaaaaata ccgacacgcg gttggctttc aaataatgcg
     7381 cttcggcggc atcgcgctgc tgtttgaagg tttccagtgc tgaagacagg tttgcaggca
     7441 tttttattct ttcgattggc gggaaaaaag gagacggatg gttcggcggt caaataccgc
     7501 tttcagacgg catttgtcgg gtatcggcgg aattggtcat caggttcaac cgttcctgcg
     7561 gcggcagcag caactgatac gccgccacgc ccaaagccgc cgccattttt tcgatattcg
     7621 acagggcgat gttccagcgt ttgcgttcga ctgccgacac ataagtcctg tccaaaccgc
     7681 attgccgcgc caattcttct tgcgaccaac ccttgttcac gcggaaaagc cgcatattgt
     7741 atgccaatac cgcccgcaaa tcctgttcgt caggcagttc ggcaggcaga gtcaatttgt
     7801 tgcccatcat ttgcttccaa ataaatggtt aaagttaagc atttgctgtt acggatttta
     7861 cttcacataa aagccaaaaa aaaggggtgg cggcagccac acccaaacac acacacatca
     7921 agaggaaaga ggtaacatca agacagattg ggaaacagca aacaagcaat ctagatttca
     7981 tatcggtatt atcaaacaaa aaccgttatc gagtattgat tcaaatcaat ttagatttga
     8041 ttcgcaaacc ggcaaagaaa aacggcatac ccgtttatac ggatatgccg tctgatgatg
     8101 caggggactg ctgtatttgg cagtggtttg tgttttagtc ctcttgcgcc gctgtttgca
     8161 ggtagttcgg caaaccaatt ttgccgatta gttcctgctg ggtttcgagc cagtcgatgt
     8221 gttcttcgtt ggtgtctttt tgtttttcca acaaatcacg gctgacataa tcctgttgcg
     8281 cttctgctgt ggcgatggcg gcaagcaggg cttcgtgttt ttcctgttct ttggtcaaat
     8341 cgcaggcaat gatttcttcg gtggactcgc caatcagaag cttgcccagt tcttgcaggt
     8401 tcggcaggcc ttcgaggaac agaatgcgct cgatcaaatc gtcagcagct ttcatttcaa
     8461 cgatggactg tttgaagaaa tgttcgccca gttcttcaaa gccccagttt ttcaaaatac
     8521 gggcgtgaag gaaatattgg ttgatggtta ccagcagcaa gcctaagttt ttgtttagct
     8581 cgcggataac cagacggtcg cctttcatac ggactccttt tattccgaat acggattaca
     8641 gttggctttg gtagtagttg ccttcgccaa ttaactcgat caggcgaagc tgctgttcca
     8701 gccagtgggc gtggtcttct tcggtatctt tcagttgggc aaccatcagg tcgcgcgtaa
     8761 catagtcttg agcctcttcg cacagtttga tgcctttttt caacgcatca cgtacttcat
     8821 attcggtttg caggtcggct ttgaggcagg aaaccacgtc cgtgccgata ttcagttcgg
     8881 cgcgtgccat tttcggcgtg ccgcccagca tcaggatgcg gcggatgaag tcttcggcgt
     8941 gtgtggtttc ttcttccatc tcgtgattga gacgttcaaa aagtttggtg tagccccatt
     9001 cggagtagag gcgggagtgg ataaagtatt ggtcgcgtgc cgccagctcg ccagacagca
     9061 attcgttcat ataatcaaca acagcttgat tgccttgcat aatatctctc tttccgtaac
     9121 ttggttttcg gtatgtctga acagcatctg tgtcgctatt cgggatgcgt gcattgtatg
     9181 caaaagtttt tgatatgaca aattttctat ttaaaataca aacaattatc aaaagaaata
     9241 gggcgtattc cccgatgcct tccaaatcag tatgctgttt cttatcggtt tttttgctgc
     9301 taaaactaag aatccatctc atccataaag aacatttaca cttattaacc atagcaaaaa
     9361 acaaatagag cacggttttt tatcaaaatt tataatgaat cgttctcatt aaccgacaga
     9421 ttcttttgag attatcggat tttgggaata aattatgccg tctgaggggc tttcagacgg
     9481 catagacaat taaagctgat taaacaacgc ggcagacagc agcgtaggaa attatttttt
     9541 acttcggaac ggtattttgg gtttaatgat gcccgcaacc gccgtggctt tcgcgtaagg
     9601 cttcggcagc ctgttcgtcg gcgtggtagc tggaacgtac catcgcgccg atggcggcat
     9661 tgctgaagcc cagttcgtat gcttcttttt caaagatttt gaactgctcg ggcgtaacgt
     9721 agcgcaggac gggcaggtgt ccgtctgaag gctggaggta ctgtccgatg gtaatcattt
     9781 cgatattgtg cgcccgcata tcgcgcataa tttcacgcac gtcttcgtct gtttcgccca
     9841 agccgaccat gatgccggat ttggtcggga tgtgcggcat catttcttta taacgtttta
     9901 ataagtctaa agaatgttga taattggcac cgggacgggc ttttctgtac aggctcggat
     9961 gggtttctag gttgtggttc atcacgtcgg gcggggtttc ggcaaggatt ttgagtgcga
    10021 tgtccaagcg tcctcggaag tcggggacga ggatttcgat tttggtgttc gggctggttt
    10081 cgcggatggc tttgatgcag tcggcgaaat gctgtgcgcc gccgtcgcgc aggtcgtcgc
    10141 ggtcgacgga ggtgatgacg acgtaacgca ggttcatggc tttgacggat tcggcgaggt
    10201 ttttcggttc gtcggggtcg agcatattcg ggcggccgtg tcccacgtcg cagaacgggc
    10261 agcggcgggt gcagatgtcg cccataatca tgaaggtcgc cgtgcctttg ctgaagcatt
    10321 cgccgatgtt ggggcaggag gcttcctcgc aaacggtgtg cattttttgt tcgcgcaaaa
    10381 tgtctttgat ttcaaagaat ttgcgcgatg gaagtttggc gcgtatccat tcgggctttt
    10441 ttaatttttc ctgaaggggg acgactttga tggggatgcg ggcggttttg tccgcgcctc
    10501 tgagtttgat gccgcgtttg gggtcgtcgg ttttgatttc actcattgtt gtctgctttc
    10561 ggtgtgaatt gtgtttcaag gtgtgcggtg agtttggcgg cgacttcgtc cggcgtgggg
    10621 cagggttgga caaaatccgc gatttgcgtc atttccatac cggcgtagcc gcaggggttg
    10681 atgtgggtaa acgggcttaa atccatattg acgttgagcg caagcccgtg atagacggag
    10741 ccgtttttga tacgcagccc cagtgaggcg attttgcgtt ctccgacata aacgccgggg
    10801 cgtttggggt ctgccgccgc ttcgatgccg tattctgcca atgtggcgat gatgctgttt
    10861 tcaagcgcgg aaacgatgtt gcggacgctg gttttgcgcc gtttgaaatc aatcatcgta
    10921 taaacgacca attgcccggg cccgtgatag gtaatctgcc cgccccggtc gatttggacg
    10981 acgggaatgt cgtcgcgaat cagcaggtgt tcgggttttc ccgccagtcc ttgtgtgaac
    11041 acgggcgggt gttcgacgac ccacagttcg tcttcggtgt cggcattccg tccggcatta
    11101 aaggttttca tcgcttcaaa agtcggcaga tattcgacca aacctttgtg tatgattttc
    11161 atctcaaagt accactttga ccagttcgtg cgaagtcagc gcacggtaga tgttgtccaa
    11221 ttgttcttgg ttttcaacct ttacctgtac ggtggcgcca gtatagttgc ctttgctgct
    11281 cggacgcgtg gtgatgtggt gcgcctgcgt gtcgggggcg tgaaggcgga cggtgtccaa
    11341 taccgcctgc tcgaactcgg gatgcaccgc gcccattact ttcaatggga aggtgcaggg
    11401 aaattcgatg agggatgttt tgttttcttg ttcggtcatg atgtgctgcc ttgtcgtgta
    11461 cggtatgccg tctgaaggcg ggtttgcctt tcagacggca tcggatgtgc gttattttag
    11521 cctaaaccgc gataacaggc tatcgggaag gcggaggctt ttttgacggc gcggcggttc
    11581 tgctatactg gcgcacaata ttatttttag aagggtggtt tttatgtatc ggaggaaagg
    11641 gcggggcatc aagccgtgga tgggtgccgg tgcggcgttt gccgccttgg tctggctggt
    11701 tttcgcgctc ggcgatactt tgactccgtt tgcggttgcg gcggtgctgg cgtatgtatt
    11761 ggaccctttg gtcgaatggt tgcagaaaaa gggtttgaac cgtgcatccg cttcgatgtc
    11821 tgtgatggtg ttttccttga ttttgttgtt tgcattattg ttgattattg tccctatgct
    11881 ggtcgggcag ttcaacaatt tggcatcgcg cctgccccaa ttaatcggtt ttatgcagaa
    11941 cacgctgctg ccgtggttga aaaatacaat cggcggatat gtggaaatcg atcaggcatc
    12001 tattattgcg tggcttcagg cgcatacggg cgagttgagc aacgcgctta aggcgtggtt
    12061 tcccgttttg atgaggcagg gcggcaatat tgtcagcagt atcggcaacc tgctgctgct
    12121 tcccttgctg ctttactatt tcctgctgga ttggcagcgg tggtcgtgcg gcattgccaa
    12181 actggttccg aggcgttttg ccggtgctta tacgcgcatt acaggcaatt tgaacgaggt
    12241 attgggcgaa tttttgcgcg ggcagcttct ggtgatgctg attatgggtt tggtttacgg
    12301 cttggggttg gtgctggtcg ggctggattc ggggtttgca atcggtatgg ttgccggtat
    12361 tttggttttt gttccctatt tgggcgcgtt tacaggactg ctgctggcaa ccgtcgccgc
    12421 cttgctccag ttcggttcgt ggaacggcat cttggctgtt tgggcggttt ttgccgtagg
    12481 acagtttctc gaaagttttt tcattacgcc gaaaatcgtg ggagaccgta tcggcctgtc
    12541 gccgttttgg gttatctttt cgctgatggc gttcgggcag ctgatgggct ttgtcggaat
    12601 gttggccgga ttgcctttgg ccgccgtaac cttggtcttg cttcgcgagg gcgtgcagaa
    12661 atattttgcc ggcagttttt accggggcag gtaggcggtt ccgaaacata tttgaagcgg
    12721 aatacaacct tgcccgggtt taaataaaaa tgccgtctga accccgaaaa cgggacttca
    12781 gacggcattt tcatcacggc ttatttggcg gttttgctgc tgtcgataat tttcataccg
    12841 gcagaaatca ggctgccgat gtcggcaaca ttggcgggca taatcagcgt attgctttct
    12901 ttggcaagat tgttgaacgc ggcgacgtat tgttccgcaa tcttcagatt gaccgcatcc
    12961 gcaccgcctt gggtttgaag ggcggcggca atttgacgga tggcttcggc attggcttcg
    13021 gcaacaaggc gcaaggattc cgcttcacct ttggcgcggt tgatgcgggc gattttctcg
    13081 gcatttgacg cattgaccgc agcctgagcc tcgccttcgg attgttggat ttcggcttcg
    13141 cgctgaccac tggcaaggtt gatttgttcg attttacgac cttcggattc ggcgatacgg
    13201 gcgcgttttt cgcgttcagc agtaatttgc gcctgcattg agcgaaggat ttcttgcggc
    13261 ggaaccaagt ctttaatctc ataacgcaaa accttcacac cccaagctcc ggcggcttca
    13321 tcgagggcgg agacgacggt gctgttgatt tcgtcgcgtt cttcaaacgt tttgtccaat
    13381 tccatacgcc cgataacgga acgcagcgtc gtttgggcaa gctgggtaat cgccataatg
    13441 tagttgctcg aaccgtatga ggcgagtttg gggtcggtta cttggaaata gatgataccg
    13501 tcaacagtca gctgcgtatt gtcgcgcgtg atgcagacct ggctgggtac gtctaaaggg
    13561 atttctttca gcgaatggcg gtaggcgacg cggtcgataa agggaatcaa aatattcaaa
    13621 ccggccgtca gggcgcgatg gaaacgcccg agcctttcga caacgtggac ttcctgctgt
    13681 gggatgacaa caaaggattt gaagccgaaa acaacgacgg ctgccagcaa gataatgaaa
    13741 aattccataa ttcctccgag tgttaaggtt ttgcgatgat aagaaggttg ccttccttgc
    13801 ggacgattag ggcgcgcgtt cctggttcaa gctcttcttg ccccgtattt tgagcctgcc
    13861 agtgcgtacc gcgataaaaa acttcgtaac ggttgccgcc tgcgtgccgg aggatttcgg
    13921 catattgccc ggcatccaaa tcctgatatg aatccgtttc aacttttccc acggcggttt
    13981 tggcgtgtac gaaccaaata cccagcgcgg aaagcagagc ggcggtcaag acggcggcag
    14041 gcgtgctgcc ggtcagcccg taagcaatgc ccgaacccgc caaagccgcg ctgacaacca
    14101 aaagataaac cgttcccgtc aataattcga tgattaagac ggcaacagcg gcaacaaacc
    14161 atacagtcat acatttcccc acaaagcgcg tcgtttgaca aaatcacgcg atatcagcag
    14221 tatagccgaa tttgaaagga tagggcagat atggacactt ggcacgatgc actcggcggc
    14281 gaaaaacagc agccgtattt tcaggaaatt ttaaatgcag tcaggcagga acgtttgtcg
    14341 ggacaaatca tctatccgcc ggcggcggat gtgttcaacg cattccgcct gacagcgttc
    14401 gaccgggtca aagccgtcat tctcggacaa gatccgtatc acggggcagg gcaggcgcac
    14461 ggtttggcat tttccgtcag gcagggcgtc cgcatacccc cgtctttact caatatctac
    14521 aaagaattgg aaaccgacat cgaaggcttt tccattcccg cgcacggctg cctgacagcg
    14581 tgggcggagc agggcgtatt acttctgaac acggttttga cggtgcgtgc aggacaggcg
    14641 cattcgcacg cccttttagg ctgggaacgc tttaccgata ccgtcatcag gcagcttgcg
    14701 acacaccgca agcatcttgt ctttatgctg tggggtgggt atgcacaaca aaaaaggaaa
    14761 ctgatagaca gtcaaaacca tttgatattg accgcaccgc atccgtctcc tctgtcggca
    14821 tatcgcggtt ttttcggttg ccgccatttt tcacaggcaa acagctattt gagccggcac
    14881 ggtatcgatc cgataaactg gaagctgtga atgccgatat agccgttgcc gccggcgtgt
    14941 taaaatcgcg tttgatttgt aatttccatt tattaggcaa aaccttatgg caatcaaaaa
    15001 aaatcagctt tatgcatcac tttgggcggg ctgcgacgag ttgcgcggcg gtatggatgc
    15061 gagcctttat aaagaactat gtgcttacgc tattgttttt aaaatatgtt tctgataagc
    15121 ataagtacgg cggcggcatg attgagctgc acgccggtac gacttttgac gacatcgtca
    15181 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
    15241 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
    15301 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
    15361 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
    15421 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
    15481 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
    15541 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
    15601 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
    15661 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
    15721 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
    15781 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
    15841 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
    15901 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
    15961 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
    16021 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagc ggcggcagcg
    16081 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
    16141 agcaagacat tcacaaaatc atcgacactt tcacaaacct cgttacagcc gtatggtgca
    16201 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
    16261 agaagtcgaa gacctgcaaa atctcgccgc ctatctatct ttatggcggc atacctgcgc
    16321 acgatatgga cgcattggaa gcctattggc aagttttagg ccgtatgaaa aacgagttgt
    16381 ttgccgaaca cgatggccac tttaccacta tacaacggaa tcgattgcaa atctttccca
    16441 ctctcaacag cttaaaatcc tgcgggattg gtgtggaatt tagggctaat ctagtacagc
    16501 cccaaattta atccactata aaatcgaaag cagccaaatc aaagcccata tattggcgca
    16561 ccccgattac gccgccttca aagccggaca cctggcaaag tttgccgcgt ggcacactca
    16621 aaacgacctt gccgccatcc aaccgggcag gcttatccgg aaatggagcg aaagcctgct
    16681 ggacgcgttc aaacccggca gcctgattga agaatacgat ttctaccaaa tcctgacgga
    16741 ctactgggcg gaaaccctgc aagacgatgt ttatctcatc gcccaagatg gctggaaggc
    16801 ggttaaaaac ctggccgaaa tcaccaaaga aagcgatgaa gccgcgaacc tgaccgtcgt
    16861 ctttgaggaa accgaaaccg acaaaaaagg caaagccaaa accaagcgca tcagcaaaaa
    16921 ataccgcagc gaagtcatcg cccccgagct ggttgcccgc cgctactttt cagacggcat
    16981 cgccaagctg gaagaaaaac aaagcgagct ggaacgccta agccaagaat tggaaaacca
    17041 catagaagaa cacggcggcg aagagggtgc gctgaacgac gtattggatg caaaaggcaa
    17101 actttccgcc aaacttctga aaaccgcatt ggaagaaagc ggcatagaag aaggcgaacg
    17161 ggctgtttta caaaccaccc aaacactgat gacgcaggaa aaagccgcga aagacgcagt
    17221 caaaacccaa atcgaagccc tgaaccttgc cgtattcaaa caattcggcc gactttccga
    17281 agccgaaatc aagcagcttg ccgttcaaga caaatggctt gccgatttac aaagtcgaat
    17341 cgaaaatcgc ttggaaaaca gtattcagca gcttatcagc cgcttgaaca cgctggaaga
    17401 ccgctaccgc agcccgatgg ccgagcttgc ccgagaagtg gaaaagtggc aaagcaaagt
    17461 caatgcccac cttgaaaata tgggttttgg aggctgaaat ggcggcacag acaggctata
    17521 aggcgagcgg gttttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
    17581 acatttaggg gatcgcggtt cgggtgtccg caccgcttaa tacgtcgtcg tccacgaact
    17641 gacccatttg ctcgaacgcc atcgcaacgc ccgttttatg gcgcatatgg acaactttct
    17701 cccaaactgg caaagcatca aacaacagct taatgccttg gagttatttg cacaaatata
    17761 taatttaaca taatatacat tatgcgaact atcggaaaca gttgtacgtg tccctgtggt
    17821 ctttccaagt aggaaaatta aagtatggac aatgcggctg aatgtatagc ccggagcatc
    17881 cgcgtatccg aaggtactga acgatatgcc ctgcatccct ttcgatgctt ccatccagcc
    17941 ggtattggga aatacgttga ccttcaatat aatatacagg cagccgacac acaggatatg
    18001 ccgctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
    18061 ctttacatac gtcccaattc tctttgcagt gcttggatat tttgctgcct gtccaataca
    18121 ttgctttgta atgcatttat ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct
    18181 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cgttgctcaa ttctgcttcg
    18241 agaatggagc gtctgctgtt gtttacaggt gcttgttgag gcgggggcgt attggatttt
    18301 gctggcttgg atactgtttt gaccggggct ttatatttga caacctgtcc gccgtttgac
    18361 ggtgatgata ccggttcggg cgtttggggc gggatatagc gttcgctgct gtagttgccg
    18421 attgggggca aatcggttga gtggcagctt ttggacggct tggtggtgta aacggtttct
    18481 ccgttgattg tgcaggtgta gattttggcc gcattcgcac ccaatgggct tgaaatcagg
    18541 gaaaagttga ttaggattaa ggggagtttt gatttcataa tgtgcttgat gctcggataa
    18601 ttatttgatt ttttggtatt ttttataatg ctttcttggc gttttacggg cttttctttt
    18661 cggttgtttg cttttgattt tctttcgggg ctttggcagc ctgcttgttc tttttcccgg
    18721 caggtttttt gtttttcttt ttactttcta tgtgtatttt ggcttcttaa ctgagttttt
    18781 taattttcag gcggtatccg cctccctgat ggctgctgat tttaggtaaa tccgcatcgg
    18841 cgcacgatcc tgctggggct gatgtatata cgcttttgcc atttgagttg caatggtata
    18901 cggaggcttg cgccgccgcg ccggaaaggg acaggagacc caaggcggca aaaagtctga
    18961 tgtttatacc ggtaaaaggc ggtgataacg cccgaattat accgttattg gcaggcaaag
    19021 ataagcaccc tgtccgcgct tctttatcgc tcggcaaact gtttctcgat ttcagtttca
    19081 atccgttcca attcttcttg acaagccagc caaacctctt cgatttgggc aagttgtgtt
    19141 ttgacttttg tcagcaggga taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa
    19201 gcgtcttctt gtgctaaaaa tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt
    19261 tctttttcgg cacggtctat tttctgctgt atcggcttgc cgcgtcgggc tttttcctga
    19321 cggatttgcg cttcgatgcg cttggtgtct ttgcggcttt ggctttgtgc ggatgctgcg
    19381 ggtgcggcgg cggcgttttc ctgtgccaaa cgccattggc ggtaatcgtt caaatcgccg
    19441 tcgaagttct tcagacggcc tttgtcgatc aggaggaagc tgtcggtagt cgcttcaagc
    19501 aggctgcggt cgtgcgatac ggcgattaat gcgccttgga aactttgcaa cgcgagcgtt
    19561 aaggcgtggc gcatatccaa gtccaaatgg ttggtcggtt cgtcaagcag caacaggttc
    19621 ggcttttgcc agataatcat ggcaagggca aggcgggctt tttctccgcc ggaaaaaggt
    19681 tcggttttct gtaacgccat atcgccaaca aaattgaagc cgccgaggaa atttcggatt
    19741 tcctgttcgc gcacttcggg cgaaagctgc tgaatatgcc aaacagggtt ttggtcggcg
    19801 cggatggtat cgagttggtg ttgggcaaaa tagccgatat tgagtttttc ggaacggatg
    19861 atgctgccgg aaagcaaatc gattttgcct gccaaggctt tgataaaggt ggatttgccg
    19921 ctgccgttga cacccaatag cccgtagcgt gcgccgcttt ccagcgacag ggtaatgtca
    19981 tgcagaacgg ttttgccttc gtaacccaaa tcagcgtgtt ctagctttaa caaaggattg
    20041 ggcagatggt cgggatggta aaactcaaag gaaaactcgc tgtccagatg cgcgggcgca
    20101 acgcgttcta gctttgccaa agccttcatg cggctttgcg cttgaacggc tttggtggct
    20161 ttggctttga agcggtcgat aaaggattgc agatgtttga tttgcgcctg ctgtttgaca
    20221 taggcagctt gttgttgcgc gagacgctgc gcgcgttcgt tttggtaaaa atcgtaattg
    20281 ccgccgtatt gcgtgagctt ttgctgggac aattcgatgg tttgggtggt tgccgcattg
    20341 aggaaatcgc ggtcgtggga aatgatgatt tgcgtgcagg gtaaagaggc aaggtggttt
    20401 tccagccaca agacggtttc caaatccaag tggttggtcg gttcgtcaag caagagcaaa
    20461 tcggcgcggc aaatcagggc ttgcgcaaga ttcaggcgca tacgccagcc gccggaaaag
    20521 gatttgacgg gacggctgtg ttcttcttgc gaaaaaccca gcccgttcaa caatttcgct
    20581 gcacgggccg gcgcggtata agcgtcgatt tcttccaatt tagcatgata ttccgcctgc
    20641 ttcatgccgt cattttgcgc ttccgcctgc ctcaatgccg tctgaaaagc ctgcaactcg
    20701 gcatcgccct gcaaaacgta atccaaagcg gaaatatcca aatcgggcgt ttcttgggaa
    20761 acggaagcga gccgccagtt tttcggaatc gagacatcgc cgccgtcctg agtgatttta
    20821 cccttgatta aggcaaacag gctcgatttg cccgttccgt ttttgccgat caaaccgacg
    20881 cgctgaccgg gattgacggt agcgttggct ttgtcgagca ggactttcaa accgcgttgc
    20941 agggtgaggt ttttgatttc aatcataacg gaaacatcgt cgggcggaaa aagcccgtat
    21001 tttacctgaa agtcagtgcc gatgccgtct gaaatgggaa atttacggct gaagccaagc
    21061 ccaagccctg cgcccttccg agtgcaggaa aaccaatgtc ctgaatgccg aatcggtatt
    21121 catgcattcc acgctgattc cgattcggga aaaatccgcc atgattttgg gatggataaa
    21181 ctcctgagcc gcgcccgtcc cgataatcaa tatttccgga tagtcaacag gtttgatgtc
    21241 ggacaacagg ttttccggag tcagatcgga caaggttcgg cattgcgaca ggcagaccga
    21301 atccttatgt acaagcacgg gtttatggaa actttgcccc gtcagccgga ttccgcccgc
    21361 accgcattca tattccgcaa actgtccgtc tatcggattt tcttcaaaca acattttttt
    21421 accccgtcgc cgcatcatct acaccgaaag gggtgcaaaa tcagacaaat tcatgtagga
    21481 ttggcagatt tcatctgacc cgcctgccga tttcagacgg catttgattc aaagtgcggc
    21541 acaattatat cggcagcgga tattttcgtc tttcaatatt tacatttcag tcggcttaca
    21601 aggagacaca atgaagccag taaacatcgg tcttttaggt ttgggtacgg tcggcggcgg
    21661 tacggctgcc gtgttgcggg acaacgcgga ggaaatttcc cgccgcttgg ggcgcgaaat
    21721 ccgtatttct gccgtgtgcg atttgagtga agaaaaagcc cgacaaacct gcccgtccgc
    21781 agcctttgtc aaagatccgt tcgaactggt cgcacgtgaa gacgtcgatg tcgtcgtcga
    21841 attgttcggc ggtaccggca ttgccaaaga tgcggtgttg aaagccattg aaaacggcaa
    21901 acacatcgtt accgccaaca aaaaactgct cgccgaatac ggcaacgaaa tcttcccgct
    21961 ggcggaaaaa caaaacgtca tcgtccaatt tgaagcggca gtagcgggcg gtatcccaat
    22021 catcaaagcc ctgcgcgaag gtttggcggc aaacaggatt aaatccatcg ccggcattat
    22081 taacggcacc agcaacttca tcctctccga aatgcgcgaa aaaggcagcg cgtttgccga
    22141 tgtactgaaa gaagcgcagg cattgggtta tgccgaagcc gatccgacct tcgacatcga
    22201 aggcaacgat gcgggccata aaatcaccat catgagcgca ctggcattcg gcacgccgat
    22261 gaacttttcc gcctgctacc tcgaaggcat cagcaaactc gacagccgcg acatcaaata
    22321 cgccgaagaa ttcggctacc gcatcaaact tctgggcatc acccgcaaaa ccggcaaagg
    22381 catcgagctg cgcgtccacc ctaccctgat tcccgaaagc cgcctcttgg caaacgtcaa
    22441 cggcgtgatg aacgccgtgc gcgtcaacgc cgatatggtt ggcgaaacct tatattacgg
    22501 cgcgggcgcg ggcgcattgc cgaccgcttc cgccgtggtt gccgatatca tcgacatcgc
    22561 ccgcctggtt gaagccgata ccgcccaccg cgtaccgcat ctggcgttcc aacccgcgca
    22621 agtccaagcg caaaccatcc tgcctatgga cgaaattacc agcagctact acctgcgcgt
    22681 ccaagccaaa gacgaaccgg gcacgctggg gcaaatcgcc gcgctgttgg cacaagaaaa
    22741 cgtgtccatc gaagcactga ttcaaaaagg cgtgattgat cagaccactg ccgaaatcgt
    22801 gattctgacc cacagcacgg tcgaaaaaca catcaagtcg gcaatcgcag ccatcgaagc
    22861 actggattgc gtggaaaaac cgattaccat gatccgcatg gaaagcctgc atgactgagc
    22921 cgaaacacga aatgccgacg gaagagcagg ttgccgcgcg caaaaaagca aaagccaaaa
    22981 tccgcaccat ccgcatttgg gcgtgggtca ttttggcgtt gctcgcttta accgccctgc
    23041 tctcccaatg cgcgatgtcc aaaccgcagg caaaacagaa aattgtcgag tcttgcgtga
    23101 agaatattcc gtttgccgaa aaatggcaaa acgatttgcg ggcccgcggt ttagattcaa
    23161 acaatacccg cctcgccgtc gactactgca aatgtatgtg ggagcagcct ttggacagat
    23221 tgagcgagaa acagattaga tccttcggca aactcggcgc acaagaacag cttgacctgc
    23281 tcggcggcgc aaatgccttt gaagcacgtg acaagcagtg tgttgccgat ttgaaatcag
    23341 aataatgtgg accgataaaa aagccgattc tttaaagaat cggctttttt cataaaaaac
    23401 ggcttacagt gcgtctttca atgctttacc tgcacggaat ttaggcgttt tggcggcggc
    23461 aatggtcaga ggctcgccgg ttttggggtt gcggccttgg cgttccgcac gttcgcccac
    23521 gtagaaagta ccgaaaccga ccaaagtaac ggtgtcgcct tgtttcaggg cggtggttac
    23581 tgcattggta gtggcatcca aagctttttg tgcggcggct ttggaaatgt cggcttcttg
    23641 agcaatcgct tcgatcaatt cagacttgtt cacaatcagt cccttcctgt cttaaaaaat
    23701 gatgaaatgc ccgaatactc ggggtttgta ctgcttgagc aactttcgct ttatagcaat
    23761 tctgaaattg ccgtgtcaag caaaaaatac ggaatcaccc tatttgacag gctttcagga
    23821 cgaaaccgca tttttacaac acatttcctg cgtttcaatg tttggttgcc ctgctgcggg
    23881 gttttggttt tgaagcggat tccgccgccg cttccgcacc agaaggttct gcccaaggct
    23941 caggctggct ttccaaaccc agagccaata cctcgtctat ccatttgacc ggatggatgg
    24001 tcaggccggt tttcacgttt tcagggattt cttccaagtc tttgacgttg tctttcggaa
    24061 tcaggacgtg tttgatgccg ccgcgcaagg cggccaacag tttttccttc agaccgccaa
    24121 tcggcaggac ttcgccgcgc agggtaattt cgcccgtcat cgccacatcg gcgcgtaccg
    24181 ggattttgga aaatgcagac accatcgcca aagtcatggc aatgcccgca ctcggaccgt
    24241 ccttcggcgt tgcaccttcg ggaacatgga catggatgtc ttttttctcg taaaaatcag
    24301 aagccaagcc cactgattcc gcgcgggagc ggacaaccga ccacgctgca gacacggatt
    24361 ccttcatcac atcgcccaac tggccggtgc actgaatcac gcccttaccc ggcaatgctg
    24421 cggcttcgac ggtcagcaat tcgccgccga cttccgtcca cgccaaaccg gtaacctgcc
    24481 cgatacggtt ttcgctttcg gcaacgccgt aatcgaagcg gcgcacaccc aaatagtcgt
    24541 gcagattttt ctcatttact ttaaccgctt taggtttggc tttgctggtt ttcttggttt
    24601 cagacaacct cttcttatct tcgtccaagg taatctgcat caccaccttg cggcagattt
    24661 tggcaatctc gcggtcgagc gaacgcacgc ccgcctcgcg ggtgtagtaa cggataatat
    24721 cgcgcaccgc gctttcttcg acgagcaatt cgccttcttt cacgccgttg cgcttcattt
    24781 gcttcggcac gaggtactgc atcgcgatat tgattttttc gtcttcggtg tagccggaca
    24841 gacggatgat ttccatacgg tcgagcagcg gggtcgggat attcaggctg tttgaagtcg
    24901 caataaacat cacatcgctc aaatcgtaat ccacttcggc ataatgatcg gcgaacttgt
    24961 tgttttgctc gggatcaagc acttcgagca acgcgctggc gggatcgccc cggaagtcgc
    25021 tacccaattt gtcgatttcg tcgagcagga acaatgggtt ttttacgccg gcttttgcca
    25081 tattctgcag aatcttacca ggcatggagc cgatataggt gcggcggtgt ccccggattt
    25141 cgctttcgtc gcgcacgccg cccaaagcca tgcggacata tttccgcccc gttgctttgg
    25201 cgatggattc gcccaaagag gttttgccca cgcctggagg gccgaccagg cacagaatcg
    25261 ggcctttgag tttgtccata cgtttttgga cggcgaggta ttccaaaatc cgttctttga
    25321 ctttttccag gccgtagtgg tcggtatcca gcaccaatcc ggctttggcg atgtctttgc
    25381 tgacgcggga ttttttcttc cacggcagtt cgagcagggt gtcgatgtag ttgcgtacga
    25441 cggtggattc ggcagacatc ggcggcatca ttttgagttt tttcagttcg gacaggcatt
    25501 tttcttccgc ttctttgctc atccccgcct ttttgatgcc tgcttccaag gcatccagtt
    25561 cgccgttttc gtcttcttcg cccaattctt tgtgtatcgc tttaatctgt tcgttcagat
    25621 aatattcgcg ctgggatttt tccatttggc gtttgacgcg tccgcgtatg cgtttttcgg
    25681 cctgcataat gtcgagttcg gattccagct gtgccagcag gaattccatc cgtttgccga
    25741 tttcgggaat ttccaaaatc tgttggcgtt gcgccagttt caactgcaaa tgcgctgcga
    25801 ccgtatcggt tagccggctg ttttcggcaa tgccgttaat gctgccgata atttcggcgg
    25861 ggattttttt attgagtttg gcgtattgtt caaactgcgc caacagggtg cggcgcacgg
    25921 cttcgaggtc ggtattgccg cccgtgtctt cttccacgac cgcctctata tgggaaacga
    25981 acagaccgcc cgtgtcttca atggtcagaa cacgtccgcg atacagccct tcgaccaata
    26041 cttttaccgt gccgtcgggc agtttcaaca cttgcaggac ttgtgcgacc gtaccggtct
    26101 gatacaggtc ggcggcaacc ggttcttcta ccgccgcatc ggtttgcgcc aacaggaaaa
    26161 ccggctcctc gcgggtaatg gcgttttcca gtgcggcgat ggatttcggc ctgccgacaa
    26221 acagcggcag aaccatatgc gggtaaacga cgacatcccg caaaggaagg gttgccaagg
    26281 cggcatattc ctcaaaatgc ttttcttttt gtgtcatagg tactctcttg tgtctgacag
    26341 attgccgatt ttcgcgtaca ttggggttga aggtattatt tcaagcatat gtggtttatt
    26401 tatggagttt gatgcgatgc cgtctgaaac attccggctt cagacggcat gggcttggaa
    26461 agacaaggcg ggaacaaaaa actgttctgt gttgccgctc cttgctgtac catccgtatg
    26521 gtttgcggtt ctgccgccct attttcaaaa catgacgcag gtattccatg tcttctttta
    26581 tttttcccga taacgggttc gaatggcgca acgaaagtct gcaaaagccg cccaaaggtt
    26641 ggcattatgt ccgcgaaagc ggtgcaaacg gcattttgaa ggctgcctat caaaatattg
    26701 caactgtctg gcagggcgat ttccacaatg ccaaacaggt gctttccgca atgaagaaga
    26761 gggttctcaa acctgccgcc gtccgttccg atgcggatat cgccgccctt ttccacgccc
    26821 accgtatgaa gcaggcgcag cagagccgta ttctgaatat gcttgccgtt gaaatccgcc
    26881 ccggttttgt gttggacaac aaacgcgcgc ccgatatacg cgccgccctg ctcgacgtgt
    26941 acggagaggc ggacggcaaa ccgtttttcc tgccgctcaa tctgctgctg gggtttatgg
    27001 gtgcgcacga gtggcataag aaaggggttg ccgttccgca gctgggcggc agcatacacg
    27061 ttcctttcgg cgtattctcg ccgttgcgcg gcgaatacct cgacctgctc gcccatgcgc
    27121 cgtcaacggg ttttcagacg gcattagata tcgggacggg ctcgggcgtg cttgccgcca
    27181 ttttggcgaa acagggcatt ccttccgtca tcggcacgga taccaatccg aaagccgtcg
    27241 cctgcgcccg tgccaatatt gcccgtttgg gctttgaaaa acaggttgag atccgcgaaa
    27301 ccgatctgtt tcccgaaggg tttgtcgatc tgatcgtctg caatccgccc tggcttcccg
    27361 ccaagccgac ttccgccgtc gaatccgcgt tatacgaccc cgaatctgcg atgctggctg
    27421 cgtttttgcg ggatgcgccg aaacatctga atcccgacgg agaaatccgc ctgatcattt
    27481 ccgatcttgc cgaacatctg cacctgcgtc cgtccgattt tctggataag gcatttgctc
    27541 aggcgggttt gcgcgttgcg gatgttttga aaaccaaacc ggttcacaaa aaagctgccg
    27601 atccgaacaa tccgctggct tttgcccgaa acagggaaac tactttcctg taccgtttga
    27661 aaaaggcata aggggcggcg gcgcggcatt cgggcggatt attcttgtga aaatacccgc
    27721 tcgagcatac tgcccaatgc cgtctgacgc gttttgacag tggcggcagc ttgccggaag
    27781 gtttcttctc cgccgaagaa tacgaacttc tccctcgcgc gggtaatggc ggtatataac
    27841 agctccttac tcaatccgga caatgcatcg tccccttcgt ccgaaggtgc ggcggaaggc
    27901 ggcagcagcc agacttcccg atattccgaa ccttggcttt tgtggacggt catggcgaat
    27961 gcgggttcaa attcgggcag gcagcttacc gctacctttt taaatccgtc cgcatcggca
    28021 aaataggcgg caaggctgcc ctgccgtccg acatcttcca taatcagtcc gatgtcgccg
    28081 ttgaacagtt caagcgcgta gtcgttctgt ctgatcataa tcggttctcc ggcgaaatag
    28141 gcctgatgtt ccggtatgtt cattttgcgg cgtacatggc tgcaataggc ttcgttgaag
    28201 tcttccgcat cctgccgcca cgccgtgagg acgacgatat ccgagatgcc tgcgtatgcg
    28261 gcttcgatat tgccgtcttt taccgcctgc caataggctt tgtgcgcccg gtacaacctt
    28321 tcgactcgag cgttcggact gcattccgaa tgttccagtt catccggaaa ccggtcaaac
    28381 aatgcccacg ccccctcatc gcccgatacg gcggcacggg caaggcagcc gatgccgctg
    28441 ttgtcgccga agcggtggct gaacgacaga tgggcggtgt tgtgcgccaa cacgggcgga
    28501 tttgcgctga cgctgaaacc gtgttctgga aggaagccgg ccagcctttg gtgcgtttct
    28561 ccgtccaaaa cggttttttg cgacaaaacg gacagcaccg ccccgattcc gacggacggg
    28621 agctggtttt catcccccag cagaatcacg cgcgcgccgg ttttgaccgc ttttaaaagt
    28681 tgcaacatca atgcggtatc caacatggag gcttcatcga caatcagcac atcgaacggc
    28741 agcggacgga tatggtcgaa cgccgcctgc attttgggcg ggcgcagctt cagcaatcgg
    28801 tggacggttt gcccttccag tttgagcaaa tggcggcgga cggcctccgg cgcgtcaaaa
    28861 ccgttgattg cacggtgcag cgcgcgcgcc atatgtgctg ccgctttgcc cgtcggtgcg
    28921 gcaagcgcga tatggggaag attttcgtct tcaccgcaaa tcagcgccag cagtttggca
    28981 accgttgtcg ttttgcccgt tcccggcccg ccggtaatca ccataaaaga ctgtaacagt
    29041 gccaaggcgg cggcatcgcg ctgtccttcg ctgcccgcgc cttgaaacca ttttgcgagg
    29101 ttttgccgcg cacctgtcgc gtcgggggcg gatgtgccgg ctgccgccaa gcgttttatc
    29161 tcggcagcca aatcgtattc caactgccac atcctgccca aaaacagcct tctgccttcc
    29221 aaaatcaaag gcgcggcgga tgttccgacc acgggtgcga gtgccgacag cgcatcagcc
    29281 tcgccaccgc tcaaacggat aaacgaatga ccgttctgca atgcctgaaa caggcgttcg
    29341 gtacagtttg caagcacttc gtcgccagaa cccgcatagt gttccaaaaa acggattgcc
    29401 gcccttgccg ccgcttgggc aaattcatct gtctgccgtt ccatcgtatt ccttgtcgaa
    29461 atgccgtctg aaggcgtggg gcttcagacg gcgcggtgtt gtttcggctg tttaggcgtt
    29521 tgcgccctgt ttggggtgca ggctgccgtc tttcatgacc atcacgcgcc cgaagcggcc
    29581 ggcgagttcg tcgtcgtgcg ttacgaccac cagccccgtt cccaattctg ttttcagttc
    29641 cagcatcata tccaaaacat tccttgcgtt tgctcggtcg agattgccgg tcggttcgtc
    29701 ggcaagcagg catttgggtt gcgtaaccaa ggcgcgcgca atggcggcac gctgccgctc
    29761 accgcctgaa agttcgcccg cgcggtgcgt cgaacggtgt ttcagtccga ccttgtcgag
    29821 catcgacatt gccgtttctg ccgcctcttc acggcttttt ttgccgatca gaagcggcat
    29881 catcacgttt tccagtgccg aaaattcagg cagaagatgg tggaactggt acacgaaacc
    29941 gagatggcgg ttacgtaaat cgcccaaacg ccgctggttt aaggtacgca aatcctcgcc
    30001 catcagcagc accctgcctt cagacggcat atccagcccg cctagaatat gcagcagcgt
    30061 cgattttccg ctgcccgaag aaccgatgat gccagtgctt tcccccgcgc ggatttccaa
    30121 atccaagccg tgcagcaccc gaacgtccaa accgccgtcc cgatagcgtt tgcccacgcc
    30181 ttcgcatttc aaaatcaact cactcataac gcaaagcctc cgccggttgg gtttttgacg
    30241 cgcgccggct cgggtagagc gtcgccacga aagacagccc caaggaaatg caggcaatca
    30301 gggcaacgtc gcccatatcg acatcgctgg gcaggtagtc gataaaataa acctgcgaat
    30361 tgatgaggtg gacaccgagc aggttttcaa aaaacgccac gaccctgccg acgttccaac
    30421 ccaaaagcac gccgcagacc acacccgcca acgtgccgaa aaagcctgaa aacgcgccct
    30481 gcaccataaa aatcttcatc acgccgccag gggaaagacc caaagtccgt aaaatcgcaa
    30541 tgtccgcctg cttttccgta accgccatca ccaaggaaga gacaaggttg aacgccgcca
    30601 cggcgataat cagcgtcagg atgatgaaca tcatccgttt ttccaactcg accgcctcaa
    30661 aatagctgcg gttgctgaac gtccaatcgc gtacccaaac cgtatccctt tgcgcctccg
    30721 gaatcagctt tgccgtcaag gcgggggcgt tttgcgggtc ggcgagtttc agccgcagcc
    30781 ccgcaacttc cttatccaag cggtacagta cgcgcgcgtc ttggatatgc gtcatcgcca
    30841 atgagttgtc aacctcgtaa acacccgttt taaccagacc gaccacggta aactgtttca
    30901 acctcggtac gactccggcg ggcgtaacat tgccctccgg cgtgatgacg gtaactttat
    30961 tgccgacttc cgcccccaaa gcctccgcca agccgacacc gaggataatg tcaaactcgc
    31021 ccggaatcag gtcttcaaat ttgcctgccg gcattttgtc gccgtattcc accactttgc
    31081 gctcttcaga cggcaaaatg ccgcgtatct gcacgcccct gatttcgccc gcattggcca
    31141 gcaatgcctg attggaaaca tagggcgcgg cagccaaaat acctttgcgg ttttcggtaa
    31201 accgaagcag gttgcgccaa tccgtatccg tattgtcgat atagccgatt tcggcgtgcg
    31261 gcgcgacatt caggagctgc ccgcgtattt ctttctgaaa gccgttcata accgacaaga
    31321 cgacaatcag cgcggttacg cccaaggcga ttccggcaat cgaaaccatc gtgataaacg
    31381 acataaagcc gttgcgcttt ttcgccctga gatacctcaa gcctatccaa gcctctagag
    31441 aaaacataac gctaccttaa aaatgtctgc aaacgtgccg cccccggacg gcggtttggg
    31501 ggcgacggca aaagtttatt gtaccgtaaa acccaggcag cgtccgaacg cccgagtgcg
    31561 ggggacgggg cggcagagcg ggcggaaaac ttgcacaacg cgtcaaactg ccctatcctt
    31621 tccttcagaa aaaccgtttc ttgaggaaaa caatgaatat ccgaactgct tttgctttgt
    31681 gcgccatcgc cttatccgcc gctgcggctg cctacgccaa agaaatcaaa atcgatgcca
    31741 acaacacgcc ttattccgaa gccgacgcgc aaaagctggc ggcaacggca gtcggtatgg
    31801 gtgttaagga acctatcagc ctgaacggcg gcagcggcag cattaccgtg tccggcagca
    31861 gcgcgacgca gtgcgtgttc aaagtcggca acggaggcgc gttgcagatt caagggctaa
    31921 actgcaagta aaccgcccgg aaaaatgccg tctgaaggct tcagacggca ttttgcattg
    31981 gcggcgttat gccccgcctt ctttaatcag gcggcgttcg tacaccgcct gcgccagcgt
    32041 tcccgcatcg acatattcca attcgccgcc caagggaatg ccctgcgaca gcctgctgac
    32101 tttgtaaggc aggtttttaa aaaactcgga caggacatac gccgtcgcat tgccttctgc
    32161 ggtaaaagcg gttgcaataa tgatttcttc gacttccccg ccgcccagcc gttgcgccag
    32221 cctgtccaat gcgatggcgg atacgtccat tcccaatgcc gtattgattt gccccatcag
    32281 gacgaaatac agcccgtcgt ggcagtttgc cgcttccata ttcgacacgt cggcaggcat
    32341 atgcaccacc atcagccgtc gcccgtcgcg tgtttcatcg gcacaaatat cgcacaatcc
    32401 gccttcgcaa aacgtgttgc acctcgcgca atgatcgacc tgcttcaatg ccgtctgcaa
    32461 ggcatccacc agtttttcag cctctttgcg cttgtgttgg agcaaatgat acgctatccg
    32521 ctgtgccgat ttcggcccga cgttgggtaa aaccttcagc gcgtcgatca atccttggaa
    32581 ggcatcttgt tttttgtggc tcatcatatt ccgccgtatg ggaaaacggc cggaatattc
    32641 cgaccgttat tttgtcaaca aaagtgtcaa ttactgaccg tcgccgttgt cgaccgattg
    32701 cgctcctttg gtctgtttga ttttgccgtt gaaataacgt atcaacaagt cgaaagtatt
    32761 ggcagactgc tgttgcgcca aagcctgttt tgcaagcgga agctgtgcgg cgatatcatc
    32821 cggcggggtt acagcctgta cttcgacaat cacgggtgcc ggcagaccga tcagcctgac
    32881 gtaggcgggt ttgccgtttg ccggttttgc tttcagcagt tccgcataag cctcgggcgg
    32941 catggactgc cttgcctgct gtgcgcccaa aacggacact tccgaccatt tcacgtcaac
    33001 agccttgccg ccgttcagtt gggtaagcac gtcttttgcc ttgttttcgg caagtttggc
    33061 ggcttcggta cggatataag cctgacgtac cgcgtctttg gcttcggcaa acggcagggt
    33121 tttctcttcg cggacttctt tggcgcggac gacccacgcg gtttcgctgt tgatggtcag
    33181 cacttcggaa ttgtgttttt tcttcaatac gtcgtcgctg aatacggcat tgatcaggtt
    33241 ttcgggcata ccggacattt gcgcatcctg cctgctcagc caagtttctt gggtttcgac
    33301 tttcaaaccg ctgtttttgg cggcttcggc aagcgaggaa ggatggttga acgcatcatc
    33361 gcccagcttt tcttttgcct tattgaagtc ggcaaccgcc tttttcattt tcaattcgtt
    33421 ttcgacggcg gctttttcct gctcgaaaga aggtttggct tcatttgccg gcaaacgcgc
    33481 cacgcgctct tcaaacgcat ttttcacttc tgtttcgctg acggtctgtt tgtctgcaaa
    33541 gtctttcaga ttcaaggcga catattccaa tttgaccgct ttgggaagca ggtagtcttt
    33601 tttgtttgcg ttataaaact tctgcaaatc ggcttcagac actttgactt gggcgatgaa
    33661 ttcgtcgggg ttgaaagtgt gcgaacggat ggtgcggttg acctgcgtca gcctgatcag
    33721 ctgttccgcc tgcgcgtcgc cgaccaatac gccgttttgg acgaggttta ccaaattctg
    33781 caaggcaaac tgatcgcgga tttcttcgac aaactggtct tcagacatat gacgttggga
    33841 aaggtagcgg tttaaaagcg cgtggtcgaa tttgccgttt gcgtcgtgga aattgggatc
    33901 gtccacgata atctgcttga tttgttcgga agaaaccgaa atgcccatca gcttcgcgcc
    33961 ctgtttcagg taggcgcgtt gtagcaggga ttggaacacc gcgtcgcgcg aagggccgcc
    34021 gccgtccgcc tgttcgttct gtatggcgtt gttgatggag tggtcgctga ttttttcgtc
    34081 gcccacttgg acgatgtagt cggcacccgg atgggatacc gtgctgaccc cgaagccgac
    34141 gaaggttaat gcaatcaggc ccaaaaggac ttgggcgggc gttctgtatt tttcgatgga
    34201 atggaacata ttttaaatcg ggatatagaa tgggaaattc aagtcgggta ttgtaacggt
    34261 ttttatccct gtctgcacgg ggcttgccgg ttgaagatgc cgtcgtaggt ttcttcgctg
    34321 aagctggcgt aaaccttgcc gccgcactcc aatacgggac gcttgatcag gctcggcatt
    34381 tcggacatca gtttgacggc ctccgccgtc gaggacagca ctttttgctg tgtttcggca
    34441 tcgagtttgc gccagcttgt cccgcgtttg ttgagcaggg ttgccaaagg cacttgttcc
    34501 agccacgagc agatttccgc ttcagacggc ctctgttttt taaaatcccg aaattcaaac
    34561 tccaagccgt atccggcaag ccggtttttg gcttttttga ccgtatcgca atttgggatg
    34621 ccgtgaagga ttatcatttg gaaacctttt gtctgaaata ataaaacgga tattttacta
    34681 taagtgtctg aaaatttgcc cgtctgtttc agacggcggg gcggttatgt tacaatccga
    34741 aaattcgaaa aatttaatct cttgttcaat aaaggcttta ccaatcatga tttctaccaa
    34801 cggcatcacc atgcagttcg gcgcgaagcc gctgtttgaa aacgtatccg ttaaattcgg
    34861 cgaaggcaac cgctacggtt tgatcggcgc gaacggctca ggcaaatcca ccttcatgaa
    34921 aatcctcggc ggcgatttgg aacagacagc cggcgaagtg gcgattgaaa acggcgtgcg
    34981 tttgggtaaa ttgcgccaag accagtttgc ctacgaagat atgcgcgtgc tggacgtggt
    35041 aatgatgggt cacaccgaaa tgtgggcggc gatgaccgaa cgtgatgcga tttacgccaa
    35101 tcccgaagcc accgaagacg actacatgaa agccgcagaa ctggaagcca agttcgccga
    35161 atacgacggc tacaccgccg aagcgcgtgc cgccgagttg ttgagcggcg tgggcatttc
    35221 cgaagatttg cacaatgcaa ccatggcaga agttgccccg ggcttcaaac tgcgcgtatt
    35281 gctggcgcaa gccctgttct ccaagccgga tgtattgctc ttggacgaac cgaccaataa
    35341 cttggacatt aataccatcc gttggttgga aggcgtgttg aaccagtacg actccacgat
    35401 gattatcatc tcgcacgacc gccacttctt gaacgaagtc tgcacgcata tggcagattt
    35461 ggattacaac accatcacca tctatccggg caactacgac gactatatgc tcgcctccgc
    35521 ccaatcgcgc gaacgcgccc tgaaagacaa tgccaaggcg aaggaaaaat tgcaggaatt
    35581 gcaagaattt gtggcgcggt tcagtgccaa caaatccaaa gcccgtcagg caaccagccg
    35641 tctgaaacag gcagacaaaa tcaaatcgga gatggtcgag gtcaaaccct ctacccgtca
    35701 aaacccgtat atccgttttg aagccgatga aaaagccaag ctgcaccgtc aggctgtgga
    35761 agttgaaaaa ctggcgaaac gctttgaaac ccagttgttt aaaaacctga actttatcct
    35821 tgaagcggga caacgcctcg ccatcatcgg cccgaacggc gcgggcaaat ccaccctgct
    35881 gaaactcttg gccggcgcgt acaaccccga atattcagac ggtctgctgc cggacgaagg
    35941 cagcatcaaa tgggcggaaa aagccagcgt cggctactat ccgcaagatc acgaaaacga
    36001 cttcgatgtc gatatggatt tgagcgaatg gatgcgccaa tgggggcagg acggcgacga
    36061 cgaacaagtc atccgcggca ctttggggcg tttgctcttc ggcagtaacg atgtcgtgaa
    36121 aaaagtgaag gttctctccg gtggtgaaaa aggccgtatg ctttacggca aactgttgct
    36181 gttgaaaccc aatgtcttag tcatggatga accgaccaac cacatggaca tggaaagcat
    36241 cgaatccttg aacatggcgc tggaaaaata caacggcacg ctgattttcg tctcccacga
    36301 ccgtcagttc gtttcttccc tggctaccca aatcattgaa ttggacggca aaggcggata
    36361 tgaacactac ttgggcgatt acgaaagcta tctcgagaaa aaaggcgtag cataaccgcc
    36421 ggttggaaca atgccgtctg aagcggcttc agacggcatt gttgataact ttaaaatagg
    36481 aagcatatgc agacttatct cgtcggcggt gccgtccgcg attatctttt gggcttgccc
    36541 gtcaaagacc gcgattgggt ggtcgtcggc gcagacgcac aaaccatgct ggcgcaaggc
    36601 ttccagccag tcggcaaaga ctttcccgtg tttctccatc ccgaaacaca cgaagaatac
    36661 gccctcgccc gcaccgagcg caaaaccgcc aaaggttacg tcggtttcag tttccacgcc
    36721 gacaaagacg ttacgctgga gcaggatttg atgcgccgcg acctgaccat caacgcgatg
    36781 gcgcaagatg cggacggcaa gattatcgac cctttcggcg gacaacggga tttggcggca
    36841 ggcattttgc gccacgtttc cccagccttt gccgaagacc ccgtccgcat cctgcgtact
    36901 gcccgctttg ccgcgcgtta caagtttgaa atcgccgaag aaaccataaa gctgatgcgg
    36961 cagatggtgg aaaacggcga agcggacgca ttggttgccg aacgcgtctg gcaggagttt
    37021 gcgaaaggtt tgatggaaaa aaatccgcgc aaaatgattg aagtgttgcg cgaatgcggc
    37081 gcgctcaaag tcttgctgcc cgaagtcaat gccctcttcg gcgtgccgca acgcgccgac
    37141 taccatcccg aaatcgacag cggcatccat accctgatga cgctgcaacg cgccgccgat
    37201 atgggtttga gcctgcccga acgctatgcc gccctgctgc acgacttggg caaagccaaa
    37261 acaccgtccg acatcctgcc gcgccaccac ggacacgacc tcgccggtgt cgaacccgtg
    37321 cgcaaagtca atcaaaggct gcgtgcgccg aaacattgtg ccgagcttgc cgaattggtt
    37381 tgccgttggc acattatttt ccaccaagtc ggacagctta aaagccaaac cattctgaac
    37441 gtattgaaaa aaaccgacgc tttcagacga cccgaacgct ttcagacggc attgaacgtc
    37501 tgcattgccg acacgcaagg ccgtctgaac cgcgaacaca cgccctaccc gcaacgcgcg
    37561 cactggctcg ccttactcga agccgccaat caggcggatt cgggcaaaat cgccgccgaa
    37621 tgccgctcgc agggaaaagc gcaccttatc gccgaacaaa tcgaccgggc gcggctggca
    37681 caaatcgccc cattgcaaaa agcgtttcga gcggcgcaag acaaaacaga aaaacattaa
    37741 aacgtccaat gcagcca
//
LOCUS       DAWKOD010000004        32003 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000004 DAWKOD010000000
VERSION     DAWKOD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.004"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..939)
                     /locus_tag="WJK73_000128"
     CDS             complement(142..939)
                     /locus_tag="WJK73_000128"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ7556672.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1094..3943
                     /gene="uvrA"
                     /locus_tag="WJK73_000129"
     CDS             1094..3943
                     /gene="uvrA"
                     /locus_tag="WJK73_000129"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ7556673.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEQSITGQYLSSKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            3977..5572
                     /locus_tag="WJK73_000130"
     CDS             3977..5572
                     /locus_tag="WJK73_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ7556674.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6123..7013)
                     /gene="sucD"
                     /locus_tag="WJK73_000131"
     CDS             complement(6123..7013)
                     /gene="sucD"
                     /locus_tag="WJK73_000131"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ7556675.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7024..8190)
                     /gene="sucC"
                     /locus_tag="WJK73_000132"
     CDS             complement(7024..8190)
                     /gene="sucC"
                     /locus_tag="WJK73_000132"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ7556676.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8289..8576)
                     /locus_tag="WJK73_000133"
     CDS             complement(8289..8576)
                     /locus_tag="WJK73_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556677.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8671..10104)
                     /gene="lpdA"
                     /locus_tag="WJK73_000134"
     CDS             complement(8671..10104)
                     /gene="lpdA"
                     /locus_tag="WJK73_000134"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7556678.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNSDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(10432..11613)
                     /gene="odhB"
                     /locus_tag="WJK73_000135"
     CDS             complement(10432..11613)
                     /gene="odhB"
                     /locus_tag="WJK73_000135"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ7556679.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVTLPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(11713..14541)
                     /locus_tag="WJK73_000136"
     CDS             complement(11713..14541)
                     /locus_tag="WJK73_000136"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ7556680.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(14746..16029)
                     /gene="gltA"
                     /locus_tag="WJK73_000137"
     CDS             complement(14746..16029)
                     /gene="gltA"
                     /locus_tag="WJK73_000137"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7556681.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(16141..16389)
                     /locus_tag="WJK73_000138"
     CDS             complement(16141..16389)
                     /locus_tag="WJK73_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ7556682.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(16393..17100)
                     /locus_tag="WJK73_000139"
     CDS             complement(16393..17100)
                     /locus_tag="WJK73_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ7556683.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(17221..18984)
                     /gene="sdhA"
                     /locus_tag="WJK73_000140"
     CDS             complement(17221..18984)
                     /gene="sdhA"
                     /locus_tag="WJK73_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ7556684.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(18987..19328)
                     /gene="sdhD"
                     /locus_tag="WJK73_000141"
     CDS             complement(18987..19328)
                     /gene="sdhD"
                     /locus_tag="WJK73_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ7556685.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(19322..19699)
                     /gene="sdhC"
                     /locus_tag="WJK73_000142"
     CDS             complement(19322..19699)
                     /gene="sdhC"
                     /locus_tag="WJK73_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ7556686.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(19978..21381)
                     /locus_tag="WJK73_000143"
     CDS             complement(19978..21381)
                     /locus_tag="WJK73_000143"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7556687.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(21630..22367)
                     /locus_tag="WJK73_000144"
     CDS             complement(21630..22367)
                     /locus_tag="WJK73_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ7556688.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(22646..24922)
                     /gene="metE"
                     /locus_tag="WJK73_000145"
     CDS             complement(22646..24922)
                     /gene="metE"
                     /locus_tag="WJK73_000145"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ7556689.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(25060..25938)
                     /gene="metF"
                     /locus_tag="WJK73_000146"
     CDS             complement(25060..25938)
                     /gene="metF"
                     /locus_tag="WJK73_000146"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ7556690.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            26139..26414
                     /locus_tag="WJK73_000147"
     CDS             26139..26414
                     /locus_tag="WJK73_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ7556691.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            26414..26539
                     /gene="ykgO"
                     /locus_tag="WJK73_000148"
     CDS             26414..26539
                     /gene="ykgO"
                     /locus_tag="WJK73_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ7556692.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(26647..26847)
                     /locus_tag="WJK73_000149"
     CDS             complement(26647..26847)
                     /locus_tag="WJK73_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556693.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            26997..28136
                     /locus_tag="WJK73_000150"
     CDS             26997..28136
                     /locus_tag="WJK73_000150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ7556694.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            28133..28714
                     /gene="metW"
                     /locus_tag="WJK73_000151"
     CDS             28133..28714
                     /gene="metW"
                     /locus_tag="WJK73_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ7556695.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            28831..29676
                     /locus_tag="WJK73_000152"
     CDS             28831..29676
                     /locus_tag="WJK73_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ7556696.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(29726..30286)
                     /gene="efp"
                     /locus_tag="WJK73_000153"
     CDS             complement(29726..30286)
                     /gene="efp"
                     /locus_tag="WJK73_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ7556697.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(30329..31477)
                     /gene="earP"
                     /locus_tag="WJK73_000154"
     CDS             complement(30329..31477)
                     /gene="earP"
                     /locus_tag="WJK73_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ7556698.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(31496..31774)
                     /locus_tag="WJK73_000155"
     CDS             complement(31496..31774)
                     /locus_tag="WJK73_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556699.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         8048 a         8155 c         7831 g         7969 t
ORIGIN      
        1 tgataaatca ccgtaacgtt aaatttccag attcccattt tcgtgaacat aacggtgtga
       61 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
      121 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
      181 agccgccgct gcctcgattt gccgttgttc ggcagatggg tttgccgctg tttcaaccgg
      241 agcagtttgt aaagcaggct cagactcagg ctcggattcg atttgcggcg cagtcagcgg
      301 caaacgcgcc aatacagtgc taacgccagt tactttatcg ccaatcgcca cttgcgcctg
      361 cgcatcgaca ggcagataca tatccacgcg cgaaccgaag cggataaagc cataacgttc
      421 gccgcgggac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg ccaccaaacc
      481 ggccacttga acaaaagtaa tttcacgacc ggaagccgta gtcgccaaca ccgcattacg
      541 ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt tgttatagac
      601 cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga acacgttcat
      661 aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc gctcgaccac
      721 cacgatacgg ccgtcaaccg gactcaacac tgcttcagga ttttgcggaa tctcacgcgc
      781 agggtcgcgg aaaaactgca gggcaaatac ggtaaacacc caaaacggca aagaccacca
      841 gccgcaacac atcgacacca gcaggctcaa agccaaaccg ccgccaataa tcggccagcc
      901 ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt ttgttcaagc
      961 gggttaaaat tgccgccatt ataacggaat ccgccgcgtt ccgccgacaa atgccgtctg
     1021 aacgcggctt cagacggcat ccgagaaagt gctacaatac cagttttcct ttatttttca
     1081 ggcggcatcc attatgtaca agcatcatcc ccaacattcg cacgacaacg acaccatccg
     1141 catccgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttggacattc cgcgccacaa
     1201 gctcgtagtg gtaacaggct tatcgggcag cggcaaatcg tcgctggcat ttgatacttt
     1261 atatgccgaa ggtcaacgcc gttatgtcga gagcctttcc gcctatgcgc ggcagttttt
     1321 gcagatgatg gacaaacccg atgtcgattt gattgaaggt ctgtcgcctg cgatttctat
     1381 cgagcaaaaa tccaccagcc acaatccgcg ttccaccgtc ggcacggtta cggaaatcca
     1441 cgactacctg cgccttttgt acgcccgcgt cggcacgccg tattgccccg aacacaatct
     1501 gccgttgtcg agccaaaccg tatcgcagat ggtcgatgcc gtgttgaagc tgccggaaga
     1561 cacgcgcgtg atgattctgg cgccggcggt gcgcgagcgt aagggcgagt ttgtcgattt
     1621 ctttgccgac ttgcaggcgc agggttttgc gcgcgtgtgc gtggacggcg aggtctatca
     1681 gcttgacgaa gtgccgaagc tggaaaaaaa catcaagcac aacatcgacg tggtcatcga
     1741 ccgcgtgaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg aaaccgcgct
     1801 gcgccacggc aacgagcgcg cgctggcgat ggagatggac agcggcgaag aacattggtt
     1861 ttccgcgcgt ttcgcctgcc ccgtatgttc gtacagcctg cccgaattgg agccgcgcct
     1921 cttttcgttc aacaacccga tgggctcctg cccgacttgc gacggcttgg gtaacaccaa
     1981 tttcttcgac tccgaaaaag tggtcgcgca tcccgaattg tcgctggctt cgggcgcgat
     2041 tgacggctgg gacaagcgca accagttcta tttccaaatg attcagtcgc tggcacggca
     2101 ttacggtttc gacgtgcagg ctgcttggga aaccctacct gcaaaaatca aaaaagtcgt
     2161 gttgcacggc tcgggcaaag aagtcattga tttcacttac ctgtccgaac gcggcaccac
     2221 cttcaaccgc agccacgcct tcgaaggcat catccccaat ctcgaacgcc gctaccgcga
     2281 aaccgacagc gaaaccgtgc gcgaaaaact gcgcgaatac caaaaccacc gcgcttgccc
     2341 gagctgcggc ggcgcacgtt tgcgcaaaga agcccgctac gtttacgtcg gcggcgagcc
     2401 gttgcacgaa gtttccgcct ggccgctgac caaaacccac cgattctttg aaacgctgga
     2461 tttggacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatca ccgaacgtct
     2521 cggctttttg attaatgtcg ggctggatta cctgaatctc tcccgttccg ccgaaaccct
     2581 ttccggcggc gaagcccagc gcatccgcct cgccagccaa atcggcagcg gcctgaccgg
     2641 cgtgatgtac gttttggacg aaccctccat cggcctgcac cagcgcgaca acgaccgcct
     2701 gcttgccacc ctcaaacgcc tgcgcgattt gggcaacagc gtcatcgtgg tcgaacacga
     2761 cgaagacgcc atccgcgaag ccgatttcgt cgtcgatatg ggcccgggcg caggcgaaca
     2821 cggcggcaac gtactgattg ccgacacgcc cgaaaacgtc gccaaatgcg agcaatccat
     2881 taccggacaa tacctcagca gtaaaaaatc cattgccgtg ccgtctgaac gcacgcccgt
     2941 caatcccgac cggatgctcg tcctcaaagg tgcgcgcggc aacaacctca aaaacgttac
     3001 cctcgaactg ccgctcggtt tgattacctg catcaccggc gtatccggca gcggcaaatc
     3061 caccctgatt aacgacaccc tcgccaaaat caccgcccgc gaactcaacc gcgcccaaga
     3121 agaacccgcc ccatacgacg acatccgcgg tctcgaacat ctcgacaaag tcatcaacgt
     3181 cgaccaatcc cccatcggcc gcaccccgcg ctccaacccc gccacctaca ccggcctgtt
     3241 cacccccatc cgcgaactct tcgccggcat ccccctctcg cgcgaacgcg gctacaacgt
     3301 cggccgcttt tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag gcgacggcgt
     3361 gattaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag tctgccacgg
     3421 caaacgctac aaccgcgaaa cgctcgaaat ccaatacaaa ggcaaaaaca tcagccaagt
     3481 cctcgacatg accgtcgaag aagcccgcga atttttcgac gccgtcccca ccgtatcgcg
     3541 caaactgcaa accctgatgg acgtaggtct cggctacatc cgcctcggcc aatccgccac
     3601 caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ttggaactct ccaaacgcga
     3661 caccggcaga acactctaca tcctcgacga acccaccacc ggcctacact tcgccgacat
     3721 cgccctgctg ctggaagtca taggtcgtct gaaaggcaaa ggcaactcga ttgtgattat
     3781 cgagcataat ctagacgtga ttaaaaccgc cgattggatt gtggacttag ggccggaagg
     3841 cggcgatggc gggggcagga ttattgcttg tggtagccct gagcaggtgg cgaaagttaa
     3901 ggggagttat actgggaagt atttgaaggt ggttttgagg taagtttaat tcaaaattaa
     3961 ataggagatt attaagatga aaccgttaaa aacattagaa tttggatttg tggatgctgc
     4021 aaactacaga agaagagaaa ataaagattt atttaaccga atatttgtaa aaggagaata
     4081 tttggatgaa ttatgtgaac caaatatttc gtttttaatc ggagaaaagg gaactggaaa
     4141 gacagcatat gctgtttatt taactaataa cttctataaa aacatacatg ccactactaa
     4201 gtttgttcgt gaaaccgatt attcaaaatt tattcagcta aagaaagcaa gacacttaac
     4261 tgtttcagat tttacaagta tttggaaagt cattttatat ctgttgatat caaatcaaat
     4321 caaatgtaaa gaaaacggaa tattatcttc aatatttaat aaatttaaag ccttagatga
     4381 ggctataaat gaatattatt atggcgcttt tgatccggaa attgtacaag caataacttt
     4441 aatagaaaat tcaaaagaag ctgcggaaat gatttttgga aaatttgtta aactaggtga
     4501 agaggaatcc caacaaataa cttttacaga aagtaaattc caagcaaatt taggttttat
     4561 tgaaagaaaa tttaaagatg ctttatctca gttaaagcta aaagataatc atattttgtt
     4621 tattgatggg atagatatta gaccatcaca gattccattt gatgaatatc atgagtgtgt
     4681 aaaaggtctt gctaacgcca tatggatgtt aaataatgat atcttccctt ccattaaaga
     4741 tagtaaggga aggatgagag ttgtgttatt gattagacct gatatctttg attcattagg
     4801 tttacaaaat caaaatacca aacttcaaga taattcagta tttttagact ggaggacgga
     4861 ttataaatct tatagaagtt caaagatttt tggcgttttt gatcatcttt tgagaaccca
     4921 gcaagaaaaa caagatagtt tagaaaaagg caactcatgg gattattatt ttccatggaa
     4981 tgctcctaat ttacatgatg agtataaaaa tttaacttca tttattagct tcctaagaaa
     5041 atcgtattat cgacctcgcg atattcttca gatgcttact ttgctacaaa aaaataagaa
     5101 aagtaaggaa gattatgtcg tagcagaaga ttttgataat acttcttttc aaagagaata
     5161 ctcgatatat ttacttggtg aaatcaaaga tcatcttttg ttttattata gtcaaagtga
     5221 ttatcaaaat ttcctgaaat tttttgaatt tttaaacggg aaagatagat ttaaatatag
     5281 tgatttttta aaagcatttg aacgtttgaa aaagcactta caaacaacat cagtggaaat
     5341 acctaaattt atgagtactg ctaatgagtt tttgcaattt ttatttgact tgaatgttat
     5401 tgcttattta gataacccag aagatgaaac gaaaccatat atccattggt gctttaaaga
     5461 tagaaattat gcaaatattt ctcctaaaat aaaaactgaa actgaatatt taatattttc
     5521 aggattatca aaagcccttg atgttggtac tccatttaag aacaaacagt aacttgtatc
     5581 ccaaccaacc gtgtgcgcta cgcacacggt tggtagctga cgtgttgcag tttcaacaga
     5641 gaaagccgta gcctgcatac ccaaccgacc gcgtgcgtgg ctgtgccaca cgccctacct
     5701 gtgaattaca cggaacttca aagccaatac gagagctttg caaaattcct ttccctcccg
     5761 acagccgaaa cccaaacaca ggttttcggc tgttttcgtt tcaaatatcc actaatttta
     5821 ccccaatacc cccttaatcc tcccaggata cccgataatc aggcatccgg gccgcctttt
     5881 aggcgcaaac aggcacactt agcccgagac ctttgcaaaa ttccccaaaa tcccctaaat
     5941 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtctctga ctgccccaaa
     6001 agggaaaaaa taatgtgggt atgttgaggc tgcctctttt tgaaatgaca aacaaaatgc
     6061 cgtctgaaac ccgatttccg gtttcagacg gcatttgatg tttaatcagt tgtcaaacct
     6121 gattatgcca aaccttttgc cttcaacact tccagcatcg tcgtgcccaa ctcggcaggg
     6181 ctgcgagtat aggcgatacc ggctttttcg aaagcggcga atttttcttc ggcagtacct
     6241 ttaccgccgg aaataatcgc accggcgtgg cccatacgtt tgcctttagg agcggtaaca
     6301 ccagcaatgt aacctacaac aggtttggtt acgttggatt ggatgtattc ggctgcttct
     6361 tcttccgcag taccgccgat ttcaccaatc atgatgatgg cgtcggtatc tgggtcttct
     6421 tggaagagtt tcagtgcgtc gatttggttc ataccaggaa tcgggtcgcc gccgataccg
     6481 atacaggttg attgacccaa gcccagtttg gtggtttgtg ccacggcttc gtaagtcaat
     6541 gtaccggaac gggaaataat accgatgcgg cccggagtgt ggatgtggcc cggcataatg
     6601 ccgattttgc actcgcccgg agtaatcacg cccgggcagt tagggccgac caagcgggta
     6661 ccgttgccgt tggtttccaa gtaacgtttg gctttgagca tgtccaaagt aggtacgcct
     6721 tcggtaatca caacgaccaa gcctacgcct gaatcaacgg cttcaacgat agaatccaac
     6781 acaaacggag cgggaacgta aattacggat gcatccgcgc cggtttcttt aacagcttct
     6841 ttcatggtgt tgaatacagg caggttcagg tgggtttgac cgcctttgcc cggggtaacg
     6901 ccgccgacaa ctttagtgcc gtaagccaaa gcttgttcgg agtggaaagt accgttttta
     6961 ccggtgaaac cttgaaccaa tactttggtg tctttattaa tcaatacgct cattcttttc
     7021 tccttaggcg tttacggctg caacaatttt ttcggctgcg tcattcaggc cgtctgcaga
     7081 agtcagtttc aggcctgatt cgttcaggat tttcgcgccg agttcggcgt tgttaccttc
     7141 caaacgaaca acgacaggaa cgtcgacgtt gatttcttta acggctgcca cgatggcttc
     7201 cgcaatcatg tcgcaacgta cgataccgcc gaagatgttg atcaatacgc ctttaacgga
     7261 tttgtcttcc aaaatcagtt tgaacgcttc aaccacgcgg tctttggttg cgccgccgcc
     7321 aacgtccaag aagttggcag gttgaccgcc tttgagtttg atgatgtcca tggtggccat
     7381 cgccaaaccg gcaccgttaa ccatacagcc gatgttgcct tccagggcaa catagttcag
     7441 gtcgaattca gaagctttca gttcacgttc gttttcttga gatttgtcgc gcaattcagc
     7501 gatttttggc aggcggtaga gcgcgttgct gtcgatgccg attttgccgt ccacgcaagc
     7561 cagtgcgccg ttttcgcgaa ctgccagcgg gttgacttca aacagggcga agtcgttttc
     7621 gacaaacgct ttgtacgcgc cggtcatcag tttgacgaac tcgttgattt gtttgtcttt
     7681 caagcccagt tggaaggcaa cttcgcgagc ttggcaaggt tgcaggccga ccagcggatc
     7741 aacggttact ttgaagattt tttctggagt ttcggcagca actttttcga tttctacgcc
     7801 accttcggta gaagccatga atgtaacgcg gcgggtagaa cggtcaacca ctgcgcccaa
     7861 gtacagctcg gtttgaaccg gatacatgtc ttcgcaaacc aaaacgctgt tgacaggttg
     7921 gccgttggca tcagtttggt aagttaccaa gttggtgcca atcaggcttt cagccacttc
     7981 tttagcttct tcgcggcttt taacgacttt tacgccgccc gctttaccgc ggccgccggc
     8041 gtgtacttgt gctttgacaa cagcgaattt gccgcccaat ttgtcgtaag ctgcagcggc
     8101 ttcttcgccg ttgtgtgcca aaataccgcc ttgtacgggc aaaccgtagc tagccagcag
     8161 ttctttagcc tgatactcgt gtaaattcat ggatttctcc tttagattag tggaatgccc
     8221 cgagtttgcc tccaaatggc aagcccgtgc gtttcagact acctttcggc ggtcggaaaa
     8281 tcgtcgtttc acttgctgaa aggcaaccac atccgcgcca gcagatacat ggctgcaccg
     8341 cctcccccga gaataaaaaa gacgatgcct tttttacgcg aattcggaca gagcagataa
     8401 acggcaaggc tgaaactgat aaacaacaat gccacgcgcc aaaccaattc tttatctttg
     8461 aaacggttga gcgtataagt ttcggtcatc atcacataca tctgccacaa tgcggccata
     8521 cacatgctga tgatgcccgc cggtataaag aaaataccga ctatttcttt gttcataatg
     8581 aatgtaaatg ctgtttgttt gtctgacgga attataaatc agaaaacgaa gctctcggaa
     8641 cattcaattt gtcggaggcc ttaatgtcta tcaaccgtgc aaagcgcgtt tgtcggccgc
     8701 caatgcagct tcgtgaacca cttcggacaa ggttgggtgg gcatggataa tgcgggcgat
     8761 gtcttcgctg ctggcgaaga attcgagcgc agtcacgcct tcggtaacca attcgctgac
     8821 aaccggacca atcatgtgta cgcccaagat gcggtcggtt ttggcgtctg ccaacacttt
     8881 aaccgtacct ttggctttgc ccattgccaa tgcgcgacca ttcgcaccaa aacctgaagt
     8941 accttttttg tactccacgc cttcggcttt aagctgttct tcggttttac ccacccaagc
     9001 gatttcagga tcggtgtaaa taacaaacgg tacgttgttg aaatcgatat gcggtttttg
     9061 accggcaatg cgctcggcaa cggcaacgcc ttcgtcgctg gctttgtgtg ccaacatcgg
     9121 gccgcgaacc acgtcgccga ttgcccatac attaggcagg ttggtgcggc attcgccatc
     9181 tactttgata aagccgcgct catctttttc caagccgacg gcttcagcgt tcaggccttt
     9241 ggtgtttgga atacggccga tggcaacgat cagtttgtcg aatacttcgg ttttggcttc
     9301 gccggcagca gtttcgtaag caacggaaac acctttgcct tcagacttga tgtcgccgat
     9361 tttcacgccc aattcgatgc tcagaccttg ctctttggtg aagtatttga aggcttcttt
     9421 ggcgatttgt tggtcggcgg cagccaggaa ggtcggcgcg gcttcaagaa tggtaacttc
     9481 cgcacccacg cggttccata cggaacccat ttccaaacca atcacaccgg aaccgatcac
     9541 gccgagtttg gcaggtactt cggtcaggtt caatgcgcct tcgttgtcca atacgttcac
     9601 attgtcgata gcgacttgtg gcagcggacg cggtacggaa ccggtggcta cgatgacgtg
     9661 tttggcttcg ataacggttt tctcaccttt gttatcgact tcgatttggt aagcatcgct
     9721 atttttgccg gcaaaggaag ccgtaccgaa caggctggtt actttgtttt tctggaacag
     9781 gaatttcacg ccgccggtca gtttggttac gatggcatct ttgcgctcaa tcattttggc
     9841 cacgtcgaat tttacgtcgc cgacagtgat accgtgttcg gcaaactcgt gttgcgctgc
     9901 gtggaaatgt tcgctggatt gcaacagggc tttagaaggg atacagccta cgttcaagca
     9961 agtaccgccc aatgcagggg cattgcctgc tttgttaacg cctgcatcga cacaggcagt
    10021 tttgaaaccc agttgtgcgg cacggatggc ggcaacgtat ccgcccgggc ctgcaccaat
    10081 cactactaca tcatattgag acatcgtgtc atcctttgct tttagcagta tctcaaaata
    10141 aaaacgatac ttttcgtttc ttttttattt tgaatgactg cgttttgatg gttttaagaa
    10201 aaagccgtct gaaagtccat atctgccgat tcattcgaat caaatgtttc agacggcctc
    10261 aatcgttcgt tgagcctatt gtagcacgat actacattac gctacatcta aatatagtgg
    10321 attaaattta aatcaggaca aggcgaggca acgccgtact ggtttgaatt taatccacta
    10381 taaaaattca gacggccttt tcattaacaa ataaaaggcc gtctgaaacg attacagatc
    10441 caacaacagg cgggctgggt cttccaacgc gtctttaatg gctaccaagg tcaatacagc
    10501 ttcgcggccg tcaatgatac ggtggtcgta agacagagcc agatacatca ttggacggac
    10561 aacaacttgg ccgttttcaa ccacagcgcg ctctttagtg gcgtgcatac ccaaaatcgc
    10621 agattgaggc ggattgatga tcggggtgga catcatagaa ccgaaagtac cgccgttggt
    10681 aatactgaat gtaccgccgg tcagatcttc gatagcgatt ttgccgtctt tggctttttt
    10741 cgcgtaatca acaattgctt gttcgatgtc ggcaatgctc atttggtcgg catcgcgcag
    10801 gattggtaca accaaaccgc gtgggctgcc gatggcgata ccgatatcga agtagccgtg
    10861 atacacgatg tctttgccgt caacagaagc attcacaacc ggatattttt tcagggcggc
    10921 aaccgcggct ttaacgaaga aggacataaa gcccagtttt acgccgtgtt ctttctcgaa
    10981 tttttctttg tacttcgcac gcaagtccat gattggcttc atgttgactt cgttgaatgt
    11041 agtcagaatg gcgttttctt gttgagaagc caagagacgt tctgcaacac gggcacgcag
    11101 gcggctcatc ggtacgcgtt cttcgggacg tgcgccggca ggaagtgtaa cagcaggagc
    11161 agcggctgcg gcaggtttgg cagcggcatt ttgtacatct tctttcaata cgcgaccgtc
    11221 acggccggaa ccttgcaatg cgttcacgtc aacaccggtt tcggcagcca gtttggcggc
    11281 ggcaggcata gcggcgttgt tttgtgcagc agcaggagcg gcggccgggg cagcttcggc
    11341 aggagcggcg gcaggtgctt cagcagcggc agtagcagcg gtatcgatac gtgccaaaac
    11401 ttggtcggca acaacggttt caccgtcttg cgctacgatt tcaaccaata cgccggcttg
    11461 tggagaaggt acttccaaaa ccactttgtc cgtttcgata tcgatcagga tttcgtcacg
    11521 ggcaactgct tcgccaactt ttttcttcca ttccaagagc gtgccttcag atacgctttc
    11581 agacagcata ggtactttta catcaataat cattttgtgt ctccaatggc cctttcagac
    11641 ggcctgttgt gtgtttttca ttctgcatat gggtgttccg cctgagcagc ctcagacgga
    11701 atgctacttg gcttacaatg ccaaagcgtc ttcaaccaat tgtttcaatt gagcaatgtg
    11761 tttgcttgag tagcccactg caggcgatgc gctgcttgga cgaccggcat aagacagttt
    11821 ttgctcttcg ctgataacgt cttcgatgcg gtggcggatt tggtagaacg cgccttggtt
    11881 tttcggctct tcttgtgccc aaaccacaga ttttgcgttc ggatatttcg ccagttcggc
    11941 tttaacctcg tcgtatggga acggatacag ctgctcaacg cggacaatag cgacatcatc
    12001 ttccagttta cgttcggctc gaccggcttc caagtcatag taaacctgac cggcacacaa
    12061 taccacgcgt ttcacgctgt cgttgcttgc gcgttcggcg gtatcgccga taaccggacg
    12121 gaaagtcgaa ccttcggtga agttttccag cgggctcatt gcccctttga atcgcaacag
    12181 gcgtttggac atgaaaatca ctagcggttt gcggtatgaa cctaagactt gacgttgcaa
    12241 gaggtggaac atttgcgacg cttcagacgg catgatgact tgcatattgt tctcagaaca
    12301 cagttgcaac caacgttcta cgcgtgcaga agagtgctcg ggaccttgac cgtcgtagcc
    12361 gtgcggaagg atggtggtca gaccgcacaa acgaccccat ttggtttcgc ctgaagacag
    12421 gaattggtca atagtcactt gcgcgccgtt ggcgaagtca ccaaattgag cttcccaaat
    12481 ggtcagcttg tcaggcgcgg agcaggcaaa gccgtactcg aacgccatca cggcttcttc
    12541 gttcaaaatg gagtcgataa ccaagaactc gcccatacct tcgcccatat ggcgcagagg
    12601 aacataagta ccgtcatccc atttttcacg tttttgatcg tgcaatactg catgacggtg
    12661 tgagaatgta ccgcgcccgg agtcctcacc ggagatacgc acgccatgac ctttggttac
    12721 aaggcttgcg tatgccaagg tttcggccat accccagtcg atggcttgtt tgccggatgc
    12781 catggcttta cgcgcttcaa tcacacgttt tgcagtcgga tgcagggcaa agccttccgg
    12841 tacggcggta aatttctcgg taagacgctc aatatccgcg gcaggcaaac cggtttcgat
    12901 gtgttcgcgc caatctttac cttggtattt gctccagtca atttgtgtgc gttggaagtt
    12961 gctcaacgtt gtttgctcga cgtgttcgcc tttatccaaa gcatcacggt aggcttggat
    13021 gtaaccgtca gcctcggctt gggttaccac gccttcggca atcagttgct cggtgtacaa
    13081 agcacgcgca cccgggtgtt gcgatacttt tttgtacatc atcggttggg tcaaggtcgg
    13141 atcgtcgccc tcgttgtggc cccatttacg gtagcagaca acgtcgatca cgatgtcttt
    13201 atggaatttt ttgcggtaat ccaaagcggc ttggatggca aagcaaacac gctcaggatc
    13261 atcgccgttc acatggataa ccggagcgga aaccattttt gcgatatcgg tacagtgtac
    13321 ggttgaacgg gtatcgcgga tatcggaagt ggtaaagccg atttgattgt tgatgacgat
    13381 atgaaccgta ccgccggtgg tataaccgcg cgttttagac agattgaatg tcgcttggtt
    13441 gacacccaaa ccgataaatg cggagtcgcc gtgaatcaat acgggcaaga ctttatcgcg
    13501 gccgttttcg cccaaacgtt tctgcttggc gcgtgcagaa ccttcgacta ccgggttaac
    13561 gatttccaag tgtgacgggt tgaacgccaa agaaacgtgc atcggaccat gtggagtggc
    13621 gatgtcggag ctgaagccca tgtggtattt cacgtcgccg ctaggcaatt tgatttcggc
    13681 acggccttca aactcagcga acaaatctcc gggtttttta cccaaaatgt tcaccaaaac
    13741 attcagacgg ccacggtgcg ccataccgat gatgacttct tccacgccgt ctttaccggc
    13801 gttttgaatc aggtagttca aaccggcaat cgcgctttcg ccgccttcga caccgaaacg
    13861 tttctgaccg acatatttgg tatgcagata acgttccaaa gtctcggcag cagtcatctc
    13921 tttcaagata cggcgttttt gatcggcatt gtaatgcggt gtggacaata cgctttcaaa
    13981 ataattgcgt acccagcggc gctcttcggt attgggaata tagatatatt ccaatgcgat
    14041 gtggccgcag taggtttgtt tgaggttgct gatgatttgg gacaaaggca gtttgccgcg
    14101 attggcaaaa tcgccctcgc ccatattgaa ttgaagcgcc atatcggcat ctgacagacc
    14161 gtggaatttc ggatcgaggg cttcaatatc gcgcgggggg atacgtttga gcggatcaag
    14221 ttgggctgca cccacgcctt ggatacgata ggcggaaatc agccgtaaaa cgctgacttg
    14281 ctttttcagc attgcctcat ccgcaccgcc cgcaacggca gatgcaattt tctttttcgc
    14341 caaagtaaca aatgattcgc gaatcggtgt gtgtgcgaca tcgacagcaa ccgtccccgg
    14401 ctgtttgctc aaatcggtga aatactgctt ccatttttca tcaaccgcat cggggttttc
    14461 caaaaaagcc tcgtacaatt cctcaatgta aggtgcgttt gaaccgaaca ggtaagagaa
    14521 attgagtttt tcgtccatca tggcgtgcgc tcttttcttt aaaatataaa caaagggtag
    14581 aagtccgcgt cctgacggct aaactgatgc cgcctgaaaa gacattttat tctaccctat
    14641 tttaaaccgg attgcttgtc ttgatttcat ccgactacaa tcagacggat tcgggaaaca
    14701 atccggttct ctgatattgc ctgtttgaca atatattaat gtatgttacc tctcgcctgg
    14761 cggcacataa tcgcggcgtt ccgaaccagt ataaagctgg cgcgggcggc cgattttcag
    14821 cgaaggatcg ctaatcatct cgtgccagtg cgaaatccag cccacgctgc gcgacagggc
    14881 gaagatgacg gtaaacattt cggtcgggat gcccagcgcg gacaggacga tgccggaata
    14941 gaaatcgacg tttggataca gtttgcgttc gataaagaac gggtctttca gcgcaatctg
    15001 ttccaattcc atcgccagtt tgaatttcgg actgtcttcc aagcccaatt ccttcaaaac
    15061 ttcatagcag gtttcgcgca taatgctggc acgcggatcc atattgcggt acacgcggtg
    15121 accgaagccc atcagacggt atttgcgttg tttcacacct tccatgtatg cggcaacatt
    15181 agacacatcg ccgatttcgt ccaacatttt caacacggct tcgttcgcac cgccgtgcga
    15241 agcaccccac aggcaggcga taccggcagc aatacaggca aacgggttcg cacccgaaga
    15301 ccctgccaga cggacggttg aagttgaggc gttttgctcg tggtcggcat gcaaaataaa
    15361 gatgcggtcg agcgcgcgtg ccaaaacggg attgggtttg tagtcttcac acggcgtggc
    15421 gaacatcata tgaaggaagt tttcggaata agaaagatta ttcttcggat aattgaacgg
    15481 cagaccgttt gaatagcggt agcacattgc cgcaatggtc gggattttag aaatcaggcg
    15541 gtaaatcgcg attttgcggt gttcgggatt gctaatgtcc aagctgtctt ggtagaacgc
    15601 agacagtgcg ccgaccacgc cgaccatcat cgccatcgga tgcgcgtcgc ggcggaaccc
    15661 ccggaagaac caagtcagct gttcatgcac catcgtgtgg cggcggacgg tattgtcaaa
    15721 ttctgccttt tgctcgggag tcggcagttc gccgtaaatc aacaggtagc agacttccaa
    15781 ataatcggac ttttcggcca gctgctcgat ggggtatccg cgataataaa gcaagccttg
    15841 atcgccgtcg atgtaagtaa ttttagactc acagcttgcg gttgaaacaa atccggggtc
    15901 gaaggaaaac aaacctgtat tttttgtcag cccccgaatg tcaaccacat cgtgcccgat
    15961 gctggcttcc aataccggca gctccaaacc tgcctgaccc ggtacgttga gtttgattga
    16021 tttggacata ttattgctcc tttgcaagtt ataacccacg ttactgcatc cgcagcacgt
    16081 tctttgcctt cagacggctt ttgcattttg aaatctgcct gcaggcagat ttcagatgcc
    16141 tcatgcccgt ctgatttttt caagcatcgg gataaggtgc cctttgtccg tttccgaatg
    16201 cccgttaatc aaggcaagca attcttgatc ttgaaattca aggatttcgg aaaactcgga
    16261 cagctcttta tcgctcaaat gctcgaattc tttttccata aacctgccga agattaaatc
    16321 taattccaac aatccccggc gggtttgaaa acggattttc cgtttggcaa tatcgtcaaa
    16381 aaccatcatt tcttaaacgg cccgtttcaa cataatctct ttaatcttac cgatggctcg
    16441 ggtcggattc aagtgtttag gacatacgtc tacgcagttc ataatggtgt ggcaacggaa
    16501 caaacggtat gggtcgttca gattatccaa acgctcatta gtgatggtat cacggctgtc
    16561 cgcaatgaaa cggtaagcat tcagcaaacc ggacggacca acgaatttat cagggttcca
    16621 ccaaaatgac gggcaggcag tcgaacagca ggcgcacaaa atacactcgt acaaaccgtc
    16681 caactcttta cgctcttcct gagtttgcag acgctctttg tccgcatcaa tcggattatc
    16741 gttgacaaca taaggtttga cggaatggta ttgtttgaag aactgggtca tatccacaat
    16801 caggtcgcgg ataacaggca gacctggcag aggacggatt ttaactggct gtttcaagcc
    16861 acgcagatcg gtcaaacacg ccaagccgtt tttgccgttg atgttcatac cgtccgatcc
    16921 gcaaatgcct tcgcggcagg agcggcggaa agacaaggta tcgtcttgtg ctttcaggcg
    16981 taccaaagca tccaaaagtt tcacgtcggt cggttccaat tccaactcgt aacgctgcat
    17041 ataaggcttg gcatcaacat ccgggttgta acggtaaatt tcaaaactca ttttttccat
    17101 gagaagtgtt ccttttctca tcaactgccc aatgattttg tattataaat tcaagcagtt
    17161 caatgtgggt atggttagat ttcagacgag cttatgaggc gaaaaccgtc tgaaaacgca
    17221 tcaataaacg cgcttggccg gtttgatgta ttccacgctc aaaggcttgg tatgtaccgg
    17281 tttgtaagac aaagtattgg tatctgaatg gtacagcgta tgtttcatcc agttttcatc
    17341 atcgcgctca ggatggtcgt ctgaagcgtg cgcaccgcgt gattctttac gtgcttcggc
    17401 agacaccaaa gtcgctttcg ccacttcaat taggttatcc aattccaaag cctcgatacg
    17461 cgcggtattc cacactttgc tcttgtcttt gatttcggta cgtttcacac gctcggcaat
    17521 cgccatgact tctcgaacgc ctttgctcag aatctcatca gtacggaaca cgccggcgtg
    17581 caattgtacg gagcgttgca gttcgcggcg caatgcatca acgttttcac catcggtttg
    17641 gctgtccaaa cgctcgatac gttggcgggt caactcacca gcattagcag gcaaaggttt
    17701 ccagtcgctt tgctctttga tgaatttaat catgctgtcg ccggcagctt taccgaatac
    17761 caccaagtcc agcagggagt tcgtacccaa acggttcgca ccgtgtacgg aagcacaggc
    17821 gcactcacct gcggcataca ggccttttac aggcacttcg tattcgtctc cttgcggcac
    17881 aacgacttcg ccgtggtaat tggtcggaat gccgcccatc atatagtggg tagtcggcac
    17941 aacgggaatc gggtctttaa tcggatcgat accagcgaac tgaatggaaa tctcgcggat
    18001 gcccggcagt ttttccataa ttttttctgc gccgatatgg tcgattttca gtaagacatg
    18061 gtctttgttt ttaccgcaac cgcgaccttc gtagatttcc atcgccatcg cgcgggaaac
    18121 aacgtcgcga gaagccaagt cttttacggt cggcgcatag cgttccataa agcgttcgcc
    18181 gtcggcattc aacagaatac cgccctcgcc gcgtacgcct tcggtaatca acacgcccgc
    18241 acctgccacg ccggtcgggt ggaattgcca gaattccatg tcttccaacg ggatacctgc
    18301 acgcgcacaa atacccaaac catcgccggt attcatatag gcattggtag aagacgcata
    18361 aatacggccg ccgccgccgg tagcaaacat cacagcttta gcgtggaaaa tataaacttc
    18421 gccggtttcc atttccatgg cggttacgcc gacgacatcg ccgttttcat cacgaatcaa
    18481 atcttgtgcc gtccattcca caaagaattg cgtattggca cggacgtttt gttggtacaa
    18541 agtatgcagc atcgcatgac ctgtacggtc ggcaaccgca caggcgcgtt ctaccgcgcg
    18601 tttaccgtgt tcggcagtat ggccgccgaa aggacgctga taaattttac cgctttccac
    18661 acggtcaaaa ggcataccca tgtgttccaa ctcaattacg gcttcaggcg cggcgcggca
    18721 cataaactca atcgcatctt ggtcgcccaa ccagtcggaa cctttcacgg tatcgtacat
    18781 gtgccagtcc caacggtctt cctgcacatt acccagagag gcggaaatac cgccctgcgc
    18841 cgctacggta tgcgaacggg tcgggaacac tttagacaaa acggcacaat tcagaccgga
    18901 tttggataat tggagggctg cgcgtaaacc tgcaccacca ccgccgacaa tcacggcatc
    18961 aaacttgcga acaggaaaac ccatacttac ccccaaatta ctttaattga atacaccaag
    19021 cagccgacca gccagacgat ggtggcaacc tgcaaaaaca aacgcacgcc gaagggtttg
    19081 atatagtcca tccacaaatc gcggataccc acccaagcgt gcaagaatac ggcgatgaag
    19141 ctcacttggg taaatacttt tacccaagtt tgactaaaaa atgcctgcca tgccgaatat
    19201 tctttaggca gggaaaatag aaccactaaa agtgcaacgg tataaatcaa cataataacc
    19261 gcagtcgcac gttgcatcgc ccaatcgcgc aaaccgtaat gggcaccggt caatttacgt
    19321 tctaccataa caacgctccc aaaacgacag tcaaaaccaa tgcagaagca aatacggctt
    19381 tagcggtatt gcgcgcagta ttcagctcaa ggcctttgtg tgcatccaaa aataaaaagc
    19441 ggataccggc gagagaatgg tgcagataag cccacaatac accgattaaa accagcttga
    19501 ccaaaggatg ggaaacaatg gcacggtaag tttcaaatgc agactcttga ctcagggtac
    19561 cggacaggaa atacagcagg aaaggcagca taataaacag cccgaccccg ctgatgcggt
    19621 gaaggatgga aactatcccg ggtatcggca gacggatgtt cggcaaatcc agataaacag
    19681 gacgcggttt ggcagacata gtcaattcct ctgtagttat atatcgactc aaaaaccgta
    19741 aacccattgc tacactgggt tacataaacg ttaatttacc tgttttatcc cattttgaac
    19801 agtgtatttt aaaaacaaac tgatagattt tgacaaacac gcgtggaaac agcagattaa
    19861 aagaaagatt tttagttttg ccgccccctt cccgacacgg tatcagcatc cgaccccatc
    19921 cccaaaaaca aaatgccgtc tgaaaaaatt tcagacggca ttgttttgcc atatcggtca
    19981 ggctttcaat ttcgcatcgg catcgcgcaa cgcggtacgc agaccttcct cgataacggg
    20041 atggtagaac ggcatatcca gcatttgcgg aacggtcatc ttcatttgat gcgcccatgc
    20101 caacaagtgc gccaaatgtt cggcggcagg gcctacgatt tccgcgccga taaagcggcc
    20161 ggtggctttt tcggcataca ggcgcatatg gcctttgttc accagcatca cgcggctgcg
    20221 gccttggttt ttgaacgata cttcgccgat gacaaattcg tcggcttggt attgcgcggc
    20281 aacctgcgcg tatttcagac cgacaaagcc gatttgcgga ctggtaaaca ccacgccgat
    20341 ggtgctgcgc cgcaaaccgc cgccgatatt cgggtaacgg cctgcattgt cgccggcaat
    20401 cttgccttgg tcggcagctt cgtgcagcag gggcagttgg ttggacgcgt cgcccgcaat
    20461 aaagatatgc ggaatgctgg tctgcatggt cagcgggtcg gcaacgggta cgccgcgcgc
    20521 atctttttcg atattgatat tttccaaacc gatattgtca acgttcggac ggcggcccac
    20581 ggctgccagc atatattcgg caacaaatac gcctttttcg ccgtcctgct cccaatggac
    20641 ttctacattg ccgtctgcat cgagcttgac ctcggtttta gcatccaaat gcagtttcaa
    20701 ttcttcgccg aacacggctt tcgcctcgtc tgaaacgacg gggtcggaaa tgccgccgat
    20761 gattccgccc aaaccgaaaa tttcaacttt cacgcccaaa cggtgcaatg cctgacccag
    20821 ttccaaaccg ataacacccg gcccgaacac ggcaacgcgc ttaggcagcg tatcccatga
    20881 gaaaacgtca tcgttgataa tcaaacgatt gcccaaagac tgccattgcg gcaggatgac
    20941 gggacgcgaa ccggtagcaa tcacgaaact ttttgccgta atttgagtat gctcgtcgat
    21001 ttggacggta tgctcgtcga taaatttagc cgaacccatg atgcgcttgt cggcaggcca
    21061 ctcttccaca tcggcaacga caaagccgac aaaacggtca cgctcggatt tgacgcgctg
    21121 catgacctct tcgccgttga cgacgatgct gtctttgtcc aaatgcacgc cgaacgggtc
    21181 ggtatgcaat gcgtgatgac gcgcctctgc ggcggcaatc aagagtttgg aaggcataca
    21241 gcccacgcgc gcgcaggtcg tgccgaacac attgttttca atcaggtaaa cattatccga
    21301 atgtaaacgg gcattgcgaa acgcacccat accggcagta ccgccgccga ttacgacgac
    21361 atccgcttga atttttttca taatctttgt ccttccgttt tcgtctgaaa cattgccgac
    21421 acagaacata tcggtttcag acggcattta aatattttcc caatcaaaaa catcattcag
    21481 ataaataaaa acaaaatttc gattgagtgc aacaatatcc aaattctctt ataaatatag
    21541 agttattcgg agcggcagtg tagaaccgcc cgaataactc tatccggtat atcagacagt
    21601 ttaatcaaac cgccttaagc aaacagctat cagtttttag ccaagtaagc ttccaaatct
    21661 tcgctgccgc cgatgtattt gccgccgatg aagacttgag gggcagtcat cttgccggta
    21721 atggcgcgaa cggaagtaac ggttgcatct ttgcccaata cgatttcttc gtaagacaaa
    21781 cctttgtctt gcaaagcttg tttggcttta gcgcagaatt ggcaacctgg tttagtgaaa
    21841 attgccacag actcttgagc cttccaatcg ggagcaacga attgcagcat agtatctgca
    21901 tcggatactt tgaacggatc gcccagttct tcaggttcga tgaacatttt ttcaaccacg
    21961 ccgtcgttaa ccagcatgga gtaacgccaa gagcgtttac cgaagcccaa gtcttcttta
    22021 ccgaccagca tacccatacc ttcggtaaat tcgccgttgc cgtcaggaat catgtagatg
    22081 ttgtcggatt cttcttcggc agcccaagcg ttcattacga acgtatcgtt tacagatacg
    22141 cagtagattg cgtcaacgcc gttttctttg aacgcgccga acaattcgtt gtaacgcggc
    22201 aggtgtgaag aagaacaagt cggggtaaat gcaccgggca gggagaatac gactactttt
    22261 ttgcctttga acaaatcatc ggtagacaca tctttccaag tgtcgccgac gcgggtgcgg
    22321 aatactacgg aaggtacttt ttgaccggta cgatcttgca aagccatttt aagctcctta
    22381 atagattggt ttgagaaata attgtgaatc gctgggcatt ctaccccatg caaaatgatt
    22441 tgtataattt atagttcaaa taatataaat tatttaaaac tatcaaacac aactccgatt
    22501 tgcctgtttc ccacatataa agccgaatta gaaaaaaaca agctgcaatg aaaagatttt
    22561 ccggcaactg cgcccttgcg ccgcaaatca ggacaaaata ccgtctgaaa ggctttcaga
    22621 cggtatttat tcatacggtc tcggcttatt tcgccaattc ggcacgcagt tttcgggtta
    22681 cgttcatcat cacttggagt tgttccagag tttctttcca gccgcgtgtt ttcaggccgc
    22741 agtctgggtt aacccacaga cgttcaaacg gtacaacctc gatggctttg cgcaacaggt
    22801 gctcaacttc ggcttcagtc ggtacgcgcg ggctgtggat gtcgtaaacg cccgggccga
    22861 tgtcgttcgg gtatttgaat tcgccgaatg cggtcaacaa ttccatgtca gaacgtgaag
    22921 tttcgatggt aatcacgtcc gcgtccatgc tggcgatggc cggcaggatg tcgttgaatt
    22981 ccgagtaaca catatgggtg tggatttggg tactgtcttc gcaaccggtt gacgacaggc
    23041 ggaaggcttc gcttgcccaa gccaagtagg catcccaatc ggcacgtttc aacggcaggc
    23101 cttcgcggat ggccggttcg tcgatttgaa tcactttaat accggctttt tccaaatcca
    23161 atacttcgtc atttaaggcc aaggcgattt gtttgcacac ggtgctgcgc ggaatatcgt
    23221 tgcgcacaaa tgaccattgc agaatcgtta ccggaccggt caacatgcct ttcatcgggc
    23281 gtttggtcag ggtttgggcg taggttgacc aagccacagt catcgcgttc gggcggctaa
    23341 catcgccgta aatcaccggt ggtttcacgc agcgtgagcc atagctttgt acccagccgt
    23401 attgggtaaa ggcaaagcct tccagcaatt cgccgaaata ttccaccatg tcgttacgct
    23461 cggcttcgcc gtgtaccaac acatccaaat tgagtttttc ttgctcttca atcaccaagg
    23521 cgatttcttt tttcatcgcg gcttcgtaat cagcgtcaga caattcgcct tttttgaacg
    23581 cggcacgcgc ttggcggatt tcagtggttt gcgggaacga accgatattg gtggtcggca
    23641 gcaacggcag gttcagccat gcttgttgcg ctttaatgcg ctcggcaaac ggtgatttgc
    23701 gttggtcggc atctgccggt aaatcagcca agcgtttggc cacttcggcg cggtggattt
    23761 cggtgctgtt ggcgcgtgaa tcagcagcgg cttggctgtc ggccaattcg gcggcaacgg
    23821 cttcgcggcc ttggttcaat gcagttttca acacgcgcaa ttcttgcgtt ttctgcaagg
    23881 tgaaagccaa ccatgagaac aaggcaggtt tgttgacttt cagcttttct tctaccgaca
    23941 aatcgtatgg cgtgtgcagc agcgagcatg agctggaaat ccacaagcgg tcgcccaatt
    24001 tggctttcaa cggctcaagc gtggctaata cgttgtttaa attggcgcgc caaatattac
    24061 ggccatcaat cacaccggca gacaacactt tgccgtaatc agcaaatgcg tccaattgct
    24121 cgggcgcgcg caccaaatcg atgtgcaaac cggccacagg caaggctttc agacgacctg
    24181 cgtgtgcggc aaccgaaccg aaataagtgc tcaacaagat tttggcattc acttggctca
    24241 aagtggcgta aacgtcttgg tacgcatcca accacgcttg cggcaggtca accgccaatg
    24301 ctggctcatc gatttgaatc cattcggcac cggcagcggc caattcggtc aggatttcaa
    24361 cataaaccgg caataatttt ggcaacaggc tcaagcggtc aaattcgaca gcgccttttt
    24421 ctttaccaac ccacaggaag gtcaacggac caacaacggt cggtttggct ttcaggccca
    24481 aagcttgggc ttcttgcagc tgttgaacgt agtgtttggc gttcgctttg aattcggtat
    24541 cggcgtggaa ttcaggcacc aagtagtggt agttggtgtc gaaccatttg gtcatttcga
    24601 tagcgaattg gtctttattg ccgcgtgcca gttggaagaa ttgttccaaa gacaggtttt
    24661 ggctgtcgaa gccgaaacgg gcagggatgg caccggtagc gacttgcagg tcgaggatgt
    24721 ggtcgtagaa agtgaaatcg cctacagcaa cgtaatcagc gttggcagcg gcctggtgtt
    24781 tccagttttt ctcgcgcaag tctttagcaa cagccagcaa ttcttgctcg ctgatttctt
    24841 tgcgccagta tttttcttgt gcgaatttca attcgcggaa ggcgccgaca cgcgggaagc
    24901 ctgagaaatg taatgttgtc atgttgaact ctccagttgg attttgtaag gaccggttca
    24961 atccgaatcg gccggtattg taaatcagaa tggctcggat ggggcggtgt tttgcttttt
    25021 agacacctta gatgccgtct gaaactgttt gatacggctt taagggcgca cgcctaaaat
    25081 atggcaaatg gcgtaagtca gctcgctgcg gttaagcgta tagaagtgga aatctttcac
    25141 gccttcgcgg gacaggactt tgaccatatc gatggcgata cttgccgcca ccagattgcg
    25201 cgtaccttgg tcgtcgtcca aaccttcata catttgcgac agccagcttg ggattttgac
    25261 gttggttact tgagccattt taccgagctg cttgaagttg gtaacaggca aaataccggg
    25321 gacgatttcc acatcgatgc ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc
    25381 cacatcgaag aagaattggg tgatgacgtg gttcgcgccc gcatcgattt tgcgtttcaa
    25441 attaatcagg tcggcttgtg cggatttcgc ttcgggatgc acttcgggat atgctgctac
    25501 agaaatgtcg aagtcggcga cggagcgtaa tagcttaacc aagtcttcgg cataaaacgg
    25561 ttttttctcg taaccgggcg gctcgtcgcc gcgcagggcg acaatgcggc ggatgccgct
    25621 gtcccaataa tctttggcaa tttggcgcaa ttcgtcggga gaagcgtcga tgccggtcag
    25681 gtgcggcgcg gcttccaagc cggtttcttg cttaatgcgt ttgacgatgc cgtgcgtgcg
    25741 gtcgcgttcg ccggaatttg cgccgtaggt tacggatacg aacttgggat gcagggtttg
    25801 cagacggtgg atggaatccc acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa
    25861 ttcaaacgaa acgttgatgt cgcctttcaa atcggaaagg ctgttgttta acgcattgat
    25921 ttcttttgcg taattcatgg ctgcgtacct tttattatct tttatatgat atttcaggca
    25981 acataataaa attgcaccga atatgagtca atttcaaaat tttcaggttt atcatgaaaa
    26041 tttctaatat attacaaaaa tagttaaatt tccttgtaca agccttctaa atttatgata
    26101 aagtttgtta tgttataaca atccaactaa ggcgatatat gaaaccgaat attcatcctg
    26161 acaattatcg aaccgtcctt tttttcgaca gcagcgccaa tgaaggctgg ctgattcgtt
    26221 cttgcgccga aacacacgga aaaacgatgg tttggacaga cggcaaagaa tatccactgt
    26281 tttcgctgga tacttcttcc gcatcccatc ccgtctatac cggcaagcaa cgcaacgtca
    26341 acaccgaagg ccgtgccagc aaattcaacc aacgtttcca atccgtcatg tcatcgttta
    26401 gaaaggacaa ataatgcagg ttctgtcttc actcaaaacc gccaaacaac gccaccgcga
    26461 ctgtcagatt gtcagacgca aaggcaaagt ttacgtcatc tgcaaaagca atccgcgttt
    26521 taaagcgcgc caacgctgac cccctgccct tcctgcttcg gttgtaaatc ccctcctacc
    26581 gcaagccctt ttcatcaaat atgccgtctg aaacagaaaa atgcgtttca ggcggcattt
    26641 tttccgtcaa tacgaatccg gaatgtgttt gaaatacagc ataaacagat accaaaacag
    26701 catcgcggcg gctgcaccga gcagtaccca tgcgctgatt tcggcgactt cgcgcagggc
    26761 gcgtttttgc ctgcggctga aaaggcggtt gataatcaac gcggcaagtg cgaaaaagaa
    26821 aaaaatacgg aaaagtctgc ctatcatgtc gggaagcggt ggaggtcgtc tgaaaaagta
    26881 tgattcgtgt gcggcggatg agggtcaaac gcgtccggct gcttgtccga ccgtgtcaaa
    26941 aaatcatgcc gtttgcgata taataacgct tttcgccgca tttgttggaa caggatatga
    27001 gtcaaaatgc ctcggtgggc attgtaacgc cccaaaaaat tccgtttgaa atgccgctgg
    27061 ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat gattgaaacc tacggcgagc
    27121 tgaatgccga aaaaaataat gcggttttaa tctgtcatgc gctgtcaggc aaccatcatg
    27181 ttgcgggcag gcattcggcg gaggataaat atacgggctg gtgggacaat atggtaggac
    27241 ccggcaaacc gattgatacg gaacgttttt tcgtggtcgg tttgaacaat ctgggcggct
    27301 gtgacggcag cagcggacct ttgtcgatca atcctgaaac gggtagggaa tacggcgcgg
    27361 attttccggt ggttacggtg aaggactggg taaaatcaca agccgcgctt gccgactatc
    27421 tcggcatcgg gcaatgggcg gcggttgtcg gcggcagttt gggcggtatg caggctttgc
    27481 agtgggcgat ttcctatccc gaacgcgtgc gccatgcctt agtgattgcg tctgcgccga
    27541 aactgtccac gcaaaatatc gcgttcaacg atgtggcgcg tcaggcgatt ttgaccgacc
    27601 ccgatttcaa cgaaggacat taccgcagcc ataataccgt tcctgcacgg ggcttgcgga
    27661 ttgcccgcat gatggggcac attacctatc ttgccgaaga cggtttgggc aaaaaattcg
    27721 gacgcgattt gcgttccaac ggctatcaat acggttatgg cgttgaattt gaagtagaat
    27781 cctatctgcg ctatcagggc gacaaattcg tcgggcggtt tgatgccaac acctatttgc
    27841 tgatgaccaa agctttggac tatttcgatc cggcggcgga tttcggcaac agcctgaccc
    27901 gcgccgtgca ggatgtgcag gcaaaattct ttgtcgccag cttcagcacc gattggcgtt
    27961 tcgcgcccga acgttcgcac gaactggtca aggccctgat tgccgcccaa aaatccgtgc
    28021 agtatatcga agtcaaatcc gcacacgggc acgatgcctt tttaatggaa gacgaagcct
    28081 atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa ggaatgtcag caatgaacct
    28141 gcgcgatgat ttgcaactga tttacgaccg gatacccgaa ggcagccgcg tcttggactt
    28201 gggctgcggc gacggcgaac tgcttgccgc attggtcgaa cacaaaaaat gcagcggcta
    28261 cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg tcgcgcggcg tgaatgttat
    28321 ccaagccgat ttggaagaag gtttaaccgc attcaacgat caaagttttg atgtgattgt
    28381 gttgagccaa accatccaag cgatgcagaa taccgaaaaa atcctgcgct gcctgatgcg
    28441 cgttgccaaa caggcgattg tcagcttccc gaatttcggc tactggcgca accgcgtcca
    28501 aatcgcactc ggcggacata tgccggtttc cgaacgtatg ccctaccatt ggtacgacac
    28561 gcccaatatc cattggtgca cgctcaaaga ctttgatttg ttgtgcgcca aaaacaacat
    28621 ccgcgtcctc gaacgcgccg tgatgacggg caacagacag gtcaaacatt tccccaacct
    28681 tttgggcagc cttgcgtttt accgcgtcgg ctaagacagg ttgagaacct tgtcggcaat
    28741 aacaggttct tcggttctat taacaacagg ccgtctgaaa aatgaaacgg taggttttca
    28801 gacggcctct ttaatactgg aaacaataga atgaaaaaca aaacctcatc acttctctta
    28861 tggcttgccg caatgatgct gaccgcgtgt tccccgagca aagaagataa aacgaaagaa
    28921 aacggcgcat ccgccgcctc gtccacggca tccgccgctt cgtcttccgc gccccaaacc
    28981 gatttgcaac cggccgcatc cgcccctgat aacgtcaagc aggcagaaag cgtgccgccg
    29041 tcaaattgca ccgacctgca ccccgccacc ggcattgacg atctcatgca gcaaatcgcc
    29101 gaacacattg actcggactg tctgtttgcc ctttcccatc acgaactgga aacccgtttc
    29161 ggcttacccg gcggcggcta tgacaacata cagcggctgc tgtttcccga catccgccct
    29221 gaagatcccg actaccatca gaaaatcata ctggcaattg aagacttgcg ttacggaaag
    29281 cgcacgatca gccggcaggc acaagatgcc ttgatggaac aggaacgccg cctccgagaa
    29341 gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg gacaaggcga ggagccgaaa
    29401 cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt cgagccggca caacaacgga
    29461 cttggcggca atttccaata catcggccaa ttgcccggct atctgaaaat acacggagaa
    29521 atgcttgaaa accaatcact cttccggctg tccaaccgtg aacgcaatcc cgacaaaccg
    29581 tttttagaca tccattttga cgaaaatggc aaaatcacgc gtattgtcgt ttacgaaaaa
    29641 aacatctact tcaatccaaa cttggggcga agataaaaaa atgccgtctg aaacgttttc
    29701 agacggcatt tttcaatgca acaaatcaag cgcgtttgcg gaattcgccg gtgcgggtat
    29761 cgatttcgat tttgtcgccg ttttcgatgt aagacatcac ttggatttcg gtgcccccga
    29821 ccaggcgcgc ggttttcatc actttgcctg aagtatcgcc tttgacggca ggctcggtat
    29881 actcgacttc gcgtacgatg atggtgggca gttctacgga gatggggttg ccttcgtaga
    29941 aggtaacttc gcattggtct tccatgccgt caacgatgaa tttcaacgcg tcgccgatgt
    30001 tgtcggcttc gatttcgtat tggttgaatt cttcgtccat aaagacgtac atcggatcgg
    30061 caaagtagct gtacgtacag tttttgcggg acaggatgac cacgtcgaat ttgtcgtcgg
    30121 ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt tttcagtttc atgctgactt
    30181 tggcggaaga gcggccgcct ttgatgtatt cggttttttg aacgaccata ggatcgttgc
    30241 cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt tttcattgtg attttccaat
    30301 caaatcggtt gtcgtaaaac gcactattct agcgtatttt ttgatgcttt gaaacaaagg
    30361 cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata acggctccaa tcctccgcgc
    30421 cttgccgcca gccgttttga tgttgttgca ggatttgcca acattcgagg cgttgcgtgg
    30481 cggataaagc ccctccgccg ttgaggtcgt ccgaaagacg gcggtgtgcc gacacggttt
    30541 cgggcgtgta gaaaccgtgt gccttatccc aaaaggcgtg gagtttgtcg agatggacat
    30601 tctcgtcttg cgggtagatg tgccaaaaga agggtttgcc ggcaagctgg gcgcgcacga
    30661 aactgtcttc gccgcgtatc acggcgcagt cggcgaggtg cagcagtttg tcgaagtcct
    30721 gttgcggcac gaaagggatt ttgacgaggc ggacggatgc cgtctgaaaa acatcgccgt
    30781 cgttttgcag ggcatcttgc ggaataacgc cgctttgttt gaggctgtcg atgatttgcg
    30841 tccccgccag caacagtgtc atcgggctgc ctgcctgtcg ccacatttcc agccactttg
    30901 cccaaacatc gctccgatag ccgaaaagca gccattcggg ggcgtttttt tcgggaagca
    30961 tcagccgctg acgcagggct tcgctatcga aacggacggc ttcgcagtaa tcgcgttcgc
    31021 gtatcaaccc gccgcttttt tcgctgaaac ccataaacca aaaatatttt tgaacacctt
    31081 cctgcggcga aggcatcagg tgcagccttt cattgctttc ctccgcgctc aaatattccc
    31141 aattcagcca aagcggcttg tgtcggcgga taatgtgcag cacattttcg ggcaggtcgc
    31201 aggcaaaagt ttcgatgacg acatcgggaa caggcgcggt atcaatatct gccgcatcgg
    31261 aatgccaagt gcggacatga atatcctgat gaacgcaggg aacatcgggc aaatcagggc
    31321 aaagcgcacg caaggcggac acatcgtccg tccacaaatg cacctgccaa ccgagttcgc
    31381 ggtgcaaaac acgggcgagc cgccacgaaa cgccgatgtc gccgaaattg tcgatgacct
    31441 tgcaaaaaat ccaacagaca aaaggaggag tattcataga aataacggaa acgggctaaa
    31501 acgcgccgaa atagtggaac aaatacacaa tcagcaaaat aatcggaata aacagcaaca
    31561 tagtttgacg tttcaaatca aactcttgct tcaaatgatg cttgatggtg tcgaaaagga
    31621 cgaatttgaa caaaaacgtc gcttcgcagg caaggatgat gccgttaatc agatgctggg
    31681 gtttgtcttc gggcggattg gcggcgaaaa cggcaagcgt cgccaagggt atcaggcaga
    31741 cgacaagcca gcggacaagc aggtttcggg acattgcaag gctttcaaaa aagcaaaacg
    31801 ccattatcgc cccaagcgca cgccttgtct aacggatgcg ccggagatgc cgtctgaagc
    31861 cttgccgcag gaaaacaccg caccgccgaa cctttaaata cgcccgagac gggcgttccc
    31921 cgaaaaatcg gacgctgccg ccgatgcctg aggacaaggc ggcagcaggc acgggcatat
    31981 tgaaaacccg gcgcgtcggt ttg
//
LOCUS       DAWKOD010000005        30758 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000005 DAWKOD010000000
VERSION     DAWKOD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.005"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            142..1110
                     /locus_tag="WJK73_000156"
     CDS             142..1110
                     /locus_tag="WJK73_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7556700.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            1100..2074
                     /locus_tag="WJK73_000157"
     CDS             1100..2074
                     /locus_tag="WJK73_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ7556701.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2119..2745
                     /locus_tag="WJK73_000158"
     CDS             2119..2745
                     /locus_tag="WJK73_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556702.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2829..3587
                     /locus_tag="WJK73_000159"
     CDS             2829..3587
                     /locus_tag="WJK73_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7556703.1"
                     /translation="MITIRNVSYRIGTHPILDNISLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(3646..3984)
                     /locus_tag="WJK73_000160"
     CDS             complement(3646..3984)
                     /locus_tag="WJK73_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ7556704.1"
                     /translation="MKKIEAVIKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4079..4291
                     /locus_tag="WJK73_000161"
     CDS             4079..4291
                     /locus_tag="WJK73_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556705.1"
                     /translation="MPETVIPAQAGIPTSAFQQYLKVSVNPNFHIPDRAETTAFDPKT
                     YGSSCPLLCFLQLSAYPALGKNQKNR"
     gene            4449..8414
                     /gene="purL"
                     /locus_tag="WJK73_000162"
     CDS             4449..8414
                     /gene="purL"
                     /locus_tag="WJK73_000162"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ7556706.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLETFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPTVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAANIGDIGNIKLSANW
                     MTACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNERSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAAEAWPKFKRNLSEQFEARLSMVHVPK
                     SASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLN
                     PNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKAL
                     G"
     gene            8509..9261
                     /gene="gloB"
                     /locus_tag="WJK73_000163"
     CDS             8509..9261
                     /gene="gloB"
                     /locus_tag="WJK73_000163"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ7556707.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            9495..10949
                     /gene="mgtE"
                     /locus_tag="WJK73_000164"
     CDS             9495..10949
                     /gene="mgtE"
                     /locus_tag="WJK73_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ7556708.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(11035..11943)
                     /gene="hslO"
                     /locus_tag="WJK73_000165"
     CDS             complement(11035..11943)
                     /gene="hslO"
                     /locus_tag="WJK73_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ7556709.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            12188..12943
                     /locus_tag="WJK73_000166"
     CDS             12188..12943
                     /locus_tag="WJK73_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7556710.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            12962..13180
                     /locus_tag="WJK73_000167"
     CDS             12962..13180
                     /locus_tag="WJK73_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556711.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            13325..13672
                     /locus_tag="WJK73_000168"
     CDS             13325..13672
                     /locus_tag="WJK73_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ7556712.1"
                     /translation="MNIIRALLIILGCLATGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMSNLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            13669..14361
                     /locus_tag="WJK73_000169"
     CDS             13669..14361
                     /locus_tag="WJK73_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ7556713.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            14428..15648
                     /gene="argJ"
                     /locus_tag="WJK73_000170"
     CDS             14428..15648
                     /gene="argJ"
                     /locus_tag="WJK73_000170"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ7556714.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            15811..17220
                     /locus_tag="WJK73_000171"
     CDS             15811..17220
                     /locus_tag="WJK73_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7556715.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            17410..18801
                     /gene="hrpA"
                     /locus_tag="WJK73_000172"
     CDS             17410..18801
                     /gene="hrpA"
                     /locus_tag="WJK73_000172"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ7556716.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            18945..20588
                     /locus_tag="WJK73_000173"
     CDS             18945..20588
                     /locus_tag="WJK73_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7556717.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            20632..20814
                     /locus_tag="WJK73_000174"
     CDS             20632..20814
                     /locus_tag="WJK73_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556718.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            21026..22603
                     /gene="lpt3"
                     /locus_tag="WJK73_000175"
     CDS             21026..22603
                     /gene="lpt3"
                     /locus_tag="WJK73_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ7556719.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFHRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            22705..25968
                     /locus_tag="WJK73_000176"
     CDS             22705..25968
                     /locus_tag="WJK73_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ7556720.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGRGEGKTVASQTNFSATTANPLPNPLPQEGE
                     QSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDL
                     MQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEW
                     LVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVIIPQLAHFIAKSA
                     GDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHELQQQLGQAAAVTF
                     RDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTTEAA
                     EQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAF
                     IGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLL
                     RLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQM
                     WQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEDLN"
     gene            complement(25991..26413)
                     /locus_tag="WJK73_000177"
     CDS             complement(25991..26413)
                     /locus_tag="WJK73_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ7556721.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(26417..28063)
                     /locus_tag="WJK73_000178"
     CDS             complement(26417..28063)
                     /locus_tag="WJK73_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ7556722.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28077..29132)
                     /locus_tag="WJK73_000179"
     CDS             complement(28077..29132)
                     /locus_tag="WJK73_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7556723.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            29422..29634
                     /locus_tag="WJK73_000180"
     CDS             29422..29634
                     /locus_tag="WJK73_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556724.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            29944..30099
                     /locus_tag="WJK73_000181"
     CDS             29944..30099
                     /locus_tag="WJK73_000181"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ7556725.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(30189..30638)
                     /locus_tag="WJK73_000182"
     CDS             complement(30189..30638)
                     /locus_tag="WJK73_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7556726.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7644 a         8682 c         7296 g         7136 t
ORIGIN      
        1 gcgggaatgg cggcaagcgt ttttattgga acttgcccta tcctctgccc gatacggagg
       61 cttcaaacaa ggctttccgc tccgacggtt cggactgcct tgtttgaatc ttctacgcct
      121 taacgctttt cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc
      181 ctgctgctgg cggtgttgtt tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg
      241 tcggatgtgt tttcgctgtc ggacagccag caggttatgt tcatcagccg cctgccgcgc
      301 acgtttgcga ttgtgctgac gggcgcgtcg atggcggtgg cggggatgat tatgcagatt
      361 ctgatgcgta accgttttgt cgaaccttct atggcgggcg cgggtcagag tgcggctttg
      421 ggtttgcttc tgatgtccct gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt
      481 gccgccgttg ccgcgttaat cgggatgctg gtgtttatgc tgctgatccg ccgtctgccg
      541 ccgaccgcgc aactgatggt gccgctggtc gggattattt tcggcggcgt ggttgaggcg
      601 gtggcgacct ttatcgcgta tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag
      661 ggcgattttt caggcgtact gctggggcgg tacgagctgc tttggattac gggcggtttg
      721 gcggtgtttg cctatctgat tgccgaccgg ctgacgattt tggggctggg cgagacggtc
      781 agcgtgaatt tgggtttgaa ccggacggcg gtgttgtggt cggggctgat tattgtggct
      841 ttgattacgt cgctggttat cgttacggtc ggcaatatcc cgtttatcgg gctggtcgtg
      901 ccgaacatca tcagccgcct gatgggcgac aagctgcgac aaagcctgcc tgcggtggcc
      961 ttgctgggcg catctttggt gttgctgtgc gatattatcg gacgcgtgat tgtgtttccg
     1021 tttgaaattc cggtctctac ggtttttggt gtgttgggta cggctttgtt tttgtggctt
     1081 ttgttaagga aacctgctca tgccgtctga aaaaaatatc ggttttatgg caggaagcag
     1141 ccgtccgttg tgggtcgcct ttgcgctgtt gctggtttcc tgcgtcctgt ttatgacgct
     1201 caacgtcaaa ggcgattggg attttgtttt gcaactgcgg ctgaccaaac ttgccgcgct
     1261 gctgatggtc gcctatgcgg tcggcgtttc gacccagctt tttcaaacgc tgaccaacaa
     1321 tccgattctc acgccctcga ttttgggttt cgattcgctg tatgtgtttt tgcagacctt
     1381 gctggtgttt acgttcggcg gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg
     1441 ctttgaactt gccgttatga tgggcggctc gctgctgctg ttttacacgc tcatccgtca
     1501 gggcggacgc gatttgccgc gtatgatttt aatcggcgtg attttcggga ttttgttccg
     1561 cagcctgtcg tcgctgcttt cgcgcatgat cgaccccgaa gaatttacgg cggcgcaggc
     1621 gaatatgttt gccggattca ataccgtcca cagcgagctt ttgggcatag gcgcgctgat
     1681 tctgctcgtc agcgcggcgg tcgtttggcg cgaacgctac cgcttggacg tacacctttt
     1741 ggggcgcgac caagccgtca atttgggcat cagctacacg cgcaacacct tgtggatact
     1801 gctttggatt gccgcattgg tggcgacggc gaccgccgtg gtcggcccgg taagcttttt
     1861 cgggcttctc gccgcctcgc ttgccaacca cttttccccg tccgtgcgcc attccgtccg
     1921 cctgccgatg acggtttgtg tcggcggcat cctcctggtc ggcggacaga ccgtattcga
     1981 acacttcttg ggcatgaagg cggtattaag cgtggtggtc gaatttgcgg gcggactcgt
     2041 tttcctctat ctcgttttaa aacacaaaaa atgacggatg ccatctgaac ggcagcccgc
     2101 cccgaaagga caaaccatat gacgcaagaa cgtttacccg aatttttcga ccgcgccccg
     2161 acgctgaccg tacaagaccc gcttgccgca ttcctcggcg cggccgaaaa cggcatcctc
     2221 acttaccgct acgccgatgc cgtgcgcctg tgcggacatt cctgcccgac cgtcgcgggc
     2281 gcgtacctga tggttatcaa aggtctgaaa gccctttacg gagaagagct gcccgaacgc
     2341 ggcggcatcg aagccgccat gcagggagcg cgcgacgaag gcacggtcgg cgtaaccgcg
     2401 tccgtcgtcc aacttctcac cggcgcagcc cccgaaaccg gcttcggagg catcggaatg
     2461 cagggacgct tcgcccgccg caacctctta tcctttggtg caggcgaaat caacggcaca
     2521 ctgaccctgc gccgcaaaga caacggcaaa accgtcgccg tcagcctcaa cgccgccctg
     2581 caacccttcg cacccgaaat gcgcgaactt atgcccaaaa ccgtcggcgg cagcgcaagc
     2641 gcagaagaac tcgaacgctt cggacaactc tggcaggcac gcgttaaagc atttttgacc
     2701 gaatcggcgg acgacccgca gttcgtcatc gtccgcgaag tgtgagcgtt cagacggcat
     2761 tccgaatttc aaatgccgtc cgaacccccg ccaaacaaca acaaacctac gcccgacaag
     2821 catccgccat gattaccatc cgcaacgtca gctaccgcat cggcacgcac cccatcctcg
     2881 acaacatcag cctcgacatc cccgaaggcg gcattaccgc gctaatcggc ccgaacggcg
     2941 caggcaaatc caccctgttt tcctttatgg cgcggctgcg cccgcttgaa agcggcagca
     3001 tcgcctaccg cggcaaaaac cttgccggca cccccaccgc cgaactcgcc cgcacgctct
     3061 ccatcctcac ccaagaaaac agcatcatga gccgcatcac cgtgcgcgac ctgctgatgt
     3121 tcggccgtta cccctaccat caaggcagac cgactgccga atgccgccgt atcgttaacg
     3181 gtgcaatcga agaattccac ctgcaagact tcgccgaccg ctacctgacc gagctttccg
     3241 gcggacaacg ccaacgcgcc atgattgcca tggtgttctg ccagcgcacc gactacgttt
     3301 tgctggacga accgctgaac aacctcgaca tgtatcatgc ccgctccctc atgcaaatcc
     3361 tgcgccggct gaccgacgaa cacaagcgca ccaccgtcgt cgtattacac gacatcaacc
     3421 aggccgccgc ctacgccgac cacgtcgtcg ccatgaaaaa cggccaagtc gccatgcagg
     3481 gcaagcccga cgacattttc accgccgcaa acatcaaaac cctgttcgat atggatgtgt
     3541 ccgtcctcga ccaccaaagc aaaaaactca tcgtccacca tatctgaagc tcaaaaagaa
     3601 aatgccgtct gaaaaccttt cagacggcat ttccattcca aacgctcaaa ccgccgcgtc
     3661 cgcacgttcg cccgtgcgga tacggattgc ctcctcgacc ggcagcacaa aaatcttgcc
     3721 gtcgccgatt tttcccgaac gcgccacctc gacaatcacg tcaatcgcgc gttccacagc
     3781 atcatccgcc aacacaagct cgattttgac cttgggcagg aaatcgacgg cgtattccgc
     3841 accgcgatag atttccgtat gccccttctg cctgccgaac cctttgacct cgctgacggt
     3901 catgcccgta atgccgattt ccgtcaacgc ctcgcgcacg tcgtcgagtt tgaacggttt
     3961 gataacagct tcgatttttt tcatcacatt ctccattaat taacgtaaac aaacgacaaa
     4021 tatagcagaa accgccggca aacgataaac ggtactgttc gcccggcgat gcggcgattt
     4081 gccggaaacc gtcattcccg cgcaggcggg aatcccgacc tctgcatttc agcaatattt
     4141 aaaggtttct gtaaatccaa acttccatat tcccgaccgt gcggaaacga cggcatttga
     4201 tccaaaaaca tacggaagca gttgcccgct tctctgcttc ctgcaattat ccgcataccc
     4261 ggctttgggt aaaaatcaga aaaacagata aaacccttat tccttaatat cttcagacgg
     4321 cacctgtatt ttccccaacc gtatgccgtc tgaagattgt caacatttta agccaaaata
     4381 tccaagcaat acaaacctgt ccggcgtata atccctcttt ttcccaaccg catttgagag
     4441 ccgaatccat gtctgtcgtt ttgcccttgc gcggcgttac cgccctttcc gatttccgtg
     4501 tcgaaaaact cttgcaaaaa gccgccgcac tcggtctgcc cgaagtcaaa ttaagcagcg
     4561 aattttggta tttcgtcggc agcgagaaag cacttgatgc cgcgactgtc gaaaaactgc
     4621 aagccttgtt ggcggcgcaa agcgttgaac aaacgccaaa agcgcgcgag ggcttgcatt
     4681 tgtttttggt cacgccccgt ttgggtacga tttcgccgtg ggcttccaag gcgaccaata
     4741 tcgcggaaaa ctgcggtttg gcaggcattg agcgtatcga acgcggcatg gcggtgtggc
     4801 tggaaggtgc gcttaccgac aggcaaaaac aacaatgggc agccctgttg cacgaccgca
     4861 tgaccgaaag cgtgttgacc gacatcgatg cggcggcgca actgttccac cacatccaat
     4921 cggaaacttt ctccaccgtt gatgttttgg gcggcggtaa agaagctttg gtcaaagcca
     4981 ataccgaaat gggcttggca ctctccgccg acgaaatcga ttatctggtt gaaaactatc
     5041 aggctttgca gcgcaatccg tccgatgttg aattgatgat gttcgcgcag gcaaacagcg
     5101 aacactgccg ccacaaaatc ttcaacgccg atttcatcct caacggcgaa aaacaaccga
     5161 aatccctctt cggcatgata cgcgacacgc acaacgcgca tcccgaaggt acggtcgtcg
     5221 cttataaaga caattcgtcc gtaatcgaag gcgcgaaaat cgagcgtttc tatccggatg
     5281 cggcggaaaa ccaaggctac cgtttccacg aggaagacac gcatatcatc atgaaagtgg
     5341 aaacgcacaa ccacccgacc gccatcgcgc cgtttgcggg cgcggcgacg ggcgcgggcg
     5401 gcgaaatccg cgacgaaggt gcgacgggca aaggttcgcg tccgaaagcg ggcttgaccg
     5461 gctttaccgt gtccaacctc aacatccccg gcctcaaaca gccgtgggaa caagattacg
     5521 gcaagccgga acatatttcc tcgccgctgg acattatgat tgaaggcccc atcggcggcg
     5581 cggcgttcaa caacgaattc ggccgcccca acctcttggg ctacttccgc acctttgaag
     5641 aaaaatttga cggtcaggtt cgcggctacc acaaaccgat tatgattgcc ggcggtctgg
     5701 gcagcattca ggcgcagcag acgcataaag acgaaatccc cgaaggcgcg ttgctgatcc
     5761 aactgggcgg cccgggtatg cttatcggct tgggcggcgg cgcggcttct tcgatggata
     5821 ccggtacaaa cgacgcttct ttagacttca actccgtaca acgcggcaac cccgaaatcg
     5881 aacgccgtgc gcaggaagtg atcgaccgat gctggcagct tggcgataaa aacccgatta
     5941 tctccatcca cgatgtcggt gcaggcggct tgtccaacgc cttccccgaa ctggtcaacg
     6001 atgccggacg cggcgcggta ttcgagctgc gcgaagtgcc gttggaagaa cacggtttaa
     6061 cgcctttgca aatctggtgc aacgaatcgc aagagcgtta tgtattgtcg attttggaaa
     6121 aagatttgga gaccttccgc gccatctgcg aacgcgaacg ctgcccgttt gccgtagtcg
     6181 gcacggcgac cgacgacggc catttgaaag ttcgcgacga tttgttcgcc aacaaccccg
     6241 tcgatttgcc gctgaacgtc ttgctcggca aaccgcccaa aaccacgcgc accgacaaaa
     6301 cggttacgcc gtccaaaaaa ccgtttcacg ccggcgatat cgacatcacc gaagccgcct
     6361 accgcgtttt gcgcctgcct accgtagcag ctaaaaactt cctgattacc atcggcgacc
     6421 gcagcgtcgg cggcatgacc caccgcgacc aaatggtcgg caaataccaa acccccgtag
     6481 ccgactgcgc cgttaccatg atgggcttca atacctatcg cggcgaagca atgtctatgg
     6541 gcgaaaaacc gaccgttgcc ctgtttgacg cgcccgcctc gggcagaatg tgcgtcggcg
     6601 aagccatcac caacatcgcg gcagccaata tcggcgacat cggcaacatc aaactctccg
     6661 ccaactggat gacggcgtgc ggcaacgaag gcgaagacga aaaactctac cgcaccgtcg
     6721 aagcggtttc caaagcctgt caggcattgg atttgagcat tcccgtgggc aaagacagcc
     6781 tgtcgatgaa aaccgtgtgg caggacggcg aagagaaaaa atccgtcgtt tcgccgttga
     6841 gcctgattat ctccgcgttc gcgccggttc aagacgtacg caaaaccgtt acgcccgaat
     6901 tgaaaaacgt cgaggacagc gtattgctgt ttgtcgattt gggcttcggc aaagcgcgta
     6961 tgggcggctc tgcattcggt caggtataca acaacatgag cggcgacgcg cccgatttgg
     7021 acgacacagg ccgtctgaaa gcgttttaca acgtgattca acagcttgtc gccgaagaca
     7081 aactcttggc ataccacgac cgcagcgacg gcggcttgtt tgccgttttg gtagaaatgg
     7141 cgtttgcagg gcggtgcggc ttggatatag atttaaattt attgcttgca caaacattta
     7201 ttaccaacca taccgctctg tctcaatcat tgcggactga agaggtaaaa gcgttggctg
     7261 aatggcaaga aaccattgcc cgcacattat ttaatgaaga gttgggtgct gttatccaag
     7321 ttagaaaaca agatgttgcc gatattatca atttattcta tcaacaacag ctgcatcata
     7381 atgtctttga aatcggtacg ttaactgatg agaacacgtt aatcatccgc gacgggcaaa
     7441 cgcaccttat ttctgacaac ctaatcaaac tgcaacaaac ctggcaagaa accagccatc
     7501 aaatccaacg cctgcgcgac aaccctgcct gcgccgacag cgagttcgca ctgattggcg
     7561 acaacgaacg cagcgcattg tttgccaacc tgaaattcga cgtgaacgaa gacatcgccg
     7621 cgccgtttat caacagcggc gcgaaaccca aaatcgccat cctgcgcgaa cagggcgtga
     7681 acgggcaaat cgaaatggcc gccgcgttca cccgtgccgg attcgatgcc tacgacgtgc
     7741 atatgtccga cctgatggca ggccgcttcc gccttgccga cttcaaaatg ctggcggcgt
     7801 gcggcggctt cagctacggc gacgtactcg gcgcgggcga aggctgggcg aaatccatcc
     7861 tgttccaccc tgctctgcgc gaccagtttg ccgcattctt cgccgacccg gacacgctga
     7921 cattgggcgt gtgcaacggc tgccaaatgg tcagcaacct cgccgagatt atcccgggtg
     7981 cagcagaggc atggcctaaa ttcaaacgca acctgagcga acagtttgaa gcacgcctga
     8041 gtatggttca tgtccccaaa tccgcgtcgc tgattctgaa cgaaatgcaa ggctccagcc
     8101 tgcccgtcgt cgtcagccac ggcgaaggcc gcgccgactt cgcgcttcac ggcggcaata
     8161 tttccgccga tttgggcatt gcgctgcaat atgtggacgg tcaaaaccaa gttacccaaa
     8221 cctatccgct caaccccaac ggctcgccgc aaggcatcgc cggcattacc aacgccgacg
     8281 gccgcgtgac catcatgatg ccccaccccg aacgcgtgta ccgtgccgca caaatgagct
     8341 ggaaaccaga agactggacg gaattgtccg gctggtaccg cctctttgcc ggcgcacgta
     8401 aagccttggg ctaaccgccc tactcaaacc aatgccgtct gaagaatatt tcagacggcg
     8461 ttccggcata ccatccttta aacggtatcc gtccaccgag gaacactcat gaaaatcacc
     8521 cccgtcaaag ccctaaccga caactacatc tggatgatac agcacggcaa ccatgccgtc
     8581 tgcgtcgacc cttccgaacc ctcgcccgtc ttggaattcc tcgtccgcaa ccgcctcatg
     8641 cttgcccaaa catgggtaac gcacccccat cccgaccacg agggcggtgc ggcggcactc
     8701 tggcgcggct acatggaatc gcccgtttac ggcgaatccg acatcgaagc agcaacccac
     8761 accgtaaccg ccggcaccca attcaccttc ggcgacggac aggttaccgt ttgggcaaca
     8821 cccggccaca cagaccgcca caccagctac cttctcgaaa cttcagacgg catacacgtc
     8881 ttttgcggcg acaccctttt ttccgccggc tgcggacgcg tgtttaccgg cacaatcgaa
     8941 cagctttacg acagcttcca acgcttcaac cgcctgcctg aaaacaccct gttctacccg
     9001 gcacacgaat acaccgccgc caacctgcgt ttcgccgccc atatcgagcc ggacaacgcc
     9061 gacattcaga cggcattgaa agcggcagaa cgcacgccca ccctgcccgt taccctcgcc
     9121 cacgaacgcc gcgtcaaccc gttcttacgg acagaaatcc ccgccgtccg ccaacgtgcc
     9181 gaagccctgg tcggcaaaac gctgaacagc ggtttggaag tattcgccgc cctgcgcgaa
     9241 ctgaaaaacg cctactgctg acctgccctc cgaaaaatgc cgtctgaaac ccgcgtttca
     9301 gacggcattt gcgttaaaaa tagtaaaccg tttcaaaagg gagtagaata gtgccgtttc
     9361 caaccctgcg cctgtaccgt caggctttta ttatggacct tcccagttcg tttttactga
     9421 acaccccttc cgattccaaa cgacaataac catcccgccg gaatgcctcc ccgcgcacgg
     9481 cgggcggagc atttatgagc atcgaaccaa cccctccgaa ccttgaaaac gacggtatcg
     9541 aaaacgatgt agaacgcgtt tccgccgatt tcgaccgtgt ccactccctc tgcgaaatcc
     9601 tcgaacctgc ttttgaacaa atcgaaaacg gcacaccgct cgaagacgcg ccgctgcgcg
     9661 acaagctgac cgagctgacc gtcctcttgg gcgagctgca ccctgccgac gtggcggcgg
     9721 tgttggaatc gctgccgccg cgcgaacgca acgtcgtttg gcttctggtc aaacccgaag
     9781 acgacggcga agtattgctg gaagtatccg acgcggtgcg cgaaacactg atcgagtcga
     9841 tggataagga cgaattgttg gcggcagttg acgatttgga cgcggacgaa ttggcggaac
     9901 tggcagacga tttgccgcac caagtggttt acgaagcctt acagacgcgt gatgaggaag
     9961 aacgcgccca agtcaaggcg gcaatgtcct acgaagacaa ccaagtcggt gcgattatgg
    10021 acttcgagtt ggtcagcatc cgcgccgatg tcgcctgcga agtggtgctg cgttacctgc
    10081 gccgcttcga cagcctgccc gaccacacgg ataaaatctt cgtggttgac gaaaacgatg
    10141 tgttgcaggg cgtgctgccc atccgcaaac ttttggtcgc cgatcccgaa gacttggtgg
    10201 aaaacgtgat ggcgaaagat gtcgtgcgtt tccgcgccga agacgacgtg gaagaagcag
    10261 cgcaggcgtt tgaacgctat gacttggtta ccgcgcccgt cgtcgatgaa aacaaaaagc
    10321 tcatcggcag ggtgaccgtt gacgaaatgg tggacgtgat ccgcgaagaa tccgaagcag
    10381 atatgttgaa catggcgggt ttgcaagaag aggaagactt attcgccccc gtcttggatt
    10441 ccgtcaaaaa ccgctggatg tggctcgccg tcaacctctg caccgccttc cttgccagcc
    10501 gcgtcatcgg cgcgtttgaa ggcagcatcg aaaaaatcgt cgcactcgcc gcgctgatgc
    10561 ccatcgtcgc cggtatcggc ggcaactcgg gcaaccagac gattaccatg attgtccgcg
    10621 ccatggcgat ggggcaactg acggatatgc aggcggggcg tttgctgaaa aaagaagtcg
    10681 gcgtcgcctt ggtcaacggc atcatttggg gaacggtcat gggcgcagta tcttggctgc
    10741 tttacggcag cctcggcatc ggtttggtca tggttgccgc gatgacgctc aacctgctgc
    10801 tggcggcaac cgtcggcgta ttaattcccg tggtaatgga aaagttcgga cgcgatcccg
    10861 cgctgggcag ctcggtgctg attacggcgg taacggattc cggcggcttc ctgattttct
    10921 tgggtctcgc caccctgttc ctgctttaaa tgccgtctga acccgcgcaa aaatgccgtc
    10981 tgaagcggaa gctgcttcag acggcatttg actatttatc cttgttgcac aagattattg
    11041 gacggtatgc cggggcagcc ctttggcaac gccgaccaca tcctccccga acagcgcgtt
    11101 gacatcggtt tcgtcaaaca catatttgct gtggcagaaa tcgcaatcga cttcgatgct
    11161 gccttgttcc accaccacgc cgccgacttc ttccccgccc agcatcagca gcatatcgct
    11221 gactttgccg cgcgaacagg tgcatgaaaa ttcaaacgtt tccggctcga acacgcgcgg
    11281 cggcgtttcg tggaacaggc ggtacaaaac atgttgcgca tccaattctg ccagctcgtc
    11341 cgccgtcagt gtacgcgcca gcgtactgac gtgttcccat gcctcttcat ccaacaccgc
    11401 ttcaggcaga cgctgcacca gcagaccgcc cgccgcttcg tcgcttgcag acaggacgat
    11461 gtgcgtatcg atttgttcgg aacgcttcat atagttcgcc aacatttgcg cgataccgcc
    11521 gccttccaaa ggcactacgc cctgccaggg ttcgccgtct ttgggctgca gcgtcagcac
    11581 gaatacgccg ccctcgccca aaaggtcgcc gaggctttcg tcatcggcta tttctgcggt
    11641 ttcgtcccaa cgcgcggttg cacggacggt acggtcggaa gccgcttccg caaccagcat
    11701 tttcagccgc ccccgcccct gaacctgcac aatcagcgtg ccttcgtttt tgaggttgcc
    11761 cgacagcaac acacccgccg ccaacaactc acccaaagcg cggcggatgg cggcgggata
    11821 gtttttctgt tttacaatgt gctgccacac gttttccaga cggacgtgca gcccgcgcac
    11881 gggcatatcg tcgaagataa agcgggtacg cacatcggcg cggttgatgg cggtttgatt
    11941 catgattttc tctgactgat tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa
    12001 caagacggac ggcggatgcg cttcccaaat tatcaataaa ttatataaaa atcaacatat
    12061 taactcaatc taacaagccg ttttttgcca aacagccgtt tttttatata caatcaacaa
    12121 gatattttcg actgatacag cataacatcg cacggcggca cgatgcctcc tgcgcggaaa
    12181 caccgatatg gattcttttt tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc
    12241 cgtccgcccc gcccttgccg acgagttgac caacctgctc agcagccgcg agcagattct
    12301 cagacagttt gccgaagacg aacagcccgt tttacccatc aaccgagccc ccgcccggcg
    12361 ggcgggcaat gccgacgaac tcatcggcag cgcgatgggg cttaacgaac agcccgtttt
    12421 acccgtcaac cgagtccccg cccggcgggc gggcaatgcc gacgaactca tcggcaacgc
    12481 gatggggctt aacgaacagc ccgttttacc cgtcaaccga gcccccgccc ggcgggcggg
    12541 caatgccgac gaactcatcg gcaacgcgat gggacttttg ggtattgcct accgctacgg
    12601 cggcacatcg gtttctaccg gttttgactg cagcggcttc atgcagcaca tcttcaaacg
    12661 cgccatgggc atcaacctgc cgcgcacgtc ggcagaacag gcacggatgg gtacgccggt
    12721 tgcccgaagc gaattgcagc ccggagatat ggtgtttttc cgcacgctcg gcggcagccg
    12781 catttcccat gtcggacttt atatcggcaa caaccgcttc atccacgcgc cgcgcacggg
    12841 gaaaaatatc gaaatcacca gcctgagcca caaatattgg agcggcaaat acgcgttcgc
    12901 ccgccgggtc aagaaaaacg acccgtcccg ctttctgaac tgatttccca aggaatacgc
    12961 aatgagtatg cccgaaatgc ccaaatggta cgacgatgac ggacagatcg tgtcctgtac
    13021 cgaaaaggtc aaagtgatgt ccgaaaatat ggccgagctg tatcagacgg cacaagacgc
    13081 gtttgaagac gcgctgctga tgggttgcgg cgaacgtcag ttgcgcgatt acctgctcgc
    13141 gctgattgaa ggtttggaaa atccctaccg caaagtctga acacgccccg gttgctgcgg
    13201 cacggtttat ccgtgccgtt tttgcgtttg tgcgcggctt cggcttttca gacggcatat
    13261 ttgacgttat gattaaacag ttaacaagat ttatcacaac gccgtcaaaa gacagacaca
    13321 caacatgaac atcatccgcg cgctcctcat catcctcggc tgcctcgcca ccggcgaaac
    13381 cgccgttttc ctagcaggca tcaaactgcc cggcagcatc gtcggcatgg gcgtgctgtt
    13441 tgcgcttttg caggcgggtt gggtcaaaac gtcttggctg caacagctta ccgacgcgct
    13501 gatgtcgaac ctgacgctgt tcctcgtgcc gccctgcgtg gcggtcatca gctatttgga
    13561 tttgattgcc gacgattggt tttcgatact ggtttccgcc tccgccagca ctttgtgcgt
    13621 actgctggtt acgggcaaag tccaccggtg gatacggggt attatccgat gaacgaaatc
    13681 ctcaggcagc ccagcgttct gcttttcctc acgcttgccg tgtacgcgct tgcgattatc
    13741 gtgcgcacgc gcacgggcaa tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg
    13801 attgcctacc tgaaaatcct cggtatcgat tatgcggtgt accacaacgc cgcgcaattc
    13861 attgattttt ggctgaaacc cgccgtcgtc gtgcttgccg tgccgctcta ccaaaaccgc
    13921 cgtaaaatct tcaaccagtg gctgcccgtc atcgtttcac agcttgcggg cagcgttacg
    13981 ggcattgtta cagggatgta ttttgccaaa tggctgggcg cggaacgcga agtcgtcctc
    14041 tcgctcgcgt ccaaatctgt taccaacccc atcgctattg aaatcacccg ctccatcggc
    14101 ggcattcccg ccattaccgc cgccaccgtc atcattgccg gtctggtcgg acagattgcc
    14161 ggttacaaaa tgctgaagaa cacggtcgtc atgccctcgt ccgtgggtat gtcgctcggc
    14221 acggcttcgc acgcgatggg gattgccgcc tcgctcgaac gcagccgccg tatggcggca
    14281 tacgcggggc tggggctgac gttcaacggc gtactgaccg cgctgattgc gccgctgctc
    14341 atccccgttt tgggattttg aacccgtttc agacggcatt tcagcccatg ctgtctgaac
    14401 gccgacacac tcgcaaggag aaccgttatg gctgtcaacc tgaccgaaaa aaccgccgaa
    14461 caactgcccg acatcgacgg cattgccctc tacaccgccc aagcaggcgt gaagaagccc
    14521 gggcataccg acctgacact gattgccgta gccgccggca gcaccgtcgg tgcagtcttc
    14581 acgaccaacc gtttctgtgc cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa
    14641 gacggcgtgc gcgccctcgt catcaacacg ggcaacgcca acgcgggtac gggcgcacag
    14701 ggcagaatcg atgctttggc agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg
    14761 aaccaggtgc tgcccttctc caccggcgtg attctcgaac cgctgcccgc agacaaaatc
    14821 atcgccgccc tgcccaaaat gcagcctgcc ttctggaacg aagcggcacg cgccatcatg
    14881 accaccgaca ccgtgcccaa agccgcctcg cgcgaaggca aggtcggcga caaacacacc
    14941 gtccgcgcca cgggcatcgc caaaggctcg ggcatgattc atcccaatat ggcgaccatg
    15001 ctcggtttca tcgccaccga tgccaaagtt tcccaacccg tcctccaact gatgacgcag
    15061 gaaatcgccg acgaaacctt caacaccatc accgttgacg gcgacaccag caccaacgac
    15121 agcttcgtca tcatcgccac cggcaaaaac agccaaagcg aaatcgacaa catcgccgac
    15181 ccgcgttacg cccaactcaa agaattgttg tgcagcctcg cgctcgaact cgcccaagcc
    15241 atcgtccgcg acggcgaagg tgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa
    15301 acccgcgacg aagcccgcca agccgcctac gccgtggcac gttcgccgct ggtcaaaacc
    15361 gcctttttcg cctccgaccc caacctcggc aggctgctcg ccgccatcgg ttatgccggc
    15421 gttgccgacc tcgataccga cctcgtggaa atgtatctcg acgatatttt ggttgccgaa
    15481 cacggcggac gcgccgcaag ctacaccgaa gcacaagggc aggcggtaat gtcgaaggcc
    15541 gaaatcaccg tccgcatcaa gctgcatcgc ggacaagccg ccgccaccgt ctatacctgc
    15601 gacctgtcgc acggatacgt ttccatcaac gccgactacc gttcctgacc cgacacggct
    15661 tcagacggca tacatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
    15721 agtacaaata gtacggcaag acgaggcaac gctgtactgg tttttgttaa tccactataa
    15781 aatgccgtct gaaccgccgg acaacatacc atgacctcca cattcccccg ccgcctcgcc
    15841 cgcaaaatcc gccaaacccg ccgcctgtcg cgcaaaagca tcgcctttct gttccttttg
    15901 gcaggttcgg cactcgtcgc cctgaccgcg ctgttttttg cccatcttgc cgattttgcg
    15961 ctggaactga acgccaaact ggttcaacaa tacccgtggt tcgcgtgggt cgcgcttcct
    16021 ttgggtttac cgcttattgc gtggctcaca cgcaaattcg cccccttcac cgccggcagc
    16081 ggcatcccgc aggtcatcgc ctcactgtcg ctgccctacg gcgcacagaa aacgcggctg
    16141 atccgcctcg ggcagacgct gctgaagatt ccgctgacct ttttgggtat gctgttcggc
    16201 gcgtccatcg gacgcgaagg tccgtccgtg caggtcggcg cggcagtgat gggcgcgtgg
    16261 ggcgcgtggt gcaagaaaca cggcttggca ttcaaaggga tgcaggaaaa cgatttgatg
    16321 gcggcgggcg cggcgggcgg tttggcagcc gcgttcaacg cgccgctggc gggcgtgatt
    16381 ttcgccattg aggaactcgg gcgcggcatc atgttgcgct gggagaggca aattcttttg
    16441 ggcgtgctcg cctccggttt catacaggtc gccattcagg gcaacaaccc gtatttttcc
    16501 ggcttcaacg gcggcgtatt ggaacatatc tttctgtggg tcgcactgtc cggcctggtt
    16561 tgcggcgcgg cgggcgggct gttcggacgt ttgctctatc gcggtgcggc ggcgtttgca
    16621 ccgcgcaaga tacgcggctt catccgcaac cgtccgctgc tgctggcggc actgatgggg
    16681 ctgctgctcg ccctgctcgg cacgttctac caaggcaaaa cctacggcac cggctaccac
    16741 gaagccgccc aagccctgca cggcatctac gaagccccct tcggactcgc cgccgccaaa
    16801 tggctcgcca ccgtattcag ctattgggca ggcattccgg gcggcatttt cactccctcg
    16861 ctgaccatag gcgcggtttt gggcgagcat atcgccgcca tcgccgacat atcgcagggt
    16921 gcaaacatca tcgtcctcat ctgcatggcg gcatttctgg cgggcgcgac acaatccccg
    16981 atcacttccg ccgtcgtcgt catggaaatg acgggcggac aaagcctgct gttttggatg
    17041 ctaattgcct gcattttcgc ctcgcaggtt tcgcgccagt tttcgccgcg tccgttctac
    17101 cacgcatcgg gaatgcgctt ccgccagcgc gtgcttcaag aaaccgccgc ccaaaccggc
    17161 aatgcgcccg caagaccgca aacagcaaac agcaaaacgg gaatgccgtc tgaaaattaa
    17221 aacgcccccg atcaaacgcc ggcagccgcc ttgatttgaa taccgttccg ccgccgcttg
    17281 aaacttcagc aacaatgccg tctgaacgac agaatgcggt tttcagacgg catttcccca
    17341 tcccgatatt gcccgaaccc aaccaaagcg tttgctataa ttctattttt taccgcatac
    17401 gcaccaatca tgtttcccga tttctcccaa accctctcca aagaccgcca cttcctgcaa
    17461 tccgccttca aaaatcccaa caaatacggc ggcttgtcca aagtcgaaga aaaataccga
    17521 aaatcgcacg aaatcttttt gaagcgtttg gctgccttgc ccaaacccga attcgataac
    17581 accctgcccg ttcacgagaa gctcgacgaa atcaaaaaag ccattgccga gaatcaggta
    17641 acgattattt gcggcgaaac cggttcgggc aagaccactc agttgcccaa gatttgtctg
    17701 gaactcgggc gcggggcggc gggtttgatc gggcataccc agccgcgccg tttggcggcg
    17761 cgttccgtag cagagcggat tgccgaagag ctgaaatccg aaatcggcag cgcggtcggc
    17821 tataaagtgc gcttcaccga ccacacctcg cgcgatgcct gcgtcaagct gatgaccgac
    17881 ggcatcctgc tggcggaaac ccagaccgac cgttatctcg ccgcctacga cacgattatc
    17941 atcgacgaag cgcacgagcg cagcctgaac atcgacttcc ttttgggcta tttgaaacaa
    18001 ctcctgccgc gccgccccga tttgaaagtc atcatcacct cggcaacgat agacgcagaa
    18061 cgcttctccc gacacttcaa cggcgcgccc gttttagaag tgagcggacg gacgtatccc
    18121 gtcgaaatcc tctaccgacc gctgaccggc aaagacgaag acgacgcaga agtggagttg
    18181 accgacgcga ttgtcgatgc ggcggacgaa ttagcgcgac acggcgaagg cgatattttg
    18241 gtattcctgc cgggcgagcg cgaaatccgc gaaactgccg aagccctgcg caaatccacg
    18301 ctgcgccgca acgacgaaat cctgcccctg ttcgcacgcc tgtcgcacgc cgagcagcac
    18361 aaaatcttcc acccctcagg cgcgaaacgc cgcatcgtat tggcaaccaa cgtcgccgaa
    18421 acctcgctta ccgtgccggg catcaaatac gtcatcgaca ccggcctcgc gcgtgttaaa
    18481 cgctattccg cacgggcgaa agtggagcag cttcatatcg aaaaaatctc ccaagccgcc
    18541 gcccgccaac gatccggccg ctgcggacgc gtctccgcag gcgtgtgtat ccgactgttt
    18601 tcagaagaag attttaacag ccgccccgaa tttaccgacc ccgaaatcgt ccgcagcaac
    18661 ctcgccgccg tcatcctgcg catggcagca ttgaaactcg gcgatgtggc ggcattcccg
    18721 tttttagaaa tgcccgattc acggtatatc aatgacggtt ttcaggtgtt gttggagttg
    18781 ggggcggtgg aggccgtctg aaaacaggca gacataaaag aaaatccgcg tagagtgatg
    18841 taaacttacc cttgctttaa taagtagaaa atggtgggtt tacgtccccc cctgcggcta
    18901 ctaaaaaaat ataagagtaa acaacctttt tgaaagaaaa atgtatggac gaaattcaaa
    18961 tacccaaaaa agtggaatta caaaccaaac tagaaaatga aaagattgtt ttatcgaaag
    19021 gttctaccac gattattgtt ggtgctaatg gcacagggaa aacaagatta gctgtttata
    19081 ttgaagaaca attaaaggaa aaagcacaca gaatttcggc tcatagagca ttaaaattaa
    19141 accctaatgt caataaaata ccagaaaaga gtgccaaaac atatctatct tatggtcaga
    19201 actgggatgg aatcgatgta tcaaatagaa aaaattatag atgggataat aactcatata
    19261 ctcatttact caacgatttt gattggttat tacaatattt attcgctcaa caaaataata
    19321 ttgcggtagc aaataatcaa aagctcaacc gtaatgaaaa agtaaccaat tcaaaaacaa
    19381 agctagatat tttgcaagaa gcatgggaaa cattattacc acacagaaaa ttacatatta
    19441 cagcagatga tattcaagtc tctgctgtag ataatgagga attgtattct gcctcaaata
    19501 tgagtgatgg agagcgagca cttttctata ttcttggaca agttttgtca gtagatgacg
    19561 gttctgtctt aatttttgat gagcctgaat tacatattca taaatcaatt atttcaaatc
    19621 tatgggataa aattgaagaa ttacgacctg attgttcatt tctaatcatt acacacgata
    19681 ttgaatttgc tgcaactcga gtagctaaaa aatatgttat cagaaattat tatccgaccc
    19741 ctgcttggga tatttctgaa gttcctgaaa gtaattttga tgaagaaaca ataacgatga
    19801 ttttaggtag ccgtaagcca atattatttg ttgagggcaa caataatagt ttagatattg
    19861 ctacttaccg ctattgttat cctgattgga ccatcatacc caaaggggca tgcaaagatg
    19921 tcattcaatc agtatcatcg ctgaaaaaat taagtaatga aatgccatta ctaaacttaa
    19981 aatgttcagg tattgtcgat ttagatagta gggatgaaag agaaattgaa caattaaata
    20041 atttgggtat ttacatttta cctgtatccg aaattgaaaa tctttttagc ttaactgatg
    20101 tagcaaaaga gatattgaaa ctaaatcaat attcagatga agaattactc aataaactta
    20161 atggatttaa atccgaacta attaaatata tagataatga attaaaagac gataaattag
    20221 acgaatttgt tgtaaaacag gttcgacgta aaattgataa ttatttaaaa aatattgatt
    20281 tatcctccaa aataacaagt actgatatga aaaaatcatt acttaatgaa atttctactt
    20341 taacagaaca gaaaattgaa acatggattt cagaaattaa aaatgaaatt caaagatgta
    20401 ttgaacagca agatttggat aaattactta ctatatatga taataaagga ctcttggcta
    20461 aatcagcttg tgttttaaaa ggaatgcgta acaaacatga atttgaaagc tggataatga
    20521 gaacattaaa aggaaggaat aaagatttta ttgatgcaat cagacagaaa cttccaattc
    20581 tggattaaat aaaaccatct gaaaatttac cttcagatac agatatattt catgaaaaat
    20641 catcaaacta cactctcttt ccctacttcg agtagcctga aaccttgcgc agacaaacaa
    20701 ggcctgtctg aagaccgcag ccaataccgc ctgaccaaac tcggcgaaca aatggcgcac
    20761 ctgcctatcg acccgaaaat tgcgcgtatt ttgttagtat tattccgttt ttaaaaatgc
    20821 ccgattcgcg gtatatcaat gacggttttc aggtattgct ggaattgggg gcggtggagg
    20881 ccgtctgaaa ataaaatctt tctttataaa aaggcaggcc atgtttcatt ttcagacggc
    20941 ctaaatcatt gagaaactaa aaactattaa aaagggaata ttgggtttta aaactcaatc
    21001 ggtaaatttt tattgtgaaa tattaatgat gaaaaaatct ttccttacgc ttgttctgta
    21061 ttcgtcttta cttaccgcca gcgaaatcgc ctatcgcttt gtattcggaa ttgaaacctt
    21121 accggcggca aaaattgcgg aaacgtttgc gctgacattt gtgattgctg cgctgtatct
    21181 gtttgcgcgt tataaggtga cgcgtttgtt gattgcggtg ttttttgcgt tcagcattat
    21241 tgccaacaat gtacattatg cggtttatca aagctggatg acgggcatca attattggct
    21301 gatgctgaaa gagattaccg aagtcggcag tgcgggcgcg tcgatgttgg ataagttgtg
    21361 gctgcctgcg ttgtggggcg tgttggaagt catgttgttt tgcagccttg ccaagttcca
    21421 ccgtaagacg catttttctg ccgatatact gtttgccttc ctaatgctga tgattttcgt
    21481 gcgttcgttc gacacgaaac aagagcacgg tatttcgccc aaaccgacat acagccgcat
    21541 caaagccaat tatttcagct tcggttattt tgtcggacgc gtgttgccgt atcagttgtt
    21601 tgatttaagc aggattcccg cctttaagca gcctgctcca agcaaaatcg ggcagggcag
    21661 tgttcaaaat atcgtcctga ttatgggcga aagcgaaagc gcggcgcatt tgaagctgtt
    21721 tggctacgga cgcgaaactt cgccgttttt aacccggctg tcgcaagccg attttaagcc
    21781 gattgtgaaa caaagttatt ccgcaggctt tatgactgca gtgtccctgc ccagtttttt
    21841 caatgcgata ccgcacgcca acggcttgga acaaatcagc ggcggcgata ctaatatgtt
    21901 ccgcctcgcc aaagagcagg gctatgaaac gtatttttac agcgcacagg cggaaaacga
    21961 gatggcgatt ttgaacttaa tcggtaagaa atggatagac catctgattc agccgacgca
    22021 gcttggctac ggcaacggcg acaatatgcc cgatgagaag ctgctgccgc tgttcgacaa
    22081 aatcaatttg cagcagggca ggcattttat cgtgttgcac caacgcggtt cgcacgcccc
    22141 atacggcgca ttgttgcagc ctcaagataa agtattcggc gaagccgata ttgtggataa
    22201 gtacgacaac accatccaca aaaccgacca aatgattcaa accgtattcg agcagctgca
    22261 aaagcagcct gacggcaact ggctgtttgc ctatacctcc gatcatggcc agtatgttcg
    22321 ccaagatatc tacaatcaag gcacggtgca gcccgacagc tatctcgtgc cgctggtgtt
    22381 gtacagcccg gataaggccg tgcaacaggc tgccaaccag gcttttgcgc cttgcgagat
    22441 tgccttccat cagcagcttt caacgttcct gattcacacg ttgggctacg atatgccggt
    22501 ttcaggttgt cgcgaaggct cggtaacggg caacctgatt acgggtgatg caggcagctt
    22561 gaacattcgc gacggcaagg cggaatatgt ttatccgcaa tgagtggcgt aaaaaccaat
    22621 aaagacaaat ttagatgatg tcggggaaga tgcccgaccg acaagactat gcaaaatatg
    22681 aaaaaccaag tacgcggatc aggcatggat gcacgatcca atccggccaa tgtttcagac
    22741 ggcctgcaaa acagttcggg tcatatcggt accaacacgc gttaccgcct gaccaaactc
    22801 ggcgaacaaa tggcgcgcct gcccatcgac ccgaaaatcg cgcgcatttt gttggcggcg
    22861 aagaagcacg actgcatggc ggaaatattg gtgatcgcgt ccgcgctgtc gattcaagac
    22921 ccgcgcgagc ggccgctgga agcgcgcgat gccgccgcca aggcgcacga gcgttttacc
    22981 gacaagcaat ctgactttct tgcctatctg aacatttggg acagcttcca gcgcgagcgc
    23041 gacaagggct tgtccaacaa gcagttggtg cagtggtgcc gtcaatattt cctgtcgcac
    23101 ctgcggatgc gcgagtggcg cgagctgcac caccagcttg cccaaaccgc gattgaaatg
    23161 ggtttgacca ccaaggaagc ggctttcaga cgactttccg aaatcaagca gcttacgtcg
    23221 tctgaaaacc aaggcgatca agacttatcc gccaaacgca aacaaaaaca actggataag
    23281 aaacaacacc gcgcccaaat ccgcgccgcc aaagaagcgg gctacgaaca aatccaccgc
    23341 gccctgctca ccggccttat cgccaacgtc ggcatgaaat cgcccgacgg caacgactat
    23401 accggcgcgc gcggcagccg tttccacctt ttccccgcct ccgccctgtt caaagccaaa
    23461 cccaaatggg tgatggcggc agaactcgtt gaaacgacaa aactttatgc gcgcgacgtc
    23521 gccgttatcc agcccgaatg gattgagcag gaagccccgc acctcgtccg ctaccactat
    23581 ttcgagccgc actgggaaca aaaacgcggc gaagtcgtcg ccagcgaacg cgtgacgctt
    23641 tacggcctga ccgtattgcc gcgccgcccc gtgccttacg gcaaagttgc gcccgaagaa
    23701 gcacgtgaaa tctttatccg cagcgcgttg gtggcgcagg aatgcgattt gaaagcggat
    23761 ttttttgtcc acaataaaaa gctgattaaa gaaattaccg aactcgaaca caaatcgcgc
    23821 aagcaagacg tattggtcga cgacgaagcc ctgtttgcgt tttataacga acgcctgccc
    23881 gaaatggctt ggaaagacgc gcaaggcagc gtttggggaa gtgaagattc cgtacggatt
    23941 attgaatctg acaaagccga gaggtcgtct gaaaatgagc gcaacgagtt tcgtaaaaac
    24001 aagcgtaatg ggtctcgcca aaatgaaaat cacggcaaca ccgtaggttg ggttgaaaac
    24061 ccaacatcag ccgcaactgc aaaaactgtt gggtttgaca acccaaccta cgctactcag
    24121 caacccaccc cctcccccgc gagggagggt cggggagagg gcaaaacagt tgccgcacaa
    24181 accaactttt ccgcaaccgc agcaaaccct ctcccgcagg agagggagca gagtgccgca
    24241 gcttcagcgg tttcaaacga cctgcatcct gcaaatcccc aacaaaccgc cccctccccc
    24301 gtgggggagg gtcggggaga gggcaaaaca gttgcctctc aaaccaactt ctccgcaacc
    24361 acagcaaacc ctctccctaa ccctctccca caggaggggg aacagagtgc cgcagcttca
    24421 gcggtttcaa acgaccctca accccaaaag cagccagcac cccaaaaaga ccgtctgaaa
    24481 cccctccccc tcgccgacat ccgcaccttc caagcctggc tcaaaaccgc cgaacgcgag
    24541 aacccgcgcc tgctgttcct cagccgcgac gatttgatgc aacacgccgc cgcacacatt
    24601 accgaagagc aattccccaa attctggcaa accgcagacg gcaagttcaa actttcctac
    24661 cgcttcgagc cgcaccatcc gctcgacggc gttaccctca ccctaccgct gaccgtcctc
    24721 aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gtatgttgcg cgaaaaaatc
    24781 cagctgctga ttaaagcact gcccaagcaa atccgccgca tttgcgtgcc cgtacccgat
    24841 ttcatcacca aatttctcga aagcaatccc gaccgccaag cggtcattat tccccagttg
    24901 gcgcacttta tcgccaaaag cgcaggcgat atgcggattt tcgagcaaat cgaccaagac
    24961 gcatgggcgg cgcaagaatt gcccgaacac tgctatctga atctgctcat catcgacgac
    25021 ggcggacaag agcttgccgg cggccgcaaa ctgcacgaat tgcaacaaca gctcggccaa
    25081 gccgccgccg tcaccttccg cgacaacacc caagaattcg agcgcgacaa cgttaccaca
    25141 tgggatatcg gtaccctgcc cgaatccatc aaattcgccc gcggcaaaca acagctcacc
    25201 ggctacctcg gtctgcaaaa agaaaaagac ggccgcatcg ccctgcgcct gtgtgacacc
    25261 accgaagctg ccgaacaggc acaccgtcaa ggcgtcatcg aattgatgaa gctgcaatta
    25321 aaagagcagg taaaagacct gaacaaaggc atccaaggct ttacccaagc cgccatgctg
    25381 ctcaaacaca tcaacgccga caccctgcgc gacgacctca cccaagccgt ttgtgaccgc
    25441 gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga acaaatcaaa
    25501 cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctacct gcaggaaacc
    25561 gccgccgcct acgccgaact gaacggcaaa ctcggcaaac acccattgac ccaccttcta
    25621 agactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgcacccc gtgggcacaa
    25681 tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa atacagcagc
    25741 aaccccgccc gcgacgcagc ccgcgaagcc gatatacagg aattggaaca aatgtggcag
    25801 gaaaaaaacg acggtttggt gaaacaagga ctacccgttt cagacgacct caccgcgttt
    25861 aaatggatga tcgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa gacaccgtat
    25921 ccggtgtcgg tgaagcggct gttgaaagag tgggaggatc ttaattaaag gaagatgccg
    25981 tctaattttt tcagacggca tcttttattt ccctgaccaa ccgatttaat cgctccctac
    26041 aaatagcttt attttttaat tcgcactccc aaacgacaat cactttgaaa ccgatctttt
    26101 ccagctcggt ttcattttta atatcacgtt cgcaattttt tgtgattttt tccaaccaaa
    26161 aatccgtatt gcttttcggg atatgtcctt tattacaaga atgcccgtac caaaaacagc
    26221 cgtgtataaa cacgactgtt ttatatttcg acaaaacgat atccggtttg cctacatagc
    26281 gtttatcgtt tttccgataa cggaaacctt ggaaaaaaag aaatttcctt accaatactt
    26341 ccggtttggt ttctttgcta tggatttttg acatcacgaa agaacgtttg gatgtagtga
    26401 aaatatcggt cataaattac ttggaagata ccaccttcga tcgttttttt gtctgttcca
    26461 cttttcttaa caaatacgaa accaagctta ttaaatccaa agcatcctta tcattaaaaa
    26521 tactaggaga taaatttgct cttaactcat gttgtacagg atttctaaat ccagtccatg
    26581 ttccgcatgc aagctgttca agaccatctt ctaaattttg ttccgccttc gttttgttat
    26641 tcgttaatag aagtatagaa tttttactcc cgaaagcttg ttcaatcaat tctcttccgt
    26701 ctatgctatg aagacctgtt tgcttctgaa ccttatcttc gaaaacttta actgcctcga
    26761 ttgcagcact taaataatcc ttcttttcat tgtaaaaatc attacaggca gtatgcaaat
    26821 cctgatgcaa atgccgccaa tgatagtaag gatattctgg aataatacta tgtaaggcag
    26881 agattacttc cgcttgctct ttatttgtaa gctccgaatc ttccaaacga ttcaaaataa
    26941 cttctactgg ttccctttcc ttttctggca atttgttttt ccaatcttcg atatcaactc
    27001 cgctttcgcc tttcacttcg tcatctttct ctttttttct tctttttctc caatcttgcc
    27061 cgatggctcg gacgatctcc ccaaggaaaa cacgtaattt tgaggttttt gaatgcttcc
    27121 agtctaatga tgtcctgtcg gtagcaatta aatcttcatc gtcattatct ataaagtcca
    27181 cattcagcca tccagtcaaa tatgaataga aatggctgga ttcgctatca gtgaaaaact
    27241 cgggcatatt taccattctg ccgttggcaa acaaggtaat acctttcata ttattcttta
    27301 aaggtttttc cgttgtaatg aacttacctt gtattaatcc gctgtattca tactgttcga
    27361 attttgaaga taacgatgaa atattggttg ctaaatcctg gtattcccat tcgaattgtg
    27421 gggtaacaag atcatatttt agatttccat caatgatttt aggttcagag ccgttcaagc
    27481 ttactttgat tttaaaatcc tgagccggaa aatcaaacaa gcgggaaaga tgctctacat
    27541 aattatctaa cggatatcct tgctttttcg tcagttcggt taaagttatg gttgtcccgc
    27601 tttcgatatt ggattcaaca gattcttttc gaaactccgg ttgataaata cccttgcttc
    27661 ttcgaatctc tgcataatcc aaagtaaaag taaccctttc gtttccctgg atagtagaaa
    27721 tttcaatttt gttgccaagc ccgaataatg ccaatttacc aaggcctttt ttacccgttg
    27781 gaattcttcc gcacggggag gcttgttttt cttcccttct gttccgaccg attctcaaat
    27841 aaaaatcatt gatttcatcg aagctcattc ctataccatt atctttaata acgattttat
    27901 gctccggttt atcgaataac ctaacttcca cttccgtagc acatgcatcg taggcattcg
    27961 ctatcaactc ggcaatcgca ggaacagtgt gcgaatacat ctttacaccc aaatgttcga
    28021 ttacggttgg ctcgaaactc attttcaatt tttcttcaga catgttttct ccttttctaa
    28081 cattcgatat tatcaacaat tgccctgcct attttttccg catacatcgg aggaacggca
    28141 ttaccaatca aacgggctat cttgtccctg ctgcccgctt taaagacata gttacgaggg
    28201 aacgattgca atgttgcacc ttcgcgcagt gataacgccc tatcttcttc aggatgggca
    28261 aacctcccgt tggaaatact aaagaatttg gtcgttatgg taggtgcggg cttatcccac
    28321 catagcctac caaacgtgtc tttaaaacta ttgtcttttc cgataaaaca ttccagctgt
    28381 aattcgggaa tatgcgcaaa agccaaacgg tttcctccgt ttttaggtat caaagccaat
    28441 cgtttcaagt tgatatcaga taatcccgca cagctatgca taaaatccgt ttcgtcttgg
    28501 tgtcctgcaa taattttggg aaagccattt tccattccca aaacatcgcg taccgtaagc
    28561 cgtttgcccg aatacttgac tggttccagc ttttctttgg ttattctgtt tgcaattaac
    28621 gtaaatcttt tacggctttg gggaattccg taatcagccg tattgtgaat accgaaatgg
    28681 acggtaaatc ctttttcttc caataacttt atgaaattat caagcctgct ttctttcatt
    28741 ctgctcagaa ttcccggtac gttctctaca acaacatatc cgggattgaa atattctaca
    28801 aaccgctgaa actccaaaag caaactcttg gatttttccg attttctttt gtctgtttga
    28861 ataacactcc aatattgaca aggactacaa cctataagaa tcaaatcatc atcattcttt
    28921 tttaacccga gttctttttc caaggtttca ggctgcaatt caaaaacatc agcctgtata
    28981 aatttggcat ttatattggc ttcatatgtt tctttgcagc tgggttcgta atctatcccg
    29041 gctaaaacct gaataccggc ggattgcatt ccatagctca ttccacctcc gccacagaaa
    29101 aaatcaaccg cctttaactt actgtttttc atttctattt aaataattag actagtgaga
    29161 actaacttta tataacatat aatttattgc ttatcaaaaa taatttgaga ttttattaaa
    29221 attcatgcat ctaaataagc agaacttaat ttcctccaaa cgagcggatg tcttcaaatg
    29281 cacacttcac agaaaggtcg tctgaaaccc attttcagac gacctttttc cacttcccaa
    29341 aacaagaatt cccaccccct ccccaaacgt cgcccgaacc agtaaactcc ccatatttca
    29401 acaacttctt ggaagccaac catgccccgt atccaccctc ctcacctgtt cccgccccat
    29461 atcgccgaac gcggcctttt gtattttcag cagggcaagg ttcgcgatgt ccgaaaaact
    29521 tccgccgggc gttatcgggc ggaggtatgc ggtttggaaa actattgggt atggctgaag
    29581 ctggatagtg atttgtatat taaagacgaa agctgcgatt gcccttatgt ctaaaggtgc
    29641 aaacatacct taaattacta tattacatag gcaaaataca agcctataac gaattggaaa
    29701 caaaatgccg tctgaaaaca tcttcagacg gcattataaa atctgttcac cttttcagat
    29761 gagtaatgta cacccctata caatttttgc tactataccc cataaatcca cggctaaaga
    29821 tatccttatt atgtcctatg atttatcgaa acgacttgta atcggcttag catcaagtgc
    29881 cctattcgac ttatccgaat cggataatat atttagaatg gaaggggcag aaacctatag
    29941 gcaatatcag agagaaaaac aaaaccatcc cctaaaaaag gcgttgtctt tccatttatt
    30001 aaaaaacttc tgtcaatcaa tgaaataaac ccaaacgacc caacgattgg gtttattctt
    30061 ttatccagaa acaatccaga tacagattac gagtcataac tataggctta atattacacg
    30121 attttcattc catcaaggcg gaaaaccgca caaatactga aacactatcg atcgatttgt
    30181 aaacaagcct acttaagtaa cttgcagtcc ttatcatttc ctttaaaata atccagcccg
    30241 tcactacacg aactggcgga cttcttgcaa ataaaggtta ctagattttc attcatctta
    30301 ataataaaag gatttttatc tttatctatg gctaccgcct tcaacatgaa tttactgtct
    30361 aaggccccgc gcgcgattcc attcaaacgg atacaaaagc cttctgcctc tttaatcggc
    30421 aaacttggcc atttggtaga tgtttgttta aatctcccat tctgcagata aaacttttcc
    30481 ataaaatgtg cattttctaa caaggctgcc cgcactgtat ttatctttgc tttctcaaca
    30541 taattgcgat agctcggata aacaattaaa gcaagtacag acaatatcaa gaccactgat
    30601 attaattcaa ccagcgtaaa cccccgatta tcagtcatta ctttacttcc aataagaaca
    30661 gattattcaa catatttctt tgaacagact tactatccca ttcagcagta tgcatatttc
    30721 ccactctatt ttttatccgc gggcagcact ggtttagc
//
LOCUS       DAWKOD010000006        29789 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000006 DAWKOD010000000
VERSION     DAWKOD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.006"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(249..896)
                     /locus_tag="WJK73_000183"
     CDS             complement(249..896)
                     /locus_tag="WJK73_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ7556727.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(893..1261)
                     /locus_tag="WJK73_000184"
     CDS             complement(893..1261)
                     /locus_tag="WJK73_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ7556728.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1271..1942)
                     /locus_tag="WJK73_000185"
     CDS             complement(1271..1942)
                     /locus_tag="WJK73_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ7556729.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2107..2826)
                     /locus_tag="WJK73_000186"
     CDS             complement(2107..2826)
                     /locus_tag="WJK73_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7556730.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALLPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2909..4222)
                     /locus_tag="WJK73_000187"
     CDS             complement(2909..4222)
                     /locus_tag="WJK73_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ7556731.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSATVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRTLPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFSNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(4301..5173)
                     /gene="hpnD"
                     /locus_tag="WJK73_000188"
     CDS             complement(4301..5173)
                     /gene="hpnD"
                     /locus_tag="WJK73_000188"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ7556732.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQKKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGEMPEHPVNQALQQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5344..7206
                     /gene="hscA"
                     /locus_tag="WJK73_000189"
     CDS             5344..7206
                     /gene="hscA"
                     /locus_tag="WJK73_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ7556733.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7288..7944
                     /locus_tag="WJK73_000190"
     CDS             7288..7944
                     /locus_tag="WJK73_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7556734.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            7980..8741
                     /locus_tag="WJK73_000191"
     CDS             7980..8741
                     /locus_tag="WJK73_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ7556735.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            8796..9137
                     /gene="fdx"
                     /locus_tag="WJK73_000192"
     CDS             8796..9137
                     /gene="fdx"
                     /locus_tag="WJK73_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ7556736.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9424..10011)
                     /locus_tag="WJK73_000193"
     CDS             complement(9424..10011)
                     /locus_tag="WJK73_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ7556737.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10224..10421
                     /gene="iscX"
                     /locus_tag="WJK73_000194"
     CDS             10224..10421
                     /gene="iscX"
                     /locus_tag="WJK73_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ7556738.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10489..10890)
                     /locus_tag="WJK73_000195"
     CDS             complement(10489..10890)
                     /locus_tag="WJK73_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7556739.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            11101..12060
                     /locus_tag="WJK73_000196"
     CDS             11101..12060
                     /locus_tag="WJK73_000196"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ7556740.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12128..13438
                     /gene="tilS"
                     /locus_tag="WJK73_000197"
     CDS             12128..13438
                     /gene="tilS"
                     /locus_tag="WJK73_000197"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ7556741.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            13512..14297
                     /locus_tag="WJK73_000198"
     CDS             13512..14297
                     /locus_tag="WJK73_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7556742.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14307..14504
                     /locus_tag="WJK73_000199"
     CDS             14307..14504
                     /locus_tag="WJK73_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556743.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            14766..15119
                     /locus_tag="WJK73_000200"
     CDS             14766..15119
                     /locus_tag="WJK73_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556744.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15131..15436
                     /locus_tag="WJK73_000201"
     CDS             15131..15436
                     /locus_tag="WJK73_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556745.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            15498..16874
                     /gene="mpl"
                     /locus_tag="WJK73_000202"
     CDS             15498..16874
                     /gene="mpl"
                     /locus_tag="WJK73_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ7556746.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLSENVL
                     RHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            17081..18133
                     /gene="bioB"
                     /locus_tag="WJK73_000203"
     CDS             17081..18133
                     /gene="bioB"
                     /locus_tag="WJK73_000203"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ7556747.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18211..18846)
                     /locus_tag="WJK73_000204"
     CDS             complement(18211..18846)
                     /locus_tag="WJK73_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7556748.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19169..19429
                     /locus_tag="WJK73_000205"
     CDS             19169..19429
                     /locus_tag="WJK73_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7556749.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19492..21351)
                     /gene="ilvD"
                     /locus_tag="WJK73_000206"
     CDS             complement(19492..21351)
                     /gene="ilvD"
                     /locus_tag="WJK73_000206"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ7556750.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21524..23293)
                     /gene="cysI"
                     /locus_tag="WJK73_000207"
     CDS             complement(21524..23293)
                     /gene="cysI"
                     /locus_tag="WJK73_000207"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ7556751.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARKNSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23320..25134)
                     /locus_tag="WJK73_000208"
     CDS             complement(23320..25134)
                     /locus_tag="WJK73_000208"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ7556752.1"
                     /translation="MSEHDMQNINPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQAMPPADLQTVPEGRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADCVEEDGTVRVFVERNDGF
                     RLPEDKSKPIVMIGSGTGIAPFRAFLQQRAAENATGKNWLFFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25131..26417)
                     /locus_tag="WJK73_000209"
     CDS             complement(25131..26417)
                     /locus_tag="WJK73_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ7556753.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGEDEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVEQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTLPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26456..27379)
                     /gene="cysD"
                     /locus_tag="WJK73_000210"
     CDS             complement(26456..27379)
                     /gene="cysD"
                     /locus_tag="WJK73_000210"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ7556754.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTLLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27417..28157)
                     /locus_tag="WJK73_000211"
     CDS             complement(27417..28157)
                     /locus_tag="WJK73_000211"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ7556755.1"
                     /translation="METTLFKPALWQIPHIGSGGETALTEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28168..29619)
                     /gene="cobA"
                     /locus_tag="WJK73_000212"
     CDS             complement(28168..29619)
                     /gene="cobA"
                     /locus_tag="WJK73_000212"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7556756.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFATLAKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGE
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         6410 a         7832 c         8572 g         6975 t
ORIGIN      
        1 tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
       61 ccactatacc atacaaccac gccggaatta agtttaaatt tgaataaaag gttcgggttc
      121 tgcaaaatac agaacccgaa ccttgttcgg atattgaaac cggccgcccg attttgggcg
      181 gtgcggcttg caagtatcaa gattcgcata tgccgtctga agctcggaga ggttcagacg
      241 gcatatgctt atttgggctg ctcttcgacg aatctcggac ctttcaagat gccgttgtga
      301 gaatagggcg acagcaggtt gtatgcggcg gttttggaaa cctgataacc gcggtcggtc
      361 aggctgttgg caatctgatt gaccactgcg ctgaccaaag cccccaacag gccgctgttg
      421 ctgttgttgc tgccttcgcg gatgctggcc gaacccgacc acaactcttt tccgttgcgg
      481 gaatcgacca gccgtgcttt ggcggatacg gtcgtcacgc tgtctaaaat ttgatatgaa
      541 gtgccgtatt cggtaaccgt aatgtacaaa accgcatcat tgccgaaaat ctgatgcagt
      601 ttttccggcc ggacggcgtg aatatcggcg gcattggtca agccgttttg tttgaaggtt
      661 tcctccacga ctgcggcggg gaagacgtaa tagccggctt cggaaagcgg agcggcggtc
      721 gaagccagta caccccatgt tccgttgaca tcgggcgatt cgttcagcgg cggaaccacc
      781 aaaattgaag ccggtttgct ttccttgaat gacgtgtagt cgaaatcggg cgctttttga
      841 acttggcagg cagacagcgc caacacggcg gcaagcccta aaatcaaagg tttcatcgct
      901 tgcctccttt acctgttttc atcaggaagt ccataaatac gcccgattcg ggaaacagcc
      961 ttttctcttc ttcaaactgg cggaacgcgc cctctttgtc tcccgaacgg gaaagcagca
     1021 gtcccagatg ggcgtgcgca cccggggcgg cattcatttt tttgttgccg gcttccacaa
     1081 agtatttttc catcttttcg gtctgcttgc ccaacgaagt gtcgtcgttt ttcaaacctt
     1141 catagacggt atcgggatag ccgccgtaat aatacaggga tttttgcccg ttgccgccgc
     1201 aggcggtcag agccaagacc gccgcacaca gcgacaaacg gctcaaggtt ttcggattca
     1261 tcatttctcc ttaacggttg ggttgccatg cgccgttgtc aacagcctga accaggctgt
     1321 tgacggcttc gcggattgcc aagtctaaaa ctttgccgtt caaagtcgca tcgtagccgg
     1381 aagtgccgcc gaaaccgatg atttcacggt tggaaagtgc gtattcgccc gcgccctgtg
     1441 cggaatagac gatttcggaa gtattgacgt tgacgatatt cagagccact tttgcatagg
     1501 cgatttgcga tttgccgcga cccaaaatgc cgaagagctg atgatcgccg acatctctgc
     1561 gtccgaattc ggttacatcg ccggtaacga cataatctgc gcctttcagg ttatgcgctt
     1621 tgccggaaat gccggattcc tgttttaatg cgatcaaatt ggtgcggttc agtacgttga
     1681 agcggttggt ctgttgcagg tgcgttacca gaatggtttt tgcctggctg cccaaacggt
     1741 cttccccgtc ggagaaaatg cctttttgga agctggagcg gttgtcgaat gttccgacgg
     1801 aaatcggggt acgaacaccg tgatattgcg tattgtagga ggcgactttt gctacctcga
     1861 gactgcgtga ggattcggtc gcacagccgg tcagtgaaac ggcagcggcg gcaaggacaa
     1921 cggcggtgga aacggttttc ataaaattta ccctaaggtc aagttaagga aataacgggt
     1981 tgtcattatt gtccttatgt aaatttaagt caaggtgttt gtctgtgcgg gacggatgcg
     2041 cacggaaggt acggatattt ttcaaacgga atcgcggcgc gggcgggatg cctgcctttt
     2101 ccggatttag aggtaaacga tttcgccgct ccgccctttg ctttcggcac ttgcccacca
     2161 gacaaatgcg ggcagcacgt ccccgtagct tttgcgttcg cttttggctt cgcccggatg
     2221 ggatttgatg cgttgcgggg aattgatggg tccggggacg aggacgttgg cgcgcaggtt
     2281 gccgaagcgt tcccattcgt cggcggcgac tttgcacagg tagttcaacg cggctttgga
     2341 cgcgccgaag ccgccccagt aggctttggg ggtttccccg tggctttcgc cgacgaagat
     2401 aacggatgcg tccggcgact gtttcagcag gggaaggagg gcgcgggtca gccccatcgg
     2461 ggcgacggtg ttgatgcggt attggttgac ccattcggcg acggtttgga aatccagcgg
     2521 cgagagcgcg taaaaatagc cggcgcagtg gacgatgccg tccagtttgc cttgcgtggc
     2581 ttcggcaatg gtggcggcga aatgttcaaa ttctttttct tccgcgctaa taaggtcaaa
     2641 gcagatggcg aatggttcgg ggtatccggc ttcgacaatc gcgtcataca ctttttccag
     2701 ttttttctga tgacgggcaa ccaaaatcac ggttgcgcct gccgccgcat aggctttggc
     2761 gacctgttcg cccagacctt gcgatgcgcc ggttactaag atggttttgt cggacagtgt
     2821 cgccatactt tttccttttt ggtggtcggt taaggtattt tagcgttttg ccgcaccttg
     2881 taaagcgtcc cgataccggc cggcgttttc agacggcatc gctcaggctt tgcaggcagg
     2941 cttccgccga cgcgaaacct gattgtacgg cggcttcgag cgtggcgggg tagtctgggt
     3001 ggaggtagtc gccggcgggg aagatgcggt gccggtgcaa ccacgacaaa tccggcggcg
     3061 gggcatcggc tgcggttgtg gcgcgttttt cggtgatgac gcgcacggct tcgggttcgc
     3121 ccaaatgcgg aaggatgcgt ttgaggtcgg cgtgaacttt gtccgcccac gcccggtttg
     3181 aaaacgcgcc gacgcggtcg gaaacgctga tgacggcgga cacttcgttt tcaggcagtc
     3241 cgagcctgcc ccggcaaagc agccattgca ccgtgccgtc ggcaaggccg gtcagcgggg
     3301 cgggcaggcg gacgggttcg gcgtagcgca gatagacggt ggtgatggcg tggtagcgaa
     3361 ggttttgata tgccgtctga acgtgttcgg gcgtgccttc gggcaggagc gcggcggcgt
     3421 ggtagggcgc ggtggcgggg acggcggcat cgaaagcttc gccgttgacg agcactttcc
     3481 cgtccgggag ggtgttcaga cggcatacgc gcgtttcgag gcggatgtcc gcgccgagcc
     3541 gttgaagctc cgccaaggcg ggttcggcga cgattgcgcc caaatcctgc ttgggtagga
     3601 gatagtcgct gccggatttt ttcgtcagca cgccgtcgga caaaacgttg cacaacacgc
     3661 gcaggcttgc ggtttccaaa ggcgtgttga gcgcacccca caccagcggc tgccaaaact
     3721 gcatcacggc ggcacgcggc acgttccgct gtttcagcca ttgtgcaact gtcgtgtcgg
     3781 gctgtccgag gcgtgcggac ttctgcaaat cggacatatc ggcaagcagt ttggctttga
     3841 atgcggacgg tacgcgccgg gcaagcagca cgccgcccaa aatatgcagc ggcgcgggca
     3901 gggggagggt gcggaactgc aaaccgccgt gcatatgcca gtgcagcggt acgcgcaaaa
     3961 aggcggcacg ggggtctgaa ccgatggttt tcatcaggcg caacacgccc cggtatgcgc
     4021 cgagcaaaat gtgctgcccg ttgtccaaaa aaccgaaacc gtcggtattt ccggccagtg
     4081 cgcgcgccct gccgcccgcc tgccggccgg cttcaaacag ggtaacgtcg gcgtgccgcg
     4141 ccaaggtgac ggtggcggac agtcctgccc agcctgcgcc gatgacggcg attttcgggc
     4201 gcggatgcgg cgtgttcatc atttattcct ccaatggttg aaaccccgtt tttcggggcg
     4261 gcgggggttg ggttttcctg aagggcgggc ggtatgcgcc ttatgcccgt tccggcgtgc
     4321 cggggcgcgg tttgaatccg aataaccagg ttttcagggc aatgcgtttt ttgcgcggcg
     4381 aagggagggc gattttgtat ttgaggacgt tttgcgcgcc gtctcggtcg atttcgttta
     4441 aaagcgcgta ataaaccgcc gccataacca gtccgacttt ttgggctttt ttgtcggcat
     4501 caggcagcag cgatacggct tcgcggtagg tttcacgcgc acgctttatt tggaatgcca
     4561 tcaattcggt gaaattttct gtcggccggc actgcaaaat cacacttgcg gggacatcaa
     4621 accgccgcat ttcctccatc ggcaggtaaa tccgcccatt acgggcgtct tcgccgacat
     4681 cgcggatgat gttggtcagt tgcagcgcaa gtcccatttt gtcggcgtat tccagtgttt
     4741 tgctgtctga aaaccccaaa atccgcgcaa tcaggcagcc gactacgcct gcgacgcggt
     4801 ggcaatacag tttcaattct tcaaaactgc cgtaacgggc ttgaaccaaa tccatctgca
     4861 tcccgtcgat taaggcttcc agttcatatt tcggcagctt gaaggtttcc ttaacctgct
     4921 gcaaggcctg attgacgggg tgttccggca tctcgccacc gaacaccttg tccaaatcgc
     4981 cgcgccacca gttcaatgtt gcctgtgcaa catcggggtt ggaacattcg tcaaccacat
     5041 cgtccaattc gcggcaaaaa gcatataaaa ccgttaccgc atcccgtttt ttctgagtca
     5101 ggaaacggaa gcccgataaa aaactggagc ggctttcttc tgctttttga cggcaatagt
     5161 cgagtccttt cacgatttat attcctaatg atgggcgaga aagacggatt ttatcggcat
     5221 ttggcggtag agggcaattt cggcggcacg gcccaatcct tagcggtttg ctcaactatc
     5281 ggcgcaaatt ctgttaaaat gccgacttcc ttcctttaca caccgcaccg acaggcagaa
     5341 tttatggctc ttttgcagat ttcagaaccg ggtatgtccg ccgccccgca ccgacaccgt
     5401 ttggcggcag gcatcgattt gggtacgacc aacagcttgg ttgccaccgt ccgcagcggc
     5461 agtgccgcct gcctgcccga tgccgaaggg cgcgttaccc tgccttccgt cgtccgctat
     5521 ctggaaaacg gcggcattga agtcggcaaa accgccctgt ccgcccaaaa aaccgacccg
     5581 ctgaacaccg ttagctccgc caaacgcctt atcgggcgga cgcttgccga tctgcatcaa
     5641 aatacgcatt acctgcctta ccgtttcggc gacaatcaac gcgttatcga actgcatacg
     5701 cggcaggggg tgaaaacgcc tgtcgaagtg tcggcggaaa tcctcaaaac ccttaaatcg
     5761 cgcgccgaag aaaccttggg cggcgatttg gtcggcgtgg tgattaccgt ccctgcctat
     5821 ttcgatgatg cccaacgcca agccaccaaa gatgccgcgc gtctggcggg tttgaacgta
     5881 ttgcgcctgc tcaacgaacc caccgctgcc gcaatcgcct acgggctgga caacgcctcg
     5941 gaaggcacgt ttgtcgtgta cgacttaggg ggcggcacat tcgacgtatc cgtattgcaa
     6001 ctgaccaaag gactgtttga agtcaaagcc accggcggca acagcgcgtt gggcggcgac
     6061 gatttcgacc accgcctgtt ctgccgcctg ctcgaacaaa acggactctc ccaactcaac
     6121 gaacaagaca gccaactcct gctctcgctc gtccgcgccg ccaaagaaca attaaccacg
     6181 caaaccgaag cgcgcattca ggcgacgctt tcagacggca tggcaatcga cacaagcatc
     6241 agtcgcgccg agttccacaa cctgacgcag catttggtga tgaaaacgct cgaaccggtc
     6301 acacaggcgt tgaaagatgc cggtgtcggt aaaaacgaag tcaaaggcgt gattatggtc
     6361 ggcggttcga cccgtatgct gcacgtccaa caggcagtcg ccaccttctt cggacaaacc
     6421 ccgctgaaca acctcaatcc cgacgaagtc gtcgcgctcg gcgccgccat acaggcaaac
     6481 gtcctcgcag gcaacaaaac cgacggcgaa tggctgctgc tggacgttac acctttgtcg
     6541 ctcggtttgg aaacctacgg cggcttggcg gaaaaaatca tcccgcgcaa ttccaccatc
     6601 cccaccgcgc gcgcgcagga ctttaccacc ttcaaagacg gtcaaaccgc gatgacgata
     6661 cacgtcgtgc aaggcgagcg cgagctcgtt tctgactgcc gcagccttgc caaattcacc
     6721 ctgcgcggca ttccaccgat ggcggcgggt gcggcgcgta tccgcgtaac cttccaaatc
     6781 gatgcggacg ggctgctgtc cgtttccgcc caagaacaaa gcaccggcgt acaggcgcaa
     6841 atcgaagtca aaccttccta cggcttggac gacggcgcga ttacccgaat gcttaaagac
     6901 agtatggaca acgccgccga agatatggcg gcacgcgccc gcgccgaagc cgtagtcgaa
     6961 gccgaaagcc tgaccgatgc cgtcaacgcc gccctcgagt tggacagcga tttactggat
     7021 gccaaagaat tgcaacagat ccggcaaggc atcgccgatt tgcaaggccg tctgaaagac
     7081 ggaaaagccg aagacatccg ctccgccgtc gccaaactca gccgcagcac cgacaacttc
     7141 gccgccaaac gcatgaaccg caatatccaa cgcgcgttga ctggtcaaag cgttgataat
     7201 atttgacggc aaatggtttc agacggcatt ggataaagag gtcgtctgaa aaaatatcat
     7261 ctcacagaaa taaaataaaa aacaacaatg gaaaagtcag gcattgtcta tctgatgaaa
     7321 accgtcatca agggcgtgta taaaatcggc atttcggatg taagcaattt tgaaggcaga
     7381 atgcgccatt tggaaaacaa cggttatgcg aacgttgccg ggttggaacg catcctcgcc
     7441 gtcaaaaccg acaattacaa agaaaaagaa aacctgctcc atgaaatttt tagtaaaagc
     7501 aggataggcg ataccgaatt gttcgccgtg gacgagaatc ttgtaaaacg tttgttttta
     7561 tcgcttcgcg gcgaaatcgt gttcccgaaa aacgaaacgg cggaatcgga atttgaaaaa
     7621 agcgtccacg aacgcaggca ggaagggaat gccggatccg gtcgcaaaca actgcttgat
     7681 ttggtacggc gcggacaccg ggaataccct tacgcgctgc cccggctttt ggcgggcgcg
     7741 gcatcctaca agccgaaaaa atcgaaaatc cgccttttta aagaagcata tttacgcaaa
     7801 agcggtacga ggctgaccga cgaaattgca gacggcatcc atatttacac ctgtttttcg
     7861 cgggcggatt tggaaaaagc ctattccgaa tatttggaac ttttcaaatc cgaatcggat
     7921 gccgaaggca gaaaaccacg gtaaggtgca aacagatacc gtacacgttg aggagcagta
     7981 tgatgggcga ttccgtcatt tattatgtag aacaggcaga cgaaccggta aaccgtgccg
     8041 gcgaacgcgc ccgtaaaaca ttcaaatact tttggcgcga gcttttttgg gaacgccgcc
     8101 gcatcattcc cgccttagat tttgccatgg tcaaagcccc ttttttccaa gacggcgaag
     8161 acggcgaaat ttgcgaacat atgtggatcg acgatattta ttttgacggt ctttacattt
     8221 acggtgtgct gaacaataaa cccggcgaac tgactaatgt cgaacaaggc gaaagcattt
     8281 gcgttccggt tgacgacatc agcgactgga tgttcgtgtg caacggcatt ccctacggcg
     8341 gctttaccat acaggcaatg cgtggacaga tgacggaaga ggagcgtacc gaacacgatg
     8401 ccgcatgggg aatcgatttc ggcgatcccg ggcaggtatt gctggtgtat gaagaaaaag
     8461 aacatcccga aaatctggaa gagcatccga tgtgccggaa ctgtattgac gattttcggc
     8521 aacagttgtc ccaaaatccg gattttctgc atgagcagga cgaagacggc tatacgccgc
     8581 ttcatcatga agccatcgca ggaaatgcac ttatggttca agccatgctt gaatacggcg
     8641 caaatcctgc ctcaaagaca tcggaaggct ataccgccct cgattttgcc cgcctgacgg
     8701 gctggcaaaa tgttgccgac ctgctcgaac cgcgacatta ggcagacagt tttccgaaaa
     8761 cgaacacaaa cactttttac agaaagacaa taaaaatgcc caaaatcacc gtacttccac
     8821 acacgacatt atgccccgag ggtgcagtca tcgataacgc acccgaaggt aaaaccgtcc
     8881 ttgacgtgct gctcgaccat gatatcgaag tcgatcacgc ctgcgaaaaa tcctgcgcct
     8941 gcacaacctg ccacgtgatt atccgcaaag gtttcgacag tctggaagag ccgaccgaat
     9001 tggaagaaga cctgctcgat caggcttggg gtttggaagc cgattcgcgc ctgagttgtc
     9061 aggcggttgt cgccggcgaa gatttgattg tggaaatccc caaatacacc atcaaccacg
     9121 cgcgcgaaga acactgaaaa caggccgtct gaagccggca cgcttcagac ggcattgttg
     9181 cgcggataag gcgcaatcgc ccgaaaacag gcgttcgtac aggcggaact ttcgattcta
     9241 tagcgaatta acaaaaatca ggacaaggcg gcgagccgca gacagtacaa atagtacggc
     9301 aaggcgaggc aacgctgtac cggtttaaat ttaattcact atatattgat ttttatcggt
     9361 tttctgacgg aacggattgg tgcggcatcc gaggcggatt actcgggcgc gatgcacggg
     9421 tatttatcgg ttttgcagcc ggaaaaaccg ccggcgggtt actttcaggg cggcggtcgg
     9481 gcggtatgcc ggatagcgtt ccactttcgc ttctatattg attttgattg gttttctgac
     9541 ggaatgaccc gatgcggcat ccgggacggc ttgtgttttt tcctgcccgc ctgccggatt
     9601 ttcccatcct tgcgtgaaac cgaaagagac ggcggcgcgg cggacaagct cgagatagcg
     9661 tccttcaagc tccgggcagg cggcggacac gctttctgcc gtaaccgtga agccgccgcc
     9721 cgacccggaa aggcgttccg caatctgcgt gtagatacgc caacgttggg cgcgcaacat
     9781 tgccgcatcg caaccggcag ccgctttatc caaggcaatc aggccgcgcc ggttttggtg
     9841 gtgaaggcaa accgttactc tggagaggat atgttgcccg tccaatattt gatacagttt
     9901 gcgtatcagc agaatcaggc ggtcaaactc ctccatctcc gacagggaat caggatggtc
     9961 ggaagcggta taaaccagtt tggacaattt tgcggcaaac atattgttca tcaatcttcc
    10021 ttgtcggttg aaaccccgct cttcggggcg gtagaatcag atttgtttgg gagggacaac
    10081 tcctcccaga tcaggacgac acataggctg gtgcttgatg tgttgtccgg cgagttgaaa
    10141 cattcagcca tcttcaaggt gcggcagttt tgccgaaaca tattctacac ggcttcaatg
    10201 ccggacgata aaaggaaatt catatgaaat ggaccgacac ccggcgtatc gccgaagaac
    10261 tctatgacct gcacggcgaa accatcgatc ccagaaccgt gcgctttacc caactgcgcg
    10321 acctgattat ggcattgccc gaatttgatg acgatcccgc ccgttgcggc gaacgtattc
    10381 tcgaagccgt gcagcaggca tggatagacg aggcagaata agtttcggga atgccgtctg
    10441 aaatacggcg gtacgcggtt cgtgcttctg tttgcagtgg gaacggtttt accagtctcc
    10501 ttttttcagc ctatccagtt ggcggcggtc gcgcttggtc ggtctgccgt cgggatgggc
    10561 ggaagtgatg cggctgaatt ggtcgagctg tttgcgctct tccctcaatg ctgccgtttt
    10621 tgcgtcttct tcatacagaa gccgcgcctc ggatgccggg cggcgttggt ggttcaaacc
    10681 tttaaccttg attttatagg gaagggaatt gagcgtcagg tcgataatat cgccgatgtc
    10741 tatggtttta ctgtttttga ccttcgagcc gtttacttga accctaccca gttcgatgtg
    10801 cttttgcgca agggaacggg tcttgaaaaa acgtgccgcc caaagccatt tgtccagccg
    10861 catggcggaa gaatcgtgct tgtctttcat acgattttgt ttgaaataat tgaatttgtt
    10921 tcgagtttag cataagatac gccgcctata actagtatat atgcactaat ccactgtttt
    10981 ccatgctgtc cgaacacaaa aagagggtgt ggaaaagccg ttttggacaa taaattaact
    11041 gcggaatatg cacaaatagc gtatgatagc ggcagaatct gttgatgaga gcttcattct
    11101 atgaaacctg tttttttgga ttttgaacaa cccatagccg aactgaccaa caaaatcgat
    11161 gagctgcgtt tcgtccaaga cgagtctgcc gtcgatattt cggacgaaat acaccgtttg
    11221 cagaaaaaaa gcaacgacct gaccaaatcg attttcagca aacttacgcc cgcccaggtt
    11281 tcacaggttt cccggcatcc gcagcgtccc tatactttgg attacattga tgcgctgttt
    11341 actgattttg aagagctgca cggcgaccgc cattttgccg acgatcatgc gattgtcggc
    11401 ggattggcgc gtttcaacgg acaaagcgtg atggtcgtcg ggcatcagaa agggcgcgac
    11461 accaaagaaa aaatccgccg caacttcggt atgccgcgcc ccgagggcta ccgcaaagcc
    11521 ctgcgcctga tgaaaacggc tgagaaattc ggcttgcccg tcatgacctt tatcgatacg
    11581 ccgggcgcgt atcccggcat cggcgcggaa gaacgcgggc agtcggaagc catcggcaaa
    11641 aacctgtacg aactgacgcg cctgcacgtc cccgttttgt gtaccgtcat cggcgaaggg
    11701 ggctcgggcg gtgcgttggc ggtcgccgta ggcgattacg tcaatatgct gcaatactcg
    11761 acctattctg ttatctcccc cgaaggctgc gcttcgattt tgtggaaaac cgccgaaaag
    11821 gcggcggatg cggctcaggc tttgggcatt actgctgacc gcctgcaaaa gctggacttg
    11881 gtcgataccg tcatcaaaga accattgggc ggcgcgcatc gggatttcgg gcaaaccatg
    11941 aaaaacgtaa aagccgtttt ggaaaaacaa ctgcacgaag cgcaaagcat cccgcttgcc
    12001 gatttgcttt cgcgccgttt cgaccgcatt atggcttacg gcaaattttc ggaacaataa
    12061 ttcaggtaga acaagcagca agcagtttgt ctgaaactgc ttgctttttc tttatcggga
    12121 cggaaccgtg ctgactttag atgcgtttga gcaatgcttg aaggattgtt ttcctcaagg
    12181 tctgaatgga aaaaaaacag cagtggcatt aagcggcggc ttggattccg tcgttttgct
    12241 gcatctgctt gtccgcgccg gaaaaaaggg cggttttatt ccggatgcat tgcatatcca
    12301 tcacggcttg agtccccgtg ccgacgattg ggcagatttc tgccaaaact attgcgatat
    12361 gctcggggtg gggctggaaa cggttaaggt ctgcgtggaa aaaaacggtt tgggcatcga
    12421 ggcggcggcg cggcaaaagc gttatgccgc gtttgccgaa aaaggctttg acgttttggc
    12481 gttggcgcac cacagggacg atcaaatcga aacctttatg ctggcagtcg ctcgcggcgg
    12541 cggtttgcgc gctttggcgg ctatgcccac cgtccgccct tttggggaaa aaggcatcat
    12601 ctggcggcct ttgctgcctt tttcgcgcca agacatatgg gattatgccc aaaaacacgg
    12661 tttgccgaat atcgaggacg aaagcaatag cgatacggct tatttgcgaa accgcttccg
    12721 gcaccgtatt ttgcccgaac tttcggcgca gattccccat ttcgggcggc atgtactgaa
    12781 caatgtccgc gctttgcagg aagatttggc tttgttggac gaaatcatcg gtcaggactg
    12841 ccgttgggtc tgtgaggaag gcagtttcga tacggtgcgt tggcaggcgt tttccccacg
    12901 ccggaaaacc catattttgc ggtatttttt gaaggaaaac ggcatcccca tgccgaatca
    12961 gaatgccctt gccgacattg cccgggtttt gacggaggca aaaaccggac gttggaactt
    13021 gcaaggcttt gaattgcatc attatgcagg caggctgttt gtgttccgac tggaaaaaac
    13081 ggataaactg cggtttttga aagacaggca gataagcgga aatttaaggg aaatattgac
    13141 ggggcaggga tttgttttaa aacgtcatcc gtttgggctt cctgagcatc ttttggagca
    13201 ggacggaatt ttgaggacgg tagcggtatc ggatacgttg gctgtcggcg gcatccataa
    13261 ggatgtgaaa aaaatccttc aggggaaacg ggttttgcct gtcctgcgcc caatttggcc
    13321 gcttgttgcc gacagcggaa accgtccatt ggcgttggca aactgttgtg cggattttca
    13381 aatctcggtt tcagacggca ttttgcccgt ccatcctgac tttcccattt tattttgata
    13441 atatcgcaaa cagatttcgg cggcgttcag tcgggtattg tccggttgca tatttctaaa
    13501 aggcttgtga agtgagacac atcagttcga ccaataatga acacatcaga cacctgcacc
    13561 gcctgttgtc gcaaggaaag ttcagacggc aatacgccca aaccgttttg gagggcgtgc
    13621 acctgcttca ggttttcctg caatccggcg ggatgccggt cggggtatat attcccgagg
    13681 gcaaaatgcc gtctgaagaa atccgcaggc tgagggcggt tgtgccggaa ggcaggattt
    13741 tttccgtttc agacggcata ttgaaaaaaa tcagtagctt gagttgtgca gacgatatac
    13801 ttgcactgat tgatatccca ttgggcggaa ccttgccgga caaaggcgac tgtgtggttt
    13861 tggacggcgt gcaagatccg ggcaatgtcg gcaccgtctt acggagtgcg gcggcggcag
    13921 gggtaggtac ggtcgttttg ggcaggggtt gcgcggacgc gtggtcgccc aaggtattgc
    13981 gcgcggggat gggcgcgcat ttcttgtcgg gcatttatcc gcaggcggat ttggaaatat
    14041 ggttggcgcg ctataaaggc cgtgtgtttg ccaccgcctt gcgcgaggaa aagcaggcgg
    14101 ttttgtacgg cgaagatttg tgcggaccga cagcctgggt gtttggcaac gaaggcgcgg
    14161 gggtcggtaa agcggttttg gacagggcgg acaagtgtgt caggattccg atgcacgatg
    14221 caaccgagtc tttaaatgtc gcgatggcgg cgacaatctg cctgtttgaa cagatgcggc
    14281 agcggtcggc gtattgagga aaagaaatgc cgtctgaaaa aatctattac ggcgtattga
    14341 ttttcttatg tatcgcttct atgctgctgt cgccgttttt ttatgcgggt gctttgaagc
    14401 ccaagaaggc ggcattgcgg aaggacgggc agtggaaact catcttattg tccaataccg
    14461 tggcggcggc ggttttggct tgggtgtggt ggaaatggtt ttgacagata ttgctcaaaa
    14521 tcgtgctaat ggaatccgaa caaataaaga atttggtaaa aaatttgtta aatcaacgaa
    14581 ttaaagtttt gtggaaaaca aaacagctct aagcaaatag ggcgtttgtc ggtaaatacg
    14641 gaagagttgc ggcattatcg ggcatcttta acaagtagtg ccgtcttggc aggcaatcgg
    14701 tttttatggg cagcttgcaa aatcgcggat ataaaattgc gaatcggtta aagtgtgggg
    14761 acgctatgaa aaattgcgaa tttttttatg acccgacaag ggcaatctat gatagcggtg
    14821 cagattactt aactagggaa aaacatagat tagtcgtaat tgcaaatagt gcttgggggc
    14881 tattacttaa tttatcttgt tattatgacg aggttttgga aaagcggaaa ataccgttcg
    14941 gcaaacagga aattgatgac gatatggaca aagtgtccgc ccttaagcgg aagtttaaag
    15001 atatttctga aatcaaagta ggggatggtt gggaataccc gttcaattat gagcagggaa
    15061 tgaaagaatt agatgaagtg ctattgaaat acattccctt ttttgaagaa gaacgataaa
    15121 ggaggttgat atgcgcgtat ctaaaataat tggaagtatg ttgcttgtta cagcggttca
    15181 gaccgtattt tcggcaaatg tttacgcgtg ccgccataat ggtaaaacca gttacagcca
    15241 aactccggga aaacattgta ccaacgcggg tttggggcgg gaccgggtgt acagttcggt
    15301 tagaccggca gtaaaagaca gggcggaaga cgcaggggtc ggcgattatt cggacacggt
    15361 gagggacgaa cacgtccaaa atccgaaagg gaatgcacag aaagacggtt cggatgccgg
    15421 catcaggccg cattgattga aaccgaatca gccatgacgg cgttgaataa tagaatttga
    15481 acgattaggg aactctgatg aaacacatcc acattatcgg tatcggcggc acgtttatgg
    15541 gcgggattgc cgccattgcc aaagaagcgg ggtttaaagt cagcggttgc gacgcgaaga
    15601 tgtatccgcc gatgagcacc cagctcgaag ccttgggcat aggcgtacac gaaggcttcg
    15661 acgcggcgca gttggaggaa tttaaagccg acgtttacgt catcggcaat gtcgccaagc
    15721 gcgggatgga tgtggttgag gcgattttga accgcgggct gccttatatt tccggtccgc
    15781 aatggctgtc ggaaaacgtg ctgcgccatc attgggtact cggtgtggcg gggacgcacg
    15841 gcaaaacgac caccgcgtcc atgctcgcat gggttttgga atatgccgga ctcgcgccgg
    15901 gcttccttat cggcggcgta ccggaaaact ttagcgtttc cgcccgcctg ccgcaaacgc
    15961 cgcgtcaaga cccgaacagc aaatcgccgt ttttcgtcat cgaagccgac gaatacgaca
    16021 ccgccttttt cgacaaacgc tccaaattcg tgcattaccg tccacgtacc gccgtgttga
    16081 acaatctgga attcgaccat gccgacatct ttgccgattt gggcgcgata cagacccagt
    16141 tccatcacct cgtgcgcacc gtgccgtctg aaggcttaat catctgtaac ggacagcagc
    16201 aaagcttgca ggatacttta gacaaaggct gctggacgcc ggtggagaaa ttcggcacgg
    16261 aacacggctg gcaggccggc gaagccaatg ccgacggctc gttcgacgtg ttactagacg
    16321 gcaaaaccgc cggacgcgtc aaatgggatt tgatgggcag gcacaaccgc atgaacgcgc
    16381 tcgccgtcat tgccgccgcg cgtcatgtcg gtgtcgatat tcagaccgcc tgcgaagcct
    16441 tgggcgcgtt taaaaacgtc aaacgccgca tggaaatcaa aggcacggca aacggcatta
    16501 ccgtttacga cgactttgcc caccatccga ccgccatcga aaccacgatt caaggtttgc
    16561 gccaacgtat cggcggcgcg cgtatcctcg ccgtcctcga gccgcgttcc aacacgatga
    16621 agctgggcac gatgaagtcc gccctgcccg aaagcctcaa agaagccgac caagtgttct
    16681 gctacgccgg tggcgtggac tggaacgtcg ccgaagccct cgcgcctttg ggcggcaggc
    16741 tgcacgtcgg caaagacttc gatgccttcg ttgccgaaat cgtgaaaaac gccgaagcag
    16801 gcgaccatat tttggtgatg agcaacggcg gtttcggcgg aatacacacc aaactgctgg
    16861 acgctttgag atagcccggg cgatgccgtc tgaaagccct tcagacggca tcgcccggct
    16921 gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt caaacgcgtt acacttgccg
    16981 ccgctgtttt cagccatttg attacccgca accgccgtca ttgcgccggc ggtttgcctg
    17041 tcagcgtcat tgcgccgctg taaatacgaa agaacacatt atgaccgtat cccccgtcgc
    17101 cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg cgctattgga aaaaatgcga
    17161 tgtcgaagcc ctgttcggac ttcccttcct cgacctcatt taccaagccg ccgaaatcca
    17221 ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg ctgttgtcca tcaaaaccgg
    17281 cggttgtccc gaagactgcg cctattgtcc gcaatcggcg caccacaata ccaatctggg
    17341 caaagagcag atgatggatg tggatgaaat cgtcgaaaaa gccaaaatcg ccaaatcgcg
    17401 cggcgcaagc cggttttgta tgggcgcggc gtggcgcggc cctaaaccca aagacgtgga
    17461 gacggtttcc gcaatcatca aagccgtcaa gggcttgggt atggaaacct gcggcacgtt
    17521 cggtatgctc gaagaaggta tggcggaaga cttgaaagag gcgggcttgg attattacaa
    17581 ccacaacctc gacaccgacc ccgaccgcta caacgacatc atccacaccc gccaacacga
    17641 agaccgaatg gacaccttgg gcaaagtccg caacgccggt ttgaaagtct gctgcggcgg
    17701 catcgtcggg atgaacgaaa cccgcgccga acgtgccggg ctgattgcca gtctcgccaa
    17761 tctcgacccg cagcccgaaa gcgtgccgat taaccggttg gtcaaagtgg aaggcacgcc
    17821 gcttgccgat gccgaagatt tggactggac ggaatttgtc cgcaccatcg ccgtggcgcg
    17881 gattacgatg ccgcaaagtt atgtccggct gtcggcaggg cgcagcaata tgcctgaagc
    17941 aatgcaggcg atgtgcttta tggcgggcgc gaactcgatt ttttacggcg acaagctgct
    18001 gaccacgggc aatcctgatg aggacggcga cagaatcctg atggaaaagc tcaacctgta
    18061 tcccttgcag tttgaaccgg aaggcgaggt cgccgaagtg gaaaaagcct ctgggattaa
    18121 agtggattat tgacgattga aaaatgccgt ctgaaacccg gaaaaaggct ttcagacggc
    18181 atttgtccgg acggcatttc caatatcttt ttaccggcgc gtgatgctgc cgtcgggcga
    18241 gacatccagc ccgttcccct tggggaaatc gctgcggttc agataaataa tccgcccgat
    18301 aacggttttc tcggggtcga ttttggggat tttatcgtcg gcttgggtaa tagggatgat
    18361 agttccggaa ataaatttac cctgtttgtc ggtgataatt ttgaaaatcg gcgcaatgcc
    18421 gctgatgccg gaggtgttga ttgcgccgta ggtggcaaag ttgccgccgc tgtaggagat
    18481 gaagcggtcg cggtaaagtt cgacggcgcg tgtaacgtgc ggcccctgcc cgaatacgac
    18541 atccgcgccg gaatcgacgg caagccgtgc aaactcgacg acgttgcccc tgttttcccc
    18601 atagaagatt tcggtatcga acggcaggtg ttccgcctgt ttcccttccg cgccgccgtg
    18661 gaacatcaca ataacgatct cggctttttg tttggttttg gcaatcagtt ttttgacttt
    18721 ggcatagtcg ttcagtttga cggcggcaag gttgggggcg aaggagacga agccggatct
    18781 tacgccgttt ttcttcagga tggcggtttc aaacctgttt tcgatgcccg aatatttgat
    18841 gttcaattcg tcaaggttcg ccctttatgc cgtttccgca ccgcaaaccg ccggggtcaa
    18901 gccctcggcg catcctgccg gaacggaatc cccgtgctgc cgattgacgg ttcaaacccc
    18961 gcgcccgttt caaataccgg cgatgtggac ggacaggatg cgcctgacga aaagacagcc
    19021 gataccgttt ccattatcgg cgtgggcgac attatgctcg gcagcaatta tccggtcgat
    19081 tacctgcccg ataccaatat tctgaaaaac gtcgaatctg ccttgcaaga cgcggacatt
    19141 accgtcggca acctcgaagg cacgctgttt gacgaaggcg gtacgccgaa aaaatgtgca
    19201 accccccaaa atatgctatg cattccgaac gccctccgca tacgggcaat accttgccga
    19261 cgcgggattc gactacctca gcttcgccaa caaccacagc aacgacttcg gcgcgcaagg
    19321 catcacggca acggcggcga gcggcagctc ttttacatac tcgatcgcgc taaagccgct
    19381 gccgataacg aggccaaaat tgcggaaaat accgccatcg cccagataaa tttgtccatc
    19441 atcagacctt tactgttcag acgagacagc atttgccgca cgttttgggg cttatctttc
    19501 gatttgcgct acgtcgcgca ccgcgccttt gtcggcggaa gtcgccatcg cgccgtaagc
    19561 tcttaatgcg gcggagacgt agcggtcgcg gttttccggt ttccacgctt tgctgccgcg
    19621 tgcttccatt tcggcacggc gggcggcgag ttcttcgtcg gaaatgacaa ggcggatgct
    19681 gcggttgggg atgtcgattt cgacggtatc gccttcgtgt accaaaccga tcgcgccacc
    19741 ttccgccgct tcgggcgagg cgtgtccgat ggacaaacct gatgtgccgc cggagaagcg
    19801 tccgtcggtt aagagagcgc aggctttgcc gaggccttta gatttcaggt aggaagtcgg
    19861 atacagcatt tcctgcatgc ccgggccgcc tttcgggcct tcgtagcgga tgatgacgat
    19921 gtcgccggcg acgatttggt tgcccaaaat gccttctact gcgtcttctt ggctttcaaa
    19981 cacgcgggcg cggccggtga atttgaggat gctctcgtcc acgcctgcgg tttttaccac
    20041 gcagccgcgc tcggcgatgt tgccgaacaa gaccgccaaa ccgccgtctt gcgagtaggc
    20101 gtgtgccacg tcgcggatac agcctttttc gcggtcgagg tcgagggttt tccacatacg
    20161 gttttgcgag aacgcttggg tggtgcgtac gccgccgggc gcggctttga agcgttcgat
    20221 ggcgtgggtg ttttcagggt tggttacgtc ccatttttca atcgcgtctt tcagcgtcgg
    20281 cgcgtggata gtgtgcacgt cggtgtgcag cttgcccgct ttgtccaatt ctttcaggat
    20341 ggcgaagatg ccgccggctc ggtgcacgtc ttccatatag tagtcgtggt tgttgggtgc
    20401 ggttttgcag atgcagggca caacgcggct taagcggtcg atgtctgcca ttttgaaatc
    20461 cacgcccgct tcgttggcaa cggcgagtaa atgcaaaata gtgttggtgc tgccgcccat
    20521 ggcaatgtcc atggtcatag cgttttcaaa cgcttttttg gtggcaatgc tgcgcggtaa
    20581 cacggtttca tcgttttgct cgtaatagcg tttggtgatt tcgacaatca tacggccggc
    20641 ttcgaggaac agttctttgc ggccgacgtg ggtggcgagg taagaaccgt tgcccggcag
    20701 ggacagcccg agtgcttcgg tcaggcagtt catcgagttt gccgtaaaca tacccgaaca
    20761 cgagccgcag gtcgggcagg cgttttgttc gacttcttca acttgccggt tgctgatatt
    20821 gtcgtccgcc gattcaatca tcgcgtcaat caagtccaaa cggcgttcgg gctggatgtt
    20881 tgccacgccg ataactttac ctgcttccat cggaccgccg gaaacgaaga tggtcgggat
    20941 gttcaggcgc atcgcggcaa tcagcatgcc cggggtgatt ttgtcgcagt tggaaatgca
    21001 caccagcgcg tcggcgcagt gggcattgac catatattcg atggagtcgg caatcaaatc
    21061 gcggctgggc agggaataca gcataccgct gtgtcccata gcgatgccgt cgtcgatggc
    21121 gatggtgttg aattctttgg cgatggcgcc ggctttttcg atttcgcggg caaccagctg
    21181 gcccatattg tgcaggtgga catgacctgg cacgaattgg gtgaacgagt tggcaacggc
    21241 gataatcggt ttgccgaagt cggtttccat cacgccggtg gcgcgccaca acgcgcgcgc
    21301 gcccgccata ttgcgaccgt gggtggaggt tttggagcgg tattctggca tagtgtgttt
    21361 ccttgtgcct ataccgtctg aaagacaggg ctgtttcaga cggtatcggg tacggttttt
    21421 tagagtggga aaagagggta ttttatacca agtatcggaa ttttgcggga ttgaaacggc
    21481 gtgcggcaaa aaagaaaatc cccgcaggaa tgcggggacg ggttcaggcg cgggcaatcg
    21541 cgacggcttt ggatgcgtcc caaaaatcaa cgggtgcatt taatacgggt ttgacgatgc
    21601 ccgtccgtat ggcgaaatcg ccgaaacctt cgccgatatt gcgttctgcc gcccatttgc
    21661 cgatcaggtc gtccaattcg gcaaggattt cgggcagggt gatgttttct ttgtaaagac
    21721 gggggatgcg tacgccttca cggtcgccgc cgatatggag gttgtagcgt ccgacggctt
    21781 tgccgaccag tccgatttcc gccaacatcg cccgtccgca gccgttcggg cagccggtaa
    21841 tgcgggtaac gatgtagtcg tccgacgtgc cgtgtttcgc cataatctta tccagctcgc
    21901 cgatgaagtc cggcagcacg cgttcggctt ccgccattgc cagcgggcag gtcgggaagg
    21961 aaacgcacga catcgcattt tcgcgcagtt tgctgacacc gtcgtgtatc agcccgtatg
    22021 agcgggcgag ctgttcgatc cgggctttgc ctccttcggg tacatttgcc acgatgaggt
    22081 tttggttggc ggtgatgcgg aagtcgcctt tgtggatttt ggcgatttcc aacacgccgg
    22141 tcagaagctg tttcccgcct tcgtcaacca aacgcccgct ttcgatgaaa agggtcaaat
    22201 gccagttgcc gtctatgcct ttcacccagc cgatgcggtc gccgcgcccg gtaaatttga
    22261 acgggcgtat gggttcgaac ggcataccca tacggcgttc gacttccgcg cggaagttgt
    22321 ccaagcccat attttgaatg gtgtagcggg tgcgggcgtt tttgcggtcg ctgcggttgc
    22381 cgaagtcgcg ctgtgtggtt accaccgctt cggcggcctt cagcgcgtgt tccggaggca
    22441 cgaaacccag ttccagtgaa atgttcggat aggttttggt gttgccgtgt tccatcgaaa
    22501 gcccgccgcc cgccaaaaca ttgaatccgg caagctgtcc gttgccgtct gaaatggcga
    22561 caaaatccaa gtcgttgccg tagcagtcca catcatttaa gggcgggatg acgactgcgg
    22621 ttttgaattt gcgcggcaga taggttttgc ccaaaatcgg ctcgtcttct tgaaggaagt
    22681 cgtcggaact ttgaactttt ttgccgtcca cccacacatc cagataaccg cgcgtgcgcg
    22741 gcagcaggtg ttcggaaatc tttttcgcgt attcgtaagc ctgccggtgc agttcggact
    22801 cgatcgggtt ggacgtgcaa agcacgttgc ggttcatatc cgccgccgtg gcgatggaat
    22861 ccaaacccag tttgtgcaag aggcggtgca tcgtctgcaa cttggctttc ggcacgccgt
    22921 gaaattggaa ggtttgccgg ttggtcagcc ggatggagcg gtaatgactg tttttccggg
    22981 caaatttgtc cagttctatc cattgggacg gtttgatgat cccgcccggc agccggcagc
    23041 gcaaaagcat aaatttcaag ggctcgagtt ttgcctcggc gcgttcggcg cggatgtcgc
    23101 ggtcgtcctg ctcatacata ccgtggaagc ggatgagttg gaagttgtcg cctttgaagc
    23161 cgcccgtgag cgggtctttc aaatcgtcca aaatcgtgcc gcgtaaaaaa ttgctttcgg
    23221 ttttcagacg ttcgttgtcg gatagcggtt tttcttgcca cgccaaacct tttgtcttgg
    23281 tctgtacggt cattttgtgt tcctcccgat tatatttaat caataaacat cacgctgata
    23341 gcgtttttct tcgcgcaaca tatccaaata ttcttctgcg ccctcttcgt ccaaatgtcc
    23401 tgccccgata atcacatcca gcaaggcggc ttccacgtct tttgccattt ttgccgcatc
    23461 gccgcacaca tagatatgcg cgccttcctg cagccattgc caaagtcctt ccgcctgttc
    23521 gcggattttg tcctgcacat agattttttc ttcctgatcg cgcgaccagg cgaaatcgta
    23581 tctgtgcagg aagccgtctt tggcaaactg ctgccattcg gtctgataga ggaagtcggc
    23641 ggcaaaatgc ggattgccga aaaacagcca gtttttgcct gttgcatttt ctgcggcgcg
    23701 ttgttgcaaa aaagcgcgaa acggtgcaat gcccgtaccg gagcctatca ttacaatggg
    23761 tttgcttttg tcttcgggca gtctgaagcc gtcgttgcgc tccacaaata cacgcaccgt
    23821 gccgtcttct tcgacgcaat cggcaagaaa accggatgcg ccgcccgttc tggcgcggcc
    23881 ttcgtgttca aaacgaacca cgccgacggt caggtgcact tcgtccccca cttccgcctg
    23941 cgacgaggaa atcgaataca ggcgcggcgc tagcgggcgc agcagaccgg cgaattgttc
    24001 cgccgtcagt tttgccggga agcggtgcag cacatcggca atcggcgtgc tttgcacaaa
    24061 gccttgcaaa acggcgttgt cggcagcaat acggtcgagt tcgtcatcat cggcgaacgg
    24121 ggcatagcct ttgacaaagg caggggtgtt ttgcgtgagt tcaaaatggg ataacagtgc
    24181 ggaggcaacc ggcagggttt ttccgcccgc ctgtatttcc gttgccggat cgatgccgag
    24241 caggtctagg atttccctga ccagtgccgg atcgttgtca aaccaaacgc cgagcgcgtc
    24301 gcccgggagg tagtgcaaat ccgaaccgct caaatcaagt tcgatgtggc gcacgtcttt
    24361 atcggattgg cgggcggtga ttttctgatt ggccagcagg gcggcgggaa aggggtctgc
    24421 cttgcagtac ctgccctccg gtaccgtctg aaggtcggcg gggggcattg cctgtggcgc
    24481 gggtgttgcc cggttttttg cggcttcttc ttttaagagt gcggcgatat tgtctgtcca
    24541 ggcgtttgca gaggcggtaa agtccaaatc cgcatcaacg cgttcgagca ggcgtttcgc
    24601 ccccaattct tcaaaacgcc ggtcgaaatc tttacctgcc tgacagaaat tcggatagga
    24661 gctgtcgccc aagcccagta cggcaaattg gagtttgtcc aatttcgggg cttttttgcc
    24721 gttcagcagt ttgtgcagca cgacggcttc ttccggcggt tcgccttcgc cctgggtgga
    24781 cgtaaccagc agcaggcggc gttcgtctgc gatgtttttc gccttgtagt ctttcagttc
    24841 ggcgcgcctg acttgtatgc cggcggcttt caggctgtcc gccgctttgt cggcaacgga
    24901 tttcgcattg ccggtttgcg aggcggaaag gacggtaacg gaaaagggtt ctgccgccgg
    24961 caatgctgtc tgaagcgcgg gcagccctgc ggatgccccg tttcctgctt ttgcccaagc
    25021 atagccggac agccacgccc attgtgccgc gtccagcccc gacaggagct gcgtgatttc
    25081 gggcggcata ggcggtaatg gcggatttat gttctgcata tcgtgttcac tcataaaatc
    25141 atacctgccg caacagtgcc gtatgtcgct tcgtctatca ggataaacga accggcggcg
    25201 gtattttccg cataaggcgt tgccgtaacg ggtttttgaa ggttgatgcg gactttggcg
    25261 atgtcgttca tcttcaagga ttccgcgccg gcctcttgtt ccagcgtgcg gacatccaaa
    25321 acgctttcaa tttccccgac ttttgccggc agggtttgcg tgccgtgctt gagcaggtat
    25381 ctgcgcgcgg tattgagcgg acgttcgtca aaccagcaaa gcgtggcttc cagatgtttt
    25441 tgcggggcga gcggggaatt tttatcgaca aaaaggtcgc cgcgcgaaac atcgatgtcg
    25501 cgatccagcc ggagggttgc cgcctcgccg gcaaaagcct gcgccacttc gcctttgggt
    25561 gtgatgattt cagacacttt ggcggtcagc ccgttcggtt cgatgcggac ggtttgttcg
    25621 acggcgaccg aaccgcgttc gatgcgcccc tgatagcctc ggaaatcatc ggccttgtcg
    25681 gcatcttggc ggacgaccag ttgcacgggg aaataaaaat cgtcggcggt gcggctgact
    25741 tcgtcctccc ccggcaggtt ttccaaaatg gacaataagg gttcgccttt ataccaaggc
    25801 atattgccgc cggggtaaac gatgttgtcg cccaagagtg cggacaccgg tacgaaatgc
    25861 acgtctttca aaccgagctg ttcggcaagt cggcggtatg cctccacaat ggcgttgaat
    25921 ttgtcttcgc tgtaatccag cagatccatt ttgttgaccg ccaccacaat atgcgggcag
    25981 ttgagttggc ggaggatggc ggaatggcgt ttggtctgcg gcagaagctg caagggctgc
    26041 gcgccgaaat ccagttggga tgcgtcaacc agtacgactg ccgccgaagc ggtgcttgcg
    26101 cccgtaacca tattgcgcgt gtattgttcg tgccccggcg tgtcggcgat gatgaatttc
    26161 cgtttcgccg tggaaaaata gcggtatgcc acatcgatcg taatgccctg ttcgcgttcg
    26221 gcttccagtc cgtcggtcag gatggagaag tctatggctt ctttcaaacc tttgcttttg
    26281 ccggattcca aggttttgat ttggtcggac agcagggctt tgctgtcgta gagcagtcgt
    26341 ccgattaagg tgcttttgcc gtcatcgacg ctgccggcgg taatgaagcg gagcggggtc
    26401 tggtgttgtg ccgtcatatt ttcttcctca tatctgctta aagggttctt gaaatttaga
    26461 aatagccttc ttttttgcgt ttttccattg ccgcctcgct tgcctgatcg tccagccggg
    26521 tcgcgctgcg ttcggaaatg tcggcaaccg ctgtttctct gataatctcc gtcggcgtgg
    26581 acgcggtgct ttctaccggg caggtgcagc tgatgtcgcc gacggtgcgg aagcggacat
    26641 caaggatttc ggaggtttca gacggcattt tcggggtgag cagcgttacg gggaccagca
    26701 gccccctgcg tctgaccact tcgcgcctgt ggctgtaata aatcggcggc agctcgaggt
    26761 tttcgcgggc gatgtattgc caaatgtcga gttccgtcca gttggaaatc gggaagacgc
    26821 gcatattttc gcctttgtgc agcctggtgt tgtacagcga ccacagctcg gggcgttgcg
    26881 ccttcggatc ccattgtccg aactcgtcgc ggaacgagaa aatccgttct ttggcgcggg
    26941 ctttttcttc gtcgcgccgc gcgccgccca taagcgcgtc aaaaccgttt gcctcgatgg
    27001 tttccaacag cgtaaccgcc tgtgccgcat tgcgcgaatc ggtttctttg cgtaagacca
    27061 ccgtgccttt ggcaatggag tcttccacgc gccccactat caggcgggca ttgagttttg
    27121 ccgcctgcgc gtcgcggaag gcaatcactt cggggtagtt gtgccccgtg tcgatatgca
    27181 ccagcgggaa gggcagtttt accggcctgc tgcccagccg gaaggctttg caggcgaggg
    27241 cgagcaggac cacggaatct ttgccgccgg aaaagagcag ggcggggttt tcgcattctg
    27301 ccgccacttc gcggatgatg tggatggatt cggattccaa ccagtcgagt tgggcgttgt
    27361 tcggttcggt tttcgtcata ccatattcct tatttcttct gtctgatatt tatgaattat
    27421 ttgtgcagcc cgcattcttt gctgtttctg ccttcccacc accaccgccc ggcgcggatg
    27481 tcttcgcccg ccttgacggg gcgggtgcag gggtcgcagc ctatgctggg aaatccttgc
    27541 cggtacaaat cgttgtaagg cacattgttg gcgaggatgt atgcccacac gtcgtgttcc
    27601 gaccagtcga aaatcgggtt gtatttgccg atgccccgtc cggcatcgta ttcggcaaac
    27661 ggcagttccg tgcgtgtggc ggattgttcg cggcgttgcc cggtaagcca ggcgtccgcg
    27721 cctgcaatgg cgcggttgag cggttcgatt tttcggatgc ggcagcattc gcggcgcgct
    27781 tcaacgctgt cgtaaaaggc aaacctgcct ttgctttcca cataacggtc ggcatcttct
    27841 cgaaccggcc ggaaacgctt tatccgcaaa tggggatatg cgcgtccgag cctgtccagc
    27901 aggttcaggg tttccgtgtg gagcagcccc gtatccaagg taaaaatgcc gatattgagg
    27961 ttttcgccgg cgataaggtc ggtaatcacc atatcttctg ccgcaaggct gctggcaaac
    28021 cgtgcatccc ggtggctgcc gacaatccgg tgcaggcgtt gtttgagggt ttccgttttt
    28081 tccgtaaggg cggtttcgcc gccggatccg atatgcggta tctgccacag ggcgggtttg
    28141 aacagtgtcg tttccatttt tcccgcctta tgccgcccgt tgtccggcat tcagtccgcc
    28201 caatgcggga tacgcctgcc cgacccggtt ttctccttcg ccgttttcac cgaaccaggc
    28261 gagtttttcg tgcagcccca ccacttcgcc tatgacaatc aatgccggat tcggcgcggt
    28321 ttcggcgagt tcggcaaggt tggcgagcgt gccggttgcg gttttttgag ccggcagcgt
    28381 gccttggctg ataacggctg ccggcgtgtc gggcgagcgt ccgtgctgtt gcagccgttc
    28441 ggcaatcagg gcggctttga gcgcacccat ataaatcacc aaggtctggc ggctgcgggc
    28501 gagggtctgc cattcgatgt cgggcgcatc cgccttgcgg tggccggtta cgaaaaccgc
    28561 actttgggca taatcgcggt gcgtgagcgg gatgccggca taggcggtcg cgccgacggc
    28621 ggcggtaatg ccggggacga ccgaaaacgg aatctgatgg cgtgccaagg tttccaattc
    28681 ttcgccgccg cgtccgaaca cgaaagggtc tccgcctttc aaacgcacca cgcgcctgcc
    28741 ttcgcgggcc agcctgacca taagcgcatt ggtgtcctct tgcggggtgc gctcgccccg
    28801 ggcgcgcttg ccgacaaaaa tccgttccgc atcgcggcgg acgagggaca gtatgccgtc
    28861 tgaaaccagc gcgtcgtaaa gcaccacgtc tgcctgctgg atttcctgca gccctttgag
    28921 cgtcagcagc cccgcatcgc cgggacccgc gccgaccagc gagacggagc cgccttgatc
    28981 attttgacga ctttgttcca attggcctgc caattcccgt tcggcaaggg tgttttgccg
    29041 gtttttggcg agggtggcga aacgtccgtt aaactgcttt tcccaaaagc ggcggcgttc
    29101 ggtaacggat ttcagtttgc ccttgacggc atcgcgccac cttcctgaaa tttccgccat
    29161 atcgcccaaa gacggcggca gcagggcttc cagcctttca cgcagcagtc gggcgaggac
    29221 gggcgcgctg ccggagctgg aaacggcaat ctgaaccggg ttgcggtcga taaccgacgg
    29281 gaagatgaag ctgcaatggt cgcggtcgtc caccacgttg accggctttt ggcagctttc
    29341 ggcaagatgg aaaacgcgcc ggttgagggc ttggtcgctg cttgccgcaa tgatgaggaa
    29401 aaccgtgcgg atgtgttcgg cacgaaattc ttcggcaagc cacaggattt tgttttccgc
    29461 cgccaacgcg gagagttcgg cattcaggtg ttttgcggca accctgacct ctgcgcccgc
    29521 cttcagcagc aggctgattt tgcgtgcggc gaccgcgccg ccgcctacga ccaatacggg
    29581 gcggccggcg aggttggcga aaatagggaa ataattcact ggctgactcc tttgctgttt
    29641 gcccgcacct tgtttccgat acggtgcgtc gcggcatttt tgtcggaatg cgggtcattt
    29701 tagacaaaag gattttcccg gttaaataat aaaaaggcat ttgttagaag ctgaaagcta
    29761 tatgagggcg gctgcggatg cggcggttt
//
LOCUS       DAWKOD010000007        29410 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000007 DAWKOD010000000
VERSION     DAWKOD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.007"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(76..246)
                     /locus_tag="WJK73_000213"
     CDS             complement(76..246)
                     /locus_tag="WJK73_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ7556757.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            259..477
                     /locus_tag="WJK73_000214"
     CDS             259..477
                     /locus_tag="WJK73_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556758.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            474..815
                     /locus_tag="WJK73_000215"
     CDS             474..815
                     /locus_tag="WJK73_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556759.1"
                     /translation="MTYRELVERQLAVRQAGLQIGLQKANEQEPFIAAVAETLSRTMW
                     GYVMRMDARFEVTFIVDLGHVAFEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGI
                     AACRVVFGDVL"
     gene            812..1093
                     /locus_tag="WJK73_000216"
     CDS             812..1093
                     /locus_tag="WJK73_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556760.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            1093..1308
                     /locus_tag="WJK73_000217"
     CDS             1093..1308
                     /locus_tag="WJK73_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556761.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            1311..1817
                     /locus_tag="WJK73_000218"
     CDS             1311..1817
                     /locus_tag="WJK73_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7556762.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1814..2008)
                     /locus_tag="WJK73_000219"
     CDS             complement(1814..2008)
                     /locus_tag="WJK73_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556763.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            2020..3639
                     /gene="terL"
                     /locus_tag="WJK73_000220"
     CDS             2020..3639
                     /gene="terL"
                     /locus_tag="WJK73_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7556764.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3718..5277
                     /locus_tag="WJK73_000221"
     CDS             3718..5277
                     /locus_tag="WJK73_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7556765.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLQAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            5270..6616
                     /locus_tag="WJK73_000222"
     CDS             5270..6616
                     /locus_tag="WJK73_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ7556766.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALALPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6720..7217
                     /locus_tag="WJK73_000223"
     CDS             6720..7217
                     /locus_tag="WJK73_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7556767.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            7426..8490
                     /locus_tag="WJK73_000224"
     CDS             7426..8490
                     /locus_tag="WJK73_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7556768.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            8512..9414
                     /locus_tag="WJK73_000225"
     CDS             8512..9414
                     /locus_tag="WJK73_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7556769.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            9452..9925
                     /locus_tag="WJK73_000226"
     CDS             9452..9925
                     /locus_tag="WJK73_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556770.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9922..10347
                     /locus_tag="WJK73_000227"
     CDS             9922..10347
                     /locus_tag="WJK73_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7556771.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            10331..10999
                     /locus_tag="WJK73_000228"
     CDS             10331..10999
                     /locus_tag="WJK73_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7556772.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10996..11184
                     /locus_tag="WJK73_000229"
     CDS             10996..11184
                     /locus_tag="WJK73_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7556773.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            11184..12611
                     /locus_tag="WJK73_000230"
     CDS             11184..12611
                     /locus_tag="WJK73_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7556774.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELAAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12625..12999
                     /locus_tag="WJK73_000231"
     CDS             12625..12999
                     /locus_tag="WJK73_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7556775.1"
                     /translation="MSTEYVGSVILYLGANEVEVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            13175..13561
                     /locus_tag="WJK73_000232"
     CDS             13175..13561
                     /locus_tag="WJK73_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7556776.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13548..14012
                     /locus_tag="WJK73_000233"
     CDS             13548..14012
                     /locus_tag="WJK73_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ7556777.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            14194..14373
                     /locus_tag="WJK73_000234"
     CDS             14194..14373
                     /locus_tag="WJK73_000234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556778.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            14502..14891
                     /locus_tag="WJK73_000235"
     CDS             14502..14891
                     /locus_tag="WJK73_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556779.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPIVTDTPS"
     gene            14849..15097
                     /locus_tag="WJK73_000236"
     CDS             14849..15097
                     /locus_tag="WJK73_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556780.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            15094..17143
                     /locus_tag="WJK73_000237"
                     /pseudo
     CDS             15094..17143
                     /locus_tag="WJK73_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            17143..18510
                     /locus_tag="WJK73_000238"
     CDS             17143..18510
                     /locus_tag="WJK73_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7556781.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            18494..19633
                     /locus_tag="WJK73_000239"
     CDS             18494..19633
                     /locus_tag="WJK73_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7556782.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19634..20263
                     /locus_tag="WJK73_000240"
     CDS             19634..20263
                     /locus_tag="WJK73_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ7556783.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            20324..20677
                     /locus_tag="WJK73_000241"
     CDS             20324..20677
                     /locus_tag="WJK73_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ7556784.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20674..21159
                     /locus_tag="WJK73_000242"
     CDS             20674..21159
                     /locus_tag="WJK73_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7556785.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            21162..21737
                     /locus_tag="WJK73_000243"
     CDS             21162..21737
                     /locus_tag="WJK73_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7556786.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21734..22300
                     /locus_tag="WJK73_000244"
     CDS             21734..22300
                     /locus_tag="WJK73_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7556787.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            22303..23538
                     /locus_tag="WJK73_000245"
     CDS             22303..23538
                     /locus_tag="WJK73_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556788.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVV"
     gene            23701..24585
                     /locus_tag="WJK73_000246"
     CDS             23701..24585
                     /locus_tag="WJK73_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ7556789.1"
                     /translation="MNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIGAN
                     AMNKALTAGYSTIIGADAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLASSE
                     KCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAVNI
                     RSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREWWA
                     NPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKAIQ
                     EMAARIETLETEMKESKK"
     gene            24582..25205
                     /locus_tag="WJK73_000247"
     CDS             24582..25205
                     /locus_tag="WJK73_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556790.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            25206..25694
                     /locus_tag="WJK73_000248"
     CDS             25206..25694
                     /locus_tag="WJK73_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556791.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            26042..26881
                     /locus_tag="WJK73_000249"
     CDS             26042..26881
                     /locus_tag="WJK73_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ7556792.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            27141..27674
                     /locus_tag="WJK73_000250"
     CDS             27141..27674
                     /locus_tag="WJK73_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ7556793.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27737..28921)
                     /locus_tag="WJK73_000251"
     CDS             complement(27737..28921)
                     /locus_tag="WJK73_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ7556794.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSFHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(28961..29365)
                     /locus_tag="WJK73_000252"
     CDS             complement(28961..29365)
                     /locus_tag="WJK73_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ7556795.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7509 a         7633 c         8233 g         6035 t
ORIGIN      
        1 gggcaatcac tcccgcaaag tcggaaactg acaaaaaagc ccgcgtaggg cgcgggctga
       61 gggtgaaagc ggattttata cctcttttac aggggtagcg gcggtagtgc ttttcagcaa
      121 atcgactgcg tgctggcagt tttgcttgct ggtgtagcct tcgccctgag cgatgatttc
      181 atggttggct gctttcaaac gccaacggta ttcgcctttt gcgtctttat agatttcaaa
      241 atacataagg tttctcctat gaatgagtac acgttttctt accgctttaa cggcaagtcc
      301 tggtcattga gcatttgggc ggacaaccct gaagaagcca gggcgaaatt tcgggctgca
      361 cgagaaaatg cgcactatga cggcgaagtt gtagcaaagg tttatacatt tgtaaatatt
      421 tcgtgggtta agaaattgta caagcggaca aaatatttaa tgggtatcaa agaatgacct
      481 accgtgaatt agttgaacgt cagttggctg tgcgccaagc gggtttgcaa atcgggctgc
      541 aaaaggccaa cgagcaagag ccgttcatcg cggcggtggc cgaaacttta agccgcacga
      601 tgtggggcta tgtgatgcgg atggatgcgc ggtttgaagt aacgtttatc gtagatttgg
      661 gccatgtggc cttcgagcat caattcactg cggtcaaaca ggcgctgaag gaaaaattcg
      721 aggttgaagc cgatggggat gcgctgactg tggaatccga ccgcctgccc tatggtattg
      781 cggcctgccg ggtggtgttc ggagatgtgt tatgagcgga gatacaccga ttaccgtgga
      841 atatgtgttc ggcacgttgg tgtcgtttct gattgcttta ttgtggtatt gggtaaaaag
      901 catttcagac ggcctgaaag aagcccgcaa agatcgtgat gaactgctcg gcaggttgca
      961 cagcgtagaa acatcttatc agacgaaagc ggaagcaaaa gacaacagga acgaaatctt
     1021 aaacttgttg cgtgaaatca aggctgattt gaaagaagtc ggccagaaaa tcgaacggaa
     1081 ggcagacaaa taatgcaaaa ccaagaccct attttaagag cgttggcaga aatcaacggc
     1141 aagcaggaca aattgttaca aaatcaggaa cgcatggatg cggaaatcaa gaaaatccat
     1201 gccgattgcc gccgcacgtc ggcaaccacc ggcgcggccg cgggcgcgat ttcgggcggt
     1261 attgtggcta cgggcatcgc atttgcccgc gccaagttgg ggctgtaacg atggcgcatc
     1321 cgaaggcgac ccgcgacaag ctgcgggcac tctactgcaa cggcgagcag agccttgaga
     1381 cggcggcggc tttatgcggc gtatcgctcg gcaccgcccg ccgctggcgc gatgaggcca
     1441 aggcgcaggg cgacgactgg aacaagctgc gcgcggccca tacgctggcc ggcggcagca
     1501 tagacgaaat cgcgcgggcg atgatgacgt cgtttctggt gcagtatcag gcgacgatga
     1561 cgatgttgca ggatgcggaa gtggaagatt tgccgccgag caaacgggtg cagctgctgg
     1621 cgagtttggc cgatgcgttt accaaaacgg tggcggcgaa caagcgggtg ttgccggaaa
     1681 cctcgcaact ggcgacggct ttggagttgt tgcagttttt gatggtattc gtgcaagaaa
     1741 aacaccccaa acatttggct gcctttgtgg aagtgctgga gccgttcggg gcggaggtgg
     1801 agaggaagtt tggttagttt aatcgtgaag taaacgtgtc atcagaatgt ttaccggcaa
     1861 caccaattgc aaccaataga tctagatgat cggctaattt tttcagtgaa attttaaagc
     1921 cttgctcctc caataaatct tgtatttcag acggcgtgta tttgcccgca tctaacagtt
     1981 tggatatttc taaatcaatc gatttcattt tttgccctta tgaaaaataa agatttcctc
     2041 aaatccctgt ccgccctcgc cgccaatctg cgccaagtca tcgaggccga agtagacggt
     2101 ttcgatgctt cgccgacggc ggtggccgag cggcgggcga aggtatttga cccggtaggc
     2161 ggttacgagt atttcgtcaa cacctacttc ccgcattata tccgttcgcc tgaaaaatcc
     2221 gacctgcacc gcttcttatt caggcgttta ccggaaatcg tcgaatcccc ggaaggcgag
     2281 aacgaagcgg ttggcgcgcc gcgcggcgag ggtaagtcga cgcaggtgac gcagttgttt
     2341 actttgtggt gcattgtaac gggacgcaag cattactgcg tgattgtgat ggacagcata
     2401 gaccaggcat acccgatgct tgaggccatc aaagcggaat tggagtttaa tccccgcctg
     2461 aaaaccgatt ttgcggatgt ctgcggccaa ggccgcgtgt ggcaggcagg taccatcgta
     2521 acggccaacg acatcaaggt gcaggtagcg ggcagcggca aaaagctgcg cggattgcgc
     2581 cacggccctt accgccccga cctgacgatt ttggacgata ttgaaaacga cgaacaagtc
     2641 cgaaatcccg aacagcgcga caagctcaat gcgtggctga cgaaaaccgt gctgccgctc
     2701 ggtggtgtgg ggcaaaaata cgatgtgatt tatatcggca cgattttgca ttacgacagt
     2761 gtgttgaacc gaacgctcaa taatccgttt tggaaaggca tcaagtttaa agccatgttg
     2821 aaatggcccg accgcatgga tttgtgggac agatgggaag agttgtaccg caatgaaggt
     2881 gagttggtgg ccgatgcgtt ctatcaaacg aacaaagcgg aaatggaacg cggggcggaa
     2941 acttcttggg cagcgcgcgg cgtgctggct ttgatgaaaa tccgcgcccg cgacggacac
     3001 gcggctttcg attcggaata tcagaatgat ccggtgagcg gtgaagatgc accgtttgcc
     3061 caagccatgc agttttgggc ggaactgccc gccgatttgg tgtatttcgg cgcgctcgac
     3121 ccttcgctcg gcaaagcggg ggccagccgc gacccgagtg cgattatcgt gggcgggtat
     3181 caaagaagca gcggcaagct gtttatcgtg gaagcgcaaa ttaagaagcg tttgcccgat
     3241 ttgattattg aagacgttat ccgcttgcat gcgcaatata agtgcaagct gtggtttgtg
     3301 gaaacggtgc agtttcagga gttcctgaaa gacgagctgg taaaacgaag tgcggcaaaa
     3361 ggcattcccg tgccggcgcg ggcggtcaaa ccgattgccg acaagctgct gcggattgag
     3421 actttgcagc cgcacatggc caacgggttg attttacttc atgccaatca gcaaacctta
     3481 atccaacagt ttcgccattt tcccaaggca gaccatgacg acggccccga tgcggtgcat
     3541 atgttgtggt cgggggcaac ggccaacagt gcgccggtgg aatatatggc ggtgacgaaa
     3601 catgggacgg acggcggttt cggcagcggc gcatggtgaa actgacaccg ggcagggctt
     3661 acgattaatg caggcagcag agaattgggg caagtttaac ttgccctttt tttatttatg
     3721 aaaaacattt tcagcagtgc attgagcaaa attctgccgg gccgctatac accgaaatct
     3781 accccgcaaa cggcggaaat tacccaaaac acccagactc acgaacaccc gagcaaaggt
     3841 ttgacaccgc agaagctgca cggcattttg gaagctgccg agcgcggcga tatgaaggcg
     3901 cagtcggaac tctttgccga catcgaggag aaagacggcc atatcttttc cgagatgagc
     3961 aagcgcaagc gggcggtcat cgggctggat tggcgcgtga tgccgccgcc gaacagcacc
     4021 gacgccgaac ggcggctggc cgaagaagtc aagggatgga ttgagcgtct gcccgatttc
     4081 gaagacatga tgttcgacct tttagatgcg gtcgggcacg gctttgcctg tgtggaaatc
     4141 gaatggcagc aaataggcgg cctgtggctg ccgaaaaact ttatccaccg tccgcaaggc
     4201 tggtttaagg tggacggtgc cgataatgtg cggttggcca aacaggataa tccggatggg
     4261 gaagagctgt gggcgttcgg ctggctggta cacaaacacc gcagccgttc gggtttgctg
     4321 gtacgcggcg ggctgatgcg cacgctggtt tggccgtatc tgtttaagaa ttattcggtg
     4381 cgcgatttgg ccgagtttct ggaaatctac ggcctgccga cgcgtttggg caaatatgcg
     4441 gtgggggcgg acgaaaccga taaaaccacg ctgctgcagg cggtaaagga aatcggacac
     4501 aacgccgcgg gcattatccc cgaaaccatg aatatcgaat tgcttaatgc cgccaacggc
     4561 agcagcgagc cgtttatggc gatgatcgac tgggcggata aaacatcgtc gaaagcgatt
     4621 ctgggcggta cgcttaccag catggccgac ggtaaaacca gtaccaacgc gctgggtcag
     4681 gtgcataacg aggtgcgcca tgatttgttg gtgtcggatg ccaagcagct tgccggtacg
     4741 ataacgcagc aactgattct gcccctgctg cggctgaata aaggcaacgt ggatgaaacc
     4801 tgcctgccgc gtttccagtt tgatacgcag ctacccgaag atatggcggt ttacgccgaa
     4861 tctttgccta ggctggtaga gatgggcatg aagattccgc tggcgtgggc gcaggaaaaa
     4921 ctggcgattc cgctggcttc ggacgacgag ccggtattgg ctttgcagac ggctgaaagc
     4981 gaaggtgtca aagttgcgcc gttaagttac cgccgcgtgg ccttgagcag gcagggcgaa
     5041 atcttggata tggggcaggc ggccatcgat aacgcaggtt tgggcaaaat cgccctgccc
     5101 gagcatattg agccgttttt gcgcgggttg ggtcaggctt tggccgaggg cgacagctat
     5161 gaagaggtgc aggagcggct gttgcgtgct tatccgcatt tagacagcgc cgaatttcag
     5221 acggccttgg cgcgggtgat tttcatctcc gacctgtggg ggcggctcaa tggctgattt
     5281 gggttttgcc ttcggtttgg agccggaagc ggcggtcaaa tattttgaag gtttgggcta
     5341 ccatatcccg cccgactggg atgtgaaatg gaatgaggcg cagaccaagg cgcggacgat
     5401 tgcaggcata cacaggcagg atattgtcgg cgagtttcac gcggcaatgt atgaggcggc
     5461 gaaatcgggc aaatcgtttg aggcttggcg cgatgaagtg caaggccgtc tgaaagcgca
     5521 tgactggcat ctgctcaaag acggcgatat tgtggacgga gacaccggcg aagtcctcgg
     5581 acgcggtatt accaaacacc gcatggaaac gattttccgc actcagatgc aatcggctta
     5641 catggccggt cattggcagg cgtttgaaga aggtcgggat gatgcgccgt ggctgcaata
     5701 ttcggccatt ttggacagcc gcacccgcca aagccatgcg gcggcgcatg gtgcggtgta
     5761 tcacatcgac gacccgtttt gggattactt ctacccgccc aacggcttca attgccgctg
     5821 taccgtgcgg gcgctttcag acagtgattt gaaacggcgc aatctgctgc cgcaaaaagc
     5881 gcagcttgaa gatacggaag tggtggtcaa ccgcaagggc gatacccgcc ccgccaaagc
     5941 ggtaaagctg ccggacggcc gccgctttta taccgatgcg ggctttcagc acaatgtcgg
     6001 taaaagccat ttggccaact tggggcagtt gcagatgcag cgtgcagtgg aactgccgcc
     6061 gaaactggcg agcgtatcga ttcagacggc cttaaaacag cctgatttga tgcgggcggt
     6121 atcgcaacag gcggcgcaaa tggtacggcg ggtggatgcg gaaaaagtag cgcgcgggaa
     6181 aacgctgtat gtgggcgcat tggccctgcc cgtgttggac gcattggcgg caaggaaaat
     6241 ttacccgcaa tccgccgtga ttgccataag cgacgagcgg gtattgcacg ctttgcgcga
     6301 caagaaggtc aagccgctgc cggtatcgtt ttgggagaag atacccgaac tactgcaaga
     6361 gccggagcag attttattgg gcaaggcggg acgaaatgac gatgccaaac agtttttggc
     6421 atttgtgtat ccgctgtcgg caggcaaggg gaagctggtg gtaacgctgg actatgatgt
     6481 gaaaacccga catccgttga caggtaagaa agaacatctg accttgaata tggtcaacac
     6541 cggcatgatt gcagaaacgg ataaacaggt ggatagctta ctgtacgggt atgaaacaat
     6601 atggaaaaag ccataaaact ctttgcctga ttcgaacagg ataatacgcg acacggtaac
     6661 gtggccgtaa cctttccagt aggaaacccg agttttatgg ctgttggaac caatatacca
     6721 tgattgagat aaaaatcaac acagacaccc tgcaaaacag cttaaacgct gcggcgcggc
     6781 atacctccca caccaagcct ttgatgacgc ggcttgcccg catcatgcgc aacgccgtac
     6841 tggagaattt cgccgccgga ggccgtcccg cttgggcgcc gcgcaaatat ccgactgcgc
     6901 gtgaaggttc ggggctgttg caagccagcg gccgcctgcg caattcgatt acgccgagca
     6961 gtaccaacga tacggcggtg gtcggcacca atgtggaata tgcggctatc cataacttcg
     7021 gtgggaaaac ctcgccacac ttgattagac cgaaaaaagg caaagcgttg aaattcggcg
     7081 gccgctttgc caagcaggtt aaccaccccg gcagcaatat tcccgcacgc ccgtttatga
     7141 ccctgcaacc ggaggacgaa aaggccctat ccgatgcggt ggcggaatat ctggcacagg
     7201 ccattcaggg gcggtaatca tgcctgcctg aaaaatgccg aaaaacagcc cgaaaacgcc
     7261 ctaacccata cttaccccta cccatcgcct gaaaatcaat cctgcgcgcg tttgaacacc
     7321 ttttgaacac tatcccacgc gtatctctgc aagtacattt cccttcccta gaaattcatc
     7381 tgtctgacat aggccaagct ttgacgaggg cgcggctgcc gcacaatgcg ggctatggac
     7441 accaaaaccc ttcttgccgc cttatccgcc gcccatgtcg gcggttcgga cggcttaatc
     7501 aaaatcgtac ccaaaggcca attcgcaccg gttgacggcc gcaccgatac gggtgtgccg
     7561 cactggacga tgtctgccga tttggcacag caaatcattg ccgcctttga cgccgcgcaa
     7621 accgaccttg tggtcgacta cgaacacgcc acgctgaaag ccgccgaaac ggggcagcaa
     7681 aaccctgccg ccggctggat cagcaaatat gtatgggatg acgagcgcgg cctgatgggc
     7741 gaagtgaaat ggacgcaacg cgccaaagac atgatagaca gcggcgaata ccgctatctg
     7801 tcgccggtac tcgaatacga cacgctgggc aatgtacgcg ggctgcacag tgtggcgttg
     7861 accaattcgc ccgcactgga cggcatggct ctggctgcat tgagccgcca aaactctatc
     7921 aaccccaaac aggaaacaag tatgaacaag gaagctttaa tcaagctctt gggcttggcg
     7981 gcggatgccg acgacaaagc catcgaagcg gctttggccg aagcacagga aaagctgggc
     8041 ggtaaaacgc ttgccgaagc actggccgca cccaaagaag aaccgcaagg tagcgaaggc
     8101 gataaaggcg atgccggcaa acccgaagac aagccgcaag gcggcaatgc cgacgacggc
     8161 gaggtagccg aactcaaagc gcaagtggcc gcgttgagca aaaaagtgat tgcaatggaa
     8221 gtgggcggta cttcagacgg cctgatccgt gccgcgcttt cagacggccg cctgttgccg
     8281 catcaagaag catcggcgcg ccaactggcc gccaaagacc ctgaagcgtt taaagccctg
     8341 attgacggca gcttggcact ggccgctttg agcaaaacgc agaccggcgg caaaggcggc
     8401 gcagacggta cgtccgcgct gaccgccgaa gaagcagccg tcgccgcgca attgggcatc
     8461 tctgccgaag attatgcgaa ggccaagtaa tccgataaag gaaaagacaa catgattatc
     8521 acaccagaca ccttaaaagc cctgttcacc ggctttaaaa agaatttcca agacggcctg
     8581 aaaatggcgg aaagccaata caaggaaatc gccaccgtca ttccgtcttc cactgcgtcc
     8641 aatacttacg gctggctcgg ccaatggccc gctttccgcg aatgggtggg cgaccgcgta
     8701 ttccaagata tgaaggcgca cggctatgcc atcaccaaca agcattttga aagctcggtc
     8761 aaggtcaacc gcaacgacat cgaagacgac aacgtcggca tttacgcgcc gatgatgacc
     8821 gagatgggcc gtgcttcggc ggtgcatccc gacgaattgg tgtttgcctt gctgaaaaac
     8881 gcacacgcca cgttgtgtta cgacggtcag aacttctttg ataccgacca tcccgtgtat
     8941 gaaaaagtgg acggcaccgg tcaagccagt accgtatcca atattttcgc cggcagcgaa
     9001 gcggcctggt atctgctgga cactacccgc gccctgaaac cgttgattta ccaagagcgt
     9061 aaagcgaagc agtttaccgc catgaccgcc gataccgacg aaggcgtatt catgcgcaac
     9121 gaataccgct acggcgtgga cggccgttgc aacgtgggct tgggcttctg gcagatggcg
     9181 gcgaaatcgc aagagaaact ggacgctgcc ggtttcgaga aagcctacaa cgcgatggtg
     9241 agcctgaaag gcgacggcgg cagaccgctg gccatccgcc cgaatgtgct gctggtaccg
     9301 cctgctttgg aaaatgcggc caaagagctg gtggaaggcg accgcctggc caacggcgcg
     9361 tacaacccga acaaaggcaa ggcgaaagtc atcgtatcgc cttggttgct gtaacgaaca
     9421 ggcgggcacc gcccgccgga agggatagaa aatggcaaaa gaaaaaaacg gacaggaagt
     9481 tggcgcgacc gtcgatgtcc aaccggaaga ggtaaccgta accgctgcct tacaggccga
     9541 gattgaagcc ttgagagccg aattagacaa agccaatgcc gaaattcagg tagcgcaggc
     9601 agagttggcc gccgcgcaag agcgcaatgc caaattggaa agactgcttg aagccggcct
     9661 tgcacccggc ggcatttcag acggcgaaac tttgacacct gaagcggaag ccgcctttta
     9721 caccggcagc ggagcacccg ccgcagatgc cgaagtggtg gcgattaaga gcaaacacgg
     9781 ccatgcgttt ttccgcgccg gctaccatgt acagccgaac tggacgtttg tgcgccgtgc
     9841 cgacttcgaa cccgccgact ttgagcgcct gattggcgac cgtatggttg aagcgcgcga
     9901 agccctgccg acggacgcat catgagctac gccgccgttg ccgatttggt ggcgcgtttc
     9961 ggcgaagcca ccatcaccgg cctgaccgac ttgtcccgca agggcgcggt ggacgaaacc
    10021 gtcgcacagc aggctttgga tgatgccgcc gccgaaatcg acggctatct gatgaaccgc
    10081 tataccctgc ctctgcccaa gccgctgcgc atcttaaccg tgtattgctg cgatattgcg
    10141 gtgtaccgcc tgtgtaccgg caaacggcag cttaccgaag acattgtgca ccgctatgaa
    10201 gcggcagtga aattcctgca actggtggcg gcggggaaag tcggcttggg cgttaccgaa
    10261 cccgcaggcg aaaagcccgc cgtacaaggc aacggcgtga tgtttaccac acaggaaaag
    10321 gtgttcggtc gtgatagcgt ctattgaaca agccatcagg cagcggcttt cagacggcct
    10381 cggccaaatg gtaacgggtg tctttactta cggcggcgag ttcgacggcg aaggtttggc
    10441 gcaggtggtc aaccagttcc ccgccgtgtg ggtcatgttt gccggcatca aagacaccat
    10501 ccgccacgat acgcgcggca gccgttttaa agcgattggc caattcacgg tattggtggg
    10561 agaccgtgcc agcggcagcg aagcagacag ccgtttcggc ggcctgcacc gccatgatgt
    10621 cggcacctac cgcctgatgc agacctgccg ccacctgctg accaatcaat cgctgggctt
    10681 acagatagac cgtctgcaac cgggggcggc caaaagcctg ttcagccgcc aaatggagca
    10741 agacgccgtt agtgtgtttg cgctggaatt tgaaacgtat tggtttgaag acgcgctgca
    10801 agacggcgat tggccgcggc ctgcggtttc agacggccaa caggcgaaag tatatgccga
    10861 cgtatccgaa taccaaggcc gcaccgaacc cgaacatccc gactttaaag gcgtgaacct
    10921 tgaattgcgt atcccgccga aaacccccga ccaacccgcc gatatggcgg ccaccgttga
    10981 aaccaaggtg aaaccatgaa cgaaaccatt aaagtacgcg ccgccgacgg cctgcaagtg
    11041 ccgctggccg gtaaaccaca tgaatacatt accgaccaag aaaccgttac cgtacccgat
    11101 gccgcctatt accgccgctg tatcgaatac ggcgaccttg tggtcgtaac cgaagacacc
    11161 caacccgcga aaggcagcaa ataatggcat ccgcaaacat caatttcgaa aaaatccccg
    11221 ccagcacccg caagccgggc gtgtacgccg agtggaacac caagctggcc gtgcgcaacc
    11281 tgcccaccaa caaacagcgc gtgttgattg tggcgcagca caacaatccc gctttgggtg
    11341 agcttaccga gctggaaaac gtgttttccg ccgccgatgc ggccgccaaa tacggcgcag
    11401 gcagcatggc gcacctaatg gttaccgccg cgattaaagc ctatgcctat gccgatttaa
    11461 gcctgattac cgtagccgac aacaaggccg gagtggccgc cggcggtaaa atcaccttaa
    11521 gcggtactgc caatacccaa ggcgtgttgc gtgtgagcat tgccaatgct gacactttaa
    11581 ccatcggcat cggcgccgaa gacaccgcag ccaccgttgc cgccgccgtc aaagccgcca
    11641 tcgatgccgt gcccgatttg ccggttaccg ccaccgttgc cgaagcagtg gtgacgctta
    11701 ccgccaaaaa caaaggtacg gccggcaacg ccatccgcat caaaaccagc aataccgccg
    11761 aaggcattac cgccgccgtt accgccatga cgggcggcga tgccaacccc gatattgccg
    11821 ccgcgctcaa tgccgtagtc gccgaaggcc accacatcat cgcctgcggt atcaacgacg
    11881 aaaccaacct gctgaaactg cgtgcccatt tggataccgt agccagcccg atggaaaaac
    11941 gctgggcaat atgcgtatac ggccaaaccg gtacgctggc gcaagccacc accttggcag
    12001 gccgtctgaa ccacggccat atcgtcagcg cgtggtatcg cggcaccccc agcctgcctt
    12061 gcgagctggc cgccgccttt gccgctgtga tggcgagcga agaagacccc gcccgcccgt
    12121 tgaacacgct ggcattaaac agcatcggcg tgtgcgaaag caaagacaaa accatgcgca
    12181 ccgagcagga aaacgccctt tataacggtg ttaccccgat tgaaaccagt ccggccggta
    12241 cgcaggcgca aatcgtgcgc gccatcacca cctacaccaa aaccgccaac ggcacggcag
    12301 acgaaagcct gctggacgta accaccgtgc gcaccctgat ttatgtaagc cgcgcctgcg
    12361 tcgaccgcat cgccctaaga ttcccgcgcg acaaattgag cgaccgcacc ccgccgcgcg
    12421 tgcgcagcga actgattgac gtattgatgt gctgcgaaga gctggaaatt ttggagcgcg
    12481 tggaagaaaa cctgccgaaa ctgattgtgg aacgcgacct gcaaaacacc ggcatgttga
    12541 actgccgcat tcccagcgat gtcgtcaacg gcctgcatgt agtaggtatg gttgtagatt
    12601 tatatttgta agaaaggctg aaacatgagt accgaatacg ttggcagcgt catcctctat
    12661 ctgggcgcga acgaagtcga agtaaccaaa atcgacgtga aagacaacac cggcaaaaag
    12721 cccgtaaaaa ccatgaaccg cacccgccgc gtcaaaggct ttacccgcgg cgtcggccaa
    12781 tacgacatta cctttaccgc cgtggtgccg accgacggca cggcggtgga ctgggcaaag
    12841 atcgacgacg ccaaaatcag cctagttccc gacgtggaag gcgcgcgccc gacatcgtac
    12901 ctcggcttct gcgccaccga agcgggtgac agctataccg tagataatga gctggtggtg
    12961 gatgtgaccg gttttgcgat tcggaaggtg ttggagtaaa gattttcttg ttaacagaag
    13021 ccgctgaagc ggcttctgtt gtttttatta cactgttttt aataaaattc aatttttcaa
    13081 gtaaaatact tgaattttaa aaaagccttt gatattattt agccacgttg aatgtggcag
    13141 taatgctgca aacacatgaa aaggagcttg aacaatggga ctgactaaat tcggcgcagc
    13201 cgttcgtgag gctcgtcgtc aaactaaaca aactctgcaa acgatggctg ctacattagg
    13261 tacttctcct gcttttttga gtgctattga aactggtcgt agtaaagtgc caatggattt
    13321 tgttaaaaag gttgaagatt tttttgaaaa cttaggccaa ccaatagacg gtttaaagca
    13381 aaaagcgatg gtttctaatg agaatgtatc gttaagcggc ttaagcctgc aacaacaaat
    13441 gctggtggct ggttttgcca gttcagactt cagcaaagag caattggaca aatttgctga
    13501 attattaaga accattcatc ataaagattc acagaaagaa ggggatgatg cagcaaacta
    13561 attattcaat gcgtggtgtg cgtgtgggaa tgttaagcga agaaacgatt cagttttttg
    13621 caaacgaagc tgccaaattt ttggtagtag ataagcgtac tcgtaaaaaa atggatgcat
    13681 ttatggaaat gctggaagag tatggcattg taattgatgt tgttgctgat tctgaatggc
    13741 tagtcattac caacgcaatg tgccataacg gaacgattct gatgcctaat agcctctata
    13801 ctcgcatttg caatggcgag gacgaggcta tctttatttt ctttcatgaa ttggggcatt
    13861 tgcttttagg gcataaagcc atgttgcatt atagtgaagt tcttcccacg aaacaggaag
    13921 attcagaatg gcaagctgat gagtttgcaa aatgtatttt aagaaacatg ggaattaaat
    13981 atgtaccaga acagttaagc ttacgatttt aaaaagagaa atgacttgga atgttggcgc
    14041 atcccaagcc attattgtgg tgtactagca tacttccaat atgctgtaca agtgtagttc
    14101 tccatccgct aactgcaaac agatttcaaa gagaaatact tgaaagggac ttgagtatat
    14161 atttttcaga tttgtttgtc cacgaaaagc gaagtgatgg ccaccatttt tacaattgga
    14221 ggtgccatta tggctatgac taaggctccc gttacccccg acttggtgga cgatacaggt
    14281 gcatacattt ttagaaagtc ttttaccacc aaaagtggta agaaaattgt ttctaaaaat
    14341 gggaagccat ttaaaattcc tgtgaaagtt taggaatata taagccccgc taagcggggt
    14401 tttttcatta ctgacgcgtg tttagtcgcc catctcatct ccttaagtga caattaagcc
    14461 ttgatttata caaggtttaa ccaacgttta agggcttttt tatgtctgaa ttttcccccg
    14521 aactcacccg cgctatcgaa gattacgaat tacgcgtttc tcccgacctg aaaaccgttg
    14581 ccggccgtct gaaatacggc atcggtgtga ttgatggcga aatgcaccat gatttttcca
    14641 tgcacctttt aaccgtgcgc gaggacatgg cgattgatcc gcaacttgaa ggccagccgc
    14701 ggctggttgc cgcctatgcc gcgtcactgg ataaattggg tggtttaaca gccgaatctt
    14761 tgacacctga tttgctgctt gatgagatgg ctgctgctga ctttgacgcg ctgtactggg
    14821 cgcaagagct gctgcaaaaa aagcggcttt gcccgcaccc cgcgccgatc gttaccgata
    14881 cgccgtctta aaactcggcc gatacggcat cacgcccgcc caaatcgacg gcatgaccca
    14941 acccgaactg gaaggctggt taaaacaggc cgacttaatc gagcgcggcc gtgccgcgcc
    15001 ggtagtgatg ccgtggttta ttcctgcgaa cgtcaaacca tctgccgcca cgggcggtca
    15061 gacccaaacc tttatcagca agcgtaagaa aaaatgagcc agcatagtgt agaactggtt
    15121 gctaagttta aggacaacgc cacgcccggc ctccgccgtt tggcggcgga agcggaaaaa
    15181 acaggcagcc ggcagttgca gaaagccgca gcagtcaagc tgaaacatca agagatgtac
    15241 agcgctaccg cccgtttggg catccgtact gaacaccaaa tccgccgtga aatccaacaa
    15301 acgcaggcgg cttacaaccg tttggccaaa agcggcatgg cttcgcaacg cgaactggcg
    15361 agggctgcac agataacgcg cagccgtgta cgcgagctga ctgccgaaat caacggcggt
    15421 gcaagccgtt tgcaacgtat cggttcggga gtccggacaa tcgggcgcgg tgtggccggc
    15481 gtggcggcgg gtgcggcggc aggagcttat gtattggcgc agccggtcaa ccggacgatg
    15541 gactttgaca catcattgcg tcatgccgcc aataccatgt atgcaggcaa gagcatggcc
    15601 gagaagcgcg caggtatgga ggaaatcaag aaatcggtca tggatgccgc ctatgtcggc
    15661 ggaacgacgc gtgatgccgc tttggaagcc atgaatacaa tggtggccag cggagcgatg
    15721 agcgacgaag ccgtgaaaaa actgctgccg accgttatga aaaccgccac cgccgccaat
    15781 acggaaggaa acgatattgc caacatcgta accaaggcgt tgcaggcggg gtttaaagag
    15841 gcggatattc cgtcgctgct tgaccgtgcg ttgcaatcgg gcgcggacgg cggttttgaa
    15901 ttgaaagaca tgtcgcgctg gctgccgcag cagttggctg caatgaaaaa cgccggtatg
    15961 ggcgcgacgc tggataattt ttccagcctg ctcaatgcca accaattgtc gtttatgacg
    16021 gcgggcagta ccgacgaagc cggcaacaac ttagtcaacc tgctggcaaa aatcaacagt
    16081 caggatattg tgaccaaagc caaaaaaatt accatcaacg gcaaagaagg tttcgatttc
    16141 accgctagca tgaacaaacg gcaggcagca ggcatgaact cgcttgatgc gctggtggac
    16201 attgtcggag aaatcacggc caaggataag aagtccgccg ctctgatgaa acagattaat
    16261 gccgcgcaag gcgatgaagc caagctggct ttgctggaaa accaaaaggc cttggtagac
    16321 ggcactgcca tcgggaagct ggtttccgac cgtcaggcat tgatggcatt gctggcattg
    16381 gtcaacaaca aacaggaact cacccgcctg caagcaggcc agacaaatgc ggcgggtgcg
    16441 gtggacggtg catacggatt cgtcgccgaa ggttcggggt ttaaaaaatc ccaattctcc
    16501 ctcgccaaat cagaagccga atacggcgca tttgaaaaat tcagcgaccg catcggcggc
    16561 ggccggtaca agctctatgt ttgggggcgg cggcctgctt tccaaactgt tcggcggaag
    16621 cagcagaacc ttacaggcgg gccgtctgat gccctcggcc tcctcgctag gccccgtcgc
    16681 gctcggtgcg acaccgttgg ccgtaatggg cggggcaacc catttggcgg ggcagcgcga
    16741 caaatatgcg gaatggtcaa aaccgtttgc taaattatcg tcttggctgg aaagtatcct
    16801 gcctgatttt tcagcggtgg cgaaagccga atatctgcgc aaacgtgaag aactgggcgg
    16861 caataatgcc ccgctcgata gtcctgtttt aaaagagagt atggcgcagc ttaaccaatc
    16921 ggcacaaacc aaccagcagg caagccagca gtatgtgcag gcggcggcgg agaatcaggc
    16981 ggcaacggcg cagattaccg gcgcggcggc acagatgacg gctgctgccg cacaaatgca
    17041 ggcggcggca ggcaagccga ttccgattac cgttactgtg caaaacggca acattatggc
    17101 ctatgtgaat caggcagtag aacgcaacag taggaaaaat taatggcttg gaaagacact
    17161 ttactggatg cgggcttcaa gggcgttacc tttgacgtca tcgacgacac gctgcgcggc
    17221 acccacgcgc tggcggaaca cgaatacccg ttcgtacagg gcgcggacat cgaagatacg
    17281 ggtgtatctg cgatggatat ggccttgacc gccgtattgt ggggtgaaga ttacgaaggc
    17341 cgcctgcaaa acctgctgaa cgtattgtgc gaaacgggcg cgggcgaatt gatccacccg
    17401 atttacggca gcgtgccaga ttgcgtagtg gcggatttcg aggtcgcgca caacgaagaa
    17461 aaccccgact actgcaccgt gcggatgacc tttaagcaaa gtgtcaaagc cgcgccgttt
    17521 ttcgaccgcg acttgccgat agccttggcc gacgaagtgg atttccttgc cgacttggcc
    17581 gcatggcagg gcttcgaggt gtttcagacg gccttaaaca aaattcagaa agcgcaaagc
    17641 cgctggaatg cttttcatgc cacggtatta atggtggtgg gcgtattgta tgggcaggta
    17701 aacggcatct tcaccggcgg cctgaacctg ctgaacagcc cgcgcgtatt ggtggcggag
    17761 ctgaaatctg tgtttggtgc gctggccaat atgcacaatg tcggtaaaag cgggctggac
    17821 ggctggcgcg acatggtggg cggcgtatct aaagcggccg ctacgccgtg gcaggtcagc
    17881 cgcggaaccg aaggcagtgt ggcggcggta gatttaatcc aacgcgccaa gcccgaagat
    17941 gtggctgcct ttaccgcgct taccgccacc gttggcgcgt gtgcgctggc cgaacaagcc
    18001 gccgatattt tggccgtaca actgaacgag ccgactttga cgcctgtgga aatctcgcgt
    18061 ctgctggccg atacccgtgc cgccttgcag cgtgctttgg ccgcccagcg catcttggcc
    18121 atgatgctgg cggatgaaac caaagccgac aagctggcct attgcctgct gcggctgtat
    18181 caaacaccgg tagacagtgc cgatgatgtg taccaacgca tcgaagtggc cgggctgctg
    18241 ccgcaagccc cttatctgga aactgccgcc gcgcttaccg aaagtctgcg caatacggcg
    18301 cataaactgc aaaaacaggc gtttacagtc atcaatatga agccgccgct ggtacagaaa
    18361 accgtcacct gcgatatcgg cctgcacctg ctggcctttg agtggtacgg cgactacagc
    18421 cgctttggcg aactcttgcg cctgaatccg caaatccgcc acccgaattt tattgagaaa
    18481 ggcacggtac tgaatgccta cgccagataa caccgttacg ctgctgatta acggtaaaac
    18541 acacggccaa tggacgaatt acgacattgt ttccgacctg ctcacccccg ccgatgactt
    18601 ttcagtcatg ctcggccgcc ccgtcgatgc caaacccgat gcggtgcggg cgggcgacaa
    18661 ggtggaagtg cgtgtgggcg gcgatacggt attgagcggc cgcatagacc gcgtgcagac
    18721 cgttaccgaa aaaggcggca aaaccttaac catacaaggc cgcgacgatg cgggcgtgct
    18781 attggactgc tccgccccgc tgtttaacgc gcaagatatg gatttaaacc aaatcatcga
    18841 aaaaatcgtc aagcctttgg gtttggccaa aatccgcatt gatgccgcca aaaccgacaa
    18901 aacccacaaa gtgcagatcg agccgggcag ccgcgcgtgg gacgccctgc tggaatatgc
    18961 cgaggcaaac ggtttgtggc cgtggctgga gccggacggc acgctggtag tcggcggtcc
    19021 cgattacacc gccgcaccgg tggccgaact ggttttacgg accaacggcc aaaacaacaa
    19081 catcaagcgt ctggaagtaa accgcgatat ggcggcacgg tacagcgaag ttaccgtatt
    19141 ggcgcaaagc cacagcggca aaaacaacat caaagccacc gccaaagacg aatccgtcaa
    19201 actgcaccgc cccctaatcg tcaccgagcc ggacatcgac agtcaggctc aagcgcagcg
    19261 caaggcgaaa aagcggctgg ccgacagcag gctggaaggc ttaacaatta ccgccaccgt
    19321 acaaggccac cgcactgacg acggtacctt gtggcagccc ggtcagcgca tcaacgtatt
    19381 gagcgaaccg gacggcatcg acgcggtgta tttcctgatg gcgagaacct tcatcggcgg
    19441 tcgcggccaa cccaccgaaa ccgtgctgac actgaaagag gacggcgcat gggtattgga
    19501 tgccgacccg ccgaagaaat cgggcaaaac caaaaggccg tctgaaagcc gcaaagccaa
    19561 cggccacgca gccgccaaac cgaaaaaacg ccgccaagcc aagcaggccg gacaggaatt
    19621 gcaggtgatt taaatggatg caaaaaccat agacaaccgt atccgacggg catttaacgg
    19681 catccgccaa gccttccgcg gcaaaatcgc ccgcgtcaaa gcagccggcg gcgtgcagca
    19741 aatccaagtg gaaggtttgg aaggcgaaac cgtgcaggac ttggaacacg ccgaaaactt
    19801 cggttttacg tcgaacccgc ccgcaggcag cgattgcgtc gtcgtaccgt tgggcggcaa
    19861 aaccagccac ggcatcatcg tcaccaccac caacggcgca taccgcatta ccggccttgc
    19921 cgaaggcgaa acggcggttt acaacgccga cggtgccaag atggtgttaa agaaaggccg
    19981 gattatcgaa attgactgcg aaacgctgaa tatcaaagca ccgggcggcg tgaatattga
    20041 cgcgcccaat gtaggctgca ccgcgcagat taccgccgag ggtcaaatca acggcaacgg
    20101 cggcatggca gtgaaaggcg gcagcggcac atcatttacc ggcaatgtgc agatggtcgg
    20161 cgatttggac actaccggca aactgaccaa caacggcaaa aatgtcggct ccgaccacaa
    20221 acacaacggc gacagcggcg gcacgacatc cgacccgctg taattcagac ggcctgacat
    20281 ccatcaaacc aaactccaag cgtccttacc tgaaaataaa ggtatggacg ctttacttaa
    20341 tccccaaacg ggcggctatg tggtcaacca atccgcccaa tccatcgaaa acgagttgta
    20401 tatccgcttg gtcacgccct tgggcagtta ctgggcagac cgcacgctcg gcagccgcct
    20461 gcacgaattg cgccgccaaa agcatttaaa gcgcatcgaa gtactggcca aacagtacgc
    20521 cgagcaagcc ttgcagcccg tgattcagtc caaacgcgcc caatccatcc aagtaaccgc
    20581 atccgccccg cagcacggct ggctgaagct gcatattgaa gccgtcgatg ccgcgggcga
    20641 tacagtaacc ctgaaccaca aggtagccgt gatatgacgc aggcactgaa tttagaacaa
    20701 atccgcgcca actatctgcg cgacctgcaa aaccaaaacc ctgccgccca tgtacacgcg
    20761 ggcagcgaca accatgtacg cgccaccgcc attgccgcag tgggcgaagg ccaataccag
    20821 catcaggagt ggattttgcg ccaagccttt gccgataccg cagacagcgc ctacctagaa
    20881 aaacatgccg ctaaatacgg catctaccga aaaaccgcca ccttcgcggg cggcaaggtg
    20941 cgtgttcgcg gcgcggtcgg cgcaacggtg ccggttggcc aacagatcaa tgtgggcgat
    21001 aaggtgtatt taaccgccga atccgccgtt atcagtgcgc ttggaagtgc cgaaatagcc
    21061 gttatcgcca ctgttgcggg tagtgctcaa aatcaaaccg ccgaaaccgc ggctacgctg
    21121 caaagcgtac ctgcggggat tgacagttcc gccgtctaac gatggtcggc ggtacagatg
    21181 ccgaaagcga tgagagcctg ttggcacgat atgaagaacg tctgcgccga cccgctgcgg
    21241 gcggtaatca atacgacttc cgcaattggt gcttggaagt accgggtgtg gttgatgcct
    21301 ttatctaccc tttgcgtcgc ggcaacggct ttgtcgatgc cgtcattttg ggtgaaaacg
    21361 gtatccccag cgcagaaaca ctggccgccg tacaagctca tgtcgatgcg gtgcgacctg
    21421 ttacccgtaa aaacggcttt ctagcccttg cgcccagtat ccagaccgtg aatgtggccg
    21481 tcaccattac cttaagcagc ggcacggata ccgatacggc caccgctgcc atcaaatcag
    21541 ctgtaaacgc ctattttgat gccttaaagc ccggagaccc cctaattaaa agccagttgg
    21601 aaaccttaat cagcgaagtg tacggcgtgc gtgaccgtgt cttaaccacc cctgtgggca
    21661 atatcaagcc tcaggagagt gccgaagaca tttactggct gcgcccgggc agtattaacg
    21721 tggagtacac cacgtgatcc atcaaacctt actcgccgcc atgcgtccgc ccgtcagcta
    21781 cgacaccgta ggagaaacgg cagaaatcaa agccgaagcg ggtgtgtttg atattgtggc
    21841 cgaccatgcg gaaggagtga aaaatgcgcc gttccctgat gcggaaaacg attacctgta
    21901 ccgctgggaa gagctgctcg ccatcacccc gcccgcagga gccaataccc aacaacgtac
    21961 tgatgccgtg ctggccaaac tcaatgcctt gggcggtttg agtattgcct actttaccgc
    22021 catcgccgaa tcggcaggct acaccgtcac catttacgaa gaagaccaat tccgcgcagg
    22081 tgaaagctgt gcgggtgatt gtttgaatac cgaagacgcc atctggcgtt ggtgcgtcga
    22141 catcgccgac ggcaaagcca ccgcctatat tttccgagcc ggtcaaagcc gtgcgggcga
    22201 ccgcatcagt gtgtacaccg acccgattat cgaaacgatg tttgaagaat taaaaccggc
    22261 atggacgtat tgccgctttg aatatgaaga agaggtataa aaatggactt aatccaaacc
    22321 ccgaataagc aatttgtcga cggcgaccgc cgcacgcccg gtactcccgt acccgcatgg
    22381 tggctgaacc agttacaagg cgagttgtac agcattttaa acgcggttgg cattgagcct
    22441 aacaaagccg accatgccca agtcttatcg gccattaaaa cgttggccgc cgatgcttcg
    22501 caggttgcca gtatcgatgc tctgcgtaaa tacagcggca caggctatgt gaacgtcaac
    22561 gcctatcacg ccaatacaac agtgggcggc ggcgtgtttg tggcggataa agccgataaa
    22621 tctaccgctg ataacggctg taccgttatt gtttctaccg acggcacgcg ctggaagcgt
    22681 gtgttttcag ggatgcttaa cctgcatgat tttggatatg tggccagcaa aaacaatgca
    22741 ctatccactt tgaatgccgc tgaatctgcc gcgcttgacg tagttgttga ttgcttgggt
    22801 ttgtcaattg atacgggtaa tacctacccg caaaaaaaca aatacacaaa cggcaagttt
    22861 gtgattaacg gcaaaactgt cgatgttcaa taccagccta tcagaagcgg tatcggtcga
    22921 ttcatctccg gaactggtgc agcagccaac ctcaaatcga atgaatggac tggcgcgggt
    22981 ttaatcgtta ttggcgaagg cgcaatggag cagatggaga aatgtgtttc ctcaatcgct
    23041 attggcgacc gtgcgcaggg cttttctaaa gtaagcaggg acaacatcgc cattggggcc
    23101 gacagcctga ttaatgtgca ggccgctact gaatggtacg accagtcacg catggaaggc
    23161 acgcgcaaca tcggtattgg tggtaatgca ggacgcggca tcaccagcgg ttactctaat
    23221 gtgtcaatcg ggcgcaatgc cggacaggga ttgggtgaag gctcgtcaaa tattgcactt
    23281 ggcgcaggcg cgatggctgg tactgctcca gtcggtttta gtggcgacat tgaagttttt
    23341 tggccgtctt cgacctcaag aacaatcgca atcggcgagg ctgtcttgca aacatatcag
    23401 ggccgcgccg ctcaaaccgc aattggtgcc aatgcggcgc gaaatacaaa aaaggccgaa
    23461 aaagttaccg caatcggttc tgccgcgatg gagaatcttg agcgaaaccg cgccccaaat
    23521 ggcggagatg ttgtctagac gggaacggaa gcaggtacct acgcccaatc tggaaaaaac
    23581 atcacgctta catttcccaa cattcgcggt gcgcaagcga cttattgggt gggcatccgc
    23641 cttacatcag gcacggcgca aaccttacaa aacgacgtcg taccggctca ggtcgtatca
    23701 gtgaatggca atacattaat catccaaagc tcaaaagagc tgaccgccac cggcgcggcc
    23761 gaactgaaat acgtttattc tgtaaattca accgctacta aaaacgaaga gttgaccatc
    23821 atcggcgcga acgccatgaa taaggcattg accgcaggat actcaactat catcggcgca
    23881 gatgccgcgt tgttgggaga caattatcaa aaaacaaccg caatcggcgc atcatcttta
    23941 cgaacaggta gtcatatttc cacaactgct attgggtatt gggtaatccc tttggcaagt
    24001 agtgagaaat gtgttgccat tggagatagt gcgggctatc ggaacgttca aggcgacttt
    24061 ttgactggga aaataacaaa ctccatcgcc atcggatatg gcgcaagaat aaacggcgat
    24121 aacgaaatcc aaatcggtac gacagggcaa actttatatg ctccaaccgc ggtgaacatc
    24181 cgttctgacg gccgcgacaa agcagatgtt aagccgttga cgaacggttt agattttgta
    24241 atgaagctca agccgatgac tggctactac gaccgccggg attcctacgt tgacgaatta
    24301 ttcaaagact tgccggcaga tgaacgagcg gacaaagtcc gcgaatggtg ggcgaatcca
    24361 atcaaggacg gcagtcataa agaagatcgg ttgcggcatt ggtttattgc ccaggacatt
    24421 gctgcgctgg aagatgaata tggtcgattg ccgatggtaa ataaaacaaa cgatacctac
    24481 accgtcgaat acgaaacgtt catccccgtt ttgactaaag ccattcagga aatggccgca
    24541 agaattgaaa cattagaaac cgaaatgaag gaatcgaaaa aatgacaaga tgtgtgattg
    24601 atcaagacgg cttgttcgta gaggaacagt attttgatga cggccgccaa agcatcgaag
    24661 ctgaaatgcc tgatctcgca caatatcagg ccgcccagtg ggatggacaa ggttggaaga
    24721 taattcccga ctatcgcgga tgcgtagttt ttgccggtgg gcaagagcag gtgtgggata
    24781 agttaggtga tttgcccgat ggcgtcagcc tgaccccgcc tgaatcggta aatattgacg
    24841 gcttaaaatc cgtaaaactc gtcgcattaa atgctgccgc tcaggctttt attaacaagc
    24901 acgccggtat cgacagcgta cctgaatttg agtttgcaag ctgggcaatt caagccgctg
    24961 aagcgaaggc ttggcaggaa gataaagccg cgccaacgcc agtgcttgac ggcattgcca
    25021 ccgcccgcgg tatgtcagca gacacgctta aagcagcggc tttgcgtaaa acgctggctt
    25081 acgaacaact cgccgcacat gtggcaggtc aacgacaagc gctgcaaagc aaaatcgaag
    25141 cagcgaaaac gcaggccgcg cttgataaga ttgcagtcgt attcacactg ccggaggccg
    25201 tctgaatggt tcaagtctat ttggcactct ataaaggcaa agccgcaatc aacaccccgc
    25261 gcgatgtggt taaacgcatt gccgacagcg ttgtacgatt ggcaacatgc agcccgtaca
    25321 gccattgtga aatcgctgtt aagcacccac gcgacggcct gtttgattgt tattcgtcta
    25381 gcgcgagaga cggcggggtg cgcattaaaa ccatgccgct gcctgctgat aaatgggact
    25441 taatcccgct gccgcaatct gttgccatat cggccagccg cttgttccac cgtacacacg
    25501 gtgcaggtta cgactggcta ggtgcgattg gcgtggtact caaatcacca cacagcaaaa
    25561 gccgctggtt ttgcagcgaa tggtgcgcat atgtaatagg ctacactaac ccgtgccgat
    25621 acagcccgca aaccctgtat gccgcggtat caactaaaga taggccgtct gagaaaatgg
    25681 aggaaacaaa gtaatttaga aagttttaaa taaagaggag cggcgacgtg tctgtgttgc
    25741 gagcaccggc acgccagcca agcagatgta ccctgcattg acttcaaggc cgctttgcct
    25801 agctaggcgg cggtaattct aacctaaacg gagttaatgc gacatggttt attatcgtga
    25861 attacgttgt gtctactgca aaaaaactat tggccaaagg cagcggtaac gtacaaatca
    25921 agtgtaaccg ttgtaaaacg gttaatactt tcagctagac aaccattact aagaatgccg
    25981 tcgagcatca ttttaaaact gatttcagaa caccagcgag agtgtcggag agtaagtaaa
    26041 aatgatgcaa aaataccact caacggcccc gctgccattc gtcggacaga agcgatattt
    26101 cattaaacac ttcactaaag tattgtcgca aattcccgct gacggcaaac attggacaat
    26161 tgtagacgta ttcggcggca gcggcctgtt ggcgcacgtt gcaaaacgta tcaaaccgca
    26221 ggcgcgggta atttacaacg actatgacaa ctactcagac cgcctgcggc acatcccaga
    26281 ttacaaccgt ttgcgtgaac aaatcgcgca gatagttggc ggcatcccca aaggctcaag
    26341 gctagaccct gaacgtaccc gatcagtgca acaaacaatc actaatttcc aaggccacat
    26401 tgatgtgcgc gtactttcat catggctttt atttagtgcc aaacaggcaa attcgcttga
    26461 acaattgctg gggtttgagt tctacaataa ggtacgccaa tccccatact ccatcgctgc
    26521 cgactattta gacggcctcg aaatcaccca gcaagactat aatcttttga tggctgagca
    26581 tcagcataac cccaatactc tgctggtatt agacccgcct tatgtgtcca ctgctcaggg
    26641 tgcgtatgcc gctgataaat actttaatat ggttagcttc ctgcgtatga ttcagtatat
    26701 gcgcccaccg tttatcctat ttagctccac ccgtagcgag gcgctggact acttccaatt
    26761 tttgcaagag tgcgaaccgg acaaataccg gcgcttcagc ggctacaata tcgtttcact
    26821 agatgccaag atgggcaaag gaatcgagta tcaggacaat atgatttata aaatagatta
    26881 acccgcctaa acggcgggcg gtatcaaagt tccaccttat agcgcggcct aactaggtgt
    26941 caaagatttt ccgaaaaggt gctaaagttc gcgccgcctt ataaactgca cccgttttcg
    27001 ggtgcagttt tttattgcgg attgggacgg gaatggaaaa ggatgtggat tttttatttt
    27061 aatcggctat acaattgctg ctttcatgcc gtctgaagca cgtttcagat ggcatcggtt
    27121 tacatcagat aaggataaaa atgggtattt acgattttca aatgaaagat gcagaaggca
    27181 atgcggttga tttgtcaggc tatcgcggca aggttctgct gattgtcaac acggcaacgc
    27241 gttgcggtct gaccccgcaa tacgaggctt tgcagaagct gtatgcacaa tataccgcag
    27301 aaggcttgga gattttggat tttccgtgca accagttccg cgaacaggct cccgaaagca
    27361 gcggagaaat tgcccaagtg tgtatgatga agtttggcac gaagtttaag attttcgaca
    27421 aaatcgaagt caatggagca aataccgcgc ccttgtatgc ctacctgaaa tccgtcaaac
    27481 cgcaagataa aggcaatcat ctgtttaaag atttcgtgtt gaagctcgca gctttgggag
    27541 agaagcgcga cgaaggcgac atcaaatgga actttaccaa attcctcgtc aaccgcgacg
    27601 gcgaagtggt cgagcgtttt gcgcctagtg ttacgcccga agaaatcgaa gccgatattc
    27661 gggcattgct gtaatctgcc gccgtcttgg gaaatgccgt ctgaaggctt cagacggcat
    27721 ttgtccgttt gcgggtttat tccgaaatat cggcaacacc gctttcgggg aagatgggca
    27781 aatccggcgg tgggagcagg gtatccaaag tcagggcgga cgcatcgagt gtggggtagc
    27841 cttcaaacgc gaccgaataa ccgctgtttc cgttcatacg gattcgggcg tgcgcgccca
    27901 gagggaaagt gattttgtcg gcaatgtgcc cgaagggaaa gcccgtcagc acggggattt
    27961 tcgccgtgcg cgaaacatgg ttggcaacgg cggaaaaatc ataagacgga tcatagacat
    28021 ctcgaatttt ttccatacgg aaattgccga acacgatggc gcgctgtttc ttcaaaatac
    28081 ccgaaagata cagcgtattg agcatacgtt cgatgcggta gggctgttcg ccgacatctt
    28141 cgaggaacaa aatgccgccg tcgatgtcgg gcatataagg cgtgccggcg agcgaggcga
    28201 ggacgcttaa gttgccgccc cacaatatgc cttcggtttc gacatcggcg cgttggatat
    28261 aaggaacatc aacggtcagg cggttttggg ttgcaccctt gataaaggca tccatcgtaa
    28321 acgcaccggg ggcgggtttg ccaaaatcgc tataagccat cgggccggca aaactcatca
    28381 tattgccttt tgccaacaat gccagctgga cggcgcatac gtcgctgaat ccgaaaaaca
    28441 gcgtgccgtg ttcgcgcatc cttgcgccga gcgaagcaaa atcgatatgc ggcagaatgc
    28501 gtgccgcacc ataaccgccg cgcaagccca tcagcacttt aggtgtggca acgcgaccgg
    28561 aagcgacatc ttggaaatcg gcggcacgtt gtgagtccgt gccggcaaac cgttggaaac
    28621 ggcggctgcc cgcctgttgg ttggttacgg taaaaccggc attgtaaagg cgggttaagg
    28681 ctgtgttgac gcggttggtg tcttcggcaa agcctgacga agcgacaacg cgcagaaggt
    28741 tgccgtccga actttgacgg cgtggcgttt ggagaggcac ggttcgggct ttcacaacgg
    28801 aatggaaaga gggaaggggc ggaacggatg tggcggatgt gccgcaagcc tgaagcagtc
    28861 ccgcgccggc ggcggcggtg caggttttca gaaaacgacg gcgggaggtc ggttcggtca
    28921 taatttccct ttcatacggt ttcagacggc atcggcgggc ttaaagcagc tttttaagct
    28981 gttccgtcca atcttgcggc agggtcatac cgtgttttcg gacggggttt gcccaaatcg
    29041 gcgcagggaa ggacgcatcg ttttcaaagc gggcgataat atgccaatgc aggtgcggca
    29101 cgacattgcc caagctggcg aggttaatct tggcaggttg aaacacttgg cgcatagcgg
    29161 cttcgacttt gtacaccatt tccatcaatt cgccgcgttc cgctgccgaa aggtcggtca
    29221 tttcggcaat atgcttacgc caaatgacgc ggcagaatgc aggcgaaccg ctgtcgttat
    29281 ggacggcgat gacgcgcagc ttgggggttt gcagcaaaat atcttcatct tgtgccgtgc
    29341 agatggggca gggttgggcg gtcatttttc cttattcctg aaagtgttta aaaaacggta
    29401 ggataatttc
//
LOCUS       DAWKOD010000008        27770 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000008 DAWKOD010000000
VERSION     DAWKOD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.008"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..393)
                     /locus_tag="WJK73_000253"
     CDS             complement(<1..393)
                     /locus_tag="WJK73_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ7556796.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTAL"
     gene            complement(670..1458)
                     /locus_tag="WJK73_000254"
     CDS             complement(670..1458)
                     /locus_tag="WJK73_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7556797.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(1460..2374)
                     /gene="bioH"
                     /locus_tag="WJK73_000255"
     CDS             complement(1460..2374)
                     /gene="bioH"
                     /locus_tag="WJK73_000255"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ7556798.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            2430..2927
                     /locus_tag="WJK73_000256"
     CDS             2430..2927
                     /locus_tag="WJK73_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7556799.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            2992..3456
                     /gene="trmL"
                     /locus_tag="WJK73_000257"
     CDS             2992..3456
                     /gene="trmL"
                     /locus_tag="WJK73_000257"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ7556800.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            3467..3589
                     /locus_tag="WJK73_000258"
     CDS             3467..3589
                     /locus_tag="WJK73_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ7556801.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3586..4305
                     /locus_tag="WJK73_000259"
     CDS             3586..4305
                     /locus_tag="WJK73_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ7556802.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHTSANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            4359..4685
                     /locus_tag="WJK73_000260"
     CDS             4359..4685
                     /locus_tag="WJK73_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7556803.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4767..5351
                     /gene="ruvA"
                     /locus_tag="WJK73_000261"
     CDS             4767..5351
                     /gene="ruvA"
                     /locus_tag="WJK73_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ7556804.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            5351..5488
                     /locus_tag="WJK73_000262"
     CDS             5351..5488
                     /locus_tag="WJK73_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556805.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            5514..7370
                     /locus_tag="WJK73_000263"
     CDS             5514..7370
                     /locus_tag="WJK73_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7556806.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPTELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            7375..8298
                     /gene="rsgA"
                     /locus_tag="WJK73_000264"
     CDS             7375..8298
                     /gene="rsgA"
                     /locus_tag="WJK73_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ7556807.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            8325..8501
                     /locus_tag="WJK73_000265"
     CDS             8325..8501
                     /locus_tag="WJK73_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ7556808.1"
                     /translation="MPSETGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            8562..8786
                     /locus_tag="WJK73_000266"
     CDS             8562..8786
                     /locus_tag="WJK73_000266"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ7556809.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            8770..9666
                     /locus_tag="WJK73_000267"
     CDS             8770..9666
                     /locus_tag="WJK73_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ7556810.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGVVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9775..10074)
                     /locus_tag="WJK73_000268"
     CDS             complement(9775..10074)
                     /locus_tag="WJK73_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ7556811.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(10186..11631)
                     /gene="nuoN"
                     /locus_tag="WJK73_000269"
     CDS             complement(10186..11631)
                     /gene="nuoN"
                     /locus_tag="WJK73_000269"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ7556812.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11641..13137)
                     /locus_tag="WJK73_000270"
     CDS             complement(11641..13137)
                     /locus_tag="WJK73_000270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ7556813.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNSHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            complement(13226..15250)
                     /gene="nuoL"
                     /locus_tag="WJK73_000271"
     CDS             complement(13226..15250)
                     /gene="nuoL"
                     /locus_tag="WJK73_000271"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ7556814.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(15299..15640)
                     /locus_tag="WJK73_000272"
     CDS             complement(15299..15640)
                     /locus_tag="WJK73_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556815.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15764..16339)
                     /locus_tag="WJK73_000273"
     CDS             complement(15764..16339)
                     /locus_tag="WJK73_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEZ7556816.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(16373..16678)
                     /gene="nuoK"
                     /locus_tag="WJK73_000274"
     CDS             complement(16373..16678)
                     /gene="nuoK"
                     /locus_tag="WJK73_000274"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ7556817.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16675..17346)
                     /locus_tag="WJK73_000275"
     CDS             complement(16675..17346)
                     /locus_tag="WJK73_000275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ7556818.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(17382..18089)
                     /locus_tag="WJK73_000276"
     CDS             complement(17382..18089)
                     /locus_tag="WJK73_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556819.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(18120..18599)
                     /gene="nuoI"
                     /locus_tag="WJK73_000277"
     CDS             complement(18120..18599)
                     /gene="nuoI"
                     /locus_tag="WJK73_000277"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ7556820.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(18679..19755)
                     /gene="nuoH"
                     /locus_tag="WJK73_000278"
     CDS             complement(18679..19755)
                     /gene="nuoH"
                     /locus_tag="WJK73_000278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ7556821.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            complement(19758..22019)
                     /locus_tag="WJK73_000279"
     CDS             complement(19758..22019)
                     /locus_tag="WJK73_000279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ7556822.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWVDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(22309..22746)
                     /locus_tag="WJK73_000280"
     CDS             complement(22309..22746)
                     /locus_tag="WJK73_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556823.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(22746..24047)
                     /gene="nuoF"
                     /locus_tag="WJK73_000281"
     CDS             complement(22746..24047)
                     /gene="nuoF"
                     /locus_tag="WJK73_000281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ7556824.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DLEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(24437..24910)
                     /gene="nuoE"
                     /locus_tag="WJK73_000282"
     CDS             complement(24437..24910)
                     /gene="nuoE"
                     /locus_tag="WJK73_000282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ7556825.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(24910..26166)
                     /gene="nuoD"
                     /locus_tag="WJK73_000283"
     CDS             complement(24910..26166)
                     /gene="nuoD"
                     /locus_tag="WJK73_000283"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ7556826.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDVFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(26156..26749)
                     /locus_tag="WJK73_000284"
     CDS             complement(26156..26749)
                     /locus_tag="WJK73_000284"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ7556827.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(26762..27244)
                     /locus_tag="WJK73_000285"
     CDS             complement(26762..27244)
                     /locus_tag="WJK73_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ7556828.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(27235..27591)
                     /locus_tag="WJK73_000286"
     CDS             complement(27235..27591)
                     /locus_tag="WJK73_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ7556829.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6945 a         7763 c         6957 g         6105 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact atcatataga tttttatgcc atttggtcag
       61 aaacagcgaa gacaggcagg gaaacgcctt cagttccatc gcgtcttcaa aatcatccca
      121 aacatcgctc aaattctgtt tggatatgcc gtattcccgt ccggcaaaca tcacggtctt
      181 tttgcttttg gcggcttttt tgaatgcctt tctctgtttt tcgggtatct gctgccatct
      241 caactgacgg tacacgtcgt agccgtcgcc ccaaaaagag gcataccgtt gcgtgtcttc
      301 gccgaccagc ggcgcggtat tcccaacccg tacatatccc gtcggcacga cggcacttgc
      361 cttatacatg tgcagaccgc gttcccaata cataaagtct ttgtaaagcg gtttcaaatc
      421 cgccagctcc gccgccccaa gcggtttgtt gccctgccag tccaaagcca gcagggttgc
      481 atatgctttt ttcccaccgt gttcttcaac tcgggtaacc tggcaggcag tgtactgctt
      541 cagtctgaag ttatagacgc aatatacgtc tccggcatcg gcatacatcg gcatttcctt
      601 tatttctatc ggttttcaca gacagatgcc gtctgaaact tggaaacagc ttcagacggc
      661 atctgcacat catctcttcg gaaaaaactg caccacgttc tccccttgcg gcagcatcag
      721 ttttttaacg gcgtgtccgt acactgtttc cagcgtaata ctcaaaccgc cttcccgttc
      781 aaatattgtc ccgacacacg aacgcgcggc gttttcatcg ttccacgaca tcgcccgcca
      841 aacgcccagc gtgtccatat ccgcccaaaa cgtttccgcc tttttgtaat ccaacaccag
      901 cttcgccgta tcggtaacgg ggtcgtaaaa cccgttttca gcaagcatat cgcccaagtc
      961 gtgcatatcg gggaaaagcg cgctgcggct ttcaatgcca ttttctttca gacggcattt
     1021 cagttccgcc aaggtatctc gcccgaagca ggtgaaaaac agcagcccgt ccgtctccag
     1081 cgcgcgcgcc cagttgtgca gcacaggaag gatttgttcc gccgccaaca gtccgagatt
     1141 cgaccacaac atatcggcac acgcttcggg cagcggcgcg gtcggggatt ggcagtgttg
     1201 caccacgccc ttacccgtaa acctttgcca aaaaccgccc ttgcgcacgg ctgcggcagc
     1261 cgccaaaaaa tccgcacggg aatcgtattc ttcaaatacc gcctgcggat atcgtttcgc
     1321 cagcaggctg cggctgatgt ccgcatccgc cccggcaagc aggatatgtt tgggcgcgtt
     1381 gcggacgagt gtcagccgtt ggtcggcatg ttcggcaaga tggcggtgaa cctgccaaca
     1441 tttgtcctgc ggattcatgt caaacccctt cgacaaagtc gcggtacagc gcggcaaacg
     1501 cttccgcatg actcaaaaac ggcgcgtgtg ccgccttttc catcacaacc agcctgctgc
     1561 ctttcaaatg ccgatgcaga tactcaccca tacgcggcgg tgtaatcgcg tctttgccgc
     1621 cgaacaccag cagtaccgga acatctatct tgtccaacaa atgccgcgca tccgcccttt
     1681 ccgccgcgtc caaagcttcc tgtaaggcgg agggcgtgcc gcagcgcgcc aaatcgggca
     1741 ggattctgcc tatgattctg tccgcatcat gcgtgtgcaa aagctggagt tgtagaaact
     1801 gtttgatatg tttggcataa tccgttcgga acgcgccgac cattttgccc aatgcaggcg
     1861 cggcaagccc ttcgggataa tcttcggcag ccgtcagccg tgcgaaactc gccgtcaggc
     1921 agagcgaacg gactttgtcg ggatggcgag ccgccagata cagcgcgacc aatccgccga
     1981 gcgaccagcc gagaatgtcg gcgggcgtat cgatttgtgc ggcaacagcg tcggcggctg
     2041 cctcaatatc gaagggttgc gcgaagggcg cgtcgccgtg tccgggcaaa tcgacggcgg
     2101 acaccggcca cgttgcaggc aggcgcggca tcaaatcgtc gaacacgtgg cggttcgccc
     2161 cccaaccgtg tatcaggtaa acttttttga cggcatcagg catggatttt ctctcttgtt
     2221 ggcggcgcat cgcagacgcg cccactatca ggcgttgcgt attatgccac ggttcgtccg
     2281 gcgtttcaga cggcatctgc gccggctgcg cggcggattt ggcggcatac cgcaccgacg
     2341 cggcaaacag ctgccccctg tgtttcagac acatccaagg cggcgcggtg tgcggcggct
     2401 gtcagaaaaa accgcccgca ttcgaccgga tgtgggcttc gctgcattac gaaccgcccg
     2461 tcagcaatat gatacacgcg ctgaagcact tggctgattt gagcatggtg cagccgctgg
     2521 cagacctgat gatgcagaat ccgcccgacc ggcttgcaga cgaatgtttc gatttcgtcc
     2581 tgcccgtacc gctaagcagg gagcggctgc tgcaacgcgg gttcaaccaa agtgaaagca
     2641 tcgtcgggct gctggcacaa cgctacggct ggcagatact gccccgacac accgttttcc
     2701 gacaccaccg cccgccgcaa agcacgctca aaggcggcga acggcggcga aacatcaaaa
     2761 acgcctttga aatccgcaca ccgataccgg aaaactgtaa tattctgtta atcgacgatg
     2821 tctttaccac cggcgcgacg ctggacgaat tggcaaagac gctgaaaaaa ttaggcgcaa
     2881 accgcatctg ctgctggacg ctggcacgca cgccaatgaa aaaataacta aattttttga
     2941 caccccatcc gccttgtggc aaggttacgc gcctataagt gattgatatt tatgtttacc
     3001 atcgttttat accaaccgga aatccccccg aacacgggca acatcatccg cctgtgcgcc
     3061 aataccggcg cggatttgca ccttgtcaaa ccgctcggct tcccattgga ttccgccaaa
     3121 atgaaacgcg ccgggctcga ctaccacgaa ttcgccagcc tgacggtgta cgaaaacttc
     3181 gacgactgcc tcaaggcact cgcaggcagg cgcattttcg ccctgaccac caaaggcacg
     3241 gcgcgccccg atgaaaccgc gtttcaaaaa ggcgacgttt tactgttcgg gccggaaacg
     3301 cgcggactgc ctgccgacat cctcgacagc ctgcccgccg cgcaaaaaat ccgcctgccg
     3361 atgcagcccg gcagccggag tatgaacctt tccaataccg ttgccgtgat tctctttgaa
     3421 gcgtggcggc aacacggtta cgcaggcggc gtttgaacgc aagttcatgc cgtctgaaaa
     3481 cctatccgga cacattccga accgccgtcc gcactgtacg gcggttcgga acgtatccgg
     3541 cgggcatgat gcccattcgt cttcaactca agaacggaac acgttttgac tttaacccga
     3601 aaaacccttt tccttctcac cgccgcattc ggcacacact cccttcagac ggcatccgcc
     3661 gacgcagtgg tcaaggcaga aaaactgcac acctccgcca accgcagcta caaagtcgcc
     3721 ggaaaacgct acacgccgaa aaaccaagtc gccgaattca cgcaaaccgg caacgcctcg
     3781 tggtacggcg gcaggtttca cgggcgcaaa acttccggcg gagaacgata cgatatgaac
     3841 gcctttaccg ccgcccacaa aaccctgccc atccccagct atgtgcgcgt aaccaatacc
     3901 aaaaacggca aaagcgtcat cgtccgcgtc aacgaccgcg gccccttcca cggcaaccgc
     3961 atcatcgacg tatccaaagc cgccgcgcaa aaattgggct ttgtcagcca agggacggcg
     4021 cacgtcaaaa tcgaacaaat cgtcccgggc aaatccgcac cggttgccga aaacaaagac
     4081 atctttatcg acttgaaatc tttcggtacg gaacacgaag cacaagccta tctgaaccaa
     4141 gccgcccaaa acttcgccgc ttcgacatca agcccgaacc tcgccattga aaaacgccgt
     4201 tacgaatacg tcgtcaaaat gggtccgttt gcctcgcagg aacgcgccgc cgaggccgaa
     4261 gctcaggcgc gcggtatgat tcagacggta ttgaccgccg gttgatgtcc cgcctttacg
     4321 tctttcaaac accaccagat acttcgcagg agcaaaacat gaacttcaaa cgccttctct
     4381 tgaccgccgc cgcaaccgca ctgatgggca tttccgcccc cgcactcgcc caccacgacg
     4441 gacacggcga tgacgaccac ggacacgccg cacaccaaca caacaaacaa gacaaaatca
     4501 tcagccgcgc ccaagccgaa aaagcagcgt tggcgcgtgt cggcggcaaa atcaccgaca
     4561 tcgatctcga acacgacaac ggccgtccgc actatgatgt cgaaatcgtc aaaaacggac
     4621 aggaatacaa agtcgttgtc gatgcccgta ccggccgcgt gatttcctcc cgccgcgacg
     4681 actgaatttg atacaatccg tgccgtctga agcccgaacc ggtttcagac ggcattttgc
     4741 acccgacact tcaggattcg gcacacatga tcagcagact gaccggcaaa ctggttgaaa
     4801 aaaaccctcc gcaaatcgtc atcgatgtca acggtgtcgg ctatgaggcc gacgtatcga
     4861 tgcagacctt ctacaacctg ccgcccgtgg gtgaaagcgt acaactgttt acccagctta
     4921 tcattcggga agacgcacat cttttatttg gttttgccac tgcggaagag cgcaaaacat
     4981 tccgccaact gatcaaggtc agcggcatcg gggcaaaaac cgctttgggc attttgtcgg
     5041 cgatgacggc agacgagctg gcgcaggcgg ttgcagaaga agatgtcaaa cgcctgtctt
     5101 ccgcgccggg aatcggcaaa aaaaccgccg aacgtatggt cttggaactg cgcggcaagc
     5161 tggtcgcgca tacggtaacg gacgggctgt ttgccgccgc acccgccgcc gacgaaacgg
     5221 aagacatcgt cagcacgctg cttgcgctgg gttacagcga acgcgaagca aaagcggcgg
     5281 tcaaaggcgt tccggagggg acggacgtgg gcgaaggcgt gcgccttgct ttgaaaaacc
     5341 tgctgaaata atgccgtctg aaggcggcgc ggcgtttgcc ctgacgaaac cctgcgtttc
     5401 cgccccgcgc tgtcttcccg acggcttttc ccgtaaaatg gcgttattgt ccttcctttc
     5461 agacggcatt gtgtttgatg ccgtctgaaa ctgttttacc gaaatcgaaa acaatgttga
     5521 acaaaatatt ttcctggttc gagtcccgaa tcgaccctta tcccgaagcc gccccgaaaa
     5581 cgccagaaaa aggcttgtgg cggtttgtct ggagcagcat gaccggcgtg cggaaatgga
     5641 tagccgccct ggctgcgctg accgccggca tcggcattat ggaagccctg gtttttcaat
     5701 ttatgggcaa aatcgtggag tggctcggca aatacgcgcc caccgaactg tttgccgaaa
     5761 aaggttggga actggcggca atggcggcga tgatggtgtt ttcggttgcg tgggcgtttg
     5821 ccgcgtccaa cgtgcgcctg caaacccttc agggcgtgtt ccctatgcgc ctgcgctgga
     5881 acttccaccg cctgatgctg aaccaaagcc tcggttttta tcaggacgaa tttgccggac
     5941 gcgtgtccgc caaagtcatg cagaccgcgc tggcgttgcg cgacgcggtg atgacggttg
     6001 ccgatatggt cgtttatgtg tcggtgtatt tcattacctc cggcgtgatt ctcgcctcgc
     6061 tcgactcatg gctgctgctg ccctttatcg gctggattgt cggtttcgct tcggtgatgc
     6121 gcctgctgat tcccaaattg gggcaaaccg ccgcatggca ggcggatgcc cgctcgctga
     6181 tgaccggccg cattaccgat gcctattcca atatcgccac cgtcaaactc ttctcccacg
     6241 gcgcgcgtga agccgcctat gccaagcagt cgatggaaga atttatggtt acggtgcgcg
     6301 cccaaatgcg gctggcgacg ctgctgcatt cgtgcagctt catcgtcaac acctccctga
     6361 ccctctccac cgccgcactg ggcatctggc tctggcacaa cgggcaggtc ggcgtgggcg
     6421 cggttgctac agccaccgcc atggcgttgc gcgtcaacgg tttgtcgcaa tacattatgt
     6481 gggaatccgc gcggctgttt gaaaacatcg gcaccgtcgg cgacggcatg gcaaccctgt
     6541 ccaaaccgca caccatcctc gacaagccga acgccctgcc gttgaaagtg ccgcaaggcg
     6601 caatcaaatt tgaacacgtc gatttctcct acgaagcggg caaaccgctg ctcaacggct
     6661 tcaacctcac catccgaccc ggcgaaaagg tcggcttgat cggacgcagc ggcgcgggca
     6721 aatccaccat cgtcaacctg cttttgcgct tctacgaacc gcaaagcggc acggtttcga
     6781 tcgacgggca ggacataagc ggcatcaccc aagaatcttt acgcgcccaa atcggtttgg
     6841 tcacgcaaga tacctcgctg ctgcaccgtt ccgtgcgcga caacattatt tacggccgcc
     6901 ccgacgcgac cgatgccgaa atggtttccg ccgccgaacg cgccgaagcc gccggcttca
     6961 tccccgaact ttccgatgcc aaagggcggc gcggctatga cgcgcacgtc ggcgaacgcg
     7021 gcgtgaaact ctccggcggg caacgccagc gcatcgccat tgcccgcgtg atgctcaaag
     7081 acgcgccgat tcttcttttg gacgaagcca ccagcgcgct cgattccgaa gtcgaagccg
     7141 ccatccagga aagcctcgac aaaatgatgg acggcaaaac cgtcatcgcc atcgcccacc
     7201 gcctctccac catcgccgca atggacaggc tcgtcgtcct cgacaaaggc cgcatcatcg
     7261 aagaaggcac acacgccgaa ctcctcgaaa aacgcgggct ttacgccaaa ctctgggcgc
     7321 accagagcgg cggcttcctc aacgaacacg tcgagtggca gcacgactga accgatgccg
     7381 tccgaacacc cgttttcaga cggcatttcc acacccaacc ccaaagaaac catgaacgac
     7441 accgcccaaa ttaccgccag ctacggccgc cgctacatcg tccgcacgcc cgacggcaca
     7501 acctacgaag ccagcacccg caaaaaacgc gtcgatttcg cctgcggtga ccgcgtccgc
     7561 atcagccccg tcaacgccga acaagttgtg attgaagatt ttttaccgcg ccaaagcctg
     7621 ctctaccgcc aagacgcgtg gaaaaccaaa ctcattgccg ccaacgttac ccaactcctc
     7681 atcgtaaccg ccgccgtccc gagtccgagc gtgcggctgc tacagcgcgc cctgcttgcc
     7741 gccgaagccg ccggcatccg cgccgtcatc gtcctgaaca aagccgacct gcccgaaacc
     7801 gccctttggc gcgaaaaact caaattctac gaaacgctgg gttatcccgt catcgaaacc
     7861 cgcgcactgg aaaacgccgg cagcctgcgc cccgccctgc aagggcacag caacatcctg
     7921 ctcgggcaga gcggtatggg caaatccacc ctgaccaacg cccttttggg cagccaaacc
     7981 gcccgcaccg gcgacatttc cgccgcactc gactcgggaa aacacaccac cacccacgcc
     8041 cggctttatg atttgaacgg cgaaacccaa ctcatcgact ccccgggttt gcaagaattt
     8101 ggtttacacc acctccaagc cgccgatttg ccgcgctatt tccccgattt ccgccacctt
     8161 gtcgggcaat gccgcttcca caactgcacc caccgcgccg aacccggctg cgccttcaaa
     8221 gccgccgccg aaaccagggc ggcaagcccc gaacgcctcg cctttttgca gggcatcacc
     8281 gacgaactgc ccgggtaacg ccttgccgtt tgcaggacaa aacaatgccg tctgaaaccg
     8341 gattcgggtt tcagacggca tccgtttttc aaaaatgcta caatccgctt tttaccggaa
     8401 catccgaaac tatgttccaa cacgcaggac gacacttaaa gcaccgccct atgtgtcgtc
     8461 ctgatttgga agggcttaca cccctcccaa ataaagtctg attctaccgc cccgaaggac
     8521 agatgtccaa gtggcggggt ttcaaccgaa aaggaaacac gatgaagaaa aacgccccga
     8581 aatcgtttga agaagccttg tcgcgccttg aatcgctgac acaagctatg cagggcgaaa
     8641 tgcccttgga agacgcgctt gccgcctatc aggaaggcaa cgagctggtc agatactgcc
     8701 aaaccaagct ggcgcaagtc gaacagaaat tacaggtttt ggatgcggac gggctgaagg
     8761 agttgaacct tgaatccgac gaatgatttg aaagcgtggc aacagagggc gcaggcacag
     8821 acagagctgc tgctggaacg ctttttgccg tctgaaaacg aaatcccgca cacgctgcac
     8881 gaagcgatgc gctatgcggc tttggacggc ggcaagcgtc tgcgtccgat gctggtgctg
     8941 gcggcttcgg aattgggcgg ggtcgtggcg gatgcggtcg gacaggcaat ggcggcaatc
     9001 gaaatgatcc acgtctattc tttggttcac gacgatatgc cggcgatgga caacgacagc
     9061 ctgcggcgcg gcaaaccgac ttgccatgtg caatacgacg aagccaccgc gctactggtc
     9121 ggcgacgctt tgcaaaccca agccttcgac gtgttaagcc gtccgacaga actgcccgca
     9181 gcacgccagt tggcaatgtt gtcggtgttg gcgaaagcgg gcggcagcgc gggcatggcg
     9241 ggcggtcagg caatcgattt ggcgaatgtc ggcaaacaaa tggttcaagc cgatttggaa
     9301 cagatgcaca gcctgaaaac gggtgcgcta atccgtgcgg cggttttatt gggggcgatg
     9361 tcctgcccga atctgtctga agacagtctg gcggtattgg acgattatgc ggcaaaactg
     9421 gggctggcgt tccaagtcat tgacgatgtg ttggattgtg aagcggacac ggcgactttg
     9481 ggcaaaacgg cgggcaaaga cgcagacaac gacaagccga cttatgtgaa actgatgggc
     9541 ttggaagcgg cgcgctcata cgcacacaaa ctggttgccg aagcggtcgc gctgctcgaa
     9601 cccttcggcg acaaagccct gcgcctgcgg cagttggcag aatttgcagt cgcccgcaaa
     9661 tattaaaacc ggcgtatatc gccggctgaa acgtgccggc acagcgatgc cggcctgcac
     9721 ttgaatgaaa caaatgccgt ctgaacaggt ttcagacggc attcctatgc ctgcctattc
     9781 cctgttgcgc gtgtgccaaa aataccgcca cacgaaacag ggaaacgcaa aaatcaggta
     9841 caggcagacg acggtggcgt aaaactccca accctgatcg tgtaccgctc ccgcacggga
     9901 ttcgaggatg taggcaagaa cggcggtcag cgcgaaacct gccgccagct cgatcaggtg
     9961 gtgtccgaaa tgtttgcgct tgagcggtac gatgccgaac agcttggtcg tgaggaaggg
    10021 ggcgttggca aaaatcaagg caagcagcaa aaggatgtac atggatgcgg tcatggcggg
    10081 ctttctgttt gggcattgtc ggcggcgatg ccgtctgaac tgcgttgcgg aacggcatat
    10141 cttaacaaaa acggcagcct ctgacacggc tgccgttgcg gcggcttaca gcgtgttttc
    10201 caacgccttg gcgcaccagt cgataacggt ttgcggcata atgccccaca gcaccaacag
    10261 cagcgcgttg acgctcaaaa ggaattttgc ggcattgttg ccgccgacgg tgggacgggc
    10321 gcggccggat tcgtcaaaat acatgacttt gaccacgcgc aggtagtaga acgcaccaat
    10381 cagcgacatc acgacggcaa atacggacaa ccaaacatgg ccttgtttca agagcgccat
    10441 aatcacgccg aatttggcgt aaaagcccat cagcggcgga atgcccgcca tagagaacat
    10501 aaccagcagc atcaaaaagg caagccatac gcggtgttgg ttcaaccctg ccaaatcgct
    10561 gatgttttcg cactcgttgt ccccgtccga caacaccatc aacacaccga accctgccgc
    10621 cgccatcagc gcgtaggtaa tggcgtaata gagacccgcc gcaaagccga ccgcgcccgc
    10681 cataaacgcc agcaggataa accccatatg cgatacggtg gaataggcga gcatacgttt
    10741 gatattggtc tgcatgatgg cggcaaggtt gccgaccagc agcgaggcgg cggcaagcag
    10801 ggcaaacatc agagaccagt catgatgcac ggttcccaac ccggtaacga ggatgcggaa
    10861 agcgaaaaca acggcggcga ttttcggggc agtgccgacc agggcggtaa cagaagtggg
    10921 cgcgccgtga tacacgtcgg gcacccacat atggaacggc accgcgccga gtttgaacgc
    10981 aacggcgacg acgataaaca ccagacccag tttcaacagc cattcgtttg cttcttcatt
    11041 gaaggaagag gcgagcacgc cggcaaattc cagcgaaccg gttgcgccgt aaaccataga
    11101 aataccgtag agcagcaggc cggatgccag cgcgcccaga acaaaatatt tcaaggcggc
    11161 ttcggcggca aagccggaat cgcggcgcag ggcaatcagg gcgtaaaggg caagcgacaa
    11221 gagttccaaa ccgatatagg cagttaaaaa atgccccgcg ctcaccatca cgcccatacc
    11281 caagagggca aacagcgaca gggtgtaaaa ctcgcctttg aaaataccgc gcacttggtt
    11341 gtagggcctg gcatagacaa acagggcaaa ggtcaaggca tataaaacca tttttgccaa
    11401 acgcgacata ccgtctgcaa tatacatccc gttgaacgaa gacgtgctgc cctgttccca
    11461 caccgccaac tgcaccacag ccgtaaccgc caccgttgcc aacgcgccgt aatgcgtcca
    11521 acggcgtttg tcatcactga cccacaagtc cgccagcaac aataacacca gcagcgcaag
    11581 cagcacgatt tcgggcatgg cgggcattaa attcaaatca gaccagttca tttacacacc
    11641 tcacatcttg ctttgtgcca catgggcaat caaatcgttt gccgcctgat gcaccacttc
    11701 gataaatgcg ttcggataca ggcccatacc caaaacagca accgccaaca ccgccaaaat
    11761 cgcaaattcg cggcaattga tgtctttcat ttcggcaacg tgcgaattgt ggatcgcacc
    11821 aaaaataacg cgtttgtaca tccacagggt ataagacgcg ccgtaaatca gggtcatggc
    11881 cgccaacgcg cccacccaga aattcacttt gaccgcgccc ataatcacca taaactcgcc
    11941 cacgaagccg gaagttgcag gcagaccggc attggccata ccgaacaaca tcataaacgc
    12001 cgcaaactta ggcatcacat tgaccacgcc gccatagtca gcaatattgc gcgtgtgcag
    12061 gcggtcgtac atcacgccga tacacataaa catcgccgcc gacacgaaac cgtgcgaaat
    12121 catctgaatg accgcgcctt tcaacgccca gtcgtccaac tgcccgtcaa caaacaaaaa
    12181 catcccaagc gttacaaaac ccatatggct gatggacgaa tacgccacca gtttcttcat
    12241 atcggtttgc accaaagcca ccataccgat gtaaatcacg gcaatcaggc tcaatacgat
    12301 gatcacgggg gcaaaatagc gtgccgcatc cggcataatc ggcaggataa agcgcaagaa
    12361 accatacgca cccagtttca gcgtaattgc cgccaacacc atcgaaccgc cggtcggcgc
    12421 ttcaacgtgg gcatccggca accaagtgtg cacagggaac atcggcactt ttacggcaaa
    12481 tgacaggaag aatgccacaa acaaaagctg ttgtacgccc aatggaatct gcttgatgtt
    12541 ttggaaatcg acaatagaga agctgcctgt ctgatagtac aggtaaacca tggcaaccag
    12601 catcaaaagc gaacccatca gcgtatagag gaacagcttg accgacgcat aaacgcggcg
    12661 cgggccaccc catacaccga taatcaggta cagcggaatc agcataccct cgaagaacac
    12721 ataaaacaga atcgcatcct gcgcggcaaa cgcgccgtta atcaaacccg acatcatcag
    12781 gaatgccgcc atatactgcg ccggacgttt ctgaatgact tcccaacctg ccaataccac
    12841 caacagcgta ataaacgcat tcaagatgat aaagagcact gaaataccgt ccacgcccaa
    12901 tgcgtagttg attttcagaa gcggaatcca ctcgtggaac tcggtaaatt gatagccgcc
    12961 gctcaaacgg tcgaaaccgg taaacagggg cagtgttacc aagaaaccgg caagcgcacc
    13021 catgaaggcg agcacacgcg ccagcggcgc acggctgtct ttaccggtcg ccaaaaccag
    13081 cacacctgcg gcgatgggta tccatattgc caagctgagt aggtagttgg aaaacatagt
    13141 ggttaacctg tggttaaaat aaaaatatcg ttgtacgggt tggtttgtca gacgacgttt
    13201 taagagaccg tctgaaacct catccttatc tgaacaatcc ccagaaggtc ataccgagca
    13261 agaccaatac gccgaacacc atagcggcgg cgtaggtata gataaagccg gtttgagctt
    13321 tacgcacttg cgcggcaatc gcgccgacca gtttggcaga gccgttgacg atgccgttgt
    13381 cgataatggc ggtatcgccg actttccaga agaaagtacc caatgcgcgt gtgcctttgg
    13441 cgaaaacgtt gaaatacagg gcgtcgaggt agtatttgtt ttcaaacaaa acgtaaatcg
    13501 gacggaacgt ctgtgcaatt ttcgctggca ggtgcggcag tttgacgtac aaaagccatg
    13561 cgctcaacac gcctgcgata gcaaggtaga gtacaggcga atgcaggctg tgcgacacca
    13621 ttgccaatgc gccgtggaac tcttccttca tgatgtgcat ggtcggatgc gcgtcggcgt
    13681 tgacgaaaat cacatctttg aagaaatcgc cgtaaagcat aggctcgatg gcaacgtagc
    13741 cgatgatgac ggacggaatg gcaagcaaaa tcaaaggcag ggtaacaacc aacgggcttt
    13801 cgtgcggatt gtcgtttttg cccaagctgt gatgttcttc gccatgatga tcgtcatgat
    13861 gttcaggcaa gctgcgccat ttctcttcgc cgtggaacac cataaagtat tggcggaacg
    13921 cgtagaacgc ggtaacgaac acgctggcaa ggacggcaaa ataggcaaag ccgctgcccg
    13981 gcagggtgct gtatttcgcc gcttcgataa tcgaatcttt agaatagaag ccggagaaga
    14041 acggcgtacc gatcagcgac aagttaccga tcagcatggt cagccaagta atcggcatgt
    14101 attttttcag gttgcccatg tggcgcatgt cttggtcgtg gtgcataccg ataatcgcgc
    14161 tgcctgccgc caagaacaac aaggctttaa agaaagcgtg ggtcatcaca tggaacatcg
    14221 ccacggaata ggcagacgca cccaaagcca cggtcatata gcccagttgc gacaaggtgg
    14281 aatacgcaac cacacgtttg atgtcgtttt gaatcacgcc caagaaaccc ataaacaggg
    14341 cggtaatcgc accgatcacc ataatgacag acagcgcagt gctgctcatc tcataaatcg
    14401 gcgacatacg cgataccata aacagaccgg cggtaaccat cgtcgcagcg tgaatcaatg
    14461 cagaaatcgg ggtcgggcct tccatcgaat caggcagcca aacgtgcagt gggaattgtg
    14521 ccgatttacc catcgcaccg acaaacagga gcaaacaggt gacagtaatc aacgaccatt
    14581 ccacaccggg gaaaagctgg atggtggcat tttgcacgtt gggcagataa gcgaatacat
    14641 cttgatagcg caggctgccg ccgaaatagg caagcaccaa gccgataccg agcaaaaagc
    14701 cgaagtcgcc gacacggttg atcaaaaagg ctttcaggtt ggcaaatgtt gcgcttggac
    14761 gtttgaaata gaaaccgatc aagagatacg acaccaagcc cacagcctcc cagccgaaga
    14821 agagctggat gaagttgttg ctcataatca gcatcaacat gctgaaagta aacaaagaaa
    14881 tatagctgaa gaagcgttgg tagccgactt tttcatcgtg catatagccg atggtgtaga
    14941 tatgcaccat caacgatacg cccgttacca cgaccatcat catcgccgtc atcgtatcga
    15001 ccaagaagcc gacggagaaa tccaaaccgc ccatcgtcag ccaggtatag acgttctcat
    15061 caaacttggc acggctgcca ttgaggaatc cccacagcac ataagccgac agcacggcag
    15121 acaccgccac accgagtatc gtaacagtat gcgcaccggc acgaccgatt ttgttgccga
    15181 acaaacccgc aatcaacgag cctgccaacg gaacaagggc aattatcaaa tataaagtca
    15241 tgtcgttcat ttgattgaat ccgattgatt taaaaatcta tgtttgtttc gtacaaaatt
    15301 acttcggaaa aacaaatcca acacgctcca atcgtttgcg tgccacagct aattgctctt
    15361 cagtaaataa atcacaccac ggcttttgta acaccagata ttccatactg tatttcaagg
    15421 cgtggactcc gccaccactc agaatcagct ctgtaaaacc ggtctgagtc ttcttttccc
    15481 ccgtacttaa taatttatcc gccgcctctt taccaccaaa ttcatttaca atttgtaaaa
    15541 atcgtgtcgc cttgtaaggt tgcggcaaat tcaaagcctc ctgataaata tttaacatgg
    15601 ctttatgaaa ttcttgttct aactgatttt tatccatcat tcttcttcca atatttcaga
    15661 ccggattatt cttacccaga atttcttttc tcatccgctc ccgtctgatc acctaccgaa
    15721 tcaggtcgtc tgaaacagtc tgaaatcgct tttcagacga ccctcagcct ttttcatacc
    15781 cttcgtaata atacgactgc tcgatacctt taaagatgat ttcacggttg tccacatcgt
    15841 cggtcaggtt gtcctttaac agaaagcgca gttctaaatc gttgacgggg ctgcgttcca
    15901 tcgcctgcaa atacagggtt ttgcttacat tttgccagtt tacgattttt ttcaggtttt
    15961 tcttcagcac caaatccagc cagatgcggg tacttctgcc attaccctcc aaaaacggat
    16021 gggcaatgtt catttcaaca tatttggcga tgatttcttc aaaagtccgc tcgggcatct
    16081 gctcgatttt aaccaaagcc tcttttaaat acatggcgtt ggcaaaacga aaaccgcctt
    16141 tggaaatgtt gtcttccctg atttgacccg caaaatcata taagccgccg aacaggtaac
    16201 ggtgaatctg ttgcaggccc gcggtggtac cgacttcgat acggtcgatg tcgccgcttt
    16261 caaacaggcg gcgggcatta tgcaggcttt gttcgtctat ggatttcatc gtttttccta
    16321 tcgggttttc agacggcatc ggtgtcttgt caggtctcga cccaacctac ctttaccctt
    16381 tcaactcgtc caaatcggca acattaatcg tttgtcggtt gcggtacacc aataccataa
    16441 tcgccaaacc gatggcagat tcggcagcgg caacggtcaa tacgaagaat acgaaaattt
    16501 gtccggcagt atcgcccaaa tgttgcgaga aggcgataaa gttgaagttc accgccaaga
    16561 gcatcagctc gatggacatc agcaatacca gcacgttttt gcggttcata aagataccca
    16621 ttgcgctgat accgaacagg agcgcaccca ataccagata atgcgtcaag gtaatcatgc
    16681 tttgccctcc ccttccggct tgaggccgtc tgaaacttcg ctctcttcgg cagattcgac
    16741 ttgcggtttg accgcttcca ttttcaccag acgcatacgg ccttgatcgg cgcgtacttt
    16801 aacttggtcg gcaggatcca tgcgtttcgg attaaccgtt ttacggtgaa ccagcgcaat
    16861 cgcggccacc atacccaaca gcagcaatac cgccgccaat tcaaacggca gcagataatc
    16921 ggtataaata cggctgccca aatcgcggat attgttgtaa tcggcgggaa tgtctttcat
    16981 cagcccaaat gcggcaaggt cggttttcgg attgaccaga atcagaatca gggcaactgc
    17041 caacaatgtg ccgaccacac cggcaacagg cgcgtgccgc cagaaaccgg cacgcatttc
    17101 ttcaatgtcg atgttcaaca tcatcacgac gaacaggaac aacaccatca cggcgccgac
    17161 gtaaaccacc accagcgtca cgcccaaaaa ctcagcctgc atcagcatcc aaagcatcgc
    17221 gctcacgcag aaggtcaaca ccagatgcaa agcggcgtga acaggatttt tagcggtgac
    17281 ggttttgatc gcgccataaa gaattatcac tgcaaaaata taaaataaaa tcagttggaa
    17341 agtcatagtc tatgctttct ttattaatca aaaatatggt ttcaagcagt ctgaaaatcc
    17401 tggttacgca aacaatcaaa cttaatcagt tgatccatat ccgcacaaat ccacgttgtc
    17461 gcaattttga ataacaacat ttctatgtga tacggttttg cttgtttacc ataaacttgt
    17521 ttcgccaaat cttgtaacaa tccacaatat tgatgaaacg aaacgtctgg tttccgataa
    17581 ttcggtttga tatcagaaaa atgaaacaat acgttcatat ttcggatttc tagttcccta
    17641 tttctacctt gtggctgaaa aaacaaatct gcatttgtaa atttaaacaa caaccaattc
    17701 aacgaaaaaa tagcgcgtga atcataaatg gcgaatcgct cacaatctat aaaagaagcc
    17761 aatttagata atgatgaaat tttcaacaaa ccactactca attttcccga attaagttcc
    17821 gataaaaatt gacgaataag ctggctattt tcagcagatt gtttaaaact tttaatccca
    17881 ccccattcac ggataatcca ataatgcgtc ttcaagctat catcattttg taattttggg
    17941 gataaatttt cttttaaaaa aatattttgt tcaaaattac ttaatccact tggcaactca
    18001 atcggactat ttctagttct aatttcttca aaattatcta ttggctcatg ttcggctaaa
    18061 tatttcaatg cattaatcag tccttccata tttaaaacct tccaagaagc cccaaagcat
    18121 taacgatacg gcgcgtcagc ggctttgcgt ttggcgattt cagcttcgta tttgtcgcca
    18181 atggccaaaa gaatcggctt ggtcatgtgc aagtcgcctt ttttctcgcc gtggtattca
    18241 aaaatatggg tttccacaat cgcatcggtc gggcaggctt cttcgcagaa accgcagaag
    18301 atgcacttgg tcaggtcgat gtcgtaacgc ttggtgcggc gcgtgccgtc ttcgcgttct
    18361 tccgattcga tgttgatcgc cattgcgggg caaacggctt cacacaattt acacgcgatg
    18421 cagcgttcct cgccgtttgg ataacggcgt tgcgcgtgca gaccgcggaa acgcacggat
    18481 tgcggcgttt tctcttcggg aaaataaatc gtgtctttgc gggcaaaaaa gtttttgagc
    18541 gttacgccca taccttttac caattcgcca agcagaaagg tttttactaa gttagccata
    18601 ttatgttccc tcaaaacagg gattctgtta aatattcaaa actgcgtttt cagacgacct
    18661 tgatacgtcg tctgaaactt atttccacaa attcagcggt gaaatcatcc acagacccaa
    18721 aatcacgatg taggcgaagc cgatcggaat cagcactttc cagcccaaac gcatgatttg
    18781 gtcgtaacgg tagcgtggga aagttgcgcg gatccacaga taccagtaca acaccgccgc
    18841 cattttcacg aacatccaga atgcggaagg cgtgccgata aagccccagc tttgtgggaa
    18901 cggagacaac caaccaccga ggaacatcaa cgatgtcagc gcggaaatca gaatcatgaa
    18961 aatgtattcg gcaaggaaga acagcgcgaa tgcgaagccg gagtattcga cgtggtggcc
    19021 ggcaacgatt tcagactcac cctctgccac gtcaaacggc gcgcggttgg tttcggcaac
    19081 ggcggaaatc agatagacga tgaagatggg gaagagcggc agccagttcc acgagaacac
    19141 ggaaccgccc gcaatacctt tggcttgcgc ggcaacgatg tcggagaagt tcatactgcc
    19201 cgataccatc acgacgcaca ccagcgcggc actcatggcg atttcgtaag aaatgctttg
    19261 cgcggaggca cgcattgcgc ccaggaacga atatttggag ttggaagccc agcccgcgat
    19321 gatcacgccg taaaccgaca gcgaggtaat catcaggatg tacaaaaggc cgatattgat
    19381 attggtcagc acccattctt cgttgaacgg aatcactgcc cacgccgcaa aagacggtgc
    19441 aagcgacata atcgggccga tgtagaacag ggctttgttt gacaacttcg gacgggttac
    19501 ttctttaaac aagagtttga acacgtcggc aaacggctga atcagacccc acgggccggt
    19561 tacgttcggg ccgacacgaa gctgcataaa gccgatgact ttacgttcga aatacgtcag
    19621 gtaggcaacg gtcagaatca gcggaatcag gataatcaca attttgacga tgacggatac
    19681 caccaagcct acggtaatac ccaaatcgcc cagaccgagc gttgcggcaa agaggttttg
    19741 gaaccattct tgcatgatca agctcccgcc agttcaatag tgtccatcaa cgcacccagc
    19801 gcggcatttt cggtatgcag cggcagatgc accacgtttt caggcaaacc ggcatcggct
    19861 ttgacggcaa ccgatacgct tgcgccgttt tgtttggcga cagcaatttg tccgtcttgc
    19921 aggcccaagc gtgccaaagt gttcggattt acacgcgcgg caggcacggc ggcatggctg
    19981 gtttcttgca acggtgcgga acggcgcacg atagaatcag tgtgataaat accgacgccg
    20041 ccgacacgga caaggcggtc tgaggccgtc tgaacgccct cccctgtcca tgtgctacgg
    20101 ttgtccagtt tggacggcag gctttctgca tccagcgcgt ctttcagaat cgcagcggta
    20161 tcgtgatatt caaaaccttt caggtcaaac agattgccca atacgcgcaa tactttccac
    20221 atcggacgcg aatcgccgaa gccttgtacc acgccgtgga aagattgcag acggccttcc
    20281 atattgatga agctgcctga ggtttcggta aacggtgcaa tcggcaacaa cacgtcgcac
    20341 acgtccagca gcgtttcgct gacaaacggc gtaaacgcca tcacgctttt tgcctgtttc
    20401 aacgcggcta cggcttttgc accgtcaacc gtatcaattt ccggctcgac gttgagcaac
    20461 aagactgcct gtttcggcgc gtttgccatt tcggcaacgc tctcgccgga gtttacattc
    20521 aagacatccg caccaacact gttggcggct tgcggcaaaa tacccagcac tgcgccggtc
    20581 gcgtccgcca gctcttgcgc ggcggcataa atggcggcgt aatcaggatg gttttgcact
    20641 tccgcgccca aaatcaccgc tgctttttca gcgtttttca ggctggcggt aacggcgtgt
    20701 tccgcattgg cagacaggtt tttcagacgg cctgcccact cgtcgggatg tgcggcttct
    20761 tgagacagaa gcggcataaa caattcttct ttgctgctgg ccaatacgct caaggccata
    20821 cggtctttgg cggcgcggcg caggcgggcg gtcaggagcg gctgttcttt gcgcaagttc
    20881 gcgccgacta ccaatacggc atcgttgtca gccaaagatt caatgctttg tcccaaccat
    20941 tgcgcacctt taatgccgtc tgaaagacgt ttgtcttgtt ggcgcagacg ggtggcaaag
    21001 tttttaacac ccaaaccgtc ggcgagtttt ttggccagat acagctcttc aaccgtattc
    21061 atcgggttcg cccaaacgcc gacttggttt tgattgccgt ctttggcgat acattcaatc
    21121 gcgctgcgga catattccaa cgcggttttc caatccacgt ccacccactc gccgccctgt
    21181 ttgattttcg ggtttttcag acggctttcg tgatacaggc cttcgtaggc aaaacggtcg
    21241 cggtcggaca gccagcattc gttaatcgct tcgttttcca gcggcaacac gcggcggacg
    21301 gtatggtctt tggtttgtac aatcaggttg ctgcccaaag catcgtgggc ggaaacagat
    21361 ttgcggcggt tcaattccca agtacgcgcg ttgaagcgga acggtttgct ggtcaatgcg
    21421 ccgacaggac acaaatcgat gacgttgccc gacagctcgg tttccaccgt tttgccgata
    21481 aagggcatga tttcggagtg ttcgccgcga ttcaccatcg caatttcttg caaaccggcg
    21541 atttcttcag tgaaacgaac gcagcgggtg cagtggatac agcggctcat ttcctcggcg
    21601 gaaaccaaag gccccatgtc tttgccgacg acggaacgtt tttcttcggt gtagcggctg
    21661 gtggtttttc cgtagcccac cgccaaatcc tgcaactggc attcgccgcc ctggtcgcag
    21721 gtcggacaat caagcggatg gttgatgagc aggaactcca tcacgccttc ttgcgcctct
    21781 cgggcttttg ccgaatgcgt acgcacaatc atgccgtccg ttaccggcgt ggcacaggct
    21841 ggcagaggtt tgggggcttt ttccacgtcc accaaacaca tacggcagtt ggcggcgatg
    21901 gaaagttttt tgtggtaaca gaaatgcggg atataagtac cgagcttgtg cgcggcttca
    21961 atcaccgtcg cgccctgctc cacagatact tgtttgccgt cgatttcgat ttgtaacatg
    22021 gttcgttcct agttacgggt atttgataaa taattcttta tggagatcgt ctgaaaaatg
    22081 ggtttcagac ggccttttga gtttcattga caatcaatac tgtttaaaac gtttactgcc
    22141 accttgccgt cattcccgca caggcgggaa tccattcttg ggttttcggt aactgctttt
    22201 caaatatcgg gttctgtaaa ttccactatg gattcccgcc tacgcgggaa tgacggcaaa
    22261 gttaaatttt tagcattttg cttttaacca atataaaacc aactaaaact ataactcatt
    22321 tattatattt ttaaatgact taaataactc tttaacagca cgctcattgt cttcatctgt
    22381 aggctcaaac ttttcacacc aattaattat atttacatcg ccatgcaata actctgcata
    22441 acatcctact gcatcgcaac taccaccccg tgtccttgac gtgccaaaaa taaaatcaca
    22501 attatgcgaa taaagtttgt ataaatttct cctaacctcg tattcattat caccagatga
    22561 ataaaaacca atcctctgac cttcatgatc aaatactgca acaaaatcta agccgtttcc
    22621 atgtctttca aaaaagacta gaaatttaga aaatttccaa caaatctgat taaaaagcgt
    22681 attgagtgtc gtactcttac ctttgttggc tgcaccgtat agaataaaaa ttttagcttc
    22741 caccattacc accctcccca cttatgctct ttcatcggtc cgccgtgttc gatgtaatgc
    22801 acaaactcat cacggaaatg cttggtaaag ctgcgaacag ggaaaacggc ggcatcggca
    22861 agggcgcaga tggtgcggcc tgccatttgg ttgccgacgg aatccagcaa atccaaatct
    22921 tccattttgc ctttgccttc tacgatgcgg tggacgatgc ggtagagcca gcccgtacct
    22981 tctcggcaag gcgtacattg gccgcaagac tcgtcatagt agaagtagct taagcgttcg
    23041 agggctttga ccatgcacac gtcttcatcc atcacgataa tcgcgccgga acccaacatc
    23101 gagcctgctt tagaaatcga gtcatagtcc atattggtct gcatcatgat atcggcaggc
    23161 aacacgggcg cggacgaacc gccgggaata acggccttga gttttttgcc gccgcgcata
    23221 ccgcctgcca ttttcaagac ttcggcaaac ggtgtgccca atggcacttc atagttgccc
    23281 ggacgctcga catggccgga aatacagaac aatttggtac cgcctgcatt cggaatacct
    23341 ttatcggcaa atgcctgtcc gccatcacgg acaatgaatg ggacggagga aaaggtttca
    23401 gtattattaa tggtggtcgg tttgccgtac aggccgaacg aagcagggaa tggcggttta
    23461 aagcgcggct ggcctttttt gccttccagc gattcaagca atgcggtttc ttcgccgcag
    23521 atgtatgcgc cgtagccgtg gtgggcgaag agttcgaatt ccaaatccga acccaaaata
    23581 tttttaccca aaaagcctgc ggcacgcgcc tgctccaaag cggcctcaaa gcgttggtag
    23641 ccttcgaaaa tttcgccgtg aatatagttg taaccggctt tcgcacccat cgcgtaaccg
    23701 gcgataatca tgccttcaat cagagcatgg gggttgaaca tgatgatgtc gcggtctttg
    23761 aacgtacccg gttcgccttc gtcggtattg cagacgacgt atttttcgcc ggggaaggaa
    23821 cggggcataa agctccattt caagccggtc gggaagcccg caccgccgcg cccgcgcaag
    23881 ccggaggttt tgacttcgtc aatcacatcg gtttgcgaga tgttttcgga caggatttta
    23941 cgcagggcgg tatagccgcc gcgtttgacg tattcgtcca atgtccagca atcgggattg
    24001 gcggtatcca cttggtcaaa aatcacgcct gattggtaaa tagccatttt tggtgtgcct
    24061 gtttgttttc gtatcggttg cggccgctgt ttcagacgac cttaagatgt ctttgtgtac
    24121 cggcttgtaa cgtcgtctga aataaaatct agtttatcaa atcgatattt tcagacgacc
    24181 tttaatcgtt tttgttggat ctcgaccact tgcttgtctg tcgtcattcc cgcgcaggcg
    24241 agaatccatc ctcaatggta agcaatgtct tattaaattc agaaaccgaa tcttaccggt
    24301 ggattcccgc ctgcgcggga atgacggcat ttcggtattt cagtagggcg gattcttaaa
    24361 tccgacattt tgccttttta cccactctgt cggatacaag tatccgacct acgtttaaat
    24421 cgtcgtttca gacgacctac tccaactccg ccagtttctt ctcaatcgct tcttcggtca
    24481 taaagctgca catgctgtgg ttgttgacca gcataacggg ggcatcaccg catgcgccca
    24541 tgcattcgcc ttcgacaagg gtaaacttgc cgtcaggggt agtttcgccg tagccgatac
    24601 cgagtttttg tttgaggtat tcgccggtag ccataccgcc gcgcagggcg cagggcaggt
    24661 tggtacaaac ggtcagtttg tatttgccga caggctcaag gtcgtacata ttgtagaaag
    24721 tggcgacttc gtaggcttgt gcaggcgtga tgccgatata gtcggcaaca aaagcgatgg
    24781 tttcgggggc gagccagcct ttttcggttt gggcgatacg caacgcaccc atgatggcgg
    24841 aacggcgttg gtcggcggga tatttcgcca gttctgtgtc gatttgtttt aaggactctg
    24901 cggataacat tatcggtcaa cctccccgaa tacgatgtcc tgcgtaccga tgatggcaac
    24961 gacgtcggcg agcatgtggc cttttgccat ttcgtccatg ccttgcagat gggcgaagcc
    25021 gggtgcgcgg attttcaggc ggtagggttt gtttgcgccg tctgaaatga tataaacgcc
    25081 gaactcgcct ttcgggtgtt cgacagcggt gtaggtctcg ccctcgggaa cgtgcatacc
    25141 ctcggtaaag agtttgaaat ggtgaatcag gtcttccata cctgttttca tttcggtacg
    25201 tttgggcgga gcgaatttgt ggtttgtggt gatgaccgga cccggattga cgcgcagcca
    25261 gtcggcgcat tgtttgatga tgcggacgga ttggcgcatt tcttccatgc ggcagaggta
    25321 gcggtcgtag cagtcgccgt tgacgccaac ggggatgtcg aaatccattt tgtcgtacac
    25381 ttcgtaaggc tgtgtcttac gcacgtccca ttccacgccg gaaccgcgca acatcacgcc
    25441 ggtaaagcct ttttgcatgg cgcgttcggg ggagacgacg ccgatgccga cggtacgctg
    25501 tttccaaata cggttgtcgg tcaggagggt ttcgagtgtg tcgatatttt tcgggaaacg
    25561 ttcgcagaag acatcgataa agtcgagcat cgtgccttcg cgggattcgt tgagctgctt
    25621 caatactttg gcattgcgga atttgctgcc ctcgtattta ggcatgaagt cgggcaggtc
    25681 gcggtaaacg ccgccgggac ggaagtaggc ggcgtgcata cgcgcgccgg aaacggcttc
    25741 gtacaagtcc atcagctctt cacggtcgcg gaaggcgtaa agaatggcgg tcatcgcgcc
    25801 gatgtcgaag gcatgcgaac cgatgcccat caagtgattg aggatgcgcg ttacttcggc
    25861 aaacatcacg cggatgtatt gggcgcggat aggcacatcg ataccggcaa gtttttctac
    25921 cgccaagcaa tacgcctgct cgttgaccat catggaaacg tagtccagcc tgtccatata
    25981 aggcagggct tgcaggtagg ttttggtttc cgccagtttc tctgtgcctc ggtgcaggag
    26041 gccgatatgc gggtcggcac ggacgatttg ttcgccgtcc agctccaaaa tcatacgcaa
    26101 tacgccgtgc gccgcagggt gttgcgggcc gaagttgatg gtgtagtttc ttaatttatt
    26161 ggccaccgta gttctcctca cggacgatac gcggcgtgat ctcgcgcggc tcaatggtaa
    26221 caggttggta aatcacgcgt ttttgctctt cgtcgtaacg catttccaca tagccggaaa
    26281 tcgggaagtc tttgcggaac ggatgtccga caaagccgta atcggtcagg atgcggcgca
    26341 agtccgggtg gttgttgaac atgatgccgt acatatcgaa ggcttcgcgt tcgtaccaat
    26401 ccgcgctgtt gtaaatatcg actacggatt cgactacagg gaagtcgtcg tctgaaaccc
    26461 agacgcgtac gcggatgcgt tgattgtttt taacggaaag caactgactg acgacggcaa
    26521 agcgtttgcc ctgccatact tcgtttttgt aagtgctgta atcgacaccg cacaagtcga
    26581 ccagaagctc gaaatgcaac tcttcatggt cgcgcaatgc ggtcatgact gaaatatagt
    26641 gttcgggcag acactcgacg gtaatctcgc ccaaagcgga aatgactttg cctgcctgat
    26701 tgcccaaaac gcggctgacg gtttcgtata agttttgaat gcttgccata tcgtcctctc
    26761 cttactcgtc acgcgcgatg gtggaagtgc gcttgatttt ttgttggagc tgaatcagac
    26821 cgtaaatcag ggcttccgca gtcggcggac aacccggcac ataaacgtct accggcacga
    26881 cgcggtcggc accgcgcaca acggaataag aatagtgata atagccgccg ccgttggcac
    26941 acgagcccat agacaatacc cagcgcggct cggcgagctg gtcgtatacg cggcgcaggg
    27001 cgggcgccat tttgttggtc agcgtacctg ccacaatcat caggtcggcc tgacgtgggg
    27061 acggacggaa aatgatgccg aagcggtcaa ggtcataacg cgccataccc gcatgcatca
    27121 tttccacggc gcagcaggcc aagccgaaag taaccggcca caatgaaccg gtacgcatat
    27181 agttcaacac cgtatccgcg ctggtggtga tgaaaccttt tttcaaaacg ccttctattc
    27241 ccattccagc gcaccttttt tccattcgta aacaaagcct accgtcagaa cgacgataaa
    27301 caccagcata gaccagaagc cgtacgcacc caaatctttg aacacaactg cccacggcaa
    27361 catgaacgcg acctccaaat caaacaggat aaagaggatg gcgacgaggt aatagcgcac
    27421 gtcgaacttc atcctggcgt tttcaaaggc ctcaaaaccg cattcgtaag gcgcgtcttt
    27481 ttcggcgtag tggcgtttcg ggcccaaaat cgtgccgagc aggataaaca gcacgccggc
    27541 cgcgaggccg acgaggataa agacaaagac gggaaagtaa gcggacaaca tggttacacc
    27601 caaatccgtt aacaaaattt ctacaataat ttcgtatttt agcgaatttc aaaaaccatt
    27661 aaaaggtaaa tatcggcaaa acgcccaaaa aaacccaata aatacaatca tgttatgata
    27721 acgattctta tttgatttta taaggtcata taaattttta caccgtaatt
//
LOCUS       DAWKOD010000009        27416 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000009 DAWKOD010000000
VERSION     DAWKOD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.009"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(124..2025)
                     /gene="thiC"
                     /locus_tag="WJK73_000287"
     CDS             complement(124..2025)
                     /gene="thiC"
                     /locus_tag="WJK73_000287"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ7556830.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(2116..2215)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2365..2441
                     /locus_tag="WJK73_000288"
     tRNA            2365..2441
                     /locus_tag="WJK73_000288"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2399..2401,aa:Met,seq:cat)
     gene            2623..3498
                     /locus_tag="WJK73_000289"
     CDS             2623..3498
                     /locus_tag="WJK73_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7556831.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            3507..4445
                     /locus_tag="WJK73_000290"
     CDS             3507..4445
                     /locus_tag="WJK73_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7556832.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(5165..6940)
                     /gene="ptsP"
                     /locus_tag="WJK73_000291"
     CDS             complement(5165..6940)
                     /gene="ptsP"
                     /locus_tag="WJK73_000291"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ7556833.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(6940..7209)
                     /locus_tag="WJK73_000292"
     CDS             complement(6940..7209)
                     /locus_tag="WJK73_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ7556834.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(7278..7715)
                     /locus_tag="WJK73_000293"
     CDS             complement(7278..7715)
                     /locus_tag="WJK73_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ7556835.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(7796..8359)
                     /locus_tag="WJK73_000294"
     CDS             complement(7796..8359)
                     /locus_tag="WJK73_000294"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ7556836.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(8428..9252)
                     /locus_tag="WJK73_000295"
     CDS             complement(8428..9252)
                     /locus_tag="WJK73_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ7556837.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            9343..9975
                     /locus_tag="WJK73_000296"
     CDS             9343..9975
                     /locus_tag="WJK73_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ7556838.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(10181..11965)
                     /gene="rmuC"
                     /locus_tag="WJK73_000297"
     CDS             complement(10181..11965)
                     /gene="rmuC"
                     /locus_tag="WJK73_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ7556839.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(12304..13092)
                     /locus_tag="WJK73_000298"
     CDS             complement(12304..13092)
                     /locus_tag="WJK73_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ7556840.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(13107..14456)
                     /locus_tag="WJK73_000299"
     CDS             complement(13107..14456)
                     /locus_tag="WJK73_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ7556841.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(14475..15056)
                     /gene="petA"
                     /locus_tag="WJK73_000300"
     CDS             complement(14475..15056)
                     /gene="petA"
                     /locus_tag="WJK73_000300"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ7556842.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(15179..15928)
                     /locus_tag="WJK73_000301"
     CDS             complement(15179..15928)
                     /locus_tag="WJK73_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ7556843.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            16059..16988
                     /locus_tag="WJK73_000302"
     CDS             16059..16988
                     /locus_tag="WJK73_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7556844.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(17243..17635)
                     /gene="rpsI"
                     /locus_tag="WJK73_000303"
     CDS             complement(17243..17635)
                     /gene="rpsI"
                     /locus_tag="WJK73_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ7556845.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(17645..18076)
                     /gene="rplM"
                     /locus_tag="WJK73_000304"
     CDS             complement(17645..18076)
                     /gene="rplM"
                     /locus_tag="WJK73_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ7556846.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(18254..18433)
                     /gene="ssrS"
                     /locus_tag="WJK73_000305"
     ncRNA           complement(18254..18433)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WJK73_000305"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18439..18744)
                     /locus_tag="WJK73_000306"
     CDS             complement(18439..18744)
                     /locus_tag="WJK73_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ7556847.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(18755..19084)
                     /locus_tag="WJK73_000307"
     CDS             complement(18755..19084)
                     /locus_tag="WJK73_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556848.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(19139..20128)
                     /locus_tag="WJK73_000308"
     CDS             complement(19139..20128)
                     /locus_tag="WJK73_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ7556849.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(20251..22953)
                     /gene="ppc"
                     /locus_tag="WJK73_000309"
     CDS             complement(20251..22953)
                     /gene="ppc"
                     /locus_tag="WJK73_000309"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ7556850.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKTDGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHVDVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            22996..23889
                     /locus_tag="WJK73_000310"
     CDS             22996..23889
                     /locus_tag="WJK73_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ7556851.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWRYFDLPRNPECPVCGAAR"
     gene            complement(24436..24660)
                     /locus_tag="WJK73_000311"
     CDS             complement(24436..24660)
                     /locus_tag="WJK73_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ7556852.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(24661..26049)
                     /locus_tag="WJK73_000312"
     CDS             complement(24661..26049)
                     /locus_tag="WJK73_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ7556853.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(26135..26956)
                     /gene="prmC"
                     /locus_tag="WJK73_000313"
     CDS             complement(26135..26956)
                     /gene="prmC"
                     /locus_tag="WJK73_000313"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7556854.1"
                     /translation="MTFDKWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(26963..27406)
                     /locus_tag="WJK73_000314"
                     /pseudo
     CDS             complement(26963..27406)
                     /locus_tag="WJK73_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5990 a         7521 c         7280 g         6625 t
ORIGIN      
        1 tgtactgtct gtggcttcgt cgccttgtcc tgatttttgt taatccacta tattttcaca
       61 agcgaaagaa tgccgtctga agcctttttt ccgggtttca gacggcattt tttgcttgac
      121 gtttcaactg taaatcttcg cgcctttttt gacgaactcg accgcttttt cctccatgcc
      181 ctgccgttgg gctttttgct tgtcggcgta gtcgcgcact tcctgcgtga ttttcatcga
      241 gcagaatttg gggccgcaca ttgagcagaa gtgggcgatt ttcgcgcctt cggcaggcag
      301 ggtttcgtcg tggaagcttt cggcacgttc agggtcgagg cttaagcgaa attggtcgcg
      361 ccaacggaac tcgaaacgcg ccttgctcag ggcgttgtca cgtaattgtg cgcccggcca
      421 gcctttggcg agatcggcgg cgtgggcggc gagtttgtag gtgatgatgc cggtgcgcac
      481 gtcttctttg tcgggcagcc ccaaatgttc tttgggggta acgtaacaaa gcatcgccgt
      541 gccgtaccag ccgatattgg ccgcgcctat gcccgaggtg atgtggtcgt agccgggtgc
      601 gatgtcggta acgagcgggc cgagtgtgta aaaaggtgct tcaaagcagt gttgcagctc
      661 ttcggtcatg ttttctttga cgcgttgcag cggcacatgg ccggggcctt cgatcatgac
      721 ttgtacgtca tgtttccacg ccttatcggt caattcgccc aaggtgtgca gctcggcgaa
      781 ttgggattcg tcgttggcat cggcaatgca gccggggcgc aggccgtcgc cgaggctgaa
      841 cgacacatca tacgctttca tgatttcgca gatttcgtcg aaatgcgtgt agaggaagtt
      901 ttcccgatga tgtgccaaac accatttcgc cataatcgaa ccgccgcgcg agacgatgcc
      961 ggtgaggcgg ttggcggtca tcggtacata acgcagcaac acgcccgcgt gtatggtgaa
     1021 atagtccacg ccttgttccg cctgctcgat taaggtgtcg cggaacaaat cccaagtcaa
     1081 atcttcggca atgccgccgg ttttttccaa agcctgataa ataggcacgg tgccgatggg
     1141 gacgggcgcg ttgcggataa tccattcgcg cgtttcatgg atgtgcgcgc ctgtggataa
     1201 gtccataatt gtgtccgcgc cccaacgcag cgaccacacc attttttcga cttcttcggt
     1261 caggctggaa gtgacggcgg aattgcccaa gttgccgttg attttgacgc ggaagttgcg
     1321 gccgataatc atcggttcga gttcgggatg gttgatgttg gcggggataa tcgcgcgtcc
     1381 ggcggcgatt tcttggcgca cgaattcggg cgtgatttgg tcgggacggg ttgggatgtt
     1441 cgcgccgaaa ctttgccctg cgtgttgttt caagagtttg gcgtattccg atttttggga
     1501 caattcgtct aattttaaac gttcgcgtat ggcgacaaac tccatttcgg gcgtgataat
     1561 gccttggcgc gcgtagtgaa gctgggttac gttgctaccg cttttcgcgc ggcgcgggcg
     1621 ggtgatttgg ttgaagcgca gatgggcggt tttcggatcg tgtgcgcgtt cgatgccgta
     1681 ttcgctggag agcttgggca ggatttcggt atcgccgcgt tcgttcagcc atgcggtgcg
     1741 gatgtgcggc agaccttgtt tcaggtcgat gtgtgccgct ggatcgccgt acacgccgct
     1801 ggtgtcatag acgggaatcg gcgggttggc ttccgcgcct tgcgccgtat aggtgtcgtc
     1861 ctgacggatt tcacgcaaag gcacgcggat gtcgtcgcgg ctgccttgca gatacacgcg
     1921 ttccgagttc ggatatttga agcggatacc gatgtcttcg ctcaagtcgg caagttcgcg
     1981 cgcttcgttg ccggaagttt tggcggtttt ttttggcgta gtcataaaaa atgctcctgt
     2041 tttctcgttt agaataaaga aacaggagcg ttttgcgttt tcagacggca tttgaaaacc
     2101 aatgctgtct gaaagacaga atccgtgaaa actccccacg caggtattat cccgatcggg
     2161 tgtaaagggt atttctcagc cgcctgaaca tcatgcagca cccctgtttc aatgttaacc
     2221 aaaattaaat cacgaacatg aacttttgta aagaaaataa tatttcaaat caggcataaa
     2281 ccgccggacg gcaaaatttt atgatttttc gcggaagtaa tgtttgacaa cataaaaaat
     2341 ctgccgtata gtttcatctt ctgacgcggg atggagcagc atggtagctc gtcgggctca
     2401 taacccgaag gtcgtaggtt cgaatcctgc tcccgcaacc aaatatcaaa cccctcggtt
     2461 caatgccgag gggttttgtt ttgcctgttt cctgccgcct ccgttttttg ccggattttc
     2521 cttcctgccg caatatcgga acggcagccc gccgtctgtt tgcggttgca aattcaggca
     2581 gtttggctac aatcttccgc attgtcttca agaaagccga ctatgccgac cgtccgtttt
     2641 accgaatccg tcagcaaaca cgaccttgat gccctgttcg agtgggcaaa agcaagttac
     2701 ggtgcagaaa gttgctggaa aacgctgtat ctgaaccgtc tgcctttggg caatctgtcg
     2761 ccggaatggg cggagcgcgt caaaaaagac tgggaggcag gctgctcgga gtcttcagac
     2821 ggcattttcc tgaatgcgga cggctggtca gatatgggcg gccgcttgca gcacctcgcc
     2881 cgcacatgga acaaggcggg gctgcttcac ggatggcgca acgagtgttt cgacctgacc
     2941 gacggcggcg gcaacccctt gttcacgctc gaacgcgccg ctttccgtcc gttcggactg
     3001 ctcagccgcg ccgtccatct caacggactg accgaatcgg acggccgatg gcatttctgg
     3061 ataggcaagc gcagtccgca caaagcagtc gatcccgaca aactcgacaa tattgccgcc
     3121 ggcggtgttt ccggcggcga aatgccgtct gaagccgtgt gccgcgaaag cagcgaagaa
     3181 gccggtttgg ataaaacgct gtttccgttt atccgtccgg tatcccggct gcacagcctg
     3241 cgccccgtca gccggggtgt gcacaatgaa atcctgtatg tattcgatgc cgtcctaccc
     3301 gaaaccttcc tgcctgaaaa tcaggacggc gaggttgcgt gttttgaaaa aatggacatc
     3361 ggcagcttgg ttgccaccat gttgtcggga aacatgatgc acgacgcgca actggttacg
     3421 ctggacgcgt tttgccgtta cggtctgatt gatgccgccc atccgctgtc cgagtggctg
     3481 gacggcatac gtttatagga tgcgccatgc ttgaactgaa cggactctgc aaacgcttcg
     3541 gcggcaaaac ggttgccgac aacatctgcc tgactgtcgg gcgcggcaaa atactcgccg
     3601 tactggggcg gtcgggctgc ggaaaatcca ccctgctgaa tatgattgcg ggcatcgtcc
     3661 ggccggacgg cggcgaaatt cagctgaacg gggaaaacat tacccgtatg ccgcccgaaa
     3721 aacgccgtat ttcgctgatg tttcaagatt acgcgctgtt tccccatatg agtgcgctgg
     3781 aaaatgcggc attcggtttg aaaatgcaaa aaatgccgaa agccgaagcc gaacgccttg
     3841 ccatggcggc acttgccgaa gtcggactgg aaaacgaggt gcaccgcaag cctgaaaaac
     3901 tttccggagg cgagaaacaa cggttggcac tggcgcgcgc tttggttgtc cgcccttccc
     3961 tgctgctgtt ggacgaatcg ttttccagtt tggacacgca tttgcgcggc acgctgcgcc
     4021 gcatgactgc cgaacgcatc cgcaagggcg gcatccctgc cgttttggta acgcattcgc
     4081 ccgaagaggc ctgcacggcg gcggacgaaa tcgccgtcat gcacgagggg aaaatccttc
     4141 aatgcggtac gcccgaaacc ttggttcaaa cgcctgccgg cgtgcaggtc gcccgactga
     4201 tgggtttgcc caataccgac gatgaccgcc atattccgca acatgcggtg cgtttcgacc
     4261 aagacggcat ggagtgccgc gtattatccc gtacctgttt gcccgaatcg ttcagcctgt
     4321 ccgtcctcca tccggaacac ggcatcctgt ggctgaacct cgatatgccg cacgccggtg
     4381 aaatatcggg aaacgatacg gtacgcatcc atatcgaaga cagggaaatc gtccgcttcc
     4441 gctgatgctt cttaaaaaca aaatgccgtc tgaaaacctt tcagacggca ttttttacca
     4501 aagcagccat attttttatc agggctgcaa aatttatccg aaacaacaga aaccaccctg
     4561 ccgtcattcc cgcaaaagcg ggaatctaga acccaacacg gcaaaaattt atccgaaaca
     4621 acaaaaatct tttcatcgtc attcccgcaa aagcgggaat ctagaacgta aaatctaaag
     4681 aaaccgtttt tcccgataag tttccgtgcc gacagaccta gattcccgcc tgcgcgggaa
     4741 tgacgggatt ttaggtttct gatttcggtt ttctgtttta agggaatgac gagacttgag
     4801 atggcggcat ttatcgggag caacagaaac caccctgccg tcattcccgc aaaagcggga
     4861 atctagaacc caacacggca aaaatttatc cgaagcgaca acaatctttt catcgtcatt
     4921 cccgcgcagg cgggaatcta gaacgtaaaa tctcaagaaa ccgttttacc cgataagttt
     4981 ccgcaccgac agacctagat tcccgcctgc gcgggaatga cggattttag gtttctgatt
     5041 ttggttttct gtttttgagg gaatggccga ttttgggttt ctgtttcggt tttctatttt
     5101 gcaagaatgg caaaatttca gattgcgggc attgttaagt atttctattt tttacctgcc
     5161 gtatttattt ccgccccttg aagtcggctt cttcctcgac agacacgctg ttcatctgtt
     5221 tgatcagctt ttccgacttc tcttcgtctt cgcagcggat gactttcaca atatcgcttt
     5281 cgagctgtcc gacattgctg tgcagaatga tgtttttgac gggcaggatg ttgttggggt
     5341 tcatggaaaa acggcgcagc cccataccca ataaaacgcg ggtaaacgcg gtatcgcccg
     5401 ccatctcgcc gcatacggat acgtctttgt ccatgcggtt ggcggtacgg atgacgtgtt
     5461 gcagcatttt cagcacggcg ggatggccgg gctggtagag gtggctgacg ctgtcgtcgc
     5521 cgcgatcgac ggacaagatg tattgaatca ggtcgttggt accgacggag atgaaatcga
     5581 ccagtttcaa aatactgccg acggtcagcg cggcagacgg aatttcaatc atacagccga
     5641 tgccgacttt accgaaggca tcgccgcgtt cggcaagctg gcgttgcgcg gtgtcgaggt
     5701 ggatgaggca ctggcgcact tcggatacgg aggtaatcat cggccacatc atccgcacgg
     5761 ggccgtgggc ggcggcgcgg aggatggcgc gcatctgggt gcggaacatg accggttcgg
     5821 caaggcacag gcggatgccg gtcatgccca gcgcggggtt gaggctgccg ttgggcgtgc
     5881 tgtttttccc gaaccagcgc gggtttttgt ccacacccaa atcgactgtc cgtatcgtta
     5941 cgcttttgcc tttcattttt ttgacaatcg cgctgtacac ttcgtactgc tcgtcttcag
     6001 acggcatcgt atcgcggttc aggtaaagaa actcgctgcg gaacagcccg atgccgtctg
     6061 cgccgaggtt gtgcagcggt ttcacgtctt cggcggattc tatattgccc acaagctcga
     6121 tgcagacccc gtcagcggtg gcggcggcgg tttttttgag cttgttcaaa tcgcgtttgt
     6181 ggctgcggta ttcgcgggca cggcggcggt attcgttcaa caccgactca tccggcgcga
     6241 taatcaacac gccgttgata ccgtccacaa tgaccgtttc gccctcggta atcagtttgc
     6301 gcgcgttgtg cagcccgacg acggacggga tgtccaagct cctgcccaaa atcgccgtat
     6361 gcccggtggg gccgccggca tcggtaacga aggcggcaat gcgctgctct ttaaacaaaa
     6421 ccgtgtcggc gggcgaaagg tcgtttgcaa tcagaacggt ttcgtcaaac aggttgtcgg
     6481 caacttccaa ctcgttgccc tgcccgatca ggttgttgtg gatgcggcgg acgacttgca
     6541 gcatatcctg cttgcgttcg cgcaaatagg catcgtccat attgtcgaat tgggcggcga
     6601 gtttgtcgct ctgctgcttc aatgcccact cggcgttgat tttttgttcc cttaaaatat
     6661 cgacgggttc gcgcgacaag gtaacatcgg tcaagagcat caggtgcagc gagatgaacg
     6721 cgcccaactc ggtcggggcg ttttcgggaa tcgcgctgcg gagctgttcc aactctttgc
     6781 gcgtggcttt gacggcggca tcgaaacgtt cggcttcggc atcggtgtcc gcctccgcaa
     6841 catcatactg cggcacttcc tccgtaccgc gcgcaatcag gtgggcgcaa ccgacggcaa
     6901 tgcctttgcc cgccgccacg ccgtgcagca cgatactcat tattcgccct cgccgaagta
     6961 gccgttgatt aagtcggtca gggcgcgcat cgcttccgcc tcgtccgcgc cgtccgtctc
     7021 cagttcgatg accgtaccct tggcggcggc gagcatcatc agccccataa tgcttttgcc
     7081 gttgacgcgg ctgtcgtttt tcgtaaccca gacttcgctt ttgaattggg acgcggtttg
     7141 ggtgaacttg ttggacgcgc gggcgtggag tccgagtttg ttgatgattt cgatggattg
     7201 tttgagcatt tcgattcccg tgttatgtat atcggcagca gacgccgttt aaaatgtttt
     7261 cctgccctgc cgcttcttca gacggcatcg ccgctgcgcc ggcacaccaa atcttcgggc
     7321 gcggacgtga tggcgaaaat gccttttacc gccgcctccc tgacgcattc ggtaaaggcg
     7381 gcaaggtctt ccgccgccgg cgaatattgg acggccttaa ccatcatcgg cgcgttcagc
     7441 ccggtcaaaa tcgccgattt gttttcgcgc acgaggcggc gggctgcatt gcagggggtc
     7501 gcgccgaaaa tatcggtcat aattagcacg ccgtcgttgt cgggaaactc ctgaagcgcg
     7561 gcaatggcgt tgttgatgat gtcgtcttgg tcttccgtcg gttgcacgcc gagtatgcgg
     7621 acgttttcag gcagtccgcc cggaaaaaaa tgatgtgcca gcttgcggta ggcttcgcct
     7681 atggtttcgt gtgtgatgat taaaagccct atcatattat gcgtcctgtt cctcgttgtc
     7741 ctaccggcgt atgggcgcga tgccgtctga aaggccttca gacggcatcg cgtccttatt
     7801 ttccgtccaa tgcgtaaatc tcgcccagat tgcgccagca gcccgccgca tccatgccgt
     7861 aaccgaaaac ataacggttc ggcacatcca gtccgacata atcggctcgg ataggcttgg
     7921 gtttgtcaat cagcttgttg gcgaacaccg ccgcacggca gcttgccgca cccatttcca
     7981 aaagtttggc ttgaatggcg gacatcgtat gcccttcgtc caaaatatcg tccagcacga
     8041 cgacgtgcct gccccggatt tgttccgcat cgggcatacg cttccagttg aacgcgccgc
     8101 cctccagctt gtcgccgtaa cgggaaacgt gaacataatc aaaatctaag ggaaaacgca
     8161 acagcggcag caactgcccc gtaaacacca ccgcgccgcc catcacgggc agcagcagcg
     8221 gatatttgcc gcccaaatcg cgcgtaatct cgtccgccac tttttgcagt gcggcacggc
     8281 attggccttg gtcgaacaaa agatcagcgt tttcaagcat cgcctgtgtt tcaaggcgtt
     8341 tggtttctaa atcggtcata tgtcggaatc ggtcggtaaa aggaaaatta taaaccaaag
     8401 tatcggatgc cgtctgaact gtcctgctca gcggtcggta cgcacgcgca caaatgtggc
     8461 aaatttcggc gtgcctttcc gcgtaaagcc acggtaacgg taggtaatca gcgtgccgat
     8521 tttgggcggg ttgtcgcggt ctttgtcctt gaaaccgctg ccgatgcgga attcgccgtg
     8581 tcggtttttg cagccgaccg cgcccagccg tccggcgttt cgccctttgc cctcatagtg
     8641 ccgcgttacc gtgcattcgt cgtcgtattg gcttttcagc ttcaataatt ggctgctcct
     8701 gccgccgcta taacgggatt cgggctggcg cagcatcacg ccttcgccgc cctgcgcttc
     8761 gatttgtttt aaaaagtcca tcgcgtgctg ccggtcgcgc actttgattt gcgggatgat
     8821 ggtaatcggc gcgttcggat gcgttttcag ccactgcgtt gcgactgcca aacgttggta
     8881 gagattgccc tgcgccttgg gtacgtcgaa aacgtgcaga cggatgccgc gccagtctga
     8941 agaaacagaa cgcacggtag cggaaatctg ctcgaactga ccgcgtccgc tatacaattc
     9001 gccgtccaaa ggataaggcg gaaactgcgc ggtaaagcct ttggggggcg taaacgcgta
     9061 gccctgacgg cttatcaggt gctttccgtc ccaataggcg cgcacgccgt cgagtttctc
     9121 gctcattgcc cagccggcaa tatcctgccc tttgtattcc tgcgccagca tcaaatccgc
     9181 cgcgcctgcc gatgcaggga tgaaaaccgc cgtaaaaatc ggtatgatgc cgccgattgt
     9241 cttcttaatc atctgattcc ccaaatatca aaacaggcgg caaaccgcca taaaacaaac
     9301 ggcaaacccg atgccgtctg aaaaaccgtt taggaacacg ccatgaccct acgttacgaa
     9361 atcctctccg ttaccccctt ccgccaaaac tgcaccctga tttgggacga cgaaagcggc
     9421 gaagccgtcc tgaccgatgt cggcggcgac gtgccgttcc tgctgcaagc gttggcaaac
     9481 cgcaaactta cgctcacggc aatctggctg acgcacggcc atctcgatca cgcgggcggc
     9541 gtggtcgaaa tgttgaaaac gcataaagtc cctgtcctcg ggccgcatcc ggacgatgaa
     9601 ttcctgctcc aatcgctgcc gcaaaccacc gcgcaatacg gatttcctgt ctcgcccgcc
     9661 tttgcgccga accgttggct cgaagaaggc gaaacgctca cggtcggacg ctatgccttt
     9721 caagtgctgc atattccggg ccatacgccg ggacatatcg tcttttattg tgccgaggcg
     9781 gaattgctga ttgcgggcga cgtgctgttt tacgaaacca taggcagaac cgattttccg
     9841 cgcggcaacc acgccgactt aatcaataat atccgcaaca aattattcgc cctccccgaa
     9901 accgtgcaag ttgtcgccgg acacgggcgt atgacttcca tcggacacga aaaacggcac
     9961 aatccgtttt tctaaccgac ttcactacgg tcttcagacg gcgtcatctg cactgatgcc
    10021 gtctgaaacg caaaaggctc agacaaccgc cgccttgccg gtgtacctcg ccggacaagg
    10081 ctttggtaag tacttcaaac aaaccccaaa tcagaaccgg tacagttacg ccgcgctttc
    10141 ggcattcccg cccctgctga aacaagcttt tccgcacaag tcagactgct tcatcttctg
    10201 ccacatattc caaagattcc gacaacgccg ttgtttcatt ggcacgttcg gtcaaatcgc
    10261 gtgcaagctg cttgtttgcc ttcacgccca acagacgcag tttctcggcg cgtccgacca
    10321 gattcccgcg cccctcggca agttgcttaa atgccgtctg aaagctgttt tgcgcctgat
    10381 cgatgccttt gccgacgctt tcgagcgtct gtacaaagcc gacaaatttg tcgtacagct
    10441 tgccgccttc gtccgcaatc gccagcgcgt tttggttttg ctgctcgttg cgccaaatat
    10501 tcgccaccgt ccgcaaagtc gccagcagcg tactggggcc gaccagcata atccgtttgt
    10561 cgaaacactc ttggaacaag ccagcgtcat tctgcaacgc caacaggtag gccggttcga
    10621 cagggataaa cataaagacg aaatccaatg tgttcacacc ttccaaatcg gtgtaatcct
    10681 tcagcgacaa gcctttcata tgcgcgcgta tgctggcgac atgcgccgcc aactcgcgtg
    10741 ccgccgtatc cgcatccgcc gcctgcgtgt agcgcacata agctgtcagc gagaccttgg
    10801 agtcaatcac aatctgcttg ttgtcgggca ggttgaccaa aacgtcgggc tggaggcggc
    10861 gcgtgccgcc gtcttcctct tttcggacgg atgctgcctg aaccacatat tcccgccctt
    10921 tctgaaggcc ggaattttcc aaaaccgttt ccagaatcat ctcgccccaa ttgccctgaa
    10981 ccttattctg cgtaccggtc agcgcgttgg tcagggcctt tgcctcgctg tgcagctgcg
    11041 cattcaatcc ttgaagccgt ttcaattcgt tttccaacgt cagccgctcg cgcgattctt
    11101 tatcataggt ttgcttgacc aactcgccga aaccttggat gcgttcgttt agcgggttca
    11161 aaacctgatg gagctgctcc cggttctgct cggtaaaacg gcggcttttt tcttccaaaa
    11221 tcgtgttggc aagattttga aactgatcgc tcaaactttt gcgcgcctcg cccagcaagg
    11281 acagcttctc ttcagaagca aggcgttcct gttcgatttg cgttgccaaa cgttcgtttt
    11341 caaccgccaa accctgtgcc ttttcctgca actcggtatg cgactgcctc aaccgctccg
    11401 cttccgcctc tttttcctgc aaatgggcaa tctgtttttc ggctgcggca aaacggttgc
    11461 cgacatcggc aaggtcgttt tgcacgtcgc ggacagtttg gcggctttct tccaaatcgg
    11521 tttcgattct ttggcggatt tggcgttcca aggcatattg gtcggcaaac gccttccgtt
    11581 cctgtccgag ctgcgttgcc aaacgttcgt tttcaaccgc caaaccctgt gccttttcct
    11641 gcaactcgat atacgactgc ttcagccgaa ccgattccgc ctctttttcc tgcaaatggg
    11701 caatctgctt ttcggctgcg gaaaaacggt tgcccaaagc ataattttcg tcctgcaaat
    11761 gtcggtattt cccgtccaaa acggccaatt ccgatgcaat ttctgcgtgt gcctgttcga
    11821 caaaatcaca tcttgccgcc ttttccgcca gttgcgcgtt caaatcggca aactcgccct
    11881 gaaacctgcc cttcatcagc aaccatgtaa acaacacgcc cgacaccaac gccgccaaag
    11941 gcagcaaaac agtcatcagt tccatcaatc atcctaatat tcaaacattt tcacaccgga
    12001 caaaaccgcc gcttattccg attctacctg tttgttcgac atagctccaa aaaatatagc
    12061 ggattggctt taaacctgtt cgacatcgcc ttaccatgct gcttgcggtt tcagaccttt
    12121 tcctaattca atatcaatct gccacaaacc ctgattaagt tcccgatgtc tgacattttt
    12181 agaatgatgc cgtctgaaat gttgcagcta tgttcagacg gcatacggat tcaggctttt
    12241 caaacggcag gcaaaatgaa aaaagggcaa accctaaagg atttgccctt ttgttccaaa
    12301 cgcttagtgt acgtctttcc aatattcttt tttcaggaaa tacgccaaag gcagcataac
    12361 cgcaaatagg aaaatcatca cgacatagcc tatacgtttg cgttgcagtt gtgcaggttc
    12421 gcccatgtac acaaggtaat tgaccaaatc gcgtacatat gcgtcgtact ctttttggat
    12481 cactttgccg ttaggcagac ggcggctgtg caaaccggta gattcccaat acagcttagg
    12541 cttcatctcg ccgtgttcgt cttttaccat aaccggctga cctttggcat ccaactcaac
    12601 ggcttgaacg ccttgctgtt cccacaacgg gtggggcata ccgactttat cgaatacagt
    12661 attgttccag ccgctcggac gggtcggatc tttatagaag ccgcgcatat aagcgtaaag
    12721 gtagtctgca cctttggaac gcgcaatcaa tgtcaaatcg ggcggagcag caccaaacca
    12781 ttttgccgca tctttcgggt tcatcgccga atgcatgaca tcgccgacat tatcggtggt
    12841 aaacatcagg tttttcttga tttcttcgtc agtcaaaccg atgtctttca aacggttgaa
    12901 acgcataccg cttgccgagt ggcaggacag gcaatagttg gtaaagattt gtgcgccgcg
    12961 ctgcaggctg acttggtcgc gcaaatcgat atcgactttc tcatagtgtg catgaccgcc
    13021 cgaagcaaat gccgcaccca tgggtactgc caacagcaag gcagcaaacc aatttttcat
    13081 agcttgtttc attttgactg ccctcatcag atgttggttg caaacaggta agcacccacg
    13141 gcggtaatgc cgacgtaaac aaagaacatg attttttgtt tggcggtgct catggttacg
    13201 cgttcgggaa ccggtttgtt tttatccagc ttggtataga acggcatacc caggaagaat
    13261 gcgaaataga cgaaggacag gatgcgcgcg accaaagtac gcgtatcggt tgccaccatc
    13321 gcgcccaaaa tacccaaacc gataaaggaa atgatgaaca agaccaatgc ggttttgaaa
    13381 atcgggccgc ggtaacggac ggatttcacc tcgcccctat ccagccaagg cagcagggca
    13441 atcaacacga ccgccgcgcc cataccgatt acaccccaaa cctgcgttcc caagaacgag
    13501 ggaatcgcac gcaaaatggc gtagaacggt gtgaaatacc ataccggcgc aatgtgcgga
    13561 ggtgttttca gcgcatttgc cgcatcgaag ttcggcgctt cgaggaagta gccgccgcct
    13621 tccggtgcga agaacaatac ggcacagaag acaatcagga atacaaccac acccaagacg
    13681 tctttaacgg tatagtaagg atggaatggg ataccgtcgc gcgggatacc gttttcgtct
    13741 ttgtttttct tgatttcaac gccgtcaggg ttgttagaac ccacttcgtg cagggcgatg
    13801 atgtgcgcca caaccaagcc gagcaatacc agaggtaccg cgataacgtg cagggcaaag
    13861 aagcggttca gggtaacgtc ggaaacgttg aagtcgccgc gaatccaagt ggacaaatcc
    13921 ggaccgataa cagggatggc ggagaacagg ttaataatta cctgcgcacc ccaaaaggac
    13981 atctgacccc aaggcagcag gtagcccata aaggcttccg ccatcaatgc caagaaaatc
    14041 agagaaccga aaatccacac caactcacgg ggttttttgt acgaaccgta aatcaaacca
    14101 cggaacatgt gcagataaac aacaatgaag aagaaagatg cgccggtgga atgcatgtag
    14161 cggataatcc aaccgccgga cacgtcgcgc atgatgtact cgactgcagt aaaggcagca
    14221 ggcagatggt aggcgttcag gttgccgtcg ggtttgtagt tcatggtcag gaaaatgccg
    14281 ctgacgattt gaatcacgag gacaagcagg gccaaagagc cgaagtagta ccaaaaatta
    14341 aagtttttcg gtgcgtagta ttgagccaaa tgctcattcc acattttaga cagggggaaa
    14401 cgagcgtcca tccagcctaa caatgctttt gctttgctat tggtttggtt tgccataatt
    14461 atcgttcctt attcttagtc ttcgcccacc aagatagttg tgtcgctcaa gtatttatat
    14521 ggcgggacaa ccaggttggt cggggcagga acacctttat atacgcggcc ggccaagtcg
    14581 aatttcgaac cgtggcaggg gcagaagaag ccgcctttcc agtctgcacc caaatcggca
    14641 ggggcaatgt cgggacggaa ggtgggcgag cagcccaaat gggtgcagat accgatggcg
    14701 acaaggatgt tcggcttaat cgaacgggtc tcgtttttag catactccgg ctgctgttcc
    14761 gcatcggaat tgggatcggt aagttcgccg ttcaggcctt tcaggtcttt aagctgctga
    14821 tctgtacggt tgagcaccca aatcggtttg ccttgccact cggcggtcag caactgaccc
    14881 gcttcgattt tactgacatc cacctcgacg gcagcaccgg cggccttggc tttttccgaa
    14941 gggaaaaaac tggccacaaa cggcgttgcc acacccaatg ctgccactcc gcccgcgccg
    15001 caggtcgcga gtgtcaggaa acggcggcgg ccgttgttga tttcttgatt atccattatt
    15061 cagtcgtcct aatattttgg gaataccgag ccattaaacg ttgcaatttt acccagtttg
    15121 cagtgatact caaagcatta tttaaaataa ggtaaagttt tatgatattt ctcaagactc
    15181 aagccggatt gttttcgtca aaatggcaca cttccaaccc gaaaacctct gccgccgatt
    15241 ctgccagcgc gcgtacgccg taacgttccg tcgcgtgatg ccctgccgaa atgaaagccg
    15301 tacccgtttc attggcaagg tggtattgag cttcagagat ttcccccgtc aaatacagat
    15361 cgacaccttc gtctattgcc gtctgaaaaa acccctgcgc cccgccgcta caccatgcaa
    15421 cccgtcggat ttcgcgttcg ggtttgccga tgacgacagg cttacgttgc aaaactgttt
    15481 caatatgcgc cgccaatgcg ccgagtgtct tggcttgttt caggctgccc gagttgagca
    15541 ggttttgttc gccgaaccgt ttttctgtcg caaaacccaa tctgtcggcg agttgggcat
    15601 tgttgcccag tgtgggatgt gcatccaggg gcagatggta gcctgccata ttgatgtcgt
    15661 gccgtaacag tgcggcaatc cgttcttttt tccaaccagt aacggtcggc aactcgtttt
    15721 tccagaacat accgtgatgt accaaaagca aatctgcctt ctgctccaca gcaaaatcaa
    15781 tcgctgccct gcttgccgtt accgacgtaa cgattttccc gatatattcc ctcccttcaa
    15841 cctgcaaacc gttaggggcg taatctttaa acaacgctgt ctgcaatgtt tcattacacc
    15901 aagtcagaaa atccctgcgc aataccatct tttttcctaa tcgctttaaa caagcgggca
    15961 ttctaatcgc aaaatgtccg gaattcacat ttttccgatt tgcacccgca tatgaattat
    16021 tttaatatgc gccggttcaa tatgccgtct gaagccccat ggattccatt atcgaattgc
    16081 gccacctcaa aaccctgctg gcacttgaag aaaccggcag cgtctccctt gccgccaaac
    16141 gggttttcct tacccaatcc gccctttccc accagatccg tatgctcgaa aaccactacg
    16201 gcacgccgct gttcgaacgc aaatccacgc ccttgcgctt taccccggtg ggcgaaaggc
    16261 tgctgcgcct cgcccacgaa ctgatacctc aagttgctgt tgcagaacga gatttggcgc
    16321 gaatcacaga aggggaggcg ggagagctgc ggattgccgt cgaatgccat acctgtttcg
    16381 actggctgat gcccgccatg ggcgaattcc gcccgatgtg gccccaagtc gaattggata
    16441 tcgtatcggg attccaagcg gatcccgtcg gactgctgct gcaacaccgt gccgaccttg
    16501 ccattgtttc cgaagcggaa aaacaaaacg gtatttcttt ccaaccgctg tttgcctacg
    16561 aaatggtcgg catttgcgca ccagaccatc cgcttgccgc caaaaacgtt tggacggcgg
    16621 aagactttat cggggaaacc ctgattactt atcccgttcc cgacgagatg ctggatttgc
    16681 ccaaaaaaat cctgattccg aaaaacatca acccgccgcg ccgacacagc gaactgacca
    16741 tcgccattat ccaactggtt gccagcagac gtggcattgc cgcccttccc tattggacag
    16801 tcatgcccta ccttgaaaaa ggctatgtcg ttcaccggca gattaccgac gacggactgc
    16861 aaagcaaact gtatgctgcc atccgcactg aagatacgga caagagctat ctgaacaact
    16921 tttgccaaat catacgtgaa cgcggttttg cagatttgcc cggactgagc gaactggaat
    16981 cggtctgacc ccttattcaa ccatacccgg cagtttttct attttttcat gtatagtgga
    17041 ttaacaaaaa ccagtacagc gttgcctcgc cttgccatac tatttgtact gtctgcggct
    17101 tcgtcgcctt gtcctgattt ttgttaatcc actatactgt ttttgatttt tgcccaatct
    17161 gtaatcttta gattgccaat ggggaaccgt ctactacaaa taaaaaaccc tgcgataagc
    17221 agggtttttt gaatttccaa cattaacgtt tggagaattg ttttgcacgg cgtgctttgc
    17281 gcagacccgg ttttttacgt tcgacttcgc gggcatcgcg ggtaacaaaa ccagcttgag
    17341 acaaggcggg tttcaacgcg gcatcgaagt cgatcagggc acgggtaatg ccgtggcgga
    17401 ttgcgccgga ctggccggtt tcgccgccgc caacaacatt gactttgatg tcgaaagatt
    17461 cggcgttttc agtcagaacc aagggttggc gaacaaccat tcggctggtt tcccgtgcga
    17521 agaattcgtc aacgggacga ccgtttacga tgatttgacc tgtaccttta atcaggaata
    17581 cacgagccac tgaacttttg cggcggcctg tgccgtagta gtatttaccg ttcatgtcgc
    17641 gtccttattt cagttccaaa actttgggtt gttgcgcagc atgggcgtgt tccgcacccg
    17701 catacacttt cagtttttta atcatggcgt aacccagagg acctttgggc agcatacctt
    17761 ttacagcttg ttccaaagcg cggcccggga attgctcttg catttcgcgg aaggtgcgtt
    17821 cgtagatacc gcctgggaaa ccggaatggc ggaagtattt tttatcttcg aatttggcac
    17881 cggttacacg cagtttgtcc gcattgataa caatgatgta atcgccggta tcgacgtggg
    17941 gggtgtattc aggtttgtgt ttgccacgca gacggctggc gacttcggcc gcaacgcgac
    18001 ccaagacttt gtcttgggca tcgatgacga accattcgcg cttcacctcg tggggtttcg
    18061 ctgaaaaggt tttcatagtg gaaatccaga tagatataga aagttgtaaa ttttaaagac
    18121 aggattcgat tttgtcaatc gcattaccgc gttacggaag gattttccgg atttcggcag
    18181 actgcatact gctttttcgg gcggggcggc ggccaatgtg aaaaaccgca tcgttgcgat
    18241 gcggttttga atgggaatcc ccgcgagagc cgtttcggcc gaatccgctt gaaccttgct
    18301 gacaaggcgg ctgcctcggg tagtttcggg tgcgtccgca aaaggacgct cgcgcccact
    18361 actgctcccg gcaaccttaa gcgaacttat tggttcaaag gaatatatgc cttcgcggac
    18421 accgcaggga aaaaggggtt attcctgcgc caagcgggat agtgcttttt ggcaggcgtt
    18481 gtccatatcg gctattttac gcgcaaaatc gccgattgcc aaatcgccgc cgttcaggga
    18541 ggttttcaac aggtcgtgga cgacgttgag cgcggccata atgacgattt tttcgctgtc
    18601 cgcgacgcgt ccgccttcgc ggatggcttc ggctttgccg ttgagcattc cgactgcctg
    18661 caacagtgtg tctttttctt ctgccggcgt gttgacggtc agccgggcgt gcatgacttc
    18721 gatgtggact tgttcgatgt tcatccttta atccttattg ctgcgtttcc tgccgttggg
    18781 ggaggcgcgc tgccagtgcg ctgatttttt ccctgctctg ttcgagcagg ctgcggtatc
    18841 gtgtattttc ttctgtcagg ctgtcaattt tgttttgcag gtcttctttg agtttgccga
    18901 cttggacgag cagggcttcg ctaagttcgt cgacggcggt ttcgtgttcg agtttttgcc
    18961 gctcgtgcgc ccgtttgagt tcggcgacgg tttctttgag gcggcggttt tcgctgacga
    19021 gggtttcgaa tttttgtacc agcgtataaa cgctgctttc gagtttttcg atattttgtt
    19081 tcataacctt acctgtccgt atgccgtctg aaggcttcag acggcatctg tctgttgttt
    19141 attcaaaacg cgcgctacgt tccatcagcc tttcgacgac ctgctgaggg gtcatttcct
    19201 tacggatgag ttgcagcagg gtttgggtaa tcggcatatc gatttggtac ttacaggcag
    19261 tattgaagac ttcttctatc gtgctgaccc cttcggaaac gtgtccgatt tcgaccagca
    19321 cctgatgcag ttccttgcct tctgccaaac ccaagccgac gcggcggttg cgtgagagtg
    19381 cgccagtgca ggtcaagatg aggtcgccga tgcctgccag ccccatcatg gttttgggct
    19441 gtgcgcccat tgcggaggca aggcgggtaa tttcagctaa tccgcgcgta accagtgcgg
    19501 cacgggcgtt aagcccgtac tctaggccat cggacaatcc ggtggcaatc gccataacat
    19561 tttttaccgc gccgccaacc gccacgccga taacgtcggt actgccgtaa agcctcatga
    19621 cagtcgtgtt gagctgcggt acgagttctt caatccactc ttggttttcg gaggcgagga
    19681 cgacggcaca gggcagttgt ttggcgagtt cctgtgcaaa actcgggccg gagagtacgc
    19741 cgattttctt attgtcgggc aatacttctt tcaatacttg aaaggtcagc agccctgtat
    19801 cctgctcgaa tcctttgcag gcggcgagga cggggaggtg tcccgcgccg tactgtttga
    19861 gcagctctgc gctgcttctc aatccggcaa cggaggttac gataaggaca agtccgctgt
    19921 ctttgagcgc gtctgccaaa tccgcacaca cttccaaggt ttcgggaaag gaaaagccgg
    19981 gcagtccgcg tttgttttca cgcgcttcct gcatttggcg gacttggtct gcgttgcgcg
    20041 tccacaggga tacgcggttg ccgtgttggg aaaagtgcag ggcgagcgcc gtaccccacg
    20101 aacctgcgcc gataacggta attttcattg gtcgtctttc aacatatcac tgccgttcac
    20161 tttaaaacaa tcggtgtttc tctgcaagtg cggtcaggga aatgccgtct gaaaggtgtt
    20221 cagacggcat tttgccccga tgcggcacaa tcagcctgta ttgcgcaaac cttgcgccac
    20281 gccgttgatg gtcaggtgaa ccatcagcag cgcgtgcgga ttgtcgggtt ctttacgcag
    20341 gcgtttgagc atggcgacct gcaggccgtt gagcgcgttc aggtagggaa tcctcaaggc
    20401 aagcgaacgg gcgaggctgc ggttgtcgcg taaaagttct tcggtttgca gcaggtcgag
    20461 cagggctttg cggctgcgtt ggtattcttc cttaatcatg ccgaagatga cggcggcttt
    20521 ttccggcgat tcgctcaagc cggcatagtt ttccgcgagg gtgatgtcgg ttttcgccat
    20581 cacttgctcc atattggaaa gcatggcttg gaagaacggg ttgctttggg cgtgttcgcg
    20641 cagggcggcg agcgtttcgg gtttgtcttc gcacaaggtt tccaccgcgc tgccgaaacc
    20701 gtaccaagca ggcagcatga ggcggttttg catccaggaa aatacccacg gaatcgcgcg
    20761 caagtcttga atccgcgcta aggtcttgcg gctggcagga cggctgccga ggttgagggt
    20821 ggcgatttcc tgaatcgggc tggtttgcag gaagtagtcg atgaagtcgg ggtgggtaat
    20881 cagttcgcgg tagtatttga acgatacgtc cgacaatgcc tgcatcagtt tggcatcagg
    20941 gtctttttta tccggcagga tgctggcttc caaagtcgcg gcaaccaagg tttccaagtt
    21001 gcgttgggca ttgccggggt cggcgtattt ggcggtaatg acttcgcctt gttcggtaat
    21061 gcggatttgt cccgccacgc tgcccgccgg ttgggcgaga atggcttggt aagacgggcc
    21121 gccgccgcgt ccgacgctgc cgccgcgacc gtggaacagg cgcatgcgga catcgtattt
    21181 tttgaagagt tcgaccaagc ccaattccgc ctgatacaga caccatgagc tggtaacata
    21241 gccgccgtct ttgttggagt cggagtagcc gagcatgatt tcttggatgt ttccgcggct
    21301 ttcgagcagt gcgtcgtacc aatcgaggcg gaacatggtt tccatgaccg gacaggcgtt
    21361 ttcgagtgct tcgatggttt caaacagcgg cacgatattg atgcggctgt gcggtttgcc
    21421 gttttccacc gccaacaggc cgctttcttt cagcagcaat gccaaggcga gcaggtcgct
    21481 gggttgttcg cagttggaaa taatgctttg tgtaacggca tcttcgccga attcgtcttt
    21541 gattttgcgc gcttcgttga aaatcgccag ttcgcggcgg gtatggtcgc tgtatgtgat
    21601 aaacgggctg tacagcggac gttgatggct caattcgcgc aacagggcgg tttgtttttg
    21661 ctcttcgttc aggcggttgt agtcttccaa gcctgcgtgt tggaaaagct cggcaaccac
    21721 atcgacgtgt ttgcctgcgt gttggcgcag gtcgagcggc atcatgtgga agccgaacac
    21781 ggacacggag cggatgaggt ctgccaaacg gccttcagca agcaggcggc tgccgttgtc
    21841 gataagggaa cgttgcaatt ttttcaaatc atcgagaaat tcttgtgccg aagcatacgg
    21901 ctcgagaaag ccgaatttgc agcccatgcc caaaccgagc gcgcgcgctt tgcccatagc
    21961 gcgcgccatg atgtaagcaa tggcgcggcg gtagggttct tcggtgcgag cgatttcttc
    22021 gtcaggcgag agggctgcca gtgccattac gtcgtcgttg actttgacgc ggcggatgga
    22081 gagcggcagt tcgcggtaaa gtttgtcgag ttcgccacgg taaaaacgga acacggcatc
    22141 ggcgtggcgg cggaaggcaa aacgcaaggt ttcggcagaa acgaacggat tgccgtcgcg
    22201 gtcgccgcca atccagccac cgattttgag gatattcgga acgcagacat cgggatagac
    22261 catctgaaag tcgtgttcca tattgcggta gagcttgggc agggcttcga aaaagctcat
    22321 cgggaagatg gacacgccgt tgttgatttc gtcgttgacg ctgagtttgt ggcggcgcgt
    22381 ttcgctggtc tgccacaagc ccagcaggat agtgtcgatt tcgcggcgca gccgtgccag
    22441 cgcgtcggca ttggtgcagc gttcgcgttg cggcaacagt gcgcggatgc ggcggttgaa
    22501 gcttaagacg gtttggcgtt gcacttcggt cgggtgcgcg gtcaaaacgg cggtaacgga
    22561 cgtattgtcc aactgccgct gcaccgattt gccgtcggtt ttccccgctt tgagcctgcg
    22621 gacggtttcc gtcaggctgc cttccgcgcc gccgcgtccg gcttcttcgt ggatttggcg
    22681 gcggcgttcg tggtgcacgt cttcggcgat gttcaaaatc tgggcgaaca ggccgcaggc
    22741 caaggttaaa tcgtgggttt gttgttcgtc caattgcggc aatacttttt caatcaatgc
    22801 cgcgctgtcg tcggaagtgg acaagagttt gaccgtttcg acaaccaacg gcgaggcttc
    22861 ttcgtgcagg aggttgaaca gggattgttt cagaaattcc gcgtccgccg ccaaagccgc
    22921 gtccttcgga ttgttcagaa tatgcagttg catgattttt ctctcattgc cgtaaatact
    22981 gtaaatgtac ctcaaatgcc gcatccgtgc caaaccgttc acactttaac cgcccgtgtc
    23041 ccgaaatgcc gtctgaagtt gaacgccgcc cgacggcagc gttacaatcg cccgcaactg
    23101 ttttttccga acatcatcat gaccacgacc gaacacgaca acgacgacga actcctgctg
    23161 cggtacagcc gccacatcct cttggacgaa atcggcatcg aagggcagca aaagctttcc
    23221 gacgcgcata ttttggtcgt cggatgcggc ggtttgggtg ccgccgcact gccctacctt
    23281 gccgcttcgg gtgtcggcac gctgaccata gccgatttcg acacggtcga actgcacaac
    23341 ctgcaacgcc aagtcgcatt tgacgagggc gatgtcggca aactcaaaac cgaagccttg
    23401 gcaggccgcc tgaaacgcat caaccatacc gtcaacgtcc gcgccgtcaa cgaaaaactc
    23461 gacggctgcc gcctgaccgg tttggttcaa gccgccgaca tcgttttaga ctgttgcgac
    23521 aactacgcca cgcggcaagc cgtcaaccgt gcctgcgtgc aaacgaaaac accgctggtt
    23581 tcaggggcgg cggtacgctt tgaagggcaa cttgccgtgt accgacccga cttgcccgac
    23641 tcgccatgtt acgcctgcct gttcgacggc ggatcggctt cagacggcat ctgttccctt
    23701 ttcggcgtat tctcgccgct ggtcggcatc atcggctgca cgcaggcagc ggaggcactg
    23761 aaaatcctgc tggatgcagg cgaaccgtcg cacggcaggc tggcggttta ccgtgctttg
    23821 gaagggggct ggcgatattt cgacctgccg cgcaaccctg aatgcccggt ttgtggcgcg
    23881 gcacgataaa ccctgccgcc gtttcagacg gcataaaaac ggattcggcg gaacggtttt
    23941 aaaaatcggc ggtgttttga catagtgtcg ataagcatac ttatcttcaa tacactcaac
    24001 aggataattg tttgcctaag caaaaaaata atcgcctttt ccctgacggg cgggcgaaat
    24061 ccacggattg caaaaacaca acctgaaaat ttacatttct ttgcaaaaaa aaaaaaaaaa
    24121 aaataaactt accttataat tacggatgtc ttagcaatat ctgcttcgca gactcattga
    24181 gtaagatatt ttccccgtaa tgtgtttggc cgtctgtccc ctttgggttc ggatggcttt
    24241 tttttttgac tgtgtttgaa tacccggttg atttcgtctg tttgcagggg gaaagccgct
    24301 tatttccgtt cgggtggaaa acggttccat cggataaaag gcattttgtc cgactgatta
    24361 aagttacatc ttagagccgc atcggcggaa tattcaaaca cagctttttt aaggaaatcc
    24421 ggatacggcg gcgcatcaat aatgcggcgg aatctcgtcg cgcagggaat acggctcttg
    24481 cgcgtcggga ttcctgtcct gcattttttg atacagcagc ctcaactgag cctgttgcaa
    24541 atccagcgtc tgccgcaatt ccgccaccat cgcgttcagg ccggcgatta cgtcctcctg
    24601 aagcgcggat tggatttcca gctctgtgat gcggtgttcc aactcttgaa ccgcgtccat
    24661 ttacagcacc atcgcggcaa tccagccggc aatcagcagc gggatgttgt agtggatgaa
    24721 ggtcgggata acggaatcgc ggatgtggtc gtgctgcccg tcggcgttca gccccatcgt
    24781 cggacccagc gtggaatcgg acgcaggcga accggcatcg cccaacgccc ccgccgtgcc
    24841 gacaatggcg acggtggcaa gcggcgaaaa acccaaaccg acacacaaag gcacataaat
    24901 cgcggcaata atcggcaaag tggaaaagga cgaaccgatg cccatcgtta ccaaaagtcc
    24961 caccaccagc atcgccaacg ccgccatacc tttgctgttg ccgaatatcg ccatactgct
    25021 ttccaccagc ggctggatat gcccggtcgc attcatcacg gcggcaaaac cctgcgcggc
    25081 aatcataatg aagccgacca tcgccatcat cttgatacct tcgccgaata cgtcgtttgc
    25141 cttgtcgcgg ttgatgaccc ccaacatcat aaatacggcg aaaccgagca tcgcgcccaa
    25201 caccagcgag tcttcataca tcaactggat ggcaaaacat acggcaatgg cggcggcggc
    25261 ggccaggctg cggtaggcgg acggctgcgg acggtttgcc gcatcggcgt tgcccgccgt
    25321 atcggcattg ttgctttggt acaggcgcgg tttgcggtaa tggacaaacg ccagcaggag
    25381 tccggtcagc attcccaacg cgggaatcgc cattgccgcc attacgttga tgtttttcac
    25441 atcaagctgc ggcgcggcgg aatggatgtt gcccaacagg atttcgttca aaaaaatcgc
    25501 gccgaagccg taaggcagga acatataggt cgtaaccagc ccgaaagtaa tgacgcacgc
    25561 aatcagacgg cggtcgattt tcaggcggtt gaacaccaaa agcagcggcg gaacaatcat
    25621 cgggataaag gcgatgtgga taggtatgat gttctgactc ataatgccca tcacaaggat
    25681 gatggaaagc agcagccatt tgaccgcgcc ctcgcccgaa cgcacgctgt cgggcatacc
    25741 gccccggttc agcttgcgga cgaccgcgcc ggcaagctgc tgcggcaggc cggaatgggt
    25801 aatcgccatt gcaaacgcgc cgagcatcgc ataagaaagc gcaatcttcg caccgccttc
    25861 caaacctttg ttgaacacgg ggataatccc cgcctgactg acctgtcccg ccgcatcggc
    25921 aatgttttgc agcggcatac ccgccaccgc gccgccgaca aacgcgccga ccgtcaggct
    25981 caataccacg tgcacgcgcg acagcgacag caccagcata acgattacgg caactacgac
    26041 tgcattcatt gtttatcgct ccaaacctat aaatgtttac atatcgaaac acatcataac
    26101 ccaataatgg aaaacccgcc aattttgcaa acaattattt caaatgcttc atatacttcc
    26161 ccagcgtaac cctgtccaaa cccgccaagt ccggcagggt ttccactccc gaaaaaccat
    26221 tctccgccaa cacgccgcgc accgccgcgc cctgatcgaa accgtgttcc agcaataaaa
    26281 aaccgccttc cgccaaacgg tcgggcgcgc cttgcgccaa ggtgcggatg cagcttaggc
    26341 cgtctgaaaa gtcggtcagc gcgatttgcg gctcaaaccg caaatcgcct tgcaacaaat
    26401 gtttatcgcc gttttcgata tagggcgggt tggacacgat gatgtcccat ttcccttcag
    26461 acggcatatc ggtgtcgaac cacgaaccgt gtgcaaattc gacccgcgcg cccaaatctg
    26521 ccgcattttt ccgcgccgtt tcaagggcgg gcgtgctgat gtcggatgcg cgcacaaacg
    26581 catcggggcg ttcgagtgcg acggttacgg caaccgcgcc gctgcccgtc cccaaatccc
    26641 acacgcgccc gttttcgggc aggcgcgcca atacggcttc gaccaagtgt tcggtttcgg
    26701 ggcgcggaat cagcacgctc ggattgactg taaagcgtct gccataaaat tcgcgcacac
    26761 ctaaaatata ggcaaccggc tcgccgttca gacggcgttg cgccagcctg tccgcccgct
    26821 gtcggacttc gtccggcatt tcttccccgc cccgcgtcaa caactgcacg cgcgtatatt
    26881 ccgaaacata ttgcagcagc attcttgctt caattttagg cagttttgac aagcccaacc
    26941 atttatcaaa cgtcattttt atcccgtctg ccgctgatgc ggcttttctt tccttattct
    27001 ttccggcaaa cgtaccgatg gtggcaaccg caaatgcggc ataccacaaa taaaatcctg
    27061 caccgtagcg cacaatatcc gatgtattcc ctgcttcatc gacgtatacg gctttcacac
    27121 tgaaagccac caacgccaag ccccaaagtg ccgcatggac aggcacgacc ttcttccgca
    27181 acgccagcaa aacaatggcc gccaaccaaa cataattcgc atagaccgca caatacctga
    27241 tatccaaaga agcaaatatc gaccccaaaa tcaaaacggt caaaccctcc atgcttccat
    27301 gattgcccaa ataaaatgca acattggata aagacgctat ccacagggca accgacacca
    27361 gcaacatcac tatgggaaaa cttgatttcc gattctgttc ctgcatggtt ttatcc
//
LOCUS       DAWKOD010000010        26142 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000010 DAWKOD010000000
VERSION     DAWKOD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.010"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            660..2108
                     /gene="gnd"
                     /locus_tag="WJK73_000315"
     CDS             660..2108
                     /gene="gnd"
                     /locus_tag="WJK73_000315"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ7556855.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2171..3442
                     /gene="waaA"
                     /locus_tag="WJK73_000316"
     CDS             2171..3442
                     /gene="waaA"
                     /locus_tag="WJK73_000316"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ7556856.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TVKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGASARIAEAVREAVCGHRGR"
     gene            3483..3932
                     /locus_tag="WJK73_000317"
     CDS             3483..3932
                     /locus_tag="WJK73_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ7556857.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3965..4939
                     /locus_tag="WJK73_000318"
     CDS             3965..4939
                     /locus_tag="WJK73_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7556858.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            5008..5083
                     /locus_tag="WJK73_000319"
     tRNA            5008..5083
                     /locus_tag="WJK73_000319"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5041..5043,aa:Lys,seq:ttt)
     gene            5303..6556
                     /gene="murA"
                     /locus_tag="WJK73_000320"
     CDS             5303..6556
                     /gene="murA"
                     /locus_tag="WJK73_000320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ7556859.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6637..7815
                     /locus_tag="WJK73_000321"
     CDS             6637..7815
                     /locus_tag="WJK73_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ7556860.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCLDNGASVIVMTHLGRPTEGEFHSEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(7883..8131)
                     /locus_tag="WJK73_000322"
     CDS             complement(7883..8131)
                     /locus_tag="WJK73_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7556861.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(8222..9139)
                     /gene="ftsX"
                     /locus_tag="WJK73_000323"
     CDS             complement(8222..9139)
                     /gene="ftsX"
                     /locus_tag="WJK73_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ7556862.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLVRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(9136..9786)
                     /gene="ftsE"
                     /locus_tag="WJK73_000324"
     CDS             complement(9136..9786)
                     /gene="ftsE"
                     /locus_tag="WJK73_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ7556863.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9981..10463)
                     /locus_tag="WJK73_000325"
     CDS             complement(9981..10463)
                     /locus_tag="WJK73_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7556864.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10587..10940
                     /gene="arsC"
                     /locus_tag="WJK73_000326"
     CDS             10587..10940
                     /gene="arsC"
                     /locus_tag="WJK73_000326"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ7556865.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            10937..11593
                     /locus_tag="WJK73_000327"
     CDS             10937..11593
                     /locus_tag="WJK73_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ7556866.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11709..13103
                     /locus_tag="WJK73_000328"
     CDS             11709..13103
                     /locus_tag="WJK73_000328"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ7556867.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13124..13525
                     /locus_tag="WJK73_000329"
     CDS             13124..13525
                     /locus_tag="WJK73_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556868.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGERRRRGIDR"
     gene            13522..14013
                     /locus_tag="WJK73_000330"
     CDS             13522..14013
                     /locus_tag="WJK73_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7556869.1"
                     /translation="MNSLFVDNTVFITRLKAVHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDGELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGQHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14137..16731)
                     /gene="mutS"
                     /locus_tag="WJK73_000331"
     CDS             complement(14137..16731)
                     /gene="mutS"
                     /locus_tag="WJK73_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ7556870.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTTLPQLQKAAKAAAALDVLSTFSALAKERNFARPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPFDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17173..18177
                     /gene="gap"
                     /locus_tag="WJK73_000332"
     CDS             17173..18177
                     /gene="gap"
                     /locus_tag="WJK73_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7556871.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18247..18366)
                     /locus_tag="WJK73_000333"
                     /pseudo
     CDS             complement(18247..18366)
                     /locus_tag="WJK73_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18435..19115)
                     /gene="tsaA"
                     /locus_tag="WJK73_000334"
     CDS             complement(18435..19115)
                     /gene="tsaA"
                     /locus_tag="WJK73_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ7556872.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            19230..19865
                     /locus_tag="WJK73_000335"
     CDS             19230..19865
                     /locus_tag="WJK73_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ7556873.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            19902..20870
                     /gene="waaC"
                     /locus_tag="WJK73_000336"
     CDS             19902..20870
                     /gene="waaC"
                     /locus_tag="WJK73_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ7556874.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21097..21846
                     /locus_tag="WJK73_000337"
     CDS             21097..21846
                     /locus_tag="WJK73_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ7556875.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21857..22792
                     /locus_tag="WJK73_000338"
     CDS             21857..22792
                     /locus_tag="WJK73_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ7556876.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEAIPAQNLSRFVNRKLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            22855..23466
                     /locus_tag="WJK73_000339"
     CDS             22855..23466
                     /locus_tag="WJK73_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ7556877.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIVFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(23518..23835)
                     /locus_tag="WJK73_000340"
     CDS             complement(23518..23835)
                     /locus_tag="WJK73_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ7556878.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            23993..25264
                     /gene="purD"
                     /locus_tag="WJK73_000341"
     CDS             23993..25264
                     /gene="purD"
                     /locus_tag="WJK73_000341"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ7556879.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25298..25900
                     /locus_tag="WJK73_000342"
     CDS             25298..25900
                     /locus_tag="WJK73_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ7556880.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6262 a         6006 c         7368 g         6506 t
ORIGIN      
        1 ctttcatggg aatgacggga tgtaggttcg tgggaatgac gcggtacagg tttccgttac
       61 gtatccccat ccgaaagttt atttatacac aagcaatcca aagttatcag actgaaatat
      121 tgtgagattt acccgcaaat ctcatttttt ccaccactta tacacaaatt cggatgcgca
      181 ctctgccgat atgcagaaat aagacattcg tgccgacacc tgaccatttc cacaagttcc
      241 aaacacgctt ccggcttaaa tctccacagt attcccgttc ttaaaggcgc cagcagccat
      301 tcggtaaaga aaagtaggat gccatctgca caacaagcaa actatccaca aaacttattc
      361 cccataaaaa acaatccaca ggtatgcaca aaagggaatc cgccttacct acaccctaaa
      421 aaaaggtaat cctgatgatt tttaaaataa aaaaaagtta cccacagaaa atttctccta
      481 tcaatataat catcgttttt ttatttaatt tttatatttt atttataagc cttcagggaa
      541 acgcacggta aagcgtaaaa gcatcttttc aaaaaattaa aaagttaagg attgttaaac
      601 aagtgtaaat caggtagagt tgcggtcgat aaattaaatc tataacggag cttcctgcaa
      661 tgaaaggcga tattggcgta atcggtttgg cggttatggg gcaaaacctg attttgaata
      721 tgaacgattg cggatttaag gttgtcgcct acaaccgaac aatcggtaag gtggacgaat
      781 ttttaaacgg cgcggctaag ggaacggata ttgtcggcgc gtattccctg caagacttgg
      841 tcgataagct ggcaaaaccg agaaaaatca tgatgatggt tcgcgcaggt tcggtcgtcg
      901 acgactttat cgaacaactg ctgcccctgt tggacgagga agacatcatt atcgacggcg
      961 gcaatgccaa ttaccccgac acgacacggc ggacacatta ccttgccgaa aaagggattt
     1021 tgtttgtcgg ggcgggcgta tccggcggcg aagaaggcgc gcggcacggc ccgtccatta
     1081 tgccgggcgg ggataagcgc gcttgggaag ccgtcaaacc gatttttcag gcgattgccg
     1141 ccaaaacgcc gcagggcgaa ccgtgttgcg actgggtcgg caaggacggc gcggggcatt
     1201 ttgtcaaaat ggtgcataac ggcatcgaat acggcgatat gcagttgatt tgcgaagcgt
     1261 accagtttat gaaagacggc ttggggctgt cttacgacga aatgcaccgc gtgtttgccg
     1321 agtggaacaa aaccgagctg gattcttatc tgattgaaat cacagcggca attttgggct
     1381 ataaggacga aggcggtgaa ccgctggtcg agaaaatcct cgatacggca gggcaaaaag
     1441 gcacgggcaa atggacgggc atcaatgcct tggatttggg tattccgttg acgctgattt
     1501 ccgaagcggt attcgcccgc tgcgtttcgt cgttcaaaga acagcgcgtg cagaccggca
     1561 agctgttcgc ccgaaccgtt acgcccgtcg aaggcggcaa acaagaatgg gttgaggcgt
     1621 tgagacaggc cctgcttgca tccaaaatca tttcctacgc acaaggcttt atgctgatcc
     1681 gcgaagcggg cgaaagctac ggctgggatt tggactacgg caacactgcg ctgctgtggc
     1741 gcgaggggtg catcattcgc agcgcatttt taagcaatat ccgcgatgcc tatgaaaaca
     1801 atcccgattt ggtgttcttg ggtgcggatc cgtatttcaa aaatattttg gaaaactgcc
     1861 tgccggcgtg gcgcaaggtg gttgccaagg cggtcgaatg cggcatccct atgccctgca
     1921 tggcttcggc gattacgttc ttggacggct acacgaccga acgtctgcct gccaacctct
     1981 tgcaggcaca gcgcgactac ttcggcgcgc acacttacga acgcaccgac aaaccgcgcg
     2041 gcgagttttt ccataccaac tggacgggca agggcggaga taccgcttcg acaacctacg
     2101 atatttgatt ccgttgccga aatgccgtcc gaaccgttcg gacggcattt gttttgcagg
     2161 aaatagaaag atgttccaat ggctttatga tgtattgtgg ctgcttgcgc cgatatggat
     2221 acggcgttat ttggacaaac gctccggaag tgccccggca tatcgggcgc atcgggacga
     2281 gcgtttcggc aagccgtatc cgaatcccgt taccggagcg gtttggatac acgccgtttc
     2341 ggtcggggaa acgcgtgccg cccaaccttt gatacgcgag ttgcggcagc gttttcccga
     2401 tgcgccgctg ctgatgacgc agatgacccc gacgggacgg gaaaccgcgc aagttctgtt
     2461 tcctgatgca caatgccgct atcttccgta tgacaaaaaa acgtgggtac ggcagttttt
     2521 gcgcgaacac cgcccgatgt tcggcatttt gatggaaacc gaaatctggc ccaatctgat
     2581 gaaggaatgc cggcgcacgg gtgtgccgct gtttttggcg aatgcgcggc tgtcggaaaa
     2641 atcgctaaac ggttatctga aagtccgcag cctgatccgt cctgccgccg cttcgctgac
     2701 gggctgtctg gcacagacgg aggcggatgc ggcgcggttg gcgaaattgg gcgcggcatc
     2761 cgtgcaggtg tgcggcaata ccaaatacga cctgatgccg tcggaacaga tgaaaacgct
     2821 ggcggggcag tttgaaaaac gcatcggaga ccggcaggtt gccgtgtgcg gcagcacgcg
     2881 cgtttatcgg ggtgaagacg aggcggaaaa actgctggcg gcgtggcaac aatatcgcgg
     2941 cgatgccctg ctggtcgtcg tgccgcgcca tcccgagcat tttcagacgg tatttgaaac
     3001 ggtaaaacgc ttcgggttta aggttcagcg gcgcagcgac ggtttgccgg tcgaacccga
     3061 tacgcaggtg tggataggcg acagtatggg cgagctgtat gcgtattacc tgtgcgccga
     3121 tgtcgctttt gtcggcggca gtctggtcga ttcgggctgt cagaacatca tcgaaccgct
     3181 ttcctgcggc gttccgacga tattcggctt ttcgacctac aatttttccg aagcctgccg
     3241 gcacgccttg gcatcgggtg cggcggttca agtcgaatcg gcggatgcgt ggcgtgaagc
     3301 cgttgaaaaa accttatcgt ccgagggggg cggaatgcag atgcaggcgc gcgtggacgg
     3361 ctttatcgca caacatcgcg gagcgagcgc gagaatcgcc gaggcggtgc gggaagcggt
     3421 atgcggacat cgggggcgat agtgatacaa tccgtatccg agttttccgg ttggagcatc
     3481 gtatgagtat ttatgccgtc gcgcacatcg tccacctgta ttgcgccatc gcctttgtcg
     3541 gcggcgtgtt ttttgaagtg ctggttttgt ccgtcctgca tacgggacgg gtgtcgtgcg
     3601 aggcgcggcg cgaagtggaa aaggcaatgt cttaccgcgc cgtcagggtg atgccgtttg
     3661 tggtcggact gctgttcgcc agcggcatcg tgatggcggc aaaccgctat ctttctatat
     3721 tgggcgaacc gtttgccact tccttcggta cgatgctgac gctgaaaatc ctgttggcgt
     3781 tcagcgtgtt ggcgcacttc gccatcgccg tcgtcaaaat ggcgcgttcc acactgaccg
     3841 tcggctggtc gaaatacata cacaccgtcg tctttaccca tatgctgctg attgtctttt
     3901 tggcaaaagc gatgttttat atcagctggt aacacaacta catttcaaat caggagattg
     3961 aaccatgaca gaatatcccg gcatcgggtc gccgttgttt tacggcgttt tttttgcggc
     4021 agtgctggtc atgattgcct tggatatgtt ttcgctgaag aaaaacagca gccataaagt
     4081 cggcgtcaaa gaagccttgg catggagcgg cttgtgggtt gccgtatcct gcctgttcgc
     4141 aggctggctg tatttcaaac tcgccggcaa tcccggctac ggcgcggcag tcgcaaaaga
     4201 aaaagtattg gaattcttta ccggctacat tttggaaaaa tcgttggcag tcgacaatat
     4261 tttcgtgttc ctgatgatat tcggctactt caaagtcgca ccgcagtttc agcaccgcgt
     4321 gctgctgtac ggcgtattgg gcgcattggt attgcgcact gtcatgattt tcgtcggcgc
     4381 ggcactggtt cagcagttcg agtggattct gtatctgttc ggcgcgttcc tgctctatac
     4441 tggtatacac atgatgaagc ccgaaggcga tgaggaaggg gatttggcaa acagcaggct
     4501 gctgaatgcc gtcaagaaag tcgttccggt cggcacggaa tttcacggcg agaaattttt
     4561 tactgtcgaa aacggtaaaa aaatcgccac gccgctgttt ttggtgctgg tcatgattga
     4621 attgagcgat gtcgtgtttg ccgtggacag catccccgcc gtctttgccg ttaccaccga
     4681 tccgtttatc gtgctgactt ccaatatttt cgctattttg ggcttgcggg cgatgtattt
     4741 cctgctggcg gatgtggcgg aacgctttat cttcctgaaa tacggcttgg cattcgtgtt
     4801 gggttttatc ggtgtgaaaa tgctggtaat gcattgggtg catatcccga tttccgtttc
     4861 gctgtcggtc gtgttcggcg cgttgggtgc atcgatactg acctcgttaa tttatacgaa
     4921 aaaacagtcc gataaataaa atgccgtatg agcgccgaca aaatggttga cagcatcagg
     4981 cttgatgtat agaatttcga gcttatcggg tcgttagctc agtcggtaga gcagcggact
     5041 tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataaaa caaaaaaagc
     5101 tgagttttca aaactcggct ttttttgtct tccgacaatc cttgttcgga aagcgcggca
     5161 tttcgtcaaa ttctttggtg cggcttccgt atttttcccg aaaatccctt ataattccgc
     5221 ctctgtaacc ttcatcgggt gatgctttat gcgttgtccc gagtattgaa atattacaag
     5281 tacccactag gaaagaacaa acgtggacaa actgaaaatc tccgcaaacg gcccgctcaa
     5341 cggggaaata acggtctcgg gcgcgaaaaa cgcggcattg ccgctgatgt gcgcgggttt
     5401 gctgacatcg ggtacgttgc gcctgaaaaa cgtccctatg ctggcagatg tgaaaaccac
     5461 acaaaagctg cttcagggga tgggcgcgcg cgtcctgacc gacaatatca gtgaatttga
     5521 aatcaacggt ggtacggtaa acaacacctg cgccccttac gaattggtcc ggacgatgcg
     5581 cgcctcgatt ttggtgcttg gcccgacgct ggcgcgtttc ggcgaggcgc aagtcagcct
     5641 gccgggcggc tgcgccatcg gttcgcgccc tgtcgaccag catttgaaag gcttagaagc
     5701 gatgggtgct gagattgtta tcgaacacgg ttacgtcaaa gccaagggta agcttaaagg
     5761 cacgcgtgtg gcaatggatg tcgttaccgt cggcggcacg gaaaacctgc tgatggcggc
     5821 gacgctggcg gaaggtacga cggttttgga aaactgcgcc atcgagcctg aagtggtcga
     5881 tttggcggaa tgcctggtca aaatgggcgc gaaaatcagc ggcatcggta cgtccacaat
     5941 gattgtggaa ggtgtggacg agctgcacgg ctgcgaacac agcgttgtcc ccgaccggat
     6001 tgaagcgggg acgttcctgt gtgcggtggc gataaccggc ggcagggtgg ttttgcggaa
     6061 tgctgcgccg aaaacgatgg aagtggtgtt ggacaaactg gttgaggcag gtgcggtgat
     6121 tgaggcgggc gacgattgga tcgccatcga tatgcggcag cgtccgaagg cggtggacat
     6181 ccgcacggtc gtccaccccg gcttccccac ggatatgcag gcgcagttta tggcattgaa
     6241 tgccgtggcg gagggaagct gccgcgtggt ggaaacgatt tttgaaaacc gctttatgca
     6301 cgtccccgag ttgaaccgga tgggggcgaa cattacaacc gagggcaata cggcatttgt
     6361 gcagggtgtg gaacggcttt ccggcgcagt cgtcaaggcg acggatttgc gtgcgtccgc
     6421 aagcctcgtt atcgccggtt tggcggcgcg cggcgaaacc gtggtcgaac agatttacca
     6481 cttggatcgc ggttatgaaa atattgaaaa aaaactcggc agcgtcggcg cgaaaatcga
     6541 gcgcgtatcc ggctgatcgg gttgcgatgc cgtctgaagc cttcagacgg catcaattgt
     6601 ttctgtatcg caaacactga ttgaaaagga ctgattatgg catttttaaa actgaccgaa
     6661 caaaacgtgc agggcaaaac cgtcctcatt cgcgccgata tgaacgtgcc gttcaaagac
     6721 ggcaaaatca gcgacgacac ccgtatccgc gcctcacttg cgtccatcaa atactgcttg
     6781 gacaacggcg cgtccgtcat cgtgatgacc cacttgggtc gcccgaccga aggcgaattc
     6841 cattccgaag acgatgtcgc gcccgttgcc gcgcacttgg gcggtctgtt gggcaaagac
     6901 gtgaaagtat tgaacgactg gcgtgaaaac aaaccggctt tgaacgcggg cgatgtcgtg
     6961 atgctgcaaa acgtgcgtat caacaaaggc gagaagaaaa acgatttgga actgggcaaa
     7021 gcctacgctt ctttgtgcga cgtgttcgtc aatgacgcgt tcggcaccgc gcaccgcgcc
     7081 caagcctcga ccgaagccgt cgcccaagcc gctcccgttg cctgcgccgg cgtattgatg
     7141 gcgggcgaac tcgacgcttt gggcaaagcc ttgaaacagc ccgcgcgccc gatggttgcc
     7201 atcgttgccg gcagcaaagt gtccaccaag ctgaccatcc tcgaatcgct ggcggacaaa
     7261 gtcgaccaac tcatcgtcgg cggcggtatc gccaacacct tcctgttggc ggaaggtaaa
     7321 gccatcggca aatctttggc ggaacacgat ttggtggaag aatccaaaaa aattatggcg
     7381 aaaatggcgg ccaaaggagg ttctgtgccg ctgccgaccg atgtcgtcgt tgccaaagcc
     7441 tttgccgccg atgccgaagc ggtggtaaaa gacattgccg acgttgccga agacgagatg
     7501 attttggaca tcggcccgaa atctgccgcc gcgcttgccg atttgcttaa agccgccgac
     7561 acggtcgttt ggaacggacc ggtcggcgtg ttcgagtttg accagttcgc aggcggtacg
     7621 aaagcccttg ccgaagccat tgcccaaagc aaagcgttct ccattgctgg cggcggcgac
     7681 acgctggcgg cgattgccaa attcggcgtt accgaccaaa tcggctacat ctccaccgga
     7741 ggcggcgcgt tccttgagtt cttggaaggt aaagagttgc ctgccgtagc cgctttggaa
     7801 aaacgcggcg agtaaacggt ttgatataaa aaaatgccgt ctgaaacgtt gtgctgtttc
     7861 agacggcatt tttgtgtggc ggttattgtg ctttggcatt ccattctgcc ggtgtggcgg
     7921 ctaccgaaat ggaaagtgcg tgcagttcgt tgctttccag ttgggcttta agcccgtctt
     7981 taatcaggcg gtggcgcgcg aggcgtgcct tgccttcaaa ttctgatgaa acgatgacgg
     8041 cgaaaaaatg atgtccgtcg ccttctactt cgatgtgttc gcattttgcc acgccttcaa
     8101 tcatggcttt tacttgttcc gaggtcagca tggtaggttc cttgttggaa aaatgcgcca
     8161 ttatactggc aaattgaaat gccgtctgaa aacgggttcg gacggcattt ttgacggtgt
     8221 tttatttttt ggctttgaag ccgagcaggt gctgggtggt ggcaagccac gcgccgaata
     8281 tccccagtgc gatgacgaag ccgaacacca gtgcgatttc tcccgggtag aagaaacgcc
     8341 agccgatatt aagtccgtag ggtttgaaga tggcatcgac caaggggcgc acggcagaga
     8401 gcagccaacc gcaaagcccc aagctgacgg cggcggaaag gatgctctgc cacatggctt
     8461 gataaaggaa tgggcggcgg ataaacgacg cgggcgcgcc caagagtttg gtgatttcga
     8521 tttcttcttt tcggctgagg atttgcagtc ggatggtgtt gtgcgcgaca aggacgaacg
     8581 ccatccccag cgtcagggaa agaaaccata agattttacg gatgaattcg ttgatttggt
     8641 acagtgtctg tacccattcc gtatccatgt tggcggattc gaccataggc agtttggtaa
     8701 tgtcccggta gattgcctgc atttgggcgg gcgtggttgc cgggtcgggg gtaacgataa
     8761 agacatccgg cagggggtta ccgtcaagca tggaaatcag attttggtcg agattggact
     8821 gtaattcttc caaaccgtct tctttgctga taaagcggat gttgtcgagc cgtttgtcgc
     8881 gcaccagcag gctgcggacg gtatcgctgt cgctttgtgc ggcggcggtt tccatataga
     8941 cggtgatttg cggcgactca ttgagtttgc ccaacacgct ttgcccgctt tggatgccca
     9001 agtacataaa cagcggcagg gtcatggcga cggcgagcat tatgagggta agcagtgtgc
     9061 cgaaaggttg gcgcagaagc tgtttgagcg cggtgcgcgc ggattcgacg tgcagcgaga
     9121 ggtagtggat gatgctcatg cgagtcgtcc tttcgagagg cgcaggatgc ggtgtccgta
     9181 gtccgccatc agggtttcgt catgtgcggc gacgatgacg gttgttcccg cttcgtggaa
     9241 ggttttgaac aattccataa tatcgagcgc gtaggcgcgg tcgaggttgg cggagggttc
     9301 gtcggcaatc agcaggctgg gctggtgaac gacggcgcgg gcgatgcaca ggcgttgttg
     9361 ttcaccgccg gagagggtta cgggatcgtc caattctcgt cctttcaggc cgactttttc
     9421 gatggcgatg cgggcacgct cttcggcttt gcgcggcgga tagccgataa tccgaagcgg
     9481 caggatgacg ttttgcagga cgttgcggtc gtagaggatt ttgtggtctt ggaacacgat
     9541 gccgatgtgt tggcgcataa agccgatttg gttgtcggac aatgtgccga gatcctgccc
     9601 gttaaacagg attttgcccc tgctcggctt ggtgatgccc gaaatcagct tgaggacggt
     9661 ggatttgccc gaaccggagt gtcccgcgat aaatatcatt tcgcctttgt tgatttggaa
     9721 gctgacgttt ttcagggctt caaaaccgcc gggataggtt ttggaaactt gttcgaaacg
     9781 gatcatagga ggtcctgtaa ggaagggcgg catatcgttt cgggcggttg cgtccgcagg
     9841 gatatgccgt ctgaacaata ggtaagacag tgccgattat aaagattccg cctgttttta
     9901 gatagtggat aagtttggcg gataccgaag gtcgggcggg aaaaatgccg tctgaagcgg
     9961 cttcagacgg catccggcgt ttaacggcat tttgaatggg cgagtttgac ggcttcggtc
    10021 aggctttttt cgttcacttc cccggtaatg gtttgtttgt acccgcattt cggggcttcg
    10081 acgacggtaa agggcagtac gccgacattg tttccgtagg atttcataaa gtttcggctg
    10141 ttcgcctcgg tgtaacgcca aatcgggtag gaaacaggag tttgtttgag gaagttgccg
    10201 atattgtcgg atgtgtcgag cgcgatgccg accatatcga cgctgccttt tttctgcgct
    10261 ttgtaccatt tggacatggc gggcatctct tttcggcacg ggccgcacca ggttgcccaa
    10321 aggttgacga tgcgtacggg ggctttgagt gattgcaggc tttgtggggt gttgtctttc
    10381 catccggcca gttcgtctgc cgaggcggta tgtgcggcaa atgtggcggc aagggtgatt
    10441 gcggcaagat tcaagtattt catcgttttg tcctttgtat gtcgattcgg gtattgtaaa
    10501 ccgtttggta cggttgtagg cgggatattg agtaaaaccc tgtgtaagcc gataataagg
    10561 ggattgtcga gaagcaagga aatgtcatgc ctgaaatcaa aattttccac aatccgcgtt
    10621 gcagcaaatc gcgtgccgcc ttgtccctat tggaagaacg cggcattgct gccgaagtgg
    10681 tcaaatattt ggatacgccg cccgacttgt ccgaattgaa ggatattttc aacaaattgg
    10741 gcttggcatc ggcgcgcggg atgatgcgcg tgaaagatga tttgtataag gaattgggtt
    10801 tggacaaccc cgatttggac aatgacgcgc tgctgcgtgc catcgccgag catcccgccc
    10861 tgttggagcg tccgattgtt ttggcgaacg gtaaggcggc tgtcggcaga ccgctggaaa
    10921 atatcgaagc gatattgtga acaaacggct gttttgcagc cgcaacggct tgcgctatta
    10981 cctgttgggc ggtttctgtt tgagtgtttt tccgcttttg ctggtgtttg cctcatccgt
    11041 ttgggcggtt tatcggacgg gcgggcaggt tttgccgccg tatgtccggg cggatgccgc
    11101 gctggtgttg ggtgccgccg catgggacaa acgcccttcc ccggtttttc gggaacgcat
    11161 caaccacgcc atcgcgcttt accaaagccg cagggtaggc aaaatcgtgt ttacgggagg
    11221 ccggaccaaa aaaggctata tgaccgaagc cgaggtcggg cggcggtacg cgctcaaaca
    11281 gggggttccg gcgcgcaaca tcctgtttga aaacacttcg cgcaacactt atgaaaacct
    11341 gaacaacatc cgtccggttt tgcgtgccaa cggtattgcc agtgtggtga ttgtcagcga
    11401 tccctaccat ttggcgcggg cggcggaaat ggcggaggat ttgggtgtcc gcgtttatat
    11461 gtctgccacg ccgaccacgc gctttgatgc gggaaataag aaaaaaatct ttatgctgca
    11521 agagggatat gcgttatccc tctaccgttt ggaaaaatgg ggcagccgct ttttgggatg
    11581 gctgtctgat tgaagcggta gaaaatgccg tctgaaactt atccgcaggc agggttcgga
    11641 cggcattttt aatgcccaag cacggtaaaa acagtaaaat aagccgtttt aaagattctg
    11701 gagccatcat gaccgtcaaa acccgtttcg cccccagccc taccggctac ctgcacatcg
    11761 gcggcgttcg caccgccttg ttttcgtggg cgtttgcccg ccatcataaa ggcgaatttt
    11821 tattgcgtat cgaagacacc gacttggcgc gttccaccgc cgaatctgtc aatatcatcc
    11881 ttgacggtat ggaatgggtc ggtctcgatt acgacaacgc cgacaacgtg gtgtaccaaa
    11941 cccgccgctt cgaccgttat aaagaagtca tcgccgaact cttggcaaaa ggcgatgcct
    12001 actactgcta ttgcagcaaa gaagagctgg aagcgatgcg cgagaaagcc gaaaaagaag
    12061 gcacggcgac ttacgaccgc cgctggcggc cggaagcagg caaaaccctg cccgaaatcc
    12121 ctgccggcgt gcaacccgtc gtccgcttca aaacgccttt ggatggcgta accaagtgga
    12181 cggacttggt caaaggcgaa atctccatcc ccaacgaagc actcgacgac ctgattatcg
    12241 cccgcgccga cggcacgccg acctacaact tctgcgttgt cgtggacgac tacgacatgg
    12301 gcgttaccca cgttatccgg ggcgacgacc atgtgaacaa caccccgaaa caaatcaaca
    12361 tcttaaaagc catcggcgct accctgcctg aatacggcca cctgccgatg attcttaacg
    12421 aacaaggcaa aaaaatctcc aaacgcagcg gcgataccgt tgccattacc gatttcggcg
    12481 cgatgggcat cctgcccgaa gccatgctca actatctggc acgtttgggc tgggcgcacg
    12541 gcgacgatga gttcttcacg atggaacaat ttatcgaatg gtttgatttg aaagacgttt
    12601 ctccatctcc aagccgcatg gatttgaaga aactctactg gattaacggc gaacacatca
    12661 aaatcacgcc caacggcaaa ctcgccgaac tcgtcaaacc gcgtctcgct ctgcgcgata
    12721 tccatgaaac tgaaaaacct gcattggaag acgtgttggc actggtcaaa gaccgcgccc
    12781 aagacctgaa cacacttgcc gacgagtgcc tctacttcta cgtcaaacaa acgcctactg
    12841 aagcggacgt gcagaaacac tgggacgatg aagccgccgc ccgtatgctg cgctttgccg
    12901 aacgcctcga agggctggaa gactggaatg ccgaagccat ccacgacctc ttcaaaccct
    12961 tctgcgacga agaaggcatt aaaatgggca aactcggcat gcccctgcgc cttgccgtct
    13021 gcggtacggc gaaaaccccc agcgtcgatg ctgtgttggc attaatcggc aaagaagaag
    13081 tgttgaaacg catccgcgct taagttatcc acaggttcgg gtaatgccgt ctgatgtcgg
    13141 aatgcggctt cagacggcat tcaaacggaa gttgaacatg aaaaaaatat tgtattttct
    13201 gatgtttgtt ttttctacaa gcgtatgggc aggggatgct gaagacaatt tgctcagtat
    13261 ccaatccggt taccgtgcct tattgcaaaa gcaaaacaat ctggacggaa aaatcatcgg
    13321 gatgcagtcg gatttggaag atgcgcgccg gcgtttgcag gcggctcagg cagacatcgc
    13381 ccgtttggaa gcggaaattc ctgcagcaat ggcgcaaaaa gcccggcagg ctgaagagct
    13441 gaggcaaatc ggagtgcgtt tggaccatgc ttggaatgcg gtttacggcg caggggaacg
    13501 aaggcgtcgg ggaattgacc gatgaattcc ctctttgtag acaatactgt tttcattaca
    13561 cggctgaaag ccgtgcatat cggcaggttg gttcaggcgt tgtttgagga gtggcacgga
    13621 tttgaaccgt ggtcttctgt ggataagatt catgcctatt acggcaggtg tttgaaggat
    13681 ggcgaactgc cactggcatt tgcggctgtg gatgactccg gaatcctgtt gggttcggct
    13741 acggtcaagc ggcatgatat ggaaagtttt ccacagtatg aatattggtt gggtgatgtc
    13801 tttgttttac ctgaatatcg cggaaaaggc attggcagga ggctggtcgc ccactgcata
    13861 ggcgcagcgc gttcgctggg gataaagttc ttgtatcttt atacgcctga tgtgcaaata
    13921 ttttatgaat cattcggctg ggtggttgtc gggcaacatt tccataacgg tgaatgggtt
    13981 acggttatgc gtttggatgt ggataaggtt taagccgaat ggacagatgc cgtctgagct
    14041 gttcggacgg cattttttat atttggaaat ttcctgtcag ataatgttta tccacagatt
    14101 tttccatatg gtttgatgct gttccgacaa cggaaattaa gatacggatt tgcacaaatc
    14161 tttcagacgg tacagttctg acaatgcttc gcgcggggtc aggctgtcgg gatcgagttt
    14221 ctccagggct gctgccaata taccttcaaa atgtttttcc tctgctttgt ccacaaagct
    14281 gtccacattc ggttcatctc ctttttcaga cggcatggta ctgaaaatat ccagttgggg
    14341 acggttcgcg gcggcttggt tttccagttc gttcaaatgc ttttgggcgg atttcaatgc
    14401 gcgtacaggc aggccggcga gtttggcgac ggcaatgccg tagcttttac cggcgggacc
    14461 cggttggatt tggtgcagga aaacgatgtc ctgtccctgt tcgagcgcgg aaaggtgcat
    14521 attgacggcg gcggcgtggg cttcgggcag gtaggtcagc tcgaaatagt gggtggcaaa
    14581 caggctgaag gatttgtttt tttgcagcag gtgttcagca acggcgtgcg cgagggcgag
    14641 gccgtcgaaa gtggaagtac cacgtccgac ttcgtccatt aaaacaaggc tttgttcggt
    14701 ggcgtgatgc aggatgtagg cggtttcgct catttcgacc atgaaagtgg agcggttgga
    14761 ggcgaggtcg tccgatgcgc cgatgcgggt gaagatttga tcgaagggcc cgattgtggc
    14821 ggcatcggca ggcacaaaac agccggtgtg tgccaataaa acaatcagcg cgacttggcg
    14881 catgtaggtg gatttgccgc ccatattggg gccggtgagc agcatgaggc ggtgtttgtg
    14941 gtcaaggtcg gtgtggttgg cggtgaagtg gcgtacctgc tgttcgacaa cgggatggcg
    15001 gccgttttcg atgtggataa ccggatagtc ggcaaactcg gggcgggcga agttccgctc
    15061 ttttgccaag gctgaaaatg tggacaatac gtccagcgcg gcggcggctt tggcggcttt
    15121 ttgaagctgc ggcaatgtcg tctgaaggtt tttcaatacg ccgtcaaaga gttgtttttc
    15181 taaggcgagg gcttgctctt gagcagtcag cactttgtct tcaaaggctt tcaactccgg
    15241 cgtgatgaag cgttcggcgt ttttgagggt ttgccggcgt tggtagtcgg caggtgcttg
    15301 ttcggcttgg gttttggaca attcaatgta aaagccgtga acgcggttga actcgacttt
    15361 gagtgtggac aaaccggtac gttcgcgttc cttggcttcc aaatccagca aaaattcgtc
    15421 gccatggttt tgaatgcggc gcaattcgtc cagttcggga tgaaaaccgt ggttgatgac
    15481 attgccgtct ttcagccaga cggaaggttc gggcaaaatg gcttggcgga gctgttcggc
    15541 tgtggatagg ttttccggga aaacggcttt gagggtttcc aacaggctgc tgccgtttgc
    15601 ggacaagtcg atttgtgcca actcgaacag actgtcgcgt aaagatgcca aatcgcgcgg
    15661 gcgggcgttg ccgacggcga tgcgggcagc aatgcgttca atgtccgcaa tgtttttcag
    15721 acggcattgg aggggtttgt attggctttc cagcgcggca acggcttctt ggcgcgctcg
    15781 gatgtgggcg cggttgcgta aagggtggtg cagccagaga gccaagaggc ggctgcccat
    15841 atgggtagcg caaaggtcga gcgtggacat cagggtcggc gattttttgc cggagagggt
    15901 ttgcgtgatt tcgagattgc ggcgcgtggc ggcgtccatg ccgatatatt ggctgtcggt
    15961 ttcgagcgac aggccgtcca aatgttgcgg catcaggttt tgcgtcaggc ggatatagtt
    16021 caacagcgca cctgccgcgc caatcgcaac ggcgtgttct ttgccgtcca aaccgaagcc
    16081 gcgcaaatcc tggcagccga aatattccgt cagcagtttt tcccccgcgt cggcggcaaa
    16141 ctgccacgcg ttcaggcgcg taacacccga tgccgtctga agttgcggtg cgtttttact
    16201 gtccggcaac agaatttccg ccgcctgcag gcgcgccagt tcgtcgtcca atttatccac
    16261 agttgtcagc ttggttttga attcgccgct ttgcagcgat gcccacgcca aaccgatgta
    16321 ttttttgtcg ggggacacgg caacgatacg gttggtttcc ttgtcttcca gcaatgcgga
    16381 atcggtcagc gtgccgggcg ttacgatgcg cacgactttg cgctccacag gccctttgcc
    16441 cgcgccgact tcgccgacct gttcgcaaat cgccacgctt ttgcccaact tgaccaggcg
    16501 cgccagatat tgttcggcgg cgtgaaacgg cacgcctgcc attttgaccg gctcgccatc
    16561 cacctgtccg cgcgtggtca gggtaatatc caaaagtttt gccgcttcta ccgcatcgtc
    16621 gaaaaacatc tcgtaaaaat cgcccatacg gtaaaacacc agtttgtcgg tatgttgcgc
    16681 tttgatgccg aggtattgct gcatcattgg ggaaacggcg gatttggaca tggggctgcc
    16741 ttatcattag gaaaacggta ttgtagcaaa aggttttgtc ttcgacggaa tgagactgac
    16801 cgtaagcctg tcaataaggt tgtggagaca actaggaaaa cagcaaagtt atccacagaa
    16861 atattattaa gtcaaaaaac atccacgctt atctgtctta tacagcaagt cttaaactca
    16921 aacttatttg acaaaaagat aatgaaaata ttggatattt gctacttacg cacaagcagg
    16981 ttggcaatca tcaaaatcat cattaaaatt taaattcaaa tattgatggt aaaggttttg
    17041 gaaatctgtg caaacagcaa aattcatcta tatcaaataa gcgtcaagta gttagatgta
    17101 atagtggaat gtaactttat ttcagaatac aatgcaggca tcggacttta tttcacaacc
    17161 tggagacaaa atatgagcat taaagtagcg attaacggtt tcggccgcat cggccgcctc
    17221 gcattgcgtc aaattgaaaa agcccacgac atcgaagtcg tcgccgtcaa cgacctgacc
    17281 cctgccgaaa tgctgctgca ccttttcaaa tacgacagca cgcaaggccg tttccaaggt
    17341 actgccgaat taaaagacga tgccatcgtg gtaaacggca aagaaatcaa agtttttgcc
    17401 aatccgaatc ccgaagaatt gccttggggc gagttgggtg tggatgtcgt cctcgaatgt
    17461 accggcttct ttaccaacaa aaccaaagcc gaagcccaca tccgtgccgg cgcgcgcaaa
    17521 gttgtgattt ccgcacctgg cggcaatgat gtgaaaaccg tcgtatatgg cgtaaaccaa
    17581 gacattttgg acggcagcga aaccgtcatt tccgccgctt cctgcaccac caactgcctt
    17641 gccccgatgg cggcagtcct gcaaaaagag ttcggcgtag tcgaaggtct gatgaccacc
    17701 atccacgcct acaccggcga ccaaaacacc cttgacgcgc cgcaccgcaa aggcgatttg
    17761 cgccgcgccc gagccgccgc gctcaacatc gtgcccaaca gcaccggtgc cgccaaggcc
    17821 atcggtctgg ttatccccga attgaacggc aaactcgacg gctccgccca acgcgtccct
    17881 gttgccaccg gctcgctgac cgaattggtt tccgtcctcg aacgccctgt aaccaaagaa
    17941 gaaatcaacg ccgcgatgaa agccgccgcc agcgaatctt acggctacaa cgaagatcaa
    18001 atcgtttctt ccgacgttgt cggcatcgaa tacggctcgc tctttgacgc aacccaaacc
    18061 cgcgtgatga ccgtgggcgg caaacaactg gtgaaaaccg ttgcctggta cgacaacgaa
    18121 atgtcctaca cctgccaact cgtccgcact ttggaatact tcgcaggcaa aatctaaggc
    18181 ttgaacaaac ctgtggataa gtaaatgccg tctgaacaca ttgcgttcag acggcattgt
    18241 ttatctgtat agtggattaa atttaaacca gtacggcgtt acctcgcctt gccgtactat
    18301 ttgtactgtc tgcggcttcg ttgccttgtc ctgatttaaa tttaatccgc tataacttga
    18361 tattgcggct tatggcgaac aaacaacagc cgtttgttca actgctatgc ggcaaaaccg
    18421 gatttttgcc cgatttaaag cggggttggg gaaagatcaa taacggttgc acggttttcc
    18481 tcgatttgaa atctgatttc gtaatccgca atattcatca cataaatccg ttcgggaata
    18541 ttctgatagg cggggcgcgg atcttgggca atgctttggc tgataaggtt tttggtgttt
    18601 gcagataaat tttccgcgcc gatgttttcc tgccaaacga cttccaactc tacgggtttg
    18661 ccgctgacga aaccagatgc ggcatcgggt ttggattcga caaaggggat ataaggcttg
    18721 atgtccacaa tcggcgtgcc gtcgagcagg tctgcgccgc tgcaataaag gcggacgggt
    18781 ttgccggttt cgatgcggtt gagttttaag agtgatagtc cgagatggtt ggggcggtgg
    18841 gggctgcgcg tggcgaatac gcccattttt tgtttgccgc cgagccgtgg cgggcgcacc
    18901 atttgtgccc agccttcatc caatacgccg tgaaaaataa aacttatcca cacataatcg
    18961 aaatcttcca gcccgcgcac gctgtctgcg gtaaattcgg gattcagctc gatgcatact
    19021 tctgcggcgg agactaaacc gggctggcgg gcgatgccga atttctgttt gtagggcgat
    19081 cgggcggtgg cgatagggac gatggtgtaa gtcatgagga ttgtggataa gtttttcgga
    19141 aaaaccttat cttatcacag cctgttttac cgcttttggg cagtaatttg ttataataca
    19201 gggcatttta attgcgttga aatgatgaga tgattgtttc cattgatgtt gatgcacaaa
    19261 aaacttttac gcctttgtgt cctaaagagc tgcctgtaaa cgaggggcat ttgattgtcg
    19321 aagaattgaa tgcccaagcc gctttggcgg atttgcgcgt gatgacgaaa gatgcgcatc
    19381 atatggtagc gaaatggctt gtggataacc ctgttgatat gttgaagccg acaggtttgc
    19441 ctgatgcgga tttgacttgg gtggctcatg cgatggtcgg tacgcgcggc tatgaattat
    19501 tggacggact gccttctgcc aaagaatacg attattgcgt ttggaaaggt gttgatcctg
    19561 aattgcatcc ttacggcgcg tgttttcacg atattgagga aaaactgagc actggattga
    19621 ttgagtggct gcgttgtcaa aatacggata tggtcattgt cggcggtttg gctacggatt
    19681 attgtgttaa aacaacggtt ttgcagttgc tcaaaggtag caattggcag gttatcgtca
    19741 atgaagcggc ttgtcggggt attgcgccgg ataccattga aacggcatgg caggaaatgc
    19801 gttctgccgg tgctgtcatc ttaaaaaacg ccgaagaaat taaaaaatat attaataatc
    19861 aataagttaa aatcgtttca cgtgaaacat acttcttaaa tatgaaaatt ttgcttgtcc
    19921 gcttgtctag tatgggcgat ttaattcaca ctttgcccgc aatcgaagat ttggcgcgac
    19981 aatgtcctga tgtagaactg cattggctat gtgaggctgg atttgcagat attgcgcgcc
    20041 tgcatccgtt tgtaaaaaaa atccatgtga tgaaatggcg gcaatggcgc aaacatctct
    20101 ttcaggctga aacttggcgg gaaatgggtc atctgaaaca gactttgcgg caggaagcat
    20161 ttaattttgt attggacagt caaggtctga ttaaaagcgc gtgtttcgct aaaatggcga
    20221 aatcccctat ttatggtttg gataaaaata gcgcgcgcga gggactggct gcgctggcgt
    20281 atgcgaaaac atacgctgta ccgaaaggga aaaatgctgt ttggcgcaat cgtgagctgt
    20341 tcgcccaagt atttgggtat gcgatgcctg aaacgcaggt atttggcttg actgttcctg
    20401 aggcaggccg tctgaaaaat ttagcgcagc cgtattatgc ggctttgcat gcgaccagtc
    20461 gggacagtaa gttatggcct gtggaaaact ggcggtcgtt gctgcaaaag ttgaatgaag
    20521 aacagcaatg caatatttac ctgccttggg gaaatgaaga tgaaaaaacg cgtgccaaac
    20581 aaattgcaga cggactgccg tttgctattg tgtgcgataa aatgaattta ttgcaggcag
    20641 cgtatctgct gaaacacgcg gtcggaattg ttggcgtgga taccggtttg ctgcatttgg
    20701 caaacgcctt ggaaaaacct gtggtcggca tttataccga taccgatccg attaaaacag
    20761 gcgttcaggt ttcgccaatt gcaaaaaatt tgggcaatat cgggcagatt ccgaccgcag
    20821 atttggttta tcaagcgctg atggattgtg ttgcagcaga taaaggctaa agggtcgtct
    20881 aaaactgata tggcggcatg atattttcca atttattaga tacaggaatg tatatccgct
    20941 agtctgagta aatgctttaa aaatggtata atcggcaagc attatccgaa agatttttga
    21001 atgaattcgt ttttaaggaa aacggatttg gttttatagg gaagaaggtt tggtttcgct
    21061 ggatggtgaa agcagcgttc tttttaggag ggttcaatga aagcactggt cgcagtaaag
    21121 cgcgtagtgg actacaacgt caaagttcgt gtaaaagccg atggttcgga tgtggatatc
    21181 ggcaatgtca aaatgtcgat gaatccgttt gacgaaatcg ctgtggaaga agctgtccgt
    21241 ttgaaagaag ccggaaaagt aagcgaaatc gtagcggttt ctttgggtga gaaaaaatgc
    21301 gaagaaacct tgcgtacagc tttggcgatg ggtgccgacc gtgccattca tgttgaaacc
    21361 gatactaaac tggagtctct ggccgttgct aagttgctga aagccgttgc ggacaaagaa
    21421 aatccgcaaa ttttcttttt gggcaaacaa gcgattgatg atgatgccaa tcaagtggcg
    21481 caaatgctgg cagctttgct gaatgcggcg caaggtacgt ttgcttcaaa agtacacatt
    21541 gaaggtggtg aagtacagat tgtgcgcgaa attgatggcg gcgaagaaac catagcatta
    21601 aaactgcctg ctgttatcag tgctgatttg cgtttgaacg agccgcgctt tgtcaaactc
    21661 cccaatatta tggcggcaaa gaaaaaacct ctggaaaaac tggcgcctgc cgatttggct
    21721 accgatattt tacctcgtct gaaaacggtg aaatttgccg aacctaaggc gcgtcaggca
    21781 ggcgtaaaag tagcaagcgt tgccgaattg gttgaaaaat tgaaaaacga agccaaagtg
    21841 atttgaggag gctgaaatga gcgtattgat tattgtcgaa cacgacaaca aacagttgaa
    21901 tcctaccact ttgcatgctg ttaccgctgc cgccaagctg ggtaaagtcg atttattggt
    21961 tgccggaagc ggtgcatctg ccgtagtaga gtccgcaaaa caagtggcgg gtgtggaaaa
    22021 agttttggtt gcagatgctg cccattatgc cgaaggtttg gctgaagagt tggctccgct
    22081 ggttgttaaa ttggcagcgg attaccgcta tgttgcagca acggcaacta cattcggtaa
    22141 aaaccttttg cctcgcgtag cagccttatt agatgtaccg caaatttctg atttgaccga
    22201 gattgtggat aacacgattt ttgtgcgccc catttatgca gggaacgcat ttgaaaccgt
    22261 gcaagttgat tcggaaaaat tggtgctgac cttccgtgcg acggcttttg atgcagctcc
    22321 tgtacaaggt ggtcatgccg aggtaattaa tgttgaagca atccctgctc aaaacctgag
    22381 tcgttttgtg aaccgcaagc tttctcattc cgatcgtcct gaactgactc aggcaaaagt
    22441 gattgtttcc ggtggtcgtg cgttgggtag tgcggaaaaa ttcaatgaag tgctgacacc
    22501 gttggcggat gttttaggtg cggcaatcgg tgcatcccgt gcggcggttg atgccgaata
    22561 tgcgccaaac gatgctcaag tcggacaaac cggtaaagta gttgcgccgc aactctattt
    22621 tgcaatcggt atttcaggtg cgattcaaca cgttgccggt atgcaagaca gtaaggtgat
    22681 tgttgcaatc aataaagatg ctgatgcgcc gattttcaat gtagccgatt acggattggt
    22741 tggcgattta tttgaaatcg ttccacaatt aacagaagta ttgaaaaatt aatgtttcac
    22801 gtgaaacgtt cagacgacct tgttattaat gaggtcgtct gaaaacattt cagcatgatt
    22861 acgacttata aaaccattac ctcctcgacg caggctgagt ttaaagataa aggcagccgt
    22921 tttattgtat ttgcctatcc gattcggaca ttggctgatg tgaaaaaata cctcgatccg
    22981 ttaaaggaag agcatcataa agcacgacac tggtgctatg cctatcgttt gggtgtggat
    23041 ggtgtgcaat ttcgtgccaa cgatgatgga gagccatcag gaagtgctgg acgaccaatt
    23101 ttgggacaga ttgattcggt gggtattacc gacgttttgg tcgtggtcgt ccgctatttc
    23161 ggtggtactt tgttaggtgt tccaggtctg atacatgcgt acaaagaggc gacggctcaa
    23221 gcgttggcag ttgcagaagt agttgaaaag aatattgaaa aaactgtttg gctgaaatgt
    23281 gaatatccgt ttttgaatga agcgatacgc attgctaaac aatatcaggc ggatgtattg
    23341 gagcgggatt tgcagttgga ttgccgattg acggtcagtt tgtcgttggt aaattatgag
    23401 gcctgtgttg cggcttggaa gagtacacgg cagattgaag taaatacgga aaagcctttt
    23461 gaatgaaagg tcgtctgaaa tgctttgtga tggtatttca gacgacctct tcgctgacta
    23521 atcttcttgt gtatatttgt ctaaggcatc gacgctggag cgcaaatata accaagccag
    23581 ttgatggaat tcgcccagcg cgttccatag tgcatcttca ctcataatgc tgtattcgcc
    23641 ttctgtgcgc aaatccacct ccgctccatc ttcaatagcc cgatgacaag cctgatattc
    23701 gtgggtataa aaattgtcca tatcgcgaat caaagcgacg gcatgtttcc aacggcggat
    23761 gtcttcttcc aattgcggta gtaaatgaca ccattcgcgg tatttgcttt ggatttcatc
    23821 aatacgtttt tgcatggttg cttgtccttt tttaatttat gaataagttg aataggttgc
    23881 agagtggaat gaagcggtct tttgtttgat tctactaaaa tttcttcaga tgggataaaa
    23941 gtccctttgt atcgagtttt cgttaaaagt acatatttgt caaggagatg gcatgaaact
    24001 gctggttatc ggtaatggcg gtcgcgaaca cgcgctggct tggaaattgg cgcagtcgcc
    24061 taaagtggaa acagtatttg ttgcgtctgg taatgccggt acagcgattg aacccaagct
    24121 gcaaaacatc gacttgactg cgcatcagga tttgattgaa ttctgtcgta aagaaaatat
    24181 tgtttttacc gtcgtcggcc ctgaagcgcc tttggcggca ggtgttgtgg atgatttccg
    24241 tgctgcagga ctaaaaatat tcggtccgac acaatatgcg gcgcagttgg aaagttccaa
    24301 agatttcgcc aaagcattta tggcgaaata caatattccg accgcgcaat atcaaacctt
    24361 tgaaaacgcc gatgccgcac atgattacgt caatcagaaa ggtgcgccta tcgttatcaa
    24421 agccgatggt ttggcggcag gtaaaggcgt gattgtggcg atgactttgg atgaagcgca
    24481 tgctgcgatt gatgatatgc tgctggacaa caaaatgggc aatgccggcg cgcgtattgt
    24541 gattgaagat ttcctgcaag gcgaagaagc gagttttatc gtcatggttg atggcaataa
    24601 tgtactgcct atggcaacca gccaagacca caagcgtctt ttagacggcg acaaaggtct
    24661 gaatacgggc ggtatgggtg cgtacagccc tgcgcctgtg gtaacgcctg ttgtgtacga
    24721 acgcgcgatg aatgaaatta ttttgccgac cgtagcaggg atgaaagcgg aggggcatga
    24781 gttcaccggt ttcctgtacg caggtttgat gattgatcaa agcggtgcac cccatacgat
    24841 tgagtttaac tgccgtttcg gcgatcctga aacccagccg attatgagcc gactgaacag
    24901 cgacttatcg gatttggttg aagcggcaat agacggcaaa ctcgatagcg taaccgcgga
    24961 atggagcccg caaactgccg tgggcgtggt actggcggcg caaaattacc ccgaaacgcc
    25021 caaaaaaggc gatgttattt ccggtctgga tgctgccaac caagtaggca aagttttcca
    25081 tgcaggtaca acggcaaacg agaaaggtga cgtattgacc aacggcggac gcgtattgtg
    25141 tgttgtggga ttgggcgata atgtggcgca agcgaaagct aaggcatatg gcgcgttgga
    25201 aaaaatcagt ttcgacggga tgcaataccg taaagacatt gccgataaag cgattaaccg
    25261 ttaattagaa agaggatgat gccgtctgaa gccaagtatg actgatgtgc ttcagacggt
    25321 attttatatt atgttatttc cgagaattat cgcagcatca gcgcaacgta agttaagcgt
    25381 ttatttgaaa aaaggcggtt tggtcgctta tccgaccgaa tcttgttatg gcttggggtg
    25441 tcttccgaca ttagccaaag cactcggtaa gttggcgcat ttgaaaaaaa gaccgcaaca
    25501 caagggtatg attgtcatcg ggaatcaatt tgagcaattg cagcctttac ttcaaatgcc
    25561 gtctgaaaat ctgcaggata tgcttagaaa agaatggccc gcccctaaaa cgttcctatt
    25621 atccgccaaa tcctgtgtct tacctgagtt gcgcgggaaa cagcgcagca agttggcggt
    25681 cagggttccc gcccatgtag gtgcgcgccg cttgtgtcag gctttgcaaa cgcctttggt
    25741 ctcgacttcg tgcaaccgcg caggcaaacg cgcgtgtcgg acggagaggg aagtcagaag
    25801 gcaattcgga cgcgatgtgt ggattgttgg tgggagaatc ggcaggcaga aatcgccgag
    25861 tcagattatt gacggggaaa caggtaaacg tttgcgttag atatgggtac ttgaatgaaa
    25921 actttttttg catacacaaa gaaaaaattt ttcggatagc atttctctac atgattgtta
    25981 ttgcacagag ataaaggtag aaggacgcaa cgtctgcttt aattttgagg acggttttgt
    26041 cgttccggac aataatccga acaatcaagc gggcaagcat ctgaaaacag atttttcccg
    26101 tgttgtttta atccataaaa atgaaaacgc cgaagatgat ta
//
LOCUS       DAWKOD010000011        24210 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000011 DAWKOD010000000
VERSION     DAWKOD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.011"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   375..1400
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=441..476
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(1464..1790)
                     /gene="cas2"
                     /locus_tag="WJK73_000343"
     CDS             complement(1464..1790)
                     /gene="cas2"
                     /locus_tag="WJK73_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ7556881.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(1783..2697)
                     /gene="cas1"
                     /locus_tag="WJK73_000344"
     CDS             complement(1783..2697)
                     /gene="cas1"
                     /locus_tag="WJK73_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ7556882.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(2764..6012)
                     /gene="cas9"
                     /locus_tag="WJK73_000345"
     CDS             complement(2764..6012)
                     /gene="cas9"
                     /locus_tag="WJK73_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ7556883.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            complement(6290..9724)
                     /gene="dnaE"
                     /locus_tag="WJK73_000346"
     CDS             complement(6290..9724)
                     /gene="dnaE"
                     /locus_tag="WJK73_000346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ7556884.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKTCRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10182..10496
                     /locus_tag="WJK73_000347"
     CDS             10182..10496
                     /locus_tag="WJK73_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7556885.1"
                     /translation="MCCRFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EV"
     gene            10474..10683
                     /locus_tag="WJK73_000348"
     CDS             10474..10683
                     /locus_tag="WJK73_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556886.1"
                     /translation="MDTTLKYKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(10725..10967)
                     /locus_tag="WJK73_000349"
     CDS             complement(10725..10967)
                     /locus_tag="WJK73_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556887.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            10976..11812
                     /locus_tag="WJK73_000350"
     CDS             10976..11812
                     /locus_tag="WJK73_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ7556888.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(11864..13156)
                     /locus_tag="WJK73_000351"
     CDS             complement(11864..13156)
                     /locus_tag="WJK73_000351"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ7556889.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13212..15122)
                     /gene="pglD"
                     /locus_tag="WJK73_000352"
     CDS             complement(13212..15122)
                     /gene="pglD"
                     /locus_tag="WJK73_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ7556890.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15170..16345)
                     /locus_tag="WJK73_000353"
     CDS             complement(15170..16345)
                     /locus_tag="WJK73_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ7556891.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            complement(16423..17664)
                     /locus_tag="WJK73_000354"
     CDS             complement(16423..17664)
                     /locus_tag="WJK73_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ7556892.1"
                     /translation="MNKFFKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKGGKPFKMIKFRSMRDAVDKYGNPLPDSERLTDFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEVKPGITGWAQVNGRNATSWDERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVAELA
                     AALGTYGEIVFLDDRVQGSVNGFPVIGTTLLLENSLSPEQFDIAVAVGNNHIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESWIGTGACSRQQIRIGSRATIGAGTVVVRDVSDG
                     MTVAGNPTKPLAGKNTETLRS"
     gene            complement(17657..18824)
                     /gene="pglH"
                     /locus_tag="WJK73_000355"
                     /pseudo
     CDS             complement(17657..18824)
                     /gene="pglH"
                     /locus_tag="WJK73_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            complement(18844..19917)
                     /locus_tag="WJK73_000356"
     CDS             complement(18844..19917)
                     /locus_tag="WJK73_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7556893.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPPEFHFRKTEKECAVLGTLSRLDTVRAVHLMLDIFKKMVDRGMPVRLNVAGIG
                     EEMDNLKAQAKRLGIEDKVTFLGGIRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            complement(19914..21335)
                     /locus_tag="WJK73_000357"
     CDS             complement(19914..21335)
                     /locus_tag="WJK73_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ7556894.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGIWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(21530..22639)
                     /gene="ribD"
                     /locus_tag="WJK73_000358"
     CDS             complement(21530..22639)
                     /gene="ribD"
                     /locus_tag="WJK73_000358"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ7556895.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLESAGIKTECGLLENKARELNRGFLSSIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(22671..23135)
                     /gene="nrdR"
                     /locus_tag="WJK73_000359"
     CDS             complement(22671..23135)
                     /gene="nrdR"
                     /locus_tag="WJK73_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ7556896.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(23165..24007)
                     /locus_tag="WJK73_000360"
     CDS             complement(23165..24007)
                     /locus_tag="WJK73_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ7556897.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
BASE COUNT         4978 a         6014 c         7148 g         6070 t
ORIGIN      
        1 tttgcaagat tttagtttaa tcatcaaaca gggaacagca aaaatccccg ctgcaacggg
       61 gatttttgtt cggctctcgc ctgtgtgatt aacttcacta ggagaagcta aaatgcttaa
      121 attccttatg cgtgggaaaa agttgctgct gtccgttgaa gtgaaccaca agttcatttt
      181 agcggttgct ttgctgttaa gtcaatgcag tagctaaccc gaccacaatc aaaaaaacgc
      241 aagttttccg cctactctgt gagtgggcgg ttttttctta tttcaaatct gtgacacaat
      301 atctgtcgcc tttcttgcca taattgcact gtgtctcaat gactttaacg cacgttcgct
      361 tatcgcaacg gctggttgta gcgcccattc tcatttcgca gtgctacaat tttttgtatc
      421 cattcccagc cggaaattaa gttgtagctc cctttctcat ttcgcagtgc tacaatcttt
      481 aactttgacc gtgtgcaatc cagttagttg tagctccctt tctcatttcg cagtgctaca
      541 atctgaccac acctatccgc cagtgcttat gcgttgtagc tccctttctc atttcgcagt
      601 gctacaataa gccggtttat ggcaattctg ccgcgtccgt tgtagctccc tttctcattt
      661 cgcagtgcta caatatgcct ttttttgtac tgttgtttga acgagttgta gctccctttc
      721 tcatttcgca gtgctacaat aaattcgttt cagatagcaa acgcagtagt gttgtagctc
      781 cctttctcat ttcgcagtgc tacaatgccg gatatgacgg tgggcaactg gtacaggttg
      841 tagctccctt tctcatttcg cagtgctaca atatttcgct tttgtattga ttcaaggtgc
      901 ttgttgtagc tccctttctc atttcgcagt gctacaataa attcgtttca gatagcaaac
      961 gcggtggtgt tgtagctccc tttctcattt cgcagtgcta caatagtatt cgtcgatgat
     1021 ggaaactcga gcatgttgta gctccctttc tcatttcgca gtgctacaat ttgcgcatga
     1081 tggcaacgat aattaccatc gttgtagctc cctttctcat ttcgcagtgc tacaattacc
     1141 cacggcggaa accattgcca caaaacgttg tagctccctt tctcatttcg cagtgctaca
     1201 attcataccc aaatcatcgg catcctgttg gtgttgtagc tccctttctc atttcgcagt
     1261 gctacaataa tgacggcaga ctacggaaac gaattctggt tgtagctccc tttctcattt
     1321 cgcagtgcta caattcaaac gatgcgctgc cgtttttgcg gtcggttgta gctccctttc
     1381 tcatttcgca gtgctacaat acttcgacgg gaaatcctta tttcataagg gtttcccgtt
     1441 ttatttgtta tgaaaaaatg ggcttaaaat aacaataatt ggtctgaatt gacctttttt
     1501 tcttgggttt tcagctcgcc caacagcagt ttcatggcgg catattgctt ttctgttatc
     1561 tccaaacagc ggattgagcc ttcttgcgga aggtttgcac acagtctgtt gtggtgtttt
     1621 tgcaacgaat cgcggccttt gacgatacgg ctgtatacgg aaagttgcag catttggtat
     1681 ccgtctttta ataaaaactg gcggaattga ttggcggctt tgcgctttgc cgccgtgata
     1741 accggtaggt cgaagaagac gataatcctc ataaatttgg cctcactcat attggtattc
     1801 tttcaatggt aggatttcag gcagtttcag ttgtttggcg tttttatcgg ttacgccggc
     1861 ttgaaatgac gaaatcattt tgtcgatggc agccaaggtg ctgaaatgtt gccgttcgat
     1921 gctggtttga tagtagagga ttttgatgag gtgttgtttg atgccgagcg tcagttcagt
     1981 tttcagacgg ccttgttcgt acaaatgtat cacggtcagg tcggccaatg ggcgtaaggg
     2041 ttcgataaag tcatcggcga gattaaatgg attgagttca cttctatgaa acaagcctaa
     2101 tgcaggtaac caaccgtata aggtcagggc gcgtgcaact gcggcacgga gtacggcgta
     2161 ggtgtagttc aatgcggcat tgacggcgtt gttgtcatta cgggtaaatt tttcgccaaa
     2221 gagggcttga aagtagaggg cggcagcttg ggcttcacgg tttcctgtat cgcctgaacg
     2281 gacttcagat gccaaagtgc gcagccgttt ggctgcaaga tcattgcctg tttcgtctgc
     2341 gacaaatgct tggttcagga ttttttggcg gacgatatgt tgccagagtt gcttttttaa
     2401 gggttcgctt atgttcagtt gcagtttgag gattttaagt tggcggtgat attgggcata
     2461 aggcagccat tgtccgcagg gcaggaactg ctcgtcgcag gtcagcaggg ttgcaccatg
     2521 ctcggctaag gctgatagga gcggggcggt aatcagggtt tcgcgatttt cgatgatgat
     2581 aacggcgatg tcctctaatg gtacggtgtg ggactcgccg ttttgttgaa tcagcagttg
     2641 ccggcgttgc aaggatagtt tgccgccgtt ttgaataagc aggctgcgcc aagtcatgtt
     2701 tttcccctta tttttatata aaaaatccct gcttcaggcc gcctgaaaca gggatagatt
     2761 aatttaacgg acaggcgggc gttttttcag acggcatggt ctgatttctt tgcccagttc
     2821 gtcaatttgg tatttttgga atgaaagggc ggttttgacg ccgatacctt ccagtattcc
     2881 atttttgcca attttatgat caagatcatg aatgcgtata ttgatattac cggtgcctcg
     2941 atggcagctg gcaaagtaac caaacattct agcttttttt gttataacct cgactaaatc
     3001 attagggtgt aatgagaatt taaagttgaa actatcatca ataagttgcc aatcttcttc
     3061 atcttttcct tgtacaacag ccctatccgg caaaatccct ttcgctacct gccaactgta
     3121 aatcggtacc agataatact tgtcgccttt ctcaaacaca tctacgcgca ccatggttgc
     3181 gttgtcggca ataccgttat ggttgcgcac ccatacgccg gttttctgta cttgctctac
     3241 gcgtacggct tttacctgtt gggtgcggtt gcctgcttta tcgtatttgt aaaacggctc
     3301 ggcaaaggct ttggcaggat cgtctttatg tgcttccagc cgtgctttca gtgcttcgta
     3361 tagcttaggt tcgcgctccc gattgaccat tttttccaag tctttcagtt ttaactgtgt
     3421 taacggtacg cgcaacacgc tgacgccttc gtccagtcgt ttggcggatt tgacggtctc
     3481 catatgccct tgcccgctca tcttccgatt gggcgcgcgt gaaacaaaca gtggcgtaac
     3541 gtattcgtgt acggcttcgg ggcgagatga taatttttcg gcaagcaacg tgcgcagttt
     3601 ttctggggta tcggcttctt cgaattcggg tttgccgtcc ggtttgccga agacgcgaat
     3661 catgacttct tgtgcgaaaa attcccaagg ttgtgggaag tgtgtttttt gatgcagcac
     3721 ttctcctgtt tctttgtcta tggttttacc gtcaaacgcg ttcatctctt tatagcgtac
     3781 aaaacgggta attttctgct gcatggcaac ggtcgagcag gcaacgacga cggcgtccaa
     3841 ggcgtgatgg cggtcgtttt ccgcacgcac tttgcgcaat ccccaaaagc cgcgcaacag
     3901 attggtaatt tgtccgttgg atgcaaagac acgtttcttg cctttacctg tcagccgcat
     3961 acggtcggca acaaattgac acaggaaacg gttgacgtag cgcgtgtcgt tcagattgcg
     4021 ttctttaaag ccgtcttcat cgaatttttg cagcagaatc cgttgttttt tactgcgcgg
     4081 gaaacggctg gtttcgacac gcgctttaaa ttcctgccat tcgcggctgt tgtctttgcc
     4141 gttgaagtat tcgtaagggg tttgattgcc tttgttttgg ttttcgctgc ccaataccag
     4201 tactttattg ttgaaactgt cgtcccatgt gcgcgagaac ggcagggcat ggtcgatttc
     4261 gacatagcct ttttcgttca gacggcctaa gttgatttct ttgcccgaat acaggcattt
     4321 gccgtgttgt tgctcgtaca gacgcagttt cagaatatct ttggatttgg gttctccgac
     4381 aaaattgggg aaatactctc ggaatttggc ggcggctttt tccctgtctt tgcggttttc
     4441 ttcttggcgt ttctcaattt ctttgcggtc tttaaacgat ttacctactt cccttgcagt
     4501 ttcaatatgg atacgagctg gggagccgta acggcgtacc acgccgttaa tgaccttacg
     4561 tgcttgagat aaggcgcgca agacgacggg gttgcggatt tcgtcggcgg gaatcggcgg
     4621 cagataaatc ttttcttccg tattcttctt gccgtaatgg tctccgtaga tttcggcgca
     4681 ggcttcatcg taacgtttgc cttgttccat tagaggcaca attcggcgca atgctttcaa
     4741 ggaaatttgg acgaacttat cgaagctgat gtgtttcaac agcgcttcta agatttcggg
     4801 ctgtatacgg tctttcagac ggcctgtaat gtcttcatcg gttttgaaca gggagaatgc
     4861 cgtgccgatt tcgtcttgta attcgggaga aaggtttaat ggggattttt tgtctttcaa
     4921 tccttctttt tccagtgcac ggctgatggc atggtaggcc ttcatttcca tcaatgttga
     4981 ggcttcggca ttgtctttac catagcgcaa gcctttgaaa aaggcggtat cttctaaacc
     5041 cagcagctta cgggcttgtg cgtaagtcag tttggatttt ctgtatggct cgtccataag
     5101 cgtggcgcgt tcggtatcgg tcaatggccg ctcgctgcct tgctctaaaa tacgcaggtt
     5161 gttcagcttg gtcagccaga tgaaacgttc ggctgtgtag gtgtttttag cggctttcgg
     5221 ctctgccggt tcgaaggtgc aatgccccaa cattttttga acggcatcgc cggacagggc
     5281 agggcgttgc gtcatcagta gggtttcaat accttcttta aggccgcctg aaacatgcgg
     5341 attgccaaat tctttttgtt tttcaaacag caaaatcagc tccgcctgta aatctttgcg
     5401 gctgaacgta tgcgaataat cgccgcgctg attgcggata tggccgcttt ctttctcaaa
     5461 tttatttaaa gccaattcgg ccggtgtgcg gaaatcgcct gtctgtaagg catgggcatt
     5521 gtcggctacg cctttgagca aagcgccaag ctctttatcg gcagtttcgc cctcgttttt
     5581 tctttgcgac aaataaccgc ggtgtttgat taaatgcaac aagactgccg accactctaa
     5641 aggcgtcagt ttgcggtcta atgcggctgc gcgaagttgc catggtgtat tcggtaagga
     5701 tttgatcaag ccgttttcgt caaaatcagc ggcttgtaat acgccttcgc gtttcaatag
     5761 gcggcgggcc cgaagcaggc gatgggcgcg acggcgggtc aggcggcgga cactgcgcgc
     5821 caaacgcctt gccatggcaa gggagtcgcc tgttttcggt acttcggcac gctcaaatac
     5881 gcgcacgccc aaatcaatca ggcagattgg gttttcatct tcgtcaattt ctaccatcgc
     5941 ccagccgacg gatgcgatgc cgatatcgag gccgaggatg tagttgattg gattaggttt
     6001 gaaggcagcc atttattact ccgtaaacaa cgttgcaaat aatgataata tatcttattg
     6061 tcttatttat atacaaatag attattgact tatcatctca caacggctag aatcccaaac
     6121 atattgtcgc actgcgaaat gagaaccgtt gctacaataa ggccgtctga aaagatgtgc
     6181 cgcaacgctc tgccccttaa agcttctgct ttaaggggca tcgtttattt cggttaaaaa
     6241 atgccgtctg aaaccgattt tgggcttcag acggcattta tattttgggt taccagttga
     6301 cgcgcaccga ccggctgccg agcaatgttt ccagttcgcc gaacaatgcg gagctcggtg
     6361 taaccgtcca tttcggcggc acttgaagcc tgcccgacgc tttttcgttg gcatacgaca
     6421 gttgcagcgg gatgcgcggc gtgtcgggca gttggtgggc ggcaagcagt tgtaccagtt
     6481 cgccgatgtc gtgatgcggg gcgagggcga ggctgaggct gcgggcgtag cgttcgcgcg
     6541 ccgtttgcag ggtcatgact tggtttgcca gaatacgcag cccgtcgccg ccgccgtagt
     6601 cgtcgcggct gactttggat tcgataatca gcacttggtc ggctttgagg cagtcggcgc
     6661 agttttccaa cgtctgaccg ccgaccatga tttcaacctg cccgctcaaa tcttcaaggc
     6721 tgacgaaggc gattttgccg cgcttgccca tcatcgtacg cacggcggta acgaatccgg
     6781 cgaggcgcac gctgtcttgc ggtttgagtt tcgacagttt ctgcggggcg atttggcgga
     6841 cttcttgggc atacgggccg aacgggtggc cggacaggta aaagccgatg acggtttttt
     6901 cttcggcgag tttttccgat tcgctccaca tcggcgcgtc aatgagccgc accggttcga
     6961 tggcgtcttc catcatgtcg aaaagcccgc cctgattggc gttggcggct ttttggtcgg
     7021 cgttgttcat ggcgaggtcg atgtttgcca agagcatggc gcggttgggt tcgatgctgt
     7081 cgaacgcgcc gccgcgtatc agggcttcga gggtgcggcg gttcatatgt tctttgccga
     7141 cgcgctcgca gaagtccaag aggccggtaa acttgccgcc gctttgccgc gcggcgatga
     7201 tggattcgac ggcggcttcg cccgtgcctt taatcgcgcc gagtgcgtag cggattttca
     7261 tgttcggata cggcgtgaag cggtagtcgg attcgttgat gtcgggcggc aggaactcga
     7321 tgccgttggc gcggcagtcg tcgtagaaat gcttgagctg gtcggtgttg tccaattcgg
     7381 acgacatcgt cgccgccata aattcggcgg ggtagtgcgc cttgagccat gcggtctggt
     7441 aggaaatcag ggcgtaggcg gcggcgtggg atttgttgaa accgtagccg gcgaattttt
     7501 ccatgtagtt gaagatttcg tcggattttt cgcgcgaaat gccttgtttc gccgcgcctt
     7561 cggcgaagat ttcgcggtgt ttcaccattt cttcgggctt tttcttaccc atcgcgcggc
     7621 gcagcaagtc cgcgccgccg agcgagtagc cgccgataat ctgcgccgcc tgcatcactt
     7681 gttcctgata caccataatg ccgtaggtcg gcgcgaggat gccttcgagc agcggatgga
     7741 tgtattggaa ttcctggcct ttcatgcgcg cgacgaagtc ggggatgttg tccatcgggc
     7801 cggggcggta gagcgatacg aaggcaatca gttcttcaaa cttggtcgta tgcgccgttt
     7861 tcagcatttt tttcatgccg gtcgactcga actggaacac ggcggtggtg ttcgcatctc
     7921 ggaagatttg gtaggcggct tggtcgtcga gcggaatttt gccgacatcg acgatatcgc
     7981 cggtggtgtt tttgatgttg ttctgcgcca tttcgataat ggtcaggtta cgcagaccta
     8041 aaaagtcgaa tttcaccaaa cccacgtctt ccacgtcgcc cttgtcgtac atggatacgg
     8101 gagaggcgga ttcatccgcc tgatacacgg ggctgtaatc ggaaatcttg cccggcgcaa
     8161 tcaacacgcc gcccgcgtgc atacccagac cgcgcgtcaa atcttccagc tttttcgcca
     8221 gcgtaatcag ttcgtccgct tcttccgctt cgattaattc ctgaatctgc ggctccgcct
     8281 tcatcgcgtc gtccaaaccc agaggtttgt tggcttccaa cggaatcagc ttggacagtt
     8341 tgtcgcacag cataaacggc agctctaaca cgcgccccac gtcgcggatg accgctttag
     8401 atgacatcgt gccgaaggta acaatctggc tgaccgcctc cgcgccgtat ttttcgcgca
     8461 catactcaat cacgcggccg cggtttgcct gacagaagtc aacgtcgaag tcgggcatgg
     8521 agacgcgttc ggggtttaag aaacgttcaa acagcagcgc gtatttgagc ggatcaaggt
     8581 cggtaatctt caatgaatac gccaccagcg agcctgcgcc cgaaccgcgc cccggcccca
     8641 ccggacagcc gtgcgttttt gcccagttga taaagtcttg tacgataagg aaatagccgg
     8701 gaaatttcat ctggatgatg atgttcagct caaaatccaa acgttcctga tattccggca
     8761 ttttttctgc gcgttccgct tcgtcgggat aaagctgaac catgcgttcc tgcaaaccct
     8821 cgttggagag tttgatgaga tagtcatcga gtgataaacc gtcgggcgtg gggaaaaggg
     8881 gcaggaagtt tttgcccaat gtgatgtgca ggttgcagcg tttggcaatt tctaccgtgt
     8941 tttccaaggc ttcaggcaaa tcggcgaaac gttcggccat ggtttccggc ggaatgaaaa
     9001 actggcccgg cgtgaaatcg cgcggacgtt ttttgtccgt caatacccag ccgcctgcga
     9061 tacacacccg cgcctcgtgc gcgttgaaat cgtcgcggct cataaactgc gtcgggtggg
     9121 tcgccaccac cggcaaaccc agttcctccg ccagcttcac gctgccggaa acgcaagcct
     9181 cccattcggg gcgttcgggc aggcgttgca gctctaaata aaacgcatcg ggaaaccatg
     9241 ccgcatattt caacgccgcc gcacgcgccg cgtcttcatt gccgttcaag agattcacgc
     9301 ccacttcgcc gtaatgcgcg ccgcttaaac aaatcaagcc gctgttgtcg ccgttttcca
     9361 gccattcggg attgagttcc gcatgatgga tattgcggtc tttgccgaca taggcctctg
     9421 tcaaaagctc gctcaagcgc agatagcccg catcgttgcg gataatcagc atggcgcgga
     9481 acggcttgtc gggcgcattc ggattgccta tccacacgtc cgccgcgccg atgggtttaa
     9541 tccctgcgcc gcggcaggtt ttatagaatt tcaccaaacc gaattcgttc atcaaatcgc
     9601 tgatgcccaa agcaggcaaa ccgtattcct gcgctttggc aatcagtttt ttaatccgca
     9661 ccataccgtc ggtaatcgag aattcggtat gcaggcgcag ggggatgtag gtcggctcgg
     9721 tcatggcaaa atcggcgtga acaataaaag gcgtattgta gcagggttgt ctttagatgg
     9781 cggtgtaggt aatgctgttt cggattcaga cggcatgacc tgcaaatgtt tttgagcttt
     9841 tacgacggca aaaaaatgcc tcctaccgtg tggtggaggc ttcccaagga gtattgatag
     9901 atataaagga ctatcaaact agttataaag aactatatac cttattcgga cggatggcaa
     9961 gcagttaaat cgattttacg ttcaaacagg tttttgattt cgttttgatg ccgattgccg
    10021 gtgtatcggg cagttcgcgt ttgaggatgt gcatcagcgt caatgcggat tcgtcgggga
    10081 acaggatttg cagcttgccg ttggacagga tttctttgat cggatggcgg catatggggt
    10141 ttcagacggc atttttactt ttacggaaca gctggtgtgg gatgtgctgc cggttttgcg
    10201 gtaattttgg gtacggcggc ggcaggtgct acaataacgg cctctttctg caagggaaca
    10261 ttatggaagc caccgtctat ctcgaagaca acgaatacat cgccctgtgc gacctcttga
    10321 aattggccgg acttgccgaa agcggcggac aggcaaaagc atttatcgcc gaagggctgg
    10381 tgttgcgcaa cggcgaaacc gaaacccgta aaaccgccaa aatacgcggc ggcgaagtca
    10441 tcgagtttga cggcgcgcgc ttggaaatcg ccgatggata cgaccctgaa gtataaagcc
    10501 gaagcccttt tgggcgagcc gcttttagaa gaacccgtcc gccctgagtt gtgggaatgc
    10561 tgcggcagcg actgcggcga ggcgtgcatt cagacgattt actgggcgga taaggcacgc
    10621 tacgacgcgc aacggcggaa gttgaaagaa gcgggttggg cggaagacga aaatgccgtc
    10681 tgaaaccagt tcggcttgat aaaaccacgt tttcagacag tcttttatag tagattaaaa
    10741 ttgaaaacgg tcatatcgcc attccctcga aagcaggaat ccggaagttt gaaatagcgg
    10801 ttaaccttaa acatttccaa taaatcaaag tctggattcc cgcctgcgcg ggaatgacgg
    10861 catagcggct tttcctttgc atttgccata cacttgtttt ttgaaatcac cgccgccaca
    10921 ccgcccgaca atattgaaac accctgccca ccatttacca cgcacataac gccttatgat
    10981 agacctgcac tgccattcca ccgtttccga cggtatgctc tcccccgccg aagttgtgcg
    11041 cctcgcgcat caaaacggct gcacgctgct ggcgttgacc gaccacgacc acaccggcgg
    11101 catcgccgaa gcgcgtgccg aagccgacag gctcggtttg cgcctgatta acggcgtgga
    11161 aatctccgta acgtggcgcg ggcgtaccat acacgttgtc ggcttagact ttgacgaaca
    11221 agacgaaaac ctgcaaaacc tgttggcgca agtgcggcaa ggccgtctga aacgcctcga
    11281 agccatcgcc gccaagctgg aaaaacgcgg catttcgggc gcatacgacg gcgcgctcgc
    11341 gctggcggca aacaaagaaa tggtcagccg cacacacgtc gccgagttcc tcattcgggt
    11401 gggacacgtc aaaaacaagc agcaggcgtt caccaaatac ttgggcgacg gcaaatcctg
    11461 cgccgtccgc cacgaatggg cgacgctgga agactgcgtc tccgccgtca acggcgcggg
    11521 cgggttggcg gtcatcgccc atccgatgcg ttatgatttg tccgccaccg ccaagcgcaa
    11581 tctgtttgaa gaatttaaaa acctcggcgg cgcaggcatc gaagtccaca gcggcaactg
    11641 ctgcaaaaac gaccgcctca actacgcgct tttggcagaa cgcttcggta tgctcgccag
    11701 cgcgggcagc gacttccacc gtttaaacga ctttagcggc ggcattctcg gcgcgtgtcc
    11761 cgaactgccg gaaaactgca ggccggtgtg ggcgcatttt tcccgatatt gaatgcagat
    11821 aaaaatgccg tctgaaaacc tttcagacgg cattttttgc ggtttaaacg ttgtcgtaca
    11881 gttttcggac ggttttgccg atggcggcga tgcctttttc cagcgtttga gcgtcctgcg
    11941 cgatgctcat gcggatgcac tcgcccgcgt gcgggtaatc ccgcgtgtcg atgccgacga
    12001 agaaatgctc gcccggaata atcagcgtac cttcggcttt gagcatttcg tacagggttt
    12061 gcgaagaaac gggcaggttt tcaaaccaga gccacaggaa aatcgcgcct tcgggtttgt
    12121 ggattttcat cgggtacgcg cccagctcgc gcttgagcag cgagacggcg gtttgcgcct
    12181 gattgcggta aaacggccgg atgacttggt cggcaagccg tttcatctcg ccgctttcca
    12241 gcagcggggc ggcgatggcc gcgccgaagc gcgtgggggc aaggttcaca atcgcgttca
    12301 ggctgctgac ggctttgacg acttcgggcg cggcgacgat gatgccggtg cgaacgcccg
    12361 gcagaccgac tttggaaagg ctgaagcaga ggatgatgtt ttcatgccaa ttcagcgtta
    12421 cgtcgctgta aatgatgttg gggaacggca ttccgtaggc gttgtcgata atcagcggaa
    12481 tgccgtgttc ttgcgccaaa gcgtccagcc gcgccatttc gctgtcggtc agcacgttgc
    12541 cggtcgggtt ggtcgggcgc gaacagcaaa tcgcgccgat tttgcccgct ttgagttcgg
    12601 gcaggttttc cagtgcgtca aagtccacgc ggtatttgaa gaagcctgct tcgccttcgt
    12661 gttcgacgtt ttcgattttg ggcttgacgg aaacgaagtg ctgcccttcg acatgcacgt
    12721 cggcatagcc gatgtattcg ggcgcgagcg gcaacaaaat ggctttttct gcggatgtgc
    12781 cgtctgaaag gttgaatttg ccgccgaaga ggttgaataa atagaaaaac gcgttttgcg
    12841 aaccgttggt cagcgcgata ttgtcggcgg tcaggttcca gccgtattcg cggttgagga
    12901 aggcggtcag cgcgtcaatc agcgcggcat cgccttgggg attggagtag ttgccgatgt
    12961 tctcgacagc gtgttccgcc gccagtttgg agaatatgtc ggcgaacgcc cgatcgattt
    13021 ccggaatgcg cgccggattg ccgccgccga gcatgttgac gggcttgtcg cttttgagcg
    13081 cgtcgccgag gtcgtccatc agttggagga tgccgctgtg ttgcgtgaat ttttcgccga
    13141 atgctgagaa ctgcatgtca aactccgtgt gtgtaaggta ggttggtttg tacgttatgc
    13201 cgtctgaagg ttcagacggc attttttctg tgtgtttctc gtacccaaag caggtcgcag
    13261 atgccgtcgc tcggggcaaa gccggtcggg gcgttgatga gcagggtgcg gattgcctgc
    13321 tggtcgtaac ggtcgcaggc cgaacggatg cggtcgagca ggacagagag ctcgtgccac
    13381 ggcagcatcg cctcgtcagc cgtcataatg cgcggatggg cggttttgcg gacgttgtcg
    13441 ccgatgagca gctcttcgta gagtttttcg ccgggacgca gtccggtaat gaggatttcg
    13501 atgtcgccgt cgggttgttc gggtgtgttg ggtttgaggc cgcttagggt aatcatttgg
    13561 cgggcaaggt cgatgatttt gacggattca cccatgtcga ggacgaatac gtcgccgccc
    13621 gtacccatcg cgcctgcctg tatgacgagt tgggcggctt cgggtatggt catgaaataa
    13681 cgtgtgattt cggggtgggt cagggtaagc gggccgcctt ctgcaatctg tttttcaaac
    13741 agcgggacaa cggagccgga cgaacctaaa acattgccga aacgtaccat gctgaagcgg
    13801 gttttttgtc cgggttcggc ggcgagtgcc tgaaggcaga gttccgccat gcgtttgctg
    13861 gcacccatgg tgttggtggg gcggacggct ttgtcggtgg agatgaggac gaaagttctt
    13921 acgcccgatg tcgtggcggc aagcgcgcac tcgagtgtgc cgaagatgtt gttgcgtatg
    13981 ccttcgacgg tgttgaactc gaccatgggg acgtgtttgt aggcagcggc gtgatagacg
    14041 gtcgcaacgg aaaaggcggt catgacgtgt tcgagcagcg tgcggttttg caccgaaccg
    14101 agaaagggca ggatttcggt gtcgaggcgt ttttggatgc aggtttcgcg caattctttt
    14161 tcgatggcgt acagggcgaa ttcggataac tcgaacagca gcagcttttc ggggcggcgg
    14221 cggataatct ggcggcagag ttccgaaccg atggagccgc ccgcgccggt taccatgacg
    14281 gttttgcctt cgatgtcggc actcatcagg cggtcgtcgg gcgcgacgga atcacgcccg
    14341 agcaggtcgg acacagagat ttttttgagc gtgccgatgc tgatttttcc gtccatcagg
    14401 tctttcattc cgggaatggt caacacttcg cacggatagg cttccagttt gttgatgatt
    14461 cggcggcgtt gttcctgggt cgcgccgggg atggcgagca ggattttttc cacgccgtag
    14521 cgttcgatga ggaaggcgat ggcatcgggc tggtaaacgg caaggtcgta gatgacggtg
    14581 tgccacagtt tggggtcgtc gtctacaaag gcggcggcgg aatattcgcg catttgtttg
    14641 acggcctcaa gcagttgtct gcccgaccgt cccgcgccgt aaatgatgac agggatcatc
    14701 tgttttttgg ggtgttcgga caacagtccg cgcaaaacca tacgcgagcc ggtcacggaa
    14761 acaaacagca gtaagaaata gacaatcggc agggcgaggc gcagcctttc ttcaaaaatc
    14821 aatgtgttga ggaagaacaa tacggcggag gcgaggctgc ctgccagcgc ggtggagagg
    14881 atgcggaagc tgatgaagcg tgtaacggca cggtagagcc ccattctgac gaacaggccg
    14941 atggtcaacg gcgcggtcaa taggaaagac tgccagttgg cagggtcgaa ccattcgttc
    15001 gagtagtcgg ccttcaggct ttgggtaaac caaaaggcaa tgaaaatcat cagaaaatcg
    15061 tgtatgagga aacagatttt cttgatgttg cgcggtaggg caagcagggt ttccagagtc
    15121 atatcgtggg gcggtatgtg ctttcaggcg gcatatgccg tctgaagggt tatcgtgtgg
    15181 cttcggtcaa tacggcttcg atgtgttctt tgcagaacgc gatttcgccg tcggtcagcg
    15241 tcggatgtac taagaacatc aggctggtgt cgcccaactc gacagcattt ttcagccgct
    15301 ctttcggacg ccaaggcgtg ttgtcgaagg atttctccaa atacacttcg gagcagctac
    15361 cttgatagca ggggactttg cgcgcgttca gttcgttgac gatgcggtcg cgcgtccagc
    15421 cgtctttgag gtgttcgggt ttgacgaagg cgtagaactt atattgcgcg tgtccgatgt
    15481 agtcggcgac ttcaaccaag cggatacttt tgaatttgcc taggctttcc gccagcttgg
    15541 cggcgtgcgc ttgacggcag gcagtccatt cgggcaggcg tttgagctga atgcgcccga
    15601 ttgccgcctg catttccatc atacgccagt ttgtgccgaa gctttcgtgc agccagcgga
    15661 agccaggggc gtgttcgcgg tggtacacgg cgtcatagct tttgccgtgg tctttgtacg
    15721 accacatttt gctccacagg gttttgtcgt tggtggtaac catgccgcct tcgccgcccg
    15781 tggtcatgat tttgtcttgg cagaacgacc acgcgccgac gtgtccgata gagccgacgg
    15841 atttgccttt gtattttgcg ccgtgcgctt gggcgcagtc ttcaatcacc cacaaatcat
    15901 gttcttttgc caaagccata atgccgtcca tttcggcagg cataccggca aggtgaacca
    15961 cgataatcgc tttggtattc ggggtcagca cggccttgac ggtttccgcg ctgatgtttt
    16021 ggctgttcaa atccacatca gcaaacacgg gatttgcgcc cgcattcacg atgcaggagg
    16081 cggaggcgag gaaggtgcgc gaagtaacaa tcacatcgtc gcccgcgcct atgcccattg
    16141 ctttgagcgc ggcatcgagt gccagcgtgc cgttggaaag ggcgacggcg taccgcgtgc
    16201 cggcgaaagc agcaaattct ttttcaaatt cgcggcattc gctgcccgtc cagtagttga
    16261 ctttgttgga cagcaggact ttggaaacgg catcggcttc ttcttgggtg aagcagggcc
    16321 acggggaaag gaaagtgttc agcatggtgt ttggtccgtc ggttttcaga cggcatttcc
    16381 tcatttctgc cgtctgttgc ttaggctgtt tattttaatc gtttacgacc gcagggtctc
    16441 ggtatttttg cctgccaatg gttttgtcgg gttgcccgcg acggtcatgc cgtctgaaac
    16501 gtcgcgcacg acgactgtgc ccgctccaat ggttgcgcgg ctgccgatac ggatctgctg
    16561 gcggctgcac gcgcctgtgc ctatccagct ttcttcgccg atacgcgtgt tgcccgacag
    16621 gtgcgcgccc gggctgatgt ggacgaaagc atcaagcagg caatcgtgat cgacggtggc
    16681 ggcagtgttc acaattacgc cgtctttcaa tacgctgcca gcctgtacga cggctttcgc
    16741 cataacgacg ctgccttgtc cgactgttgc agaaggcgag acggtcgcgt cgggatgaac
    16801 caggacgggc agggcgaagc cgagcgcggc ggctttttcg gcgatttggc ggcggatgtg
    16861 gttgttgccg acggcgacgg cgatgtcgaa ttgttcgggc gataaactgt tttcaagcag
    16921 cagcgtcgtg ccgatgacgg ggaagccgtt gacgctgcct tggacgcggt cgtccagaaa
    16981 aacgatttcg ccgtatgtgc cgagtgcggc ggcaagctcg gcaacgactt tgccgtgtcc
    17041 gcccgcaccg acgacggcaa gtttgcgttt tcctgtgaaa gggggcatgg tggcttcgcc
    17101 ctgtgcggaa atcccttctt tgattaatac ttttttaacc gtcagcagta ggattttgat
    17161 gtcgaggcac aggctgaagt ggtcgatata ccagatgtcg catgcgaagc gttcgtccca
    17221 tgaagtggca ttgcgcccgt tgacctgcgc ccagccggta atgccgggtt tcacttcgtg
    17281 gcggcggttt tggaagttgt cgtacagcgg cagatattgc atcagcagcg ggcgcgggcc
    17341 gaccaggctc atgtcgcctt tgaggacgtt ccacagttcg ggcagttcgt ccaggctggc
    17401 ggcacgcagt tttttgccga aatcggtcag gcgttcgctg tccggcagcg ggttgccgta
    17461 tttatcgacc gcatcgcgca tagagcggaa tttaatcatt ttaaacggtt tgccgccttt
    17521 gcccggccgc tcttggatga agaacacggg cgagcctaga tttttgcgga tgaggtacgc
    17581 caaaatcagg aaaacgggtg agaggacaat cagccccgat gcggatgcga tgatgtcgaa
    17641 caggcgtttg aaaaatttat tcatcagcta atctttcaat caggttggcg attttttgat
    17701 aggcaacgtc gcgcctgaag cggcggacga tttcgtcgga ctgaacgggg tcgtctttgc
    17761 gtttcaaaat atttttggcg gcttgaacga agctgtccac atcgccggaa cggtagtttt
    17821 cgtgcggcag cagattcatg aggtcgagga cttcggcatt ggtctggctg ttcagcacgg
    17881 gtttttgcaa agccatatag tcggagagtt tgttggtaac cgactgcatg gcgtggctat
    17941 ggatggcgtt gacggcaatg tcgcagcctt tggcaatcga catcatttca gaatagggta
    18001 aataaccgta aaacttgatg gcgcgatttt catattgttt gagtttctcc aaatcgggtc
    18061 ctccgcccat aatgtgcaat tcgacgtttt cgccgtcgtc taaaagtttc cgaacacctt
    18121 tgcacacggt ttccacgtcg tagctgtaac tgagcgttcc caagtagaaa aggcgggtgg
    18181 ttttggagcg gaatcgcggg ggggggggcg atggcggcaa aatccgtacc gatatagacg
    18241 gcttcgccgg gtacgttcgg attggcttct ttggcgcggt cgagataagt ctgcgatacg
    18301 gcaaccagcg cgtcggcgca gcggtaggca cggttggcgc gtgaagcaaa gggcagcagc
    18361 ttgtgcggta cttttttcaa caatggtacg accgaggaga aagactccgg ccatacgtcc
    18421 tgcacatcga caatcagttt gtagcccaaa cgcgctttgt gtttgcccaa cagcaggttg
    18481 gtcgccatca gcggataggc agaatagacg atgtcctgtt ccgccgcctg cgggctgtgc
    18541 agccagcgtg ccaaattgcg gacgaatacg cggtggctgg cgacgcgggc gagggagacg
    18601 ttttttcggt agccgggttc gtccagcagc ttgacgcgca gcctgccgtt tgaagtggcg
    18661 gcagcatctt catgccgtct gaaggatttg tcgtagtggc ggaaacggct ggtaatcagc
    18721 aacacgtcgt gcgattgcga caatcgttct gccagatacc aaaagcggtt gaaatacggt
    18781 tcggacggca gcgagcaata aggggcgact atggtgatgt tcatcggcgg ctttcagacg
    18841 gcattacatg gtatacgccg ccatggtggt acggtagatt tcgtcgtcgg aacagagtgc
    18901 ggcgacgtgt gcgtgcagcc ttttgcccat tgcttcacgc aacagcggct gtctgacgag
    18961 cgtatctacg gcggcgataa aggcttcttc gtcgccgaag gggatgcagt aacccgtttc
    19021 cccgttgacg accatttcgg aaatgcccgc catatcgtag gtaacgacgg gtgtgtcgta
    19081 aagtccggct tctaagatgt tgttgcccac acctgcgccg tggtcgccga tgcagtgggg
    19141 cgtgttcacc aaaatatcca cgtctttgaa gtaggcggtc aaatcgcgga tgccgccgag
    19201 gaaggtgact ttgtcttcaa tacccaaacg tttggcttgg gctttgaggt tgtccatttc
    19261 ttcgcctatg cccgccacgt tcaggcggac gggcataccg cggtcaacca tttttttgaa
    19321 aatatccaac atcagatgca cggcgcggac ggtgtccaaa cgggaaagcg tgccgagtac
    19381 ggcgcactct ttttcggttt tccggaaatg aaattccggg ggggggggga gtgcgttgta
    19441 ggcgtaggca atccggccgg cggggaaacc gtggcggatg agtttttcgc gctcgtgttt
    19501 gcagttaccg atgatgtaca cgcccagctt gtcgaagagt ttggcgattt tggggtaggt
    19561 tgccgcgtcc aaaccgtgcg agtggtagat gattttggtt ttcggcgaaa cgattttggc
    19621 ggcaatggcg caggcgggta cgacgcgcgc catttggcag tcgatgatgt cgggttgttc
    19681 gcgccgcagc attcgggcgt aggcaatcat acctttgagg tagccgaaaa ggccgccgcg
    19741 atagaaatcg acgggttgcc aacgtatgcc caaggcttgc gcctctttga gcaacggtcc
    19801 gtccgaagag gcaagcatga tgtcgtgtcc gtgctgcctg agtagcctgc cgaggcggac
    19861 ggtggcggtt tccgtgccgc ccaagcctgc catagatgtg gtcaaaacga ttttcataat
    19921 gggaaacctt gttttttcaa ataatgaaac agtttgtgca aatctttccg gtggcgcagg
    19981 atgcagcctg ccagatatgc cgcccatatg ccggcaaaca gggggtagtt tgccggcgtg
    20041 ccgaagcagg tgtaggccgc cgaggaggcc aggcagaaca atgtgtgcat ataaagcggc
    20101 aggcgtttga gcggctgcca caggcggcag gagctttcgg tcttgaaaac aaaaaacagc
    20161 caaaatgagg cggcacaggc aaccgccgcg ccgcgcgcgc cgccggacgg tacggcaagc
    20221 cccagcagca gcaggtttgc cgccagcgcg cccaaggtgg cgagcgcgat cgggcgtgtt
    20281 tttcggacga cgttcaaacc gatgccgctg atttctacca gcgtgcaaaa cagcggcggc
    20341 agcatacacg atacgacgat aaaccggacg gcggcgtagt tttccggcag caggagggag
    20401 gcgaggggcg agaaaatgcc ggtcaggcag agggcggagg caagcagggc ggcggcggat
    20461 tctgccgttg ccgagaggcg ggcgggcggg gcgtttgctt cgattgcgcg gaaaatatac
    20521 ggtgtccaga ccgttgaaaa gatgctttgg aacaataatg ccgctccgcc gaacgaaata
    20581 cccatcgaat aaacgccgag ctgttctagg ccggcatatt ttttcaggaa caaacggtcg
    20641 gcggatgcca gcccccaata ggcgatgctg cttagtgcga tcggtatgcc gtagcgcagg
    20701 ccgcgatgca ggacggcgga tgaaaacggt gcgcgccgga cggccttcag acggcatcgg
    20761 ttttgaaaca gcaaaaaggc ggcggcggca aggtttgcca gcgcgtaaac ggcggtcagg
    20821 acggcggtgt tcgccggaaa gtgcagcagc ccgaccgtca gcggcagcag cagcaggatg
    20881 gcgagcttgg acacgagttg cgcggacgaa aaggcaaggg cgcgtccttc catacgcaaa
    20941 accagtaaga gaaagcggat gggcaggaag ctcagttcaa acagcaccag cccgatgccg
    21001 gcggcggcat cgtcgagcga aaacaggatt tcagacggca gggacgggcg ggaaagcagc
    21061 agggcggcta tcgcggcggc agacagcagc ggcggcagga acagggtttt gaacaaggtg
    21121 tctttgtcgg cggtggcata gtattcgcgg acgtatgcct gatccagccc gaggcacaac
    21181 accgacaccg tcagtcccgc cgccgtctgc atcagcacga tgcgcccgat gtcgtcggcg
    21241 gggaaatacc acgacagcag cggcaggatg atgacggcta aaaccgcgct gccgatcgag
    21301 cctgccgcgt agccgaggat ttcttttgtg tccatttttg atgtccgggc ggcggcggga
    21361 tgctgcctgt gccgtctgaa gcctttcttg atcggaattt gacggctttc agtccgttgc
    21421 ggctgccggc ggggtgcggc agtccgggtt gcgcttttcc gggcgggcgg cggtctgaac
    21481 ggggccgttt tttatcggcg ttattatata gtgaaacggc gcaaaccctt taaaaggcgt
    21541 tatcgttttt ccggaacacg gttttgatgt cgtgtccgag gatttcgctt gaaacgggtg
    21601 tccacaaggg cggtgcggaa agggcggcgc ggttttcggg cagggaaagc aggtctttgc
    21661 cgctgccgag gattttgggc gaacggtaca gcacgatttc gtccgccaga tcttctgcca
    21721 gaaatgcgga tgtgagttcg gagcctgctt cgaccatgat ttctccgaaa ccttcgtcgg
    21781 caaggaggcg catcaggtgg ttcaggtcga ttttgccgcc tgccgtttca gacggcatca
    21841 ggatgcggat gtgtgcgtgt tcccgatagg ggtgcagtct gtcttcgttg cgttcgagtg
    21901 tggcgatgta ggttggagat tgtccgtcgg tcagaaggtg gctgtccaag ggcaggcgca
    21961 ggcggctgtc taacacgatg cgtgcgggtt ggcgcaaagt tggaaaagcg cggacgttga
    22021 gccggggatt gtccgccaac accgtgccga tgccggtcag caccgcgcag ctttcggcac
    22081 gcaaaacctg tacgtcggca cgcgcgtctt cgccggtaat ccaaaagctg ctgccgtctg
    22141 aaagggcggt tttgccgtcc agcgaaacgg cgcatttgag gcggacaaag gggcggcggc
    22201 gttcgatgct cgataaaaat ccccggttga gttcgcgtgc tttgttttca agcagtccgc
    22261 attctgtttt gatgcctgcc gattcgagca gggcaagccc tttgccggca accagcgggt
    22321 tggggtcgcg catggcggca acgacgcgtg ttacgccgga acggagcaga gcttcggcac
    22381 agggcggggt acgcccgtaa tggctgcacg gttcgagggt aacaaaggcg gtcgcgcctt
    22441 ttgccatttc ccccgcctga tgcagggcgt ggacttcggc gtgcggttcg cccgctttga
    22501 cgtggaagcc ctgcccgaca atttggctgc cgtgtgcgat aacgcagccg acgcgcggat
    22561 tgggcgaagt ggaaaaacgc cccaaagcgg caagtttgag ggcgttttgc atcatagaaa
    22621 tatctgtttc ggaaaacatg gggataccgt atcagtatgg gttgggggaa tcaggctttg
    22681 cggtctgttt tgacggcttg cgccagccac gaggcaaagt ctgccggatt gtcgaagcgt
    22741 ttgtgcaggg cggcgaaacg gacggcggct tccgtatctt gtcggagcag ttcttttagc
    22801 accatgtcgg caagtgcggc agaggggatg tcgcgctgac ccgaagtgta gagcctgtgt
    22861 tccgtcaggc ggacggtttc gtcgatctgt tcgggtgtca gggcggattt tcgggcggcg
    22921 gcggtcaggt cgttgcggag gcgttgtgca ttaaagggcg aacgtttttt gtccggaccg
    22981 atgacggcgg gcattttgag ttcggcggtt tcgagcgtgc cgaagcgttt gccgcagttg
    23041 gggcagtatc ggcggcggcg gacggcgttg cgttcgtcca tcaggcgcga atcggcaacg
    23101 cgggtgtcgg ggtgggcgca aaacgggcat ttcatcgggt tcgtcctcct atgtcgtctg
    23161 aagttcagac ggcgacgccg gcggcgcgcg cggtttccaa accttcttcg acactcatat
    23221 aaacggggtt ttcgggacgg tcgtgtcgga cgatgttgcc ttcgcggaac atgaccagtt
    23281 cgtccacggc aagttgggac catgattcgt cgcgggtcag cggcagggtg gcgataacgg
    23341 cgacgcggtc ggagggcgtg gttacttcgg caaaatcgac catcacgtcg tcgtcgagca
    23401 ggcgcgcctt gccgaacggg gcttggcgga cgatgtagtg cagcagtgtg ctggcgtggg
    23461 caaacagggc aatgccgtct gaaagcataa agttaaacag cccgaacttg cggatttcgt
    23521 gcgtcagccc cgcaatcgcg tcaaacagcg tgtcgtcgtc gggacgggcg gcaaagcggg
    23581 tgcgcaggcg gttgaggatg tggcagaacg cgcgttcgga atcggttgtg ccgacggggt
    23641 ggaaaaattc gccctgttcg gggaaaaaat caatcaaatg cccgttgtgg gcaaacagcc
    23701 agtagccacc ccacatttcg cgcataaagg gatgggtgtt cgccagcgag gtttgtcctt
    23761 gcgatgcttt gcggatatgg gcgacgacgt tttccgattt aatctggtag gcgcgcacga
    23821 ggtcggcgac gggcgaattg gcactgggtt tgtcgtcatg gaacaggcgc acgcctttgc
    23881 cttcgaaaaa gccgatgccg aaaccgtcgg catggtggtc ggtaatgccg cccctgcggc
    23941 ggaagccttc aaaggaaaac ataatatcgg tcggcgtatt gcagttcatg cccagcagtt
    24001 gacacatggt ttgtcccaat gattcagatg gtcgcaagta ttcggattat accccgaact
    24061 gaaaatgccg tctgaaatac ggcttgttcc ccgttattcc ctcgaaaaca gaaaacagaa
    24121 aacaaagacg gaaacttaag attccgtcat tcccgcgcag gcgggaatcc gacttgtccg
    24181 gttttggttg tttttcgttc cgtaactttt
//
LOCUS       DAWKOD010000012        23683 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000012 DAWKOD010000000
VERSION     DAWKOD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.012"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(220..2910)
                     /gene="glnE"
                     /locus_tag="WJK73_000361"
     CDS             complement(220..2910)
                     /gene="glnE"
                     /locus_tag="WJK73_000361"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ7556898.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVWTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFSYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRKLHRQIHS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQLLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPEEQTQSNSEWQWVWQDKPDEEERQGRLKEHGFDAETIATR
                     LDQIRHSHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALADEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIATDCGLIDKTLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3128..3310)
                     /locus_tag="WJK73_000362"
     CDS             complement(3128..3310)
                     /locus_tag="WJK73_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556899.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLMLF"
     gene            complement(3447..3920)
                     /locus_tag="WJK73_000363"
     CDS             complement(3447..3920)
                     /locus_tag="WJK73_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ7556900.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(3973..4254)
                     /locus_tag="WJK73_000364"
     CDS             complement(3973..4254)
                     /locus_tag="WJK73_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556901.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4341..4664)
                     /locus_tag="WJK73_000365"
     CDS             complement(4341..4664)
                     /locus_tag="WJK73_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556902.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(4997..6004)
                     /locus_tag="WJK73_000366"
     CDS             complement(4997..6004)
                     /locus_tag="WJK73_000366"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ7556903.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6236..6472
                     /gene="acpP"
                     /locus_tag="WJK73_000367"
     CDS             6236..6472
                     /gene="acpP"
                     /locus_tag="WJK73_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ7556904.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6628..7875
                     /gene="fabF"
                     /locus_tag="WJK73_000368"
     CDS             6628..7875
                     /gene="fabF"
                     /locus_tag="WJK73_000368"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ7556905.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8230..9366
                     /locus_tag="WJK73_000369"
     CDS             8230..9366
                     /locus_tag="WJK73_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7556906.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRARGGGGDLERLAARDIIRFPGFVNNVSEVIK
                     EHHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRIL
                     AEKMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
     gene            complement(9609..10460)
                     /locus_tag="WJK73_000370"
     CDS             complement(9609..10460)
                     /locus_tag="WJK73_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7556907.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
     gene            10809..12323
                     /gene="katA"
                     /locus_tag="WJK73_000371"
     CDS             10809..12323
                     /gene="katA"
                     /locus_tag="WJK73_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7556908.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            13435..13878
                     /locus_tag="WJK73_000372"
     CDS             13435..13878
                     /locus_tag="WJK73_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7556909.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSDNGVFPKNNASAGVASASEIVGKYVAKVDVSGNTITSTMKTSG
                     VNKDISGATLVLEGAQKSGSFTWTCKAGSSNGVKDKFLPSSCRAK"
     gene            14140..14640
                     /locus_tag="WJK73_000373"
     CDS             14140..14640
                     /locus_tag="WJK73_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7556910.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            15116..15682
                     /locus_tag="WJK73_000374"
     CDS             15116..15682
                     /locus_tag="WJK73_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ7556911.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(15958..17994)
                     /locus_tag="WJK73_000375"
     CDS             complement(15958..17994)
                     /locus_tag="WJK73_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ7556912.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFTLSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(18082..18954)
                     /locus_tag="WJK73_000376"
     CDS             complement(18082..18954)
                     /locus_tag="WJK73_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7556913.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            19224..21614
                     /gene="gyrB"
                     /locus_tag="WJK73_000377"
     CDS             19224..21614
                     /gene="gyrB"
                     /locus_tag="WJK73_000377"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ7556914.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            21713..23098
                     /locus_tag="WJK73_000378"
     CDS             21713..23098
                     /locus_tag="WJK73_000378"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ7556915.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(23169..23267)
                     /locus_tag="WJK73_000379"
     CDS             complement(23169..23267)
                     /locus_tag="WJK73_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ7556916.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(23312..>23683)
                     /locus_tag="WJK73_000380"
     CDS             complement(23312..>23683)
                     /locus_tag="WJK73_000380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556917.1"
                     /translation="VKEYQPEVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYDLVQTEYRRFKADKT
                     ENRNHKANSTFEYLHILEKTF"
BASE COUNT         5618 a         6386 c         6246 g         5433 t
ORIGIN      
        1 tcttatttgt ttgttttgat ttttgcttgg ataaaaaatc ccatcattcc cacaaaaaca
       61 gaagcctgaa atcccgtcat tcccgcgaaa gaggaatccg gtttttgggt ttcagccatt
      121 cccgataaat cgctttagct ctgccgcaac cttaaaacgg cacggtacga aaataccgtc
      181 tgaaacccag attgtcaggc ttcagacggc attttttatt caggcggttg ccgcttcttc
      241 gccgaacact tcccgccaca atttcctgac attgccgtaa tgtgccagca attcgccgct
      301 tacttcggtt ttttctgcgt cgcgcagttt ggtattgtgt tgctgccggc ggtagaagcg
      361 gtaggcggtg cggctttgtt ctgcaagggt tttgtcgatg aggccacagt cggtggcgat
      421 gttcaagagg gcgatgttgc cgtagttgtc caagagttgc ggatattttc gggcgtgggc
      481 aagtatcaga tattggacga taaattcgac atcaaccacg ccgccgcgtg cgtatttgac
      541 gttgctgtcg gctgggggat gagtggggaa cattttttcg cgcatttcga tgatttcgtc
      601 tgccaaggcg gtttggtcgc gttcggcggt aaggatttcg gtgcggatgc ggtcgaatgc
      661 cgtctgaatt tcgggtgtgc cgcagatgaa gcgggcgcgg gtaagggact ggtgttccca
      721 cgtccacgcg ttttcgcgct ggtatttttc aaaggcggcg atgctgtgcg cgaggaaacc
      781 tgcgtcgccg ttggggcgca ggcgcaggtc ggtttcgtag aggctgcctg cgccggtggc
      841 ggtggaaagc cagttggtca ggcggcgggc gaggcggctg tacacgtcgc ctgcttcggg
      901 atggggatcg tcgtaaagat agaccaagtc gaggtcggag gcgtagccga gttctttgcc
      961 gccgagcttg ccgtagccga cgacggcaaa ttgcggggtg tcgcggtgtt ttttgggcat
     1021 gtccgcccac gcgcacagca gggcggcggc gaggatggtg tcggcaaggg cggagagctg
     1081 gtcggagagg gattctaccg tccacagtcc ggcgaggtct tgaacggcga ggcggaagac
     1141 ttgggcgtgt tggaaatggc gcagggtgtc catttgcgct tcagtatcgc cgccgcaggc
     1201 tttgaggtcg tctgaaaggg cggcggcgag cgcctgccaa tcaaacgcgg tatccaaaag
     1261 ctgcgcgctg atgagttcgt ccaacaaaat cggatatttg ttcagatacg ccgccaccca
     1321 agaactttgg cccataatct gcgccagttg cgccaaggtt tgcggatgtt cgttgaggaa
     1381 ggcgagatag gcggatcggc ggctgatgtt ttcgagaaaa tccaacagcc gcatcaatgt
     1441 atcggtcggg ttgctttgcg ctgccgccgc ctgtacgaac agcggcacaa ccgcatcgaa
     1501 acgcggctgg gcgtgtgcgg agaggtggcg gtatttatgg ctgtggcgga tttggtcgag
     1561 ccgcgtggca atggtttcgg catcaaaccc gtgttctttc agacggcctt gccgttcttc
     1621 ttcgtcgggc ttgtcctgcc aaacccattg ccattcgctg ttgctttgcg tttgttcttc
     1681 gggttcgctc agaatttcgt tgaacaaccg attgacttgg ttccgatgtt gatttaaagc
     1741 attcaaaaaa tcggcgtaat catcaaatcc catactttcg gcaagcagtt gccgctgttc
     1801 gggcgaagcc ggcagggttt gggtttgccg gtcgtcccaa tattgcaaac gatgttcgac
     1861 atcgcgcaaa aagcggtagg cggcaagcag ggtttcgacg ttttcagacg gcataatgcc
     1921 cgattcggcg agtttttcca gcgtttcccg cgtgcctttc agttgcagcg cgcgcatttg
     1981 cccgccgcgt atcatctgga aaatctgggc gataaattcg acttcgcgga tgccgcccgc
     2041 gccgagtttg atgttgtccg ccatcccttt tttgccgact tcgctgtgga tttggcggtg
     2101 cagcttacgc atcgcctcat acgcgccgta atccagatat ttgcggaaca cgaaggggcg
     2161 caccagcgat ttgatgtcgt tcggatacgg cgtgaccacg cggcctttgc accacgcgta
     2221 gcgttcccat tcgcgcccct gcgtaatcaa atattgctcc agcgcggttt cgctcaatac
     2281 cagcgcgccc gaatcgccgt ccggccgcag ccgcatatcg acgcggaaca cctgcccgtc
     2341 ggcggtaatg tcattcagca acgcaatcag tttctgcccg actttggtga aaaattcctg
     2401 attgccgcgt tcgcgcctac cgtcggtatc gcccgattcg gggtagataa aaattaaatc
     2461 gatgtcggaa gacacgttca actcatagcc gcccgccttg cccatcgcca ccacgctcaa
     2521 atgctgcggc gatttggtat aacgcccgat cggcgtgccg tacatatcct gataataggc
     2581 gtatgaaaaa tccaatgcgg tattcacagc aaaatcggca aacagcgtaa tcgtgcgggt
     2641 tacttcgttc aaatcgctga tgcggttgat atcgcgcacg ataatctgcg acaccacata
     2701 acggcgcaac ccgcgcaact gccgcgccaa ttcttcctcg ttttcttccg cacggatttt
     2761 gtcccagtcg gcaaaggctt ggaaatccgc ttcggtccaa accttgtcca gcataggcag
     2821 gaaaatttcg ggcttgagtt tgccgttgtc gagctggcgg gcgaggaaga gggaatggcg
     2881 gcgggcggtg tcgaggcggt tgtcggacat ttcggattcc gtttgaaaaa atggcaggaa
     2941 gatgatggcg gattcacttc aaatcaggac agggcgaggg gatgccgtac cggtttaaag
     3001 tcaagccact atatcatagc aaccttcgat gccgtctgaa accttgcccc gacatccaga
     3061 cggatgcctg ccgataaaaa acagcggcaa atgccgccgt ttcctgttca cacctcgcca
     3121 cgctctttta aaatagcata agccgcactt ttctcccgtg tggaattttt aaaaaaccat
     3181 cactcttgac gatttcatgc agctcctcac ttgatttctc cgcatattcc attttcaacg
     3241 ccttatgctc attgccggct tcaatcattc cctctcccac tgcttttgcc acaccattcc
     3301 aaaaacccat aatcatgtcc tccaatcgtt tcaaataaat aaggtcgttg tttctgtcaa
     3361 gaaacaacga cagtttataa actgtcggaa aacaaaatgc cgtctgaagg ccggataatg
     3421 cttcagacgg catcggattg ccggttttaa ccctgccctc cgccgttgtt caaatagtgt
     3481 tcccagtgag aatataaccg ggcaatattt tcttcatagg tgtgggcaga aggaatataa
     3541 ccgtctttgg gcggcatata cctgacaaat gattctattt cttccggata atcaaaatct
     3601 gcataaattt tttcaacttc ccccaaagga tcctcaaatt cttcttttct atggaataca
     3661 tcgcttaaga ggacgtacag ccaattttta ggggaataat ccctcatttc cgaagccaaa
     3721 tcttccagaa caggctttaa ttcaagaata ttggatttgt tgattaaaga tagtttgaac
     3781 acacgttcat catgatctga aagcgtcatt tttcgggcat aagctgccac atcagcccaa
     3841 cccaagtatt tattcccata cccccacaga atatccttcc aagaaagacg gactttttgc
     3901 gcggttaaat caaggttcat tttttactcc gattgcctgc cccgtcattc cgccccttcc
     3961 gattgacgcc tgttacgaaa agtcttccaa atgacggctg attgcctctt caacagtcgg
     4021 atgccaatcc ataaatgaag cctgcaattc ctctaaaacc aactcattga ataaatcatc
     4081 caaattccgc caatccgttt tttcatccgg atagatgaaa tttgccataa cgacagtatg
     4141 tttgttttgg gaataatccg ataaggcgca aatcatcaga taccgccccg aatgctccag
     4201 ataaaaaatt cgtttttcca aaggaaaccg gttattccgt tgcccgtttt ccatttttca
     4261 tcctttttaa cgtatcggct tccgcgtcct tcaaattgag ttacctgcct tccgccaagc
     4321 aaacgcccgc gcccctttca tcatttgaat atccgtttga ccatatttac cagataacta
     4381 tcttctttat ataaatcaat cagcaaaggc acgatacccg gctcataaaa taattctgtc
     4441 gtatcagatg tttcttttaa cgcggaaatt atctccaaca gttcataccc gtatcgggga
     4501 taagcaaaca aataggggac tgcttcccgc aaacatgaaa atgcttcgta actttgcatt
     4561 tcccatatat gttcatcttt aaaatcactc aaataccatt catccaattc actttccgga
     4621 tcggatatca atcggataaa ttcatttagt tcgtccttaa acatatcaaa tccttaaata
     4681 taaaatcttt tgattatccg ccgtatcctt attttccaca tcatgaattt caacgccgtt
     4741 catattttgc gttttcccaa gccgtccgcc acagtataaa attatttttt gtatcaattg
     4801 cttatatatt taacaactta ccgccggact tgctttggct gcgtatcaag attgtccggt
     4861 tcgcggcggg cgtttgcttt gtttcaaaca gggcggcgcg taagggattt tcaaggggcg
     4921 tcaggcggaa aaaggaaatg aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca
     4981 ttttgggcgg atcgcgtcat cgcgccaaag ccttcaaaca ttctttgacc aatgccggac
     5041 ctttgtatat caatccgctg tacacctgga cggcagttgc gcccaagcgg attttctctg
     5101 ccgcatcctt gccttccata atgccgccta cgccgataat cggcagcttg ccgtctatgt
     5161 ggtctgccaa cagcttcaac acccgattac ttttttcatg aacgggcagc ccgctcaaac
     5221 cgccctgctc gcctgcgagc ggatggctgc cgagacttga tttgtcgatg gtggtattgg
     5281 tagcgatgat gccgtccatt tcgacggatt tgacaacgtg ggcgatgtct tcgatttgtg
     5341 cttcatccaa atcgggggcg attttgacgg cgagcggaac gtatttcccg tatacagagg
     5401 caagctgtgc ctgtttgttt ttcaaagcct caagcaatgc gctcaactcg tcgccacctt
     5461 gcagcgcgcg gaggtttttg gtgttgggcg aggaaatgtt gacggtaatg tagcttgcgt
     5521 gcgcgtaggc tttttcaagg cagattaaat aatcatcggc agcgttttcg atgggtgtaa
     5581 ccgcgttttt accgatgttg atgcccaata cgccactgaa tttacttttt tcgatgttgc
     5641 gtatcatggt gtcgataccg tggttgttga aacccatgcg gttgatgatg ccttggtgtt
     5701 cgggaacgcg gaagaggcgc ggctgcgggt tgccgggctg cggtttgggc gttaccgtgc
     5761 cgatttcgat gaaaccaaag ccgagcgcgc ccaatgcgtc gatgtattcg ccgtttttgt
     5821 cgagtccagc ggcaagtccg acagggttgg gcaaatccat acccatcaat tttacaggtt
     5881 tggtacggtt gccggttacg ggaatcagac ccaatttata aaccgtgtag agcgcgtcga
     5941 gcgtgaagtg gtgggctttt tcggcatcga gtgcaaacag gatgcggcgg gcaagtggat
     6001 acatgacagg gctttctgct tcagatggaa atgccgccat tttaaccgaa atcccgccaa
     6061 acatacggca cacaggtcaa acttgaacga caaaacccgc cgcacccaaa aatgtacaaa
     6121 tcggcaaata ttgatataat agtcattaga tagaaaactc tattaaaagt cggtacaata
     6181 ccaccgattt atttccctta aacaacctgc cgcaaggcat aaaggaacga ctgatatgtc
     6241 aaacatcgaa caacaagtta aaaaaattgt tgctgaacaa ctgggcgtaa acgaagccga
     6301 cgtgaaaaac gaatcttctt tccaagacga cttgggcgcg gattctttgg acaccgtgga
     6361 gctggttatg gctttggaag aagcattcgg ctgcgaaatc cccgacgaag atgccgaaaa
     6421 aatcaccacc gtccaactgg ctatcgacta catcaatgcc cacaacggct aaccggtcgt
     6481 cgcccgacac aacagcctct gctgcaccgc gcaatagagg cttttccctt atatggcaaa
     6541 ctgtttgaac ccctgccgca aattacagac ggcatctgca cgacagccgc atatgccccc
     6601 attccgacaa ccaacagcga ggttatcatg agtcagagaa gagtagtcat tacaggctta
     6661 ggtcaggttt cccctgtcgg caacactgtc gcagaggctt gggacaccct gctcgccggc
     6721 aaaagcggca tcggcgcgat tacccgcttt gacgcatccg acatcaacag ccgtgtcgcc
     6781 ggcgaagtgc gcggtttcga catcggacaa tacatcagcg cgaaagaagc gcgccggatg
     6841 gacgtattca tccactacgg cattgccgcc gcattgcagg caatcgccga ttcgggcttg
     6901 gacgatgtgg aaaacctcga caaagaccgc atcggcgtga acatcggttc cggcatcggc
     6961 ggactgccca gcatcgaggt caccggcaaa gccgtaatcg aaggcggcgc gcgcaaaatc
     7021 aaccctttct ttatccccgg ttcgctgatc aacctgattt ccggacacgt taccatcctc
     7081 aaaggctacc gcggcccgag ctacgggatg gtttccgcct gtaccaccgg cgcgcacgcc
     7141 atcggcaatt ccgcccgact gatcaaatac ggcgacgcgg acataatggt tgccggcggc
     7201 gcggaaggcg caatcagcac tttgggcgtg ggcggttttg ccgcgatgaa agccctctcc
     7261 acccgcaacg acgaccccgc caccgcttcc cgtccgtggg acaaaggccg cgacggcttc
     7321 gttatcggcg aaggcgcggg catattggtg ttggaagaat tggaacacgc caaaaaacgc
     7381 ggcgcgaaaa tctacgccga aatcgtcggc ttcggcatga gttccgatgc ttaccatatc
     7441 accgcgccga acgaagaagg ccccgccctt gccgttaccc gcgcgctgaa agatgccggc
     7501 atcaatcccg aagacgtgga ttacgtcaac gcgcacggca cgtccacccc cttgggcgat
     7561 gccaacgaaa ccaaagccct caaacgcgcg ttcggcgaac acgcctacaa aaccgtcgtc
     7621 agctcgacca aatccatgac cggccacctg ctcggcgcgg cgggcggcgt ggaggccgtg
     7681 tacagcattt tggcgataca cgacggcaaa atcccgccga ccatcaacat ttttgaacaa
     7741 gacgttgaag ccggctgcga tttggactac tgcgccaacg aagcgcgcga cgcggaaatc
     7801 gacgttgcca tttccaactc cttcggcttc ggcggcacca acggcacgct ggtcttcaaa
     7861 cgcttcaaag gctgattccg caaagtcgcc gccgacatcg aaatgccgtc tgaaaccgtt
     7921 tcagacggca ttttcaaaac accccgcccg ttaccggcac ttcgcggcac acaaggcagc
     7981 cttataatac gcaggcaaac ccaagccctg ccggattgcg ggacgaaacc ctgccgtagc
     8041 agaccgtatt tccaatcata tattcaaggt gtccgcctta tgtttcaaca cacaggacga
     8101 cacataaagc accgccctat gtgtcgtcct gatttggaag gggttacgcc ccctcccaaa
     8161 tagagtctga ttctaccgcc ccgaaggaca gatgtccgag tggcggggtt caaccgaaaa
     8221 ggaaacacga tgaaaatcgt ttttatcaca acagtcgcat ccagcattta cggtttccgc
     8281 gcccccgtca ttaaaaaatt aatcggcaaa aaccatcagg tgtatgcctt tgtatcggag
     8341 ttttccgata atgaattgga tattatcagg gaaatggggg ttacacccgt tacctaccgt
     8401 tcaaaccgca gcgggctgaa cccgttttcc gatataaaat ccaccttcct catctttaaa
     8461 gaactcaaaa aaatatcgcc ggatttggtt ttcccttatt tcgcaaaacc cgtgattttc
     8521 ggcacttttg ccgcaaaact ggcaggcgtg cccagaatcg tcgggatgct ggaaggtttg
     8581 ggattcgcat ttaccccgca gccggaaggc ataccgttaa aaacaaaaat cataaagggg
     8641 attttgattg ccttgtacca cattgccctg ccgatgttgg aaagcctgat tgtattaaac
     8701 cccgacgaca aagacgaact gacggacaaa tacggcatca aaataaaaaa catccatatt
     8761 ttgggcggaa tcggtctgga tttgcggcaa tatccttatt ccgaggcgga tattcccgat
     8821 gaaaaagaac ccgtaaaatt cctctttatc ggcagatttc tgaaagaaaa ggggattgat
     8881 gattttattc gggcggcgga acaggttaag gacaaatacc ccgatacggt ttttaccgct
     8941 ttgggcgcaa tcgacaaatc acgcgcgcgg gggggggggg gcgatttgga acgccttgcc
     9001 gcccgcgata ttatccgttt ccccggtttt gtgaacaatg tttccgaagt gataaaagaa
     9061 catcatatat tcgtattgcc gtcttattat agggaaggcg ttccccgaag cactcaggag
     9121 gcaatggccg tcggcagggc agtgattacg acggatgtcc ccggatgcag ggaaacggtc
     9181 gccgacaagg tcaacggctt cctgatcgag ccttggaatc cccgcatctt ggccgaaaaa
     9241 atgatttatt ttatcgaaaa cagggaagcc gtccgcctga tggggaatgc aagttatgcg
     9301 attgccaaag ataaattcga tgccgaaaaa gtcgatttga aattactcga tattttgaag
     9361 gcgtaaacaa ggctgcccgc ttttgagttt cggtcatttc tgataaaccc ccgtcattcc
     9421 cgcgcaggcg ggaatccagg ttcgtttagt ttcggtcatt tccgataaat tcttgcagct
     9481 ttgcgtttct agattccctc tttcgcggga atgacgggat gcaggtttcc gcgcggacgg
     9541 attcggcatt cccgctttcg cagaaatgcc ggcttttggt ttgttgtctt tataagatta
     9601 ttcagcaatt attctgcggt tttgcgtttg tgtgctgccg gaatctcaag ggattctctg
     9661 tatttggcaa tggtgcggcg agaaacctcc atgccgcgga aagccagcag gttggcgagt
     9721 gcctcgtcag aatacggcgt gggtgtggac gaggcaaaac cggcggcggg aaagggattg
     9781 agcgaagcaa tgaggtcgag tgcggcttcg agtgtgccgc tgtcggtttg gggcaggtgt
     9841 ttttttattc gtgcgagggt ttggctgcgg ttgccgtcaa tgctgtcgag ggcgtttcgg
     9901 acgatatgca gggcggcggg tttggcagca cattcgccca atctttctat ctgcagtatc
     9961 agcgattcgt tcaaatcggc ggcggccacg cctgccgggt cgaatttttt caatgcggtc
    10021 agcgcgtgtt gcagcattgc ttcatccaac atccactcta agggcgtatg gtcgaggatg
    10081 tcttcgatgc tgtcggtcag ataaccctgc tcgtcaagga aatcgataag gatgtggaca
    10141 caggcggctt cttggtcgga aagcgggtgt tcgcatactt gcgcgtgcag gtattgcttg
    10201 aaatcctgct caccggcgat gttggacagc atatcttcgc cttcgtctcc gccgatttga
    10261 cgggcaggcg cggtgtaatg gctgaattcg gcatcggaaa attcatccgt gtctttgcgt
    10321 tcgagcaggg gattgtccga cagccagttt tcgacctcgc gttcaagttc gatacccgac
    10381 atctgcaata cgcgcaaaga ttgttgcagc cgctggttga gctgctgggt ctgtttgagc
    10441 tttattccga gtaaggtcat gataatgtgt tggaaattgt tattttcagc ctgtcggcgc
    10501 aaaaaaatgc cgcaaagcgt cattgcatta taaatggttt taatgagcgg gttcggattc
    10561 tgtctgataa caaaaaacaa acgaaaatca aaaaccgatt gcttataaca atattaaatc
    10621 gatttcatag ttttaatagc gaaaatcttg gcgtatagtc gcatccatag tttttacaaa
    10681 aggaaaacaa aatgtcgatt caagaaattt attgcaatca agaaaccggt tacgaatacg
    10741 ctttccgcca aatcgtactg taagccgttt acccccattt gaccaacctg agaaaaggaa
    10801 caagagcgat gactacctcc aaatgccctg taacccatct gaccatgaac aacggcgcgc
    10861 ctgttgccga caatcaaaac agcctgaccg ccggtcctcg cggccctctg ctggcgcagg
    10921 atttgtggct gaatgaaaaa ctcgccgact tcgtgcgcga agtcatcccc gaacgccgta
    10981 tgcacgccaa aggttcgggc gcgttcggta cgtttaccgt aacgcacgac atcaccaaat
    11041 acacccgcgc caaaatcttc agcgaagtcg gcaaaaaaac cgagatgttc gcccgtttca
    11101 ccaccgtggc aggcgaacgc ggcgcagccg atgcagaacg cgacatccgc ggttttgcct
    11161 tgaaatttta taccgaagaa ggcaactggg atgtggtcgg caacaacacg cccgtgttct
    11221 tcctgcgcga cccgcgtaag ttccccgacc tgaacaaagc cgtcaaacgc gacccgcgca
    11281 ccaatatgcg ctctgccaca aacaactggg acttctggac gctgctgccc gaagcactgc
    11341 accaagttac catcgtgatg agcgaccgcg gcatccccgc cggctaccgc catatgcacg
    11401 gcttcggttc gcatacctac agcttctgga acgaagcagg cgagcgtttt tgggtgaaat
    11461 tccatttccg cacccaacaa ggcattaaaa acctgaccaa cgaagaagcc gccaaaatca
    11521 tcgccgacga ccgcgaaagc catcagcgcg acttatacga agccatcgaa cgcggcgagt
    11581 ttccgaaatg gacgatgtac atccaagtca tgcctgaagc agacgcggaa aaagtacctt
    11641 atcatccgtt tgacttgacc aaagtttggc cgaaaaaaga ctatccgctg attgaagtgg
    11701 gcgaattcga gttgaaccgc aatcccgaaa acttcttcgc cgatgtggaa caatccgcct
    11761 tcgcaccgag caacctcgtt cccggtgtcg gcgccagccc agataaaatg ttgcaagccc
    11821 gtttgttcaa ttacgccgac gcgcaacgct accgcttggg cgtgaacttc cgccaaatcc
    11881 ctgtcaaccg tccgcgttgc cctgttcaca gcaaccagcg cgacggacaa ggacgcgccg
    11941 acggcaacta cggcagcctg ccgcactacg aacccaacag cttcggccaa tggcagcagc
    12001 agcccgactt cgccgaaccg cctttgaaaa tcaacggcga cgcggcacac tgggactacc
    12061 gccaagacga tgacgactat ttcagccaac cgcgcgccct gttcaacctg atgaacgacg
    12121 cgcagaaaca ggcattgttc ggcaacaccg ccgccgcaat gggcgacgcg cccgacttca
    12181 tcaaataccg ccatatccgc aactgctacc gttgcgatcc ggcatacggc gaaggcgtgg
    12241 caaaagccct cggactgact gtcgaagacg cacaagccgc ccgcgcgacc gaccccgcac
    12301 tcggccagcc cggtttgctg taagggggca ttatgtggat ggaaattgaa gaaatcctgt
    12361 cccgcgccgt ccgctaccgg aaataaccgg gcataaaaat gccgtctgaa acgtcgtttg
    12421 accgcttcag acggcatttc cacaccccac cccgcatttt tatatggtcg cgcatcgcag
    12481 tgggacgtag ggtgttcgta gggtgggctt cagcccacca atcacaccaa tcacaccaat
    12541 cacaccaatc cgccacctcg acaaatccgc cacctcgaca aatccgacaa atccgacaat
    12601 cttaccggtg ccggcaaacc tatgccgtct gaagttggcg gaggcggtgg gctgaagccc
    12661 accctacagc ccgccattcc cgccccacag ccaacttttc cgcgtcattc ccgcgaaagc
    12721 gggaatccag gttcgttgag tttcagccat ttctaataaa ttcttgcaac tttgagtttc
    12781 tggattcccg cctgcgcggg aatgacgaaa tttcagtttt tgattttttg tttttgcggg
    12841 aatgacggga tttgagattg cgggcattta tcgggaaaac agcaacctct cctccgtcat
    12901 tcccacgaaa gtgggaatcc aggttcgttg agtttcagtc atttctgata aattcttgca
    12961 gctttgagtt tctagattcc cactttcgtg ggaatgacgg gatacgggtt ttcgtgcgga
    13021 cagatccgtc attcccgcgc aggcgggaat ccggacccgt ccgcacggaa acttatattt
    13081 cgtcattccg ccaatcccac cccaccccca aaaaaaccaa aaaaaaccca atctgacaaa
    13141 atctgacaaa aaacgtcagc ccgaaactga caaaaattgt cacaattgac aaaaaaacgt
    13201 cagcccgccg caggccgcgc cgcgttgata taaaaaaata ccaataccaa acagacaaat
    13261 aaaaaaatat tctgtaaaat gccgagctga accccgtttt ggcacgccgc ttgctggaaa
    13321 gaaggcgaac cgaaacaaaa aagaccacgg tcaaatacat tacgggttta cgtttgcggc
    13381 ggatgccgca aatttccaat caaacttaaa cattttcaaa caggagtcat ccaaatgaaa
    13441 gcaatccaaa aaggtttcac tctgatcgag ctgatgatcg tcatcgccat cgtcggtatc
    13501 ttggcagccg tcgccctgcc cgcataccaa gactacaccg cgcgtgccca aatgtccgaa
    13561 gccctgactt tggcggaagg tcaaaaaacc gcagtggtcg agtattattc cgacaacggc
    13621 gtgttcccga aaaacaatgc ttctgcgggt gttgccagtg cttctgagat tgtaggtaag
    13681 tatgtggcta aggtagacgt ttcgggtaat accattacgt ctacaatgaa aacttccggt
    13741 gtaaacaaag atatcagcgg agcaaccctt gtactggaag gcgcgcaaaa atctggctcg
    13801 ttcacgtgga cttgtaaagc gggttcttca aatggtgtga aagacaaatt cctgccgtct
    13861 tcctgccgcg ccaaataagg acaatgaccg ggtctgaccc ggccgtgaaa acaaatgccg
    13921 cccgaaccgc tcgggcggca ttttctattc ccctttgccg ctgtagggtg ggcttcagcc
    13981 caccctacga aacaccctac gaaacaccct acagcctaca cacccgccct acgaaacgcc
    14041 ctacccattt ccctgcaaat cccctaaaaa tccgcccgca cgccctgaaa acaaggaata
    14101 atacaggttt ccgcccatcg ggctaaaaag gggataaata tgtcaaatta ccggagggac
    14161 ttcacgcgcg gggcgcacta ttttttcaca ttggctttgc aagacaggac gaaggattac
    14221 ctgatacggc atatcgacag gctgcgcctc gcctatcgtg aaacgaaaac acgttatccg
    14281 tttgaaacgg cggcaatttg tattttgccg gatcacctgc atttcattat gcggctgccg
    14341 gaaggcgacc acgattaccc gatacggatt gcatatttga aaacccggtt ttcccaactc
    14401 atccccaaag agtgccgcgc ccccaacggg agtaaaacaa aaaaacgcga agccggcatt
    14461 tggcagcgca ggttttggga acacctgatc cgggacgaac aagatttaaa caaccaccgg
    14521 gattatattt atttcaaccc cgtgaaacac gggtatgcgg cgagtgtgaa ggaatggcgt
    14581 tattcttcct tcttcaaaga cgttgaaaaa ggcatttacc ctttggattg gggagggtaa
    14641 agctcccgcc gccgccaaaa tccgcaggac ggacgcgggg cagatgagtc gggcgggacg
    14701 tagggtgagg cgtaggtgcg tagggtgggc tgtagggtgg atgtgtaggg tgggcttcag
    14761 cccaccaatc caaccacctc cgccaatcca accattccca ccaacccaaa ccaaccattc
    14821 cgccaatccc cgccaaaaac cgaaaccgcc gaatcccgac ggtttgccaa aaacccttga
    14881 tgcggtggaa ttggcgggat tggatgagtt ggtttggcag aattggtggg ctgaagccca
    14941 ccctacagcc caccctacaa cccgccctaa gtcccaccta cgttccatcc gacgcatccg
    15001 ccctacggtt ccggattccc ctgcgaaccg ccccgcgcac gaaaactgct ttacgcggcg
    15061 tttttcgcgt tttgctgttt tgctataatc cgcccttttt aaggacgggt gcggtatgag
    15121 tctttacgct ttgctcttga ttgcgctggg gatgtcgatg gatgcgtttg ccgtcgcgct
    15181 ggcaaagggt gcggcggtca ggatgcctcc gcgcaaaatt gcagcaacag ccttggtgtt
    15241 cggcacggtt gaagcgctca cgccgctggc aggctgggta ggcggttttt atgccaagcc
    15301 gtttatcagc gaatgggacc attgggcggc tttcgtcctg ctgggcgggc tgggtctgaa
    15361 aatgatgcac gaagggttgt ccggcgaggc ggaggacgcg cgggaaaaca aacgggaaag
    15421 ctggtggttg acggttctga ctgcttttgg aaccagtatt gattccatga tagtcggggt
    15481 gggcttggcg tttatggagg taaacatcgc ctttgccgcc gcaatcatcg gtatggcgac
    15541 gacggtgctg gtggcggtcg ggctgacggt gggaagggct ttgggcgtat tgttcggcag
    15601 gcgtgcggaa tttgccggag gtttggtgtt gattgccatc ggtacatgga cgctcttgtc
    15661 gcacttgggg ctgatcgggt aaggactgcc aaaaaatgct gtctgaagcg ttgtttgacc
    15721 gtttcagacg gcatttttta ttttttgggc gtagggcgaa tccgtaaggt gggatgtaag
    15781 gtgggatgta gggtgggctt cagcccaccc tacgcccact gtttttgatt ttttgttttt
    15841 gcgggaatga cgggattttg gcttactgtt tttgtgaaaa tggcgaaacc gcgcaaatgc
    15901 tgatgccgtc tgaaacactg tttgattgtt tcagacggca ttttttgttc acatcgatca
    15961 agccgcgttg tcgaaaccgc tgtggcgcaa gagtgcgtct atgctcggtt cgcggccgcg
    16021 gaaggctttg aaggattctg ccgcgctgcg cgatccgccg acggcgagga tttcctgcca
    16081 aaagcgtttg cctgtggcgg cgacatcgtc gctttcttca aaggcggcgt atgcgtccgc
    16141 gctcaatact tccgcccacg cgtagctgta atagcctgcg gaatagccgc ctgcgaagat
    16201 gtggccgaag ctcaaggtga agcggttgta ttcgggcggc tggatgacgg cgactttttt
    16261 gcgcacgctg tctaaaacct gttgccagtt tttcagacgg ccttcgtcgt cttcgctgta
    16321 aatcatcata tcaaagaggg cgaactccat ttgccggacg aggaacatgc cgcgttggaa
    16381 gtttttggcg gcgagcattt tgtcgaagag ttctttcggc aggggaacgc cggtttcttc
    16441 gtgggctgac atttgtgcca agacattgta ttcccaaacg aaattttcca taaactggct
    16501 gggcagttcg accgcgtccc attctacgcc gttgatgccg gatacgccca gttcgtccac
    16561 ttgggtaagc aggtggtgca gcccgtgtcc ggtttcgtgg aagaggatga ggatttcgtc
    16621 gtggctcagg cgggcttccc tgccgccgac gggtggggcg aagttgcaga cgaggtaggc
    16681 ggtgggcagt tgcagcgtgc cgtctgaaaa acggcggcgg cctttgtagt cgttcatcca
    16741 cgcgccgccg cgcttgcctt cgcgggcgta gaggtccata tagacgccgc ctatggtttt
    16801 gccgttttgt tgcaattcaa aatagcgcac gtctttgtgc cagacgggga cggttttttc
    16861 ggtaaatccg atgccgtaga gttttttgat ttgggcgaac agtccgttta atactttgcc
    16921 gacggggaag tattttttga cttcggtttc gctgaacgcg tatttggctt cgcgcagttt
    16981 ttcgctggcg tagcccaagt cccacggttg caaatcggcg aggccgaggc tttcgcgggc
    17041 gaaggctttg acttcggcga ggtctttttc ggcgtagggt ttggcgcggc gggcgaggtc
    17101 gtgcaggaag tttaaaactt gttcgggggt gtccgccatt ttggttgcca gcgacaattc
    17161 ggcgtagttt ttgaagccga gcagtttggc ggtttgcagg gcgtttgcga gcgtgcggtc
    17221 gatgttggcg gtgttgtcga atttgccgtc gtctgacagt tcgctggcgc gggtaacgta
    17281 ggcgcggtag atttgttcgc gcagttcgcg gttgtcggcg tattggatga cggcgaggta
    17341 gtgtggaatc tgcaagccga ttttgtagcc tgttttgctt tcgctttgcg cggcggcggc
    17401 aaacatggcg agcgcgtctt cgggaatgcc ggcaagcggt gcggcatcgt caaagtaaat
    17461 gccgaacgcg tcggtcgcgt ctaggacgtt ttgggagaat ttggcggaaa gttgcgcgcc
    17521 ttcggtttgc agttttgcca gttctgcctg ctgttcgggc ggcagttccg cgccgctgag
    17581 gacgaaatcg cgcagatcgt ggttgagttt ggttttttgt gcgggggaga gggtgtcgaa
    17641 ttcgggggaa tttttgatgg ttttgaagcg gttgtacagc tcgatgtctt gtccgatttc
    17701 ggtgaagaag acggtgattt cgggcatcag ttcgttatag acggcgcgca gttcgggcgt
    17761 gtcggcgacg gagttgaggt gcgacaccac gccccaaatc ctgccgacgc gttcggtgat
    17821 gccggtcagg ggttcgacag tgtttgccca gccggtgtgc gtttgggctt tgatggcggc
    17881 gatttgttcg cgcgcttcgg cgatggcggt ttgcagggcg ggtttgatgt cttcggtttt
    17941 gatttgatca aaacggggtt cttcgcccaa atggagcagt gcgttgtcag tcataagatg
    18001 ggtttccttt gcgtgttgtg cttcagacag catatcggca atatgccgtc tgaagccgga
    18061 aatggggtcg gtcggggaaa gttaaagtgt ttcggagccg gcggggtaat ttaacaggcg
    18121 gtattgtacg cgcaaaaatt cgtccataac cgcctgttgg atttccagcc gctttgaaac
    18181 gggggaggcg aagcggacga tgatgcggta tgccttgtca tcgtacggca cgcgggtaac
    18241 gcgcggtctg gcggcgggcg tgataaacag tttttccgcc tgcacgtttt ccaaatgccg
    18301 ttggatggcg gggatgtagg gcgcgcacaa gggctcgagt acggctttca gacggcatac
    18361 ggcttcatcc gaatccaaat ggatggggac ggggatttcg accgtatgga tgacatagtc
    18421 gcccaaaata ttgtcgcggc gcacggggtg gctcaacaac aggctgttgg ggaaagaaac
    18481 ggtggttccc gcaagctgtc cgaccaaggg gttcggaccg acctgcatca tcagcgtgtt
    18541 caacaggttg atgtcgacca cgcgcccgcg caggccgttg atttcgatat agtcgccgac
    18601 cgagtattgc tgggtggcag accttaaaat actgcccgac agacacataa tcagttcctt
    18661 cgtcgccacg acgaccgccg ccgccaccgc aaacatcgac aaagccagcg tttggatttg
    18721 cgccgaccag ataaatgcca gcgaaaacag caccaaaagc agcgttatat tgcggctggc
    18781 aaccaaaaac cgccgcttgc tttcgatgcc gaaatccgga tgccgtttga agtggatatt
    18841 caacagaagg gcgcgcgcca gcagcaaagc cgcaaccgcc gccacggatt cgaccgcctc
    18901 cgcacgtatc gggacggcac cgagccaagt gtccaaccta ttccatattt ccatttccgc
    18961 gcccctgtcc gaaaagtata gggtgtagat tttagtggca aaaaaacgct tttgccactt
    19021 tacgggaaaa tccccgacct gatgccggag gcaaacgtac cgtttcatct tttcccggcg
    19081 gcacactgat gcggataacg ctgaattagg cggcaaaatc ggctataata cacaattata
    19141 tatgccgtcc cgcccgcttt ttccacaagg tgcggacttg ttttggcggc atcgcaaaat
    19201 cttttcaaaa tccggcaaaa aatatgactg aacaaaaaca cgaagaatac ggcgccgaca
    19261 gcattcaggt gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg tacatcggcg
    19321 acacgcagga cggcagcggc ctgcaccata tggtgttcga agtattggac aacgccattg
    19381 acgaagcact cgccggacat tgcgacaaaa tcacggtaac gatacacgcc gaccattccg
    19441 tcagcgtcgc cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa gaaggacgct
    19501 ccgccgccga agtcatcatg accgtattgc acgcgggcgg taaattcgac aacaacagct
    19561 acaaaatctc cggcggcctg cacggcgtgg gcgtgtccgt cgtcaacgcg ctgtccgact
    19621 gggtaacgct gaccatctac cgcgacggca aagaacactt cgtccgcttc gtgcgcggcg
    19681 aaaccgaaga gccgctgaac gttgtcggcg attccgataa aaaaggcacg accgtgcgct
    19741 tcctcgccag tacggaaacc ttcggcaacg tcgaatacag cttcgacatc cttgccaaac
    19801 gcatccgcga actttcgttc ctgaacaacg gcgtggacat cgaattgacc gacgagcgcg
    19861 acggcaaaca cgaaagcttc gccctttccg gcggcgtggc tggattcgtg caatacatga
    19921 accgcaaaaa aacgcccttg cacgaaaaaa tcttctacgc gttcggcgag aaagacggca
    19981 tgagcgtcga atgcgcaatg caatggaacg acagctatca ggaaagcgtg cagtgcttca
    20041 ccaacaacat ccctcagcgc gacggcggta cgcacctgac cgcgttgcgc caagtgatga
    20101 cgcgcaccat caacagctac atcgaagcca acgaagtcgc caaaaaagcc aaagtggaaa
    20161 ccgccggcga cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa ctgcccgacc
    20221 ccaaattttc gtcccaaacc aaagacaaac tggtttccgg cgaaatcggt cccgtcgtca
    20281 acgaagtcat cagccaagcc ctgaccgact tcctcgaaga aaaccctgcc gaagccaaaa
    20341 tcatcaccgg caaaatcgtc gatgccgccc gcgcgcgcga agccgcccgc aaagcccgcg
    20401 aaatcacccg ccgcaaaggc gtaatggacg gcttggggct acccggcaaa ctcgccgact
    20461 gccaagaaaa agaccccgca ttgtcagaac tttatttggt cgagggcgat tctgcaggcg
    20521 gttccgccat gcaggggcgc gaccgcaaat tccaagcgat tttgccgctt aagggcaaga
    20581 ttttgaacgt ggaaaaggcg cgctttgaaa aaatgctcgc cagtcaggaa gtcgccgctt
    20641 tgattactgc gttgggtgca ggtatcggca aggaagaatt caatgccgag aagctgcgtt
    20701 accaccgcat catcattatg accgatgccg atgtggacgg cgcgcacatc cgcaccctgc
    20761 ttttaacctt cttctaccgc caaatgcccg agctggtcga acgcggctac atctacattg
    20821 cccagccgcc tttatacaaa gccaaatacg gcaaacaaga acgatatctc aaagacgaat
    20881 tggaaaaaga ccaatggctg ctcggtttag ccttggaaaa agccaaaatc gtttcagaca
    20941 gccgcaccat cgaaggcgaa gagcttgcca ataccgccaa acaattcctg ttggccaaaa
    21001 ccgtcatcga acaggaaagc cgcttcgtag acgaactcgt cctgcgcgcc atgctacacg
    21061 cgtcgcccat tgatttgacg tcgtctgaaa acgccgataa agccgttgcc gaactttccg
    21121 gtctgcttga cgaaaaagaa gtcgccctcg aacgcatcga aggtcatgaa ggacaccggt
    21181 tcatcaaaat cacgcgcaag ctgcacggca atgtggtggt gagctacctc gagcctaagt
    21241 tcctcaacag caaagcctat caaaccctta cccaaaccgc cgccgcactt aaaggcatgg
    21301 tcggcaaagg ggcgaagctc tacaaaggcg acaacgaata cgacatcgac agctttgaag
    21361 gcgctttgga catcttgatg agcgttgccc aaaaaggtat gtccatccaa cgatacaaag
    21421 gcttgggcga gatgaacccc gaacagctgt gggaaaccac gatggatccc gccgtgcgcc
    21481 gcctgttgaa agtgcgcatc gaagatgcca ttgccgccga cgaagtattc gttaccctga
    21541 tgggcgacga ggtcgaaccg cgccgcgcct ttatcgaaaa caacgcgctg attgcccaaa
    21601 atatcgacgc ataagtgccg ttttaaaaaa ggagacgggc atcgtgccgc gtctcctttt
    21661 ggtttgtcaa acggaagcca tgccgtctga aaccgccttt cggagcaaaa ccatggtcag
    21721 catttttgat attttcaaaa tcggtatcgg gccttccagt tcgcatacgg tcggcccgat
    21781 gaaggcagcc gccgcctttg cggcaggttt ggatgcacag gctgttcgca tcgtcatcga
    21841 catttacggc tcgctcgccc tgaccggata cggacacggt acatttgacg cgctgatgct
    21901 cggtttggaa ggcagcctgc cgcacgacat cccgcttgtc ggcattcccg aacgcctcga
    21961 acgcatccgc acgcagcaca tcctccggct caacgggcaa gaaatccgct tcatccccga
    22021 ccgcgacctg aacatactcg gcaatcaagt gctgcccaaa caccccaacg gcctgcgttt
    22081 taccgcctat gcttcagacg gcacggtatt gaatgaacag gtttattatt cggtcggcgg
    22141 cggctttgtc gttaccgaag aagattttga ccggcaggcg gaaacggaaa aagccgttcc
    22201 ctatccctat accagttgcg ccgaactgct tgcccgatgc cgtctgaacc ggctcgacat
    22261 ctccgaagtc gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag ccgaaatccg
    22321 ccgccgcgcc gctgccgttg ccgaggttat ggaaggctgc atcaaacgcg gcttgggtgc
    22381 ggacggcgaa ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc ttgccgccaa
    22441 gctcaaagtc ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga tggtgtacgc
    22501 catggcggtc aacgaagaaa acgccgccgg cggacgcgtc gttaccgccc cgaccaacgg
    22561 ggcggcaggc atcattcccg ccgtattgca ctatttccgc aagttcaacc cccacgccac
    22621 acaggaacgc gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc tctacaagac
    22681 caacgcctcc atttccggtg cggatgtcgg ctgtcagggc gaagtcggcg tagcgtgttc
    22741 gatggcggcg ggcgcatacg ccgaagtcat cggcggcacg cccaaacaag tggaaaacgc
    22801 cgccgaaatg gcgatggaac accatttggg gctgacttgc gaccccgtcg gcggactggt
    22861 gcaaatcccc tgcatcgagc gcaacggcat cgccgccgaa aaagccctca aactcggcac
    22921 gctcgcgctt ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag tcatccgaac
    22981 catgctgcaa accggccggg atatgaaggc gacctacaaa gaaacctcgc ttgccggact
    23041 cgccgccacg ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg agtgctgagg
    23101 atggacagaa acgaaaatgc cgtctgaaaa cagtttttta gacggcattt catttatttc
    23161 ttgcggcttt accgtttcag tgccagcgtc aacacgcccg ccgtaaccag ccccaagcct
    23221 atccattcct gcgtgttcgg gcgttcgtct aagaaaacca ccgccatcag gaatgcaaac
    23281 cggcgttgtt tgcagcatcc ggcaagaatt tttaaaaggt cttttctaaa atatgcagat
    23341 attcgaatgt cgaatttgcc ttatggttac ggttttctgt tttatctgcc ttaaaccgcc
    23401 ggtattccgt ttgaaccaaa tcatatttgc cgaaacgctc aaaaatctcc cttacctgcc
    23461 cgacagacat caagccttcg ttgttgtagc tgagaaaaat atattgaaag tccgcatccc
    23521 taatcaaagc ctcaaatacc ggaaccacat ccgattttga gcaagtccgc gctaaaacac
    23581 atcgtacagc gcaaaaccgg atgggaatgg caaaatccgg aaacaaacat ttttcaaagc
    23641 atacaaacaa aaaaatacca acctccggtt ggtattcctt aac
//
LOCUS       DAWKOD010000013        22251 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000013 DAWKOD010000000
VERSION     DAWKOD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.013"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1034)
                     /locus_tag="WJK73_000381"
     CDS             complement(120..1034)
                     /locus_tag="WJK73_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7556918.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WJK73_000382"
     CDS             1290..2759
                     /locus_tag="WJK73_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ7556919.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVAAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAATVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FTGRVLIPVVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WJK73_000383"
     CDS             3011..3382
                     /locus_tag="WJK73_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556920.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WJK73_000384"
     CDS             3455..4864
                     /locus_tag="WJK73_000384"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ7556921.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLSYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLETLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WJK73_000385"
     CDS             4911..5705
                     /locus_tag="WJK73_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556922.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5893..6669
                     /locus_tag="WJK73_000386"
     CDS             5893..6669
                     /locus_tag="WJK73_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7556923.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7327..9798)
                     /locus_tag="WJK73_000387"
     CDS             complement(7327..9798)
                     /locus_tag="WJK73_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ7556924.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9795..10973)
                     /gene="hflX"
                     /locus_tag="WJK73_000388"
     CDS             complement(9795..10973)
                     /gene="hflX"
                     /locus_tag="WJK73_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ7556925.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10978..12258)
                     /locus_tag="WJK73_000389"
     CDS             complement(10978..12258)
                     /locus_tag="WJK73_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ7556926.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12411..13130)
                     /locus_tag="WJK73_000390"
     CDS             complement(12411..13130)
                     /locus_tag="WJK73_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ7556927.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13201..13878)
                     /gene="radC"
                     /locus_tag="WJK73_000391"
     CDS             complement(13201..13878)
                     /gene="radC"
                     /locus_tag="WJK73_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ7556928.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSRSVCSF
                     RQLGLMP"
     gene            complement(14001..15350)
                     /gene="gshA"
                     /locus_tag="WJK73_000392"
     CDS             complement(14001..15350)
                     /gene="gshA"
                     /locus_tag="WJK73_000392"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ7556929.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15610..17019
                     /gene="leuC"
                     /locus_tag="WJK73_000393"
     CDS             15610..17019
                     /gene="leuC"
                     /locus_tag="WJK73_000393"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ7556930.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17116..17370
                     /locus_tag="WJK73_000394"
     CDS             17116..17370
                     /locus_tag="WJK73_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556931.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17432..18073
                     /gene="leuD"
                     /locus_tag="WJK73_000395"
     CDS             17432..18073
                     /gene="leuD"
                     /locus_tag="WJK73_000395"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ7556932.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18243..19514
                     /locus_tag="WJK73_000396"
     CDS             18243..19514
                     /locus_tag="WJK73_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7556933.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19530..20261
                     /locus_tag="WJK73_000397"
     CDS             19530..20261
                     /locus_tag="WJK73_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7556934.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20295..21365
                     /gene="leuB"
                     /locus_tag="WJK73_000398"
     CDS             20295..21365
                     /gene="leuB"
                     /locus_tag="WJK73_000398"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ7556935.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21528..22091
                     /locus_tag="WJK73_000399"
     CDS             21528..22091
                     /locus_tag="WJK73_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ7556936.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5362 a         5856 c         5782 g         5251 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgaaagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctgct tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttctccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tgttgcctgt ttgggcttta
     1201 aatggggttg cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggcggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgct gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccac cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagcgcg caattaggtt
     2641 tcaccggacg cgtattgata cccgtagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggagca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgcc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 gtcttatccc gaattggcgt ttgaaatcat gcgcctgttt gttacggata ttccggaaga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttatcca acggccccac
     3781 tttggcattc aaagacatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggacattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acagatgggt ttgcctatcc gtcgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccccgccgc gctggaaggt ttggaaacat tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccttac
     4921 ctatgcagga aacccgtttt tccatactgc tggacgaatt ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggga gcaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcacg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggtccccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc cggaaaaatg
     5221 cccttatgtt cggcaatttg gaaggcatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aggctggaaa gtcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacggcagtt cctcagtcag gcgtttgcgc gaaatatgat gtcggacgga aaaaacatca
     5401 gctttttgga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcagatca gcctgccgaa atacaaaagc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatatgccgc
     5701 cgtaagccgc cgcccgcccc acgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt accccgaatc
     5821 gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgtgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggcccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaactctag ttgccggcga cctgaatcca ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca atcctcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga cgatttcgga
     6601 ctaaccgttt ctccgaaaac aggcgtgcgc ggcgattacc tgattgagcg cgcgtttgtg
     6661 gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gggtaaaaat tagaaatgca catttccgtc
     6781 attccaacac aggtgggaat ccggacttac ctgtttcatt atcgtttgaa aataaaagag
     6841 aaaccaaaca tatagattcc cgcttgcgcg ggaatgacga cggtgttttg ccgtagccaa
     6901 agcagaaaaa catccctatt gtcatttcat catgatatga caataggatg ttaaaaatta
     6961 attatcatta tatgattgat atataagaaa aactgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccaccgt catacacaga
     7081 accggtattt ggaaaaaaaa taatccatat tgacatcatt attccattta tcgcataaat
     7141 ccacttttta attttttttc ctcattaata taatctctta tttaataaaa tcaaatacct
     7201 agcaattcga tctcaaattt taaacgccaa cgtgtttgag cggtaacaat gcatacttta
     7261 agtttgtaat ccttcatcgt ctcatttttg ttttaatcga ctatacgtct aaagcaacgc
     7321 cgtccgtcat tgttcggacg gcattttttc agacggcatt ttcccccgtt tgtgaaggcg
     7381 cagggcgttg cccaaaaccg ccagcgaact gaagctcata cccagtgcgg ctatccacgg
     7441 ttggacatag cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc ccgcccatat
     7501 caggttttgc cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt
     7561 acgcaaatct tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc
     7621 cgctgcggcg gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc
     7681 tatcatcagc acttttttcc cttctttttg caaggctttg acgtattcca gtttgtcctc
     7741 gggcatggct tgggcgcggt agtgcgtgat acccagggcg cgggcggttt cggcaacggc
     7801 ggtttcgcgg tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca actgccacac
     7861 cgcctccgcc gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt ggaaaccgct
     7921 ttgactgccg aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt tacctgaaat
     7981 ttcggcgaca taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt tgacggtcag
     8041 ttgcgcgccc acgccttcgc cgatgcggtt gaggcgttgt ttaatagcaa tgtcggggac
     8101 gctgccgcct gaaatgcggc agttgaggat ggcgcgcgcg agcgggtgtt cggactgctg
     8161 ttccaaactt tgcgccactg ccagcacttg ggcttcggtc atgctgccga gcagttcaat
     8221 ccggcggacg gcgggattgc cttgggtcag cgtgccggtt ttgtcgaaaa tgatgtcggt
     8281 ggtttgggcg agggtttcga ttgcctgctt tccgccgatt aaaatacctt cgcgcgccag
     8341 cgtaccggta gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc aggggcaggt
     8401 aatgaccagc agggcgacgg taatccacaa tgcggtgtgc gcatcggcgt acaacgtcca
     8461 gccgatgaag accgggacgg caagcagtag ttcgccgaat atgaaagacg aggcgtattg
     8521 ttccgccaac tcggcggtgc gcggtttttg cgccaaggcg cggtcgagca agcggacgat
     8581 gtgcgacagt cgcgtgccgc cgccggtgcg gtcggtgcgt ataatcaggg ggctttgcgt
     8641 gttgagtgtg ccggcggtta ctttttcaga cggcattttg gcgacgggca ggctctcgcc
     8701 ggtcagcata gattcgttga cggcactgct tccttccagc accgtgccgt caacggggat
     8761 ggtttcgccc ggtttgacca gcacgatatc gcccgccttc aatttgacga cagctgcctc
     8821 gcaggtttcc tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag gaatcagctt
     8881 caccagcctt tcggcagcat cgcctgcctt acggcgggca atgtgttcca taaagcgtcc
     8941 gcccagcagg aaaaacagca gcatggctat ggattcgaaa tacatcccct gccccgcatt
     9001 ggtcgcaagg ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg caatcggcgt
     9061 atccatgccg gcgcggcggt ttttcaagtc gcgcagcgcg ccttgataaa acgggacggc
     9121 gcaatagaat acgacgggca gcaccattaa aaagccgccc caatggagga tttgcaggaa
     9181 atcgggttcg atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat
     9241 catccccagc ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt tttggttggc
     9301 ggcttcgatt ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc tgattttcaa
     9361 cagaatgtcg gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct
     9421 gtaattgagg tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt cgatcagcca
     9481 gacgcaggcg gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg tgccgccgtg
     9541 ggtttccaca aagtcggact ggacttcggg caggtcgtac aggcggattt ggtcgaggat
     9601 ttcttggggc ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt aataactgcc
     9661 caagcccgcg tcaataatgc tttgtgcgac cgcctgacag ccggcgcagc aggtttcgcg
     9721 gtcttcgttt tcgtaacgga cagtcaggtg gaggtgttcg ggaacatcca gcccgcagtg
     9781 gaaacaggtt tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc ggcacaagac
     9841 tcggcaatgg cttcgcgcag tgcgtctata ccggtatttt cagcaacgga aatgcggacg
     9901 gcggcaattt ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc agacggcagc
     9961 aggtcggttt tgttgtacac cttgatgcac ggaatatcgc cggcatggat ttctcgcagt
    10021 acgttttcca cgtcttcaat ctgctgtccg ctgttcggag cggcggcatc gacgacgtgc
    10081 agcagcacat cggcttgcgc ggtttcttcc agcgtggcgg aaaaggcgga aatcagtttg
    10141 tgcggcagat cgctgacgaa tccgacggta tcggtcagga tgatgctgca ttcgggactg
    10201 atgtacagcc gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt cgcatatatg
    10261 cccgacttgg tcagccggtt gaacaggctg gatttgccga cattggtata gccgaccagc
    10321 gcaaacgttt tgattgtgcc cgattcgcgg gacttgcggc gcagggcgcg ctgttttttg
    10381 aggttggcaa gctgtttttt caaggcgttg attcgatggg cgatcaatcg gcggtcggtt
    10441 tccagtttgg tttcgcccgg ccctttcatg ccgataccgc cgcgctggct ctgcagatgg
    10501 ccgtaaccgc gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc gacttgcagc
    10561 ctgccttcct gcgtgcgggc gcggcgggcg aaaatcgcca aaatcaaccc taccctgtcc
    10621 aatacgcggc attggaggat tttttccaaa ttgcgttcct gcgtgggcgt aagttcgtgg
    10681 ttgaatacga ccaaatcgat gccgtctgcg gcaactgctt ccgacagctc cgccgccttg
    10741 cccgtgccga caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc cacgcgcacg
    10801 gaatcgccgc ccgccgcttt gaccagctcg acagcttccg ccaatgccgt ctgaaaaccg
    10861 ttcagacggg tggcactgct gcccgtgcca tctttgtcca acattacgcc caccagcatg
    10921 acgcgttcgg gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt catgtattca
    10981 atcctgtaaa taaagggtta cgcctcgttc cgtttctcct tccggtgcaa gcgtgatgcg
    11041 gctgcccgcc attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc ttcctatgga
    11101 caggcggctg ccgggtcgct gccggtaggt ataacgggta cacgcttcca aacaccagcg
    11161 tccgcgccag tggatgaaat atttttcgcc ttttctaacc gcaagtgtct cgccgccgtt
    11221 tacctcaaaa taagggtaat cgggttttcg ggttctaaac tgccatttcg cctgcgctcg
    11281 ccgtccgttg cggacatagt cgaataccgc cgtataaagc gtgccgtatt ccaaaggctt
    11341 gagcggaaaa agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt tgccggcggt
    11401 taaaaccctg acggggcgga tttcgttttt accctgatac agcttgaaac ttttcatcgt
    11461 aattttgcct gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga tttcatattc
    11521 cggcacgggg tctggacgct cgccgtggaa ataaggcagt gcgccgctgc cgacgggata
    11581 ggctgtatag agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga ccgcaccgtt
    11641 atggcaggcg ttgcggtaat atttccgtcc tgcttccggc tgatttctgc cttgggcgca
    11701 atgccgctca aacctgccgt tgccctgatt gaatacgaga acggttttac cgttttcgcg
    11761 cacaaatgcc gctcctgcct catccgtatg gcggtcaagt agggaaaggc ggtggtagat
    11821 tgcgctcatt aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt cggacgattc
    11881 ggcggcttct tcttccgtgc tgatgttttc atgcacgccg ttgtagagat acccggcaag
    11941 gcgtgtgcgt tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat ggtgttcgcc
    12001 gtgtccgtct tcgggattga gcgtgaggta gcttgcgtgc ctgcgggcgg agttttccaa
    12061 aaccggcgcg tgtgccagct tgtgcaaacc gatttgggct cggatgcggt tgaggtagtg
    12121 cagcgcatcg ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt cgcccgcggg
    12181 cagggattgg ttttgggtat ggtagaaaac gcctgccgcc gaacccaaca ggagcagccg
    12241 aataaaaagg gatttcataa tgccgtcctc ccgacgcggc aacggggtgt tttatcaaaa
    12301 cggcaaactt gccaatgcgg tttccgattg cagattgcca aaccggcaaa cctttccccg
    12361 tgccgatgcc gaacccgatg ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc
    12421 acccggatgg gggcaggttc gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt
    12481 ttgatgggaa tgcgtccctg cgatttgcgg tgggcattgg aatcgcgcaa ggaataggcg
    12541 caaccgcaat attcctgctg gtagaagttt tcacgtttgc tgatttcaat catgcgcgcg
    12601 ctgccgccgc ctttgcgcca gttgaaatcc caatacacca catcatcgta aggcgcggcg
    12661 gcacggcggc cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa
    12721 ctggtaaaga cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc aaaacgcata
    12781 tcgaaacaca tggtgcagcg gatgccgcgt tccggctcaa actccatgcc tttggctttg
    12841 gcaaaccatt ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa aggaatgccg
    12901 aacttttccg caaagcgcac gttttcctct tttcggagca tatactcttt gtgcggatgg
    12961 atattgggat tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat cgcctccatc
    13021 acttcgccgc tgcacggggc gcaacaggaa tgcagcaaga cttttttatg tccgccgggc
    13081 gggacgagta tggggcggtc gatgtcggta acgatgcgtg ttgtgtccat agtttcaaat
    13141 agatgttctg tttggtgcaa acggctattt tatcagagcc gccgcatgtg aaacagagtg
    13201 tcagggcatc aacccgagct gcctgaacga gcagacgctc cgggaggtta cgataaaatg
    13261 gtcgagcagc gacacatcga ccagcgacat ggcctgtgcc agccgccttg tgaacatgat
    13321 gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgtgcaataa tcaggctgtc
    13381 ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac
    13441 cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc
    13501 gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg caacggtatc
    13561 cggatcggac aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc
    13621 cctgactacg gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt
    13681 gccgacctcc gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc
    13741 caaatcgacc gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc
    13801 atcactcaaa gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg
    13861 ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacaa
    13921 atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc
    13981 atctgtattt caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccagaagc
    14041 gacaggcgtg ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag
    14101 tctttttcag agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct
    14161 tcgggcgctt cttgggagtt gtcgccgttg ccggtgggga tgctgttgga cagcggaaca
    14221 aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa
    14281 aagccgccga tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt
    14341 aaggtttcat aagtataaat cccttcttgg acaatcactt cgctgacttc caaaccttct
    14401 ttgactttcg ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat
    14461 ttgacgctca tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt
    14521 ttgtcggtaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc
    14581 gcttccgcca acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca
    14641 aaataagggt taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact
    14701 tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc
    14761 ggcaaaacgg tttggctgat gcctttgagg atgtcgggaa tgccggcaga caaatcgttg
    14821 ttcaacaaaa ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc
    14881 aataaaggtt ccaacaggat tttgtcgccc aatgcagttt caaattcggt cggttcggtt
    14941 acttccggat tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg
    15001 agggcgtaaa tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat
    15061 acggatttcg ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc
    15121 gggataaagt tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac
    15181 gaaattttgt aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgtacg
    15241 ctccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca
    15301 aacgcctgaa gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg
    15361 cctctgtgtg ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt
    15421 attctttcgt atcgaatcat gctttagata taactgttta ctgaaatgga caatacgtct
    15481 aattttttcg ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt
    15541 tgcgcggtat aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag
    15601 aacaacatca tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc
    15661 gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc
    15721 agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagt
    15781 gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac
    15841 ccgatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc
    15901 gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa
    15961 ggcgcgaccc tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacacacggc
    16021 gcattcggcg cactggcgca cggcatcggc acttccgaag tcgagcacac gatggcgacc
    16081 cagtgcatta ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa
    16141 gcgggcgtta ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcaggc
    16201 ggtacaggct acgccatcga gtttggcggc gaagccatcc gcagcctttc tatggaaagc
    16261 cgcatgactt tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc
    16321 gaccaaacca ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg
    16381 gacaaagccg tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa
    16441 gaataccgtt tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa
    16501 atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa
    16561 cgcagcggta tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac
    16621 gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg
    16681 cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta
    16741 atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt
    16801 ttcatcgaag ccggttttga atggcgcgaa ccgggctgct ctatgtgcct cgccatgaat
    16861 gccgaccgcc tgaccccggg tcaacgttgt gcctctactt ccaaccgtaa ctttgaaggc
    16921 cgtcaaggca acggcggacg tacccacctc gtcagccctg ccatggcagc cgccgccgca
    16981 gtgaccggtc gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa
    17041 gaatttgacg cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa
    17101 ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc
    17161 gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca
    17221 caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac
    17281 aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc
    17341 ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc
    17401 agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat
    17461 cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt
    17521 aaaatccatc aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga
    17581 ccacggcgaa ccgggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa
    17641 ccagccgcgc tatcaaggcg cacaaatcct gttgacgcgt aaaaacttcg gttgcggctc
    17701 ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc
    17761 cagcttcgcc gacatcttct tcaacaactg ctacaaaaac ggccttttgc ctatcgtttt
    17821 gaccgaagaa caggttgacc ggcttttcaa agaagttgaa gccaacgaag gctatcagct
    17881 ctccatcgac cttgccgaac aaaccctgac cacccctggc ggcgaaacat tcacattcga
    17941 cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg gactgaccct
    18001 gcaacatgcc gatgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct
    18061 gtttaacggt taaaacctat accgtctgaa ggccgttcag acggcattcc tgataacata
    18121 taattgctgc aactaatgta aacagagggg ctataaattg ctttcatctt tcaagcgggc
    18181 ttagcaacgc tctaaattaa tgcaatacag ctctatttca aataaatttt tagattttta
    18241 atatgcaaca gattaaattt attgacttat tttccgggat gagcggtatc aggaaaggat
    18301 ttgaacaagc ctgtcggaaa cagtcggttg cttgcgaatg tgtttttacc tcagaaatca
    18361 aaccggcagc tttggaagta ttgaagcaaa actaccctga tgaagtgccg tatggagata
    18421 taacgaaaat tgaaacggga gatattcccg attttgacat cttgttggca ggcttccctt
    18481 gccaggcttt ttctttcgcc ggaaaaagat tgggctttga agatacgcgg ggaacgcttt
    18541 tctttgatgt ggcaaggatt ttaaaggcaa aaaaaccaaa aggttttatt ttggaaaatg
    18601 tggaaggatt ggtgacgcac gatagaaaag atccgacaca aaaaatagga cgcaccctga
    18661 ccgttatttt ggaaaccttg gaaacattgg gctactatgt ttcttggaaa gttttaaatg
    18721 caaaagattt cggcattccc caaaacagga agcgcattta tctgacaggc agtttgaaat
    18781 ccaaacccga cttatctttt gaaacaaccc taagtccgaa attaaaaaat attttggaat
    18841 cgggactgcc taccgaaagc agtcctttca tcaaaaaatt gctaaagaaa tttcccccgt
    18901 ccgaactgta cggaaaatca gtaaaggaca aacggggagg gaaaaacaat atccacagtt
    18961 gggatattga attaaaaggc acagtaaccg aagaagagaa gcaattatta aatatgcttc
    19021 taaaagaacg gaggaagaaa aaatgggctt cagaaatcgg catcgattgg atggatggga
    19081 tgcctttgac aaaagcgcaa atttcaactt tctataaaca ccccaatctt caaaatattt
    19141 tggacagcct gacagataaa ggctatttgg ttttggagca tccgaaacaa aaaattggcg
    19201 ggcgaagaat caaagatgaa tccctgccca aaggctacaa tattgtttca ggaaaaaaat
    19261 catttgaaat caataaaata ttagacccaa atgatgttgc gccaacctta gttgcaatgg
    19321 atatggagca ccttttcgtc gttgacaacg gcggtttgcg cacacttacc ggaaaagaag
    19381 gtttacgctt attcggctat ccggacgatt attcgtttga tattcctaaa aaagacaaat
    19441 acgatttatt gggcaatacc gttgccgttc ctgtgattaa ggcggtatct gaaaggcttc
    19501 tgcatacttt ataaaccgat agggataaaa tggtaaaact tactgcacaa caaatatttg
    19561 ataagttgct gaatgaggaa aaaatcttat cggtcaatgg tcaaattaga tttttcttgg
    19621 gcgatgtgga tattatcgtc aaacaaaaag atgtcgtcgg caacatcatt caagaatggc
    19681 tcggcggatg gttgagaaaa agggaaattg aatttgatgt ttcaaccaat acccaaatgc
    19741 cccccgattt ctttttaaat aaaaaagacc gcagtagaga attgttggaa gtaaaggcgt
    19801 tcaaccgaaa tgccggcccg ggttttgata ttgcagattt taaaatgtat tctgatgaaa
    19861 tcattcataa accctatatg ctggatgtag attatttaat attcggttac gatatggacg
    19921 acaacggcaa tgtaaccatc aaggatttat ggcttaaaaa agtatggcaa attacgagaa
    19981 gcatggatgg gtgggcaatt aatcttcaag tcaaaaaagg cgtggtgcat aaaatccgcc
    20041 cgggtgtttg gtacagcata aataaaaaga atatgcctat gtttgaatgc ttggaagatt
    20101 ttgtttccgc aattgaagaa accgtttatc aaaacccggc aacacggcat aacgcatcct
    20161 tatggaaaaa gaaatttgag gaagcctata aaaaacacta caaccgatcg atttccatac
    20221 ctcgttggca cgaaattgca cacaaataca aaaagaaata acccttcaac catcaaccca
    20281 taaggaaact tgaaatgacc aaacatatcg ccatcctccg aggtgacggc atcggtcccg
    20341 aaatcgtcgc cgaaaccgtc cgcgtactcg acaaactcat cgcccaaggc ttggacatca
    20401 gctacgaata cgccccattg ggtggtgaag cctacgacga atacggccat ccttatcccg
    20461 aatttacgca aaacctctgc cgcaaagccg atgccgtcct gcttggtgca gtcggttctc
    20521 ctcaatacga caacctcgac cgtccgttgc gccctgagcg cggcctgttg gcaatccgta
    20581 aggatttgaa cctgtttgcc aatttgcgtc ctgctgtttt atatcccgaa ttagccaacg
    20641 cctccacgct gaagcctgaa atcgttgccg gtttggatat tttgattgtg cgcgaactca
    20701 ccggcgacat ctatttcggc gaaccgcgcg gcatccgcgt tctggaaaac ggcgaacgcg
    20761 aaggctacaa caccatgaaa tacagcgaaa gcgaaatccg ccgtattgcc catgttgcct
    20821 tccaatccgc ccaaaaacgc agcaaaaaag tctgttccgt aggcaaagcc aacgttttgg
    20881 aaaccaccga actgtggcgc gaaatctttg aagaaatcgg caaacaatat cccgatgtcg
    20941 aactctccca tatgtatgtg gacaacgccg ctatgcagct cgtgcgcgcg cccaaacaat
    21001 ttgacgtgat tgccaccggc aacatcttcg gcgatatttt atcggacgag gcctctatgc
    21061 tgaccggctc catcggtatg ctgccttctg cttctttgga cgaaaacggc aaaggcctgt
    21121 acgaaccgtc tcacggctcc gctcccgaca tcgccggaca aaacaaagcc aacccgctgg
    21181 ccaccatcct ctcgctcgca atgttgctgc gttacagcct gaacgacgaa gcgcgcgcgc
    21241 aacaagttga aaacgccgtc caaaaagtgc tgcaacaagg cttgcgtacc agcgacattt
    21301 acgaagaagg cacaaaactc gtttcctgct ccgaaatggg cgatgcgata ctcgccgcct
    21361 tgtaaaaaaa caaagcgcgg atcacaccgc gctttttcat ctgccctttt ccgcccgcac
    21421 cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg aaatgccata aatccccgcc
    21481 tatgttagga taaacacctt tatcggccca cacaaaggat tatcatcatg aaaaaaatca
    21541 tcttcgccgc actcgcagcg gcagccgttg gcactgcctc cgccgccacc tacaaagtgg
    21601 acgaatatca cgccaacgcc cgtttcgcca tcgaccattt caacaccagc accaacgtcg
    21661 gcggttttta cggtctgacc ggttccgtcg agttcgacca agcaaaacgc gacggtaaaa
    21721 tcgacatcac catccccgtt gccaacctgc aaagcggttc gcaacacttt accgaccacc
    21781 tgaaatcagc cgacatcttc gatgccgccc aatatccgga catccgcttt gtttccacca
    21841 aattcaactt caacggcaaa aaactggttt ccgttgacgg caacctgacc atgcacggca
    21901 aaaccgcccc cgtcaaactc aaagccgaaa aattcaactg ctaccaaagc ccgatggcga
    21961 aaaccgaagt ttgcggcggc gacttcagca ccaccatcga ccgcaccaaa tggggcgtgg
    22021 actacctcgt taacgttggt atgaccaaaa gcgtccgcat cgacatccaa atcgaggcag
    22081 ccaaacaata aatcttgatg cgaatcagca caaatgccgt ctgaacgtcc ttcaaacggc
    22141 atttgtgccg tcttcatcaa accgccccgc caacggtaag ccgatatggc ggagcacaat
    22201 ccgccgaaag atatttcagc ggcaggcagg gcttgccgga aagtccggaa a
//
LOCUS       DAWKOD010000014        21810 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000014 DAWKOD010000000
VERSION     DAWKOD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.014"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(161..532)
                     /locus_tag="WJK73_000400"
     CDS             complement(161..532)
                     /locus_tag="WJK73_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556937.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(666..1109)
                     /locus_tag="WJK73_000401"
     CDS             complement(666..1109)
                     /locus_tag="WJK73_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556938.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1152..2021)
                     /locus_tag="WJK73_000402"
     CDS             complement(1152..2021)
                     /locus_tag="WJK73_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ7556939.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2185..3108
                     /locus_tag="WJK73_000403"
     CDS             2185..3108
                     /locus_tag="WJK73_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7556940.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3186..3827)
                     /locus_tag="WJK73_000404"
     CDS             complement(3186..3827)
                     /locus_tag="WJK73_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7556941.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3882..4673)
                     /locus_tag="WJK73_000405"
     CDS             complement(3882..4673)
                     /locus_tag="WJK73_000405"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ7556942.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4839..4914
                     /locus_tag="WJK73_000406"
     tRNA            4839..4914
                     /locus_tag="WJK73_000406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4872..4874,aa:Thr,seq:tgt)
     gene            4949..5024
                     /locus_tag="WJK73_000407"
     tRNA            4949..5024
                     /locus_tag="WJK73_000407"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4982..4984,aa:Thr,seq:tgt)
     gene            5166..5957
                     /gene="panB"
                     /locus_tag="WJK73_000408"
     CDS             5166..5957
                     /gene="panB"
                     /locus_tag="WJK73_000408"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ7556943.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6080..6916
                     /locus_tag="WJK73_000409"
     CDS             6080..6916
                     /locus_tag="WJK73_000409"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ7556944.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7081..8919
                     /locus_tag="WJK73_000410"
     CDS             7081..8919
                     /locus_tag="WJK73_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556945.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9059..9640
                     /locus_tag="WJK73_000411"
     CDS             9059..9640
                     /locus_tag="WJK73_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ7556946.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9650..10495
                     /gene="ispE"
                     /locus_tag="WJK73_000412"
     CDS             9650..10495
                     /gene="ispE"
                     /locus_tag="WJK73_000412"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ7556947.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            10503..10578
                     /locus_tag="WJK73_000413"
     tRNA            10503..10578
                     /locus_tag="WJK73_000413"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10536..10538,aa:Gln,seq:ttg)
     gene            10587..10662
                     /locus_tag="WJK73_000414"
     tRNA            10587..10662
                     /locus_tag="WJK73_000414"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10620..10622,aa:Gln,seq:ttg)
     gene            10829..11812
                     /locus_tag="WJK73_000415"
     CDS             10829..11812
                     /locus_tag="WJK73_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ7556948.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11879..12451
                     /locus_tag="WJK73_000416"
     CDS             11879..12451
                     /locus_tag="WJK73_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ7556949.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEDQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            13194..14753
                     /locus_tag="WJK73_000417"
     CDS             13194..14753
                     /locus_tag="WJK73_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7556950.1"
                     /translation="MIYIDPPYNTGNDSFVYPDKFSESREEYARRVGDTDDAGYLKRD
                     GVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTLLREDGVIFISIDDNEQAQLKLLC
                     DEVFGAENFVGQFPWRKRTAKSDVPFGVSADYEYLLAYARSNAFIASIDAEERRYFET
                     DDFPNRPWRTHDLTTQRNASERPNSFFTMVNPKTGKEYPANPNATWRVTKDTFQEYYN
                     AGKIVFPDDYDFLKIKNPVMRYFKDDDMKKDGERFGRKAVSTRLPENVGMSLNGTKEI
                     TELFKSKLFDFPKPSSLIKYLLQILHVSDGLILDFFSGSGTTAHAVMQLNAEDGGCRR
                     FICVQLPEETDEKSEARKAGFNTIAEIAKERIRRAGRQISDGLQEGAEIDTGFKVFKL
                     AESGFKQWRQPEQSDTEALQGELSLNIDSVLSEMPSENLLYELMLRLGLKLTCKISLS
                     DGVYFVEDEDTGGLYAFLLERVNQDLIDAVLAKHPVKAVVLDRLFDGDDALKGNTVLQ
                     MKDAGVMFECV"
     gene            14988..17639
                     /locus_tag="WJK73_000418"
     CDS             14988..17639
                     /locus_tag="WJK73_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7556951.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGRNLNAVQKQNGQPETEIGTHGLNFSLEMETGTGKTYVYLRTIYELNR
                     RCGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSTRLSDLRAFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGEAPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKKPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFAEL
                     LEVKQAARGRLKADLNIHYQDKKETKKKKVSVKSGDDLFEKSGGNEAYRHGFIIDGMD
                     FENQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPSGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELSGNTDTAGVHDGYFSQDKKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFGSPLRFIFSHSALKEGWDNPNVFQICMLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWERLKYKTRYRVRFDTEELIKQASAAVAGLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIASRSRDMETTWEIPDILGEIQKKTGLTRRTVYEII
                     IQSGRLKDVPGNPQRFIDLTAEVVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENNFTFAVGNPQKTICENYIPLDSGTEQAFAQNCEDYDAKNEGGVAFYFKLPSWF
                     KIPTPLGNYNPDWAVVKQVQDKVYFVAETKNTGKSIQAGVDAGKLRENERLKIECGER
                     HFEALGGLAYCVVEKVGDLKLEKEEQA"
     gene            complement(17803..18972)
                     /locus_tag="WJK73_000419"
     CDS             complement(17803..18972)
                     /locus_tag="WJK73_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ7556952.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILAITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQIYGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQVLGKIKIRQNGYTIAEKEIVALEDVEKKNWWKKLWARLTGQ"
     gene            19120..20646
                     /gene="ilvA"
                     /locus_tag="WJK73_000420"
     CDS             19120..20646
                     /gene="ilvA"
                     /locus_tag="WJK73_000420"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ7556953.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(20703..21776)
                     /locus_tag="WJK73_000421"
     CDS             complement(20703..21776)
                     /locus_tag="WJK73_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ7556954.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
BASE COUNT         5360 a         5008 c         6031 g         5411 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tttgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agataccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa atagtaagga atatatatat atgcatattc cttactattt tttcacaaat
      661 tatgcttatc tatgcttatt tccataaaaa gcatgccctc gaattttttg acttgagttc
      721 tggaaatgag aataatttga atggatttcc ttctgtccat gtctaataac attttgtctt
      781 ccccaagctt ggtttgctac cgctcttgct tgttgagatg ttcgtgcata gacattagct
      841 cctcttgata aagcttgtgc tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg
      901 cccgcatgca aaattcctat tgccaccaac ggtgccgcag ccccttcagt ttctttcatc
      961 tccttttgag aaagctccgc caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg
     1021 ttttcaaatg tctgattgtc caagccgttt gccattgcgg gggcaatcat agacagcatc
     1081 attacggctg cggtgatttg ttttttcata ataactcctt tggattacaa ggttggaaaa
     1141 tcaaaacctg cttaaaacgt atgctgtacg ccaaatttca gttcggaact gctttgccct
     1201 gaaacgttga aacgtgcgga tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg
     1261 ccgaaatgag cgtaggtgga tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg
     1321 ggctgtgtgc ccagccattg gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat
     1381 gagatgttgg ggttgagcag caggtagttg cccgatttgt agcggatgcc gtctgaaagg
     1441 gttttgctgc cgttgatgcg gtaggcggcg gtgagggaaa ggacaatcgg atctatggct
     1501 ttgtaggtgg tggcgccgat gagccaggat tttcccgacg aggctttgtt gcgcgatttt
     1561 tcgtaaaccg tgctttcaag aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa
     1621 gtgtggctga tgccgaggga tacgtcggac atccgtttgt tgcgggtttt gccgttgccg
     1681 tcgagtttgc gttcttcgtg ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg
     1741 gtcagtccgt agcgcaaacc gagcgtgccg acgagcatat cggtattgct gccgttttct
     1801 tggatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
     1861 gcaagttcgg cgcggttgtt ttcgctgttc aggtaggtaa gggaagtttc cagtttccat
     1921 tttcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agtggataaa
     1981 ggcaggatgg cgggcaaggc gggcaaaaag atgcgcttca tatttctcct attgatatta
     2041 attcttattt actgataata atgaatatcc gataatcttg tctaaataaa tttttaaatc
     2101 ataaaaaaca atatgtttgt ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg
     2161 tgaaaaacgg ttacaatccg ccgcatgaaa aaacgcaata atcctcttcc tctgttgaac
     2221 ggtgtcaaac ccagttattt ggtgctgccg catgaaaagc agttttacgg gctgccgctg
     2281 ctgcattttc tgtgcatccg ctttcctttt gtgggcgcgg acgattggcg caggcggttg
     2341 aacagcggtt ttgtggtcgg ttcggatggt gcgacgttgg acgaacatac tttgttcgag
     2401 ccgggtaagg tgatgtttta ttatcgggaa accagcagtg aggatgagcc gcgtattccg
     2461 tttgaagaaa agattttgca tattgatgag catttgattg tggtggacaa gccgcatttt
     2521 ctgcccgtca tccccagcgg caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg
     2581 cggcctgaat tgcagcattt gaatgttgag gacattacgc cgctgcaccg cttggacaag
     2641 gatacggcag gcgtgatgct gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg
     2701 atgtttcaaa acaaaacggt atggaaaacg tatgaggcgc ttgcgccgac aaggacggat
     2761 ttgccgtatc cgctcgatgt ggtttcgcgt ttggtgaggg gtgagaaatt ttttacgacg
     2821 caagaggcgg aaggcgaacc aaacgcacat acgacggtcg aactgattga aaacaggggg
     2881 gaattcagcc tttaccgcct tacgccgcat acgggcaaga aacaccagtt gcgcgtgcat
     2941 atgatgggtt tgggtatgcc gctgttgaat gacgcgctct atcccgtgcc gtctgaagcg
     3001 ggcagcgagg attatcggaa acctttgaag ctattggcaa aaaagattgc gtttgcagat
     3061 cctttgtcgg gtcgggaaag ggtgttttgc agcggatttt gcctataatg agagggaacg
     3121 caaacccgta tcggcggatg ccgccgatac gggttttaca ttatcgatgc cgtctgaaag
     3181 gggttttatc cgttcaggca tacgggcgcg aagcggagga tggtgtcgcg caggacgatg
     3241 agtttgccgt gaaagaaatg cgtggaaccg gcgatggtaa tgacgggcaa atcttgcggt
     3301 tccgcccatt tcagtgcttt cccgatttcg acgacttcgt cttccgcgcc gtgtatcatc
     3361 agcgttttgg caacattggg aacggcggac ggctcggggc ggccggtata gtggcagact
     3421 gccgcgccga tgagcagcaa taaatcgggt atgcgcgctt gtgcggcaaa tgtggcgaca
     3481 taaccgccga aggagaagcc ggataaggca aattcggggg cttcgggatg ttgggcacgg
     3541 gcatagtcga tgacggcgag gcagtcttgc gtttcgccgc gtccgtaatc gtgtgtgcct
     3601 tcgctgccgc ctacgccgcg aaggttgggt aggtagcagt ggaagccgag tttgcttaag
     3661 gctttggcgg cagtttggat aactttgttg gtgtttgttc cgccctggag ggggttggga
     3721 tgattgatga cggcaacacc gcgtgccggt acttgttcgg acgggatatg gatggtttct
     3781 aaaatgccgg caggaccgga tatgtgtatg gtttcgggtt tcagcataat gatttcctct
     3841 tgattttccg catgttccaa tgcagccgtg aagatagttg cttaaaggtg gaaatgcttg
     3901 ccgttgttgg ggttggacgc tttcaggccg gcaagcatac ggtggaagtc caatccgtat
     3961 gcgttgctca gtttgtcggc acgttttttg agtttgtcta tgttttgctc tttggggctg
     4021 ctttggaagg ccattaccgc gacattgccg tggctttcgg caggaagttc caagacgcgc
     4081 ccttcaaaaa cgctcaacaa ccgttcgatg aagcgttggt agcgtttgtc gccgctccac
     4141 cagttggtta cgaatatgcc gtctgaagag agtgcgtttc ggcagtctcg gaaaaacggt
     4201 tcttcaacca gcgcatcgat gatttgttcg ccgtcgaacc cgtccaccaa aatcacatcg
     4261 gtgttgtggc ggaagacttt gatatattct gcaccgtctg cttcaataat ttcaaatttc
     4321 tcgccctcga aaggcaactc aaacaggttg cgggcaatgg cgatgacctg aggattgatg
     4381 tccacggcgg tttggcgcgt gtcgggcagg taggtgtcta tccagcgtgc aaacgagccg
     4441 ccgcccaagc cgatttgggt gatatgctgc ggaagtgcgt cggtaaacag cagccagccc
     4501 atcatcgcgc ggctgtaaga gagtaccagc tcggacgggt ggtcgaggtt cattgagctt
     4561 tgaacggtgt cgctgcccaa gtgaagcgaa cggatattgc cttcttcgga aatgccgact
     4621 tcgggaaagc cggattttgc aggacgcagg cggcggtagg gatgtcttgc catcggtgtg
     4681 aacggtcggt ttgaaaggcg gatattttat cagaatgccg atgcttgtcc tgcttcaaat
     4741 taatttcttt tcaaataaat tacttattcg gatttgccgg ggctttcgga taaattcctt
     4801 gccaaagtgt gaagttgcct gcataattcg ccttctttgc cggtatagct cagttggtag
     4861 agcacctgac ttgtaatcag ggggtcccga gttcgactct tggtgccggc accatttctg
     4921 ctgatggtgt tgcaaaacat ttcagtaagc cggtatagct cagttggtag agcacctgac
     4981 ttgtaatcag ggggtcccga gttcgactct tggtgccggc accagatttg acagtccgat
     5041 tgtgtaaaac agtcgggctg ttttgcattt gcggcacggt cggtggcagg gctgcgggca
     5101 tttcactata atagcccgtt tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat
     5161 atctgatgat tactgtgaac acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta
     5221 tgctgaccgc ttacgaatcc agttttgccg cgctgatgga cgatgccggc gtggatgtgc
     5281 tgctggttgg ggattctttg ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca
     5341 gcctgcgcga tatgtgttat cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga
     5401 ttgtcagcga tttgccgttt ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg
     5461 ccgccgaact gatggctgcc ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg
     5521 cggaaacgac cgaatttctg caaatgcgcg gtattccggt ctgtgcccac atcggtctga
     5581 ccccgcaatc cgtgtttgcc ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc
     5641 aggcgttgct taacgatgcc aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg
     5701 agtgtgttcc tgcggaactg gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg
     5761 gcatcggcgc gggtgtcgat tgcgacgggc aggttttggt gatgcacgat atgctcggca
     5821 ttttcccggg taagacggtg aaattcgtca aaaactttat gcaggggcaa agcagcattc
     5881 aggcggcggt tcgggcgtat gttgccgaag tcaaagccaa aaccttccct gctgcggaac
     5941 atatttttgc agactgaaca aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg
     6001 cattttgttt tgccgtgcgc ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg
     6061 aaggataagg attaccgtta tgcaaatcat acataccatt caagaactgc gcgcgtggcg
     6121 taaaaatgcg ggaacggtgg catttgtgcc gaccatgggc aatctgcacg aagggcatct
     6181 tgccctcgtt cgcgaagcca aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa
     6241 ccgcctgcaa ttcggtcagg gcgaggattt cgacaaatat ccgcgtactt tgcaacagga
     6301 tgcggacaag ctggaagccg agggtgttgc cgttgttttc gcgcccgatg agaaagaact
     6361 ctatccgaac gtggaacagc gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg
     6421 cggcaagttt cgtccgggac atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa
     6481 tattgtcgcg ccggatgttg cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat
     6541 taaaggtttg accgaggatt tgaattttga tgttgaaata gtgcctgtcg atacggggcg
     6601 cgcggaagac ggtttggcac tctccagccg caaccagtat ttgagtgcgg cggaacgcga
     6661 cgaagcaccg cgcctgtacc gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag
     6721 tttggattat gcaggtttgg aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt
     6781 ggtcgattat gtcgaaatcc gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa
     6841 gaaactggtg gtcttggccg ccgcccgtct ggggacgacg cgcctgattg acaatttgga
     6901 aataaaactc ccttaaaccg caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca
     6961 gacggcattt atttttttga acggggtttc gcaaatctac aaacgattct gcttgtgata
     7021 aagttacgcc tgattatatg ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt
     7081 atgttacccg cccgtttcac cattttatct gtgctcgcgg cagccctgct tgccgggcag
     7141 gcgtatgccg ccggcgcggc ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga
     7201 aagcagcagc gttacagcga ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg
     7261 ggcgagcggg ttaatcagat atttacgttg ctgggagggg aaaccgcctt gcaaaagggg
     7321 caggcgggaa cggctctggc aacctatatg ctgatgttgg aacgcacaaa atcccccgaa
     7381 gtcgccgaac gcgccttgga aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg
     7441 atttatcaga aatggcggca gattgagcct ataccgggtg aggcgcaaaa acgggcgggg
     7501 tggctgcgga acgtgctgag ggaaagagga aatcagcatc tggacggact ggaagaagtg
     7561 ctggctcagg cggacgaagg acagaaccgc agggtgtttt tattgttggc acaagccgcc
     7621 gtgcaacagg acgggttggc gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa
     7681 tatgaacatc tgcccgaagc ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa
     7741 aaggaaaagg caatcggagc tttgcagcgt ttggcgaagc tcgatacgga aatattgccc
     7801 cccactttaa tgacgttgcg tctgactgca cgcaaatatc ccgaaatact cgacggcttt
     7861 ttcgagcaga cagacaccca aaacctttcg gccgtctggc aggaaatgga aattatgaat
     7921 ctggtttccc tgcacaggtt ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc
     7981 aatccgaatg cagacctgta tattcaggca gcgatattgg cggcaaaccg aaaagaaggt
     8041 gcttccgtta tcgacggcta cgccgaaaag gcatacggca gggggacggg ggaacagcgg
     8101 ggcagggcgg caatgacggc ggcgatgata tatgccgaca gaagggatta caccaaagtc
     8161 aggcagtggc tgaaaaaagt gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg
     8221 gctgcggcgg ctgtcgagtt ggacggcggc agggcggctt tgcggcagat cggcagggtg
     8281 cggaaacttc ccgaacagca ggggcggtat tttacggcag acaatttgtc caaaatacag
     8341 atgttcgccc tgtcgaagct gcccgataaa cgggaggctt tgagggggtt ggacaagatt
     8401 atcgaaaaac cgcctgccgg cagtaataca gagttacagg cagaggcatt ggtacagcgg
     8461 tcagttgttt acgatcggct tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg
     8521 ttcaggcttg cacccgataa cgctcagatt atgaataatc tgggctacag cctgctttcc
     8581 gattccaaac gtttggacga aggcttcgcc ctgcttcaga cggcatacca aatcaacccg
     8641 gacgataccg ctgtcaacga cagcataggc tgggcgtatt acctgaaagg cgacgcggaa
     8701 agcgcgctgc cgtatctgcg gtattcgttt gaaaacgacc ccgagcccga agttgccgcc
     8761 catttgggcg aagtgttgtg ggcattgggc gaacgcgatc aggcggttga cgtatggacg
     8821 caggcggcac accttacggg agacaagaaa atatggcggg aaacgctcaa acgtcacggc
     8881 atcgcattgc cccaaccttc ccgcaaaccc cggaaataat gcaggtccat cctttcagac
     8941 ggcataaggt ttgccgggaa gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt
     9001 tttgcacggc acgccgaacc cgccggccgg caggatggca cccgttaagg aaattctgat
     9061 gaaacacacc gtatccgcat cggtcatcct gcttttgacc gcttgcgcgc aattacctca
     9121 aaataacgaa aacctgtggc agccgtccga acacatcagc agttttgcag cagaagggcg
     9181 gttggcagtg aaagcggaag ggaaaggttc gtatgcaaat ttcgattgga cataccaacc
     9241 gcccgtggaa accatcaata tcaatacccc tttgggcagt acgctcgggc agttgtgtca
     9301 agacagggac ggcgcattgg cagtggacgg caaaggaaat gtctatcagg cggaaagtgc
     9361 ggaagaattg agcaggcagc tggtcggttt caaactgcca atccaatatc tgcatatctg
     9421 ggcagatggc aggcgtgtgg cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga
     9481 acaatacggt tggactgtcg gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca
     9541 actgaataac ggaaatttga acatcaggct ggttttcacc gaaatcggta tgccgtctga
     9601 aactgaaacc ccggaacgct gtgcggcgcg cacgagataa ggcggacaga tgaatattgc
     9661 ggacggacgg caggcgtttc ccgcacctgc aaaactgaat ctcgatttga ggattaccgg
     9721 caggcgggaa gacggttatc acaatatcga aagcatattc tgcctgatag atttgcagga
     9781 taccgtatat ttgaaatcga gggatgacgg caaaatcatc ctgcacaatc cggttgatgg
     9841 aatgccgcag gaagcggatt taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg
     9901 caaccccgcc ggcgtggaaa tatggctgga caaaaaaatc ccgacagggg cgggtttggg
     9961 cggcggaagc tcggatgcgg caaccgtttt gctggtgttg aaccgttggt ggcagtgcgg
    10021 tctgacgcag cggcagctca ttgattcggg cgcggctcta ggggcggacg taccattttt
    10081 tattttcgga aaaaatgctt ttgcaagcgg gataggtgaa aaactgatag gaatggatat
    10141 tccgaaacag tggtatgtca tcgtcaaacc gcccgtccac gtttccactg caaaaatttt
    10201 cacacacgaa ggcttgacac gaaattccgc ctcaagcata atgccgactt tccaaaacct
    10261 gcaaccgttt agaaatgaca tgcaggcagt tgtatttaaa gaataccctg aagtttggaa
    10321 agcctattcc gaattgtccc gatatggatt tgccttaatg acaggttctg gtgcgtgtgt
    10381 attcacggcg tgtcaagata ggaatagcgc atacaatata taccgacaag tttcagattt
    10441 gtacgaggca tatttggcag agggtctttc aaaacatcct ttgttgtccg tataaacatt
    10501 gttggggagt cgtcaagcgg ttaagacact ggattttgat tccagcatgc gaaggttcga
    10561 atccttcctc cccagccagc cctttttggg gagtcgtcaa gcggttaaga cactggattt
    10621 tgattccagc atgcgaaggt tcgaatcctt cctccccagc caaataaaag cgtgtaagcc
    10681 tgcttacacg cttttatttc atagaaataa aaatattgaa atgcctttgt ttgtgtcgga
    10741 tgttgcaggt ataatgtcgg gcttggtaca agcagaggga agcattgtgt tttctgagcg
    10801 gaagttaaac ataaaatcag gtgagaatat ggctgcgtac gacagtttga tggtatttac
    10861 aggcaatgcc aatcccgaat tggcacaacg tgttgtcagg catttggaca tttctttggg
    10921 caatgcttcc gtatccaagt tttcagacgg cgaagttgcc gtcgaactgt tggaaaacgt
    10981 acgcgggcgc gatgttttca tccttcagcc gacctgtgcg ccgaccaatg acaacctgat
    11041 ggaaatcctg acgatggcgg atgcactgaa gcgtgcttcg gcaggtcgta ttaccacagc
    11101 cattccgtat ttcggctatg cgcgccaaga ccgccgtccg cgttccgtcc gcgttccgat
    11161 ttctgccaaa ctggtggcaa atatgctgta ttcggcaggg atcgaccgtg ttttgactgt
    11221 cgatttgcat gccgaccaga ttcaaggttt cttcgatatt ccggtggaca atatttatgc
    11281 caccccgatt ctgttgaacg acatcaaaca acagcggatt gaaaatctga ccgtcgtcag
    11341 cccggacatc ggcggtgtcg tccgcgcccg cgccgtggca aaatccctga atgccgactt
    11401 ggcaatcatc gacaaacgcc gcccgaaagc caatgtggcg gaagtcatga acatcatcgg
    11461 cgatattcaa ggcagaacct gtctgattgt ggacgatatg attgacaccg caaatacgct
    11521 gtgcaaagcc gccgtcgctc tgaaagagcg gggggcggaa cgtgtcttgg catatgccag
    11581 ccacgccgta ttctccggag aggcagtcag ccgtattgcc tcatccgaaa tcgaccaggt
    11641 ggtcgtaacc gataccattc ctttgtctga agcggctaaa aactgcgacc gtatccgtca
    11701 ggtaacgatt gccggtctgt tggccgaaac cgtccgccgc attagcaatg aagaatccgt
    11761 ctcatatctt ttcaatgaag aagtgatgac aggcagcatg ttgctgccat aagcccgaag
    11821 ccgtcttaag ctggtcgcgg ccgatgacgg cgattttacc taacttggag tatttaacat
    11881 gacttatgaa attcaagctt ctgttcgtga agcacaaggc actggtgcga gccgccgcct
    11941 gcgtcgcgaa ggccaaatcc ccggcattct gtacggtgaa gaccaagagc ctgttgcaat
    12001 cgctgtggat cacaaaaccg tattctacgc attggaaaaa gaatctttcc atactgcgtt
    12061 gattaagttg tctctgaacg gtgaaaccaa agacgttatc gtccgcgatt tccaaatgca
    12121 cccgttccgc cgcgaagttc aacacatcga cttccaagct gtgaaagccg atcaacttgt
    12181 acgcatccgt gttcccctgc acatcgttaa cgctgaaaat tcccaagcgg tcaaactgca
    12241 aggcggccgc gtatctctgt taaacacttc tgttgaagta gttgctttgc ctgccaacat
    12301 ccctgctttc ttggatttgg attgtgccga agtggttgcc ggcgacattc tgcacttgtc
    12361 agacatcaaa ctgcctgaag gtgtagaaag cgtttccctg aaacgtaacg aaaatctggc
    12421 tgttgctacc gttaccggta agaaacgcta attgattgaa aagcaggcgg tacgtatatg
    12481 atgcgtaccg ccttattgtt tttggctgtg tttcaatatt tggttgattt catccgttta
    12541 taagggaaaa ccgcttattt ccgttcgggt ggaaatttat ccagacgtat tgcgcgacaa
    12601 cggatggttg gctcaaaaac ggcatttcgt accggatgaa aatgaagaag atatgtcgtt
    12661 cacattccgg tagctgtccg gctcgaaccg gctatcagag atagtcggtc aattttcacc
    12721 ctgaaaatca cttttctttt aacttgcaac atattccgat aaaatggaca ctttgtaagt
    12781 aatcattgat ggaagacgca attatggcaa ccgaaccgaa ccgaaccgaa ccgaaccgaa
    12841 ccgaaccgaa ccgaaccgaa attatacacg atttagaaga taatttcaaa caaaacaata
    12901 tattaaaact caaacaactc tttcccgaag tcttttgtga agaccaaatt gactttgaaa
    12961 agctcaaact cgtcctcggc gcagaaaacc ttgccggtgc gggcgaacgt tatcaactcg
    13021 attgggcggg caaaaccgcc tcctatctca acctgcaaag ccccacttcc cgcaccctcg
    13081 tcccctgcaa agaagaatcc gcagatttcg acggcacgca aaacgtgttt atcgaagccg
    13141 aaaacctcga agcactgaaa atcctgcaaa aatcctacgc cggcagcgta aagatgattt
    13201 acatcgaccc gccctacaat accggcaacg attccttcgt ctatcccgac aaattctccg
    13261 aaagccgcga agaatacgcc cgccgcgtgg gcgataccga cgacgcgggt tacctgaagc
    13321 gcgacggcgt gttccagggc gcgtggcgca aaaacggcaa agacagtggc cattaccaca
    13381 gcaactggct ctctatgatg ctgccgcgcc tgcatctggc gaaaaccctg ctgcgcgaag
    13441 acggcgtgat ttttatcagc attgacgaca acgaacaggc gcagttgaaa ttgttatgtg
    13501 atgaagtgtt tggggcggag aattttgtcg gtcagtttcc ttggcgcaaa cgcacggcaa
    13561 aatcggacgt gccgttcggc gtttccgccg attatgaata tctgcttgcc tatgcccgca
    13621 gtaatgcctt tattgccagt atcgatgcgg aagaacgcag atattttgaa accgatgatt
    13681 tcccgaatcg cccgtggcgg acgcatgatt tgactactca aagaaatgcc agtgaacgac
    13741 caaatagttt tttcactatg gttaatccta aaacaggcaa agaatatccg gcgaatccaa
    13801 atgcaacttg gcgtgttact aaggatacat ttcaagaata ttacaatgcg ggaaaaatcg
    13861 ttttccccga tgattacgat ttcctcaaaa ttaaaaaccc cgttatgcgt tattttaaag
    13921 atgacgacat gaagaaagac ggagagcgct tcggacgtaa ggcagtcagt acacggctac
    13981 ctgaaaatgt aggtatgtcg ctgaacggta caaaagaaat taccgaactg tttaaaagca
    14041 agttgtttga ttttcccaaa ccgtcatccc taattaaata tttactgcaa atcctccatg
    14101 tttcagacgg cctcatccta gacttcttct ccggcagcgg cactaccgcc catgccgtga
    14161 tgcagcttaa cgccgaagac ggcggctgcc gccgtttcat ctgtgtccag cttcccgaag
    14221 aaaccgacga aaaatccgaa gcgcgcaagg cgggtttcaa cacgattgcc gaaattgcca
    14281 aagaacgcat ccgccgcgcg ggcaggcaga tttcagacgg ccttcaagag ggagcggaaa
    14341 tagatacggg ttttaaagtt ttcaaacttg ctgaaagcgg tttcaaacaa tggcggcagc
    14401 ccgagcagtc cgatacggaa gccctgcaag gcgagttgtc cttgaacatt gattccgtgt
    14461 tgtctgaaat gccgtctgaa aacctgctct acgaattgat gttgcgttta ggtttgaagc
    14521 tgacttgcaa gatttcgctt tcagacggcg tgtatttcgt ggaagacgag gatacgggcg
    14581 gactgtacgc cttcctgctg gaacgcgtga atcaggattt gattgatgcc gtgctggcga
    14641 agcatcccgt caaggcggtg gttttggaca ggctgttcga cggcgacgac gcgctcaaag
    14701 gcaataccgt attgcagatg aaggacgcgg gcgtgatgtt cgaatgcgtg tagtcagccg
    14761 aaagcgggaa gtgccgtctg ccgtcattcc cgcgcaggcg ggaatctaga aactcaaagt
    14821 tgcaagaatt tatcggaaat gactgaaacc caacgaacct agattcccac tttcgtggga
    14881 atgacgatat atcagtttct gcgccgacag gtctggattc ccgcctgcgc gggaatgacg
    14941 gcaaagaggt agattttccg cgcgacagcg gatagaagga aataaaaatg gaactgaaat
    15001 ttgaagaact gccctatcag cttgatgcgg taaatgccgt tgcgaatctt tttgcagggc
    15061 agccgaacca cgcccggaca tttgatttga ccagtcaggg gacggggcgg tttgtcggca
    15121 acggcttgga tttggattgg gaaacgctgg gtcggaatct gaatgcggtg caaaagcaaa
    15181 acggacagcc tgaaacggaa atcggcacac acgggctgaa tttttctctg gaaatggaaa
    15241 cgggtacggg caaaacctat gtctatctgc gcacgattta cgaattgaac cgccgttgcg
    15301 gctggaagaa atttgtgatt gtcgtgccga gtgtgccgat acgcgaaggc gtgttgaaaa
    15361 gcctggaaat tacgaaggcg cattttgacg gagtgttcgg caggcccgtg atgcgttatg
    15421 cggaatattc aagcacgcgg ctgtcggatt tacgcgcttt tgcggtaaac gaccatatcg
    15481 aaattttggt aatcaatatc cagtcgtttg aaaaagacgg aaacgtgatc aataaggtaa
    15541 acgaaagcgg ggaagcaccg attaagttta ttcaggaaac gtgtcctatc gtcattatcg
    15601 acgagccgca gaatatggaa acggaaggcc gtctgaatgc cttggaatcg ctcaatccgc
    15661 tctttaccct gcgctattcg gcaacgcata aaaaaccgta tcacaaggtg tacagcctga
    15721 atcccgtgca ggcatacaat ttgaagctgg tcaagcaaat cgaagttttg ccggtattgg
    15781 cggaaaacga tgtgaacggc gcatttgcgg agctgctgga agtgaaacag gcggccagag
    15841 gccgtctgaa agccgatttg aatattcatt atcaggataa aaaggaaacc aagaagaaaa
    15901 aggtatccgt caaatcggga gacgatttgt ttgaaaaatc gggcggcaac gaagcctacc
    15961 gccacggttt cattatcgac ggcatggatt tcgagaatca ggaattggcg ttctccagcg
    16021 gtaaaaccat tacgcgcggc ggagacggcg atgcggtgcg cgatgaaatt atgaaaaacc
    16081 aaatccgcga aacggtaagg gagcatttgg cgaaggagaa acggctgaat ccgtcgggga
    16141 ttaaggtgtt gtcgctgttc ttcattgacc gcgtggagaa ttaccgcaca gaagacggaa
    16201 cggcaggcaa gtttgccctt tggtttgaag aaatttatcg ggagctttca ggcaataccg
    16261 atacggcagg cgtacacgac ggctatttct ctcaagacaa gaaaggccgt ctgaaaaaca
    16321 ccgacggcac gacgcaggcg gacaacgata cctaccgcct cattatgcgc gacaaggaaa
    16381 ccctgctttc cttcggcagc ccgctgcgct ttatcttttc ccattccgcg ctgaaagaag
    16441 gctgggacaa ccccaacgtt ttccaaatct gcatgctcaa cgaaacccgc tcgccgttga
    16501 aaaagcggca ggaaatcggg cgcgggctgc gtatcgccgt caatcaggaa ggacggcgcg
    16561 tgcgcgatga aaacgtgaac atactgaccg ttgtgccgaa cgaaagctac gaattgtttg
    16621 ccgcaaatct gcaaaaggaa tacgaagacg aatgcggtat caaatttgcc gccgaaaaca
    16681 tcaaaaacgg caataagaag aaaaaacagc tattccgcaa agattttccg ctcgatcctg
    16741 agtttcaggc aatttgggag aggttgaaat acaaaacgcg ctaccgtgtg cgcttcgata
    16801 cggaagaatt gattaaacag gcttcggcgg cagtagcagg cctgcccgaa atccagaagc
    16861 cgaaaatccg catccgtaag gcgcaattga aacaatctca aacctacggt atcgaaacag
    16921 tcgaaattgc cagccgcagc cgcgatatgg aaaccacatg ggagattccc gacatactcg
    16981 gcgagatcca aaaaaagacg gggctgacgc ggcgcacggt gtacgaaatc atcatacagt
    17041 caggccgtct gaaagatgtg ccgggcaacc cgcaacgctt catcgacctt accgccgaag
    17101 tggtaaaccg cagcctgcac gcgttgatgg cggcaggaat cgagtatgag cgaaccggcg
    17161 aagtgtacga ccaaaagctg ttggaagata tggcgcgcga aggcatcgag ttttatgaaa
    17221 acaactttac gtttgcagtc ggtaatccgc aaaaaaccat ttgcgaaaac tatatccctc
    17281 tggattccgg cacggaacaa gcctttgccc aaaattgcga ggattatgat gcgaaaaatg
    17341 aaggcggcgt ggcgttttac ttcaaactgc ccagctggtt caaaatccca accccgctgg
    17401 gcaactacaa tcccgattgg gcggtggtga aacaggttca ggacaaagtg tatttcgttg
    17461 cagaaaccaa aaataccggc aaaagcattc aggcgggggt agatgcgggc aagctgcgcg
    17521 aaaacgaacg gctgaaaatc gaatgcggtg aacggcattt tgaagcactg ggcggtttgg
    17581 catactgtgt tgtggaaaaa gtgggggact taaagttaga aaaagaggag caggcttaaa
    17641 acgaacaggt gtaatcatag tatggtgatg tagtgcttgg ggcgggcggc aactgaagat
    17701 gcggatgcgg caattacctt gattcactaa accgattggg ggttgaagat tgtttcagac
    17761 ggcattttaa ttctcctatc ttgggcatgg aaatatagaa gcttactgtc ccgtcaggcg
    17821 tgcccagagt tttttccacc agtttttctt ttctacgtcc tccagtgcga caatttcttt
    17881 ttcggcaatg gtgtatccgt tttgtctgat tttgattttt cctaggacct gccctttttt
    17941 taccggggcg ggaatcggct gtatggtttc caaaatctgt tctgccattt tcgcttcctt
    18001 atgtggcaga gtgatgtagg cttctttgag gaagcctgcg cggacggttt ttttgctgcc
    18061 tccgtaaatt tggatttggg caacggtttt tcctttcgga tatattttgg gcgtatcgaa
    18121 ggcctgcaat gcccagttca gcagcttgct gttgtccgat gcgcgtgttt ccgccgattc
    18181 cgaacccaat gtgatggcaa ggatgtgcct gccgttgccg gagtatgaca cggcaaggtt
    18241 gtagccgccg ctttctgtgt gtccggcttt cagaccgttt acattgttgt ccctatataa
    18301 aaggatattg cggttgtttt gttctatatt tttgaatttg aaagatttga tggaaaacag
    18361 cgggtaatat tccggaaagt cgcgcatcaa tgcttcagac agcagggcga ggtctttggc
    18421 ggtggaaacc tgtccttctc tactcaagcc tgtcgggttt ttgaatacag tgttcttcat
    18481 gcccaagcgt cgggcttctt tgttcatttg ttgcacaaaa ttttcaatcg agccgttgcc
    18541 cagccggccg gcaagggtta gggcggcatc gtttgcggat agtgcaatca tgccttttaa
    18601 gagtttgtcg gtgctgaccg tatcgccggg acgtacaaac attctgcttc cttctgaagc
    18661 ccatgcggat tcgggtattt ttaagttttc ttcagatcgg atatttcccg atttcatgtt
    18721 tttgaaaacc agatatgcgg tcatcagttg ggttagtgcc gccggttcga caggggtatt
    18781 gatgtttttg gcggataaaa tctgtttgct ttgaaggtcg ataacgatgt gtgccgctgt
    18841 gagggtttcg ggtgtttgga acgtgggggc ggcgtgtacc gccggtttgt tgggcgcggg
    18901 cgatgcggcc gttgcgtgag aaacgcctaa gatgatggaa agcaggacgg gcaggatttt
    18961 atgtgctgtc atgaaatatt ctaattgtgt gcgtgtttca gtctgccgat tatacgctta
    19021 gggtgtctga tcgggcggat ttttcttgct ttcgcgccgt cttgggcgta tggttttggg
    19081 ttttgcgatt ttaataaacc gattatccca tattgaatta tgaacacgcc ccttccttat
    19141 tccaattacc tcatccgcat cctgacggca tctgtctatg atgtcgcggt cgaaacgcct
    19201 ttggaaccgg cacgcagcct ttctgtacgt ttgaaaaaca acatcctttt gaaacgcgaa
    19261 gatttgcagc cggttttttc gttcaaaata cgcggcgcgt acaacaaaat gtccaagttg
    19321 ccgaaagatg cgctcgcttg cggcgtgatt gcggcaagcg cgggcaatca cgcacaaggc
    19381 gtggcattgt ccgcacagcg tttgggttgc cgtgccgtta tcgtcatgcc ggagactacg
    19441 ccgaaaatca aagtggatgc ggtcaaaagc cgtggcggcg aggtggtttt gcgcggtgtt
    19501 tcatacaacg atgcttacga ttatgcgatg gagctggcgg aacaggaagg gctgacctat
    19561 atcgcaccgt ttgacgatcc tgatgtgatt gcaggacaag ggacggtagg gatggaaatt
    19621 gtcagccagc atcccgatcc aatccgcgcc gtattcgtac cgataggcgg tggcggtttg
    19681 gcggcgggcg tggcggcatt tatcaagcag gtccgtcccg aaatcaaagt tatcggcgtt
    19741 cagaccaacg attcctgctg tatgaagcag tcggtcgaag cgggtgaaat cgtccatttg
    19801 aaagatgtcg ggctgttttc agacggcacg gcggtcaaag tcgtcggaaa cgaaaccttc
    19861 cgcctctgca aagaactttt ggatgaaatc attacagtcg ataccgatgc ggtttgcggc
    19921 gcggtcaagg atattttcga tgacacgcgc agcattaccg agccggcggg cgcgttggcg
    19981 ttggcgggtc tgaaagccta tatcgcccga gaaggcgcgg aaaaccaaac cctgattgcc
    20041 gttaccagcg gtgcgaatat gaattttcac cgtttgcgcc acgtttcgga acggagcgaa
    20101 ttgggcgagg gcaacgaagg tatttttgcc gttaccatcc ctgaagaacg cggcagcttc
    20161 cttaagtttg tcaatatatt gggaaatagg aatattaccg agttcaacta ccgctacggg
    20221 gacgatgaaa aagcgcatat ctttgtcgga cttcaagcgg caggcccgca ggatttggcg
    20281 gttatcggca gccggttgga tgaggcggga ttgcccaatg tcgacctgac cgatgatgag
    20341 attgccaaaa tccatatccg ctatatggtc ggagggcgga cggacaaagt agaaaacgag
    20401 cgtctggtca gttttgagtt tccggagcgt ccgggggcgt tggcgcgttt tttgaaccat
    20461 atgcagggcg gttggaacat tacgctcttc cattaccgca accacggtgc ggattacggg
    20521 cggattttgg tcggtatcga tgtgccgccg cacgatgccg ccgcatttga cggtttcttg
    20581 gaaagtctgg gatacagcta tcacgaggaa acgcaaaatg ccgcgtacaa gctgtttctt
    20641 gcctgacgct tgaaagcaca atgccgtctg aaagcctttc agacggcatt gcgctttcat
    20701 ggttaaatcg aatattcaat cagttcgttt tgagagaaga cataaacctg tttcggaatc
    20761 agcttcaatt ctttgccttc ggcgattggg taacgcgcgg catcgctgcc agccagcgtg
    20821 atatgcacgt cttgtttgcc gtgtttcacc aaaatatgcg tcaatgcccc gacggcgtgg
    20881 actttttcga tttcggcaca aatcatcggt gtttcgtgtt cggcggcgat ctgccattcg
    20941 tgcgggcgga tatagccggt ggcggtttgt tcctgccatt tgtattgtgc gtccaatttc
    21001 cacgcgaagc cgttataatg ccagaagcct ttttcgatgc gtccttcaaa agcgtcggtt
    21061 tcgccgagga actcggtaac gaaggcattt tcgggtttgc ggtaaatagc ttcggcgctg
    21121 ccggtttgtt cgattttgcc gtggttcatc acgacgattt cgtcggaaac ttcgagggct
    21181 tcttcttggt cgtgcgtaac cagaatgctg gttacaccca ggttgtgatg gatgtcgcgc
    21241 agccaggtgc gtaattcttt gcgtactttg gcatccaacg cgccgaaggg ttcgtccaaa
    21301 agcaggagtt tcggttcgac cgcaagcgca cgggcgaggg cgatgcgctg gcgttgcccg
    21361 ccggagagtt ggtgcggata ggattttgcc aaatgcgaga gctgcacgag cttgagcaat
    21421 tcttcgactt tggcgcggat ttgtcctttg gacgggcgtt cggacttggg caatacggtc
    21481 aaaccgaagg caacgttgtc aaacacgttc atatggcgga aaagggcgta gtgttggaac
    21541 acgaagccga ctttgcgctc gcgcacgtgc ttggcggtta cgtcttgccc atcaaacaga
    21601 atcttgccgc cgtcggcgtt ttccagtccg gcgataatgc gtaaaagcgt ggttttgccg
    21661 cagccggacg ggccgagcag ggaaacgagt ttgccggtgg ggacgttgag gttgatgttt
    21721 ttcagcgcgt gaaaattgcc gaagtgtttg tttaagtttt ggatggtgat actcatattg
    21781 cattcctttc ggcggcggcg agttttttgt
//
LOCUS       DAWKOD010000015        21585 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000015 DAWKOD010000000
VERSION     DAWKOD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.015"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..2021)
                     /gene="uvrC"
                     /locus_tag="WJK73_000422"
     CDS             complement(135..2021)
                     /gene="uvrC"
                     /locus_tag="WJK73_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ7556955.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(2076..4253)
                     /locus_tag="WJK73_000423"
     CDS             complement(2076..4253)
                     /locus_tag="WJK73_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ7556956.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4392..5342
                     /gene="trxB"
                     /locus_tag="WJK73_000424"
     CDS             4392..5342
                     /gene="trxB"
                     /locus_tag="WJK73_000424"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ7556957.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5498..5866
                     /gene="rpsF"
                     /locus_tag="WJK73_000425"
     CDS             5498..5866
                     /gene="rpsF"
                     /locus_tag="WJK73_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ7556958.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5867..6169
                     /gene="priB"
                     /locus_tag="WJK73_000426"
     CDS             5867..6169
                     /gene="priB"
                     /locus_tag="WJK73_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ7556959.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6176..6406
                     /locus_tag="WJK73_000427"
     CDS             6176..6406
                     /locus_tag="WJK73_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ7556960.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6423..6875
                     /locus_tag="WJK73_000428"
     CDS             6423..6875
                     /locus_tag="WJK73_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ7556961.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6889..6996
                     /locus_tag="WJK73_000429"
     CDS             6889..6996
                     /locus_tag="WJK73_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ7556962.1"
                     /translation="MPSETLPIVGMWFQTVFVLFFVYSAKIGMVVFIVD"
     gene            complement(7663..8469)
                     /locus_tag="WJK73_000430"
     CDS             complement(7663..8469)
                     /locus_tag="WJK73_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7556963.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8470..9216)
                     /gene="pssA"
                     /locus_tag="WJK73_000431"
     CDS             complement(8470..9216)
                     /gene="pssA"
                     /locus_tag="WJK73_000431"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ7556964.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            9799..10282
                     /locus_tag="WJK73_000432"
                     /pseudo
     CDS             9799..10282
                     /locus_tag="WJK73_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(10334..11545)
                     /locus_tag="WJK73_000433"
     CDS             complement(10334..11545)
                     /locus_tag="WJK73_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ7556965.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11768..14206
                     /locus_tag="WJK73_000434"
     CDS             11768..14206
                     /locus_tag="WJK73_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ7556966.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            14414..15724
                     /locus_tag="WJK73_000435"
     CDS             14414..15724
                     /locus_tag="WJK73_000435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ7556967.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15820..16434
                     /gene="clpP"
                     /locus_tag="WJK73_000436"
     CDS             15820..16434
                     /gene="clpP"
                     /locus_tag="WJK73_000436"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ7556968.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(16487..17752)
                     /gene="ispG"
                     /locus_tag="WJK73_000437"
                     /gene_synonym="gcpE"
     CDS             complement(16487..17752)
                     /gene="ispG"
                     /locus_tag="WJK73_000437"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ7556969.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17768..18529)
                     /gene="pilW"
                     /locus_tag="WJK73_000438"
     CDS             complement(17768..18529)
                     /gene="pilW"
                     /locus_tag="WJK73_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ7556970.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(18532..19626)
                     /gene="rlmN"
                     /locus_tag="WJK73_000439"
     CDS             complement(18532..19626)
                     /gene="rlmN"
                     /locus_tag="WJK73_000439"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ7556971.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19770..20195)
                     /gene="ndk"
                     /locus_tag="WJK73_000440"
     CDS             complement(19770..20195)
                     /gene="ndk"
                     /locus_tag="WJK73_000440"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ7556972.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(20254..21405)
                     /locus_tag="WJK73_000441"
     CDS             complement(20254..21405)
                     /locus_tag="WJK73_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ7556973.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5192 a         5328 c         5872 g         5193 t
ORIGIN      
        1 agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttaaat
       61 ttaatccact atatatcggt ataccgacct gctgtatcat attttttaca gacggttttt
      121 gcttgggcag catactaatg cagatgctcg taaatggttt ccgccaatgc cttgctgatg
      181 ccttccactt tttccaagtc ctcgcggctg gcggcaatca cgccgcgcag accgccgaag
      241 cgggtgagca gggcttggcg gcgtttgctg cctacgccgg ggatgtcgct taaggaggag
      301 gtaacgcggg ctttgtcgcg ttttttgcgg tggccggtaa tggcgaaacg gtgcgattca
      361 tcgcgtacgg tttgcaatag atgcaaggcc gggctgttgg gcggcaggcg gaagacttcg
      421 ccggtaaaag gcagtatgag ttcttccata ccggctttgc gttcggggcc tttggcgata
      481 ccgaccaaag gaatgtgcag cccgagttct tcccataccg atacggcgat gccgatttgc
      541 cctttgccgc cgtcaattaa cacggcatcc ggccatttga cggtctcgcc gttggcttcg
      601 gcttcctgca ttttgccgta acggcgcgtc aacacttcgc gcatggcggc gtagtcgtcg
      661 ccgggtttgg cggtcgtgat gttgtagcgg cggtattgcg agggctggat gttttgctca
      721 tcgtacacga cgcaggaggc gatggtggct tcgccttggg tgtggctgat gtcgaagcat
      781 tcgaggcggt tgaggccgtc tgaatccata ccgaggattt tggcgagttc gtcgatgcgg
      841 tgttgctggt tgctttgttg caggcggcgt tgtgcaatcg ccatttgtgc gttttgttcc
      901 gccattttca accaaacttt gcgttcgcct atggtcttgg tgacaaattg catttgtttg
      961 ccgtgttcgc cttccaaagc ctctttcagc gcatcgggaa cggaaaagtt gctaataatg
     1021 atgtcgggtt tgcttttgcc caaataatgt tgggcgacga aggcttcggc gtaatcttgt
     1081 ccgtttggct cgggatcgtt tttggtgtcg gggaagaaac ttttgtctcc gacgtgccgc
     1141 ccaccgcgta tgctgaccca gtgtacgcag acgatgccgt ctgaaaccgc cagcgcgagc
     1201 aaatcgatat cgttggggtt gttcgggttt ttgctgtcga tgaactggtt gctctgcata
     1261 atgccgagtg cttggatttg gtcgcggtag cgggcggctt cttcaaattg cagattcgcg
     1321 gcggcagttt gcattttgtg ttgcagggtg cgggtcagtt cgtcggtttt gccattgaga
     1381 aaagtggcgg cttgacgcac gctgtcgcgg tagtcttctt cgctgatgtg tccgacgcag
     1441 ggcgcggtgc agcgtttgat ttgatacagc aggcaagggc ggtcacgatg ctcgaatacg
     1501 ctgtcttcgc aggtacgcag cataaagact ttttgcaaca cttgaatgct gtcgcgcacg
     1561 gcgttgctgt tgggatacgg gccgaagtat tggtttggct ttttcagcgt gccgcggtaa
     1621 tacgccatct gcggatattg atggccgctg agcatcaaat aaggatagct tttgtcgtcg
     1681 cggaaaagga tattgtattt cggcgacaag gctttgatga ggttgttttc gagaatcagg
     1741 gcttcggctt cggaacgcgt gatggtggtt tcgatatgat gaacctgttt taccatcaat
     1801 gcgatgcgcg gcgaatggtc gtttttttgg aaatagccgg acacgcgccg cttgaggttg
     1861 accgctttgc cgacgtataa gactttgccg ccttcgtcaa aaaaacggta cacgcccggc
     1921 agcttgggca ggtttttgag gaagagcggg atatcgaatt gttccggaga acggttttct
     1981 gtattcatat caattatttg aaaaacggct tcagacggca tatcggcagc ggcagcctta
     2041 tccgttcgga aaagctgtcg ggtggttata tgcggtcaat cgatttttac ccgtttcagg
     2101 cgcaaggcgt tgctcaacac ggaaaccgag cttgccgcca ttgccgcgcc tgcgatgaca
     2161 gggtttaaaa aaccgagcgc ggcaagcgga atgcccaaaa tattgtagaa gaaggcgaaa
     2221 aacaggtttt gcttgatgtt tctcaacgtc gcccgcgata ccgatagggc atcggcgagt
     2281 tggttgaccg aatgttgcat cagcgtggcg gatgcggtgt gttcggcaac gtccgcgccg
     2341 cctttcatgg cgaagctaac gttggcggcg gcgagcgcag gtgcgtcgtt gatgccgtct
     2401 ccgaccatcg ccacggtttt gccggcggct ttgagtttct gcacttcggc ggctttgtcg
     2461 cgcggactca tattaccgaa ggcgtgtgcg atgcccagtt gtttggcgac gtattcgacc
     2521 gtgccttggt tgtcgccgct cataatatag acatcgatat tgtgtttttt cagacggcct
     2581 atggcttcgg cggtatcggc tttcaacgcg tcggcaagtg cgaatgcgcc gatgggtttg
     2641 ttatcgactg agaccgcaac aatgcttgca atatcccaaa cgccgtctaa aaacttcggc
     2701 aaggccagtt cggcaaattc ggctttgcct gctttcacca aacccacgcc ttccacttcg
     2761 gcggtaatgc ctgcgccgac aacggtttgt gcgttttgtg cggcgggaat gtccaaaccg
     2821 cgcgcttggg cggcggagac gatggcacgg gcgagcggat gggcggcgtt ttgttcgacg
     2881 gcggcggcga tgcggtacaa agcgtcttcg tcaaagccgc tgtcgggaac gcaataaacg
     2941 gcggcaacct gcgggctgcc ttcggtcagc gtaccggttt tgtccaacac gacggcatcg
     3001 acgtgggcgg cttcctccat tgctgccgcg tctttaaacc aaataccgtg tttcaccgct
     3061 ttgccaatgc cgaccataat cgcggcgggt gtagccagac cgagcgcaca cgggcaggca
     3121 atcaccaaaa cggcgacggc gtgcatcagc gcaaccgtcc aatcgccctt aatcagccaa
     3181 gtaacaataa aagtcaacaa cgcgatgccc acgacggcag gcacgaacac cgccgccgcc
     3241 ttgtccgcca cgcgcgcaat cggtgcttta ctgccttgcg cttcagagag tgcgttcatc
     3301 atgtcgccga gcagggtttg gctgccgagc tgcgcggcgc ggtacaccac gctgccttca
     3361 gtcatcagcg cgcccgccaa tactttgccg cctgcctttt tctcttcggg attggattcg
     3421 ccggtaagat ggctttcgtc cgcccagccg ctgccgcttt ctatgatgcc gtcggcggca
     3481 atgcgttcgc cgtgattggc gcggattagg tcgccgattt gcacttggtc gatgggtagc
     3541 tgccgccatt cgccatcgcg ttgcacgttg acttgggttg gcgtgagttt gagcagcaag
     3601 cccaagctgt tcaggctgga ttttttggtg cggtgttcca aaaatttacc cagtgacaca
     3661 aaaccaatca ccattatgcc tacttcaaaa tacacatgcg ccataccgta cgccgcgtgc
     3721 gggctgaaaa acagcatata gacggaatac aggtaaatcg agaccgtgcc gatggtaacg
     3781 agtacgtcca tattcgccag cccgccttta atgctcgccc acgcgctttt gtaaaatggc
     3841 accgccagcc aaagctgcac cacactcgcc aatgcaaact gcaacaaggg cgacaacatc
     3901 caatcatgcc gtgtccaatt cagccctttt agcatcatcc ctaccatacc gataaggaac
     3961 gggatattga tggccagcaa aagccacaac ctccagccga tatggtgttc tgcttcgggt
     4021 tgcggcaatg tatcttccgt cttttccttc gcgccgtaac cggttttctc aatgattttg
     4081 gcaatgtcgg cagcggaagt tttgctgtcg tcaaacgtaa cctgcgcctc ctcgctggcg
     4141 aagttcactc ccgctgattt gacaaagtct tttttgttca acactttttc aatgcgcgaa
     4201 gcacatgcct gacaggtcat gccttcgatt tggaaacgga ttttttgttg cataagcgtg
     4261 attcctgctt tgatattcag acggcagtta aggctgcggg atgccgtctg agcgccggta
     4321 tggtcgaaat ttctcaaacg cgccgataat tcgagtagaa tccgaaaact tgacacagca
     4381 aaggaaaaac aatgtcccaa caccgcaaac tgattatttt gggttccggc cccgccggat
     4441 acaccgccgc cgtctatgcc gcgcgtgcca atttaaaccc cgtcattatt acaggtatcg
     4501 cgcaaggcgg gcaactgatg accaccaccg aagtggacaa ctggcctgcc gatgccgacg
     4561 gcgtgcaagg gccggaattg atggcgcggt ttctcgccca cgccgaacgt ttcggaacgg
     4621 aaatcatttt tgaccaaatc aacgccgtcg atctgcaaaa acgcccgttt acactcaaag
     4681 gcgatatggg cgagtacact tgcgatgccc tgattgtcgc aacaggcgcg tccgccaaat
     4741 acctcggttt gccgagtgag gaagcgtttg caggaaaagg cgtttccgct tgtgccacct
     4801 gcgacggttt cttctacaaa aaccaagatg ttgccgtagt tggcggcggc aatacggcag
     4861 ttgaagaggc actctacctt gccaatatcg ccaaaaccgt taccctgatc caccgccgca
     4921 gcgagttccg tgccgaaaaa atcatgatcg acaaactgat gaaacgcgtg gaagagggca
     4981 aaatcatcct caagctggaa agcaacctgc aagaagtact gggcgacgat cggggcgtaa
     5041 acggcgcatt attaaaaaac aacgacggtt ctgagcaaca aattgccgtc agcggcattt
     5101 ttatcgccat cgggcacaag ccgaataccg atattttcaa aggtcagttg gaaatggacg
     5161 aagccggtta cctgaaaacc aaaggcggca cggcggacaa tgtcggcgca accaatatcg
     5221 aaggcgtatg ggcggcgggc gacgtaaaag accataccta ccgtcaggcg attaccagcg
     5281 cggcttccgg ctgtcaggcg gctttggacg cggaacgctg gctgggcagc caaaacattt
     5341 gaatgccgtc tgaaaccgaa attttcggcg gaatgcaagt ttttcgcacc aaatccttga
     5401 ttccgtcggc aaaccccaat ataatgcccg ttctttgaac cttgccttct gctttcgcat
     5461 ggcagaaggc tgtttttacc atccataagg agataacatg cgtcattacg agatcgtgtt
     5521 tatcgttcat cctgatcaaa gcgagcaagt gcccgctatg gttgaacgtt acaaaaccat
     5581 gattgctgaa gcaaacggta agattcaccg tttggaagat tggggtcgcc gccaactggc
     5641 ttacccgatt aacaaaatcc ataaagccca ttacgttttg atgaacatcg aaaccactcc
     5701 cgaagtggtt gaagagctgg aaaccgcatt ccgcttcaat gatgccgtat tgcgccatct
     5761 aaccatcaaa accaaacacg ccgttaccga agcatcccct atgttgggtg gtgaaaaggc
     5821 taagaacctg ttgagcggtg cgtctgaaga agcggtcgcc caataattgg gattcaataa
     5881 tcttgtttcg cttgccgcgt taattgaaaa ggttttccct attcgatata cgcctgccgg
     5941 aatccctgtt ttagatatta ttttaaagca caaatcgtgg caggaggaaa acgggcagca
     6001 atgccttgtc caattggaaa ttccggcacg gattttaggc aggcaggcgg aagagtggca
     6061 gtatcggcaa ggtgtatatg ttcacgtcga aggtttttta gctcaaaaaa gcagacgttc
     6121 ccttatgccg atgctcagga tacaaaatat tcaagaatat aaaggttaaa cgacaatggc
     6181 tcgtcaatca ttcaaacgta gaaaattctg ccgtttcacg gctgaaaaaa tccaagaagt
     6241 cgattacaaa caagttgatt tgctgaaaga ctttatctct gaaaacggta aaatcattcc
     6301 tgcacgcatc acaggaacga aggcattcta ccaacgccaa ttggctgttg ccgtaaaacg
     6361 cgcacgcttc ctggctctgc tgccttacac cgaccaacac aaataatttt ggagattgaa
     6421 tcatgcaaat tattctgtta gaaaaaatcg gcggtctggg caacttgggc gacatcgtaa
     6481 ccgttaaaaa cggctacgcc cgcaactttc taattcccgc aggtaaggca aaacgtgcga
     6541 ccgaagcgaa tatgaaagag tttgaagcac gccgtgccga attggaagcc aaacaggctg
     6601 aaattttggc agatgcccga gtccgtcagg aaaaattgga cggtcaaacc gttaccgttg
     6661 ctcaaaaagc cggtgtggac ggccgcctgt tcggttccgt taccaatgcc gacattgctg
     6721 ctgcaatcgt tgctgccggc atcgaagccg tgaaagcaaa tgtacgtctg ccgaacggtc
     6781 ctctgaaagc cgttggcgag tacgaagtgg aagtggcttt gcacactgat gccgttgcta
     6841 aaattaccgt tgccgtcgtt gccgcaaccg agtaattgaa attatcagat gccgtctgaa
     6901 accctgccga ttgtcggaat gtggtttcag acggtatttg ttttgttttt cgtatattct
     6961 gcaaaaatcg ggatggttgt ttttatagtg gattaaattt aaaccagtac agcgtacctg
     7021 gcttcaatat actcaatagg ataggtctct tcctaaccga aaaaataatt gtcttttccc
     7081 ttgcaaataa ttgaaatcaa cagattacta aaacacagcc aaaatattca attgacaaaa
     7141 acttttctga ttttgagaaa tctgtgaaaa tcaggggatt atttattttg aaaatggtat
     7201 cgggcataaa atttggctgt gtttcaatat ggtgctgata acatactttg tttcaaaatg
     7261 ttcaatagga taacttcctg attgaccgaa aaataatggc cttttccctt gcaagcagtt
     7321 gaaatcaaca gattgtcaaa atacatccta aaaatttaat aacttatata tatgattgca
     7381 aaggttacgg cttgtatgcc ggtcattatg gcggaaatac cgtttgggag gcagccgcat
     7441 cgcgtggaat cggcacggca ggcgatgcgc cgatgccttc gggcgtgaag gcggggcaat
     7501 cggcagtttg gaaaaaggca atggaatctg tgggcagaag aagttaaggg taaaagtcgg
     7561 gcgcacggtc aggttgtctg tccttttgtc ggaacaggag actgggcagc tttgtcctga
     7621 ttaattgctt gtgcaaacgg taaaaaggtt ttttcgtgtg ttttaaagca ggttgtacag
     7681 catttttccg gcaatcaaaa gcaacataat gccgaaggat tttttgagtt tggcagaaga
     7741 aagtttgtgg gcggttttga caccgagcgg ggcaaaggca atggttgccg cgctgaggac
     7801 ggcgacggcg ggcaggtaaa ggaagcccag tgacccttcg ggcaatcctg caatattcag
     7861 gccgttgagc agatacgata ttgcgccgga gagtgcaatc ggccaggcaa ggccggatga
     7921 tgtgccgatg gctttatggg cggggaagcc gcagtggatt aagaagggga cggaaagtga
     7981 accgccgcct atgccgaccc agctcgacat tgtgccgaac agtgtggaaa ccgcagtcag
     8041 tccgggcagt ccgggcagcg ggcgggatgc cgtctgaggg tcggtatgca gtgttttgaa
     8101 tgcgacggcg gttaaaaaca ggatgaagaa aatttgaagc ccgaacgctg ggatatattt
     8161 tgcggagagt gcgccagcga atacgccgaa taccataccc ggcatcatcg taaatacggt
     8221 tttccagtcg accgcctgtt ttttgtgctg ccccagcata ctggaaaagg cggtgaagac
     8281 catgacggcg aaggatgtgc cgacggcgag gtgttgcgcg taaggatgtt gtgccaaacc
     8341 ctgcaaatca agcacccata aaacgacagg gacaatcagc gtgccgccgc ctacgccgaa
     8401 caggccggca ataaaacctg ccgcactgcc tacggcaagc aggattaaga taatgtccca
     8461 atgccacatt taatccgcct ttctgtattt tttccagaat cggcgtgccg ccataatgta
     8521 gccggacagg ctgtatccga gaaagaacag gaagaggacg agcgacggtt cccaagtgac
     8581 cagaagcagc agtaagacgg caagcagcat tccgacaaag gggacttgtc tgcggatgtt
     8641 gatttcttta aaactccaaa aagggatttg gacaatcatc gacaggccgg caaacagtgt
     8701 gatgcccaat gcccaccagt ggacggcggg gaatttttcg acgctgtggt tgacccaaat
     8761 cagcccgaca atcagcgcgg cggcagtcgg actgggcacg ccgataaacc agcgtttgtc
     8821 caccttgccg atgagcgtgt tgaacagcgc aaggcgcagg gcggcgcagg cgcagtagat
     8881 gaaggcgacg gaataaccga ttttgccgaa ctgccaaagc tgccatttgt aggcaatcag
     8941 agcgggagca acgccgaagc tgaccatatc ggcaaggctg tcgagctgct ccccgaacgc
     9001 gctttggctg ttggtcagcc gcgccacgcg cccgtccata ccgtccagca acatagagat
     9061 gaataccgcg atggcggcgg tttcataacg tccgtgcatg gattgggtga ttgcgtaaaa
     9121 tgcggaaaac agcgcggcga tggtaaagga gttgggcagc aggtagatac tgttttgacg
     9181 cagggagcgt tgcggggtgt tggtcggggt ttccatagct gaagtgtctc acggacgtgg
     9241 agaatcgata ggggattata acccaaacca ttatcgaaca atacgccctt taggatgaag
     9301 ccctcggagg cggtttgttt ttctgatggc ggatgctttg cggggcggtt tgactctgcc
     9361 tgccaccggg tgtgttgttt cggatttttg ccggatggat attgttgccg ggcatttctg
     9421 ggcttgccgg aaggtatcgg ggggagggca tcggaatatg cggcgcaccg ttcaaaccgg
     9481 ggcggcgttt ccctattttc agggggttgc aggtttcaac aaattgaaag atattttttg
     9541 catttccgac cgtatgctga ttttgaagcg ggataaatta taggacaagg tttttcgccc
     9601 gcggcaagga ttgcccgcaa tgccgtctga aaacaacggc ttgccgctaa ggcggcggta
     9661 tgcggatttg cgctgccgaa atcggtttgc catacccggc ggtgcaatct gcggggaggg
     9721 ataagccgat aaggcgggac ttatcctgtt ttttgtttat aatgcaacag aatttggacg
     9781 aaccgtcggg aatttatcat gatgaaaaag atactggccg tatcggcact atgcctgatg
     9841 actgcggcgg cacgggctgc cgatacttac ggctatctcg ccgtttggca gaatccgcag
     9901 aatgcaaacg atgttttgca ggttaaaacc acaaaagaag attcgacgaa aagcgaagcg
     9961 tttgccgagt tggaagcttt ctgcaaaggt caggacacgc ttgcgggcat tgccgaagac
    10021 gagccgaccg gatgccggtc ggtcgtgtcg ctgaacaata cctgtgtcgc gctggcatac
    10081 ccgaaagcct tgggcgcgat gcgcgttgaa aacgccgttg tgattacttc tccgcgtttt
    10141 acgagcgttt atcaggtcgc actcaaccag tgcatcaaaa aatacggcgc acagggacaa
    10201 tgcggcttgg aaacagtgta ttgcacgtct tcttcttatt acggggggaa ctgtgcgctc
    10261 tttgattcaa aatctcaaat aaaacgggaa atgccgtctg aaagatgttc agacggcatt
    10321 tctatatcga cggtcaggat tctttcggat cgggcagcag gctgttcaac ataatggcaa
    10381 gtacggcgca caagcccacg ccggcaaagc tgaagctgcc caatttgagc gtcatgccgc
    10441 cgatgcctgt ggtcagtacc gagctgacga tgaccaggtt tttcggcagc atcaaatcga
    10501 ctttggcatc aatcagggtt ttgatgccta aagaagcaat cgtgccgaac agcagcagca
    10561 taatgccgcc cattactggc atcggaatgg aagccaaaaa cgcattgaat ttgccgaaaa
    10621 acgccatgca gacggcaaaa accgccgccc aagtcatgat gacggggttg ctgtttttgg
    10681 taatcatcac cgcacccgtt acttcgccgt aggtcgtaac cggcgggccg ccgatcagac
    10741 ccgcaacgca tacgcccaaa ccgtcgcctg caagggtttt gtccaagccc gggtctttcg
    10801 tatagtcttt ccccgtcaca ttgccgattg ccatgatgcc gccgatgtgt tcgatggcgg
    10861 gggcgacggc aacgggcagc ataaacagtg cagcctgcca gttgacctga ggcgtttcaa
    10921 aatggggaac ggcgaaccag ggcgcgtgtg caatgcttgc cgtgtccacc agtcccatca
    10981 gcagtgccaa aacataaccc gaagcgacac cgatcaagat aggaatcagc ttcatcatcc
    11041 tgctgccgaa aaccgatacg atggcggtaa cggcaaaggt aaagccggaa agaatcagcg
    11101 aatcggtata gtcgatgacc tgtttgccgt ccgcctgacc cattgccatg ctgcttgccg
    11161 ccacggcaac agacagaccg atgaccatga tgacggggcc gatgacgaca ggaggcagca
    11221 gtttgtgtac cgccgccagt ccgcgccaac ggatcagcgc ggcaaacaca aaatacataa
    11281 agccggcggc aaacagtccg aacatggtgg aaggcagccc ccattcgccg acggagtaga
    11341 taatcggtgc gataaaggca aacgaagaac caagaaaaat cggcactttg cgtttggttg
    11401 tgatttggaa cagcagcgtt cccaagcctg cgcccaaaag cgcaagagcc ggattcagac
    11461 cggtcagcag gggaaccagc accattgcgc cgaatgccac aaataaaatc tgcgcgccgg
    11521 aaacggcaag tttcagttgg ttcataccat cttctttcaa tcggttcaaa cggcacggat
    11581 tatactggtt tgccgagggt ctgcgtaaaa atacatatgg cgggcaacag atatgccgtc
    11641 tgaaaggcaa tgtctgtctg ttgcaggatg aaagcaggag aaatggagta taatccgaca
    11701 ctttccatat tcaatccgca agccgtccgg cttccttatt tcagacggca ttttccggta
    11761 atacggcatg acagaaaaat cccataaaaa aacagcaaaa ggcagggcgg gaagcccgtc
    11821 cccaacgtcc gcacgcaaca agaaagccga caacggcgca cggggcaata aagtttccga
    11881 gcggctcaaa gcggtcaaag agttgcagaa aaccgaaacc aaaaaggcgc gtcccgaaca
    11941 tgtcgtcaac cttatcggcg acgcactgtg gctgatgggt ttggcggcaa ccttgtattt
    12001 ggcgatttcc ctgatcagtt tcgatatggg cgatccgtct tggtcgcaca gttcgccggt
    12061 tgtggaagat gtcgccaatt ggggcggact gttcggcgcg tatgttgccg atgtcggcta
    12121 ttatcttttc ggctggtcgt tctggtggtg gatagcggct gcctgcgtca tgctgtataa
    12181 aaattttcgt ctgcacgcaa aacagacgga aaacgaggca tacaaccaca aaatcgctgc
    12241 cgccgcgctg tttgtcctga cggtcttcag ccccgtcttg gagtattttg tgctgggcgg
    12301 aaaatatgcc gactccctgc ctgtcggagc aggcggtatg gtcggcatac gcgtcggcgc
    12361 agtgtttgcg tggctgctgg ggaaatcggg cagcctgctg attatcttgg ttgttctgct
    12421 gttgtcgttg tccctgctgg tgcagatttc atggctggaa tttttgaacg gtgcgggcag
    12481 ggcggttcaa aaccgcctga gtgccttatc cggcaaggtc atggctttgg gaaaacgccg
    12541 gccgaatacc aaaacagacg gtgtcgatac ccaaaataca cggcgcatgg taaaagaagc
    12601 caagaatatt acggccaaac ccgttgcctt gcccgaaggc agcagcagca accgcaaatc
    12661 cgtcgcggtt tccgtcgcgc cgccgcccaa aattcaggtt tctctgtttg aagatgacga
    12721 acctcggcag gcgggcgaat accacaagcc tacattgaac ctattgcgga ttcctgacag
    12781 cgaacccgtc agcatcaatc ccgccgaatt ggagcgcact gccgaactga tcgaatccaa
    12841 actggcagaa ttcggcatcg gcgtacaagt cgtatccgcc acatccggtc ccgtcatcac
    12901 gcgctacgaa atcgaacccg cgcaaggtgt taaaggcagc caaattgttg ccttgtcgaa
    12961 agatttggca cgctctatgt cgctgcagtc cgtgcgtatc gtcgaaacca tcgcaggtaa
    13021 aaacacgatg ggcatcgagt tgcccaacga caaacgccaa gacgtgatgt tgagcgaaat
    13081 cttgtcctcg cccgtgtttg ccggagccaa atccaagctg accgtcgcgc tgggcaaaga
    13141 cattgccggt acccccgttg tcggcgactt ggcgaaaatg ccgcaccttt tggtcgccgg
    13201 tatgactggt tcgggcaagt ccgtcggcgt gaacggcatg attatgtcta tgcttttcaa
    13261 agctacgccc gacgaagtcc gcttcattat gatagacccg aaaatgctcg agttgagcat
    13321 ttacgacggc attccgcacc tgctctgtcc cgtcgtgacc gatatgcgcg aagcagggca
    13381 ggcgttgaac tggtgcgtcg ccgaaatgga aaaacgctac cgcctgcttt cccacgccgg
    13441 tgtacgcaat ctggaaggct tcaaccaaaa agtcgaagcc gcaaaagcgg caggcaagcc
    13501 gctgctcaat ccgttcagtc tgaaccccga caaccccgag cctttggaaa aactgccgat
    13561 gattgtggtc gttatcgacg aacttgccga cctgatgatg accgaacgca aagccgtcga
    13621 gcagcaaatc gcccgtctcg cccaaaaagc gcgcgccgcc ggtatccata tgattgtcgc
    13681 cacccaacgt cccagtgtcg atgtcgttac cggcctgatt aaagccaaca tcccgacgcg
    13741 tatggcgttt accgtgcaaa gcaaaatcga cagccgtacc atcctcgacc aaatgggcgc
    13801 ggacgaactg ctcaaatatg gcgattcgct gttccttcag cccggcagtg ccgaaccgac
    13861 ccgcctgcaa ggcgcgtttg tttccgatga tgaagtccac cacgtcgtcg ctttcgtcaa
    13921 ggagcaggca ccggcaaatt atgtagaagg gctgcttaca ggcgaagcgg cacaggaaac
    13981 ggcaaatatc gtcagcccca atgcagacag tgacgaattg ttcgatcagg ctgtcgccta
    14041 tgttttggaa agcaaaaaaa cctccatttc gtctttgcag cggcagttgc gtatcggcta
    14101 taaccgcgcg gcaaacctga tggaggcact ggaaaatgca ggtgtcgttt ctccctccga
    14161 tcttaacggc agccgtaaaa ttttggcgca caaggaccat ttatagccca tatcgcaaaa
    14221 tgccgtctga acggtggaat tggcgtttca gacggcatat tatgtttcag gcgaaacatt
    14281 gtgatatact tgccagctaa aatttcccct ttgcggcaat gcggttcaaa tatcgtaccg
    14341 ttgcgctgtt tgcttcccca tgtagggaag aaagtttatc attttatcaa cacaacaaat
    14401 ttaagggctt atgatgagcg taactgttga aactttagaa aatctggaac gcaaagtagt
    14461 gttgtccctg ccttggtccg aaatcaacgc agaaaccgat aaaaaactga aacaaaccca
    14521 acgccgtgca aaaatcgacg gtttccgtcc gggtaaagca cctttaaaaa tgattgccca
    14581 aatgtacggt gcgagcgcac aaaacgacgt gatcaacgag ctggtgcaac gccgcttcca
    14641 cgatgttgcc gttgcccaag agttgaaagt ggcaggcttc ccccgttttg aaggcgttga
    14701 agaacaagac gataaagaat ctttcaaagt tgccgccatt tttgaagtgt tccccgaagt
    14761 cgttatcggc gatttgtctg cacaagaagt tgaaaaagta accgcttccg tcggtgatgc
    14821 cgaagtggac caaaccgtag aaatcctgcg caaacaacgc acccgcttca accatgtcga
    14881 acgcgaagcc cgaaacggcg accgcgtcat cattgacttt gaaggcaaaa tcgacggcga
    14941 accttttgcc ggcggcgcat ccaaaaacta cgccttcgta ttgggcgcaa gtcaaatgct
    15001 gcctgaattt gaagccggcg tagtcggcat gaaggcgggc gaaagtaaag acgttaccgt
    15061 caatttccct gaagactacc acggtaaaga cgttgccggt aaaactgccg tgttcaccat
    15121 tacgctgaac aacgtttccg aagcgactct gcctgaagtc gatgcagatt ttgcaaaagc
    15181 cttgggtatt gcggatggcg acgttgccaa aatgcgcgaa gaagtgaaga aaaacgtaag
    15241 ccgcgaagtg gaacgccgcg taaacgaaca aaccaaagaa tccgtaatga acgcgctgct
    15301 caaagccgta gagctgaaag cacctgttgc tttggtcaat gaagaagccg cacgcttggc
    15361 aaacgaaatg aaacaaaatt ttgttaacca aggtatggct gatgctgcca acttggatct
    15421 gcctttggat atgttcaaag aacaagccga acgccgcgtg tctttgggtc tgattttggc
    15481 caaactggtt gatgaaaaca aactggaacc gactgaggag caaatcaaag ccgttgtcgc
    15541 caacttcgca gaaagctacg aagatcctca agaagtaatc gactggtact acgcagagcc
    15601 ttcccgcctg caagccccga cttctttggc gatagaaagc aacgtcgttg atttcgtttt
    15661 gggtaaagcc aaagtaaacg aaaaagcttt gtcttttgac gaagtgatgg gcgcgcaagc
    15721 ctgattatcc tgaaaatgcc gtcttagggc agtttttgaa agcaccgaag ccgcttttaa
    15781 agcccgcttt ggtgctttta catcatgaaa ggagacgaaa tgtcttttga taactatctt
    15841 gtccctaccg ttatcgagca gagcggtcgc ggtgagcgtg cattcgatat ctattcccgg
    15901 cttttgaaag agcgcatcgt attcttggtc ggcccggtaa ccgacgagtc cgccaatctg
    15961 gtggttgccc aactgttgtt tttggaaagt gagaatccgg ataaggatat tttcttctat
    16021 attaactcgc cgggcggttc ggtaacggcc ggtatgtcga tttacgacac catgaatttt
    16081 atcaagcccg atgtatcgac tttgtgcttg gggcaggcag caagtatggg cgcgttctta
    16141 ttgtcggcag gcgaaaaagg caaacgtttc gccctgccca acagccggat tatgattcac
    16201 cagcctttaa tcagcggcgg cttgggcggt caggcatccg atattgaaat tcacgcacgc
    16261 gaactgttga aaatcaaaga aaaactcaac cgcctgatgg cgaaacattg cggccgcgat
    16321 ttggcagatt tggagcgcga caccgaccgt gataatttca tgtctgccga agaagcaaaa
    16381 gaatatggtt tgatcgacca agttttggaa aaccgcgctt ctttgcagct ttaaaccttg
    16441 tgaagtagac aaatgccgtc tgaaaacagt tcagacggca tttgtcttat agggactgta
    16501 ttgggatgat tttgttctgg ttgcgtttgg agccgttttc gccgtagttg gttttgacat
    16561 actcttcaac aatagccaaa aattccgttg caatgttgtc gcctttcagc gttactttgc
    16621 gttcaccatc tacataaaca ggcgcgacgg gtgtttcccc cgtaccgggc aggctgatgc
    16681 cgatgtcggc caatttgctt tctccgggcc cattgacaac gcagcccatt acggcaacgt
    16741 tcagggattc aaccccagga taaagggtac gccatataga cattttttgg cgcaggtaat
    16801 tttgaacatc ttgtgccagc tcttgaaata cggtactggt ggtacgcccg cagccggggc
    16861 aggcggtaac catcggcgta aacgaacgca atcccatagt ttgtaaaatc tcttgcccga
    16921 cgacgacctc ctgagtacgc gggctgccag gttccggagt cagtgaaatg cggatggtgt
    16981 cgccgattcc ttcttgaagc aagacggata atgccgccgt tgatgcgaca atgcctttgc
    17041 tgcccatacc ggcttcggtc aaacccaaat gcagcggata ggcgcaacgg ctgcccagtt
    17101 cgcggtaaac ctgaatcaaa tcctgaaccg cgctgacttt gcacgacagg atgattttgt
    17161 cttcgggcag tcccaataga acggcttttt cggcagattc caaagcggag acaatcagtg
    17221 cttccttcgt cacttcttcg ggcggtttcg gcgcggaaga agcgaggttg gcatccatca
    17281 tacgtttggc gaggctttga tccaaagaac cccagtttac gccgatgcgg acggctttat
    17341 cgttttcagc agcagtccga atcataaagg caaatttttc atcgcctttt acgcctttgc
    17401 cgacattgcc gggattgatg cggtatttgg acaatgcttt gccgcattct ggaaattccg
    17461 ccaacaggcg ttcgccgttg aagtggaaat cgccgataag cggtgtggca tagcccatat
    17521 cgtccaagcg gcggcggatt tcggcaactt tggacgcggc ttcggggctg ttgacggtaa
    17581 tacgcaccat ttcggatccg gcgtcgctca attccttaat ctgcaatgcg gtggcttttg
    17641 catcggcagt gtcggtgttg gtcatagatt ggataacgac gggtgcttct gaaccgacgg
    17701 taatatgatc gatgcggact tgatgcgtct tgcggcgttg gagtgtgttc atatggtttg
    17761 aatctgttta ttgaccgatg aggacggttt gcaattcttc cgagtagggg aaattcgcct
    17821 gcaattgtgc ttcatattcg tatgccgcct gtgcgttgcc gagggctttg gcaattttcc
    17881 agcctagcag caaatcatcg gcctgaagga cttctaccct gctttggtat tttttaaagt
    17941 agtaatcggc atcgcccaac tgcccggcca gcattttggt gcgcgccagt tctttaaatg
    18001 cgggtgggaa ctgcggctgg gcggcgaggg aacgtttcaa ataggcttcc gccaatccga
    18061 attgcccctg ttttgcgctg catatgcctt tattcaggtt ggcaatataa ggggtcgggt
    18121 aggtggggtc ggccagggct ttgtcgaaat atgccataga ttcggcaggg cggttgagcc
    18181 tgccgcacag gaaccagccg tagttgttgt tgatttcggc actgtcgggt ttgatggaga
    18241 gggcttgccg gaaactttcc tgcgccttgt cgttaacttt caggtattga tagatttcgg
    18301 cacggaccag ccaggcaagc tcgtttttag ggtctgattt caaggcgtct tcaatacttg
    18361 ccgtcgcctg acggtagtcc tgaccgcgca tatattccat tgccaactgg gttttgatat
    18421 tggaaacctg attggctttt tctgcccgcg aggggcggta ggaagtgctg cacgcgccca
    18481 aggcaaggac aagtaataaa gagattcgtt tggatggctt aaaaggcata attacccctg
    18541 ttgtccgatt aaaatctgct gccatttttg ttggcggcgc gttttatcct gaacctgccc
    18601 cgccaactgt ccgcaggcgg catcgatgtc gtcgccgcgc gtttttcgta cggtaacgac
    18661 aaatcctgcc tgctgcaaaa tatcgcggaa cacacggatg ttctcattgc tggagcgttc
    18721 gtatccggag tttgggaagg gattgaacgg aatcagattg aacttgcagg gaacatctgt
    18781 gaccagttcg atcagttcgc gcgcatgttg cgccttatcg tttattccgt ccaacatgac
    18841 gtattcgaaa gtgatgaaat ccctgggtgc tttgaccaga tagcgttggc atgcggccat
    18901 caattctttc aagggatatt ttttgttcaa cggtacgatt tggttgcgga cttcgtcatt
    18961 ggaagcgtgg agggaaaccg ccaaagccac cggcatgaca tcgcgcaacc tgtccatttg
    19021 gggaaccata cccgaagtgg aaacggttac gcggcggcgg ctcaaaccgt agccgtggtc
    19081 gtccagcatg atgcttaagg cggtaacgac attgtcgaag ttcgccatcg gctcgcccat
    19141 acccatcatc actacattgg aaattacgcg ttcgtttttc ggtgtaacac ccatcgcttt
    19201 gtttgcccac cacaattgcc cgatgatttc ggcagcagtc aaattgcggt tgaagccctg
    19261 ccggccggtc gaacaaaatg tacattccaa agcgcagccg acttgtgagg aaatgcagag
    19321 cgtgccgcga tccgattcgg ggatgaagac ggtttccacc ccgttgcccg taccgacatc
    19381 caaaagccat tttcgagtgc cgtctgaaga tttttgagac atcatcagct tgggaatttc
    19441 gatgcctgcc tgttcgttca gtttatggcg caacgatttt gccaaatcgg tcatttcgtt
    19501 aaaattttgc gcgccggatt ggtgcatcca acgcataacc tgtttggcac ggaagggttt
    19561 ttcgcccata tcggcaaaat gtcgggtcag cccttgaagg tcgtagttga gcagattggt
    19621 tttcatgtgt ttgtgttttt cttaaatcaa ggcttcaaat aaaaaattgc agggcaggca
    19681 agtttgattt gaagcctttt gcaacagagt cttcagacgg catggatata ttttatgccg
    19741 tctgaagagg agttgggcgg tgtattgtat caacgggggc agatttcggt ttggctgaaa
    19801 aagtaggcaa tttccaaagc ggcattttcc acgctgtcgg aaccgtgtac ggcattaatg
    19861 ctgaccgaag tggcaaagtc cgcgcgtatc gtgccttcgg cggcttcgga aggattagtt
    19921 gcacccatca gttcgcggtt tttcaggacg gcgttttcac cctctaatac ctgaatcata
    19981 accggaccgc cggtcataaa ttcaaccaat ccggcgtaga aggggcggtc tttatgaacc
    20041 gcataaaatt cttgcgcctc tttgagggta agctgcttca ttttggcggc aacgattttc
    20101 agaccgttct cctcaaagcg gctgtatatt ttgccgataa cgtttttgcc gacggcatcg
    20161 ggtttgatga tggagatggt acgttcaatc gccatgctat atccttattt ttacttaaga
    20221 agaatcaaat cggtattcta tcaaaaaaat aacttatccg cctgaagcct tgggagatag
    20281 ggtcaggtgc ggctgttcca aataaacttc ggactgcatt tcgaccatcc ggctggcggt
    20341 gcgggtaaat tcttcggcaa aatcgccttc cgtatagata tggttgacat ctaccgcgcc
    20401 ggtggcacac agtttgaccc ggaaatcgta gagtacgtca atcagccaag tcagccgccg
    20461 cgcctccgcc ttttcttgcg gtgagagttg ttccaaacct gaaataaaaa ccatttcata
    20521 atgttcggcc aaatacagat agtcggactg tgagcggggg ccgaagcaca gtgcgcggaa
    20581 atcaaaccat atggcacggc cggactcggc tttgtgggga atctcccgac catggatggt
    20641 gctgatgccg gggttcaaat cggtaatgcc tgtcatttct ttgaacagtt ttgccagttt
    20701 tgcctcattt tcttcattgg caggcgtaaa gaaaatctcg gcggggcgga gggtacgcag
    20761 tcggtagtct tcaccgccgt caacgtttaa gacggtcagg ctggactcga tgagcgcgat
    20821 tgtgggaaga aaactgctcc ggttttgacc ttgcgggtag agttcggaag gcgcgtagtt
    20881 tgaagtcgcc accaaaacaa cgccctcgtt aagcaggttt tccagcagac ggcctaaaat
    20941 cattgcatcc gcaatatcgc tgacatgaaa ttcgtcaaaa cacaatacgc gggtttcttt
    21001 ggcaatctcg gcggcaacgg atttcaacgg gttgctttcg cttttcaggg ttttcagcct
    21061 ctggtggatt tctgccataa aggcatgaaa gtggacgcgg cgtttgcggc ggtaagggag
    21121 gcagccgaaa aaagcgtcca tcagaaagct tttgccgcgt ccgaccccgc catagaaata
    21181 aagccctttg gggacttgcg gggaacgcaa actcctgcct aaaaaacggt ttcttttgcg
    21241 tttgaacatc atcaattcgg tccaaagccg atcgaggtgt tcgatggcgg ctgcctgcgc
    21301 gtcgtcgcgg atgaagttgg gcagttgtga ggcagcctga taccaagtca gcgggctgtg
    21361 attttcaaac ggcggggctt taaaaagttg gtctctatta ctcatattta accttgttat
    21421 acttcttgcg gcggagaaag aaaatgctta ccgtcggtat tataaatgat ttccttcaaa
    21481 cttggcgttt tccgtggaaa agaataatga cgcgcacaag ggggggtatg agcaaaggcg
    21541 gcgtttcaat atggtgcaga tatgagctca aatgcctttt atagt
//
LOCUS       DAWKOD010000016        21489 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000016 DAWKOD010000000
VERSION     DAWKOD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.016"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..814)
                     /locus_tag="WJK73_000442"
     CDS             complement(68..814)
                     /locus_tag="WJK73_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7556974.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WJK73_000443"
     CDS             complement(993..1664)
                     /locus_tag="WJK73_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ7556975.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDVNGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGRGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJK73_000444"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJK73_000444"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ7556976.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WJK73_000445"
     CDS             complement(2481..2963)
                     /locus_tag="WJK73_000445"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ7556977.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WJK73_000446"
     CDS             complement(2995..3684)
                     /locus_tag="WJK73_000446"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ7556978.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJK73_000447"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJK73_000447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ7556979.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WJK73_000448"
     CDS             complement(4571..5881)
                     /locus_tag="WJK73_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7556980.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSEKVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJK73_000449"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJK73_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ7556981.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WJK73_000450"
     CDS             complement(6056..6355)
                     /locus_tag="WJK73_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556982.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WJK73_000451"
     CDS             6706..7902
                     /locus_tag="WJK73_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7556983.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8538..10379)
                     /locus_tag="WJK73_000452"
     CDS             complement(8538..10379)
                     /locus_tag="WJK73_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ7556984.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10470..10940
                     /locus_tag="WJK73_000453"
     CDS             10470..10940
                     /locus_tag="WJK73_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ7556985.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11011..11823)
                     /gene="hpnC"
                     /locus_tag="WJK73_000454"
     CDS             complement(11011..11823)
                     /gene="hpnC"
                     /locus_tag="WJK73_000454"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ7556986.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRCAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11910..12392)
                     /locus_tag="WJK73_000455"
     CDS             complement(11910..12392)
                     /locus_tag="WJK73_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7556987.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12865..13161
                     /locus_tag="WJK73_000456"
     CDS             12865..13161
                     /locus_tag="WJK73_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7556988.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13271..14638
                     /locus_tag="WJK73_000457"
     CDS             13271..14638
                     /locus_tag="WJK73_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7556989.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGERVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWTLRENISASQRKLGTSIKHD
                     IAVPIGHVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPDILSNEAYRYED
                     DINSTVYRNVLACNGTVAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14819..16855)
                     /locus_tag="WJK73_000458"
     CDS             complement(14819..16855)
                     /locus_tag="WJK73_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ7556990.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSK
                     LTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKK
                     ISFYDKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGN
                     SGPRLIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLAGWEEHLYSGLDPFH
                     YITVTDNSHVIGQTISLDQFILENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKE
                     DYAGGARFRFAYDPKEAKNTALIFEKNVTGTSNIIFENPIDDLKSLDGHQIIKVNGTA
                     DKRAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDK
                     NSDIFDRTLPRKGLWLRLISGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLS
                     VGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQR
                     FRHRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVN
                     GKFSDSENAHVNLLGSRQLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEM
                     DGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQTKQGALNLQWTF"
     gene            complement(17221..17667)
                     /gene="smpB"
                     /locus_tag="WJK73_000459"
     CDS             complement(17221..17667)
                     /gene="smpB"
                     /locus_tag="WJK73_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ7556991.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17722..18732)
                     /gene="waaF"
                     /locus_tag="WJK73_000460"
     CDS             complement(17722..18732)
                     /gene="waaF"
                     /locus_tag="WJK73_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ7556992.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18863..18938)
                     /locus_tag="WJK73_000461"
     tRNA            complement(18863..18938)
                     /locus_tag="WJK73_000461"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18903..18905),aa:Asn,seq:gtt)
     gene            complement(19129..19881)
                     /locus_tag="WJK73_000462"
     CDS             complement(19129..19881)
                     /locus_tag="WJK73_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ7556993.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20192..20959
                     /locus_tag="WJK73_000463"
     CDS             20192..20959
                     /locus_tag="WJK73_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7556994.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKPLRQHRRRPVRSHIGGN
                     QFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRRIVRHFFIRQHRRRIIKRRAHPPG
                     VRQLHRIAHCLRRPVLCGEPFYGFLFKSNPLRLIEAV"
     gene            complement(20908..21187)
                     /locus_tag="WJK73_000464"
                     /pseudo
     CDS             complement(20908..21187)
                     /locus_tag="WJK73_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT         5348 a         6166 c         4925 g         5050 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg ctacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc caacacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg ccggacgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccctgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaagcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcac gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctaccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgca
     1381 tcgatatacg ccatacggat tttgtgcttt gcgccgttaa catcgataac gtgcagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt gaccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccgccgccc ttaatcattt gcagggcgtg gttcacttcg tccgcaccgt
     2161 cgatatagac cgccaaaccc gatacttcgt tcagggaaac gacgggaatg tcgtactggg
     2221 caagcagttc gctggatttt ttggaagtag ataccgcgcc tttgattttt ttaccgcttt
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cagcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acatttattt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tacctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgtt tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccaca
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaagt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc ttttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacaggtgct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgcgcctc tgactcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 tgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgttagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctaaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 gccgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac taacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aagattcaat ccccacgcga
     7921 actgcctccg aaaacggagg cagttttttt atccggcttt ccatgcttaa acagcaccgc
     7981 ctcttttcag acgttgacgg ttgcagccgc ttacctgaac cttatagtgg attaacaaaa
     8041 accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca
     8101 ccaagtaaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8161 tttgttaatc cactatatcc gactttccat acttaaacag cactgcctct tttcagacat
     8221 tgacggttgc agccgcccac ctgaacctta atgattgact attttttaat catgttatta
     8281 ttttccataa aatacatggc attaagatgt ttttccacaa aagatacaca caccggcaaa
     8341 caccggctgt gtttatcttt tcttatgcct attttttaat catcgtattt ttatctttta
     8401 atttcaatac gcaaaccaac ttatacacac ggttttcaca tctttggact gcttccgtgt
     8461 gtatagtgga tattgccgtt ttcctttctg acaaaaatgc cgtctgagaa cttcagacgg
     8521 catttgaaac atcggaatca gcggttttgt tcataccact cgataaactt gtctgctttg
     8581 acaaaaccca gcagcggctc gctgcggctg ccgtcggagc ggacgacaaa cacgcccggc
     8641 ggtccgaaca gaccgtattc tttcaacaac gcctgatgtt cgggcgtgtt ggcggttacg
     8701 tcgatttgga aaaagcgttc catatcgact gcctgatgca cttccggctg attgagcgtg
     8761 taagccgcca tttctttgca ggaaatgcac cagtcggcat aaaaatccaa aacgacgggt
     8821 ttgtcgggat gttctttcaa cgccgtatcc atcgctgcct tcagcgcggc agtatcggca
     8881 aacatttttc cgtgttccga agatttgcct gcttcggccg gcggattgag ggtcaggaaa
     8941 tggtgcagcg cggtcgtttt gccgtttgcg ccctgccagc cgaaccacgc gccgcctatc
     9001 agcaatatgc cgcccaatgc gaatgccaca gctttcggac ggcgtttctg cctgcgtccg
     9061 ttgaccagca gcatacaggc aggaaccagc atcagcagcg tgtacagcgc gacgacgaga
     9121 taatagggca agtgcggcgt ggcgaggtaa acggcgacgg caagcaggat aaagccgaaa
     9181 gcgtatttga cggcattcat ccaatcgcct gccctaggca ggatatgccc gccgaacgtg
     9241 ccgatggcaa tcagcggaac gccggtgccc aacgccaaag tgtaaagtgc caaaccgcct
     9301 aaaaccgcat cgcccgtctg accgatgtag cccaaagcaa atgccagcgg cggggcgacg
     9361 cacggcccga caatcagcgc ggacaatatg cccataataa agacggaaac gattttaccg
     9421 cctgaaagcc tgctgctctg gttttggaaa tacgactgca cggcgttggg aagctggatg
     9481 ttgaacagcc cgaacataga cagtgccaag acgaccatca aagccgatgc cgccaacacg
     9541 acccaagcct gctgcaacca tacggtcagc agcgcgcccg tcagtccggc aacaatgccg
     9601 accagcgtat aagtcagagc caaaccctga acataaacga cggacagcac aaacgcccgc
     9661 gccttgcccg cctttttgtc gccgaccaca atactggaaa caatcggcaa caggggatac
     9721 atacaggcgg taaaactcag acccaaaccg gcgagaaaaa acgccaaaag attggcgttg
     9781 agcgtatccc aagacagctt gaaacggctg tcgccgccct catccccctt cgggggcggc
     9841 agcgccccgc tgccgttttg agaggaaggc tgcaaaaagc ggtctttggc ggatgccggt
     9901 tcgtcggttt gcggatggta agtgccgttg ccggaaatat caaactcggt atccacgggc
     9961 ggatagcaca cgccggcttc ggcacagccc tgataggtca aaaccaattt atacggttcg
    10021 ccgacagcct ttgcataagg aaaggcaacc tgcgcttcgt gatggtaaac cgtctgcctg
    10081 ccgaaaaact cgtcttcctt ctcttcgccc ttgctgaaag aaggctgtcc caacaaatcc
    10141 gccggatcgg tcttgccgac gattttcgcc tgatacatat agtatccgtc ggcaatcctg
    10201 aaacggacgt tcacaccgtc gtcggcaacg gcaagctccg gcacgaatgc cttttccggc
    10261 ggcagcagat cgttcgcatc cagcgcgaaa gctcgtccgc acaacatcaa aaatacggcg
    10321 aacaggcaaa tcagtttttt cataatcgaa tccgtttcag acaaataatt tgtctgcatt
    10381 ataaatggta aggttgacgg tgggatttaa tttatgtaaa acccgccatt atccgaacct
    10441 atttccataa acatcttatc gaacccgcca tgtacgatgt caatacccac gatgtccgcc
    10501 gctttttcgc ccgcgtgtgg cagcagcggc tcaatccgct gcaactgagc gcactggaac
    10561 agaaagccct ccgcattgtc gaagcccatc ccgaatacca ccgctatctc gaacgcatcg
    10621 aagaccatct ggacaccgac tggctgcccg aaaacggcga aagcaacccc ttcctgcata
    10681 tgtcgctgca tctgtccgtc caagaacagg cgggcataga ccagccgcac ggcatacgcg
    10741 caatccacga caccctgtgc gccaaacgcg gctggctgga agccgaacac gaaatgatgg
    10801 aggcactggc ggaaacactg tggacggcgc aacgctacgg caccggtttg gatgtcaatt
    10861 tctacatgac ccgactgcgc aaactcatcg gcttgggtgc agaagatcaa gccaggttga
    10921 acccacatga aatcgcctga ccataccaac cgcctgcaaa atgccgtctg aagcggaaca
    10981 acccctttca gacggcatgc atttccccca tcatttccac aacgcgcgtt tcagcataat
    11041 caaccaatcc ttcttatcca aaacggggcg ttgtgcaaac acatcgtatc ggcacgcgtc
    11101 cagtttctgc aaaatcaact gcgcccccaa cacaatcata cggagttcca aaccgatacg
    11161 ccctttcaat tcgcgcgcta aaggcgaacc cgccttcagc atacgaaatg cgcaccggca
    11221 ctcatacgcc atcagccgct gaaacgccgc atccgcccta ccctccgcaa tctgtccttc
    11281 ggaaacaccg aatttcaaca aatcgtcctg cggaatataa accctgccct tttgccaatc
    11341 cacagccaca tcctgccaaa aattcaccag ttgcaaagcc gtacagatgc cgtcgctttg
    11401 cgccacgcac accgcatccg ttttcccgta caaagccagc ataatgcgtc cgacaggatt
    11461 ggcagaacgt cggcaataat cggccagctc gccgaaattt ccataccttg ttttaaccac
    11521 atcctgagaa aatgcagaaa gcaaatcata aaacggctgc aaatccaaac cgaacggcac
    11581 aaccgcctcg gcatccaatc gtgcaatcaa aggatgcgcc gaccggccgc ccgatgccaa
    11641 cacgtccaac tcgcgccgca aaccctccaa ccccgccaac ctggcttcag acggcatact
    11701 gccctcgtcc gccatatcgt ccgccgtccg tgcaaacgca tacaccgcat gaaccggctt
    11761 cctcaacctg cgcggcaaaa tcagcgaacc gacgggaaaa ttttcataat gcccaaccga
    11821 cataccttct ccatccatca aacaaaatgc cgtctgaaac ggaacaaacc cttttcagac
    11881 ggcatcagat acctccaagc tgccggcaat cagtggtggt gatgaccgtg cgggccgtgg
    11941 acatgaccgt gtgcgatttc ctcatcggat gcatcgcgca cgctttcaac cgtagcctta
    12001 aaacggattt tcatgcctgc caaaggatgg ttgccgtcca ccaccgcctt gccgtcggca
    12061 acatcggtta cacgatagac gacaacatcg ccggtttcag gatcgtcggc ttcaaacatc
    12121 atgccgactt cgacttcaac agggaacacg cccacatctt caatacgaac caactccgaa
    12181 tcctgctcgc cgaacgcatc gtcgggcgaa agcgccacat cgaccgtatc gccggcatcc
    12241 ttaccgtgca acgcctcttc caccaaaggg aaaatgccgt cgtaaccgcc gtgcagatac
    12301 gcaatcggtt cttcggtttt gtccaaaagc tgattgttgg catcatacat ctcataatgc
    12361 agcgaaacca cggaattctt cacgatagcc atatttgtcc tttcaggaac agcagattga
    12421 ttacaggcgc attctaacac aaccgccgcg ccggccgatt accgttaacc tgttcataaa
    12481 ctgtacagca catatttcaa tgtaaatttt tgttatttta ttgcgatgta acttttttac
    12541 aacattatta aaaccattcc gacctgtctg ccgactttcc caatccgcct taataaatca
    12601 tacaagatac tgaaattata ttaatctcta taatatttat ccctatcgaa tttttaacag
    12661 caaaaccgtt ttacaggatt tatcaatccg cccgccagaa aacttttcat tcaaaccttt
    12721 ttcccatctg tacgacattg caatccctta ttccatagtg cataattacg caaattcagc
    12781 gatgaatttc caacccggtt tgtagtatgg tcgataaaga cctatttgtt tcaataattt
    12841 aaattggttc taaaggttac taaaatgaaa aaatccctgt ttgccgctgc tttgttgtct
    12901 ttggtcctgg cagcctgcgg cggtgaaaaa gccgctgaag ctcccgctac cgaagcacct
    12961 gccgccgaag ctcccgctac tgaagcacct gccgccgaag ctcccgctgc tgaagcacct
    13021 gccgccgaag ctcctgctac tgaagcacct gccgccgaag ctcccgctgc tgaagctgcc
    13081 gctaccgaag cacctgccgc tgaagctgcc gctaccgaag cacctgccgc tgaagctcct
    13141 gctgccgaag ctgcaaaata agcattttcc gcttgcaaaa aagcaggata cgttcagtat
    13201 cctgcttttt tgatttttca gacggcatca gattcccttc ctcaatcttc tccctaccct
    13261 tccgacaaac atgcctgacc ttcataccga attttcccga ctcctgccgg cagatgaaat
    13321 tgccgaactt tctccgacgc ttttaaaaga ccagcgcaac cgctttacgt ctgcaccaga
    13381 catcattttg cagccgcgca gcgttgaaag cgtgcaaacc attatgcgtt tttgttacga
    13441 acaccgtatt ccggttacgc cgcaaggcgg caataccggt ttgtgcggcg cggcagtatc
    13501 ggaaaacggc gtattgctga acctttccaa actcaaccgc atccgcagca tcaatttgtc
    13561 agacaactgc ataaccgtcg aagcaggttc cgtactccaa accgtccaac aggcagccga
    13621 agcctcaaac aggctgtttc cactcagtct cgccagcgaa ggctcgtgcc aaatcggcgg
    13681 caacatcgcc tgcaatgccg gaggtttgaa cgtattgcgt tacggcacga tgcgcgacct
    13741 ggttatcggt ttggaagtcg tcctccccaa cggcgaacgg gtttcccatc tccatcccct
    13801 gcataaaaac accaccggct acgacctgcg ccatctgttt atcggcagcg aaggcacact
    13861 gggcatcatc actgccgcca cactcaagct ttttgcccgt ccctcggaca aggcaacggc
    13921 atgggtcggc atacccgaca tcgaatccgc cgtccgcctg ctgaccgaaa cccaagctca
    13981 ctttgccgaa cgcctatgca gttttgagct gatcggccgt tttgccgccg agttgtcttc
    14041 cgaattcagc aaattccccc tgccgacaca ttcagaatgg catattttac ttgagttgac
    14101 cgactcatta cccgacagca atcttgatga tcggcttgtc gaatttcttt ataaaaaagg
    14161 atttaccgac agcgtattgg cacaaagcga acaagaacgt atccatatgt ggacactgcg
    14221 tgaaaacatc tccgcatcgc aacgcaaact gggcaccagc atcaaacacg atattgccgt
    14281 ccccatcggg cacgttgccg actttgtccg ccggtgtgcc aaagatttgg aacagaattt
    14341 caaaggcata caaatcgtct gcttcggaca tctgggcgac ggcagcctac actacaatac
    14401 ttttctgccg gatatactca gtaacgaggc atatcgttac gaagatgaca tcaacagcac
    14461 agtctatcgc aacgttcttg cctgcaacgg tacagttgcc gccgaacacg gcataggcat
    14521 catcaaaaaa cagtggctgg acaaagtacg cacgcctgcc gaaatcgccc tgatgaaaag
    14581 catcaaacaa caccttgatc catataacat tatgaatccg ggcaaactgc ttccgtaacc
    14641 ggcatttctg atttgcatac acaacaaaga aagggacaat agatccgatt gtcggtttag
    14701 cgcgagctcg tgagtgcggt aaaaaattgg tggaaatgac acgaaaaatg accgcatttt
    14761 taaaataaaa aaatcggcag tgaatttccc tgccgatttt attttgttac aacttaactt
    14821 aaaacgtcca ctgcaaattc aacgcacctt gtttagtttg atgatgtttg cctgtttggc
    14881 ggttgaatgt ggcttgtaag gttaagtgag atttgatttt cactgctaca cctaattggc
    14941 tctcaattgc cgtcttattg tttatcactc gacgctctcc gtccatttcc acaccgaaag
    15001 gtttgttgtg gtaaagcgcg ttcacagcgg cgaaaggttc aatagcgata tttttataga
    15061 gtgaaaattg agctttagct tgaacgccca ctcggctttg taattggcga gagccaagta
    15121 aattcacgtg ggcattttcg ctatcgctga attttccgtt tacccccaaa taagtcaatt
    15181 gcgcctgtgg ttgtaggtaa aaacgaacac ggttgccctt tttagtgaag tgttccgcca
    15241 ataaagcatt gtaacccgct tcaattgagg cggtaatacc ttttgaagta aaacgttctg
    15301 tagcatcttc agtgttaatg cggtggcgga agcgttgata ttgtacccag ctatccgcat
    15361 acgcacctgt ctgtttgtcc tgaagctgat gccaagtggc gtaaacgcct gcaccaaagc
    15421 ctttcacatt tcccgttgta agattgtctg tatctgggtt gcggaaagta ctgcgttgtt
    15481 ctgcttgtcc gcccattaag ccaacggaaa gttgatagtt gtgattttgc aatgagaaaa
    15541 catcgccacc aagttgtatg cctttacgat ttccttctac tggtgcggtt ttaccttgta
    15601 catcttggct taagtgacca ctaattaaac gcaaccacaa gcctttgcgt ggtaaagtgc
    15661 ggtcaaaaat atcgctgttt ttgtcgttca aacgtaggtt gaataaagta ttggcagctt
    15721 gagcctgttg tgcataaatc gcaatatcat cgcgttcttg cactttggta aaaaagccct
    15781 ctgggcgttg ttgtaaagag agtgtataaa ttcccttttg gtgtttgcca gaaagacgga
    15841 atgcgcgttt atctgctgtg ccatttactt tgataatttg atgcccgtcg aggcttttta
    15901 aatcgtctat tggattttcg aagatgatgt tggaagtgcc agtaacattt ttctcaaaaa
    15961 ttaatgcagt atttttcgct tctttaggat cgtaagcaaa acgaaaacga gctccgccag
    16021 cataatcttc tttgacgagt aaactttcac ttttagtatt aaaacggatg tctgcattcg
    16081 ttgtttttaa ttccccaaca tcagaatccc aacggggttc ccagagagaa ttttctaata
    16141 tgaattgatc caaactaatt gtttgtccga taacgtgtga attgtctgta accgtaatat
    16201 agtggaatgg atctaaacca gaatatagat gctcttccca accagcaagc ttattaagtg
    16261 ttgggcgaag ccattcgata taattaccta cagataaaat ttcgctgttg atatggctat
    16321 tttttatttg tggagctaag gcgaaaatat cgctctttat taatcttggg ccagagtttc
    16381 cgaaggatgg ttgttcacac tgatcattat tggaattccc agtatttaca cagtccttaa
    16441 aatattgctg gagtgttttt tcttcttccc catttctttt ttcaaccccg aaacctcggg
    16501 cgaagccaac aaggtaatct tcagtgtatt ccttgtcata aaaagaaatc ttttttgagt
    16561 tgttgatgtt ttcgaattgg tgtgttttag agtatagtgc gggaactttg agagagttct
    16621 gagagctacc attcgtcggt tttagaagat aagtttcttg tttccagtat tcgctgcttt
    16681 tatctctaag tttttttaag cgatcaattt gctcatttgt taatttagaa ttgtcgtaaa
    16741 cgtaatcaac agccaaaagc ggagaggtat aaagaataga aaaaaataga cttataataa
    16801 atgatttttt aaacttctgc ttgcttgctt gcttgcttcg agtttcataa taaattttcc
    16861 tttgtcaagt aaaaataaat ggggcgtgga ttttagcata gaattgaaca aaaaatgtca
    16921 tttagctcac atttttctct atttatttct tatttattaa aagtaaacgt ttgctttttg
    16981 ctattttgtc aagccagttt gaaaatgtgt ataattgccc tcgttattta caaaaatttc
    17041 aggaaaaatg accgcacttt acccttggct aatgccaatt tatcatcaaa ttgctcaaac
    17101 ctttgacgaa ggtttggggc atcatgccgt actgattaaa gcggatgctg gtttaggtgt
    17161 agaacgttta cacatcaggc ggcagccttg cccataccgt ctgaagcact gtttccacaa
    17221 tcagcgcgta tgcttaatca accgctgttt ctcgcgtttc caatccgcct ctttcatact
    17281 ctgacgtttg tcgtgctgtt tcttaccttt tgccaaaccg atttccatct tgatttttcc
    17341 gcgcgaaaaa tgcaaatcca gcggcacgat ggtgtagccg gcacgttcgg ttttgccgat
    17401 taatttgttg atttccgact ggttcaacaa gagcttgcgc ggacgtacgg catctggttt
    17461 aatgtgtgtc gaggctgtgg gcaaagccgt aatatggcag ccgaccagat aaaacgcgtc
    17521 ttttttccaa tagatataac tctctttaag ctgtacgcgc gcggcgcgga ttgctttgac
    17581 ttcccagcct tccaagacca aaccggcttc aatccggtct tcaatgaaaa aatcgtgaaa
    17641 tgctttttta ttgttcgcaa tagccataaa catcctatca atatccgccg tcagacggca
    17701 taaacccgaa aacagaaccc atcataccgc ctcttcaacc gcctgcacaa tcttctcggg
    17761 atacagcctg ttgaggcagt cggtatgccc caacggacat tcacgtttga aacagggcga
    17821 acattccaag tgtaggctga cgattttcgc cctgtcgctc aaaggcggcg tatgcgtcgg
    17881 gctggaagaa ccgtaaaccg ccaccacctt cctgcccaat gccgccgcca agtgcatcag
    17941 cccgctgtcg ttgcacacga ccgtgtccgc caacgacagc aaatccattg cctgcgacaa
    18001 atcggttttg ccgcacaaat tgacacacat accgtctgaa aggcagttga tttcctcggc
    18061 aatttcatca tctttttgcg aaccgaacag ccaaacctgc caacccgccg ccgaataatg
    18121 tttgcccaac tcggcaaaat accttgtcgg ccaacgcttt gccggcccga attccgcacc
    18181 cggacaaaaa gccagaacag gctttccaat atccaagcca aaggtttcga cagaaatttc
    18241 ccgccgccgt tcatcaatgg aaaactcggg gaatcccgaa tgcccgtcaa aatcctcctg
    18301 gctcggatgc gcgagagccg tatatctgtc caccatcaaa ggcagacgtt ccttatccag
    18361 cctgcgtata tcgttcaaca gaaaataacg gctttcaccg acataacccg tccttttacc
    18421 gatacctgtc gccagcgcga tgattgccga tttcaaagaa ccgggcaaca cgataacctg
    18481 atcgtatccg cgccgcccca aatccctacc gacccgccaa cggcgtttca actccaacgc
    18541 accgtgtccg aacggatttt caagaatctc attcacttcc ggcatacgct caaacaccgc
    18601 catcgaccac ttcggtgcga acacatcaat cgtgcaaccg gggtgaagtt ccttcaaacg
    18661 gcggaacaag ggctgggtca tcacgcagtc gcctatccaa ctgggggaaa taatcaggat
    18721 tttgatggac ataacaagaa accgaaatca gacaggcgga attttaccgc gaaaccgttg
    18781 gaaaacctat cttgtctcat tccgaacgcc gtacgtgcaa atatgaaaaa gcccgaacat
    18841 tcaagttcgg gcttcaaaat tctggctccc cgacctgggc tcgaaccagg gacctgcgga
    18901 ttaacagtcc gtcgctctac cgactgagct atcggggaat ggggcgtatt atagcgtccg
    18961 gaaaaaatgt gtcaatcctt aattttggaa aaatgggcga caaaacgaca agcatatgaa
    19021 tcagaaagac attaagaccg atgccttaaa aggattgccg ttgtatgaat ttccacagcc
    19081 gtcatcacac catatttaag cccgatgagc cgttctgccc tcccccgctt aaaacaatgc
    19141 cgtctgaact tcgccgcgtt ccaaagccag taaaaactgt ttgcggttca acccgcccgc
    19201 gtagccggtc agtttgccgt cgctgccgat gacgcggtgg cagggaatca ggatagatac
    19261 tttgttctgc ccgttggcgg cggcaacggc gcggacggct ttggggttgc ccaaacgctg
    19321 cgcctgctcc ttgtagctgc gcgtttcgcc gtaaggaatc gccaagagcg cgtcccatgc
    19381 ctgcttttga aactcggtgc caatctgctc caaaggcgtg gcaaaggttt tcagacgacc
    19441 cttgaagtat aagtccaatt cctgccgcaa aagttgcgtc cgctcatcct cccgaaacac
    19501 aaaccgtccg cgcaaggctt tttggacggc ggcaatttcc tgttccaaat gcttctgtcc
    19561 gacaaactcc agcagacaca atcccttatt tccgaacacc gccagcatct cgcccaaagg
    19621 cgtggcaatg gcggcacaca ccagctcgtt caagctgtcg ggataacgca cttccaacag
    19681 acggatggct cggcggatac ggacatattc ttcaggcgcg cagccgatat tgtcccaaaa
    19741 atcccgatcg aactgcccgg cttcgcattc cgtcagattg ggatgcggca taacgccgca
    19801 ctcaaaaacc cgggtttcga ggcaatggcg gatttcatcc cattttgacg gcagattgtt
    19861 taaggaaggc agggtaatca ttcgtttgct ccgtatctct atcatggatt tggcggcaaa
    19921 atccccaatt ctcgtcattc ccgcacgccg atgcaggaac gggctatgac gtaaatcttg
    19981 agggtcgggt tgcggcaatg cctaaatatg cgatatttcc aaagcctcag ggaaaggaat
    20041 taaagacttc agacgacctg caaaggctcg tctgaaacca cgatttttgc atttgcgcat
    20101 tctggcacat catccaaccg tttcggcaca ttcctgccgc cgttgacagc ctataatgaa
    20161 tccacttatt catcaagcaa aggaatcatc tatgcaaacc cgcatcctct ccgccgtact
    20221 gctggctttt tcaaccgctg cctttgccgg gggcgcattc acgctgcaat tcgacaaccc
    20281 gtccgaagac ggcggcttca cgcaaaacca gcttttgagc gcgccttacg gatttggctg
    20341 ttcgggcggc aatgcttcgc ccgcgctgtc gtggaaaaat ccgcccgccg ggacaaaaag
    20401 tttcgtcctg accgtttacg ataaagacgc gccgaccgga ctgggctgga tgcaccgggt
    20461 ggtcgccgac attcccgccg atgtccaccg ccgcaacgcg acctcgctgc aattaagccg
    20521 ctgcgccaac atcgccgacc ggactgggct ggatgcactg ggtggtcgcc gacattcccg
    20581 ccgatgtccg ccgccgcaac gcggcctcgc tgcaattaag ccgctgcgcc aacatcgccg
    20641 acgaccagtc cgcagccata tcggcggtaa tcagtttgcg gatttgccgc atcaggttga
    20701 cgccttcgta cacggcaaaa ccgatgccgt catgctgcaa ccacgccaac acgccgcaaa
    20761 gcgcggcctc cgccgcattg tgcggcactt cttcatccgt cagcaccgcc gccgcataat
    20821 caaacgccgc gcccatccgc ccggggtgcg gcagcttcac cgcatcgcac actgcctgcg
    20881 ccgtcccgta ttgtgcggcg aacctttcta cggtttcctg ttcaaaagca atccattgcg
    20941 cctgatagag gccgtctgaa tcgggaatat taataacgtc aaacgtctgt ccgcctgcca
    21001 acgcaaccgc ctcacccgcc gcaatcctca attcgcgcct cgcccaccgc agcttcttgc
    21061 ttccgcgccg cacgataagc acagctggtt catataccgc cacgctgcgg tacaaggcgg
    21121 tatgatgttg cacgatgccg cccaaagcac ccaatcgttc gcgcgtatga aagtaatttg
    21181 acggcactat tatctccaac acgcagggcc gcacataagg cgcagccctg tttaccctga
    21241 tttggaaggg gttacttccc aaaaataaac caaaatccca ctgccccgaa tggccgggtt
    21301 tcaaccatat tccgtcggtg tgcctgcaac ttgactccct cccctgtggg ggagggttgg
    21361 ggagagggta aaacggggca gatacagaca atatttccgt taccgccccg atgccctctc
    21421 cctatccctc tcccacgaaa gagggaatgg attgccgttt aaataaaaaa atctacataa
    21481 aaatcaatg
//
LOCUS       DAWKOD010000017        21484 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000017 DAWKOD010000000
VERSION     DAWKOD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.017"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(112..2085)
                     /locus_tag="WJK73_000465"
     CDS             complement(112..2085)
                     /locus_tag="WJK73_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ7556995.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTKTAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSLVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRDHTHIAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2096..2656)
                     /locus_tag="WJK73_000466"
     CDS             complement(2096..2656)
                     /locus_tag="WJK73_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7556996.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVVESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2653..3171)
                     /locus_tag="WJK73_000467"
     CDS             complement(2653..3171)
                     /locus_tag="WJK73_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7556997.1"
                     /translation="MPAIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLGTVDIA
                     ITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGIDLDDAK
                     RRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVNRIEWF
                     KAGAINVTEMPS"
     gene            complement(3122..3355)
                     /locus_tag="WJK73_000468"
     CDS             complement(3122..3355)
                     /locus_tag="WJK73_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ7556998.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            complement(3352..4497)
                     /locus_tag="WJK73_000469"
     CDS             complement(3352..4497)
                     /locus_tag="WJK73_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7556999.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(4487..5818)
                     /locus_tag="WJK73_000470"
     CDS             complement(4487..5818)
                     /locus_tag="WJK73_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7557000.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(5818..7989)
                     /locus_tag="WJK73_000471"
     CDS             complement(5818..7989)
                     /locus_tag="WJK73_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ7557001.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            8144..8726
                     /locus_tag="WJK73_000472"
                     /pseudo
     CDS             8144..8726
                     /locus_tag="WJK73_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8770..9039)
                     /locus_tag="WJK73_000473"
     CDS             complement(8770..9039)
                     /locus_tag="WJK73_000473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557002.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(8979..9344)
                     /locus_tag="WJK73_000474"
     CDS             complement(8979..9344)
                     /locus_tag="WJK73_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557003.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(9348..9725)
                     /locus_tag="WJK73_000475"
     CDS             complement(9348..9725)
                     /locus_tag="WJK73_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7557004.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(9790..11199)
                     /locus_tag="WJK73_000476"
     CDS             complement(9790..11199)
                     /locus_tag="WJK73_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ7557005.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(11202..11399)
                     /locus_tag="WJK73_000477"
     CDS             complement(11202..11399)
                     /locus_tag="WJK73_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7557006.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(11396..12046)
                     /locus_tag="WJK73_000478"
     CDS             complement(11396..12046)
                     /locus_tag="WJK73_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7557007.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(12036..12458)
                     /locus_tag="WJK73_000479"
     CDS             complement(12036..12458)
                     /locus_tag="WJK73_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7557008.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(12458..12856)
                     /locus_tag="WJK73_000480"
     CDS             complement(12458..12856)
                     /locus_tag="WJK73_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557009.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(12921..13829)
                     /locus_tag="WJK73_000481"
     CDS             complement(12921..13829)
                     /locus_tag="WJK73_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7557010.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(13829..14839)
                     /locus_tag="WJK73_000482"
     CDS             complement(13829..14839)
                     /locus_tag="WJK73_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557011.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTSGKLLPAQKEWAEGVLKQPGGL
                     AFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESE
                     GK"
     gene            complement(15070..15486)
                     /locus_tag="WJK73_000483"
     CDS             complement(15070..15486)
                     /locus_tag="WJK73_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7557012.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(15614..16939)
                     /locus_tag="WJK73_000484"
     CDS             complement(15614..16939)
                     /locus_tag="WJK73_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ7557013.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(16926..18494)
                     /locus_tag="WJK73_000485"
     CDS             complement(16926..18494)
                     /locus_tag="WJK73_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7557014.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSLQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(18494..20116)
                     /gene="terL"
                     /locus_tag="WJK73_000486"
     CDS             complement(18494..20116)
                     /gene="terL"
                     /locus_tag="WJK73_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7557015.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            complement(20137..20643)
                     /locus_tag="WJK73_000487"
     CDS             complement(20137..20643)
                     /locus_tag="WJK73_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7557016.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            complement(20645..20842)
                     /locus_tag="WJK73_000488"
     CDS             complement(20645..20842)
                     /locus_tag="WJK73_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557017.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            complement(20839..21105)
                     /locus_tag="WJK73_000489"
     CDS             complement(20839..21105)
                     /locus_tag="WJK73_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557018.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            complement(21189..21445)
                     /locus_tag="WJK73_000490"
                     /pseudo
     CDS             complement(21189..21445)
                     /locus_tag="WJK73_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4352 a         6122 c         5582 g         5428 t
ORIGIN      
        1 atctctacag ccatctctac agctatctct acagctatct ctacagccat ctctacagcc
       61 atctctacag ccatctctac aaccatctct acaacccatc tctacagccc gctagtcttg
      121 cagtgcctcg agcaagccgt ctcggcggcg gcggtttcgg ggtccggttg atgttcttcc
      181 actctctctt tgcggttctt cgttggtttc aggtatttcg gggcctatcg gggagctggc
      241 ggcgtttccc gaggcggctt tgccgatggc catccagtcg caactgcctt cgtaccagtt
      301 ggtaataatg gcggcggcgt ggttttttgc gcggataaac agccagttgt gttgccttcg
      361 atcgccggta acgcgtcccg aatggcgttc ggatacgaag catttgacgt tgccgtcggc
      421 aaaggcgacg gggaagacga cttcgttttc gatggggccg ccgcttcttg taaacctgta
      481 actgccggtc tgtatcattg tgccgtcggg gaatctgaca atatccacgc cgccgatggt
      541 ttggcgtgtg aattgcgcgg caacggcggt gctgattttt tcgtccagcc cttggatttg
      601 ggcggcgatg tgggtgtggt cgcggtcggc tttgtcttgg aggttttgcg ccagcgtgcc
      661 cgcgtccagc gcgcctgcgt tgatggtttc gccgtgggac tttatccaga acacggcttc
      721 gcccaaggtg tcggcggctt tgatgcacag ttttaaaacc agggctttgg ggcggttttc
      781 gtcgccgccc gttgccattt tgctgtccgg gcgcggggtt aaaaagccgt tgtcgcttaa
      841 attttcgtcc gtccaagtcg atacgagcga gcttctctgc ctttcgttgt ggtcttcgta
      901 accgagggct gccgcgtgcg ggtggtttac ccagtgtgaa aatactttgt ggacgtgccg
      961 tttgatttcg tcttcctgct tcgttccgac tgccaagctg ttgcctgcgt tgcggataaa
     1021 gcggtcttcc gcctgcggga cgttttggat gctgccgtat tttccggtca gcaggcggta
     1081 cagctcggga taagcggttt tggttacgcg cgtgcggatg tcgtcaaacg ccagccagcc
     1141 ggtcgggatt tggtcggacg gaaaccacgc ggtgatgccg atgtcggtac ggcttaaatc
     1201 gggcaggcgg ttgctgttgc ccaaggcgcg gtaaaggtcg gggaaggtgt tttgcgcaaa
     1261 ggttgtgccg tctgcccgca aatagcctgc ggggtttcgg acggctttgg gaaaggatac
     1321 gatcgcgccg acggggatgc cgtctgaatt gagttttttc catgcggacc agttgtcgtt
     1381 tgcgctgctc gtttggtggc gttcgtacac gtcggacgtg taggcgggat aacccaactg
     1441 cctgcaccag ccgtgtttcg tgccggcgat gacttggata tggcaggcgg tgttttcaac
     1501 gggcaggttg gagctgccgc ccgccgtcgg cagggaatag atgccgtctg ttttgagggt
     1561 gttcaaatcg ccccggaatg tttcgacttt gaaattgccg atgccgtatc cggacagtgt
     1621 gttcggtttg ccttcgatgt cttcggcaaa acgcggtttg ttggctttgt ggaagatgtc
     1681 gtggttgttg taggaaatgc ggttgtttgc gccgtgcagg cggagggtgt tgtcgccgaa
     1741 gacgatgtgg gcgttgtcgg catccgcgcc gatgtaggct ttgtggccgc cgttccagtt
     1801 cgtctggttg gcggataaat ggatgccgct tgggaactgg gtttgggcgg taaaggtttt
     1861 ttggccgccg atttcttggt tgccggtggt aactacagcc agtctattca gtgctgccag
     1921 taattgatta ggctgcgact tatccggctg gataccggcc gcagtcagca cggccagcag
     1981 ctcggattgc acttggttga gccaccacgc gggcaggatg gtgcccaatt cggacacgcc
     2041 gtcgccgtcg tggaaggtct tgtcgggggt ttcgatgggg tgcatggttt agtccttatc
     2101 ggtaggtaaa tcggatagcg gtgtgggcgg gcttgaggcg gttgaacagg ctctcgatca
     2161 cggcatcgct gtaatcggtc agcctgtcgc ccgccgccga gataccggcg cggaatcggg
     2221 taatgcggtt gttgccgccg cgcacgttaa cgtgccacac ccacatgatt tcctgcggcg
     2281 caagacggtc gcccgcacgg ttgacaccgg cgcggaacgg ctgcggttcg tcgatttgga
     2341 tttgatagcc cgccgcctcg gccaaacgca caaaataagg aatactcaag ccgcctgttt
     2401 cgtttagctt ggccatgacg gccaacacac ggcgctggcg gtttttgccc gtaccgtcca
     2461 aacctaatac gcgctcccaa tcggccagca tttggccggc gctgcgcggg tcgacggcat
     2521 cggcaacgct ttgagccgat tccactaccg catccagcgc tgcgccgtct atttctgcct
     2581 gcgcccgcac acgcggggca ttgcgggcat acgacacggg gggcaacaga ccccgcaaga
     2641 tgtcttgata gctcatgacg gcatctccgt tacattaatc gcgcccgctt taaaccactc
     2701 gatgcggtta accgtatcgg cggcacggtt ggccgtcggc gcagtcagac ggcggtcgat
     2761 cacaccatcc acattgctga tagccgcctc gatttgcgac acagtcaggc cgtcgccggg
     2821 gatcagggtg tcgaaatatt cttttagagc cgtccgtatg cggcgcttgg catcgtccaa
     2881 gtcgatgccg tcgagcttga cttgcacggt aacaggcacc gccgttacgg ttggcttgag
     2941 taccagcgca ttttttgccg ttaccgggcg catctcgtcg atataagcct gtacgcggcg
     3001 cacagtttct tccgacggca caccgtcggc ggaggtaatg gcaatatcca ccgtacccaa
     3061 gccgcggcgc agcggataaa catatgcgct ggttacgccg tcaacactca acgcccagtt
     3121 tttatagtcg taacggttgc cgcctgcggg cggtcggcgg atggctggca aagccgtatt
     3181 cctgcaaatg ttcaaggtct tgcagggttt cgtcggccaa gccgctcaac tgcacgcgct
     3241 gtatcggctc ggacgacacc accagcgtga tttttccccg aaaggccgcg cgcagggttt
     3301 cgccgatatt tttagcagtt tgtgccgttt ttttcgccaa tttactcaaa ctcattcaaa
     3361 caccgccgtt tctgcttgtt ttttgccgcc ttttttgccc ttatggctca cgcctttgcg
     3421 tttgcctttg cgcttgcgcg ccgcctcggc ctttttgggg taagcgtcgg gtgtccaaat
     3481 accgtcctct ttgagccgca gctcggtttg cgtgccatcc atgcgggata gcataaaccg
     3541 ccgccccatc agaaaaaaca ccgcatcgat accgtgctcg tcgtcgatca catgcacacg
     3601 ctggccaggt tgccacaata cgccgtcgcg ggttttatgg ccgcccacgg ttatcgtgag
     3661 tgtcaatccc tccagccgcc agtcggccag ctgcttttta gcctgctttt gcaatgcggc
     3721 caaattgtcg gcatcggaca ccaccaccgt tttagggcgg tgcagcgtca tcgtcgggtc
     3781 tttgtacacc cactttaaat cgtgtttggc gctgtcgccg ctgcggccgt gcgattgcgc
     3841 caaaaaagta acctcggaaa agcggttgtc ggtatcccac tcaatgtcca tgcgctcgat
     3901 attgcggcgg ctgtcggtgc ggctccaaca caatgtcgcc accggcgggc tgctgtaatc
     3961 cgcaccgccc accaccaacg tgccgtccgg ctccagccac ggatgcagcc cgaccgagtt
     4021 ggcaatatgg gttaatgcct gccatacggt ttcgcccggc tcgatgtcga ttttgtccaa
     4081 agcggggttg ttttcggcct taagcaccac cgctttaatc tgcggccacg gcgcggccag
     4141 ctttttggct gcatccaata ccgtcatgcc ctttacattg agctgcggcg cggagcaatc
     4201 caccaaaaaa ccggccaaat cacgcccgct caagctcaac tcgcggccgc ccttgctttt
     4261 gccgtggcgc tggctgccga tgatgcccgt catcacgatt tgcccgtcta tcactacctc
     4321 gcagctctct ccgcttaaat cgggtatggc cgcctccggc cccaacctgc cgatgacaaa
     4381 atcgaagctg tcggcaggga ttaaaaagtc gctgtcgatg tcgtagcgct cccagtggcg
     4441 gtgctcttta ccgcccacgc gcaccgacac ggcatagccg tatgaattat tttgcatagc
     4501 tgttgaccaa agtgccgcgc ttgataaacg cggggtggtg gatatgggga ttgagccgca
     4561 ccagctctgc tgcgcgggct atatcgccgt aaaactcgtg ggcgatttgg tgtatcgtac
     4621 cgtcgattgg ggcttggcgc acaatcagcg gcggcttttg gttgatgacc gccgcaacca
     4681 acgcattcag acggcctgcc gccgcgcgca gggattctgc cgtttggtaa gcctcggtat
     4741 acacggcgtt ggccgtcagc ccaccagact cggcagcagc cgtctgcacc gcccgtaagg
     4801 cggcaatctc ggcctgcata cggcggcgca tggcgcggtt aacctcaatc aaatcgggcg
     4861 cggtcatctc ttcgccatgc gcctcgatta atgccgttgc caccgacaac agcgatgacg
     4921 tggataacag gcgcaccgcc tgcgctaccg gctgcacctg tttggcggtt aaacggttca
     4981 ggcggttttg caggccgtct gaaaagcggc cggtcagcaa attatcaggg atagcggcgg
     5041 cacggtcggc aacagccgca gccccgtcaa accgctgtcg cggcgaccaa ccggcatggt
     5101 gcatggcatt atcggccaaa cccgcctcac ggcgtatgcc agtatctacc atgaccgata
     5161 tatccgcaaa cagcttggcc gagccgtttt taaacgccgc tgcgctgtat ccgccccgat
     5221 cgggaaaggc aattttgtcc aaatcaaaca aacggcgcac cgcaccaaac gtacccgatg
     5281 ccgcactcca aatgcccagc gcgctgcccc ataaagccga tacgcccgca tccaccgcca
     5341 acaccgcatc aacaaagccg atagccgcct cgcggtaggt gtcgatatca gcaatcaacg
     5401 cctcaagctc gaccaaaaag gcgttttcaa aaacaaagat ttcctgcgct tcggccgcct
     5461 cgcggaaagt aatatcgatg cccgcataat ccacataatc ggcctcatgt cggtaactcc
     5521 atgatgccgc aatcatgttg tgcatccgcc cccaaacagg gtgcaccagc acgccgccgc
     5581 ccggctgctc cagcgcatcc agcagctttt tcagacggcc tgcatagccc ttgccccaaa
     5641 acaccgcatt aatctgcacc tgccgcccgg tcatgcccat gtcttcaagg tcgataccct
     5701 gcacaaacgg ccgcgcatgc tcggccaatg ccttgccgtt gctctcgtcc accacctcaa
     5761 tatcaaagcc gacgcccttg taagatgcgt cctgcaataa ggtatgccat ccgctcatta
     5821 ttgacccgct ccacggccga acatggccac ttgataccgc gatacctcat tcgcgataac
     5881 acgtccgtcg atattcaggc tcacattggt attgatggtc tgacttgccg acgccatcgc
     5941 actttgcact tgtgccaaac ctgttgtaac tgcagccgta tcctgcgcca atgctgcctg
     6001 atacgccgcc gtctgctgct gaatggctgc ctgatacgcc gccgtctgag ttgaaaacaa
     6061 cggagacaac atcttatcgg taggctgcgc tgacggagca gccaaatact gagggacacc
     6121 accccgcgcc tgttcccgct tcggcgcagg ttcaaatcgg ataccgttgt ttttcagacg
     6181 attaatcatt tcaggagttg tcttggtacc acgcaaaccc tttggcaatg tgccgtcacc
     6241 caaagactcg gaatacagca acatacctaa caaacccgcc cgtttaacta acgctggatt
     6301 attcatgagc ccgctaccgg cagattttcc ccatgacagc aacttaccgc ctgccgctgt
     6361 ggcacctgct gccacgccgc ctgcggaagc cttaccccat accaacgctt tactacctac
     6421 atctttcaga aagcctgcac ctttaccgcc accggcggtt aataacattg cggcagacgc
     6481 cgctgttgcc gccgtcgtca atgcttgcag ggctagagtg gcattagggt attccgctgc
     6541 cagcttttta aacgaggttt ccatatccac caatgttccc gtcagacttt cccgcaacat
     6601 tgccagcgat tcctgttgct cttgttgcgc cgctgccgac aacattcgcg cctcgttgtt
     6661 cttagcaatt tttccgttat ccaacgttac gccagccaac gaagccatat attcacggat
     6721 ttgcgtcata tccgttgcag ccagcaaacc ttgttttgcc tgcaaatcag gcagcagttg
     6781 cgccaaaagc gcgcccttaa gcatatttgc ctgctccgcc gccgtcttat cgcctgcagc
     6841 cgcgcgtttc ttataatctt ggtattgctt atcctttact agcatggcat cggcaagacg
     6901 ggacaacacc tgcactgcgt tttcgccgtt ttgcttgcct tgcacaaccg agcctatcca
     6961 atcgacacct ttcttcgggt catttggatt tgccatcttc ttcagacgac ctatcgtgta
     7021 aggcgacaga gttttactca aaagattttg cacattagtc gccgcttcgg caggactgcc
     7081 cgatttattc gccgcagatt gtaagagtga gagcaggtag tccaaaccgc cgacaccatt
     7141 catccctgcc tgttgcgcgg cagggagcag actcggcagc tcccgaacca tatcccgcac
     7201 ctcgaaagtg ccgtctaaac ccgattgcaa gacgtgctca agcccgagtt gcaggtcttt
     7261 accgctcatg ccgccatctt tcagggtttt aatcagtttt gccgtatcct cgccactgcc
     7321 ttctgaggca agtgcaaaag catatgccgc ctgcgcttca ttcttggttt gggcaaaatt
     7381 cagactggtg gtcatcatgc cgctgattaa atccaaagcc ttatcgtggg tcccgccatt
     7441 tttctcgaca agttcaagtg ccaaatcctt gatctgttgc gcaccttcag ttgcaatcca
     7501 cgctgccgat ttactgttat cctcaataaa tgcctgtctg gacacgcggt tgatgttctc
     7561 atcaagctgc tttctgttgt ccatagcagg cttaagcacc gtatacgctg ccgcgccacc
     7621 agccaccgca gctgcaccga atcttccgat ttttcccatc ttgtccgcaa atcccgtgcc
     7681 ctgtttcagt tccgcgttca gctcgcgaat tcggttacgc gtagcgaccg ccgcccgtgc
     7741 caaatcattt tgtgatgcct tgccgctgcg cgccaaacgg ttaaacgcag cctgtgtcag
     7801 catgatttcg cgttgaatct gtttttctga gcggatgccg gtcaacgcat aagtatggct
     7861 ttgccgttca agttgcgccc gcgatttcgc ggtacgatct aattgtcgtt gagtatcagc
     7921 cagtagccgt ctcgctccgt catctcgggc ggttaacacc aacgacagtt tcatgttccc
     7981 gtttgccata aaaagcagcc tttaaacgta ttaactcaag gtcaattatc gtttaaaggc
     8041 tgtttaaggg aggcggtatg ggcttcaacc ccgttttcgt gctttatttg tttgttgcgt
     8101 tataatacca gaatgataac cattttattc aaagggatat tttatgaata tgaaaaaatt
     8161 gatttccgca atttgtgttt caattgtttt atcagcctgc aaccaacaat caaaaacggc
     8221 acaagccgaa gaacctgtcc aaagtatcca ggctgctgat tgtaccgccc caatggacat
     8281 cacagttgaa caatatctca tcaatttgga gcaagcattt aaaactcaga acgtctcaac
     8341 aaaaatccat aataaaaata ttgtcaagac cgattgtggt tatgacctta ctttggtaat
     8401 ggattttggg gcgattgcgc tcaaactgga cgagcagcaa aaaattagag ctatctcagt
     8461 aggctacatt ttaaaaaccg acggagagaa aggacaaaat ctagtcaata atgccataaa
     8521 tggattacac agtattcagg cagttctgtc tttaactacc acagacaaat tgggcgaatc
     8581 ggaagcagga aaacaacttc gtcaaagaat ccaatcagac aggagcaaca gcgcaaaaag
     8641 acgttccggc agatggtatt ttatatagcg ttgtttttga aaaagaaaca aacaccattg
     8701 caataatcgg cagaaaacaa ccctaaccgt caaataaaaa aaaggtcgtc tgaaactcag
     8761 acgacctttt tattttccct ccggctttct cagccgtttc gacacaatca ctccctcttc
     8821 cttcggcgtt tttatgccca ggctcgacag gatgtcttca aaccatgccg acagctcggc
     8881 gtggcacata tcgcgcactg cgtccgcccc tatccccgcc tttgccatca ggatgaccgc
     8941 ctgacggtag cttttgtgtg ccgtcaggta gctgcgtttt actgctccgc cgccgacggc
     9001 gcgtcggggc tcgccgtagc ggcggacggt tttaggcaca aatcctcctg agccgccatc
     9061 acggtatggt agtcttcggt agagaggttt tccatcagcc actgcgccgt caggatgtcg
     9121 gatgggatac cttgggcttc aatctgctgc gaccagtagg tcagcgtctc taggacgtcg
     9181 cgttgcagca gttcggcttt tgtggcgttt tcgggcagct cgggcaagtc atcaatcagg
     9241 gcgaacgccg ccagttcgcc gccgacggtc agtggcttga ggcgtaattc agatacgact
     9301 ttgccattgt aggtcagccc gtgggtcagt ttgacggtaa tcacgcatta ctccttgatt
     9361 ttgtgcaaag caatcatctc aatgtcgatg cgtgcctcgt tatcgacttc atattgctcg
     9421 ctggtatcga cggtaaagca gtcgaggtag gaagtgcggc gaccttcgtc gttgatgggg
     9481 taaatcgtga ttttcgcctt ggtgatgttg tcccagtcga tttccgtacc gtcgatcgga
     9541 atggcggcgg taacgcttaa tttgtgttcg gttacgccgt cacaataacc gttgactcgg
     9601 ccgttgcggt tcatcgtttt gaccggcttg cggcctgtag tggtttgcgg tttgatgctc
     9661 acaatctcga tatcgcggcc gttcacctcc ataatcaccg cgccggtata ggtagcgtcg
     9721 ctcatgcttt atcctttttt aaggtttcag acggccttta aacctgtttt aaacgccgtc
     9781 tgaaaagggt tacaaaatca aatcaatgcg cccggcaaag acgtgcaggc cgttgaccac
     9841 atcggcgggg ataatggcat taacacggtt ggggtcgttt tgcgcacgcg ccaccaccag
     9901 cttgcctttg ttggcctcgg cgttttcgat gatttcggct tggtcgagct taatcagcac
     9961 gtccaaaatc tcgctcttaa ccttgggcag caggcggtcg ctcaatttgt cgcgcggaaa
    10021 acgcagggca atgcgctctt taacgctgcg gcgcacataa tccagcgtgc gaatggtggt
    10081 aatgtcgagt agtgccgggt cgtcggtgtt gttggccgac ttggtatagg ttgatacggc
    10141 acgcataatc tgcacgcggt tgttgaccgc tgtgagcggg gtcaagccgt tgtacagcgc
    10201 attgttgcat tctgcaaacc acggccattg cgcatcaggt gtaacggcca gccctttgat
    10261 ttccagcgtg ttcagcggct tggcagggtc ttcttcaaag gccaacaccg ccgcataacc
    10321 tgcggcgatg atgccgtttg gctctaccgc acctttgtac caagcacagg taatgcggcc
    10381 gtcgttgatt ttgccggtag cggtggtggc cgtgctcaag gccgcactca tacccaatac
    10441 gccgatacag ccgcgctgct cgatggcgtt gaatacgttg gtaatatggt tgctcaaggc
    10501 tttggcgttg gcgtcatcgc taaacgggct gcaaataatg tgataatgct tgcccgccac
    10561 tttggacaag gccgtggcaa tgtccgcatt tttggcaccg ccggtaaaga cattggcttg
    10621 ataagtcagg ccggtattgc cggtgctcac ggttaagccg ctctcgttgc cgatctcgcc
    10681 tttgtgtttg gccgtcagcg taacggctgc gccgctgccg gatgcggtaa cggttacatc
    10741 ggcggcagtg atggcggttt tcagacggcc tgccactgtg gcggcggtct cgccggtgtt
    10801 aacggccgtg cttacctgct tgccgccaat cgtgatttcc accacgcccg gcgcggtggc
    10861 cgtgccggaa agggtaacgg ttgcggttgc ctgcacgcct gcgctgtggt cggcaatacc
    10921 gataacggtc aaatccaaat aagggttgtt ggcaaatgct tggcgcacca tcaaatgcgc
    10981 cagcgagcct tgtccgaaca aatcggccgc ctcggcatcg ctaaatagtt gcaccggctc
    11041 taaggcgggc tgtatgcccg cggtcagcat gggtgcaacc atcaatacct tttgcggatt
    11101 ttgcggcaaa ccttgcacgg cattgcgggt gttaaattca atatactgcc cgggcacgcg
    11161 gatgctgccc ggaatcgtgt caaaatcaat atggggcatc attactctcc cttagtattg
    11221 cgggttttgg tatttggggc ggcatcctca accaccacca aatcgccgtc atcaatcatg
    11281 cggcggtaat acaggctgtt gccgtccacc tccaccggct cttggccgat atattcgtgc
    11341 gggttgtgtt cggtcggcac acgcaggcca tctgccgccg ttactttgat tttgctcatt
    11401 gcttatcctt aagggttagg tttacaggta tttcatcggc ggattgcggg tcgtaaattt
    11461 tgccgtccaa cccctcgaaa tcaggccacg gctcgctcaa tgtaccctga tacttggtaa
    11521 agatatggtt ggggtcgtcg ggattgtcgg tgcgttcggg gtagcggtca ttttccaacc
    11581 cgcaggtgtt aaagcggatg gcatactcaa ccgcatatat gcttactgcg gcgttttgca
    11641 ccagcacatg attggcaatc gcgcgcaccg ctttgggcac caagccgcgg ctatcggcaa
    11701 aaccgagccg ctggccgtca agcaggcggc gaacagcgcg gattaaatcg ttgctgccga
    11761 tttcgcggct gtcgatgccg ccttgccgct gcgcctgctc gttgcgcaga ttgcgggccg
    11821 ccaccatcac cacaaattcg gcggtatcct gataacgtcc gcatacgccg ccggtgctgg
    11881 caggctcaac tttgctgccg ccatacgtta cccaaaccgc aggcagcgta tggatttgcc
    11941 ccgccaaatc gtcggcctcg ccgttgtagc ttttaaccgt gcgcaccatg cgacccaagc
    12001 cccgtttcag acggtctgtt atcgcttgct caatatcagt gatcacgccc gaacacctta
    12061 tttttaccgt ttacaaacac aataccgtct tcgcccgcgg ccacttcttc gccgctatca
    12121 tccacgccca gctgcacttc gcctttggcc atagcacgca gcaggtcgag cacgtcgatt
    12181 ttgtagcggt tgcggatttc gtcggtaatc aacacgccct gagccgccgt cagacggtag
    12241 cgggcaatgt cgcagcaaag gcgcaccaag atgggcggca gatcctcaaa aggtcgtctg
    12301 aaccgcccca gatacgcgtc gatttcggca gtggcgtcca ccagcgcggt ttgtgcgacc
    12361 tcgcggtcaa tcagcccctc gttgttgcgg tcggtgagct gcaagacttc cagctcaccg
    12421 aaacgcgcaa ccatatcctc aaccgtcgcg tatgccatta ctcgaccgcc ttgcgttgca
    12481 gcataggctc ggcgcagatt gccttccaca ccgcttcgcc gacttcggcg cgcttcactt
    12541 cgcgccagcc gccgtcaaac agcaggccgc cgcgccaaaa ttctttgccg tctgcgccgg
    12601 tactgacgag catcacatcg cggctgtccg ccaaagcgtc ggcggcgcgt tgcgtatgct
    12661 gcactttgag ttcggcaagt tcggcggaca gtgccttttt gtcgtcttcg gctttttcca
    12721 aggctgtggt cagcatttcg acatcgtttc gggcggcggc aagctctgcc tgcacggcgt
    12781 ccaattcggc tttgatgtct tcaaacgacg gggcggcggt ttcggcggtt tctggtttgt
    12841 tgttggtttt tgccatgatg actccttgtt tcagacggcc tttaaatagg tgttaaaggt
    12901 cgtctgaaaa cggttcgggg ttacaggtgg acgcaaacgt gcagtttcag acggcctttg
    12961 aaggtattgg tcgtgccgtt gattttgtcg gcttccaata attcacgggc tttgtcttcc
    13021 agctcgggcg gaacgaccaa gaggctgggc ttcacattca acacatagcc accgtcggct
    13081 ttgattttct gcattttgac gataatggcg gcgaggtttt cgcgggtcag cgcggttttt
    13141 tccgccatgt gcatcagttg ccacaaaccg aaaccggcat tgcagcggcg gcgcgatccg
    13201 tacaggtatt cgtcttccat aaacacgcgg tcggatttgg acgggtcgaa tttggtttca
    13261 aactcggagt cggtgcggtt ttggaaaatc agcggcttca gggtcttggt gtcatcgacg
    13321 acgtagaagg tcggcgcgtc gttgtccgta ccggtcgtca gattggagtg cggcgtattc
    13381 tgacccgtgc cgtccgattt ctcgtaaacg gggtggtcgg tatcgaagaa atattggccg
    13441 tcataaccga cagtggtttt accttttttg agcagacccc acaccagcgt atcgggcagg
    13501 gcggcggcgg actcgcccat tgccgccatc atcgggcggt acatgccgac ttggtcgtct
    13561 tcgatgtccg tacgcgctac gccgacggtt gcttcaaact ttttgttgtc gaggctcatc
    13621 gcctgcttgt tcattttttc gatttggcgc gagccgaccc attcgcgcat ttgggggaat
    13681 ttgccgagcc acgcataagt attggttgcc gtattggacg gaatggtcat cgcaacggcg
    13741 gtatagtcgg gcttgacgga ttccaaaccc gtttgaaatt ctttgcggaa tgctgccgtg
    13801 attgcggtca aaatcgctga tttgtccatt acttaccttc gctttctttg atttttacaa
    13861 attcttcgcc ggacatgccc agcatttttg ctgcggctgc ctcttccgca gtcagtgcgg
    13921 cgacgcgttc gtcgggtgct ttaccgcccg tttgcgagcc tgccagtgca gcgaccggct
    13981 gggcgttttc aataaagccg gtcaaaaatg ccaagccgcc cggctgtttc aatacgcctt
    14041 ctgcccactc cttctgagca ggcagcaatt tgcctgaagt cagcgcggcg actttgcttt
    14101 gcagctcttg aaccactgag ataggcgcgt atttagtcaa atcaggcttg gcggtttgcg
    14161 ccgtcaatgc cgcgatgcgg ctgtcttttt ccgccagctg cgcgaacacg tcggcagaca
    14221 atgccacgtc tttcggcttg gcttccacga gcgcggacaa tgccgccttc agttcttctt
    14281 cgcccgcatc aggcagaccg aacagttgct gtaacaactc tttcataggg ttttgctccg
    14341 tttccggttt taaaatttgc gccgacgctg ccgccagcac ctcgtccata ccgtccaacg
    14401 cggggaaatt tgtcagcgcg gcgtgaaaaa ttttgcttac atatcccttt gtgtcatagg
    14461 aaaacacagc agagatgtag cgatactctt ttgcggcaat tgccgcagcc gccttgtccg
    14521 tccactccac ttcggcaaac atgcctttag gcgtgaactc cagccaacgc atccaaccgg
    14581 cggcaggtgc aggttgtccg tttttctctt tgtagagcgt ctggtgttca taatcgacaa
    14641 ccaactgatt gcgcgagctg ttggccaaca acgcgacatc atgaccgttt tcttcggtca
    14701 gataccacgc agggacatca gtcggacgac cgtctactgc gcgaaattcg ccatatggca
    14761 gcagttggat tcgcccgtct tttggctgca cctcgaaact gcacacggca agtagggttt
    14821 tttgtgcatt tttggacatg tgtacctctc tcaatacggt cattttgcct atttgaaaaa
    14881 acaacaaaga gtaggcgtgc ttcaaaccct cccgccattt ttttaaaacc tgtccgcgcg
    14941 atacacaatt aatcgttttc aggggctttt tagaagcgtc taggattgat ttttatatct
    15001 tgggcagggg tttgtattat ccgcataaaa aaacgcgcta aaaagcgcgt ttttgagggt
    15061 tttataaatt cacggtatca gacccgaaaa ataatcctgc acatcgtcca tcaaagcctg
    15121 tttgtcgtcg tccgtcagcg tcaaaaattc ccgttgcgga atccgaactt tgcggttgcg
    15181 ccccgccata ccgccgaagt tgtggatggc ggcatagacg atattcgtac cgacaagggc
    15241 tgtatcgttg tctgacagtg tggaaaaact gtctttcaga cgacccgaat ccgaaagcgg
    15301 cttgccgtcg cggtatttta gccccaacca tttcggacgg cctgcgtagc ggaaattgag
    15361 cttgaccgcc gtgtgcatgg tttcggacag tcggcgcatc agcaggtagc ggttttcgat
    15421 cccgttgcca agccgctcga tttggtttag gacgacaaag atattgtcta ttttgacatc
    15481 aatcatggta ttatcctgcc taggctacta aaaaagcggt gaatttgtct gccctccgca
    15541 gcccttatgc agtcctcgga ctgggattgg tatccgttac ataagcgaga gtgtgtcggg
    15601 attgcagtcc gacttagtag ccttttttca tttcatagcg tttcttactt ttcacaattt
    15661 ctttttcatc cgccctgcga aacgaatcca aaaaaatcga atcgtactgt ggcagatatt
    15721 tcaaaactgc catcaatctg tatttttcgt tcccctccaa cggttttccg agcaaaacaa
    15781 aactttcttt tcctctgctt ttgtcataaa aaatatattc gggctcatga atcagatcgg
    15841 ggagcatggc atataaatct gttccaaacg attgcccctc ccgactgtcc acctgtttga
    15901 ccaatgtatc atcagacagc cacaccgtcg ctcgagtcat acctgccagt tcttgcattt
    15961 ccttgctcaa tacacccgca gcaaatttaa gctgtcttga tagcgcgttt cggattttga
    16021 ttttctgctc tttatcgggc ttgccatcaa tattcaaacg ttgcttgact tcataaaact
    16081 ctttttcaag ctgtttaaag ctggttttaa aatccgcacc acccatttcc gctttggcaa
    16141 attgatgcgc caacgcacgg tcgtacttgt ctaaatcggg gcggtagttc attcgtccgg
    16201 cgttgtaatc aaatcctcga tcggtcgtgt acagactgcc atccggtgct ttataggcaa
    16261 gggtcagata agtatcgcct ttcttattgt agattttatg ggtctcaaca agattgtccg
    16321 acgtgctttg cccgacaatc cgcccctggc gttccacatc ccgctccgac agcgcgatga
    16381 ccgagcaacg gcagttgtag ccgttgggcg ggtaaaacgt tgcccaaaac gggtcgtcgt
    16441 agcggtacac cagcccgtct attgccgaat gcgccggacg ggtgcggctg tcgcctaccg
    16501 cgtcatacat ccaataaggt cgtgcatcaa tatttgccat atatccttga tattgaccgg
    16561 cgttgtaggc agtttgcatg ttggtacggt aaatcgtctc caacctccgc ggcgaaccga
    16621 atacctcgcc ggtggctggg tcgatgatat ccttaccgtt atgcccgttc ggatgcagcc
    16681 agcccttgtt gctgagcaga ttcaagatac cttttcgcca atcgtcaaac gactgtccac
    16741 tttttgccga ttcaaccatc gaagttttga tgtcgttgag catatccaag tcggtcatgc
    16801 gggcaatcgt atagactttg gcaatttcgg aggctgtcag attgcggtag ctctccgccg
    16861 taaccttttt actttccagc cactcgattg ccttttttgg cggcagactt aaggcaaatc
    16921 ccaaatcagg cgcgggcatg gtcttgtccc aaaatatcgc tgataaacaa ggcctgctgc
    16981 atataggtac gcagtttcgc gttgtccaaa ttcggataaa gcgcattcag tgcggcatct
    17041 gcctcctcgt agctgttgca agcattaagt gcggcaaccg cctgacgcac catcgggttg
    17101 agctgagaat tgaaatcggg ctcaaccagc gcgtcatcca acgcgccgtc caatatttcc
    17161 tgatgcctgc ccgtagcctt agatggtacg gtgtgggcgg ataaagccgc caatgcagtt
    17221 ctgtttatcg gattgtccgg tacctgccgc accaacacag cctcaccctc ctgcacatct
    17281 ggaatgacca gtttgtcgcg cacccagctt tcggggattt gtacgccgac atccaccagt
    17341 ttcgggatag cgtcggcaaa gaccgcgatg tctttcggct cgcgcgtgtc aaattcaaat
    17401 ttcggcacgc ggtttgggtc ggcattggga tagttgattt gcaggaacgg tccgatgatt
    17461 tggcttgtga tggtttgcgc cacctgtttt gcgtccgaca ccagcaaatc gcggcgtacc
    17521 tcgttgtgga tattgcccag cgcgttggtg ctggattttc cgtccgcacc gctggttagc
    17581 gtttgcccca aaatcagccg cgccgccgat ttttcgcacc agtcggccat ctgcaaaaac
    17641 ggattgctgg ttgccatcgt accgcttgca gcgttgtgca gctcgatttc cataccttct
    17701 ggcatgatgc ctgccgcgtt gtgaccgatt tccgccaccg ctcgaagcag ggtgtttttt
    17761 tcctctttgg ttgcgcccgc gccgtatttg ccgatacgga tgggcatgcc gtacagctcc
    17821 aaaaactcgg caaaatcgtg gacggcgtag tgtttgaaca tatacagcca ggaaagcgtg
    17881 cggaaaagcc cgttgcgcgc ctgctggagg ctgcgcgatt tttgggtatg aacgacccag
    17941 cccagcggcc acaacgcttc gccttccgga ttttcgcggg tacgcagcag cagcccgttg
    18001 tctttgtccc atttgaacca gctttgcggg cggtggataa agtttcgggg taggtaaagg
    18061 ccgtctgaaa atacccactc gacctccaac gcagaaaatc cgtgccctac cgcgtccatc
    18121 aaatccataa tcaggtcttc gagggtaggc aggctgtcca tcatttcgta ggcttggtcg
    18181 gacagctttt cttcttcggg cgtcgcattt cgcggcgggg cgacgcgcca gttgagcgtc
    18241 agcagcgcgc gtttgcgcgt ccccatattt gccgcgatgt cgctgtcgcg ctcctcaatg
    18301 tccgcgaaaa gctcgtgttg ggcgcggatg tcgccgcttt ccgcatcctc gaagagagcc
    18361 cgcatctttt gcggtgtaat aaaattggac ggatgctcgg cgataacccg accggtcgcc
    18421 gtaatttgag ccacgtccgt ctgcaatgca gcttcgggct tttggatttt agttttattg
    18481 ttcttttttg ccattatcgg ctccatttac tttttatctc gtcatcaaaa tcgttatcgg
    18541 tagggctttg ccattctatc ggcacacaat tggccaccgc ccccgaccag agcatatgca
    18601 ctgcatcagg accatcatca tgatcagcct ttggaaaatg gcggaactgc tgtatcagcg
    18661 tctgttggct ctcattcaac agaatcaaac cgttcgccat gtgaggctgt aaagtctcaa
    18721 tccgcaagag cttgtccgat accggcttga ccgcccgcgc cgggacaggt attccacgcg
    18781 ccgcgctgcg cttgaccagc tcatctttca aaaattcctg aaactgaacc gtctcaacaa
    18841 accacagttt gcaacgatat tgacggtgca atctgataac gtcctcaata atcaaatcag
    18901 ggagacgttt tttaatctga gcttccacga catacagttt gccggtttca cgttgataac
    18961 cgccaatgat aatcgccgac gggtcacggc tcgcccccgc tttacccagc gacgggtcga
    19021 gcgcaccgaa atacaccaaa tcggacggca ggtcgttcca aaacttcatc gacttggcaa
    19081 acggcgcatc ttcgccgctg accgggtcgt tttgatattc gctgtcaaat gtcgcatgac
    19141 cgtcacgcgc acggattttc atcagcgcga gtacgccgcg agccgcccag cttgtttgcg
    19201 caccgcgctc catctcgtct ttattggcga gataaaacgc ctcggccaca gtctcgccgt
    19261 cgtttcggaa aagttcctcc catctgtccc acaaatccat gcggtcgggc cagcgtttca
    19321 tcgccttaaa cttaataccg tgccaaaacg ggttattcaa agtgcggtta agtacgctgt
    19381 cgtaatgcaa aatcgtgccg atatatatca catcgtattt ctggccgaca ccgcccaaag
    19441 gcaatacagt cttagtcagc cacgcattga gcttgtcgcg ctgttcgggg ttgcggactt
    19501 gctcgtcatt ctcaatatcg tccaaaacag ttaagtcagg acggtaaggg ccgtgacgca
    19561 aaccgcgcag ctttttaccg ctaccggcca cttggacttt aacgtcattg gccgtcacaa
    19621 tcgtaccggc ctgccatacg cggccctgcc cgcatacttc cggaaagtcg gttttcaagc
    19681 gcggattaaa ttccaattcc gccttgatgg cttcgagcat cgggtatgcc tgatctatgc
    19741 tgtccatcac aataacggca taatgttttt ggcctgtcac aatacaccac agcgtaaaca
    19801 actgagtaac ctgcgtcgac ttaccctcgc cacgcggcgc acccaccgcc tcattttccc
    19861 ctttggggga gcggataatc tccggcagac ggctgaataa aaacgcatgc agttcggatt
    19921 tttcagggga gcggatataa tgcggaaagt aggtgttgac gaaatactcg taaccgccta
    19981 ccgggtcaaa caccttggca cggcgtgcag caatagcctt gggcgacgca tcgaagccgt
    20041 ccacctctgc ctcaataatt tggcggagtt gggcggcata ttcggcaagt gactttaaaa
    20101 actctttgga cttcattcaa ttaccttatg cagttgctac ctgtatttct tttcaatttc
    20161 cacacccaac ggctcgacca gctcgacata agcctgcaag tgttgcgggt atcgctccct
    20221 gaccacttcg ccgaacaatt ccaacacctc gattgccgtc gccagttttg acgtttccgg
    20281 catcactttg gcgtttgccg ccacggtctt ggtaaacgcg tcggacaggc ttgtcaacag
    20341 tttggcgcgc tcggacggca tcagcccttc aatcgacgtg tcttgcaaca ttgtcatcgt
    20401 tgactggtac tgcaccaaaa aacccgccag cagcgaacgg ctcaagtctt cgatgccgcc
    20461 gcctgccaag gtataggcgg cgcgcacttt gtcccaatcg tcgctgcttt ccttggcggc
    20521 gcgtttccag ctgcgggcgg tcgcaatcgg gatttcgcac atcatcgccg cgatttcaag
    20581 cgtctgcccg tcgctgacgt acaatcggcg cagcttttcg cggatttcct tcggatgcgc
    20641 catatcagcc cccgaacttg gctctcagca gctcccagcc ggtcgttaca atcacaccgc
    20701 cgagaccgcc gtaaaccgcc gcagatttct tgcagtcttt cttaatttgc tgcaattcct
    20761 cgtccattcg agcctgattg gcgagcatgt catcctgctt ggcttcgata cgcgccaaga
    20821 cttccaaaat cgggtcgctc atgatttgtc cgctttcctg tccaattttt cgtttacttt
    20881 ttccaacttg ttttcaattc gttctaaggc tgccgcaata ttagtgctgt ctgccttggc
    20941 atcttgcttg gtgtgataag agagcttgac cgcgtgcagc tcctctttaa ggtcgtcaat
    21001 gcgcttgtcc gcctctttca gacggcctga aatgccgttg acccaaaacc aaaacgccgc
    21061 cgttgcaatc ggccacaggg ttctgaaacc aaattcaaag tccatttaaa acccctttaa
    21121 accggcacat cgccaaatac gatacggacc gcgtagcctt cgggatgacg actcgccgcc
    21181 tcgacctttt agccgtctga aaagactgag taatatttcc gcaaaatacc aatcacatca
    21241 gcaggggcgt cgcggaaaac tccacacaaa aggtcgtctg aaaatcctta tccatgcgta
    21301 ccgcgtaatc gataccggcc ttatccaaca aatcggaaac atgaatgaca aacggctctt
    21361 gctcgcgtgc gcggctcaat cccagctcta aatccgcatg gcggcaggcg accgtgcgtt
    21421 gcaccaactc acgataagtc gtcatcgcgc accctccgaa ccgtcaactg ccgcttgact
    21481 gttg
//
LOCUS       DAWKOD010000018        20880 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000018 DAWKOD010000000
VERSION     DAWKOD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.018"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJK73_000491"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJK73_000491"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ7557019.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJK73_000492"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJK73_000492"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ7557020.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3196..3897)
                     /locus_tag="WJK73_000493"
     CDS             complement(3196..3897)
                     /locus_tag="WJK73_000493"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ7557021.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4083..5195
                     /locus_tag="WJK73_000494"
     CDS             4083..5195
                     /locus_tag="WJK73_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7557022.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLLDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5229..6206
                     /locus_tag="WJK73_000495"
     CDS             5229..6206
                     /locus_tag="WJK73_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ7557023.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6210..6560
                     /locus_tag="WJK73_000496"
     CDS             6210..6560
                     /locus_tag="WJK73_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ7557024.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6565..7344
                     /locus_tag="WJK73_000497"
     CDS             6565..7344
                     /locus_tag="WJK73_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7557025.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            7370..7585
                     /locus_tag="WJK73_000498"
     CDS             7370..7585
                     /locus_tag="WJK73_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ7557026.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            7686..7997
                     /gene="grxD"
                     /locus_tag="WJK73_000499"
     CDS             7686..7997
                     /gene="grxD"
                     /locus_tag="WJK73_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ7557027.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8103..8729
                     /gene="upp"
                     /locus_tag="WJK73_000500"
     CDS             8103..8729
                     /gene="upp"
                     /locus_tag="WJK73_000500"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ7557028.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8752..9072
                     /locus_tag="WJK73_000501"
     CDS             8752..9072
                     /locus_tag="WJK73_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ7557029.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            9323..9745
                     /locus_tag="WJK73_000502"
     CDS             9323..9745
                     /locus_tag="WJK73_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557030.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            9763..10524
                     /locus_tag="WJK73_000503"
     CDS             9763..10524
                     /locus_tag="WJK73_000503"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ7557031.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            10539..11873
                     /locus_tag="WJK73_000504"
     CDS             10539..11873
                     /locus_tag="WJK73_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7557032.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            11870..13087
                     /locus_tag="WJK73_000505"
     CDS             11870..13087
                     /locus_tag="WJK73_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ7557033.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            13162..14226
                     /locus_tag="WJK73_000506"
     CDS             13162..14226
                     /locus_tag="WJK73_000506"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ7557034.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            14415..15794
                     /gene="radA"
                     /locus_tag="WJK73_000507"
     CDS             14415..15794
                     /gene="radA"
                     /locus_tag="WJK73_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ7557035.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(16070..16549)
                     /locus_tag="WJK73_000508"
     CDS             complement(16070..16549)
                     /locus_tag="WJK73_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ7557036.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(16791..17150)
                     /locus_tag="WJK73_000509"
     CDS             complement(16791..17150)
                     /locus_tag="WJK73_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ7557037.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(17189..20803)
                     /gene="recB"
                     /locus_tag="WJK73_000510"
     CDS             complement(17189..20803)
                     /gene="recB"
                     /locus_tag="WJK73_000510"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ7557038.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         4785 a         5444 c         5861 g         4790 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgtggaaca tatccgtatt gtggatttcg gatgggtcgg tgtcgtcggg
      481 ataacggtat gtgccgtcag gaatatcgga agcaggtttt ccgtttaccg tcaaaacttt
      541 attccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggttgcaaa ggataattaa aaacgaccac atcgccccgt tcgattttgc ccgtggggat
      661 gaatacattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgccctta atcaggcccg ggcgcatcga gctggacggg atttggaacg gttcggcaat
      781 aaacgaccgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcataat gttcattgtt gttccttaac ggttaaaaac
     1141 ccgcccgtcc gtgcaaccgt ttttaagagg cggtaaatca caaagtttgt tggcggacgt
     1201 gctctcttac aatcagggcg gtttaagggg catgatgcac tgccccgtgt gccggatatt
     1261 atttgtcgct cacctgcaaa attgccaaga acgcgctttg cgggatttcc acattgccca
     1321 cttgtttcat acggcgtttg cctgcttttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttt gccaaaacgt ttttacgcag ggctttgacg ttctcacggg
     1441 cgataatctg actgccgatg gcggcttgga cggcaatatc aaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agttcgcggc cccggtgaac cgcgctttga cggtggacaa
     1561 tcaggctcag cgcatcgact ttttcaccgt tgaccatgat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata gccgcgcgaa gtagatttga
     1681 gtttgtcgaa aaagtccatc accacttcgt tcatcggcaa gtcgtaagtc agcatcactt
     1741 ggcggcccat atattgcata ttgacttgca cgccgcgctt ttggttgcac aaagtcatga
     1801 cgttgccgac atattcctgc ggcacgagga tggttgcagt gataatcggc tcgagaatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acggattttc gacttcgatt ttctcgccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaagtcc atgtcgaact
     1981 cgcgttccaa acgttcctgc acgatttcca aatgcaacag gcccaagaag ccgcaacgga
     2041 agccgaaacc caatgcttgg gaaacttcag gctcgaattt caacgaagca tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacc ggataaagtc
     2161 cggcgaatac ctggctttgc acttcttgga agccgggcag cggctcggag gcggggttgg
     2221 caatcaaagt aactgtatcg ccgacttttg cctgtcccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgatttcggc gtgaatacgc
     2341 ccagctgctc aacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagct
     2401 tgatggtgcc gtttttcaca cgaatcagca taaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacaat caccgcttgc agcggcgcat tttcatcgcc ggccggtgcg gggattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cattgcaccg
     2581 cgccgacggc atcgatgccg atgatatctt cgatttcctg ttccacgcgt tcggggtcgg
     2641 cggcgggcag gtcgattttg ttcaaaacgg gcacgacttc aacgcccaaa tcaatcgcgg
     2701 tgtagcagtt cgccacggtt tgcgcttcca cgccttgaga tgcgtcaacc accaaaagcg
     2761 cgccttcgca ggccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcag gttgagctga tacacctgcc cgtcgcgcgc tttgtagttg agcgcggcgg
     2881 tttgcgcttt gatggtgatg ccgcgctctt tttcgatgtc catagaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aagccgccgc agtattggat aaaacggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagtta
     3061 ttttgaatgt cggacagtgg gttttggaaa tgccgtctga acaaacggcg ctgcgtccga
     3121 atgtcgggcg caacgggata aacagcccga cattctagcg gaaaaccgct gttttggcat
     3181 gatttgataa aggttttata aagatttgac gatttctgcc accatttttg cagaacttgc
     3241 cgccgccgtt ttcaaaaatt cgtcaaagct gatgtctgcc ttttcgtccg ccgaatcgga
     3301 aaccgcgcgg atgatgacga aaggcgtttc caactgatga caggtttggg caatcgccgc
     3361 cgcttccatt tccactgctt tgactttggg gaagtgcttg cggatttccg ccacgccttc
     3421 gctgctgtgg acgaagcggt cgccgctgac aatcaggcct tgttctaccg ccgcgccttc
     3481 aaacacttcc gccgcctgtt ttgctttgcc gaccaagagg ccgtctgaaa caaatacggc
     3541 gggcagttgc ggcacttgtc cccaaacata gccgaatgcg gttacgtcga catcgtggtg
     3601 tgcggtttcc gtgccgatga ctacgtcgcc gactttcaaa cccttgccca aaccgcccgc
     3661 gctgccggtg ttgatgacgc agtccgctgc gaattcacgg ataatccaag ccgttgcaac
     3721 cgccgcgttg accttgccga tgccgctcaa tgcaagcacc atgcgttttc ccgccaattc
     3781 gccttcatag gcggaaaatc tgccgaaaga gacggctttg atattttcca tcatctcgcg
     3841 caaaagcttg atttcctgtt ccattgcgcc gataacggct actgttttca aagacatatt
     3901 gctgacctgc tgtgaatttc ggatagaatg ccgaattata cacgctaaca cggcaggatt
     3961 gagtggaggt ggtttgtccg tgccgtctga aacggtttca gacggcacgg cgggtttttg
     4021 gtagaatggg aaggtacaga ttgtttgaag attaggggac gaggatgttt accgatgaaa
     4081 atatgaccgc aaaggaagaa ctgttcgcat ggctgcgcca tatgaacaaa aacaaaggtt
     4141 ccgacctgtt tgtgacgacc catttcccgc ccgcgatgaa gctggacggc aaaatcaccc
     4201 gcatcacgga cgaaccgctg acggcggaaa aatgtatgga aatcgccttt tcgattatga
     4261 gtgcgaagca ggcggaagaa ttttcatcga ccaacgagtg caacttcgcc atcagcctgc
     4321 tggacaccag ccgcttccgc gtcaatgcga tgatacagcg cggcgcgacg gcgttggtat
     4381 tccgtgcgat taccagcaag attcccaagt ttgaaagcct gaacctgccg ccggtcttga
     4441 aggatgtcgc gctgaaaaaa cgcgggctgg ttatttttgt cggcggcacc ggctcgggca
     4501 aatcgacttc gcttgcctcg cttatcgatt accgcaatga aaattcgttc ggacacatca
     4561 tcaccatcga agacccgatc gagtttgtcc acgaacacaa aaactgcatc atcacccagc
     4621 gcgaggtcgg cgtggatacg gaaaactgga tggcggcgtt gaaaaacacg ctgcgtcagg
     4681 cgccggatgt catccttatc ggcgaaatcc gcgaccgcga aacaatggac tacgccattg
     4741 cctttgccga aacggggcat ttgtgtatgg cgacgctgca cgccaacagc accaatcagg
     4801 cactcgaccg catcatcaac tttttccccg aggagcggcg cgaacaattg ctgacggatt
     4861 tgtcgctcaa ccttcaggcg tttatttcgc aacgcctcgt tccgcgagac ggcggcaagg
     4921 gcagggtggc ggcagtcgag gtgctgctca attcgcccct gatttcggag ttgattcaca
     4981 acggcaacat ccatgaaatc aaagaagtga tgaaaaaatc cactaccctg ggtatgcaga
     5041 ccttcgatca acacctttac caattgtatg aaaaaggcga tatttccctg caagaagcat
     5101 tgaaaaatgc cgattccgca cacgatttgc gtttggcggt acagttgcgc agccgccgcg
     5161 cgcaaagttc cagccccgat ttggaactgc tctgatggcg gtatggattt ccggacggat
     5221 ggtttgaaat gatttatccg tggcataatg agcaatggcg gcagattgcg gaacattggg
     5281 agcgtcgtcc caatgcatgg ctgtttgccg gcaaaaaaga tacggggaaa actacatttg
     5341 cccgctttgc ggcgaaggca ctgttgtgcg aaacccctgc accgggctgc aaaccctgtg
     5401 gcgaatgtat gtcctgccat ctgtttggac agggaagcca tcccgatttt tacgaaatca
     5461 cccccttgtc ggacgaaccc gaaaacggac gcaaactgtt gcagatcaaa atcgatgccg
     5521 tcagggaaat catcgataat gtgtacctga cttcggtacg gggcggtttg cgcgtgattc
     5581 tgattcatcc tgcggaaagt atgaatgtcc aagccgccaa cagtttgttg aaagtgttgg
     5641 aagagccgcc gccacaagtg gtctttttgc tggtcagcca cgcggcggac aaggttttac
     5701 cgaccattaa aagccgctgc cggaagatgg ttttgcccgc gccttcccat gaagaggcat
     5761 tggcatatct gcgtgaaagg ggtgtggcgg aacctgagga acgtctggct ttccattccg
     5821 gagcgccgct gtttgatgag gcggacggtg tccgtgcgtt gcggattaaa ctgttggata
     5881 ttttggcaga accaaggttg ttgaagattt tggattacgc cgcgcttttc gataaggaaa
     5941 aacttccgct cgccgtattt gtcgggtgga tgcagaaatg gctggtcgat ttgggattgt
     6001 gcctgcaaca catgaaaccc gtctattatc ccgcttatga agacaggctg cttcagacgg
     6061 tatccggttt ccgcctgcgc aatgtatttg cggcggagga tatgctcaaa cagcttgccc
     6121 cctacgggtt tcatacttta aatgtcaaaa tgcagatcga gcatctgctc atcaactatt
     6181 tggaattgaa gaaagagaac gggtgaatta tgtcagacgg acaaaatatt ccggcaaaaa
     6241 tgatgtcgtt gcagctgaaa gacatgaatc tgctgtacag ctcctacatg ccgtttttgg
     6301 aacacggcgg tctgtttgtg cagaccaacg acgtattttc catcggggac gatattctgc
     6361 ttgccgtaga aatcctcaac ttccccaaac tgttcctgcc gaccaaagtc gcctggatca
     6421 atcctgcgcg tacttcctcc aaacccaaag gggtggggct ggcattcaca aaacacgaaa
     6481 actgcctgaa agtcaaagac cagatcgaag tcgaactggg caacacaatc ggcggcagca
     6541 gacctacgtt taccatgtaa cgccatgcat atcatcgatt cgcactgcca cctcaatttt
     6601 gaaggtttga aagaacgcct gcccgaagtt ttgtccaaca tggaagcaaa cggcgtgggg
     6661 caggcgctcg ccatcagcgt cagtagggaa agcttctccg aagtctttgc catcgccgaa
     6721 gcgcacgaac acatctattg caccataggc gtacatcccg acagcaagga agccgaagaa
     6781 ttttctattg cggaaatggt tgccgcagcc gcctcgccga aagtggtcgg cattggcgag
     6841 acgggtttgg attattactg gtgcaaaggc gatttgtcct ggcaacacaa acgctttgca
     6901 gaccacatcg aagcagccaa tcaaaccgga ctgcccgtta tcgtccatac gcgtgatgcg
     6961 gcggcggaca ccttgtctat cctgaaagaa tgccgggtta attcgggcgt tatccactgt
     7021 ttttccgaag acatcggttt tgcccgtgca gcaatggatt tggggcttta tatttctttc
     7081 tcgggaatcg ttacctttaa aaacgcaccc ttggttcagg aggcggcaaa atacgttccg
     7141 gacgaccgca ttttggtgga aaccgatgcg ccgttcctcg cccccgttcc caaacgcggc
     7201 aggcagaacg aaccggcttt tgtgcgccat accgccgaac atatcgccaa attgcggaac
     7261 caaacattgg aacaggttgc ggcatatacg acggaaaact tttaccggct gtttaaaaaa
     7321 gtacccgata tgcggaccgt ctgaccctgt accgacgata aggaaaacca tgaaggcaat
     7381 tcatccgtat gcatgtccgc gctgctgccg gctgcctgcc aacacgtttc ggacaggcat
     7441 ggcaaattcc gcttccaaat tctgcattgc caaaggcggc agacgggaag taaaaaaaga
     7501 cgaaagcggc ggcggatatg ccctgtgcca tttgccggac agcaggattg tcgaggagtg
     7561 ggaatatttc cgttcacaac attgatactg cgcgatatac ggcaaatatt gtgggaagtt
     7621 tccgcttttg cgtataatgc gtcctacctg acaaattttg tcaactttat caaaaggaat
     7681 aagcgatggc ttccatccac gaccaaatta aagaagtagt aacgacacac cgcgtcgtat
     7741 tgtttatgaa aggtacgaag cagtttccgc aatgcggttt ctcttcccgc gccgtgcaaa
     7801 tcctgaacgc ggcaggctgc accgattacg ttaccgtcaa cgtattggaa aatcccgaag
     7861 tacgccaagg cattaaggaa tacagcgact ggccgaccat cccccaactt tatgtgaacg
     7921 gcgagtttgt cggcggttcg gacatcctga tggaaatgta tgaggcaggc gagctgcaag
     7981 agctgctgaa agcctgatgg attcggcaat gccgtctgaa cgtgtttcag acggcatttt
     8041 cttttccggc aaatcaaaaa aaagtataat ggcgcgtctc aaaatcacac tggaacaccg
     8101 cgatgaacgt taatgttatc aaccatccgc tcgtccgcca caaattaacc ctgatgaggg
     8161 aggcggattg cagcacctac aaattccgga cgcttgccat cgagctggcg cgcctgatgg
     8221 catacgaggc aagccgtgat tttgaaatcg aaaaatacct tatcgacgga tggtgcggtc
     8281 agattgaagg cgaccgcatc aagggcaaaa cattgaccgt cgttcccatc ctgcgtgcag
     8341 gtttgggtat gcttgacggc gtactcgacc tgattccgac tgccaaaatc agcgtagtcg
     8401 gactgcagcg cgacgaggaa acgctgaaac ccgtttccta cttcgagaaa ttcgtggaca
     8461 gcatggacga acgtcctgcc ctgattatcg acccgatgct ggcgacgggc ggttcgatgg
     8521 ttgccaccat cgaccttttg aaagccaagg gctgcaaaaa catcaaggca ctggtgctgg
     8581 ttgccgcgcc cgagggtgtg aaggcggtca acgacgcgca tcccgacgtt acgatttaca
     8641 ccgccgcgct cgacagccac ttgaacgaga acggctacat catccccggc ttgggcgatg
     8701 cgggcgacaa gattttcggc acgcgctaac tgactgattt tcggagttga tatgaatttt
     8761 caagactatc tcgccacatt tccttcaatc gaccatctgg gcggtttgga cgttcaggat
     8821 gcagatggca aaacggttca ccacattcct gccgttcagg gtaagctcgg ttcgctcaag
     8881 ttgtacaacg ctttggcaga acgttttgac ggaaaattgg acaaagaagc ggcagaacag
     8941 ggtttgatat ggtttgccga acacgttgcc gacgcgcgcg cccatcccgg caagcatccc
     9001 aatatcgacc tgcttgaaaa agttgtgcaa agcggcgaaa ccttgctgct caagccgctt
     9061 gccgcgcaat aattttcgac catgccgtct gaaatccgtt tcagacggca ttttgtcgga
     9121 aagaagaccg taaaacgggc attttctttt ctatttcagg atacgggcaa tgatgtttca
     9181 acacacagga cgacacataa agcgttgccc tatgtgttgc cctaatttgg aaggggttac
     9241 acccctctca aataacatct gatgctgctg ccacgaagga cggatgtcca aatggcggag
     9301 tttcaaccat taaggaaata cgatgaaaaa aatgttcctt tctgctgtat tgcttctgtc
     9361 ggctgccgcc caaacggtgt gggcggatac ggtgttttcc tgtaaaacgg acaacaacaa
     9421 atatatagaa gttcaaaaaa tcaaccgcaa tctttacgaa tattcgttcg gcagtgcggc
     9481 aaaaaaagaa attgccatac gcaacagcaa agctgacctg ttggggcgtt ccgacaggtg
     9541 gcaaggcatg ggtagcggac gccgggcaac gatgaaattc caaaacggcg aatttatgta
     9601 caccgtatgg acgggcttcg attccgtgac tcatacggaa agcagcggtg ttgttgtgga
     9661 gcgtaggggc aaggaagtcg cacgggttgg ctgtacgccg caaaccgcgc aggcgaattt
     9721 caacgatgct gatttttccc ggtaatcggg gcggataagg cgatggaaac agtgaaaccc
     9781 atcatgctga ttgtccgacc gtcgggcagg gcgaaggacg atgtggaaac ctgccgtcgt
     9841 gccggatggc aggcggaagt gttgagcccg attgaaatcg aagcagatgc tgccggactg
     9901 gaacttttgt ccgaacaata tgcccgtgcg gatgccgtgt tttgggtcag tccgaccgcc
     9961 gttgaaaccg ccgtcccgta ccttaacctt tcagacggca taaagatgca catttccgta
    10021 gggcagggca gccgccgcgt attggaacgc tgtttgggca gaacggtcat cgcgcctgaa
    10081 gacggcaacg acagcgaggc ggttttgcgc ctgacggttt ggaacagtct gcccgaaggt
    10141 gcgcgcgtat tgtttgtgcg cggacacggc gggcgggatt ttctgatgaa tgccttgcag
    10201 gagaaaggtt ttcggacgga ggtggcagaa gtctatttca gacggcataa acctttgaac
    10261 tttcaaaatt tccaaaccga aaatattgcc gccgcctata ttacgtccac agaactcgtg
    10321 cgcttgctgt tcggacagct tccgccgcaa ttttcccgat tcttcaaatc cttgctatac
    10381 tttacccatc atccgcgcat tgcggaggca ttgaagcgcg aaggcgtgtg ttcggtcgaa
    10441 accgtcccta cgctggaagc cgcgctttcc cattcttcca tttccgtttc agacggcatg
    10501 gtctttcccg gaacctcaaa ttaataagga gcaaaacggt gggcgaacct gaaaacaaat
    10561 catccgaacc cgtacgcgag atacaggcat caaaagaaat gccgtctgaa acctcttccc
    10621 cacgcaaaga aaacgaaaca gaagtacaca ttcctgccgc tccttttatc gtcaaacagt
    10681 ccggcagcaa cgctttggca gtctgcgccc tggtattggc ggcattgggt ttgggtacaa
    10741 gtggtttttt gtttgtccaa ggacagaatg tcttgaaaaa ccaagagctg gcattcaacc
    10801 aaaaaatcga caaagccgcc ttgggcgagt cggaaaacgc cgccctgttg aaagacaacc
    10861 tcaaccggca agccgccata caatcagagc tcgaccgttt ggacggaaac gtcaaagcaa
    10921 acggcgaaca aatcttggaa atgcaaaaat cctatcgcga gttgaccaaa ggacgcgccg
    10981 attggctggt ggacgaaacc gagaccatac tcaatctggc ggcgcaacag ctggtgttga
    11041 ccggcaatat ccaaacggca gtcggcgtat tggagcatat cgacagccgc ctgtcccgtt
    11101 tcgatcaggc agagcttctg ccgatcaaac aggcagtcag cagcgacttg gcggaactga
    11161 aaaaccgtcc ctatgtcgat atttccggca cggcattgcg cctcgacagg ctggaaaccg
    11221 ccgtatccgg actgccgctg attttggacg gcgtattgaa gccgggcgta caggtgaaga
    11281 acgaagccgc ttctgcttca tggtggcaga acgtatggga aaaatccctc ggcacattga
    11341 aggggctggt cgaaatccgc cgtttggaaa acaacgatgc catgctgatt tctcccgaac
    11401 aggcatattt tgtgcgtgaa aacctgcgcc tccgcctttt ggatgcgcgg actgcattaa
    11461 tgcagcgcaa cagcgaagtc tatcagggcg atttgaacaa tgccgaagcc gccgtcagac
    11521 agtatttcga tgccaagtct cccgccacgc agtcgtggct gaaagaactg gcggaattga
    11581 aggcgttgga tgtgcgcatg acggcggatg acggtttgaa aaacagccta aatgccgtcc
    11641 gcgcctatcg cgacggtacg cgcatgacgg cggcggaaaa tcaagaagca gaacaggcgg
    11701 cttccgaacc ggcaaacgaa aacacagctt ccgaaccggc tgccgcatcg gatgtgaaga
    11761 ccatagaagc accgtccctg ccttcggaac gcaaaccgga acagcctgca aaaaaacagc
    11821 ccgtaccgga aaaggcaggg cgttcgccgt ccgctaaagg agaacgcgca tgaaaacggt
    11881 agtctggatt gtcgtcctgt ttgccgccgc cgtcgggctg gcattggctt cgggcattta
    11941 caccggcgac gtgtatatcg tactcggaca gaccatgctc agaatcaacc tgcacgcctt
    12001 tgtgttaggt tcgctgattg ccgtcgtggt gtggtatttc ctgtttaaat tcattatcgg
    12061 cgtattcaat atccccgaaa agatgcagcg tttcggttcg gcgcgtaaag gccgcaaggc
    12121 cgcgcttgcc ttgaacaagg cgggtttggc gtattttgaa gggcgttttg aaaaggcgga
    12181 actagaagcc tcacgcgtgt tggtcaacaa agaggccgga gacaaccgga ctttggcatt
    12241 gatgttgggc gcacatgccg ccgggcagat ggaaaacatc gagctgcgcg accgttatct
    12301 tgcggaaatc gccaaactgc cggaaaagca gcagctttcc cgttatcttt tgttggcgga
    12361 atcggcgttg aaccggcgcg attacgaagc ggcggaagcc aatcttcatg cggcggcgaa
    12421 gatgaatgcc aaccttacgc gcctcgtgcg tctgcaactt cgttacgctt tcgacagggg
    12481 cgacgcgttg caggttctgg caaaaaccga aaaactttcc aaggcgggcg cgttgggcaa
    12541 atcggaaatg gaacggtatc aaaattgggc ataccgccgc cagctggcgg atgctgccga
    12601 tgccgccgct ttgaaaacct gcctgaagcg gattcccgac agcctcaaaa acggggaatt
    12661 gagcgtatcg gttgcggaaa agtacgaacg tttgggactg tatgccgatg cggtcaaatg
    12721 ggtcaaacag cattatccgc acaaccgccg ccccgagctt ttggaagcct ttgtcgaaag
    12781 cgtgcgcttt ttgggcgaac gcgaacagca gaaagccatc gattttgccg atgcttggct
    12841 gaaagaacag cccgataacg cgcttctgct gatgtatctc ggtcggctcg cctacggccg
    12901 caaactttgg ggcaaggcaa aaggctacct tgaagcgagc attgcattaa agccgagtat
    12961 ttccgcgcgt ttggttctgg caaaggtttt tgacgaaacc ggagaaccgc agaaggcgga
    13021 ggcgcagcgc aacttggttt tggcaagcgt tgccgaggaa aaccgacctt ccgccgaaac
    13081 ccattgaaac cttcagacgg catcgttgaa atgccgtctg aaaaccattt cacttgacaa
    13141 acagactgac agaaaacccg tatgactctt ttaaaaaacg ataccttcct ccgcgccctg
    13201 cttaaacagc ccgtcgaata cacgccgatt tggatgatgc gtcaggcggg gcgttatctg
    13261 cccgaataca aagccacacg cgcgaaagcg ggcagcttcc tcgatttgtg caaaaacacc
    13321 gaattggcga ccgaagttac catccaacct ttggaacgtt tcgatttgga cgcggcgatt
    13381 ttgttttccg acatcctgac cgtccccgac gcaatgggct tgggactgta ttttgccgaa
    13441 ggcgaaggcc cgaaattcaa acgcgccctg caacacgagg acgacattgc caaattgcac
    13501 gtccccgata tggaaaaact gcaatacgtt ttcgacgcgg taacttccat ccgtaaagca
    13561 ttggacggcc gcgtaccgct tatcggcttc tccggcagtc cgttcacgct cgcctgttac
    13621 atggtcgaag gcggcagcag caaagaattc cgcaccatca aaaccatgat gtactcgcgc
    13681 cccgatttgc tgcacaaaat cctcgatacc aacgcccaag ccgttaccgc ctacctcaac
    13741 gcccaaatcg acgcgggagc gcaggctgtg cagattttcg acacttgggg cggcgtcttg
    13801 agcgatgcgg cgtttaaaga attcagcctc aaatacatcc gccaaattgt tgccggactg
    13861 aaacgcgaaa gcgaaggcag gcgcgtgcct gtcatcgtgt ttgccaaagg cggcgggctg
    13921 tggctggaaa gcatggcaga aatcggcgca gacgcattgg gcttggactg gacgtgcaac
    13981 atcggcgaag cacgccgccg cgtcggcaag caagtcgccc tgcaaggcaa cttcgacccg
    14041 tttgctctct ttggcacacc cgaatccatc cgcaccgaag tcgcgcgcat cctagccgac
    14101 tacggacacg gcagcggcca cgtcttcaat ctcggacacg gcatcaacca acacgccgac
    14161 cccgaacacg ccaaaatctt agtcgatacc gtacacgagc tgtctcggca gtatcacggc
    14221 gggtaagccg gcaggaaacc gcccgatatg ccgtctgaag ccgagagatg gccggttagg
    14281 gtaaaaataa ggcaatgcgg caatatccgc cgtgtatgga tagtacatga cggcggcggc
    14341 gttgtcgtat tggcgcaatc ccaaccgtcc ctattttcag acggcatttt tgttttcaga
    14401 tgcagggaaa accgatggca aaaacgctta aaacccttta ccaatgcacc gaatgcggcg
    14461 gcacctcccc gaaatggcaa ggcaaatgcc cgcattgcgg cgagtggaac acgcttcagg
    14521 aaagccttgc cgcgcccgag ccgaaaaacg cccgtttcca atcttgggcg gcggacactt
    14581 cgaccgtcca atccctctcc gccgttaccg caaccgaagt gccgcgcaat ccgaccggta
    14641 tgggcgaact cgaccgcgta ttgggcggcg gtttggtcga tggtgcggtc atcctgctcg
    14701 gcggcgaccc cggcatcggc aaatccacgc tgctgttgca aaccatcgcc aaaatggcgc
    14761 aaagccgtaa agtgctatac gtttccggcg aagaatccgc ccaacaagtc gccctgcgcg
    14821 cgcagcgttt ggaactgccg accgacggcg taaaccttct tgccgaaatc cgcatggaag
    14881 cgattcaggc ggccttgaaa cagcatcagc ccgaagttgt cgtcatcgac tctatccaaa
    14941 ccatgtattc cgaccaaatc acgtccgccc ccggctccgt gtcgcaggtg cgcgagtgtg
    15001 ccgcccaact gacgcgcatg gcgaaacaga tgggcatcgc catgatactg gtcggacacg
    15061 tgaccaaaga cggcgcgatt gccggcccgc gcgtgctgga acacatggtt gataccgtgc
    15121 tgtatttcga gggcgaccaa cattccaact accgcatgat acgcgccatc aaaaaccgct
    15181 tcggcgcggc aaacgaactg ggcgtgttcg cgatgacgga aaacggtttg aaaggtgtgt
    15241 ccaacccgtc cgccatcttc ctcgccagct accgcgacga tacgcccggc tcgtgcgttt
    15301 tggttacaca ggaaggcagc cgcccgcttt tggtcgaaat tcaggcattg gtcgatgacg
    15361 cgcacggctt cacgcccaaa cgcctcaccg tcggactgga acaaaaccgt cttgcgatgc
    15421 tgcttgccgt gttaaaccgc cacggcggca tcgcctgttt cgatcaggat gtgttcctca
    15481 acgccgtcgg cggcgtgaaa atcggcgaac cggcggcgga tttggcggtc atcctcgcga
    15541 tgctttccag cttccgcaac cgccctatgc ctgaaaaaac cgtggttttc ggcgaaatcg
    15601 gcttaagcgg cgaagtccgc cccgtcgcac gcgggcaaga gcggctcaaa gaagcggaaa
    15661 aactcggctt caaacgcgcc atcgtcccca aagccaatat gccgcgcaac gccaaagagt
    15721 ttccgaacct gaaaatctac ggcgtttcga gtttgcagga agccatcgat atttgccgcg
    15781 acagcaggga ataaacggaa atgccgtctg aaatcgggtt tcagacggca tttggtttgt
    15841 ggcggattga aacaagaagg cataccggcg acagataaga tttgcggcaa agttgcctgt
    15901 gatgtggcaa aaacacacac gcccgtcatc cccgcaaggg tgggaatccg gaatcgtccg
    15961 tttcggcaat gattgaaaat cacggtaacc caaccgattg gattcccgac ttcgtgggaa
    16021 tgaggggcgt gtgcatttga tttccatccg ccatatgtcg gcgacgggct tattcgccta
    16081 cggttttttg tatcagtttt tcggcgtttg ccaaagtgtt tgccacttcg tcgaaaccga
    16141 tgcggctgcc ggcgatgagg gcgcgcgtat cggtataggc ggcgcgtact ttgccgtccg
    16201 tttcggtaac gaggacgcgt aggggcagtt gcagggcgaa ggcggggtct ttgaccatca
    16261 gcggcgtgcc ggctttgggc gtgccgaaga cgatgacttt tgccggctgc atcgttaagc
    16321 cgtttcggcg ggcggcttcc tgatggtcga tgacggcaaa aatgtccatc cctttgcttt
    16381 ttatggcggt ttcaaggcga ctgacggttt cgtcaaaact gtattttgag gtgagggtat
    16441 gcgtggtcat agcggtttcg ttttgggtgg acggttcgct ggcaggatgt gccgaagcgg
    16501 ttgaaatgca gagtgcggat gcggcaatca gggggagtat gtgtttcatc gtatttcctt
    16561 tttccttttt ggttgaaacg gtagaatcag actttattcg ggaggggtgt aaccctttcc
    16621 aaatcagggc aacacatagg gcggtgcttt atgtgtcgtg aaacatcatt gttccttatc
    16681 ggtttgttta tcaggcttcg gacggggcgg cgcgcgccgc gcccgatgtt gcgccgcgtg
    16741 ccggaacgcc gtatgccgtc tgaaagcctg tcctttcaga cggcattgcg tcatttcatc
    16801 ccttttttga gcaggtcttc ataaccgccg tgattggcaa catttgtata acctgctttt
    16861 ttcagctctt gaagggcggc ttcggcacgc cgtccgctgc ggcagtagag gttgaccggc
    16921 gtgtctttgt cgggcgcggc ttcgtgtatg cggcggacga tttggtcgac ggggatgttg
    16981 accgcgttgt gcaaatgccc ttcgctaaat tcctgttcgg aacggacatc gatccaaacg
    17041 gccggatgtt gcgcggtttg ggcggcggat acgggttttt gcggggctgc ctgcgcggca
    17101 aaggcggctg aggcaatgag tgcggcggta atcaggtgtt tgatattcat agggttttcc
    17161 tgcggttgtt gtccgaaagg acgggaagtt attttatctg ttccaaagcg gcggcatcta
    17221 tgtcccaacg ccaaacgccg ccgcctttgc catccaatcc gcgcaaaaac aggtagcgga
    17281 cggaaacggc ggcgggcggt tgtccgcgca gcttgaagta gcgtgcggcg gcaacggcgt
    17341 aaatcagtgc ctgaaggtaa tagtggtggt gtgcgacggc ttcgtccatt gcctgttgcg
    17401 tgtaggcgga tgcgtccgta ccgaggtggt ttgatttgta gtcgatgacg cagatattgc
    17461 cgtcggggtc ttggcagacc atatcgacaa agccgtttaa aaagccgttg acggtgtgga
    17521 agtcgagcgt ttcggcagcg gcacggcaga cttcgggcag cctgatgtcg tcgcgggcaa
    17581 accagtcgcg caggcgtttg aggctgaagt cttcggtgtg gagggtaaag cccatttcgg
    17641 gacagcggca ctcgggtgag atgtcggaca ggtcgtatgc ccccgtcagc ggcgttttgc
    17701 ggcaggcttc cgccatttcg gcaacggcgg gcagccatat ttcttcaaaa ccgtattttt
    17761 tcagcttgtc ggcaatgagg gtttcctgtc cggcggctgc ttgtccgaat ttgaaatctt
    17821 caagaatttc gtgcaggcac agccccgcct gcgtgccttt cggaaaatcg tgtatcgata
    17881 tgccgtctga agccgtcggc gtttcagacg gcatcgccgg caccgaggtt tcggcggcat
    17941 ccaaggacgg gcaggcatct tcttcgccgc cgtcgggcgt ttgggtatgg cggcttaagg
    18001 cggtaaagct agtgtggcgg acaaatcgga atccgcgttc gggaatgctg tttgcggcaa
    18061 attcggcggt ttgaccggcg ttgctgcgat aggcggcagg gggcggcgca ttttcggtga
    18121 aggcgaaatt tgtgccggaa ggggcgttgt ccgccacgcg ccgccagttg cgtttgagca
    18181 tcgcgatgcc gtctttttca cacgcatagg cacggcggac ggtttcacgg ctgtcttggg
    18241 gcgagccttc aatcaggtag gcgagggggt tgtcggcagt attggtggag tacgcggcgt
    18301 agatgttgag ctgttcctcg gcacgcgtca gcgcgacata aagcaggcgc aggcgttccg
    18361 ccatttcttc atcggcgtat tgtttctgtt cgtcttccga cagttgcgcc tttgccaaca
    18421 gttcggttcg gtttgcgcct tggtggagga tttgccagtc ggacggtccg gtatcttgcg
    18481 cgtcccacgc aaacgggcag tacaccagcg gatactgcaa acctttcgag gcgtgcatgg
    18541 taacgatttt gaccaaatct tcgtcgcttt ccagacggat ggcgcggttg tcgccgctgt
    18601 tgttttcggc aaggctgatt tggtcgccca gccatttgtg cagcgcggcg gggttgcggt
    18661 tttgcgcgtc ttcggcggca agcagttcga gcagttggaa ataattggtc agactgcgcc
    18721 cgttgttccg gcttaagagg cgcgtttcga tgccgtgtgt ttgggaaaat tgctgcatag
    18781 cggcgaaaat gccgtattta ttccagttgt cgagtgcggt tcgggcagat tccgcccaat
    18841 gcaaaatctc gctttcgttt tggttgaagt cgtgcaattg ctgcgcgtca taaccgaata
    18901 tgctgcttgt caggacaaaa cgcagcgttc cggcgcggcg cggttcgagc cagaagccga
    18961 tgagtgcgga cagggcggcg gcttcgggcg aggcgaacac ggattcgcgc gaaagcagga
    19021 cgctttgcac ctgccgtttt ttcagggcgg cggaaaccat caccgcctcg ttgtgcgtgc
    19081 gtaccagcac ggcaatatcg cccgactgca acgggcagcc tttgaaattc agacggcctc
    19141 tggcggcttc gttgagcgcg tgggcgattt cgtcggcgca atagtcggcg gcacggcggc
    19201 gcaaaacgtc tttgttggct ttttcattgt cgttttcgtg cagccaacga acctgtacgg
    19261 caggacgttc gggggacagc ctgctttcgg cacgcgccgc accgacttcc gaatagccga
    19321 tgttttccaa aacgaacggg cgttctttga ggcggaacag cgcgcctatg ctgccgataa
    19381 gcgcggcgtg gctgcggtag ttggtggcga gcgtgtagcg gtgccgcgcg tcttccgccg
    19441 cctgaaggta ggcgtaaatg tccgctccgc gaaagctgta aatcgcctgt ttgggatcgc
    19501 cgacgaggaa cagcggtcgg ttttgggcga tgaaaatctt ttggaagatt tcgtattgca
    19561 gcgggtcggt gtcttggaac tcgtcgatca gcgcggtttc ccagttttcg gcaacggcgc
    19621 gggcgagagt gtcggcgtgc ggattgtcgg tcagcgcggt gtggacatcg agcagcaggt
    19681 cgtcgaaacc gcgttcgcgg cgcgattttt tcatctcggc aaggctgcgg ttgaggtatt
    19741 cgattaaatc cagttgcagc cggatcattg ttgcttcttc cgcttcttcg agtgcgttca
    19801 aatcgcgccc gaagtctgcc agtttctgca attcggcaaa tactgccgca tcgggcgttt
    19861 tgcctttttt cagtccggct tcgagtttgt cggatgcaag tttcaagagt ctgtcgtgtg
    19921 tgtctttgtc cagaaagggc agttgtccgg cggcggattt ttgtgccagt tctttaaaaa
    19981 ggttgccgaa gctgtttttg cggtaactgt tgccgttgag gtcgggatga atgcgccaaa
    20041 agccggcttc cagttctggc agcaggcggc agatggtttg ccatgaggtt tcggcgttgc
    20101 gctgcgcctg tttcaaatcc gcctgcggac ggcggaaatt caggtacggg cgggaaagat
    20161 agcgcgaaat ttgggcaagg acggtttgcg gcacagcttt gcgtttaagc gccaatgcgg
    20221 caagcaccgg atcattgctg acgcgttccc gccaaaaatc ttgcgccggg ataagcaggc
    20281 ggtcgccgtc ttcttcggtc atttcgacat cgaacggtgc ttggcacagg aaggcgtagt
    20341 cgcgcaggat gcgctggcag aagccgtgga tggtatagat ggcggcgttg tcgaattgcc
    20401 cgatggcggc cttgaggcgg acaatcagac gcgtccggcc ctctttttgc aaagcctgtt
    20461 ttaagagttc gggcaggaag gtgtcgcctt cgtggtgttc ggcgcagtag gcggcaatgc
    20521 cgtctgaaag cgtgtcgtct ccaagtttgg caattccttt gctttctaaa acttgtaaca
    20581 catcgtccaa acgcccgcgc aggcgtgttt tcagctcggc ggtggcggct ttggtgaagg
    20641 ttacgaccaa tacgcgttcg acgttttttt gttctaatac gatcaggcgt gtaaacaggg
    20701 cggcaatgcc gtaggttttg ccggtgccgg cagaggcctc aatcaggttg gtgccggaaa
    20761 tggggacggt tagcgggtcg aatgcttgga tgcttgcaga catagtgcgc gctcggaaaa
    20821 cggttggacg gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
//
LOCUS       DAWKOD010000019        20870 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000019 DAWKOD010000000
VERSION     DAWKOD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.019"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(25..716)
                     /locus_tag="WJK73_000511"
                     /pseudo
     CDS             complement(25..716)
                     /locus_tag="WJK73_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(975..1694)
                     /locus_tag="WJK73_000512"
     CDS             complement(975..1694)
                     /locus_tag="WJK73_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557039.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFVPIIAAAAF
                     GGALGAWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGGLAGNLAWR
                     PGIHALGFGANAANNAVNSRK"
     gene            complement(1821..1979)
                     /locus_tag="WJK73_000513"
     CDS             complement(1821..1979)
                     /locus_tag="WJK73_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557040.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="WJK73_000514"
     CDS             complement(1976..2818)
                     /locus_tag="WJK73_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7557041.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDSIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLTQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CAPK"
     gene            2884..3027
                     /locus_tag="WJK73_000515"
     CDS             2884..3027
                     /locus_tag="WJK73_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557042.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="WJK73_000516"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="WJK73_000516"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ7557043.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3987..4445
                     /locus_tag="WJK73_000517"
     CDS             3987..4445
                     /locus_tag="WJK73_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7557044.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4510..5175
                     /locus_tag="WJK73_000518"
     CDS             4510..5175
                     /locus_tag="WJK73_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557045.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5238..6455)
                     /locus_tag="WJK73_000519"
     CDS             complement(5238..6455)
                     /locus_tag="WJK73_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7557046.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6597..7142
                     /gene="def"
                     /locus_tag="WJK73_000520"
     CDS             6597..7142
                     /gene="def"
                     /locus_tag="WJK73_000520"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ7557047.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7229..8155
                     /locus_tag="WJK73_000521"
     CDS             7229..8155
                     /locus_tag="WJK73_000521"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ7557048.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8234..9493
                     /gene="rsmB"
                     /locus_tag="WJK73_000522"
     CDS             8234..9493
                     /gene="rsmB"
                     /locus_tag="WJK73_000522"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ7557049.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9459..10058
                     /locus_tag="WJK73_000523"
     CDS             9459..10058
                     /locus_tag="WJK73_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ7557050.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10058..12175
                     /locus_tag="WJK73_000524"
     CDS             10058..12175
                     /locus_tag="WJK73_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ7557051.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAITVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12168..13445
                     /locus_tag="WJK73_000525"
     CDS             12168..13445
                     /locus_tag="WJK73_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ7557052.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13536..14723
                     /gene="dprA"
                     /locus_tag="WJK73_000526"
     CDS             13536..14723
                     /gene="dprA"
                     /locus_tag="WJK73_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ7557053.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14749..15210
                     /locus_tag="WJK73_000527"
     CDS             14749..15210
                     /locus_tag="WJK73_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ7557054.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15282..17588
                     /gene="topA"
                     /locus_tag="WJK73_000528"
     CDS             15282..17588
                     /gene="topA"
                     /locus_tag="WJK73_000528"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ7557055.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPSRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17661..18356)
                     /locus_tag="WJK73_000529"
     CDS             complement(17661..18356)
                     /locus_tag="WJK73_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557056.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADETAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18376..18927)
                     /locus_tag="WJK73_000530"
     CDS             complement(18376..18927)
                     /locus_tag="WJK73_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557057.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19046..19424
                     /locus_tag="WJK73_000531"
                     /pseudo
     CDS             19046..19424
                     /locus_tag="WJK73_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            19513..20082
                     /gene="rsmD"
                     /locus_tag="WJK73_000532"
     CDS             19513..20082
                     /gene="rsmD"
                     /locus_tag="WJK73_000532"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ7557058.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20205..20456
                     /locus_tag="WJK73_000533"
     CDS             20205..20456
                     /locus_tag="WJK73_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ7557059.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            20553..20636
                     /locus_tag="WJK73_000534"
     tRNA            20553..20636
                     /locus_tag="WJK73_000534"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20587..20589,aa:Tyr,seq:gta)
     gene            20667..20740
                     /locus_tag="WJK73_000535"
     tRNA            20667..20740
                     /locus_tag="WJK73_000535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20699..20701,aa:Gly,seq:tcc)
     gene            20750..20824
                     /locus_tag="WJK73_000536"
     tRNA            20750..20824
                     /locus_tag="WJK73_000536"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20782..20784,aa:Thr,seq:ggt)
BASE COUNT         5257 a         5281 c         5660 g         4672 t
ORIGIN      
        1 aataaatggg caaaaatcgg tttattatcg tttttgccgc atttggattt gttctaccgt
       61 aaaacgtgtt tgacgaacgg gattcttatt aaaaaacatc tgatttctaa caaaatcagt
      121 attttttggc acgatggcta aaatttttcc ttccatttcg ccatcacgtg ttttccatgc
      181 gctcaagaat tgtgatttgc tcattgagac gtgccccagc gatggatcag ccagcaaaac
      241 agtttctccg ttaataccgt tcaataccga aaaatggttg tttttacggt attttaaata
      301 cacaattaca ggaattttta gttgtaccaa ctgttcaaat ggcaaagcat aaccttgtgc
      361 ttcaaaaccc agttcgggca ttatgcgttg catatcatca aaagaagcac gcatttgggt
      421 tttatccatt ttgtccaaga tttccgcttc agaataatgt ctgccataaa aattattcag
      481 taacgtggca atcgaagccg cgccgcaaga aaaatccaaa tcttgtttta ctatgccgga
      541 atctcgccgt gctttccaac tccgtacatg gatgttttgg taagaagcgg ggggtcaaca
      601 aacatgggcc aagcaaaaac tatatttggg gcgaaaccaa tcaaagccgc ataatttatc
      661 aatttataaa gattttttat cataatatgt atacgcggaa taaacgcatg aaacataaaa
      721 aaataaaatc atatgcaatt ttattgcatg aataaatatg aataaaatag ataatgatgg
      781 gagtaaatac gccatgtatt ttggaagttt aaatttatta ataataaaat aatttatcgt
      841 agcgcaaata aatcccaaaa ttggaacgat tagaaaaaaa attaatacac ccatcatcat
      901 gcaaactcta tattaataaa tagctaacta attctattgt ggaaattagt tagctaacta
      961 aaagttatta atgattattt tcgagaattg actgcattgt tggcagcatt ggcaccaaaa
     1021 cctagtgcat gaatacccgg tctccatgcc aaattcccag ccaatccgcc tccggcagca
     1081 gcagccagcc ctgttgccgc tactcctgtt gccgcaccga ttcctgccgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata cccccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctagtttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaacatt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgtact ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg tgcaggcggc gagtgccgac agggtgcaga tggggagggg tgttttcatt
     1981 tgggggcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccagca ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgtc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttggggat tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gctgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctggctcga ttatacctta tgcttatgca ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgacgtata accggatacc cgcctgacgc gggtttttta cggaaggggg gcaaaaatgc
     3061 ctaatccgct ttacagacag catatcatct ccatttcgga tttgtcgcgc gaacagttgg
     3121 aatgcctgct tcagacggca ttgaagctga aggcgcatcc gcgcggcgat ttgttggaag
     3181 gcaaacttat cggttcgtgc ttctttgaac cgtccacgcg cacgaggctg tcgtttgaaa
     3241 cggcggtgca gcggctgggc ggcaaggtca tcggcttttc ggacggcgcg aataccagtg
     3301 ccaaaaaagg cgagacgctt gccgataccg cccgcatcat ttccggctac accgacgcaa
     3361 tcatccaacg ccaccccaaa gacggcgcgg cgcgcgtggc agcggagttt tcgcgcgtcc
     3421 ccgtcatcaa cgccggcgac ggcaccaacc agcaccccag ccagaccttg ttggatctgg
     3481 taacgattta tgaaacgcag gggcgtttgg acaagctcaa aatcgccatg gcgggcgact
     3541 tgaaatacgg gcgcaccgtg cattcgcttt gtcaggcgtt gaaacgctgg ggctgcgaat
     3601 ttgcctttgt ttcgccgccc agcctcgcga tgcccgacta cattaccgaa gagttggacg
     3661 aagccggctg ccgataccgt atcctcggca gtttggaaga ggcggcggaa tgggcggaca
     3721 tcctgtatat gacccgcgtc cagcgcgaac gtttcgatga acaggaattt gccaaaatcc
     3781 aaggcaaatt caacctcgaa gcgtctatgc tcgcccacgc caaaccgaac ctgcgcgtgc
     3841 tgcacccttt gccgcgcgtg gacgaaatcc atcccgatgt cgatgccacg ccgcatgcct
     3901 attatttcga gcaggcaacc aacggcgttt atgcgcgtat ggcgatattg tcgctggtgt
     3961 tgaacgaaga agtgtgagga accgatatgg aaaccccgaa actcagtgtc gaagccattg
     4021 aaaaaggtac ggttatcgac catattcccg ccggcagggg gctgaccatc ctgcgccagt
     4081 tcaaactttt gcactacggc aacgcagtaa ccgtgggctt caacctgccc agcaaaaccc
     4141 aaggcagcaa agacatcatc aaaatcaaag gcgtgtgctt ggacgacaaa gccgccgacc
     4201 gccttgccct gtttgcaccc gaagcagtgg tcaacaccat cgacaatttc aaggttgtgc
     4261 agaaacggca tttgaacctg cccgacgaaa tcgccgaagt gttccgctgt ccgaacacga
     4321 attgcgccgg ccacggcgag ccggtcaaaa gccggtttta tgttaaaaaa cacaacgggc
     4381 agacgcggct gaaatgccat tactgcgaaa aaacctacag ccgggattcg gtggcggaag
     4441 cctgacggat tcccttaaac cgagcgggcg gcatttcgtc tgccgcttgt tttgccaatc
     4501 tgaaatggaa tgatgatgca cgcttctgtc caaagccgtt tcgcaccgat actttatgtt
     4561 ttgattttct ttgccggttt tttgaccgcg caaatctggt tcaatcagaa agcctatact
     4621 gaagagctgc ctccgcttct gtccgcattg tccgccgtcg cgctggtgtg gctggcgtgg
     4681 gcgttcgtgt cggcgcgttc aaaggctaag gcggaaaagt tctaccgcga aaaaatgata
     4741 cagaacgaaa gcatacaccc cgtcctgcac gcttctttgc aacacttgga acacaagccg
     4801 caaatgctcg ccctgctggt caaaaaccac ggcaaaggta tggcggagca ggtcaggttc
     4861 aaggcagaag tgctgcccga cgacgaagac gcgcgcacga ttgccgccga gttggcaaaa
     4921 atggatatgt tcgcattggg gacggacgcg gtcgcctcgg gcgaaaccta tggacgcgtg
     4981 ttcgccgata ttttcgagtt gtcggcggct ttggaagggc gcgcgttcaa aggaatgttg
     5041 aaactgacgg cggaatataa aaacatcttc ggcgatgcct gccgttcgga aacggcgttg
     5101 gagttgggcg cgctcaatca ggcgttgcag gagatttcaa aaacatcgga aaagtccaaa
     5161 cggatatttt attgaagatg gaaaaatgcc gtctgaaacg gaaggtgttt cagacggcat
     5221 ttttgtcgga tgattaatta ttcggagcgg ttgaagccaa acttcacgcg gctgcggccc
     5281 tgatccggta tattgtccaa atcgcgtccc ggattggcgg cggtgtcgcc tacggaaata
     5341 tcggagatgt tttccaaaat gatggcggac gacaggtgtt cggaggtgcg gtaaaccatt
     5401 gccaagccca cttcttcggc aggagtggaa atcagctcga cggtatccct gcttttgaaa
     5461 ttgttggaga ggtcgacctg catcgttttc ttgcgtttgt agaggctcaa aaccgtgcct
     5521 ttgtccaaac cgtccgcctc gcctttgtcg atggtgatgg tttgaaactg gccggcaatc
     5581 cttgtgcctt caaacacgga aacgatttta gcctgaaccg ggcgggacgg ttcgtgcggc
     5641 atcatgttga agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt
     5701 tcggaaatgg cggtttcgac caccagcggc tgtatcgaag gggtgcgcag cggggtaatc
     5761 aaaggatggg tgcgggtatg gtattcgttg tctttcggcc gttctccagc ctgtttcgag
     5821 cgttgttcga ggacagagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact
     5881 tcctgcccga ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc
     5941 aggtagcgac ccggctcttt caggcctttg gtgtaaaccc tggtgccttt ggtgtacagc
     6001 agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg
     6061 atttgcgggt gccgcataaa gatgcggtag aagttgacat cgatggcggg aataccgtat
     6121 ccggacactt ccttatccgg actcattttg acgacgggga tgccgtctgt ctgttccaag
     6181 ccgaggcgcg gttcgccgtc aacgtggcgc aacaccaata cctggtccgg ataaatcagg
     6241 tcgggattgt ggatttgatc ccggttcgcg tcccacaggc ggccccattg ccacgggctg
     6301 tacaggtatt tgcccgaaat gccccacagg gtgtcgccct gtttgaccgt gtagcgttcc
     6361 ggcgcgttcg ggcgcacctc caaatttgcc gccaaagttt gtgttgagaa tgccatacct
     6421 gccgcgcaga gcagggttat aatacgacgt tgcataaccg ttccccttat ctgataaatt
     6481 tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc agcccctcgg gatgtgccgc
     6541 gtgataaaaa atgttccgca ttttaacatc gaattatccg caccatcacg gtaattatga
     6601 aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat ggctttattg aatatcttgc
     6661 aatatcccga cgagcgtctg cacacggtgg caaagcctgt cgaacaagtc gacgagcgca
     6721 tccggaagct gattgccgat atgtttgaaa cgatgtacga atcgcgcggc atcgggctgg
     6781 cggcgacgca ggtcgatgtg cacgagcgcg tggtcgtgat ggatttgacc gaagaccgca
     6841 gcgaaccgcg cgtgttcatc aaccccgtca tcgttgaaaa agacggcgaa accacttacg
     6901 aagagggctg cctgtccgtg ccgggcattt acgacaccgt aacccgcgcc gaacgcgtca
     6961 aggtcgaggc tttgaacgaa aaaggcgaaa agttcacgct ggaggcggac ggcttgctgg
     7021 cgatttgcgt gcagcacgag ttggaccacc tgatgggcat cgtgtttgtc gaacgcctgt
     7081 cgcagctcaa gcagggacgc atcaaaacca agctgaaaaa acgtcagaaa catacgattt
     7141 gacccttttg ccgtgccgtc tgaacgctgc aaagttttca gacggcacgg tcttgtccga
     7201 caaccttacg catacgcagg aatatgctat gaaagtcatc tttgccggca cgcccgattt
     7261 tgccgccgcc gccttaaaag ccgttgccgc cgccggtttt gaaatcccgc tggtgctgac
     7321 ccagcccgac cgtccgaaag ggcgcggtat gcaactgacc gccccgcccg tcaaacaagc
     7381 cgcgctggaa ctcggtttgc gcgtggcaca gcccgaaaaa ctgcgtaaca acgccgaagc
     7441 cctgcaaatg ctcaaagagg tcgaggcaga cgtaatggtg gttgccgcct acggtttgat
     7501 tctgccgcag gaagtgttgg atacgccgaa acacggctgc ctcaacatcc acgcttcgct
     7561 gttaccccgt tggcgtggcg cggcgccgat tcaacgcgcg attgaagccg gcgatgccga
     7621 gacaggcgtg tgtattatgc agatggacat cggtttggac accggcgatg tggtcagcga
     7681 acaccgctac gccatccaac cgaccgatac cgccaacgaa gtccacgacg cgctgatgga
     7741 aatcggtgcg gcggcggttg ttgccgattt gcaacagctt caaagcaaag gccgtctgaa
     7801 cgcggtcaaa cagcccgaag aaggtgttac ttacgcccaa aaattgcgca aagaagaggc
     7861 gcgtatcgat tggagcgaaa gcgcggcggt tatcgaacgc aaaatccgcg ccttcaaccc
     7921 cgtgcctgcc gcgtgggttg agtatcaggg caagccgatg aaaatccggc gggcggaagt
     7981 ggtggcgcaa caaggcgcgg caggcgaagt gttgtcctgt tcggcggacg gtttggtcgt
     8041 tgcctgcggc gaaaacgcgc tgaagattac cgaattgcag cctgccggcg gcaggcgtat
     8101 gaatatcgcg gcgtttgcag caggacggca tatcgaagca gggacgaagc tgtaaacctt
     8161 tttcagacgg catttgatcc gcaaacggga atgccgcctg aaaccatcag ttgaagaaag
     8221 cgaatcacat aatatgagta tggcacttgc ccaaaaactt gccgccgaca gcattgcggc
     8281 ggttgccgaa ggacgcaacc ttcaggacgt gttggcgcaa atccgcaccg cgcatcccga
     8341 ccttacggcg caggaaaacg gcgcgttgca ggacatcgcc tacggctgcc agcgttattt
     8401 gggcagtttg aaacatatgc tcgcgcagat gctgaaaaag ccgattgaca acccgcagct
     8461 cgaaagcctg cttttggcgg cgttgtacca actgcattac acgcgcaacg cgccgcacgc
     8521 cgtggtcaat gaggcggtgg aaagcatcgc gaaaatcggg cgcgggcagt accgttcgtt
     8581 tgccaacgcg gttttgcgcc gttttttgcg tgaacgtgaa aaattggcgt cttcctgcaa
     8641 aaaagacgat gtggcaaaac acaacctgcc gctgtggtgg gtggcttact tgaaaaacca
     8701 ttatccgaaa cactggcaca acatcaccgc cgcgctgcaa tcgcatccgc cgatgacgtt
     8761 gcgcgtcaac cgccgacacg gcaatgccga aagctatttg gaaaaactgg ctgcggaagg
     8821 tattccggcc agggcgttgg acgaatatgc ggttacgttg gaagaagccg tgccggtaaa
     8881 ccgtctgccc ggtttttcag acggcattgt ttcggtacag gacttcggcg cgcagcaggc
     8941 ggcgtatctc cttaacccga aagacggcga acggattttg gacgcgtgcg ccgcgccggg
     9001 cggcaagacg gggcatattt tggaactggc ggattgccgc gttaccgcct tggacatcga
     9061 tgcaggccgt ctgaagcgtg tggaggacaa tatcgcccgt ttgggctttc agacggcatc
     9121 ggcggcgtgt gccgatgcgc gggatttggc ggcgtggtat gatggaaagc ctttcgacgc
     9181 aatccttgcc gacgtgccat gcaccgcttc cggcgtggcg cggcgcaatc ccgacgtgaa
     9241 atggctgcgc cgtccgaccg acgcgctcaa aaccgcccgc cagcaggaag ccctgctgga
     9301 cgcactttgg cagacgctga aacagggcgg cagaatgctg cttgccacct gttccgtgtt
     9361 cgtcgaagaa aacgacgggc aattgcaaaa attcctcaac cgccatgccg atgccgaacc
     9421 gatcgaatcg cgggtgcttt taccgaacaa acaccaagat ggcttttatt acgcgcttat
     9481 tcaaaagcat taaacaatgg cttgtgctgc tgccgatgct ttccgttttg ccggacgcgg
     9541 cggcggaggg gatagatgtg agccgcgccg aagcgaggat aaccgacggc gggcagcttt
     9601 ccatcagcag ccgcttccaa accgagctgc ccgaccagct ccaacaggcg ttgcgccggg
     9661 gcgtgccgct caactttacc ttaagctggc agctttccgc cccgataatc gcttcttatc
     9721 ggtttaaatt ggggcaactg attggcgatg acgacaatat tgactacaaa ctgagtttcc
     9781 atccgctgac caaccgctac cgcgttaccg tcggcgcgtt ttcgacagac tacgacacct
     9841 tggatgcggc attgcgcgcg accggcgcgg ttgccaactg gaaagtcctg aacaaaggcg
     9901 cgctgtccgg tgcggaagca ggggaaacca aggcggaaat ccgcctgacg ctgtccactt
     9961 caaaactgcc caagccattt caaatcaatg cattgacttc tcaaaactgg catttggatt
    10021 cgggttggaa acctctaaac atcatcggga acaaataatg cgccgttttc taccgatcgc
    10081 agccatatgc gccgtcgtcc tgttgtacgg actgacggcg gcaaccggca gcaccagttc
    10141 gctggcggat tatttctggt ggatagttgc gttcagcgca atgctgctgc tggtgttgtc
    10201 cgccgttttg gcacgttatg tcatattgct gttgaaagac aggcgcgacg gcgtattcgg
    10261 ttcgcagatt gccaaacgcc tttctgggat gtttacgctg gttgccgtac tgcccggcgt
    10321 gtttctgttc ggcgtttccg cacagttcat caacggtacg attaattcgt ggttcggcaa
    10381 cgatacccac gaggcgcttg aacgcagcct caatttgagc aagtccgcat tgaatctggc
    10441 ggcagacaac gcccttggca acgccatcac cgtgcagata gacctcatca gcaccgcctc
    10501 cctatccggc aatatgggca gggtgctgga acattatgcc aacgggttta cacagttgtc
    10561 cctttacaat gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca
    10621 gccgtttccg gataaggcgc gttgggaaaa aatccaacag gcgggttcgg tcagggattt
    10681 ggaaagcata ggcggcgtat tgtacgcgca gggctggctg tcggcgggta cgcacaacgg
    10741 gcgcgattac gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt
    10801 cttaatcgaa aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca
    10861 gacctttttc ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt
    10921 catggcactg tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc
    10981 gaaggcggtg gcgcaaggcg atttcagcca aacccgtcct gttttacgca atgacgagtt
    11041 cgggcgtttg accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc
    11101 agacgagcgc aaccgccggc gcgaggaagc cgccagacat tatcttgaat gcgtgttgga
    11161 ggggctgacc acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa
    11221 agcggcggaa cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg
    11281 ttggcacggc gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc
    11341 agcggcaggt acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat
    11401 cctgctgggc aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat
    11461 tgacgacatc accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcaaa
    11521 acggctggca cacgaaatcc gcaatccgct cacgcccatc cagctttccg ccgaacgtct
    11581 ggcgtggaaa ttgggcggga agctggatga gcaggatgca caaatcctga cgcgttcgac
    11641 cgacaccatc gtcaaacagg tggcggcatt gaaggaaatg gtcgaagcat tccgcaatta
    11701 tgcgcgttcc ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt
    11761 gttggcattg tatgaagccg gtccgtgccg gtttgcggcg gagcttgccg gcgaaccgct
    11821 gatggtggcg gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc
    11881 cgccgaagcg gcggaagaag ccgatgtacc cgaagtcagg gtaaaatcgg aagcggggca
    11941 ggacggtcgg attgtcctga cggtttgcga caacggcaaa gggttcggca gggaaatgct
    12001 gcacaacgcc ttcgagccgt atgtaacgga caaaccggcg ggaacgggat tgggtctgcc
    12061 tgtggtgaaa aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc
    12121 gggtggggcg tgtgtcagaa tcatcttgcc aaaaacggta aaaacttatg cgtagcagcg
    12181 atattttaat tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg
    12241 acgaaggtta ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc
    12301 aggtacgccc ggcgatggtg ttgctggata tttggatgcc cgattgcgac ggcatcaccc
    12361 ttttgaagga gtgggcgaaa aacgggcagc tcaatatgcc ggtggtgatg atgagcgggc
    12421 atgccagcat cgataccgcc gtagaagcga ccaaaatcgg cgcgctcgac tttttggaaa
    12481 aaccgatttc cctgcaaaag ctgctgtctg ccgtcgaaaa cgcgttgaaa cacggtgcgg
    12541 cgaaaatcga aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaaa
    12601 tgaaccgtga ggtaggggct gcggtgaaat gtgcctctcc cgtacttttg acgggcgagg
    12661 caggttcgcc gtttgaaacg gtggcacgct atttccataa aaacggtacg ccgtgggtca
    12721 gcccggcaag ggtcgaatat ctgatcgata tgccgatgga actgttgcag aaggcggagg
    12781 gcggcgtttt gtatgtcggc gacatagccc aatacagccg caacatccaa gccggcattg
    12841 cctttattgt cggcaaggca gaacgctgcc gcgtcagggt ggtcgcatcg ggcagcaggg
    12901 cggcaggttc agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg
    12961 tcgtccgtat tccgccgctg cgtatgcagc atgaagatat tcccttcctg atacagggga
    13021 ttgcctgcaa tgtggcggaa agccaaaaga ttgcgcccgc cgcattcagt gaagatgcgc
    13081 ttgccgtgct gacccgttac gactggccgg gaaatttcga ccaactgcaa agcgtcgttg
    13141 caacgctgtt gttggaggcg gacggacagg aaatcggcgc ggcggcggtt tcttcccttt
    13201 tggggcagaa tgtgcctgcc gagggagcag aagatatggt gggcgggttt aatttcaacc
    13261 tgcccctgcg cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc
    13321 aagaaggtca gaatatgagc aaagtggcgc agaaagtcgg tttggaacgc acgcaccttt
    13381 accgcaaact caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag
    13441 aataggcccg gacggccggt tttccggctg cgggcttttg ttttcagacg gcatttggtg
    13501 caaatgccgt ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt
    13561 ggctgcaatt ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc
    13621 gtttcggcag cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac
    13681 ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg
    13741 cggcagaagc ggcattggaa tgggaaatgc gggacggatg ccgtctgatg ctgcttcagg
    13801 atgaagattt tcccgaaatg ctgacgcagg ggctgaccgc gccaccggtt ttgtttttgc
    13861 gcggcaacgt acaactgctg cacaaacctt ccgccgccat tgtcggcagc cgtcatgcca
    13921 cgccgcaggc aatgcggatt gccaaagatt tcggcaagtc gttgggcggg caaaatatcc
    13981 ccgttgtgtc gggtatggct tccggtatcg atactgccgc ccatcagggc gcactggagg
    14041 cagaaggcgg caccatcgcc gtgtggggga cgggtataga ccgcatttac ccgccgtcca
    14101 ataaaaacct tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag tttcccatcg
    14161 gtacgcgccc gtatgccggc aattttccgc gccgcaaccg cctgattgct gccctgtcgc
    14221 aagtaacgct ggtggttgaa gccgccttgg aatccggttc gctgattacc gccggcttgg
    14281 cggcagaaat ggggcgcgaa gtgatggcgg tacccggttc gatagacaat ccgcacagca
    14341 aaggctgcca caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc
    14401 tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg
    14461 atacgcccga cacgggccgc cgcgccgttc agacggcata cgccccgccg cctgccgcaa
    14521 aaatgccgtc tgaaggggcg gcaggcggca cagccgtcgg cggcatactg gataaaatgg
    14581 gtttcgaccc catccatccc gacgtgcttg ccggacagtt ggctatgcct gccgcagatt
    14641 tgtatgccgc actgttggaa ttggaattgg acggcagcgt tgccgcaatg cccggcggca
    14701 gataccagcg tatccgaact tgaacgcact ttatattaag gaacacgaat gaccgaagtc
    14761 atcgcctacc tcatcgaaca tttccaagat ttcgatacct gtccgccgcc cgaagacttg
    14821 ggtatgctgc ttgaagaagc gggtttcgat acgatggaaa tcggcaacac cctgatgatg
    14881 atggaagtat tgctcaacag ctccgaattt tccgccgaac ctgccgacag cggcgcattg
    14941 cgcgtgtaca gcaaagaaga aaccgacaac ctgccgcagg aagtgatggg gctgatgcag
    15001 tatctgattg aagaaaaagc cgtcagctgc gaacagcggg aaatcatcat ccacgcgctg
    15061 atgcacatcc ccggcgacga aattaccgta gataccgcca aagtgctgac cctgctgctt
    15121 ttatgggcaa acaagagcga gctgcccgtg ttggtcggcg acgagctgat gagcgcgctt
    15181 ttactcgaca acaaacccac gatgaactga agcggcttca gacggcccgc ccgaggccgt
    15241 ctgaaacgcc ggcatcaaaa ccaccatcca gagaacgaca aatggcgaaa aacctattaa
    15301 tcgtcgaatc cccgtccaaa gccaaaaccc tgaaaaaata tttgggcggc gattttgaaa
    15361 tccttgcatc ctacggacac gtccgcgacc tcgtccccaa aagcggcgcg gtcgatcccg
    15421 acaacggctt tgcgatgaaa taccaactca tcagccgcaa cggcaaacac gtcgatgcca
    15481 tcgtcgccgg tgccaaagaa gctgaaaaca tctacctcgc caccgacccg gatagggaag
    15541 gcgaagccat ttcctggcat cttttggaaa tcctcaaatc caaacgcggc ttgaaaaaca
    15601 tcaagccgca gcgtgtcgtg ttccacgaaa tcaccaaaaa cgccgtgctc gatgccgttg
    15661 cccatccgcg cgaaatcgaa atggacttgg tcgatgcaca acaagcccgc cgtgctttgg
    15721 actatttggt cggctttaac ctttcgccat tgttatggaa aaaaatccgc cgaggtttga
    15781 gcgcggggcg tgtgcaaagc cctgctttgc gcctgatttg tgagcgcgaa aacgaaatcc
    15841 gcgcgtttga agcgcaggaa tattggacgg tgcatctcga cagccacaaa ggccgcagca
    15901 agttcaccgc caaactcgcc caatacaacg gcgcgaaact cgaacaattc gacctgtcga
    15961 acgaagccgc gcaagccgac gtattgaaag aactcgaagg caaagaagcc gtcgttaccg
    16021 ccattgaaaa gaaaaagcgc agccgcaatc ctgccgcgcc gttcaccact tctaccatgc
    16081 agcaggatgc cgtgcgcaaa ctcggcttca ccaccgaccg caccatgcgt accgcccaac
    16141 agctttatga aggtatagac gtagggcagg gcgcgatcgg tctgattacc tatatgcgta
    16201 ccgacagcgt gaatttggcg gatgaagcgt taaccgaaat ccgccattac attgaaaaca
    16261 aaatcggcaa agaatatctg ccgagtgccg ccaaacaata caaaaccaaa tccaaaaacg
    16321 cccaagaagc ccacgaagcc atccgtccga cttccgtgta ccgcacgccc gaaagcgtca
    16381 aacccttcct gagcgcagac cagttcaaac tctatcaaat gatttggcag cgtaccgtcg
    16441 cctgtcagat gacgcctgcc aaattcgacc aaaccaccgt cgatattacc gtcggcaaag
    16501 gcgtattccg cgtaaccgga caagtgcaaa ccttcgcagg cttcttgagc gtttatgaag
    16561 aaagcagcga cgatgaagaa ggcgaagaca gcaaaaaact gcccgaaatg agcgaaggcg
    16621 ataaattgcc cgtggacaaa ctctacggcg aacaacactt taccactccg ccgtcgcgct
    16681 acaacgaagc cacgctggtt aaagccctcg aagaatacgg catcggccgc ccctcgacct
    16741 acgccagcat catctccacg ctcaaagacc gcgaatacgt tacccttgag caaaaacgct
    16801 tcatgcccac cgacacaggc gacatcgtca acaaattcct gaccgaacac ttcgcccaat
    16861 acgtcgatta ccacttcacc gccaaactcg aagaccagct tgacgaaatt gccgacggca
    16921 aacgccaatg gattcccttg atggacaaat tctggaaacc gttcatcaaa caagtggaag
    16981 aaaaagaagg catcgaacgc gccaaattta ccacgcagga acttgatgaa acctgcccga
    17041 aatgcggcga acacaaactg caaatcaaat tcggcaaaat gggtcgtttt gttgcgtgtg
    17101 ccggttatcc cgagtgcagc tacacgcgca atgtcaacga aaccgccgaa gaggccgccg
    17161 agcgcatcgc caaagccgaa gccgaacagg ccgaactcga cggacgcgaa tgtcctaaat
    17221 gtggtggtcg cctagtgtac aaatacagcc gaaccggcag caaattcatc ggctgcgcca
    17281 actatccgaa atgcaaacac gtcgagccgc tggaaaaacc gaaagatacc ggcgtccagt
    17341 gtccgcaatg caaaaaaggc aacctcgtcg agcgcaaatc ccgctacggc aaactgtttt
    17401 acagttgcag cacctatccc gactgtaact acgccacatg gaacccaccc gttgcagaaa
    17461 gctgtccaaa atgcgcatgg ccggttttaa ccatcaaaac caccaaacgc tggggcgtcg
    17521 aaaaagtctg cccgcaaaaa gaatgcggct ggaaagaaca gattgaaccg cctgcgccga
    17581 aagagtgaga ttaggttggt ttgaaagaga aaaggtcgtc tgaaaaattt tcagacgacc
    17641 tttgcttttc tgtgattggt ttatttgaat ccgcgtgttg ttttaaagtc cgataaaatc
    17701 cggttcattt caggcgcaaa caaggcgatg taatcgtaag atagaccgcg actggcactg
    17761 ggatggggaa agcagacgac ttcgcaatct tcaaacgatt ggaatttgac attgaaacgt
    17821 gtaccgtcaa attctttttg caccgtctcc agcggtttgg tctgcttacc gaccaactgc
    17881 tcgaagcgtg gcagtacatt ttggttgttc agaaaatccg ccaacctgct gcccatgaag
    17941 aggatgactt tcggacgcag tttttcgatg tggtagagaa aattatcgat gtgctcgggt
    18001 tgtgtgaact tgtcgggatt gtcgatagtg ttgccctgtg tagcagccca gttggtttga
    18061 accagggatt tttcaaatgc accgcccaat ccattttcgt ctaaggggtg tccccacatt
    18121 tcaaaccaat tttttatcgt attgtcgtaa cgccactttt ttgcctgctc tccgaaatag
    18181 agggatttgt ttgcaaatgt atggtcgatt ttgttttcag ggagtttgta ttcacctgct
    18241 acataagcag tctcatcggc tttactccaa ccccattcat agccacaaat cattaagcca
    18301 tgtttgtcgt tgtagccttt gaacaggctg ttgctcaaat tcaaatcctt catcatgaac
    18361 tcttcctttt aaaatttaag agcgattgac ttcaatgttt ttagatgggg tggaaaaatc
    18421 cttgtgtagg caacataaat tcaataaatt tcttgataat tcgaaaccta ctaatagcgc
    18481 acctataaaa gctttttcat tacgttcagc atgacggtca cgtcgttcat attttttacg
    18541 cttgctgttc cctgttatta cagctaagcc aagtgatatg gcgagaattg cccaaacaat
    18601 agtacttaat aacaattttc cccatactat caataaggaa agaaaaaacc ttttagtatt
    18661 agatcgatag gttataatcc atgcccatga aaatgcaata agagttatac ataagataca
    18721 agctgacagg atttctttat ttttaattaa ataacttaga gcgatgagga tgacaggtgt
    18781 caagaaaata atagttacat cccgatagct ataaaagaaa actgccctat tttgaatgtg
    18841 gagatgtgca cagaatccaa tatagctaag gataatagtt aatataataa aaaaagacca
    18901 ccaagggtga agagatagga attccatgtt ttcccttttt ttgtaaaaag gaaaaaatct
    18961 tatctaatag ttaattgcct aaccagccag aagaagttta aaatctatcc caataattca
    19021 accatctata cagaaagttc agcttatgga aacccacgaa aaaatccgcc tgatgcgcga
    19081 attgaataaa tggtcccagg aggatatggc ggaaaagctg gcgatgtcgg caggagggta
    19141 tgccaaaatc gaacggggcg aaacgcagtt gaatatcccg cgtttggagc agttggctca
    19201 gattttcaaa atcgatatgt gggacttgct caaatcgggc ggtggtggga tggtgtttca
    19261 gattaatgaa ggtgatagtg gtggcgatat ttgcgttgta tgcgtcgggt gatgtttcga
    19321 tgaaaataga atttttaaaa atggagttga aacactgcaa agaaatgttg gaacaaaaag
    19381 acaaagaaat cgagctgctc cgcaagctga ccgaaaccgt ttaaacagat atgccgtctg
    19441 aaaaaagttt tcagacggca tattctttga caggtcttgt ataataccgt ttgaacttac
    19501 aggtttttga ttatggcggc aggcaaacat accaaacaca gcaaccgggt acgcattatc
    19561 ggcgggcaat gccggggcag gaaattgagt ttcacatccg ccgacggact gcgtccgaca
    19621 cccgacagcg tgcgtgaaaa gctgtttaac tggctgggac aggatttgac gggtaaaacg
    19681 gttttggatc tcttcggagg cagcggcgca ctcggtatag aagccgcttc gcgcaacgcc
    19741 aaacgcgtgc tgatttcgga taacaaccgc caaaccgtgc agaccttgca gaaaaacagt
    19801 cgcgaactgg gtttggggca ggtgcaaatc gtctgttcag acggcatcgc atatttgaag
    19861 accgtatccg aacagtttga tgttgtcttt ctcgacccgc cgtttgcatg gcaggactgg
    19921 caaatcctgt ttgatgcctt gaagccgtgc ctgaaccccc gggcattcgt ctatctcgag
    19981 gcgggtacgc tgccggatat tcccgattgg ctgacggaat atagagaggg gaaatcgggg
    20041 cagagtacat ttgaattaag ggttttccaa gtggctgaat aatatgcgct ttgataatca
    20101 tttccgagtt gtaaacattc gtttgcaacc gtccggttca aaaaaacctt gtgctataat
    20161 ccgcgcccgc ccggttttga taatttagtg gaaaaggaaa agaaatgtcg ctttttatta
    20221 ccgacgagtg catcaactgc gacgtatgcg aacccgaatg ccccaatgat gccatttccc
    20281 aaggcgagga aatttacgaa atcaacccca acctctgcac gcagtgcgtc ggacactacg
    20341 atgagccgca gtgccagcag gtttgcccgg tggactgcat cctgattgac gaagaatatc
    20401 ccgaaaccca tgacgagttg atggcgaaat acgaaaagat tatccagttt aaataaattc
    20461 tttttaaaac atcaaattat gtctgttttg aaataaaatc aaaaaaaaac ttgacggaaa
    20521 agcaagccgc taataaacta acgttctctt ttggaggggt tcccgagcgg tcaaaggggg
    20581 cagactgtaa atctgttgcg aaagcttcga aggttcgaat ccttctccct ccaccaaaat
    20641 tcttacttgg ggcagtagcg agtaatgcgg gtgtagctca atggtagagc agaagccttc
    20701 caagcttacg gtgagggttc gattcccttc acccgctcca aacaattagg cccatgtagc
    20761 tcaggggtag agcactccct tggtaaggga gaggccggca gttcaaatct gcccatgggc
    20821 accatctctc gattattcat ttctttaagg cttagatata taggatattg
//
LOCUS       DAWKOD010000020        20300 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000020 DAWKOD010000000
VERSION     DAWKOD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.020"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(586..1728)
                     /locus_tag="WJK73_000537"
     CDS             complement(586..1728)
                     /locus_tag="WJK73_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7557060.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASVANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            2032..2295
                     /gene="rpsT"
                     /locus_tag="WJK73_000538"
     CDS             2032..2295
                     /gene="rpsT"
                     /locus_tag="WJK73_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ7557061.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2455..3603
                     /locus_tag="WJK73_000539"
     CDS             2455..3603
                     /locus_tag="WJK73_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ7557062.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3746..4447
                     /locus_tag="WJK73_000540"
     CDS             3746..4447
                     /locus_tag="WJK73_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ7557063.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKAELPEQQ"
     gene            4505..6313
                     /gene="aspS"
                     /locus_tag="WJK73_000541"
     CDS             4505..6313
                     /gene="aspS"
                     /locus_tag="WJK73_000541"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ7557064.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            6464..6748
                     /locus_tag="WJK73_000542"
     CDS             6464..6748
                     /locus_tag="WJK73_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7557065.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6915..8807
                     /gene="speA"
                     /locus_tag="WJK73_000543"
     CDS             6915..8807
                     /gene="speA"
                     /locus_tag="WJK73_000543"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ7557066.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8906..9829
                     /gene="speB"
                     /locus_tag="WJK73_000544"
     CDS             8906..9829
                     /gene="speB"
                     /locus_tag="WJK73_000544"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ7557067.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            10027..11493
                     /locus_tag="WJK73_000545"
     CDS             10027..11493
                     /locus_tag="WJK73_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ7557068.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11700..12797)
                     /locus_tag="WJK73_000546"
     CDS             complement(11700..12797)
                     /locus_tag="WJK73_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ7557069.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGKGNLRLGN"
     gene            13020..14162
                     /gene="bioF"
                     /locus_tag="WJK73_000547"
     CDS             13020..14162
                     /gene="bioF"
                     /locus_tag="WJK73_000547"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ7557070.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVYL"
     gene            14172..14819
                     /locus_tag="WJK73_000548"
     CDS             14172..14819
                     /locus_tag="WJK73_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ7557071.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELIALFAMIGQDRR
                     ADFIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            14807..15589
                     /gene="bioC"
                     /locus_tag="WJK73_000549"
     CDS             14807..15589
                     /gene="bioC"
                     /locus_tag="WJK73_000549"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ7557072.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            15809..17245
                     /locus_tag="WJK73_000550"
     CDS             15809..17245
                     /locus_tag="WJK73_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ7557073.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            17248..18345
                     /locus_tag="WJK73_000551"
     CDS             17248..18345
                     /locus_tag="WJK73_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7557074.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAVTYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            18357..18893
                     /locus_tag="WJK73_000552"
     CDS             18357..18893
                     /locus_tag="WJK73_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ7557075.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            18895..19539
                     /locus_tag="WJK73_000553"
     CDS             18895..19539
                     /locus_tag="WJK73_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ7557076.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            19562..19921
                     /locus_tag="WJK73_000554"
     CDS             19562..19921
                     /locus_tag="WJK73_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ7557077.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            20014..20089
                     /locus_tag="WJK73_000555"
     tRNA            20014..20089
                     /locus_tag="WJK73_000555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20047..20049,aa:Asn,seq:gtt)
BASE COUNT         5118 a         4716 c         5243 g         5223 t
ORIGIN      
        1 taaggcaatt tatcgggaat gactgaaact caaaaaactg gattcccact ttcgtgggaa
       61 tgacgggatg caggtttccg tgcggacgtg ttctagattc ccactttcgc gggaatgacg
      121 ggatttcagt ttgcggattg atttgaagtt gcaaaatccc aacggatcgg attaccgctt
      181 tcgcgtttca aagttacggc gttatcggaa aaacagaaaa tcaaagctgc aagaatttat
      241 ttaaaacaac cgaatttcaa cggatcggat tctcgcctgt ggggaatgac ggcggaaggt
      301 tttttgtctt ttctgacaga tgtccgcaat ctgaaatcct gaccgtggga acgacggtat
      361 agtggattaa caaaaaccag tacggcgttg cctcgcttta gctcaaagag aacgattctc
      421 taaggtgctg aagcaccaag tgaatcggtt tcgtactatt tgtactgtct gcggctttgt
      481 cgccttgtcc tgatttttgt taatccacta tataaatatt tctatttcaa tccaatataa
      541 aatgccgtcc gaacgatgtt cggacggcat ttttaatgct tcaaatcagt tggtgccgac
      601 tttgattttc tgccacaggc tgacagacag ttttttcgca tccgtactca tttgcggcat
      661 cacaaaaccg tctttcatat cctgctcgtt cgggaagatg gaacgggtgt tcaccagctc
      721 ggcaggcatt ttttcgcgcg ccggtttgct ggcgggggcg aaggttacgg cgatgccgtt
      781 tttcgccgcg atttcggggt cgagcgtgta gttgatgtat ttgtgggcat tggcgacgtt
      841 tttcgcatcg gcgggaatca gccaagactc aatccagaag cccatgcctt tcggattcag
      901 cacttcgatg ccgacgttgt ttttcacttc ttcggaacgt gctttcgcca agttcaaatc
      961 gccgccgttg cctgccgcca ggcagatgtc gccgcgcgcc agctcgtcga tgatggacgg
     1021 gctgaaacgt ttgacatccg gacggataga cttcaacact tccgccgctg ccttcaaatc
     1081 ttcaggattc gaacctttgg ggtctttgcc caagtagttc agcaaaatcg ggaacatttc
     1141 gctcggggtg tcccacaggg cgatgccgca ggatttcagc ttgtgggtgt attcgggttt
     1201 gaacagcaaa tcccagccgt tttcgggcag cttgccgcct aaaagctctt tgcctttcgc
     1261 cgtaatcgca atcgtgttca cgccggagaa ataggggacg gcatactggt tgcccgggtc
     1321 ggcggtttcc agcattttca agagttcggg atcgatgttt ttatagttgg gaatcaggtc
     1381 tttgttgact ttttgatacg cgcccgcctc gatttggcgc ggcaggaagg cgatgcccgg
     1441 cacaaccaaa tcgtaaccgg atttgccggt cagcattttg gcttccagcg tttcattgtt
     1501 ttcgtacaag tcgtaagtga gcttcagatt gttggctttt ttaaagtctt cgaccgtact
     1561 ctcgtcaaca tagttcgacc agttgtagat gttcagagta tcggtggcag cggcttcggc
     1621 attggcaaca gacgcagcgt ctgcttgagg ttgcacggcg tttttttcgc tgccgccgca
     1681 ggctgccaga gacagcgcgg ccaaaacggc taatacggat tttttcatac gggcagattc
     1741 ctgatgaaag aggttggaaa aaaaagaaaa ccccgcgccc cataaacacc ccggcgcaag
     1801 gtttgggcat tgtaaagtaa atttgtgcaa actcaaagtg atattggact gatttcccta
     1861 aaaaattacc ctgttttcaa aagaggggga aaacgtccgc ccgattttgc cgtttttttg
     1921 cgctgtcagg gtgtccgacg ggcggataga gagaaaaggc ttgcatataa tgtaaacccc
     1981 ctttaaaatt gcgcgtttac agaatttatt tttcttctag gagattccaa tatggcaaac
     2041 agcgcacaag cacgcaaacg tgcccgccag tccgtcaaac aacgcgccca caacgctagc
     2101 ctgcgtaccg cattccgcac tgcagtgaaa aaagtattga aagcagtcga agcaggcgat
     2161 aaagctgccg cacaagcggt ttaccaagag tccgtcaaag tcatcgaccg catcgccgac
     2221 aagggcgtgt tccacaaaaa caaagcggca cgccacaaaa gccgtctgtc tgcaaaagta
     2281 aaagcactgg cttgattttt gcaaaaccgc caaggcggtt gatacgcgat aagcggaaaa
     2341 ccctgaagcc cgacggtttc ggggttttct gtattgcggg ggcaaaatcc cgaaatggcg
     2401 gaaagggtgc ggtttttatc cgaatccgct ataaaatgcc gtttgaaaac caatatgccg
     2461 acaatggggg cggagatgaa tacacggaat atgcgctata ttcttttgac aggactgttg
     2521 ccgatggcat ccgcttttgg agagaccgcg ctgcaatgcg ccgctttgac ggacaatgtt
     2581 acgcgtttgg cgtgttacga caggattttt gcggcacagc ttccgtcttc ggcagggcag
     2641 gaagggcagg agtcgaaagc cgtactcaat ctgacggaaa ccgtccgcag cagcctggat
     2701 aagggcgagg cggtcattgt tgttgaaaaa ggcggggatg cgcttcctgc cgacagtgcg
     2761 ggcgaaaccg ccgacatcta tacgcctttg agcctgatgt acgacttgga caaaaacgat
     2821 ttgcgcgggc tgttgggcgt acgcgaacac aatccgatgt accttatgcc gctctggtac
     2881 aacaattcgc ccaactatgc cccgggttcg ccgacgcgcg gtacgactgt acaggaaaaa
     2941 ttcggacagc agaaacgtgc ggaaaccaaa ttgcaggttt cgttcaaaag caaaattgcc
     3001 gaagatttgt ttaaaacccg cgcggatctg tggttcggct acacccaaag atccgattgg
     3061 cagatttaca accaaggcag gaaatccgcg ccgttccgca atacggatta caaacctgaa
     3121 attttcctga cccagcctgt gaaggcagat ttgccgttcg gcggcaggct gcgtatgctc
     3181 ggtgcgggtt ttgtccacca gtccaacgga cagagccgtc ccgaatcgcg ttcgtggaac
     3241 aggatttacg ccatggcagg catggaatgg ggcaaattga cggtgattcc gcgcgtgtgg
     3301 gtgcgtgcgt tcgatcagag cggcgataaa aacgacaatc ccgatattgc cgactatatg
     3361 gggtatggcg acgtgaagct gcagtaccgc ctgaacgaca ggcagaatgt gtattccgta
     3421 ttgcgctaca accccaaaac gggctacggc gcgattgaag ccgcctacac gtttccgatt
     3481 aagggcaaac tcaaaggcgt ggtacgcgga ttccacggtt acggcgagag cctgatcgac
     3541 tacaaccaca agcagaacgg tatcggtatc gggttgatgt tcaacgactt ggacggcatc
     3601 tgaaccgcgt gttcagacgg tatatcaagt tgccgtgccg tctgaagccg ccggcggttt
     3661 ggcggggcgg gataaaaatt tcgtttgaat ccgggcaaac gcgtataatg tgcggtttgt
     3721 acggactttg tcggaatcaa gcaagatgac ggaacctgcg gccgaaggcg gtaaagctgc
     3781 caaggcgtta aaaaaatatc tgattacggg cattttggtc tggctgccga ttgcggtaac
     3841 ggtttgggtg gtttcctata tcgtttccgc gtccgatcag ctcgtcaacc tgctgccgaa
     3901 gcaatggcgg ccgcaatatg ttttggggtt taatatcccg gggctgggcg ttatcgttgc
     3961 cattgccgta ttgtttgtaa ccggattatt tgccgcaaac gtattgggcc ggcagattct
     4021 tgccgcgtgg gacagcttgt tggggcggat tccggttgtg aagtccatct attcgagtgt
     4081 gaaaaaagta tccgaatcgt tgctgtccga cagcagccgt tcgtttaaaa caccagtact
     4141 cgtgccgttt ccccaatcgg gtatttggac aatcgcattc gtgtccggtc aggtgtcgaa
     4201 tgcggttaag gccgcattgc ctcaggatgg cgattatctt tccgtgtatg tcccgaccac
     4261 gcccaacccg accggcggtt actatattat ggtaaagaaa agcgatgtgc gcgaactcga
     4321 tatgagcgtg gacgaagcat tgaaatatgt gatttcgctg ggtatggtca tccctgacga
     4381 cctgcccgtc aaaacattgg caggacctat gccgtctgaa aaggcggagt tgcccgaaca
     4441 acaataaagc cgccgttcag acggcatttt ctgttttcag tttaaatcaa taaaaggtga
     4501 ttttatgcgt accaactatt gcggtctgat tagcgagcaa tacttagacc aaaccgttac
     4561 cgtcaaaggc tgggtacacc gccggcgcga ccatggcggt gtgattttta tcgacctgcg
     4621 cgaccgcgaa ggcatcgtcc aagtcgtgat cgatcccgac acgcccgaag cgtttgccgc
     4681 tgccgactct tcccgcaacg aatacgtttt gagcattacc ggccgcgtac gcaaccgtcc
     4741 cgaaggcacg accaacgaca aaatgatttc cggcaaaatc gaaattcttg ccaaagaaat
     4801 cgaagttttg aacgccgccg ctacgccgcc gttccaaatt gacgacgaaa acatcagcga
     4861 aaacgtgcgc ctgaccaacc gcgttatcga cttgcgccgt ccggtgatgc agcgcaacct
     4921 gcgcctgcgt taccaagtgg ctatgggcgt tcgccgctac ttggacgcgc aaggtttcat
     4981 cgacattgaa accccgatgc tgactcgctc cacgcccgaa ggtgcgcgcg actacctcgt
     5041 gccgagccgc gtccatccgg gcgaattttt cgcgctaccg caatcaccgc aattatttaa
     5101 gcaactgttg atggtggccg gtttcgaccg ttactatcaa attaccaaat gtttccgtga
     5161 cgaagacctg cgtgccgacc gtcagcctga atttactcaa atcgacttgg aaacctcgtt
     5221 cttgaacgag gatgaaatca tggacatcac cgaaggcatg gcgaaacaag tcttcaaaga
     5281 cgcgctgggc gtggacttgg gcgacttccc acgcatgcct tactctgaag ccatgttcta
     5341 ctacggctct gacaaaccgg acatgcgtat caacttgaaa ttcaccgagt tgaccgacct
     5401 gatgaaaacg gaagaattca aagtcttccg tggcgcagcc gacatgaaag gcggccgcgt
     5461 ggttgccctg cgtgtgccga acggtgcgaa attcagccgc aaagaaatcg acgaatacac
     5521 taaatttgtc ggcatctacg gtgcgaaagg tctggcatac atcaaagtaa acgatgtggg
     5581 caacctttcc aacggcgaag acagcggcct gcaatccccg attgtgaaat tcctgtccga
     5641 aaacgccctg aaggaaatca ttgcgcgtac cggcgcggaa aacggcgaca tcatcttctt
     5701 cggtgcggac aaagccaaag tcgtgaacga agccattggc gcattgcgta tcaaagtcgg
     5761 cttggaacac ggcgcggaaa acggctattt cacagacgaa tggaaacctt tgtgggttgt
     5821 cgacttccca atgtttgaat acgacgaaga agccgaccgc tacgttgccg tacaccatcc
     5881 gtttaccgca ccaaaagaag gccatgaaga cctgatggtt tccgacccgg caaattgttt
     5941 ggcgcgcgcc tacgatatgg tattgaacgg ctgggaaatc ggcggcggct ctatccgtat
     6001 ccaccgcgca gacgtgcaag agaaagtgtt tgccgcgctg aaaatcagcc ctgaagagca
     6061 acaagagaaa ttcggcttcc tcttggacaa cctgaaattc ggtgcgcctc ctcacggcgg
     6121 ccttgcattc ggcctcgacc gtctggtaac gctgatgacc ggagccgaat ccatccgcga
     6181 cgtgattgcc ttcccgaaaa cacaacgcgc acaatgcctg ctgaccaacg cgcccaacag
     6241 cgtggacgac aagcagttgc gtgaattgag cctgcgcctg cgccaaaagg tggctgaaac
     6301 caaagaagca taaggaaaac agagccgttt gacggctctg tttttttcag acggcattta
     6361 cgcttcttga cttccctcta attcaaacct aattttgctg tgttttaatg gcggtattga
     6421 aaaacacatc ttgttcaatc aaccgataaa aaaaggactg aaaatgaaaa aactgttatt
     6481 ggccgccgtt gtttccctga gtgccgcaac cgcatttgcc ggcgattctg ccgagcgtca
     6541 gatttacggc gatccccatt ttgaacaaaa ccgcacaaaa gccgtgaaaa tgttggaaca
     6601 gcgtggttat caggttcacg atgtcgatgc cgacgaccat tggggtaagc ctgtgctgga
     6661 agtggaagcc tataaagacg gccgcgaata cgacatcgtg ttgtcttacc ccgacctgaa
     6721 aatcatcaaa gagcagctcg atcgctgact cctttgatgg caagctgaac caaaatgccg
     6781 tctgaagcgt tcagacggca ttttgcctgt tcctcatcag gtatgaggca ggcttttctt
     6841 attaaaaaaa tgacatttca cgctgatttg ttataatcat tccttttcaa cacgacagac
     6901 ggagcaggtt tattatgcct atccttacca tccgtgaagt gtgcaacatt aatcattggg
     6961 gcataggtta ttatgatgtt gacgattccg gcgaaatcat cgtccgcccc aatccggcgc
     7021 aacacaatca aactgcttca ctgcaaaaac tgactgaagc cgtgcaacaa aaacatcagg
     7081 cgcgcctgcc tgttttgttt tgttttccgc aaatcctcga acaccgcctc cgcgacatta
     7141 accgcgcctt tcagacggca cgggaagagt gcggctataa gggcggttat tgtttggttt
     7201 accctatcaa ggtcaaccaa caccgccgcg tcatcgaatc gcttatgtca agcggacaac
     7261 cgcatggttt ggaagctggt tctaaagccg aactgatggc ggttttggca cacgccggca
     7321 cccggcaaac attaatcgtc tgcaacggct ataaagaccg tgaatatatc cgtttcgcct
     7381 tgatgggcga aaaactgggg catcaggttt atttggtgat tgagaagctg tccgaaatac
     7441 aaatggtatt ggaagaggcg gaaaaactcg gcatcaagcc ccgtttgggt gtgcgcgcca
     7501 gactggcttc ccaaggttcg ggaaaatggc agtcttcggg tggggaaaaa tcaaaattcg
     7561 gcttgtcggc ttcccaagtt ttgcaactgg tcgatatttt gaaacaaaaa aacaggctgg
     7621 attgcctgca gcttttgcat ttccatttgg gctcgcagct tgggaacatc cgtgatgttg
     7681 ccacaggtgt acacgaatcg gctcggtttt atgttgagtt gcacaaactg ggggtaaata
     7741 tccgctgttt tgatgtaggc ggcgggcttg gcgtggatta cgaaggaaac cgcacacaat
     7801 cggattgttc cgttaattac agcctcaacg aatatgccgc cacagtcgta tggggcatca
     7861 gtcaggcttg tctcgaacac gggctgccgc atccgacaat catcaccgag agcgggcgcg
     7921 gcattaccgc acatcacgcc gttttggttg ctaatgttat aggcgttgag cgttacaaac
     7981 cgcgccggct ggatgcgcca tcgcccgaag cgccgcgtgt gttgcacagt atgtgggaaa
     8041 cttggacgga tatttccgcc tcgcgggaaa aacgttcctt acgcagctgg atacacgaag
     8101 ggcagtttga tcttgccgat gtgcataatc agtataatgt cgggctgttg agtttggcgc
     8161 aacgtgcgtg ggcggagcaa ctgtatttaa atatctgtca tgaagtcggc gaattgttta
     8221 atgaaaaaca ccggtctcac cgaaccatta ttgacgaatt gcaagaacgt tttgccgata
     8281 agctgtatgt caatttctca ctcttccaat ctttgcccga tgcttggggc atagatcaac
     8341 ttttccctgt ttgtcccatt accggtttga atgaaccgat tgcgcgccgc gccgtgttgt
     8401 tggacattac ctgcgattca gacggtacga ttgaccacta catcgacggc gacggcatcg
     8461 ccggtacgat gcctatgcct gattatcccg aagaagagcc gccgctttta ggctttttta
     8521 tggtgggagc atatcaggaa atactcggca atatgcacaa tcttttcggc gacactgcca
     8581 ctgccgatgt tgttgtaggg gaagacggac aatttaccgt catcgattac gatgaaggaa
     8641 acaccgttgc cgatatgctc gaatacgttt atcaagatcc gaaagagctg atgaaacgct
     8701 atcgcgaaca aatcgaacat tcagaccttc ctgcctcgca ggctatgtct ttcttaaaag
     8761 aactcgaagc ggggcttaat ggttatacct atttggaaga cgaatagacg catcaaggca
     8821 tcggatatgc cgtctgaagc ccgattttct tcctcaaaca ccaatcacca cgaccgattg
     8881 aaaccaatta caaggaatca ttacgatgca atacagcaca ctggcaggac aaaccgacaa
     8941 ctccctcgtt tccaataatt tcgggttttt gcgcctgccg cttaatttta tgccgtatga
     9001 aagccaagca gattgggtca ttaccggcgt gccttatgat atggcggttt cagggcgttc
     9061 cggcgcgcgt ttcggtcctg aagccatccg gcgcgcctcc gtcaacctcg cttgggagca
     9121 ccgcaggttt ccatggacat ttgatgtgcg cgaacgcctg aacattattg attgcggcga
     9181 cttggttttt tcttttggcg acagcaggga ttttgtcgaa aaaatggaag cgcacgccgg
     9241 caaattactt tcttccggca aacgctgttt gagtttgggc ggcgaccatt tcattaccct
     9301 cccgttgttg cgcgcccacg cccgctattt cggcaaactc gcactgattc attttgacgc
     9361 gcacaccgac acctacgaca acggcagcga atacgaccac ggtacgatgt tctataccgc
     9421 ccccaaggaa ggcctcatcg acccgtcccg ttccgtacaa atcggcatac gcaccgaaca
     9481 cagtaaaaaa ttgcctttta ctgtgttgtc cgcccctaaa gtcaatgaag acagtgttga
     9541 agagaccgtc cgtaaaatca aagaaaccgt cggcaatatg cccgtttacc tgactttcga
     9601 catcgactgc ctggacccgt cgttcgcccc tgggaccggt acgcccgtat gcggcggctt
     9661 gagcagcgac agggcattaa aaatcctacg tgggctgacg gatctcgaca tcgtcggtat
     9721 ggatgttgta gaagttgccc cctcttacga ccaatccgac attaccgctt tggccggcgc
     9781 cacaattgcc ttggaaatgc tttaccttca aggtgcgaaa aaggactgaa cgtccggcat
     9841 cccccgggtt ttcgccgtgc cgttcaaacg gcgtattcag tctaatgaaa attcaaatac
     9901 tgaaacaaaa gttgcccgga gccgcatatc ggaaagacgg tgaaatatcg gaatatatct
     9961 tataaaacaa ttagttaaat attatttttc cgatttttcg ggacggtctt ttttacggag
    10021 gtcaatatga tgaaattggg tttcaaaccg atacccctcg ccattgccgc agtattgtgc
    10081 gccctggttt tggcactgcc cgtacccgac ggggtcaagc ctcaggcttg gacgctgctg
    10141 gccatgtttg tcggtgtgat tgccgccatt atcggcaagg ccatgccgtt gggcgcgctg
    10201 tcgattattg ccgtcgggtt ggtcgcagta accggcgtaa ccgccgacaa accgggcgcg
    10261 gcgatgagcg atgcgttgag tgcgttcgcc aatccgttga tttggctgat tgccatcgca
    10321 gttatgattt cgcgcggttt gctcaaaaca gggctgggga tgcgtatcgg atatttgttt
    10381 atcgccgttt ttggaagaaa aacgctgggc atcggttaca gtctcgctct ttccgaactg
    10441 ctgctggctc ccgttacccc ttccaatacc gcgcgcggcg gcggcattat acatccgatt
    10501 atgcagtcga ttgccggcag ttacggctcc aatcccgcaa aaggcacaga aggcaagatg
    10561 ggtaaatatt tggctttggt caactatcat tccaatccca tttcgtcggc tatgtttatt
    10621 actgcaactg cccccaaccc tttaatcgtc aacttgattg ccgaaaattt aggcagtagt
    10681 ttccgtcttt cttggggggc gtgggcgtgg gcaatggctg ttcccggcgt tatcgccttt
    10741 ttcgttatgc ctttgatttt atattttttg tatccgcctg aaattaaaga aacgcccaat
    10801 gccgttcaat ttgccaaaga ccgtctgagg gagatgggta aaatgtcggc agacgaaatc
    10861 attatggcgg tcattttcgg tatcttgttg ctgttgtggg cagatgttcc cgcccttatt
    10921 accggcaatc acgcttttag tatcaacgcc accgccaccg catttatcgg attaagcctg
    10981 cttttgcttt ccggtgtatt gacttgggac gatgttttga aagaaaaaag tgcgtgggat
    11041 acgattattt ggtttggcgc attgattatg atggccgcat ttttaaataa actcggactg
    11101 attaaatggt tctccggagt gttggcggaa agtgtcggcg gtttgggcgt tagcggcacg
    11161 gctgcgggcg taatcctcgt gcttgcttat atgtatgcgc attatatgtt tgccagtact
    11221 actgcacata ttaccgctat gttcggcgca tttctcgctg ctgccgtttc actgaatgcc
    11281 cctgcgatgc cgaccgcgct gatgatggcg gccgcatcca acattatgat gaccctcact
    11341 cattatgcga ccggtacttc gcctgtgatt ttcggttcgg gctacaccac aatgggagaa
    11401 tggtggaagg cgggttttat catgagcgta gtcaattttc tgattttttt cgttatcggc
    11461 agcatttggt ggaaagttct ggggtattgg taagggaaaa ataaaataaa tttccaatct
    11521 gtgtttattt gattgggcga ctattatcgt gaaatatgcc gtctaaagcc ttcagatggc
    11581 atatttgtgc gcttgaatgt tgcagaaagc ggcaggcggc ggtgtaggaa aagccaaaca
    11641 aaaaccaaac cgcctatcaa cttctgataa acataagcat taaataatca gaaggttatt
    11701 caattaccta aacgcaaatt tcccttacct aaacgcaaat ttccctgccg tatcacatct
    11761 attgaaaata atacatcaac cggctcggaa gcagcctgat caggtgtttc tacttgcggc
    11821 gatgaatcgg cagccggttc ggtataggca gtcggcgtgc cgtcggattg ggtatttaaa
    11881 tgcggttgtg cctcgggcgt gtgtacatca ggcacttcgg gcgtgcgtgt ctcggatatt
    11941 tcggcagagt tggtttcctc agtttgttca atgacttcag cttggctgta tgaggaagaa
    12001 ccctgtatcc acgccagcga tttgagcggc atcttcatct tgccgttttt gccgcaggtc
    12061 aggcagacgg ccgatccggt gcggtctttg agtacagcat cgactttctc gggcgcgatg
    12121 gttttaccct gtgtttttgc ccattgcgca atactttttt tcagagaggc gatgtcaagg
    12181 ttgttcttac cgtaagggtc gcggaaggcg gcaagccaca ggttgcgatc cgcatcttca
    12241 ttgagacccg ccatttgata gatgacggag gcgggcgagc cgctggcgat ggttttggca
    12301 aactcgagcg cgtcgggcga tttcatgcgg acgcagccgt gactccgaac cccggggacg
    12361 ctggccggcg cattggtccc gtgtatgccc aaaccgagtt tagggtcgcc taagcggaca
    12421 aaaaccggcc ccaaagggtt gtccgggccg gcggctatgg tttttacgcc gtcgccgcgt
    12481 tctttctgta tggatttggg gatgtaccaa acagggttat aggctttcgc accgatttta
    12541 tgttcgccta gattggtttg cgtcatcgcc cgacctactg caacgggata aaccttggtc
    12601 agtttgccgt cggtgtagag gaacaggcgt tgctgaggga tgttaatgaa gacatgttga
    12661 ccttgtgcga cgggggagac atcgggaatg acggtgtttg cgtatgaaaa accgcttatc
    12721 aatagtgcag cagtgcggca gattgtttta ttcatatcaa aatatggtgt gtgtccgata
    12781 ggttttcggc aaatcatacc tgaaaccgta ccaatttgtg cgaaaatatg cgcttcggta
    12841 cagtgcggac ggattgggta atggcaacgg aaacaaatgt cgcggaaatt tccgccttgg
    12901 attatgaagg caggggtgtg gcaaaggtcg gcggcaaaac ggtttttatt aaaagggcat
    12961 tacttgattg tttgatgctg ggttggttca ggctttaact caggaatatt tacatcataa
    13021 tgaaggtttt taaacaacag cttgaacaac tcggcgcgca aaaccaatat cgttcgattc
    13081 cggatttgat tcatcaaggg cggtatatta cgcgggaaaa ccgcaaaatg ctgaatatgt
    13141 cgtctaatga ttatttgggt ttggcatcag atgaaaactt gcgccggtct tttttgcagc
    13201 aatacggcgg taattttccc tcttttacca gttcttcatc gcgtttatta acgggcaact
    13261 ttcctattta taccgatttg gaagagcttg tcgcacaacg tttccaacgg gaaagcgcgt
    13321 tattgttcaa cagcggctat cacgccaatc tcggtatttt gcctgctttg acgacgacga
    13381 aaagtttgat tttggcagat aaatttgttc acgccagtat gattgacggc atccgtttga
    13441 gccggtgtgc gtttttccgt tatcgtcata atgattatga acatttgaaa aatctgcttg
    13501 aaaaaaacgt cggaaaattt gaccgcactt ttatcgttac cgaatctgtt ttcagtatgg
    13561 acggcgatgt ggcggatttg aaacagcttg tccaattaaa aaaacagttt cccaatactt
    13621 atctttatgt ggatgaagcc cacgcaatcg gtgtttatgg gcaaaacgga ttggggattg
    13681 ccgaacggga taatttgatt gccgagattg atttactggt cggcactttc ggtaaagcct
    13741 tagcctcggt gggggcgtat gccgtctgca accaagtatt gaaagaatgt ttgattaatc
    13801 aaatgcgtcc attgattttt tcaaccgcat tgccgccgtt taatgtggct tggacttatt
    13861 ttatttttga acgattgccg caattctcaa aagaaagaag ccatcttgag cagttaagcg
    13921 catttttacg gcgggaagtg gcgcatcgga cgcaaataat gccgagccaa acctgtatcg
    13981 tcccctatat tttaggcggg aatgaagccg cccttgccaa agcggaatat ctgcaaaggc
    14041 agggttatta ttgcctgccc atcagaccgc cgacagtacc caaaaacaca tccagaatcc
    14101 gcctgtcttt aacggcagat atgacaacgg atgaagtgcg gcagtttgcg gtatacctgt
    14161 aaggatatga tatgaaaaca aaattttaca atcatcaagg cggacattta atcctgtatt
    14221 ttgcaggttg gggaacgccg cccgatgctg taaatcattt gattttgccg gaaaatcacg
    14281 atttattgat ttgctatgat tatcaagatt taaatttgga ttttgatttt tccgcctatc
    14341 ggcacatccg cttggtggcg tggtcaatgg gcgtttgggc ggcagagagg gcattgcaag
    14401 gaataagatt aaaatccgca acggcagtga atggcacagg tttgccttgc gatgataatt
    14461 tcggtatccc ttgcgccgtt tttaaaggca cattggagaa cctcacggaa aatacccgtt
    14521 taaaatttga acgcagaatg tgtggcgata aagcatcttt tgaagattac caacaatttt
    14581 ccgcccgtcc gtttgacgaa attcatcaag aacttatcgc actttttgcg atgatcgggc
    14641 aagataggcg cgcagatttt atccgctgga caaatgcctt ggtcggatcg ggcgataaaa
    14701 tttttatgcc tgccaatcag caccgatatt ggacaccgcg ttgcaccgtt cgggaaattg
    14761 acgtcggaca ttacctgttt tcaagattca cccattggtc ggcactatgg aatcactgac
    14821 tgccataaat aaatcgcgca ttcggcaggc tttccaaaaa gcattaaacg attatgaccg
    14881 gcacgcctta atccaacaaa aaatgacgat taatttaatg acgcatttgc aagattattt
    14941 gccggatatg ccattggaaa acgtgttgga attgggctgc ggctcaggaa tgttgagtgc
    15001 cttgctgcaa aaacagattt cagcgaatta ttggttattt aatgatttgt gcaatgtgca
    15061 gccccaactg gctgaaaaac tgccgcaatc ctttgatttt tattgcggcg atgcggaaaa
    15121 ctttcctttt caacgacaat ttgacttaat cgcaagcgca tctgccgtgc aatggtttca
    15181 tcaacccgac gcttttatca cccattgcaa aacaggcttg aaaacaaacg gattattggc
    15241 ggttgcaacc tttggcaaag acaatttaaa agaagtccgc caaattacaa atataggctt
    15301 aaattacccg actttatccc aatggcaggc ttggttagcc aaagattttg agcttttatg
    15361 gtgtgaggat tttacggtaa tactagactt tgatacgccg tcagatgtac tcaaacacct
    15421 taaatataca ggcgtaacag ccacgaacca aaaaaattgg acaagaaaaa atctcaatgg
    15481 atttattggc gattacttgt cggcgttcgg tatgccgtcg ggcaaagtgc gcctgactta
    15541 tcatccgtta ttttttatcg cgcgctactc tgcggcgggc aggcaataag gcagcttatg
    15601 ggcaaagtta tttttatatc gggtattgat actgatgtgg gtaaaaggta atatggcgag
    15661 gcttgtgcag aaggcatatt gttaaacgtt aaattatggt atgatttaaa acttacaagt
    15721 ctatttcagt aaatcgttaa taataaaagc ggacaatggc cgttgcaggc ggtcagctat
    15781 ccgctcgtta cggtatcggg agtatccttt ggaaaactca ttaattatcg gtttggcact
    15841 ggcggtattg ctgatgcttt tggttatgcg tgcgaagcag ggtaagaaaa caccgaagcg
    15901 caaatcccaa ggtgtcggca atacgcaggg gcagacaccc ggaagcaatg attcggactg
    15961 ggtcgatcag attggacaat cggtttcaga cggcacgcaa cccgactggt cttggaacga
    16021 aagtgccgag accgcatccg ccgccgtatc cgcgcaagaa gtcgatccgc ttacggagta
    16081 tcaggtttat aagcaattcg gttatcaggg caaggctgcc gaatctttgg ctgcctatct
    16141 ggacggcatt ccggatggtg aagcgaaacc tgaaaacctt atccgcgagc tgctcgatat
    16201 caatctcgaa gtgggggatg tcgatgtttt ggcagacaat ctgcaaaaat acggcaaact
    16261 gattccttcc gaacttttgg caaaatatat cgaacaggca ttacagcgcg attcaaacca
    16321 tttgcgtatc cgcgtcttgg cggaagaagg tttgggatgg ggtactcagg agattgaaaa
    16381 acgtgcggaa ggcggttctg cgacggcagc ttccgcatcg cccccgccgg atgccggcgg
    16441 taaggcttat gaagccgaag aaatcaagcg catcccgatt gtgcggggca aaaaagacgt
    16501 gtccggaatc agtcaagagg aaatcggtgc gattgccggt ttggtccgtg ccgatcaagg
    16561 tgcgaaaatc cttaaagaca aagtcagcta tgaaacggca tcgaaacaat acgaccgtgc
    16621 catccaaact tccgaaaaac ctgcaaacct gattatcgat gcgttgaaac tcgattacca
    16681 atacgcggac atagaccgtt ttgccggaca tttgtggaaa ctttaccaaa cgttgggcaa
    16741 ctacggcagg caggttaaag agcggatgct ggggtggggg tacagcctgg gttaccatga
    16801 ggttttcgat gatttggaaa aagggccgaa cgaccggcaa atcaaagaca tcggtatggg
    16861 gcacgggtat ctgccgaaaa atatacagaa attcaaatcg caacatcggg atttggtgct
    16921 tcaagattct tcgttgatca acaccggttc gtctccggca gacgatgcgg ttaaggaagt
    16981 agagtcgttg ctgatgtatg gtcagattga agcggcaatg gatgtgttgg agcaggcggt
    17041 attgaaatat cccgacgagt cccagcttta tattacgttg atcgatattt atgaacgtac
    17101 tgaagattgg gataggttgg ggcagttttt aagggtattg agggaacgtg cggacaggct
    17161 tcctgaagag gtcgttatgc tgatgagccg gctgctgcag cgtatgaatc aaaatattaa
    17221 aaaaataaaa cggtacggaa aataaaaatg gaagttcaac tgccgaaaat taaaacagta
    17281 cgcgtaatgt tggcggggat gacggcgcag caggaatccg ttttcaaaat ggcattcaaa
    17341 atgcacaata ccacccgtta tgaaacagta tccccttcag acggcagtgc cgtgcccgat
    17401 ttggttttgg cggataccga tgccgagggc ggttttgaac tttggaaaga gcttgccgag
    17461 cgttataagg atatacccgt cgccgtctgt tcggagaaag ttcccgattc tgaagttccc
    17521 tacctgccca aaccgattcg gtttgaaaca ttgtttccta tgctccgcaa attgttgcag
    17581 ggcgagaatg tttatgggaa atcgtttatt gcacccgcag accggtcggc gaaaaataac
    17641 gggaatgtgc agcgtacggt tacgatacgc cagtttaacc cgaataaagg attattgggg
    17701 gcgttgcggt ttgcggaaaa gaacaggcag gacatcgcta tcttgcatgg aaataagccg
    17761 gtccttattg ttttcccctc gatacaacgg gttttgctga cagaaagtgt gcaaaaactc
    17821 gaagaattgt gcaaagacga aaatttgcag gtcagctgca agactgttcc cgataacccg
    17881 caatggcgcg aaaaggctaa agtaggcatt atgtcctgta tgtggcagtt ttccatttgg
    17941 acggcgcagg gcaggttgat ttatccgatt tctcccgata ctccgtttac gttgaaatct
    18001 tggccaaacc tgacccggtt ggcaaatgtg ccggggtcga tacgcttgtc ggcatttctg
    18061 accaaggcat ccgtcaacct taacgtgttg tataaagtga tgcctttaaa cctcaatgat
    18121 attctgaatt atcttgcggt aacctataca accgggtttt tgtcggtaga tttaaaaacg
    18181 gtttcacaac aggcatactc cgatatggcg gataaaataa atatcggagc cgattctgcc
    18241 tctgatagtg aaatgatgaa aaaagcggaa aaaatcacaa caccatccca atcccagtcg
    18301 cgcggtcttc tgcaaaggct gatgaaaaaa ctgttgggca gctaagaggc ggagagatga
    18361 gagaaaataa aattattttc acaggacctg tcggcgtagg gaaaaccact gccattgcgg
    18421 ctatttcgga cgaagcactc gttcagaccg atgcttccgc atccgatatg actttggata
    18481 ggaaaaggaa tacgacagtg gcgatggact acggggccat cagcttggat gaggatacca
    18541 aagtccattt atatggtacg cccggtcagg aacggttcaa ctttatgtgg gaaatcttaa
    18601 gccaaggcag tatgggtttg gtcttgcttt tagataatgc ccgaaccaat ccgttgaaag
    18661 atttggaatt ctttttacat tcgtttcgag ggctgctgga gaaggcaccc gtcgttgtcg
    18721 gtattaccaa gatggatata cgctctcagc ccggtatcga cgtgtatcac aaatatcttg
    18781 caaaacataa tcttaatgtt ccggtttttg aaattgatgc ccgtaaggaa gatgacgtaa
    18841 aacaattggt tagcgcaatg ttattttcta ttgatccggg actggaggtt taatatggaa
    18901 tcaacacttt cactacaagc aaatttatat ccccgcctga ctcctgccgg tgcattttat
    18961 gccgtatcca gcgatgcccc cagtgccggt aaaactttgt tgcacagcct gttgaaagcg
    19021 gatgcggacg aaatggtcag cagtgagaag ctgcttactt gggcggacac cgccgacatc
    19081 gataccgctt tgaacctgtt gtaccgtttg caaaaactcg aattcctcta tggcgatgaa
    19141 aacggtcatt cagacggcat caatttgtcg gacgagcaat tgccgttgct gatggaacaa
    19201 ttgtccggca gcggtaaggc gttattggtc gatcggaacg gtctgtatct tgccaacgcc
    19261 aatttccatc atgaggcggc ggaagagttg gggttgttgg cggcagaagt cgcacagatg
    19321 gaaaagaaat accggctgct gattaagaac aacctgtata tcaacaataa cgcttggggc
    19381 gtttgcgatc cttccggtca gagcgaattg acatttttcc cattgtatat cggttcaacc
    19441 aaatttattt tggttatcgg cggcattccc gatttgggca aagaggcatt tgttactttg
    19501 gtaaggattt tataccgccg ttacagcaac cgcgtgtaaa acttgggaga gaggaggggt
    19561 tatgcagcaa ttattgattt caatccttga agatttaaac aatacatcta cggatattat
    19621 cgcgtctgcc gttatctcaa ccgacggatt gccgatggcg acaatgcttc cttcacattt
    19681 gaattcggac agggtagggg cgatttctgc cactttgctt gctttgggga gtcgctcggt
    19741 gcaggaactc gcctgcgggg aattggaaca agtgatgatt aaaggaaaat caggctatat
    19801 ccttttaagt caggcgggta aagatgccgt gttggtgctg gtggcaaaag aaaccggcag
    19861 acttggttta atcctattgg atgccaaacg tgcggcaagg catattgcgg aagccatata
    19921 acatataaag attgcgggct tgcagataaa gtgcaatcga ttgtcaattt atattgacac
    19981 gttcggtatt tctgttttat tattcgcgct tattccccga tagctcagtc ggtagagcga
    20041 cggactgtta atccgcaggt ccctggttcg agcccaggtc ggggagccaa atttcaaaac
    20101 cctctaagta ttttcttaga gggttttgtt ttaccggcgg tcagaaacgc atttttgaga
    20161 tgattgtttt gagatggaat aaaatctttg caaaattcct ttcgtgatgg ttatgaaaaa
    20221 ataggggctg tcctggacaa ctaggataaa ctcgatttta tagtggatta acaaaaacca
    20281 gtacggcatt ggctcgcctt
//
LOCUS       DAWKOD010000021        19643 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000021 DAWKOD010000000
VERSION     DAWKOD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.021"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(49..2886)
                     /locus_tag="WJK73_000556"
     CDS             complement(49..2886)
                     /locus_tag="WJK73_000556"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ7557078.1"
                     /translation="MLDKYNPAEIESKHYQNWESQGYFRPDMDLAKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVCLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIVDEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHGTRLVNVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSFADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADSLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLGKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WJK73_000557"
     CDS             complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WJK73_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ7557079.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(3973..5229)
                     /locus_tag="WJK73_000558"
     CDS             complement(3973..5229)
                     /locus_tag="WJK73_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ7557080.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGQFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFNNRIRVGGMAELSGYAIKLPEKRRETLAL
                     VVNDLFPEGGDLNQTLFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5308..6699)
                     /locus_tag="WJK73_000559"
     CDS             complement(5308..6699)
                     /locus_tag="WJK73_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7557081.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPTYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WJK73_000560"
     CDS             complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WJK73_000560"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ7557082.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VISGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(7875..8555)
                     /locus_tag="WJK73_000561"
     CDS             complement(7875..8555)
                     /locus_tag="WJK73_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ7557083.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8762..9211)
                     /gene="fabZ"
                     /locus_tag="WJK73_000562"
     CDS             complement(8762..9211)
                     /gene="fabZ"
                     /locus_tag="WJK73_000562"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ7557084.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFESMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(9247..10290)
                     /gene="lpxD"
                     /locus_tag="WJK73_000563"
     CDS             complement(9247..10290)
                     /gene="lpxD"
                     /locus_tag="WJK73_000563"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ7557085.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPDATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(10323..10823)
                     /locus_tag="WJK73_000564"
     CDS             complement(10323..10823)
                     /locus_tag="WJK73_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ7557086.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10889..13282)
                     /gene="bamA"
                     /locus_tag="WJK73_000565"
     CDS             complement(10889..13282)
                     /gene="bamA"
                     /locus_tag="WJK73_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ7557087.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(13339..14679)
                     /gene="rseP"
                     /locus_tag="WJK73_000566"
     CDS             complement(13339..14679)
                     /gene="rseP"
                     /locus_tag="WJK73_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ7557088.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(14714..15949)
                     /locus_tag="WJK73_000567"
     CDS             complement(14714..15949)
                     /locus_tag="WJK73_000567"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ7557089.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15954..16751)
                     /locus_tag="WJK73_000568"
     CDS             complement(15954..16751)
                     /locus_tag="WJK73_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ7557090.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(16754..17500)
                     /locus_tag="WJK73_000569"
     CDS             complement(16754..17500)
                     /locus_tag="WJK73_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ7557091.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITADTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(17556..18113)
                     /gene="frr"
                     /locus_tag="WJK73_000570"
     CDS             complement(17556..18113)
                     /gene="frr"
                     /locus_tag="WJK73_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ7557092.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(18294..19421)
                     /locus_tag="WJK73_000571"
     CDS             complement(18294..19421)
                     /locus_tag="WJK73_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ7557093.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(19491..19566)
                     /locus_tag="WJK73_000572"
     tRNA            complement(19491..19566)
                     /locus_tag="WJK73_000572"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19531..19533),aa:Thr,seq:cgt)
BASE COUNT         4045 a         5037 c         5716 g         4845 t
ORIGIN      
        1 aggccgtctg aaaaacattt ttcagacggc cttgttgatt caatcaaatc agtctttcaa
       61 cttcgacaac tgagtttgca cttttgccat tttgtcttcc aattccgcca aatcggcttt
      121 gtctttttcc accaaatgcg caggggcttt ttcggtgtag ccgggtttgg agagtttggc
      181 gttgagtttg tccaaggctt tttgcagctt ttcggcttct ttgcccaaac gggcggtttc
      241 ggcggctttg tcgatttcga ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg
      301 cgcgtcttcg ctttcgggta gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat
      361 cggcaggtat ttgagggagt ccgccaagtc gtccgcgctt tcgacaaaca gcggggcttt
      421 cacgttgggc tggatgccca tttcgccgcg caggttgcgg actgcgccaa tcaaatcctg
      481 caacacggtc atttgctcga atgccgtctg aacaatttcg ccgccgtcgg cttcggggaa
      541 gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac ataggggcga cggtttgcca
      601 cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac
      661 gcgcaacaag gtatggcgtg tggcgcgctg gcggctggcg caaccggttt gaagctgcac
      721 tttggcgagt tccaaatacc agtcgcaata gtcgttccac acgaagctgt acagggtttc
      781 cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgttcga tggtctgatt
      841 cagacggcct acaatccaca tatcggggaa ggaatagccg cgcggttcgg cagcggttgc
      901 gccgtagccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg cgttccagat
      961 tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttga cgctgcggcc
     1021 gaggctggcg tagctcgcca tagtgaagcg caatgcgtcc gcgcccatac tcggaatgcc
     1081 ttcggggaag agttttttgg tggcttcttc cactttcggc gcggtttcgg gtttgcgcag
     1141 accggtcgtg cgtttcacca gcagcttgtc caagtcgatg ccgtcaatca aatcgacagg
     1201 gtcgatgacg ttgccttctg atttggacat ttttttgcct tcgtggtcgc gcacgatgcc
     1261 gtggatatac acgtctttaa acggtacttt gccggtaaag tgggtggtca tcataatcat
     1321 gcgcgccacc cagaagaaga tgatttcgta gccggtgacc aagacgttgg acggcaggaa
     1381 ggctttgagt tcgtcggttt cagacggcca gccgagcgtg gagaacggca cgagtgcgga
     1441 ggagaaccat gtgtccaata cgtcttcttc tcgggtcaag cctgttttgc cggcttgttt
     1501 ttcggcttct tcctgattgc gggcaacgta cacattgcct tcgttgtcgt accatgcagg
     1561 gatttgatgc ccccaccaca gttggcgcga gatgcaccag tcttggatgt tgttcatcca
     1621 ttggttgtag gtgttgaccc agttttcggg gataaagcgc accgcgccgc tgtcgacggc
     1681 ttttttggct ttgtcagcaa agcttaagcc tttgaattcg ctgtcaggct cgccgccgtt
     1741 cggtgtggcg gacatggcga caaaccattg gctggtcagc atcggctcaa tcaccgaacc
     1801 ggtacggtcg cctttcggcg tcatcagcgt gtgcgctttg atttcgacca agaaaccttg
     1861 ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtccaaac ctgcgtattt
     1921 ttcaggcagg gcaaagcctt gttgcgcttc acctttgaag ttgaacactt cggcgtttgc
     1981 cagcactttg gcttccaagt cgaacacatt aaccaagcgc gtaccgtggc gtttgccgac
     2041 ttcgtagtcg ttgaagtcat gtgcaggcgt gattttcacg cagcctgtgc cgaaatcttt
     2101 ttcaacgtat tcgtcgacaa tcacgggaat ggtacgaccg gtcagcggca ggattaattc
     2161 tttgccgatt aagtgggtat aacgttcgtc ttcgggattg acggcaacgg caacgtcgcc
     2221 caacagcgtt tcgggacggg tggtggcgac gatgacggct tcggcaggat tgtccgccag
     2281 cggatagcgg atgtgccaca tagagccttg ttcttccacg ctttccactt ccaaatccga
     2341 cactgccgta cccaaaaccg gatcccagtt caccaagcgt ttgccacggt aaatcaagcc
     2401 ctgttcatac aggcacacaa acacttcggt tacggtttca gcgcgcacgt cgtccatcgt
     2461 gaaatactcg cgcgtccagt cggcggaaca gcccacgcgg cgcatctgct gcgtaatcgt
     2521 gccgccggaa acttctttcc attcccacac tttttccaag aatttttcgc ggcccaagtc
     2581 atgacgggac acgttttgcg cggcaagctg acgctcgacc acgatttgtg tggcgatgcc
     2641 cgcgtggtcg gtgccgggaa tccaggcggt gttgcagcct ttcatgcggt agtagcgggt
     2701 caggccgtcc ataatggttt ggttgaaggc gtgccccata tgcaatgtgc cggttacatt
     2761 gggcggcggc agttggatgg aaaaagacgg tttggccaaa tccatatcgg gccggaaata
     2821 accttggctt tcccagtttt gataatgttt ggattcgatt tcggctggat tgtatttgtc
     2881 taacatgatg gaactttgtg aaattaaggt tatttttgat gtgcggatta taacgcaaaa
     2941 aggccgtctg aatcatttca gacggccttt ggcatacggg ttttaaaaat ggaacaatac
     3001 caggctgacg gcaatcaccg ccatgcccat tgtcaggccg taaacggttt catggccgtc
     3061 tgaatagcgt ttggcagccg gcagcagctc gtccaacgcc aaaaacacca tcacaccggc
     3121 tatcacgccg aataccgaac caaacacggc aggcgacaaa aacggctgca aaaccaaata
     3181 gcccaaagcc gcccccaacg gctcggccaa gccggatagc agacacgccc acaccgtttt
     3241 cttacggctg cgggtggcaa aataaaccgg cgcggcgatg gaaatgccct ccggaatatt
     3301 atggatggca atcgccaagg ccaaaggcat cccgactgct ggattttcca atgtggcaaa
     3361 aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc
     3421 gactcgcgcg atatggcggc gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt
     3481 ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg ccgcccatcc cggccaaaaa
     3541 tgccatggtc gccgccgcaa acgcgtggtc tttatcataa atttcagcga acgcctcgct
     3601 ggacttactg aaaatctccg tcagggaaac atataccatc gcaccgccgg caaacgccaa
     3661 accaaacgac aacacacgcg gattgggcgt tttggaaaac atcaccaagc cactgcctaa
     3721 tacggtaaac aaaccggcag ccaatgtgat ggaaaaggca acggccaaat tggacatcga
     3781 aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca ggttcagact aacacttttt
     3841 aatgtatatg ataatagtta ttatttattt tattgattgg atacacggat tttgaaacaa
     3901 aagaccgtct gaaaaatgat tttcagacgg cctttaaatt tgaaatgccg ctaaacctta
     3961 gtgctttcca gcttaagcct gataacgcga caggctcaaa tcgtcgctgc ggatttcggt
     4021 gtctttgccg ctcacgatat cggcggttaa ttttgccgaa cccagcgaca tggtccagcc
     4081 taaagtaccg tggccggtat tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg
     4141 cgtgctgtcg ggcgtcatcg gtctgagtcc gctccagaac aatgtttggt ttaaatcgcc
     4201 gccttcgggg aacaagtcgt tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag
     4261 tttgattgcg tagccggaca attccgccat gccgccgacg cggattctgt tgttgaagcg
     4321 cgtgatggcg actttgtagc tctcatctaa aacggtggac accggtgcgc cgtcggaatt
     4381 tgtgaccggc agagtcaagg aatagccttt gacgggataa atcggcagat tgagatccaa
     4441 ctgtgccaaa accgtcctgc taaagcagcc gagtgcgcag acaacggcat ctgcttcaaa
     4501 ctgccctgtt tcggtttcaa cggttttgat gcgcagcccg ttgtggtcga tgcggctgat
     4561 ggtttggttg aaatgaaacc gtacgccctt ttcctgacac aatttgtaca ggttttcagt
     4621 gaagaggcgg cagtcgccgg tcgcgtctgc gggcaggtgc aggccgccgg caattttggc
     4681 ggtaacgcgt gccagcgcag gctcgaattc tgcgcattct tcgggcttca gacggcggta
     4741 cggcacgccg tagcgttcca aaacggcaat gtcttgtttt gccgcttcga cttctttggt
     4801 ttggcggaaa atctgcaacg tccctttttt gcgtccctca aaattcatgc cggtttgcgc
     4861 ttcaaaacgg cggaacattt cacggctgta ttcggacatc ctgaccatgc gctctttatt
     4921 gatttgatag cgggatgccg tgcagttttg cagcatccgc cacagccatt cgatttgata
     4981 caggctgccg tcggggcgaa acagcaaagg cggatggctt ttaaacagcc atttcagcgc
     5041 tttggtcgga ataccgggtg cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc
     5101 gttggcaaaa ctggtttcca tcgccacgcc ctcggcgcgg tcgatgaccg ttacttcatg
     5161 tcctgcctct gccagatacc acgcggaaga cacgccggca acacccgcac ctaaaacaag
     5221 cactttcatg tttctccctc cggctttttc aaaacagact taatatgccg tgccgtctga
     5281 atattcggat tcagacggcc tcggatatta atgcggcaat tcgccgtttg tgattttttg
     5341 tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc aataaaagtg caatcaggtt
     5401 gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa
     5461 cacggtaccc agcataacgg aagaaacata acccacgcgg tacaaaccgg caaatttctc
     5521 gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac cagcccaaaa tggttgagta
     5581 ggcaaagaaa atcaggccga tggtaacaat ccagccgccg atgccgggca gcattttttg
     5641 gaatgtgacg gttgtcagtg ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc
     5701 gccgagcagt cccattacca acacgatgcc ggtaatcgag caaacgacga tggtatccaa
     5761 aaacgtaccg gtcatagaaa ccaaggcctg acggacggga tggtcggttt tcgcggctgc
     5821 ggcggcaata ggcgcagaac ccatacccgc ctcattggag aacacgccgc gcgccacgcc
     5881 gtagcggatg accgtaccga tagcgccgcc cgccactgcc tgcgcgctga acgcatcgga
     5941 gaaaatcagc ttgacggcag gcatcagtgc atcggaatta atcgcgataa tggaaagacc
     6001 gcccaacaca taaaacaccg ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc
     6061 tttaatacca cctaaaacga caacggcagt cagaacggtc aacgtaatgc cggtataggt
     6121 aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc gagttggact gcaccgagct
     6181 gccgataccg aaggaagcga atgtgccgaa cagcgcaaat gcaacggcca tccatttcca
     6241 gtttttgccc aagccttttt cgatgtaata catcgggccg ccggacattt cgcctttgga
     6301 attgttgacg cggtatttca ccgccaacac gccttcgccg tatttggtgg ccatgccgaa
     6361 aatggcggtc atccacatcc aaaataccgc gcccgggccg ccggttacca ccgcagtcgc
     6421 cacgccggcg atgttacccg taccgatggt ggcggacagc gcggtcatca acgccgcaaa
     6481 atgggaaata tcgccttcgt ggccttcgcc gccttcatac ttctttgacg gcacaaacgc
     6541 ctgtttcagc gcatagccca acatagtgaa ctgtaagcct ttcagcataa cggtcagcac
     6601 aatgccggtg ccgaccagca gcatcaacat aacagatccc cagacccagc cgctgacggt
     6661 ttcgaaaaag gctttgagat tgtctaaaaa caattgcatg gctttctcct ttgtctgttt
     6721 tatttttaaa acaccacttt tgtagtgtcc tgtaatttca gcacagaata ttcaataaga
     6781 caatatgttc ttttgaaaaa tacttttggt tttttctccg aaagcaggtc ggttcaagct
     6841 gcggaaattg tttgcaattc tttaaaagca gcggcggagg tcacaatgaa atgtccgaat
     6901 ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac tgccatactt ctgaaatcta
     6961 caaaatgatg catcgatcaa acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg
     7021 ctttcggggt ttcagacggc atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat
     7081 tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg tttcggcgcg gcggagaatg
     7141 tctgccttgg cttcttcaaa cggaatgccg cgatggtaga gggttttgta cacgtctttg
     7201 acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc
     7261 gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt gtgtacgcct
     7321 gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga attggaaaac cagcgtgtag
     7381 ccgcccaaaa cgacgtagtc gccgacggta acgtgtccgg caagcgaggc gttgttggcg
     7441 aagatggtgt ggttgccgac cacgcagtcg tgcgcgaggt ggcagtacgc cataatccag
     7501 ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tgcctaaatt aaaggtggtg
     7561 aattcgcgga tggtgttgcc gttgccgata atcagcttgg tcggctcgtc gcggtatttt
     7621 ttgtcctgcg ggatttcgcc gaggctggca aattggaaaa tgcggttgtt ttcgccgatg
     7681 gtggtatgac cgctgatgac ggcgtgcggg ccgatttctg tattcgcgcc gatttggacg
     7741 ttggggccga taacggtgta cgcgccgact ttgacgctgg agtcgagttc ggctttgggg
     7801 tcgatgacgg cggtcgggtg gatgagggtc atgttttgaa tactccgctg cttgttaaga
     7861 caaataatag aaaatcacag tttgtcgatt tcatcgcaga ccgcaatcac ttgatcgcta
     7921 aaatggctaa atactgaagc agtatctaca tccatgtaat taagcagttc aaaaatagat
     7981 tggtgaatat ttctagcgta gttggtatct tttattgcac ctttaaaaca aatcggttcg
     8041 tgatgtgcca aacgattacg gaaattgtta atattggaaa gttctcgaaa aatccatttt
     8101 tggttatatt gcacacttgg tgtagatttg ggcttttttg gaaaaacttt catcaatact
     8161 ttccctgtag catcaaattg ggcatctttc cctcctgcaa acagataccg ccaaaaaccg
     8221 aatcctagtc ctgcaaccaa ttggttgtgg tcaaactttc ccccgctatt attttgtatt
     8281 ttagataatg caaccttgat taattcagca gattctttcg tgccatttct taaaaagcaa
     8341 ccttgatatt ttaatgcggg attcgtctgt ggctgtatgc tgttatataa ccagtttctg
     8401 tctttaaacg cctgctgaag gcaaatgtca attttgtttc ttaaaacaat ctcaaatatt
     8461 gaaacaacat gaaaaagttc tcttgataaa gataaattca ataaatacaa ttcagctgct
     8521 ttgtcttccc caacggcatt tttataccgt tccattctga aatcagaaaa aatactacag
     8581 cattccttat aattcacgct aacacctgat tctttatttt taaattgacg tttcaaacta
     8641 tctttgatac agttaggtaa aactaatccc caccgtgtcc ctgggtttga ataaacctaa
     8701 accggtgggt tttcttttta ttaggtcgtc tgaaactttc agacgacctt tttccgaacg
     8761 ctcaaaccac gcgtttggcg cacataatca cggcttcgac ggcaacttgc ccgtctactt
     8821 tggcaacggc gttgaatttg ccgatgccgc gtcggctggt cagcagctcg acttcaaaga
     8881 cgagttggtc gccggggatg acttggcgtt tgaaacgggc ttcgtctatg ccggcgaaga
     8941 agaagaattc gttttccttg cggccgcctt cgctgaggat ggcgagtgtg ccgcaggctt
     9001 gcgccatggc ttcgatgatg agtacgccgg gcatcacggg caggtcggga aagtggcctt
     9061 ggaactgggg ttcgtttatg gtgacgtttt tgatggcggt cagggttttc atggactcga
     9121 aggcggtgat gcggtcgagt tgcaaaaacg ggtagcggtg gggaatgagt ttttggatgt
     9181 ctttggcttc gatggggagt tgaacgtcca tgtctgtcgt attccttgaa taaagtcggt
     9241 ttggatttat tgcgtgtctt tgccgtctga aagctgctgt tccagcgttt tgaggcgttt
     9301 gttcatttcg cttaagcggt ggatgtaaac agcgttgcgc gcccattctt tatgggtgga
     9361 catcgggaag atgccggcga ggtgtttgcc gctttcggta atgctgtggg tgacggacgt
     9421 gccgccgccg atggtggttt tgtcggcgat ttcgatgtgt ccgaccgtac cgacgccgcc
     9481 gccgatgatg cagtagctgc ctatggttac gctgcctgag atgccggttt tggcggcgat
     9541 gacggtgtgc gaaccgattt tgcagttgtg tccgatttgg acttggttgt cgattttggt
     9601 gccgttgccg acggtggtgt cgctcatcgc gccgcggtcg atgttggtgt tcgagccgat
     9661 ttctacgtcg tcgcccagcg ttaccgcgcc ggtttgcggg attttaaacc atgaatcgcc
     9721 ggcaaaagcg agtccgaaac cgtccgcgcc gatgaccgcg ccgctgtgga tttcgacgcg
     9781 tctgcccagt gtgcagccgt aataaacgac ggcgttggga tgcaggacga cttcgtcgcc
     9841 cagtttgcaa tcgtgttgga cgacggcgtt tgccaagatg cggcagcctt cgccgagcac
     9901 ggtgtttgcg ccgatgtagg cgttcgcgcc gatttcgcag cttgcgggaa cggtcgcgtc
     9961 cggttcgacg acggcggtcg gatggatgcc gccgcgcgct ttgacgacgg gtgaaaacag
    10021 gcgggcgact ttggcgaaat agagataggg gtcgtcggcg acaatcaggt tgcgcccttc
    10081 aaatccgtct gccgctttgg cggaaacgat gaccgcgccc gcgctgctgt cgtggacttc
    10141 ggctttgtat ttcggattgg caaggaagct gatgtgttcc gcctgcgcgt ctgcgagcgg
    10201 gcgcacggcg gtaacggaaa tgtcctcgcc gcgccattcg ccgccgagcc gcgcggtgat
    10261 ttgggacagg gtgtaggtgg ccggaatcat ggttttcctg ttcggtatgc cgtctgaaag
    10321 ggtcagcggg cgttcatttc tttaatgacg ctgtcggtaa cgtcgtattg ggtgttgacg
    10381 taaatcacgt tctgcaaaat gacatcgtaa ccttcctgtt tggcgatttt gacgatgacg
    10441 cggttggcgt tttgctggag ggaggcaaac tcttcgttgc ggcggaggtt gtagtcttct
    10501 tcaaactgcg cctgtttttt gcggaacgct gcgaccagcc cgcgccattt ttcttcggct
    10561 tgcgcctttt ttgcgtttct gagtttgcct tcggcaagct gcctttccaa atccagacct
    10621 tcgcgttgca gtttttgcaa ttcgtcctga cgggcggaaa attcgctgtc cagcgttttt
    10681 tgaatcttgc gcgcctgctt ggattcgagg tagatgcgct cggtgttgat aaagccgatt
    10741 ttttggaagg tgtcggcgtg cgcgcctgcg gtgcagcaca aaccgatcag agccgcggca
    10801 aacgcgcggg tcaaacgggt catggtaaaa ctccttcgaa tgttgccgcg aaatgccgtc
    10861 tgaagggctt cagacggcat ttgcgggatt agaacgtcgt gccgagttgg aattggaagc
    10921 gttggatttc gtcttccggt tttttcttca gcgggtaggc gtagctgaat ttcatcgggc
    10981 ctaaaggcga gagccaggta accgcgccgc cggcggaata gcgcaattcg ttggtaaagg
    11041 tggatttatg ggtattgccg gcgccgtaaa tgttttgaac cctgccgccg gtcgcggaac
    11101 tgctgttgtc gtcgtaggtt ttgccgtccc acacgctgcc tgcgtcggca aacaggctca
    11161 ggcggacggt gcgcgcgtct ttcgcgccgg gcatcgggaa gagcagctcg gcggagacgt
    11221 tggctttttt gttgccgccg tagctgattt tttcgccgta ttcgtcatag actttcggac
    11281 cgagtgtgcc gctttcgtaa cctcgtaccg aacccaggcc gccgccgtag aagttttcaa
    11341 agaaggggat ttctttggtt ttgccgtagc cgttggcata gccgatttcg ccgccaagca
    11401 tcagtgtgaa ggttttgctc agggggaaga accaggtttg gttgtgggtg gcggagtagt
    11461 attgcagttt gctgccgggc agggcgattt cggcgttcac gcccgtcagg tagccgcgcg
    11521 tcggccataa cgcgctgtcg gttttgttgc gcccccagcc gacggtacct ttgtacagcc
    11581 agcctttgaa gctgccgtct gtgccgtcgg ttttgccgta tttcttgata aagtcggcat
    11641 agtgtttggg cgctttgttg taggtgttga cggtcaggtg ttctgccacc aaaccgaaat
    11701 tcacgcggtc gtattcggta acaggcacgc tcatgcggat gcctgcgcct gccgtggtgg
    11761 ttttatattg tttgatgctg gtcgatgctt tgcgcgggtc gaaggctttt ccgtaaacat
    11821 cgtagcccag gctgaccccg tctgccgtga agtacgggtc ggtaaacgac agcgagccgt
    11881 tgagcgtggt tttgcttcgt gaggcgcgca gggcggccga cttgcccgta ccgaacaggt
    11941 tgtcttggga aacgcctgcg gacatgacca ggccggtatc ctgtacccag cccgcgctca
    12001 agtcgagcga gccggtggaa cgttcggtca ggctcatgtt caaatcgact ttgtcgggtg
    12061 tgccggcaag cgggacggca tcaaactgta cgttgtcgaa gtagcccaaa agctcgacgc
    12121 gctctttgga gcgttgcagc ttggaggtgt cgtaaggcgc ggattccatt tggcgcaatt
    12181 cgcggcgcac gacttcgtcg cgggttttgt tgttgccggt gatgtggatt tcgttgacgt
    12241 agattttccg gcccggttcg atgtgcagga cgaaatcgac ggttttggtt tcggcgtttg
    12301 gcagcggctg tacgctgatt tcgctgtatg cgtagcctgc cgagcccatg cggttctgaa
    12361 tctcacccaa aacggcggtc atctgctggc gttcgtacca tttgccgggc ttcatggtca
    12421 gcagtttttc cagttcggct ttggggactt cgttggtgtc gccttcgatg gagactttgc
    12481 cccaacggaa acgttcgcct tcgtggacgg tgattttgat ggtctgcttg gttttgtctt
    12541 cgttggtttg gatgtcggta tcgaggatgc ggaaatcgaa gtagccgttg ttttggtaga
    12601 agtcggttac tttttccatg tcttgggcaa atttctgctc gttgaattgg ttgcttcgtg
    12661 tcagccatgt ccaaatgccg ccttcggtca gcgacatctg ccgcatcagt ttgcggtcgg
    12721 aatagacttg gttgccttca aattcgatgt cggtgatttt ggcggatttg ccctcgtcaa
    12781 tcgtgatgtc gatgtcgacg cggttgcggg cgagtttggt tactttgggc gtgatttgga
    12841 tattgagttt gccgcgcccg aggtattctt ctttcaggcc ggcgactgcc tgattgagtg
    12901 tcgcctgatt aaagtattgc gactgcgcca gcccgaacga ttcgagattt ttcttaatgg
    12961 cgtcgttttg cagcattttt gcgccggtga tgttgagcga gccgatggtg gggcgttcga
    13021 tgacggtcag caggagctgc ccgtccgcag tctcgacgcg tacgtcgtca aagaaaccgg
    13081 tggcgtacag gcttttgatg atggcactgc cgtgtgtgtc gttgtaggtg tcgccgactt
    13141 tgacgggcag gtagttgaat acggtactcg gctcggtacg ctgcaagcct tcgacgcgga
    13201 tgtcttggat ggtgaagtcg gcaagtgcca aaggcgatat gcccaagacc atcagtgcgg
    13261 aagcaatctg tttcagtttc attgtcagtt ccttgtggtg cggaatgcgg tttcagacgg
    13321 cattccgaaa cgtaaaatct aaccgagcag ccgggtaacg tcgttgaaga aggcgaccgc
    13381 catcatcagc atcatgaggg caagcccgaa gcgcaaaccg atgttttgga cgcgttcgcc
    13441 caaaggtttg ccgcgtatcc attcggcagt ataaaacacg aggtggccgc cgtccaaaac
    13501 ggggacgggc agtaggttca gcacgccgag gctgatgctg accagtgcca aaaattccaa
    13561 ataactttgc aagccgagtt cggcggactg tccggcaatg tcggcaatgg tcagcggccc
    13621 ggaaatatgg ctgacggagg cgttgccgct gattagtttg ccgaaaaatt tgagggttgt
    13681 ccacgagtgg gaaacggttt tttcccagcc catgccgaat gcgcggataa cagacggacg
    13741 gtagctgcgg cggatttgcg cgtcccacgc cctgtccggc tgcggacgga ggccgacgcg
    13801 cccgatcagg gtgtggtcgg actgttcgac agtatcgggg cggatgtcgg cggtatgggt
    13861 ttgtccggcg cgttcgtagt tcagggtgat ttttttgccg gggctttggc gggtcaggtt
    13921 tgcccattct tgccatgagg cgatgggttt gccgtcggcg gcagtcagcc tgtcgcccgg
    13981 tttcaggcct gctttttcgg cggggctgcc tttttccacg ccgccggcaa cggttgtgat
    14041 tttaaagggc atcagtccga tgtagccttg gttttttgcg attttaccgg cttccggcgt
    14101 gcctgcggca tcgatggtgc ggacggtttg cgcgcccgat gccgtctgaa cgccgacggc
    14161 gactttgccg gcttcgaggt tgaggacgat ttcggtttgc gcgctgcccc aatctgcaac
    14221 gggtgtgccg ttgacggatt gtattttgtc gccgctttgg aagccggcgc gggcggcaat
    14281 ggtgtcaggt tcgactgtgc cgacgtaggg gcgcagttcg gttacgccga aggaaaagct
    14341 cagtccgtac agcaaaaccg ccagtgcgag gttggtcagt gggccggcgg cgacgatggc
    14401 gatgcgcttg gcggggtgtt gtttgtcaaa agcgtagggt aaatcggctt ctgatacttc
    14461 gccttcgcgc gtgtcgacca ttttgacgta accgcccaac ggaatcgggg cgaggcacca
    14521 ttcggtgtcg ccgcgctttc gggtgaaaaa cggtttgccg aagccgacgg aaaagcgtac
    14581 gactttgaca ccgcacaatc tggcaacgat gtagtgtccg aactcgtgca ggctgaccaa
    14641 aatcaggatg gcgaagataa aagctagaag ggtgtgcaaa tggttttcct ttgataacgg
    14701 tgttcggacg gcatcagcgc agtgtgccga taaatgcccg cgcttgtgcg cgtgtccggg
    14761 catcttgcgc caagagcccc cctatgtcac ctatgccgtc tgaaaagtct tgtgaaagac
    14821 aatgggcgac ggttttggca atgtcggtaa acttaatctg tccgtccaaa aaggcggcga
    14881 cggcggcttc gttggcggcg ttcaatacgc agggcgcgac cccgcccgcg ttcatggctt
    14941 cataggcgag cttcaggcag gggaagcggt caaagtcggg cttttggaag gtcagcgcgg
    15001 acaatgcgtc gaaatcaagg tcgccgacac ccgaatcgat gcgctcgggc aagcccaaac
    15061 aataggcgat gggcgttcgc atatcgggat tgcccagttg tgccagcacg gagccgtcgc
    15121 ggtagcgcac catgctgtgt atcacggatt gcggatggat gacgacttcg agtttgtcgg
    15181 gcggacagtt gaacagccaa tgcgcttcaa tcagctccaa acctttgttc atcatggtgg
    15241 cggaatcgac ggagattttg cgccccatac gccaattggg gtgtttgacc gcttgggcgg
    15301 gcgtaatgcg gtcgaacgtg cctaaatcgg tcgttaaaaa cgggccgccg gaagcggtca
    15361 ggataatcga acggatgccg tgttcgttca gacggccggc gtaatcgcgc ggcaaaactt
    15421 ggaaaacggc gttgtgttcg ctgtcgacgg gcagcactgc cgcgccgttt gcacgggcgg
    15481 tttccataaa caacgcgccg gaaaccacca gcgtctcttt gttcgccaga taaatggttt
    15541 tgcctttttg cgccgctgcg agcgcggaag gcagccccac cgccccgacg atggcgcaca
    15601 tgacaccgct gacttcgtcg gcagaggcaa cgtcaaccaa tgcctgcgcg ccgtgtaaaa
    15661 cctgcgtcgc cgtgccgtcg cgtttcaaca gggcttcaag ccgggcggcg tattcggcat
    15721 cggcaacgac ggcatattcg ggttggaacg tttgacattg agccgccagt ttctcgacct
    15781 gcttatgccc tgccagcgcg aatacgcgga atttttcggg gtggcgggag acaacgtcca
    15841 gcgtgctttc gcctatgctg ccggtactgc ctaatatggt caggacttgt ggtgtcataa
    15901 tggggataac tttataccgg atgccgtctg aagcgttttc agacggcata gaatcaattt
    15961 aaaaccgaca tcatcgccgc atagacgctg ataacggcaa tcaggctgtc ggtacggtcg
    16021 aacacgccgc cgtgtccggg cagcagcttg ctgctgtctt tgatgcctgc cgcgcgcttg
    16081 agccagcttt ccaaaaggtc gccgcatacg ctgacaacgg tcaacaccaa accgattaac
    16141 acggtatcga accagcctgt atcgaatgcc agccagccgg cacttcgtac ggcggtcatg
    16201 tacacggcca cgcaaaccgc gccgccgatt gcgccttccc agcttttgcc ggggctgatt
    16261 gccggtgcga ttttgtgttt gccgaacgcc ttgccgctga aatacgcgca aatatcggca
    16321 acccacacca aacccatcac ggcgagcagc ggcagggcat catcgggatg cgggcgcagg
    16381 gatacgagcg cgaaccaaaa cggcatgacc agaagccagc cgacggcata aacctgccaa
    16441 ccgccgttga gcctccattt gaatctcaac cataaaggca taacggcgag ccaaaatgcc
    16501 aaaacaacat accaaaccaa attaggcagc atccagccgc ccgcataggc aaccacgccg
    16561 aaaaccaagg ttgcggcgag gtaatggttg gttttaattt tgcacaaacc gcccatacgg
    16621 gcatattccc acaaggcaat cagggcaatc agtccgcaaa atgcagccca caaccattgc
    16681 ggcgcgtaaa acagcatgcc cagcatcagc ggcagcagcc acatggcggt cattacccgt
    16741 tgtttcagca tattcagttc ctttgctgtt cgataggcag ttgctcggag gtgcgtccga
    16801 accgccgttc gcgtttttgg aacgaagcga cggcatcgtc caaagccttg ccgtcaaaat
    16861 cgggccacaa aatatcggtg aaatacagtt cggcataagc catctgccag agcaggaaat
    16921 tactgatgcg cgtttcgccg ccggtgcgga tgaacaaatc cggctcgggc gcgtctccca
    16981 gcatcaggta ttttgccagc gtgtctgccg taatctcgga tacgccttcg gcaatcagtt
    17041 tgtttgccgc ctgcaaaata tcccaacgtc cgccgtaatc ggcggcaatg ctcagggtca
    17101 ggccggtatt gtttgccgtc aaggcttccg cctcttcgat gccttgcaga atctgccgat
    17161 taaagcgttc gcggctgccc agtattttca ggcgcatatt gttttcgtgc aggcggcgta
    17221 cctgtttttg caaagcctgt aaaaacagcc ccatcaggaa cgaaacttcg tcttcggggc
    17281 ggcgccagtt ttcggtcgaa aaggcaaata cggtcagata ttgcacgccc aatttggcgc
    17341 aatgcctcac catattttcc aatgcgtcca aaccgcgttt gtgtcccatt atgcgcggga
    17401 ggaaacgttt tttcgcccaa cggccgttgc cgtccataat cacggcgata tgcttgggaa
    17461 tggcggtgtg ttccaaaacg gcctgcgtgc tgcttttcat gtctgccttt cgcggttcgg
    17521 cattcaaatg ccgtctgaac gccgaaccgt gcaggttaaa ttgccatcaa atcttcttct
    17581 ttggcagtca ggagtttgtc ggcttcggta atgtatttgt cggtcagttt ttgaaccgct
    17641 tcttcgccgc gacgtgcctc gtcttcggaa atttctttgt ctttgaggag ttttttgatg
    17701 tggtcgttgg catcgcggcg cacgttgcgg atggagacgc ggccttcttc cgcttcgccg
    17761 cgtacgactt taatcaggtc tttgcggcgt tcctcggtca gcatgggcat cggcacgcgg
    17821 atcaggtcgc cgacagctgc cgggttcagt cccaagtttg aatcgcggat ggctttctcg
    17881 actttggccg ccatattgcc ctcaaacggt ttcacgccga tggtgcgcgc atccagaagc
    17941 gttacgttgg caacttggct gacggggacc atgctgcccc agtattcgac ttccacttgg
    18001 tcgagcaggc cggtatgcgc gcggccggta cgcactttcg ccagattttc tttcagtact
    18061 tcgaccgaac gctgcatctt gccttcggct gttttttgaa tatcgttgat catattgttc
    18121 tttcggtaag ataaggtaag cgggcagccg tctgaaacgc gcttcaaacc tttcagacgg
    18181 catgaaaact gttaactgcg aatagtaccg ttattcgggc atgacgacaa ggcaggcgga
    18241 ttggggatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccga ccgtcagccg
    18301 tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc atccagccat
    18361 ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg catattggtg
    18421 ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc ttccagttcg
    18481 gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat aagggcgacg
    18541 gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt caagcagccg gatttcgctg
    18601 ccgttgagtt tgggagattg cagcttggcg gcagtggtca ggagcagctc ggtggtgttg
    18661 acaattttac ggtgggcgtg ctgcatggct tccatcatgg cggggctgat gcggctttcg
    18721 cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat ttggcgcatt
    18781 ttaaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct gatttcggca
    18841 atcattttgc tgcagtcggt caggttgtcg gcaagcatga aacgccacat cagtgtggat
    18901 ttcagcggca gcagtttggc ggcggcgatg gcgatggccg cgccgatgag gacgttcatc
    18961 gcgcgcatca ggccgctgtc gaaccattcg ctgccgttgt cgccgatgag catgcacatc
    19021 gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca gccggccagt
    19081 gcgcttgccg tgccgacggt gaggtagaag aggaggttgc cgtggaaata atgctggttc
    19141 agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg tgccgagcat acgttccacc
    19201 gccttggagt aaatcgcgcc ttgaaactgg agcatgccga ggacgacgaa gacggtcatc
    19261 cctatccact cgccgtgttg gaggtggagc agccgggcgg aggcggtggc gaacagaacg
    19321 gccccgccga gccggacggc gtggatgagg cggcggtagc ggtagcgttc gtaggagttg
    19381 agccagcggc tgacgaggcg gttgcgttgc gaggtgttca tatcggttgc cgtctgaagc
    19441 ggaaatgtga aaaagcacag gcttcccgag ggagggtctg tgcttggtat tggtgccgga
    19501 gaagggaatc gaacccccga ccttcgcgtt acgaatgcgc tgctctaccg actgagctac
    19561 accggcgttt ttcgttatga tatatatgaa cggttgtttg tgcaactttt ctggcgggcg
    19621 gcaaggcagt gcgcggtata gtg
//
LOCUS       DAWKOD010000022        19185 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000022 DAWKOD010000000
VERSION     DAWKOD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.022"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..123
                     /locus_tag="WJK73_000573"
     CDS             <1..123
                     /locus_tag="WJK73_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7557094.1"
                     /translation="ENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            141..452
                     /gene="rpsJ"
                     /locus_tag="WJK73_000574"
     CDS             141..452
                     /gene="rpsJ"
                     /locus_tag="WJK73_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ7557095.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(538..724)
                     /locus_tag="WJK73_000575"
                     /pseudo
     CDS             complement(538..724)
                     /locus_tag="WJK73_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            744..1796
                     /locus_tag="WJK73_000576"
     CDS             744..1796
                     /locus_tag="WJK73_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ7557096.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            1805..2488
                     /locus_tag="WJK73_000577"
     CDS             1805..2488
                     /locus_tag="WJK73_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ7557097.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            2707..3351
                     /gene="rplC"
                     /locus_tag="WJK73_000578"
     CDS             2707..3351
                     /gene="rplC"
                     /locus_tag="WJK73_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ7557098.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            3351..3971
                     /gene="rplD"
                     /locus_tag="WJK73_000579"
     CDS             3351..3971
                     /gene="rplD"
                     /locus_tag="WJK73_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ7557099.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            3968..4282
                     /gene="rplW"
                     /locus_tag="WJK73_000580"
     CDS             3968..4282
                     /gene="rplW"
                     /locus_tag="WJK73_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ7557100.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            4288..5121
                     /gene="rplB"
                     /locus_tag="WJK73_000581"
     CDS             4288..5121
                     /gene="rplB"
                     /locus_tag="WJK73_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ7557101.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            5128..5406
                     /gene="rpsS"
                     /locus_tag="WJK73_000582"
     CDS             5128..5406
                     /gene="rpsS"
                     /locus_tag="WJK73_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ7557102.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            5415..5744
                     /gene="rplV"
                     /locus_tag="WJK73_000583"
     CDS             5415..5744
                     /gene="rplV"
                     /locus_tag="WJK73_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ7557103.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            5754..6446
                     /gene="rpsC"
                     /locus_tag="WJK73_000584"
     CDS             5754..6446
                     /gene="rpsC"
                     /locus_tag="WJK73_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ7557104.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            6430..6846
                     /gene="rplP"
                     /locus_tag="WJK73_000585"
     CDS             6430..6846
                     /gene="rplP"
                     /locus_tag="WJK73_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ7557105.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            6846..7037
                     /gene="rpmC"
                     /locus_tag="WJK73_000586"
     CDS             6846..7037
                     /gene="rpmC"
                     /locus_tag="WJK73_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ7557106.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            7037..7300
                     /gene="rpsQ"
                     /locus_tag="WJK73_000587"
     CDS             7037..7300
                     /gene="rpsQ"
                     /locus_tag="WJK73_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ7557107.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            7522..7890
                     /gene="rplN"
                     /locus_tag="WJK73_000588"
     CDS             7522..7890
                     /gene="rplN"
                     /locus_tag="WJK73_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ7557108.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7902..8225
                     /gene="rplX"
                     /locus_tag="WJK73_000589"
     CDS             7902..8225
                     /gene="rplX"
                     /locus_tag="WJK73_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ7557109.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            8235..8774
                     /gene="rplE"
                     /locus_tag="WJK73_000590"
     CDS             8235..8774
                     /gene="rplE"
                     /locus_tag="WJK73_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ7557110.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            8777..9082
                     /gene="rpsN"
                     /locus_tag="WJK73_000591"
     CDS             8777..9082
                     /gene="rpsN"
                     /locus_tag="WJK73_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ7557111.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            9098..9490
                     /gene="rpsH"
                     /locus_tag="WJK73_000592"
     CDS             9098..9490
                     /gene="rpsH"
                     /locus_tag="WJK73_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ7557112.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            9504..10037
                     /gene="rplF"
                     /locus_tag="WJK73_000593"
     CDS             9504..10037
                     /gene="rplF"
                     /locus_tag="WJK73_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ7557113.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            10051..10404
                     /gene="rplR"
                     /locus_tag="WJK73_000594"
     CDS             10051..10404
                     /gene="rplR"
                     /locus_tag="WJK73_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ7557114.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            10423..10941
                     /gene="rpsE"
                     /locus_tag="WJK73_000595"
     CDS             10423..10941
                     /gene="rpsE"
                     /locus_tag="WJK73_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ7557115.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            10934..11119
                     /gene="rpmD"
                     /locus_tag="WJK73_000596"
     CDS             10934..11119
                     /gene="rpmD"
                     /locus_tag="WJK73_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ7557116.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            11121..11555
                     /gene="rplO"
                     /locus_tag="WJK73_000597"
     CDS             11121..11555
                     /gene="rplO"
                     /locus_tag="WJK73_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ7557117.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            11567..12877
                     /gene="secY"
                     /locus_tag="WJK73_000598"
     CDS             11567..12877
                     /gene="secY"
                     /locus_tag="WJK73_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ7557118.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            12882..13100
                     /gene="infA"
                     /locus_tag="WJK73_000599"
     CDS             12882..13100
                     /gene="infA"
                     /locus_tag="WJK73_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ7557119.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            13121..13234
                     /gene="rpmJ"
                     /locus_tag="WJK73_000600"
     CDS             13121..13234
                     /gene="rpmJ"
                     /locus_tag="WJK73_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ7557120.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            13300..13662
                     /gene="rpsM"
                     /locus_tag="WJK73_000601"
     CDS             13300..13662
                     /gene="rpsM"
                     /locus_tag="WJK73_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ7557121.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            13682..14077
                     /gene="rpsK"
                     /locus_tag="WJK73_000602"
     CDS             13682..14077
                     /gene="rpsK"
                     /locus_tag="WJK73_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ7557122.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            14097..14717
                     /gene="rpsD"
                     /locus_tag="WJK73_000603"
     CDS             14097..14717
                     /gene="rpsD"
                     /locus_tag="WJK73_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ7557123.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            14743..15729
                     /gene="rpoA"
                     /locus_tag="WJK73_000604"
     CDS             14743..15729
                     /gene="rpoA"
                     /locus_tag="WJK73_000604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ7557124.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            15753..16121
                     /gene="rplQ"
                     /locus_tag="WJK73_000605"
     CDS             15753..16121
                     /gene="rplQ"
                     /locus_tag="WJK73_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ7557125.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            16268..16981
                     /gene="minC"
                     /locus_tag="WJK73_000606"
     CDS             16268..16981
                     /gene="minC"
                     /locus_tag="WJK73_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ7557126.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            17009..17824
                     /gene="minD"
                     /locus_tag="WJK73_000607"
     CDS             17009..17824
                     /gene="minD"
                     /locus_tag="WJK73_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ7557127.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            17828..18091
                     /gene="minE"
                     /locus_tag="WJK73_000608"
     CDS             17828..18091
                     /gene="minE"
                     /locus_tag="WJK73_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ7557128.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            18095..19015
                     /gene="oxyR"
                     /locus_tag="WJK73_000609"
     CDS             18095..19015
                     /gene="oxyR"
                     /locus_tag="WJK73_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ7557129.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
BASE COUNT         5283 a         3635 c         4827 g         5440 t
ORIGIN      
        1 gagaacgtaa ccatcaccgt agaactgatt gcgcctatcg ctatggaaga aggtttgcgc
       61 tttgcgattc gcgaaggcgg ccgtaccgtg ggtgccggcg tggtttcttc tgttatcgct
      121 taattgaagg atattgataa atggcaaacc aaaaaatccg tatccgcctg aaagcttatg
      181 attacgccct gattgaccgt tctgcacaag aaatcgttga aactgcaaaa cgtaccggtg
      241 cagttgtaaa aggcccgatt cctttgccga ccaaaatcga acgtttcaac attctgcgtt
      301 ctccgcacgt gaacaaaact tcccgtgaac aattggaaat ccgcacccac ttgcgcctga
      361 tggacatcgt ggattggacc gacaaaacta ccgatgcgct gatgaagctg gatttgccgg
      421 ccggtgttga tgtagaaatc aaagtccaat aattagggct gttgaaaatc cccaagcaat
      481 taatgcttgg ggatttttta tgttatgccg tctgaaaata tgcggcggca aacagattta
      541 aggcaaaaca acaaaatcaa aaaacagtgc ggtcggcgaa atcggcttat tttgtgcctc
      601 aagaattttc agacggcatt ttttagtgaa caaaacagga tattttttat ccatagcctt
      661 ccgaaggctt gccccccccc cgaagaaagg tagaatcggg cttggtttgg gcagaagcat
      721 ccatctgatt ctaaggatgg atgatgtttt attctcaagt aaataaagga aagatgatga
      781 gcttacttaa tgtctttaaa gatattttaa acaacaaaga attgaattcg gaagaacggc
      841 atgatttgga gcgggcggtt aagatcttgc aggatacgca tgtcaatatt ttgattaccg
      901 gagccaccgg ttgcggaaaa tcatccacca tcaatgcctt gttcagcata gatcaggcaa
      961 cgggaaagag tgatggacta gtaaaggaag tcgcaaaagt aggcgtgggt gtagatcctg
     1021 aaactatgga tattgccaaa tatgaattgg agaatatgac gatttgggat agtccgggct
     1081 tgggcgacgg agagaaggca gaccaaaagc accggcgcaa tatcatcaat aagctgctgg
     1141 agaaagacag taacggcaat gccgtcatcg acttggtttt ggtgctgctt gacggcggct
     1201 ccagagactt ggggacatca tacgaactga ttaacaatgt aatcatcaat acattgaaga
     1261 agagtgggga aggccgtaca gaccggttgc tggttgcgat taatcagtgt gatatggcga
     1321 tgaaggggcg gaattgggat gcggaagccg gccgtccgaa cgccgccttg gaaaactttt
     1381 tggaggagaa agtgtgttcg acacgcaagc gcattcagga agctaccggc gttacggtca
     1441 atccgattta tttttcagca ggctttaaga gtgaggatga ggagcagcgt ccctataaca
     1501 taacccgcct gttgcgttac atcattacgg ctatgccgtc tgaaaaacgg gtgattgtgg
     1561 cagagaagat taatgtggaa gaaatcgaac gagataccag ccgccgcaaa caggaggagc
     1621 ggcaagaaat cgacagtagt atcggcagtg cgctggagaa aatattgggg gctgcgggag
     1681 gcgtggttaa agatgttgtt tcaacgatag caagcggtgt cgggacgctg atcggcggtg
     1741 ctgcaaaagc gattggaggg gcgattagca cggttgcttc tttttttggc tggtaggagc
     1801 gcatatgcat cgttaccgta tttctgaaat cgggcaaaaa gtcacagcag ccggtttccg
     1861 tcatttggat gtcttgcttg tcggtgcgac aggtgtcggc aagtcctcaa ccttaaatgc
     1921 cttgtttggc gaacaaaaag ccaaggtagg cacaggcgtg gatcccgaaa cgcaattggt
     1981 cgaaagctat atgctgaacg atgtgttgcg gttttgggac agcgcaggtt tgggcgatgg
     2041 gaaagaagcc gaccgcgccc atcggcaaaa actgattgat gtcctgtcta aaacctatac
     2101 tcattcggat gggcagtggg gctggataga tttggtgttc gttatccttg acggcagctc
     2161 ccgcgatttg ggtacggcct atgatttgtt gagggatgtt atccttaaaa tgattgatcc
     2221 ggatagggtt attgttgcaa tcaatcaggc ggatatggcg atgaaaggac gttattggga
     2281 taaggtgctg catcagccgc agccgaattt gcaacagttt ctagacgaga aggccgaatc
     2341 ggtacaaaaa aggattctgg aagcaaccgg tttgcagatt tccaaacccg tttactattc
     2401 ggcatacgaa aactaccatc tcaaagaaat aatggatgca gtcatcaacc atatcccggt
     2461 ttgtcggaga aagatgcata cgccgtgatg gtcggatacc gactccttta aaagcatccg
     2521 accctatgcc gtctgaagat tcaaaacgct tcagacggca tattgaagat atttttgata
     2581 tttttgttga tatttctttg acttgtcaga tataatgtct gacttggtac attcgtacca
     2641 agtttaactt tgtctgaaag acaggccaat cgtagcctgt ccctttactt taaaaggaaa
     2701 ataatcatga ctttaggtct ggttggacgc aaagttggta tgacccgcgt gttcgacgaa
     2761 cagggtgttt ctgttccggt aaccgttttg gatatgtctg ccaaccgcgt tacacaagta
     2821 aaatccaaag atactgacgg ctatactgcc gttcaagtta cctttggtca gaaaaaagcc
     2881 aatcgtgtca acaaagccga agccgggcac tttgcaaaag caggtgttga agccggtcgc
     2941 ggtttgattg agtttgcttt gactgaagaa aaactggctg aattgaaagc tggtgacgaa
     3001 atcaccgttt ctatgtttga agtcggtcaa ctggtcgatg taaccggtac ctctaaaggt
     3061 aaaggtttct ccggcacgat caaacgtcat aacttcggtg cccaacgtac ttcccacggt
     3121 aactcccgtt ctcaccgtgt tccaggctct atcggtatgg cgcaagaccc gggtcgcgtg
     3181 ttccccggta aacgcatggc cggccaatac ggcaacacca aagcaactgt tcaaaaattg
     3241 gaagttgtcc gtgttgatgc agaacgccaa ctgctgttgg ttaagggtgc tgttccgggt
     3301 gcggtcaaca gcgatgttgt agttcgtccc agcgtgaaag taggtgcgta atggaattga
     3361 aagtaattga cgctaaagga caagtttcag gcagcctgtc tgtttctgat gctttgttcg
     3421 cccgcgaata caatgaagcg ttggttcatc agctggtaaa tgcctacttg gcaaacgccc
     3481 gctctggtaa ccgtgctcaa aaaacccgtg ccgaagtaaa acactcaacc aaaaaaccat
     3541 ggcgtcaaaa aggtaccggc cgcgcccgtt ccggtatgac ttcatctccg ctgtggcgta
     3601 aaggtggtcg cgcgttcccg aacaaacccg acgaaaactt cactcaaaaa gtaaaccgca
     3661 aaatgtaccg tgccggtatg gcgactattc tgtcccaatt gactcgtgac gagcgtttgt
     3721 ttgcgattga ggcgttgact gctgaaactc ctaaaaccaa agtttttgct gaacaagtga
     3781 aaaatctggg tctggagcaa gtgttgtttg taaccaaaca gctcgacgag aatgtttact
     3841 tggcttcacg caacttgcca aacgtgttgg ttttggaagc tcaacaagtt gatccttaca
     3901 gcttgctgcg ttacaaaaaa gtaatcatca ctaaagatgc agttgcacaa ttagaggagc
     3961 aatgggtatg aatcaacaac gtttgactca agtgattttg gcacctatcg tttctgaaaa
     4021 aagcaacgta ttggctgaaa aacgcaacca aatgacgttt aaagtcttgg caaatgcaac
     4081 caaacctgaa atcaaagctg ctgttgagct gctgtttggt gttcaagttg cttctgtaac
     4141 taccgttaca actaaaggca aaactaagcg ttttggtcgt actttgggcc gccgcagcga
     4201 tgttaaaaaa gcttatgtaa gcttggctgc cggtcaagag ttggatttgg aagccgctgc
     4261 tgcagctgca gataaggaat aaacaaaatg gcaatcgtta aaatgaagcc gacctctgca
     4321 ggccgtcgcg gcatggttcg cgtggtaaca gaaggtttgc acaaaggtgc accttatgcg
     4381 cccttgcttg aaaagaaaaa ttctactgcc ggccgtaaca ataatggtca tatcaccacc
     4441 cgtcacaaag gcggcggtca taaacaccat taccgtgttg tagactttaa acgtaacaaa
     4501 gacggcattc ctgccaaagt agagcgtatc gaatacgatc ctaaccgtac tgccttcatc
     4561 gcactgttgt gctatgcaga cggcgagcgt cgttacatca ttgctcctcg cggtattcaa
     4621 gccggtgctg tattggtttc cggtgctgaa gctgccatca aagtaggcaa caccctgccg
     4681 attcgcaaca ttcccgttgg tacgactatc cactgtatcg aaatgaaacc cggtaaaggt
     4741 gctcaaatcg cacgttctgc cggtgcttct gcggtattgc tggctaaaga aggcgcgtac
     4801 gctcaagtcc gtctgcgctc tggcgaagtc cgtaaaatca acgtaaattg ccgtgcgacc
     4861 attggtgaag ttggtaacga agagcaaagc ctgaaaaaaa tcggtaaagc cggtgccaac
     4921 cgttggcgcg gtattcgtcc gaccgtacgc ggtgttgtca tgaatcctgt cgatcacccg
     4981 catggtggtg gtgaaggccg tacgggcgag gcccgcgaac cggtcagccc atggggtact
     5041 cctgctaaag gctaccgcac tcgtaataac aaacgcacgg ataacatgat tgttcgtcgc
     5101 cgttactcaa ataaaggtta atttagtatg gctcgttcat tgaaaaaagg cccatatgta
     5161 gacctgcatt tgctgaaaaa agtagatgct gctcgcgcaa gcaacgacaa acgcccgatt
     5221 aaaacctggt ctcgtcgttc taccattctg cctgatttta tcggtctgac cattgctgtg
     5281 cacaacggcc gcacccatgt gcctgtgttt atcagcgaca atatggttgg tcataaatta
     5341 ggcgaattct cattgacccg tacctttaaa ggccacttgg ccgataaaaa ggctaaaaag
     5401 aaataaggtg aatcatgaga gtaaatgcac aacataaaaa tgcccgtatc tctgctcaaa
     5461 aggctcgttt ggtagctgat ttgattcgtg gtaaagacgt tgcccaagct ttgaatattt
     5521 tggctttcag tcctaaaaaa ggtgccgagc tgattaaaaa agtattggag tcagctattg
     5581 ctaatgccga gcacaataac ggtgcggaca ttgatgaact gaaagtggta actatctttg
     5641 ttgacaaagg cccaagcttg aaacgttttc aagctcgcgc caaaggtcgc ggtaaccgca
     5701 tcgaaaaaca aacttgtcat atcaatgtga cagtgggtaa ctaaggaaaa gctatgggac
     5761 aaaagattaa ccctacaggc tttcgcctgg cggtaactaa agactgggct tcaaaatggt
     5821 ttgctaaaag caccgacttt tctactgttt tgaagcagga tatcgatgtt cgcaattatt
     5881 tgcgtcaaaa attggccaat gcttcggttg gtcgagtggt tattgaacgc cctgcaaaat
     5941 ctgcacgcat taccattcac tccgctcgtc cgggtgtggt tataggtaaa aaaggtgagg
     6001 atatcgaggt tttgaaacgt gacttgcaag tcttgatggg tgtacctgtt catgtaaata
     6061 ttgaagagat tcgccgtcct gagttggatg ctcaaattat tgctgacggt attgcccagc
     6121 agttggaaaa gcgcgttcaa ttccgtcgtg ctatgaaacg agcaatgcaa aatgcaatgc
     6181 gttctggtgc taaaggcatt aagattatga cttcaggccg tctgaatggt gcggatattg
     6241 cccgtagcga atggtatcgt gaaggtcgcg tgccactgca tactttacgt gcaaatgtag
     6301 attatgcaac cagcgaagcg cacaccacat atggtgtatt gggtctgaaa gtttgggttt
     6361 atacggaagg caatattaaa tcttccaaac ctgaacatga gagtaaacaa agaaaggcag
     6421 gtagacgtaa tgctgcagcc aactagactg aaataccgta agcaacaaaa gggtcgcaat
     6481 actggcatcg ctactcgcgg taataaggta agtttcggtg agttcggctt gaaagccgta
     6541 ggtcgtggtc gtttgactgc ccgtcaaatc gaagctgctc gtcgtgcaat gacccgtcat
     6601 atcaaacgtg gtggtcgtat ttggattcgt gtattccctg ataaaccgat tactgaaaag
     6661 cctattcaag ttcgtatggg tggcggtaaa ggtaacgtgg aatattacat tgccgaaatt
     6721 aaaccaggta aagtgttgta tgaaatggat ggcgttccag aggaactggc tcgtgaagca
     6781 ttcgagttgg ctgctgccaa attgcctatt cctacaacct ttgtagtaag acaggtgggt
     6841 caataatgaa agcaaatgaa ttgaaagaca aatccgttga gcagttgaat gcagatttgt
     6901 tggacttgtt gaaagctcag tttggcttac gtatgcaaaa cgctaccggt caattaggca
     6961 aaccaagtga attgaaacgt gtacgtcgcg atattgctcg tattaaaacc gttttaactg
     7021 aaaaaggtgc taagtaatga gcgaaactaa aaatgttcgt actttgcaag gcaaagtagt
     7081 aagcgacaaa atggataaaa ccgtaacagt attggttgag cgtaaagtaa aacatccgct
     7141 gtatggtaag attattcggt tatctactaa aatccatgcc catgatgaaa ataatcaata
     7201 tggaattggt gatgtggttg ttatatcgga atcccgtcca ttgtcaaaaa ctaaatcttg
     7261 ggttgtcagt gagctggttg agaaagcacg ttctatttaa gaattaaagc aacgtgcttg
     7321 gaatgggaaa cgaagtattg cagcaaattt aatttgcgtg taaacttcgt ttcctgtctt
     7381 tcagtttctt ctggaagttt cttccctttc ggggtccaag actggtttac ttgaaccgca
     7441 aggtttcatt taataagcag cggctttgct gtaagttatc tgaaagtggt aaattaagtt
     7501 ggttaattta aaggtaataa catgattcaa atgcagacca tcttagatgt ggctgataac
     7561 tctggtgcgc gtcgcgtaat gtgtatcaag gtattgggcg gatctaagcg tcgctacgct
     7621 tctgttggcg atattattaa agtggcagtt aaagatgcgg ctccgcgtgg ccgtgtcaaa
     7681 aaaggcgatg tatataatgc ggtagttgtt cgtactgcta agggtgtacg tcgtcctgat
     7741 ggtgcgttaa ttaaattcga taacaatgcc gccgtgttac tgaataataa acttgaacct
     7801 ttgggtactc gtatctttgg tccggtaacc cgtgaattgc gtactgagcg atttatgaaa
     7861 atcgtttcat tggcacctga agtattataa ggaatggcac gatgaataaa atcattaaag
     7921 gcgatagggt tgtagtaatt gctggtaagg ataaaggtaa gcagggtcaa gtagttcgag
     7981 tgttgggtga taaagttgtt gttgagggcg ttaatgttgt aaaacgccat caaaaaccta
     8041 atccaatgcg tggcattgag ggcggtatta ttactaaaga aatgcctttg gatatttcta
     8101 atatcgcaat cctgaatccg gaaactaata aagcggaccg tgttggtatt aagctgattg
     8161 aaaatgaagg caaagttaaa cgcgttcgtt tcttcaaatc aaatggctct atcattgggg
     8221 cataaggaga taacatggct cggttgagag agttttataa agagacagtt gttcctgaat
     8281 tggttaaaca atttggttac aaatcagtaa tggaagtccc gcgtattgaa aaaatcacct
     8341 tgaatatggg tgtgggtgag gctattgctg ataaaaaagt tatggaacat gctgtttccg
     8401 atttagagaa aattgccggt caaaaaccgg ttgttactgt tgcccgtaaa tctatcgcag
     8461 gttttaaaat ccgtgataac tatccggttg gttgcaaagt aacattgcgt cgtgatcaaa
     8521 tgtttgaatt cttggatcgt ttgattacta ttgcattacc tcgcgtacgt gacttccgtg
     8581 gtgtgagcgg taaatcattt gatggccgtg gcaattacaa tatgggtgtt cgtgagcaaa
     8641 ttatttttcc ggaaattgaa tacgataaaa ttgatgcttt gcgtggtttg aatattacta
     8701 ttactactac agcaaaaacc gatgaggaag cgaaagcttt attgtcattg tttaaatttc
     8761 cgttcaaagg ataatcatgg ctaagaaagc acttattaat cgtgatctga aacgtcaagc
     8821 tttggctaaa aaatatgcgg ctaaacgcgc ggcaattaaa gcggtaatca atgattcgaa
     8881 tgcaactgag gaagagcgtt ttgaggctcg tttgaggttt caatccattc ctcgtaatgc
     8941 ggcacctgtg cgtcaacgtc gtcgttgtgc tttgacaggt cgccctcgtg gtactttccg
     9001 taaatttggt ttgggtcgta ttaaaatccg tgaaatcgcc atgcgtggcg aaattccggg
     9061 tgttgttaaa gccagctggt aataggagta attaagaatg agtatgcatg atcctatttc
     9121 cgatatgttg actcgtatcc gcaatgcgca acgtgctaat aaagcagcgg ttgcaatgcc
     9181 ttcttcaaaa ttaaagtgtg ctattgcaaa ggtattgaaa gaagaaggat atattgagga
     9241 cttcgcagtt tcatctgacg taaagtctat attggaaatt caattaaaat actatgcagg
     9301 tcgtcctgta attgaacaaa tcaagcgtgt atctcgcccc ggtttgcgta tttataaagc
     9361 gtctagtgag attccaagtg ttatgaatgg cttgggtatt gctattgtta gtacttctaa
     9421 aggtgtaatg actgatcgta aagcacgttc tcaaggtgtt ggtggtgagt tgttatgcat
     9481 tgtagcctag tggaggaaaa gaaatgtcac gtgtcgcaaa aaacccagtg actgttcccg
     9541 ctggtgtaga agtaaaattt ggaacagagg cattagttat taagggtaag aacggtgaat
     9601 tgtcttttcc tttgcattct gatgtagcca ttgaatttaa tgatggcaaa ttggcttttg
     9661 ttgcgaataa cagcagtaaa caagcaaatg caatgtctgg tactgctcgc gcattagtca
     9721 gcaatatggt taaaggtgtt tcagaaggtt ttgagaaaag attgcaattg ataggtgtgg
     9781 gttatcgtgc tcaagcacaa ggtaaaatct tgaatctgtc tttgggtttt tctcatccga
     9841 tcgtatatga aatgcctgaa ggtgtctccg ttcaaactcc tagccaaaca gagattgttt
     9901 taaccggctc ggataaacaa gttgttggtc aagttgctgc tgagattcgt gcgttccgtg
     9961 ctcctgagcc ttataaaggt aaaggtgttc gctatgtagg cgaagtagtg gtaatgaaag
    10021 aagccaagaa aaaataattg aggttcacta atggataaac atacaacccg actccgtcgt
    10081 gcacgcaaaa cccgtgctcg tattgcggac ttgaaaatgg taagattatg tgtgttccga
    10141 agcaataatc atatttatgc tcaagtaatt agtgctgaag gtgataaagt attggctcaa
    10201 gcctctacat tggaagctga ggtgcgcggt agtctgaaat ctggaagcaa tgttgaagca
    10261 gctgcaatag ttggtaaacg tatcgctgag aaggctaaag cagcaggtgt agaaaaggtt
    10321 gcttttgatc gttcaggttt ccaatatcac ggtcgtgtga aggctttggc tgaagctgct
    10381 cgtgaaaatg gtttaagctt ctaaatattt ggagactttc agatggcaaa acatgaaatt
    10441 gaagaacgcg gtgacggtct gattgaaaag atggtcgcag ttaaccgtgt aaccaaagta
    10501 gttaaaggtg gtcgcattat ggctttctct gcgctaactg ttgttggtga tggagatggt
    10561 cgtattggta tgggtaaagg taagtcaaaa gaagtgcctg ttgcagtcca aaaggcgatg
    10621 gatcaagcac gacgctctat gattaaggta ccattaaaaa atggtacgat tcatcatgag
    10681 gttattggtc gacatggtgc tactaaagta tttatgcagc ctgctaaaga gggtagtggc
    10741 gtaaaagccg gtggacctat gcgtttggtt tttgatgcta tgggcattca taatatctcc
    10801 gccaaagtgc acggatctac taacccatat aatatcgtac gtgcaacatt agatggtttg
    10861 tctaagttgc atactcctgc tgatatcgca gccaaacgtg gcttgacagt ggaagacatt
    10921 ttgggagtta accatggctg aacaaaaaaa gattagggtt acattggtta aaagcctgat
    10981 tggtacaatt gaatctcatc gtgcatgtgc acgcggttta ggtttgcgtc gtcgcgagca
    11041 tacggtagag gttttagata cccctgaaaa ccgtggtatg attaataaaa tcagctactt
    11101 gttgaaagtg gagtcttgat atgtttttga atacaattca acctgctgtt ggtgctacgc
    11161 atgctggtcg tcgtgttgga cgcggtattg gtagtggtct tggcaaaacg ggtggtcgtg
    11221 gtcataaagg tcaaaagagc cggtctggtg ggtttcataa ggtgggtttc gaaggtggtc
    11281 aaatgccctt gcaacgacgc ctccctaaaa gaggttttaa atctttaaca gcatcagcta
    11341 atgcacagct tcgtttaagt gaactggaat caattgctgt taatgagatt gatattttgg
    11401 tcttaaagca agcgggtctg attgcatcta cagtctctaa tgttaaagtt attgcttttg
    11461 gtgaaatttc taaggcagtt gctttgaagg gtattaaagt taccaaaggt gcgagagctg
    11521 ctatcgaggc tgttggtggt aagattgaaa tgtaaggttt aatattgtgg ctaatcaaca
    11581 aacgtcatca ggttcatcca aatttggaga tcttaagaaa cgtcttttgt ttctatttgg
    11641 agcattgatt gtttttcgaa ttggtgccca tatacccgta cctggagttg atgctgttgc
    11701 tttagctaaa ttatacgaaa gcgctggaaa cggcatcctg ggaatattga atatgttttc
    11761 cggtgggtcg ttagagcgct ttagtatatt tgcaatagga attatgccat atatttcagc
    11821 ttctattatt gtacagctcg cttctgaaat tttgccatca ttgaaggctt taaaaaaaga
    11881 aggggaggct ggtagaaagg taattacgaa atatactagg tatggtactg ttttgttagc
    11941 aattcttcaa agtctaggtg ttgcatcttt cgtatttcag caaggaattg ttgtaacaag
    12001 ttcatttgag tttcatgttt ccacggtagt ttctttggta acgggaacta tgtttcttat
    12061 gtggcttggg gagcaaatta ctgaaagggg tatcgggaac ggtatttctt taatcattac
    12121 ggcaggtatt gcttcaggta ttccttcggg tattgcaaag ctggttacac tgacgaacca
    12181 aggttctatg agcatgctta cggcgttgtt tattgtattt ggtgccttat tattaattta
    12241 tttggttgta tactttgaaa gtgcacagcg gaagattcct attcattatg caaaacgcca
    12301 gtttaatggt agggcgggta gtcaaaatac gcatatgcct ttcaagttga atatggctgg
    12361 tgttattccc ccaatttttg cttccagtat tattctattt ccatctactc ttttaggttg
    12421 gtttggttcg gctgatacaa atagtgtttt gcacaaaata gctggattgt tacaacacgg
    12481 tcaattgctg tatatggctt tatttgcagc gacagttatt ttcttttgtt atttttatac
    12541 ggctttggtt tttagcccta aagaaatggc agagaattta aaaaagagtg gtgcttttgt
    12601 tcctgggatt agacctggtg agcagacctc taggtattta gaaaaagttg tattacgttt
    12661 gacattgttt ggagctcttt atattacaac tatttgttta attccagagt tcttaactac
    12721 ggttttaaat gtaccttttt atttgggtgg cacgtctttg ttgattctag ttgttgtaac
    12781 gatggatttt agtacacaaa taaattcgta taggcttact caacagtatg ataagttaat
    12841 gactcgttca gaaatgaaat cattttctcg gaaatagaat tatggcgaaa gaagatacta
    12901 tccaaatgca aggtgaaatt cttgaaactt tacctaatgc aacatttaaa gtaaaacttg
    12961 agaatgacca tattgtattg ggtcatattt ctgggaagat gcggatgcat tacattcgta
    13021 tttctccggg agataaggtc acagtagagc tgacacctta tgatctaact agggctcgaa
    13081 tcgttttcag agcaagataa accaataaaa ggaaaataaa atgcgtgtac aaccatctgt
    13141 taagaaaatt tgccgaaatt gcaagattat tcgtcgaaat cgtgtagttc gtgtaatttg
    13201 tactgatctc cgtcacaaac agcgtcaagg ttaatggaat atttctttta atgtgattct
    13261 gtgatatagt gacacacttt gccctaaaaa ggaaaaaata tggctcgtat tgcaggggta
    13321 aatatcccta ataacgcaca catcgtaatt ggtcttcagg ctatttacgg tattggtgct
    13381 actcgtgcta aattgatttg tgaggctgca aatattgcgc ctgatactaa agcaaaagat
    13441 ttggacgaga ctcaattaga tgctttgcgt gaccaagttg ccaagtatga agtagaaggt
    13501 gatttgcgtc gtgaagtaac tatgagtatc aagcgattga tggacatggg ctgctatcgt
    13561 ggcttccgtc atcgtcgcgg cttaccatgc cgcggtcaac gcactcgtac aaatgcgcgt
    13621 acccgcaaag gtccgcgtaa agcgattgct ggtaagaaat aaattttaag gaattttatt
    13681 aatggctaaa gcaaacacag cttcacgtgt acgtaaaaaa gtacgtaaaa ccgtgagtga
    13741 gggtattgtg cacgttcatg catctttcaa caataccatc attacaatca ctgaccgtca
    13801 aggcaatgcg ttgtcttggg ctacctctgg cggcgctggt tttaaaggtt ctcgtaaaag
    13861 tacaccattt gcagcacaag ttgcagcaga agcagctggt aaagttgccc aagagtatgg
    13921 cgttaaaaat ttagaggttc gtattaaagg tccaggtcca ggtcgcgaat cctctgtacg
    13981 tgctttgaat gctcttggtt tcaagattac cagcattact gacgttaccc cgttgcctca
    14041 taacggttgc cgtccgccta aaaaacgtcg tatttaatat tggagtgatt tgaaacatgg
    14101 cacgttatat tggccctaaa tgtaagttgg cacgtcgcga aggtacggat ttgtttttga
    14161 agagtgcgcg ccgctctttg gattctaaat gtaaaattga ttccgctcct ggtcagcatg
    14221 gtgcaaaaaa accgcgtttg tcagactatg gtttgcagtt gcgtgaaaaa caaaaaatcc
    14281 gccgtattta tggcgtatta gaacgtcagt tccgtcgtta tttcgcagaa gctgatcgtc
    14341 gtaaaggttc tactggcgag ttgctgttgc agttgctgga atctcgtttg gataatgtcg
    14401 tttatcgtat gggtttcggt tctacccgag ctgaagcaag acagcttgtt tctcataagg
    14461 cgatagttgt gaatggacaa gttgtcaata ttccttcttt ccaagtgaaa gctggtgatg
    14521 ttgtctcagt tcgtgaaaaa gccaaaaaac aggtacgtat tcaagaagca ttgggtttgg
    14581 caactcaaat cggcttgccg ggttgggttt ctgtagatgc ggataaactt gagggtgtgt
    14641 tcaaaaacat gccggatcgc tcggaattga ccggtgatat taatgaacag ctggtggtag
    14701 agttctactc taaataatgc tagctcagtg agggacagtt aaatgcagaa tagcacaacc
    14761 gaatttttga aacctcgtca aattgatgta aatacttttt ctgcaactcg tgcaaaagta
    14821 tctatgcagc catttgaacg tggtttcggt cataccttag gtaatgcttt gcgccgtatc
    14881 ttactgtcat ccatgaatgg ttttgctcct actgaagtag ctattgccgg tgtattacac
    14941 gaatattcta ctgttgatgg tattcaggaa gatgttgttg acattttgct gaatattaaa
    15001 ggtattgtgt ttaaactcca tggtcgtagc caagttcaac ttgtgttgaa gaaatcaggt
    15061 tcaggtgtcg tatctgccgg tgatattgag ttgccgcatg atgtagaaat tctgaatcct
    15121 ggtcatgtca tttgtcattt ggctgataac ggtcaaattg agatggaaat taaagtagag
    15181 caaggtcgtg gttatcaatc tgtttcaggt cgtcaggtag ttcgtgatga gaaccgtcag
    15241 attggtgcaa tccagttgga tgcgagcttt tcgcccatca gccgtgttag ctttgaggtt
    15301 gaacctgcac gtgtagagca gcggacggat cttgataagt tggttttgga tatcgaaacc
    15361 gacggttcta ttgatcctga ggaagctgta cgcagtgcgg cacgtatttt gattgatcag
    15421 atgtctattt ttgctgattt gcagggtact cctgtggagg aggttgaaga aaaagcacct
    15481 cctatcgacc ctgttctttt gcgtccggtg gatgatctgg aattgacagt acgttcagct
    15541 aattgtttga aagctgagga tatttattat attggcgatt tgattcaacg cactgaaacc
    15601 gagcttctta aaacgccgaa tttgggacgt aaatctttga atgagattaa ggaagtattg
    15661 gcatctaaag gtttgacact gggttctaag ttggaagcat ggccacctgt aggcttggaa
    15721 aagccttaat gaagaattaa aggataattg atatgcgtca tcgtaatggc aatcgcaaat
    15781 taaaccgtac cagcagtcat cgtgctgcaa tgctgcgtaa tatggcgaat tcattattga
    15841 ctcacgaaac tattgtaaca actctgccta aggccaagga attgcgccgt gtagtggagc
    15901 cgttgattac attgggtaaa aagccgtcat tggcaaaccg ccgtttggca tttgaccgta
    15961 ctcgcgaccg tgatgttgta gtaaaactgt ttggcgattt gggtcctcgt tttactgctc
    16021 gtaacggtgg ttatgttcgg gtgttgaaat acggattccg taaaggtgat aatgcacctc
    16081 tggcactggt tgaattggtt gacaaaccgg ctgctgagta attttagtca tataacgcca
    16141 tctgccgaaa agcaggtggc gttatttttg caatatctga taggtaatag ggtattggct
    16201 atcatgttta aaatattaat tgaatagcta aggtttgcgc ggtaaactta catcattaaa
    16261 aaattctatg atggtttata taatgaatgc cttcgatata aagtcgacaa agatggacgt
    16321 attgtctata tctttgcata cgtcagactt gtttgatttg gaagatgtgc tggtcaaatt
    16381 gggcaagaag tttcaagagt ctggtgttgt tccatttgtg ctggatgttc aagagtttga
    16441 ttatcccgag tctttggatc ttgctgcatt ggtttcgttg ttttcaaggc atggtatgca
    16501 aattttgggt ctgaagcatt ctaatgaacg ttgggctgct gcggctatga agtatcattt
    16561 gctgttttgt ctgtctcatt cggaaaatgt taaagaactg ggtcaggttg aggtgcagaa
    16621 cacggaggat ggtcagaaag caaggaaaac agtattgatt acatcccctg tccgtaccgg
    16681 tcagcaggtt tatgccgaag atggcgattt gattgttacg ggggcggtca gccagggggc
    16741 ggaattgatt gcggatggca atatacatat ttatgcgccg atgagggggc gtgctttggc
    16801 cggtgccaag ggtgatactt ctgcccgcat atttatccac tccatgcagg cagaactggt
    16861 ttctgtggcg ggtatttacc gtaattttga acaggatttg ccgaaccatc tgcacaagca
    16921 gccggtacag atattgttgc aggataaccg attggttatc agtgcaattg gctcagagta
    16981 attgtttgat atttaaaaag gaaatattgt ggcaaaaatt attgtagtaa cttcaggtaa
    17041 gggcggtgtc ggtaaaacga ctaccagtgc cagtattgcg acaggtttgg cattacgcgg
    17101 atataaaact gcggtaattg attttgatgt gggtttgcgt aacctcgacc tcattatggg
    17161 ttgcgagcgt cgtgtcgttt atgacctgat caatgtcatt cagggggagg cgacgctcaa
    17221 ccaagctttg attaaagata aaaattgtga aaacctgttt attttgccgg cttcccagac
    17281 tcgggataaa gacgctttga cacgcgaggg cgtagaaaaa gtgatgcagg agctgtccgg
    17341 caagaaaatg ggctttgagt atattatttg cgactctcct gccggtattg agcagggtgc
    17401 attgatggcg ttgtattttg ctgatgaagc cattgtaacg accaatcctg aggtttccag
    17461 tgtgcgtgac tccgacagga ttttgggaat tttgcaaagc aaatcccata aggcagagca
    17521 aggcggttcg gttaaagaac atctgttgat tacgcgttat tctcccgaac gagtggcaaa
    17581 aggcgaaatg ctgtctgtac aggatatttg cgatattctg catattcctt tgctgggtgt
    17641 gattcctgaa tcccaaaacg tcttgcaggc atccaattcc ggagaaccag tcatccatca
    17701 ggacagcgtg gcggcttccg aggcatataa ggacgttatt gcccgtcttt tgggcgagaa
    17761 ccgtgaaatg cgtttcttgg aagctgagaa aaaaagcttc ttcaaacgtc tgtttggagg
    17821 ataagatatg tcattaatcg aatttttatt cggcagaaag cagaaaacgg caaccgttgc
    17881 ccgcgaccgc cttcaaatca tcattgccca agagcgcgcc caagaaggtc aggctccgga
    17941 ttacctgccg actttacgta aagagttgat ggaagtcctg tccaaatatg tgaatgtttc
    18001 attagacaat atccgtattt cccaagaaaa gcaggatggt atggatgtgc ttgagttgaa
    18061 cattactttg ccggaacaga aaaaggtata ggacatgacc ttaaccgaat tgcggtacat
    18121 cgtcgcagtc gcccaagaac gtcatttcgg cagggcggcg cggcgttgtt ttgtcagcca
    18181 gcccactttg tctattgcca ttaagaaatt ggaagaagag cttgccgtct ctttgtttga
    18241 ccggagcagc aacgatatta ttacgaccga ggcgggggaa cgtatcgttg cacaggcgcg
    18301 taaggtgttg gaagaggcag agcttatcag gcatttggca aatgaagaac aaaacgagct
    18361 ggagggtgcg ttcaaactcg ggctgatttt tacggttgcg ccgtacctgc tgccgaaact
    18421 gattgtctcg ttgcgccgta ctgcaccgaa aatgcctttg atgttggaag agaattacac
    18481 gcatactttg accgagtcgc tcaaacgcgg ggacgttgat gcgattatcg ttgccgaacc
    18541 gtttcaagag ccgggcattg ttaccgaacc cttgtatgac gaaccgtttt tcgtgattgt
    18601 cccaaaaggg cattcatttg aggaactgga tgccgtttcg ccccggatgc tgggtgagga
    18661 gcaggttttg ctgctgacgg aaggcaactg tatgcgggat caggtactct caagctgttc
    18721 cgaattggcg gcgaaacaac gcatacaggg gttgaccaat acattgcagg gcagctcaat
    18781 caatacaatc cgccatatgg tcgccagcgg tttggcaatc agcgtgttac cggcaaccgc
    18841 gctgaccgaa aacgatcata tgctgttcag cattattccg tttgaaggta cgccgccaag
    18901 ccggcgggtc gtattggcgt accgccgcaa ttttgtccgt ccgaaggcgt tgtcggcgat
    18961 gaaggcggcg attatgcagt cgcagcttca cggggtaagt tttatccgcg actaggcgca
    19021 ggcattgttt tcaaaacgcc atttccctga gccgacaaca cggtatgcca agatattgcc
    19081 gtcatcatcg attttgagta tagcatcgcc acggaaactc cgtcctgaag atattcgact
    19141 tttgcatcac tgtgaatgtt ttcatcagtg ccgatgcaat gccat
//
LOCUS       DAWKOD010000023        18406 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000023 DAWKOD010000000
VERSION     DAWKOD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.023"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..163
                     /locus_tag="WJK73_000610"
     CDS             <1..163
                     /locus_tag="WJK73_000610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557130.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            200..412
                     /locus_tag="WJK73_000611"
     CDS             200..412
                     /locus_tag="WJK73_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ7557131.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WJK73_000612"
     CDS             560..2143
                     /locus_tag="WJK73_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ7557132.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="WJK73_000613"
     CDS             2227..3324
                     /locus_tag="WJK73_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ7557133.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFLWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="WJK73_000614"
     CDS             3438..4553
                     /locus_tag="WJK73_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ7557134.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="WJK73_000615"
     CDS             4678..5133
                     /locus_tag="WJK73_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ7557135.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="WJK73_000616"
     CDS             5204..5680
                     /locus_tag="WJK73_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ7557136.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5668..6594
                     /locus_tag="WJK73_000617"
     CDS             5668..6594
                     /locus_tag="WJK73_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ7557137.1"
                     /translation="MSEMKKNKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6659..7216
                     /locus_tag="WJK73_000618"
     CDS             6659..7216
                     /locus_tag="WJK73_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7557138.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            7565..9256
                     /locus_tag="WJK73_000619"
     CDS             7565..9256
                     /locus_tag="WJK73_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7557139.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            9269..12208
                     /locus_tag="WJK73_000620"
     CDS             9269..12208
                     /locus_tag="WJK73_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ7557140.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYSDNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSPSMTDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(12302..13261)
                     /gene="ttcA"
                     /locus_tag="WJK73_000621"
     CDS             complement(12302..13261)
                     /gene="ttcA"
                     /locus_tag="WJK73_000621"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ7557141.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(13359..14222)
                     /locus_tag="WJK73_000622"
     CDS             complement(13359..14222)
                     /locus_tag="WJK73_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ7557142.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATIKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            complement(14257..14619)
                     /locus_tag="WJK73_000623"
     CDS             complement(14257..14619)
                     /locus_tag="WJK73_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7557143.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKKDAATA"
     gene            14694..15173
                     /gene="ybaK"
                     /locus_tag="WJK73_000624"
     CDS             14694..15173
                     /gene="ybaK"
                     /locus_tag="WJK73_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ7557144.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPRDLNILNPKTIQAAV"
     gene            15196..15918
                     /locus_tag="WJK73_000625"
     CDS             15196..15918
                     /locus_tag="WJK73_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ7557145.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            15920..16795
                     /locus_tag="WJK73_000626"
     CDS             15920..16795
                     /locus_tag="WJK73_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7557146.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(16867..18264)
                     /gene="aspA"
                     /locus_tag="WJK73_000627"
     CDS             complement(16867..18264)
                     /gene="aspA"
                     /locus_tag="WJK73_000627"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ7557147.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVSKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         4759 a         4412 c         4874 g         4361 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agacggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atctgcttga
     1141 agaacccttg gaagcgcgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaaaggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgttaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcaggggat gattatattg ccaaagaatt ttatagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctag aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 cactacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac actgcgaaaa gcggtacacg
     2221 acacatatga accatacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
     2581 gagggattgt ttgcctgttt gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt acattcctcc gatttgagtg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgc
     2761 cctttgcttt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cttgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttccgacggc agccacacgc gccagcgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acaggtttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt tttgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtcttcaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatac cttgcccgct tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgcgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga tagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggtcgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accggaatct gtaaaaaatg tcagaaatga aaaaaaacaa agtccacctg
     5701 atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat ggcgcaaaaa
     5761 gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgagatcgg tattgacggc
     5821 gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg tttgtgttgc
     5881 accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc caagcccgac
     5941 cgcctgatga ttgaagcaag cggactggct cacgcagcca gcgtcatcga cgaactcaaa
     6001 gccaaaccgc tggacagcct tttggaaatc ggcgccgtct ttaccgtcgt caatccgcgc
     6061 cagttcatca accccgatta cgcgcaacag gcgttgtata aagaccaaat cggcatctgc
     6121 gacgtattgg tcgccagcaa aaccgattta tgcaccaccg aacagcttgc cgaatttcac
     6181 gacaaagcgg caaaactgtt cccgcccaaa gccaaagtgg tcgaagtcca aaacgcacaa
     6241 ctcgacatcc ggtggctcga tattcccgtc atcgaaaaat cacgctaccg cctcaaagcc
     6301 ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc cggacgcgat
     6361 ttcgacggcg aaaagctgac caactttttc aacgacctgc ccaaaatgac cgaaggactc
     6421 gtccgcgcca aaggcgtgtt tcaagtattg ggaacgtggg tgtggctcaa ctgggtagac
     6481 ggacagtggg gcgcgaacca agtctcatgg cggcgcgatt cgcgttttga attgattgcc
     6541 aaatcgtttg atgcggattt aatcgaacaa aagctcaaag acgcattgga atagcgttca
     6601 cgcgccttca gacggcatat gaaatgaaaa tgccgtccga aggtctgagg aggacgggat
     6661 gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttccc tgccgtctat
     6721 ggcggcaacc catgttttga tggaaaccga tatgggcaat atccgtttgg ttttggacga
     6781 atccaaagca cccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag gcttttacga
     6841 caatacgatt tttcaccgcg tcatcggcgg cttcgttatc caaggcggcg gattgaccga
     6901 ggacttggca caaaaggcaa gcgataaggc cgttgccaac gaatccggca acggcttgaa
     6961 aaacactgtc ggcaccatcg ccatggcgcg gacggccgat ccggattccg ccaccagcca
     7021 attctttatc aatctggcgg acaatgattc actcaactac aaaaacggac aatacggcta
     7081 taccgttttc ggcagggtcg aaagcggcat gaacaccgtt tccaaaatcg cccgcgtcaa
     7141 aaccgccacg cgcggctttt atcaaaacgt acccgtacag cccgtcaaaa tccgtcgcgt
     7201 tgttgtcggg cagtaacacg cagacagatg ttcagacggc gtcgcccgtt tcacaaaaaa
     7261 cgccgtctga agtgaaaaaa tattttaaaa cagatagttg atattgacaa attcaaaccg
     7321 aggattttaa aatgctgcca acccaaccca acccaaccca acccaagccc ctgatctaat
     7381 cgacacagcc ggggtgaaca ccgaggcaat ctacacttca gacggcatta ccgccaactc
     7441 tacccaactc gaacagctca aaaaactgtt tcccgcctgt tttgacgcag acggaaattt
     7501 ccttatcgac agattacaag ccgaaattgc gccacagacc gacatcggac gcgaatttta
     7561 cgaaatgaac tggctgggca aatcatatgc tcgcctgctt cgcaacctgc cgcccgaaac
     7621 cctgataagt gaagacaaga cgcacaacgc caaacccgaa aatgcaggca gccaaaacct
     7681 actgattcgt ggcgacaatc tagaagtgtt gaaacactta aaaaacgcct acacaaacag
     7741 cgtgaagatg atttacatcg acccgcccta caacaccgga tcagacggct ttgtctatca
     7801 ggacgaccgc aaattcacac cagccgaact tgcctgctta gccaatattg acgaagacga
     7861 agccgcgcgg attttagatt tcaccgacaa aggctcaaac tcgcacagtg catggttgac
     7921 ctttatgtat ccgcgcctgt atatcgcccg cgaactgtta aaggacgacg gcgtgatttt
     7981 tatctcgatt gacgataacg aagcggcgca gttgaagttg ttgtgtgatg aagtgtttgg
     8041 ggaagggaat tttattaaag atttaattgt taatacatca gaaggtggtg gtaatgcaaa
     8101 atatgttgta aatgggcatg aaactgtttt agtttatgcg aaagatatta ctcattttga
     8161 caatctaaaa agaccaaaag atatacgcgg caaaaaaatt gtaatcaatg atgaattata
     8221 ttggatacag gaagatgccg ttcgcgaaca gtttggcaaa tatgggaact tgcattacga
     8281 agatattatt gaaataaaag gaattgagtt taaaaacaaa attgataaag atattgaaaa
     8341 taatgaatat atcttaattc cgaagtctta tgggaaaaca attattggaa aacttagaaa
     8401 agtatccgat gatttttcca aatttcatag catcttaaat attggaaata taagtaaaaa
     8461 tcttactaaa gatggtattc gtgaattaga agagttgttt gaaactggaa aaggttattc
     8521 tccttttgaa acaccaaaac cattagattt attgaaaaga ttagttcttt ctgcaacttt
     8581 caagggaaat caaactgacc taatcctaga cttcttcgca ggcagcggca caaccgccca
     8641 cgccgtgatg cagcttaacg ccgaaggaca aaacggtaac cgccgctata tctgtgtaca
     8701 gcttcccgaa aaaaccgctg aaaaatccga agcccgtaaa gcaggctacc cgaccatctt
     8761 cgacatcacc aaagcccgca tagaaaaagc cgccgccaaa atccgcgtcg aacatcccga
     8821 ttacacgggc gattcgggct tcaaaatctt tcaaacggca gacaatttca ggcagcatcc
     8881 ggacaaggat ttttcgcccg aacaaccgga tttgccgctt aacgatgaat taagcgaaga
     8941 acagctgcaa acgcttctga ccacctggac gctgtatgac ggggcggcac tgaccacgcc
     9001 ggttgagcct gtgcggttag gggcttacac ggcgtatctg tgcgaaaaac ggctgtatct
     9061 gatgaatgcc ggttttactt ccgccgattt gttggcgttt atccgcaagc tggacgacga
     9121 tgcggatttc aatcccaacc gcgtgattgt attcggcagc aatatggcaa gcgccatgca
     9181 gcacgaactt gaccaggcgg ttcgcggtta tgccaataaa aaagagattg agttgaatgt
     9241 ggttatcagg gtttgacgga ggcaattcat gagcggtttt aattacgaga aaaaccagcc
     9301 gcaccaaatg cgggcggttt cggcggtttt gggcgtgttt gacggggcaa cgcccaaata
     9361 tcgggcggca gacgaaaatc ccgaactttt gtttgctgca aaacaatact cagacaatat
     9421 cctgaaagtg caaagccaaa acggcataga cggccgattc cccgaccgtt cggacgacca
     9481 aaatatcctt gatatttcca tggaaacggg cacgggcaaa acctatacct acacacaaac
     9541 catgttcgag ctgcaccgtt ggctgggcgt gttcaaattt atcgtggtcg tgccgacttt
     9601 gtccattaag gcgggaacgc ggcagttttt gcaaagcaag gctttggcag agcattttga
     9661 acaggatttc ggcggcgatt atgaaggcgt acgcctgaaa acctatgtgg tggaaagcgc
     9721 gaaaaagaat aagggcaaaa agtccaatgc gcccataacg atcgagcaat ttgtcaaagc
     9781 ggaaaacaaa aaggaaatcc atgtgctgct gattaacgcg ggcatggtta attcgccgtc
     9841 catgaccgat acgggcgaca aggcattgaa ggatttgttt gacaatcccg ttgatgcatt
     9901 ggctgccgtg cgcccgtttg tgattgtgga cgaaccgcat aaattcccga cccgggagag
     9961 cgcgaaaacg tggggcaata tcaaacgctt aaaaccgcaa tatattttgc gctacggtgc
    10021 gacatttaac gatgaatatt acaacctgct ttaccgtctg acggctgtag acgcgtttaa
    10081 cgacgggctg gtcaaaggcg tgcgcgtgtt tcaggaagaa atgcagggcg gcatggatgc
    10141 ggcggtaaaa ctggtgtcgt cggacggcaa agaagcgaaa tttgaattaa acgaaaagga
    10201 caaaaagcag acgttcaaac tggcaaaagg cgaagatttg gcgaaaatcc atcccgctgt
    10261 ttcggatttg aaaatcgaca aaatgaataa aaccgtggtg gtgttaagta acggcttgga
    10321 gttgaaaacg ggtgccgtca tcaaccctta ttcctattcg caaacggtgc aggatgcgat
    10381 gatgcagcgg gcggttgccg agcatttcaa gctggaacgt gcgcttttgg cagaacgcgc
    10441 gccacagccc aaaatcaagc cgctgacact gttttttatt gacgatattg cgggctaccg
    10501 cagcggcaac gagctttcag gcagcctgaa agataaattt gaaagctgga ttcacgcgga
    10561 agcggcacgc cgcctgaaaa cggaaagcga cccgttttac cgcgattacc tgcaaaagac
    10621 gttggacgat gtatccgcct gccacggcgg ttatttttcc aaagacaata cagacagcga
    10681 cgatagaatc gagcaggaaa tcaatgaaat cctgcacgat aaggaaaaac tgttgtcttt
    10741 ggacaacccg cgccgcttta ttttttccaa atggacgctg cgcgaaggct gggacaatcc
    10801 caacgttttc cagatttgca aactgcgttc cagcggcagc acgacttcca agctgcaaga
    10861 agtcggacgc ggcctgcgcc tgccggtaaa cgagctgatg gcgcgggtgc gcgatgtgcc
    10921 gtacaaactg aattattttg tcgatagcag cgaaaaagac tttgtgaagc agcttgtcgg
    10981 cgaaatcaac gataattctt ttcaggaaga aatctccaaa aagtttaccg aagagctgaa
    11041 acaaaaaata ttgcaaaaat accccgatat caaaccgctg gtattggtaa accaactgtt
    11101 ttcagacggc atcattgacg acaatgaaaa ctttaccaaa gacggctatg acaaattaaa
    11161 agccgcctat cccgaagcct tccccaaagg tttggacaaa ggcaaagtca gcaacgccaa
    11221 agacgagggc aaagacacca tcatcatgcg cgaaggcaaa tatgaagaac tcaaggcctt
    11281 gtgggagctg attcaccata aagccgtttt gcagtacaaa atcaaggatg aagccgaatt
    11341 tgtcgattta tttaccgcct atttgcgtga aaacgccgcc aaattcccgc aggcaggcat
    11401 acgcacggcg gtaaacgaag cttatatcaa caacgggctt atgctttccc gccgcataga
    11461 cagtattgaa gatgaagatt ttatccgttt caacacaatg acttaccgag agtttctgga
    11521 aaaactggca caaacggcaa aaatccgtat gcagactttg catcaggcgt tttaccgcat
    11581 ccgcaacgaa ctgaacattg gcgatttttt gaatatgcaa accatcgccc aaatcaaaaa
    11641 cggcttcaac cggtttttgc ttcatcattc cttccataaa ttcgaactgg attaccggct
    11701 tgtcggcagc aaaatccatc cgaccaaatt taccaataaa gacggcaaac cgcgcgcggt
    11761 gaaaaaagca gatttgggca gatttgaaga tacggagcac cggcctgccg ccggctatct
    11821 cttcgacgag attttctacg attcggatat agaacatgaa aatgtcgcca acaaccaaat
    11881 tgaaggcgta accgtattta ccaaaatacc gagaaactcc atcaaaatcc ctgttgccgg
    11941 cggcggcacg tattcgcccg attttgccta tatcgtgaaa accaaaagcg gcgagatcct
    12001 gaactttgtg attgaagcca aagggactga cggggcggaa gatttgcgaa aaagcgaaga
    12061 gcggaaaatc aaacatgccg aaaagctgtt tgccgagatt tccaaagaaa tcaaggtggt
    12121 gttcaaaacg cagtttgacg gcgagaggat agccgaactg atcgggcaaa atatgcccgc
    12181 aggcgggcat tctgaaaacg gacactgaga aacaaatgtt tggaagccgt ctgcttcggg
    12241 attttaaaat gccgtctgaa acgccaaacg ggcttcagac ggcatttttg acggcggggg
    12301 gttacgcgcc gcaggttttc ggcttgttcg ccagaatgtt gatgactttg cgttcggctt
    12361 tttgcggcgc gattttgatt tcgctttcgt cttcttcgct gccgtctgaa aaacgttcgg
    12421 gcattttttc gctgtcaaac gccaaatcgc cgccgtgttt cagggtttga ccgcgttcca
    12481 atccggcaaa gtcgaagagt tcggtatcgg caaggtggga agggacgacg ttttgcaggg
    12541 cggagaacat cgattcgatg cggccgggaa agcgtttgtc ccaatcgcgc agcatatcgc
    12601 cgatgacttg gcgttgcagg ttgggttgcg agccgcagag gttgcacggg atgatgggga
    12661 attgttttaa ttcggcgtat ttgattaagt ctttttcttt tacatacgcc agcggacgga
    12721 tgacgatgtg ttcgccgttg tcgctcacca gcttgggcgg catggctttg agtttgccgc
    12781 cgtaaaacat atttaaaaac aaggtggcga ggatgtcgtc gcggtggtgt cccaaggcga
    12841 ttttggtgca gcccaattct tttgcagtgc ggtagaggat gccgcggcgc aggcggctgc
    12901 acagcgaaca agtcgttttg ccttcgtcta atacgcgttt gacggtggag taggtgtctt
    12961 cttcaacaat tttgtaggga acgccgatgc tttcgagata ggtcggtaat acttcttcgg
    13021 ggaagcccgg ctgcttttgg tcgagattga cggcaaccag ttggaaatca atcggcgcgc
    13081 tggcttggag ctggcgcagg atgtccaaga gggcatagct gtcttttccg ccggagaggc
    13141 agaccatgat tttgtcgccc ggctcgatca tgttgaaatc gttaatcgcg tcgccgacgg
    13201 cgtggcgcag gcgtttgctg agtttgttgt tttccagttc ttgtttggtt tttttggaca
    13261 tggcggtggt ttgaaaaatt ggggatgcgg cattgtaacc gaaaacaggg gcggttttaa
    13321 aatgccgtct gaaaaaggtt cagacggcat tagagggatt attctgcatt ttcggtttta
    13381 aagaagggat gaaccgcttt gaagatactg ccgtttgggg cggtcagttc tttttgcgcg
    13441 gcggagaatt taatcacggc aagggcggta aagttttccg aatcttgaac gctgtcgagt
    13501 acgatgccgg tctcttcgcc gtctgccgtc agcagggttc ctgcttcgac ggccgaattt
    13561 cccgacaata ccgccaagcc gcgtttgacc tgaccgcgat actgggcacg ggcaatgatt
    13621 tcctgtcccg gatagcagcc ttttttgaag tgtacgccgc cgatgatgtg ctggttgagc
    13681 atttgggcga cggcggtttc tttggtagcc gcgcatatcc acgggtaacc gctgcggatt
    13741 tcgtgcagcc gccacgcgtt ttcggcggcg gcatcataag gcggcaaggc gtttttgggg
    13801 gcgatgtgca aaatgccccg atggggcagg acgacggtgc agatgccgtc tgaaacgcat
    13861 tcggcggtaa agacgagact gggttcttgc gcggcaagcg gttcggcgga tgcttccaat
    13921 tccgcgtcga cggcgtaatc ttcaaggatt tcaaaaacga ctttagcgcg caggacaaac
    13981 atccgcaggc gtttgatcgt tgcttcaagc aagtcttgcg ccataatcag cagcaaatcg
    14041 ccgcctcggt tgacgacaat catattggcg atgacgcggc ctttgggcgt gttgtaagtc
    14101 gcataacacg cctgcccggt ctgaagatta ttgatgtcgt tggaaagctg tccgtgcagg
    14161 aaggtttggc ggtcttcgcc gctgacgcgt accacgccga aaaagggcag taaggttttc
    14221 atcatttgcg tactctgaaa tataaaggaa atctgtttat gcggttgccg catctttctt
    14281 cacggcggtt attttgattt cgacgcacca ctccggacgg gcgagccgag cttccacgca
    14341 ggcgcgggcg ggcgttctgc cggcggcaac ccaagcgtcc cacacgccgt tcatttccgc
    14401 atagtcgccc atatcgcgca gatagatgac cgcatccaaa acgtgcgctt tgtccgagcc
    14461 gcattccgcc agccagcggt cgatttgggc aagcacgtcg gcagtctgtt cggcagccgt
    14521 ttcaccgttt tcgggaacca tgccggagag gaaaatcaag ccgtttgcgc cgacggcttc
    14581 ggaatagcgg ggcgttgtgc cgaaatatcg gatatccata tcggtttcct tcgataaagg
    14641 ggatatatgg taacattgcg cttgaccgat ttccatgttt tgcatgacga aaaatgagta
    14701 aacacactta tccgataaca cctgccgtgc gcgttttgcg tgaaaacggc atcgaatttg
    14761 aaccttttac ctatgcctat gaggaacacg gcggcacggc gcagtttgcc cgactattcg
    14821 gcaaagacga acacttggtc attaaaacca ttgttttgca agatgaaaac ggtaaggggc
    14881 tgattgtctt gatgcacggc gacaagcaga tttcaacccg caatctggcg cggcatttgg
    14941 gtgcgaaaca cattgaaccc gccacgcccg tacaggcaaa caagtggacg ggctatctgg
    15001 tcggcggcac aacgccgttc ggcatccgga caaagttgga tatttacgtc gaacagtcgg
    15061 tgatggattt ggaaaccatc tatatcaacg gcggaaaacg cgggttcatt atcggcatcc
    15121 gtcccagaga tttaaatatt ttgaacccga aaacaataca ggcggcggtt tgacgggaaa
    15181 gtataaagga acaatatgga caaagatttg tatgccgtat tgggcgtgtc gccgcaggcg
    15241 ggagcggacg aaatcaaacg cgcctaccgc aagctggcga tgaaatatca tcccgaccgc
    15301 aatccgggca atccgaaggc ggaagaaaag ttcaaagaaa tccaacgggc ttacgatacg
    15361 ctttccgacc tgtcgaaacg gatgcaatac gacgcgtcct tcagacggca tgaggaacgc
    15421 gggcggcagg aagaggcatt ccgccgcgaa caggcgcgca gggagcagtt ttaccgcgaa
    15481 cagatgcgcc gcgaacaggc gttcagacag gcgtttgaac ggcaggcatc acgttcgtgc
    15541 catacttacg aaccgtccgg cggcggaagc gggcgcaact atgtcctcgc cgcctacatc
    15601 ctgttcggtt tgggtgcaat catgctgttc atgcccatag tcggcgtgat tttcgcctat
    15661 atgcccatag tcggcgtgat tctcgcctat atgaaacgga acagtttgga cagcattgtc
    15721 tatgccgcac ataccgaata cctgattaaa accttttggc gcacattttg gctttatatt
    15781 ttgggtgcgc tgactgccct tttgggtatc ggcgtgctga ttattattgc aacgaacgtc
    15841 tggtatttct accgcatcat cgccggcttt atccgcttca acggcggcag ggcggttgca
    15901 cccgagaaat ggatatagta tggcttacct gttaatcagc atcgtgttca gcgtgtcggt
    15961 ttccattttg ctgaaaatgg caaggaagaa aaaaatcgac atcgcgcagg cggtcgccgt
    16021 caattatgtg gtcgcggtca tactgaccct gctggtattg aagccggatg tcggcaatat
    16081 cggcgcattt ttgccgacat ggtggctgtt tgccgcattg ggcgtgctgc tgccgtccgt
    16141 atttgtcatt atgggcaaat ccgtagaatc cgccggtatc gtcaaatccg acgcggcgca
    16201 gcgtttgtcg ctgtttttgc cgattgttgc cgccttgacg ctgtttggcg aaaaactcag
    16261 cgaaggcaga ctaatcgggc tgtgcctcgc atttgccgca ctgttctgcc tgctttggaa
    16321 acacagcggt ggcaaaaaat caggaagcgc gtggcggcag gcggtattgc tgctgggcgt
    16381 gtgggcaggt tacggcatta tcgatatcct gttcaaacag cttgccaaaa gcggcacggc
    16441 atttgcgggc aacctgctgg ttgcatttgt gctggcgggt gtgctgatgt ttgcctgcct
    16501 gtttgccaaa tcggtcagat ggcgtgttga gagtgtggtt ggcggcatat tcttgggcgg
    16561 tttgaacttt atgaacatcg taacctacat caccgcgcac caaatgatga aggataatcc
    16621 gaccttggtt tttgccggta tgaatatcgg cgtgattgtt ttgggtacgc tttcgggcgc
    16681 attgttcttt aaggaaaaaa tcaacacaat caatacggcg ggaatcgtgt tggcactgtg
    16741 ttctatcgcc tgcctgtttt attgggggga agtcaaggta ctgttcggca tataatccgg
    16801 ctgattgata taacaaaatg ccgtctgaag cagcatccct gcttcagacg gcatttgtct
    16861 gcaacgttac agatgggggt tcatcaggtt ttcaggagag aggatgcggt tgagttcttc
    16921 ttcgctcaac agcccgcgtt ccaagacaac ctcgcgcacg cctttgccgg tttgggcgca
    16981 gattttgctg accaaatcgc cgttgtggtg gccgatatac ggattcagat aagtaaccag
    17041 cccgatggag ttgaaaacgt aacgctcgca tatttcgcgg ttgaccgtaa tgcctttgac
    17101 gcatttgtcg gacaggttga ttgctgcatt gcccaagagg gaaatggttt caaacataca
    17161 ttgggcgatg accggctcca tcacgtttaa ttgcagttgc ccggcttcgg cggcgaaggt
    17221 aatcgtcgta tcgttgccga tgactttgaa gcagacttgg ttgaccactt cgggaatcac
    17281 gggattgact ttggcaggca taatggaaga accggcctgt aattcgggca ggttgatttc
    17341 tttcaagccg gcacgcgggc cggaagagag caggcgcaag tcgttgcaga ttttggagag
    17401 tttgaccgcc gtgcgcttca atgcgccgtg taccatcaca tatgcgccgc agtcggaggt
    17461 cgcctcaatc aggttttcgg tcagtttgca aggcaagccg ctgacttcgg agagtttttt
    17521 gaccaccagt tcggcgtagc ctttgggcgt gttcacgccc gtgccgattg ccgttgcgcc
    17581 caaattgact tctaaaagca gttggcgcgt gcggtcgagg ttgaggattt cttcttccaa
    17641 caacacttgg aaagattgga attcttgtcc tgtagtcatt ggcacggcat cttgaagctg
    17701 ggtgcggccc attttcaaaa cgtctttaaa ttcttcggct ttggcggcaa aggcgttttt
    17761 cagaacgacc agtttgtcga gcaattcgcc gatactgtaa tacacggcaa ggcggaagcc
    17821 ggtagggtag gcatcgttgg tggattggct ggcgttgacg tgatccatcg gattgacgat
    17881 gtcgtagcgg cctttttcgt atcccaaggc ttccaatgcg aggttggcga tgacttcgtt
    17941 ggtgttcata ttaaccgaag ttcccgcacc gccctgatac acgtcggagg ggaattggtc
    18001 gaggcagcgg ttgttcagca gaacttcgtc gcaagccttt tcaatggcgg cggcgatttc
    18061 gggttttact gcacccaatt cgccgtttgc ctgcgcggtt gcttttttca ccatcaccat
    18121 actgcggaca aactgcggca cgtcggaaat tttttgtgtg gagattttaa agttttcaat
    18181 ggcgcgcaga gtgtggatgc cccaatacac ttcggcggga atctcgcggt cgcccaataa
    18241 atcgtgttcg atacggacag tcatgtttta cctttgtaag tcggataatt aatattgaaa
    18301 aaatgcgcca tcggaaagat gccgccgcag gacgaacact ataccggccg gatgaaattg
    18361 tccatatcgt atgccgtctg aaaacgggaa acgttgtttt cgggtg
//
LOCUS       DAWKOD010000024        18282 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000024 DAWKOD010000000
VERSION     DAWKOD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.024"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..1818
                     /gene="pgi"
                     /locus_tag="WJK73_000628"
     CDS             175..1818
                     /gene="pgi"
                     /locus_tag="WJK73_000628"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7557148.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            1788..2018
                     /locus_tag="WJK73_000629"
     CDS             1788..2018
                     /locus_tag="WJK73_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557149.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILPENTGKRPTKHLT"
     gene            2028..2849
                     /gene="dapD"
                     /locus_tag="WJK73_000630"
     CDS             2028..2849
                     /gene="dapD"
                     /locus_tag="WJK73_000630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ7557150.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            3004..3789
                     /gene="fabI"
                     /locus_tag="WJK73_000631"
     CDS             3004..3789
                     /gene="fabI"
                     /locus_tag="WJK73_000631"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ7557151.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4033..5031)
                     /gene="ilvE"
                     /locus_tag="WJK73_000632"
     CDS             complement(4033..5031)
                     /gene="ilvE"
                     /locus_tag="WJK73_000632"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ7557152.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            5341..5655
                     /locus_tag="WJK73_000633"
     CDS             5341..5655
                     /locus_tag="WJK73_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ7557153.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            5666..6835
                     /gene="lapB"
                     /locus_tag="WJK73_000634"
     CDS             5666..6835
                     /gene="lapB"
                     /locus_tag="WJK73_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ7557154.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            6860..7276
                     /gene="gloA"
                     /locus_tag="WJK73_000635"
     CDS             6860..7276
                     /gene="gloA"
                     /locus_tag="WJK73_000635"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ7557155.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(7449..9989)
                     /locus_tag="WJK73_000636"
     CDS             complement(7449..9989)
                     /locus_tag="WJK73_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ7557156.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVAETSD
                     FHTAADDLSALLQPAEAPAVEENVTKTVAEIPDFNATADDLSALLQPSEVPAVEENAA
                     EITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAP
                     SEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            10297..10827
                     /locus_tag="WJK73_000637"
     CDS             10297..10827
                     /locus_tag="WJK73_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ7557157.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            10830..11120
                     /locus_tag="WJK73_000638"
     CDS             10830..11120
                     /locus_tag="WJK73_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ7557158.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            11120..11398
                     /locus_tag="WJK73_000639"
     CDS             11120..11398
                     /locus_tag="WJK73_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7557159.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            11447..12313
                     /locus_tag="WJK73_000640"
     CDS             11447..12313
                     /locus_tag="WJK73_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7557160.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            12384..13142
                     /locus_tag="WJK73_000641"
     CDS             12384..13142
                     /locus_tag="WJK73_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7557161.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            13216..13707
                     /locus_tag="WJK73_000642"
     CDS             13216..13707
                     /locus_tag="WJK73_000642"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ7557162.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            13738..14748
                     /gene="dusA"
                     /locus_tag="WJK73_000643"
     CDS             13738..14748
                     /gene="dusA"
                     /locus_tag="WJK73_000643"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ7557163.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(14818..15705)
                     /gene="gluQRS"
                     /locus_tag="WJK73_000644"
     CDS             complement(14818..15705)
                     /gene="gluQRS"
                     /locus_tag="WJK73_000644"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ7557164.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(15722..16012)
                     /locus_tag="WJK73_000645"
     CDS             complement(15722..16012)
                     /locus_tag="WJK73_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557165.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(16166..17221)
                     /gene="tal"
                     /locus_tag="WJK73_000646"
     CDS             complement(16166..17221)
                     /gene="tal"
                     /locus_tag="WJK73_000646"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ7557166.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPNATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            17317..>18282
                     /locus_tag="WJK73_000647"
     CDS             17317..>18282
                     /locus_tag="WJK73_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ7557167.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         4025 a         4736 c         5288 g         4233 t
ORIGIN      
        1 tgcggctcgc cgccttgtcc tgatttttgt taattcacta tattttcgcc atatggaaat
       61 aaggtgctat tggacgcggc gggcggtgtt ccggagattc gccaaagccg ctgccgtttg
      121 ttaaactaca ttctgctaca ttttaatccg gttctgaaaa atcaaggaaa acagatgaat
      181 gcttttaccc gtgcatggta tgcgctcgaa cgccattatc aggatacgcg tcatgtcctt
      241 ttgcgcgacc gctttgcctg cgaacccgac cgttttgagc gtatgcatga gcgtttggac
      301 gggatgttgt tcgattacag caaaaaccgt ttgggcgaag atacgctgca actgctctgc
      361 cgtcttgcgg agacggcgga tttggaaggg aaaatgcgtg ctttgcggac gggtgcgaaa
      421 gtcaacggca gcgaggggcg tgccgcgctg catacggctt tgcgcctacc cgacggtgcg
      481 gatgccgttt atgcggacgg cagggacgtg ttgcccgaaa tccgccgcga gttaaatcgt
      541 gcgttggagt ttgcacacag tttggacgac ggttcgtatc aggggacaac cggaaaacgg
      601 attacggatt ttgtccacat cggcataggc ggatccgacc tcgggccggc aatgtgcgtg
      661 caggcacttg agccgttcag acggcatatc accgtccatt ttgccgccaa cgccgatcct
      721 gcctgcctgg atgcggtttt atgccgtctg aaccccgaaa cgacagtgtt ttgcgttgcc
      781 agcaagtcct tcaaaacacc ggaaaccctg ctcaatgcac aggcagtcaa ggcgtggtat
      841 cgcggtgcgg ggttctcgga atccgaaacg gcgtgccatt tttgcgcggt gtctgccgac
      901 actgcggcag ctgcggcttt tggtatcgcg gcggaacgcg tgtttgcgat gtacgactgg
      961 gtgggcggac gctattccgt ctggtcgccc gtcggtttgc ccgtgatggt tgcggtcggc
     1021 ggggcgcgtt tccgcgagtt gttggcgggg gcgcacgcga tggacagcca ttttttccac
     1081 acgccgccgc gccgcaacat tcccgttctg atggcactga ttgccgtgtg gtataacaat
     1141 ttccaacacg cggacgggca gaccgccgtt ccgtacagcc acaacctgcg cctgctgccg
     1201 gcgtggctga accagctcga tatggagagt ttgggcaaaa gccgcgcttc agacggcagt
     1261 cccgccgcgt gcaaaacggg cggcatcgtg ttcggcggtg aaggggtcaa ctgccagcac
     1321 gcctatttcc aactgctcca ccaaggcacg cgcctgattc cctgcgactt tatcgtcccg
     1381 gtgacggcgc agggcgtgga ggacggacgc agccgtttta ccgttgccaa cgcctttgcc
     1441 caagcggaag ccctgatgaa gggcaaaacc ttggacgaag cacgcgccga actggcagat
     1501 ttgcccgaag cggaacgcga acgcctcgcg ccgcataaag agttccccgg caaccgcccc
     1561 agcaacagca tcctgctcga ccgcctcacg ccctacaatc tgggtatgct gatggcggct
     1621 tacgaacaca aaaccttcgt ccaaggcgcg atatggaacg tcaacccctt cgatcagtgg
     1681 ggggttgagt acggcaaaca gttggcgaaa accatcatcg gcgaactgga aggcggcacg
     1741 tccgtacacg atgcctcgac cgaagggctg atggcgtttt accgcgaatg ccgtctgaaa
     1801 ggcggcggcg cggcataaaa gtgccgccgc cgttctgtat tgattcgggt gcggaaaagg
     1861 caatacctgc cgcccgcccg attccgaaac gccaatgttc ggcaaccgcc cgcgtattgc
     1921 tgacgaatat gcgtttgcgt ggcacaatag cgcattcatt tcaaatgaac atactgcccg
     1981 aaaataccgg caaacgtccc acgaaacatc tcacataagg aaacattatg tctttgcaaa
     2041 acatcatcga aaccgccttt gaaaaccgcg cggacatcac cccgaccacc gttactcccg
     2101 aagtcaaaga agccgtgttg gaaaccatcc gccagctcga ttccggcaaa ttgcgcgtcg
     2161 ccgaacgctt gggcgtgggc gagtggaaag tcaacgaatg ggcgaaaaaa gccgtgttgc
     2221 tgtccttccg catccaagac aacgaagtcc tcaacgacgg cgtgaacaaa tacttcgaca
     2281 aagtgccgac caagtttgcc gattggtctg aagacgagtt caaaaacgca ggcttccgcg
     2341 ccgttccggg tgcggttgcc cgacgcggca gctttgttgc caaaaatgtc gtgctgatgc
     2401 cgtcttatgt caacatcggc gcatacgttg acgaaggcgc gatggttgat acttgggcga
     2461 ccgtcggctc ttgcgcgcaa atcggtaaaa acgtccactt gagcggcggt gtcggcatcg
     2521 gtggcgtact cgaacccctg caagccagcc cgaccattat tgaagacaac tgcttcatcg
     2581 gtgcgcgttc tgaaatcgtt gagggcgtga ttgtcgaaga aggcagcgtg atttcgatgg
     2641 gcgtgttcat cggtcaatcc accaaaatct tcgaccgcac caccggcgaa atctaccaag
     2701 gccgcgtacc ggcaggttcg gtcgtcgtgt ccggcagtat gccttccaaa gacggcagcc
     2761 acagccttta ctgcgccgtc atcgtcaaac gcgtggacgc gcaaacccgt gcgaaaacca
     2821 gcgtgaacga attgttgcgc ggcatctgat gccttaaacc gtatttgaaa cgtccgatgc
     2881 cgtctgaaat ccgcttcgga cggcattgcc gtttgcatgc tgcaacgtga aaacacagaa
     2941 acagggacaa tttgctataa tcaacggttt agaatgaacc gaacactatt tgaaggatac
     3001 aaaatgggtt ttctgcaagg caaaaaaatt ctgattaccg gcatgatttc cgagcgttcc
     3061 atcgcttatg gcatcgccaa agcctgccgc gaacaaggcg cggaactggc gtttacctac
     3121 gttgtggaca aactggaaga gcgcgtccgc aaaatggcgg cggaattgga ttccgaactc
     3181 gtattccgct gcgatgtcgc cagcgacgac gaaatcaacc aagtgttcgc cgacttgggc
     3241 aaacattggg acggcttgga cggtttggtg cattccatcg gttttgcgcc gaaagaagcc
     3301 ttgagcggcg acttcctcga cagcatcagc cgcgaagcgt tcaacaccgc acacgaaatt
     3361 tccgcataca gcctgcccgc gctggcaaaa gccgcccgtc cgatgatgcg cggcagaaat
     3421 tccgccatcg tcgccctgag ctacttgggc gcggtacgcg cgattccgaa ttacaacgtg
     3481 atgggtatgg caaaagccag ccttgaggca ggcatccgct ttaccgccgc ctgtctgggc
     3541 aaagaaggca tccgctgcaa cggcatttcc gcaggtccga tcaaaacgct cgccgcctcc
     3601 ggcattgccg atttcggcaa actcttggga cacgtcgccg cccacaatcc gctgcgccgc
     3661 aacgttacca tcgaagaagt cggcaatacc gccgccttcc tgctgtccga cctgtcgtcc
     3721 ggcattaccg gcgaaatcac ttatgttgac ggcggttaca gcatcaatgc cttgagcacc
     3781 gagggataat ccgccgtttt caaatccgtg cgccgtccgt gccgcatatc ggtttcgggc
     3841 ggcgttttgc cgtctgaagc gtatttctag ggaaatgccc gacttacggc aggcgggatg
     3901 ggaaatgcgg acgcttgttt taaccgattg cctttgtgcc gacttgctgc aggtgcagcg
     3961 gaaacggttc ggatgcgaaa atgccgtctg aaacgccaaa cgggtttcag acggcatttt
     4021 ttatttaaag catcagcaca cttcaaccag ccagccgtat ttgtcttccg ccaaaccata
     4081 ctggatgtcg gtaatcgcct tacggatggc atagccgcgt tcttggcttt tcacttcgat
     4141 ttctttgccg ccgatgacga aggaagtaac gggcgagatg acggctgccg taccggtcag
     4201 aatcgcctcc gcaccgtttt ccaccgcagc tttgagttcg tcaaccgtga aattgcgttc
     4261 gctgacggta tagcccaaat ctttggcaac cgtcagtacg gaatcgcggg ttacgccgtg
     4321 caaaaattcg tcggtcagcg gtttggtaat gatttcatcg ccgttaatca ggataaagtt
     4381 ggacgcgccg gtttcctgca cgtcgccgtt cgggcagaag aggacttggt ttgcgccgta
     4441 ttcggctttc gccttcagca cccagtgcat ggcggaagcg tagttgccgc cgcatttgac
     4501 gcggcccata tgcggggcgc agcggatgtg ttcggtttcc actaaaattt tgacgggcga
     4561 gccgactttg aaatagtcgc cgacggggga agccaaaata tacagcaggg cggtttcgga
     4621 aggagaaccg gccttgccga tgacgggatc ggtaccgatt aaggtcgggc gcaggtacag
     4681 ggcggcaggc gcatcgggaa tttcgtcggc ggcacgtttg accaattcga ttagcgcgtc
     4741 gagataggct tgggtttcgg ggcggggcag gtgcaaaatg tccgcacttt gccgcatacg
     4801 cgcgatattg gcagtcggac ggaacagtac gattttaccg tctgcctgac ggaaggcttt
     4861 cagtccctcg aaacattcgc tgccgtagtg cagggcgtgc gcacccggta cgagggagag
     4921 gtcttgggaa gattgccatt cggtcggctg ccatttgcct tcgcggtagg cgaggacggg
     4981 catttgactg tgaaaaacgc tgccgaatac ggcgggtacg ggtctgctca tgatgtaaag
     5041 cctttcttat tctgatatgt ttcaatgaac ggtttgaatt tgaagattgt aaagatacgc
     5101 ctgcaaacag ggttttgaca agtgcgcggc gggtttttct gtcgatgcgg tgtccaatcc
     5161 gttatttttc aaatggaaag gaacggtgta tttggtaaaa ttgtcggcaa tcgcatactc
     5221 cgtatgtcgt ccgaacacgc tgccgcatcc tatccgaaac cgtgcaaatc gtttaaacta
     5281 gcgcaatctt ggttcagagt gcgaagctgt ccgggcggcg tttttattta cggagcaaac
     5341 atgaaactta tctataccgt catcaaaatc attatcctgc tgctcttcct gctgcttgct
     5401 gtcattaata cggatgccgt taccttttcc tacctgccgg ggcaaaaatt cgatttgccg
     5461 ctgattgtcg tattgttcgg cgcatttgta gtcggtattg tttttggaat gtttgccttg
     5521 ttcggacggt tgttgtcgtt acgtggcgag aacggcaggt tgcgtgccga agtaaagaaa
     5581 aatgcgcgtt tgacggggaa ggagctgacc gcaccaccgg cgcaaaatgc gcccgaatct
     5641 gccaaacagc cttgagaaag ccgatatgga caatgaattg tggattatcc tgctgccgat
     5701 tatccttttg cccgttttct tcgcgatggg ctggtttgcc gcccgcgtgg atatgaagac
     5761 tgtattaaag caggcaaaaa gcataccgtc gggattttat aaaagtctgg atgccttggt
     5821 tgaccgcaac agcggacgtg ccgccaggga attggcagag gtcatcgatc agcagcctca
     5881 gtcatacgac ctgaacctca ccctaggcaa gctttaccgt cagcgtggcg aaaacgacaa
     5941 agccatcaat atgcaccaaa cattgcttga ctctcccgat acaaccggag ccaagcgcgc
     6001 gcgcgtcctg tttgaattgg cgcaaaacta ccaaagtgcg gggttggtcg atcgtgccga
     6061 acagattttt ttggggctgc aagacggtaa aatggcgcgt gaagccagac agcacctgct
     6121 caatatctac caacaggaca gggattggga aaaagcggtt gaaaccgccc ggctgctcag
     6181 ccatgacgat cagacctatc agtttgaaat cgcccagttt tattgcgaac ttgcccaagc
     6241 cgcgctgttc aagtccaatt tcgatgtcgc gcgtttcaat gtcggcaagg cactcgaagc
     6301 caacaaaaaa tgcacccgcg ccaacatgat tttgggcgac atcgaacacc gacaaggcaa
     6361 tttccctgcc gccgtcgaag cctatgccgc catcgagcag caaaaccatg catacttgag
     6421 catggtcggc gagaagcttt acgaagccta tgccgcgcag ggaaaacctg aagaaggctt
     6481 gaaccgtctg acaggatata tgcagacgtt tcccgaactt gacctgatca atgtcgtgta
     6541 cgagaaatcc ctgctgctta agtgcgagaa agaagccgcg caaaccgccg tcgagcttgt
     6601 ccgccgcaag cccgacctta acggcgtgta ccgcctgctc ggtttgaaac tcagcgatat
     6661 gaatccggct tggaaagccg atgccgacat gatgcgttcg gttatcggac ggcagctaca
     6721 gcgcagcgtg atgtaccgtt gccgcaactg ccacttcaaa tcccaagtct ttttctggca
     6781 ctgccccgcc tgcaacaaat ggcagacgtt taccccgaat aaaatcgaag tttaaccacc
     6841 accgaaagga acacaaaaaa tgcgcttact ccatactatg ctccgcgtgg gcaatctcga
     6901 aaaatccctc gatttctacc aaaacgtttt gggtatgaaa ctgctccgcc gaaaagatta
     6961 tcccgaaggc agatttaccc ttgccttcgt cggttacggc gatgaaaccg acagcacggt
     7021 tttggaactg acgcataact gggatacgga acgatacgac ttgggcaacg cctacggaca
     7081 catcgcggtt gaagtggacg atgcctacga agcctgcgaa cgtgtgaagc ggcagggcgg
     7141 aaacgtcgtc cgcgaagccg gcccgatgaa acacggcaca accgtgatag ccttcgtcga
     7201 agaccccgac ggatacaaaa tcgagttcat tcaaaagaaa agcggcgacg attcggttgc
     7261 ctatcaaact gcctgatacc gccgccgcca atgccgtctg aagcctttag ggggttcaga
     7321 cggcattttg ttgtcggcgg tctgctgttt gagcctgtgc cgggtcaaac tttatccgtt
     7381 acaccgataa ggcaaaaaaa gatgccgtct gaaacggcat ccttgatctg cgaaagggca
     7441 gttgggaatc aaatacccaa ttcctgcgcc aatgcttggg cacgtttgag tacgtcgcct
     7501 tccgcttctt ccagcaattt ctgcactgtc tcggcagcgg catcgcggtc gccgatttcg
     7561 agatacattt cggcaaggtc gtatttcgct tcggaaggcg cgtcagaacc tacagattcc
     7621 gaagggaaac tggtatctgc attatttggg atattttctt ccgagaggta gatgctccaa
     7681 tctaccgttt cctcctcgcc gtctttcagg aagtcgggca aagcgtctgc ctcagaggtg
     7741 ttggaatcag gcgtttccaa agtgatttcc gctgcatttt cctcaacggc cggtacttca
     7801 gaaggttgaa gtaatgcgga caaatcgtct gcggtggcgt tgaaatcagg tatttcggca
     7861 acggttttcg ttacattttc ctcaacggcc ggtgcttcag caggttgcaa cagtgcggac
     7921 aaatcgtctg cggcggtgtg gaagtcggac gtttcggcaa cagtttccgt tacattttcc
     7981 tcaacagccg gtgcttcagc aggttgcaac agtgcggaca aatcatcggc aacgatttcc
     8041 gctgcatttt cctcaacggc aggtacttca gaaggttgaa gtaatgcgga caaatcgtct
     8101 gcggtggcgt tgaagtcggg tgtttcggca acggtttccg ttatattttc ctcaacggac
     8161 ggtgcttcgg caggttgaag caatgcggac aaatcgtctg cggcggcgtt gaaatcgggc
     8221 gtttcaggcg cagtttccgc gacggcatcg gtttcgtaca ctttcaggaa atcgtgcaac
     8281 tcttccggtg tttggacttc ggcaactgtt ttttccaaga tggtttcggg cgaggaagcc
     8341 ttcaggaagc ctgccagtcc ggagggtgag gcaggttttg cggaagctgt ttcttctgtg
     8401 ccgatatggt tgtttgaggg caggttgtcg gagaaatcgg tatcgacggt ttccggtttg
     8461 ttttcggcag tttgggcgac agattccggt tcgggcgtgt cgatgacgat ttcgacaggg
     8521 ttgtacgggt tgaaggtctc gggctcgtac acgctgtctg tggattcgat ggcgttccaa
     8581 tcggcatccg cgcgtttttg ggtttcttca tcctgcgtaa gtgcgccgga taaaatgccg
     8641 ttttgcgcgg ctgccaggct gtcgaaatcc aagtcgatgc ggttggaagg cgtatcggtt
     8701 tcgacatcga acgtttgttt tgccgacaac tcttcttcag attccccatc taaggcaagt
     8761 gtgtcgttta catcgttttt cggagcgggt tcgggcgttg ccggagtttc gacttcggca
     8821 aaggtgattt ctatgccgtc gtctgccgcg tcgtcaaggt caggctcttc ctcagggacg
     8881 gattcttcgg tacggcgcgc gcgtttggat tgggcaaggc gcaaaagcag cagcagggcg
     8941 atcaatgccg cgcctccgcc ggcaagcagc aaggtgtacg aaccgccgaa cagtccgctt
     9001 tcggtttctt cttcggcaga aacctgttcg acaggttcgg aaacggcgtt accggtttcg
     9061 tcggtcggcg tgtcgatggc agaagcggcg gcttcttggg gggcggattc ggcagcggtt
     9121 tccgatgcgg cagtatttgc agcgggtaca ggttcgggtc gaacggccgg tttttccgct
     9181 tttgcttcgg gcgcggcaac ttttgcttca ggtttttcaa ccggtttctc taccgttgcc
     9241 tgtttggacg gttcggacgg catggatgcg gtttcggctt tgggtttcgc cgtttgcggt
     9301 ttgggttgtt ccgctttgat cctgttcaga ttcggaatgt gaagcacgct gcccgcacgc
     9361 agtctgccgt gtgcggaaac atttgggttt gccttcagca gcgcatcggc aacctgttcg
     9421 agcgtcaggt gtttcggacg gatggcggcg gcaatctgtt tgaccgtttc gcctttgcgg
     9481 acggtatggg ttttgccgtt gtacgccggt ttgacggctg cgttcgcgct gtctttttta
     9541 tcggttttgc ggagggcttt ggcgttttga ttttcttggg actctgctgt cggagcggtt
     9601 ttgcggtgtg tcttgccgtc tgaaagtgca gatttggttt tgggcgagta gccgacagga
     9661 tcgaggatgg cggtgtattc gcgtacctgt gcgcctgcgc cgatgcggaa taccaggacg
     9721 ggatcgcgga ctgcctgttc ggaagaaacg gcaatgacgg ctttgtcgcc caacttgtgg
     9781 actttggcgg tcaggccttt ttcggaaacg gtaacgctgc cgccgcctag cagggctttg
     9841 gcttcttcgc cggttacggt aatgctgccg gaaaagggtt cgtcaaggtt ggactggata
     9901 ttcagtccgc ccagtccagc atgtgcctga aaggatgcgg caactgcgac ggaggcggca
     9961 atcagtttga tttgtctgtt gtttttcaag atgtatcccc tgtgggttgg cggctgaata
    10021 cggtttgacc gcgtacagtc tgtaaatttc gtcatcatcg ggcatcggcg gggcagtcgg
    10081 ccggcgggca tttaatatgt gaatgtaccg accgccgcca cattttaaac ggcaatcatt
    10141 cgccgttttt acaaattatg acatatctcc atcttttttc aaaaacatct gtgcatattt
    10201 gcatcaatca aaacaaaatt tgttggtttt gcaggtgcaa aaacagggtt ctgcctgtat
    10261 gattagcgtt tatttgattt gctttctcat ttggatatga aattcgtcag cgaccttttg
    10321 tccgtcatcc tgtttttcgc cacctatacc gttaccaaaa acatgattgc cgcaacggcg
    10381 gtcgcattgg ttgccggtgt ggttcaggcg gcttttctgt attggaaata taaaaagctg
    10441 gatacgatgc agtgggtcgg attggtgctg attgtggtat tcggcggcgc aaccattgtt
    10501 ttgggcgaca gccgcttcat tatgtggaag ccgagcgttt tgttttggct gggcgcgctg
    10561 ttcctgtggg gcagccacct cgccggtaaa aacggcttga aggcgagtat cggcagggag
    10621 attcagcttt cagatgccgt atgggcgaaa ttgacgtata tgtgggtcgg ttttctgatt
    10681 tttatgggta ttgccaactg gtttgtgttt actaggtttg aagcgcaatg ggtcaactat
    10741 aaaatgttcg gctcgactgc actgatgctt gttttcttta ttattcaggg tatttatctg
    10801 agtacctgtc tgaaaaagga ggattgactg tggaatattt tatgttgctg gcaacagacg
    10861 gggagaatgt gtatgaagcg cgtatggcgg cacgtcccga acacctcaaa cggctggaga
    10921 cgctgaagtc ggaaggccgg ctgttgacgg caggcccgaa tcctttgccg gaggactcca
    10981 accgcgtttc gggcagtttg attgtggcgc agttcgagtc tttggatgcg gcagaggcgt
    11041 gggcggaaga cgatccctat gttcatgcag gcgtgtacag cgaagtgctg atcaagccgt
    11101 ttaaagcggt gttcaaataa tgccggccgt cgatttgatc cgcgaacgcc tgcagacgct
    11161 cgatccgctg gtgttggaaa tcggcgatga gagccatctg cacaaaggac acgcgggcaa
    11221 taccggcggc ggacattatg ccgttttggt cgtcagcggc cgttttgaag gcgtaagccg
    11281 cctgaaccgc cagaaaacgg tcaaatcgct gctcaaagat ttgttttcag gcggcatgat
    11341 tcacgcgctc ggcatccggg cggctacccc tgacgagtat ttccatacgg cggactgaat
    11401 gaagtctgcc cgaacatttc aatttaaaat ttaaagagag aagattatga aagcaaaaat
    11461 cctgacttcc gttgcactgc ttgcctgttc cggcagcctg tttgcccaaa cgctggcaac
    11521 cgtcaacggt cagaaaatcg acagttccgt catcgatgcg caggttgccg cattccgtgc
    11581 ggaaaacagc cgtgccgaag acacgccgca actgcgccaa tccctgctgg aaaacgaagt
    11641 ggtcaacacc gtggtcgcac aggaagtgaa acgtctgaaa ctcgaccggt cggcagagtt
    11701 taaaaatgcg cttgccaaat tgcgtgccga agcgaaaaag tcgggcgacg acaagaaacc
    11761 gtccttcaaa accgtttggc aggcggtaaa atatggcttg aacggcgagg catacgcgct
    11821 gcatatcgcc aaaacccaac cggtttccga gcaggaagta aaagccgcat atgacaatat
    11881 cagcggtttt tacaaaggta cgcaggaagt ccagttgggc gaaatcctga ccgacaagga
    11941 agaaaatgca aaaaaagcgg ttgccgactt gaaggcgaaa aaaggtttcg atgccgtctt
    12001 gaaacaatat tccctcaacg accgtaccaa acagaccggc gcgccggtcg gatatgtgcc
    12061 gctgaaagat ttggaacagg gtgttccgcc gctttatcag gcaattaagg acttgaaaaa
    12121 aggcgaattt acggcaacgc cgctgaaaaa cggcgatttc tacggcgttt attatgtcaa
    12181 cgacagccgc gaggtaaaag tgccttcttt tgatgaaatg aaaggacaga ttgccggcaa
    12241 ccttcaggcg gaacggattg accgtgccgt cggtgcactg ttgggcaagg caaacatcaa
    12301 acctgcaaaa taattctgaa aacgggatat ggcggcaaga cgttcagaca ggcgttttgc
    12361 cgccgcgcag gacagggaat accatgaaac agaaaaaaac cgctgccgca gttattgctg
    12421 caatgttggc aggttttgcg gcagccaaag cacccgaaat cgacccggct ttggtggata
    12481 cgctggtggc gcagatcatg cagcaggcag accggcatgc ggagcagtcc caaaaaccgg
    12541 acgggcaggc aatccgaaac gatgccgtcc gtcggctgca aactttggaa gttttgaaaa
    12601 acagggcatt gaaggaaggt ttggataagg ataaggatgt ccaaaaccgc tttaaaatcg
    12661 ccgaagcgtc tttttatgcc gaggagtacg tccgttttct ggaacgttcg gaaacggttt
    12721 ccgaaagcgc actgcgtcag ttttatgagc ggcaaatccg catgatcaaa ttgcagcagg
    12781 tcagcttcgc aaccgaagag gaggcgcgtc aggcgcagca gctcctgctc aaagggctgt
    12841 cttttgaagg gctgatgaag cgttatccga acgacgagca ggcttttgac ggtttcatta
    12901 tggcgcagca gcttcccgag ccgctggctt cgcagtttgc agcgatgaat cggggcgacg
    12961 ttacccgcga tccggtcaaa ttgggcgaac gctattatct gttcaaactc agcgaggtcg
    13021 ggaaaaaccc cgacgcgcag cctttcgagt tggtcagaaa ccagttggaa caaggtttga
    13081 gacaggaaaa agcccgcttg aaaatcgatg ccattttgga agaaaacggt gtcaaaccgt
    13141 aatggcattt ccaataccga tgccgtctga agccttttca gacggcattg cacgttcagg
    13201 taaggaggac ggcttatgcg tgcggtcata cagaaaacgg taggtgcaaa ggtggatgtc
    13261 gtgtccgaag ccggcacgga aacctgtggc aaaatcgacg gcgggtttgt cgtgttactc
    13321 ggcgtaacgc atagcgacac agaaaaagat gcgcactata tcgccgacaa aatcgcccat
    13381 ttgcgcgtgt ttgaagacga agcgggcaag ctgaacctgt ctttgaaaga tgtcggcggc
    13441 gcggtgctgc tggtgtcgca gtttacactt tatgccgacg cgacaagcgg gcggcggcct
    13501 tcgttttcgc aagccgcacc tgcagaacag gcgcagcagc tttacctgcg aacggcggaa
    13561 ctgttgcgcg gacacggaat tcatgtcgaa acagggcgtt tccgcacgca tatgcaggtg
    13621 tcgctctgca acgacgggcc ggtaaccata ctgctggact ctttcatgac gcggatttcc
    13681 ccaaaaatga aggttgttcc ggattgaaat tgaatccgca atggtgaaat atcgacaatg
    13741 aacgacaaca cgcaaaccct tcctccccgc cgcctgtccg tcgccccgat gttggactgg
    13801 acggacaggc actaccgtta tatggcgcgc cagattaccc gcaatacttg gctgtacagc
    13861 gaaatggtca acgccggcgc gattgtttac ggcgacaaag accgcttttt gatgtttaac
    13921 gaaggcgagc agcccgtcgc cctgcaactg ggcggcagcg acccgtccga tttggcgaaa
    13981 gccgccaaag ccgccgaaga atacggctac aacgaggtca acctcaactg cggctgcccc
    14041 agtccgcgcg tccagaaagg cgcgttcggc gcgtgtctga tgaacgaagt cgggctggtt
    14101 gccgactgcc tcaacgccat gcaggatgcg gtcaagattc ccgttaccgt caaacaccgc
    14161 atcggtgtgg acaggcagac cgaataccaa accgttgccg atttcgtcgg cacgctgcgc
    14221 gacaaaaccg cctgcaaaac ctttatcgtc cacgcacgta acgcgtggct ggacggtctt
    14281 tcacccaaag aaaaccgcga cgttccgccc ttgaaatacg attatgttta ccgcctcaag
    14341 caagagtttc ccgggctgga aatcatcatc aacggcggca tcacgaccaa tgaggaaatc
    14401 gcgcaacacc tgcaacacgt tgacggcgtg atggtcgggc gcgaggcgta ccacaacccg
    14461 atggtgatgc gcgaatggga caggctgttt tacggcgata cccgcagccc gattgaatac
    14521 gccgatttgg tgcagcgtct ctacacatac agtcaagccc aaatccaagc cggacgcggc
    14581 acaatcttgc gccacatcgt ccgccacagc ctcgggctga tgcacggtct gaaaaatgcc
    14641 cgcacttggc ggcgtatgct ttccgatgca acgctcttga aagacaacga cggcggcctg
    14701 attctcgaag cgtggaaaga agtcgaacgg gcaaatatgc gcgaataggg cggggctgtg
    14761 tgtatgaaat gccgtctgaa ggtttcagac ggcatttgtg cgtttgtcgg gcggtgttta
    14821 gggggcggta atggcgtgtt tcggcacttt gcccatatcc cagtgtgcca ccgcccagtc
    14881 gagcagttcg gcagggcggt cggcttccgg tgcttcgggc aggttgaggt aacggaacac
    14941 ttggcggagg agttgttcgc ggcggtttaa atccaatgcg ggggcgagcg tctgtttcga
    15001 ccatttctgc ccttgtgcgt tggtcagcag cggcaggtgg gcatattgcg gtgtcggaac
    15061 gtccaaacac tgctgcaaat agatttggcg cggcgtagaa acgagcaggt cttgtccgcg
    15121 gacgatgtgg gtaacgccct gttcggcatc gtcggcaacg acggcgagtt ggtatgccca
    15181 gtaaccgtct gcacgaagca ggacgaaatc gccgatgtcg ccggcgaggt tttgggcgta
    15241 accgccgacg atgccgtctg aaaagccgat gtcgcggtcg gggacgcgga tgcgccacgc
    15301 aggctgtttg ccttgcggtg cagggcgttg accggggtgg cggcagcgtc cgttatagac
    15361 gaacccgtct gcgccccgcc ttgccccggc ctgccagtct ttgcggctgc aatggcaggg
    15421 atagaccagt ccggcggttt tcaggcggca tagggtttct tcatacaggg cgtaacggcg
    15481 gctctgatag gcgacttctc cgtcccactc gaatccgaat gcctcaagcg tgtgcaggat
    15541 atggcttgcc gcccccggca tttcgcgcgg cggatcgagg tcttccatgc ggaccagcca
    15601 tttgccgccg tttgagcgtg catcggcata ggaagcgacg gcggtcagca gcgagccgat
    15661 gtggagcagc ccggtcgggc tgggggcaaa acgtcctgtg tacatatctg gtacagcccc
    15721 tttatttaag actattaatc aaagccatta tctcatcttt attcagttcc atcccgggtt
    15781 cttcaagtaa ggttaaatca tatagggcat tatattgctc ttcggtagct gaaccatcca
    15841 taaggccgga cgagaaaaaa tcgaaggctc tatctgcgat tctctcatta cttgcatttc
    15901 tactaaccag tttcatcaat tctgtatata ttgtatgggg aggtaccgga tccttaaaaa
    15961 tcagatctga ataaatttca ttgggtgaaa tgatttcgag tgcttttgct atggttctat
    16021 ttccttttgt gttggcgggt aatgtcgtgc attaacttct tgcccattaa tatttttagg
    16081 gcgaatcctt gatatgccgc actgtgtccg gtcaaaaggg cgatgccgtc tgaaagcctt
    16141 tcagacggca tcggggatga aaagtttaaa ccaacggtgc gagcagtttt tcaaacgctt
    16201 cttcaaactg tttcaaaccg tcttcctgca aacgcgttgc caaggtttcg acatcgatgc
    16261 cgagcgcggc ggtttcggcg agctgcgctt gtgcttcttc tacgccttcg gtcagcgtgg
    16321 ctttggctgt gccgtggtcg ataaaggctt tgagcgtggc attgggaacg gtgttgacgg
    16381 tgtgcgcgcc gatcaggctg tcaacgtaga gcgtgtcggg ataggctggg tttttcacgc
    16441 cggtagatgc ccataaaagc tgcacgcggt ttgcgccttg ggcttccagt gcggcaaatt
    16501 cgggggcggt gaaatattgt tcccaatctt gataggcggc tttggcaagg gcgatggcga
    16561 ttttgccttt gagccggtcg ggcagtgttg tgtccagcgc actgtccaca cgcgagatga
    16621 agaagctggc gacgacgtgg atatgggcaa cgctttgtcc ggctgccaag cgtttggcaa
    16681 tgccgcgcgc gtaggcggcg taggctttga gggtttgggc gcgtgagaac agcagggtca
    16741 ggttcacgct gatgccgtct gaaacgaggg tttcgagcgc atcgatgcct gcgtcggtgg
    16801 caggcacttt aatcatcgcg tttttgcacc cgatggcggc gtagaggcgg cgcgcttctt
    16861 caaccgtgcc ttgcgcgtct ttggacaatt cgggcgaaac ttcgaggctg acgaagccgg
    16921 ttttgccgcc ggtggattcg tgttcggcaa gacagacgcc gcaggcggca cgcacatcgg
    16981 caaccgccat cgtttcgtag cgttgtttgg gggtcaggtc ttgctgcttg agggcggcaa
    17041 cctcgtcggc ataaagcgcg tcgccggcga aggctttttg gaaaatggcg ggattggaag
    17101 ttacgccgca cacgccttgt ttcaacattt gcgccaattc gccgctttgc accagcgagc
    17161 gggaaaggtt gtccagccag atttgttgtc ctaatgcttt aacgtccgat aaaatagtca
    17221 tttctgattc ctttggatgg atggcggggt ttgaatgctt atgctacccc gattcggaaa
    17281 ttttgggtag ttttgttaca gcaaaggcgg atggcaatgg cagaaaacgg aaaatatctc
    17341 gactgggcgc gcgaagtgtt gcacaccgaa gcggaaggct tgcgcgaaat tgcagcggaa
    17401 ttggacgaaa acttcgccct tgcggcagac gcgttgttgc actgcaaggg cagggttgtt
    17461 atcacgggca tgggcaagtc gggacatatc gggcgcaaaa tggcggcaac catggcctcg
    17521 accggcacac ctgcgttttt tgtccaccct gcggaagcgg cacacggcga tttgggcatg
    17581 attgtggaca acgacgtggt cgtcgcgatt tccaattccg gtgaaagcga cgaaatcgcc
    17641 gccatcatcc ccgcgctcaa acgcaaagat atcacgcttg tctgcatcac cgcccgcccc
    17701 gattcaacca tggcgcgcca tgccgacatc cacatcacgg cgtcggtttc caaagaagcc
    17761 tgcccgctgg ggcttgcccc gaccaccagc accaccgccg ttatggcttt gggcgatgcg
    17821 ttggcggttg tcctgctgcg cgcccgcgcg ttcacgcccg acgacttcgc cttgagccac
    17881 cctgccggca gcctcggcaa acgcctactt ttgcgcgttg ccgacattat gcacaaaggc
    17941 ggcggcctgc ctgccgtccg actcggcacg cccttgaaag aagccatcgt cagcatgagt
    18001 gaaaaagggc tgggcatgtt ggcggtaacg gacgggcaag gccgtctgaa aggcgtattc
    18061 accgacggcg atttgcgccg cctgtttcaa gaatgcgaca attttaccgg tctttcgata
    18121 gacgaagtca tgcatacgca tcctaaaacc atctccgccg aacgtctcgc caccgaagcc
    18181 ctgaaagtca tgcaggcaaa ccatgtgaac gggcttctgg ttaccgatgc agatggcgtg
    18241 ctgatcggcg cgctgaatat gcacgacctt ttggcggcgc gg
//
LOCUS       DAWKOD010000025        17217 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000025 DAWKOD010000000
VERSION     DAWKOD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.025"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(109..948)
                     /locus_tag="WJK73_000648"
     CDS             complement(109..948)
                     /locus_tag="WJK73_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7557168.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1233..1308
                     /locus_tag="WJK73_000649"
     tRNA            1233..1308
                     /locus_tag="WJK73_000649"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Arg,seq:ccg)
     gene            complement(1373..2566)
                     /locus_tag="WJK73_000650"
     CDS             complement(1373..2566)
                     /locus_tag="WJK73_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7557169.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNRALVQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="WJK73_000651"
     CDS             complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="WJK73_000651"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ7557170.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3818..4918
                     /gene="aroC"
                     /locus_tag="WJK73_000652"
     CDS             3818..4918
                     /gene="aroC"
                     /locus_tag="WJK73_000652"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ7557171.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQIEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            5009..5434
                     /locus_tag="WJK73_000653"
     CDS             5009..5434
                     /locus_tag="WJK73_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ7557172.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAVAETPSVEAEAAESSAAE"
     gene            complement(5508..7493)
                     /gene="parE"
                     /locus_tag="WJK73_000654"
     CDS             complement(5508..7493)
                     /gene="parE"
                     /locus_tag="WJK73_000654"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ7557173.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7560..8084)
                     /locus_tag="WJK73_000655"
     CDS             complement(7560..8084)
                     /locus_tag="WJK73_000655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ7557174.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8249..9544
                     /gene="serS"
                     /locus_tag="WJK73_000656"
     CDS             8249..9544
                     /gene="serS"
                     /locus_tag="WJK73_000656"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ7557175.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSILDEGGLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9622..10620)
                     /locus_tag="WJK73_000657"
     CDS             complement(9622..10620)
                     /locus_tag="WJK73_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7557176.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10745..11821)
                     /gene="prfA"
                     /locus_tag="WJK73_000658"
     CDS             complement(10745..11821)
                     /gene="prfA"
                     /locus_tag="WJK73_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ7557177.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11925..12704)
                     /locus_tag="WJK73_000659"
     CDS             complement(11925..12704)
                     /locus_tag="WJK73_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7557178.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQLLEQTLKAHPTAWVGEIGL
                     DFYDKTQTPPQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGI
                     VHAFSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     KEINTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12819..13808)
                     /locus_tag="WJK73_000660"
     CDS             complement(12819..13808)
                     /locus_tag="WJK73_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ7557179.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13875..14558)
                     /locus_tag="WJK73_000661"
     CDS             complement(13875..14558)
                     /locus_tag="WJK73_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7557180.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14747..16081)
                     /gene="glmM"
                     /locus_tag="WJK73_000662"
     CDS             complement(14747..16081)
                     /gene="glmM"
                     /locus_tag="WJK73_000662"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ7557181.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16211..17062)
                     /gene="folP"
                     /locus_tag="WJK73_000663"
     CDS             complement(16211..17062)
                     /gene="folP"
                     /locus_tag="WJK73_000663"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ7557182.1"
                     /translation="MVGCVWQAGRFEIGLDKPKIMGIVNLSPDSLPDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVIMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPENMQINPKYQ
                     DVVGEVTQYLKARAAECVAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGELTGEADVAERVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
BASE COUNT         3823 a         4824 c         4594 g         3976 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aattcactat aaattcccat aaaaaaacgg
       61 agcagatacc tgccccgttt ttatttaatc cgaaatttta atctaaattt agaattttgc
      121 accggattgg tttgccatat agtcaacagc cgctttgact tcgtcatcgc tcaaacctgc
      181 attgccgcct ttggcaggca tcgcgttaaa gccttcaagg gcgtgtttgt gcaaggtttc
      241 tttgcctttt ttgatacgcg gtgcccaatc gtcttttttg cctatgccgg gaataccggg
      301 aatcgaaccg ccgtggcaca cctgacaggt tgcttcgaag acttttttac cgtcaacgcc
      361 gaccgcaggg gctgccgcac ccttgtcttc tgccttcgct tctgccggag ctgcactatc
      421 ggcaggagca gaagctgttc ctgaagcggc attgtcggca ggcgcagcct catcaggatt
      481 cgggaaagaa ccgccgcttt tgttcgccat gtaagtaatc gcccgtttaa gttcctgatc
      541 ggtcaggtct gccgcaccgc ctttggcagg catggcgtta aagccgttca gcgcgtgttg
      601 gaacaaggta tcgaagcctt gcgcgatacg cggtgcccaa tcgccgttgt gttccagttt
      661 cggagcgttc ggcacattgc tgtccgccgc gtggcattgg atacagattt tgccgaaaat
      721 ctgttcgcct tggcgttcgc cgacggggat gccgtcgccc atcgtcaatt gtccgacggg
      781 ctggatacgg gtctgcgttg ctgcttccgt agtggcatcg acatcgccga acgagccgct
      841 gcccgccagc ttaatcagga aataaaggac tgcaataaca ataacgatac cgctcacaag
      901 ggtaaacagt gcagagcctt gggctttgtt gtcgcggagt tgtttcattt ggtaggcctc
      961 gccgtcaggt taggttgtgc tgtaaattat agtttggtgt gttaaacgca gttaacaata
     1021 ttttgctgga ttatactgaa ttcacagggt ctttccaatc gctatcattg aaaatatgaa
     1081 aaaatttgcc aacggtatct gtataaaaca aacaatcctt tgaaaataat ggtttatcct
     1141 caagaaaact ctccttatgc cgccatacgc cgcctgccgg cgcaagataa cctttgccaa
     1201 tttgcagaat ttacgttaac cttacgtttt acgcacccat agctcagttg gaagagtgtc
     1261 agtttccgaa gctggaggtc acaggttcga tccctgttgg gtgcgccaat tataaagagg
     1321 ccgtctgaaa gataaatatt tttcagacgg tcttttgact tacttcaaac tcttatttca
     1381 agacttccgc aaatgcgcgg gcaacatagt cggtattcga cgtattcagt ccggcgacgc
     1441 acatcctgcc ggaatccagc aggtaaacgg caaattcgtc gcgcagcctg cggacttgtt
     1501 ccacgctcaa tcctgtgtag ccgaacatgc cgcgctgttt gatgaaataa gtgaaatcgc
     1561 gatcggggat ttgcgcagtt aagacatcat aaagtttctg ccgcatcgca cggatgcggt
     1621 cgcgcatcat ataaacctcg ttttgccaca aggcgtaaag ttcggggctg ttcatcacgt
     1681 cggcggcgat atacgcgccg tgcgcgggcg ggctggagta gatgcggcgg acggtgaatt
     1741 tgagctgtcc gaacaccaaa tccgcttctt ccttattcgg gcaaaccacg cttaagccgc
     1801 cgacgcgctc gccgtagagc gacaggtttt ttgagaagga attgctgacg aacaagggca
     1861 attccatttc caccgctttg cggacggcgt aggcatcgct gtccaaatcg ccgccgaagc
     1921 cttggtaggc aatgtccata aacggaatca gtttgcgcgt tttgatgatg tgcaacactt
     1981 cgtcccattg ttgttccgac atatccacgc cggtcgggtt gtggcagcag ggatggagga
     2041 tcaggacgct gttttcgggc agggtgttga aaaacgcggt catttcgtcg aatttcacgc
     2101 cgacagtggc agggtcgtaa tatgggtaag tgccgacctc gaaacctgcg ccttcaaaaa
     2161 tgccgcgatg gttgtcccaa gtcgggtcgc tgacgtaggc gcgcgcttcg ggaaaccagc
     2221 ggtgcaggaa gtccgccccg actttgagtg cgcccgagcc gcccaaggtt tgcacggtaa
     2281 cgatgcgtcc ttgcacaagc gcgcggttgt ctttgccgaa caataaatgc tgtaccgcgc
     2341 tgcggtaagt gtccaagcct tccatcggca ggtagggcga cggcgcaggc gcggcggcac
     2401 gggcggtttc ggcgcggctc acggattcca acacgggcat tttgccttcg tcgtcgaaat
     2461 aaatgcctat gctcaaattg actttttcgg ggcgcgggtc gtttttgaag gtttcgacca
     2521 aactcaaaat cgggtcgccg ggatagtatt cgatgtgtcg gtacatagtc cttacctttt
     2581 actttttcaa aggattttct ttttcaacaa tacaccactt tcgatatggt gcgtaaacgg
     2641 gaattggtcg aacagggcgg cacgttcgac cgcatgggtt tccgccaagg tgtccaaatt
     2701 ggcacgcagc gtttcgggat tgcaggaaat gtagatgatg ttgtcaaact gcgacaccag
     2761 cttcaaagtt tcctcatcga taccggcacg cggcggatcg acgaaaatag tggaaaatgc
     2821 gtaatccgtc aaagcaatac cgccatcctt aaggcgttta aactcacgtt ttccggtata
     2881 ggcttcggta aattcttcag cagacagacg ggcgattttg atgttgccga tgcggttggc
     2941 ttcgatattc cattgcgccg cgctgacgga ggttttggag atttcggttg ccaaaacacg
     3001 ttcaaacttt tcagataaag gaagcgtaaa gttgccgttt ccgcaataca gttcgagcaa
     3061 atccccatcc aatccttctg ctgcaccgca ggcccattca agcattttct gacacacggc
     3121 ggcattcggt tgggtaaaac tgccttcaat ttgccgataa cggaaatccc ggttgccgac
     3181 cttcaaagtt tccgttacat agtcctgttt taagactatt ttctgtcccc tgctccgccc
     3241 gatgacggaa atatccaact gttgctgtaa cgcttgcgcc gcctgcatcc actcattatc
     3301 aagccttttg tggtaaatca tggtaaccag catttccccg ctgagcgtag acaaaaattc
     3361 gacggcatac caacggtttc tcagttcggc agattgggcg gcaacggcaa tcaattctgg
     3421 catcaggcgg ttgacggctt cggaagccgc ttcaaaacgg tcgcaacgga tcaggcttgc
     3481 cccgccagcc ttttgtcctt tttcaaacat ggcataaaac atttcaccgc cttcgtgcca
     3541 aatacggaac tcggcacgca tacggtaatg tttgtccgga gattcgtaca cttcccactc
     3601 aggaacatcc aaacctgcaa aaagggtttt aaggtggtct tttttatctt gaagctgctg
     3661 cgtgtagtcg ctcatatcgc tatcctgaaa aatcagaccg gattataagc cgattcactg
     3721 caaaacacgc aaccccttta ccggcatata acccgttttc ctccaacttt tatgctaaaa
     3781 tccgccgtct ccaacatacc gccgacagaa ggaaaatatg gcaggcaaca ctttcggaca
     3841 actcttcacc gtgaccacgt tcggcgaaag ccacggcgca ggtttgggct gcatcatcga
     3901 cggctgcccg cccggactgg cattaaccga atcagacatc caagccgacc tcgaccggcg
     3961 caaacccggc accagccgcc acgttaccca acgccgcgaa gccgaccaaa tcgaaatcct
     4021 ctccggcgta ttcgaaggca aaaccaccgg cacgcccatc gccctcttaa tccgcaatac
     4081 cgaccagcgc agcaaagact acggcaacat cgcccaaagt ttccgccccg gccatgccga
     4141 ctatacctat tggcacaaat acggcacgcg cgactaccgc ggcggcggca ggagctccgc
     4201 ccgtgaaacc gccgcccgcg ttgctgccgg agccgttgcc aaaaaatggt tgaaagaaaa
     4261 attcggcacg gaaatcaccg cctacgttac ccaagtcggc gaaaaagaaa tccggtttga
     4321 aggctgcgaa cacatttccc aaaatccttt ttttgccgcc aaccatagcc aaattgccga
     4381 gctggaaaac tatatggaca gcgtgcgcaa atccttggat tccgtcggcg cgaagctgca
     4441 tattgaagcc gccaacgtcc ccgtcggctt gggcgaaccc gtattcgacc gcctcgatgc
     4501 cgaaatcgcc tacgccatga tgggcatcaa cgccgtcaaa ggcgtggaaa tcggcgcagg
     4561 ttttgacagc gtaacgcaac gcggcagcga gcatggcgac gagctcacgc ctcaaggctt
     4621 cctatccaac cactcaggcg gcatcttagg cggcatcagc accgggcaag acatctgcgt
     4681 caatatcgcc atcaaaccca ccagctccat cgccaccccg cgccgcagta tcgacatcaa
     4741 cggcaacccc atcgaactcg ccacgcacgg cagacacgat ccctgcgtcg gactgcgcgc
     4801 cgcgccgatt gcagaagcca tgctcgcgtt agtcctcatc gaccacgccc tgcgccatcg
     4861 cgcgcaaaat gcggatgttg ccgtggatac gcccgatatc gcccgctcgg acaagtaaaa
     4921 agatttagcc aaaacacggg ctttatacta aaataccgac cattgccgtg ccgtctgaac
     4981 gcacgattct tagaggaata caaccgaaat gacacaagaa accgctttgg gtgcggcact
     5041 gaaatccgcc gtccaaacta tgagcaaaaa gaaacagacc gaaatgatcg ccgaccacat
     5101 ctacggcaaa tacgatgtgt tcaaacgctt caaaccgttg gcactcggca tcgatcagga
     5161 tttgattgcc gctttgccgc aatacgattc cgcactgatt gcacgcgtcc tcgccaacca
     5221 ctgccgacgt ccgcgctatc tgaaagcctt ggcgcgcggc ggcaaacgtt tcgatttgaa
     5281 caaccgtttc aaaggcgaag ttacccccga agaacaggcg atcgcgcaaa accatccttt
     5341 cgtgcagcag gctttgcaac agcagtccgc ccaagctgtc gccgaaacgc cgtctgttga
     5401 agccgaagca gccgaatctt ccgcagcaga ataaatcccc aaacgaaatg ccgtctgaaa
     5461 accgatttgg tttcagacgg cattttttcg tatgcggcaa tcacggttca aatatcgagt
     5521 tccgccgtat cgccttcgcg ttccatccag gcacggcggg cggcggcttc gcctttgccc
     5581 atcagtttga cgaagatgtc gcgcgtctcg tcatccgcgc cttcggggat ttgcacctgc
     5641 aacaggcggc gggtgtcagg gtgcatggtg gtgtctttga gctggtcggg gttcatttcg
     5701 cccaagccct tgaaacggct gatggaatag gcggtttctt tgacgccttc tttttgcagc
     5761 cgctccaaaa tgctgtcgag ttcgttttgg tcgagggcgt agaatttgcg ggcaggtttg
     5821 cttttgcctt gtgcgttgac atcgacgcgg aacagcggcg gctgggcgac gtagatgtgt
     5881 ccgtcggcaa ccagtttcgg gaagtggcgg tagaataagg tcagcagcaa aacttgaata
     5941 tgcgagccgt ccacgtcggc atcggacagg atggcgattt tgccgtagcg caggccgctt
     6001 aaatcgggat ggtcgttaat accgtgcgga tcgacgccga tggcgacgga aatgtcgtgg
     6061 atttcggcgt tgccgaagag ttggtcgggg tggacttcaa agctgttgag cactttgccg
     6121 cgcaggggca ggatggcttg ggtggctttg tcgcgggcga gtttggcgga gccgccggcg
     6181 gaatcgcctt cgacaaggaa gagttcgttt tcgcggatgt cttcgctttc gcagtcggtc
     6241 agcttaccgg gcaggacggc gacgccgctg ccttttttct tttcaatctt tttaaccgaa
     6301 cgcatccgcg cctgtgcctg acggatagcg agttcggcga tttttttgcc gaagtccaca
     6361 ttttggttta gccacaattc caaaggatcg cccgatacag tggcgacaag tttcagcgcg
     6421 tcgcggttgg tcagcttgtc tttggtctga ccttggaact gcgggtcgag gacacgggca
     6481 gagaggacaa aggcggtttt gccgaacacg tcgtcgcttt gtactttcac gccgcgcggc
     6541 aagaggttgt gcagattgat gaaattgttg acggcgttga acacggcttg tttcaagcct
     6601 gcttcatgcg tgccgcccaa aggggtgggg atgaggttga catagctttc gttggcacac
     6661 gagccttctt ccagccaagt cagggcaaac gccgctcctt cgccgatgct gaaatcgccg
     6721 ttgtgttcgt ctgaaatgta gttttcgcaa gagaacagcg gcacagcttc ttgcgcgtcg
     6781 gcaatcaggt cggtcagata gcttttcagg ccgtcggggt agtgccaggt ttgggtgtgc
     6841 gcttcgtctt cgcctttgac cggacgggtc agggaaacgc gcacacccgg cagcagcacg
     6901 gctttggcac gcagcaggcg ttcgagttcg ggaatgctgt aattcgggct ttcaaaatat
     6961 ttgccgtccg gccagacgcg cacttcggtg ccgctgtctt tgacggcgca tttgcccact
     7021 tgtgccaacg gttcgaccac gtcgccgccg gcaaacacga tacggtggat tttgccttca
     7081 cgtttgaccg ttacctcaag gcgggtggag agggcgttgg tgacggatac gcccacgccg
     7141 tgcagtccgc ctgaaaaggc atacgcgctg ccgccgtctt ttttgttgaa cttgccgccc
     7201 gcgtgcagac gggtgaacac gagttcgact acgggtacgc cttcttcagg gtgcaggccg
     7261 acgggaatac cgcggccgtt atcacggacg gaaagcgaac cgtcttcatg aatttgcacg
     7321 tcgattgcag tcgcgaaacc acccaacgcc tcgtccgccg cattatcaat cacttcctga
     7381 cagatgtggg tcgggctgtc ggtgcgggtg tacatgccgg gacgttcttt gaccggctcc
     7441 aagcctttga ggacggtgat gctggattcg ctgtattggt tgtttttagc cataggaata
     7501 atctgaaagt aagaaaaaca acgctttcag acggcctgaa agcgttgcgt tccgttgttt
     7561 taccggttgc cggaaggttg gcgggcggca aagtcttcat aactttccat accgcgcagg
     7621 aagcgggaag agagttcttt caacgccccc aaataaacgt cgcgtttgaa ctcgatgact
     7681 tcgtcaacgg gcgcccaata ttgatgccaa cgccagccgt caaattcggg gtggcgggtg
     7741 gcgcgcaggt tgacatcgca atcccggccg gtcaggcgca ggagatacca aatctgcttc
     7801 tgcccgcgat aagagccgcg ccattcgcgg cgcacccagt tgttcggcac gtcgtaacgc
     7861 agccagtcgc gcgtccgccc gacgattttg acgtgttgcg gcagaagtcc gacttcttcg
     7921 taaagctcgc ggtacatcgc ggtttcgggg ctttcgcccg gcttgatgcc gccttgcgga
     7981 aactgccaag aatgttcgcg cacgcgctta ccccaaaaga cttcgtcgcg gttgttggtt
     8041 aagatgatac cgacattggg gcgatagcct tctctgtcca acacggtgtc gccctccgtt
     8101 aaattcaatc ttgggatttt cccacaaatc gaacggtttt gacaaatcag acggtatggt
     8161 tcaagcatgt cgaaacacag acggactcgg gaaaatatct taaatttagt ttacaataat
     8221 acgtttcaaa ttattcggga atcagaccat gttagatatc caattgctcc gcagcaacac
     8281 cgccgccgtt gccgaacggc ttgcacggcg cggttatgac tttgataccg cacgttttga
     8341 cgcactggaa gaacgacgca agtccgttca ggtgaaaact gaagaactac aagcctcgcg
     8401 caacagcatt tccaaacaaa tcggcgcact gaaaggtcag ggcaaacacg aagaagcgca
     8461 ggcggcgatg gatcaagtcg cccaaatcaa aaccgatttg gaacaggctg ccgccgattt
     8521 ggatgccgtt caaaaagaat tggacgcatg gttgttgagc atacctaacc tgccgcacga
     8581 aagcgtacct gccggtaaag acgaaaccga aaacgtcgaa gtccgcaaag tcggcacccc
     8641 gcgcgaattt gactttgaaa tcaaagacca tgtcgatttg ggcgaacctt tgggtttgga
     8701 tttcgaaggc ggcgcaaaac tctccggcgc acgatttacc gttatgcgcg gacaaatcgc
     8761 ccgtctgcac cgcgcgctgg cacaattcat gctcgatacg cacacactga aacacggcta
     8821 caccgagcat tacacgccct atattgtgga cgacaccacc ctgcaaggta cgggccagct
     8881 gccgaagttt gcggaagatt tgttccacgt tacccgtggc ggcgacgaga gcaaaaccac
     8941 gcaatatctg attccgacag ctgaagttac cctgaccaat accgttgcag gcagcatttt
     9001 agacgaaggc ggactgccgc tgaaactgac cgcccactcg ccctgcttcc gcagcgaggc
     9061 gggttcgtac ggcaaagaca cgcgcggtct gattcgccag caccagttcg acaaagtgga
     9121 aatggttcaa atcgttcatc ccgaaaaatc atacgaaacg ctggaagaaa tggtcggcca
     9181 tgccgaaaac atcctgaagg ctttggaact gccctaccgc gtgattaccc tgtgtaccgg
     9241 cgacatgggc ttcggcgcaa ccaaaacgta tgacttggaa gtttgggtac cggcgcaaaa
     9301 cacctaccgc gaaatctcaa gctgctccaa ctgcgaagat ttccaagccc gccgcatgaa
     9361 ggcgcgtttc aaagacgaaa acggcaaaaa ccgcttggta catactttga acggctccgg
     9421 cttggctgtc ggcagaacgc tggtcgccgt attggaaaac catcaaaacg ccgacggcag
     9481 catcaacatc cctgccgcac tgcaaccgta tatgggcggc gttgccaagt tggaagtgaa
     9541 ataagtttgc aagctgcctg aacgtcaaat gccgtctgaa acctggttca gacggcattt
     9601 cctttaaact tttaaaacac atcaaccgtc ggcacgaacc gcattgccgc aatcgccggt
     9661 ctgtccgacc tcgcggatat tggacagcgt aacttccgaa atattaccca acgcctcttc
     9721 cgtcaaaaat gcctgatggc cggtaaacag cacattatga caagacgaca ggcggcggaa
     9781 cacgtcgtcg gtaatcacat cgttggattt gtcttcaaaa aacaattcgc gttcgttctc
     9841 gtacacgtcc atacccaatg cgccgatttt ccggcgtttc aacgcctcaa tcgcggcaga
     9901 actgtcaatc agaccgccac ggctggtgtt gataatcatc acgccgtctt tcattttgtc
     9961 gaacgccgct tcgttcagca tatagtggtt ttccggcgtg gaggggcaat gcagcgtgat
    10021 gatgtccgac cgggcataca gctcgttcaa ctccacatac ctgccgccga ttttttccgc
    10081 ttcggggttg caaaacggat cgtaagccag caggttcatg ccgaaaccct ttaaaatccg
    10141 catggttgca ataccgattt tccccgtgcc gataacgccc gccgttttgc cgtacatatt
    10201 gaaaccggtc agaccttcca gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg
    10261 gatacggcgg ttcaacgtca gcatcagacc gaccgtatgt tccgcaaccg attcgggcga
    10321 ataggcaggc acgcgcacga ctttcaagcc caactcttca gccgccttta aatccacatt
    10381 attgaagccg gcacaacgca acgccacagt tttcacgcca atttgcgcca atttttccaa
    10441 cacgggccgg ctgccgtcgt cgttcacaaa aatacagacc gcctccgcac cttccgccat
    10501 tttcgccgtt ttcgcatcca gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg
    10561 gttggcgcgg gtaaaatgtt cgcggtcata gcttttcgta ccgtaaatcg caatcttcat
    10621 caatatgtcc agttgtcgtc tatggttgag aaacggcata atacactgaa ttcaaacaaa
    10681 tcagtagaat atggtggatt aaaattgatt gcatgcacgg catttccatt tcaaaacaca
    10741 aaactcaatc gcccattgcc gccagaagct ccgcctgatg ctcggcaatc agggcatcgg
    10801 tgatttctgc caaatcgccg tccatcacaa aatccagctt gtgcagggtg aggttgatgc
    10861 ggtggtctgt taccctgcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtctc
    10921 cgctgccgat gagggatttg cgttcggcgg cttctttggc ttgggcttcg cgtttttgcg
    10981 cgtcgttcag gcgggcggcg aggactttca tcgcctgcgc tttgttggcg tgttggctgc
    11041 gtccgtcttg gcattcgacc accatgcctg tgggcaggtg ggtgatgcgg acggcggaat
    11101 cggttttatt gatgtgctgc ccgcccgcgc cggatgcgcg aaaggtgtcg atgcgaaggt
    11161 cgacggggtt caactcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc
    11221 aggcggaggt gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc
    11281 cgcccgattc aaatttcaaa cggctgtacg cgcctaagcc gataatacgg gcgataactt
    11341 ctttatagcc gcccaactcg ctttcgttgg cggacacgat ttcaacctgc cagcggttgc
    11401 gttcggcgta gcggctgtac atacgcagca aatcgccagc aaacagcgcg gcttcgtcgc
    11461 cgcccgttcc ggcgcgtact tcgataaaga tgtttttgtc gtcgtcggca tctttgggca
    11521 gcagcagttt ttgcaattcg gtatcgagtg cgccgatttt ggctttcgcc gcttcgattt
    11581 cttcggcggc aaagtctttc atttcggggt cggacaacat ttcttcggca tccgccaagt
    11641 cgctttgggc aagccgatag ttttggaaca cttcgacgac gggggtcagt tcagcgtgtt
    11701 cgcgcgtgag cttgcggtag ttgtccatat cggacgtggc ttcgggttgc cctaaaaggt
    11761 gggtaacttc ttccaaccgg tcgccgagtt gttgcagttt ttctaagata gacggcttca
    11821 taattcttcc ataacaaacg ccgcctgaat ttcagacggc atcaacactg aattattata
    11881 ataggttttc cggacattca aaaagataat ctcagatgga taacctaccg tcccaacagg
    11941 gcatcggcat tgcgctccgt taccttggca atctcttcca cacaggttcc gcggatttcc
    12001 gcagcaatct ttgcaatacc cggaatattg gcaggcgtat taatctcttt tttcagcata
    12061 aacgggctgt cggtttccaa tacgaaatcc ccgtcgttca aggctttaag cgtatcgcgc
    12121 actttacgcg cgttcggatt gagcaacaac gaaccgatgc cgattttgaa acccagtttc
    12181 gtcaacacgc gcgcttcttc cgcgctgcct gagaaggcgt gaacgatgcc gccttgggca
    12241 aagcctgtct gtttgacggc ggcggcgatg tcggcggtgg ctttcagatt atggataatc
    12301 acgcggcggc gcagggtttg cgcgatttca agctggcgga cgaaaacttg gatttgccgt
    12361 tcgcgctgcg gcggcgtttg ggttttgtcg taaaaatcca agccgatttc gccgacccat
    12421 gctgtcggat gcgctttcaa tgtttgttcc aaaagctgga aatccggttc ggaaacgtcg
    12481 tctgaaaacc aaggatggat gccgagcgcg atatggatat tgccccaaag cggattttca
    12541 gacaaccttt gagccaaatc cgccacgtcc tgccaatcct gcggccgagt cgcgggaacg
    12601 ataaaccgcc ctacccctgc ctcccgcgct gcggtcagga tgtgcggcag gttttcgcgc
    12661 agggcggcat cggcaaggtg gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc
    12721 ggcaggttca cataaaaata aggcagggcg gcaagggatg cagtgtacgg gcggcgcata
    12781 aggcgcggtc aacgccccgt cattgccatt tcaatccgct ataacttcag tcttgccaac
    12841 tctttttcca cctcttcctt accccagcct tgcgatacgg cgagggtcat aagcgcggtg
    12901 gcggtttcga ggttgcattt gccgccgttg atgatgcctg agttgcggaa cgcgttgcct
    12961 tgcgcgtaaa cggcagcggt tctgccttgt cggacttggc tgatgttgag cagcagtttg
    13021 ccttgctgcg taaagtcttg cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg
    13081 tgtccgtaac tttgcaggat aagggcgtgg gcgggaagct gtccgagtcc gtcggcaagt
    13141 tcttggacgg caaagccggg gataagcgtg cggacggcga tttttgcctg cgggtcggga
    13201 taacggattt tgaggccgtc tgaaacatct gccgcgtcct gattcgggat gcggacattg
    13261 tgccagccct gggtttcgtc ccactccgcc agtgcgccga aatgcggatt gtcgaaaccg
    13321 gcggcggttt cggtgctgac tttgctgctg ccgacggcgg gataaagttt gccgtcaaag
    13381 gcaataagcg tctgcttcaa tccgagtggg aatgcggcaa cggcagtcgc cagattgcgc
    13441 ggcgcgtcgc tgcccacggc gttgtacggc cattgggagc cggtcaggac gattggcttg
    13501 tccaaaccct gcagggcgag ggcgaacaga ttggcggtat aggcgagcgt gtccgtgccg
    13561 tgcagcacca aaacgccgtc gtactgcggc agcgcggcgg caatgatgtc cagccaagcg
    13621 caccagtttt gcagttggac ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg
    13681 aaatccatgc cgtttgaaaa cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc
    13741 aggccttcgc tgctttgcgt catgccgatg gtgccgccgg tataaaggac aaaaattttc
    13801 tgtttcatgg ctcatcctga aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg
    13861 gtcgaaaatc tgatttactg ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg
    13921 cgtttggcgc gtttttcgct caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc
    13981 caccagaacc acgccagcgc cgccgccagc acgcccaatg cgatgaagat gccggattgc
    14041 aggctgtgca ttttcgccat cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa
    14101 atccaaacgg gcacggaaat cagcgcggca agcccgtcca taatcagaaa gcgcagatac
    14161 gatactttgc ggctgatgcc ggcggtaacg aaaacggcag tccgcaaacc cggcaggaag
    14221 cgggcgacaa acagaaccca gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa
    14281 cgtttcggcg tcatgatgcg cgcaataggt ttgaacttga ggattttctg cccccagatg
    14341 cgtccggcgg cgaacatgat gccgtccccg accaatacgc cgagcatacc gactgcaaac
    14401 ataatatgcg gattggtata acccataccc gaaatcacgc cgcctgttac caaggtcaaa
    14461 tcctcgggaa tcggcacgcc gaaaccgcag atgaccaaaa cgaaaaacac ggccgcatag
    14521 ccgtattcga caaaaaaggc ttccaaaagg gcaaacatag cggatattcc attgtcggag
    14581 ataaaaagtc agaacaagcc gaaacatttt ctgcgcgaaa cggcattcta tcaaagatta
    14641 tgccgtctga aagcggcaag gggcagattc gcgccctctg ccggacgttc agacggcatt
    14701 taaacaggaa aaccgccgct gttgaaacgg ttttgcctga ttttgcctaa aggctgccga
    14761 tggcggaagc aatacgttcc gcaccgtcgc gcgcccaatc cgcctgccgc gcctccacca
    14821 tcacacgcac gacgggttcg gttcccgaag cgcgcaacac gacacgccct ttgccttcga
    14881 gttctttttc cacttccgcc aacacgtctt ttgaggcttc ctgccatttc tgaccttttt
    14941 ggatgcgcac gttaatcata gtttgcggat acggctgcca atcggcgcaa acggtggcga
    15001 ggtcttggtt cagcgtttgc agtgccgcca aaacttgcag cgcggaaata atgccgtcgc
    15061 cggtgttgtg tttgtccatg cacaaaatat ggccgctggc ttcgccgccg atgagccagc
    15121 cgcgttggtt cagctgttcc aacacatagc ggtcgccgac tttggcgcgg cagaaatcca
    15181 cgccctgctc tttcagggcg atttccatcg ccatattggt catgaccgtg ccgaccacgc
    15241 cgccgatgtt gataccttct cgggcgcggg ctttagcaat gacgtaaatc aggctgtcgc
    15301 cgtcgtaaac cttaccgttt ttatcgacca tcatcaggcg gtctccgtcg ccgtctaagg
    15361 cgatgccgta gtcggcttca tgctgtaaaa cggctgcctg gagtgtcttg gtataagtcg
    15421 caccgcattt ttcattgata ttgtagccgt tgggttcgtc gccgatgctg acgacttgtg
    15481 cgcccagttc gtgaaacacc ttgggtgcga caccgtaccc cgcgccgttg gcagtatcga
    15541 taaccaactt caaaccccga aggtcggaat ggctgggaaa ggtagatttg caaaattcga
    15601 tatagcggtc gtccgcacca ctgatgcggc gtgcgcgacc gaggcgtgca gaaggctggg
    15661 ttttcatttc ttcgtcgatt ttggcttcga tttccaactc gatttcatcg ctcagtttca
    15721 cgccgccctc ggcaaagaat ttgatgccgt tgtcggaata ggcgttgtgc gacgcggaaa
    15781 tcatcacgcc ggcggacagg cgcaacgcgc gggtcagata agccacgccg ggcgtgggca
    15841 gcggtccggt ctgtaccaca ttcacacccg ccgccgtaaa accggccacc aaagcggctt
    15901 ccagcatata gccggaaatg cgcgtgtctt tgccgatgag gacggtcggt ttctggtcgg
    15961 tgtcgtgctg caccaaaacc tgccccgccg catagccgag tttcaatacg aaatcgggcg
    16021 taatcggaaa ctgcccgact tcgccgcgca cgccgtccgt gccgaaatat ttttttgcca
    16081 tatgttgctc cgagaatgtg aaccgttgtc cgagattata cagtctgttt gtgccctgct
    16141 gtctgcaccg ttgatgccgt ctgaagccgc cccgtccttt tcagacggca taaagtatgc
    16201 gaaccgctgt ttacagattg atgcccaacg cttcccacac cttcaacgca tccgccgtcg
    16261 ccttcacatc atgcacccgc acgatttgcg cgccgcgcgc cacggaagcc agcgccgccg
    16321 ccacgctgcc gtgtacgcgt tccgccacgt ctgcctcgcc ggtcagctcg cctatcatgc
    16381 ttttgcgcga tacgccgata agcagcggca aaccggtttc cgccatcaat tcgggcaaat
    16441 gccgcatcag cgcgatattg tgttgcaggg ttttgccgaa gccgaaaccc ggatcgagcg
    16501 tgatgcgttg cggtgcgata cctgccgcga cacattccgc cgcccgcgct ttcaaatact
    16561 gcgtcacttc accgacaaca tcctgatatt tcggattaat ctgcatgttt tcaggcaaac
    16621 cctgcatgtg catcaggcaa atacccgtgt ccgtctggcg cgccagcaat tcgaccgcgc
    16681 cttcgtcggt caacgccgcc acatcgttaa taatatcgat gccgccgagt gccaatgctt
    16741 tttccataat caccgtgcgg cgcgtgtcca aactgacggg aacgccccac cccgccactt
    16801 ctgccaatac aggttcaacc cgcgcccatt cttcttcagg cgaaacataa tccgcgcccg
    16861 gccgcgtcga ttcaccgccg atgtcgagaa tgtccgcacc ctctttcaaa agccgttcgg
    16921 catgcgccaa agctgtttgg gcattttgcg aatacgcgcc gccgtcggga agagaatcgg
    16981 gcgagagatt cacgatgccc atgattttcg gcttgtccaa accgatttca aaccgtcctg
    17041 cctgccaaac gcatccgacc atatccgtgc ctccggaata aaaagcaaat tatatgccgt
    17101 ccgaacaaaa cttgtccatt tcagacggca tcggcatttc aaaaaagcct cccgccaact
    17161 ccgccggcgt atgaaatatg cccgcccctg cgaacgcggc aacgcccctt tatgcaa
//
LOCUS       DAWKOD010000026        17058 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000026 DAWKOD010000000
VERSION     DAWKOD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.026"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(16..771)
                     /locus_tag="WJK73_000664"
     CDS             complement(16..771)
                     /locus_tag="WJK73_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ7557183.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WJK73_000665"
     CDS             complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WJK73_000665"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ7557184.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2481..4736
                     /locus_tag="WJK73_000666"
     CDS             2481..4736
                     /locus_tag="WJK73_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ7557185.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5000..5704)
                     /locus_tag="WJK73_000667"
     CDS             complement(5000..5704)
                     /locus_tag="WJK73_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557186.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            5921..6184
                     /locus_tag="WJK73_000668"
     CDS             5921..6184
                     /locus_tag="WJK73_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7557187.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6262..8307
                     /locus_tag="WJK73_000669"
     CDS             6262..8307
                     /locus_tag="WJK73_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7557188.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDVWVGDGHSFKAKIRHPMGYLFTPEVTMIVDGCSGAVVGW
                     SVALSETAVAVADALRHGMTHFPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFT
                     GIPGNPQGRGKIERLWQTITIPLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGK
                     ELSKAQEAALSAAPTWAQFIADLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYR
                     METDGMRVKTDVLSPLELDFMYRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVA
                     YDIHDADHVIVKDMQGKVICKAVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDL
                     IEAQRQSAAPIIEQQPDFGEFLKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8349..9263
                     /locus_tag="WJK73_000670"
     CDS             8349..9263
                     /locus_tag="WJK73_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7557189.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9339..9581
                     /locus_tag="WJK73_000671"
     CDS             9339..9581
                     /locus_tag="WJK73_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557190.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9571..9870
                     /locus_tag="WJK73_000672"
     CDS             9571..9870
                     /locus_tag="WJK73_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557191.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            9873..10025
                     /locus_tag="WJK73_000673"
     CDS             9873..10025
                     /locus_tag="WJK73_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557192.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10041..10205
                     /locus_tag="WJK73_000674"
     CDS             10041..10205
                     /locus_tag="WJK73_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557193.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10186..10401
                     /locus_tag="WJK73_000675"
     CDS             10186..10401
                     /locus_tag="WJK73_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557194.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10398..10712
                     /locus_tag="WJK73_000676"
     CDS             10398..10712
                     /locus_tag="WJK73_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557195.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            10831..11208
                     /locus_tag="WJK73_000677"
     CDS             10831..11208
                     /locus_tag="WJK73_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557196.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11192..11647
                     /locus_tag="WJK73_000678"
     CDS             11192..11647
                     /locus_tag="WJK73_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557197.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11619..11813
                     /locus_tag="WJK73_000679"
     CDS             11619..11813
                     /locus_tag="WJK73_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557198.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            11806..12417
                     /locus_tag="WJK73_000680"
     CDS             11806..12417
                     /locus_tag="WJK73_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ7557199.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12423..13073
                     /locus_tag="WJK73_000681"
     CDS             12423..13073
                     /locus_tag="WJK73_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557200.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13074..13274
                     /locus_tag="WJK73_000682"
     CDS             13074..13274
                     /locus_tag="WJK73_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557201.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13290..13598
                     /locus_tag="WJK73_000683"
     CDS             13290..13598
                     /locus_tag="WJK73_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7557202.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNV
                     "
     gene            13666..14100
                     /locus_tag="WJK73_000684"
     CDS             13666..14100
                     /locus_tag="WJK73_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ7557203.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14097..14543
                     /locus_tag="WJK73_000685"
     CDS             14097..14543
                     /locus_tag="WJK73_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557204.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14591..15232
                     /locus_tag="WJK73_000686"
     CDS             14591..15232
                     /locus_tag="WJK73_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557205.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15364..15909
                     /locus_tag="WJK73_000687"
     CDS             15364..15909
                     /locus_tag="WJK73_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7557206.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            15906..16112
                     /locus_tag="WJK73_000688"
     CDS             15906..16112
                     /locus_tag="WJK73_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557207.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            16115..16279
                     /locus_tag="WJK73_000689"
     CDS             16115..16279
                     /locus_tag="WJK73_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557208.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            16282..16518
                     /locus_tag="WJK73_000690"
     CDS             16282..16518
                     /locus_tag="WJK73_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557209.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            16490..16909
                     /locus_tag="WJK73_000691"
     CDS             16490..16909
                     /locus_tag="WJK73_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557210.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
BASE COUNT         4411 a         3793 c         4903 g         3951 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgcgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccaata gacgcgccgc tgcaaaacat ttgcgcattg gcatttccgg aggaaagcag
      181 gctgctgttg aaatcttccg tccattccca aataagtccg tgcatatcat aaacgcccca
      241 gtagttggga cggcccttgc cgacgtcatg caagcctttt cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gtaaacttca tatcatgcct
      781 cctgacaggc agttaaaatt gatttttcag acggcatttt aaacaaaaag ccgcgaatgc
      841 cgtctgaaga atcaatcttc cgaaaggaaa gattggttgt taaaaaacca ctgccgtgcg
      901 taattaagta cggcggcagt ggttcgtttg cctaaaaatg ggtgttcaat ttagtaatcg
      961 ctttttgcag gagcagaggc tgcaggagca gaagctgcgg gagcggaagc agcaggagct
     1021 gcaccgttac cggcgtaagc ggtatcactc aatttttgag tcatgatttc agggttttct
     1081 gcaccctcta ctttcaattg acccagtgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcaaccaaag tgtagctgcc cgggatgtcg actttgaatt cgacgatggc agagccgccg
     1201 gcaggaacga tggtgctttg tacgttttcg ttaatcagtt tgccgccttc aacataaact
     1261 ttgtcgaaga tttcaccgat tacgtggaag gaagacacca agttaggacc accgttacca
     1321 acgtacatac gtacagtttc gcctgctttg gctttcaggg cgttgtcgcc ggcgatagca
     1381 cctacgtgac cgttaaatac aacgtattca ggttgttcgg cgatggcttt gtccatatcg
     1441 aacggttgca ggccttgcgc gccttttttg cctttggtgt agaagtcgcc ttggacgatg
     1501 tagaactctt tatccacttt cggcagacct tctttaggct cgaccaaaat cagaccgtac
     1561 ataccgttgg cgatgtgcat accgaccggc gcgacggcgc agtggtagat gtacagaccc
     1621 ggttgcaggg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcggcc
     1681 gcgccgccac cttggccggt agctgcatgg aagtcaacgt tatggggaac ggtagaagaa
     1741 ggattgttag agaattctac ttctacggta tcaccttcgc gtacgcgaat catacggccc
     1801 ggaacgtcgc cgtcaaatgt ccagtagcgg tattccaccc cgtcgtccat tttcatggtt
     1861 ttttcgacgg tttccatttt tacgcgtact ttggcaggat agtcgcggtc gattgcggga
     1921 ggtacttcag gagcgtgggt ggtaactgca tcgatgacgg gcagttcgcc tgcaggcgtt
     1981 tcggcggcgg tttgtgcggc ggagcttgcg gcttcggcgg aagcggcagg ggtttgcgcg
     2041 gcaggttcgc caccgcaggc ggctaaagca aacatagaag cgataattgc ggctaaggct
     2101 tggcgtttca taatgttttc cttttgtaag aaaagtaggg agattggtta agaactggtg
     2161 tgattatgcg tttgccccat caccgacatt ttgatttaag tcaaataatt cataaaatat
     2221 gaacttaaaa tgatacttct aatactttat agctactaaa aattacaata aataggctga
     2281 tttgcttaag ggcttcggca aatcaggcaa aggcagggta gttgccgact aaagaataat
     2341 gatagttatt atcatatatg tatttgtttt atatgtgagt ttctttgaac aaatgtttat
     2401 ttgcgcggta aaccgtgcta caatctttaa cattcatatt ttgtgaattt taatccacta
     2461 tattaaaagg agctctcaaa atgggacagt acaagaagct gtggtacttg ctgtttgccg
     2521 ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat aagaaagccc
     2581 cgccttaccc cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata
     2641 ttttggcagg tcagtctgcg tggcagagta ccggcggtat ggaagtcggt tcgattctgg
     2701 gtcacggcgc atatcaggct ccggactgga cggcggactg gctgcatcgt gagttgtccg
     2761 cttggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa gtttcccctg
     2821 aagaacaagc cgttctgaaa acccgactgg ctgacgaata ccgcaaccaa agccgtatta
     2881 aagaagacgg cagcgtcgtc atcagcgata cgcgcgcgaa agccatcgaa agcatcctgc
     2941 cttattacca cggtgtttac ggcgacgatc ccgcgctgca aaccacccgc gaacactttg
     3001 caatgaaaaa caacacattg ccaagtcagg aagcgcgtga aaaactgttc gacttcttct
     3061 tctggacttc ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca
     3121 actggccgca cgagcctttg attaacaatg taccgactac tgaaaactac atgtggtcgt
     3181 ttaccagtgt tgtactgctc ttgatgggca tcggtttgtt gatgtggggt tattccttcc
     3241 tgaccaaaca cgaggaagtg gaagtgccgt ctgaagaccc gatttccaaa atccaactga
     3301 ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggttgccctg tttgtggtac
     3361 aagtattgct gggcggtctg actgcgcact acaccgttga aggtcagggt ttctacggca
     3421 ttgacgaggc gttgggcttt gaaatgtccg actggttccc ctacgccctg acccgtactt
     3481 ggcacatcca atccgccatc ttctggattg caaccggctt tttgacggcg ggtttgttcc
     3541 tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtaaacttcc
     3601 tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga
     3661 cgcacatcct tccgccggaa tttaacttct ggttcggaca ccaaggttac gaatacctcg
     3721 atttgggacg tttctggcaa ctcctgctga tggtcggcct gctgttgtgg ctgttcctga
     3781 tgttgcgctg cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa
     3841 tctttgtcgc ttctatggtc ggtgtgggcg tattctacgc accaggcctg ttctatggcg
     3901 aaaaatcccc gattgccgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg
     3961 aaggcttctt tgaagtattt gccactgccg cttttgcctt tgtcttctac aatatgggct
     4021 ttgtccgccg cagtaccgcc acggcctcta ctctggccgc tgccgccatc tttatgttgg
     4081 gcggcgttcc gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg
     4141 caatcggtgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg ggccgtgaag
     4201 catacgagca ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc
     4261 tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggtgta ttcggtttcc
     4321 tgattaaccc gccgatttcc ctgttctata tccaaggttt gaatacttct gctgttcatg
     4381 cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg
     4441 cccgttattt gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggc
     4501 tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gttgccggtt ggcgtgattc
     4561 aggcatacgc ctccatcacg cacggtctgt ggtatgcccg cagcgaagag ttcctgcaaa
     4621 tggaaatcct cgataccttg cgttgggtac gtacggcagc tgacttggtc tttatcggcg
     4681 gtgcaatctg cgttgccatc caagctacta aaatcgtatt cggccgcgac aaataagttt
     4741 tgcggtatga aatgagcaac ttatggggta ggtattattt tgacaccttt agaaagaaag
     4801 caaaagaagg cgaaaatttt aaaggaaaca aaaatttgaa cggaaattta ggaaaaagcg
     4861 gctttgacac ctttaggtgc aaagtaggta gaaagtgata taactttgat accttttttg
     4921 cacggttttg atgaggtttt gggttgattt tggttttatt tgaattgaac ggcgtttgga
     4981 atatccaaac gccgtttttt tagttgacgg tacggccata ccattctacg cgtccgatga
     5041 tggctacatt ttggttgtcg tcggttaaat ctatttcgaa aggctcataa accggattgg
     5101 ctgatttgac gagcagcttg ccgggtagtt tttggatttg tttgacaaac aggtgattat
     5161 cgatgcggat aacgtacagg ccgtcgcgcg gggtggtttc ggcgtgattc actaggatgt
     5221 tgtcgccgtg gttgagtacg ccctccatcg aatcgccctt tacggcgatg acggacagtt
     5281 tgtcaagctg gcgggtaacg tagttttcta tccaataacg cctgaatgcc atgcagaata
     5341 agggcttgtc gtcttctgcg ggatagccgt gccctgcggc ggcgtagacg tcgtaacgcg
     5401 ggataaagac gaactcgtct aagttgatgg ggttgccgag ggtatcggta gccgtaccgt
     5461 cggtatgggt acggacttcg accgcttggc ctttattcat gtatggcgag ccttggccgg
     5521 taagaagcca gtttaaatcg caaccggtta cttcttgaat tttaatcaga tagtctgctg
     5581 ttggaatggc accgtctttc cagacacggc taaaaccgga ggcagacata tcaatcttat
     5641 tgtagaagtc ggcgggtttg gcattgtccg gccacaaaga tttcaaacgc tctaaaaagt
     5701 ccatgattgt ccttttgctt agaatttcgc atttaagcaa aaaatacact tcttttgctt
     5761 aaaattaatt cgtcaaataa tcaaatagat ggctaatatt ttgctttttt gcgttatatt
     5821 tctaagcaaa agagttgcaa taaccttttt tgcttagtat tattcactca cttaatcaat
     5881 taacgaagta aatcacatta ctgaatggaa taagtcaatt atgcaaaaaa acgcaacgcc
     5941 gaaaaactgg caccgcgcgg atattgttgc tgctttgaaa aagaaaggct ggtcgcttcg
     6001 agcactttca atagaagcgg ggttgtcgcc gaatacgctt agaagcgcac tggccgcccc
     6061 ttatcttaag ggagaaagga ttattgccgc tgcaatcgga gtggaaccgg aagagatttg
     6121 gcccgaacgg tatgcagatc ggaatttaaa acctgttttc cccaaaagag tagttaatgg
     6181 ataagtggtt ttcgatttag ttccttaaat gctaaaggtt atacgcattt aagtaaaaca
     6241 caaaaagaaa gaaatagcaa aatgttgatt tcagcggttg agttggcggg cttgcaactt
     6301 ccaaagctgc cgaacagtag acaaggcatc gagtaccacg ccaaaaagaa taattggccg
     6361 tttgaagagg ttgtaggaca ggctcgaggc ggcaagctta agaaatattt ggtttccgcc
     6421 ctcccttcgg agattcaggc ggccatacag gaaaaacagg cggcaacatt gttggccaag
     6481 gtgccgatgt tgccggcaga agtaaaaaaa ccgcttcggc aaaataaaaa gatgcggcag
     6541 ttgggtttga taccgtgcga ggaaggtttg gcgcggttgg acgacaggca gacggaaacg
     6601 gcccacgcgc gttgtgcgat tgtcgcctat gtattgccgc tgcatgaatt ggcgggtatg
     6661 ccgattaaga aggcggtggc gtttgtggca gctgaagcgg cagcaggcag gttgccggag
     6721 gatgtggcca agctgattcc gttggcgaat gcgcggaaca acggcgaacg cagtttaagc
     6781 gaaccgactt tataccgatg ggtgcgggct taccgtgcgg catcggacag tatgagccgc
     6841 ctcttggcac ttgcgccggt gaaaacacgg gaaaagacgc cgctgctggc gattgactgg
     6901 ctgccttact tcttgatgtt ttatcagcgg ccgaacaagc cgacgatgat ggcggcggcg
     6961 aagaaactgg cgcaatggta tctggaacag ggaaaaatcg agcagatgcc gagctatgac
     7021 cagattcaaa cagtgatgaa acggttgccg gaacatatga aggagcgcgg caggcgcacg
     7081 gggtcggctt ataaggcttt gctgccttac attgatagag attggacggc gttgaaaccg
     7141 aacgatgtct gggtgggcga cggccacagc tttaaagcaa aaatccggca tccgatgggc
     7201 tatctgttta cgccggaagt gacaatgatt gtggacggtt gcagcggtgc ggtcgtgggt
     7261 tggagtgtgg cgctgtcgga aacggcggta gcggtggcgg atgctttgcg ccacgggatg
     7321 acgcatttcc cgccaccttt gggttattac tcggataacg gctcgggtga aacgggcgat
     7381 atgctggata aggaaacgac gggtattttg ccgcgtatcg gtatcgagca ttttacgggc
     7441 attccgggca atccgcaggg gcgcggcaag attgaacggc tgtggcagac cattacgatt
     7501 cctttggcca agcagtatgc gacttatcag ggcaaggatg cggatgccga aacattgaga
     7561 aaggtatcga atgcgctggc gagtgcgcag aaggtgcaga aaaagggcaa ggaactgagc
     7621 aaggcgcagg aagcggcgct gtcggcggca ccgacttggg cgcagtttat agcggatttg
     7681 gaagaagcgg tacgccgata caactttgaa cacgagcacc gcagcctgcc gaaaaatccg
     7741 gaagcgggac ggcattttac gccgatgaaa tattacgaat accggatgga aacggacggc
     7801 atgagggtga aaacggatgt attaagcccg ctggagttgg attttatgta ccgtccggag
     7861 gaagaacgca tccccgaccg cggagcggtg tctctgcaca ataatactta cttccaccaa
     7921 gaattgctgg attacagcgg tcagaaggta cgggtggctt acgacatcca cgatgccgac
     7981 catgtgattg tgaaggatat gcagggcaag gtgatttgca aagcggtatt caacggcaac
     8041 aaacgggcgg catttgcgga aacgcggatg gaacaactgg ccgaacgccg ccgcaaaggc
     8101 caggccaaac gtctgcaaga caagatggat ttaatcgagg cacaacgcca atcggccgcg
     8161 ccgattatcg agcagcagcc tgatttcggc gaatttttga agctggaaac ggaaagcggc
     8221 gggttggtag aggtggaaaa caggccgtct gaaagcggca ggggcaagaa gaagttacgc
     8281 gattttttat gggaagacgc cggttagtaa aacggcacat ctttgacact ttttaaggaa
     8341 aacgaaaaat gattgagaaa ttaagacaat ttctggaaac cagcggcatg agccaaaaca
     8401 aggcggccga ccgcatgggc gtatcgagaa gtgcattgtc gggctatttg aacggcaaat
     8461 acgacggcga tattgcgggg atggataaga aagccgcgct gtttcttgaa caggaaggcg
     8521 accgggcgga attgaaaaag ctggatattc cctatgttga aacgggcacg gccaagaaga
     8581 tgaaaggttg gctgggtttg gcggcatggc tggggcagct cggtattgtg tatggcggtg
     8641 cgggcttggg taaaaccacg gtattgaaac aatatgcggc cactaacccg ctggctttgt
     8701 tgattgagcc ggatacaggc tatacggcaa aggtgttgtt gcaggaaatc tgccatgcac
     8761 tggatttaag cgagagaggc aatatccatg agctgacgga gcggattatc aactgcctga
     8821 agcgtgataa gaagagccgc agcccgcgtg atgcacaccg tattttgctg attgacgagg
     8881 cggaacagtt accgacacgc gcactggaga gcctgcggcg gatacatgat aaatcgggcg
     8941 tggcggtggc gttggtgggt atgccaaagc tgctgctgaa tctgaagggg ccgaacagtg
     9001 aattcaagca gttgttttca agggtatcgg tcaagatgga attgggcgag atgctgccgg
     9061 aaacggattt aaaacagatt gcggcggcag tgttgaagac gaacgatgag gcggtattgc
     9121 agaaggtggt gaaaacggcc aaaggcaatg cgcggaaact gtcgaagctg ctgctgattg
     9181 tggactattt gttgcaggtt aaccctgatg ttgatttgga tgatgatgta atcgaacacg
     9241 cggaaaccta tttaatccac taaacctttg acagataagg caataatttt tttaccttgt
     9301 tattttgtta agttattgaa attaaaggaa aactaaaaat ggaaaatttg aaaatgcggc
     9361 atctgctcgc cataccggcg attgtgattg cggtaacggc gtgtgcgaac cgctgcaccg
     9421 agccggtgcc ggcgtggagc ctgtatcaga acgagaccag tcagcagcgg ctgatgcggg
     9481 aaaaacagaa tgcggctgac tttgcggtat tgcaggcaga aaaagcctat gaacggatga
     9541 gcgataaaga gcggatgaaa ggggtggttt atgaaaactg agacgaaaat ctgcaaaaaa
     9601 tgcggagaag agaggccgtt gttccgtttc aaaaaagacg atgcgtatgc gggcgggtat
     9661 aggcctgtat gttacgagtg tttaaacgcc agagcaaagg aactgaaggc agtccgagac
     9721 agtatggcgg aaagaaagat tatctctaaa ggcatcagca taaatggagg gaaaacgcat
     9781 atggaatttc ctgaagaacc tgaatttatg aaaaacaaat actggaccgt tacaaaaacg
     9841 gattatagaa gaccattaaa aggactttaa ccatgagtaa acggttggca cgacggaaga
     9901 tgccgaaaca cgaattgaaa cgggttaaag cgttcgccct gaaacaggcg gtcgagaaga
     9961 ttcgggcacg gtggggcgag aaagcgatac ggcttgcctc aacagacaac aaacagactg
    10021 attaaacaag gaataagaaa atgatgaatg aaatgaatgc ggtagcggct ttgttggtta
    10081 ttgccatatt gctggcggcg gtaatgactg tgttggtcga gatattggtc gagcaacggg
    10141 ttaaggaacg gctggggaaa ctgctggata aggcgtgccg accggatgac gggtggcagg
    10201 aatgataagg aaggaaatgt gatgaatatg gtactgacaa tttatttttg gttggtgggg
    10261 ctggtctgtt ttgcccgttc cgccaaagag acttattacg cggaatcttt atacggtctg
    10321 ctgactgggc tgttgggggt gatgtgttcg gcttttgtgg tgtcagtgtc ggtggcaagg
    10381 gtgatggggt ggttgaaatg aatcaattga aaatgacgat cgccaaacct gagacggaag
    10441 attttgaaga tgcctgggca tttatcagaa tgcttaattt ggtgacttat gatttgaacc
    10501 ctctgaaaac tgataccgac ggcgaatacg aatatttggc ggatgaagac aaatctgatg
    10561 tattagatgc cgttgtcgaa aaatttaacg aatgtagtct tgagtggatg ttgtcggcgc
    10621 tgcaagcact gatgtcgccg gagatgggga ttatcaacca agattcggat acgttggagt
    10681 tgcatcccaa actgaaaggc ggtacggaat gaaagtgcgc tgccctacct gcggtgcggt
    10741 gatgagctta gatgtattaa tcgcccatga tgatgcagcg aagctctgat tgccctgaca
    10801 ggcatttcag acgacctttt taaggcggta ttgcggtatc tgacgctgtt tcgccccgcc
    10861 gaaaaggatt taagttttaa ccgagtttca aagctcgtcg gcgagattgc gccgatgata
    10921 cgggacggaa agattgtccg caaccgcaaa acttatccgg ccccgcgcga ggcttggatt
    10981 tgggcggcaa cgcgatgcct tgaggcacgg gacgcgggaa aactgacacc gccactgacc
    11041 agccacggtt atttgttgga aaacattacg ttttggtcgc ctgaaaagac ggcgggaacg
    11101 gcggttttgc cctctcccca accatctcac acgagagagg tggcaagcac caaattgagg
    11161 agcgggttgg gcggcttgat ggagtggtca aatggaggaa aacaatagct ggctgaaaaa
    11221 agcaatcgcg cagggtttca tgatgctcgc cgccctaaac ctcaaaggcc gccctgcctc
    11281 ggcggatttg acggcagtcg ccgaactttg gttggtcata ctaagcggcc ggtcgtggca
    11341 gccggagcat gacgggatca ggatacaggc agcctttagg gctatcgcgg cgtcctcgtc
    11401 agagtggcca aaccctgccg accttatcaa gcacctgcca ccgggcgaag tcaggatggt
    11461 gccgaggctg gaaaagaagc actgcccgac ggaatacggc aaagagcagg ccgccgaact
    11521 gaaaaagatt gtcggcagat tgaaaaacgc accctgcatg aatagagatt ggatacacgg
    11581 gcaacgccac cggtcggtgg acgaatgtaa aaggatttat gccgaaaggc agaagggaaa
    11641 taaatagcca aatgagtacg ttttttgaca atcaggaatc cgacttaata aacgaatttc
    11701 tgacggaata ctgggaagag tttgaaaaat tctccgccaa caacggattt gatgacgatt
    11761 tattgaagca aatatcaatg gaattggaag gacataggaa ccattatgag taacttagac
    11821 atgaaacaat acaaacagga tgccaaaggc aatctcgtgc cgctggccaa tattaaagaa
    11881 atcgacctgc tgcgcgacga gctggtgcag gaaattgccg ccaaagcccg cgcggtacag
    11941 gataacttga tggcgttcaa acgcgaggcg atggacgata tcgcagcgtt tgtacagttg
    12001 agtgccgacc gctatgacgt atctgtcggc ggtaagaaag gcaatatcag cctgcacagc
    12061 tttgatggag cgtaccgcgt caaccttgcc atacaggaca cgttggtatt tgacgaaggt
    12121 ttgattgccg ccaaagccct gattgacgag tgtatcaacg aatggacgga aggcagtcgc
    12181 acggaattga aaacactgat taacgcggcc ttccaagtgg acaaagaagg caatatcagc
    12241 actgcccgcg tcctcggtct gcgccgcctg caaatcacgg atgaaaaatg gcaacgggcg
    12301 atggatgcgc tctccgacag tttgcaggtg catatcagca aaccgtttgt gcgggtatat
    12361 cagcgcggcg aggatgggga gtatcagtta atgaatttgg atgtggcgaa ggtgtaggaa
    12421 aaatgaacgc aaatataatg atttatttga ttgaagttaa tgatgggccg gttgcaaaag
    12481 cactgcgaag ttttcagcag gacaaggccg caatgcggca ggcgtggctt gattgggcgc
    12541 gagaacactt gccgtcagac gcgctaatgc gggaatggag cgatgggcaa gtggcagggt
    12601 ttgcgttccc ttcaggcata cccgatggat ggaaaaagcc caataagaac ggaatttgtt
    12661 ggccacggca aaacaaccca atcctcaaaa ccatgccact taacaaaaga tttaaacgcc
    12721 ccgaagagta tttggaagaa gttggcatca ccgcaccaac aatgattttt gaaaagaata
    12781 gcgacggaga gacttgggca tcttggggta tcggtaattt ctttaatcct gttcagttcg
    12841 tttgggcggg attggaggag gacgcgccaa agggcgtggt aactcctgat tatgcctacg
    12901 agcttagaga aggggctaag agaatcagaa acggctgtac gatgcagccg cccgaagatt
    12961 ttgattggca gaatctgtta ccgggctgcc gtgtcattcc tcggtatgag tgggattact
    13021 tggttgggaa atggcaggaa acacggaatg atgccgaaga acaggaggct taaatggcaa
    13081 aaatcattat tgaaatcgaa gacctgcccg agggcaccga cattaatttt aagggcgacc
    13141 ttccggcggc ggatgcaaaa gacaaaacag gtgcgcagca aacggctgtg ttaatcagca
    13201 agatgattca ggcggcgcag atgatggcat ccccatccgt aaacattagg ccgtctgaga
    13261 ctgcaaatcc ataacccgcg cggcacggtc tgccgcattt aaatctaaat aggagtcaaa
    13321 aagtgaataa atccgaatta atccaagcca tcgccgacga ggcggaattg agcaaacgcg
    13381 ctgcggcgga gtttgtcaat gcgtttgtca gcgtggtaac gcaagaactg aaagacggaa
    13441 acgacgttac gctggtcggc ttcggcacgt tccacgccgc ccaatccgcc gagcgtcagg
    13501 gacgcaatcc gaaaacgggc gaaccgctga ccatcgcggc gcgcaaaaca cccaagttcc
    13561 gctcgggcaa ggcgttaaaa gatgcggtaa acgtctgaaa ccccaaatcc aagcggattt
    13621 ttgaaaagtc cgcttcagtt tgaagtttta gcgacgagga aaaccatgat gaacacaaac
    13681 gaacagtaca aacgcaaggg gctgattgcg aaaatcaaaa tcgcgcaaag ccagcttggg
    13741 atggaagacg atgtttaccg tgcgatgctg gcgcgtgtgt gcggcaaaac atcctgcacg
    13801 cagatgaatc tagccgaatt gcaggcggtg gcggcggaaa tgaagcggat gggctttaag
    13861 cagaccgcac cgaagggcaa aggtatccgc ccgcatttga cacaggaccg tgccgcgctg
    13921 ttgaacaagc tggaagcgct gttgacggtg ggcgataaaa gctggcagta cgccgacggg
    13981 atggccaaaa ggatgttcgg caaggatttg gtacgatttt tgacacccga gcagctatac
    14041 aaactggtgc aggcgctgca aatccatatc aacaaagcga aaaaggcggc agagcaatga
    14101 tcagggtgga tgaagaagat tttgaggcgg tgcgccacct gctgcccgaa agtgtgctgg
    14161 cactgattac gatcatcggg ctgacagaaa cggtggagct ggtgaaaaac ttaggcggca
    14221 cgacctaccc gttgcggcag ggacgcacca aaggcagcga atcgcggctg gcttatttgg
    14281 aggaaatcgt cggcggtgcg gcgatggaaa aaatggtgga agcactggcc ccctgcgatt
    14341 tgtttattcc gaaatgcgag caggctttgc tggaattgcg tgaccgccat atccgccgcc
    14401 gcttcgatgc gcagacgggt aaaggcgtac cggcttatga ggcggtgaac gatttggctt
    14461 tggcgcacgc gctgaccgac cgccatatct ggcggattct gaagaagccg gataatgagt
    14521 gtgagcaagg cgggttgttt taaattataa tgccgtgggg ataacacggc attttttatg
    14581 ggaaatcaag atgaagacgt tttatgttct gatgttggcc gcaatgcttg cggcttgcgg
    14641 cggacaagaa aacaagcctg ctgccggcga aagtcaaacc gataatcggg cgcagcaata
    14701 tcagaatcag caaaaacaga ttgccgcacg tttggctgat gaagaatatt tcatcggtga
    14761 aaacaatttg gaacaaatcc gtctgcacgc caatttgaaa gagcgcgctc aaaagctgtt
    14821 gtctttattg gcgcaggccg acaaggaaag ccgtgtgtgg gttttgcccg gcgatgcagc
    14881 caaaatcaaa gaattcaatg cggctttcgc ggcggtggca aaatctgccg aggaatcttt
    14941 cggcggccct tttcttgcgg ataaggcggg gctttatcaa tgtaccgctg cggccaatgc
    15001 ggcttacgat tattttttcg cccggcagag aaaggatgcg ctgaccgaaa attaccgtca
    15061 acaatacacg gacaacatag ctgcctgcca agggcaaatc cgcaccccgc cagccgccca
    15121 agcgacagtg tatgcgcgta aaggtatcag gctgccgttg aataattgtt tgccggtatt
    15181 ggccggagat gaagaatttg atacctacac ctgcccgatg gaagtaaaat aggccgtctg
    15241 aaacccagcc cgagagcaaa agccctcggg ctttttgctg cctgtctgac atggggcagg
    15301 cgcggtgcca aataagcgga caggataatc aaggctcgat aacagactaa gcaggacgaa
    15361 tctatgagca agattattgt gctgaccgca ggccacagca acaccgaccc gggtgcggtc
    15421 aacggaagcg accgtgaggc ggacttggcg caggatatgc gcaacattgt ggcttcaatc
    15481 ctgcgtaacg attacggcct gaccgttaaa accgacggca cgggcaaagg caatatgccg
    15541 ctgcgcgaag cggtcaagct gattcgcggc tcggatgtgg cgattgagtt tcacaccaac
    15601 gctgccgtca gcaaagcggc gacaggcatc gaagccttga gtaccgttaa aaacaaacgc
    15661 tggtgtcagg tgttgagcaa agccgttgcc aagaaaaccg gctggaaact gcgcggcgaa
    15721 gacggcttta aacccgacaa tgcgggccag cattcgcgcc tggcttatgc acaagccggc
    15781 ggcattgtgt ttgagccttt tttcatcagc aacgacactg atttggcctt gtttaagacg
    15841 actaaatggg gcatctgccg cgcgattgcg gacgcgattg cgatggaatt gggggcggca
    15901 agagtatgaa tattattggt aaattgaaag aagctgcttc ctattttctt acaaaattga
    15961 ttggagaaaa tcctagtaat gagcaggtaa accgcgcact tatacagatg ccaaatgttc
    16021 gtccgataca cacctatcca cgcccaaatt taagaaactc aggcgtggca gccgcgaaac
    16081 gcgcggcgcg caaacgcaag aatcgtcgtt aatcatggga caggttgcgt tttacgaaaa
    16141 gatgattggg ctgtggtcgg ccaaaagccg tgaggcaagc gaacaggcag acttggcggc
    16201 gtttgaattt gcggagggcg aactggccaa ttatcaggaa atgctgaaac ggcacctgca
    16261 aaccaaaagt gtggaatagc aatgcgtatt ttggatattt ttaaaaaccc agcgacaggc
    16321 aatgtgtcgc actcgaaact gtgggcaaac gttgcctgcg cggcggggac ggttaagttt
    16381 gtgatgctgc ccgacccgtc ggcggagatt tgggcggtgt atttgggcat tgtcggcggc
    16441 tatgcggtgg cgcgttcgtt ggtcagcgtc aaacgtcagg aggtcgagaa tgaatctcgt
    16501 gaaactgctg gcgaataact ggcaaccgat tgccatcatc gcgcttgtcg gcacgctctt
    16561 ggcggtgtcg caccatcaag gctacaagtc ggcttttgcg aagcagcagg cggtcattga
    16621 caagatggaa aaagacaaag cgcaagccct gctgttgtcg gctcaaaact acgcccgcga
    16681 gctggaacag gcgcgtgcgg aagctaaaaa atatgaagtc aaggcgcacg ccgtcggcat
    16741 ggctttggcg aaaaaacagg cggaagtcag ccgtctgaaa acggaaaata aaaaggaaat
    16801 cgaaaatgtc cttactcaag accgtaaaaa tgcaggcggc ggttgtattg acggctttgg
    16861 ccatcacggc ttgcagctct acaagcgcgc cctcggctac ggaaattaag gttgtcgaaa
    16921 aggcggtcat gccgacaccg cctgccgcgt tgatggtcgc gccggtgcgc ccgaatccgc
    16981 cgaaagacgg caagacggcc acgctgttgg aacacgccgc cgagtttggc ggctatgttg
    17041 ccgaacttga aaaccaaa
//
LOCUS       DAWKOD010000027        16840 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000027 DAWKOD010000000
VERSION     DAWKOD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.027"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..808
                     /locus_tag="WJK73_000692"
     CDS             <1..808
                     /locus_tag="WJK73_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7557211.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKVIRGNV"
     gene            849..1379
                     /locus_tag="WJK73_000693"
     CDS             849..1379
                     /locus_tag="WJK73_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ7557212.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1390..1529)
                     /locus_tag="WJK73_000694"
                     /pseudo
     CDS             complement(1390..1529)
                     /locus_tag="WJK73_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1537..3132)
                     /locus_tag="WJK73_000695"
     CDS             complement(1537..3132)
                     /locus_tag="WJK73_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ7557213.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3239..3634)
                     /gene="hisI"
                     /locus_tag="WJK73_000696"
     CDS             complement(3239..3634)
                     /gene="hisI"
                     /locus_tag="WJK73_000696"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ7557214.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3665..4432)
                     /gene="hisF"
                     /locus_tag="WJK73_000697"
     CDS             complement(3665..4432)
                     /gene="hisF"
                     /locus_tag="WJK73_000697"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ7557215.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4445..5182)
                     /gene="hisA"
                     /locus_tag="WJK73_000698"
     CDS             complement(4445..5182)
                     /gene="hisA"
                     /locus_tag="WJK73_000698"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ7557216.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5214..5852)
                     /gene="hisH"
                     /locus_tag="WJK73_000699"
     CDS             complement(5214..5852)
                     /gene="hisH"
                     /locus_tag="WJK73_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ7557217.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPEDPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5983..7485)
                     /gene="pta"
                     /locus_tag="WJK73_000700"
     CDS             complement(5983..7485)
                     /gene="pta"
                     /locus_tag="WJK73_000700"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ7557218.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7670..8728)
                     /gene="fbpC"
                     /locus_tag="WJK73_000701"
     CDS             complement(7670..8728)
                     /gene="fbpC"
                     /locus_tag="WJK73_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ7557219.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLQVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8749..10266)
                     /gene="fbpB"
                     /locus_tag="WJK73_000702"
     CDS             complement(8749..10266)
                     /gene="fbpB"
                     /locus_tag="WJK73_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ7557220.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10334..11329)
                     /gene="fbpA"
                     /locus_tag="WJK73_000703"
     CDS             complement(10334..11329)
                     /gene="fbpA"
                     /locus_tag="WJK73_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ7557221.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11580..12050)
                     /locus_tag="WJK73_000704"
     CDS             complement(11580..12050)
                     /locus_tag="WJK73_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ7557222.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAKADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12108..13484)
                     /gene="argH"
                     /locus_tag="WJK73_000705"
     CDS             complement(12108..13484)
                     /gene="argH"
                     /locus_tag="WJK73_000705"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ7557223.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASISFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13503..14372)
                     /gene="galU"
                     /locus_tag="WJK73_000706"
     CDS             complement(13503..14372)
                     /gene="galU"
                     /locus_tag="WJK73_000706"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7557224.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQSAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14400..14999)
                     /gene="rdgB"
                     /locus_tag="WJK73_000707"
     CDS             complement(14400..14999)
                     /gene="rdgB"
                     /locus_tag="WJK73_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ7557225.1"
                     /translation="MSEKPEKIVLASGNVGKLEEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHVAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(14992..15225)
                     /locus_tag="WJK73_000708"
     CDS             complement(14992..15225)
                     /locus_tag="WJK73_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557226.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVAAFLFA
                     FGALFGQIGALALYLRHKSLHAAQSAAKENRYV"
     gene            complement(15337..15870)
                     /locus_tag="WJK73_000709"
     CDS             complement(15337..15870)
                     /locus_tag="WJK73_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ7557227.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15972..16430)
                     /gene="nudB"
                     /locus_tag="WJK73_000710"
     CDS             complement(15972..16430)
                     /gene="nudB"
                     /locus_tag="WJK73_000710"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ7557228.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16641..16717
                     /locus_tag="WJK73_000711"
     tRNA            16641..16717
                     /locus_tag="WJK73_000711"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16675..16677,aa:Pro,seq:ggg)
BASE COUNT         3482 a         4044 c         5121 g         4193 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttaa tagggatata tcaagttatc tttattcctt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcagaat
      781 tttaaaagta atcagaggga atgtttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaaggat
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gaatcggcaa ccgcgtcttg gtcggtatgg gcagcaccgt tttggacgat gctgtgattg
     1201 aggacgaagt gatgattggc gcgggcagcc tcgttccgcc gcgcaaacgc ttggcgggcg
     1261 gctaccttta tatcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg acgggatgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaagcccttt tttcgggttt agacggcatt ttatcgttac agtttgaccg aatgttcgcg
     1561 cgtttcgtgg aacacgatgt ccggccaacg ttcttgcgtg agtcccagat tcacgcggtt
     1621 aggggcgagg taggcgaggt tgccgcccgc gtcgatggcg aggttgcccg cgttggcttt
     1681 ttcaaattcc gccagttttt tcttgtcgtc gcacgatacc cagcgcgccg accagatgga
     1741 tgcgctgtcg aacacggctt ctacgccgta ttcgttggcc agccgcgagg taacgacttc
     1801 aaattgcaac acgccgaccg cgcccaaaat caaatccgcg ccgctcatcg gtttgaaaac
     1861 ctgtaccgcg ccttcttcgc cgagttgttg caaacctttt tgcagttgct tgattttcag
     1921 cgggtttttg atgcggacgc tgcggaacag ttcgggcgcg aagaatggga tgccggtaaa
     1981 cgtcagttgt tcgccttcgg agaagctgtc gccgatttgg atgttgccgt ggtttgggat
     2041 accgataatg tcgccggcgt aggcttcttc aaccagctcg cggtcgtggg acatgaaggt
     2101 taccacgctg gaggcggcga tttcgcggtt gatacgcagg tgtttcatct tcataccgcg
     2161 ctcgaatctg ccggagcaga cgcgcaagaa ggcgatacgg tcgcggtgtt tcggatccat
     2221 attggcttgg attttaaaga taaatccgga aaacttagcc tcgtccggct cgaccatacg
     2281 tacggtcgcg tcgcgcggtt tcggcgcggg cgcccagtca attaatgaat tgaggatttc
     2341 ctgaataccg aagttgttaa tcgcagagcc gaagaatacg ggcgtgagtt cgccggcgag
     2401 gaattcgtcg agattaaact cgttcgatgc tgcctgcacc aattcgattt cgtcgcgcaa
     2461 ctgttggatt tccaatggaa agcgttgttc caattcggga ttttctatgc ctttgatgat
     2521 gtcgaactcg tgcggcaggc gttcgccgcc cgcttcaaag agatagattt cgtcgttcag
     2581 gatgtggtat acgcctttga agtttttgcc cataccgatc ggccaggtaa cgggcgcgca
     2641 gcggatttgc aggatgtttt ccacttcgtc caagagttcc agcgaatcgc gcacttcgcg
     2701 gtcgtatttg ttcataaacg taacaatcgg cgtatcgcgc aggcggcaga cgtttaagag
     2761 cttgatggtt tgcgcttcca cgccttttgc cgcgtcgatg accattaatg cgctgtccac
     2821 ggcggttaaa acgcggtagg tgtcttcgga gaagtcttgg tgtcccggcg tgtccaagag
     2881 gttgacggtg tggtctttat agtcgaactg catcacgctt gatgccacgg aaatgccgcg
     2941 ctgcttctcg atgtccatcc agtcggaggt ggcgaatttg ccggttttct tgccttttac
     3001 cgtacccgcg ctttgaatcg cacctgaaaa cagcaagagt ttttcagtca acgtggtttt
     3061 acctgcgtca gggtgggaga tgatggcaaa cgtgcggcgg cggcgcactt ggtcgaggat
     3121 ttcttgggac atggttttct ttgcaaaaag gttcaggccg cttttcagac ggcccggaca
     3181 gtgtttgaga cggcgaaatt gtacaaaaaa atgcctgata attcaatgtt ggaggcggtc
     3241 agtgcgtgct gccgtaaatc tctttttcgt ctttcaggac ggcatcggcg gtttcccacg
     3301 cgctgccacg ccagactttg taaaagcagc tttctcgccc ggtgtggcag gcgatgccgc
     3361 cgttttgggc gatgagcatc acaatggcgt cgccgtcgca gtcgaggcgc agttcgcgga
     3421 ctttttgcgt gtgtcccgac tcttcgccct tcatccattg tttttggcgc gaacggctgt
     3481 aatagtgggc aaagccggtt tcgacggttt tttgcagggc ttcggcgttc atccacgcca
     3541 ccattaaaat acgtttggtt tcggcatctt gggcgatggc gcaaaccaaa cctttttcgt
     3601 caaatttgac ggcttcaagc aggtttttat ccatatttcc tttcagacgg catggtcgag
     3661 gcggtcagag gcgcacttcg atgccggctt cgcgcatagc gcgtttggct tcgcggatgg
     3721 cgatttcccc gaaatggaaa atgccggcgg caagtacggc atcggctttg ccttcggtta
     3781 tgccttcaat caggtgccgg acattgccga ccccgccgga ggcgatgacg ggaatgtcga
     3841 cggcttcggc aacggcgcgg gtcagcggca ggttgaaacc ctgtttcgta ccgtccctgt
     3901 ccataccggt aagcaggatt tcgcccgcgc cgcgtttttg catttcgatc gcccattcca
     3961 ccgcatccaa accggtcgga ttgcgcccgc cgtgggtaaa gatttcccag cgtgtgtttt
     4021 cggggttgac ggctttggca tcgacggcgg cgacgatggc ttgcgaaccg aaaaatccgg
     4081 cggcttcgtc aatcaggtcg ggacgggtaa cggcggcggt gttgatgctg actttgtccg
     4141 cgccggcatt gagcaggcgg cggatgtcgg caacggtgcg tacgccgccg ccgacggtca
     4201 gggggatgaa gacttgtccg gcaacctctt cgatgatgtg caggatggtg tcgcggttgt
     4261 cggatgaggc ggtgatgtcg aggaaggtca attcgtccgc gccttcgccg ttgtagcgtt
     4321 tggcggcttc gacagggtcg cccgcgtcgc gcaaaccgat gaagttcacg cctttgacga
     4381 cgcgcccgtc ttttacgtcg agacagggga tgatgcgttt tgccagtgcc ataatcggat
     4441 gcctttagtc gagggaatct gccagttgct gcgcttgggc aaaatcgatg ctgccctcgt
     4501 aaatcgcgcg gccggtaatc gcgcctgcta cgccatgttt ttcggcggca cacagggcgc
     4561 ggatgtcgtc caagccggtc agtccgccgg aggcgatgac gggaatgcgg acggcttggg
     4621 cgagtttgac cgtcgcgtcg atgttcacgc cgctcatcat accgtcgcgc ccgatgtcgg
     4681 tgtagatgat gctgttgacg ccgtcgtctt caaagcgttt tgccaaatca attacatgat
     4741 gcccggttac ggttgcccag ccgtcgatgg cggccatacc gtctttggca tccagcccga
     4801 caataatcct gccggggaag gctttgcacg cctcgcgcac gaagtcgggg tttttgaccg
     4861 ccgccgtgcc gataatcacg tcgttcaggc ccaaatccaa atattgtccg atggttttca
     4921 aatcgcgtat gccgccgccg agctgtacgg ggatgtcttt ggcaacggcg gcaaggatgt
     4981 ctttgatggc gggcaggttt tgcggaacac cggcaaacgc gccgttcaaa tctaccagat
     5041 gcaggcggcg cgcgccttgt ttgaaccagt gcagcgcggt ttcggcaggg gagtcggaaa
     5101 agacggtcgc ctcttccatc agcccttgtt tcaggcggac gcagcgtcct tctttcaaat
     5161 cgatggcggg tatcagcagc atagtttttc tccttgtggg gggcgtgtcc ggcttaccag
     5221 tttaaaaagt ttttcaacat cgtcagcccg gcatcgtggc ttttttcggt gtgaaattgt
     5281 gtggcgaata cgttgtcttt gccgacgatg caggcaaacg gggacgggta gtcgctttcg
     5341 cccaatatgg tttcgggatc ttcgggggcg aaatagtagc tgtggacgaa gtaaaaacgc
     5401 gtgtcttggg gaatatcttt aaacagcggg tggttttggg tttggcgcac ggtgttccag
     5461 cccatatgcg ggactttcag acggcatccc tgccggtcgc ggaggtcgcg ctcaaagcgt
     5521 ctgactttgc cgccgaacca gcccaagccg tcggtgtttc cttcttcgct gtggtcgaat
     5581 aaaagttgcg cgccgacgca gattccgaaa aagggtttgt tttttaaggc atctttgact
     5641 gcctcgtcca agccgccgcg cgtcagtgcc gcaatacagt cgggcatcgc gccctgcccg
     5701 ggaaaaatga ctttgtcggc gcgggacacg cgttcggggt cgccgcttaa aaagatttcg
     5761 gtattttttc cggcaagctg ccccgccgtc cggacggatt tcaatacgga atgcaggttg
     5821 cccataccgt aatcgataat ggcgatttgc atggcttcct cctctttttt tgcaatatgg
     5881 ctgcgatttt aacaaacaaa tgtgccgtgc tgataaaaat gccgtctgaa aacgggagtc
     5941 tgtcttcaga cggcataggg tttaaacccg gaaagccgtt tgtcagcctt ccatttgttt
     6001 tgcctgaacg gcagttaggg cgatggtgaa cacgatgtct tccaccagcg cgccgcgcga
     6061 caggtcgttg accggtttgc gcaggccttg cagcagcggg ccgacgctta agacgttggc
     6121 actgcgttgg acggctttat aggtgcagtt gccggtgttc aggtcgggga agaccaaaac
     6181 ggttgcctgt cctgccaccg ggctgcccgg agctttggat ttgcccacac ccggcacggt
     6241 tgccgcatca tattgcagcg ggccgtcgat ggcgaggtcg gggcgttttt cccgggcaag
     6301 tttggttgct tcgatgacga tatcgacatc ggggccgctg ccggagttga cggtggagta
     6361 ggaaatcatc gccactttcg ggtcgatgcc gaaggctttt gcggaatcgg cagactggat
     6421 ggcgatgtcg gcaagctgtt gcgcggtcgg gttcggatta accgcgcagt cgccgaagac
     6481 gaggacttgg ttgggcagca gcataaagaa tacgctggac acgaggcttg cgcccggtgc
     6541 ggttttaatc agttgcaaag cggggcggat ggtgttggcg gtggtgtgaa ccgcaccgga
     6601 taccaaaccg tccacatcat tttgcgccat catcatcgta ccgagtacca cggtgtcttg
     6661 cagttgcttg cgcgcgtctt cgggtgtcag gcctttggat ttgcgcagtt cgcacatcgg
     6721 tccgacgtat tgttcgacca atgaggcggg atcgatgatt tccaaagagt cgggcaggct
     6781 gataccgcgt tctttggcaa cggcttcgac ttcttcgcgt ttggcaagca ggacgcagcg
     6841 ggcaatgcct ttttcgtggc agatggcggc ggcttggacg gtgcggggtt ctgcgccttc
     6901 aggcaggacg atgcgtttgt cggcttggcg ggcgaagtcg atcaggttgt agcggaattg
     6961 cgccggcgac aggcgttttg cttcgcggcc tgccaatacg gatacgtctt tcagcgcgtc
     7021 gctcgaaccg aagaaggtca ggccggtttt ttcggctgcc gcttcggcaa cggaggctgc
     7081 cgcgccgtcc acgacaaaac cttccaatac gcccggcgcg gcggcgaaga actgtttggc
     7141 aaggttcacc cgatttgcca gttcgtcggc atcggcgttg tcggaacgga cggcgaagac
     7201 ggctgccgcg tcaagggaca atgccagttc gacgtttttg cctgcgaggt agattttgtc
     7261 ggcatcgggc gcgatgcctt cgatgacgag gttggcggca tcgagtgcgg caactttgcc
     7321 gaccagtgcg tcgaaccagt cgtcgctttt gccttgcgcg agcagggttt cggcggttgc
     7381 gtcaacggct tggaaaattt gtgcgtccag tgcttttgca aaggcttgtg cggcggcgga
     7441 ggcgtccagt ccggcagata cgggtacgat gagtactttt gccatgatat atcctttcgt
     7501 atgctgcggt gtgcggcata tgtggttgga aggggcggca tataggcaga aacggctgcc
     7561 tgcgtgccgt gcgtgccgtg tttggcttga ggcgcgcagg ttgaatatag caaacaaatt
     7621 ctgtttccaa caagataaat atccgcaggc ttgtggatgc tgccgccttt cagagggtat
     7681 ttccggggaa gaacagggcg ggaccgtcca aatggaggac ggcggaaatg ccgtctgaca
     7741 gggtgggggc ggaagggagg ttgagcgtga ggacggtttg tccgacctgg agactgattt
     7801 cggtatgccg cgctttgggc gtggttttga gaaccacggc gtgaatggag gcggtgggtg
     7861 cggaatgggg gtgaaggctg aactgttccg gacggatgag cagtgtgccg cgtgtgcctg
     7921 cgggtgcgcc gctttgaacg ggcaggcggc ccaatccgca atcggcggtg ccgtcggcgt
     7981 tgagcgcggc ggggaacacg atgccttcgc cgataaacag ggcggcatca aggtcggcag
     8041 gttgtcggta caattcgtga gggcttgcag tttggaggat gcgcccctgt ttcatcacgg
     8101 caatccggtc ggcgtattgc agggcttctt cgcggtcgtg gctgacgaaa acggcagatt
     8161 tgccgttggc gcgcagggcg gcaatcatgt cttcgcgaat ctggcggcgc aactgttcgt
     8221 ccagcgcgct gaagggttcg tccaacaaaa tcagttcggg atcgggtgcg agggcgcggg
     8281 cgagggcgac gcgctgttgc tgtccgcccg aaagttcgtg cggatagcgt ccggcaagtt
     8341 cggaaatgcc ggtcaattcc aacatagctt cgatgcgctg ccgctcttgc gccgtcttgc
     8401 ctttgccgtt gcccagcccg taggcggtgt tgcggtaaac ggtcaggtgg gggaacagca
     8461 caccttcctg tacgacataa cccaaacggc gttcgcggac ggggaggttg gtatttttcg
     8521 agaagatggt tctgccggaa agcgaaattt cgccaaaatc gggttgttca aaaccggcaa
     8581 ggcagcgtaa aagggtggtt ttgccgcagc cggacgcgcc gacgataaag aggatttcgc
     8641 ccgggtcgag gctgagcgaa atgtcgttta aaaccggggt gttttgaaaa cttttggaca
     8701 ggtgtccgat gtgcagggcg gcggtcatgg cggtacttcc tcaagctgtt atttgaaggc
     8761 gtatttcttc agcaggaata cggggatgcc ggaaaataat accagcatca gcgcgtaagg
     8821 ggtggcggcg gcgtattgtg cgtctgatgt gtattcccaa acggcggtgg agagtgtgtg
     8881 gacatcgtcg gtggtcagca gcagggtggc ggtcagctct ttcatcagtt tgaggaagac
     8941 gagtgcgaat gcggcggtaa tgccgggcag gatggacggc agtaccaacg tcctgaaaat
     9001 aaagaagtgt ccgcgcccca atgttgcgcc gacctgttcc atcccttttg ggagttgttc
     9061 caaggaagtc ctcagggtgg tttgcgccat cggcaggtaa agcatgaaat aggcaaggat
     9121 gacgacgata aaggtttggt aaacggcagg ggtgtagttg atgctgaaat aaaccaagga
     9181 tagggcgata accaaaccgg ggacggcgtg cagtaaaaac ggcagcctgt ctatccaaac
     9241 ggttaaaaaa ttgcgatagc gaaccgatgc ccaaacaagg ggcaaggcac ataatatagt
     9301 caaaatcgca cctaaagccg atacgcttaa ggaacggata aaggcatcaa atacggatac
     9361 gagcgcgaat gtgccggaag tgccgaccat catccaatgt atcaatacgc caaaggggat
     9421 aataatgccc aaagtcaaca agctgcttaa aaaaacaatc gcgccgatct gaccgggcag
     9481 tttgagggtt ttgacgggat aaggacgggc aacgcctttg ccgctgtggt aaatcttggc
     9541 tttgccgcga aatatgcttt ctccaaatac gacgatgccg cacaccgcca ttaaaacagc
     9601 ggaaagcagg gcggcggtat tgttgttgta ggacatttcg tattcttgga aaatggcggt
     9661 ggtaaaagtg gggtagttca aaatggatac cgcgccaaat tcgaccaaca tatgcagggc
     9721 aatcagtaac acgctgctgc cgatggcggg tttgagctgg gggaggatgg cggaaaaaaa
     9781 ggtttgcagg cggcttttgc ccaaggacag gctgacttct tcgtaagaca ggctgatgcg
     9841 tttgagtgcc gcctcgacgg gcaggtaggc gagcgggaac gaggacaggc tcataatcat
     9901 cactgtcccc caaaagcctt cgacacggaa ggtcaggctg atccaggtga aacagctgac
     9961 aaatgcgggg atgcacaaag gcagggtgat tgccgtctga aaaaaggttt tgccgaagaa
    10021 gcggtaacgt tggaacaaaa gggcgcaggc aatgcccaaa acaatggaaa tcagggtaac
    10081 gcccgccatc atcgtcaagg tgttggagag caaatcccac atacgcgggc ggaacaacag
    10141 ttcgacggcg cggttgatgc cgacctgcca cgaacgcata gcgacatata aaaaaggcag
    10201 ggtaagcggt agggcaatca gtaggatgag gccggtaagc caaatgggta tttttttagg
    10261 agacatagtg tttttcatcg gcaaaacggg cggacagtat aaatgtccac ccgtttgaca
    10321 atccgaaaac ggcttatttc ataccggctt gctcaagcag ccgggtggcg tgttcttttt
    10381 cggaaacagt ggtggcggac acttggggtg cttccaactt ggcgatgggt tccaaattga
    10441 aagtggatac cacgtgcgga ttcaaaggat attcggcacg gacggcggtc agggcgcgct
    10501 gtccttcctt gctggcgagg aaggcgacga attttttcgc ctcatccttg ttttgggagg
    10561 attttaacac ggctgcgccg gaataggtaa cgagtgcgcc gggatctctg tggcggacga
    10621 aattcaggcg ggtgtggaca ttttgtacgc ctttttcacg cgcaaaagcg tgccagtagt
    10681 agttgttgat gagggcggca tcgatttcgc cgttttcaac cgcttgaagg gcgacggagt
    10741 ttttagcgta aggcttgccg tattctttca gacctttgag ccatttcaat gcggccgctt
    10801 cgcctttcag tttgacgatg gcgacaacct gttccaagaa cgcgccggaa gtgggggcgt
    10861 aaccgatgcg gtttttccat ttcggcgtgg cgtaattcag gacggatttt tccaaatctt
    10921 tttcagacag tttgcgggtg tcgtaaacga cgacgcgcga acgtccgctc agtgccaccc
    10981 agtctttttt ggcggcaacc ggcacgccct tgccgcgtgt ttcgttgatg gtggaggcgg
    11041 gcaggggctc taggaggttg gcggcggaaa gggtggcgag tgccgggatt tgttcggaat
    11101 agaatacgtc ggcggggctt cggctgcctt cttctttgat ttgaccggca agctggtcgc
    11161 ctttggcgct gttgagtttg actttgatgc cggtagcccg ggtaaaggca tctgcaacgg
    11221 cttgtgctgc ttctttgtgt tggccgttgt acacggtaat gtctgccagc gcgggggttg
    11281 cggcggtcag ggctgcggca agcagtgcgt atcggataga tgttttcata tcgattttct
    11341 cctaatgaat gagagtgtac accttgttaa aacataacgg tgtgtagtgt attccttctt
    11401 ctttataaat gcaaataatt attttttaaa tttgttatta tccgatccgg ttattgtttg
    11461 ttctgacttg tattttttcc gtgcatcgcg cccgtaaggc ggaagcggcg ggcaatgcgt
    11521 ggcggaatgt gggtaaaggc ggcattttga tttgtcggaa tgcttgagaa cccctctctt
    11581 taaaacagcg tctgaacagg gttgccggaa tagtattgcc atcccggcag atacagtttg
    11641 tcggggtctt gccaatattg ttcatccaga ctgttcggca gtgaggcggt tttgttgtcg
    11701 agatgttttg ttatatccca catttctttc aggtttttac cttccgattg gaggtggcgg
    11761 atttcttttc tcaatgcgtc tgccgcccga tggatcagca ttgcgctgtg tatcgcgccg
    11821 atttttttca gaacggaaca agtcttttcg gcggcgggaa aaccccaatt gcagacaaat
    11881 tgcagtatgc cgccgttgcc gatgtcggct ttcgcccgcc aaatcaaaac cagctcctgt
    11941 tcccgcccgt ccatgctttc gagtttgccg tcatgctgtt caaaaagttt gtccaccgcc
    12001 tgccacatca tctgttcgaa gcggtcggct tgggtatccg tatcggtcat cgtgttcttg
    12061 ccttttaaaa atgccgtctg aacatttctt cagacggcat ttgggggtta agccaacatt
    12121 tcccgccagc gtttgacttg gaaacggact tgttcaggcg cggtgccgcc caagtggttg
    12181 cgcgcgttta agctgccttc gggtgtcagc acgccgtaaa cgtcgtcggc aatcaaatcg
    12241 ctgaaacctt gtaagacttc gagcggcagt tcgctcaaat cgacgcccgc ttggtcggcg
    12301 tggcgcacgg cttgggcgac gacttcgtgg gcatcgcgga aaggcatgcc ttttttgacc
    12361 agataatccg ccaaatcggt ggcggtagcg aagccctgca tcacggcggc gcgcatattg
    12421 tcgggtttga cggttacgcc gcgcatcata tcggcgtaaa tccgcaacgt gtcgataagc
    12481 gtgtcggcgg tgtcgaacaa gggttctttg tcttcctgat tgtctttgtt gtacgccaag
    12541 ggctgcgatt tcatcagggt aatcaagccg ataaggtgtc cgatgactcg gccggatttg
    12601 ccgcgcacga gttcgggcac gtcgggattt ttcttctgcg gcatgatgga cgaacctgtg
    12661 cagaaacggt cggcgatgtc gataaagccg aaacgcggac tcatccacaa aatcaattct
    12721 tcagacaggc ggctcaggtg aaccataacc agcgaggcgg cggcggtgaa ctcgatggca
    12781 aaatcgcggt cggatacggc atcgagcgag ttttggcaga tttgttcaaa gcccaacagt
    12841 ttggcggtaa tttcgcgccg aatcggataa gtcgtaccgg caagggcggc tgcgccgagc
    12901 ggcatacggt tgacgcggcg gcggcagtcc gccatccgtt cgttatcgcg tccgagcatt
    12961 tcgacgtagg cgagcatatg gtgtccgaag ctgacgggct gggcgacttg caggtgggta
    13021 aagcctggca taacggtttc ggcgttttgt tccgccaaat ccagcaatgc cgtctgaagg
    13081 ctttgaatca ggcttcggat gacggtaatc tggtcgcgca gccaaaggcg gatgtcggtg
    13141 gcgacttggt cgttgcggct gcggccggtg tgcaggcgtt tgcccgcgtc gccgattttg
    13201 tcggtcaggc ggcgttcgat gttcatgtgg acatcttcca aatcggacga ccattcgatt
    13261 ttgccgctgc ggatttcttc gaggatttcc gccatgcccc gacggatgtc tgccaaatcg
    13321 ccttcgtcca acacgccggt ttctttcaac atttgcgcgt gggcgagcga gccttgaatg
    13381 tcccattcgg caagccgtcg gtcgaaactg atggaggcgg tgtattgttt gacgagttcg
    13441 gaaacgggtt cgttgaaacg tccggaccag gttttgtcgt gcataaggat tccttgatgg
    13501 ggttattcgg tgcggtattt ttccaaaagc cggcggaagg gttcgccggt ttcgggatgt
    13561 ttcagaccgt aggcgacggt ggcttcgagg tagcccagtt tgctgccgca gtcgtagcgc
    13621 gtaccttcaa aggggtgcgc caggacaaat tcgtgatcga gcagcttggc gatgccgtct
    13681 gtaagctgga tttcgttgcc cgcgccgcgc ggaagattgg ttaagaggtc gaaaatgcgc
    13741 ggcgtgagaa tgtagcgtcc gacgacggca aggttggagg gcgcgtcttc gggcttgggt
    13801 ttttcgacaa tgccggtaat gcgttggaac tgtttgagct gttcggtttc gacgatgccg
    13861 tatgagccgg tttgcgatgg ttcgacggtt tctacgccca aaatgctgtt gccgctgcgt
    13921 tcgtacactt cgaccatttg tttgagcgcg ccttttgggg catcgattaa gtcgtcagcc
    13981 aaaatcacgg caaagggttc gtcgccgatg gcggcgcggg cgcacaagac ggcgtgtccc
    14041 aagcccagcg cttccgcctg acggatgtag aggcaggtga tgttcggcgg caggatgttg
    14101 cggacgtgtt ccaacaattt gtctttatgg cgcatttcca actcggtttc gagttcgtat
    14161 gccttgtcga aatggtcttc gatgctgcgt ttgttgcgtc cggttacaaa caccatttcc
    14221 gtgcagccgg cttccacggc ttcttctacg gcggattgga tcagcggctt gtcgacgatg
    14281 ggcagcattt ctttcgggct tgccttggtg gcgggcagga agcgtgttcc catccctgcg
    14341 acggggaaga cggctttttt tatcggtttc attctttttc ctttgtattg ttttgatgtt
    14401 taaagggcga gtttgcgaat taaatcggct agtgcctgcg cgcggtggct ttcgcggttt
    14461 ttgacctccg aatccaattc ggcggcggtt ttgccgtgtc cgggcagata aaaatacggg
    14521 tcgtaaccga aaccgttttc accggcggga acgccgttcc attgtccgtg ccatacgcct
    14581 tcggcaataa tcggacgcgg gtcgtcttta tggcggacaa aaaccaatac gcagacgtag
    14641 caacagcttt tgtcggcttt gccggcaagt tcggcggcaa gtttcaggtt gttggcggta
    14701 tcggatttgg gattgctgcc ggcgtaacgt gcggaatgga tgcccggagc accgtttaag
    14761 gcggcggcac agatgccgct gtcgtcggcg agtgcgggca gcccgctgtg tccggcgacg
    14821 tgccgggctt ttgccagcgc attttcaaca aaggtggaat agggttcggg gcattcgggt
    14881 atgctgaact cggattgcgg caatacggta atgccgtatg gttcgaatag gttgccgaac
    14941 tcttcgagct tgccgacatt accgcttgcc aaaacgattt tttccggttt ttcagacata
    15001 gcggttttcc tttgcggcgg attgggcggc gtgtagggat ttgtgccgca aatagagggc
    15061 gagtgcgccg atttgtccga acagcgcgcc gaatgcgaaa aggaaggcgg cgacggcaaa
    15121 ggctttgctg ccggcggtca gcagataagc acccaagagg acgagcagca taaatgccag
    15181 tgtgaacagg gcaacagaca acagatagat ttttcggcgg ttcatggcgt tcggtcggaa
    15241 acggtatgtt cggattatat ccgattggga cggtattccc tagagcttgg aaaaatgccg
    15301 tctgaagcgg ctttcagacg gcagggcagg cggatgttat gcctgtttgt tccaacgttc
    15361 gatggattct ttaataactt ttttcgcttc ttccgcatcg ccccaggatt cgactttggt
    15421 cgtaccggct tttttcaggt ctttgtagtg gttgaagtgg aactcgattt gtttgatgag
    15481 ctgctgcggc aaatcggaca aagttttgta ggcgttgccg ttgttgcggt cgtcggcggg
    15541 aacgcagacg attttgtcgt ccacttcgcc gtcgtcaacg aatttcatca cgccgataac
    15601 gcgcgcttcc aagaatacgc cggttgccaa aggttgttcg gtaacgagca gcacgtccaa
    15661 ttcgtcgccg tcttcgtcca aagtttgggg aatgaagccg tagttggtcg gtttggcgaa
    15721 gatggcgggt tcgacgcggt cgagttggaa tgcggccagt ttgcggttcc actcgatttt
    15781 gtggttgctg ccggcgggga tttcgttgac gacgttgatg atgccgccgt ccacatcgcc
    15841 gggggtcagg atttggttga agtctgccat ttggtttcct ttgtttggga aagtttgaag
    15901 tttgaaagta tagcacaaac gtccggctga aaatgcaccc gatgccgtct gaaagggtgt
    15961 acgggcgcgt gttaccgttt gcccaaaaac ctgcccagtt ccagaatcgc gcgcctgttg
    16021 gacggggaaa acactttttc cgccgcttct tccaagccga accagccgta ggagacgtgt
    16081 tcctcgggtt gcaggacgac gggcgtatcg cgcgggattt cggcagagaa gaggtgttcg
    16141 cggttttcaa acacgccttt tggatagcgg tgccgccagt ggtggtagat ttcgtaaacc
    16201 gtgctgtcgt gccagtcttg aagctgcccg tccgccagca ggatgccggt ttcttcccaa
    16261 acttcgcgcc ttgccgtttg ggcgacggtt tcgcccggtt cgaggctgcc ggttaccgac
    16321 tgccaaaatc ctttcggatg cgtgcgttcg atgagcagga tgccgccgtc cccgctataa
    16381 aggacgacca gtgcggaaac ggggtatttg agcggttttg ccatcggcat ctttcggcgg
    16441 gctgcggtaa tgaaggggcg gattatagca aacgccgcac gttatggcgt ttatcctttt
    16501 ccgtatcctt ttttctgcac ggatgggacg cgccggtgtt tgccggtaaa ttttccgttg
    16561 tgtcaaaaag ataagggcgg ttgtgatttt aatgcttgcc aaagcgtcgg gcggaaacta
    16621 taatccgaaa cttatcgagt cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg
    16681 caaggggtcg aaggttcgaa tcctttcact ccgaccaaaa attccgaaag ccgctttcaa
    16741 aagcggcttt tttgccgtcc gtatgattat gatgtagagt acgcggcgac agacattcaa
    16801 atgccgtccg aaaaccgttc agatggcatc tctttatctt
//
LOCUS       DAWKOD010000028        16661 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000028 DAWKOD010000000
VERSION     DAWKOD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.028"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(49..1218)
                     /locus_tag="WJK73_000712"
     CDS             complement(49..1218)
                     /locus_tag="WJK73_000712"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ7557229.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1401..1511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1564..2460
                     /locus_tag="WJK73_000713"
     CDS             1564..2460
                     /locus_tag="WJK73_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ7557230.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WJK73_000714"
     CDS             complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WJK73_000714"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ7557231.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJK73_000715"
     CDS             complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJK73_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ7557232.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4865..6817)
                     /locus_tag="WJK73_000716"
     CDS             complement(4865..6817)
                     /locus_tag="WJK73_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ7557233.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVNIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7094..7717)
                     /locus_tag="WJK73_000717"
     CDS             complement(7094..7717)
                     /locus_tag="WJK73_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ7557234.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7923..8552
                     /locus_tag="WJK73_000718"
     CDS             7923..8552
                     /locus_tag="WJK73_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ7557235.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8673..11069)
                     /locus_tag="WJK73_000719"
     CDS             complement(8673..11069)
                     /locus_tag="WJK73_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ7557236.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPKVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11221..12336
                     /locus_tag="WJK73_000720"
     CDS             11221..12336
                     /locus_tag="WJK73_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ7557237.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQHTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12339..12938
                     /locus_tag="WJK73_000721"
     CDS             12339..12938
                     /locus_tag="WJK73_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ7557238.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12939..13586
                     /locus_tag="WJK73_000722"
     CDS             12939..13586
                     /locus_tag="WJK73_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ7557239.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13604..14149
                     /locus_tag="WJK73_000723"
     CDS             13604..14149
                     /locus_tag="WJK73_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ7557240.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14168..16429
                     /locus_tag="WJK73_000724"
     CDS             14168..16429
                     /locus_tag="WJK73_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ7557241.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQTNIDFRKDG
                     KNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLK
                     RLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISL
                     DFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
                     QGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNAD
                     TTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVE
                     AADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANS
                     ISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
                     GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLN
                     TQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFIT
                     PRIMGTAGNSLRY"
BASE COUNT         4488 a         4735 c         3791 g         3647 t
ORIGIN      
        1 aatgccgtct gaactgttca gacggcattt ttgattttca aaccggaatt acagccccgc
       61 tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa gtgaactcag gttcttcgcg
      121 gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg cgcaagagat cgagcatttg
      181 gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact aaggtaatca gtttttcctc
      241 gctgattttg cctgtgccga aagtgtcgat ggaaatcgaa gtcggttcgg caacgccgat
      301 ggcgtaggaa acttggattt ggcattgggt tgccaaaccg gcggcgacga tgtttttcgc
      361 aacgtagcgg caggcgtaag cggcggaacg atccactttg gacgggtctt tgccggagaa
      421 cgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatca acgatgattt tacggccggt
      481 caaaccgcag tcgccttgcg ggccgccgat gacgaagcgg ccggtcgggt tgatcaggta
      541 tttggtttcg tcggtcagca gttcagacgg caggacgggc ttgataatct gctcaatgac
      601 ggcgtttttc aactcttcat aaccgatttc cgggtcgtgt tgcgtagaga ggacgacggt
      661 atcaatgcgt tttactttgc cggattcgct atcgtaaacc acggtcagtt gggctttggc
      721 atcaggacgc agccacggca ggcggccgtc tttgcgcaat tcgctttggc gttgcatcag
      781 gcggtggctg taatagatgg caaacggcat cagggtcggg gtttcgtcgc aggcgtagcc
      841 gaacatcaga ccttggtcgc ctgcgccttg gttcaagtcg ataccttcgc cttcgtttac
      901 gccttgggcg atgtcggggg attgttggtc gtagtacacg ccgaccgcgc agccgttggc
      961 atcaaagccc agctcggagg agttgtagcc gatgcgtttg atggtttcgc gtgcgacttt
     1021 gatgtagtcc acttgggcgg tggtggtgat ttcgcctgcc aatacgcaca agcctgtgtt
     1081 gaccaaagtt tctgcggcga cacgcgcttt ggggtcttgc gccaagatgg catccaaaat
     1141 cgcgtcggat acttggtcgg caactttgtc cggatggcct tcggataccg attcggaagt
     1201 aaacagatat tcgctcattg agattttcct tgaaaaacaa accatcttct tcagacggca
     1261 tgttgtatga acataatgtc gacagcggga aatatagcaa aatttcccta ttcataccat
     1321 tcagttgaga aatattccca tttgaatggc actttggaat ctctgcccgt acgtttctta
     1381 caggcaaaaa aattcccgca tcaagcgggt ttggattgct ctggtgagcc acatcggctt
     1441 ttcaaccgtc caccttactt ttccttttga aaagcaggtt ggcatggaat tcccaactct
     1501 taatgcagcg cggatggtag cagaaaagcc tttccgccgc aatacttccc tttttcagac
     1561 ggcatgtttc gtttacaatt cgggctgttt ccccctttgc gaaccgccat gcacatcctg
     1621 ttgaccgccc tgctcaaatg cctctccctg ctgccacttt cctgtctgca cacgctggga
     1681 aaccggctcg gacatctggc gttttacctt ttaaaggaag accgcgcgcg catcgtcgcc
     1741 aatatgcgtc aggcaggcat gaatcccgac cccaaaacag tcaaagccgt ttttgcggaa
     1801 acggcaaaag gcggtttgga acttgccccc gcgtttttca gaaaaccgga agacatagaa
     1861 acaatgttca aagcggtaca cggctgggaa catgtgcagc aggctttgga caaacacgaa
     1921 gggctgctat tcatcacgcc gcacatcggc agctacgatt tgggcggacg ctacatcagc
     1981 cagcagcttc cgttcccgct gaccgccatg tacaaaccgc cgaaaatcaa agcgatagac
     2041 aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa ccgcgcctac cagcatacaa
     2101 ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag caaccatcgt cctgcccgac
     2161 cacgtcccct cccctcaaga aggcggggaa ggcgtatggg tggatttctt cggcaaacct
     2221 gcctatacca tgacgctggc ggcaaaattg gcacacgtca aaggcgtgaa aaccctgttt
     2281 ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt tgcacatccg ccccgtccaa
     2341 ggggaattga acggcgacaa agcccacgat gccgccgtgt tcaaccgcaa tgccgaatat
     2401 tggatacgcc gttttccgac gcagtatctg tttatgtaca accgctacaa aatgccgtaa
     2461 cgaaaataaa aatgccgtct gaacaatttc agacggcatt ttgtcatttg acgatttccg
     2521 acagcggcca gcgcgggcgt acattaaatg caccggcttc cctacccgtt ttcaaatgca
     2581 tcgcgcctgc aaaggcaatc atggcaccgt tgtccgtgca gtatgccgtc ggcgggaaaa
     2641 acacgctgac tttttcggac ggatgtttcg gcttgcctgt tggggtcggg atttgcaccg
     2701 tcatgctgcc gaaagtttca cggagtttgc ggtttgcacc gaccccgccg gcgaccacta
     2761 cggttctgaa ccctgtctgc aacagggctt ttttcacttt cgccgccaac acatcgacca
     2821 ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg ctcaggaatg tcatccgccc
     2881 cgttttccgc gcgcactttc tcaacggcgg tcaatacggc ggttttcaaa cctgaaaaac
     2941 tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa aacaaacgct tcgggcctgc
     3001 ccgattccgc aagttccgac agtttcgcac cgcccggata cggcaagccc agcagtttcg
     3061 ccgttttgtc gaatgcctcg cccgccgcat catcgacgct ctcgcccaaa agcgcgtagt
     3121 cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc cgaaaccagc agcgcgacaa
     3181 aaggaaagtc gggtttttcc tccgccaaca gcggcgacag cagatgtcct tccaaatgat
     3241 ggacgggaat aacaggcttg tccaacgcta aagccagcgc gttggcgtag ctcgaacccg
     3301 ccagcagcgc accgcccaaa ccgggcccct gcgtaaaggc aaccgcgtca atgtcgccat
     3361 acgatgcgcc cgcctgcgcc aaacagcctt cagtcaacgg aacaaggcgg cggatatggt
     3421 cgcggcttgc caattccggc acaaccccgc cgtattcggc gtgcattgcc atttgagtgt
     3481 gcaggcagtg cgcccgcaat ccacgttccg tatcgtaaag cgcaacgccg gtttcgtcgc
     3541 aagaagactc gattcctaat accaacatgg tctgatgccg ttaaaaactg aaaaacgtat
     3601 tttagcggat ttcggcacga ctgccgtatc ccaaaaacgg aacatgccgt ctgaagaccg
     3661 ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa gaatgcagcc cgctcaaaaa
     3721 catattcacg ccgataaagg caaatgcggt tacgaacaaa ccgataatcg cccaccacgc
     3781 cagcactttg ccgcgccaac cggcaaccag ccgcaagtgc agccaaacgg cgtaattgag
     3841 ccagacgatg aacgcccacg tctctttcgg atcccaactc caatagcgtc cccaagcatc
     3901 tgccgcccac agcgcaccca aaatggtggc aatggtaaag aacagaaagc cgacggcaat
     3961 cgccttatac atcacctcgt cgatcaatgc cgacggcggc agccacagtt ttccgccttt
     4021 tccttccgca cgcagggaaa ccagttcggc aataccgagc atcgcggaaa tgcaaaacgc
     4081 gccgtaaccg ataaagtttg ccggaacgtg gattttcatc caccaagatt gcaatgccgg
     4141 aatcagcggc tggatggtat gcgcctcgcg ggacacgctg taccacaaga caaatccaac
     4201 cacgaccgcc ataaagccga acacgaagcc gcccaatttc tgtatggcga acttaccttc
     4261 ataataaaga tacatcagcg cggtaatcac caaaaacagg atgaacactt catacaggtt
     4321 ggaaaccgga atatgccccg catcgggacg gagcagatag ctttcgtgcc aacgtaccag
     4381 cagaccggta aagcctgcta cggcagacac ccatgcaaac acggttccca tacccaacag
     4441 cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg cccgaaatat aggcgaacag
     4501 ggcgaagaag acaaaggcgc actgccacat gatcgccgac tggctgctga ggaaataccg
     4561 caacaggaaa atctctgccg atttgatgtt gccgccatac aaaccgacgg cggcataggc
     4621 aagcaatacg cttaaaggaa caaaccagcg catcggtttg aaaaaccaac ccaaaaacac
     4681 ggcaataccg gcacttgccc acaacatgac cgtttcgtaa atgtccatat gcataccgga
     4741 acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg gcaaataccc aatcccaaag
     4801 attcagattg ctgatcaaag acttctgaat cagcagctcg tgttccggaa gggttttata
     4861 gtgttcagtc atgattcaag tccttgccga gccgttgcag actctcgacg tgttttggaa
     4921 attccttctg caaatcccgt tcgctgcggg ccgaagacat ggcaaaacgg attttgccgt
     4981 ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa catcaatacc gtacccaata
     5041 ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg ggaacgggtc atctgcaaac
     5101 ccgacgaacg cacctcggaa aacccatcaa gttgcagcag cataggcgcg ggatattcgg
     5161 tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg attccgcgct tcatcctgct
     5221 gccattcggg caagccgtac cggcgtatgg tttcatccaa agcagcgttc atcacgccgt
     5281 aaagcatttc gtagaaatag ccctgcatct tatcctgctg ctctttcggg atattggacg
     5341 taataaattc gtccaatccc aaatagcctt tttgtgcaaa gatgttcagc gtgttttccg
     5401 cagccagcat gaattgttcg cggatttcgg caggtgcgcc tttggttgcg tcggcaacca
     5461 gacgtttgcg cccttcccca tctttcaaaa actcacgcaa tgccataaag gtgtccgctt
     5521 tcaactgctt gtccaagggg atacgcagcc agcggtattg ctgctgcaag ccgctgcgcg
     5581 tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg cagcatatag tttttatatt
     5641 cgaccgcctg ccctgccgca tcacggatac ggtaaacaat ggaagggccg atattggtgt
     5701 attttttacc ttcctgagta acggcgcgga catcgttcag cgtggatttc aggctttttt
     5761 cccgttccgc gccctcgctc atgtcctcca cattcataga agtaaactga tcgaactcaa
     5821 gacgatattt gtgtttgcca atttccaacg gaaactggtg tatggatgtt gccttcaaca
     5881 cgacaggctc gcgcgaagca tcacccaaat tccacgcctt gaatgtcaaa tccgaaccgc
     5941 cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa ggtcaaagga tggttcacgc
     6001 ggatggtgcg ctcgagtttc tcaccggttg ccttatccgt tacttcaata tcactggcaa
     6061 aatcgcgcgg catacccgta ttgtaaaaat cgatatggaa ttttttcagt ttgacttcaa
     6121 aaggcaagtc ctgaaccaat atcccgttgt cggcattcag gaaaaccaca tccgcactct
     6181 gcccctcgga aatattgacg ttgcccctaa atgagagatt ggacgcaccc aaaatacttt
     6241 cgggcttgaa atccttggca taaaccgcct gattgtccgg aacaatccga ccggtcagca
     6301 tacccagttt caacagcagg ttactgtcta tcaacccgcc caggcaaatg acaatcaaag
     6361 caacatgggc aaagatatag ccccatttgt tcattgtgcc ttttttggcg gcaatcagaa
     6421 ccgacccgtc ttcacggtta atggtttttc cctgaaaacc ttgtacttcc agataacgtt
     6481 tggcaacctc gggcgcaatg tttacatcca acagcgaaga atggcgcatc gccgccagag
     6541 atttttcttt aaccttttcc cgaaaagact tcatttcgcg ccagaacggc ggcacattgc
     6601 gaatcaggca caaactggta gaaaccacca aaaacatcat gataacgaca aaccatgccg
     6661 aagcatagac gtcatacagt cccagaaaac caaaaatctg cgcccaaaac gatccgaatt
     6721 tgaccaaata atccgtctgc ggctggtttt gctgcaacac cgtaccgata accgatgcaa
     6781 tacccagcag actgagcaaa gcgactgcaa agcgcatgga gctgaaaaaa gcgaaccacg
     6841 gacgggaaag aagtggggga gatctacggg atttactcat tgtgtgttta ttccgccatc
     6901 aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac gtcccgattc tactgtcttg
     6961 atgcttttta tttcaagaca atgaagacag cctgcatcga ttccaacggt tgcgattgaa
     7021 aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt ttcagacggc ctctaaaaca
     7081 gactattgcg gaattaacgc aaaccttgga taaagttggc gaccgctttc aaatcttctt
     7141 cagacatacg gtttgcaata tcttccatga tggtattttt acgctgaccg gacttgtagg
     7201 cattcatctg ttcaacaata tatgcctgat gctgaccgcc caaacgcgga taagcctgaa
     7261 tttcgcttcc gcctcccggc atacccgcac cgctcggacc gtggcaggac atacacgccg
     7321 gcactttttt atcgctcaaa ccgccgcgat agattttcgc acccaattcg ggattttcct
     7381 taggattggc ttcaccggat ttgggctgct gtttggcata gaatgcggat acgttcaaaa
     7441 tatcctgatc gctcaaattc attaccaccg gtttcatcac agctgccgaa ccgtgggtgc
     7501 gtttaccgtc gcggatgccg atggtttgat gatagatgta agcagtatgc tgtgccgcca
     7561 aacgcggata catcgcaatg ccgctgttac cgtctgctgc atggcaagcc gcacaaaccg
     7621 ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg agaggcggaa accgcaccgg
     7681 cagccaaaac aaaggccaat aaagtcaatc gtttcatgga gtgctcctga ttacagcatt
     7741 ggataacgca acaatgctct ttttatattc aaatacggga tttttgaccc gattaaaacc
     7801 gatgattctg taaacgtgtt attctatact aaatttacat taaattacca ctgtgtttca
     7861 cataaaacca accgcatatt tttgctgtcg gacaaacggc ggcggaaaac aaggatatgc
     7921 ccatgaacct ttttcaaaac gccaaattct tcacgacggt caaccacctt aaagacctgc
     7981 ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa tgccggaaaa tccagtgcca
     8041 tcaataccct gaccaaccat gtccgtcttg cctacgtttc aaaaacaccc ggacggacgc
     8101 agcatatcaa cttcttcgag ctgcagaacg gcaattttat ggtcgatttg cccggctacg
     8161 gttatgccca agtccctgaa gcagtacgcg cacattgggt caatctgctc ggcgactatc
     8221 tgcaacagcg caaacagctt atcgggctgg ttttgattat ggatgcccgc catcctttaa
     8281 aagaactcga catccgtatg ctggattttt tccacacgac cggcagaccg gttcacatcc
     8341 tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat aaaaaccctg tcccaagtca
     8401 aaaaactgct caaaccttat tccgacaggc aaaacatcag cgtacagctg ttttccagcc
     8461 tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg aagctggttt gacgcagcag
     8521 atgccgccgc ttcctctcca gaggaaaact gaccccaatt atacggaaac cgtatttccc
     8581 ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc caaatctaaa atgccgtctg
     8641 aaagactttt cagacggcat tttgcggagt ctttaaaaca gagaatccaa ctgctgctgt
     8701 ttggaaccag tattactcgg aagcaccggc gtttcctgca tatcttggcg gacttcgtca
     8761 tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat cttcttttgc ccgtcgggaa
     8821 ggttgcggcg caataccgct gttgtccagc gtcaagcccg gatcggttac catacgttcc
     8881 ttcatatagt attcgccatt gctgctgacc acaccttcag gcattttcat ccccttgccc
     8941 tgctttcctt tcaacgcaaa acgcatatag tccacccaaa ccggcaccgc aatcgtaccg
     9001 ccgtagccga cacgccccat actcttaggt ttgtcgaagc cgatatatac ggcagtaacc
     9061 acatcagggt taaaaccgac aaaccacgca tccttattgt cattggtcgt acccgtttta
     9121 ccggcaatat ccgttcttcc caacgcagct gccccccttg ccgtaccaac acggaccaca
     9181 tcctgcataa tcttatacat aatataggca ttgcgcggat cgattgcctg aggcgcattt
     9241 tgcccggcca ccaaaggttg catttgggcg cgcaacctgc cgtctctgtc ataaatctta
     9301 tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa atacgctata tgcctccgcc
     9361 actttcaacg gcgttgtctc gcccgtacct aaagccatag acaggcttgc cggcagctcg
     9421 gacgacctga agccgaaacg ccggatatac tgttgcgcgt aaccgacacc gatagacatc
     9481 aaaatacgga tggaaaccat attcttggaa gccgtcagag cctgtctcaa agtaatgtag
     9541 ccggaatatc tgccgtctga atttttaggt gtccaaaccg aaccgttcgg ccctttcccc
     9601 ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg tcatcccctt agataatgcc
     9661 gccgaataga caaacggctt aaaggtcgaa cccggctgcc gcattgcctg aacggcacga
     9721 ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg cgcgcacagc tccggttttt
     9781 gcatccagcg aaaccaaagc cccctgcagc aacggctctt gaaccaccgc ccaacgcccg
     9841 ccgttgtttt tgacacggat gaccgcgccc ctgcggatac ggtcctcccc cattttttca
     9901 ttattgaccg cgcgggccgc aaaacccaag gcgcgcctgt caagcgtaac ccgcctgccg
     9961 ccgggcagct gtatgacgac atttttcttt ttagtcacat ccaacacaac ggcgggaacc
    10021 attttatcga cggtatagag tcccgacaga tactggctga cagtctcctc gacatcttca
    10081 ctcttactca aatcgatata gttttccgca ccgcggtagc tgctgccgcg atcgaaattc
    10141 cgtagagcct tgcgcaatgc ctcggttgcc accttctgat gatcggcgcg gaccgtggta
    10201 taaaccttaa aaccctgcgt ataggcatct tcaccgtatt tctcatacag ttcctgacgc
    10261 accatttccg ccacatataa agcactctga tcgattttcc gaacaaaccg ctcgtaatgc
    10321 agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa tcatcttctc ctcgagcata
    10381 ttgttcaaaa tatacttctg gcgcaacttg gcacgttctg gattaacaat cggattatag
    10441 gcagacggag ccttgggcag tcccgcaagc atggcggctt ccgccaaagt caaatctcgg
    10501 acattcttat tgaaatagat ttgcgcggca gatgcaaaac cataggcgcg ctgaccgagg
    10561 taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc ttaaagactg ctcgatttta
    10621 taggcaagca acacctcatt gaatttgcgt gtgaacgttt tttcactgct caaataaaaa
    10681 tttttcgcca cctgctgcgt aatcgtactc gcacccgact gcacgctgcc ggacacgaca
    10741 ttgccgacgg cagcgcgggc aacaccccaa acatccaccc cccaatgccg gtaaaagcgt
    10801 ttatcctcgg cggcgataac cgcattccgc aacacctttg ggaaatcgcc gatttttgta
    10861 aattcgcgcc gctgctcccc atacataccg atgacttccc catccgccga ataaatagtc
    10921 aacggcattt taggctggta atgctgcaaa gaatccaaag acggtagttt cggatacgtt
    10981 accaaaatag caatggcaac caaacccact ccaaatacac aaaacccaaa aaccaaacca
    11041 aaacaagtcg ttaaaatctt tttaatcata gctgaataat aatttaccat tattggtatt
    11101 aaataaagta aaatagcaac cgatttctac aaagcacggt ttcaatgtgc aaagaacaag
    11161 gaatccatta cggataccga aacggttact cactgtacaa ataaagcagg aactttcatc
    11221 atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag atgccaagct ccctaaaaaa
    11281 tcttcgggac tcaataaccg cgcggcaatc ggcatcgata tcgaccagca ttccatcaaa
    11341 atggtccaat tgtcaggacg tagtttaaac caaattcaat tggaaaaata cgtcattgcc
    11401 aaattaccaa agaatatcat tcaaggcaat aaagtccaaa attacgatca acttgttaca
    11461 tatttgcaac aagcctatgc caaactgggt acttcgtgca aaaacatcat cgcgtccgtc
    11521 ccgcaaaatc tggcaaccat cgaacaattg acctacacag acaaagatgc agaattagac
    11581 ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct cgatatcgct cgaagaagcc
    11641 aattacgact atcaggtctt gtcccaatcg gccgccggcg aagctgtgtt ggccgtcgca
    11701 tcgagaaagg atgaaatcga acccctgatt gacgcattca acgccgccgg tatgaaatta
    11761 tccgcgcttg atgtggacat tttcggacaa tacaacgcct acgcgctatg gataaaccat
    11821 ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg gcgtatatgc cgcacagacc
    11881 tacgccttgg tcatccaaga cggaaaaatc ctatacaaac aggaaacctc cgtcagcgaa
    11941 gaacagctca accaactcat ccagcacacc tatcaggtaa cagaagaaaa agcggaagaa
    12001 atcatcaact ccccgcaaaa accttccgat taccaagaaa gcgtggcaaa ctatttcaac
    12061 cagcagatta cccaagaaat acaaagggtc ttgcagtttt attacaccac gcagaccgca
    12121 gacgatatga ccgacatcaa gcatatcctg ctgaccgggg aagcggcgcg ccaggaaggc
    12181 atcgcccaaa ccgtcgcctc acaaaccaat gcagatgtac aatgcgtcca tcccgcgcgt
    12241 tattttgcgg acaacctcaa aacagacaaa caacaattcg aacttgatgc gccgacactg
    12301 accagggcgt tcggtttggc ggtacgggga ttataattat gaacaattta atcaaaatca
    12361 acctcctccc ctacagggaa gagatgaaca agcgcaaaca gcagcagttt aaaacgctga
    12421 tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc aacctacctg tttatcgaca
    12481 atatgatcaa taaccagtcg gaaagaaaca cgctgctgga aacctccatc gcacacttgg
    12541 ataccgagct gtcggaaata caaaagctca aacaggaaaa agatgccttc ctgattaaga
    12601 aaaacaaaat cgaggagctc cagctcaaac gcctccaagc cgcaaaaatc ctcgacagcc
    12661 tgaatgaggc cgtccccgga agcacctacc tgacctcgct ggatgccgtt accgccgact
    12721 cttatcggct cagcggcagg acatccagcg acaaccgcgt tgccgccatg atgagggcga
    12781 tgcccaatac cggcatattc aagcaacccg aattgttaag catcaagaaa aacaattcgc
    12841 atcaagaatt tacccttcag gcaacattac aacccatcgt aaaggcggcc gaatccaaag
    12901 agaatccggc ttcgggaaac gcacaggagg caaactgaat ggcttctaaa tcatctaaaa
    12961 ccaacttgga tctcaacaac cttcacctgc tcaaccttcc tgccaggctt tttatcgccc
    13021 tgctggccgt tgccgccgtg ctggggctcg gttatgccgg attgttcaaa agccagatgg
    13081 aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa aaacacctac aaacagaaaa
    13141 gtatcgacgc ggccagcctg aacaacctga gggacgaact tgcctcaatc cgctctgcct
    13201 tcgatatcat gttgaaacag ctgccgacag atgcagaaat tcccaacctg gttcaagagc
    13261 ttcatcaggc aggttcgagc aacggtctgc gcttggacag cgttatgccc caacctcccg
    13321 tagatgacgg ccccatcaaa agattaccct attccatttc cattaccgga aattacgaac
    13381 agatcagcca atttacccgc gatgtcggca gcctctcccg aatcattacc cttgagtcgc
    13441 tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga cggcaagagc agcatcctga
    13501 acctcagcgc cattgccacc acctaccaag caaaatccgt agaagagctt gccgcagaag
    13561 cggcacaaaa tgccgagcaa aaataactta cgttagggaa accatgaaac actatgcctt
    13621 actcatcagc tttctggctc tctccgcgtg ttcccaaggt tctgaggacc taaacgaatg
    13681 gatggcacaa acgcgacgcg aagccaaagc agaaatcata cctttccaag cacctaccct
    13741 gccggttgcg ccggtataca gcccgccgca gcttacaggg ccgaacgcat tcgacttccg
    13801 ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac accaagcgta ttaaagaaac
    13861 gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc attttgaagt ccggacagaa
    13921 agtctccggc ttcatcgagg ctgaaggtta tgtctacact gtcggtgtcg gcaactattt
    13981 gggacaaaac tacggtagaa tcgaaagcat taccgacgac agcatcgtcc tgaacgagct
    14041 aatagaagac agcacgggca actgggtttc ccgtaaagca gaactgctgt tgaattcttc
    14101 cgacaaaaac accgaacaag cggcagcacc tgccgcagaa caaaattaag aagaggatta
    14161 ctccattatg aataccaaac tgacaaaaat catttccggt ctctttgtcg caaccgccgc
    14221 ctttcagaca gcatcggcag gaaacattac agacatcaaa gtttcctccc tgcccaacaa
    14281 acagaaaatc gtcaaagtca gctttgacaa agagattgtc aacccgaccg gcttcgtaac
    14341 ctcctcaccg gcccgcatcg ccttggactt tgaacaaacc ggcatttcca tggatcaaca
    14401 ggtactcgaa tatgccgatc ctctgttgag caaaatcagt gccgcacaaa acagcagccg
    14461 tgcgcgtctg gttctgaatc tgaacaaacc gggccaatac aataccgaag tacgcgggaa
    14521 caaagtttgg atattcatta acgaatcgga cgataccgtg tccgcccccg cacgccccgc
    14581 cgtaaaagcc gcgcctgccg caccggcaaa acaacaggct gccgcaccgt ctaccaagtc
    14641 cgcagtatcc gtatccgaac cctttacccc ggcaaaacaa caggctgccg caccgtttac
    14701 cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg cggcggcatc
    14761 agcaaaacaa caggcggcag caccagcaaa acaaaccaat atcgatttcc gcaaagacgg
    14821 caaaaatgcc ggcattatcg aattggctgc attgggcttt gccgggcagc ccgacatcag
    14881 ccaacagcac gaccacatca tcgttacgct gaaaaaccat accctgccga ccacgctcca
    14941 acgcagtttg gatgtggcag actttaaaac accggttcaa aaggttacgc tgaaacgcct
    15001 caataacgac acccagctga ttatcacaac agccggcaac tgggaactcg tcaacaaatc
    15061 cgccgcgccc ggatacttta ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg
    15121 cggcgtgaac aatgcgccca aaaccttcac aggccggaaa atctcccttg acttccaaga
    15181 tgtcgaaatc cgcaccatcc tgcagatttt ggcaaaagaa tccggaatga acattgttgc
    15241 cagcgactcc gtcaacggca aaatgaccct ctccctcaag gatgtgcctt gggatcaggc
    15301 tttggatttg gttatgcagg cgcgcaacct cgatatgcgc cagcaaggga atatcgtcaa
    15361 catcgcgccc cgcgacgagc tgcttgccaa agacaaagcc ctcttacagg cagaaaaaga
    15421 cattgccgat ttgggtgcgc tgtattccca aaacttccag ttgaaataca aaaatgtgga
    15481 agaattccgc agcatcctgc gtttggacaa tgccgacacg accggaaacc gcaacacgct
    15541 tatcagcggc aggggcagcg tgctgatcga tcccgccacc aacaccctga ttgttaccga
    15601 cacccgcagc gtcatcgaaa aattccgcaa actgattgac gaattggacg tacccgcgca
    15661 acaagtgatg attgaggcgc gtatcgtcga agcggcagac ggcttctcgc gcgatttggg
    15721 cgttaaattc ggcgcgacag gcaagaaaaa gctgaaaaat gatacaagcg cattcggctg
    15781 gggggtaaac tccggcttcg gcggcgacga taaatggggg gccgaaacca aaatcaacct
    15841 gccgattacc gctgccgcaa acagcatttc gctggtgcgc gcgatttcct ccggtgcctt
    15901 gaatttggaa ttgtccgcat ccgaatcgct ttcaaaaacc aaaacgcttg ccaatccgcg
    15961 cgtgctgacc caaaaccgca aagaggccaa aatcgaatcc ggttacgaaa ttcctttcac
    16021 cgtaacctca atcgcgaacg gcggcagcag cacgaacacg gaactcaaaa aagccgtctt
    16081 ggggctgacc gttacgccga acatcacgcc cgacggccaa atcattatga ccgtcaaaat
    16141 caacaaggac tcgcctgcgc aatgtgcctc cggtaatcag acgatcctgt gtatttcgac
    16201 caaaaacctg aatacgcagg ctatggttga aaacggcggc acattgattg tcggcggtat
    16261 ttatgaagaa gacaacggca atacgctgac caaagtcccc ctgttgggcg acatccccgt
    16321 tatcggcaac ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt
    16381 cattaccccg aggattatgg gtacggccgg caacagcctg cgctattgat gcgtcaaaat
    16441 aagggcatat gttttacggc atatgccctt tctttatgct ttttgccgcg accgaaatgc
    16501 cgtcattccc gcgcaggcag aaatccaact tgtccggttc cggttgtttt tcgtctcgta
    16561 acttttgagc cgtcattccc gcgcaggcag aaatccagtc cgttcagttt cggtcatttc
    16621 cgataaattc ctgttgcttt tcatttctgg attcccactt t
//
LOCUS       DAWKOD010000029        16581 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000029 DAWKOD010000000
VERSION     DAWKOD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.029"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..473)
                     /gene="rfbC"
                     /locus_tag="WJK73_000725"
     CDS             complement(<1..473)
                     /gene="rfbC"
                     /locus_tag="WJK73_000725"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7557242.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIDWPL"
     gene            complement(527..1393)
                     /gene="rfbA"
                     /locus_tag="WJK73_000726"
     CDS             complement(527..1393)
                     /gene="rfbA"
                     /locus_tag="WJK73_000726"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7557243.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            complement(1499..2539)
                     /gene="rfbB"
                     /locus_tag="WJK73_000727"
     CDS             complement(1499..2539)
                     /gene="rfbB"
                     /locus_tag="WJK73_000727"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ7557244.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            complement(2540..3583)
                     /gene="galE"
                     /locus_tag="WJK73_000728"
                     /pseudo
     CDS             complement(2540..3583)
                     /gene="galE"
                     /locus_tag="WJK73_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(3576..4578)
                     /gene="dcm"
                     /locus_tag="WJK73_000729"
                     /pseudo
     CDS             complement(3576..4578)
                     /gene="dcm"
                     /locus_tag="WJK73_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(4609..6882)
                     /locus_tag="WJK73_000730"
     CDS             complement(4609..6882)
                     /locus_tag="WJK73_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ7557245.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKVSDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(6946..7596)
                     /locus_tag="WJK73_000731"
     CDS             complement(6946..7596)
                     /locus_tag="WJK73_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557246.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(7593..8390)
                     /locus_tag="WJK73_000732"
     CDS             complement(7593..8390)
                     /locus_tag="WJK73_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7557247.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(8390..9553)
                     /locus_tag="WJK73_000733"
     CDS             complement(8390..9553)
                     /locus_tag="WJK73_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ7557248.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(9568..10731)
                     /gene="ctrA"
                     /locus_tag="WJK73_000734"
     CDS             complement(9568..10731)
                     /gene="ctrA"
                     /locus_tag="WJK73_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ7557249.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            10950..12068
                     /gene="wecB"
                     /locus_tag="WJK73_000735"
     CDS             10950..12068
                     /gene="wecB"
                     /locus_tag="WJK73_000735"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ7557250.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            12070..13707
                     /locus_tag="WJK73_000736"
     CDS             12070..13707
                     /locus_tag="WJK73_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ7557251.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            13780..14523
                     /locus_tag="WJK73_000737"
     CDS             13780..14523
                     /locus_tag="WJK73_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7557252.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            14525..15388
                     /locus_tag="WJK73_000738"
     CDS             14525..15388
                     /locus_tag="WJK73_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7557253.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            15433..16452
                     /gene="galE"
                     /locus_tag="WJK73_000739"
     CDS             15433..16452
                     /gene="galE"
                     /locus_tag="WJK73_000739"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7557254.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNNP
                     NGYDD"
BASE COUNT         4703 a         3800 c         3526 g         4552 t
ORIGIN      
        1 tgaagcggcc agtcgatgcc gactgtcggg tcgttccata ttaaaacctg ttcggcttca
       61 ggcttgtaat agtcggtgca tttgtacacg acttccgccg cgtcgcccaa aacgcaaaac
      121 ccgtgcgcga agccttcggg tatccaaagc tggcattggt tttgcgcgga cagggttgcg
      181 cccgcccatt tgccgaaagt cggcgaacct tcgcgcatat cgacggccac gtcgaacact
      241 tcgccgacaa ctacgcgtac gagtttgcct tgtgtgtttt cggtttggta gtgcaggccg
      301 cgcaatacgc ctttgccgga tttggagtgg ttttcctgca cgaaggtgcg ttcgcagact
      361 tgggttttaa accactcgtc gcggaaggtt tccataaaaa agccgcgcgc gtcgccgaag
      421 acttggggct caagcagttt tacgtcagga atggcggtat caatgatgtt catcttttta
      481 tctttcatct aaaggccgtc tgaaaagttt cagacggcct caaacattat tttttcaaca
      541 ggcgcagcaa atattggccg tattggtttt tcgccatcgg gcgcgccaat tcttccagtt
      601 tttcatcgga aagccaaccg ttgcgccaag cgatttcttc caggcaggcg atgtgcaggt
      661 tttggatgtt ttgcacggtt tggacgaacg aagccgcctc gtgcaggctt tcgtgcgtac
      721 cggtgtccag ccacgcgaaa ccgcgtccca atatttgaac ggagagcgag ccgtcttcca
      781 aatacatccg gttgaggtcg gaaatttcca attcgccgcg tgcggacggt ttgagctgtt
      841 tggcgaactc gacggcgcgg ttgtcgtaga aatacaagcc ggttaccgcc caatcggatt
      901 tgggccgttg cggtttttct tcgatggaaa cggcgcggaa gttttcgtta aattcaacca
      961 cgccgaaacg ttcggggttt ttgacctgat aagcaaacac ggttgcgccg tgcgtttgcg
     1021 ctgccgcctg tttcaatgtt tgcgtaaacg actgaccgta aaaaatattg tcgcccaaaa
     1081 ccaagcaaac attgtcgttg ccgataaatt cttcgccgat gataaatgcc tgtgccaagc
     1141 cgtccggact gggttgcacg gcataactga tggaaatgcc gaaatcgctg ccgtcgccaa
     1201 gcaggcgttt gaaagaggcg ttgtcttcag gcgcggtaat caccaaaata tcgcggattc
     1261 ccgccagcat caaaaccgac aaggggtaat aaatcatcgg tttgtcgtac acgggcagga
     1321 gctgtttgga tacgccgcgc gtgatggggt agaggcgcgt gccgctgccg cctgccagta
     1381 tgatgccttt catcttttct ttcttccttt gcgatgggtt ttcagatggc attgcgtcgg
     1441 gatgccgtct gaaaacttat tttccggtgc ctaaacgttc caaacgatag ctgccgtttt
     1501 atgcgttttg ccgccgagtt ttgttgtcca agtaccattg cactgttttg cggaggccgg
     1561 attcaaaggt ttccaaaggc tgccagccca aatcccgtct gattttcgcc gtatcgacgg
     1621 cgtagcgggc atcgtggcca gggcggtctt gtacgaaagt aatcaaatct tcataacgcg
     1681 ccacgccggc cggtttttcg ggagcgagtt cttccagcag ggcgcagatg gttttgacga
     1741 cttcaatatt ggctttttca ttgtggccgc cgatattgta ggtttcgccg acaacacctt
     1801 cggtaacaac ctgatacagt gcgcgcgcgt ggtcttcgac aaacagccag tcgcggattt
     1861 gcataccgtc gccgtacaca ggcagcggtt tgccgtcaag cgcgttcaga atcatcaaag
     1921 gaatgagttt ttccggaaaa tggtaaggac cgtagttgtt ggagcagttg gttacaatgg
     1981 tcggcaagcc gtaagtacgc aaccacgcgc ggacgaggtg gtcgctggac gctttagagg
     2041 cagagtaagg gctggacggc gcgtagggcg cggtttcggt aaacaaatcg tctgtgccgc
     2101 ttaaatcgcc ataaacttca tcggtagaaa tatggtggaa acggaaggct tcgtgctttt
     2161 cagacggcat ttgctgccgg taggcacgcg cagcttcaag taggttgaat gtgccgacga
     2221 tattggtttg gataaactcg cctgccgaac cgatagagcg gtcgacatgg ctttccgccg
     2281 ccaagtgcat cacggcatcg ggccggtgtt gtgcgaatac gcggtcgagt tcggcgcggt
     2341 cgcaaatgtc cacttgctcg aaagcatagc ggggattatc ggcaatgtcg gtcagcgatt
     2401 cgagattgcc cgcgtaggtc agtttgtcga ggttgacgac ggaatcttgg gtgtttcgga
     2461 taatatgacg gacaacggca gagccgataa agcccgcacc gccggtaacg aggatggttt
     2521 ttttgtttgc ggtttgcatt tagtccccgt atccgtcggg gttgcggctg acccaacgcc
     2581 acgaatcctc catcatttgt ttcaaaccgc gtttggtttc ccagcccgtc tcttgtttgg
     2641 tatgggacgg gtcggcaaag gaacacgcca agtctccggc gcgtcggggt tggatttgat
     2701 agggaatatt taagccggat gcggcctcaa aggcacggat gatttccaaa acggaatagg
     2761 cgcggcccga acccaagttg aacaaatgta cgccggaaag gccgcctttc gccttcattg
     2821 ccgcgatatg cccttctgcc aaatccatca catgaatgta gtcgcgcatt cccgtaccgt
     2881 ccggggtcgg ataatcgccg ccgaataccg acagttgcgg cagcctgccc gaagccactt
     2941 ggcagatata gggcaaaagg ttgttgggaa taccgttggg ctgttccccg atcaagccgc
     3001 tttcgtgcgc gccgatcggg ttgaaatagc gcaacaaaat cacgctccaa cgcgggtcgg
     3061 ctttttggat gtcggttaac atccgttcca ccatcgcttt ggatgcgcca taaggattag
     3121 cggtatcgcc cgggcgcata tcttccgtat agggcatttt acgtatcatg gcgcgacgca
     3181 ttaaagtaaa cgccaaagcg cgtttatact ggttatccag tttccctata tttttacgtt
     3241 acaaatcaag ttgttggtat tcatcgtgaa aatagaattt ttcggcataa cgctgactca
     3301 taaaaccggc atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca tttaatgttt
     3361 catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa atatcgttag
     3421 taatcacacg atagccgcgt tttttggctt cgtaggcaaa ggaacagcct ccactaaata
     3481 catctgcaac tgtatctaca tcagacggaa gctggtcaca aatctaggaa gctattttct
     3541 ctttattacc gatatagtta attttcggat attgcttatt cattttcact catttttaaa
     3601 atactttcag caatagcttt tgccaataaa ggcggtacgg cgttaccaac ctgctgctgc
     3661 tgggcaattt tgctgccgca aaaaataaaa ttatcaggga aagattgtaa ggcagctaat
     3721 tcacgaacgg ttaacgcccg attctgttca tagtgaaaaa ctttgcgcat atctcctgta
     3781 atacaaacgg ctggttttgt tgctgttgta acggatgtat ttacggatat cacctgtttt
     3841 cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa atgccatttt
     3901 ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg gattgctttc
     3961 gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat gggaaatctg
     4021 caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata tcactcggct
     4081 acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca ccgaataacc
     4141 gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt gtgtatagag
     4201 tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta ttcggacaaa
     4261 ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct ttcctgccat
     4321 actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc cgttaagaca
     4381 attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt gatgatgggg
     4441 gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa gcaattgttg
     4501 gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa ggtcaatata
     4561 ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt accgcttcag
     4621 cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc gttggggttt
     4681 gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt ttgcgccgcc
     4741 cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat cgacttccag
     4801 cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt ggacgaactt
     4861 gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt ccacgaacgc
     4921 gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt gcaagtcgct
     4981 gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg gatcacggcc
     5041 gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc gttcgtcggt
     5101 gaagtcggac actttgattt gcttcacgcg ctcgcggttg ccgatgagtt cggcggcgct
     5161 aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt ggacggcgct
     5221 cgcgtccaac ggctctttac cgccgttaat ccgcaaaaag cctgccgcct gctcgaaggt
     5281 tttttcgccc aaacgcggca ctttcagcaa ttttttgcgg ctgtcgaacg cgccgttttc
     5341 atcgcggtag gcgacgatgt tttgggcaag agtttgattc aaaccagaaa tccgcgccaa
     5401 gagcggggcg gaggcggtat tcacgtccac gccgacggcg ttcacgcagt cttcgaccac
     5461 cgcgtccagc gatttggcga gccggctttg gttcacgtcg tgctgatact ggcccacgcc
     5521 gatggatttg gggtcgattt tgaccagctc ggcaagcggg tcttgcagcc tgcgggcgat
     5581 ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgctg ccagttcgga
     5641 cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc ccattttcga
     5701 catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg tgccgttacc
     5761 gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg ccaacatatt
     5821 gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt cgtccaccac
     5881 ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca gccgtccggc
     5941 gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg cgtcggtgtc
     6001 ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga ttttcgcgcg
     6061 ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt tgaagcggcg
     6121 ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg gctgatattt
     6181 gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgctagcg cacgatggct
     6241 gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt cgccttcggt
     6301 ttctttgcct ccaacaactt gcgcgtggat ttcggcttcg ttccacagct tgtcgcgcag
     6361 cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac gcgcgccgtc
     6421 caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg cggcttccac
     6481 gtcctgcgac tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt gttcgcgcgc
     6541 gatttgcgct ttggtgcggc gtttgggttt gtagggcaga tacaggtctt ccagcgcggt
     6601 tttgttatcg gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc cttgctcttc
     6661 aatgcttttt aaaacaacgg ctttgcgctc ttccaactcg cgcagatatt gcagccgctc
     6721 ggcaagccgg cgcaactgcg tatcgtccaa cccgcccgtc gcttccttgc ggtagcgggc
     6781 gataaacggc acggtcgcgc cgtcgtccaa aagctcgacg gcggcggtga tttgcgcggc
     6841 agtcgcggag agttcttggg aaagaatttg agtaatgttc atcaataaaa ttccaacgga
     6901 caggccgtct gaaatttcag acggcctgat ttaaaaacaa tcgctttaag gcagcgaatt
     6961 ataatattcg taggctttgt ccatatcttc aaactggtac atatgccctt tttccagcac
     7021 catcgcatta tcgcaatatt gcttcatggc gctgtggctg tgcgacacca agatgatgga
     7081 acggtctttg cgcttttcaa acaactcgta cttacattta tcggcaaaac gcgagtcacc
     7141 aactgcaatc acttcgtcaa tcaaatagca atcaaactct accgccaacg acaaagcaaa
     7201 tgccaaacgc gccttcatac ctgaagaata acgcttgacc ggctcgtaaa gatattgccc
     7261 caactcggaa aactcttcgg taaagttctt cacataatcg atatcgacat tgtaaatccg
     7321 gcagatgaaa cgcaaattgt ccataccggt cagactgcct tgaaacgcac cggagaatgc
     7381 caaaggccaa gaaatactca ttgtccgctt gatttcaccc gtggtcggcg gctcaacgcc
     7441 actgatcaaa cggatgagcg tcgatttacc tgcaccgttg cggccgagaa taccgatttt
     7501 ctcgcccttc tccattttga agctaatatc gtgcaagact gtccgccaac cttggcgggt
     7561 cagatagcgt ttggaaacgt gttcaactga aatcattgcg gctcaacccc tttgctgaat
     7621 ctcgccacca ccgccaaacc aagcagcagc aaaaccaagt tacacaacaa aatataccaa
     7681 ggattttcgt aagtcgtgac gctgtcgcca aaatatccgg cacggaacat ttctgtacca
     7741 tgcaccatcg gaatcatcag cacatagtgt tgcaactgct gaggcaggtt gtgtacgaag
     7801 aaaaacacgc cggacaaagg catcatcaca aaactgatgg tactccaaac tttgccaaac
     7861 ggttcaaaat gaaacgccac cgaacagatg accagcccca atccaacggc aaacatagcc
     7921 atcaaaagcc aagccaacaa catatagaaa atatctgccg gtacatcaat ccaaccgatg
     7981 ataaccaatg cgaacataat cacgacctgc gcaatggtgg cgccggcaat ctccaacaac
     8041 atacgcgcaa aaatggtatc caatacgcgt acatttcggt gatacagcag gcttgtgttg
     8101 gcagaaatcg aaccgatagc atgattagaa gcattacgcc acatcatcat catcggatag
     8161 cctgtcagcg tgaatgccac gatattcaaa gctgacacgt tgtgcatacg gaaaaacttc
     8221 cacatcaaca ccataaccaa agtcagcagc aaaggttcga caaacaacca caaaaaacca
     8281 atgttattac gaccgtagcg agtaataatt tcacgcatta agagtgcgcc gattaccctt
     8341 ttttggataa gcagcgactc caaaaacgat gttttatgta gctctttcat cagtttttat
     8401 gctcacggat acttgcagac agcaacacga caatgccata gacaatcaaa ccgatgacaa
     8461 acgttgccac gatgttgtac aaacggtttg gctcatatgc caagtcaggt ttgttcggct
     8521 gggaaatcac ttccaaatac aattgctgac gatctgcttc cgcttttgca ctttccaaag
     8581 aagtcatcgc cgcagccaat tgtttttcgg ccaattcgtt ttccaaatac acgcgttgat
     8641 attccgccgc ctgattggac aacgaaccct cgccaccgcc ggaaatcgct ttcatttgtt
     8701 gcgagatttc tttacgcaaa ctcttctcac gcgcaatcaa acctggaatt tgcgggtttt
     8761 caggcgtaac ggcttttacc tggtccagct gggtctgaat gacaatcaac tcgtcctgta
     8821 atttggatac caaacccatt tgcacatcgg attgcgcctt caagtcgaaa ataccgttag
     8881 acacacggaa ttttgtcaat tggacagaag cctctttaac ttgctcttca gcagaattga
     8941 ccacttcctg agcataacga atcgtatcct gacgcgcacg ttcgttcagc tggttaatca
     9001 atacctcacc ctgtttaagc aaagcatcat tgatcttttg agattcaccg gcattaaacg
     9061 atgtaacgct caaattggaa atgcctgaga cagagtcaaa atggatggat accttatcac
     9121 ggtagtattg ataaaacgcc tcatcctcgc cacgcaggcc aaaaccatta aaacggctga
     9181 aaatatcgcc ttctttttca taaaaatcgc gaatgggcat tttcttacgc aacgcatcca
     9241 aagccgaacg cgactgcata tattcctgca cggtataaat atcgtcttgc gcacgggcaa
     9301 aacctgtgcc ctgcaaaatg gcacccaggc cattgagaga agattggctt ttaggcgagc
     9361 gcaccacaaa gctcgattgc gacgtaaaac gatcggaagc gaagaagccg aaatacacca
     9421 acgaaattac cgtagggata atcaccgtta cccaaaataa agggcttagc tttttaatcc
     9481 aacttttctt tttcggcttt ttacgctcgg ctttggtttc agtggcaact gccacaggaa
     9541 gttgctcaga catcttaatt actcacatta attagttaaa ttattaatac tgttcgcgcc
     9601 actggtaacc ggcgagaaca caaacgacaa gaatttctgc acttcagcca acggcgcatt
     9661 cgacacatac aatacatctt tattcttcac aggaaagcgc tgcatagaaa atagcgaatg
     9721 cgcatcagcc atattcacac gatataccgt tggaatctct gcctcactgc cataaccttg
     9781 agcaatccat ttatcctgac gttctgccgg caattccacc aatggcgtat agcggaacac
     9841 aaacacacca cgcgcatcag aacggcgatc ttgcaaaccg cccatacggc caatggcttc
     9901 agaaagcgat aagcctctgg ctgaaaaacc gatttcttgt gttctcccca ccgcacccat
     9961 agacgtaaag gtatagggat tggtaatcat ggtaaccaca tcaccgcgac gcagcaaaat
    10021 attttgtcgc ggatttgcaa ctaaatcttc caaggcaaca gttcgtacta cattgccacg
    10081 tgtcagctgc acattcgtat cctgcacatt tgccgttgaa ccacctaccg cagccaccgc
    10141 atccaacaca cgctcaccgg ctgccgtcaa cggcatacgc acactattgc ctgcgcgaat
    10201 caccgataca tttgccgcat tattctgcac caagcgcacc atcacttgcg gctgattggc
    10261 catttttttc aggcggcctt taataatttc ctgaacctga ccaggcgttt taccgaccac
    10321 cgaaatatcg ccaacaaacg gcacagaaac cgtaccacgt gccgtcacca gttgctccgg
    10381 caacttggtt tgttgcgcac tacccgagcc catcgaagaa aggccaccac caaacaatac
    10441 cgctggcggc gcttcccaaa tcataatatc caatacatca ccaatattta gcgtaccggt
    10501 agaagcataa ccatcgccaa actgggtaaa agactgattc acctgagctt tatacaaaga
    10561 ctgcgctaca gcatgattga cgtcaatcaa ctctacttca ggaatttgag cttcagactg
    10621 ttgccccaat gagacaactt tttttgcact tgggccagag gagggaatag cactacatgc
    10681 acttcccaaa attaaaaaag aaattacaat acaaaacttt aacttaagca taaaataaaa
    10741 aatctcatta agtatgattg tttttaaata aatttaaaac ctaccagaga tacaatacca
    10801 ctttattttg tagaacacaa acgtgtataa tatataacat aaacatcatc ttcgaaataa
    10861 tattggggct taggaagcaa aatcatcaaa aaacgtgata agctcctaat atttttaaca
    10921 cattactata ttacacatag gatattccaa tgaaagtctt aaccgtcttt ggcactcgcc
    10981 ctgaagctat taaaatggcg cctgtaattc tagagttaca aaaacataac acaattactt
    11041 caaaagtttg cattactgca cagcatcgtg aaatgctaga tcaggttttg agcctattcg
    11101 aaatcaaagc tgattatgat ttaaatatca tgaaacccaa ccagagccta caagaaatca
    11161 caacaaatat catctcaagc cttaccgatg ttcttgaaga tttcaaacct gactgcgtcc
    11221 ttgttcacgg agacaccaca acaacttttg cagctagcct tgctgcattc tatcaaaaaa
    11281 tacctgttgg ccacattgaa gcaggcctga gaacttataa tttatactct ccttggccag
    11341 aggaagcaaa taggcgttta acaagcgttc taagccagtg gcattttgca cctactgaag
    11401 attctaaaaa taacttacta tctgaatcaa taccttctga caaagttatt gttactggaa
    11461 atactgtcat agatgcacta atggtatctc tagaaaaact aaaaataact acaattaaaa
    11521 aacaaatgga acaagctttt ccatttattc aggacaactc taaagtaatt ttaattaccg
    11581 ctcatagaag agaaaatcat ggggaaggta ttaaaaatat tggactttct atcttagaat
    11641 tagctaaaaa atacccaaca ttctcttttg tgattccgct ccatttaaat cctaacgtta
    11701 gaaaaccaat tcaagattta ttatcctctg tgcacaatgt tcatcttatt gagccacaag
    11761 aatacttacc attcgtatat ttaatgtcta aaagccatat aatattaagt gattcaggcg
    11821 gcatacaaga agaagctcca tccctaggaa aaccagttct tgtattaaga gatactacag
    11881 aacgtcctga agctgtagct gcaggaactg taaaattagt aggttctgaa actcaaaata
    11941 ttattgagag ctttacacaa ctaattgaat accctgaata ttatgaaaaa atggctaata
    12001 ttgaaaaccc ttacgggata ggtaatgcct caaaaatcat tgtagaaact ttattaaaga
    12061 atagataaaa tgtttatact taataacaga aaatggcgta aacttaaaag agaccctagc
    12121 gctttctttc gagatagtaa atttaacttt ttaagatatt tttctgctaa aaaatttgca
    12181 aagaatttta aaaattcatc acatatccat aaaactaata taagtaaagc tcaatcaaat
    12241 atttcttcaa ccttaaaaca aaatcggaaa caagatatgt taattcctat taattttttt
    12301 aattttgaat atatagttaa aaaacttaac aatcaaaacg caataggtgt atatattctt
    12361 ccttctaatc ttactcttaa gcctgcatta tgtattctag aatcacataa agaagacttt
    12421 ttaaataaat ttcttcttac tatttcctct gaaaatttaa agcttcaata caaatttaat
    12481 ggacaaataa aaaatcctaa gtccgtaaat gaaatttgga cagatttatt tagcattgct
    12541 catgttgaca tgaaactcag cacagataga actttaagtt catctatatc tcaattttgg
    12601 ttcagattag agttctgtaa agaagataag gattttatct tatttcctac agctaacaga
    12661 tattctagaa aactttggaa gcactctatt aaaaataatc aattatttaa agaaggcata
    12721 cgaaactatt cagaaatatc ttcattaccc tatgaagaag atcataattt tgatattgat
    12781 ttagtattta cttgggtcaa ctcagaagat aagaattggc aagagttata taaaaaatat
    12841 aagcccgact ttaatagcga tgcaaccagt acatcgagat tccttagtag agatgaatta
    12901 aaattcgcat tacgctcttg ggaaatgaat ggatccttca ttcgaaaaat ttttattgtc
    12961 tctaattgtg ctcccccagc atggctagat ttaaataacc ctaaaattca atgggtatat
    13021 cacgaagaaa ttatgccaca aagtgccctt cctactttta gctcacatgc tattgaaacc
    13081 agcttgcacc atataccagg aattagtaac tattttattt acagcaatga cgacttccta
    13141 ttaactaaac cattgaataa agacaatttc ttctattcga atggtattgc aaagttaaga
    13201 ttagaagcat ggggaaatgt taatggtgaa tgtactgaag gagaacctga ctacttaaat
    13261 ggtgctcgca atgcgaacac tctcttagaa aaggaattta aaaaatttac tactaaacta
    13321 catactcact cccctcaatc catgagaact gatattttat ttgagatgga aaaaaaatat
    13381 ccagaagagt ttaatagaac actacataat aaattccgat ctttagatga tattgcagta
    13441 acgggctatc tctatcatca ttatgcccta ctctctggac gagcactaca aagttctgac
    13501 aagacggaac ttgtacagca aaatcatgat ttcaaaaaga aactaaataa tgtagtgacc
    13561 ttaactaaag aaaggaattt tgacaaactt cctttgagcg tatgtatcaa cgatggtgct
    13621 gatagtcact tgaatgaaga atggaatgtt caagttatta agttcttaga aactcttttc
    13681 ccattaccat catcatttga gaaataagtt aaattatgaa gaacctttga gtgcaattcg
    13741 aaggttcttc attcatatta ttcatatttt ggagaaatta tgttatctaa tttaaaaaca
    13801 ggaaataata tcttaggatt acctgaattt gagttgaatg gctgccgatt cttatataaa
    13861 aaaggtatag aaaaaacaat tattactttt tcagcatttc ctcctaaaga tattgctcaa
    13921 aaatataatt atataaaaga ttttttaagt tctaattata cttttttagc attcttagat
    13981 accaaatatc cagaagatga tgctagaggc acttattaca ttactaatga gttagataat
    14041 ggatatttac aaaccataca ttgtattatt caattattat cgaatacaaa tcaagaagat
    14101 acctaccttt tgggttcaag taaaggtggc gttggcgcac ttctactcgg tcttacatat
    14161 aattatccta atataattat taatgctcct caagccaaat tagcagatta tatcaaaaca
    14221 cgctcgaaaa ccattctttc atatatgctt ggaacctcta aaagatttca agatattaat
    14281 tacgattata tcaatgactt cttactatct aaaattaaga cttgcgactc ctcacttaaa
    14341 tggaatattc atataacttg cggaaaagat gattcatatc atttaaatga attagaaatt
    14401 ctaaaaaatg aatttaatat aaaagctatt acgattaaaa ccaaactaat ttctggcggg
    14461 catgataatg aagcaattgc ccactataga gaatacttta aaaccataat ccaaaatata
    14521 taaaatgcgt aagattactt ttattatccc tataaaacag tctttaataa aacctgattg
    14581 ctttatacgc ctctttttta atttattttt gctaaaaaaa ttctcaagta aatacggatt
    14641 ttctatatta gttgcagaca acagtaactt cctttggaaa aatattatta aattaattac
    14701 aaaattttac aaatgtaatt atattagtat taaatctcat aatacttttt atacgcctgc
    14761 taaaattaaa aatgcagctg ccatctatag ttttaatacc ttgaattcaa attacatttt
    14821 attcttagat gttgacgttt tattatcgga aaattttatt caacatttaa taaaaaaaac
    14881 aaaaaccaat atcgcctttg attggtaccc tgtttcattc ttaaacaaac aatttgggat
    14941 tataaatttt atattattct catataaagg taatctaaat atagaagaat cattcattat
    15001 acaaacaggg tttgtaactg gcttacaatt atttaattct gattttttct acaaaacagc
    15061 tggatacaat gaaagctttc ttggctatgg ctgtgaagat attgaaatga ttcacagagc
    15121 aacattatta ttaaatatta gacctgcctt taatgaaaat catcaatatt ttacagatga
    15181 tagaggatat atgccttcta aattaaccgg atttcgaaat tatttttatt atttgaaaag
    15241 agatgaattt tcaaacttac agataactcc taaacatttc tggcataagc gaaaaaataa
    15301 atcaaaatat ctaaaaaata gatatcaaaa tgatgtaaaa atgattcaga ttatgaaaga
    15361 ttttgatcga aaatttctaa aaaattaacg agctgtcttg cccatatgaa tcctgattac
    15421 tttaatttaa ttatgaaaaa tattctcgtt accggcggca ccggttttat cggctcgcac
    15481 accgttgttt ctttgctgaa aagcggccat caagtcgtga ttttggataa cctatgcaat
    15541 tccagcatca atatcctgcc acgcttgaaa acgattaccg gccaagagat tccgttttat
    15601 caaggcgata tccgcgatcg tgagattttg cgccgtattt ttgcggaaaa ccgcattgat
    15661 tcggtgattc attttgccgg cttgaaagcg gtgggtgaaa gtgtggccga gccgatgaaa
    15721 tattatgata ataatgtttc cggcagcttg gtgttggcgg aagaaatggc gcgtgcgggc
    15781 gtgtttagca ttgtgttcag ttcttcggcg acggtttatg gcgatccggg caaagtgcct
    15841 tataccgagg atatgccact gggcgatacc accagccctt acggcacatc gaaatcaatg
    15901 gttgagcgca ttctcactga cattcaaaaa gccgatccgc gctggagcat gattttgttg
    15961 cgttatttca atccaattgg cgcgcatgaa agcggcttga ttggcgagca gccaaacggt
    16021 atcccaaata atttgttgcc ttatatctgc caagtggcgg caggcaaact gccgcaatta
    16081 gcggtattcg gcgatgacta ccctacccct gatggcacgg gtatgcgcga ctatattcat
    16141 gtgatggact tggctgaagg ccatgtcgcc gccatgcagg cgaaaagcaa tgtagcaggc
    16201 acgcatttgt tgaacttagg ttccggtcgc gcttcttcgg tattggaaat catccgcgca
    16261 tttgaagcgg cttcgggttt aaccattcca tttgaaatca aaccacgccg tgcgggcgat
    16321 ttggcgtgtt tctatgctga cccttcttat acgaaggcac aaatcggttg gcaaactcag
    16381 cgtgatttaa gccaaatgat ggaagactca tggcgctggg tgagtaataa tccgaatggc
    16441 tacgacgatt aagttgacct gatacaggcc gtctgaaaga gatgttttca gacggcctct
    16501 ttatctgaaa aacacacatt ctgtctgcta taatctgttt atattttttg gctatcctct
    16561 gaaatttatg agaaaaatcc t
//
LOCUS       DAWKOD010000030        16536 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000030 DAWKOD010000000
VERSION     DAWKOD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.030"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(191..526)
                     /locus_tag="WJK73_000740"
     CDS             complement(191..526)
                     /locus_tag="WJK73_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ7557255.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(607..2736)
                     /gene="dnaX"
                     /locus_tag="WJK73_000741"
     CDS             complement(607..2736)
                     /gene="dnaX"
                     /locus_tag="WJK73_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ7557256.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALRNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSVQTAEKEAAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1508..1547)
                     /gene="dnaX"
                     /locus_tag="WJK73_000741"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2831..4807)
                     /gene="mutL"
                     /locus_tag="WJK73_000742"
     CDS             complement(2831..4807)
                     /gene="mutL"
                     /locus_tag="WJK73_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ7557257.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQKDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHEITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGHRLTLPEMNVLLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4951..5136
                     /locus_tag="WJK73_000743"
     CDS             4951..5136
                     /locus_tag="WJK73_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ7557258.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5147..5815
                     /locus_tag="WJK73_000744"
     CDS             5147..5815
                     /locus_tag="WJK73_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ7557259.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5815..6531
                     /locus_tag="WJK73_000745"
     CDS             5815..6531
                     /locus_tag="WJK73_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ7557260.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWLSL
                     IQTYPGSPAAKRAAAAIRKR"
     gene            6579..7064
                     /gene="purE"
                     /locus_tag="WJK73_000746"
     CDS             6579..7064
                     /gene="purE"
                     /locus_tag="WJK73_000746"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ7557261.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(7247..8701)
                     /locus_tag="WJK73_000747"
     CDS             complement(7247..8701)
                     /locus_tag="WJK73_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ7557262.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8698..9399)
                     /locus_tag="WJK73_000748"
     CDS             complement(8698..9399)
                     /locus_tag="WJK73_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ7557263.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(9396..10175)
                     /locus_tag="WJK73_000749"
     CDS             complement(9396..10175)
                     /locus_tag="WJK73_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ7557264.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAKRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10459..11865
                     /locus_tag="WJK73_000750"
     CDS             10459..11865
                     /locus_tag="WJK73_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7557265.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            11977..13503
                     /locus_tag="WJK73_000751"
     CDS             11977..13503
                     /locus_tag="WJK73_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7557266.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            13711..14244
                     /locus_tag="WJK73_000752"
     CDS             13711..14244
                     /locus_tag="WJK73_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7557267.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14287..15588)
                     /gene="aroA"
                     /locus_tag="WJK73_000753"
     CDS             complement(14287..15588)
                     /gene="aroA"
                     /locus_tag="WJK73_000753"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ7557268.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            15980..16456
                     /gene="greA"
                     /locus_tag="WJK73_000754"
     CDS             15980..16456
                     /gene="greA"
                     /locus_tag="WJK73_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ7557269.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3480 a         4473 c         4602 g         3981 t
ORIGIN      
        1 ctattcttgc cgtttttgcg ttcttatcat tctcactgtt tttaccgttc acgccgaatc
       61 ctgcaaactg atgccgtcat tcccgcgaag gcgggaatcc agacccgtcg gcacggaaat
      121 ttatcgagta aaacggtttc ttagattcta cgttctagat tcccgcctgc gtgggaatga
      181 cggtcgggga tcagcggaag aagtcgccca ctccgggggg gagtccttgg gcgaatgcgc
      241 ccattgtttt gtttgcggtt tcttcggctt tgccacgggc ggatttgagg gcggcgagga
      301 tgaggtcttc aagcatttct ttgtcgtcgg cggcttcttg aatcaaatcg gggctgatgt
      361 cgattttgcg tacttcgtgc gcgcaggtca ttgtgatttt gaccaagccg ttgcctgctt
      421 cgccttcgat ttcggtttcg gcaagtttgg cttgcgcttt tttcatattt tcctgcattt
      481 gctgcgcctg tttcatcagg ccgcctaatc cggcttttcc gaacatactg aatactcctg
      541 tttgtgttta aatgaatgag ggaaacggca atgccgtccg aagggttcgg gcggcattat
      601 atctgtttat ggccggtttg ccgccaattc cagtgattcg ggctgccatt gcgcgccgaa
      661 tgcttggagg attttttgtg cggcggggtc ggcttcgagc aatgcttgtg ctttttgcct
      721 gtcctcggct tggacgcgct tgtcctgcat cgcgggggtt tctctgccgg attcgtcctg
      781 ccagtcttgg gtttgcaggg tgagttgcag cccgtaggct tgggcaaggg tgtcgcggat
      841 tttgtcgaga cgttttttgt cggcggtggc gcgtgcttcg gcggtcattg ccaaaaccat
      901 caggccggtg tcgggatggt attccgtcca cgcggagtgt tgcgccggca tttgcgccgc
      961 gccgagtttg cgggcgaaat gccggacgat ggctgcccag ttttcggtgg aaaactcggg
     1021 cggtggggcg gacggtgtgt tgtttccgcc gatgccgtct gcttctgctt cttcgtccgc
     1081 gcctccgcct gcggtatcgg caggggcggc gtgttcccaa tcgggcggcg ggatttccgc
     1141 gttgtcttct acggggcagt catcatccgg aaagctgtaa ccgttgaaag gttttgcagg
     1201 agcttcgtgc gcaaatgctt ccgtttcaag ggcttcatta ttcggtgttg tctgaatggg
     1261 gttttcagac ggcacttcgg acaagggggc attggtttcg ttgtcggctg ccttgttctt
     1321 ggaaacttgg ttttcgggcg gcgtttcggc ttcggatgcc gtctgaatgc tttttgccga
     1381 cgtttgcgcc gtgccggctg cggcttgggc ttcgtctggc gcgtcttccc aaggcggaac
     1441 atcgttgttt tcttgattgg aaacgggttc ggcggttttg ccttcagacg gcattgccgc
     1501 tgcggatgcc gtctgaacgg atgtttgggc ggtttccgct tcagggcgcg gttggggctt
     1561 ttttgcggcg gcttcctttt cggcggtttg taccgatggg gattgaagtt cggtattttc
     1621 aatcacggcg tttgcatcac acgaagctgc cgccaagggc gcaaacgcca gcatacgcag
     1681 cagggtcatc ataaagccgg cgtattcgtc gggggcgagg cccaagtcgc gtttgccgtg
     1741 gacggcgatt tggtagtaaa gctggatttg ttcgccacct atggtttggg cgaggcggtg
     1801 caaaatatcg gaatcggggt cgtcgtgcgc caaggcattc ggcactgcct gtatcagggc
     1861 gagttgttgc agcagtatgg cgagttcgcc caaggcgttg tcaaagccga cggcacacgc
     1921 cgccatttcc cgcgctttgg cggtcagggc tgcgccgtct tggttgatga tgcctgtcag
     1981 cagttcgtaa aggtattgtt tgtcaaccgc gccgatcatt tggcggacat cgttttcggc
     2041 aactttgccc gaacccaggg cgatggcttg gtcgagcagg cttaaggcat cgcgcatcga
     2101 tccggcggcg gcgcgtccca aaagttgcag ggcggcgggt tcgtaggcga ttttttcgct
     2161 gtcgaggacg tgggcgaggt ggtcggcaac ctgctgcgcg gtcatattgc gcaagacgaa
     2221 ttgcaggcag cggcttaata cggtaacggg gactttgtgc ggatcggtcg tggcaaggat
     2281 gaatttgacg tgttcgggcg gctcttccag cgttttgagc atagcgttga acgcgctttt
     2341 ggaaagcata tgcacttcgt cgatgatata gactttgtat ttgccggcag tcggcgcgta
     2401 ttgggcgttt tccaagactt cgcggatgtt gtcgatgcct gtgttggagg cggcgtcgat
     2461 ttcgagcagg tcgacatagc gtccggcatc gatttgggtg cagctttggc acacgccgca
     2521 aggttcgccg tgttgcgcgt tttcgcagtt gaggcttttg gcaaggatgc gggcgatggt
     2581 ggttttacct acgccgcgcg taccggtcag caggtaggcg tggtgcagcc tgccttcgtc
     2641 cagcgcgttg cgcaaggctt tgacgacgtg ttcctgaccg actaagtcgg caaaggtttt
     2701 cggacgccat tttcgggcga gaacttgata ggccatgttt ttctcttggt ttcggtcgtg
     2761 atgtttctgt cggtgcttcg gaatgccgtc tgaacggcgg tctcgggcgg cgtattctag
     2821 cactttcggc ttattgtccg cgcaagaaca gtgcgtccaa ttctttcaaa gtcagtttga
     2881 cccacgtcgg cctgccgtgg ttgcactggt tgctgcgcgg cgtgttttcc atatcgcgca
     2941 gaaggacgtt catttcgggc agggtaagcc ggtggccggc gcggatcgag ccgtggcagg
     3001 acatggtggc gaggatgcgg ttttcgtgtt cctcgatggt ttggctgctg ccgacttggg
     3061 cgagttcgcc caatacgtcg cgggcgaggg agacgacatc ggatttgccc aacattgcgg
     3121 gtgcggcgcg gacggcgagg gtgttgccgc ccatatcgga taattccagc ccgaagcctg
     3181 ccagcgtttc ggcataatcg gcaagggcgg cgcattcttc gtgggacgcg gcaaaggtta
     3241 cgggaataag caggcgttgg ctttgcagcc tgccgttttc ctgacgctgg cgtttcattt
     3301 tctcgtagtt gacgcgttcg gcagcggcgt gcatatcgat gagcagcagg ctgtcttcgg
     3361 cttgggcaag aatgtagatg ccaagtaatt gggcaatggc gaaaccgagc ggcggcagtt
     3421 cggattggga ggggatgccg tctgaaagcg gcgtatctgt ttggggagca ggcgtttcag
     3481 acggcatatt gccgaaacgt gcctgctcga atcggcttaa ttcaaggtcg atgtcgtcgg
     3541 tttttttgta aagctcggca taggtgttca ttgccgcgcg gctttcgcgc agggacaggc
     3601 tgcgttgttg cggcgcacgc gcggcttggt agggcatagg cgcggttttg cctgatgcac
     3661 caaaggcatt gcgcgtatcg ggtttgttgc ctgtcgggta gtcggatgcg cgattaaaca
     3721 gattttcgct gtcgttttca gacggcattg gtgcaggcgt aactcccgtg atttcgtgca
     3781 acacttcgcc cgcgttgccg acgctttcgg tcaggtcggc gcgcgtgtcg gcaagggctt
     3841 tgttgagcgt gtggaacacg agttggtgta cctgctgact gtcgcggaag cggatttcgg
     3901 ttttggtcgg gtggacgttg acatctacgg cttcgggcgg caggtcgaga aagaggacga
     3961 aagcaggtgt cagcgcgttg tgcaatacgt cgcggtatgc ctgcttgaca gcatggagca
     4021 tgactttgtc gcggacaaaa cgatggttga caaagcagta ttgtttgtcg gttttgcctt
     4081 tggcgaaagt cggcttggca atcgcgccat agagccgcaa tgcgccattg ccgctgtcga
     4141 tttccaagga tgccgtctga aagtcatcgc cgacaatggc ggcaatccgt tcatgcaggc
     4201 tttgtgcagg gagtttgaac acttgtttgc cgtcgcgttt gagtgagaag gcgatgtgcg
     4261 gatgcgccag agcaagtcgt tcgagcatgg tggcgcagtg ggcgtattcg gtgttttccg
     4321 atttgaggaa cttgcgccgc gcgggggtat tgaagaagag ttcggcagct tcgatggtgg
     4381 tgccgacggg gtgggcggct gccgtagggc tgctgagttt gccgtcttcg gctttgactt
     4441 gggtcgcgtg cgaactgtct ttttgacggc tggtcagggt caggcggctg acggaggcga
     4501 tgcttgccaa gccttcgccg cgaaagccca tgctggcaac gtgttccaaa tcgtttaagg
     4561 ttttgatttt gctggtggcg tggcggtgga gcgcgagttc gatgtcgtct ggatggatac
     4621 cgctgccgtt gtcgctgacg cgaatcaggc ggatgccgcc gcccgccagt tcgacttcaa
     4681 ttgctgttgc gcctgcatcg atactgtttt caacgatttc tttcagggcg ttggcagggc
     4741 gttcgaccac ttcgccggca gcgatttggt tgacaagatg gtcgggcagg gcggcgattc
     4801 gggacataag gcgggcttcc gttgcaaaaa acgtcttatt ctataataaa accccttatc
     4861 tttctgcccg tattcttgat aaaagccgtt tatcccgttt ggaaaatacc agtataatca
     4921 cgccatattc cgtaaaattg gagcacaaag atgtatcact accaatccga tgccacacaa
     4981 ttcctcaacc gcctgattga agaaaaacct gagttggaac agcagcgttt ggaaaacagg
     5041 gggcttttgt gggatgtcga actcaatccc gaagaacagg aaaacttcga ggcggcaaaa
     5101 gtagcgaaaa aaccttatac ctattaccaa gactgaaccc gccgaaatga cgacccatct
     5161 gtccaatgtc gcaccggacc tgcaaaacta tctgaacagc atcggcgaac ccgaacatcc
     5221 cgttttgacg cggctgcgtg agaagaccga gcatcaccgt atgggcaaaa tggcgattgc
     5281 gcgcgaacag gcggcggttt tggtttggct ggcaaagctg attcgcgcgg aaaaatatct
     5341 ggaagtcggc gtgtttaccg gatacagcag caccgcgctt gcattggcgt tgccagaaca
     5401 cgggcggatt accgcctgcg acatcaatgt aacctttacc gatacggcgc gtcaggtttg
     5461 gaacgaggca ggtgtggcac ataaaatcag cctgcacctg caacccgcat tgctgacatt
     5521 ggatgatttg attgtacagg gtgaagccgg aagctacgat ttggcactga tagacgcaga
     5581 caaaccgccc acgccgcaat atttcgagcg ttgcctcaaa ctcgtccggc aaggcggcat
     5641 cattgccatt gacaatattt tgttgaacgg aagggtgatg cgcgaagcgg cttccgacgc
     5701 gccgcccagt gtcggcatcc tcaaagattt caatcaaaac ctgccgaacg acccgcgcat
     5761 cgtccccatc accctgcccg tcggcgacgg tttgaccctg cttctgaaaa aataatgaag
     5821 accaaattac cgctttttat catttggctg tccgtatccg ccgcctgttc ttcccctgtt
     5881 tcccgcaata ttcaggatat gcggctcgaa ccgcaggcag aggcaggtag ttcggacgct
     5941 attccctatc ccgttcccac tctgcaagac cgtttggatt atctggaagg cacactcgtc
     6001 cgcctgtcga acgaagtgga aaccttaaac ggcaaagtca aagcactgga gcatgcgaaa
     6061 acacaccctt ccagcagggc atacgtccaa aaactcgacg accgcaagtt gaaagagcat
     6121 tacctcaata ccgaaggcgg cagcgcatcc gcacataccg tcgaaaccgc acaaaacctc
     6181 tacaatcagg cactcaaaca ctataaaagc ggcaggtttt ctgccgctgc ctccctgttg
     6241 aaaggcgcgg acggaggcga cggcggcagc atcgcgcaac gcagtatgta cctgttgctg
     6301 caaagcaggg cgcgtatggg caactgcgaa tccgtcatcg aaatcggagg gcgttacgcc
     6361 aaccgtttca aagacagccc aaccgcgccc gaagccatgt tcaaaatcgg cgaatgccaa
     6421 tacaggcttc agcaaaaaga cattgcaagg gcgacttggc tcagcctgat acagacctat
     6481 cccggcagcc cggcggcaaa acgcgccgcc gcagccatac gcaaacgata gtttttccat
     6541 ttttccgctt tttaccccat ccgcaccaag gaggatttat gatacaaatc ggcatcatca
     6601 tgggcagcaa cagcgattgg cccgttatgc ggcaggcagc gcagtttctt gaagagttcg
     6661 gcgtagaata tgaggcgcgc gttgtttccg cacaccgcac cccggatttg atgttccaat
     6721 acgccgaaac cgcacgggcg cgcggcatca aagccatcat cgcgggcgcg ggcggtgcgg
     6781 cgcatttacc cggtatggtt gccgccaaga ccaccgtccc cgttttgggc gtacccgtcc
     6841 ccagcaaata cctgcgcggc gaagattcgc ttttatcgat tgtacaaatg cccaaaggcg
     6901 tacccgtcgc cacattcgcc atcggcgagg caggcgcggc aaatgccgca ttgttcgccg
     6961 tttccatgct ggccaacgaa aaccccgaac tggcgcaaaa actggcagac ttccgagcca
     7021 aacaagaaca gactgttttg aatatggaat tggaacagat ttaaaacccg ccgtccgcga
     7081 atatatagtg gattaaattt aaaccagtac ggcgttacct cgccttgccg tactatttgt
     7141 actgtctgcg gcttcgtctc cttgtcctga tttttgttaa tccactatac aaatgccgtc
     7201 tgaagccctg tttcgggttt cagacggcat ttttgcgaac aactttttat gcctgttctt
     7261 tttggcgcat tttttctgcc atcagttcgt gcagggtttt cttcgccggt ttcatcggca
     7321 cgcggttttg cgtccaaccc aactgtttgc gcggggtcag gttgcggaac ttggtggctg
     7381 cccaaccgaa ggcgcggtag gttttgctgc cgctgaaaat accgttgaat gtgcgccacg
     7441 ccatttgttc gccgaaggta tgcgatgcgc cttgccctcg gatggggtgc ggcacggttt
     7501 cggtcggcga acgttgcgct tcaacgcgca aacgctgcat ttgttcggta atcgggatgc
     7561 gtaccggaca gacttccaca cacgcaccgc acatggtaca ggcggtcggc agatcgcggg
     7621 tggcatcaag acccaacagg tgaggggaaa taatctcgcc aatcgggccg ggataggttg
     7681 tgccgtatgc cgcgccgccg atgcgggtat aaaccgggca atggttcata cacgcgccgc
     7741 aacggataca ttgcagggtg cggcgcattt ggtcttcggc ataagcctgg ctgcggccgt
     7801 tgtcgagcag aaccaagtgc atttcttgcg gaccgtctaa ttcctcactg cggcgcggac
     7861 cggtaatcat gttgaaataa gtggtaatgt tctgaccgat ggcagaacgc ggcagcagac
     7921 tgtacaaggg tggaatatcc gacagcttgg caactacttt ttcaatgccg gtaatggcga
     7981 tatgcacggg cggaacggtg gtactcaagc gaccgttgcc ttcgttttcc accagacaca
     8041 gcgtacctgt ttcagcaacg gcgaagttca cgccactcaa accgacatcg gcagtgctgt
     8101 aaatatcgcg cagtgcttta cgggcgaagc cggtcagttg gtctacgtcg tccgttaacg
     8161 gtgtgccgag gttttggtgg aacagttcgc taacctgttc tttggtttta tggattgcgg
     8221 gcatcacgat atgggtcggt ttttcacctg ccatttggac gatgaactcg cccaagtcgc
     8281 tttccaccgc tttaatgcct tttgcttcaa gataatggtt cagctcgatt tcctcgctga
     8341 ccatcgattt gcctttgacc atcagcttgc cgtttttggc tgtgatgatg tcgtggatga
     8401 tttcgcaggc ttcggccggt gtttccgccc agtgtacttt tacgcccaac ttggtcaggt
     8461 tttcttccag ctgctccagc agggcaggca atttagacaa tgagcgctga cggacgtgtt
     8521 cgcacaaatc gcgcaagctt tgcagctctt cttcgtcggt caaaacagct ttgcgtttgg
     8581 tcatcagcat atccatcgcg gtacgcaggc ttttacgcaa aggcttgtct tgaagggaaa
     8641 ttgcggcgtt ttgcttgaaa gtttccggct tcatgtgaaa tttgatggtt tgcgtagtca
     8701 tgcgttttcc tccaaatcgg taggggaaat gtggtcgggc aggatggcga ggataaccaa
     8761 atcacgcgga ccgtgcgcgc cgtaagcaag cgtcagttgg atgtctgcgg ttttggacgg
     8821 accggaaatc aggaatacat tggtcggcat accgttttcc accagttttt cgccttcgac
     8881 ggcattatga aactcgttgt acatcttgga cgtatcgaac aggcagaaat gcacgggcgg
     8941 aacgagactc aaagtacgcg gttcttcggg gctggaaaac agcatcagcg tgccggtgcg
     9001 ggcgatgccg cattgcgcgc cgctgaagcc cgcattgatg ttcgtgaaaa actcggtttt
     9061 ccaagtatcg atttcgcgct cgaagacaat cggttcgata ttgctgcccg ccaatgcggc
     9121 acgggcaatt tgtccgtgtt cggtcgccaa gggcagcagg atgtttttca aacccttgcc
     9181 ttctgccgct tcgcggaaaa cctgcatcca attgcttttc gtcacccaat aaatttcggt
     9241 tttgaccgca cgcatagcgg cagcccaatg tttcagacgc tcaacttcgc tgccccaaga
     9301 aacacccatt tcacggtaat aatcaaaaac cgcaggttct tccatcggca atgcgtcggc
     9361 ttttttcagt tttgccaaaa tattttcgcg cgcgctcatg ctttgcctcc ggtacgttcc
     9421 aacaagaagg atgcgatatg tttcggacgc ggcatattcg gttcgtcctt agcaattttg
     9481 ccgccaatgt tcatcatgca gccgcagtcc gcgctgatga tttcggtcgc gccggtttct
     9541 ttcagcgcgg cgactttgtc tgttaccatt gcgccggaaa tatcggcttg tttgacggag
     9601 aatgtgccgc cgaagccgca acattcgctt tcgtggtcgt ggacgatgcg ttcgacgttt
     9661 tccataccgt caatcagttg ccagcctgaa agatggacat tcatttcgcg gcgggcggcg
     9721 caggaagtgt gaacggcgac tttgagcggt tcgcccttgt cttcgggttt gaaaccgatg
     9781 gcaagcagga aatgggtaaa ctcgatgatg cgcttggcgc aatccacagc cctttcctca
     9841 tactcactgc ctttaaacag cgtcggccaa tggtgcttca tcatgccgcc gcatgagccg
     9901 gacggtacga caatcggcca gttctcgggg aaaaggtcga gttgcgcttt ggcgacatcg
     9961 aaggcttcgg tcggatggcc ggatgaatag gcaggctggc cgcagcagct ttgcgccatc
    10021 gggaaatgga cgcgtatgcc ctgctgctcg attagggtaa tggcatccat gcctgcttcg
    10081 ggcataaaaa gatcaaggac gcaagtgccg aaaaaataga catcggtcgg attgctgtcg
    10141 tatcggatga tttttggggg aatggttgcg ctcatgtttt ttgataacgg gaaacccgtt
    10201 tttctctgta gaaaggtaag cgtttacttt aagtaattga ctgttgcggg tcaagtctaa
    10261 ttttaaaaaa taatccggtt tttcttacaa actgccccat aacgcttact gtaccttaat
    10321 ctgatggttt tcgataataa ttatcattac aatgcaatgt cggttcgttt gcttgtgaac
    10381 attcaagatg ccgactctga cggcattcag acagcatctg aaaacaataa cggcacaagg
    10441 atggggtaca atcgccctat ggaaaaaaac accctatccg cccgcgcacc atccccttgg
    10501 ctgccgcttc tgctggcaat tgccattttt atgcagatgt tggatgcgac cattttaaat
    10561 accgcactgc ctgaaattgc cgccgacctg aatgagtcgc cattgaacat gcagctggcg
    10621 gttatttcct acacgctgac ggttgccctg ctgattcctt tgagcggtta tttggcggac
    10681 aggttcggaa cgaaaaaagt ctttttcggt tcgattgccg tttttatgct cggatcggca
    10741 ttgtgcgccg catcgggttc gctgtttgaa ttgacgcttt cccgtgtcgt tcagggcatc
    10801 ggcggttcga tgctggttcc gataccgcgt ctgaccatct tgcgtgtgta cgacaagtcc
    10861 aagctgctca acgccatcaa ttatgcggtt atgcccgcat taatcggacc ggttttaggg
    10921 cctttggcgg gcggttatct ggtcgaatac gcttcgtggc actggatttt cctgctcaac
    10981 ctgcccatcg gtcttttggg tttcatattg ggacgcaaca tcatgcccga tattaaaggc
    11041 agcaatatct ctttagactt caaaggttat ctgatttttt ctgccgccgc gtgcctcttg
    11101 ttactttcgg cagaaagcct gtcgcacgcg ctgcctccgt attttgcact gttgccgctg
    11161 tgcggcggac tgctgtttgc acgccgttat ttccgacata tgaaaaccgc gtccaaaccg
    11221 atttattccg ccgacctgtt tctgatacgc actttccgtc tgggactggc gggcaatctg
    11281 ttcagccgtc tcggcatcag ctcgattcct tttctgatgc ccctgatgtt tcaagtcgct
    11341 ttcggcttcg gcgcaagcct gtcgggttgg ctggtcgccc ccgtcgccct gtcttcgctg
    11401 ctggtcaaac cgctgattgc accgctcatg aaacgtttcg gctaccgcac ggtactgctt
    11461 tggaacacca agctgcttgc cgccttcatc atgctgctcg ccctgcctga tggaaactcg
    11521 ccgctgtgga tttggatttt cctctcgctg gcgatcggcg cgtgcaactc cctacagttt
    11581 tctgccatga acacactgac cctcgccgat ttgcgcccgc aacagaccgg cagcggcaac
    11641 agcctgatgg cggtcaacca acagcttgcc atcagcatgg gcattgttgt cggcgcatta
    11701 atccttaaaa actggacatt tctgataccg gcttcttcag gtctgcattc cgccttccgt
    11761 atgaccctgc tcagtatcgg cggcattacc cttgcctcgt cgttggtttt caagcgtctg
    11821 cacgtttcag acggtgcaaa cctgacacgg aacacaccgt cctaaagcgg tccgcacgca
    11881 aaacttttac ccgtttcaac gtttggatta tgattccgca cttccatgcg cgccaacccc
    11941 aaaacacagg caatgccgtc tgaaaccata tccctgatga aaacacgcag cctaatttcc
    12001 cttttatgcc tccttctctg ttcatgttct tcatggttgc ccccactgga agaacggacg
    12061 gaaagccgtc atttcaatac ttccaaaccc gtccgcctgg acaacatcct gcaaatccgg
    12121 cacacccctc ataccaacgg gctatccgat atctatctgc tgaacgaccc ccacgaagcc
    12181 tttgccgccc gcgccgccct tatcgaatct gccgaacaca gcctcgattt gcaatactac
    12241 atctggcgca acgacatttc cggccgactg ctgttcaacc tcgtgtacct tgccgcagaa
    12301 cgcggtgtgc gcgtacgcct gctgttggac gacaacaaca cgcgcggatt ggacgacctc
    12361 ctgctcgccc tcgacagcca tcccaatatc gaagtgcgcc tgttcaaccc cttcgtcttc
    12421 cgcaaatggc gcgcactcgg ctacctgact gacttccccc gcctcaaccg ccgcatgcac
    12481 aacaaatcct ttaccgccga caaccgcgcc accatactcg gcggacgcaa tatcggcgac
    12541 gaatacttca aagtcggtga ggacaccgtt ttcgccgacc tggatatcct cgctaccggc
    12601 agcgtcgtcg gcgaagtatc gcacgacttc gaccgctact gggcaagcca ttccgcccac
    12661 aacgccacgc gcatcatccg cagcggcaac atcggcaagg gtcttcaagc actcggatac
    12721 aacgacgaaa cgtccagaca cgcgctcctg cgctaccgcg aaaccgtcga acagtcgccc
    12781 ctctaccaaa aaatacagac aggacgcatc gactggcaga gcgtccaaac ccgcctgatc
    12841 agcgacgacc ctgcaaaagg actcgaccgc gaccgccgca aaccgccgat tgccgggcgg
    12901 ctgcaagacg cgctcaaaca gcccgaaaaa agcgtctatc tggtttcacc ctatttcgtc
    12961 cccacaaaat ccggcacaga cgcactggca aaactggtgc aggacggcat agacgttacc
    13021 gttctgacca actcgctaca ggcgaccgac gttgccgccg tccattccgg ctatgtcaaa
    13081 taccgaaaac cgctgctcaa agccggcatc aaactctacg agctgcaacc caaccatgcc
    13141 gtccctgcca caaaagacaa aggcctgacc ggcagctccg taaccagcct gcacgccaaa
    13201 accttcattg tggacggcaa acgcatcttc atcggttcgt tcaacctcga cccccgttcc
    13261 gcgcgtctca acaccgaaat gggcgttgtt atcgaaagcc ccaaaatcgc agaacagatg
    13321 gagcgcaccc ttgccgatac cacccccgaa tacgcctacc gcgttaccct cgacaggcac
    13381 aaccgcctgc aatggcacga tcccgccacc cgaaaaacct acccgaacga acccgaagcc
    13441 aaactttgga aacgcatcgc cgcaaaaatc ctatccctgc tgcccataga aagtttatta
    13501 tagaaatata gcggattaac aaaaaccagt acgacgttgc ctcgccttag ctcaaagaga
    13561 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattc gtactgtctg
    13621 cggcttcgtc gccttgtcct gatttttgtt aatccactat accgtctgaa acaccttcag
    13681 acggatatcc gaacccgcaa aggaaaaacc atgtttcccc ccgacaaaac ccttttcctc
    13741 tgtctcagcg cactgctcct cgcctcatgc ggcacaacct ccggcaaaca ccgccaaccg
    13801 aaacccaaac agacagtccg gcaaatccaa gccgtccgca tcagccacat cgaccgcaca
    13861 caaggctcgc aggaactcat gctccacagc ctcggactca tcggcacgcc ctacaaatgg
    13921 ggcggcagca gcaccgcaac cggcttcgat tgcagcggca tgattcaatt cgtttacaaa
    13981 aacgccctca acgtcaagct gccgcgcacc gcccgcgaca tggcggcggc aagccgcaaa
    14041 atccccgaca gccgccttaa ggccggcgac ctcgtattct tcaacaccgg cggcgcacac
    14101 cgctactcac acgtcggact ctatatcggc aacggcgaat tcatccatgc ccccagcagc
    14161 ggcaaaacca tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa ctacctcggc
    14221 gcacatactt tctttacaga atgagccgcc cgccgcgccg cgccggaatc gcggcaaaat
    14281 gccgccttat tccgctgttt cggtcagcga tgagaacacg tcgaaataag tcgggaaggt
    14341 tttgtgggtg catttcggat cgttgatgac gacgggtacg tccaacagcg aaaccagcga
    14401 gaaacacatt gccatgcggt ggtcgtcgta agtgtcgatg acggcgtcgg gtgtcggcgt
    14461 ttcgggcggg gtgatgtgaa ttgcttcggc ttcttcggcg acttttgcac cgagtttgcg
    14521 caactcgttc gccattgcgg caatgcggtc ggtctctttg acgcgccacg aaccgatgtt
    14581 gcgaagcgtg caggtttgcc ctgtagcaag cgcgacgacg gcgagggtca tggcggcatc
    14641 ggggatatgg tttgcatcca aatcaaaggc ttggacggca cgttctttcg ggcgcgagac
    14701 ttcgacgaag ttttcgcccc aaaccacgtc cgcaccgatt ttttccagct cgcaggcaaa
    14761 ggcgacatcg ccctgtatgc tgtttgcgcc gataccggta acgcggacgg gcgtggcggc
    14821 aatcaaaccg gctgcgagga agtaggacgc gctggaggca tcgccttcga cgtgcaagtg
    14881 ttcgggcgcg tgatagtgcg catcggcggg aattttgaag acgcggtagc cttcattgat
    14941 aacctgtacg ccgaattgcg ccatcagttt taaagtaatg tcgatatagg gcttggaaat
    15001 taactcgccg accatacgga tttcaaacgc ctgcccggtc agcggcaacg ccattaaaag
    15061 ggcggtcaga aactggctgg atacattgcc tttaatcgga atcacgcgct cgccgttgtc
    15121 ttggcgttcg ccgatatgaa gcggcggata gtgttccttg ccgagatatt cgacatcggc
    15181 gccggcaatc cgcagtgcat cggccaaatc gccgatgggg cgttcgtgca tacgcgcaac
    15241 gccgtgcagg cgataatcgc cgcccaaaac ggcaagcgcg gcggtcagcg ggcggaacgc
    15301 cgtgcccgcg ttgcccaaaa acaaatcggc agtgcggttg gggaagcgtc cgcctgtgcc
    15361 gtgcactttc agacggtctt cggcaagata ttcgatttca acgccgagtt tatcgagtgc
    15421 ttcgagcata cggtcggtat cgtcggattt gagcagggaa tggatttcgc aagcattgtc
    15481 ggacaaggcg gcaagcagca gggtgcggtt gctgatgctt ttggagccgg gcagggcgac
    15541 agtggaaggt ttgagcgagg cggcagggag gcggagggat tcggtcatgg caaaacgtaa
    15601 atataaagat aaaaacagcc tgcattatac tggtgcaaat gccgtctgaa aaatctcagg
    15661 tttggcattt tcggttttaa agtccgtgaa tatggttttt tgtgccgaaa attgattatt
    15721 tttaaatttt ttgtttctaa aatttttttg ccggcatatt ttcggctttt gttgcggaca
    15781 tagcgtgttt gcggacggca cggcacgatt gccaatttca tagaatttgg tagaatagcc
    15841 gctgttcaac gacagacaag ccgccgattt tccgggcggc ttgtgttttt attttaatat
    15901 ccggacgcac aaaacaagac ggtatgcggt atgtttgctg ccgcaaaccg gtgtgccgga
    15961 atatggagaa aaaagaccga tgcaaaaaat ccccctgacc gtacgcggtg cggaattgct
    16021 gaaacaggaa ttgcagcagc ttaaaagcgt ggcgcgcccc gaagtgatcg aagcgattgc
    16081 tgaagccaga tcgcacggcg atttgtccga aaacgccgaa tatgaagccg ccaaagaacg
    16141 ccaaggcttt atcgaaggcc gcatttccga gctggaacac aaactttccg ttgcccacat
    16201 catcaatccg accgaaatcc acgccgaagg caaaatcgtg ttcggtacga cggttacgct
    16261 ggaagatttg gaaacggaag aacacgttac ctaccaaatt gtcggcgaag acgaagcgga
    16321 tatcaaacag ggcaaaatct atgtcggctc accgattgcc cgcgccttaa tcggcaagga
    16381 agagggcgat acggcggaag tccaagcccc gggcggcgtg cgcgaatacg acattatcga
    16441 agtccgatat atttgattcg gcttgatttc gatacacccg acacacgcag gaaattatag
    16501 tggattaata aaaatcagga caaggcgacg aagccg
//
LOCUS       DAWKOD010000031        16456 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000031 DAWKOD010000000
VERSION     DAWKOD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.031"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            209..1273
                     /locus_tag="WJK73_000755"
     CDS             209..1273
                     /locus_tag="WJK73_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ7557270.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WJK73_000756"
     CDS             1414..1737
                     /locus_tag="WJK73_000756"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ7557271.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WJK73_000757"
     CDS             1809..2132
                     /locus_tag="WJK73_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ7557272.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WJK73_000758"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WJK73_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ7557273.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WJK73_000759"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WJK73_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ7557274.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WJK73_000760"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WJK73_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ7557275.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WJK73_000761"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WJK73_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ7557276.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYHVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WJK73_000762"
     CDS             4530..4883
                     /locus_tag="WJK73_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ7557277.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WJK73_000763"
     CDS             4873..6687
                     /locus_tag="WJK73_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ7557278.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WJK73_000764"
     CDS             6877..7554
                     /locus_tag="WJK73_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7557279.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WJK73_000765"
     CDS             7568..8974
                     /locus_tag="WJK73_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ7557280.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WJK73_000766"
     CDS             9092..11518
                     /locus_tag="WJK73_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ7557281.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYTSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WJK73_000767"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WJK73_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ7557282.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WJK73_000768"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WJK73_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ7557283.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WJK73_000769"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WJK73_000769"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ7557284.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WJK73_000770"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WJK73_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ7557285.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13785..14510
                     /locus_tag="WJK73_000771"
     CDS             13785..14510
                     /locus_tag="WJK73_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557286.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14545..15420
                     /locus_tag="WJK73_000772"
     CDS             14545..15420
                     /locus_tag="WJK73_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ7557287.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15497..16411
                     /locus_tag="WJK73_000773"
     CDS             15497..16411
                     /locus_tag="WJK73_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ7557288.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
BASE COUNT         4267 a         5284 c         3612 g         3293 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagcaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac gcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag
     2641 ggtaatctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctattagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtagag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacggcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctaccacgtc atccaactgc ctgccggcat cgacgaatcc gttaaagccg
     4021 gcgaaacacc tttcgcttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt cccttgcttg ggaactgtgg attgcaccgt
     4621 tacgcccggg cggttcgtgg cttgcgctca aaaccctgcc cctctgcctg ccgctttcag
     4681 gcatcctgaa gaaaaaaatc tacacctacc aatacagctc catgttgatt ctgatttatt
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatccggc agaacaaatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgccc gtatccggtg cgcgccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aggcagcaac cgtcgcccga cttttaccac gatgctgcag ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cggcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc tttatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa taccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagctg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgcctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcagcca tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag cccttgcagc ggcaaatccc tccctgctcg ccgaagccct
    10441 gcacctcccc ccctaccggc acactaccca taacgccgtc caattccaat tcggcagcca
    10501 ccccctctat ggcgacatcc tgaccgcatg ctgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac cgtcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctca acggcggaca atacaccagc gatttcctct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcacacctca aaacactgtc
    10981 cctaaaaaac aacacgaccg ttaccattac cccccttttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcagccccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaaccgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcacacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccccttc tacaacgtca tcgttaccga ttcgcgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa tgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatt cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacggcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcgcggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 accattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt acccgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcgg
    12541 caatagaaca cgccaaaaca caatcctccc aagctgcaaa agtcatctac ctcagccccc
    12601 aagggaaacc gctgacacac caaaaagcgg tagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acggtatgag ggcatagacg aaaggcttct gcaaagcagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaggatt
    12841 cgttctcaag cggcattttg gattgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtattg cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcgttttaa
    13021 tcccaaagga atcccgcctc ttagaaacca tccggcaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gtggaacgta
    13321 ctttccaact gtactcccct accgttgaga aaatcgaagt caaacgccgt ggcgatgtcc
    13381 gccgtgccaa actttactat ctgcgcggcc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgtc ctgccgtaca ccacgttgac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatcc acctttctca
    13921 aatccttgat gggattgcag cctatcgata caggcagcat ccggctggat ggattgagcc
    13981 gccaagacat cgcctacctt ccccagcagt ccgatatcga ccgctcccaa cctatgactg
    14041 ttttcgactt ggcggcaatg gggctttggt atgaaatcgg ctttttcaaa gggataaata
    14101 ccgctcaaaa acaacgcgtt cacgaagcct tggagcgtgt aggaatgcgg cagtttgcac
    14161 gccgccaaat cgcccacctc tcaaacggac aattccaacg cgtccttttt gcccgaatgc
    14221 tggttcaaaa tgcaaaattc ctgcttttgg acgaaccttt caacgccgtt gatgcacgga
    14281 cgacctacga acttctcgac gtattgcaga aatgccattg cggcggacac gctatcattg
    14341 ccgtactgca cgattacgaa caagtccgtg cctactttcc cagtaccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgactggtg cgcctcataa atactgcccg
    14521 aaaccacgcc ccacaccaga caccatgaat ctctacgacc tgctccttgc cccctttgca
    14581 gaattcgact ttatgcgcta cgccctcgca tccgtcttct gcctgtccct cagtgccgca
    14641 cccgtcggcg tattcctcgt catgcgccgt atgagcctga taggcgacgc attgagccac
    14701 gccgtcctgc ccggtgccgc cgtcggctac atgtttgccg gcttgagcct gcccgccatg
    14761 ggtttgggcg gcgtagccgc aggcatgctg atggcactgc ttgccggact cgtcagccgc
    14821 ttcaccaccc tgaaagaaga tgccaacttt gctgcctttt atctcagcag cctcgccatc
    14881 ggcgtagtcc tcgtcagcaa aaacgggagc agcgtcgatt tgctccacct ccttttcggc
    14941 tctgtacttg ccgtcgatat tcctgccctg cagctcatcg ccgccgtctc cagcctcacg
    15001 ctcattaccc ttgccgtcat ctaccgcccg ctcgtactcg aaagcatcga ccccctgttt
    15061 ctcaaatccg tcggcggcaa aggcgggctt tggcacgtcc tctttctcgt cctggtcgtc
    15121 atgaacctcg tatccggctt tcaagccctc ggcacactca tgtccgtcgg actcatgatg
    15181 ctgccagcca ttaccgcccg cctgtgggcg aagcacatgg gcgcactcat cctcctatcc
    15241 gttctgacag ccctgctgtg cggcttgagc ggactgctca tttcctacca catcgaaatt
    15301 ccctccggtc ccgccatcat cctctgttgc agcgtccttt atctcttttc cgtcatactc
    15361 ggcaaagaag gcggcattct gaccaaatgg ctcaaaaacc accgccacca caccacctga
    15421 ccccgcaagc cgcttgaaca aacaggcagc ttgttttacc ataacagatg ttacgttata
    15481 tcacaggaga acagttatga aacacctcaa actcaccctt attgccgcat tgctaaccac
    15541 cgccgcaact gccgcccccc tgcccgtcgt aaccagcttc agcattttag gcgacgttgc
    15601 caagcaaatc ggcggagagc gcgtatccat acaaagtttg gtcggagcca accaagatac
    15661 tcatgcctat cacatgacca gtagcgacat taaaaaaatc cgcagtgcaa aactcgtcct
    15721 gctcaacggc ttaggacttg aagctgccga tgtacaacgt gccgtcaaac agagcaaagt
    15781 atcctatgcc gaagcgacca aaggcatcca acccctcaaa gccgaagaag aaggcggaca
    15841 ccatcatgac cacgaccatg accacgaagg acaccaccat gaccacggcg aatttgaccc
    15901 gcacgtctgg aacgaccccg tccttatgtc cgcctatgcc caaaacgtcg ccgaagccct
    15961 gataaaggcc gaccccgaag gcaaagttta ttatcaacaa cgcttgggca actaccaaat
    16021 gcagcttaaa aaactgcaca gcgacgcaca agccgcattt aacgccgtcc ctgccgccaa
    16081 acgcaaagtc ctgaccgggc acgatgcctt ttcctacatg ggcaaacgtt accatattga
    16141 attcatcgcc ccgcaaggtg tgagcagcga agccgagcct tcagccaaac aagtcgccgc
    16201 catcatccga caaatcaaac gcgaaggcat caaagccgtc tttaccgaaa acatcaagga
    16261 cacccgtatg gttgaccgta tcgccaaaga aaccggtgtc aacgtcagcg gcaaactgta
    16321 ttccgacgca ctcggcaacg cgcccgcaga cacctacatc ggcatgtacc gccacaacat
    16381 caaagccttg atcaacgcga tgaagcaata aaaccgtttg attggtttga atttgaaaag
    16441 gcaaaaggcc gtctga
//
LOCUS       DAWKOD010000032        16262 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000032 DAWKOD010000000
VERSION     DAWKOD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.032"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..123
                     /locus_tag="WJK73_000774"
     CDS             <1..123
                     /locus_tag="WJK73_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7557289.1"
                     /translation="ENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            130..205
                     /locus_tag="WJK73_000775"
     tRNA            130..205
                     /locus_tag="WJK73_000775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Trp,seq:cca)
     gene            320..598
                     /gene="secE"
                     /locus_tag="WJK73_000776"
     CDS             320..598
                     /gene="secE"
                     /locus_tag="WJK73_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ7557290.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            600..1136
                     /gene="nusG"
                     /locus_tag="WJK73_000777"
     CDS             600..1136
                     /gene="nusG"
                     /locus_tag="WJK73_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ7557291.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1237..1671
                     /gene="rplK"
                     /locus_tag="WJK73_000778"
     CDS             1237..1671
                     /gene="rplK"
                     /locus_tag="WJK73_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ7557292.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1671..2366
                     /gene="rplA"
                     /locus_tag="WJK73_000779"
     CDS             1671..2366
                     /gene="rplA"
                     /locus_tag="WJK73_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ7557293.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2596..3096
                     /gene="rplJ"
                     /locus_tag="WJK73_000780"
     CDS             2596..3096
                     /gene="rplJ"
                     /locus_tag="WJK73_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ7557294.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3154..3525
                     /gene="rplL"
                     /locus_tag="WJK73_000781"
     CDS             3154..3525
                     /gene="rplL"
                     /locus_tag="WJK73_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ7557295.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3716..7894
                     /gene="rpoB"
                     /locus_tag="WJK73_000782"
     CDS             3716..7894
                     /gene="rpoB"
                     /locus_tag="WJK73_000782"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ7557296.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8046..12221
                     /gene="rpoC"
                     /locus_tag="WJK73_000783"
     CDS             8046..12221
                     /gene="rpoC"
                     /locus_tag="WJK73_000783"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ7557297.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12512..12769
                     /locus_tag="WJK73_000784"
     CDS             12512..12769
                     /locus_tag="WJK73_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557298.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12766..12915
                     /locus_tag="WJK73_000785"
     CDS             12766..12915
                     /locus_tag="WJK73_000785"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7557299.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            13094..13465
                     /gene="rpsL"
                     /locus_tag="WJK73_000786"
     CDS             13094..13465
                     /gene="rpsL"
                     /locus_tag="WJK73_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ7557300.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13583..14053
                     /gene="rpsG"
                     /locus_tag="WJK73_000787"
     CDS             13583..14053
                     /gene="rpsG"
                     /locus_tag="WJK73_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ7557301.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14072..16177
                     /gene="fusA"
                     /locus_tag="WJK73_000788"
     CDS             14072..16177
                     /gene="fusA"
                     /locus_tag="WJK73_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ7557302.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKI
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4313 a         3614 c         4220 g         4115 t
ORIGIN      
        1 gagaacgtaa ccattaccgt agaactgatt gcgcctatcg ctatggaaga aggcctgcgc
       61 tttgcgattc gcgaaggcgg tcgtaccgtg ggtgccggcg tggtttcttc tgttatcgct
      121 taagtttaga ggccaatagc tcaattggta gagtatcggt ctccaaaacc gagggttggg
      181 ggttcgagac cctcttggcc tgccaaataa aaaattaacc ggccttgtgt cggttaattt
      241 ttttgtattt gttatttagt aaactctctt gccatttaca tggattgaga atagacagat
      301 gctatgatgg ataaataata tgacagaaca tacgcctgaa aaaaagaacg ttaaagtgga
      361 tcaactggtt gttcaagata aagaatctgc atctaattcc ggtaaggaag ggttttttgc
      421 atatttctca aattcttggt tcgaattcaa aaaggtggtt tggcctaagc gtgaagatgc
      481 tgtcagaatg actgtatttg ttatagtgtt tgttgctgtg ctttctatat ttatctatgc
      541 ggcagataca gcaatttcgt ggttattttt tgatgtattg ctgagaaggg aaggttgaga
      601 tgtcgaaaaa atggtatgtt gtacaggcgt attcggggtt tgagaagaat gtccaacgaa
      661 tattggaaga gcgcattgcc cgtgaggaga tgggagatta tttcggacaa attctggtgc
      721 ctgtagagaa agttgttgat atccgcaatg gtcgtaagac tattagtgaa agaaagtcat
      781 atcctggtta tgtgctagtt gagatggaaa tgacagatga ctcttggcat cttgtaaaaa
      841 gcaccccccg tgtttccggt tttattggag ggagggctaa tagacctacg ccgattagtc
      901 agagagaggc tgaaattatt ttacagcagg ttcagaccgg catagagaag ccgaaaccaa
      961 aagttgaatt tgaggtcggt caacaggttc gtgtaaatga agggccgttt gcggatttta
     1021 acggggtggt tgaggaggtc aattatgaac ggaataagtt acgcgtgtct gttcagatat
     1081 ttggtagaga aacacccgtt gagctggagt tcagccaggt tgaaaagatt aactgatttt
     1141 tatacttgaa aaaaaagcaa taagaggata gaatcaaaaa ttaacttggg gagcggaaat
     1201 ggttccgcgt cttacccgtt tttaggagtt cgttaagtgg caaagaaaat tatcggctat
     1261 attaaactgc aaattcctgc aggtaaagcc aatccatctc ctccggttgg tcctgctttg
     1321 ggtcagcgcg gtttgaatat tatggaattt tgtaaggcat ttaatgctgc aacccaaggt
     1381 atggagcctg gcttaccgat tccggttgtg attactgcat ttgcagataa atcattcaca
     1441 tttgtgatga aaaccccgcc agcttctatc ttgttgaaaa aggctgccgg tttgcaaaaa
     1501 ggtagttcta atcctctgac caacaaagtg ggtaaattga cccgtgccca gttggaagaa
     1561 attgctaaaa ctaaagagcc tgatttgact gctgctgact tggatgcggc tgtccgtact
     1621 atagcaggtt ctgctcgctc aatgggcttg gatgtggagg gtgttgtata atggctaaag
     1681 tatctaaacg cttgaaagct cttcgctctt ctgtggaagc caataaatta tatgcaattg
     1741 atgaagcaat tgctttggta aaaaaagcag cgactgctaa atttgacgag tctgttgacg
     1801 tatctttcaa cttgggcgtt gatccgcgta aatctgacca agttatccgt ggttcagtcg
     1861 ttctgcctaa aggcaccggt aagataactc gcgtagctgt atttactcaa ggtgcaaatg
     1921 cagaagctgc taaagaagct ggtgcagata tcgtcggttt cgaagatttg gctgctgaaa
     1981 tcaaagcagg caatctgaac tttgatgtcg ttattgcttc tcccgatgca atgcgtattg
     2041 ttggtcagtt gggtactatt ttgggtcctc gaggcttgat gccaaaccct aaagtaggta
     2101 ctgttactcc taacgttgct gaagcagtaa aaaatgcaaa agcaggtcaa gtacaatacc
     2161 gtacagataa agcaggtatc gttcatgcaa cgattggtcg tgcttctttc gctgaagctg
     2221 acttgaaaga gaactttgat gcgttgctgg atgctatcgt taaagccaag cctgctgccg
     2281 ctaaaggtca gtatctgaaa aaagttgctg tgtctagcac catgggtttg ggtattcgcg
     2341 ttgatacatc aagcgtaaat aactaatctt aaggaatttt caagcagttt ggttttctgg
     2401 gctgcttgaa tttgggctac ttaaaattaa gtagatgtcc aagaccgtag ggatcgtaag
     2461 atttaatcgt aactgcccta cgcagacggt agtcctgaaa cacattgcaa gattgcttgt
     2521 aagatgtctt tttaggttac cgcgctggtg ggatatcgtt ttggtatcct gtttataaac
     2581 agtgggaggt agaccttgag tctcaatatt gaaaccaaga aagtggcggt cgaggaaatt
     2641 agcgcggcaa ttgctaatgc tcaaaccctc gtagtcgctg aatatcgcgg tatcagtgtt
     2701 tccagtatga ctgagcttcg tgcgaatgca cgtaaagaag gcgtttattt gcgcgttctg
     2761 aaaaatactt tggctcgtcg tgcagtgcaa ggtacttcat ttgcagaatt ggccgatcaa
     2821 atggttggtc cgttggttta cgctgcttct gaagatgctg ttgctgctgc taaagtgttg
     2881 caccaattcg cgaaaaaaga tgacaaaatt gtcgttaaag ccggttctta caatggcgaa
     2941 gtaatgaatg ctgctcaggt tgctgagttg gcttctattc cgagccgcga agagctgttg
     3001 tccaaactgt tgttcgttat gcaagctcct gtatcgggct ttgcgcgcgg tttggctgct
     3061 ttggcagaga aaaaagccgg cgaagaagcc gcttaatcga ttttgtttct gttaatcaat
     3121 tattttttaa tacaatattt ggagtaaaat agcatggcta ttactaaaga agacattttg
     3181 gaagcagttg gttctttgac cgtaatggaa ttgaacgact tggttaaagc ttttgaagaa
     3241 aaattcggtg tttctgctgc tgctgttgca gttgcaggtc ctgctggtgc cggtgctgcc
     3301 gatgctgaag aaaaaaccga atttgatgtc gttttggctt ctgccggtga tcaaaaagtc
     3361 ggcgtgatta aagttgtccg tgcaattacc ggtttgggtc tgaaagaagc taaagacatc
     3421 gttgacggtg cacctaaaac cattaaagag ggtgtttcta aagctgaagc cgaagacatc
     3481 caaaaacaac tggaagaagc cggcgctaaa gtcgaaatca aataatttga tgcttcttat
     3541 gaaggctggc agttttctgc cagccttatt ttgcttctta aaataagcat taagtattgt
     3601 ttacatttat ttgcatagtt tttatcaaat cattgcaaat aaatgtaaac atcagattga
     3661 tgcgtgccgt tgctttcaga cggcctatta ttgaaaatta cttttcggag tgtatatgag
     3721 ctattcgttt accgagaaaa aacgtatccg taagagtttt gcaaagcggg aaaatgtttt
     3781 ggaagttcct ttcttgctgg caacccaaat tgattcttat gcgaagtttt tgcagctgga
     3841 aaatgctttt gacaaacgta ccgatgacgg tctgcaggcg gcatttaatt ctattttccc
     3901 gattgtgagc cataacggtt atgcgcgatt ggagtttgtg cattacacat tgggcgagcc
     3961 tttgttcgat attcccgaat gtcagttgcg cggaatcact tatgcagccc ccttgcgcgc
     4021 gcgtatccgt ttggtgattt tggataagga agcatctaaa ccgacggtaa aagaagttcg
     4081 tgaaaacgaa gtgtatatgg gcgaaattcc gttgatgacc ccgagcggtt cttttgtgat
     4141 taacggcaca gagcgtgtga ttgtttccca gttgcaccgt tcgcccggcg tgtttttcga
     4201 gcatgacaaa ggtaagacgc actcttccgg caaattgtta ttctccgccc gcatcattcc
     4261 ctaccgtggt tcatggttgg attttgaatt tgatccgaaa gatttgctgt atttccgtat
     4321 cgaccgccgc cgtaaaatgc cggtaacgat tttgttgaag gctttgggct acaacaatga
     4381 gcaaatcttg gatattttct acgacaaaga aacgttctat ttgtcttcaa acggtgttca
     4441 aaccgatttg gtcgcaggcc gtctgaaagg cgaaactgcc aaggtcgata tcttggataa
     4501 agaaggcaat gtattggttg ccaaaggtaa gcgcattact gcgaaaaata tccgtgatat
     4561 taccaatgca ggcctgaccc gtttggatgt agaaccagaa agcctgctgg gcaaagcatt
     4621 ggctgccgac ctgattgatt cggaaaccgg cgaggtattg gcttctgcca atgatgaaat
     4681 tacagaagag ttgttggcca aatttgatat caacggcgta aaagaaatta cgacccttta
     4741 catcaatgag ttggatcagg gtgcttatat ctccaatacc ttgcgtacgg atgagactgc
     4801 cggccggcag gcggcgcgtg ttgcgattta ccgtatgatg cgtccgggcg aaccgcccac
     4861 tgaagaggcg gtcgagcaat tgtttaaccg cttgttcttc agtgaagaca gctacgattt
     4921 gtcccgcgta ggccgtatga aatttaatac gcgcacatac gaacaaaaac tgtccgaagc
     4981 ccaacaaaac tcttggtacg gccgcctgct gaacgaaacg tttgccggtg ctgccgacaa
     5041 aggcggttat gtcctgagcg tcgaagatat tgtcgcctcg attgcgactt tggtcgagtt
     5101 gcgtaacggc catggcgaag tggacgatat cgatcacttg ggcaaccgcc gagtacgttc
     5161 ggtaggcgag ctgactgaaa accaattccg tagcggtttg gcccgtgtgg aacgtgccgt
     5221 aaaagaacgt ttgaatcagg cggaatcaga aaacttgatg ccgcacgatt tgattaatgc
     5281 aaaacctgtt tctgctgcta ttaaagaatt cttcggctcc agccaattga gtcagtttat
     5341 ggatcagacc aatcccttgt ctgaagtaac ccataaacgc cgcgtatccg cattgggtcc
     5401 gggcggtttg acccgcgaac gtgcaggatt tgaggtgcgg gacgtgcatc cgacccacta
     5461 cggccgcgta tgtccgattg aaacgcctga aggtccgaac atcggtttga tcaactcact
     5521 gtccgtttat gcgcgcacca atgattacgg tttcttggaa acgccttacc gccgcgttat
     5581 cgacggcaaa gtaaccgagg aaatcgatta cttgtctgcc atcgaagaag gccgctatgt
     5641 gattgcacag gcgaatgccg atttggattc agatggcaat ctgattggcg atttggttac
     5701 ctgtcgtgaa aaaggcgaaa ccattatggc aacgcccgac cgcgtccaat atatggacgt
     5761 ggcaaccggc caggtggtat ccgttgcggc atccctgatt ccgttcttgg aacatgatga
     5821 cgcgaaccgc gcattgatgg gtgccaacat gcaacgtcag gcagtgcctt gcttgcgtcc
     5881 tgaaaaaccg atggtcggta ccggtatcga gcgttccgtt gccgttgact ctgctactgc
     5941 aatcgttgcc cgccgaggcg gcgtggtcga gtatgtcgat gccaaccgcg tcgtcgttcg
     6001 tgttcatgac gatgaagcga ctgccggcga agtgggtgtc gatatttaca acttggttaa
     6061 atttacccgt tccaatcaat ctactaacat caaccaacgt ccggctgtca aagcaggcga
     6121 tgttttgcaa cgcggcgatt tggtggccga tggcgcgtcc accgatttgg gcgaattggc
     6181 tttgggtcaa aatatgacca tcgccttcat gccgtggaac ggttacaact acgaagactc
     6241 gattctgatt tccgaaaaag tggctgcgga cgaccgctat acttcgattc acattgagga
     6301 attgaatgtc gttgcccgcg ataccaagct gggtgcggaa gacattaccc gcgatattcc
     6361 gaacttgtcc gagcgtatgc aaaaccgttt ggacgaatcc ggtatcgttt acatcggtgc
     6421 ggaagtagaa gccggcgatg tgttggtagg caaggtaacg cctaaaggcg aaacccagct
     6481 gacgccggaa gaaaaactgc tgcgcgccat cttcggtgaa aaagcatctg acgtaaaaga
     6541 tacttcattg cgtatgccta ccggcatgag cggtaccgtt atcgacgttc aagtcttcac
     6601 tcgtgaaggt attcaacgcg acaaacgtgc tcaatccatt atcgattccg agttgaaacg
     6661 ctaccgtttg gatttgaacg accaattgcg tattttcgac aacgacgcat tcgaccgtat
     6721 cgagcgtatg attgtcggtc agaaagccaa cggcggcccg atgaagctgg ccaaaggcag
     6781 tgaaatcacg accgaatatc tggcgggtct gccgagcagg cacgattggt tcgatatccg
     6841 tctgaccgat gaagatttgg ccaagcagtt ggaactgatt aaagtgagcc tgcaacaaaa
     6901 acgcgaagaa gcggacgagt tatacgaaat caagaagaaa aaactgaccc aaggcgacga
     6961 attgcaaccc ggcgtacaaa aaatggtgaa agtttttatc gccatcaaac gccgtctgca
     7021 agccggcgac aaaatggcgg gccgccacgg taacaaaggc gtggtatcgc gcattctgcc
     7081 agtggaagac atgccttaca tggcggacgg ccgtccggta gacatcgtac tgaacccatt
     7141 gggcgtacct tcccgtatga acatcggtca gattttggaa gttcacttgg gttgggcagc
     7201 aaaaggtatc ggcgagcgta tcgaccgtat gctgaaagag caacgcaaag caggcgagtt
     7261 gcgcgagttc ttgaacagac tctacaacgg cagcggtaag aaagaagatt tggatgccct
     7321 gactgatgaa gaaatcatcg aactggcctc caacctgcgc aaaggtgcat ctttcgcctc
     7381 tcctgtattc gacggtgcga aagagtctga aatccgcgaa atgctgaact tggcttatcc
     7441 gagcgacgat cctgaggttg aaaaactggg cttcaacgac agtaaaaccc aaatcacgct
     7501 gtatgacggc cgttcaggcg aagcatttga ccgcaaggtt acagtaggtg tgatgcacta
     7561 tctgaaactg caccacttgg ttgacgaaaa aatgcacgcc cgttctaccg gtccgtacag
     7621 tctggttacc cagcagcctt tgggcggtaa agcccagttc ggcggccaac gtttcggcga
     7681 gatggaggtt tgggcattgg aagcatacgg cgcggcatac acgctgcaag agatgttgac
     7741 tgtgaagtct gacgacgtga acggtcgtac caaaatgtac gaaaacatcg tcaaaggcga
     7801 acacaaaatc gatgccggta tgcccgagtc cttcaacgta ttggtcaaag agattcgctc
     7861 actgggcttg gatatcgatt tggaacgcta ctgatacggg tttcagacgg cataagggga
     7921 gctgttctgc aggtatgcgg ggcagccgac aatgtttaaa aacgaaatgc cgtctgaaaa
     7981 cactgtacct ctatccatat cgaaaatccg ccatgcggta aaaatacttc cttcaaggag
     8041 caaaaatgaa tttgttgaac ttatttaatc cgttgcaaac tgccggcatg gaagaagagt
     8101 ttgatgccat caaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg
     8161 aagtcaaaaa acctgaaacc atcaactacc gtacgttcaa acctgagcgt gacggtttgt
     8221 tctgtgccaa aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac
     8281 gcttgaaatt taaaggtgta acttgtgaaa aatgcggcgt ggaagtaacc ctgtccaaag
     8341 tgcgccgcga acgcatgggc catatcgaat tggctgcgcc cgtcgcacat atttggttct
     8401 taaaatccct gccttcccgc ttgggtatgg tgttagacat gactttgcgc gatatcgaac
     8461 gcgtattgta ctttgaagca tttgtggtaa ccgatcccgg tatgactccg ctgcaacgcc
     8521 gccaattgct gactgaagac gattactaca acaagctgga cgaatacggc gacgatttcg
     8581 atgccaaaat gggtgcggaa ggtatccgcg aattgctgcg taccctgaat gtagcgggcg
     8641 aaatcgaaat cctgcgccaa gagttggaat cgaccggttc cgacaccaaa atcaaaaaaa
     8701 tcgccaaacg cttgaaagta ttggaagctt tccatcgttc cggtatgaaa ctggaatgga
     8761 tgattatgga tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccattggatg
     8821 gtggtcgttt tgccacttcc gatttgaacg atttgtaccg ccgcgttatt aaccgtaaca
     8881 accgtctgaa acgtctgttg gaactgcatg ctcctgacat catcgtccgc aacgaaaaac
     8941 gtatgttgca agaagcagtt gactcgctgt tggataacgg ccgtcgcggt aaagccatga
     9001 ccggcgccaa caaacgcccg ctgaaatcat tggcagacat gattaaaggt aagggtggcc
     9061 gcttccgtca aaacctgctg ggcaaacgtg tggactactc cggccgttcc gtgattaccg
     9121 taggcccgta cctgcgtctg caccaatgcg gtttgccgaa aaaaatggct ttggaactgt
     9181 tcaaaccgtt cattttccac aaattggaaa aacaaggttt ggcctctacc gttaaagcag
     9241 cgaaaaaatt ggtagagcaa gaagtaccgg aagtatggga catcttggaa gaagtcatcc
     9301 gcgaacatcc gattatgctg aaccgtgcgc cgaccctgca ccgtttgggt attcaagcgt
     9361 tcgaacctat cttgattgaa ggtaaagcga ttcagttgca cccattggtg tgtgctgcgt
     9421 tcaacgccga ctttgacggc gaccaaatgg cggtacacgt tccattgagc ttggaagcac
     9481 aaatggaagc acgcacgctg atgctggctt caaacaacgt attgtctcct gccaacggcg
     9541 aaccgattat cgtaccttcc caagacatcg tattgggcct gtactacatg acccgcgacc
     9601 gtatcaatgc caaaggcgag ggcagcctgt ttgccgatgt gaaagaagtg catcgcgcat
     9661 accataccaa acaggttgaa ctgggtacga aaattaccgt acgtctgcgc gaatgggtga
     9721 aaaacgaagc aggtgagttt gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg
     9781 cattgttgag cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga
     9841 agaaaaaaga aatttctaaa ctgattaacg catcgttccg cctgtgcggc ttgcgcgata
     9901 cggttatctt tgctgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta
     9961 tttccattgc cgttgacgat atggaaattc caaaagaaaa agcggccttg ctggctgaag
    10021 ccaatgccga ggttaaagaa atcgaagacc aataccgtca aggtttggtt accaacggcg
    10081 aacgctacaa caaggtggtc gatatttggg gtcgtgccgg cgataagatt gctaaagcga
    10141 tgatggacaa cttgtccaaa caaaaagtta tcgaccgtga cggcaacgaa gtcgatcaag
    10201 agtcattcaa ctccatttat atgatggcgg actccggtgc ccgtggttct gcagctcaga
    10261 ttaaacagtt gtccggtatg cgtggcttga tggcaaaacc tgacggctcg attattgaaa
    10321 cgccgattac ctcaaacttc cgtgaaggtc tgaccgtatt gcaatacttt attgcgaccc
    10381 acggtgcgcg taagggtttg gcggataccg cattgaaaac cgcgaactcc ggttacctga
    10441 ctcgtcgtct ggtagacgta actcaagatt tggtcgttgt tgaagacgat tgcggtactt
    10501 cagacggctt tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg
    10561 atcgtatttt gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagt ggcgaaacct
    10621 tggttgaagc cggtacgttg ctgactgaaa aactggtgga tatgatcgac caatccggtg
    10681 tcgatgaagt caaagtccgt acgccgatta cttgtaaaac ccgtcacggc ctgtgtgcac
    10741 actgttacgg tcgtgacttg gcacgcggca aactggttaa cgccggtgag gcagtcggtg
    10801 tgattgctgc acaatccatt ggcgaaccgg gtacccagtt gaccatgcgt acgttccaca
    10861 tcggtggtgc ggcatcccgt gcggcagcag ccagccaagt ggaagccaaa tccaacggta
    10921 cggcacgatt cagcagccag atgcgctacg ttgccaacaa caaaggcgag ttggttgtca
    10981 tcggccgttc ttgtgaagtc gtgattcacg acgatatcgg ccgtgaacgc gaacgccaca
    11041 aagtacctta cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa
    11101 ccttggcaac ctgggatccg catacccgtc cgatgattac cgaacacgca ggtatggtga
    11161 aattcgaaaa cgtggaagag ggcgttaccg ttgccaaaca aaccgatgat gtaaccggtt
    11221 tgtccacttt ggtggtgatt gacggtaaac gtcgttcctc tagtgcttcc aaactgctgc
    11281 gtccgactgt gaaactcttg gacgaaaacg gcgtggaaat ctgtattccc ggtacgtcca
    11341 ctccggtttc gatggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa
    11401 tcggtaaagg cgacgtattg gcgcgtatcc cgcaagcctc ttccaaaacc cgcgacatta
    11461 ccggtggtct gccgcgcgtt gccgaattgt ttgaagcacg cgtgccgaaa gatgccggta
    11521 tgttggcgga aattaccggt accgtttcct tcggcaaaga gaccaaaggc aagcaacgtc
    11581 tgattgttac tgacgtggac ggtgtagcat acgagacctt gatttccaaa gagaaacaaa
    11641 ttctggtaca cgacggtcaa gtggtaaacc gcggtgaaac catcgtggac ggagctgttg
    11701 atccgcatga tattctgcgt ttgcaaggta tcgaagcact ggcacgctac attgtccaag
    11761 aggtgcaaga ggtttaccgt ctgcaaggtg tgaagatttc tgataaacac atcgaagtca
    11821 tcatccgtca aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggttcatta
    11881 ccggagagca ggtcgaacgc ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag
    11941 gcaaagagcc ggcacgttac gaaaacgtat tgctgggtat taccaaagcc tccctgtcca
    12001 ccgacagttt catttctgcc gcatcgttcc aagaaacgac ccgtgttctg accgaagccg
    12061 cgattatggg caaacaagac gaactgcgcg gtttaaaaga gaacgtcatc gtcggccgcc
    12121 tgattcctgc cggtaccggt ttgacttatc accgcagccg tcatcagcaa tggcaagggg
    12181 tagagcagga gactgctgaa acccaagtaa cggatgaata atcttcggcg cgtttattca
    12241 atacaaaacc gcaagcttta agcttgcggt ttttctttgt ccgattaagg caaaaacaag
    12301 cgttttcgtc attttgaggc gtgtggatta ttccttaggt attttcgggc cggagaccaa
    12361 cgaggtggcg ggtgtcgtcg gtacgtccgg agaccaaaat aactttgcca aggatgttgg
    12421 tttcggcggt caaaaaagta gcgtcttaat gttttccatt taaacaaatg tcgtctgaaa
    12481 cttcagacgg catttccttt aaaaaataaa tatgaaaccc agaaatctct tttttgcagg
    12541 ctgcctgctg acttcggcga cgtttgccga ggatatcggc gtacctgtcg aactgattaa
    12601 cgtcggtaat cggattgcga tgccgtctga aggggaaagc ctcgccctcc tgccgtttgc
    12661 cgaggatgta ccgccggttc gcgatgcaat gccgtctgaa gttcctaaaa gcgcggcagg
    12721 cggcgatgtt cggggtgacc ggatgggaat gccgattaac atcggatgag cgcggcttta
    12781 tggcataaaa aactgtcgtg gaaaggattt acaccccaaa taaatttccg ttacaacaag
    12841 atcaacagca atatgcccgc cttttattcg cgcagcagca aggaatggtt tgtcagtata
    12901 gaaaaaacgt attgacagta ttttctccag ccgtccgact gattgtgagg gatgtcggta
    12961 aatatttatc ggcaaacaag aaaatcatct ttcttcttgt cgttatgctt gactgtatac
    13021 ttgcaataaa aatataattc cactcttgcc gacatggtgt cggcaagtat ttaactcaac
    13081 aggacgagaa aatatgccaa ctatcaacca attggtacgc aaaggccgtc aaaagcccgt
    13141 gtacgtaaac aaagtgcccg cactggaagc ctgcccgcaa aaacgcggcg tgtgcacccg
    13201 tgtatacacg actaccccta aaaaacctaa ctctgcattg cgtaaagtat gtaaagtccg
    13261 cctgaccaac ggttttgaag tcatttcata catcggcggc gaaggccaca acctgcaaga
    13321 gcacagtgtc gtattgattc gcggcggtcg tgtaaaagac ttgccaggtg tgcgttacca
    13381 cactgtacgc ggttctttgg atactgcagg tgttaaagac cgtaaacaag cccgttccaa
    13441 atacggtgct aagcgtccta aataattact gggacttaaa taggcacgtc ggccgcctaa
    13501 gctgaacaac ggccgagtaa gtgaatactc aattgggtat tcatgggaat agacccgact
    13561 gaatagatta aaggaaatta aaatgccaag acgtagagaa gtccccaagc gcgacgtact
    13621 gccagatcct aaattcggca gcgtcgagtt gaccaaattc atgaacgtat tgatgattga
    13681 cggtaaaaaa tccgttgccg agcgtatcgt ttacggtgcg ttggaacaga ttgagaaaaa
    13741 aaccggcaaa gtagcaatcg aagtatttaa cgaagccatt gcaaacgcca aacctatcgt
    13801 ggaagtgaaa agccgccgtg taggtggtgc aaactaccaa gttcctgttg aagttcgtcc
    13861 ttcacgccgt ttggctttgg caatgcgctg ggttcgcgat gcggcccgca aacgtggtga
    13921 gaaatccatg gacctgcgtt tggcaggcga attgattgat gcgtccgaag gccgtggcgg
    13981 tgcgttgaaa aaacgtgaag aagtacaccg tatggctgaa gccaacaaag cattctctca
    14041 cttccgtttc taattttgaa aggctaataa aatggctcgt aagaccccga tcagcctgta
    14101 ccgtaacatc ggtatttccg cccatatcga tgcgggtaaa accacgacga cagaacgtat
    14161 tttgttctat accggtttga cccacaagct gggcgaagtg catgacggtg cggctactac
    14221 cgactacatg gaacaagagc aagagcgcgg tattaccatt acctccgctg ccgttacttc
    14281 ctactggtcc ggtatggcga aacaattccc cgagcaccgc ttcaacatca tcgacacccc
    14341 gggacacgtt gactttaccg tagaggtaga gcgttctatg cgtgtattgg acggcgcggt
    14401 aatggtttac tgcgcggtgg gcggtgttca accccaatct gaaaccgtat ggcggcaagc
    14461 caacaaatac caagtgccgc gcttggcgtt tgtcaataaa atggaccgtc agggtgccaa
    14521 cttcttccgc gttgtcgagc aaatgaaaat ccgtttgcgc gcaaaccctg tacctatcgt
    14581 cattccggtt ggtgcggaag acaacttcag cggtgtggtt gatttgttga aaatgaaatc
    14641 catcatttgg aatgaagccg ataaaggtac aacctttacc tatggcgata ttcctgccga
    14701 attggtcgaa actgccgaag aatggcgtca aaatatgatt gaagccgcag ccgaagccag
    14761 cgaagaactg atggacaaat acttaggcgg cgacgagctg accgaagaag aaatcgtagg
    14821 cgcgttgcgt caacgtactt tggcaggcga aattcagcct atgctgtgtg gttctgcatt
    14881 taaaaacaaa ggtgttcaac gtatgttgga cgcagttgta gaattgctgc cagctcctac
    14941 cgatattcct ccggttcaag gtgtcaaccc gaataccgag gaagccgaca gccgtcaagc
    15001 cagcgatgaa gagaaattct ctgcattggc gttcaaaatg ttgaacgaca aatacgtcgg
    15061 tcagctgacc tttatccgcg tttactcagg cgtagtaaaa tccggcgata ccgtattgaa
    15121 ctccgtaaaa ggcactcgcg aacgtatcgg tcgtttggta caaatgactg ccgcagaccg
    15181 tactgaaatc gaagaagtac gcgccggcga catcgcagcc gctattggtc tgaaagacgt
    15241 tactaccggt gaaaccttgt gtgcggaaag cgcgccgatt atcttggaac gtatggaatt
    15301 ccccgagccg gtaatccata ttgccgttga gccgaaaacc aaagccgacc aagagaaaat
    15361 gggtatcgcc ctgaaccgct tggctaaaga agacccttct ttccgtgtcc gtacagacga
    15421 agaatccggt caaaccatta tttccggtat gggtgagctg cacttggaaa ttattgttga
    15481 ccgtatgaaa cgcgaattcg gtgtggaagc aaatatcggt gcgcctcaag tggcttaccg
    15541 tgaaactatc cgcaaagccg ttaaagccga atacaaacat gcaaaacaat ccggtggtaa
    15601 aggtcaatac ggtcacgttg tgattgaaat ggaacctatg gaaccgggtg gtgaaggtta
    15661 cgagtttatc gatgaaatta aaggtggtgt gattcctcgc gaatttattc cgtctgtcga
    15721 taaaggtatc cgcgatacgt tgcctaacgg tatcgttgcc ggctatcctg tagttgacgt
    15781 acgtatccgt ctggtattcg gttcttacca tgatgtcgac tcttcccaat tggcatttga
    15841 attggctgct tctcaagcgt ttaaagaagg tatgcgtcaa gcatctcccg ccctgcttga
    15901 gccgattatg gcagttgaag tggaaacccc ggaagaatac atgggcgacg taatgggcga
    15961 cttgaaccgc cgtcgcggtg ttgtattggg tatggatgat gacggtatcg gcggtaaaaa
    16021 agtccgtgcc gaagtacctc tggcagaaat gttcggttat tcgaccgacc tgcgttctgc
    16081 aacccaaggc cgcgctactt actctatgga gttcaagaaa tattctgaag ctcctgccca
    16141 catagctgct gctgtaactg aagcccgtaa aggctaatca ggcaaatagg ccgtccgaaa
    16201 ggctgaaatg atttttcaga cggcattgtt ctttaatcga tctttaatgt aaaggaatta
    16261 gc
//
LOCUS       DAWKOD010000033        16118 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000033 DAWKOD010000000
VERSION     DAWKOD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.033"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(345..767)
                     /locus_tag="WJK73_000789"
     CDS             complement(345..767)
                     /locus_tag="WJK73_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ7557303.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(885..1556)
                     /locus_tag="WJK73_000790"
     CDS             complement(885..1556)
                     /locus_tag="WJK73_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ7557304.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1572..2198)
                     /locus_tag="WJK73_000791"
     CDS             complement(1572..2198)
                     /locus_tag="WJK73_000791"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ7557305.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2305..3123)
                     /locus_tag="WJK73_000792"
     CDS             complement(2305..3123)
                     /locus_tag="WJK73_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7557306.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3382..3822)
                     /locus_tag="WJK73_000793"
     CDS             complement(3382..3822)
                     /locus_tag="WJK73_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ7557307.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3885..5291)
                     /locus_tag="WJK73_000794"
     CDS             complement(3885..5291)
                     /locus_tag="WJK73_000794"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ7557308.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFAEAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5461..6576
                     /gene="lptF"
                     /locus_tag="WJK73_000795"
     CDS             5461..6576
                     /gene="lptF"
                     /locus_tag="WJK73_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ7557309.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLDLRNGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6573..7643
                     /gene="lptG"
                     /locus_tag="WJK73_000796"
     CDS             6573..7643
                     /gene="lptG"
                     /locus_tag="WJK73_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ7557310.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGLIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7953..10538
                     /gene="acnB"
                     /locus_tag="WJK73_000797"
     CDS             7953..10538
                     /gene="acnB"
                     /locus_tag="WJK73_000797"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ7557311.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10680..11675
                     /locus_tag="WJK73_000798"
     CDS             10680..11675
                     /locus_tag="WJK73_000798"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ7557312.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTTFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11744..11845)
                     /locus_tag="WJK73_000799"
                     /pseudo
     CDS             complement(11744..11845)
                     /locus_tag="WJK73_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11899..12912)
                     /gene="ilvC"
                     /locus_tag="WJK73_000800"
     CDS             complement(11899..12912)
                     /gene="ilvC"
                     /locus_tag="WJK73_000800"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ7557313.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12992..13285)
                     /locus_tag="WJK73_000801"
     CDS             complement(12992..13285)
                     /locus_tag="WJK73_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ7557314.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(13352..13843)
                     /gene="ilvN"
                     /locus_tag="WJK73_000802"
     CDS             complement(13352..13843)
                     /gene="ilvN"
                     /locus_tag="WJK73_000802"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ7557315.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13854..15581)
                     /gene="ilvB"
                     /locus_tag="WJK73_000803"
     CDS             complement(13854..15581)
                     /gene="ilvB"
                     /locus_tag="WJK73_000803"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ7557316.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         3780 a         4384 c         4083 g         3871 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccact ataattcaat aaattaatat atggcttaaa
       61 ataacgggat tctcgcctcc cgcccgcccg cagaagcagg cgggtatcat tttaaaacgc
      121 ggcatttaaa atttgaccga aaattgttga caatccggaa tcaagtctgc acaatacccc
      181 gacaagtcca agtattataa aggctgaata aagaggaaac agcaggcaga tatattcggg
      241 aggtgcagtc cgaatatatc tgctttttta tgcgcctccg gattgcctgc cgcacctttc
      301 ccttcagacg gcatcagccg tttccccata atgccgcccg atgcctattt atctgccccg
      361 gcaatttcaa aactgtgggt aatctttgcc gctttgccca acataatcga agccgaacag
      421 tatttttcgg cagacatctg aacggcgcgc tcaatggccg attctttcaa atcatgcccg
      481 aatactttga aatggatgtg gatttcggta aacacgcgcg gcgcatcgtc cgcccgtttc
      541 gccgtaaccg tcgcacggca gtcagtcact ttctgacgct gtttttcggc aatcatcacc
      601 acatcgatgc tcgaacagcc cgccacgccc aacagcagca tttccaaagg gctgggcccg
      661 cgcttggccg caccttctgc cgccgacccc tccataacga cgctgtgccc gccttccgtt
      721 gttccgacaa agcacattcc gtctatccat tttgatgtaa cctgcatggt gtcattcctg
      781 aaaatagcgt taaaaccgct ttgcatatgg cgttattgta aacaatttca agcggcttat
      841 gcagaaatat ggacaaaacg gcaaaaaaac gcctgaaaat cgatttacgg tttggctgcc
      901 tggccgttga tctgcaccga tttgagtttc agcgtatagg ttttgccgtc gtcggtatag
      961 ccgatttgtg ccggaatatt gttcagggac ggtgcgaaga aatacattac cgcatcgtcg
     1021 ccgcgccgca cccgatattt gacgacttcg gtttccacgc cgcctatgct gtattttcct
     1081 gtacccgcct tattcaaacc gccgacggaa taaagttttt tgccgttggt gattttcagc
     1141 cccgggggga gtttcgcgtc atttgccgcc aactgccagg caagcgtgaa caaatccata
     1201 gccttggggc tttgctcggt tttgctctcg cccgctttgc cgtaggttac gctgccgtcg
     1261 gcgaatttgg cttccgcata cagtttgccc ctgcgtatgt ctctatagta ggtagggtgc
     1321 agggtattgc cgacaaccgt accgccggac tcgaaacgga tattgtatag cggcacttta
     1381 atcgtcgaaa cgattttgta agcattgccg ctgcgttcaa atgtcatcgt ggcgggaatg
     1441 ccgtagctgc cggaatagtg cagcacggcg gattggggca gccctgccgc atacgcgcac
     1501 ggcagggcgg cggacaaaat ggcggcggaa aatatatttt taaaagtctt catcatttgc
     1561 tcccgcccgg tttacgccgt caggaaacgg gcggcatcgg cgttttccga atttctgacg
     1621 cggtttccct cgataatcag gcggccggcg gcaaaatcgg caacggcttt cggataaagt
     1681 ttatgctcaa cagccaaaac ccgggcggca acatcgtctg ccgtatcgcc gtcgagtatc
     1741 ggcacaatcc cttgcgatac aatcgggccg caatccagtt cggcagtaac gaaatggatg
     1801 gtgcagccgg caacgcggca gcccgcctcc aaagcgcgtt cgtgcgtatg aagtccggta
     1861 aacgagggca ggacggacgg gtgaatgttc atcagcctgc cttcgtaatg ggcacagaac
     1921 tcaggggtca gaatccgcat aaaacctgcc aaaaccacca agtcgggttg atatgcgtcg
     1981 attttttcca tcatggcggt atcgaaggca agtcgtgatt caaagttttt atgattcagg
     2041 ctgtcggtcg gaataccctg accggccgcc cattgcaaac cggcagccgt ttcgctgttg
     2101 ctcaacacgg cggcaatacg gacgttggga atggcggcat tgacgattgc ctgcatattg
     2161 ctgccgcgtc cggaaatcag gatgacgatg tttttcataa tggtgcgctt ttgaaaggga
     2221 tgccatctga accgctgttt ggtggtttca gacggcattt gccgtaaaaa tgcccgaaaa
     2281 cctgtttcgg gcatggattc ggacttaatt tacttttttg atgtcgactt gagccggctg
     2341 cttggcgggc gcgttttcgg gtgcgccgat tttgaccagt ttcacatcaa ataccaaagt
     2401 ggcgttcggg ccgattttgt cgcccgcacc ctgttcgcgg taggcaaggt tggacgggat
     2461 gtagaacgtg gcttcgccgc cttctttcag aagctgtacg ccttcggtcc aacccggaat
     2521 cacttggctc aaagggaagg tgaccgggcc gccgttggct ttgctgctgt cgaataccgt
     2581 accgtcaatc aggcggcctt cgtattccac ggtaacgatg tcgtctttgg tcggctgttt
     2641 gccttcgccc tgtttggtga ttttgtattg caggccggaa gcagtggtct tcacgccgtc
     2701 tttggcggca ttttctttca gaaaggcttc gcctttttct ttattggcct tcgcgtccgc
     2761 cttgtgtttt tctacggctt tagcctgttg ttcctgaagg aatttcatca tgacttcctg
     2821 agcctgctct tcggtcattt tgatttcttt gccgtcatac actgcctgca tggcttcggt
     2881 aaagactttc aaatcgattt ccgcgccctg ttccttcatt tgcttcaggg agcgtccgat
     2941 gtccacgccc atcgcatagc ttgcctgctg catcgtgctg ccgatcgaag aggtgtcgcc
     3001 ctgcgcggaa gaagcggcgg caggttcgga tgcagatgcg ggggcggctt cttttttgcc
     3061 gcaggcggaa agtgccaaag cggcggaaag ggtcagtgcg ctgattttga aaatggtgtt
     3121 catgatggat cttcgctgtc gataaggtcg gaaaaacggg attatagccg agtttgaatg
     3181 tttcaacaca caggatgaca cataaagcgt caatcgtgtg ttgccctgtt ttggaaaaga
     3241 ttgaaccttc caaaataagt tttgattcta ccgccccgag ggacagatgt ccaagtggcg
     3301 gggttcaacc gataaggaaa ttttaatcaa atagaatcaa gcctgtttaa attttgtaaa
     3361 tgcggcattt cagacggcat tttatgcctt gccctccatg ccgtgatgtt cgatggcaaa
     3421 accgcttcgg cggtaggcgg taaagcgttc gcgcgcgtcg gcgagctctt ccaggctgtt
     3481 gccgacgatt tctaaaacgc gcgcgggcaa gaccggagcg gtgttccaaa aaccgtcgga
     3541 caggttcaaa acggccatgc cttcgggaat tcggggcaga ttgccgccgc aggcaagcag
     3601 gacggatggt tcagacggca tggcttcttc cgtttcccaa atttcgtgcg gaataaaact
     3661 ctcggcctcg tattgccaaa gcattttgtc caattcctga agctgcccga acgaatcgga
     3721 ccacaccagt atcctgccgc cgtcccgaat cgcacgggca atcaggcggc aggtgaaaat
     3781 cggaacttgg gcaacgtgcg tgtaaaaggt ggctttcggc atattgtttg agcatttggc
     3841 aggacgatgc cgtctgaaag gcttcagacg gcattgtggc aaaattaaag attccgcaga
     3901 tagttcagca gcaagggaac gggacggcca gtcgcacctt tttccgctcc ggatttccac
     3961 gccgtacccg cgatgtcaag gtgcgcccac gggtagcctt cggtgaagta agacaggaat
     4021 gttgcggcgg taatcgtgcc cgcgccgggc gtgccgatat tggggatgtc ggcaaagttg
     4081 gatttgagct ggtctttgta ggtttcaaag agcggcagtt gccacgcttt gtcgtccacg
     4141 ttgtaggaag cggcaagcag gctgtcgacc aaatcttgat tgttgcccat cacgccgctg
     4201 acatcgtgcc ccaaggcgac aatacacgcg ccggtcaggg tggcgacgtc gatgacggct
     4261 ttgggtttga actgttcggc gtaagtgagc gcgtcgcaca aaatcagacg gccttcggca
     4321 tcggtgttca acacttcgat ggtcagacct ttcatacttt tcacgacatc gcccggtttg
     4381 tttgccgcgc cggaaggcat attttcacaa gtggcgacga cggcaatcag gttaatcggc
     4441 agttgcagtt tgacggcggc gcagaaggtg ctgatgacgg ttgccgcgcc gcacatatca
     4501 aacttcattt cgtccatgtt caggccgggt ttgagggaaa tgccgccggt gtcgaaggta
     4561 atgcctttgc cgaccaatac cacaggcgcg gcttctttgt ccgccgcacc gaaatagctc
     4621 agttcgacca aatagggatc ttcgacactg cctttggcga ccgaccaaaa cgaacccatg
     4681 ttttctttga tgtagtcttt ttcaatgatt ttggcgtgtg cgcccaattt ttcggcttcg
     4741 gctttggcgg tgcgcgctaa aaattcgggc gtgcattcgt tgggcgcggc gttgcccaag
     4801 tcgcggcaga ggctttgtcc gtaaacctgc gcttcggcaa cgcgcagggc ttctttgacg
     4861 gcggcttcgt gtgcgctgtg gaacacggct tctgcaaatt tggcgggctt ggcttctttt
     4921 ttatagcggt cgaaacggta ggcggcattg ccgaaagcaa tcgcaaacgc ttcggcaacg
     4981 gctgcggctt gcgcttcttc aaaggcatga acgtccacat tgaccgtttc ctgattttgc
     5041 gcccatttgg cggcttcggc agcggccttg ttcaatgcgg cgcggccggt gcttttcaga
     5101 cagcatacgg caacagcctg caaaccgttg cctgtcggga tttttgtgtc ggcaaaattt
     5161 tgaccttctt caagcgaaga caaaagggca agggcggtcg gatggtttaa ttgcgcctct
     5221 tcggtgcaga caaataactg tgcgcctgcc tgctgttcct gcaagatttc ggtttttgtg
     5281 ctaaattcca cgtttattct cctgattgag acggttgtcg gtagttttcg gacggccttt
     5341 cgctcaaaag accgtctgaa gacggctggc acgattgtac cccatttgaa gcaccgtctg
     5401 aaaccttgcg cggacaatcc gcctgcgccg aaccgcttac cgccccctga ccgcgattct
     5461 atgatttatc aaagaaacct catcaaagaa ctctctttta ccgccgtcgg cattttcgtc
     5521 gtcctcttgg cggtattggt ctccacgcag gcaatcaacc tgctcggccg tgccgccgac
     5581 gggcgtgtcg ccatcgatgc cgtgttggca ttggtcggct tctgggtcat cggtatgacg
     5641 ccgcttttgc tggtgttgac cgcatttatc agtacgttga ccgtgttgac ccgctactgg
     5701 cgcgacagcg aaatgtcggt ctggctatcc tgcggattgg cattgaaaca atggatacgc
     5761 ccggtgatgc agtttgccgt gccgtttgcc gttttggttg ccgtcatgca gctttgggtg
     5821 ataccgtggg cagagctacg cagccgcgaa tacgctgaaa tcctgaagca gaagcaggaa
     5881 ttgtctttgg tggaggcagg cgggttcaac agcttgggca agcgcaacgg cagggtttat
     5941 tttgtcgaaa ccttcgacac tgaatccggc attatgaaaa acctgttcct gcgcgaacag
     6001 gacaaaaacg gcggcgacaa cattatcttc gccaaagaag gcaatttctc gctgaacgac
     6061 aacaaacgca cgctcgacct gcgcaacggc taccgttaca gcggtacgcc tggacgcgcc
     6121 gactacaatc aggtttcctt ccaaaaactc aacctgatta tcagcaccac gcccaaactc
     6181 atcgaccccg tttcccaccg ccgtaccatt ccgaccgccc aactgattgg cagcagcaac
     6241 ccgcaacatc aggcggaatt gatgtggcgc atctcgctga ccgtcagcgt cctcctactc
     6301 tgcctgcttg ccgtgccgct ttcctatttc aacccgcgca gcggacatac ctacaatatc
     6361 ttgattgcca tcggtttgtt tttaatttac caaaacgggc tgaccctgct ttttgaagcc
     6421 gtggaagacg gcaaaatcca tttttggctc ggactgctgc ctatgcacat catcatgttc
     6481 gtcatcgcaa tcgtacttct gcgcgtccgc agcatgccca gccagccctt ctggcaggct
     6541 gttggcaaaa gtctgacatt gaaaggcgga aaatgaacct gatttcacgt tacatcatcc
     6601 gtcaaatggc ggttatggcg gtttacgcgc tccttgcctt cctcgctttg tacagctttt
     6661 ttgaaatcct gtacgaaacc ggcaacctcg gcaaaggcag ttacggcata tgggaaatgc
     6721 tgggctacac cgccctcaaa atgcccgccc gcgcctacga actgatgccc ctcgccgtcc
     6781 ttatcggcgg actggtctct ctcagccagc ttgccgccgg cagcgaactg accgtcatca
     6841 aagccagcgg catgagcacc aaaaagctgc tgttgattct gtcgcagttc ggtttgattt
     6901 tcgccattgc caccgtcgcg ctcggcgaat gggttgcccc cacgctgagc caaaaagccg
     6961 aaaacatcaa agccgccgct attaacggca aaatcagcac cggcaatacc ggcatttggc
     7021 tcaaagaaaa aaacagcatt atcaacgtgc gcgaaatgtt gcccgaccat accctgctgg
     7081 gcattaaaat ctgggcccgc aacgataaaa acgaactggc agaggcagtg gaagccgatt
     7141 ccgccgtttt gaacagcgac ggcagttggc agttgaaaaa catccgccgc agcacgcttg
     7201 gcgaagacaa agtcgaggtc tctattgcgg ctgaagaaaa ctggccgatt tccgtcaaac
     7261 gcaacctgat ggacgtattg ctcgtcaaac ccgaccaaat gtccgtcggc gaactgacca
     7321 cctacatccg ccacctccaa aacaacagcc aaaacacccg aatctacgcc atcgcatggt
     7381 ggcgcaaatt ggtttacccc gccgcagcct gggtgatggc gctcgtcgcc tttgccttta
     7441 ccccgcaaac cacccgccac ggcaatatgg gcttaaaact cttcggcggc atctgtctcg
     7501 gattgctgtt ccaccttgcc ggacggctct tcgggtttac cagccaactc tacggcatcc
     7561 cgcccttcct cgccggcgca ctacctacca tagccttcgc cttgctcgcc gtttggctga
     7621 tacgcaaaca ggaaaaacgc taaaccaatg ccgtctgacc cccttcggac ggcattggtt
     7681 tttattatca tattcctgta gacagatagt cgttccctat tatagtggat taacaaaaac
     7741 cagtacggcg ttgcctcgcc ttgccgtact attgtactgt ctgcggcttc gtcgccttgt
     7801 cctgattttt gttaatccac tatacattac ctgtcataac agttccattt ttgttaaaac
     7861 tagtctatga tagcggtaca aatattgttt acaatattta acgcaaatca tttgcaaccc
     7921 gacaaaagaa aaacagaaaa aggaacaaag agatgttaga agcctatcgt aaagccgccg
     7981 ccgagcgcgc cgccctcggc attcccgccc tccctttgaa cgcgcagcaa accgccgatt
     8041 tggttgagct gctgaaaagc ccgcccgcag gcgaaggcga gttcttggtc gaactgcttg
     8101 cccaccgtgt tccgcccggt gtggacgatg ccgccaaagt caaagcctca ttcctggctg
     8161 ccgttgccga aggcagcgcg tccagcccgc tgatctcccc cgaatatgcg accgaactct
     8221 taggtacgat gctcggcggt tacaatatcc acgccttaat cgaactcttg gacgacgaca
     8281 aactcgcgcc cattgccgcc aaaggcttga aacatacgct tctgatgttc gattccttcc
     8341 acgacgttca ggaaaaagcc gaaaaaggca acaaatacgc gcaagaagtt ttgcaatctt
     8401 gggcagatgc cgaatggttt acctcacgcg ccaaagttcc cgaaaaaatc accgttaccg
     8461 ttttcaaagt tgacggcgaa accaatacag acgacctctc ccccgcgccc gacgcgtgga
     8521 gtcgtcccga tattccgctg cacgcgctgg ccatgctgaa aaacccgcgc gacggcatca
     8581 cgcccgacaa accgggcgaa gtcggtccga ttaaattgtt ggaagaactc aaagccaaag
     8641 gccatccggt tgcttacgtc ggcgacgtgg tcggtaccgg ttcttcacgc aaatccgcga
     8701 ccaactccgt catttggcat accggcgaag acattccgtt cgtgccgaac aaacgcttcg
     8761 gcggcgtatg tttgggcggc aaaatcgcgc cgattttctt caatacccaa gaagattccg
     8821 gcgcgctgcc gattgaagtc gatgtatctg ctctaaaaat gggtgatgtc gtcgatatcc
     8881 tgccttatga aggcaaaatc gtgaaaaacg gcgagactgt tgccgagttt gaattgaaat
     8941 cacaagtatt gctggacgaa gtgcaagccg gcggccgtat caacctgatt atcggccgag
     9001 gtctgaccgc caaagcgcgc gaagccctga aactgcctgc ctctactgca ttccgcctgc
     9061 cgcaagcgcc tgccgaaagc aaagccggtt tcaccttggc gcaaaaaatg gtcggccgcg
     9121 cctgcggtct gcccgaagga caaggcgtgc gcccgggtac ttactgcgaa ccgcgtatga
     9181 cgacggtcgg ctcgcaagac acgaccggcc cgatgacccg cgacgagttg aaagacttgg
     9241 cttgtttggg cttctccgcc gatatggtga tgcagtcttt ctgccacacc gccgcctatc
     9301 cgaaacctgt cgatgtaaaa acccataaag aactgcccgc ctttatttcc acccgtggcg
     9361 gcgtgtcact gcgtccgggc gacggcgtca tccactcgtg gctcaaccgc ctgctgctgc
     9421 ccgataccgt cggcaccggc ggcgacagcc atacccgttt ccccatcggt atttccttcc
     9481 ccgccggctc cggcttggtt gcctttgccg ccgcaacggg cgtaatgccg ctcgatatgc
     9541 ccgagtctgt attggtacgc ttcagcggca agctgcaacc gggcgtaacc ctgcgcgatt
     9601 tggtgaacgc catcccgctg tacgcaatca aacaaggttt gctgaccgtt gccaaagccg
     9661 gtaagaaaaa catcttctcc ggccgcatcc tcgaaatcga aggcctgcct gatttgaaag
     9721 tggaacaagc ctttgaattg accgacgcat ccgccgaacg ctccgccgcc ggctgtaccg
     9781 tgaagctcaa caaagagccg attatcgagt acatgaaatc caacgtcgtg ttgatgaaaa
     9841 acatgattgc caacggctat caagacccgc gcactttgga acgccgcatc aaagctatgg
     9901 aaaaatggct ggcaaatccc gagttgctcg aagcggataa agatgccgaa tacgccgccg
     9961 tgattgaaat caacatggac gacatcaaag agccgattat cgcctgcccg aacgacccgg
    10021 acgacgtgtg cttcatgtcc gaacgctccg gcaccaaaat cgacgaagta ttcatcggtt
    10081 cgtgtatgac caacatcggc cacttccgcg ccgcctccaa acttttggaa ggcaaggcag
    10141 acacccccgt ccgcctgtgg attgcgccgc cgaccaaaat ggacgcgaaa caattgtccg
    10201 acgaaggaca ctacggcgta ctcggacgtg ccggcgcgcg tatggaaatg ccgggttgct
    10261 ccttatgtat gggtaatcag gcgcaagtac gcgaaggtgc gaccgttatg tccacctcca
    10321 cccgcaactt cccgaaccgt ttgggtaaaa acacctttgt ttacctcggt tcggcggaat
    10381 tggcagcgat ttgctccaaa ctgggtaaaa tcccgaccgt tgaagaatat caagccaata
    10441 tcggcatcat caacgaacag ggcgataaaa tctaccgcta tatgaacttc aacgaaatcg
    10501 acagctacaa cgaagtagcc gagaccgtga acgtttaatc cccgtcatcc gtatgaagta
    10561 agggattgac cgcaatgccg tctgaacaac cttcagacgg cattgcaaca ttccgctaac
    10621 ccttctttcc gcaaacgctg caaatacggc gttcacgccc ccacataaag gaaacgacag
    10681 tgaacctgaa aaaccgccat tttctgaaac ttttagactt cacgccggaa gaaatcaccg
    10741 cctacctcga ccttgccgcc gaattgaaag ccgccaaaaa agcagggcgc gagattcagc
    10801 ggatgaaagg gaaaaacatc gccctgattt ttgaaaaaac ctctactcgg acgcgctgcg
    10861 cgtttgaagt cgccgcgcgc gatcaaggcg cgggagtgac ttatttagag ccgtccgcca
    10921 gccaaatcgg gcataaggaa agcatcaaag acaccgcccg cgtgttgggc aggatgtacg
    10981 atgccatcga atatcgcggt ttcggtcagg aaactgtcga agaattggcg aaatacgcgg
    11041 gcgtacccgt gttcaacggg ctgaccaacg agttccatcc cacacaaatg cttgccgacg
    11101 cactgactat gcgcgaacac agcggcaaac ctttgaacca aaccacgttt gcctacgtcg
    11161 gcgacgcgcg ttacaacatg ggcaattccc tgctgatttt aggggcaaaa ttggggatgg
    11221 acgtgcgtat cggcgcaccg caaagcctgt ggccgtctga aggcattatt gccgccgcac
    11281 acgccgccgc caaagaaacc ggcgcaaaaa ttaccctgac cgaaaacgcg catgaagccg
    11341 tgaagaatgt tgattttatt cataccgatg tgtgggtcag catgggcgag ccgaaagaag
    11401 tctggcagga acgcatcgat ttgctgaaag attaccgcgt tacgcccgaa ctgatggcgg
    11461 catcgggcaa tccgcaagtc aaattcatgc actgcctgcc cgccttccac aaccgcgaaa
    11521 ccaaagtcgg cgaatggatt tacgaaacct tcgggctgaa cggtgtggaa gttacagaag
    11581 aagtattcga aagccccgcc agcatcgtgt tcgatcaggc ggaaaaccgt atgcacacga
    11641 ttaaagcggt aatggtcgcg gctctgggcg actgacagaa ctgtgcctgt ttaaattcat
    11701 ccgcaacaca gataccgtct gaacacgatg ttcagacggt atccatatat agtggattaa
    11761 atttaaacca gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcagcttcg
    11821 tcgccttgtc ctgatttttg ttaatccact ataaaaaaac tgcctacacg atgtgtaggc
    11881 agtcccgttt gaaaacaatc agtttttgtc ttggtcaacc aatttgttgg cagtaatcca
    11941 aggcatcatg gcacgcagtt gtgcgccgac tttttcaact tggtggtcgg cattcaaacg
    12001 gcggcgggca gtcatagacg cgtagttgac gttaccctct tggataaaca tttttgcgta
    12061 ttcgccggtt tggatgcgtt tcagagcatt gcgcatggct tctttgctgg aagcattgac
    12121 cacttcaggg ccggtaacgt attcgccgta ctccgcattg ttggaaatgg agtagttcat
    12181 attggcaata ccgccttcga aaatcaggtc aacaatcagt ttcatttcgt gcagacattc
    12241 gaagtaagcc atttcaggcg cgtaaccggc ttcggtcagg gtttcaaaac ctgctttgat
    12301 caactcgacc acgccaccgc acaatacagc ttgttcgccg aacagatcgg tttcggtttc
    12361 ttcgcggaaa gtggtttcaa tcacaccgcc tttggtgccg ccgttggcag ccgcataaga
    12421 cagggcgatg tctttggcct tgccggaatt gtcttggtaa acggcaatca gagaaggcac
    12481 gccgccgccg cgtttgtatt cactgcgtac ggtatgaccc gggcctttgg gggcaaccat
    12541 aatcacgtcc aagtcggcac gcggaacgat ttggttgtag tgaacgttga agccgtgtgc
    12601 aaatgccagc gttgcgcctt ctttcagatt ggctgcaact tcggcgtgat agacggcagg
    12661 catggtttcg tcaggcagca gcagcataac gacatcggct tctttggtcg cttcagcaac
    12721 agttttgacg acatggccgg ctgcttcggc ttttttccaa gaagaacctt ggcgcagacc
    12781 aatcaccacg tttacacccg aatctttcag gttggcagca tgggcatggc cttgcgaacc
    12841 gtaaccgatg atggcaacgg ttttgccttt gatcagggac agatcggcat ctttatcgta
    12901 atagacttgc atttgatttc ctttaaggta aatggttgtc gaagccttaa aatgttgagc
    12961 ggcttcggac gggttaaaca gagtgtgccg cttaagcggc aacttcattc atcaatacga
    13021 tttccaacgc ttcggttttg ccgtcgacgg actggacgaa ggcttggaaa tgcgcgctgg
    13081 cattgtgttc gtcaatagct gcttgggatt tccaattttc cacgaaaaca aaacggttcg
    13141 gtttgccgat ttcctgatgg agatcatagc tgatgttgcc ctcttccgca cggctggctt
    13201 tgaccagttc tttaaactgt gctgccagtg tttctgtgta ttccggttta acggtaacca
    13261 gtgcgacaat tttaatgttc gacataaatc tctcctgccg ttcgtttttc agacggcatt
    13321 caaataccgt gccgtctgaa aggttacggc gttaaatttt caaaatacgc tcaccgcgac
    13381 cgatgccggc cgcgcctgta cgtacggttt ccaaaatttg ggcgcgtccg accgtttcca
    13441 aaaaggaatc cagcttgtct gtcgagccgg taatttcaat cgtatagctg cggtcggtta
    13501 cgtcgatgat gctgccccgg tagatttcgg tcaagcgtaa aaattcgtcg cggtctttgc
    13561 cggcggcacg gacttttacc aacatcagtt cgcgttcgac aaaacggctt tcgttcaaat
    13621 cgaccacttt aatcacttca atcaatttat tgagttgctt ggtgatttgt tcgataacct
    13681 gctcgtcgcc gtgtgtaacg atggtcatac gggacagggt tttgtcttcg gtcggcgcaa
    13741 ccgccaaaga atcgatattg taatcgcgtg cagagaacaa accgaccacg cggctcatcg
    13801 cacctgattc gttttcgatc agaacagata agatatgtcg catttgcctc tccttacgcc
    13861 tttccgtccg cacgcatatg cggcggaagt accatttcgt ccaaaccttt gccgttgccg
    13921 accatgggca tcacattctg tttctggtcg gtcaggaagt cgataaacac cagcctgtct
    13981 ttttggttca atgcttccaa caacgcacct tccacatcag acttcttgtc cacgcggata
    14041 ccgatatggc cgtatgcctc ggcaagtttg acgaaatcgg gcaaagaatc gaaataggtt
    14101 tccgactctc gtccgccgta atatatttcc tgccactggc gtaccatacc gagataaccg
    14161 ttgttcagcg taatgacgtt aaccggaatc cgatattgga aacaggtgga cagctcttgg
    14221 atgttcatct ggatcgagcc gtcgccggta atacagaata cgtcttgatc cggggcggca
    14281 agttttgcac caatcgcata aggcagacca acgcccatcg tacccaaacc gccggagttg
    14341 agccattggc gcggacgttc gaagggataa tattgagccg caaacatttg atgctgccct
    14401 acatccgatg tgatgattgc cgaattgccg gtaatctcgg caagcttctg aatcacatat
    14461 tgtggcttga taatttcgct gccgttgtca aaccacaagc aatcgcggga acgccattcc
    14521 tctatggttt tccaccattt gcccaaagca tcttcagacg gcacggactc ttgtttttgc
    14581 cacagcgcaa ccatctcgga caaaacgttt ttcacgtcgc cgacaatcgg aatgtccgcc
    14641 ttcacgcgtt tggcgatgct ggaaggatcg acatcgatat ggataacctt cttcgccttc
    14701 tcgaaaaatt tggacggtac ggaaaccaca cggtcgtcaa aacgcgcacc tacggcaaga
    14761 acgacatccg cattctgcat ggcaaggttt gcctcgtaag taccgtgcat accgagcata
    14821 ccgaggaatt ggcggtcgcc ggaaggataa gcgcccaagc ccatcagcgt acccgtgcac
    14881 ggagcacccg tcattcggac aaatcgggtc agctcttcag aagcattacc caacaccacg
    14941 ccgccgccaa aatagacgac cggacgtttg gcagatgcca acatctgcac ggccttttta
    15001 atctgaccga tatgcccttg aacaaccggt tgatacgaac ggataaaaat gtcttcctga
    15061 ggatagctga atttcgccat cgcctgcgta acatctttcg ggacatcaac caccacgggc
    15121 cccggtcggc cgcttgcggc aatttggaac gcctttttaa tggtttccgc caactcattg
    15181 atgtccgtaa ccaggaaatt gtgtttgacg cacggacggg taatacccac cgtatcaact
    15241 tcttggaacg catccgtacc aatcagggaa ttgcctacct gcccgctgat gaccaccatc
    15301 ggaatcgaat ccgtataggc agtagcaata ccggtcagtg cattggtaac gcccgggccg
    15361 gatgtaacca atgccacgcc caccttaccg ctgacgcgcg catacgcatc tgccgcgtgt
    15421 actgccgcct gctcgtgacg ggtcagaatg tgcttgaatt tattgagttg gaaaagggca
    15481 tcgtagattt cgataaccgc accgccggga taaccgaaaa cgtactcgac accttcggct
    15541 ttgagactct gcactatgat ttgcgcgcct gatagctgca taacgacctc ttttatacgg
    15601 tttcaaacca atagggacaa accgctttgc cacagcacct gtaatgcaat tccaccaagc
    15661 agcgatttag ggtacgcgca ttgggggaac acggcaacag acggattatc caatcaattg
    15721 gaaaggaaca cagagtttgt gaaaaagagt agaaacgata acgcaaaccg acagttcaat
    15781 caagaaaaat ctttcatctt ttaattattt tttgaaagca gagaaattat tgattgattt
    15841 taaaagaata aaatcaggag tacctttttt gaaagatgga aattgttgac agtttgtgta
    15901 ggaggggcag atgtgaaaaa cccttcttcg atatcaagaa ttgtaaaatt tacagggttt
    15961 catcccaata aagactcggg atattgattg aacttgattt tatttttgat atatcaaaaa
    16021 tattcccaac catacttcct gaaaatggct cattgcaccg gactgtattg gacggcatta
    16081 acagaaccaa gagggctaac aacgacttaa tatattga
//
LOCUS       DAWKOD010000034        15370 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000034 DAWKOD010000000
VERSION     DAWKOD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.034"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJK73_000804"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJK73_000804"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7557317.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAKG
                     RKVDVIQGDGQFLDSHHLEVSLTAGDVYEQATPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WJK73_000805"
     CDS             complement(1906..2343)
                     /locus_tag="WJK73_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557318.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WJK73_000806"
     CDS             complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WJK73_000806"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ7557319.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAQAAAPAPAAAPAPTAVAAPAPSAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WJK73_000807"
     CDS             complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WJK73_000807"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ7557320.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKTLVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRVGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIEAERFNRLNPLETAKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRSFFEVDRYNVAVAALSA
                     LADQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7532..9244
                     /locus_tag="WJK73_000808"
     CDS             7532..9244
                     /locus_tag="WJK73_000808"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ7557321.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMASFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            9307..9963
                     /locus_tag="WJK73_000809"
     CDS             9307..9963
                     /locus_tag="WJK73_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ7557322.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WJK73_000810"
     CDS             complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WJK73_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ7557323.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10421..10969)
                     /locus_tag="WJK73_000811"
     CDS             complement(10421..10969)
                     /locus_tag="WJK73_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ7557324.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPFRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKILISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10971..11531)
                     /locus_tag="WJK73_000812"
     CDS             complement(10971..11531)
                     /locus_tag="WJK73_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ7557325.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11859..13664
                     /locus_tag="WJK73_000813"
     CDS             11859..13664
                     /locus_tag="WJK73_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7557326.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13791..15275
                     /locus_tag="WJK73_000814"
     CDS             13791..15275
                     /locus_tag="WJK73_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ7557327.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSETVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3714 a         3764 c         4224 g         3668 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgtttgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcagggat aaacggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtaggcg
     1141 ttgcttgttc gtacacgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acgtccactt tgcggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgatgccg tttgcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggggca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ctgcttcggc tttaggggcg gcagccgcac cttcggcttc gacgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agactgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaacagc ttcgccaaga atacggtaaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca atgggcacat caggcgcggg gcaaattctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 cggcgtgaaa cctgtgccgc cgatgccgcc caagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcgcg ggctttttta gacaattcgg tcaattcttg
     2761 gctaatttgt ttcaagcctt tttgatctac gtctttgatg acaggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaagt tgtcgccgtc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccaacggt gacagtttca cgccttcgcg ttcccattct ttgttcagct gtttgcggaa
     3001 ttcttccagc tcagtcatat ccgcttcttc gtgtacggta acgtggggaa tcacaaccca
     3061 gttgcgggac aggttttgac cggaaatttt cttaatgcgg gacaattctt taacttcgac
     3121 attgccgaat ttggagaagt ccactttagg ccacggcagc aagtccagac cgccgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc accgccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcga cagcagtcgg
     3421 agccggggcg gcagccggtg cgggtgcggc ggcttgagca ggggctgctg cggcagagcc
     3481 ggcggtttct acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     3541 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     3601 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc actttgattt caacggcgat
     3661 tacatccaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     3721 tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag gagcggcttc
     3781 ggcagcggcg gcaccggttt caacggtcag aataacgccg ccttcagaga ttttatcgcc
     3841 cactttaact ttaacttctt taacaacacc ggctgcatca gcaggtacat ccatagtggc
     3901 tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca atagtgtcgc ctactttgac
     3961 ttctacggcg atgatgtcga cgttttcatg accgccgata tcagggactt tgatttctac
     4021 gatactcatt tgagttcctt taatattttc agtttgatgc cgtctgaaaa tttgtttcac
     4081 acggcatcgg tacagtttgt tcaaaccatt caagcattcg gttttcagac ggcctgatgt
     4141 ctgaatgaag caaacaggcc gtctgaaaca tcaattagcg tttccagcta ggagctgaat
     4201 cggctttgat accatatttt tcaatggctt gttgaacggt ttctttgctg actttgcctt
     4261 gatcggccaa tgcgctcaat gcagcaacgg caacgttgta gcggtcaact tcgaagaagc
     4321 tacgcaggtt ggcacggctg tcggagcggc cgaagccgtc agtacccaag acgtggtagt
     4381 cgttagggat gtaggcacgg atgcggtcag catagctacg gatatagtcg gtagcggcaa
     4441 tcaccgggcc gtcatgacct tgcagttgag aagtaacaaa cggtactttt gcagtttcca
     4501 aaggattcag gcggttgaaa cgttctgctt cgatagcatc gcgatgcaac aggttgaaag
     4561 atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc tttcagcagc tcggcaccgg
     4621 caatcacttc ttgcaaaatc gtaccggaac ccatcagttg gactttcttg tcgcctttgc
     4681 cgccggcttt cagcaggtac atacctttca agatgtcttg ttccgcacct tcgggcatat
     4741 ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct tcattattgg
     4801 catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg aaagtcgggt
     4861 cataagatac gcagttcgga atcaggtcgg cctggatgtg gctgtggccg tcttcgtgtt
     4921 gcaggccttc gccgttcagc gtcgtacggc cggcagtacc gcccagcagg aagccgcgcg
     4981 cgtgcatatc accggctgcc catgccaagt cgccgacacg ttggaaaccg aacatagaat
     5041 agtaaatgta gaacggaatc atcgcgaagt tgctgttggc gtagctggtt gcagccgcaa
     5101 tccagtcggc catcgcgccc ggttcgttaa taccttcttg caagatttga ccgtcaacgg
     5161 attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga cctttcggat
     5221 tccaaatacc gtattggcgg aacatacctt ccatgccgaa agtacggctt tcgtcgggaa
     5281 cgataggtac gacgcgtttg ccgatttttt tgtctttcag caaagtggac aggatgcgga
     5341 cgaatgccat cgtggtcgag aactcgcgtt caccgctgga tttgagttgt gcgtcgaatg
     5401 ctgacagctc gggcacttcc aatacttcct gcgtcggttt gcgttgcggc aggtagccac
     5461 ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca
     5521 ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc
     5581 ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg ttctgacctt
     5641 cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg gtaggtttgc
     5701 cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg tcgtggccgc
     5761 cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgtt ttcagttcgg
     5821 gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt
     5881 cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct ttcgccaaga
     5941 ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg ccggcaaagt
     6001 tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc aagcgttgca
     6061 ggttgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc agtgcgattg
     6121 caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat actttacggc
     6181 ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc tgataaatcg
     6241 ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg ggcaagaggt
     6301 gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag ttgttcagct
     6361 ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg gcgacgtgac
     6421 cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt ggctttccaa aagtggttga
     6481 aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca cccagttcca
     6541 aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgga
     6601 tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc gaaacggtat
     6661 tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg cagtatttga
     6721 ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg acggagctta
     6781 acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg gtggacatag
     6841 tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac ccctttattt
     6901 tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga aaaaaataat
     6961 ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa acttgaaaaa
     7021 aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg caaataacca
     7081 tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag tctttttgat
     7141 ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat atcgaatcat
     7201 tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt tgtggataac
     7261 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
     7321 tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt caatatgatg
     7381 tttctggtca tgtttttgcc gtctgttatg ggatttgtcc cgcgcttggg gcagggggtg
     7441 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
     7501 tttgaatttg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
     7561 tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga ttcgcgccgg
     7621 cctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc tgcgcgtgtt
     7681 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagctgct
     7741 gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg aattttacgg
     7801 taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg gaccgacctg
     7861 cgaggaagtc atcgccgaca tcgtgcgtaa agagatcaac agctacaaac aactgccgaa
     7921 aaacttttac cacatccaaa ccaaattccg tgacgaagtg cgcccgcgct tcggtgtgat
     7981 gcgtgcgcgc gagtttgtca tgaaagatgc ttattccttc catgccgact acgcctcgct
     8041 tcagacgacc tatgatgcca tgtatgacgc ttactgccgt atcttcaccc gtctgggctt
     8101 ggaattccgc cccgttgccg cagataccgg cagcatcggc ggtaccggct ctcacgagtt
     8161 tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac agcgacactt ccgattacgc
     8221 cgccaatgtc gagctggcac cgaccttgcc gcttaaaggc gaacgcaccg ctgctcaggc
     8281 agaactcgtc aaagtacaca cgccaaacgt caaaaccatt gagtctttgg ttgaattcct
     8341 gaatattccg gttgaacaaa ccctcaaatc catcgtggtt gaaggcgaaa atgaaggcga
     8401 aatcgtttta ttgctgttgc gcggcgacca tgagttcaac gacatcaaag ccgaaaaact
     8461 cgcaggtgtg aaatcgccat tgacgatggc aagccctgcc gcgattgtcg aacagttcgg
     8521 cgcaaacggc ggctcgctgg gtccggtcgg ctttgcaggt aaagtttatg ccgatttcgc
     8581 cactgaaaaa ggcgcggact gggtcatcgg tgcgaacgaa gacggctacc actataccgg
     8641 cttcaacttc gggcgcgatg ccgccgagcc tgaattcgtc gatttgcgta atgtggtcga
     8701 aggcgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
     8761 acacgtcttc cagttgcgcg acaaatacac ccaagccatg aacgtgagct tcctcgacaa
     8821 caacggcaaa tcgcaaatca tggaaatggg ctgctacggt atcggcatca cccgcgtcgt
     8881 tgccgccgcc atcgagcaga acaacgacga aaaaggcatc atctggacga aggcgatggc
     8941 gtcgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacaccg tacgcgaagc
     9001 cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggatgaccg
     9061 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg ttgggtattc cgcaccgcat
     9121 cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa tacgccgaac gccgcgacaa
     9181 cgaagcgcag gcaattgcaa tcggagaaat tgttgcacgt gtaacagctt cattaaatgc
     9241 gtaagcttgg tcgaatgccg tctgaaaata atttcagacg gcatttttac ttagggggaa
     9301 agtgaagtgg cttcagtatt tttagaagga gaggcagtcg aggtcggcaa tattttctgt
     9361 atcggcagga actatgccgc acatatcgaa gagttaaaaa acgaaatacc ttccgaacct
     9421 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
     9481 gagtttagta gggatgtgca gtttgagtgc gaactcgtgc tgctggtcgg taaggattca
     9541 gacggcacgg gcgagggtaa agatattttg ggatgtgtcg caggatatgg tgtagggctg
     9601 gaccttacgg cacgggatat ccaatgccgt ctgaaggaaa aagggctgcc ctggctgaag
     9661 gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg cagaatcggc
     9721 aatcccgaga atgtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggat
     9781 accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc agattacggt
     9841 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
     9901 ggggacaatt tggcattgga gctggacggc ttggtgcgcg cctctttcac ggtagggtgt
     9961 taaccgcgcc gcttaacctt cacgcccgtt gaaacattcc gccggaccgc cctcgaaaaa
    10021 accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgccgaa
    10081 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    10141 atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg acaggtgcgt
    10201 catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat aacgcggctg
    10261 ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc cgccggtaac
    10321 ggtggccagc ggatgggaca gccccaattc cgcatcgccc tgcgccacgc cgatgcgcgc
    10381 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    10441 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    10501 aggggatgtc gaacaggata tgttcgtcgg cgggaacggt cagccacgca ttgtcggcaa
    10561 gttcgcgttc gagctggcct tttccccagc ttgaatagcc gatactgatt aatattttgt
    10621 caaccgcgcc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtc agcgcgatat
    10681 tgtccgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg acataacctc
    10741 gctcgacttg caccggtccg cccatcatca cgctgtcgtg ctgcattcgg aaagggatgt
    10801 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    10861 tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    10921 cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata tcagcgtttg
    10981 tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt gccggaagta
    11041 tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac cgcgcttaag
    11101 gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc gaaggttttg
    11161 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct cgcaccgtgt
    11221 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    11281 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    11341 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    11401 ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact tgcccgtccg
    11461 attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga cagcagtagc
    11521 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa ggcggcaggg
    11581 cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt atgcggctta
    11641 atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat atgacgcaca
    11701 atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa gattgggcaa
    11761 acggcatttt cagatggcat aactgacagt ataatccaaa cgtccggctg ctttcgtgca
    11821 gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa ccccttctgc
    11881 ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg gcacacaacc
    11941 gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa gcggcggaag
    12001 gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc ggcaaagagg
    12061 cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa gcagagaagg
    12121 aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa tcgaaggcgg
    12181 aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac gcaaaaacac
    12241 agcctgaaaa caagcaaggc aaaaaagagg caaaaggaca gggcaatccg cgcaagggcg
    12301 gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac aagaacggca
    12361 aagcagtgaa acaggataaa aaatacaggg aagagaaaaa tgccaaaacc gattccgacg
    12421 aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa gccttgctca
    12481 aacaaagcga aggaatgctg cttcatgtca gcaattccct caagcagctt caggaagagc
    12541 gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc aaccttgctt
    12601 ccgtcaaccg caaacagcgc gaggcttggg acaagttcca aaaactcaat accgagctga
    12661 accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc gtatcgggga
    12721 actataaaaa cagccagccg aatgcggttg ccctgttcct gaaaaacgcc gaaccgggtc
    12781 agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc ctccaatcgg gaagttgtca
    12841 aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc aacaatgagc
    12901 ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa cagggtgtaa
    12961 ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc aaagatgccc
    13021 gaaaactgct ggaacagaaa gggaacgagc agcagctgaa caagctcttg agcaatttgg
    13081 agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa ttggctgaag
    13141 ccagactggc ggcagccgaa aaagccagaa aagaagcggc gcagcagaag gctgaagcac
    13201 gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa catccaagcg ccttcggtga
    13261 tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg acgtttgaaa aaaccggttg
    13321 acggtgtgcc gaccggactt ttcgggcaga accggagcgg cggcgatgtt tggaaaggcg
    13381 tgttctattc cactgcaccg gcaacggttg aaagcattgc gccgggaacg gtaagctatg
    13441 cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga tcacggcgag aactacatca
    13501 gcatctatgc cggtttgagc gaaatttccg tcggcaaggg ttatatggtc gcggcaggaa
    13561 gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga ggggctttac ctgcaaatac
    13621 gttatcaagg tcaggtattg aacccttcga gctggatacg ttgatcggat tgccgaaacg
    13681 gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc cgtgaagttt gccgtttgcc
    13741 gtacctgtat tttccggttt ttggaaatca agagagaaag ttaaagaaga atgtcgaaac
    13801 ctgtttttaa gaaaatcgca ctttatactt tgggtgcaat cagcggcgtg gccgtcagtc
    13861 tggcggtgca gggttttgcc gccgagaagg acaggcggga taacgaagtc ctgccggtgc
    13921 aatccatccg cacaatggcg gaggtttacg gtcaaatcaa ggcaaactac tatcaggaca
    13981 aacccgatgc cgatttgttt gaaggtgcga tgaagggtat ggtggccggt ttggatccgc
    14041 attccgaata tatggataaa aaaggttatg ccgagataaa ggagtccacc agcggcgaat
    14101 ttggcggctt ggggatggaa atcgggcagg aagacggatt tgtcaaagtg gtttcgccga
    14161 ttgaggacac gcctgcggaa cgggcggggg tgaaaagcgg cgatttcatt gtgaaaatcg
    14221 ataatgtttc gacacgcggc atgacggtca gcgaaacggt aaagaaaatg cggggcaagc
    14281 cgggtacgaa gattactttg acgctgtcgc gcaaaaatgc cgacaagccg atagtcgtca
    14341 acctgacccg tgccattatt aaagtgaaaa gcgtccgcca tcacctgatc gaacccgatt
    14401 acggctatat ccgcgtgtcg cagttccaag agcggacggt cgaaagcgtc aataccgccg
    14461 caaaagagct ggtaaaggaa aataaaggaa aaccgctcaa ggggctggtg ttggatttgc
    14521 gcgacgaccc cggcgggctt ttgactggcg cggtcggcgt gtcggcggcg tttttgccgc
    14581 ctgatgcgct cgttgtcagt acgaaagggc gggaaggtac ggaaggcttt gcgttaaagg
    14641 cgcgtcccga agattatatt tttaaagccg gcacggatcc tttggcgggt attcctgccg
    14701 agttgaaaac cataccgatg acggttctga ttaattcggg ttcggcttcc gcgtcggaga
    14761 ttgtcgcagg tgcattgcag gatcataaac gcgcggttat cgtcggtacg cagagcttcg
    14821 gcaaaggttc ggttcagact ttgattcctt tgtccaacgg cagcgcggtc aagctgacaa
    14881 cggcactgta ttatacgccg aacgaccgtt ctattcaggc gcaggggatt gttcccgatg
    14941 tcgaagtaaa agataaggaa cgcatttttg aaagccgcga ggcggatttg gtcggacaca
    15001 tcggcaatcc tttgggcggc gaggatgtga acagtgaaac ccttgccgtg ccgcttgaaa
    15061 aagatgcgga taagcccgct gtaaaagaaa aaggtaaaaa gaaaaaggac gaggatttgt
    15121 cttcaaggcg gattcccaac cctgccaaag acgaccagtt gcggaaagct ttggatttag
    15181 tcaagtcgcc cgagcagtgg cagaagtctt tggggctggc ggcgaaaaag ccggtttcaa
    15241 ataaagataa gaaagataaa aaagataaga agtaggtttt tacgatgccg tctgaaagga
    15301 tttcagacgg catattatgt ttgaaaggac aggttatgag cgatttttta ccggaatttg
    15361 aaccgtgcgg
//
LOCUS       DAWKOD010000035        14903 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000035 DAWKOD010000000
VERSION     DAWKOD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.035"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJK73_000815"
     CDS             complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJK73_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ7557328.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEEHKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3821..4543
                     /gene="lpxH"
                     /locus_tag="WJK73_000816"
     CDS             3821..4543
                     /gene="lpxH"
                     /locus_tag="WJK73_000816"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ7557329.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSIAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            4812..6398
                     /locus_tag="WJK73_000817"
     CDS             4812..6398
                     /locus_tag="WJK73_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7557330.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6567..6770)
                     /locus_tag="WJK73_000818"
     CDS             complement(6567..6770)
                     /locus_tag="WJK73_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ7557331.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6886..7383
                     /locus_tag="WJK73_000819"
     CDS             6886..7383
                     /locus_tag="WJK73_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ7557332.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7465..8658
                     /locus_tag="WJK73_000820"
     CDS             7465..8658
                     /locus_tag="WJK73_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7557333.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(9062..9997)
                     /gene="hemC"
                     /locus_tag="WJK73_000821"
     CDS             complement(9062..9997)
                     /gene="hemC"
                     /locus_tag="WJK73_000821"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ7557334.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10116..10631
                     /gene="ybeY"
                     /locus_tag="WJK73_000822"
     CDS             10116..10631
                     /gene="ybeY"
                     /locus_tag="WJK73_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ7557335.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            10633..11457
                     /locus_tag="WJK73_000823"
     CDS             10633..11457
                     /locus_tag="WJK73_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ7557336.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11586..12965)
                     /gene="nhaC"
                     /locus_tag="WJK73_000824"
     CDS             complement(11586..12965)
                     /gene="nhaC"
                     /locus_tag="WJK73_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ7557337.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13256..14479)
                     /locus_tag="WJK73_000825"
     CDS             complement(13256..14479)
                     /locus_tag="WJK73_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ7557338.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3527 a         4209 c         3828 g         3339 t
ORIGIN      
        1 tttaaattta attcactata ccgtctgaaa acctgtatcg caacacgtca ttaagaacgg
       61 caagattcaa ccgttctgca acccgttcaa acggcttccg ccatttccaa cgcttgtttg
      121 atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tcgttacgcc gacgagctgc
      181 tctgccattt ccatcgtcag gcggttgtgg gagatgtaga ggaactgggt ttgcgccgac
      241 atttctttga ccaggttgca gaagcgcgag gtgttggcgt cgtccagcgg ggcatcgact
      301 tcgtccaaaa ggcagaacgg agcggggttg aggctgaaca gagcgaacac gaggctcatg
      361 gcggtgaggg ctttttcgcc gccggagagg aggtggatgg tgctgttttt cttgccgggc
      421 ggacgcgcca taatggacac accggcggtc agtaggtcgt cgcctatcat tttgagagtg
      481 gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt gacggcgtcg
      541 aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc ttcttccaaa
      601 atggtgatgg cggcctgcac gtcttcgctt tggctgcggt agtagccgtc gcgttcgcgc
      661 gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc gatttgttgc
      721 gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa cgcttcgagc
      781 gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc gttgatcagg
      841 gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg cagcttggtt
      901 tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg cgcgagtttc
      961 tcctgtacga caatatattc ttcgtccaaa ctctgtacgg cttcgctcaa ctcttcgagc
     1021 ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg caagggcaag ctcttgctgg
     1081 cgttcctgcc agtctagggt ttgctgttcg agtcgggcga tttgctgccg gtagttttgt
     1141 ttttgctggt tgagcttgtg gacggcgact tcggccagcc cgtattggcg gttggcttcc
     1201 aacagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct gtgtgcggtg
     1261 gtctgctgtt ggtgttcgag ttcggcggcg gcttcctgca aagtagcgat gtcgtctgaa
     1321 agcccgtcgg acgtgtgttg caacacggtc tgctcttccg ccaactgcgc cagttcgcgc
     1381 tcgatgtgtt cgcggcggat ttgcccttgg ttggtgcgcg ccaagagttc ggcggcgcgt
     1441 tgctgcgctt ggctgtattg gcgcgtgtgc tgctgttgct gctgcatcag gtttttatgt
     1501 tgcacttcgg acgagcgcac ggcggcttct gcctgtttga acgcggcttc gtcagcggag
     1561 agttcggggg ctaggttgtc cagttcggat gcgatgccgt cgaggcgcgc tttttgggca
     1621 atcaggcttt cctgcgcggg cttggcatag agcaggacgc tgaccttatc gacctgatga
     1681 ccttcgggcg tgagccagat ttgatgtgcg cccaaatcgc tttgatgcgc gagggcatag
     1741 ctcaaatcgg gcgcgcacaa tacgccgtcg agccagtggt gcaatgccgt ctgaaacggc
     1801 ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt gatgccgcct
     1861 gagaggtcgt ctgaaagcca tgccgcctgc ccctgcggca aaggctcggg cggcacgaaa
     1921 ccttgcggca cggcgcgggc gtgcaggcgt tcggcaagaa tgacggacaa ggcgtgctgc
     1981 cactcggcgg gcgcggtgat gtgttgccac agttgcggcg cggcggcgtg gtcggttgcc
     2041 tgccagaaat cggcggcttc ctgctgttgc gacaggattt gcgacaacgc ctgctgctgc
     2101 gcctgcaagg taatgtgttg ctgcttcagg ctttggaagc ggctcgatgc cgtctgaaac
     2161 gcctcgcggg cggcgtgtag agcttcttcg gcggcgatga tttgttcttc gtaatgctct
     2221 tgccggtttt gcaacaaggc ggcggcttcc tgcgcggcgg cggtttcggc ttcgtcgggc
     2281 aggttcaggg cttggttttc ctgtttcaga cggcctttgt gctcttcgtg cccggcaatc
     2341 gtttgtccgg tatgggcaag ctgctgctgt ttcaacgcca gttcgcggcg gatgcggttt
     2401 gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc ttccaattcg
     2461 ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc ggtttgcttt
     2521 tcttcgacgt ataactcgtt ttcttccagc tgcacgcgga tttgctgctg ctcttgatga
     2581 atacgctgca tctgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg gtgcaggttt
     2641 tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac accgcgtttg
     2701 ttgcccagtt cgtgtaccgc ctgctgctgc gactgttcgg cggtctgcaa ggcgtgtact
     2761 tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg caaagactga
     2821 tgctgcgcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc gtaatcgagc
     2881 aaatcctgtt gctggttcag ctgcgcggtc agggatttgt agcgttcggc ggtttccgcc
     2941 tgtttctcca gcttttccac ctgacgcgct aactcgttct gcaaatcgcc caaacgctgc
     3001 aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tacggcgttc cttatatttg
     3061 gacacgcccg ccgcttcctc gatataggcg cgcaattcct ccggccgcgc ttcgatgatg
     3121 cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc cgtacccaaa
     3181 aacaaatcgg taatgtcgcg gcggcgcacg gtctgattgt tgatgaaata agtcgattcg
     3241 ccctgacgcg tcagctgccg cttgatgctc acctcggcat actgccccca cgccccctgc
     3301 aaactgtggt cgctgttgtc aaataccagc tccaccgaag ccctcggcgc aggacggcgc
     3361 gtcgccgcac cgttaaaaat cacgtcctgc atactctcgc cgcgaagctg cttcgccgaa
     3421 gcctcaccca acacccagcg caccgcgtca atcacattcg acttgccgca accgttcggc
     3481 ccgataaccg cgacaagctg ccccggaaca tgaatcgtgg tcgggtcggt aaaagatttg
     3541 aagccggaga gtttgatgtg ggtcaggcgc ataatggtcg gaaaaaataa aaaagaagcg
     3601 tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc ttaccgcatc
     3661 ccgcacccgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc gaaccccggt
     3721 tccgccaatg cccgatgctt ggcgcggcat ttgccgtatt gccccgccgc cgggcgcgtt
     3781 ataatccgcc acgacatccg cccgatttgg aaacctgcct atgaaacccg cctatttcat
     3841 ttccgacctg catttgagcg aaaagcagcc cgaactgacc gcgctgttgc tgcgtttttt
     3901 acgttcttcc gccgccaggc aggcgcgggc ggtttacatt ttgggcgatt tgtttgattt
     3961 ttgggtgggc gatgacgaag tttccgagtt gaatacttcg attgcgcgtg aaatcaggaa
     4021 attgtccgac aaaggcgttg ccgtgttctt cgtcaggggc aaccgcgact tcctgatcgg
     4081 tcaggatttt tgccggcagg cgggcatgac gctgctgccg gattactcgg ttttggactt
     4141 gttcggctgc aaaaccctca tctgccacgg cgacacatta tgcaccgacg acaaggcata
     4201 ccaacgtttc cgcaaaatcg tgcatcggaa gcggctgcaa aaactgttcc tgatgctgcc
     4261 cctaaagtgg cgcacgcgcc ttgccgccaa aatcaggcgt gtcagcaaaa tggaaaaaca
     4321 ggtcaagccc gccgatatta tggatgtcaa tgccgccttt accgcgcggc aggtacgcgc
     4381 cttcggtgcg gaaaggctga tacacggaca cacccaccgc gagcatatcc atcacgaaaa
     4441 cggctttacc cgcatcgttt tgggcgactg gcataacgac tatgcttcaa tcctccgcgt
     4501 ggacggggac ggcgcggtat tcgtgccgct ggaaaaatac tgaaaatgcc gtctgaagcc
     4561 tttcgggcgg ctttttttac acgccccttt cccacactcc ctccgccgca tcatcaccat
     4621 ccttaacccg cattaatagc cattatcgat aaaatgcttg accgattaca taagattacg
     4681 taaagtgtgc aaacgcataa caattggtct taccaattta tcttttaacc aaaatatcgg
     4741 gtgtacggga tttgaacgtc tttccaaacc tcgacgcgcc ttatgatgca aatgcctaaa
     4801 aaggattcgt tatggcactt ttcctcagca tattccccat cgtcctgctg atttggctga
     4861 tggtgaaaaa aaacagtatg ccctcctacg tcgcgctgcc gattaccgca gtgctgattt
     4921 acgccatcaa acttttctac ttcgacgatg cgggcatgct gctcaacgcc accgccgctt
     4981 ccggcctcgt caaaacgctc acgccgatta ccgtgatttt cggcgcgatt atgttcaacc
     5041 gtatgatgga aaccacgggc tgcatcgatg tcatccgcaa atggctggcg accatcagcc
     5101 cgaaccccgt agcgcaactg atgattatcg gctgggcttt tgcctttatg attgaaggcg
     5161 catccggctt cggtacgcct gccgcgattg ccgcgccgat tctgatgagc ttgggcttca
     5221 acccgttgaa agtggcgatt ttcactttgg tgatgaactc cgtccccgtc tctttcggcg
     5281 cggtcggtac gccgacctgg ttcggtttcg caccgctgaa cctgagtgcc gaagacatcc
     5341 tcgccatcgg caggcagacc ggcgtaatgc acttcttcgc aggtttcgtc atccccgtca
     5401 tcggcttggg cttcatcgta ccttggtctg aaatccgcaa aaacttgggc ttcgtcgcca
     5461 ttgccgtctt ctcctgcacc attccttatg tcgcattggc gatggtcaac gaagaattcc
     5521 cgtcgctcgt cgccggcgcg atcggcctga tggtgtccgt attcgccgcc aaccaaggct
     5581 ggggcttgag caaagaccac gccaaagacc cgaatgccga aaaagtgccg ttcgcccaag
     5641 tcgccaaagc actcgcccct ttgggtatgc tgatcggcat gctggtggtt acgcgcatca
     5701 aacagctcgg catcaaaggc attttgacca gcaaagaaga atggttcagc ttccaactgc
     5761 cgtttgattt gtccaaaatc accgtcagcg actccctgac gattactttc ggcaatattt
     5821 tcggacaaga tgtcagcgcg tcttaccaaa cgctgtacgt cccggcttgg attccgtttg
     5881 tgctgaccgt ttggatttgc atcctgctgt ataaaaccaa attcaaagat gcctggacga
     5941 tttatgccgt aactttcaat caaaccaaaa aaccgctgct tgccctgatg ggcgcgctga
     6001 ttatggttca gctgatgctg gtcggcggcg acaattcgat ggtgaaaatc atcggtaagg
     6061 aatttgccgc aatggcgggc gaacactggg tttatttctc gccgtatctg ggcgcgatcg
     6121 gtgcgttctt ctccggctcc aacaccgtgt ccaacctgac cttcggaccg attcagcagc
     6181 aaatcgcctt ggataccggc ctgtccgtta ccctgattct ggcgttgcag tccgtcggcg
     6241 gcgcgatggg caatatggtg tgcctcaaca acatcatcgc cgtatgtacc gtattggacg
     6301 tgaaaaattc cgaaggtgcg attatcaaga aaaccgttat cccgatggcg atttacggcg
     6361 tgattgccgt cgtcgcggca atgattttct tcctctaggc gaaacgccac catccatagc
     6421 ggcagcacgc ggggaacgga aaccgccgtt ccccgtccgg gcggcgcgtc gggcagaatc
     6481 ccggtgccgg aacaagcccg ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc
     6541 agacggcatt tttccgccgc ttgcgttcag gcgtaatgat gatgcggcat cttgccgtca
     6601 aggcggtagc gcgtgccgca atacgggcag gcaacgctgc ccgattctcc ttcgcacaaa
     6661 ggcaaaaaca ctctcggatg cccgttccac tgctcgttgt cgggtcccga gcaatacagc
     6721 ggcagatttt ccggcaacac ggaaatttcc tgcagattca gattgtccat ttgattttcc
     6781 tttgcgtggt tggcgtgcgc ccattttacg ccatcggcag gctaaaggat attttcggcg
     6841 caaagccgca atccgctata atccccactt ttcagacggc ataccatgac tgcgcttacc
     6901 cttccggaag acatccgcca acaagagcca tccgccctgc tctataccct cgtttccgcc
     6961 tacctcgaac acaccgccca aaccggcgac gaatccctct cctgcctgtc cgacgaccag
     7021 cacacgctga ccgcattctg ctacctcgac agccaagtcg aagaaggcgg cttcgtgcaa
     7081 ctcatcgcct ccggttacgg cgaatatatt ttccgcaacc cgcttgccga cagcctgcgc
     7141 cgctggaaaa tcaaagccgt gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa
     7201 cacggcaaaa ccatcgaaac gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa
     7261 cagttccccg actttgaaga atgggacggc gcatactacg aagccgccga acaagacctg
     7321 cccctgcttg cagaacacat acagtcaaac tgggaaacct tcgcccatat cgggcaggcg
     7381 tgattgcgtc tgtttccagc ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaatgacct
     7441 tccgtcccca tcaaggagca agctatgttc ttcaagcaca tcgaagccgc ccccgccgat
     7501 ccgattctcg gtttgggcga agcgttcaaa gccgaaaccc gccccgaaaa agtcaacctc
     7561 ggcatcggcg tttataaaga cgcatccggc gcgacacccc tcgtcaaagc cgtcaaagaa
     7621 gccgaaaaac gcctgttgga aagcgaaaca accaaaaact acctcaccat cgacggtgtt
     7681 gccgactaca acgcgcaaac ccaaatcctg ctgttcggca aagaccacga aatcatcgcc
     7741 agccgtcgcg ccaaaacagc gcaaagcctc ggcggtacgg gcgcattgcg tattgcggcc
     7801 gagtttgcca aacgccagtt gaacgcgcaa accatctgga tttccaatcc gacttggccc
     7861 aaccacaacg ccatcgccaa agcggtcggt atccaagaca aaccttatcg ttactatgat
     7921 gccgccaaac acggtttgga ttgggacggc atgattgagg acttgagcca agcgcaaaaa
     7981 ggcgacatcg tcctgctgca cggctgctgc cacaatccta ccggcatcga ccctacgccc
     8041 gaacaatggg aaactttggc aaaactttct gccgaaaaag gctggttgcc gctgtttgac
     8101 tttgcctacc aaggcttcgg caatggtttg gaagaagatg cctacggctt gcgcgtgttc
     8161 ttgaaacaca atacagaatt gctgattgcc agctcttatt ccaaaaactt cggtatgtac
     8221 aacgagcgcg tcggcgcgtt cactttggtg gccgaagatg aagaaacagc agcccgcgcc
     8281 cacagccaag tcaaaaccat catccgtacc ttgtattcca acccggcttc acacggtgcg
     8341 aacaccattg cgctggtgtt gaaaaatgat gatttgaaag cacaatggat tgccgaactc
     8401 gatgaaatgc gcggccgcat caaagccatg cgccaaaaat ttgtcgggtt gctcaaagcc
     8461 aaaggtgcaa gccaaaactt tgatttcatt atcaaacaaa acggtatgtt ctctttcagc
     8521 ggcttgactc ccgaacaagt cgaccgcctg aaaaacgagt ttgccattta tgccgtccgc
     8581 tccggccgca tcaacgtcgc cggcattacc gacaacaaca tcgattatct gtgcgaaagc
     8641 atcgtgaaag tactgtaaaa agcacagcca tatgaaaatg ccgtccgaac cttaaccagt
     8701 tcagacggca tctgtttttt gttaaaaaac aataaaccat aacaatttca aacaagctaa
     8761 aaacatctat aaaatcaacc aaatatgatg aactcaaata aaaaatacga atatcatcat
     8821 tctcaaattc aaaatttgaa cgccacagta ttatccgaag ccacaaaaac ctttccgccg
     8881 tcattcccgc gcaggcggga atccgggaat ccaatgctgc aagaattgat tagaaacaac
     8941 tgaaaccgag caaactggat tcccgcctgc gcgggaatga cgaaataagg cgagtgaaac
     9001 tgatgaaaca aattgattaa aaacaataac ttatattatt ttatttaaat tttcgataaa
     9061 atcaattttc cgtattcaat actgctccaa tcaattcccg cgcaccgtcg tccgccaatt
     9121 tcttagcgac agcgcgtccg agcgcgtcgg catattcggc aggggcttgc gcgtccgccc
     9181 gcaacacaac cgaaccgtcg gggtgtccga ccaagccgcg caaggtcagc aagccgtttt
     9241 cttccgtgca atatgcggcc aaaggcactt ggcagcttcc gcccaaagcg cgtgcgaggg
     9301 cgcgttcggc ggtaacgcag gcattggtaa caccgtggtt caagggtttc aaaacttcat
     9361 acaaatcttc gcggtgcgcg gcaatttcga tacccaatgc gccttgtccg gcggcaggca
     9421 ggctgtcgga ttccgacaaa atcatgcgga tgcgtccgtc caatttcaga cgctgcaaac
     9481 cggcggcagc caagataatt gcgtcgtatt cgccgttatc gagtttggac aaacgggttt
     9541 gcacattgcc gcgcaaaggt ttgataagca aatgcggata gcgcgcacgc aactgggctt
     9601 cgcggcgcag gctggatgtg ccgacaaccg cgccttcggg catttcttcc aaacgcgtgt
     9661 attggttgga cacaaacgcg tcaaacggat tggcgcgttc gccgatggcg gcaagcgcga
     9721 aaccttcagg caaatccatc ggcacgtcct taatcgaatg caccgccaaa tcggcgcgcc
     9781 cgtcatataa agcctgttcc aactctttga caaacaagcc tttaccgccg acttttgaca
     9841 aagttttgtc caaaatctga tcgccgcgcg tggtcatgcc caaaatctcg acttcgcaat
     9901 cgggatacag agccttcaga cggccttgga tatgctttgc ctgccacata gcaagcaggc
     9961 tttcgcggct ggcgatgacg agttttttcg ggttcatgtt caatgcgttc aaagaatgga
    10021 aacggcgtga agtctatcat attttgaacg tttcttcaga cgacaccgtc aggtctatgt
    10081 tatatttaca cccacaaaac acgaaacccg ccacaatgaa acgcaccaaa aaataccctt
    10141 tcttaacgtt gcagcggcaa cgtttccatt tgaactttga aaacgcctct tccgccgccg
    10201 gcatcccagc cgaacgcgat ttctaccgct gggcgtggtc tgccttgaaa aatgaatacc
    10261 gccgcgccga catcagcctg attcttctgg acgaagaaga agcccgagcc tacaaccgcg
    10321 actaccgcgg caaagattac gccaccaatg tattgagttt cgcgctcaac gaaggcgaaa
    10381 tcctgccctg ccaagtttcg gaaaaactgt acggcgattt gattatctgc ccgcaagtgg
    10441 ttttgaaaga agccgccgaa caaggcaaaa cacccgagca gcattttgcc cacctgacca
    10501 tacacggtac tttacacctg atgggctacg accacatcga agacgacgaa gccgaaataa
    10561 tggaagccga agaaatccgc ctgatgcggg cggcaggctt ccccaacccc taccaagagg
    10621 acggacatta aaatggacgg cgcacaaccg aaaacgaatt tttttgaacg cctgattgcc
    10681 cgactcgccc gcgaacccga ttccgccgaa gacgtattaa acctgcttcg gcaggcgcac
    10741 gagcaggaag tttttgatgc ggatacgctt ttaagattgg aaaaagtcct cgatttttcc
    10801 gatttggaag tgcgcgacgc gatgattacg cgcagccgta tgaacgtttt aaaagaaaac
    10861 gacagcatcg agcgcatcac cgcctacgtt atcgataccg cccattcgcg cttccccgtc
    10921 atcggcgaag acaaagacga agttttgggc attttgcacg ccaaagacct gctcaaatat
    10981 atgtttaacc ccgagcagtt ccacctcaaa tccattctcc gccccgccgt cttcgtcccc
    11041 gaaggcaaat cgctgaccgc ccttttaaaa gagttccgcg aacagcgcaa ccatatggcg
    11101 attgtcatcg acgaatacgg cggcacatcc ggcttggtca cctttgaaga catcatcgag
    11161 caaatcgtcg gcgaaatcga agacgagttt gacgaagacg atagcgccga caatatccat
    11221 gccgtttctt ccgaacgctg gcgcatccat gcagctaccg aaatcgaaga catcaacacc
    11281 ttcttcggca cggaatacag cagcgaagaa gccgacacca ttggcggcct ggtcattcaa
    11341 gagttgggac atctgcccgt gcgcggcgaa aaagtcctta tcggcggttt gcagttcacc
    11401 gtcgcacgcg ccgacaaccg ccgcctgcat acgctgatgg cgacccgcgt gaagtaagca
    11461 ccgccgtttc tgcacagttt aggatgacgg tacgggcgtt ttctgtttca atccgcccca
    11521 tccgccaaac ataaaaatgc cgtctgaaac cgttgcaagt ttcagacggc atttcgctta
    11581 tccgcttatt ttttgctcaa agtcagcccc gtccaaccga ataacagggt caaagccaaa
    11641 ctcaaatagc agaaaaaggc atacggcaga tattcccaaa ccggcacgcc cagcgcgtgg
    11701 ctgatgaaca cgccgcatac gctccacggt acgagcgggt taatcaccgt ccccgcatct
    11761 tccagcgtcc gcgacagatt gcgcgaatgc agaccgagct tatcgtaaac gggtttgaac
    11821 gtttcacccg acaacaaaat actcaaatat tgctcgccga tcaggaaatt aaccccgacc
    11881 gaagtcatgg caacgctgaa cgtcgcgcgt ccggcattcg tcaagaaggt acggatggcc
    11941 tccaacaggg aaggaatcac accgagcgca aacaacagtc cgcccaaact catcccgaga
    12001 atcacgatgg tttgcgtgaa aaacatactt tccaagccgc cgcgcgaaat gagtttgaca
    12061 acatctttaa acgcttcgcc ttcgagtttg taaccgccgt aaaaccacgc gccgagctga
    12121 cgcagatcgg gcgtgctgtg cagatacgtt acggcaacgg caaccatgac ggtaaagagc
    12181 atggcgacca cggcgttgac gcgcatcaat gccaaaatga ccaacagcgc aaacggaatc
    12241 agcgaatagc cgtgcaccaa tcccgtggct tcaagctggc tgcggaagga ttcgacgctg
    12301 ttcaaatcct gcgcggcgac actgggaaga agccacagca tcagtgccgc actgatgagc
    12361 cacgcgggaa tggtggtgta catcatattt ttgatgtgct caaacaggtc gatgccgacg
    12421 atggacgcgg aaatgcccgt ggtgtcggaa agcggggaca ttttgtcgcc gaaaaacgca
    12481 ccggaaacaa tcgcgcccgc cgtcatcgcc atatcggcat gaaacgccgc cgccatcccc
    12541 ataaaggcaa cgccgacggt ggcgcaggtg gtcaggctgc tgccgatgga tacgccgatg
    12601 acggaacaca gcgcgaaagc ggaaaaataa aaataagtcg gggaaatcag cccgaaaccg
    12661 taatacatca gcgtcggaat cgcaccgctc atcatcagcg cgctgaccat cagcccgatg
    12721 aagaaaaaca ggtaaatcgc gcccataccc tgattcaacg cgcctatcat gccctgctgc
    12781 atatcgttgt atttcaaacc gcgcgccaag ccgtacaaaa tcagcacgac gatggcggca
    12841 ataatggaca tatgcggcag ccactccaat gaaatgatgg tatagcccat cgcggcaatc
    12901 agagcgacga cgacggcaag tgcctcaccg cgcggcatat cgagtaagga tttgaaagcg
    12961 aacatgattc tttgcccgat ttgatataaa gttaacggat tctaacacac tataccgaat
    13021 ccgcccgacg ttttcagacg gcattttagc gttttacaaa agccggacag aaacaaaacg
    13081 gcaattttct caatttaaat taattttttt gaaatataat tttttatttt tttgtttgtt
    13141 gacatataag acttttatgg attgaagtat taaaacctcg gcacacacgc cgcaaacacc
    13201 ggtacgcaaa aatgccgtct gaaagatgtt cagacgacat ttctcaagaa aaagattatt
    13261 tgtccgccct atatccgtac accgcaaaaa acacgatata cagataacag atgacggaaa
    13321 cgacaaacga agacatcagg gtgtaagtat ctgccaccca gccctgcacg acaggaacga
    13381 ccgcaccgcc gacaatcgcg gtacacagta caccggaagc cgcgttggta aattttccca
    13441 atcctttggt tgccaaagag aaaatcgtcg ggaacataat cgagttgaaa aaaccgatgg
    13501 caagcagcga ccacatcgcc acatccgcat tgccgctacc cgtcgccatc gcgacggcaa
    13561 gcagtacgac cgcagccgat gcgttaaacg ccaaataacg gttgggcgcg aatttcgcca
    13621 tcaccgccga accgaggaaa cgtccgacca tcgcgccgcc ccaatagaac gacaggtaat
    13681 gcgcggcaga agcatgatcc agccctttca gataacccaa tacgttgacc atcaacgaac
    13741 cgatagacac ctccgcgccg acatagcaga aaatgcctgc cgtgccgaac acgagatgtt
    13801 tgtattgcca tacgctggtt ttgccgtcgt ggttgtgcgc gctttcctcg gcggcaattt
    13861 tgcgcgcgtc gggcagccgg atcattttca cgaaaacggc aaggataatc agcagccccg
    13921 ccagtcccaa atagggaatc tgtacggaag aaatctgttc cgccttgctg acggtttggg
    13981 ttgcgtccgc cagaatcagg aacgcgccga tttgcggcgc aatggtcgta cccaaagcgt
    14041 taaacgcctg aaccagcgtc agtgttgccg attccttgcc aggtttcgcc agcagggtaa
    14101 cataaggatt accggcgacc tgaagcagcg ttacgccgga agccaaaata aacaacgcgc
    14161 ccaaaaatac cgcgtaagaa tggctacccg cagcaggata aaacagcagg catccgaccg
    14221 ccgtcagcag aaagccgccg ataacgccgt ttttgtagcc gactttgccg acaaaagccc
    14281 ccatcgggat ggacatcacc gcataggcgg taaagaaaca gaattggatc agcatcgcct
    14341 gaacgtaaga caggtcgaaa atttctttca aatgagggat aaggatgtcg ttcatgcagg
    14401 taataaaacc catcatgaag aacagcgtgg tcaagacgac caatggggac gtatgattgt
    14461 tttgtgattg cgcagacatg atttgccttt cgtggtgtcg gaatattacc cgtttcaaga
    14521 aaatagaaat tgacatttct taacggcaat gcaaatcata ctgatatttg aacattttgc
    14581 aaactttatc ggatgcaggc aatgcgccga tctgaaattc aagcagcaga aattaatatt
    14641 ttacagttgc ttgcatagat tttaatagat tttaaaaaat tccggcggac ttgcaaaaac
    14701 cgttcccaat aaaatgaaaa tccgccgccg tatccggaaa attcggaaat gccgcatcgt
    14761 ggcatcaaaa ccggagcgga aagaatgtcg tatgcccgac aaaaaagggg catacggctg
    14821 tgccgtattt tttgcaccgt cctccgccct tcggcggggg cgtgaaaata tagtggatta
    14881 acaaaaatca ggacaaggcg acg
//
LOCUS       DAWKOD010000036        14856 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000036 DAWKOD010000000
VERSION     DAWKOD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.036"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..815)
                     /locus_tag="WJK73_000826"
     CDS             complement(120..815)
                     /locus_tag="WJK73_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7557339.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(986..1645)
                     /locus_tag="WJK73_000827"
     CDS             complement(986..1645)
                     /locus_tag="WJK73_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ7557340.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1843..3955)
                     /locus_tag="WJK73_000828"
                     /pseudo
     CDS             complement(1843..3955)
                     /locus_tag="WJK73_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4141..4746)
                     /locus_tag="WJK73_000829"
     CDS             complement(4141..4746)
                     /locus_tag="WJK73_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7557341.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4795..5193)
                     /locus_tag="WJK73_000830"
     CDS             complement(4795..5193)
                     /locus_tag="WJK73_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7557342.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5301..6212
                     /locus_tag="WJK73_000831"
     CDS             5301..6212
                     /locus_tag="WJK73_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7557343.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6202..7503
                     /locus_tag="WJK73_000832"
     CDS             6202..7503
                     /locus_tag="WJK73_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ7557344.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELNKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7566..7862
                     /locus_tag="WJK73_000833"
     CDS             7566..7862
                     /locus_tag="WJK73_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557345.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7896..10046
                     /gene="dinG"
                     /locus_tag="WJK73_000834"
     CDS             7896..10046
                     /gene="dinG"
                     /locus_tag="WJK73_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ7557346.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10082..10696
                     /locus_tag="WJK73_000835"
     CDS             10082..10696
                     /locus_tag="WJK73_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ7557347.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10791..12659)
                     /locus_tag="WJK73_000836"
     CDS             complement(10791..12659)
                     /locus_tag="WJK73_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7557348.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12740..14110)
                     /gene="purB"
                     /locus_tag="WJK73_000837"
     CDS             complement(12740..14110)
                     /gene="purB"
                     /locus_tag="WJK73_000837"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ7557349.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14182..14814)
                     /locus_tag="WJK73_000838"
     CDS             complement(14182..14814)
                     /locus_tag="WJK73_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ7557350.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3543 a         4111 c         3828 g         3374 t
ORIGIN      
        1 ctatatattc tgatttaaaa ccataaggct ttaagcaatc atctcttcta taaagcctaa
       61 atacaaaagg ccgtctgaaa tcctattttc agatggcctt tactcattca atcctcaact
      121 tattgcggct ttttctgctc ttcgcgtact ttatccacca actggtcaat cgtggtcata
      181 ccggactgcc agtctttaaa ttcaacttgg tatttgccgc cgacaatcac agtcggtgtg
      241 ccgctgattt ggaatttatt ggtcaactct tccatttgag ccgcacgcgc ttggctttca
      301 ggagcctcaa atgcagccaa tacttttttg ccgtcaaacg ctgtttgctc ggacagccat
      361 tttttcaggg tatcggtatc ggccagattg attttttgat taaccatcgc atcgaaaata
      421 tggctgttgg ctttatctga ttcaccggcc atttccactg cggccgccaa acgtgccaaa
      481 ggtttcattt catcacccca cacgacatgc tcccggcgca tataggtatc gtctttaaac
      541 gttttgatgt gctcgctcaa gaccggctca agatgggcgc aatgcgggca gaagtagccg
      601 aaaaattcca atacttcgat tttaccggcc tgctgttgcg gaataggcgt agacaataca
      661 gtgtagttca caccttcgtt caactcagca ggggctgccg gagcagatga gctgctttgg
      721 gcgctgtctg ccggaacact ggtttcagcc tgtttgctac aagcggccaa tgccaacagg
      781 gtcaatgaag tcaaagctaa ggttttcagt ttcataggta tctcttgtgt gtcagattaa
      841 tgtgcggatt ttatggcatt ttattgaagg cgtgttcgat ttgagtgaaa aaatgtgttt
      901 agttttattt cagcctttat aaaggccgtc tgaaatatgg cttagataat tagccttatt
      961 ttcagacggc ctttgttcta gtaatttacg gtttcggaca ttttgtcttc aatccgtcga
     1021 tgtgttccat taagtcaggt gggacatgat tccacatagg atcaagaata aaatcgatac
     1081 cttcccgtct ggctaatttg gctgctggga caaaatcgga atcaccagaa aacaagatga
     1141 ttttatcggc ttgttttttt aaggtaatag atgaaatatc caagcctata cgcatatcta
     1201 cacctttttg tttaacatcg agcacgacat cgttttccgt aatttgagtt gacgggtttt
     1261 tcagaagatt tttcatgact tggggtttta atatccatgc tgtattgtcc gacagcttgc
     1321 ctaaacgtaa cgctaattta cgcttcttct tcaattcggt atgcagattg caacgaaata
     1381 tggcttccgg agtctttgaa aaatctatgg cacgatgaga tacgggattg tgcattttct
     1441 tttcaaacgg cacgcagtcg taaaagaaaa ttctatataa atcatccatt tgatctgtgc
     1501 cgttgatttt atctttcaga tgcagcatcg aatatcggaa agccaaatca gcaattcttt
     1561 ttgcatcgtg tggattttga gacaggccta atgaccgtac ccgcttaata aaaaatgcac
     1621 cgtcaatcaa aatggcggtt ttcatattgc ttcccctata tttgtcaaag atataaaaaa
     1681 gcccttggga tcggcatacc cgctatgatt taatgggtaa tgcgtactgc caagggcact
     1741 atttcttttt ggattatact gatgtagata aagcctgtca aaaagttcgt atgctgaatt
     1801 tacggattat caggattttc agacggcctt tgcctgattt catcagaaac ggtaatttac
     1861 ccgggcagta tagccgcgcg gattacccgg catagagtcc gaacgccaat atttttgatt
     1921 gaacagattg gctgcggcaa aggtaacgtt aacattttta tggttccagc caagcatggc
     1981 atcaactcgg gcaaagcctg gaagcgtagt cacttcttta tttcttgagt cgtaaccgta
     2041 gcgtttgcct gtaccggtta cgccgatttc gccgtagagg ttttcggtcg gggtataacg
     2101 gaaaaacagg ttgccggtaa cgttgctggt gttattcaaa tggatgccca ctcggtcggg
     2161 attttctttg tcttcaacga ctttcgcctg catcacgccc aacgaaccgc gcagatagag
     2221 tttttttggg gatgatttgc ccgatggcgg acaattccac gccgcgcgaa cggtgtttgc
     2281 cgctaaccgc ataaatataa gggttgtttt ttggatcggg gcggtagcgg atattgaagc
     2341 gttcgatttg gtaggcagac aacgtagtgc tgaggcggtc gtccagccaa ctgcttttca
     2401 cgccggtttc gtattggcgg gtgtactcgg ggtcggcgtt gaacacggcg gaagacaacg
     2461 tatcgatgct caaatagccg ccgcgtccgc cataaggcgc gaagcctttg ttatacgagg
     2521 cgtaaagtgt gtggacggga ttgatgttcc acactgcgcc gatgttgggg ctgaacgagt
     2581 gtccgctgta ttggcggctg ctgccggtga gtttgttttc ggaattaaag gtgtatttgt
     2641 cgtaacggcc gccgaggacg aatttcaaat cgggcgtggc ggagaagatg ttttgcacaa
     2701 agatgccgta ggagtcggct ttgtggcggt tttgggtcag aataggctgc aatctgcccg
     2761 aagccggcca gcttgcgcgg tcgtaggggt tgatggaggc ggaaaaggcg ctgctgaaac
     2821 ccaatgtcgg gttgcggtgt tcgcggctgt aatccatgcc tacggtcagg tggttttcaa
     2881 aacggccgat ggtgtagtcg ccgttgagcg ttaagttgga cgacagggtt ttgttgtcgg
     2941 tctgctgcca ggcgtagtta cgtttgatta agttgccatt ttcgctgcct gcatagaaat
     3001 gatcaaaatc ctgcgccgcc gtgcggtggg cgagctgcca ttgggcacgc catttgtcgt
     3061 tgaaggcgta ttcaaggtcg gaacgccaaa cttgcagctt gtctttgaca aaatcgttcc
     3121 ggtgggcgaa ccccatgcgg taaggcagtc cgaagcggtc gtacacggac ttggtcggac
     3181 tgcggtcggg cgtgcgctcc acattgtcgt aggtgtattg ccccgtccac ttcaagccgt
     3241 tgtcgagttt gacggtaatg ctgggcgaaa ccatgacatt tttgctgtct atgccgctgc
     3301 ggaacgaatt ggcgcgcccg acttcgccgg tgagacggat ggcgacgttt ttgttcagca
     3361 cttcgttgat gtccatattc aggctacggt ttgcccacga accataaacc gtaccgatat
     3421 tacggctttg tttgaagttg gcgtatttgc tgaccatgtt gatgacaccg ccgccgttgg
     3481 tacgcccata aagcacggag gacggacctt tcaggatttc cacgcgctcg atgttggcgg
     3541 tgctacggcg cacctgcccg ctttcgcgta cgccgtcgcg gtaaatatca gatgcgtcgg
     3601 cttgaaagcc gcgcaggaaa atgctttcgc cgcgcatatc gtaggcggcg tcgatgccgg
     3661 cattgccttc gaggatggaa ctcaaatcgt tcgtgccgta atttttgttt ttctggatat
     3721 tgagcgtatc gatggtttgc ggcgtttctt tgatgagctg tccgttgcgg gtaacggcgg
     3781 cttcgtcgta gttgatgtag cctttgagta cgctggtgtc ggactgtccg accacggaaa
     3841 cggtaggtag cgtggcggtg taatgttcac cattgtcctg cgtatcggcg gcagcaacag
     3901 ggaaggaagc aataatcagc gtgggtaata aagccaaatg aaatggtatt tgcattttta
     3961 tactcaattt aacaaaacaa ccgaattata ttgcctcacg gaggaaatga gaataatttc
     4021 ttttaactat attgaacatg atatttgtaa acaaaggtct cagaatgcgg aaaactcgcc
     4081 gcctgatact gaaaaatgcc gtctgaacag ggttcagacg gcattttttt gaccgcgaaa
     4141 ttatgcgccg aacactttca aacgttctgc aactggttca aaggtctttt cacctgccgc
     4201 cccttcaata ccgccgataa ccatttgtgc gcgcaacaac cagttttcgg ggatattcca
     4261 cgctttggca atcgccgcat cgggcaaggg attgtaatgt tgcaggtttg cacctacgcc
     4321 gaccgcggca agtgtcgtcc aaacggcata ctgcaccatc gcgttcgcct ggtccgccca
     4381 aacgggaaag ttggcggcat aagcagggaa ctgctcctgc aaacctttga cgacattttg
     4441 atcttcataa aacaaaatgg ttgccgcacc cgccttaaac aggttcaatt tttgcgcggt
     4501 cggttcaaaa ctgtcggcag gcacggcggc acgcagcgcg tcttcgacaa attgccacac
     4561 cttatcatgc tcttcgccaa acagcacgac cacgcgggca gattgggaat tgaacgaaga
     4621 aggtgtgtgc aaaacggcgt gttcgacgat ttggacaact tcatctttgc cgacgggcag
     4681 atttttattt aacgaataaa tggaacggcg gctttcggca gcctgttgca gagattgacg
     4741 ggtcatttct tttcctcaaa aacattcaac aaaatacact ttaggcagtc cgattcaggc
     4801 tttacgcttg aaaaacagcc tgtccagcga ccatgctccg ccgcccgccg ccgcgatata
     4861 gaggaatacg aagcagaaca gcactgcgga ctcgccgccg ttggcaatcg ggaacaaagc
     4921 atttccggaa gcgtgcgcca taaaataggc aaccgccatc tggccggaca aaacaaacgc
     4981 ggcagggcgc gcaaacaggc ccaacaccag caaaatgccg ccgacaattt ctaaaatacc
     5041 ggcaagcagc aacagcccgc cgggcgaacc gctgcccatt tcaatgggga aggcgaagat
     5101 tttcgacgta ccgtgcaaca aaaacaggta ggcggttacg atacgcaaaa cagaaagcaa
     5161 aaccggttgg atacggttgc agcaatcgga cataaaagct cctttgatat ttgtcttcat
     5221 ttcaggcggt agagtataga tgtttcccct tttcaaatat atactgcttt tattgataca
     5281 acttttccac acaagaaaat atggacaccc tgttcagcct caaggttttc cgccaagtcg
     5341 tccaaagcgg cggcttcacc cgcgccgccg acgcgctcgg catctccacc gcaatggcaa
     5401 gcaaacacgt cagccacttg gaaaacaccg tccaagccaa actcctgcac cgcaacagcc
     5461 gcaacctcag cctgaccgga gccggggaag aatactaccg gcaatgcagt tacgcgctcg
     5521 acacgctcga cgatgccgcg caaaaagccg ccggagggac ggaaaaaccg caggggctgc
     5581 tgcgcgtaac gatgccgctg tggtttgccg gcagccagat atgcaactgg ctggcggaat
     5641 accgcgaacg ttatcccgaa gtggcattag aactgatttt ggacaaccgc cacgtcgatt
     5701 tgattgccga aggcgtggat ttggcgttgc gcgtttccca aaccctgtcc ccgtcgctga
     5761 ttgcgcgccc actggcggaa atcgaatttg ccctgctcgc ctcgcccgat ttcctgaggc
     5821 gcaacggcgt gccggaaacg ccggaagaag tggcggggct gcccgccgtc ctgccgacct
     5881 acaccaacca gcagaaactc gacctcaccc gcaaatcgga cggcaaaaaa taccggcttg
     5941 aactgacccc cgtcatccgt accgacaaca cgctgatgat gcgcgaaatg attaaggcgg
     6001 gcgcgtgcat cggttatcag ccgctttggg cggcggaaca cgatttgcgc tgcggctcgc
     6061 tggtgaggct gctgcccgaa tataccgtcc cgaccgaccg cctgaatgcc gtttatgcgg
     6121 acagggcgtt cttaagcgcg aaagtccgca gcttcatcga ttttctgaac gaaaaaatcg
     6181 ccagcaggaa aggctgccga aatgccgtct gaaaccgccc gcccccttta tgcggacacg
     6241 tgcgccgcac acacgctcgt ctggttccgc caaaacctcc gcatccgcga caacgccgcc
     6301 ttatgcgccg ccgttgccga aggtttgccc gttatcggta tttggattga cgatgccgaa
     6361 acagacaacc ctcgccgcgc cgcgttctac cgccaatccg ccgccgaact cgcccaaggg
     6421 cttgcagggc gcggcatccc gctctacacg gcggcatcgc ctgccgagct cgtccggctc
     6481 gccgtccgcc tcaatatccg cgccgtcatc gccgacgaat cccatacttt tgccgacaaa
     6541 ctcgccgaca acgccctttg gcatgaattg aacaaacacg gcatcgcgtt aaccttcgtc
     6601 aatgaccgcg ccgttttcgg caaaaccgac ctgatacccg acggcggcac ggcacacacc
     6661 gatttcgacc gctaccgcga agtatggctc gaccgctttt ccaagcagcc ccccgccggt
     6721 ccggacctat tcgcggcata ccgccaaccc ttccccgaaa acctttccgc cccgccgcct
     6781 gccgcccttt cagacggcat cttcctgccg caaaacagcg gcgaaacggc ggcttggcgg
     6841 cagtggcggc ggtttctcga acaggcggat tcctactccg ttttaaagga tttcccctcg
     6901 cgcaaacaca cttcgctgat gggcgcgtat ttgagtgccg gctgcatctc gccgcgcctg
     6961 ctcgcgcggg aaagcctcga acgccgtctg aacgcgtggg cggacaacat catccgccgc
     7021 gattttttcc ttcaacttgc cttgcagcac acggatgacg acccttcaga cggcaatcct
     7081 gaacacaccc tgcgcctgac gctttggcag cagggccgga ccggcattcc gattatcgat
     7141 gccgcgatgc gctgtttgca caaaaccggc agcctccacc ccgccctgag acgcttgagc
     7201 gcggattttt tctgccacgt tttaaacctc ccccgccgcg aaggcgagat atggtttgcc
     7261 cgacagctga ccgatttcga tgcagcaatc aaccaaggca actggcggct tgccgcctca
     7321 cggcacacct gccccgacat tgccgccgcc gcacacaaga ccgaccccga cggcaccttt
     7381 gtcagacggc acattcccga gcttgcccac ctgtccgccg acaccgtcca cacgccttgg
     7441 cggtttgcct gttcggtcga tacccacggc tatcccgccc atcctgtcgc cggtattccc
     7501 taagcggtac ggcaacctaa aacaaatgcc gtctgaactt ccgtttcaga cggcattgcg
     7561 tccgaatgac tcaatatctt ccgttttttt cactttcaat catcaggtat gcccaaatca
     7621 ccgccaccca agccgccacc gttcccatcg tcggcaaaaa atgctgccaa tccgtcatcg
     7681 gcggtacagg gcaacggccg cattcgattt ccgcgccgcc caacaaccgg tacagcagca
     7741 tatccgcaat acaaagcaac aacgggtatt tcaaacgctg caaccaaaaa accaaccacg
     7801 tccgcacgat ttacctcccc ccattccgac aacgatattg taccgcttcc gtgcaatgtt
     7861 aaaataaccc cacttctacc gccgtacaaa acgccatgct caccgattta gaaaaaaacg
     7921 ccatccgcga ccattaccaa aacatcggca aaaacctgcc cggtttccgt ccgcgtgctt
     7981 cgcagcggga aatgattgcg gcggttgcca acgctttttc gcggacgttg acgcgtgaag
     8041 aaggcgaaga agcacccaaa cgtgagggcg agagcattgc cgtgatcgaa ggcccgaccg
     8101 gcgtgggcaa atcgttggcc taccttttgg ccggcggcat catggcgcaa acgcgcggca
     8161 aacggctgat tgtcagcagc gcgacggtgg ctttgcagga gcagttggtt gaccgagatt
     8221 tgccgtttct ggtcgaaaaa agcggtttgg agctgacctt tgcgcttgcc aaagggcgcg
     8281 gccgttatct ttgtccgtac aaactctatc aactgacgca aaacaacgcc cagcaaaacc
     8341 tgcttggttt tgaagcgccg gaagtgttgt gggacagcaa acccaagccc gaagaattga
     8401 acctgctgcg cgacatcgcc gacgaatttt ccgcccgacg gttcaacggc gaccgcgaca
     8461 cctggccgga aaaaatcgat gacgcgattt ggctcaaagt taccaacgac cgccacggct
     8521 gcctgaaagc cgcctgtccc aaccgcgccg aatgcccgtt ttacctggca cgcgatatgt
     8581 tggaaaccgt cgatgtcgtc gttgccaacc acgatcttct gcttgccgac atcagtatgg
     8641 gcggcggcgt gattctgcct gcgcccgaaa acagtttcta ttgcatagac gaagcgcacc
     8701 acctgcccaa aaaagccctc agccgttttg ccgccgaaca ttcatggaat attgccgttt
     8761 ggacgctgga aaaactgccg caactgaccg gcaagattgc cgcgctgacc gataaagccg
     8821 aacttgccaa ccttgccgac gaagccgcct catccttact cgacagcctg cacgaatggc
     8881 agttccattt ggcggaagag ccgtctttga gcttggggcc gtctgaaaac gacaggcgta
     8941 ccaacagcga accgacttgg ctgtgggaag acggcaaaat ccccgaaggc ctcgaaacca
     9001 ccgtttccaa caccgccatt gccgcacgca gcctgctcaa acacgttatc gggctgaacg
     9061 atgcgctttc cgccgcgcgc cgcgaaaaag aacaagacag cgcgctcatc gaccgcctga
     9121 ccggcgagtt cggccttttt atctcccgta tcgaacaaat tagtgcggtt tgggatttgc
     9181 tctccaccgt tcccattgaa ggcgaagagc cgttggcgaa atggataacc cgccgtgccg
     9241 acgataaaaa cgactatatt ttcaacgcca gccccatcag cagcgcgtcc caccttgcca
     9301 acagcctgtg gcggcgggcg gcaggcgcag tgttgacctc cgccaccctg caatccttgg
     9361 gcaactttaa cctgatgctg cgccaaaccg ggctgcaatg gctgcctgaa accaccaccc
     9421 tcgccctcaa aagccccttt gactttgaaa aacagggtga actctacatc cctcccatat
     9481 acgccagccc caaagacccc gaagcccaca ccgccgccat catcgagtgg ctgcccaagc
     9541 tcatttcgcc cggcgaagcc atcggcacgc tcgtcctgtt ttcctcgcgc aaacaaatgc
     9601 aggatgtcgc cttacaactg cctgacgact atctgccgct cttgctcgta caaggcgaat
     9661 tgcccaaagc cgttctcctg caaaaacacc accgggccat agaagaaggc aaagccagca
     9721 tcatcttcgg actcgacagc tttgccgaag gactcgacct gcccggcacc gcctgcgtgc
     9781 aagtcatcat cgccaaactt cccttcgcca tgcccgacaa ccccatcgaa aaaacccaaa
     9841 accgctggat agaacagcgc ggcggcaacc ctttcatcga aatcaccgtc cccgaagccg
     9901 gcatcaaact catccaggcc gtcggccgcc tcatccgcac cgaacaagac tacggccgcg
     9961 taaccatcct cgacaaccgc gtcaaaacac agcggtacgg ccaacaatta ttagccggcc
    10021 tgccaccgtt taaaaggata gggtaaacct accgccctgc ccgcaacaga aaggaaaaat
    10081 catgaacttc acccgactgc tcaaccaagt cttaagcacg gttcaaaaaa aaggcaacac
    10141 cttctccgac agcccgctca attcattcgg cggaggcgcg ctggttgccg gtgtcgcctc
    10201 catgctgctg aacggtaaaa accgcaaaac catcaccaaa atcggttcga ccgccgcttt
    10261 gggctacctc gcctaccggg gctatcagat gtggcagcaa aacaaagggc gggcaaccgt
    10321 aacacaaagc gatttccaac ctgccggaga aaccgaagaa acatacagcc gcaccgtatt
    10381 gcgcaccatg atagccgccg ccgcttcaga cggcatgata gacgaagccg aacgccggac
    10441 tatcgaacag gaaagcggca cagaccccga aactgctgca tggctcgccg ccgaataccg
    10501 cctgcccgca agcatcgaag acatcgccgc cgccgtcggc aacgatgagg cgttggcggc
    10561 ggaggcctat ctggcggcaa gactggtatg tgccgatttg tcgcggaaag aaaccgtctt
    10621 cctcgcccgc ctgtcgcagg ctttggaact ggatgacaat ctggtggaaa acttggaaag
    10681 gcaattgggg ttttgattca aaattccctc ccgcagggga aggaaaataa aaaccgccgc
    10741 cgccccgaaa ggcaaacggc ggcacaaacg atatcggacg gcaaggcagg ctactgcaat
    10801 gcgccgtcgc gagaagattt aagcaggcgt tcgtgtttgt gaaattcccg ccccatataa
    10861 taagaaccga aataggtcag gtggtcttgg tcgccgtaaa gatagcggcc gtatatttcg
    10921 accgtgtttt tgggcaggta tttttgtgcg tccacccaat gcacattggg aatatcttta
    10981 atcaaatcaa agaccgcctg attgctcttg ccgatgtcgc ccatagcctg aatggggcgg
    11041 agatattggt ttgcggcaaa tcttttcaat ttttcctccc tcaggggcga acggctgatt
    11101 gatgtgttgt ttgcaaaaac atagacgggt ttgacggcgg ctatcctttt gacggtttcc
    11161 ctgaatcggg ctgggaaccc gggtattagg aaggattgcg cttcaaatct cggcacgggc
    11221 tggccgccca tcctcaaatc atagaattgg gcaatgaaaa cggcttcggc tttttcaact
    11281 tcatcccggt attttcgaca taacgggttg tctgccagct tctcatctac ccaaaccaaa
    11341 cactccgaat cgagggacag gattttggct ttccaccctt cccggctgcc gacataatcc
    11401 agaaaccccc gcaggtgtcc ggcgtgcgag tcgccgaggg tcaggacggt ttccggaaaa
    11461 tgattttccg cagcaagggg cgcgccgggc aacgggcgga ggtgttcctg tttcaatatc
    11521 ccccttgcgt acaggttgta accgacaagt atcagggacg gggcgagata gaggcagaaa
    11581 aatgcctttt tgaaggtcat cttccgtttt ctaagcggct gttcaatcaa ataataactc
    11641 aacagggaaa atccggccgt caacgcggca accgccgata cggcaggcag tccgagctgt
    11701 ttgtcgcctg taatgtaatg ggcgaaagca ataaaaatcc aatggtacag gtatagggaa
    11761 taagagattt tgccgacaaa tacgatgggg cttgccgaca ggatgcgggt cggaagtgtc
    11821 ccgtattgca tactccggat aagcagtgcc gtcagcaggc agggaaggag cagggtcatt
    11881 cccgggataa acggattgtg tttgtcaatc acgaacaggc aggcaagcaa tgcgccgaag
    11941 cagagtgatg aaagcaactg ccgttttcca tttgctgttt gccgtctgcc gttttgcgtt
    12001 tgcccgtaaa ccgccagcag cgaacctgcc aacagctcgg gaaacctcag tgtcgaaagg
    12061 taataagtat tgggttggtt gagaatatcg gtataaaacc cgcttggcaa aaacgatgtg
    12121 gcagtcaaaa tcagaaatag gatgatgctg atgttacgca gcacccgtag cgattttgtt
    12181 tttttgcagc aaaatatcag caaaagagga tacaggaggt aatactgttc ctctactgcc
    12241 aaagaccaga tatgcagtac ggggttctcg tcggcactca aatcgaaata cccctgctga
    12301 aaccccagat aaatattgga caagaaaacc gcagaaagct ccacggtttt ccgcatttgg
    12361 ttgaaatctt cgtaaaggaa gatttgagag gcaatcaccg aagccagcga cacggccgca
    12421 ataaaagcag gataaatccg cttaatcctg cgggtataaa aatcccggaa agaaaaagaa
    12481 ccgttctgta tttcagaaag aatgatgccg gtaatgagga atcctgagat gacaaagaaa
    12541 atgtccaccc ccaggaatcc tccgggcagc cagcggttat tcaggtggaa aatcatgacg
    12601 gatagcacgg cgacggcccg caatccgtca atttccggtc tgtatcggac agcttgcata
    12661 aatatcgccc ctgtatgtcc cgttatctta aaaatgccgt ctgaacgcgc gttcagacgg
    12721 catcggtttc agtaaacgct caaatccgtt tcgccaacgc ttcagccttg cccacataca
    12781 gcgcgggggt cagctcaagc aatttggctt tggcttcggc ggggatttcc agcaatccga
    12841 taaagccttt cagcacttcg ggcgtgatgc cgcctttgcc gcgcgtcagg tctttcagct
    12901 tctcgtaagg attggccacg ccgtaacggc gcattacggt ttgaatcggc tcggcgagca
    12961 gctcccaagt ggcatccaaa tcggcggcaa gcgcggcggg gttaggttcg agcttgttca
    13021 gaccgcgcag gtgggcggcg aaacccaaca cggcatagcc cacgcccacg cccatattgc
    13081 gtaagacggt gctgtcggtc aggtcgcgct gccaacggga aatcggcagt ttttccgcca
    13141 aaaaacccaa tacggcgttt gccataccaa ggttgccctc ggagttttca aagtcgatgg
    13201 ggttgacttt gtgcggcatg gtggaagaac caacttcgcc cgctttgact ttttgtttga
    13261 agtaccccaa agaaatgtag ccccaaacgt cgcggttaaa gtcgatcagg atggtgttga
    13321 tgcggctgag ggtttggaag aattcagcca tgtagtcgtg cggctcgatt tggatggtgt
    13381 aggggttgaa ggtcaggcca aggctgattt cgacgaagtt gcggcagtgg gtttcccaat
    13441 cgacgtcggg ataggcgacc atgtgggcgt tgtagttgcc gaccgcgccg ttgattttgc
    13501 cgaggaactc ttgcgcctga aggcttttaa actggcgttg caggcggtac acgacattgg
    13561 cggtttcttt gcccaaagtg gtcggcgtgg cgggctggcc gtgggtgcgg ctcatcatcg
    13621 ggacggcggc aaggtcgtgc gccatagcgg tcagtttttc gatgatttct gccagtttgg
    13681 gcagcaaaac agcctcacgc gcttcttgca gcattaaagc gtgggacagg ttgttgatgt
    13741 cttcgctggt gcaggcgaag tggatgaact cactcacggc ggcgacttcc ggcacttcgg
    13801 caaaacgttt tttcagccaa tattcaatgg ctttgacatc atgattggtc gtggcttcga
    13861 tggctttgac ggcggccgcg tcttccaatg aaaagttttc aatcaccgtg tcgatttcgg
    13921 caagcgtttc ggcactgaag ggcggcactt cggcaatctt cggctcggcg gcgagggctt
    13981 tgagccagtt taattcgact ttgacgcgcg ctttcatcag gccgtactct gagaaaatcg
    14041 ggcgcaatgc ttcaacggat tgggcataac ggccgtctaa aggggaaagc gaggcgatgg
    14101 ggttgatcat ttcggcatcc tgttcgggaa agacatcaaa aattgttaaa ttgtttgcaa
    14161 ttatacatca ctctcaggac gctatgccgt ctgaagcccg tgttccgaca gatacgcaac
    14221 gatttcgtct tcatcgtccg atatggcgaa caaagaggct gccccttcgg aaatcagacc
    14281 gcgcgccaaa agctgcgcgt ttatccactc cgccaagccc gaccaaaacg cctttccgac
    14341 caaaacaatc ggacgcggcg gcactttgcc cgtctgcacc aaggtcagga tttcaaacaa
    14401 ttcgtccagc gtcccgaagc cgcccggcat cacgacatat gcttgggaat agcggaaaaa
    14461 caccgccttg cgttcggcaa aacgggaaaa ccgcaaggcg atgtcctgat acggattcgg
    14521 tttctgctcg tgcggcaaaa cgatgttcag ccccaccgaa accgacttcc cggcaaacgc
    14581 gcccttgttt gccgcctcca taatccccgg cccgccgccc gaaatgacgg caatgcccga
    14641 atccgacagc cgccgcgcca gacggcagga gaacgcataa tccgcatgat tctgcggcgt
    14701 gcgcgcgctg ccgaagatac tgactgccgg aaacacgccc gccaatgctt cgtctgcctg
    14761 cctgcgttcg gcatcataac gtgcctgctc cggcacacgg tttgtattct ccattccatc
    14821 ctccgttcaa aaacagcgat tgtacaccgt caaaaa
//
LOCUS       DAWKOD010000037        14476 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000037 DAWKOD010000000
VERSION     DAWKOD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.037"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..427)
                     /locus_tag="WJK73_000839"
     CDS             complement(53..427)
                     /locus_tag="WJK73_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557351.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(600..956)
                     /locus_tag="WJK73_000840"
     CDS             complement(600..956)
                     /locus_tag="WJK73_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ7557352.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTESLVKNIIH
                     ALEYAIKNEKTVIFHF"
     gene            complement(1099..1443)
                     /locus_tag="WJK73_000841"
     CDS             complement(1099..1443)
                     /locus_tag="WJK73_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557353.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1602..2003)
                     /locus_tag="WJK73_000842"
     CDS             complement(1602..2003)
                     /locus_tag="WJK73_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557354.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2007..2747)
                     /locus_tag="WJK73_000843"
                     /pseudo
     CDS             complement(2007..2747)
                     /locus_tag="WJK73_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2817..3134)
                     /locus_tag="WJK73_000844"
     CDS             complement(2817..3134)
                     /locus_tag="WJK73_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557355.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3131..3733)
                     /locus_tag="WJK73_000845"
                     /pseudo
     CDS             complement(3131..3733)
                     /locus_tag="WJK73_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4159..4503)
                     /locus_tag="WJK73_000846"
     CDS             complement(4159..4503)
                     /locus_tag="WJK73_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557356.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4541..4864)
                     /locus_tag="WJK73_000847"
     CDS             complement(4541..4864)
                     /locus_tag="WJK73_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557357.1"
                     /translation="MFLDDVNIFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(4867..5055)
                     /locus_tag="WJK73_000848"
     CDS             complement(4867..5055)
                     /locus_tag="WJK73_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ7557358.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(5125..5469)
                     /locus_tag="WJK73_000849"
                     /pseudo
     CDS             complement(5125..5469)
                     /locus_tag="WJK73_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5553..5855)
                     /locus_tag="WJK73_000850"
     CDS             complement(5553..5855)
                     /locus_tag="WJK73_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557359.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(5878..6147)
                     /locus_tag="WJK73_000851"
     CDS             complement(5878..6147)
                     /locus_tag="WJK73_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557360.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(6168..6374)
                     /locus_tag="WJK73_000852"
     CDS             complement(6168..6374)
                     /locus_tag="WJK73_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557361.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WJK73_000853"
                     /pseudo
     CDS             complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WJK73_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(7195..7566)
                     /locus_tag="WJK73_000854"
     CDS             complement(7195..7566)
                     /locus_tag="WJK73_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7557362.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(7563..7814)
                     /locus_tag="WJK73_000855"
     CDS             complement(7563..7814)
                     /locus_tag="WJK73_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557363.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(7946..8152)
                     /locus_tag="WJK73_000856"
     CDS             complement(7946..8152)
                     /locus_tag="WJK73_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557364.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(8162..9007)
                     /gene="mafB"
                     /locus_tag="WJK73_000857"
                     /pseudo
     CDS             complement(8162..9007)
                     /gene="mafB"
                     /locus_tag="WJK73_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(9104..9316)
                     /locus_tag="WJK73_000858"
     CDS             complement(9104..9316)
                     /locus_tag="WJK73_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557365.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(9392..9751)
                     /locus_tag="WJK73_000859"
     CDS             complement(9392..9751)
                     /locus_tag="WJK73_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7557366.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(9756..10472)
                     /locus_tag="WJK73_000860"
                     /pseudo
     CDS             complement(9756..10472)
                     /locus_tag="WJK73_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(10689..11072)
                     /locus_tag="WJK73_000861"
     CDS             complement(10689..11072)
                     /locus_tag="WJK73_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557367.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(11038..12483)
                     /gene="mafB"
                     /locus_tag="WJK73_000862"
     CDS             complement(11038..12483)
                     /gene="mafB"
                     /locus_tag="WJK73_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ7557368.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(12523..13485)
                     /gene="mafA"
                     /locus_tag="WJK73_000863"
     CDS             complement(12523..13485)
                     /gene="mafA"
                     /locus_tag="WJK73_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7557369.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(13669..14388)
                     /locus_tag="WJK73_000864"
     CDS             complement(13669..14388)
                     /locus_tag="WJK73_000864"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ7557370.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
BASE COUNT         3950 a         2944 c         2573 g         5009 t
ORIGIN      
        1 cgagtttatc ctagttatcc aggatggtcc catctctttt atagatagct gcttaccaag
       61 tatccttaat tgctaattca agccactctt caaaactatt aaaaaattca tggttttcta
      121 tattatctaa attaatgaca ataaccctag gatttcctgt tacatcatta ccatcaaaat
      181 aagcttccca ttctccatta ctagcgaatg ggataagatt taaatcaggc aggatagaat
      241 ttctaaatat ataagctact tctgcccctt ctgttccata attctcaaac caccagtgaa
      301 aattatgtgg ctgaatataa ggtattaaag atgtatcgtt agagatacga acatactttt
      361 caggataaca aaacctttca ggaagcatat tactaggata taaattaaat tcattttgaa
      421 taatcatttc gttaccttag gatcataaat actaccatta ggaaactggg tagaccaatt
      481 tggtggtgct agttcatttt caatttgttt tttaattcta aattgaatag cttctcccca
      541 tgtttgcggc atctaatgct gcttgctctg catcaatatt actaaaaatt ggattagtct
      601 caaaaatgaa aaattactgt tttttcattt tttatagcat attcaagtgc atgaataata
      661 tttttcacta aggactctgt agaaaaatct gaattttgta atttttcttt tacagccaaa
      721 atactttcct tatctattag ccaatccccc tcacttggtc caaataacca attatcggta
      781 atttcagaaa caagtctcca taatccacga ttttgatatt caataatttg attttccgta
      841 atttctacaa ctgtttcaga ttgaatgttt ttataagccc aatcaatcat ttcagtatat
      901 tcactaattg aaacattgtc ttttaaacag aaaattaaca ggttgtgttc attcatttgt
      961 ctaagttcct gatatgagtt tgttgttgta aataatcttt tgcttgctta ccttgtaaat
     1021 taaaggatta accgcacctt ggatgatgcc gcgaatatca tccatacggt taccccacca
     1081 atttttattg gtgtatcgtt aatcatttat ttcaaaaaag ttatttatta tcatattatc
     1141 agggagcagg tcgttaaaaa aaaccccctc aaattccatt aaattagtat ttatccagca
     1201 cataaacgaa ccaaagcgtt ctttccctga gtgtaaatat ttaaaattaa actcaaaata
     1261 agtttttttg cttagaaaat ttaatggttt ttctatagtg aaaaaaaagc ccttactcgt
     1321 tttcgttcga gatactattt taaataaatt tttattttca ataaaataag aatattcatt
     1381 tactaagttg ttatgataaa aaagtaattt tattaaaatg ttttcataat tttctaggtt
     1441 catttctagc tcaatttaaa atgatttcat tgttgccgta ccaatgcgaa atttaccatc
     1501 aacaaaattt cccttcactg atacaggtac accatcaact tgggtagttt tattttcagg
     1561 ctgcctgaaa gaaagatagg caacttatta ctcatcttat gctagccctc aataattgaa
     1621 tacgttgcac tagaaccaat tatttctatg aatgtttgct caaacataaa aatatagtat
     1681 ttaaagaatt ctctattcaa aggaaaataa ctttctttca atatccattc cgcataacac
     1741 tcatcaagaa gttcaaaaaa agattgacca ccattttcat gaagcaaagc tctatctgca
     1801 tcttccgaaa ttaaatccat aaaagtatct ataaaatacg attcatcaac aaccctaaaa
     1861 tgaataacat cttcaataaa aatttttatg gttttatccg tatttctata accaactgct
     1921 ataacttcta ctttatcaga gtttattgct gcattatcaa aatagaaata gccagttggg
     1981 aaatctaagc tttttaattt cataatttat cgtgtatttc catatctgat ttttattttt
     2041 cggttattac taattgttat ttcaagtgtt ggttctccac cactactatc attacgagca
     2101 ttaaccgcac gcccatcagg taaattgccc gttatcgttc cattaggacg tgtttgtaca
     2161 tttcgaacat tcagactatt aaaatcattt agtgcgtcat tgtaatcccc cactttatca
     2221 tactgaacag cgggaccttt tgttttactc ctttgagtag tattgctcaa aatgtctttg
     2281 atttgtaaat cactgcgcgc cttaccggct tctatgcgag ctgccacact actcgggcta
     2341 taggggtcgc tatcaatcgc aggattacca ccgctggttt tttgtccgct agcctgtttc
     2401 ccaacctgct gtactgtatc gccaatttca tctaccgtac ctaacgcctt agtctgcata
     2461 tggcgaacag caggtttttt atagccggta tttttaaccc aatcccattt ggtcgggtta
     2521 agttctactt ttttacctct ccaaaccgta cctgcggcct ctgctacggc aagggcagtt
     2581 tgggctgttg ctgttatatt cggatgggca tcagcccatt gtctggcgga attgatgccg
     2641 tcttttaccg caaaagcact gtcctgtaat atgctcgcgg cagcaagttg tgcttccggg
     2701 cttaattttc ctaaattatt aataccttgt agagtctgct gcgcagagat gtagcaactg
     2761 tatttttacc ccgtcgggta aaaatacagt tgctacggct gctgggaaag cagttactat
     2821 tttcccagtg gctcaaaatt aattgtttca gcagatattt ttataataat ccctacatca
     2881 ataataattt cactataaaa ttggtattta tatagctcta tttcgggtat aaacaaggga
     2941 aacaagctat cttccttatc ctcataatct acgaaattat ttgtatctaa tttaaaattc
     3001 aaaatattac tgcatttgat atatccgaaa taattacctt caattgtatc tgtaaaagat
     3061 aatgtaaggg tatttccatt atcggaaaag attatttcat aaaggcttac attgtgaaaa
     3121 atattttttt tcatttgata aaatatccag aatgatgacg attatttggc aatggattgc
     3181 cttcataatt ttgtcgttgc ggatattttc cctgtacacc atcggttcgc ggcactcttt
     3241 gggttcttat aacctctcca tttggacgaa tatctatttt cacgccatca ggacctttcc
     3301 atgtttcata ttgagcctca tctgaaccag tacgtctaaa accttgttct tgcaatgctt
     3361 gacgtaattc atctcgcgta gttggtctga tagatttaac aactggcttg tttcctcctg
     3421 ccatctcacc gtccaacgtc tgcatagggc gggcagcagg tgttttatag ccgttatttt
     3481 taacccaatc ccatttagtc gggttaagtt ctactttttt acctctccaa accgtacctg
     3541 cggcctctgc tacggcaagg gcagtttggg cagtagctgt aatgttcgga tgggcatcag
     3601 cccattgtct ggcggaattg atgccgtctt ttaccgcaaa agcactgtcc tgtaataggc
     3661 tcgcggcagc aagttgtgct tccggactta attttcctaa atcattaata ccttgtaaag
     3721 tttgctgcgc agagatgtag gtcggatatt tgtatccgac aaaaatattt gacgcatcta
     3781 tcgtttccga aatgccgctg ttggaaaatg tcggattcga gaatccgacc tacggctatt
     3841 tcgtttaagt cataaatagc cattcatgtt ccttctgaat ttgcttttgc tatccttaga
     3901 accaatcata taaacattat catcattaat taatgttcta agtttatgaa gcggtcaaat
     3961 ctgtcaaact accagagatg aagcatatcc tcctggaagt aattctgttc gtgctgtttc
     4021 catatcttct ttgccatcat cagttacttc tctgtctaaa taaaatctta cgataagttt
     4081 ttgtctaatt tcatcaaatt ctgccgtaat taatcttata ttgatatgaa tacacggttc
     4141 atacctgcta ttagccagtc aggtgtagtt ggatggggat tcatttttaa taatatcttc
     4201 aagcaagtga aatgtaagaa aagcgtagat acttgaaggc cggctatctg attggcaaaa
     4261 agaaatttcg gagataaaga atttccgttt atatttttca taaattaaat caacaccttg
     4321 ttttacataa aaactaactt cctcaggaag gatatttata acattctctc cttttacttt
     4381 tatagttaat ggtttgattt ttatgttttc atgaatatct gataggatta tttctaaaaa
     4441 attattttta tttccagtca ttctggctat tttatgtata tttccattgt gttcgattat
     4501 cattttattt tttccaagga tttccttcta tattgatagt ttatctaaag atttcattta
     4561 aaatatcctg actaatttct tgtttatata attctacaat tttctgtttc tgtgtatttg
     4621 tataaaattt ttcgttggta tctgcttgat attttaattg tctcaatatt tccatgattt
     4681 catattctga tttttcatca tattcttcaa tcatgtaatc aacaaattgt tttagaatat
     4741 caaaggcttc gtaactttcc aaaattttaa tatgtttatc ggcaaaattg gacatatacc
     4801 attcgtcagt gattggatta gtatttaaat catctaaaaa aatatttaca tcatctaaaa
     4861 acatgactaa tccttaaact taaaatctgt tttctcaccg gttcttgtgt tttcaatgta
     4921 atgtatttca attttgttta cgttttgaac acgcttaacc caaccgtctt ttgctaacca
     4981 accattttta gtatcggaca taggaggtat tctcgcagga gttttgccct gtgggtttgc
     5041 cttaacttct tccattgcta attgttcctt taaattatta ggtattgtac gaccggttga
     5101 tttttcaaca gattgtgtcc ctatttttaa tgatttgttt cccccagcca tttccccgtc
     5161 tacagtctgc atagggcggg cagcaggttt tttatagctg gtatttttaa cccaatccca
     5221 tttagtcggg ttaagttcta cttttttacc gcgccaaacc gtacctgcgg cctctgctac
     5281 ggcaagggca gtttgggcag tagctgtaat gttcggatgg gcatcagccc attgtctggc
     5341 ggaattgatg ccgtctttta ccgcaaaagc actgtcctgt aataggctcg cggcagcaag
     5401 ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct gctgagcggc
     5461 tgtatctgag ccggtcagtg taggttggat taaaagccag cattttcaac atcttgatgt
     5521 tttactataa atctattctt gtgcttcctt tgttagataa atttcaacac aaaagatacg
     5581 tatgcattct ctaattttat ttgagataaa taatgcattg ctttgtcgat actgttaaat
     5641 aaattacttc cctcgtaata ccaattatca tatgtttgat aaaaataatt atctttcttg
     5701 atataaatat ctccccccaa tacaagtatt ctctcattga taaataaatt taatatatct
     5761 attgcaatag ggacgggtaa tgcggcttct ctaattccaa ttcctatcaa agattcccct
     5821 tgtctcgaat aaatttcaaa aatttcattt aacatgttaa tctctatcta ctatttttca
     5881 atcggtataa accctattca cagtttcatc ttgtatttct ttatgaatac ttaatatatt
     5941 aagatagctt aggaatttta ttttctcaga ttgaggcaac tcatcaaaac caagccaaca
     6001 ataaaaattg ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc
     6061 ttcgactaac tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga
     6121 taggtaacca tgggattccg tcatcattga tttctctagg tgcttattta tctgattggt
     6181 aagccttttg cgtaagcttc tttcgcctcc gcaagtgtca tttcattagg gccaccatct
     6241 atattagttt ctcctgtatt ttgccactga caaacgtcac agatatcata gtccataact
     6301 tcagttccgc aaacaggaca atgtagccat atttctccgt caatttccca tgtctttttt
     6361 gaattctcca tcataatact tctttcctaa gtttggttta aacattgttt tgattttgaa
     6421 tgtttttgga ttggccaaaa catagatatt gttattgata tcatagcgca ctcgtctatg
     6481 ttccgtctga taacctaata tcttgtccga tgtctgtttt aataacaaat cagacgccat
     6541 tttttggtag tcttcaatgg ttatatcgcc aaattctttt ccatgagctt tgaaatgtcc
     6601 atttaaagat ttctcagtag gaaacttgga ctttgtccat ctgatgcggt cttgaattgt
     6661 tcctgtcgga gaaatagatg tatcaggttt aacatatgag gcaggtaagc cgatattgtt
     6721 atcagagtgt gtagggtgtt gctgcgtact tggttttggt ggcttattcc cacctgccat
     6781 ctccccatct aaagtctgca tagggcgggc agtaggtgtt ttatagccgg tatttttaac
     6841 ccaatcccat ttggtcgggt taagttctac ttttttaccg ccccaaaccg tacctgcggc
     6901 ctctgctgcg gaaagggcag tttgggctgt tgcagttata ttcggatggg catcagccca
     6961 ttgtctggcg gaattaatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcac
     7021 ggcggcaagt tgtgcttccg gacttaaatt tcctaaatca ttaatacctt gtagagtctg
     7081 ctgcgcagag atgtaggtcg gatatttgca tccgacaaaa atatttgacg catctatcgt
     7141 ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctgctacca
     7201 accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct cgtaatattt
     7261 tgtattatct aaattaacta ctataatttt aggattacct gatttatctg aaatatcaaa
     7321 acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc cagttatttc
     7381 aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc accaaggaaa
     7441 agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta aataactttg
     7501 cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac tattttgttt
     7561 tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct tttgctccac
     7621 ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat agcttctccc
     7681 caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga tgataatcgg
     7741 tcattaaaat tatgaacatt cgcttccact tttactcctg cttcacgtgc tgccataata
     7801 cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc aaccctgtcg
     7861 ccaacccatg atttttttct ttcatttcgg ctacaataga atcatagttg taatttgcac
     7921 taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt ccagcaaact
     7981 gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca aattctcttg
     8041 aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca aaaacttcat
     8101 cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc atggcttttt
     8161 cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta cctccatcgt
     8221 cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac gcagttctac
     8281 cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata tgccgagcaa
     8341 aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg acatgcttgt
     8401 tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa gacggttgtg
     8461 tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc ccaccggcca
     8521 tttccccatc caaagtatgc atggtgcgaa cagcaggtgt tttatagccg gtatttttaa
     8581 cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc gtagttgcgg
     8641 cctctgctac ggcaagggca gtttgggctg ttgctgttat attcggatgg gcatcagccc
     8701 attgtctggc ggaattaatg ccgtctttta ccgcaaaggc actgtcctgt aataggctcg
     8761 cggcagcaag ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct
     8821 gctgagcggc tgtatctgtg acagggctta ccgcactgtc tgtaattgcc ccaatcccta
     8881 ctccttgaaa acccattaaa aaaggattaa ccgcaccttg gacgatgccg cgaatatcat
     8941 ccatacggtt agcccaccaa tttttattgg ggtcgttttc ccatatcagt tttcctgctt
     9001 catcagcaca gttaggattg gcattgatgc ccacccagtt tcaggcagcc tgaaaaggat
     9061 ttttaatata cggcaaaagg tgggcaacaa gtgcccacct tacctagcta acatttatcc
     9121 caagtatcat atattttttt aagttcttta tccaaagaaa gaactttatt ttttcaacac
     9181 catgaattat ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt tctcctacaa
     9241 ttgcttgaat aatatacaac tcatctttat cgagactaat tttaactgaa ctctcttcaa
     9301 aattaattaa tttcatgaat aattcctttt attttaattc gtcatcaaaa taccgtttgc
     9361 ctgtttttgg tctaaatgct gtccaccatc tttagtcttg gatatttaga ttataatcgt
     9421 caaattccca gtcaaaatat tttgccaaat ttgatataga cttatcttca gacgttgaat
     9481 tacatatttc caatatatca tttatcaatt ttaatatgtg ttcaatatta aaacggtttg
     9541 taacaattga gttttttaat gacatcggtc tatgtatatt tgaaagtaac cattcagtgc
     9601 tatatatata aacataaaaa taatcagcac cattactttc atcaaaacct attgataatg
     9661 tcaaaagtat acaaaaattt tctaacttgt cttgtgggaa atcttccaaa tcaaagtcag
     9721 aacaatcata attttttaat tttaatctca tataattacc taatatttaa atgtccattt
     9781 ttaattttat tcctttttcc atttgaatca atagtataag tttcaaaatt tgcttgaata
     9841 cctgtagttg caaattgtga atttttttct gttggtggcc gatattgtcg agtgccatct
     9901 atacttaacc atccgcctcc actagtttgt cttgcaccct caccaaccca aattttacct
     9961 agcctatctg cctcttcata agttgcagtt cctattggaa aatttttttc actcttatga
    10021 agagctagac tcaatcttgg atcttgagcc gcaatgtttt ttaagttttg cccagttaat
    10081 tgattaacta attgaggata agttgcagca ttggcttttc ctccagacgt tatagattta
    10141 ggcggtttat tcccaccggc catttcccca tctaaagtct gcataggtcg ggcagcaggt
    10201 gttttataat cggtattttt aacccaatcc catttagtcg ggttaagttc tactttttta
    10261 ccgccccaaa ccgtacctgc ggcctctgct atggcaaggg cagtttgggc tgttgcagtt
    10321 atattcggat gggcatcagc ccattgtctg gcagagttga tactgtcttt taccgcaaaa
    10381 gcactgtcct gtaataggct cacggcggca agttgtgctt ccggacttaa atttcctaaa
    10441 tcattaatac cttgtagagt ctgctgcgca gagatgtagg tcggatattt gtatccgaca
    10501 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
    10561 aatctgacct acggctgctg actattttct tgtttatata attctacaac tgcaaactac
    10621 tatcaaatct ttttatctct ggctctacac tcagcaagaa tatttttatt ttacaaaaca
    10681 taggctagtt atttgagtat attgtcaaat tctaatttgt ttttttgata gatttttttt
    10741 ataccattaa tacattcatt agttgtaatt ctaccaagcg aatgtaattt atcaatatca
    10801 aaacaatcgg tataaaccct attcacagtt ttatcttgta tttccttatg aatacttaat
    10861 atattgagat agtttagaaa ttttattttc tcagattgag gtaactcatc aaaaccaagc
    10921 caataataaa aattgttata tgattttaaa aaatcaacaa tttttatttt tcctaaaacc
    10981 aaatcttcaa ctaactgggt tgcataaata atatcattta tgattttaac aggtaaatta
    11041 tttgatgggt aaccatgggg ttccgtcatc atttgtaact cctggtacat gtgcgtgtcc
    11101 tgtaagtgcg cgtgggtctt gatgggtatt tgcatgatga gcatcatcta ttctggctcc
    11161 agtcaagttt ccattactat ctcggacttg gataaaacga gcatcaggcg aacctgttat
    11221 ataaccgcct attggtgttg gtattgcatt tcctttgctg tctacataaa ttgttcctgt
    11281 cggagaaata gatgtatcag gtttaacata tggggcaggt aagcctatat tgttatcaga
    11341 gtgtgtagag tgttgctgcg tacttggttt cggtggctta tttcccccag ccatctcccc
    11401 gtctacagtc tgcataaggc gaacagcagg ttttttatag ccgttatttt taacccaatc
    11461 ccatttggtc gggttaagtt ctactttttt acctctccaa accgtacctg cagcctctgc
    11521 tacggaaagg gcagtttggg cagtagctgt aatgttcgga tgggcatcag cccattgtct
    11581 ggcagagttg atactgtctt ttaccgcaaa agcactgtct tgtaatgcgg ttgcggccgc
    11641 aagttgtgct tccggactta aatttcctaa attgtgaata ccctgtaaag ttttccgtgc
    11701 tgccgcatag gttaccgggt ttaccgcact gtctgtaatt gccccaactc ccaatccctg
    11761 aaaacccatt aaaaaaggat taaccgcacc ttggacgatg ccgcgaatat catccatacg
    11821 gttagcccac caatttttat tggggtcgct ttcccatatc agttttcctg cttcatccgc
    11881 acggctgaaa aaaccagagg cggcaccggc attttctttt agccaacggt ctgaaaatgg
    11941 ggcttgaggg acaatattag tctttgtttt tgttgaagtt cctttgacat aatagctgta
    12001 tatatccctt gctcctccgg ggggcggata atcgctgcct tgcggcccgt catatccatc
    12061 ctccggatga atttccgacc ctgtccgatg aagttggtaa acagtaaaac cgccgtctac
    12121 accgccgctg aaatcagaag tgctttttga atcatgatta tcgaacggac tgtgtacttc
    12181 atgtccatgt cctgaaaaat gggtttcata accgataatg ccttcaaacc ctgtccgttc
    12241 gtgtgtaata ggcagtacag gactgaccgc agttgcatca aatgtctgga cggcgtaaac
    12301 ccgattttta acactgccgc gagcattacc aaacagatgg tatttaccac ccggttcgta
    12361 gtgttttgcc tgcatatcat cgcgtaaacg ggcatccaaa ccgttcgcat gactaatggg
    12421 gatttgcagc aacgatattg ccgctgcaaa cagcatcatg aatttttgaa taggcaattt
    12481 cattttgttt tccttggtag caagcggtta tggtcgtgtg aatcaaggtt gcccttgtct
    12541 atgttgtcgc actacttcat cgctgtatcc atacccctca tgactgttat cagtctctac
    12601 ggatggggcg gagttacccg tatgattgcc gtatggtcgg atatcggaga aatcgaccat
    12661 taatccttcc gtcggtttaa ttcctttgct tactttatac ggccccatcc acaatgcgta
    12721 attttcttta taggcagctt caaacgcatt ggttttgggt ttgatgagca attttttatt
    12781 ggttctgtct actgcgaaat attccagttt tgtttgggct ttcagtgttt cggcattgta
    12841 taggtgcatt tcggttctgt tgcgtatcgt tccgaatacg tcgatgttaa taaacacatc
    12901 tgtatcggca ttggcaggag aaacaacgtc tatgccgcgc aggaaaaata cggtctgtac
    12961 caagtgggaa agaaaggcag tgtcgcgcgg gttagtcgtc aaggtttcat ttcgataatc
    13021 ccccatcccg ccaatattta agcccagact gctcctactt ccgctaccgt ctgattgggt
    13081 gcgcgagcgt gcaggggcat taagtgtaga taaagaagtg gttaaacctg tcaaaccgcc
    13141 tgatgttgtt tcagcggtgg tttcgtaacg tggataggtg taatcggtac ggacggcagg
    13201 gctgtttatg tattcgccac gaatcagtgc atcaatggag tagcgacccc ctgtcaaact
    13261 gcctgaacct tggtcgccca tagtggcaat gtacaatgca acttttcgtc cgtgtaatgc
    13321 ctgtaaatcc atgtctttaa cggcagctct ggcagaagcg gccacaagtt cttgttcgac
    13381 cgcaaagcgt ttacctccgc catgcgatgg aatacctgtc agtgtcccgc aggcggataa
    13441 aataaaaact gaaaaaagaa taggtatcag cagccgtgct tgcatagatt ttctcctttg
    13501 atgaaaaaca aattgtatca aaattgtaaa tatagtggat taaatttaaa ccagtacggc
    13561 aaggcgaggc aacgccgtac tggtttaaat ttaatccact ataatctcca tataataaat
    13621 ggctgtctga agcccatatg cgactatatc tgccgacact atggtcaatc agcagtgaac
    13681 cagcgtacct tcgttttcac cggttactac acgtttgagc gagccttctt tagcaatgcc
    13741 gaatactaca atattgagct tgcgttcgcg gcagagggcg aaagcggtgg catccatcac
    13801 tttgaggttt ttcaacaagg cttcgtcaaa agtaatggtt tcgtagcgcg tggcggatga
    13861 gtcttttttg gggtctgcgg tgtacacgcc gtcaacgttg gtggctttga gcatcacgtc
    13921 gcagttcatt tccgcaccgc gcaatgcggc ggcagtgtcg gtcgtgaaga acgggttacc
    13981 ggtaccggcg gcaaaaatca cgactttgcc ttcttccaaa tattggatgg ctttaggacg
    14041 ggcgtaagtt tcagcgattt gctgcataga cagtgcggat tgtacgcgcg ctttgatgcc
    14101 taaagtttca aatgcgtctt tgagtgccaa cgcgttcatc acggtcgcca tcatgcccat
    14161 gtagtcggcg gtggcgcgat ccatgctgcc tgcttgggcg gatacgcccc ggaaaatatt
    14221 gccgccgccg acaacaatac cgacttgcac gcccatttta acgacttcgg caatttcgcc
    14281 gacagtttga acgatggtat cgtgattgat gccgaacgga tcggaaccca tcagggattc
    14341 gccggagagt ttcagtaata cgcgtttgta tttgatttgc tgtgtcatgg gataccttgc
    14401 tttcttgaat gtcgtcggtc ggagtttggg ccgtctgaat tttcaaacgg tcggaattac
    14461 aatgatatgg tgtgtc
//
LOCUS       DAWKOD010000038        14429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000038 DAWKOD010000000
VERSION     DAWKOD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.038"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(338..4507)
                     /locus_tag="WJK73_000865"
     CDS             complement(338..4507)
                     /locus_tag="WJK73_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ7557371.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WJK73_000866"
     CDS             complement(4562..6409)
                     /locus_tag="WJK73_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ7557372.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMLEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WJK73_000867"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WJK73_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ7557373.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WJK73_000868"
     CDS             8315..9808
                     /locus_tag="WJK73_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7557374.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHMENGRPSPSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9883..10098)
                     /locus_tag="WJK73_000869"
     CDS             complement(9883..10098)
                     /locus_tag="WJK73_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557375.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLKAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(10091..10307)
                     /locus_tag="WJK73_000870"
                     /pseudo
     CDS             complement(10091..10307)
                     /locus_tag="WJK73_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WJK73_000871"
     CDS             complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WJK73_000871"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ7557376.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11850..12650
                     /locus_tag="WJK73_000872"
     CDS             11850..12650
                     /locus_tag="WJK73_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ7557377.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYLGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            12690..13676
                     /locus_tag="WJK73_000873"
     CDS             12690..13676
                     /locus_tag="WJK73_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ7557378.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14333..14429
                     /locus_tag="WJK73_000874"
                     /pseudo
     CDS             14333..14429
                     /locus_tag="WJK73_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3080 a         3649 c         4335 g         3365 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttaatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc accgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ttcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcgacggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggcacgaa ggcggacgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcagccttca
     3901 ggtcgaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttgtcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgaac
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg tggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgag catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaactaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggcg aaccggcaaa aggcgaaatg cttgcgggca cggccgtgta
     9481 caacggcgaa gtgctgcatt tccatatgga aaacggccgt ccgtccccgt ccggaggcag
     9541 gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgttatc gatggaaacg gctttaaggg
     9661 gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga
     9721 agaagtggcg ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ctgatgccgc ctgaagcggg ggcgttcaga
     9841 cggcattgtg atgtttgggg tggggtccgg ttcaagtccg catcagttga taaacccgtc
     9901 gtctttcatt ttcttttcgg aaaaatactg gccgaatacc agcagggcag cgaggatgat
     9961 gacaaacagt cccgccatag tcagagtgat tttgaaaaac aattccgctt tgagaaaaga
    10021 aaaccagagt tgcacaatca gcagcagcac ccagccggca aacagtccga gacaggcata
    10081 aacgccgagc ttaaacacga tcccgctcca aaatgacgac gtaggcctcg cgtttgaaaa
    10141 acagcagcat acggcgcaaa tctttttcca tcgttacgac ccgccagcct tcgggggatt
    10201 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    10261 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatagg attcctttta
    10321 acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgtaa aacagcaaac
    10381 cttattgctt attgcccaac tgcggcaata ccgaaccttc gcccagcgag agcaaaccgc
    10441 tttgcgagta gatacccagt ttgacgcggg tgtcggtgat gtcgaggttg cgcatcgtga
    10501 gctgtccgat gcggtcgagc ggagtgaacg cagcgtcttc gactttttcc atactcaggc
    10561 gttcaggttg gtaggtcagg ttgggcgatt cggtgttcag aattgagtag tcgttgccgc
    10621 gccgcagttc gagggtaact tcgccggtaa cggctttggc aacccagcgt tgggcggttt
    10681 cgcgcaacat cagggcttgg ctgtcaaacc agcggccttg gtagagcaaa cggccgaggc
    10741 gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggatgccg gtcaccaagc
    10801 gttcgtaggc gatgtggaac aacgccattc ccggggcttc gtagatgccg cgcgatttgg
    10861 cttcgatgat gcggttttcg atttggtcgc tcatgcctaa gccgtggcgg ccgccgatgc
    10921 ggttggcttc gaggaagagt tcgacggggt cggcgtattc tttgccgttt aatgcgacgg
    10981 gcacgccttc ttcaaagcgc acgctgactt cttcgggttc gatttcgacg ttttcgtccc
    11041 aaaaggcaac gcccataatg ggtttgacga ttttgatgcc cgagttcaaa aattccaagt
    11101 ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggagtaggct ttttcaaccg
    11161 acattttgta gttgaagccg ttggcaatca gaaattcgct catttcgtga cggccgccga
    11221 gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg ggattggtga
    11281 gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg ccgtcgcccc
    11341 agatgtttac gtcgtcttct ttcattgcgg aaacaagcat cgtgccggtt acggcgcggc
    11401 ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga aacgtggaac gcgccgcatt
    11461 ggatggcggc gatgccttcg tgtgccaact gcgcgcggca gtcgattaag cgggcgtttt
    11521 ccgcaccgta ttccatcgct tttttgggaa tggcgttgta gtcgtcttca tcgggctgac
    11581 cgaggttggc agtgtaggca taaggcagtg cgcctttgag tttcatccac aacagcgcgg
    11641 cggaggtatc aagtccgccg gagaaggcga tgccgacttt ctgaccgacg gggaggtttt
    11701 gtaaaatagt atggttttgg ttcatgaaaa aatcctcggt agcttgaaaa atcaaagcca
    11761 ttatcttata aataggaaat attgtcagcc atcatgccgt ctgaacgggt attccgccgt
    11821 ttcaggcggt atttaaaacc gaaaacatta tgaacgcgtt caaccttatc atcatcggcg
    11881 acgaaatctt acacggcagc cgccaagaca agcatttcgt ctttttcaaa tccctgctcg
    11941 aatccaaagg gctgaagctc aatcaggtgc aatacctgcc cgacgaacct gatttgttgg
    12001 tcaaacaact gcgccgcagc ttttcagacg gcatgccaac tttcgttacc ggcggtatcg
    12061 gcgccacgcc cgacgaccac acccgccaag ccgcagccgc tgctttggat ttgcccgtcg
    12121 tccgccacgc gcaagccgcc gggtttatcg aaggcgtgac ccttaaacgc ggtgagccgc
    12181 tcgattcgcc cgaacacgcc caacgcctga agatggcgga ttttccccaa ggcgcggaat
    12241 tggtgcctaa tccgtttaat aatatcgccg gattttccat tcgcgagcat tatttcttcc
    12301 ctggttttcc tgtgatggcg cacccgatgg cggaatgggt tttggatact tattacgccg
    12361 gccgcttcaa ccaaacccaa cgcggcagcc gcagcgtgta tgtgttcgag cagcccgaat
    12421 cgcgcattac gccgattatg gagcatatcg agcaaactta tctcggggtg cgttcgtaca
    12481 gcctgcccag cgtcggttgg acgcattcag acggcacgca ggtcaaaccg cacattgagt
    12541 tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg gcggaagtgt
    12601 tgcacagctt ggaaggattg ggagcagagc tgaaaaatcg ggtaaactga aaaacgtttg
    12661 cccaaatcgg acgatattta aggaaaacca tgcaataccg aatcagaaga gaaaacgaag
    12721 cacccgaagc aaaaaatgcg ggggaaacct tatgggagcg cgatatgatg cgcgaagtgc
    12781 tgctgtccgc ctatcgggac aggcgcaggg agcggatgtg gaaaaacatc tggcgcgccg
    12841 tcagcaccct gattctggtt gccctgattg caggcatttt ccgaaaagac gaagccgcat
    12901 tgcagttggc gggcaatacg ccgcataccg cagtcgtcaa cctgtacggc gaaatcggca
    12961 acggtgtaga agaccaggtc aaaaaactca aagacggtat ggaggccgcc tacaaaaacc
    13021 cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc gtcgtctcca
    13081 acaccgcttt tgaagaaata cgccgtctga aggcgcagca ccccggcatt cccgtttatc
    13141 tcgtggcgga agatatgtgc gcgtccggct gctactacat cgcggcggcg gcggacaaaa
    13201 tctatgccga cccgtccagc atcgtcggca gcatcggcgt tatcggcagc agtttcgacg
    13261 cgaccggcct gatggaaaaa atcggcgtga aacgcagggt taaaattgcg ggcagcaaca
    13321 aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcgaaaatc tgggaagaaa
    13381 tgctgaccgg catacacggc gaattcatca aagccgtcaa aaccggacgg ggcggccgcc
    13441 tcaaattccg acaatatccc gacgtgttca gcggtcgggt gtacacgggt gcggatgcgc
    13501 tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga gacgtggtca
    13561 aagcccccga tgtggtggat tacactccga aggacgattt cggcagaatc ttgggcagac
    13621 gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt
    13681 gtttacagta tttctaaggg aataccaaaa taaaaatata cacaaaaaca ctcacctccc
    13741 cagtatgctg ccactaagtc agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag
    13801 gcaggttgtc cccgggaggt tttgtgcatc ctgataccga tcggttgttg ttgctcaaag
    13861 gacagaaggc cgctgataaa cgagattacc tgtttgtcgc tattgacgat ttttatactc
    13921 tgccattttg ccagacaaaa ccgcagacag tgctgccaag tttctgaccg aacatctggc
    13981 cgacccctgc ttgtacctga ttgagtacgt ttactctgac aatgataggt aatataaaga
    14041 gccgtccaac atgctttcgg tgcagtttgt tatgataatg ggattggttg gaggcttgcc
    14101 cgatttgctt gtccgcagac caacggtaag gcggagcggg ttatccgtac cttgatggag
    14161 atgtggcatg aggaacagtc gtttgacagt ccggaacatc ggcaaaagga gttgtgctgc
    14221 tttattaatt taggggctgc cctagatcac tagggaaatt caaattaagt tagaattatc
    14281 cctatgagag aaagtcgtct aagccggtat aaacaaaata aactcatttt gttatcgtgg
    14341 ctatgatgta ttggatgcgg gcgaatttag ccatttccgt atcaatcaca gcacacattt
    14401 tgccgaacga caaaaccata ttaatggaa
//
LOCUS       DAWKOD010000039        13956 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000039 DAWKOD010000000
VERSION     DAWKOD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.039"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(301..1566)
                     /gene="ftsY"
                     /locus_tag="WJK73_000875"
     CDS             complement(301..1566)
                     /gene="ftsY"
                     /locus_tag="WJK73_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ7557379.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETAQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGESIDDLRPFDARAFVDALLD"
     gene            1712..3280
                     /gene="msrAB"
                     /locus_tag="WJK73_000876"
     CDS             1712..3280
                     /gene="msrAB"
                     /locus_tag="WJK73_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ7557380.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3382..3867)
                     /locus_tag="WJK73_000877"
     CDS             complement(3382..3867)
                     /locus_tag="WJK73_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ7557381.1"
                     /translation="MDALHTIARNLTKKCQTVSCAESCTGGMLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3893..4741)
                     /locus_tag="WJK73_000878"
     CDS             complement(3893..4741)
                     /locus_tag="WJK73_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7557382.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4835..5836)
                     /locus_tag="WJK73_000879"
     CDS             complement(4835..5836)
                     /locus_tag="WJK73_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7557383.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5895..6626
                     /locus_tag="WJK73_000880"
     CDS             5895..6626
                     /locus_tag="WJK73_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ7557384.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            6602..6874
                     /locus_tag="WJK73_000881"
     CDS             6602..6874
                     /locus_tag="WJK73_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ7557385.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            6929..8299
                     /gene="glmU"
                     /locus_tag="WJK73_000882"
     CDS             6929..8299
                     /gene="glmU"
                     /locus_tag="WJK73_000882"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ7557386.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLEAWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRT
                     SYLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDSRGRLKHGQDVVIDVN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVNKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            8374..8703
                     /locus_tag="WJK73_000883"
     CDS             8374..8703
                     /locus_tag="WJK73_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ7557387.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8775..10040)
                     /gene="efeB"
                     /locus_tag="WJK73_000884"
     CDS             complement(8775..10040)
                     /gene="efeB"
                     /locus_tag="WJK73_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ7557388.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRMLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQLDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(10304..11470)
                     /locus_tag="WJK73_000885"
     CDS             complement(10304..11470)
                     /locus_tag="WJK73_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ7557389.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(11467..12306)
                     /locus_tag="WJK73_000886"
     CDS             complement(11467..12306)
                     /locus_tag="WJK73_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ7557390.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            12517..13842
                     /locus_tag="WJK73_000887"
     CDS             12517..13842
                     /locus_tag="WJK73_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ7557391.1"
                     /translation="MKKYLFRAALYGISAAILAACQSKSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGETAGKMKEPGYVWQLLPNGMKPEYRP"
BASE COUNT         3202 a         4167 c         3591 g         2996 t
ORIGIN      
        1 caaaatcaga aacctaaaat cccgtcattc ccacgaaagt gggaatctag gacgcggaat
       61 ctcaagaaac cgtttacccg ataagtttcc gtgccgatgg ttctagattc ccgcctgcgc
      121 gggaatgaca ggtcttttat aacctttgaa tattgctgtt atcccaaggt ctagattccc
      181 gcctgcgcgg gaatgacggg tcttttataa cctttgaata ttgctgttat cccaaggtct
      241 agattcccgc ctgcgcggga atgacggcat cggtctgctg ttttcggacg gcatttcggc
      301 tcaatccagc agtgcgtcca caaacgcgcg cgcgtcaaac gggcgcaggt cgtctatgct
      361 ttcgcccacg ccgatgtagc ggacggggac ggggcggtcg gaagcaagcg cggcgaggat
      421 gccgcctttt gccgtgccgt cgagtttggt cacgattaaa ccggtcagcc ctaatgcgtc
      481 gtcaaaggct ttgacttggt tgacggcgtt ttgcccgatg ttggcatcaa gcacgacgat
      541 gatttcgtgc ggcgcgtcgg gcatggcttt ttgcagcacg cgcttcactt ttttgatttc
      601 ttccatcaag tgaagctgcg tgggcaggcg gccggcggtg tcggccagca caatgtcgat
      661 gccgcgcgct ttggcggctt ggacggcatc gaagcacacg gcggcggaat cgcccgtggt
      721 ttgcgaaatc acggttacgt tgttgcgttc gccccaagct tgaagctgct cacgggcggc
      781 ggcgcggaaa gtatcgccgg cggcaagcag cacagatttg ccctgcgctt ggaaatattt
      841 ggcgagtttg ccgatggatg tggttttgcc cgcgccgttg atgccagcaa gcatgatgac
      901 aaacggctct ttggtttcgg gcaaaaccag cggtttctct agaggcttaa tcaggtcgta
      961 caaggcttct ttcaacgcgc cgcgcaattc gttgccgtct ttcagccctt tgaggctgac
     1021 gcggtcgcgc acgtctttca tcaggtattc ggtggcttcc atgcccatat cgctggtaat
     1081 cagcacggtt tccagctctt cgtacaaatc ctcgtcgatt tgtccgccgc cgaacacgcc
     1141 cgccagcgat ttcgccattt tgtcgcgcga tttggtcaaa ccttgtttca aacgcgccgc
     1201 ccaaccgagc ttgtgttctt cagttgttgc aacggcttct tgaacttgcc cgacagcctc
     1261 gccgacggtt tcggcaatgg cttcttttgc cgcttcgact tgttccgctg ctttttccgc
     1321 cgcttcctct gcttcagaca gcatctcggc aacggcttcc tttacttgtt caaccgcacc
     1381 gctgacggtt tcaacagccg attcgacctg ccctttgacg ctttctgcta aagattcagc
     1441 atcttcttta atattttcaa ttacttgagc ggtttcggac tctgcttttg ctgcggtttc
     1501 ctgaatttga gcctcctcga gagccggcgt ttcctgtttt ttcttgcgac ggaagaagct
     1561 gaacattgaa ttttcctttt aattttagaa acttgaaata gggtgtattg tagcgtattt
     1621 tgcgcggcaa ggttgtctga aaatccgggc tgtaaggttt cggcatctca aacgtctaat
     1681 catgcaaacc gtccacacag gaaacattaa aatgaaacac cgtactttct tttccctttg
     1741 cgccaagttc ggctgcctgc ttgcgctggg tgcttgttcg cccaaaatcg tcgatgccgg
     1801 agccgcgacc gtgccgcaca ctttatccac tttgaaaacc gcagacaacc gccccgccag
     1861 tgtttacttg aaaaaagaca aaccgacgct gattaaattt tgggcaagct ggtgtccttt
     1921 gtgtctgtcc gaattgggac agaccgaaaa atgggcgcaa gatgcaaaat tcagctccgc
     1981 caacctgatt accgtcgcgt ctccgggctt tttgcacgag aaaaaagacg gcgacttcca
     2041 aaaatggtat gccggtttga attatcccaa gctgcccgtc gtaaccgaca acggcggcac
     2101 gatcgcccaa agcctgaata tcagcgttta cccttcgtgg gcgttaatcg gtaaagacgg
     2161 cgacgtgcag cgcatcgtca aaggcagcat caacgaagcg caggcgttgg cgttaatccg
     2221 cgacccgaat gccgatttgg gcagcttgaa acattcgttc tacaaacccg acactcagaa
     2281 aaaggattca aaaatcatga acacgcgcac catttacctc gccggcggct gcttctgggg
     2341 cttggaagcc tatttccaac gcatcgacgg cgtggttgac gcggtatccg gctacgccaa
     2401 cggcaacacg aaaaatccga gctatgaaga cgtgtcctac cgccatacgg gccacgccga
     2461 aaccgtcaaa gtgacctacg atgccgacaa actcagccta gacgacatcc tgcaatattt
     2521 cttccgcgtc gttgatccga ccagcctcaa caaacagggc aacgacaccg gtacgcaata
     2581 ccgcagcggc gtgtactaca ccgaccccgc cgaaaaagcc gtcatcgccg ccgccctcaa
     2641 acgcgagcag caaaaatacc aactgcccct cgttgttgaa aacgagccgc tgaaaaactt
     2701 ctacgatgcc gaggaatacc atcaggacta cctgattaaa aaccccaacg gctactgcca
     2761 catcgatatc cgcaaagccg acgaaccgct gccgggcaaa accaagaccg ccccgcaagg
     2821 caaaggcttc gatgcggcaa cgtataaaaa accgagtgac gccgaactca aacgcaccct
     2881 gaccgaagag caataccaag ttacccaaaa cagcgcgacc gaatatgcct tcagccacga
     2941 atacgaccat ttgttcaaac ccggcattta tgtggacgtt gtcagcggcg aacctttgtt
     3001 cagctccgcc gacaaatatg attccggctg cggctggccg agcttcacgc gcccgattga
     3061 tgcaaaatcc gttaccgaac acgatgattt cagctacaac atgcgccgca ccgaagtgcg
     3121 cagccacgcc gccgattcgc atttgggaca cgtcttcccc gacggcccgc gcgacaaagg
     3181 cggactgcgc tactgcatca acggcgcgag cttgaaattc atcccgctgg aacaaatgga
     3241 cgcggcaggc tacggcgcgt tgaagagtaa agtgaaataa gccgcaccgc cgcctaaccc
     3301 gacaaaatac cgtctgaaac ctgcaacgtt tcagacggta ttttttatcc ggcggggatt
     3361 ctgtccagac ggagattttg tttagacagc atcgccgccg ttttcaatca gccccgccaa
     3421 ccgttccaac gcgaaggcga ccgcctgcgc gcggacggat tcgcggttgc cgtcaaaacg
     3481 gcgcattgct tcgcaacttc cgcccggaaa ggcaaacccg aaccaaaccg tgccgacggg
     3541 tttgctttcg ctgccgccgc ccggaccggc gatgccggaa ataccgacgg cgtaatccgc
     3601 ctgcgccacg gctttcgcgc cgcgcgccat ctcatagacg gtttggcggc tgaccgcgcc
     3661 gtgttcgagc agggtttcgg gcaacacgcc caagcggtct tctttggctt tgttgctgta
     3721 tgttacaaaa ctttgatcga accattgcga actgcctgca acgcttgtga atacggcggc
     3781 aagcattccg cctgtacagg attcggcaca gcttacggtt tgacattttt tcgtcaggtt
     3841 tcgggcgatg gtgtgcagcg cgtccatttc ccactcccct ttcagacggc gtttaagaat
     3901 tgatgatgtg tatgtcgcgt tgcgggaacg ggatgttgat attgactttg cggaggtttt
     3961 cgaccacttg ttcgttcaag tcgcattgca gcgtccagcg gtctgcttcg tttgcccaag
     4021 cccataatgt gatttcgatg gcattgtcgc ccaaggcggt gatgtaggcg gcggcctgcc
     4081 gctcttcgtt ttgaacgctc aaggggtgtt cgacggcggc tttcaacacc gcctctttcg
     4141 ccactttcaa atcgcagttg taatcgacgc cgactatcac ttgggcgcgg cacagcggca
     4201 gtgtggaacg gttgacgatg ctgttgccca tcaccacgct gttgggcagc acgacttctt
     4261 cgttgtcggt cgtccgcaaa gaagtctgca ccattttaat ctctcggaca tatccttcaa
     4321 aaccgccgac gcggataaaa tcgccgactt tgaacgggcg gaacaggata atcagcgcgc
     4381 cggcggcaaa attggacagc tggtctttca aggacaacgc caccgccaaa cccgcgccgc
     4441 cgattaaggc ggttacggat gttgtggaaa cgcccaatct gcccaatgcg gcaataatca
     4501 ccaaaatcaa taagccgata ttggcaacat tacacaaaaa actaatcagc gtggcatcga
     4561 cctgcgcgcg cgtcatcgcc gccctcatca cggcgacaat gcgtttcgcc gcccattttc
     4621 cgaccaaaaa aataagcagc gcggcggcaa ggttcagccc gaacgcccac gccttttcag
     4681 ccagatgctc ccaaccggaa acactgatca ggtgtaaaaa atcaaattgt ttgaagtcca
     4741 ttgttttcct cttaatcgaa catccgcccc gtgcggcgta atcggcacag gtgtaaaaat
     4801 gccgtatgaa gccctgcggg gcggtatgtt tgttttattt caaaaccgtc ctgatccaac
     4861 gggaaaccca tccgcgctgg ttcgcatcaa tatcgcgctg cgcgggggcg gtggtgtgcg
     4921 tcggggcttg ggcgaagcgg aacacgtcgg gcaggcgcgt gtttttgacg gcggggtaaa
     4981 cccacatttc ggacggaacc gcctgctgca cttcccgact ttgcagccat tgcaccagtt
     5041 ttgccgccag cttcggctgt ttcgcgccct tcaagaccgc cgcgccttcg acctggcgga
     5101 atacgccgcc ttttaaaaac aggttgcccg tcggcggctc gctgtatttg cctttggaaa
     5161 aatacacttc cgccgccggg ctggcggcat aaccgaccac cagcggatac gcgccgccgt
     5221 tgtgcgaaaa gtcggtgtaa tacgcctcgc tccagccttt ggcgaccttc acgccgttct
     5281 gccgcatctg tgcccaccat ttgaacgcgc cttcttcgcc cagaccgctg atgttcgcca
     5341 tcaggaagcc cagccccggg gacgacgtgg cgggagacgg cacgaccaat aggtttttat
     5401 attcggggcg ggtcaaatcc tgcagggttt gcggcagagg aagctttttg ccttcaaacc
     5461 attttttgtc gtaattgatg gacacatagc cgtaatcgac cgccaaagcc gaaggcagcc
     5521 cgaccgcgac gggggcggat tcgggttgcg ccgccgccaa aatgcccatt tcccgcgcct
     5581 tgccgatatt ggcgttgtcc aaaccgtaca ccgcgtcggc aatcgggttg gcgcggctca
     5641 aaatcagttt gttgagcatt tcgttcgcgc cgcccgcctg aataatcgac accttcgcat
     5701 cgtttgcccg ctcgaagcgc gcaatcagcc ctttgggcag gctgaacgac ttatgcaccg
     5761 ccagcctgac ttccgtctgc gcctgcaggt atgccgaaac cgccagcagc ggcagcagcc
     5821 aaattttccg tttcattccg agtcctcctc tattcgctgt aaaataacat tctaacaaat
     5881 tttcacggtt caacatgcaa gaaaacccga ccgtgtggct gttcgacctc gacaacacgc
     5941 tgcacaatgc cgatgccggc ctcttcacac tcatcaaccg cgctatgaca cgctatatgg
     6001 cacgccgcct caaactctcc gaatctgccg cgtccgacct gcgtcaagac tattggcacc
     6061 gctacggcgc aacgctcgcc ggactgcaaa tccaccatcc cgaaatcgac atcgccgaat
     6121 ttttgcgcga aagccatccg atcgatgcaa tcctgaccag gctgcacggc atgcctgaaa
     6181 cacaaaacac cctgagccgc ctaaaagggc gcaaggcagt tttttccaac ggcccgtcgt
     6241 tttacgtccg cgccgttgtc ggcgcactcg gtttggaaaa ccgtttcgac gcactgttcg
     6301 gcacggatga tttcggactg ctgtacaaac ccaatccgca agcgtatctc aatgtctgcc
     6361 gccttttgga cgtaccgccc gaatgctgca ttatggtgga cgacagcgcg gacaacctgc
     6421 atcaggcaaa agcattgggc atgaaaaccg tccggttcgg tgcaaaagtg cacgacctgc
     6481 cctttatcga tgcttccgta agcgatatgg cgcaactggc tcggtatgca gaaactttgt
     6541 cagaacaccg ccaaaatcat tacaataccc gcacaccccc ccgaaaatac gaaagaatgt
     6601 tatgcgtaaa accttcctta tcctgatgac tgccgccgcc cttttgtcgg gctgcgcgtg
     6661 ggaaacttat caagacggca acggcaagac cgccgtccgt caaaaatatc ccgccggcac
     6721 gcccgtttat taccaagacg gcagctactc gaaaaatatg aactacaacc aataccgtcc
     6781 cgaacgccat gccgtgttac ccaaccaaac cggcaacaac gccgacgaag agcatcgcca
     6841 acactggcaa aagcccaaat ttcaaaaccg ataaacccac cctatgccgt ctgaagccct
     6901 ttcagacggc attgcacagg aaaacgctat gccgcaaaac actttaaaca tcgtcatcct
     6961 cgccgccggc aaaggcacgc gcatgtattc caaaatgccc aaggttttgc acgaaatcgg
     7021 cggcgaaaca atgctcgggc gcgttatcga caccgccgcc gcgctgaatc cgcaaaacat
     7081 ctgcgtcgtc atcgggcacg gcaaagacca agttttaaac gctgtcaaac gcgatgtcgt
     7141 ttgggttgaa caaaccgaac agctcggtac cggccacgcc gtcaaaaccg ccctgcccca
     7201 ccttgccgcc gaaggccgca cgctggtgtt gtacggcgac gttcccctga ttgacgttga
     7261 aaccctcgaa accctgctcg aagccgcagg caacgaagtc gggctgttga ccgacgtgcc
     7321 cgccgacccg acaggcttgg ggcgcatcat ccgcgacggc aacggcagcg taaccgccat
     7381 tgtcgaagaa aaagatgccg atgccgccca aaaggccgtg aaagaaatca ataccggcat
     7441 tttggttctg cctaacgcca aactcgaagc ttggttgaac agcctttcca gcaacaatgc
     7501 ccaaggcgaa tactatctga ccgacctcat cgccaaagcc gttgccgacg gcatcaaagt
     7561 ccatcccgtc cgagtgcgca cctcctacct cgccgccggc gtgaacaaca aacgccaact
     7621 cgccgaactc gaacgcatct tccaaactga acaggcgcaa gaattgctca aagcaggcgt
     7681 taccctgcgc gatccggcgc gtttcgattc acgaggtcgt ctgaaacacg gacaagatgt
     7741 cgtaattgat gtgaactgta tctttgaagg cgaaatcgag ctcggcgaca acgtcgaaat
     7801 cggcgcaaac tgcgtcatca aaaacgccaa aatcggcgca aacagcaaaa tcgccccctt
     7861 ctcccacctc gaaagctgcg aagtcggcga aaacaaccga atcggcccat acgcccgcct
     7921 gcgtccgcaa gccaaactgg ctgacgatgt ccacgtcggc aacttcgtcg aaatcaaaaa
     7981 cgccgccatc ggcaaaggca ccaaagccaa ccacctcacc tacattggtg atgccgaagt
     8041 cggcagcaaa accaacttcg gcgcaggtac gattattgcc aactacgacg gcgtgaacaa
     8101 acacaaaacc gtcatcggcg acgaagtgcg catcggttca aactgcgtac tggttgcccc
     8161 tgtaacactg ggcaacaaag taaccacagg cgcaggcagc acgattaccc gcaatatcga
     8221 agacaacaaa ctcgccctcg cccgcgcccg ccaaaccgtc atcgaaggct gggtgcgtcc
     8281 cgaaaaagac aagcaataaa gccatgccgt ctgaagccgg tttcaggttt cagacggtac
     8341 cccaaaacaa acatccgata aggactgcaa accatgtcat tacccccatg cccacaatgc
     8401 gcctccgaat acacttatga agacggcgga caatacatct gccccgaatg cgcccacgaa
     8461 tggaatgaaa ccgaatccgc cgccgacctt gcggctcaag tgcgcgatgc caacggcgcg
     8521 gcactgcaaa acggcgatac cgtcatcctc atcaaagacc tcaaggtaaa aggcagctcg
     8581 atggtgatca aacaaggtac aaaagtcaaa ggcatacgcc tgcaggaagg cgatcacaac
     8641 atcggctgca aaatcgacgg cagcgcgatg aatttaaaat ccgaattcgt caaaaaagcc
     8701 tgaccgccca aaaacaagaa acgccgtccg aacccgtttg gcaaggttcg gacggcgttt
     8761 tttatatggc ggatttatac gcccagcagc ccttgcccca aaaagccgcc tttttccacg
     8821 ccgggcaaga cgaagaaata gccgccgccg aaggggctga tgtattcttc cagcggttcg
     8881 ccgttgagga ggttttgcac gaagatgaat ccgtcggcaa ggtttgcctg atagcagacg
     8941 aacaccagtc cgacatcaag ctgtccgctt gaggcaagtc cgcgcgaata gctgtaggcg
     9001 cggcggaaga ggcggtgttt tttgaggaat tcgggatcgc gcggattcgc caggcgtata
     9061 tggctgtctt tgggcgtggt attcccctcg gggtctttgg caaaatccag ttggtcggct
     9121 tcttttttgc cgtccatcgg cgcaccgctg tatttgcgcc gcccgaaaat gtcggtttgc
     9181 tcttgaagcg gcgtcctgtc ccaaaactcg acaaagtggc ggataaggcg gactgcctga
     9241 tagctgccgt ttttcgccca ctccggttcg tcgaggctgt tggcggccac acccgtccac
     9301 aaaacctcgt cggcggtttt ggggtcggaa actttggggt tgcccgtgcc gtcgcggaag
     9361 cccaacaggt tgcgcgccgc catcgcgccg ggttcggatt taggctgcca cccgtcgata
     9421 ctccagcgga taacggcggt ttggacggtg tgtttgatga tgtcgcgcag ggcggcttgg
     9481 caggtttcgg gggtgaaggc acagatttgc aggctcaaat cgccgtcgca ccagcttttt
     9541 tgcagcttat cgttggagaa gtcgcgcatt tcctgcaaat gaatcggttt tttgtctttg
     9601 agtccgaacc ggccgtcaaa caggctgctg cccaccccca cggtaacggt caacccgtcg
     9661 gggttgaagg ctttgcccaa aatgccgctg ccggctggcg gaagtttgtc gtcgccgtct
     9721 tggtattcgc cgccttgggt gagaaactcg atgcgggcgg tcagcatgcg gaacaggttt
     9781 tccagctgct tggcactttg tgcggttacg tcgaaggcgc acataatcga aaacgcctgc
     9841 tgcggcgtaa cgatgcctgc ctgatgttcg ccgtagcagg gataggcttg gggcgagtgt
     9901 tggctttcgg cggtgcgttc ggcggtttcg ccccgttttt tgccgccgag ataacctccg
     9961 attgcgccga ctgctccggc tgcgatcgcg gttttaaaaa gagtgcgcct ggtcggttgt
    10021 gcgggttggt ttttgctcat ggtgtttcct tcaatatccg tgcagcagga tgggtatccc
    10081 tgcacgccgt ttttccatta accgatagcg gcaggttgaa atcccgccct gcaatatatg
    10141 gcggatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtataaat
    10201 agtacggcaa ggcaaggcga ggcaatgccg tactggtttt tgttaatcca ctataaaact
    10261 tcagacggta tctgccgcca aataccgtct gaacgcttgc ggcttatttc aagccgagta
    10321 tgccgcgaag ttgggcaagg tcttcggcaa gcgcgttaat agaggcctgt aacgctttgc
    10381 ggtcggcttc gcccagcttg tcgtaggttt caaaaccgtc tttagtccgg tatttcgcca
    10441 gaatttcgtt gacctgtttg aagttggtat cggttttttc caacaaggct ttgtttttgg
    10501 cctcgatcag cggacggaac aaatcgacga tttttttaga tccgtccaca ttggcttgga
    10561 agtcgctcaa atcggtgtgg ctgtaccggt cttcttcgcc gctgatttta ctgcccgcca
    10621 cttcttcaat cagttcggac gcgccgccga ccaccttgcc cggaggaaac gccaatgcgt
    10681 cgatttcttt ttgcagggct tcgacatcgg tcatcagttt cgctgcaatt tccttcacgc
    10741 cggacacgtc tttttccacc caaagggcgt attcgatacg gtggaagccg gtaaatccgg
    10801 catctttcgc gccgtctttg aagtcgtctt cacgcgcatc gatgacgggg tcgagttcgc
    10861 tgaaaagctc ggcaatcggt tcgatgcgtt cgtaatggac gcgggtgtcg gcaaacaggg
    10921 atttcgcctt ttcaatgtcg cctgctttga cggcttcggt aaaggttttg gttttcgcca
    10981 ccagctcttt aacctcgcct tgaacgtagg ctttatagtc ggcgagcggt tgggacagtt
    11041 tttccaaatc cgcttcgttg gcggtgtctt taaagccgct gtcggttacc accagcttgc
    11101 cgcgcggatt ggtcaaaaga ccgcaagtca tttcgtattc gcccggcaac agggtgacgg
    11161 tcattttatc ggaaagtccg ggggcgatgt tttcgcgctc gtccaccacc atcacgcctt
    11221 tcaggatttc ccattcgagc ttgcggccgc tgttgttttt aatattgaac acaacctgtc
    11281 cgctcggcac ggtcagttcc atcggttcgc aggcattgtc gttgacggcg atactgaccg
    11341 aaccgccctc gttggcggtt tgcgcctcac cggacgcggc cggcgcagct ttctccgcct
    11401 ccggcggctg gcacgcggtc aaacctaagg caagcatcac ggacaatgcg gtcaaattga
    11461 attttctcat ttcagctcct ctttacgggt taaagtttca gacggcctgc tgccgcacaa
    11521 aaaccaagtc atgacgggaa taaggtacag cagccaaacc aaggcctcgc cctgcgtcgg
    11581 atggtcggta tagccgaaaa atccgccgag cagcacacct aacggactgt cttcgtgcaa
    11641 atattttgat gagtcgaaca caatgtcctg aagcgcgttc caaatgcctg cttcgtgcag
    11701 cgcgcgcagc gagccggcaa gcaggccggc ggcaacgaca atcagaaacg cccccgtcca
    11761 acggaaaaac ttcgccagat tcaggcgcat cccaccctga taaatcaacg cgccaatcac
    11821 ggcggcagcc aaaacccccg ctaccgcacc ggccggcatc tgccaagtcg ggctctgttt
    11881 gaatacggca agcagaaaaa aaacgctctc caaaccttcg cgcgccacgg caagaaacgc
    11941 cataccgacc aaggcccatc cttgtccgct gccccggttc aaagccgcct gcacagaatc
    12001 ctgaagctgc tgcttcatcg aacgcgccgc ctttttcatc cataaaacca tataagtcag
    12061 catcgcgacg gcaaccaggc cgataatgcc gacgacgaac tcctgctgct tttggggaat
    12121 ctcgcccgtt gccgaatgga tgccgtaccc cagccccaaa cacatcaaag aagcaaggac
    12181 gaccccgaac cagaccttag gcatcagttt ggaatgtccg gactgtttca gaaaaccggc
    12241 aacgatgccg acaatgagcg cggcttcgat accctcgcgc aacataatta aaaaagcgac
    12301 cagcataaac gcgaacgaac aaggatgatg aataatatat tatcggaata ttttcattgc
    12361 ttgtaaatac aaatgcaagt tatttttatc tgcagtaccg cgcggcggaa agttccgcag
    12421 gctgcagctg cgccctgtgt taaaatcccc tctccacggc tgccgcaacg ccgcccgaaa
    12481 ccatctttct tattactgcc ggcaacattg tccattatga aaaaatacct gttccgcgcc
    12541 gccctgtacg gcatctccgc cgccatcctc gccgcctgcc aaagcaagag catccaaacc
    12601 tttccgcaac ccgacacatc cgtcatcaac ggcccggacc ggccggccgg catccccgac
    12661 cccgccggaa cgacggttgc tggcggcggg gccgtttata ccgttgtgcc gcacctgtcc
    12721 ctgccccact gggcggcgca ggatttcgcc aaaagcctgc aatccttccg cctcggctgc
    12781 gccaatttga aaaaccgcca aggctggcag gatgtgtgcg cccaagcctt tcaaaccccc
    12841 gtccattcct ttcaggcaaa acagtttttt gaacgctatt tcacgccgtg gcaggttgca
    12901 ggcaacggaa gccttgccgg tacggttacc ggctattacg aaccggtgct gaagggcgac
    12961 gacaggcgga cggcacaagc ccgcttcccg atttacggta ttcccgacga ttttatctcc
    13021 gtccccctgc ctgccggttt gcggagcgga aaagcccttg tccgcatcag gcagacgggg
    13081 aaaaacagcg gcacaatcga caatgccggc ggcacgcaca ccgccgacct ctcccgattc
    13141 cccatcaccg cgcgcacaac ggcaatcaaa ggcaggtttg aaggaagccg cttcctcccc
    13201 taccacacgc gcaaccaaat caacggcggc gcgcttgacg gcaaagcccc gatactcggt
    13261 tacgccgaag accctgtcga actttttttt atgcacatcc aaggctcggg ccgtctgaaa
    13321 accccgtccg gcaaatacat ccgcatcggc tatgccgaca aaaacgaaca tccctacgtt
    13381 tccatcggac gctatatggc ggataagggc tacctcaaac tcggacaaac ctccatgcag
    13441 ggcattaagt cttatatgcg gcaaaatccg caacgcctcg ccgaagtttt gggtcaaaac
    13501 cccagctata tctttttccg agagcttacc ggaagcagca atgacggccc tgtcggcgca
    13561 ctgggcacgc cgctgatggg cgagtacgct ggcgcagtcg accggcacta cattaccttg
    13621 ggtgcgccct tatttgtcgc caccgcccat ccggttaccc gcaaagcact caaccgcctg
    13681 attatggcgc aggataccgg cagcgcgatt aaaggcgcgg tgcgcgtgga ttatttctgg
    13741 ggttacggcg acgaagcagg ggaaacggca ggtaaaatga aagaaccagg ttacgtttgg
    13801 cagctcctgc ccaacggtat gaagcctgaa taccggccgt aaaccggcaa aacaatgccg
    13861 tctgaagccc atcacggcca atcgggcgca ttaaggcgaa gtttgcgata ccgccggtac
    13921 aagccggctg gacaaaacgc ctttcttatt tgacgg
//
LOCUS       DAWKOD010000040        13840 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000040 DAWKOD010000000
VERSION     DAWKOD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.040"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(228..551)
                     /locus_tag="WJK73_000888"
     CDS             complement(228..551)
                     /locus_tag="WJK73_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ7557392.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WJK73_000889"
     CDS             complement(632..1288)
                     /locus_tag="WJK73_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ7557393.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1390..1893)
                     /locus_tag="WJK73_000890"
     CDS             complement(1390..1893)
                     /locus_tag="WJK73_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ7557394.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2077..2850
                     /locus_tag="WJK73_000891"
     CDS             2077..2850
                     /locus_tag="WJK73_000891"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ7557395.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2857..3207
                     /gene="secG"
                     /locus_tag="WJK73_000892"
     CDS             2857..3207
                     /gene="secG"
                     /locus_tag="WJK73_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ7557396.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3221..3306
                     /locus_tag="WJK73_000893"
     tRNA            3221..3306
                     /locus_tag="WJK73_000893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Leu,seq:gag)
     gene            4107..4475
                     /locus_tag="WJK73_000894"
     CDS             4107..4475
                     /locus_tag="WJK73_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7557397.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4472..4783
                     /locus_tag="WJK73_000895"
     CDS             4472..4783
                     /locus_tag="WJK73_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ7557398.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5120..6078)
                     /locus_tag="WJK73_000896"
                     /pseudo
     CDS             complement(5120..6078)
                     /locus_tag="WJK73_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(6101..6214)
                     /locus_tag="WJK73_000897"
     CDS             complement(6101..6214)
                     /locus_tag="WJK73_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ7557399.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
     gene            complement(6344..7135)
                     /locus_tag="WJK73_000898"
     CDS             complement(6344..7135)
                     /locus_tag="WJK73_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7557400.1"
                     /translation="MNLFFDTELGRQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEKKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            complement(7367..10099)
                     /locus_tag="WJK73_000899"
     CDS             complement(7367..10099)
                     /locus_tag="WJK73_000899"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ7557401.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADTSKADLEASALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            10369..10884
                     /locus_tag="WJK73_000900"
     CDS             10369..10884
                     /locus_tag="WJK73_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7557402.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10891..11334
                     /locus_tag="WJK73_000901"
     CDS             10891..11334
                     /locus_tag="WJK73_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ7557403.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11503..13560
                     /gene="ppk1"
                     /locus_tag="WJK73_000902"
     CDS             11503..13560
                     /gene="ppk1"
                     /locus_tag="WJK73_000902"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ7557404.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
BASE COUNT         3442 a         3905 c         3392 g         3101 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcagac tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa accaagcaaa atgccgtctg aagccccaaa
      601 cccgctttca gacggcatcg caatccgaac atcaaaactg aaaaccacca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgcccctgcg cgtaatcaaa agcgcgcgtt gaacaggctt gcgtcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacttcagg cacaaggttt accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcaacccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 ccgcatcgat atcgtccgaa attaccctgc ccgccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctccagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaagtcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg ttccaacaat
     1321 agcaggcatt ataaaaccac actcccgccg cgccaacttc ggcacgcgcc gcccgcccct
     1381 ttccctcact caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ctcgcccaaa tacaaagcat ctgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgcgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gtccacatcc ccgacgacca ccgtcccgcc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgtctcg tccgccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacaa cctgcccgaa acaacattcc aaaccgcccc cgcagccgcc
     1981 cgccccaagt tgcgaacccg ccgcatatcc actaaacttc acattgcaac gcgccacacg
     2041 cggcagacag aaaaaacacg acacggagca aaaaagatgt atcaccaaat cggaatgtgg
     2101 gatcaaaaat gggtcatcgg caactggaaa atgaacggcc ggctccagaa caacaattcg
     2161 ctcatgcacc gcttccgcat cctccccacc gccgaacgcg tcctcatcgg actcgccgcc
     2221 ccgaccgttt acctgctgca actgcacaac gccatgcaaa tcgtcttaaa caaccgcatc
     2281 ctcacctgcg cccaagacgt gagccgcttc cccgacaacg gcgcgtacac cggcgaagtg
     2341 tccgccgaaa tgctcgccga caccggcaca gacatcgtcc tcatcggaca ctccgaacgc
     2401 agcctttatt tcggcgaaaa aaacgaaatc caacgccgca aaatggaaaa cgtcctcaac
     2461 gtcggactca tcccgttatt gtgcgtcggc gaaagcctcg aagagcgcga agccggcaaa
     2521 gaacacgaag tcatcgccca tcagctttcc atcctgcaag ggctggatac caaaaacatc
     2581 gccgtcgcct acgaacccgt ctgggcgatc ggcaccggca aagtcgccac cgtcgaacag
     2641 attgccgata tgcacgcatt catctacaaa gaaatcttgt ctttgtgcgg aagcgatgtt
     2701 aaaatccgcg tcctttacgg cggaagtgtg aaagcggaca acgcggccga catcttcgca
     2761 gtaccttatg tggacggcgc actcgtcggc ggcgcgtcat tgtcgtacga ctcctttacc
     2821 gccatcatca gtgccgcaca aaatgcgtag aaaaatatgg aagccttcaa aaccctaatt
     2881 tggattgtta atataatttc cgctttggcc gtcatcgtgt tagtattgct ccaacacggc
     2941 aaaggcgcgg atgccggcgc gactttcgga tcgggaagcg gcagcgcgca aggcgtattc
     3001 ggctctgccg gcaacgctaa cttcctcagc cgctcgaccg ccgttgcagc aacatttttc
     3061 tttgcaacct gcatggctat ggtgtatatt cacacccaca cgacaaaaca cggtttggac
     3121 ttcagcaacg tacaacaaac tcagcaagca cccaaacccg taagcaatac cgaaccttct
     3181 gcccctgttc ctcagcagca gaaataacag tttttcaaat gccgacatgg tgaaattggt
     3241 agacacgcta tcttgagggg gtagtggccg taggctgtgc gagttcaaat ctcgctgtcg
     3301 gcaccaacac acaaaacgct tagaaattct aagcgttttt ttcgtttaat cctgcctgat
     3361 gccatcaacc aatcccaatc aatacaaggt tttcaggctg attgttatcc tgccgccccc
     3421 ccttcctgac cgtacaatcc cgtccaatcc gccctaattg aactaacctg attttttacg
     3481 gcatccgaaa ataccgcgaa aaatacgtga aaataaagct gaacgaccac caaatcagga
     3541 atgccaagcg ggaaatagct tgcggggaat actgacgcac cgtagggaat gagggggaaa
     3601 ccgtccaaga tgcagagcgg ttttttttgg gtttttggaa aaaacctata ctatagtgga
     3661 ttaaatttaa actggtacgg cgttgcctcg ccttgccgta ccggtttaaa tttaattcac
     3721 tataaaataa gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg gtaacgaatc
     3781 ggtaacaact tttggggttt tccggttttt caccgtcttg gcagtgggag cgtagcggaa
     3841 tgaaaagcca aaacgcacgg aaccgcgcct attttgagca ggaatggcgg ttaaaccact
     3901 tggttatata tgggggcata ggaagacagc gaaacgcgct aaaaacgcaa tttgagccat
     3961 taaaagcgat tgattaaaaa ataatcaggt tagccgccag tgtttcaggc ggcataaacg
     4021 gagaaattgc ggggcataaa aaaggcagct tgccgtgttg tctgtctctg gtataattcc
     4081 aagtatcact aatcaacggc tacacaatgc ggatattcaa aaaccaatgg atagtgaaat
     4141 ttgccaagaa gcacaaaatc aacgattccg agctgctgga agcggtagag cgggcggata
     4201 acgggctgat agacgcagat ttaggcggcg gtgtgattaa gcagcgcata gcaaggcaag
     4261 gacaaggcag aagcggcggc tatcgcagcc tgatattgtt caaacaggca gatagggcat
     4321 ttttcgttta cgcctttgcc aagaacgaca gggaaaacat ttcggataaa gaactggacg
     4381 tttaccgaaa agccgccgca tattatctga aatacacgcg ggcagagctg gcggctttga
     4441 aagaagacgg cattatcacg gagatagaat catgaaatac aaaagcgagg cattagccgc
     4501 cattcatgaa atgatggaag gggcttacaa catcggcgca atcgacaaaa agactatgcg
     4561 cggctttgac aagtcatgcc tgaccgaaat caaaccgttg agcggcggag acatcaaggc
     4621 aatcagggag aaggaggcac tatcgcaagc cgctttcgcc atctatctca acgtgggaaa
     4681 aaatcacgtt tcggcttggg agcggggcgt taaaaagccg agcggcgcgg cgttgaagct
     4741 gctgaccatc gtcaaaaaca agggtatcga agccattgcg tagccgactt ggcaaacggc
     4801 aaaatcagca agttcacaat agacgcgctg ctgaatatgc ctgccaaaac aggcaagacc
     4861 gccgaactga atatcagggc gtagccgcat aaatgccccg accgcatcaa accaagccga
     4921 aacggcggcg gtgcagacgg catagcccga cagcaaggca cggggatttt tttgcgcccg
     4981 ttgcaggggg attggattta agcggcgcgg acttgaaggc aaaacgggcg gggcgcagaa
     5041 ctgtttaaat gccgtctgaa tctcaaacgg attcagacgg cattttggaa caacggctca
     5101 aattctcgat ccccttccct taacgccgac gttttttatt aacgcgcccc ttatttctga
     5161 cactttgctc ataaaccggc ataacggtcg gcaacaaccg ttttagattt tctatacggg
     5221 cattgtttgt cggatgagta gaggtaatag cataaataaa gccgttttgg tcgttttcct
     5281 gattcatttt ttcccaaacc ctgacagcgg ccgccggatg atagcctgcc tgcgccatca
     5341 acatcattcc cccctcatcg gcttcttctt ccaagctgcg gctataaggc aaggtaatac
     5401 cgtacgttcc caaaatatcc atacccaatc cgaccaattc cggattagta tccggttttt
     5461 tgtctaatat aatctgcgtg cctatctgcg ccgccgtatt ggtcaagatt tgctgcccga
     5521 ccttattttt accgtgttca tgcagggcgt gcgtcatttc atgtcccata atggcggcaa
     5581 tttcgtcatc ggtcagcttg agtttgtcga ctatccccgt ataaaacgcc attttcccgc
     5641 cgggcatcgc ccacgcgttc agctcatcgt ttttgaaaac cgtcattttc cagtcaaacc
     5701 tatggccggt attatttgcc gcatcggcat aaggcaacat acgtcgaaat actgcctgca
     5761 ccctacgggc tgttctggat gtggtatcga cattgccggc agacttgttt aactcaaccg
     5821 ttttcatata atcttcggca gccgcagcgt tcattgtggc ggaatcatga ccgtaaacat
     5881 cagcaacgac cgcacaacct cccaatatcg aaatgactgc cgacaggcag actatccgtt
     5941 taaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaatttcata
     6001 ttaggcttac cccttaaaaa attaaattta aaaaatgccg tctgaatcta aaacggattt
     6061 cggacggcat cttaacattg tttgatgttt ttaaaaagat ttacaccacg atgctcgcca
     6121 gtctgcccgg tacggcgatg attttcttgg caggattacc ttctatgaat ttcactgcgc
     6181 cttcggaggc ggccggtttg tcggaatcac gcataaattg ccaataattc cccgactttt
     6241 ttacggctgc tgccttttgc ggcaatattg cgctgaactt caactgtttt cagaatagca
     6301 gaagaataaa tatcccttgt gaattcagta tcatgatttg aaattaaaac gccttggaag
     6361 tcgggggtaa tttgtcgatt tttttgtaaa gtccgcgacc aatgaattcg atcgtatttt
     6421 ggtcgcgcaa aatttgcaac tgttggcgga ttttgtctct gatatggttg ttttggggaa
     6481 attggatgga tagttttttt tcaaattcat acatttgtga caatgtgaat tcttcgggga
     6541 gttggtcgat acatttcata acagccaaaa gccagccttt gcgctccgca ttttggttgc
     6601 gtaaaaacag gttggattgc cattttttca gaacggtttc gggttcgata atgcgggaat
     6661 cgtctattaa gaatattttt ccgctttcag gcaaaggggc gagattgatg gaacacataa
     6721 tgtggttcgg ccggttttta atacctttat ttctgggaat aatcatatcc ggcgtgatga
     6781 aatgtttggg tacaagcacc aattgccgta tggagtaatc cgctttttta tatgcaagaa
     6841 agaaaaagtt ggggttggta tctgaccgga tgcgctccag catggtgcga tatgcaccgt
     6901 caggcacgct gttgcctatg gttttttgat ttttactctt taattcatat tgctcgtggc
     6961 aatttgggca aaagaggtct gcaacaggtt tgttattggc aaatctctgc atcggcttgc
     7021 ttccgcaaca ggggcagtag ccgttttttt ccagccaagc ctcgctcatt acacggattt
     7081 tgtgggttgc tttattttgt tgccttccca attcggtatc gaaaaataaa ttcatgtttt
     7141 ggattttgag attaagtttg agtggattaa caaaaatcag gacaaggcga cgaagccgca
     7201 gacagcaggg caaggcgggg caacgccgta ccggtttaaa gttaatccgc tatatttgag
     7261 gtctgtcatg cggacaactc catctgcctt aaaaaggccg tctgaaaccc tgtttccaag
     7321 tttcagacgg cctttcctaa ttacagcagt caatgccgaa tcggttttac accacgatgt
     7381 tcaccagtct gcccggtacg acgatgattt tcttggcagg cttgccttcc atgaatttca
     7441 ccgcgccttc ggtggcgagt gcggaggctt cgaggtcggc tttggaggtg tcggcggcga
     7501 cggtgatttt gccgcgcagt ttgccgttga cttgaaccat gacttcgatt tcggatttga
     7561 ccaaggcggc ttcgtcgact gtcggccagc ctgcttccca cagtttcgca ccgttcaatt
     7621 cgctccacag ggtttcgcag atgtgcggca cgatgggcca cagcaggcgt acggcggctt
     7681 ccaatacttc ttgggcgacg gcgcggcctt gttcgccacc ggtgtcggtt ttgtcgtatt
     7741 ggttgagcag ttccatcacg gcggcgatgg cggtgttgaa ctgctgacgg cggccgtagt
     7801 cgtcgctgac tttggcgata gtggaatgca gtttgtggcg caggtctttg agttctttag
     7861 acaaaccgtc ttggctgcct gcgaacgcgt tgactgcttc gccttgcttc aagtattcgt
     7921 aaacggtacg ccacaggcgg cgcaggaagc ggtgcgcgcc ttcgacgccg ctgtcgctcc
     7981 attcgaggga ctgttcgggc ggtgcggcga acatcatgaa caggcgggcg gtgtctgcgc
     8041 cgtaggcgtt aatcagttct tgcggatcga cgccgttgtt tttggacttg gacatttttt
     8101 ccgtgccgct gatgacgacg ggcagtccgt cgactttcag gacggcggaa acagggcggc
     8161 ctttgtcgtc aaaagtcagc tcgacatcgg cggggttgat ccaatctttg ccgcctttgt
     8221 cgttttcgcg gtagtaggtt tcgcaaacga ccatgccttg cgtcagcagg cgttcaaacg
     8281 gttcgtcaac attgaccaaa ccttcgtcgc gcatcagttt ggtgaagaaa cgcgcgtaca
     8341 agaggtgcaa aatcgcgtgt tcgatgccgc cgatgtattg gtcgaccgcg ccccagtatt
     8401 tcgcggcggc aggatcgacc atgccgtctg aaaattttgg cgacatgtag cggaagaagt
     8461 accagcttga ttccatgaag gtgtccatgg tgtcggtttc gcgttttgcc gcgccgccgc
     8521 agcatgggca ggtggtttcg taaaactcgg gcatttttgc cagcggcgaa cccatgccgt
     8581 cgggtacgac gttttcaggc aaaacgaccg gcaattggtc ggcagggacg ggtacgtcgc
     8641 cgcattgttc gcaatggacg atgggaatcg ggcagcccca gtagcgttgg cgcgaaatgc
     8701 cccagtcgcg caggcggtat tgggttttcg gttcgccagc tccgatagct tgtaatttag
     8761 cggcgacagc atcaaatgct gtctgataat tcatgccgtc cagttcacct gagttgatta
     8821 agaccatgaa gtcatctttg actccatacc atgcttgcca tttattaggc ataaacggca
     8881 aatctcttga atcggaacca ctaccatcac cataaccagc accacatccg gaaccgccac
     8941 caaaacccat agcttcttct tgctctggcg aaagtccgat tgttacgcgt tgtaagacct
     9001 gttttttcgg caggctgtat ttggcggcga actcgaaatc gcgttcgtcg tgcgccggaa
     9061 ccgccattac cgcgccgtcg ccgtagcccc acaatacata gttggcaatc cacacttcca
     9121 gcttgtcgcc gttgagcggg ttgacgacgt agcggccggt cggcacgcct tttttctcca
     9181 tcgtcgccat atcagcttcg gcaaccgaac cggctttgca ttcggcaata aatgcctgca
     9241 attcgggttt gtcggcggct gcggcggttg ccagcggatg ctcggcggca acggcaacat
     9301 aagtcgcgcc catcagcgtg tcgggacggg tggtataaac ttgcaggaat ttcgcgtaat
     9361 cgccttccaa gccttgtttg ctgtcgtctg aaacggcgaa gcgcacggtc ataccgcgag
     9421 atttaccgat ccagttgcgc tgcatggttt tgacttgttc cggccagtgt tccagcttgt
     9481 ccaagtcgtt gagcagttct tcggcgtaat ccgtgatttt gaagtaatac atcgggattt
     9541 cgcgtttttc gatcaatgcg ccggaacgcc agccgcgtcc gtcgataact tgctcgttgg
     9601 caaggacggt ttggtcgacc gggtcccagt ttaccgtgcc gtttttgcga tagacgatgc
     9661 ctttttcaaa cagcttggta aacagccatt gttcccagcg gtagtattcg ggtttgcagg
     9721 ttgcggtttc gcgcgcccag tcaatcgcaa aacccaggct tttgagctgg gttttcatgt
     9781 attcgatgtt atcgtacgtc caagcggcag gggcgacgtt gtttttcatc gccgcgtttt
     9841 ccgccggcat gccgaacgcg tcccaaccca taggctgcat gacgttgaag ccgtttaaaa
     9901 gtttgaagcg gctcaataca tcgccgatgg tgtagttgcg cacatgcccc atatgcagct
     9961 tgccgctggg ataggggaac atggagaggc aataatattt gggtttggaa gcgtcttcgg
    10021 agacgttgaa aatacgggcg tcgtcccatt ttttctgcgc cgcaggctcg atggcggcgg
    10081 gttgatattg ttcttgcata gtcattctgt tttcgcttaa aaacgttgga aaaataaagt
    10141 cggcatcaat tataacaggt tgccggaagc ggcgaatcgg cagattgccg gcaggatgcg
    10201 taaattcgca cgcgcattat tccgtatgcc gtacaaatac accgcgttta ttgatacgca
    10261 cgttttttat gctaatatta caaaccaaaa tcaaatgttt aaaactctcc tgatgcggct
    10321 cttccgaaca aaaggcagac gggcatcggg taaaagagga ttctgcatat gaaaatcaaa
    10381 caaatcgtca aaccgggctt ggcagtattg gcggcgggcg ttctgtctgc ctgcgcaacc
    10441 aaaagcaacg tcaaagccga cggcacgacc gacaatccgg ttttcccgaa accctattcc
    10501 gtaacgctcg acaacaagcg cggcacattc ccgacttatg acgaactgga tcagatgcgc
    10561 cccggcctga ccaaagacga catctacaaa atcctgggcc gcccgcatta cgacgaaggt
    10621 atgtacggcg tgcgcgaatg ggattacctg ttccacttcc ataccccggg cgtaggtatc
    10681 gaccctgaaa acacttccgg cgtagaagat gttactacct gccaatacaa agtgattttc
    10741 gataaagaca aatttgcccg cagcttctac tggaaccccg tcttcccgaa agatgccgcc
    10801 tgtccgccgc ccgcacccaa agccgagccg caagtcatca tccgcgaaat cgtgccggca
    10861 aaaccgaaac gtatccgcca ataatccgac atgccgttcc gcctgttttt agggatatta
    10921 tgcggcctgt caatggttgc ccccgtatat gcacaggggc aaccggatgc ggtcaacgct
    10981 tatattcaaa agaaaaaagt catcgtaaac acatccaaag cggaactctg ttttgccgat
    11041 gacaggcagt gccaccccgt cctcatcggt gttgccacgc ccaaggggac gttcgggctg
    11101 acgctgaaca gtaccgacaa gcctggatac ggcggcgaag tcatcggttt caagcaggag
    11161 ggtgattttc ttttcgccct gcaccgcgtt tggaatcaga taccgtcgga aaggcggaac
    11221 gaacgcatcg cctccccgtc cgtgtccgac aggattatga ccaacggctg catcaacgtc
    11281 agcgatgcgg tgtacgaaaa actgcgtcat tattttgtgt tggaagtgat ttgaaacaga
    11341 cggataccgc acgcgccggt atctgttttc acattgcccc gatgcctgaa acaggctgtc
    11401 cgccacgtca tgccgtctga aaccggcgca gatgccgcca agccttcaga cggcattgcc
    11461 tgcccgctcc gaccgaacaa caaccatctt tgggagaacc ttatgcccga acaaaaccgc
    11521 atcctctgcc gcgaactaag cctgctggca ttcaaccgcc gcgtgttggc acaggcggaa
    11581 gacaaaaacg tccccctttt ggaacgcctg cgcttcctgt gcatcgtgtc gtccaatctt
    11641 gacgagtttt tcgaagtccg catggcgtgg ctcaagcgtg aaaacaagct gcacccccgg
    11701 cgcaggctgg acaacggcaa aatgccgtct gaaaccatcg ccgacgttac cgaagcggcg
    11761 cgctccctga tacggcacca gtacgacctg ttcaacaacg tccttcagcc cgagttggcg
    11821 cgggaaagca tccattttta ccgccgccgc aactggacgg gcgcgcagaa aaaatggatc
    11881 gaagactatt tcgaccgcga actgctgccg atcctgaccc ccatcggact cgacccgtcc
    11941 caccccttcc cgcgaccgct gaacaaatcg ctcaacttcg ccgtcgaact cgacggcacg
    12001 gacgcattcg gcaggccttc ggggatggcg attgtgcagg caccgcgcat cctgccgcgc
    12061 gtcgttcccc tgccgtccga actgtgtggc ggcggacacg gcttcgtctt cctgtcgtcc
    12121 atcctccacg cccacgtcgg caaactcttc ccgggcatga acgtcaaagg ctgccaccag
    12181 ttccgcctga cgcgcgacag cgacttgacc gttgacgaag aagacctgca aaacctccgc
    12241 gccgccatcc aaaacgaact gcacgaccgc gaatacggcg acggcgtgcg gctcgaagtc
    12301 gccgacacct gtcccgccta catccgcgac tttctgctcg cgcaattcaa actgaccgcc
    12361 gccgaactct atcaggtcaa aggcccggtc aacctcgtgc gcctcaacgc cgtccccgac
    12421 ctagtcaacc gccccgattt gaaatttccc acacacacgc cgggcagact gaaagccttg
    12481 ggcaaaaccg cgtccatatt cgatttggtg cgccaatcgc ccatcctgct gcaccacccc
    12541 taccaatcgt tcgaccccgt tgtcgaaatg atgcgcgaag ctgccgccga ccccgacgtg
    12601 cttgccgtca aaatgacgat ttaccgcacc ggcacgcgtt ccgaactcgt ccgcgccctg
    12661 atgaaggcgg cactcgccgg caaacaagta accgtcgtcg tcgaactgat ggcgcgtttt
    12721 gacgaagcca acaacgtcaa ctgggcgaag cagctcgaag aggcgggcgc gcacgtcgtg
    12781 tacggcgtgt tcggctacaa agtccacgcc aaaatggcac tggtcatccg ccgcgaagac
    12841 ggcgtactca agcgttacgc ccacctcggc acgggtaact accaccaagg cacatcgcgc
    12901 atctacaccg acttcggcat cattaccgcc gacgaacaaa tcaccgccga tgtgaacaca
    12961 ttgtttatgg aaatcacagg cttgggcaaa cccgggcggc tgaacaaact ctaccaaagc
    13021 ccgtttaccc tgcacaaaat ggttatcgac cgcatcgcac gcgaaaccga acacgcaaaa
    13081 gccggcaaac cggcgcggat taccgccaag atgaattcgc tcatcgaacc gaccgtcatc
    13141 gaagccctgt atcgggcaag cgcggcaggc gtacaaatcg atttgattgt gcgcggtatg
    13201 tgcaccttgc gcccgggtgt aaaaggcttg tccgaaaaca tccgcgtccg ctccatcatc
    13261 ggcaggcagc tcgaacacgc gcgcgtgtat tgcttccaca acaacggcgc ggacgatacc
    13321 tttatctcca gcgcggattg gatggggcgc aacttcttcc gccgcatcga aaccgccgcg
    13381 ccgattaccg cgcccgaact caaaaaacgc gtcatccgcg aaggtttgga aatggcattg
    13441 gcagacaata cgcacgcgtg gctgatgcag cccgacggcg gctatatccg cgccgcgccc
    13501 gccgagggcg aatccgaagc cgacctgcaa aacgatttgt ggacactgct cggaggctga
    13561 cccgcaccgc cccaatcaaa aaccatgccg tctgaaacct ttccgtttca gacggcatag
    13621 ttttacagca atctaaacag ggcggaccgg agtcaaaaac acaccttcgc cattcctgca
    13681 caagcacttc ccctatacgc tcccaacccc aagccgccgc attccagacg gcattatagt
    13741 ggattaaatt ttaggggctg tactagatta gcagatatgt taccctggga atatgaagat
    13801 aacacactgc aaattaaaga aaaaagtaca gaaagaactg
//
LOCUS       DAWKOD010000041        13540 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000041 DAWKOD010000000
VERSION     DAWKOD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.041"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..1226)
                     /locus_tag="WJK73_000903"
     CDS             complement(27..1226)
                     /locus_tag="WJK73_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ7557405.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            complement(1259..3784)
                     /gene="ligA"
                     /locus_tag="WJK73_000904"
     CDS             complement(1259..3784)
                     /gene="ligA"
                     /locus_tag="WJK73_000904"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ7557406.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVLHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            complement(3932..5218)
                     /locus_tag="WJK73_000905"
     CDS             complement(3932..5218)
                     /locus_tag="WJK73_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ7557407.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFTEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5412..5984)
                     /gene="ampD"
                     /locus_tag="WJK73_000906"
     CDS             complement(5412..5984)
                     /gene="ampD"
                     /locus_tag="WJK73_000906"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ7557408.1"
                     /translation="MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6068..7063
                     /gene="mltG"
                     /locus_tag="WJK73_000907"
     CDS             6068..7063
                     /gene="mltG"
                     /locus_tag="WJK73_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ7557409.1"
                     /translation="MLRKLLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7123..7743
                     /locus_tag="WJK73_000908"
     CDS             7123..7743
                     /locus_tag="WJK73_000908"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ7557410.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            7965..9245
                     /locus_tag="WJK73_000909"
     CDS             7965..9245
                     /locus_tag="WJK73_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ7557411.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            9328..9599
                     /locus_tag="WJK73_000910"
                     /pseudo
     CDS             9328..9599
                     /locus_tag="WJK73_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9639..10673
                     /locus_tag="WJK73_000911"
     CDS             9639..10673
                     /locus_tag="WJK73_000911"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ7557412.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEVGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10879..11460
                     /locus_tag="WJK73_000912"
     CDS             10879..11460
                     /locus_tag="WJK73_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ7557413.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11529..11711
                     /locus_tag="WJK73_000913"
     CDS             11529..11711
                     /locus_tag="WJK73_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ7557414.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            11708..12469
                     /gene="kdsB"
                     /locus_tag="WJK73_000914"
     CDS             11708..12469
                     /gene="kdsB"
                     /locus_tag="WJK73_000914"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7557415.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            12492..12809
                     /locus_tag="WJK73_000915"
                     /pseudo
     CDS             12492..12809
                     /locus_tag="WJK73_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            12914..13111
                     /locus_tag="WJK73_000916"
     CDS             12914..13111
                     /locus_tag="WJK73_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557416.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13268..13360
                     /locus_tag="WJK73_000917"
     tRNA            13268..13360
                     /locus_tag="WJK73_000917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13302..13304,aa:Ser,seq:gct)
BASE COUNT         3019 a         3371 c         3952 g         3198 t
ORIGIN      
        1 acgccgtact ggtttttgtt aatccactat aaaaaacact gcagcaaatc gtttaaaaac
       61 aagcgtcctt tttcggtcgg gcggaatacg gcggggtcgg tttccagcag gcctttttgc
      121 cttgccgttt cgatttgcgc catgattttg gcactcggta cgcccgtgcg ctcctgcaac
      181 atcgcggtgg gtacgccgtc ggtcaggcgc agggcgttca tcatgaattc gaacggcaaa
      241 tcttcggcgg cgacggtttt gcgttcgacg gcttcgctcg gtcggttttg cattaaggcg
      301 aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga tgcggtcggg ataggaaatt
      361 ttgccgtgcg cgcccgcgcc tatgcctaaa taatcgccga actgccagta gttcaaattg
      421 tggcggcact gcatggctgg tttcgcaaaa gccgatgttt cgtagtggac aaaacccgcg
      481 ctttccagcg cgccgtgtac cgcgtcttcg atgtcgaggg cggcttcgtc ttgcggcaaa
      541 cctttcggcg gcgtatgacc gaacggcgtg ttcggttcca tcgtcagatg atacgcgctg
      601 atgtgggtcg cacccgtagc gatggcggtt tgtacgtcgt ccaatgccgt ctgaacggtt
      661 tggttcggca gggcatacat caagtcgata ttgactttat caaataattt caaggcggta
      721 tcgatagcgg ttaaggcttc cttaccgttg tggacgcgcc ccagccttga gagcatatcg
      781 tcgttgaaac tctgtacgcc aatagaaagc cgcgtgatgc ctgcgtcttt aaatccttga
      841 aacttctcga tttcaaacgt ccccggattg gcttccaacg taatttccgc ttccggctgc
      901 aagcgcaaca gcgaacgcac gccgctcaac aaacggtcaa tcgattccgc ctgaaacagg
      961 ctgggcgtac cgccgccgaa aaagatcgtt tccaccggtc tgccccaaat attgggcaat
     1021 tcaagctgca agtcggtcag cagcgcgtcg atataggcgg cttcgggcaa tccgtttttc
     1081 aggctgtggg aattgaagtc gcaatacggg cattttttga tgcaccacgg gatgtggatg
     1141 taaagcgaca ggggcggcag ggcggtgagt cgggtgcggt ttggaaagga aatggtgtgc
     1201 atggtgtggt tcggaaaagt ggacaatgcc gtctgaaggc ggttcagacg gcacaggttc
     1261 agccgagcag ggtaagcagt tcggcttcgc tgaggatgga aacacccaag gcattggctt
     1321 tttccagctt gctgcccgcg gcttctccag cgacgacgta atcggttttt ttggaaacgc
     1381 tgccggaaac tttgccgcct gcggcttcga ttagggattg ggcttggtcg cgtttgaggg
     1441 tgggcagtgt gccagttaac acgaaggttt tgcccgctac ggctttattg atgccgtctg
     1501 aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa gaaggttttc aggttttcga
     1561 gtagggcggc gttttgtggt tcgctgcgcc atgcttgcca gtcggcgggg agggctttgt
     1621 cgttttgtag accttctatg tttttaccag cgagttccca taaggcttgg gctttgtttt
     1681 cgctaatttt gaagtcgggc aggcgggcga tccagcgttg cggttcggcg tggcgggcgg
     1741 gcgggatggt aacggcttgg gtttgcgggg caacgcctgc ggcgagcagt tcgtctatca
     1801 tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga acgcgccact accgtaccga
     1861 tgtcgggcag gcaggcgagg acgggttcgg gtgcgcggcg gacgcgttcc aatgtgccga
     1921 atgcctgtgc cagcgttttg gcggtgcgtt cgccgacgtg gcggatgccg agcgcgaaca
     1981 ggaagcgcgc gagttcgggc gttttgccgg attctatgcc tgcgaggatg ttttccgccc
     2041 acttgaccgg ttgtttttta tgtttgcccg acgcgccgac cgaactgcct tcagacggca
     2101 tttgatccga ttcggcaacg gttttgtccg ccgtttcctt cattttttgc agggtcggaa
     2161 tatcgaggcg gtagaggtcg gcgaagtgga ggacgaggtc ttgggcgacc agctgttcga
     2221 tttgtttttc acccaagccg tcgatgtcca tggctttgcg cgaggcgaag tggattaagc
     2281 cttgcgcgcg ttgtgcctga caaagcatac cgccgctgca tcgggcgacg gcttcgcctt
     2341 cttcgcgttc gatttcgctg cggcagatgg ggcaatgggt cggcaggcgg tagggcttgt
     2401 ggagcggaac ggattgggtt tgatttgcag ccggtgcttc ggcaaacaaa tcgtcctgcc
     2461 gatgcccgat gccgtctgaa acggcaacgg cggtttcctg catcgggcgg cgttcaaaaa
     2521 tcacacgcac gacttcggga atcacgtctc cggcgcggcg cacgacgacg gtatcgccga
     2581 cgcgcacgtc tttacgcgat acttcgtcct gattgtgcag ggtggcgttg gtaacggtta
     2641 cgccgccgac gaatacgggt tgtaatcggg caaccggcgt taccgcgccc gtcctgccga
     2701 tttgcacgtc aatcgcttcg acaatggtca gggcttcttc ggcagggaat ttgtgggcaa
     2761 tcgcccagcg tggcgcgcgt gagatgaagc cgagttcgcg ctgttgcgcc aagctgttga
     2821 ctttgaccac cataccgtcg atttcgtagg gcagttcggg gcgtttttgc tgcatttgtt
     2881 cgtaaaacgc caatacttcg tcgatatttt tgaaacagcc gaaattgcca tttggcagac
     2941 tgaagccgag tgcttggaaa taggcgagtt cttggatgtg ttcttccgcg acgaaaccgc
     3001 cttgctggcg ggcgacggag taggggaaaa agtgcagttt gcgttgcgca gtgatgcgcg
     3061 aatcgagttg gcgcaggctg ccggcagcgg cgttgcgcgg attggcaaag ggtttttgcc
     3121 cgttttcggc ttgtctttta ttgagggcgg caaaatcggc tttttgcatc agtacttcgc
     3181 cgcgtacttc gatgagttcg ggcgtatttt cgccgtgcag ccgcaagggg atgttggata
     3241 cggttttgat gttttgggta acgtcttcgc ccgtcgtgcc gtcgccgcgc gttgccgcct
     3301 gtaccaatac gccgtcgcgg tagagcaggc tgatggcgag gccgtcgaat ttgggttcga
     3361 taacgtattc gggtttgccg ccgtccaaac cgtcgcgcac gcgttggtcg aaagcgtaca
     3421 tttcggcatg gtcgaacacg ccgttttcat cttgcgggga aaaagcgttg gtcagcgaca
     3481 gcatcggcac ttcgtggcgt acttcggcaa atcccgccaa aggctcgccg ccgacacgct
     3541 gggtcgggct gtcgggcagc ttgagctcgg gatggtttcg ttccaacgct tcgagttcgc
     3601 ggaataattt gtcgtattcg gcatcgggta cgctgggcgc gtcgagggtg tagtattcgt
     3661 aggcatagcg gttgagcagg tcggtgaggt ggcggatgtg ttgtgcggca aattgtttta
     3721 tatcactatc agacggttta agaagattgg taaagttagt gttatgtttt gagtttggat
     3781 tcatgagaga aggttttcag acgacctttg tctgatacgg gatgaaacgg gcaaaggtcg
     3841 tctgaaaaat gataggttga aaacagctga attttacccg aaaaaaagcg gatatgccgt
     3901 aacgacatat ccgctttgat tgcattcgat tttaggagaa caggcgcagg gcggttttgc
     3961 cgcccggttc gataccgact ttgagcatct cggactgacg cgccaataca taagtgcgta
     4021 cgtctttgag ccattgggtc gaaacttctt ccattttgtc gttgaccaga ttcaggttca
     4081 actggccgga caggcgtacc gccaaatcca taaacaaatc gtcgaaggtt ttttcgcctg
     4141 ccggagagtg cgggatgtcg agcagcatac tgaagccttt gtaggactgg ttgtccaaaa
     4201 gggcattggt aaacggctcg ttgttgagcg agcagatgga gaacatggtc gagcccgacg
     4261 tgtcggtata gtggaacgcg ccgtcgtctt ccaaaacgaa acccacgccc gttacggcgg
     4321 aacgcagttc tacgccgctg atgctggtcg gggaaaccaa atggatggcg atggtctggt
     4381 cgacgcgcgc gcagaatgcg tccagcgcgg aagccacttc ggtaaaggcg gcaaggtcgg
     4441 tatgcagcgt ctgaccgccc atgctttgtg cgaatgcgtc cacctggcgg ttgaaggcgg
     4501 agagttcttc ctgcgaggca agtccgttgc ggctgactgc ctgaataccc acgataaatg
     4561 cctgatagcg gatgcccggg atgggttcgg caatctggaa atggtcgtcc atggtgcagc
     4621 cgacaatttg gtagcggcag cggttggaaa ggcgcggcag tgcgtgcagt tctttggctt
     4681 cggtcagcgc gatataagag atgaagtcga agcgcacgtc aaaccagggc agctcgacct
     4741 tcgacagctc tttgagcgta atcagcggtt ttgccggcgt ttgcggaacg ggaacgggtt
     4801 ttgccggcgc gtcggcaggt ttcggtgcgg aatgtccggt ttggggttcg gaaacggtgt
     4861 gggcggagtt gccgataatg ccgctttctt ccaaggcggt ttcgatttcg gttttgaacg
     4921 gggaggcttt tgcctgtttc tgcttggcga tgtagacggc atcctgttct tgcaggttgc
     4981 gcatggcggg gtcttggggt tttgccgttt ttttgaccgc cggttggggt ttcggcatca
     5041 tgactggccc gccggacggt ttgccgtcgc ggacatggct ggttttgctg ttgagcaggg
     5101 catctttgtc ggagtgcccg aactggtcgc gcactttttt gcggtattgg ttttcctgat
     5161 acatattgta ggcgacaacg gcgaggacgg cggcgaggaa cagtacgatg taaatcatgg
     5221 caatcacttt aaatttcggg atgcaggatg cgcaaagtgc gggtactgcg gttaaatcgg
     5281 gcttgcgttt ccggcagtct gacggaacgg ccggttataa cgtttgaatt ataacgaaaa
     5341 ttgcagggtc tgacagcagt gtgtcgaaat aagcggaaat tttccgaaat gccgtctaaa
     5401 atctgtggtt ttcagacggc atttctgtcc acgggaaacc ctttcccccg tatccgccgc
     5461 cagtcgaaaa aatgaccggg gtcggttttg cggccgggcg cgatgtcttg gtgccccgtt
     5521 accgccgtga tggggtagcg gcggcagatt gcgtccaaca aggcttcgag cgaacggtat
     5581 tgcgcttcgg taaagggttc gaaatcgcag ccttccagtt cgatgccgat ggaaaatgcg
     5641 ttgcattttt cccgtccgcg aaacgaggat acgccggcat ggtatgccat attgtcgcag
     5701 gaaacgaact gtaccgtttc gccgtcgcgt ttgattaaaa aatggctgga tacgcgcaaa
     5761 gtgtgtatca ggctgaagaa cggatgtccg tcggggtcga gccggttggc aaacagcttt
     5821 tccaccgcat ccgtaccata ttcgaacggc ggcagcgaga tattgtgcaa tacgacaagg
     5881 gaaacctctt ctttggactc cctcgggccg aaattcggcg acggaatgtg gcgcacgctt
     5941 tgcagccaac cgtcctgcca gggtgccgta gcgggattgt ccatgatgtc ctttctgtct
     6001 tatggggtaa tttgggttat actgtcgccc gaattttaag atgtattccg atattgggaa
     6061 tcctaccatg ttgagaaaat tgttgaaatg gactgccgtt tttttgaccg tatcggcagc
     6121 cgttttcgcc gcgctgcttt tcgtccctaa agacaacggc agggcatacc gaattaaaat
     6181 tgccaaaaac cagggtattt cgtcggtcgg caggaaactt gccgaagacc gcatcgtgtt
     6241 cagcaggcat gttttgacgg cggcggccta cgttttgggt gtgcacaaca ggctgcatac
     6301 ggggacgtac agattgcctt cggaagtgtc tgcttgggat atcttgcaga aaatgcgcgg
     6361 cggcaggccg gattccgtta ccgtgcagat tatcgaaggt tcgcgttttt cgcatatgag
     6421 gaaagtcatc gacgcaacgc ccgacatcga acacgacacc aaaggctgga gcaatgaaaa
     6481 actgatggcg gaagttgccc ctgatgcctt cagcggcaat cctgaagggc agtttttccc
     6541 cgacagctac gaaatcgatg cgggcggcag cgatttacgg atttaccaaa ccgcctacaa
     6601 ggcgatgcaa cgccgcctga atgaggcatg ggaaagcagg caggacgggc tgccttataa
     6661 aaatccttat gaaatgctga ttatggcgag cctggtcgaa aaggaaacgg ggcatgaagc
     6721 cgaccgcgac catgttgctt ccgtcttcgt caaccggctg aaaatcggta tgcgcctgca
     6781 aaccgacccg tccgtgattt acggcatggg tgcggcatac aagggcaaaa tccgtaaagc
     6841 cgacctgcgc cgcgacacgc cgtacaacac ctacacgcgc ggcggtctgc cgccaacccc
     6901 gattgcgctg cccggcaagg ccgcgcttga tgccgccgcc catccgtctg gcgaaaaata
     6961 cctttatttt gtttctaaaa tggacggtac gggcttgagc cagttcagcc atgatttgac
     7021 cgaacacaat gccgccgtcc gcaaatatat tttgaaaaaa taaaccatgc cgtctgaaaa
     7081 gtttgtgttt tcagacggca tacccttacc ggaactgcaa gcatgaaacc gcaattcatc
     7141 actttggacg gcatagacgg tgccggcaaa tccaccaacc ttgccgtcat caaggcatgg
     7201 tttgaacgga gggggctgcc cgtgctgttc acgcgcgaac cgggcggaac gccggtcggt
     7261 gaggccttgc gcgaaatcct gctcaaccct gaaaccaaag ccggtttgcg tgcggaaaca
     7321 ctgatgatgt tcgccgccag aatgcagcat attgaagacg tgatattgcc cgcgctttca
     7381 gacggcatac acgtcgtatc cgatcgtttt accgatgcga ccttcgccta tcagggcggc
     7441 gggcggggga tgccgtctga agacattgaa attttggaac attgggtgca gggcggtttg
     7501 cgccccgatt tgaccctgct gttggatgtg ccgctggaag tgtcgatggc gcgtatcggg
     7561 cagacgcgcg agaaagaccg attcgagcag gaacaggcag atttcttcat gcgtgtgcgc
     7621 agcgtttatc tgaaccgtgc cgccgcctgt cccgaacggt acgccattat cgacagtaac
     7681 cgcagcttgg atgaagtcag aaacagcata gaaaaagtgt tggacggaca tttcggctgt
     7741 taatgcggca aatattgaaa caagcgcatc cacccgcgcc gaaaatcaaa cggcagtgcc
     7801 gcaggtgaaa atggcggtat gcgccaaact ttcggcatga tagaattacg ctcggttaca
     7861 aggcaggatg cgtcggcaat attaacgaac cgcccgtaac atgatgaccc gaaagcgttt
     7921 cggacagtcc gattcaaatc tttttctcgc aacaggattg acacatggaa aactcattga
     7981 aagaagccgc cctcaagttc cacgaattac ccgttccggg caaaatttcc gttaccccga
     8041 ccaaatcttt ggcgaccgac aaagatttgg cgttggcgta ctctccgggc gtagccgctc
     8101 cttgtatgga aatccatgcc gatccgcaaa atgcctacaa atacaccgcc aaaggcaact
     8161 tggtcgccgt catttccaac ggtacggccg ttttgggctt gggcgacatc ggcgcgctgg
     8221 cgggcaaacc cgtgatggaa ggtaaaggcg tattgttcaa aaaattcgcc ggtgtggacg
     8281 tgttcgacat cgaaatcgat gaaaaagacc cgcaaaaact cgtggacatc atcgccgctt
     8341 tagagccgac cttcggcggc atcaacctcg aagacatcaa agcacccgag tgtttctaca
     8401 tcgaacgcga attacgcaaa cgctgcaaaa tccccgtatt ccacgacgac cagcacggca
     8461 cggccatcat taccgccgcc gccgtattga atgccctgcg ttttaccggc cgtaaaatcg
     8521 aagaagcgac tttggtgtgt tccggcgcag gtgcggccgc gattgcctgc ttgaaccaat
     8581 tgctggattt gggcttgaaa cgcgaaaacg tgaccgtttg cgactccaaa ggcgtgattt
     8641 accaaacccg cgaagacaaa gaccgcatgg atgagtctaa acagttctac gccattgaag
     8701 acaacggcca gcgcgtgctt gccgatgccg tcaaaggcaa agacatcttc ttgggcctct
     8761 ccggcgcgaa cctgctgacg cctgaaatgc tgaacaccat gaacgaaaaa cccatcgtgt
     8821 tcgccatggc caacccgaat ccggaaattc tgccgccgct ggcaaaagaa acccgtccgg
     8881 acgtggttat cggtaccggc cgttccgact tcccgaacca agtcaacaat gtattgtgct
     8941 tcccgttcat cttccgtggc gcgttggatg tcggcgcgac caccatcaac gaagaaatga
     9001 aacgcgcctg cgtgtatgct ttggcggatt tggcgatgga agaagtgacc gaagaagtgg
     9061 ttgccgctta cggtaagaaa tttgaattcg gcgcggaata cctgattccg actccgttcg
     9121 attcccgcct gctgccgcgc gtcgctacgg ctgccgccaa agcagcgatg gaaagcggcg
     9181 ttgcaacccg tccgattgcc gactttgaag cctacgccgc caagctgggc gaatggaagc
     9241 tgtaagccgt ttgcggttta aaatgccgtc tgaactgttt tcaggcggca ttttgctgtc
     9301 agattgataa atgaaagata ctggaaaatg aaagagatga aacctgtccg ttatcatatt
     9361 ggcgatatgc ccgagacttc aaaacaaacc gcctcccgtc atgacgacag ggcagtgggt
     9421 gttgacgatg attgttttca tgattccttt ggtcaatttt tgtttgggtg ttcggcagag
     9481 gcaacccgaa ccgcgccaat ttctgtaaag cgcagttgct tatttacctg attggttcgc
     9541 ttatcggttt ggtcttcgcg ttgtttatag gtgggtctgt atcaggtacg catgattaat
     9601 gccccgggct gattttgctt cgaggatttg tatcgaatat gccgaattgt ttcaaatttc
     9661 ataccgttat cgaacggcat tggcaaaaac cttatccggt tttgtctttt ctgcttaagc
     9721 cgctctccgg gctgtttgcc aaaattgcgg caaaacggcg gacggatttt ttatcgggaa
     9781 aacggcaaag cgaaaagctg cccgtgcctg tggtcgtggt cggcaacatc cacgcgggcg
     9841 ggacggggaa aacgccgatt gttgccgcgc tggtgtcggg tttgcaggaa aagggcgtca
     9901 aggtcggcat catcagccgg ggctacgggc gcaagagcaa ggcggttcat gtattgaatg
     9961 ccgcaagccg agcggaagat gcgggcgacg agcctttgct gctgttcagc aaaaccggtg
    10021 cgccgacggc ggtgggcagc agccgtgcag aggtaggcag ggcattgctg gcggcgcatc
    10081 ccgacatcgg actgattgtg gcggacgacg gtttgcagca ttacgccctg cggcgagatg
    10141 tggaaatcgc ggtgtttccg gcggcggata cggggcgcac ggatttggat ttgctgccca
    10201 acggcaattt gcgcgaacct ttgtcgcggc tggattcggt ggatacggtc gtcgtcagcg
    10261 gcggcaaggc ggatgcgctg tttaggccgt ctgaaaatat gtttcacagc cgtatcgaag
    10321 cgggacgaat ttaccgtttg aacaatccgt ccgaaatact ggacacaggc cgtctgaaaa
    10381 atcaaaccgt cgtcgccgtg gcaggtattg ccaagccggc gcgttttttc gattcgttgc
    10441 ggaatatggg cattaccgtg aagcgaaccg tcgcgctgcc cgaccacgcc gacatttcgg
    10501 cggcagattt gcccgatgcg gacgcggtca ttattacgga gaaagatgcg gtcaaatttt
    10561 cagacggcat ttgcaccgat aatgtttggg tgttgcccgt ttgtgcgata atcgaacctg
    10621 atttggcggc gtttgtgttg gagcggttgg aagatgtacc gaaggccgtc tgaaagcacg
    10681 gtttgggcgg agtgattacg gatttgaata agaacgcctc gcgccgtcat tcccgcgcag
    10741 gcgggaatct aagtctcgaa ttttcaggaa tgcctaggag gcttcagaaa tcccaaatct
    10801 ccggatttcc acttggacag gaatgagaaa atcggtcgta ttttttatct gcattaatca
    10861 ttcgttaaag gattgaatat taaactgaaa accttgttat tgcccttcgc cacgctggca
    10921 ttgtgcacca acgcttttgc cgccccgccc agcgacgcgt cgttggcgcg ttggctggat
    10981 acgcagaatt ttgaccggga tatagaaaaa aatatgattg agggctttaa tgccggattt
    11041 aaaccgtatg cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca
    11101 gaagccttta accgttatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa
    11161 caggctgtcc gtaatacttt attgaagaat gcccgtgaga tatacacgca agaagaaatt
    11221 gacggcatga ttgcctttta cggttcgcct gtcggtcagt ccgtcgttgc caaaaatccg
    11281 cgcttaatca agaaatcgat gagtgaaata gcagtatctt ggactgcatt gtcagggaaa
    11341 atcgcgcgac atcatctgcc cgcgtttacg gaagagttgc ggcgcatcat ctgcggtggt
    11401 aaaaatcccg atgcgggctg taaacaagcc ggacaggttg ggaaaaggca tcagaaataa
    11461 atgatagccg tctgaaatat tgaagagggc atccgattga ttgaaccatc aaacccgaaa
    11521 gcaaccctat ggaaaaaaaa ttcttagaca tcctcgtctg ccccgttacc aaaggcaggc
    11581 tggaatatca tcaggacaaa caggaattgt ggagccgtca ggcgaagctt gcctatccga
    11641 ttaaagacgg cattccctat atgctggaaa acgaagcgcg aacgttgagc gaagaggaac
    11701 tcaaagcatg accgaattcg tcgtattgat tccggcgcgg ctggattcgt cgcgcctgcc
    11761 cagaaaagcc ttggcggaca tccacggcaa accgatggtc gtgcgcgttg ccgaacaggc
    11821 ggcaaaaagt aaagctgcgc gcgtcgttgt tgccaccgac catcccgata ttcagacggt
    11881 ctgtcaggcg cacggcgtcg aagtcgtcat gacttcaaac cggcacgaaa gcggcacgac
    11941 gcgccttgcc gaagcctctg ccgcgctgaa gctgccgccg catctggtgg ttgtcaacgt
    12001 acagggcgac gagccgctga ttgcccccga actcatcgac cgcactgccg aagtcttggt
    12061 ggaaaacaac gtacagatgg caactgcggc acacgaattg cacgatttcg acgaattgat
    12121 gaatcccaat gccgtcaaag tcgtcctcga caaaaaccgc aacgccatct acttcagccg
    12181 cgccccgatt ccctatccgc gcgatgcgat gcgtgccgga aaacgcgaaa tgccgtctga
    12241 aaccgccgtc ctgcgccata tcggcatcta cgcttaccgc gccggcttcc tgcaacgcta
    12301 tgccgaaatg agcgtttcgc cgctggaaac catcgaatcg ctggaacagc tgcgcgtcct
    12361 gtggcacggt tatcccattg ccgtcgaaac cgccaaagaa gcccccgccg ccggtgtgga
    12421 tacgcaggaa gatttggata gggttcgcgc tgtatttcag accgtataaa acaggttcaa
    12481 agggaaaaga tatgcagcaa catattgaaa agtggcaaca cttgagccgg gaagaacaga
    12541 aaatccttgc cgaagtatgg ggtctcgtgc aaaacgatga tcaggaggtt cactatgaaa
    12601 tgcttaaatt gaacgcaccc gacgaagcca gtggcgaatt ttggttcaga atggcagaaa
    12661 cactcagcac cctgccgccc aaccgttccc tcgaccttag aatgaacggc ggcaggctgg
    12721 cgaccgccgt atccatcctt tccgtcatga ttgaagacaa tcccgacata ccgcagcttt
    12781 gggcgcaaaa aattaccgcg ctcaattata gtggattaaa tttaaaccag tacggcgttg
    12841 cctcgccttg tcgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    12901 taatccacta tatttggcac acggacacaa agcccgtgcc gacggtttgg cacaacagcc
    12961 cgacaaagcg gcagaagcca acgaggagga atacctgacc aaagccctgt cgcaaaacct
    13021 gctgtcaaca ttggatgtcg cgcttgcacg ttttcctgaa gacgcgtggt ttcaggaaat
    13081 caaacaggat gcacaaaagc attttgcttg aggatgtggc agtcaggaat atttccattc
    13141 aggaagaaaa gaagtgcctg attgggtata atcagggtaa atcttatttt atttcaaaag
    13201 attaatattt gctttctgtt tttccttgac ggtatcggaa aagttgatta tagttacagc
    13261 ttccttagga gtaatggctg agaggctgaa ggcacttccc tgctaaggaa gcatgtgggg
    13321 tcaacctgca tcgagggttc gaatccctct tactccgcca gataaaaaat agacgctgtg
    13381 ttttacagcg tctatttttt atgcaatttt atagcgggtt ggtgcaaaac cagtatggta
    13441 ttgccgtgtc ttgattctga attttgttat agtggattaa caaaaaccag tacggcgttg
    13501 cctcgcctta gctcaaagag aacgattctc taaggtgctg
//
LOCUS       DAWKOD010000042        13483 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000042 DAWKOD010000000
VERSION     DAWKOD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.042"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..2748
                     /gene="mfd"
                     /locus_tag="WJK73_000918"
     CDS             <1..2748
                     /gene="mfd"
                     /locus_tag="WJK73_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7557417.1"
                     /translation="TNPLPSPLPQEREQSAAVVSDSLKAAAVSTESRLPPGKSNLHGQ
                     IQQQPTPSPVGEGWGEGKAVAAQTEFSATATNPLPNPLPHEREQSAAAVSDDLKAKSS
                     LHLVASNLHRQIRQQPTPSPVGEGWGEGKAVAAQTEFSASATNPLPSPLPQEREQSAA
                     AVSDDLKAKSSLHLVASNLHGQIRQQPAPSPVGEGWGEGKAVADQSAIAVITESDLYQ
                     YVARSRIHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMNLGGETNE
                     MMLLEYAGEAQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTA
                     AELLNLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMD
                     RLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVA
                     SLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQK
                     EQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLSVKTFVKPFSEGS
                     VREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMR
                     DFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFT
                     LYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLA
                     VCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTF
                     GKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLP
                     K"
     gene            2897..3280
                     /locus_tag="WJK73_000919"
     CDS             2897..3280
                     /locus_tag="WJK73_000919"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ7557418.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            3302..4144
                     /gene="kdsA"
                     /locus_tag="WJK73_000920"
     CDS             3302..4144
                     /gene="kdsA"
                     /locus_tag="WJK73_000920"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ7557419.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            4159..4599
                     /locus_tag="WJK73_000921"
     CDS             4159..4599
                     /locus_tag="WJK73_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ7557420.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMAAALLSIYLSWRLSGKDAV"
     gene            4647..5933
                     /gene="eno"
                     /locus_tag="WJK73_000922"
     CDS             4647..5933
                     /gene="eno"
                     /locus_tag="WJK73_000922"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ7557421.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            5947..6225
                     /gene="ftsB"
                     /locus_tag="WJK73_000923"
     CDS             5947..6225
                     /gene="ftsB"
                     /locus_tag="WJK73_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ7557422.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(6266..6556)
                     /locus_tag="WJK73_000924"
     CDS             complement(6266..6556)
                     /locus_tag="WJK73_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ7557423.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(6754..7887)
                     /gene="nrdB"
                     /locus_tag="WJK73_000925"
     CDS             complement(6754..7887)
                     /gene="nrdB"
                     /locus_tag="WJK73_000925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ7557424.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(7948..9135)
                     /locus_tag="WJK73_000926"
     CDS             complement(7948..9135)
                     /locus_tag="WJK73_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ7557425.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(9128..10141)
                     /gene="dcm"
                     /locus_tag="WJK73_000927"
     CDS             complement(9128..10141)
                     /gene="dcm"
                     /locus_tag="WJK73_000927"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7557426.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(10199..12478)
                     /gene="nrdA"
                     /locus_tag="WJK73_000928"
     CDS             complement(10199..12478)
                     /gene="nrdA"
                     /locus_tag="WJK73_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ7557427.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            13007..13318
                     /locus_tag="WJK73_000929"
     CDS             13007..13318
                     /locus_tag="WJK73_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ7557428.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
BASE COUNT         3563 a         3531 c         3052 g         3337 t
ORIGIN      
        1 acaaaccctc tccctagccc tctcccacag gagagggaac aaagtgcagc cgtcgtttca
       61 gacagtctga aagcagccgc cgtttcaacc gaaagccgcc tgccccccgg taaaagtaat
      121 ctgcacgggc aaatccaaca gcaacctact ccctcccccg tgggggaggg ttggggagag
      181 ggcaaagcag ttgccgctca aactgaattt tccgcaaccg caacaaaccc tctccctaac
      241 cctctcccac atgagaggga acaaagtgca gccgccgttt cagacgacct gaaagccaaa
      301 agcagcctgc atctcgtcgc aagtaatctg cacaggcaaa tccgacagca acctactccc
      361 tcccccgtgg gggagggttg gggagagggc aaagcagttg ccgctcaaac cgaattttcc
      421 gcatccgcaa caaaccctct ccctagccct ctcccacagg agagggaaca aagtgcagcc
      481 gccgtttcag acgacctgaa agccaaaagc agcctgcatc tcgtcgcaag taatctgcac
      541 gggcaaatcc gacagcaacc tgccccctcc cccgtggggg agggttgggg agagggaaaa
      601 gcagttgccg accaaagtgc catcgccgtc attaccgaat ccgacctcta ccaatacgtc
      661 gcccgttcgc gcatccacaa ccgccgcaag aaacacgccg ccgtttcaga cggcctgttg
      721 cgcgaccttg ccgaaatcaa tatcggcgat cccgtcgtgc acgaagaaca cggcatcggg
      781 cggtatatgg gcttgatcac aatgaatttg ggcggcgaaa ccaacgaaat gatgttgctc
      841 gaatacgcag gcgaagcgca gctttatgtg cccgtttcgc aactgcattt aatcagccgc
      901 tactccggcc aggcgcatga aaatgtcgcc ctgcacaagc tcggcagcgg cgcgtggaac
      961 aaggcaaaac gcaaagccgc cgaaaaagcg cgcgataccg ccgccgagtt gctcaacctc
     1021 tacgcccaac gcgccgccca atcgggacac aagtttgaaa tcaacgagtt ggattatcag
     1081 gcgtttgccg acggcttcgg ctacgaagaa accgaagacc aggcagccgc catcgccgcc
     1141 gtgattaaag atttgacgca ggcgaagccg atggaccgcc tcgtgtgcgg cgatgtcggt
     1201 ttcggcaaaa ccgaagtcgc cctgcgcgcc gcgtttgtgg cggtaatggg tggcaaacaa
     1261 gttgccgtac tcgccccgac cacgcttttg gtcgagcagc acgcgcaaaa cttcgccgac
     1321 cgtttcgccg atttccccgt gaaagtcgcc agcctttcgc gtttcaacaa cagcaaagcc
     1381 accaaagccg cgctggaagg catggcagac ggcacggtcg atattgttat cggtacgcac
     1441 aaactggtgc aggacgacat caaattcaaa aacttaggtt tagtgattat cgatgaagaa
     1501 caccgcttcg gcgtgcgcca gaaagagcag ctcaaacgcc tgcgcgccaa tgtcgacatc
     1561 ctcaccatga ccgccacacc gattccgcgc actttaagta tggcgttgga aggactgcgc
     1621 gacttctcgc tgattaccac cgcgcccagc cgccgcctct ccgtcaaaac ctttgtcaaa
     1681 ccctttagcg aaggcagcgt gcgcgaagcc gtattgcgcg aactcaaacg cggaggacag
     1741 gtatttttcc tgcacaatga agtggatacc atcgaaaaca tgcgtgagcg gctggaaacc
     1801 ctgctgcccg aagcccgcat cggcgtggcg cacggacaac tgcgcgagcg cgagctggaa
     1861 caagtcatgc gcgacttttt gcagcaacga tttaacgtgt tgctctgttc caccatcatc
     1921 gaaaccggta tcgacattcc caacgccaac accatcatca tcaaccgtgc cgacaaattc
     1981 ggattggcgc agctgcacca gcttcgcggg cgcgtcggac gcagccacca ccaagcctac
     2041 gcctacctgc tcacgcccga atacatcacc aaagacgcag aaaaacgcct cgacgccatc
     2101 gcggcagcag acgaactcgg cgcaggtttc accctcgcca tgcaggattt ggaaatccgc
     2161 ggcgcaggcg aaatcctcgg cgaagggcag tccggcgaaa tgatacaggt cggcttcacg
     2221 ctctacaccg aaatgctcaa acaagccgtg cgcgatctca aaaaaggccg ccaacccgac
     2281 ctcgacgcgc cgctgggcat caccaccgaa atcaaactgc acagccccgc cctactgccc
     2341 gaaagctact gccccgacat ccacgagcgt ctcgtcctct acaaacgcct cgccgtctgc
     2401 gaaaccgtgc agcaaatcaa cgccatacac gaagaactcg tcgaccgctt cggcctgccc
     2461 gaacaacctg tcaaaaccct tatcgaaagc caccacctgc ggcttatggc aaaagaattg
     2521 ggtatcgatg ccattgatgc ggccggcgaa gcggtaacgg taaccttcgg taaaaacaat
     2581 aatgtcgatc caaccgaaat catcctgctg attcagaacg acaaaaaata ccgccttgcc
     2641 ggcgccgata agctgcggtt taccgcagag atggaaaata tcgaggtcag aatcaacacc
     2701 gtaaaaaacg ttttaaaaac cttgcaaaac agatgcctgc ccaaataaag ccgacaccgc
     2761 aatgccgtct gaaacaccgt tttccttgtc cgaaagccgc cattatgaat ttgaaggaaa
     2821 ctccactata atacggcatt cagatttcca gacggcatcg cgcccgtcaa accgcacaca
     2881 aaccaaaagg aaatacatgt tccgtactat gcttggcgga aaaatccacc gcgccaccgt
     2941 taccgaagcc gatttaaact atgtcggcag tattaccgtc gatcaagatc tgttagacgc
     3001 ggcaggcatc taccccaacg aaaaagtcgc catcgtcaac aacaacaacg gcgaacgttt
     3061 tgaaacctat accattgcag ggaaacgcgg cagcggcgtg atttgtctga acggtgctgc
     3121 ggcaaggctg gtgcagaaag gcgatatcgt catcatcatg tcttacgtcc aactctccga
     3181 acccgaaatc gccgcacacg aacccaaagt cgtcttggta gacggaaaca acaaaatccg
     3241 cgacatcatc tcctatgagc cgccgcacac cgtgctgtaa ccctgaaaac ggacattgac
     3301 catggatatt aaaatcaacg gcattaccgt cggtaacgac gctccttttg tactgttcgg
     3361 cggcatcaac gttttagaag atttggattc caccctccaa acctgtgcgc attacgtcga
     3421 agttacccga aaactcggta ttccctatat ctttaaagcc tctttcgaca aggcaaaccg
     3481 ttcctccatc cattcttatc gcggcgtagg cttggaagaa ggcttaaaga tttttgaaaa
     3541 agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc atcagtgcca
     3601 acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgca ttccttgcgc ggcagaccga
     3661 tttagtggtt gccatggcaa aaaccggcaa cgtcgtcaac atcaaaaaac ctcagttcct
     3721 cagcccctct caaatgaaaa acattgtgga aaaattccac gaagccggca acgggaaact
     3781 gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg atatgctcgg
     3841 tttcggcgtg atgaaacaga cttgcggcaa cctgccggtt attttcgacg ttacccattc
     3901 cctgcaaacc cgcgatgccg gttctgccgc atccggcggt cgtcgcgcac aggctttgga
     3961 tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat cgcaccccga
     4021 tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctgcaccttt tagaagattt
     4081 tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt taacaatcga
     4141 gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta tgccgcaacc
     4201 ttgtggctcg gtatgcagat tatggcgggt tatatcgccg cgccggtgct gttcaaaatg
     4261 ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct ctcttggagc
     4321 gggcttgccg tttggggcgc ggtactggct gccgcctttg ccgccctaac ccggcggcaa
     4381 accgccctgc tgcttttttt attgtccgcc cttgccgcca accaattttt ggttaccccc
     4441 gttatcgagg cactgaaata cgggcatgaa aattggctgt tgtcgtttgt aggcggatcc
     4501 ttcggaatgt ggcacggtat ttccagtatt gtttttatgg cggccgccct actttcgatt
     4561 tacttaagtt ggcggctttc cggcaaagat gccgtctgaa gccctcccat ttttttacct
     4621 cccttcactt cacttggaga acattcatga gcgcaatcgt tgatattttc gcccgcgaaa
     4681 ttttggactc acgcggcaac cccacagtcg agtgtgatgt attgctcgaa tccggcgtaa
     4741 tgggacgcgc agccgtaccg agcggcgcgt ccaccggtca aaaagaggct ttggaacttc
     4801 gcgacggcga caaatcccgt tattcgggca agggcgtatt gaaggcggtc gaacacgtca
     4861 acaaccaaat cgcccaagcc ctcattggta tcgatgccaa cgagcaatct tatatcgacc
     4921 aaatcatgat cgaattggac ggtactgaaa acaaaggcaa tttgggtgcg aatgcgactt
     4981 tggcggtttc tatggcggtt gcacgcgccg ctgccgaaga ctcaggcctg ccgctttacc
     5041 gctacttggg cggcgcaggc ccgatgtccc tgcccgtacc gatgatgaac gtcatcaacg
     5101 gcggcgaaca cgccaacaac agcctgaaca tccaagagtt tatgattatg cccgtcggcg
     5161 caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat tttccacgcc ttgaaaaaac
     5221 tgtgcgacag caaaggcttc ccgaccacag tcggcgacga aggcggtttc gcccccaacc
     5281 tgaacagcca caaagaagcc ctgcaactga tggtcgaggc gaccgaagcc gccggctaca
     5341 aagcgggcga agacgtatta ttcgcattgg actgcgcgtc cagcgagttc tacaaagacg
     5401 gcaaatacca cttggaagcc gaaggccgct cctacaccaa cgcggaattt gccgaatatc
     5461 tggaaggcct ggtcaacgag ttccccatca tctccatcga agacgggatg gatgaaaacg
     5521 actgggaagg ctggaaactg ctgaccgaaa aactgggcgg caaagtccaa ctcgttggcg
     5581 acgacctctt cgttaccaac ccgaaaatcc ttgccgaagg cattgaaaaa ggcgtggcaa
     5641 acgcactatt ggtcaaagtc aaccaaatcg gtactttgag tgaaaccctg aaagccgtcg
     5701 acttagccaa acgcaaccgc tacgccagcg taatgagcca ccgctccggc gaaaccgaag
     5761 acagcaccat tgccgacttg gcagtcgcca ccaactgtat gcagatcaaa accggttctt
     5821 tgagccgttc cgaccgcatg gcgaaataca accaactgct gcgtatcgag gaagaattgg
     5881 cggaagccgc cgactacccc agcaaagccg cattctacca actgggcaaa taaaaaaggt
     5941 taaggtatga agtgggtaac tgtcgtttta tccttcgcac ttgtctgttg ccaatacagc
     6001 ctctggttcg gcaaaggcag catcggacgc aacagcagtc tgagagaaca gattgccgtt
     6061 caagaagaaa aaaaccagac actcgcccta cgcaatcatt cccttgccgc cgaagtctat
     6121 gatttggaaa acggtcaaga agccatttcg gaaatcgccc gggtagaact gggttatatc
     6181 caagacggtg aaacctttta ccgactcatc aggcataacc ggtaataccg tcaaaaagcc
     6241 gtccgaacca atgttcggac ggcttttatt tcaacaaact gtcagacagc ccctcatcct
     6301 cccccgacaa accgcaatcc agcctgacat ccccctcgac gcaacagcag cacggcagta
     6361 tctcgtcccg ccccaaaaaa gccaaaggcg gctcccgata agtaacgctt ccctccaaaa
     6421 tcttcactcg gcacgatccg caatatccgc ttcggcactg atattccacc atatgccccg
     6481 tccgttccaa gccttccaac agggtctcgc cctccaagag ctcaaaaaaa cccttattcg
     6541 taccaatgcg cgccatttcc gaccaatcaa aaatatagtg gattaaattt aaaccagtac
     6601 ggcgttgcct cgccttgtcc ggatttttgt taatccacta taatccacta taaaaggaac
     6661 aataaccgat cctacccgct gtttttccca tcatacaaca tacaaatgcc gtctgaaaca
     6721 tccggcttca gacggcattt tttcaaaaaa cgtttacaac tcaaaatcgc ccaaatcatc
     6781 cgtattcact tcagaatcga tctgaccgat caaataagag gatatttcca cttcctgcgg
     6841 cgcgacctgt acgttatcgg acgacagcca cgcattaatc cacggaatcg ggttttgatt
     6901 tgcgccttca aatccggccg gcaaccccac cgcctgcata cgcagattgg taatatattc
     6961 gacgtactgg gataagattt ctttgttcaa accaatcatc gaaccgtctt taaacaaata
     7021 tgccgcccat tctttttcct gttccgccgc ttttttgaag agttggaaac attcgtcctg
     7081 caactcagcg gcaatttctg ccatttcaga atcatcgaca cccgaacgca tcagattaag
     7141 catatgctgc gtgccggtca ggtgcagggc ttcgtcgcgg gcaatcagtt tgatgatttt
     7201 ggcgttgcct tccatcaact cgcgctcggc aaaagcaaac gagcaggcga atgaaacgta
     7261 gaaacggatg gcttccaaca cgttgacgca catcaggcag agatagagtt ttttcttcaa
     7321 cccgcgcaaa gacacagtaa tgggttttcc gccgacattg tgcacccctt cgcccaacag
     7381 attgtaatac tgggtgtatt cgattaagtc gtcgtaatag caggcaatgt cttcggcgcg
     7441 ggcggtaatg tattcgtttt cgacaatatc atcaaacacg accgacggat cattcacaat
     7501 attgcggatg atgtgggtat agctgcgcga gtgtatggtt tcgctgaagc tccacgtttc
     7561 aatccacgtt tccaactcgg gaatcgaaac caaaggcagc aaggcaacat tcggactgcg
     7621 cccttggatg gaatcgagca gtgtttggta tttcagattg ctgatgaaaa tatgtttttc
     7681 gtgttctggc aggttggcgt agtcgatacg gtcgcgcgac acgtcgattt cttccggccg
     7741 ccaaaaaaac gacaattgtt tttcaatcag tttttcaaat acctcgtatt tctgctggtc
     7801 ataacgggcg acattaaccg gctgaccgaa aaacatcggc tcattcagcg cgtcgttttt
     7861 ggttttggga aaggtgctgt atgacatagt taagtgttcg caggacataa tctattctct
     7921 atattaatta aaaatgatct gtaagtttta actaacctgc tgagacttta tatatttaat
     7981 aaaattttct aaagttaaaa catgggtttt cccaaaagtt ccaaatgttt ttacggattc
     8041 tttagggaca actaaggtta aattctcatc actcatttcc tgaatttgag tatcagatat
     8101 accttcttgt agagtaaata aatgcttatg aggaattcga tctgcttcat ttaaaacttg
     8161 gcgccagcga tccttacaag tagtctttga acctagcatt gtcagctttt cagtttgaaa
     8221 tatgaaattc cctgattcat ccatcgcatg gtactcttca cttccaggaa ataaaaaatc
     8281 tggtttcttt ttcccttctg tcttcgcttg cgtctcaaat tttaaggaaa attcgtcaaa
     8341 tattcttgat aaatgtaact ctaaagattt tcctgctctt gcttttctgc gatttgtaaa
     8401 agaatgtgca aaattaatga agctatccaa atcagtaatt tttgatttaa taatttcaaa
     8461 ttcacgtttt tcaaaaatgg aaaataattc atattccttt gaaacccatt gttttaacac
     8521 attactaacg ttagttttat ttaatgcaat attttccctt gccaaaactg ccatttcttc
     8581 agttttgggg aagttaggaa atagtgaaaa taattttgtt aggttatcct ctacttcttg
     8641 ttttttaggt aaatataaac ttcctggtaa aatgttagtt tctgcgataa atatttcaat
     8701 atcttcatct gaagataaaa caaaagcatg gaataataaa tctttataac aagctctgca
     8761 caataccaat aaggaaccac tatatttatc acttaaaaat tcaaaattct tgccaaaacc
     8821 cgtaattctc gcctcatttc tcgtgccttg gccgtaatat ttaaaattgc tattagtgac
     8881 gcttccatct tgccaattaa ttttgattga aatagtttta tttgttccct tttggcaaac
     8941 tacatcaaag aggtcgtctg aaaaatgttt ttgaatataa aatcctgatt ggtgactacc
     9001 agtagtacca acatcattgg gacgaatata acgacagtat actgcaattg atttatttgc
     9061 tgtctgtatt gctgaagcta ctaagtcatc ggatttattt actacttgta ttgtctgagc
     9121 tgccaagtca ttcatttttt aactcattta taatttgttt agcaatagct tgaaccaacg
     9181 gtacagcaat tgaattgcca aactgcttgt atgcagctgt cttggatact gcatcaataa
     9241 caaaatcttt aggaaatccc attaaacgcg agcactccct aggtgtcagc ttcctaggat
     9301 tttttccttt ctgagggatg agtatttcgg aaccatcttt gtaatatcgt gcagatagag
     9361 ttcgtgatat tccatctaaa tcaactaatc caaaaccaaa tccattaccc tttgccttat
     9421 gttttttagc gtaattttga aggtaaagcc ataagttatc agaaagagta aaagaattat
     9481 ctacatcatc ttccaaaatt tgctttaatt ttggttgtga ttctggtggg gaaggaaaat
     9541 tgaaatttat ttccttatta aaatattgtc tatcaaaacc tacaataaaa atacgctccc
     9601 tattttgagg aacataatat tttgcattca taacttgata aaatatctga tagtcaagct
     9661 cttctaaagt ccctttaatt actttaaatg tatttccttt gtcatgcgaa acaaggtttt
     9721 tcacattctc taaaagaaaa attttaggtc gatgttttcc aataatttca gcaacatcaa
     9781 aaaatagagt tccctgcgcc ttatctaaga agcctgtttc tcgtcctagg ctttttttct
     9841 ttgaaacacc agctatagag aatggctgac acgggaatcc tgctgttaat acatcaaact
     9901 tacttggaat agctgctttg gtttccttta atgtaatatc tccataagga atatcattaa
     9961 aatttacttg gtaggtttga cgggctttat catcccattc actagaaaat acacatcgcc
    10021 caccaacatt ctccattgca atgcgaaaac cacctattcc cgcaaataaa tcaataaaag
    10081 taaatttctc attatttaag gtaactatat tatctaattg attttgtatt ttatttttca
    10141 tattttatat ttcagatgat ttattaatct aaaggccatc tgaaaactcc ccctttcatc
    10201 aaatcttaca agccccgccc gcgcagccgt catcctgaat atcggtctgc gtatcgtccg
    10261 cgccgtcgcg ggtgttatgg tagtacaggg ttttgacgcc gtatttgtag gcggtcagca
    10321 ggtctttgag catttgtttc atagaaactt tgccgccttc gaatttgccc gggtcgtagg
    10381 cggtgttggc ggaaatcgcc tgatcgacga atttttgcat cacgccaaca agtttcagat
    10441 agccttcgtt gccgggaagc tgccacaggg tttcataggc atttttcagg gtttcaaact
    10501 ccggcacgac ttgtttcaaa atgccgtctt tcgatgcttt gaccgttacc aatccgcgcg
    10561 gcggctcgat accgttggtg gcgttggcga tttgcgaact ggtttcagac ggcatgagtg
    10621 cggttagagt agagttacgc aggccgtatt tgacgatgtc ggcacgcagg ctttcccaat
    10681 cgtagtgcaa aggctcgtta cacacagcgt ccaagtcttt tttgtaggtg tcgatgggca
    10741 gtttgccttg cgaataaacg gtttggttaa agagcgtgca cgcaccgtat tctttggcaa
    10801 ggtttgccga tgctttgagc aggtaatact gtatggcttc aaaggtacgg tgggtcagac
    10861 cgagcgcgga gccgtcgctg tagcggacac cgtttttcgc caaataatac gcatagttga
    10921 tcacgccgat accgagcgaa cggcggccca tagtggaggt acgcgcggct tctaccggat
    10981 atccctgata atctaaaagt gcatcgagcg cgcgcacggt caaatcggca agcccttcca
    11041 attcgtccaa gctgtttaat gcccccaagt tgaaggcaga cagggtacac agggcgattt
    11101 cgccgttcgg gtcgttgata ttgtccaaag gtttggtcgg cagcgcgatt tccatacaca
    11161 agttggactg atgaacaggc gcaacgcgcg gatcgaacgg gctgtgcgta ttgcagtgat
    11221 cgacgttttg aatgtagatg cgcccggttc cggcacgctc ctgcatcagc gtggaaaaca
    11281 ggtcggcagc cggaatgatg cgcttgcgga tatcagggtc ttgctcgtat ttcgtataga
    11341 gccgctcaaa ttcgtcttgg tcggcaaaaa acgcttcgta caatcccgga acctcgttgg
    11401 gcgaaaacag cgtaatgttg ccgcccttaa tcaggcgggt gtacagcagg cggttgattt
    11461 gcacgccgta atcaagctga cggatacggt tgtcttccac gccccggttg tttttcaaca
    11521 ccagcaggct ttcggcttcg atatgccaca aggggtagaa caaggttgcc gcgccgccgc
    11581 gcacgccacc ttgcgaacag gatttgactg ccgcctgaaa cattttaaag aagggaatgc
    11641 agccggtatg ccgtgcttcg ccgccccgga tttcgctgtc cagtccgcgg atacgtccgg
    11701 cattgatgcc gatgcccgca cgctgggaaa cgtatttcac aatcgcgctg gtggtggcgt
    11761 tgatggaatc caggctgtcg tcgcattcga tcaatacgca gcttgagaac tggcgcgtag
    11821 gcgtacgcac gccgctcata atcggggtcg gcagcgatac tttaaatgtg gaaacggcat
    11881 cgtaaaaccg tttgacgtaa tcgaggcgtg tttctttggg atatttgctg aaaagacaca
    11941 tcgccaccag aacatataag aactgcggtg tttcataaat ctggcgggta acgcggttct
    12001 gtaccagata tttgccttcg agctgtttga cagcggcata ggaaaaggac atatcgcgtt
    12061 cgtggtcgat ataggcgttc agctcgtcaa attcttcgcg gctgtaatcc tcaaggatat
    12121 gcctgtcgta ttttgcggca tcggtaagtt ttttaacgtg gtcgtaaagg tgcggcggct
    12181 cgtactcgcc gtaggctatt ttacgcagat ggaaaatcgc caaacgcgcg gcaaggtatt
    12241 ggtagtccgg ggtatcttcc gaaattaaat cggcagcggc tttgatgatg gtttcgtgga
    12301 tgtcgtcggt gcggatgccg ttgtagaact ggatgtgcga tttcaactcg acctgagata
    12361 cggaaacatt ttccaatccg tccgccgccc aagtgacgac acggtgaatc ttatccaaat
    12421 caatggcttc taatcttccg tctcgtttgg ttactttcaa atcagtcggt gtattcatcg
    12481 cttcctcttc cactcttgat attcaagaca cagtcttttc aaataaatta aggcagacaa
    12541 tatagtggat ttttggcatt ttctccagtc ttgacaacgg ttgtattttt cagtttcagg
    12601 caatgcgcga taaaacccgc ctgtcgtatt tttcctataa tatttgtttt atctgataat
    12661 tctttaccga taaaaaacgg gtaaattttt tgccttttga ccggctccgg ctacaaggcg
    12721 gcgaaataag gattttccga cgaaaaagga aagcttcctg ttttctgccc ctgcaaattg
    12781 ttaaattttg caaagtatga ttttgccgcg ccgccgccga caaattccat tttcttaccg
    12841 attggaattt attattgaga ttaatgtgtt atttgaatcc gcatatcaaa cggcaagttt
    12901 tgtcggctga atgagtctga aactgccgac aatttgcccg ttccatcctt ccatcctata
    12961 ctggaaggaa tgacaaactg aaaggatcgt catatctgtc aaaaagatgc ttgccatact
    13021 gttgtctgca atattgggac tggtatcgac aactgccgct gccggtacgt cagaacccgc
    13081 ccaccgcgat accaaacata tccgcaaggc aaacaagcag atgctgcacc ccgaatgcag
    13141 gaaatatttg gaacgccgtg ccgcgtggta ccgatcgcaa ggcaacgtgc aggaattgcg
    13201 cgaaaacaaa aaggcgcgca aagcattccg ctccctgcct tataaggaac agaaaaccca
    13261 atgccgggcg gcttatgagg ctttcgatga tttcgacggc agcaggttcc gccgttaatc
    13321 ccatataaaa tatgccgtct gaacacaggt tcagacggca tattttatga atacaacaaa
    13381 ctatccttac gccatttcgt ttgaggtcct cccgttgccg cagccttaat atagtggatt
    13441 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aga
//
LOCUS       DAWKOD010000043        13416 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000043 DAWKOD010000000
VERSION     DAWKOD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.043"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(121..1185)
                     /locus_tag="WJK73_000930"
     CDS             complement(121..1185)
                     /locus_tag="WJK73_000930"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ7557429.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            1379..2296
                     /gene="xerC"
                     /locus_tag="WJK73_000931"
     CDS             1379..2296
                     /gene="xerC"
                     /locus_tag="WJK73_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ7557430.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     AADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            2381..4294
                     /gene="dxs"
                     /locus_tag="WJK73_000932"
     CDS             2381..4294
                     /gene="dxs"
                     /locus_tag="WJK73_000932"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ7557431.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(4379..5707)
                     /gene="miaB"
                     /locus_tag="WJK73_000933"
     CDS             complement(4379..5707)
                     /gene="miaB"
                     /locus_tag="WJK73_000933"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ7557432.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            5910..6323
                     /locus_tag="WJK73_000934"
     CDS             5910..6323
                     /locus_tag="WJK73_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7557433.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLRDQTEEKIVQFAVRGKTT
                     RAEVEARFGRQNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDGEGVVRD
                     YRFTHKVEKDERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            6387..7670
                     /gene="hemL"
                     /locus_tag="WJK73_000935"
     CDS             6387..7670
                     /gene="hemL"
                     /locus_tag="WJK73_000935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ7557434.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(8167..8730)
                     /gene="orn"
                     /locus_tag="WJK73_000936"
     CDS             complement(8167..8730)
                     /gene="orn"
                     /locus_tag="WJK73_000936"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ7557435.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(8748..9635)
                     /gene="prmA"
                     /locus_tag="WJK73_000937"
     CDS             complement(8748..9635)
                     /gene="prmA"
                     /locus_tag="WJK73_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ7557436.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(9753..11114)
                     /gene="accC"
                     /locus_tag="WJK73_000938"
     CDS             complement(9753..11114)
                     /gene="accC"
                     /locus_tag="WJK73_000938"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ7557437.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSMQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(11227..11652)
                     /locus_tag="WJK73_000939"
     CDS             complement(11227..11652)
                     /locus_tag="WJK73_000939"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ7557438.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQVKAGDTLCI
                     IEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(11634..11891)
                     /locus_tag="WJK73_000940"
     CDS             complement(11634..11891)
                     /locus_tag="WJK73_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ7557439.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            12008..13048
                     /gene="queA"
                     /locus_tag="WJK73_000941"
     CDS             12008..13048
                     /gene="queA"
                     /locus_tag="WJK73_000941"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ7557440.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         2792 a         3427 c         4045 g         3152 t
ORIGIN      
        1 taaacatttt ggttatggta tctttttttg catactgcat caatgaggca ggtcaaagaa
       61 gcaaaaatca aatgccgtcc gaacaacggt tcagacggca ttttgtttac aagcaacctg
      121 ttatttgacg atttggttca attcgccctt ggcataacgg tttgccattt tttccaagga
      181 aaccggtttg attttgcctg cctgaccttc gcaaccgaac gcgaggtagc ggtcgaggca
      241 gatttgcttc atcgcttcaa tggttttgct caaatatttg cgcggatcga agtcggacgg
      301 gttttcggca aggtagcggc gtaccgcgcc ggtggaagca aggcgcaagt cggtatcgat
      361 gttgacttta cgcacgccgt gtttgatgcc ttcgacgatt tcttcaaccg gcacgccgta
      421 ggtttcgccg atattgccgc cgtattcgtt gatgactttc agccattctt gcggaacgga
      481 gctggagccg tgcatcacga tgtgtgtatt gggcagggct tggtggattt ctttgatgcg
      541 gtcgatacgc aacacgtcgc ctgtgggcgg acgggtgaat ttgtacgcgc cgtggctggt
      601 gccgacggca atcgccaatg cgtcaacgcc ggtatcttta acgaaacgca cggcatcttc
      661 gacgctggtg agcatttggt cgtgggaaag tttgcccact gcgcctacac cgtcttcttc
      721 gccggcttcg ccagtttcga ggttgcccaa tacgccgatt tcgccttcaa cggatacgcc
      781 gcaggcgtgg gagaaattaa ccacggtacg ggtggcgttg acgttgtatt cataagaaga
      841 aggggttttg ccgtcttcca tcagcgagcc gtccatcatc acggaggaaa agcccagttg
      901 gatggagcgt tggcacacgt cgggcgatgc gccgtggtct tggtgcatca cgacggggat
      961 gtgcggaaat tcttcgacag ccgccaaaat caggtggcgc aaaaacggcg cgcccgcgta
     1021 tttgcgcgca cctgcgctcg cctgtacgat gacgggcgcg ttgacttggt cggcggcttc
     1081 cataatggcg cgcatttgtt cgaggttgtt gacgttgaac gcgggcaggc cgtagctgtt
     1141 ttcggcagca tgatcaagca gttggcgcat ggatacgagt gccatttgtg tctccttggg
     1201 caataggtaa ataaggcgga ttataatgtt ttttgcggca aaaaaccata aacggctcat
     1261 tgattttatg ttaacgataa cggcgggcgg acgggtcgga ttttgggcgg ttcggggttt
     1321 tggtgtgtcg tttatgataa tgttttcact gtttttttga tatttgtgtg gggcggttat
     1381 ggttttggac gggtttgcgg cgcattttga cgcttatttg gaaaacatcg tgcgcgaggg
     1441 caagtcggag cacacagtgg cggcataccg gcgcgatttg gaagaactgt ttgcgctgtt
     1501 ggcacaaatg ccgtctgaag ctgaaggcgg cgtgccgcag gacttgtcgc ggcgcgattt
     1561 tacggcggcg ttgcggcggc tgtcgcagcg cggtttgaat gcgcggacgc tggcgcgcaa
     1621 gctgtcgtct tggcggcagt attgtgtttg gctggtggaa cgcggtctgc tgcataccga
     1681 cccggccgcc gacatcaaac cgccgaagca gcccgagcgc gtacccaaag ccctgccgca
     1741 ggaatggctg aaccggatgc tggatttgcc cgtggacggc ggcgacccgc tggcggtgcg
     1801 cgaccacgct ctgttcgagc tgatgtacgg cagcggtttg cgcgtgagcg agatacacgg
     1861 cttgaatgca gatgatgtct atttggacga ggcgtgggta cacgttaccg gcaaagggcg
     1921 caagcagcgg caggtgccgc tgaccggcaa aagcgtggaa gccctgaaaa actatctgcc
     1981 gctgcgtcag acggcatcgg acggcaaagc cctgtttacc ggcaggaacg gcacgcgcct
     2041 gagccaacgc caaatccaaa aacgtttggc gcaatgggcg gcgcaaaacg gcgacggcag
     2101 gcacgtttcg ccgcatatga tgcgccacag ctacgccgga cacctgttgc aggcttcgcg
     2161 cgacatcagg gcggtgcagg agctgctcgg gcacagcagc ctttcgacca cgcagattta
     2221 taccaagctc gatttcgacc acatcgcccg cctgtatgac gaagcccacc cgcgcgccaa
     2281 gcggcaggac gaatgacata cggcaaaatc agccgtcagc cgcacgctct tgatatataa
     2341 ttggccgttg cacccggacg acacataaaa aagacacacc atgaacccaa gccccctact
     2401 cgacctgatt gacagcccgc aagatttgcg ccgtctggac aaaaaacagc tgccgcgcct
     2461 tgccggcgag ttgcgcgcct ttctgctgga atctgtcggg cagaccggcg ggcatttcgc
     2521 cagcaatttg ggcgcggtcg aactgaccat cgccctgcac tatgtgtacg atacgcccga
     2581 agacaagctg gtatgggatg tcggacacca aagctatccg cacaaaatcc tgacaggcag
     2641 gaaaaaccag atgcacacca tgcgccaata cggcggttta gctggcttcc caaaacgcag
     2701 cgagtcggaa tacgacgcgt tcggcgtagg gcattcttcc acatccatcg gcgcggcgtt
     2761 gggcatggcg gcggcagaca aactcttggg cagcgaccgc cgcagcgtcg ccatcatcgg
     2821 cgacggcgcg atgacggcgg ggcaggcgtt tgaagccttg aattgcgcag gcgatatgga
     2881 tgtggatttg ctggtcgtcc tcaacgacaa cgaaatgtcg atttccccca acgtcggcgc
     2941 gctgccgaaa taccttgcca gcaacgtcgt gcgcgatatg cacggcctgt tgagtaccgt
     3001 caaagcgcaa acgggcaagg tattagacaa aatacccggc gcgatggagt ttgcccaaaa
     3061 agtcgaacac aaaatcaaaa cccttgccga agaagccgaa cacgccaaac agtcgctgtc
     3121 tttgtttgaa aacttcggct tccgctatac cggccccgtg gacggacaca acgtcgaaaa
     3181 tctggtggac gtattgaaag acttgcgcag ccgcaaaggc cctcagcttc tgcacgtcat
     3241 caccaaaaaa ggcaacggct acaaactcgc cgaaaacgac cccgtcaaat accacgccgt
     3301 cgccaagctg cctaaagaag gcgcggcgca aatgccgtct gaaaaagaac ccaagcccgt
     3361 cgccaaaccg acctataccc aagtgttcgg caaatggctg tgcgaccggg cggcggcaga
     3421 cgagcgtttg atcgccatca cgcccgcgat gcgcgagggc agcggactgg tggagtttga
     3481 acaacgattc cccgaccgtt atttcgatgt cggtattgcc gagcagcacg ccgttacctt
     3541 tgccggcggt ttggcgtgcg aaggcatgaa gcccgtcgtg gcgatttatt ccaccttttt
     3601 acaacgcgcc tacgaccaac tggtgcacga tatcgccctg caaaacctac ccgttttgtt
     3661 tgccgtcgac cgcgcgggca tcgtcggcgc ggacggcccg acccacgccg gtttgtacga
     3721 tttgagcttc ttgcgttgca ttccgaatat gattgtcgcc gcgccgagcg atgaaaacga
     3781 atgccgcctg ctgctttcga cctgctatca ggcggatgcg cccgccgccg tccgctatcc
     3841 gcgcggcacg ggtacgggcg cgccggtttc agacggcatg gaaaccgtgg aaatcggcaa
     3901 gggcattatc cgccgcgaag gtgggaaaac cgcattcatt gccttcggca gtatggtcgc
     3961 ccccgcattg gcggtcgccg gaaaactgaa cgccaccgtc gccgatatgc gctttgtcaa
     4021 accgatagac gaagagttga ttgtccgcct tgcccgaagc cacgaccgca tcgttaccct
     4081 tgaagaaaac gccgaacagg gcggcgcagg cggcgcggtg ctggaagtat tggcgaaaca
     4141 cggcatctgc aaacccgttt tgcttttggg cgttgccgat accgtaaccg gacacggcga
     4201 tccgaaaaaa cttttagacg atttgggctt gagtgccgaa gcggtggaac ggcgtgtgcg
     4261 cgcgtggctg tcggatcggg atgcggcaaa ttaagccgcc cggccgcgcc gtcgtctgcc
     4321 gatacaaggc aaaatgccgt ctgaaacgct tcagacggca ttttttcggc gtgagggttt
     4381 aggcttcgac aactttgccg cgcagggaga aggtgtaggc ctcggtgatt tccaaatcga
     4441 tcatttggtt aatcatgtcg ggcgtgccgg tgaagttgac gacgcggttg ttggcggtac
     4501 gggcttgcag ttggtcgggg tcttttttgg agatgccttc gaccaggcag cgttgaaccg
     4561 tgccgatcat ggtttggttg atgcgcgcgg tttcggcttc gatgacttcg ttcaaggctt
     4621 cgaggcggcg cactttttct tcgtgcggcg tgtcgtcgtg caggttggcg gcaggcgtgc
     4681 cggggcgcgg actgtaaata aacacgaagc tcaagtcgaa ggcaatgtct ttcaccagtt
     4741 tcaaggtttg ctcgaactcg cgttcggtct cgccggggaa gccgacgatg aaatcgctgc
     4801 tcaggcataa atcaggacgg atggcacgca gtttgcggat gatggatttg tattccaaag
     4861 cggtgtagcc gcgtttcatc gcgctcaata cgcggtcgga accgctttga atcggcaggt
     4921 gcaggtggga aaccaatttg ggcaggtcgc ggtagcactc gataatcgag tcggtaaact
     4981 cgcgcgggtg gctggtggtg aagcgcatac gttcgatgcc ggggatttcg tggacgatgc
     5041 gcagcagggt ggcgaagtcg cagatttcgc cgtcgtccat ttcgccgcga taggcgttga
     5101 cgttttgtcc caagaggttg atttctttca cgccttgctg ggcaaggttg gcgatttctg
     5161 tcaatacgtc gttgagcggg cgggagaatt cttcgccgcg cgtgtagggg acgacgcaga
     5221 atgagcagta tttggaacag ccttccataa tcgatacaaa tgccgcgccg ccttcgacgc
     5281 gggcgggcgg caggtggtcg aatttttcga tttcggggaa ggaaatatcg acttgcgaca
     5341 gcccgctggt ttctttgtcc acaatcattt tgggcaggcg gtgcagcgtt tgcgggccga
     5401 agaccacgtc aacataaggc gcgcgtttga tgatgttttc gccttcttgc gaggcgacgc
     5461 agccggcaac gccgatgatg aggccggggt ttttttcttt gagcggacgc acgcgcccca
     5521 aatcggagaa gactttttcc tgtgcttttt cgcgcacgga gcaggtgttg aacaagatga
     5581 tgtcggcttc gtcggcttgg gtaacctgtt cgatgccgcc gtgttcttcg gcgaggacgg
     5641 cgagcatttt gtcgctgtcg tattcgttca tctggcagcc gaaggtgcgg ataaatactt
     5701 ttttcatggt ttgtgtcttt ctcaggcagc cgtaatcgcg gggctgattg ttgttggaat
     5761 gaaaaaattt cagacggcat gacgatgccg tctgaaaatc ggtgcggatt atagcacgat
     5821 gtgggttttg taaacagaat attgttttaa aatatgaatt taatcggtcg ggacggctgt
     5881 ataatgtttg gctttaatgg gaggtgtgta tgaaaccggc tgtgtgggcg gcattgctgc
     5941 tgtgtgcgtg taccagcaat ttcggcgaca gggaacatca gttcctgcgt gatcagacgg
     6001 aagaaaaaat cgtgcagttt gcggttaggg gcaagacgac ccgggcggaa gtggaagcgc
     6061 gtttcgggcg gcaaaatccg ttcggctgtt atgcctatca cgaggtcagc ctgcctattt
     6121 ataatttttt gccgaccaat ttcatttata tgaaatcaga acgccggcat tgggagtggt
     6181 gtgtggatta cgacggggag ggagttgtcc gggactaccg ctttacacat aaagtggaaa
     6241 aagacgagcg ttccgtcatc cgggatgcgg ttggcgtcat ccgcaaagaa gcaggcaaat
     6301 ccttgtcgca acctgaaaaa tgataaaatg ggactttctg cttctaagcc cgaaacctgc
     6361 cgttcaggcg gcatttgagg ataaatatga accgtaacga aattttattc gaccgcgcca
     6421 aagccatcat ccccggcggc gtgaattccc ccgtccgcgc attcggcagc gtcggcggcg
     6481 tgccgcgctt catcaaaaaa gccgaaggcg cgtatgtttg ggacgaaaac ggcacgcgtt
     6541 ataccgacta tgtcggctct tgggggcctg cgattgtcgg acacgcgcac cccgaagtcg
     6601 tcgaagccgt gcgcgaagcc gcgttgggcg gtttgtcgtt cggcgcgccc accgaaggcg
     6661 aaatcgccat tgccgaacaa atcgccaaaa tcatgccgtc cgtcgaacgg ctgcgccttg
     6721 tcagctccgg taccgaagcc actatgaccg ccatccgtct ggcacgcggt tttaccggcc
     6781 gcgacaaaat catcaaattt gaaggctgct accacggcca ttccgacagc ctgttggtga
     6841 aagccggcag cggtctgctg acgttcggca acccgtcttc cgccggcgtg cctgccgact
     6901 ttaccaaaca cactttagtc ctcgagtaca acaatatcgc ccaactggaa gaagcctttg
     6961 ctcaaagcgg caatgacatt gcctgtgtga ttttggagcc ttttgtcggc aatatgaatc
     7021 tggttaggcc gtctgaagcc tttgtcaaag ccttgcgcga attgaccgaa aaacacggcg
     7081 cggtgttgat ttacgacgaa gtgatgaccg gtttccgcgt cgcgctcggc ggcgcacaat
     7141 cgctgcacgg catcacgccc gacctgacca cgatgggcaa agtcatcggc ggcggtatgc
     7201 cgcttgccgc gttcggcggg cgcaaagaca ttatggaatg tatttcccca ctgggcggcg
     7261 tgtatcaggc aggtacatta tcaggcaacc cgattgccgt cgccgccggt ttgaaaacgc
     7321 tggaaatcat ccgccgcgaa gggttttatg aaaacctgac ggcgcgtacc gaacagttgg
     7381 ttcaaggttt tcagacggca gcggatgcgg cgggcatcga gttcgccgcc gacagcgtgg
     7441 gcgggatgtt cggcctgtat tttgccagcc atacgccgcg aaactatgcc gatatggcgc
     7501 gatccaatat cgacgctttc aaacgcttct tccacggcat gctcgaccgc ggcattgcct
     7561 ttggcccgtc cgcttatgaa gcaggtttcg tttctgccgc gcatacgccc gagctgattg
     7621 acgaaacggt tgcggttgcg gttgaagtgt tcaaggcgat ggctgcatga tgttttgacg
     7681 gacagagttt ctctgttcga tttgtttggc agattgaagt aagaatgcac accgccgtca
     7741 ttcccgcgca ggcgggaatc cagacctttc agtttctgta atgattgaaa ataacgggcc
     7801 gaaatcagcc gcgcccggca ggaagccgcc gccacctgaa acaatgccgt ctgaagcctt
     7861 cagacggcat tttatcggaa gcgcatcaga aaaaacggat agcgacatat gcggtaaaag
     7921 caagggcaat tacatagata aaccccttta taaattcaaa agtctcatct atatcgctat
     7981 atttttcttt cagttcgtca gattttcgat aacgtttcat tttcaagacc acttcatgac
     8041 caacaagccc gaccttccgg attcccgcct gcgcgggaat gacgggcgtg tatatttttg
     8101 atttcaatct gctgtaaaaa tgccgtctga aatgcaaaca gcgtttcaga cggcattgtt
     8161 tgtttcttat tgcgcttcgg ctctcggggc ggaaatcaga aagtgttcgc ggtagtggcg
     8221 catttcttcg atgctctcca aaatgtcgtc caatgccttg tgcgaaccgc gtttgacgac
     8281 gcttttggca acgggcggat tccagcgttt ggcaagctct ttcagtgtgg aaacgtcaag
     8341 gttgcggtag tggaagtagt tttccagttt cggcatatat ttgaccataa aacgccggtc
     8401 ttggtggatg gaattgccgc acatcggcgt ggcgcgtccg ggtacccatt ccgacataaa
     8461 gtccagcagt ttctgttcga cttcggcttc ggtatgcgac gattcgcgta cgcgctgtgt
     8521 cagccccgtc ctgccgtgtg tggcggtgtt ccactcgtcc atattgtcga gcagctcgtc
     8581 actttggtgg acggcgtaaa cttcggattg cgccaataca ttcaaatccg agtcggtaat
     8641 aatcatcgcg acttcgataa tgcggtcggt ttcgggatta agccccgtca tttccatatc
     8701 gagccagcag aggttgtttt tgtcctgcac ggtgtttcct ttccgtttca gcgttttgtc
     8761 ccgctcaagc gcgcccagct ttcttcggtt tccgccgggt cgagatcgaa ccattggctg
     8821 taaatgccgc cgagttcttc ggcttgttcg tccaacaaac cggataacac gatgcggccg
     8881 ccttgtttgg tgcgggcggc gagcatttcg ccgagcatac gtaaagggtt ggcgaggatg
     8941 ttggcgacaa ctacgtcgaa ttgcccttga ggcagaccgt cgggcaggaa gaattgtgca
     9001 tcgacgttgt tctgttcggc gttgtccttg ccggcgcgga cggcttgttc gtcaatatcc
     9061 acgccgacgg cagaacctgc gccgagtttg agggcggcga tggtcaggat gcccgaaccg
     9121 cagccgtagt cgaggacgct ttcgccgttt ttgagttgcg tatccaacca tttgaggcag
     9181 aggcgcgtgg tcgggtggct gccggtgccg aaggcgagtc cggggtcgag gcggaggttg
     9241 acggcgctgc cttcggggac ttcgtgccaa gagggggtaa tccacaggcg gtcggaaatc
     9301 cggatggggt cgaattgcga ttgcgtgaga cgcacccagt cttggtcttc gatggtttcg
     9361 ccggtgtatg ccaagtcttt taacccgcat tcttgtgcgg cgatttggat gatggcggcg
     9421 gcttcgtcgt gttcgccgaa cagggcgatg actttgctct gctgccagat ttgttcggcg
     9481 ggcataccgg gttcgccgaa aatcgcctgt tcgttttgcg tgccggcgta ggcatcttcg
     9541 atggcggcgg agagtgcgcc gtgttccatc agcgcgtcgg cgaggcgttc ggcgacggca
     9601 tcgttgacgt tgacggtgat ttgttggtag ggcatagcgg gctttcttaa cagaccttcg
     9661 gaatagggtc gtactgtata aggggatggg tgtgtaacgg aaatgccgtc tgaaaacgga
     9721 caaactgttt cagacggcat atcggggacg gcttatttgt cctgtttggc tttgcgatct
     9781 tccagccagt gttccaaata gtggatgctg acgccgcctt tttggaaacc cgcatcggcg
     9841 aacaggtcgc ggtgaagcgg cgtattggtt ttgatgccgg ttaccgccag ctcggcgagt
     9901 gcgacgcgca ttttcgccat tgcctgttca cgcgttttgc cgtgtacgca gattttgccg
     9961 atcaggctgt cgtagtacgg tgggatgcgg tagccttggt aaatgtggct gtccacgcgg
    10021 ataccgaagc caccgggcag gtggcagctt tcaatcgggc ccgggctggg gatgaagttg
    10081 tacgggtctt cggcgttgat acggcactca aacgcgtggc cttcgacttg aatatccttt
    10141 tgtttgtatt gcaaaggcag gccggcggcg atgcggagtt gctcctgcac gatgtccacg
    10201 ccggtaatca gctcggtaac cggatgctca acctgaacgc gcgtattcat ctcgataaag
    10261 aaaaattcgc cgtcttcgta caggaattca aacgtacctg cgccacgata gccgatgcgt
    10321 ttgcaggcgt cggcacaggc gttgccgatt ttggcgcgtt ctttttcagt gatgaacgga
    10381 gccggtgctt cctcgatgac tttttggtgg cggcgctgca tggagcagtc gcgctcggcg
    10441 aggtagatgg cgttgccgtg ttcgtcggca atcacttgga tttcgacgtg gcgcggacgc
    10501 tgcaggtagc gctccatgta aaccatcggg ttgccgaatg ccgcgcctgc ttcggctttg
    10561 gtcatttcga cagactggag gaggtcttct tttttctcga caacgcgcat accgcgaccg
    10621 ccgccgccac cggaggcttt gataatgacg ggataaccga ctttatcggc gattttgagg
    10681 atttcttcgc cgtcgtccgg caatgcgccg tcagaaccgg ggacgcaggg tacgcctgcc
    10741 gctatcatcg cgtgtttggc ggagactttg tcgcccatca ggcggatggt gtcgggtttc
    10801 gggccgataa aggtaaagcc ggactgttcg acctgttcgg cgaaatcggc gttttcggca
    10861 aggaaaccgt agcccgggtg gacagcgtcc gcgcagctta cttcggcggc ggcgataatg
    10921 gcggggacgt taaggtagct ttgcgcggaa gcggcagggc cgatgcacac ggattcgtcg
    10981 gcgagtttga cgtgcaggct gtctttgtcg gcctcggaat gcacggcgac ggtggcaatg
    11041 cccatttcgc ggcaggcacg gagtacgcgt aatgcgattt cgcctcggtt ggcgattaaa
    11101 acttttttca gcatgatgac tgttcctgtg attccggcaa cccgtccgta aaaaaaaggt
    11161 gcgggaacgc cgaaggggga aagcatttca gacggcatcg gaagtttatg ccgtctgaaa
    11221 acaggattat ccgatgatga agagcggttc gccgaattcg acgggcgtac cgttttcgac
    11281 cagaatttct ttgaccgtgc cggatttttc ggcttcgatt tcgttcatca gcttcatcgc
    11341 ttcgatgatg cacagcgtgt cgccggcttt aacctgttgg ccgacttcga caaaaggcgc
    11401 ggcattcggg ccgggtgcgc ggtagaatgt gccgaccata ggcgattttt gcgcgtcgga
    11461 caaatcgcgg gcggcaggtg cggcggcagg cgttacggcc ggcgcggcgg caggtacggg
    11521 cgcggcgtaa acgggtgcgg cggcgatggt tcgggtgatg cggacttttt cctcgccttc
    11581 ggttacttcg atttcggcga tacccgattc ttcaaccaaa tcaatcagtt tttttaattt
    11641 gcgcaaatcc atttctgttc ctttaaaggc tgccggtgtt ccggcgggtt gttgcgttgg
    11701 tttttcagaa aacagccggc ttgcggcaac ggcgcaaacc gggcggaatg gtgttcggat
    11761 acggttttcc tgtggtggaa aacaaccgtg agaatgtgtt tattttcccg aagttgtcgg
    11821 taaatgtcca gtaaaatatc aaaaaacggc ggtttttggg ggaaatgtgc aggaagcttg
    11881 attttgcgca caagatgccc cgggtcaaga gtgtttattc taatctgttg gtttttcggg
    11941 caaagatgcc gtctgaaaaa gggctaaagt gcgtataatg gcggcttgcc cgaacgagag
    12001 tgtaaaaatg gatatttcag attttgattt taccctgccc gaaaagctga ttgcccagca
    12061 tccgcccgaa gtgcgcggca gcagccgcct tttggttgcg ttgcccgata tgccgctgca
    12121 agaccgggtg ttcggcgatt tgccggatta tgtcgaggcg ggcgacgttt tggtgttcaa
    12181 caacaccaaa gtcatgaagg cgcggctgtt tgggcagaaa gacagcggcg gcaggatcga
    12241 agccctgatt gagcgcgtgt tggacaacca taccgcactg gcgcacatcc gttcgtccaa
    12301 gtcccccaag cccggtatgg ggctggtgtt tgaaggcggc atccgtgccg tgatggtcgg
    12361 gcgtgagggc gaactgttct gcctgcgttt tgaaggcggt caaaccgttt acgaactttt
    12421 ggaacagaac ggccatttgc ctctgccgcc ttatatcgaa cgtgccgccg acgcggacga
    12481 cgacagccgt tatcaaaccg tttatgccaa atatcagggt gcggtcgctg cgccgacggc
    12541 gggcctgcat tttacggaag aacttttgca ccgtctgaaa gacaaaggcg cggtaaccgc
    12601 agaagtaact ctgcacgtcg gtgcggggac attccaaccc gtgcgtgtgg acaaaatcga
    12661 agagcataaa atgcacagcg aatggtttga agtgccgtct gaaaccgccg ccgccgttga
    12721 ggcggcaaaa gcccggggga acaaagtctg ggcggtcggc acgacttcca tgcgcgccct
    12781 cgaatctgcc gcgcgtgcga cgggtcgtct gaaagcgggg cagggcgaca ccgatatttt
    12841 catcacgccg ggctaccgtt ttaatgttgt cgacaggctg gttaccaatt ttcatttgcc
    12901 gaaatcgacg ctgctgatgc ttgtcagcgc gttttcgggt atgggtcata tccgcgccgc
    12961 gtatcgttat gcggtcgaac gcgaataccg tttcttcagc tacggcgatg cgatggtttt
    13021 ggggcggaac gaaggggtag tacgttagaa cagccccaag tctattgggg gaagacaagt
    13081 gccgtctgaa cggacgttcg gacggcattc ttttgttgat ttgaatgaaa ggaactgtat
    13141 gacatagatt caggcgcgag aggcgagaaa ctcagtcatt gttgatgacg cgcaatacgt
    13201 taaaattcat tccgttttgg tcgtataaac atattacata cgccattttt ttgtattcaa
    13261 ggctgcctga aaacagtggg caagaatatc taacccaacg aaaagctgaa aaagcaacaa
    13321 aaaataatct gcaccttagt cggttggtta gtccaacctt aggggtgcag accattcaat
    13381 ctttcaggct gcctttgttt gtttataaga taaatt
//
LOCUS       DAWKOD010000044        13387 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000044 DAWKOD010000000
VERSION     DAWKOD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.044"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..538)
                     /locus_tag="WJK73_000942"
     CDS             complement(110..538)
                     /locus_tag="WJK73_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ7557441.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WJK73_000943"
     CDS             complement(603..2351)
                     /locus_tag="WJK73_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ7557442.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDCFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WJK73_000944"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WJK73_000944"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ7557443.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WJK73_000945"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WJK73_000945"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ7557444.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WJK73_000946"
     CDS             4754..4924
                     /locus_tag="WJK73_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ7557445.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WJK73_000947"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WJK73_000947"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ7557446.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WJK73_000948"
     CDS             6085..6279
                     /locus_tag="WJK73_000948"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7557447.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WJK73_000949"
     CDS             6481..7890
                     /locus_tag="WJK73_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7557448.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7991..11200
                     /gene="recC"
                     /locus_tag="WJK73_000950"
     CDS             7991..11200
                     /gene="recC"
                     /locus_tag="WJK73_000950"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ7557449.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WJK73_000951"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WJK73_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ7557450.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13172..13384
                     /locus_tag="WJK73_000952"
     CDS             13172..13384
                     /locus_tag="WJK73_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557451.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3354 a         3199 c         3559 g         3275 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgt tttttggaaa caatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaccgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat caggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag
     7261 gttaaggaag tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac
     7561 ccgttcggca aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg
     7621 agcgtgttgg cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa
     7741 atcaagctgc ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc
     7801 ccgaccgcgc tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca
     7921 ttttgtttga aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt
     7981 tgtcgaaatc atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt
     8041 tgcccgcatt caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt
     8101 gcagagtcag gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc
     8161 ggcgaatttg tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat
     8221 tcccggtatt ccggaactca gcccgtttgc acccgaagtc atgcgctggc ggctgctgga
     8281 tttgttccgc agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct
     8341 gcaagactat ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga
     8401 catattcgac caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag
     8461 gcggctcggt ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga
     8521 tggcaggcag agcgcgccgc accgtgtcgc gttgtgggaa aagctgttgg aatctttaag
     8581 cagtgataag ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta
     8641 tttgcagctt ttgcacaagc tgtccgaaca ttgcgatgtg ttcgtgtttg cactcaatcc
     8701 gagtgggatg tattggggca acgtcatcga agcggcgcaa atcctcaaag gcggcggcga
     8761 tcccgattta actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcgcga
     8821 ctttttcgac tttttgaacg aaatggaaat agaaggagaa acgccggtat ttgaagaagg
     8881 cgggcgcgat acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc
     8941 tgaaaatgtg ggaagcgtcg gtacggatga cggttcgata cgtatcgtct ccgcgcacag
     9001 ccctttgcgc gaattgcaga tactcaaaga caagctgttg aaaattctgc atgaacatcc
     9061 cgattggcag ccgcatgata tcgccgtatt aaccccgaat atcgaatcct atacgccttt
     9121 tatcgaggcc gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc
     9181 ggacgtgaaa ataagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt
     9241 gttggaaagc cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt
     9301 gcgccgtttc ggactgactg aggacgattt gccgcttttg cacgacatgg ttgccgattt
     9361 gaacgtccgc tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg
     9421 gaagcaggcg gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaatccgat
     9481 gtggcaggat gtcagcgcgt ggtatgccga cgtgaatcaa accgccatgt tcggacgttt
     9541 tgccgccttc ctcgaaaccc tgtcggatat tgtacggata tggcggcaac ctgcgacggt
     9601 tgatgaatgg gtaggacgtt gccgaggttt gcttgaaaca ttgttccaag ccgaagccga
     9661 tgaccaaaag gcagtccaaa acctcgaaaa cgaatgggtc aaatggcagg cggaaacaga
     9721 attggcgcaa ttttccggac agttgccgca acaaaccgtc atccgccata tccgacgttt
     9781 cctcgacagc gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt
     9841 gccgatgcgg agcctgccgt tcaaagtcat ctgcctgttg ggtttgaacg acggagattt
     9901 tccccgcaat accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga
     9961 ccgagcccgt cgcgatgacg accgctacct gttccttgaa gctctcatca gcgcgcgtga
    10021 aatcctgtat ttgtcctaca tcgggcgcga tatccgcaaa gacgaagagc ttgccccgtc
    10081 ttcgctgttg ggcgaactca tcgacacagt tgccgccatg accggcacaa acagccgcca
    10141 acttgcacaa aattggatag aacagcatcc gctgcaagcc ttctcgcgcc gatatttcca
    10201 agaaggcgga cgttcagacg gcatattcgg cacgcgtacc gactacgcca ctgcgctcgg
    10261 acaaacgccc gaaccgccgc aacccttttt cgaccaaccc gtagaaaacg ccgaacctgt
    10321 tgccgaaatc ggacaggacg aatttatccg tttctggcgc aaccccgtca aagtatggct
    10381 tcagcagcag cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc
    10441 cttcgagccg caacacgccg atcaaatcgc cgaaacctac atcgaagcac ggcgcgaagg
    10501 acgggatttt gcccaaaccg ccgcccgcat cggggcggaa agcctcctgc cgtcgggaga
    10561 gttgggcaga ctttggcagc agaacttcca aactgctgcc aaacaaatcg acacggcggt
    10621 tttaaacagc cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct
    10681 gaaaggcagc ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc
    10741 caacgcgccg caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc
    10801 gtctgaagcc gaaacgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc
    10861 ggaaatcgcg caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat
    10921 cgggcaaaac cgcccgctgc cgttttttgc caagacctcg cttgccgctg ccgaagcgtt
    10981 tgcccaaaaa caagattggg aagccgccct gaaaaaagcc caaaccgcct attacggcaa
    11041 caaagtcagc aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag
    11101 ccaaaaccca ctcgaacaac ccctgtttga aaacctcgcc cgcctgcttg ccgacacgct
    11161 tgccgcagcg gaaaaaaggg aaggggccgg agcagcctga cgggatggaa aggcaaatgc
    11221 cgtctgaaaa cagcaaaaag gttttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatacaaagt gggtgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccatgcaatt aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcactgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ttgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagcgca aacaacaaga
    11881 aaataaaaac aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggcgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagtccgaa atacgcaccg gcggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt
    12901 ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat
    12961 gcccacatcg ttattctcat aaaggcggta atccgaaaac ttgacgttaa agccttatca
    13021 ggaatgactg gaactcaaca aagcggtttc ccgctttcgc ggaaatggcg ggattttcgg
    13081 tttttgtttg caaaaatgcc ggagaaaaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaattatgga
    13201 caaatatgat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgttgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat
    13381 ttagtga
//
LOCUS       DAWKOD010000045        13250 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000045 DAWKOD010000000
VERSION     DAWKOD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.045"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..2314
                     /locus_tag="WJK73_000953"
                     /pseudo
     CDS             168..2314
                     /locus_tag="WJK73_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2304..5084
                     /locus_tag="WJK73_000954"
     CDS             2304..5084
                     /locus_tag="WJK73_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7557452.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(5228..6400)
                     /locus_tag="WJK73_000955"
     CDS             complement(5228..6400)
                     /locus_tag="WJK73_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ7557453.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6690..7136
                     /gene="iscR"
                     /locus_tag="WJK73_000956"
     CDS             6690..7136
                     /gene="iscR"
                     /locus_tag="WJK73_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ7557454.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7165..8379
                     /locus_tag="WJK73_000957"
     CDS             7165..8379
                     /locus_tag="WJK73_000957"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ7557455.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            8640..9026
                     /gene="iscU"
                     /locus_tag="WJK73_000958"
     CDS             8640..9026
                     /gene="iscU"
                     /locus_tag="WJK73_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ7557456.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9113..9433
                     /gene="iscA"
                     /locus_tag="WJK73_000959"
     CDS             9113..9433
                     /gene="iscA"
                     /locus_tag="WJK73_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ7557457.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9393..9614)
                     /locus_tag="WJK73_000960"
     CDS             complement(9393..9614)
                     /locus_tag="WJK73_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ7557458.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRRE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            9696..10196
                     /gene="hscB"
                     /locus_tag="WJK73_000961"
     CDS             9696..10196
                     /gene="hscB"
                     /locus_tag="WJK73_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ7557459.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            10294..13044
                     /gene="gyrA"
                     /locus_tag="WJK73_000962"
     CDS             10294..13044
                     /gene="gyrA"
                     /locus_tag="WJK73_000962"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ7557460.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
BASE COUNT         4049 a         3255 c         2910 g         3036 t
ORIGIN      
        1 gcaaaatcac gacacactcg gacacccgcc ccgcttatcg caactttgcg aacgcccccg
       61 gaaacagcaa agacatcaaa taattgattt tattagaatc tatttgcaaa gccatttgcc
      121 gttacacaag aatggcacat taaaataact gatgaggatt tataacgatg aagacagaca
      181 ttcaaaccga attaacccaa gccctactac cacacgaaaa agtatgggcg aacgaagaaa
      241 aaaccatttt agccaaaaac atcctgttgg atttggtgga aaaaaccgac ccgaccatta
      301 tcggtttgtt attgagtaat gatgagttaa aacgccattt ctttgtggaa gtgaatggtg
      361 tgctggtgtt taaattgcag gatttccgtt ttttcttgga caaacacagc gtcaataatt
      421 cctacacaaa atacgccaac cgcattggtt tgacggacag caaccgcttt ttgaaagaca
      481 gcagtgatat tgtgttggat tttccgttta aagattgtgt gttaaatggc ggacaaagca
      541 ccgaggaagg cgaagaaatt tattttaaac gcaataacag ccagccagcc aattatacac
      601 caaattaacc cgaaaaagac aagaaatctt ttttaatcaa acgcttgctt ttgatgaaat
      661 tgaccgactt tttgacgcaa aagcattctc aaaattctct cgctataccg cagacggcaa
      721 acaagcggtt ggcgaaatca aacgccattc agacggcaca ccggccgaaa atcttattat
      781 caaaggcaat aatctgattg ccctgcattc gcttgccaag cagtttaaag gcaaagtgaa
      841 gctgatttat attgatccgc cttattattt tgttaaaaag aaacaacaag atagttttgg
      901 ttataacact aactttaaat tatctacttg gcttacattt atgaaaaatc gtttatcaat
      961 tgccaaagaa ttattaactg atgatgggat tattgttatt tccattgatg atgacggaaa
     1021 tgcttattta aagattttgt tagatgaaat ttttggattt gaaaatttta tcggaaattt
     1081 acccactatt atgaatttaa aaggtaataa tgatgaatat gcttttgcag gcactcacga
     1141 atacacacta gtctttgcta aaaataaaga taaatctact ttttatgaat ttccaataga
     1201 tgaagataat tttttagaga aatgggaaga agatgaaatt gggttttata aaaaaggagc
     1261 tccaatgcga gcaacaggaa ccgaagataa aagagaagat agacctgaaa tgttctatcc
     1321 ttttttggta aaaaataaca cagttagtac aattacagat gaagaatttt ctcaaatcta
     1381 taataaagat ttagaagtat tcaatgatga ttttattcaa aaactaaaag agaaatacga
     1441 aaatcttgga tacaatttta ttttacctat tgctaataaa caatgggggc gttggcgttg
     1501 ggggtattca ataaagaata aggcaaggct gcagacggat attattgtta gccaatctaa
     1561 aaatggaatt tccttgtata aaaaacaaag acctgaatta gatgatttac ctacaaaaaa
     1621 acctaaaact attttttata aacctgaata cagtagtggt aatggaactg aacagatgaa
     1681 aaatcttttt ggtgaaaagg catttaaaaa tcctaaacca gaagaactta tccaagattt
     1741 tattaccatc acaaccaacg aaaacgacat cgtcctagac taccatttag gcagtggcac
     1801 aaccgccgcc gttgcccaca aaatgaaccg ccaatatatc ggtattgaac aaatggatta
     1861 tattgaaacg cttgccgttg aacgcttgaa aaaagtgatt gatggcgagc aaggcggtat
     1921 ttccaaagcc gtgaattggc aaggtggtgg cgagtttgtt tatgccgaac ttgccccatt
     1981 taacgaaacc gcaaaacaac aaattttggc ttgcgaagat tcagacggca tcaaaacgct
     2041 gtttgaaggt ttatgcgaac gctatttctt gaaatacaac gtcagcgtaa atgaatttag
     2101 tcaaatcatt caagagcctg aatttcaatc cttggcatta gacgaacaaa aacaaatggt
     2161 gcttgaaatg ttggatttaa atcaaatgta tgtttcatta tccgaaatgg atgatgagca
     2221 atttgcaggt tgcttaaatg atgacgataa agcgttaagc cgtgaattct atcaatcagc
     2281 aaaaaatcaa gcggagaaaa aagatggcga ataataaaac gttatttgat tgggtggaag
     2341 accgcaaatc aacgctggaa gaaatagaac agacagattt tttcgcgctg cctgaatttg
     2401 tagaacgcaa tttaaaatat ccgttttttg aatggcaaaa atcggcttta gaaaactttg
     2461 tgatttttga ccgcgcttca aaattaaagg atttccctga catcaaaaac cgccctaccc
     2521 atttgctgtt caatatggca acaggtgctg gcaaaacgat gatgatggcg gcgttgattt
     2581 tgtattattt tgaaaaaggt tatcggcatt ttttgttttt cgtcaatcaa aataatatcg
     2641 tggataaaac ggaaaataat tttattgacc cggcgcacgc aaaattttta tttaccgaga
     2701 agattttgca aggtgatacg gtaattccta ttcgcaaagt ggaaatgttc agcccacatt
     2761 cagacggcat tgaaattaaa tttaccagca ttcaaaaact gtataacgat atccacaccg
     2821 agcgagaaaa ccaaaccaca ttggcagatt tgcacaaatt gaaccttgtg atgctgggcg
     2881 atgaagcaca ccatttaaac gcacaaacca aaggcaaaaa acaagacgag ttggatttgg
     2941 aagtggaatt aaaagccaat gcaggcaatg ccgaaattga acgcaaaggc tgggagcata
     3001 tggttttgga attgttgctc aataaaaatg gcaatcccgg ccaaaatgtg ctgttggaat
     3061 ttaccgccac gctgcctgaa aatgctgacg tgcaacaaaa atatgcggac aaaatcatca
     3121 caaaatttgg cttaaaagag tttttgcaaa aaggttatac caaagaaatc aatttggtat
     3181 ccagtacgct gaataagaaa gagcgggtgt tgcacgcttt attatttgct tggtatcgcc
     3241 atcaaatcgc gttgaaatac ggcattgcca atttcaagcc tgtgatgttg tttagaagta
     3301 agacgattga tgaatcaaaa gcggattatt cggcattttt aaattgggca gaaaatgtac
     3361 aagcggatga ttttgagttt ttagatacgt tttcaggcag cctgaataat agcgacaacg
     3421 ccaacgaaca aggcaaaacc cgaaccgaac aagccctaaa atttatgcag gaaaatggct
     3481 ttgagtttgc acatttggca aattgggtga aacaaaatta tcaaaaacat aatgtgatta
     3541 ttaccaactc cgaaaccaac aaaaacaaaa ctgaaaaaac cgacagcgaa accgaaaaat
     3601 tgctaaacaa tttggaagcg gctgataatc caatccgtgc catttttacg gtagatagat
     3661 taaccgaagg ttgggacgtt ctgaatttgt ttgatattgt gcgtttgtat gaagggcaaa
     3721 atggcggtgg ttcaaataaa aaatcaggca aaacggctgc cgctaccgta tcggaaaagc
     3781 agttgattgg tcgtggcgtg cgttattttc catttgcgtt tgaaggtaaa cagccgaata
     3841 aacgcaaatt tgacaacgac atgcaacacg aattgcgtat cttggaagaa ttgttttatt
     3901 acacgcacga tgagcaatcc cgctatattt cagaactgaa aaatgagttg cgaaaagacg
     3961 gttatttgcc tgaaaaagac gatgataagg tgttggcaac atttaaactc aaatctgaat
     4021 ttgccgataa taaggatttt agagagttgt taatttgggc gaataaaaaa atccccaacc
     4081 caaacgccaa aagcaataat gcagacagcc tgcaagccaa tccgcaaacg ctttcattcc
     4141 aagttcacgg taatcaactg ttgcaggaaa cgcaatttac agccgatgaa aatgatgaaa
     4201 cagcccgaca aatcggcaca caaaataatt ttactcaaac cataaaaatg agtgaaatgg
     4261 agcggcacat tttcaataaa gcattacata tcaaagggaa aaatagtcaa tctttattcc
     4321 atttcaaccg tttgcaaagc aagctcgaca ttcaaaatcg caatgaattg caaaataact
     4381 tgttgaaaga ctggcaaatt gaatttttag gattagggca agacaaacag gttcgcttgg
     4441 acgataaact tgcaggctgc ctgaaaatct tggaaatggt tgaaaaacat ctgaatgaaa
     4501 gtgatatgcc gtttatcggt acaaaagaat ttacacctaa aaaattatgg gaaattttcg
     4561 gcacgccaaa acaaaaatgg gtcaaaaaag atgatgtaaa aacgactatt gccacgcaaa
     4621 atgattggta tgtgatgaat aattttgccg gaacgggttt ggaagaagcg ttaattcaat
     4681 ttatttcagc gcgtttgggc gatttgaaat ctaaatatga cgttcattta atccgtaatg
     4741 aagaagtgtt taaattgaat aactttgccg atggtgaagg atttatgccg gactttattt
     4801 tattgctgaa agataaacaa aaatcttctt ccaatggcgt gaaggacttt ttgcattacc
     4861 aaattttcat tgagcccaaa ggtgagcatt tagtggaaac tgatggatgg aaagaagaat
     4921 ttttagaagc catcactgtg gaatatggga tggataagat cctgcaaaaa gatacaccgc
     4981 attatcgttt gatcggttta ccgtttttta ctgatcatca gaaaaatgga caatttacgg
     5041 agtcattccc tttaggtgcg gcatcgcttg aaaaaatgaa gtagtgcatt gcaggcaacc
     5101 ccgtttgaca aaacttcctt tacaaaaggg cgttttgtca gatatttaat caacacatta
     5161 ttaaaataca gccaaatttt aatgccgtcc gaaccctgtg ttcagacggc atcgtatttt
     5221 tcagtatcta aaccgtttcc ctgccccaat ctttgcctct caaaatcgaa gcatcgacat
     5281 cttgaatatc gcggtgtccc gtaaacgcca tagatatatc catttcttta tacaggattt
     5341 ccagcgcacg ggttacgcct tcttcaccat acgcacccaa accatacaga aacgcccgac
     5401 ctatcattgt acctttcgca cccaaagccc acgccttcaa aatatcctga ccgctgcgga
     5461 tgccgctgtc catccaaact tcgatgtcgc tgccgactgc actaacgatg tcgggcaagg
     5521 ctttgatggc ggacacggta tcgtcgagct ggcggccgcc gtggttggaa acgaccaatg
     5581 cgtccgcacc gcttttcgct gctttttccg catcttcagg ttccataatg cctttgataa
     5641 tcagcttgcc accccacaaa tctttaatgc gtgcaacgtc gtcccagctc aggcgcgggt
     5701 cgaattgttc ggaagtccat gaagacagcg aggacaaatc gccgacgttt ttggcgtgtc
     5761 caacgatatt gcggaacgtg cggcgttccg tgttcagcat tttcatacac cattcgggct
     5821 tggtcgccag attgattaaa ttggcgatgg tcggtttcgg cggcgcggac aggccgtttt
     5881 tgatgtcttt gtggcgttgc cccaaaacct gcaaatcagc ggtcaatacc aatgccgaac
     5941 atttggcatc cttcgcgcgc ttaatcaggt tttccataaa ctcgcggtcg cgcatcacat
     6001 aaagctgaaa ccaaaatggt gcggaagtgt tctcggcaac gtcttcaatc gagcagatag
     6061 acatcgtgga cagcgtaaac ggaatgccga acttctccgc cgcccgcgcc gccaaaattt
     6121 caccgtcggc gtgtgccata cccgtgaaac ccgtcggcgc aatcgccacc ggcattttca
     6181 catcctgccc gatcattttg gtctccaggc ttcggccttc catattgacc aatacctttt
     6241 gtcggaagcg gatgtctttg aaatccgaag tgttttcacg gtaggtagtt tctgtccacg
     6301 aacccgaatc gatgtaatcg taaaacatac gcggcatttt gcgcttggca acgcggcgca
     6361 agtcttcgat gcaggtcatt ttgctcaaat cacgtttcat ttgtcgcccc ctgaatacct
     6421 gaataacttt atatgaaata gataatgtat atcaatattg attataaggc aaatcatttc
     6481 aacatttact gcacccgccg ccgctcccac ttcaagcggc ataaggttta gaattcaaaa
     6541 ataatatatt aaaatcaaaa tattaaaaat caatcaatct gccgatttaa acagccaatc
     6601 gcgcaatccg cccccatact tgactgaaac actcagatat tggacaattc cgcccactat
     6661 aaaaaaagcc gacacgggca accaccacca tgagactgac caccaaaggg cgttttgccg
     6721 ttaccgctat gctggatttg gcgatgaacg cgcaaaccgg cgccgtcaaa ctcagtgcca
     6781 tcagcgaacg ccaaaacata tccctctcct atctcgagca attgttcggc aaactccgcc
     6841 gcgccggact tgttgaaagc ctgcgcgggc ccggcggcgg ctacatcctc gccgcaccgg
     6901 cagcacgcat caacatcgcc caaatcatcg ccgccgccga agaccggctg gacgcaaccc
     6961 aatgcggcag caaagccaac tgccaccacg gcgcgccctg cctgacgcac gacctttggg
     7021 agaatttaaa caaaaccatc aacgactacc tcggcagcgt taccctgcaa agcatcatcg
     7081 aacagaaaaa caacggcgac ggcagccgcg tcgtccaatt tacacacatc cattaaataa
     7141 cacccgaaaa agaaagagca aaccatgacc gtcaaaaccc ccgtttacct cgactacgcc
     7201 gccaccacca ccgttgacaa acgcgttgcc gaaaaaatga ttccctatct gaccgaaacc
     7261 ttcggcaacc ccgcctccaa cagccacgca ttcggctggg aagcagaaga ggccgtcgaa
     7321 aaagcccgcg ccgacatcgc tgccctgatt aatgccgacc ccaaagaaat cgtcttcacc
     7381 agcggcgcga ccgagtccga caacctcgcc atcaaaggcg cggcaaactt ctacaaaacc
     7441 aaaggcaaac acctcattac cgtcaaaacc gaacataaag ccgttttaga tacaatgcgc
     7501 gaactcgaac gccaaggttt tgaagtaacc tacctcggcg ttcaagaaaa cggtttgatt
     7561 gatttggaag aactcaaagc cgccatccgc gacgacacca tcctcgtttc cgtaatgtgg
     7621 gcaaacaacg aaatcggcgt ggtgcaagac attcccgcca tcggcgaaat ctgccgcgaa
     7681 cgcaaaatcg tcttccacgt cgatgccgca caagcctgcg gcaaagtgcc tgtcgatgtc
     7741 gaagccgcca aaatcgacct gctctcgatg tccgcgcaca aagtgtacgg ccccaaaggc
     7801 atcggcgcgc tgtacgtccg ccgcaaaccc cgcgtccgcc tcgaagccca aatgcacggc
     7861 ggcggccacg aacgcggctt ccgcagcggc acattgccga cccaccaaat cgtcggtatg
     7921 ggcgaagcct tccgcatcgc caaagaagaa ctcgaacaag atatggcgca ctaccgcaaa
     7981 ctgcgcgata ttttcctcaa aggtatcgaa ggcatcgaag aagtctatat caacggcgac
     8041 ctcgaacatc gcgtcccgaa caacctaaac gtcagcttca acttcgtcga aggcgaaagc
     8101 ctgattatgg cagtgaaaga actcgccgta tccagcggct ccgcctgcac ctccgcctcg
     8161 ctcgaaccca gctacgtcct gcgcgcattg ggcagaaatg acgaattggc gcactcgtcc
     8221 ctgcgcatca ccttcggtcg catgaccact gaagaagaag tgcaatttgc cgccgaactg
     8281 attaaatcca aaatcggcaa actgcgcgaa ctgtcgccgt tgtgggaaat gttcaaagac
     8341 gggattgatt tgaactcgat tgaatgggcg gcgcattaaa gcgtaccaac atgccatccg
     8401 aaccttcaga cggcattcca aaaacaaagc aatcaagaga aaaatatgaa cgaacaagat
     8461 ttagatttgg acaacctcga caacctgctt gacgatttcg acggcgttac cgtggaaggc
     8521 ggcgtcgatt cggaaaacga cgacggctgt gaaggcggag cgtgcaaaat ctaactcagg
     8581 ccgtctgaaa accggcaaac aaaccactta aatcatcaaa acattaagga aaccacatca
     8641 tggcatacag cgataaagta atcgaccact acgaaaatcc ccgcaacgtc ggtactttcg
     8701 acaaaaacga cgaatctgtc ggcaccggca tggtcggcgc accggcctgc ggcgacgtga
     8761 tgcgcctgca aatcaaagtg aacgatgaag gcatcatcga agatgcgaaa ttcaaaactt
     8821 acggctgcgg ttccgccatc gcttcgtcca gcctgattac cgagtgggtt aaaggcaaaa
     8881 gcctggatga cgcgctggca atcaaaaaca gcgaaatcgc cgaggagttg gaattgccgc
     8941 cggtaaaaat ccactgctcc atcttggctg aagatgcggt aaaagcggcc gttgccgact
     9001 accgcaaacg tcaggaaaac agataaagcc cttcagacgg catcgtcccg caatgccgtc
     9061 cgaaccaccc gccgcttcag gtccgtccgg ggcggtacaa caaggaagaa atatgattac
     9121 ccttaccgag aatgccgcaa aacacatcaa tgactatctc gccaaacgcg gcaaaggctt
     9181 gggcgtacgc ttgggtgtga aaaccagcgg ctgctcgggg atggcgtaca accttgaatt
     9241 tgtcgacgaa gccgatggcg acgacctgat tttcgaagga cacggcgcgc gcatttatat
     9301 cgatccgaaa agcctggttt atctggatgg cacgcaagtc gattacacca aagaaggttt
     9361 gcaggaaggt ttcaaatttg aaaaccccaa tgtcaaagac tcctgcggct gcggcgaaag
     9421 cttccatgtt taagacataa aaatggcggg accgtatcaa aaccgtcccg ccattttttc
     9481 gcttcccgcc tgttgtagct gcctttgcct tttcttttcc gttccacctt gtgtcggaac
     9541 aaatcagatt tcactaaggc ttttaaagca ttgtcgcgta ttttgccttt attgtgctgc
     9601 actttgccgc ccatattcag tcctttcgtt taagaagcgg cagattataa ggcaaaaaca
     9661 gttttctgcc aaaatcttac atttatcatc ctactatgtc ccaatatttc accctcttcc
     9721 ggattgaacc cgctttcgat atcgacaccg aaaacttgga acaaacctac cgcgccttgg
     9781 ccgcccgttt ccatcccgat aaattcgctt cagcttccgc ctttgagcaa aagcaggcag
     9841 tgatgatgtc ttccaccatc aacgatgcct accgcacctt gaaaaacccc atcgaccgcg
     9901 ccgcctacct gctgaaaaca tcgggcatcg atgccgacgc gccggagcat accgctttcg
     9961 cccccgaatt ccttatgcag caaatggaat ggcgcgaaac gctgatggag gcacgggcag
    10021 gcaacgacct tgaatccttg aaaaatctcg acaacgaaat ccgcgacgaa caagaaaaac
    10081 tgttctgcgg tctgaaacag tcgtttgccc gacaagatta cgacacagcc gcacaacaag
    10141 tccgccaagg caggtttctc gacaaactcc gcaacgaaat ttcctcggca ttataatccg
    10201 caccgtgttt cagacggcgt aaccgccgca ccgttccgcg tcaaaatatg ctaaaataag
    10261 caacaatttt ttgccatacg aaacattgaa accatgaccg acgcaaccat ccgccacgac
    10321 cacaaattcg ccctcgaaac cctgccggta agccttgaag acgaaatgcg caaaagctat
    10381 ctcgactacg ccatgagcgt cattgtcggg cgcgcgctgc cggacgttcg cgacggtctc
    10441 aagccggtac accgccgcgt actgtacgcg atgcacgagc tgaaaaacaa ctggaatgcc
    10501 gcctacaaaa aatcggcgcg cattgtcggc gacgtcatcg gtaaatacca cccccacggc
    10561 gataccgccg tatacgacac catcgtccgt atggcgcaaa atttcgctat gcgttatgtg
    10621 ctgatagacg gacagggcaa cttcggatcg gtggacgggc ttgccgccgc agccatgcgc
    10681 tacaccgaaa tccgcatggc gaaaatttcc cacgaaatgc tggcagacat tgaggaagaa
    10741 accgtcaatt tcggcccgaa ctacgacggt agcgaacacg agccgcttgt actgccgacc
    10801 cgtttcccca cactgctcgt caacggctcg tccggcatcg ccgtcggcat ggcgaccaat
    10861 atcccgccgc acaacctttc tgataccgtc aatgcctgcc tgcgcctgct cgatgcaccc
    10921 gacaccgaaa tcgacgaact gatcgacatt atccaagccc ccgacttccc gaccggggca
    10981 accatctacg gcttgagcgg cgtgcgcgaa ggctataaaa caggccgcgg ccgcgtcgtt
    11041 atgcgcggta agacccatat cgaacccata ggcagaaacg gcgaacgcga agccatcgtt
    11101 atcgacgaaa tcccctatca ggtcaacaaa gccaagctgg tcgagaaaat cggcgatttg
    11161 gttcgagaaa aaacactgga aggcatttcc gagctccgcg acgaatccga caaatccggt
    11221 atgcgcgtcg ttatcgagct gaaacgcaac gaaaatgccg aagtcgtctt aaaccaactc
    11281 tacaaactga ctccgctgca agacagtttc ggcatcaata tggtggtttt ggtcgacgga
    11341 caaccgcgcc tgttgaacct gaaacagatt ctctccgaat tcctgcgcca ccgccgcgaa
    11401 gtcgttaccc gacgtacgct tttccggctg aagaaggcac gccatgaagg gcatatcgcc
    11461 gaaggcaaag cggtcgcact gtccaatatc gatgaaatca tcaagctcat caaagaatcg
    11521 cccaacgcag ccgaggccaa agacaaactg cttgcgcgtc cttggcgcag cagcctcgtt
    11581 gaagaaatgc tgacgcgttc cggtctggat ttggaaatga tgcgtccgga aggattggct
    11641 gcaaacatcg gcttgaaaga gcaaggttat tacctgagcg agattcaggc agatgctatt
    11701 ttacgcatga gcctgcgaaa cctgaccggc ctcgatcaag aagaaattgt cgaaagctac
    11761 aaaaacctga tgggtaaaat catcgacttt gtggatatcc tctccaaacc cgaacgcatt
    11821 acccaaatca tccgcgacga actggaagaa atcaaaacca actatggcga cgaacgccgc
    11881 agcgaaatca acccgttcgg tggcgacatt gccgatgaag acctgattcc gcaacgcgaa
    11941 atggtcgtta ccctgacaca tggcggctat atcaaaaccc agccgaccac cgactatcag
    12001 gcgcagcgtc gcggcgggcg cggcaaacag gcggctgcca ccaaagacga agactttatc
    12061 gaaaccctgt ttgttgccaa cacgcatgat tatttgatgt gctttaccaa tttgggcaag
    12121 tgtcattgga ttaaggttta caaactgccc gaaggcggac gcaacagccg cggccgtccg
    12181 attaacaacg tcatccagtt ggaagaaggc gaaaaagtca gcgcgattct ggcagtacgc
    12241 gagttccccg aagaccaata cgtcttcttc gccaccgcgc agggaatggt gaaaaaagtc
    12301 caactttccg cctttaaaaa cgtccgcgcc caaggcatta aagccatcgc gctcaaagaa
    12361 ggcgactacc tcgtcggcgc tgcgcaaaca ggcggtgcgg acgacatcat gctgttctcc
    12421 aacttaggta aagccatccg cttcaacgaa tactgggaaa aatccggcaa cgacgaagcg
    12481 gaagatgccg acatcgaaac cgaaatttca gacggcatcg aagatgaaac cgccgacagc
    12541 gaaaacgcac tgccgagcgg caaacacggt gttcgcccgt ccggtcgcgg cagcggcggt
    12601 ttgcgcggta tgcgcctgcc tgccgacggc aaaatcgtca gcctgattac cttcgcccct
    12661 gaaaccgaag aaagcggttt gcaagtttta accgccaccg ccaacggata cggaaaacgc
    12721 accccgattg ccgattacag ccgcaaaaac aaaggcgggc aaggcaatat tgccattaac
    12781 accggcgagc gaaacggcga tttggtcgcc gcaaccttgg tcggcgaaac cgacgatttg
    12841 atgctgatta ccagcggcgg cgtacttatc cgcaccaaag tcgaacaaat ccgcgaaacc
    12901 ggccgcgccg cagcaggcgt gaaactgatt aacttggacg aaggcgaaac cttggtatcg
    12961 ctggaacgtg ttgccgaaga cgaatccgaa ctctccgacg cttctgtaat ttccaatgta
    13021 accgaaccgg aagtcgagaa ctgaaaatca tctcccgatg ccgtctgaag attcagacgg
    13081 catttatttt atccctcatc cgtcatccag cttctcacaa tatagcggat tatagtcaat
    13141 taaaaacaag gggctgtcct agataactag ggaaattcaa attaagttag agttgcccct
    13201 atgagaaaaa gtcgtctaag ccggtataaa caaaataaac tcattgaact
//
LOCUS       DAWKOD010000046        12870 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000046 DAWKOD010000000
VERSION     DAWKOD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.046"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..361)
                     /locus_tag="WJK73_000963"
     CDS             complement(53..361)
                     /locus_tag="WJK73_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557461.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WJK73_000964"
     CDS             complement(345..647)
                     /locus_tag="WJK73_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ7557462.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WJK73_000965"
     CDS             complement(721..3084)
                     /locus_tag="WJK73_000965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ7557463.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVNGLHILPEDAPVGINIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRSRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGYPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGM
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WJK73_000966"
     CDS             complement(3120..3638)
                     /locus_tag="WJK73_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557464.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WJK73_000967"
     CDS             complement(3645..4709)
                     /locus_tag="WJK73_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7557465.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJK73_000968"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJK73_000968"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7557466.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WJK73_000969"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WJK73_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ7557467.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WJK73_000970"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WJK73_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ7557468.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WJK73_000971"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WJK73_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ7557469.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WJK73_000972"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WJK73_000972"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ7557470.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9445..9521)
                     /locus_tag="WJK73_000973"
     tRNA            complement(9445..9521)
                     /locus_tag="WJK73_000973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9485..9487),aa:Val,seq:gac)
     gene            9700..10815
                     /gene="tgt"
                     /locus_tag="WJK73_000974"
     CDS             9700..10815
                     /gene="tgt"
                     /locus_tag="WJK73_000974"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ7557471.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            10825..11907
                     /locus_tag="WJK73_000975"
     CDS             10825..11907
                     /locus_tag="WJK73_000975"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ7557472.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12098..12499
                     /locus_tag="WJK73_000976"
     CDS             12098..12499
                     /locus_tag="WJK73_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7557473.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12562..12852)
                     /locus_tag="WJK73_000977"
     CDS             complement(12562..12852)
                     /locus_tag="WJK73_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557474.1"
                     /translation="MSKYFTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLKLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3294 a         3398 c         2832 g         3346 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacagctctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgactccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgta atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccatgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggcg ggatatccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccacgcgccg ccatttcgtt gtaaacggca aaacgcgagc gacgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcagaccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acgcgccttt
     2161 ttcgtctgcc acgaccagcg tattttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 gaaaacgtgc atcggctggc cgatttccag cattacataa ttgccgatgt ccaccagcac
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cccttaacac tcaagcaatc
     2581 agcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcacg
     2641 gatattgata ccgacaggcg cgtcttcagg caaaatgtgc aggccgttca caccatcatc
     2701 aggcaaacct agttcattgg tcgaacacag cataccgttt gatggcacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacgatctga accaactctc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaacaacc acgcccgcaa aggcaggtgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggcggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatca attaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gccaaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagaca cattgtgaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctacttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cgggatcgat attcacattt ctcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaata tcgagggctt cggcggcaag tcgggcttgc agcttgactt
     5641 cgttgagggc ttcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggtttta ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatataccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttca atagaggcgc gtttcagacc gttcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtca cggtacgcgt attgaccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgagcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcggataa ggcattaggg ggttttaaaa
     6841 catcagtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct tttacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttaatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacga caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttctcct tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataattacc aattgaaccg gagccaacca taacgggaat
     7801 gagcctgcat ggttctcaat cagaatgccg ataaaccgct ccaaagaacc taaaatggcg
     7861 cgatgcaaca taacaggacg cgcacggtcg ttgttttcag ttacatattc ggcattcaaa
     7921 cgctccggca atacgaaatc cagttgtaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctttgacat gatattcgat tttaggcccg taaaacgcac cctcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttt ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgagcctc gctgacgatt
     8221 tgatcttcgg tacagaaaat atgcgcatca tcctgcacaa aaccgcgaac ccgcatcaga
     8281 ccgtgcagcg caccgctcgg ctcattgcgg tggcaagaac cgaattccgc caaacgcatc
     8341 ggcaaatctc gatacgaacg caaaccgttg ttaaaaattt gaacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt gtctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca tgatttgagg cgttttgacc
     8521 tctttataac cggcggcatt cagctctttc cgcatatgct gctcaatcac ttgccacaaa
     8581 gcccagcctt tgggatgcca aaacaccata cccggtgctt cgtcttgcaa gtggaacaaa
     8641 tccagttgtt tgccaagctt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt agatgcgttg cagcatttca
     8761 ttgttgctgt cgccgcgcca gtatgcgccc gccaacttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cgggccgcgg cacatatcga catattcctg gtgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgcgcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga atcttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccttgc ctgccaccgc cgccttcgcc
     9301 aaaccggcac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatattcaac atcaaacgct ccgaaagatt aaaaaataac
     9421 ggcataaatg ccgttatggg gatttggtag gcacgattgg attcgaacca acgaccccca
     9481 ccatgtcaag gtggtgctct aaccaactga gctacgtgcc ttcaaagaat tttcgcattt
     9541 tatcgagtcg ttttaatttt tgcaagaggt atatgcattt ctttatgcaa cattgtggca
     9601 agatttgggg atgtcgtctg aaaaaccgtg ggaattcatt tataatgccg tcttttaaaa
     9661 catatttcga cccctgtcgc ccaactgaca ttcaatctta tgcttaaatt tactttacac
     9721 aaaaaagacg gatacgcccg acgcggcacg ctggagttga accacggcaa aattgaaacg
     9781 ccggtattta tgcctgtcgg tacttacggt tcggttaagg cgatgaaccc gcaaaacctg
     9841 cacgacatca aggcgcaaat tattttgggc aacacttatc atttgtggct gcgtccgggc
     9901 ttggaggtcg tcgaacagtt tggagggctt cacggcttta tcggctggga caaaccgatt
     9961 ttgaccgact cgggcggttt tcaggttttt tctttgtcgg atatgcgcaa gctgacggaa
    10021 gaaggctgta cgttcaaaag cccgatcaac ggcgacaagc tgtttttatc gcctgaaatt
    10081 tccatgaaaa tccaaacggt tttgaactcg gacatcgcga tgcagctgga cgaatgcacg
    10141 ccgggcgaag caacgcgcga acaggcgcga aaatcgctgc aaatgagcct gcgctgggcg
    10201 gaacggagca aaaaagcctt tgaagatttg aaaaacccga acgcactgtt cggcatcgtg
    10261 caaggcgcga tgtatgaaga tttgcgcgaa gagtcgctga aaggcttgga agagctcgat
    10321 ttccccggct tggccgtcgg cggcttgtcc gttggcgaac ctaagcccga aatgtaccgc
    10381 atgttgcgcg ccgtcggccc tatcctgccg gaacacaaac ctcattattt aatgggcgtc
    10441 ggtacgcctg aagacttagt gtacggcgtg gcacacggcg tggatatgtt cgactgcgtc
    10501 atgcctactc gcaatgcgcg taacggctgg ctgtttaccc gttttggcga tttgaaaatc
    10561 aaaaacgcca aacacaagct cgacaagcgt ccgatagacg aaagctgcac ctgctacgcc
    10621 tgccaaaact tcagccgcgc ctacctgcac catctgcacc gcgccggcga aatcttgggc
    10681 gcacagttga acaccatcca caatctgcat ttctaccaag tcattatggc ggaaatgcgc
    10741 gaagccgtcg aacaaggcaa atttgccgac tggcaagcac aattccacga aaaccgcgca
    10801 cgcggcacag actgactccc tgcaatgccg tctgaagccg cttcagacgg cattttgccg
    10861 cgttacccta cccgcactat ttttcagaaa ccgatcatgc tcccattcct tccagaaccc
    10921 tccctttcct atacgcaaca aaaccgtacc gccgttttgc tgttgaacct cggcacgccc
    10981 gacgcgccga ccgcgcaggc ggtacgacct tatctgaagt cctttctgac cgaccggcgc
    11041 gtcgtcgaac tgcccaaatg gctgtggtat cccattctgc atggtctggt actgacgctc
    11101 cgccccaaaa aaagcgcgca cgcctatgaa aaaatctggt ttaaggaagg ttcgccctta
    11161 gaagtttaca cagcacgcca agctgccgcg cttgccaaac gtatgcccga cctcatcgtc
    11221 cgccacgcca tgacctacgg caatccttcg gtggcagacg tattgtcgga actgaaggcg
    11281 caaggcgcgg gcagactgct ggtcatcccg atgtacccgc aatatgccgc ttccagcagc
    11341 ggagcggcag tagataaggt gtgcgaacaa ttactgctgc aacggaacca aatgagcgtc
    11401 cgcaccgttt cgcgctttta tgacgatacc ggctacattg atgcgatgaa aaaccacatc
    11461 ctccgatatt gggcggaaca cggacgtggg aaaaaactga tgttgagttt tcacggcgta
    11521 ccgcagaagc accacgacct cggcgacccc tatcccgacg agtgccgcca caccgccaaa
    11581 ctgcttgccg aagcactgga actgaccgaa gaccaatatg tcgtatcgtt ccaaagccaa
    11641 ttcggcaggg caaaatgggt tacaccgagc acgcaggatt tgttcggcaa actgcccaaa
    11701 caaggcgtaa ccgaactgga cgtattctgc ccgggctttt tggcagactg tttggaaacc
    11761 atggaagaaa tcgccctgat ggggcgtgaa cagttttacg aagcaggcgg caaaagctac
    11821 cgctacatcc cctgcctcaa cgacaacccc gactggatag acgcactcgt cgcacttgcc
    11881 gaagaaaacc ttggcggctg gcgttgaaac tgtttgaccc atgtctaaaa tgccgtctga
    11941 acgccgtgtt cagacggcat tttcccgcta ctttagcgta tgatggcatc tatgctggac
    12001 agcacatcaa gtgcaacctt accaccgatt cggcaaaaaa acctttcacc cgctacgcaa
    12061 aatcaacaac aaggtttcaa acattaagga aacaccaatg aacacaaccc gactgccgac
    12121 cgccctcgtc ttgggctgcc tctgcgccgc cgcttctgcc gccgacaaca gcatcatgac
    12181 aaaaggacaa aaagtgtacg aatccaactg cgtcgcctgc cacggcaaaa agggcgaagg
    12241 tcgcggaacc atgtttccgc cgctctaccg ctccgacttc atcatgaaaa aaccgcaggt
    12301 gctgctgcac agcatggtca aaggcatcaa cggtacaatc aaagtcaacg gcaaaaccta
    12361 caacggattc atgcccgcaa ccgccatcag cgatgcggac attgccgccg tcgccactta
    12421 tatcatgaac gcctttgaca acggcggcgg aagcgttacc gaaaaagacg taaaacaggc
    12481 aaaaggcaaa aaaaactaaa cagacaaaat gccgtctgaa accggcaatc cggcttcaga
    12541 cggcattcaa atcaaacctt tctaacgcgg tactttcagc cccatagacc cgatattgcc
    12601 gtccccgtca acctccttcg cggtcagcat aaagccgccg atgtcaatac gaccgcactc
    12661 gaccggctcc tgactgtcgg aacgcttatc gaaaagctcg gcaaggctca aaccctcttc
    12721 gcccgcttcc agcttcaaac cgtaagcaag tgccaaatcg tccgaacgtg ctgccggcga
    12781 aacgacaaac tcgccgaaga aatcaaaatg ctcacggaca ccgatatccg tctcggtaaa
    12841 gtatttcgac attttatagt gaactaaatt
//
LOCUS       DAWKOD010000047        12433 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000047 DAWKOD010000000
VERSION     DAWKOD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.047"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..809)
                     /locus_tag="WJK73_000978"
     CDS             complement(42..809)
                     /locus_tag="WJK73_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7557475.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJK73_000979"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJK73_000979"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ7557476.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQVNPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGKPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEVFCADYLYARLKTQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..2441)
                     /locus_tag="WJK73_000980"
     CDS             complement(1776..2441)
                     /locus_tag="WJK73_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7557477.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2619..4595
                     /locus_tag="WJK73_000981"
     CDS             2619..4595
                     /locus_tag="WJK73_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7557478.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4663..5079
                     /locus_tag="WJK73_000982"
                     /pseudo
     CDS             4663..5079
                     /locus_tag="WJK73_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
     gene            5079..5225
                     /locus_tag="WJK73_000983"
                     /pseudo
     CDS             5079..5225
                     /locus_tag="WJK73_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            5201..6409
                     /locus_tag="WJK73_000984"
     CDS             5201..6409
                     /locus_tag="WJK73_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7557479.1"
                     /translation="MLVIGIPMILVENVIGRRKGVNALDAFGGSMNGKPVAKIWKLVG
                     WAGLLGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWE
                     IAFYTFLFVVVNQWILVKGVIGGIEKAAKYLMPLLFLFLIAMVVRNVTLPGAMEGITF
                     YLKPDFSKITTELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTI
                     IAVLAGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALT
                     TSLTIYEVLITTIQEKTKIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSIFGKNIF
                     DAFDYISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLV
                     ILLIFVSNLF"
     gene            6653..7309
                     /locus_tag="WJK73_000985"
     CDS             6653..7309
                     /locus_tag="WJK73_000985"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ7557480.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            7465..9150
                     /gene="rpsA"
                     /locus_tag="WJK73_000986"
     CDS             7465..9150
                     /gene="rpsA"
                     /locus_tag="WJK73_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ7557481.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9161..9475
                     /locus_tag="WJK73_000987"
     CDS             9161..9475
                     /locus_tag="WJK73_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ7557482.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(9826..10233)
                     /locus_tag="WJK73_000988"
     CDS             complement(9826..10233)
                     /locus_tag="WJK73_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ7557483.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            10381..11490
                     /locus_tag="WJK73_000989"
     CDS             10381..11490
                     /locus_tag="WJK73_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ7557484.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGVGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            11499..12326
                     /gene="fghA"
                     /locus_tag="WJK73_000990"
     CDS             11499..12326
                     /gene="fghA"
                     /locus_tag="WJK73_000990"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ7557485.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         3258 a         3440 c         2903 g         2832 t
ORIGIN      
        1 ccacaaaagc gaatcatact gccacattcc gacaaccggt ttcagacagc gatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcatccgc cacgcaaacg aaatcgactc ttatcgtcaa ttttttcata
      181 gacacgatgc gccacaggca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatcg
      301 atggcggatt ggaacagcgc ggctttgaac ggcaaaagcc ccaatccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      601 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaaacc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg ggcaaccgcc gtcaataccg
      721 cgcaggtttt tcccggtttt gaacagccag accgccaaac ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg agcaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tcagtcctat
      901 ttctgacagt tcgggcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcact gggggcacgg ctgattgtgc cgcccgtaca ctgtatattc ctgttggaag
     1021 tagccgcttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgctgca acaccgcttt gacggtttca accaaaagcg cacactcttt ctttttcagg
     1141 cggttggcag gacggtgggg cgaaatgccc gccctaaaca ggctctcgtt ggcataaatg
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcca gcttgactgc ccgtttctgg
     1261 gttttcagcc ttgcatacag ataatccgca caaaatactt ccgacagagg ttcaggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg gcttgccgat acgtccgtcc gacggcgtaa aaatccgcaa actgcccgac
     1501 atccccaagt gaatcagcaa aatccccgtt tgaaaacgga caatcagata tttcgcccgc
     1561 ctgccgcaag acaaaacctg cctgccggac aaaatctccc ccaaatcggg attgacctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg tttttccttc aatatgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattcag gcataacccc tcccgacatt
     1741 ttttctgaca gatgccgtct gaaagacggc cgtccctaat ccgcaaccct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccacgcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccaa agcgtacact gcggcacatt gcggccacca acggtcgccc
     1921 gccttttcca taaaccgcca aaagcgtatt tgcccgatcg acgaaacggg cggcagatac
     1981 accataaatt tcccaaattc aatatcgaaa ccgacatccg caagctgcct tttcaactcg
     2041 ggcagcggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg aatacggatt gaaacccgtc agcatcaagc gtccgcacgg tttcaatatc
     2161 cggtgcgcct cggacaggat ttgcgaaggg ataccgcctt caagcgtatg cggaaaaagc
     2221 agcatatccg cagaaacatc cgccaaagcc atattctccg ccgacatcga catatctcgc
     2281 ggcacacaga caacatcttc agacaggctc agccacctgc cgcccacctg aaccgcacac
     2341 attcccgaaa aacggtatga atccagatac cgcccgaaga aatcctgttc caattttgca
     2401 acataccgcc ccatcgccgt atcttcaaac catgcatcca tattgcgtcc gtttcaaaca
     2461 gattgcctgc cgatattcta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc
     2521 atttccgaat ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca
     2581 tcggacgata aaatatgttt attccgccga tataaatcat gtccaaactc aaaaccatcg
     2641 ccctgaccgc gtcaggtctg tccgtttgtc cgggtttcct atacgcccaa aacacctcat
     2701 cacaccaaat cggtttggcg attatgcgct taaactcttc aatactcgac ctgcccccga
     2761 caaaacaata tttccaatcc ggcagcctgt ggagcgagct gcgccaaggc ttccggatgg
     2821 gcgaagtcaa tcccgaactg gtacgccgcc acgaaagcaa attcatcgca agccacagct
     2881 atttcaacag ggtcatcaac cggagtagac cctatatgta ccatatcgcc aacgaagtca
     2941 aaaaacgcaa tatgcccgcc gaagccgccc tgcttccctt catcgaaagc gcgttcgtca
     3001 ccaaagccaa atcacacgtc ggcgcatcgg gcctgtggca gttcatgccc gctaccggca
     3061 ggcattacgg cctggaaaaa acaccggttt acgacggcag gcacgacgtt tacgccgcca
     3121 ccgatgccgc actcaactat ctgcaatacc tctatggact gttcggcgac tggccgcttg
     3181 cctttgccgc ctacaactgg ggtgaaggca acgtcggacg cgccatcaac cgcgcccgcg
     3241 cccaagggct cgaaccgacc tacgaaaacc tgcgtatgcc caacgaaacg cgcaactatg
     3301 tccccaagct gctcgccgtg cgcaacatta ttgccactcc ccaatctttc ggcatgaata
     3361 tcagcgacat agacaacaaa ccctattttc aggcagtcga accggatcgt ccgctcgaca
     3421 acgaagccat cgcccggctt gccggcatca cgcaaagcga gctgctcgcc ctaaaccccg
     3481 cattcaacgt ccccgcgttt atccccaaaa gcaaacgcaa actgctgctt cctgtcgcgt
     3541 ccgtacaaac cttccaaagc aactacctca acgccgcacc cgacagcctg ttttcatggg
     3601 aagtctatac gcctgccgcc aaaaccagcc tgtccgacat ctcgacggca accggcatga
     3661 gcattgccga catcaaacgc ctcaacaacc tgaacggcaa ccttgtcaac gcaggacgca
     3721 gcatccttgt cgccaagaac ggcaaaaccc ttcagacggc atcggaatcc gtcgtttcca
     3781 tcgacatcga caatacaccc gacacctacc gttccaatat gccggcaggc acggtgaacg
     3841 tcggcattgc ccgaatccga cccgccgccg cacagacagc ggacattacc gtcgcacctt
     3901 tgccgcagaa aaccgtccgt acggaacccg atccccttgt ccgtattgcc gaacctgccc
     3961 ttgcgacagc cgcagcgcaa cctcaaaccg aaaaacagac cgccatgccg tctgaaaccc
     4021 aaaccgcaac actcgcgcag atcgtccccc aaaacgacat gcaggcggca gacgaactca
     4081 tgcagcttgt tgcccgaaac aacctgcgcc gacaggctga agaaaccatc tctgccgtca
     4141 tcggcacgcc tgacacagtt gccgaacaca acatttcctc atctccgcaa cataccgttg
     4201 ccgccgacgg caaacgccgg gtacgtttgg aaacgcgcgt agccaaagct gccgacggcg
     4261 aagccgaaat ctccccgctc catgccagca tccaccgcgt tgtagaaggc gacaccctgt
     4321 tcaacattgc caaacgctac aacgtcagtg tagccgacct gattgtcgcc aacaacatca
     4381 aaggcaacac catccaaaaa ggacaggtac tccgcctggc gcaggcagcc cctgcccaaa
     4441 cccgtattga aaaagtatcc tacaccgcgc gcaaaggcga caccttcaaa agtatcgccg
     4501 cgcgcttcaa tatccatatc gacgacatcc gccggctcaa tcccaacctg aacaccatca
     4561 atccgggaca gagggtcaaa ctgattggaa gctgattcgg atacggcaca tgacaggact
     4621 ttctcagtcc tgtctttttc atcccacatt tcccatacca tcatgaaact tatcaaatac
     4681 ctgcaatatc aaggcatagg aagccgcaag cagtgccaat ggctgattgc cggcggttat
     4741 gttttcatca acggaacctg catggacgac accgatgcag acatcgattc ctcatccgtc
     4801 gaaacgttgg atattgacgg ggaagcagta accgtcgttc ccgaacccta tttctacatc
     4861 atgctcaaca agcctgaaga ttacgaaact tcgcacaaac ccaagcacta ccgcagcgta
     4921 ttcagcctgt tccccgacaa tatgcggaac atcgatatgc aggcggtcgg caggctggat
     4981 gcggatacga ccggcgtatt gctgattacc aacgacggca aactgaacca caacctgact
     5041 tcgccgagca gaaaaattcc caagctatac gaagtaacca ccatcaagcc tttatcctga
     5101 ccattgccgg cgcgactgtc ggtttcggcg cgacgtggcg tttcccgtat ttggtcggtg
     5161 aaaacggtgg tgcgcgtatg tgtttttatt ctgtatcgcg atgctggtta tcggcatccc
     5221 gatgattttg gtggaaaatg tcatcggacg gcgcaaaggc gtgaacgcgc tggatgcgtt
     5281 cggcggttcg atgaacggca aacccgtcgc taaaatttgg aagctggtcg gctgggcagg
     5341 actgctcggc gcgttcggca ttatggctta ttacatggta ctcggcggtt gggtcatcag
     5401 ctatatcgtt aatattattg gcggaaatct gaatatttcc agccccgtcg acggtgtggt
     5461 tacaaaaggc ttttttaccg aacacattga aaacagccct tgggaaattg cgttctacac
     5521 tttcttgttt gtcgttgtga accaatggat tttagtcaaa ggcgttatcg gcggtattga
     5581 aaaagcggca aaatacctga tgccgctgct gtttttgttc ctgattgcca tggtcgtacg
     5641 caacgttacc ctgcctggcg caatggaagg gattactttc tatctgaagc ctgatttcag
     5701 caagattacc accgaactgt tcgtcttcgt tttgggacaa gtatttttcg ccttgagctt
     5761 gggtttcggc gtgatgatta ccttatccag ctatctggat aaaaacgaaa atctggttca
     5821 gacggcagtt atcacggcaa ttaccaatac catcatcgcc gtacttgcgg gctttatgat
     5881 tttcccgtcg ctcttcagct tcggcgtcgc gcccaattcc ggcccgactt tggtgttcca
     5941 aagtttaccg attgtgttct cacatatgtg ggcaggatct gtgttcgccg tcattttctt
     6001 ctctctactc ctgattgccg cgttgacaac ttcgctgacg atttatgaag tgttgattac
     6061 gaccattcag gaaaaaacca aaatccgccg caccgccgcg attacgattg tattggctgc
     6121 catcttcatc ttcggcaaca tcccgtccat tctgagctat ggtccgtgga aagatgtttc
     6181 catatttggc aaaaatattt ttgacgcttt tgactacatc agcggcaaca tcttgtttat
     6241 gctgaccgcg ctcggctccg cgctgtttgt cggttttgtg atgaaggacg aagcgaagga
     6301 agaattgctt tacaaaggca accatacgac ggtcaatatt tggtttgctt atgtgaaata
     6361 tcttgtgccg ttggtgattt tgctgatttt tgtcagcaac ctgttctaat ctgcagcaat
     6421 cgatgccgtc tgaaggccat acctctttca gacggcattg ccttgatgcc cgccacaata
     6481 cccgcaacgc cggaatccga accggcttgc cggcttctgt cggatgtttt cgggcaggcg
     6541 gcgttccgtc tgcttagaca atcgtccttt aaaacaggta gaatccgccc caacgggaaa
     6601 cacatccttc agacggcaaa acccataccc caaaccatca ggaatccccc ttatgaacag
     6661 acaaaaagtc atcgccatcg acggcccggg cgcatcgggc aaaggcacgg ttgccgcccg
     6721 cgttgccgcc gcattgggat acgattatct cgataccggc gcactctacc gcctgaccgc
     6781 cctatatgca caaaaacaag gcgtgggatg gcacgatgaa gaaaacgttt ccgaactggc
     6841 aaaaaaactg cccgccgtat tttcaggcag ccgcatcctg ctcggcggcg aagacgtttc
     6901 agacggcatc cggacagaag ccatcggcat gggcgcatcc gcagtcgcac agttgcctaa
     6961 agtccgcgcc gccctgctgc aacgccaacg cgattttctg accgaaaaag gactggttgc
     7021 cgacggacgg gacaccggat cggtcgtctt cccccaagcc gaacttaaaa tcttcctgac
     7081 ggcagaatcc aaaatccgtg ccgaacgccg cgccaaacaa atcggcatcc cctgcgaagg
     7141 tttggcattc gagcgcatcc tgtccgacat cgaagccaga gacgaggcag accgaaaccg
     7201 caaagttgcc cccctgaaac aacagcccga tgccctgctt ttggacacaa gccgcctgac
     7261 tatagaagaa actgtaaaaa aagtgcttga ttggtatcgt gaagtttaaa ttttcaggta
     7321 taatcgcaca aattacgttt cagacggcat aaaaatcccc catatgccgt ctgaaacctt
     7381 atgtacccgt ctgccctgcc aaggacggca gatatccacc aacccacccc gcaccccttg
     7441 gcggtgtacc gaaaagagtt atatatgtct atggaaaatt ttgctcagct gttggaagaa
     7501 agctttaccc tgcaagaaat gaacccgggt gaggtgatta ccgctgaagt agtggcaatc
     7561 gaccaaaact tcgttaccgt aaacgcaggt ctgaaatcag aatccctgat tgatgtagct
     7621 gaattcaaaa acgctcaagg cgaaattgaa gttaaagtcg gcgacttcgt taccgttacc
     7681 atcgaatccg tcgaaaacgg cttcggcgaa accaaactgt cccgcgaaaa agccaaacgc
     7741 gctgccgatt ggattgccct ggaagaagcc atggaaaacg gcaacatcct gtccggcatc
     7801 atcaacggca aagtcaaagg cggcctgacc gttatgatta gcagcatccg cgcattcctg
     7861 ccgggttctt tggtcgacgt acgtcctgta aaagacactt ctcacttcga aggtaaagaa
     7921 attgaattca aagtgatcaa actggacaaa aaacgcaaca acgttgttgt gtcccgccgc
     7981 gccgttctgg aagctacctt gggtgaagaa cgcaaagccc tgctggaaaa cctgcaagaa
     8041 ggctccgtca tcaaaggcat cgttaaaaac attaccgatt acggtgcatt cgttgacttg
     8101 ggcggcatcg atggtctgct gcacatcacc gacttggcat ggcgccgtgt gaaacacccg
     8161 agcgaagttc tggaagtcgg tcaagaagta gaagctaaag tcctgaaatt cgaccaagac
     8221 aaacaacgcg tttctctggg tatgaaacaa ctgggcgaag atccttggag cggcctgacc
     8281 cgtcgttacc cgcaaggtac ccgcctgttc ggtaaagtat ccaacctgac cgactacggt
     8341 gcgttcgttg aaatcgaaca aggcatcgaa ggcttggtgc acgtttccga aatggattgg
     8401 accaacaaaa atgtacaccc gagcaaagtt gtacaattgg gcgacgaagt cgaagtaatg
     8461 atcctcgaaa tcgatgaaag ccgccggcgt atctctttgg gtatgaaaca atgccaagcc
     8521 aatccttggg aagaatttgc cgccaaccac aacaaaggcg acaaaatctc cggcgcggtt
     8581 aaatccatta ccgatttcgg cgtattcgtc ggtctgcctg gcggcatcga cggtttggtt
     8641 cacctgtccg acctgtcctg gaccgaatcc ggcgaagaag ccgtacgcaa atacaaaaaa
     8701 ggcgaagaag tcgaagccgt cgtattggca atcgacgtgg aaaaagaacg catctccttg
     8761 ggtatcaaac aactggaagg cgatccgttc ggcaacttca tcagcgtgaa cgacaaaggt
     8821 tctttggtta aaggttccgt gaaatctgtt gacgccaaag gtgctgttat cgccctgtct
     8881 gacgaagtag aaggctacct gcctgcttcc gaatttgcag ccgaccgcgt tgaagatttg
     8941 accaccaaac tgaaagaagg cgacgaagtt gaagccgtca tcgttaccgt tgaccgcaaa
     9001 aaccgcagca tcaaactttc cgttaaagcc aaagatgcca aagaaagccg cgaagcactg
     9061 aactccgtca atgccgccgc caatgcgaat gccggcacca ccagcttggg cgacctgctg
     9121 aaagccaaac tctccggcga acaagaataa ggttgcagac atgacaaagt ctgagttaat
     9181 ggttcgtttg gcagaagtgt ttgccgccaa aaacggcacg catcttctgg caaaagacgt
     9241 agagtacagc gtaaaagtct tggttgacac catgactaga tcgcttgccc gaggtcaacg
     9301 catcgaaatc cgcggtttcg gcagcttcga tttgaaccat cgtcctgccc gcatcggtcg
     9361 caatcccaaa accggcgagc gtgtggaagt acctgaaaaa catgtacccc acttcaagcc
     9421 cggtaaagaa ttgcgcgagc gggtcgactt ggctttaaaa gaaaatgcca attaaacctt
     9481 agcatcaaaa cgccgctgtt acgcggcgtt ttttctgtgg tttaacttca tccgttgctt
     9541 caataccttg agccaagcaa gcaacggatt agagcgtgga tttttttata gtggattaac
     9601 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acaattctct aaggtgctga
     9661 agcaccaagt gaatcggttc cgtactattt acactgtctg cggcttcgtc gccttgtcct
     9721 gatttttgtt aatccactat atcattgctt acaatccgct ttttaaacaa caaatttttg
     9781 atttctatta cgaacaggac aaaaatcctg cttattgcac taaaactaag ccgtttcagg
     9841 aatttgcggc aaatttacag cttttaccga gcctaatgct ttcgcttttt ggtaaaacgc
     9901 caatttgtat tcaagcaaat ctaaatagcg ttttaattcg gcaatttgac acttcacatt
     9961 ttctatttga ttttcaaaca aggaaaggcg ttcttcaatg gtatcgtcgc caatgacggt
    10021 acattccgca aagcgtttga tgtcttttaa gctcatcccc gtatttttca agcattgcaa
    10081 taagcccaac cattgcaaat cgttatcggt aaaacagcgg ttaccgtatt catcacgtcc
    10141 gatattgggc aacaaacctt ctttgtcgta aaaacgtagg gtgtgggcgg agatgcctat
    10201 tttttcggcg gctttggcag tagtataagt cattttccct ccttctaaac aaaaacagta
    10261 aaaaacactt gctttagagt taactctaaa gtgtaaactg ttgctatgtt gctcaggcaa
    10321 ggcaactttg tcaatgaatt aagaggaaag acaatggaaa tgaaacaagc cgattcaacc
    10381 atcaaatctc gtgcggcggt ggcattcgcc ccaaatcaac ccttacaaat tgtggaaatc
    10441 gacgtagaaa tgccgcgtaa aggcgaggtg ttaatccgca atacccacac tggcgtgtgc
    10501 catactgatg cgtttacgtt atcaggaagc gatcctgaag gcgtattccc tgtggtgctt
    10561 ggacacgaag gtgcgggtgt ggtcgttgct gtgggcgaag gtgtgtcaag cgtaaaaccc
    10621 ggtgatcacg tgattccgct ttacaccgcc gaatgtggcg aatgtgagtt ttgttgttca
    10681 ggtaaaacca acttgtgcgt ctcagtgcgt gatacacaag gtaaaggctt aatgccggac
    10741 ggcacgacgc gtttttctta tcaaggtcag ccgatctatc actatatggg ctgttcgact
    10801 ttcagtgaat actcagttgt tgcagaagtt tcactggcaa aaattaaccc ggaagcgaac
    10861 catgaacaag tatgtttgct cggctgcggc gttactacag gtattggcgc ggtgcataat
    10921 acggcaaaag tgcaagaagg cgactctgtg gcagtgtttg gcttgggagc gattggtttg
    10981 gcggttgtgc aaggtgcgcg tcaagccaaa gccggccgca ttatcgccat tgataccaat
    11041 ccctcaaaat tcgagttggc aaaacagttc ggtgcaacgg actgtttgaa cccgaacgat
    11101 tacgataaac cgatcaaaga tgtgttgtta gacatcaaca aatggggcat tgaccatacc
    11161 tttgaatgta tcggcaatgt aaacgtaatg cgtcaagcat tagaaagtgc acaccgtggt
    11221 tggggacaat ccattatcat cggcgtagca ggtgtaggac aagaaatttc aactcgtccg
    11281 ttccagttgg taacaggccg tgtgtggaaa ggcacggcat ttggtggcgt gaaaggccgc
    11341 tctgaactgc cgaaaatggt ggaagattca atgaaaggcg acattgagtt agaaccgttt
    11401 gtaacccaca caatgacact cgatcaaatc aatgaagcct ttgagttaat gcacgaaggt
    11461 aaatcgatcc gcgccgttat tcactactaa ggtatgcgat gaaactgatt gaacaacatc
    11521 aaatttttgg cggttcgcaa caagtttggg cacacaatgc ccaaacgctt caatgcgaaa
    11581 tgaaatttgc cgtctatttg ccaaataatc cagaaaatcg accgcttggt gtgatttatt
    11641 ggctttccgg cttgacgtgt acggaacaaa atttcattac caaatcaggc tttcagcgtt
    11701 atgcggcaga acatcaagta attgtggtgg cctccgatac cagccctcgc ggagagcaag
    11761 tgccgaacga tgatgcttac gatttaggac agggtgcagg cttttatttg aatgcgaccg
    11821 aacagccttg ggcggcgaat tatcaaatgt atgattacat tttgaacgag ctgccccgtc
    11881 tgattgagaa acactttcct accaacggca aacgttccat tatggggcat tcaatgggcg
    11941 gacacggcgc attggtattg gcgctgcgga atcaggaacg ttatcaaagt gtttctgcct
    12001 tttcgcctat tttatcgcca agcctcgtgc cgtggggaga aaaagccttt actgcttatt
    12061 tagggaaaga ccgtgaaaaa tggcagcaat atgatgcgaa ctcactcatt caacaaggct
    12121 ataaagtgca aggtatgcgc atcgatcaag gcttggaaga tgagtttttg ccgacacaat
    12181 tgcgtaccga agattttatc gaaacctgcc gtgcggcaaa ccagccggtc gatgtgcgtt
    12241 tccataaagg ctacgatcac agctattact tcatcgccag ttttattggc gagcatattg
    12301 cttatcacgc cgcgtttttg aagtaaacca aagagcgttc agtgttcaaa gcagttttgg
    12361 gatagccggc acgagggcgg taagaagtgc cggcataaac gtatgccgtc tgagccggaa
    12421 gaagccgact cta
//
LOCUS       DAWKOD010000048        12428 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000048 DAWKOD010000000
VERSION     DAWKOD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.048"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            555..1730
                     /locus_tag="WJK73_000991"
     CDS             555..1730
                     /locus_tag="WJK73_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ7557486.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(2071..2619)
                     /locus_tag="WJK73_000992"
     CDS             complement(2071..2619)
                     /locus_tag="WJK73_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ7557487.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(2622..4118)
                     /gene="ccoG"
                     /locus_tag="WJK73_000993"
     CDS             complement(2622..4118)
                     /gene="ccoG"
                     /locus_tag="WJK73_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ7557488.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4646..6625
                     /gene="tkt"
                     /locus_tag="WJK73_000994"
     CDS             4646..6625
                     /gene="tkt"
                     /locus_tag="WJK73_000994"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ7557489.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVTWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6766..8154
                     /gene="fumC"
                     /locus_tag="WJK73_000995"
     CDS             6766..8154
                     /gene="fumC"
                     /locus_tag="WJK73_000995"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ7557490.1"
                     /translation="MNTRTEHDTMGNVEVLSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(8219..9142)
                     /locus_tag="WJK73_000996"
     CDS             complement(8219..9142)
                     /locus_tag="WJK73_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7557491.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAVGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            9314..9454
                     /locus_tag="WJK73_000997"
     CDS             9314..9454
                     /locus_tag="WJK73_000997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557492.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(9514..10038)
                     /locus_tag="WJK73_000998"
     CDS             complement(9514..10038)
                     /locus_tag="WJK73_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ7557493.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(10042..11424)
                     /locus_tag="WJK73_000999"
     CDS             complement(10042..11424)
                     /locus_tag="WJK73_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7557494.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(11535..12158)
                     /locus_tag="WJK73_001000"
     CDS             complement(11535..12158)
                     /locus_tag="WJK73_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7557495.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            12218..>12428
                     /locus_tag="WJK73_001001"
     CDS             12218..>12428
                     /locus_tag="WJK73_001001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557496.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
BASE COUNT         3055 a         3620 c         3076 g         2677 t
ORIGIN      
        1 attcccaatc cctgagatta tccctcgggg atttcttatt acctcgacaa ctcgttacca
       61 tatctttacc ccccttcatc agactctcag acgtaatcga atcatattca aacctttgcc
      121 atgcaaaccg atatcacgta accggatgcg gtgtccgtcc aacattttac ccgattgaaa
      181 cgcctgatat attgcacccc atcaacgtgg cattactttt cttaacaatc ccctttgaca
      241 gcaactgact agggcttttt tatgccatca tcaaatttat agtggattaa ctttaaacca
      301 gtacggcgtt gcctcgcctt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
      361 ctgatttttg ttaattcact ataatatttt ctctcccgat tgaaacaggc gtaacagaat
      421 atgcccgaag ctccggctgc tttcttgttt accgccgcga tatttagagt ataataccaa
      481 atttgagcaa tagttctaaa acagttagaa ccatttttca tgagcctgac tgattcgtac
      541 actcggagaa actgatgcag aatatttttg accctttggt tattcgtgga aaatccctta
      601 cccccatcgt gcaaggcggt atgggggtcg gtgtttccgc atcgggttta tccagcgcgg
      661 tggcacgtga aaacggtatc ggaacgattg ccagtgtgga tttgcgccac cttcacgaag
      721 acctactcgc cgaatcacaa atcaatccga gtgaagagaa atatacatct ttgaactgta
      781 ccgcattaga cagggaaatc caaaaagcca aaagcgcttc agagggaaaa ggactgattg
      841 cggtcaacgt gatgaaggcg gtcaaagacc acgccgcgta tgtccgccag gcttgcgaat
      901 caggggcgga tgcggttgta atgggtgccg gtctgccttt agacctgccg gaaatgaccg
      961 agggctatca taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata
     1021 tcgtcttgaa acgttggatg aaaaaaggca tattgcccga tgcgattgta gtcgaacatc
     1081 ctgcccacgc ggccggacat ttgggtgcat caaccgttga aggcgtaaac gatgccaagt
     1141 tcgacttcaa acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta gggctggaaa
     1201 gcgaaaaaat cccgcttatt cttgcgggag gcatggcaaa ttttgaaaaa gtcaaaaccg
     1261 ccctaaagaa ctggggagca tccgccgttc aaatcggtac ggcttttgcc gttaccgaag
     1321 aaggagatgc acaccttaac ttcaaaaaaa cgctcgccgg tgcggaaact gaaaaagtag
     1381 tcgaatttat gtctgttgcc ggtttgccgg cgcgcggtgt ccgcaccaaa ttcctagaca
     1441 gctacatcaa gcgtgaaagc aaacttcaga caaacgccaa agccgacccg cgccgctgta
     1501 cccaaggttt aaactgccta accagttgcg gtctgcgcga cgggctttcc aaagccggac
     1561 aattctgcat tgatatccag cttgccgccg cattccgtgg agaagtagat aaaggcctgt
     1621 tcttcagagg taaagacccg ctgcccttcg gcaatgccat ccgcaccgtc cgcgagacga
     1681 tacaatatct gctgacgggg agcgaacctg ttgcaacgct cggacgctga catcaggtag
     1741 gatttgattt gcaaacaaaa tgccgtctga agcctttcag acggcatttt tcaggctgcg
     1801 tttcggaata gtgtcgaaaa aacggagaga aataaaaacg tttttatgta aacaaaacgc
     1861 cttccgcatt gtgtcgggca tacaacacaa atgccgtctg aagcccttca gacggcattt
     1921 ttcaggctgc atttcggaat ggtgttaaaa aataaacggg ataagataca tttattttcg
     1981 tccgataaat cagaccatca cgccgaataa tcaaacaatg cctgcccgac attgcatctg
     2041 gcaaaccaat gcaatgaaaa cacggctttt ttatttgctt ccggcattat tgaaaagttt
     2101 atccatcggg gtcagatcca ccgcattgcc ttggctggta atccatttgt tctcgacgcg
     2161 ccacacgcct gccgcgtcct ccacgcgcac ataccagtgg ttggtcggcg aaagggtttt
     2221 gaacaccgcc tcatattccg ccctgccgtt ctgcgcgctg ccgacgggct tgagggcgac
     2281 ggtttgatcg tccgccttgc gggtcgggtg catcagcagc aggttcaaag gctgtttgcc
     2341 gtcaaactcg ccgccgacaa acacttttgc cgcattcata tcgggggaaa tgagaacctg
     2401 caccccgata tgccgtctga cagcctcttc atcccgatga agctggatgt cgatatgttt
     2461 gccgtcttta taataatcgt ccgtaaccaa atctgtcgcg tgctgctgtg cgacaaaaaa
     2521 catagcgacg ctggcgatga cgacaaaaat cggccccgcc atcaagatcc acggccagac
     2581 gtgtttgtac caaggtttga ttggagtgtt ttgagacacg gttattctcc gataaaggtt
     2641 gcatcttctt ccaagacgac cggcttgccg tcgggcgcgc cgctttcgcg gtattggaag
     2701 gtaaattcga tagggtggct gcctttgtcc gcgtattccg gaatggtgga cacttggacg
     2761 ggaagggtta ccgtttcgcg cggggcaacc ttgataccgc cttcgggcag cccggtcagg
     2821 gcgatttcgt caaagccttt gacacttgcg gtaatgagct gttctttttc acttttgttg
     2881 atgatacgca ggctgtatgc gttttccagc cagcctttgg cgttttcgcg caccagtacg
     2941 ccacggtctt tcaaaatatc gacctcgacc attttgcgcg tggacaaacc ggccaggaag
     3001 gcaatgataa ctaacgccaa caccgcgccg taacctgcca cgcgcggtct gagcagccgt
     3061 tttttaatgt ctttttcaga atattcgtgt tccagcgcgc tttcggtcgt ataacggatt
     3121 aatccgcgcg gatagcccat tttgtccata atctcatcgc acgcgtcgat acaggcggcg
     3181 cagccgatac attggtattg cagaccgttg cggatgtcga tgccgacggg gcagacttgg
     3241 acgcacatcg cacagttgat gcagtcgccc aaacccgcct cttccttatt gaccgttttc
     3301 ttgcgcgcgc cgcgcggttc gccgcgttcc gcgtcataag aaacaatcag cgtgtccttg
     3361 tcgaacatcg cgctttggaa acgtgcatac ggacacatat gcaggcatac tttttcacgc
     3421 ataatgtggg cgaagaagaa ggtcataaag ccgtaaaacg ctgcggcaaa catcgcgccg
     3481 ccacctgctg ctccagtgaa taaatcggga acgaactggc ggatagggac aaaccagcct
     3541 gcaaacgtga tgcccgtcca cgcgcagaca aggaaaatca gcaggtattt ggtggctttg
     3601 atgcggattt tagtgaaatt ccacggcgat ttttccagtt tcagccgttt gtttctatcg
     3661 ccttcgacca ggttgtcaat ccacagcata atttcggtgt aaaccgtttg cgggcaggaa
     3721 tagccgcacc acagtcgccc tgcaatcgtc gtccaccaaa acagcccgaa ggcgcaaatc
     3781 atcagcagca aggcaaggta aatcaaatcg cccaccccca acgacaatcc gaaaatgaag
     3841 aaatgccgtt cggggatatt gaaaacgacg gcctgcctgc cgctccagtt gaaccacgga
     3901 atgacgtaaa acacaaactg cgtcgccaat acggcggcga tacgcagttt ggcgaaccgt
     3961 ccttccgcct ttttgggatg gatgcgttcg ccttcgggat ggatttgaat cacgctggct
     4021 cgcggatcga atgttttttt tgctttcggc gcggcttttg tttgttcgga cgtgccgatt
     4081 ccggatgccg gactgccggc ttggttttcc gtggtcattc tgcattcctt agatttttga
     4141 ttgatggttt gcccgttacc gccgccgttt gcttttcaga cgtcattttt cttgtttttt
     4201 aaggcgttgt gtttcaagtt ttgagaaaat ccgtttttcc caaaatatat ttccgctatt
     4261 gtacaacttt atgcgccgtc cggatgtatg gggcggatac atttcccatc cgcatcaaaa
     4321 cgcctggatt ttaccttacc gcccgaacaa aatccgaata cggttaaaaa aaaagactaa
     4381 aaaaaccgac acccccatat cggcagaacc gacggcgcaa gctcataaac aaacgctatc
     4441 gacaatccgg cacacaatct ataacttttt atttcaaaag gaataatggc aggcttcgcc
     4501 cgcaaatcga aaatccttcc ccgcctgtcc cctgccgccg ccttcccacg cgtccgccct
     4561 tttcttgaaa gcataagcga atcgggcgat aatcaacgct ttccgattat ccacttatct
     4621 gaaacaccag caaggaaaat acaaaatgtc tcaactggca aacgcaatcc gcttcctctc
     4681 ggccgatgcc gttcaaaaag ccaattccgg ccaccccggc gcgcctatgg gtatggcgga
     4741 aatggcggaa acattgtgga cgaaattcct caatcacaac cccgccaacc ccaaattcta
     4801 caaccgcgac cgcttcgtcc tctccaacgg ccacgcgtct atgctgttgt acagcctgct
     4861 gcacctgacc ggctacaatc tgagcattga agacttgaaa aacttccgcc agctgcacag
     4921 caaaaccccc ggccaccccg aatacggcta caccgacggc gtggaaacca cgaccggccc
     4981 gttggggcaa gggattgcca acgcggtggg tatggcattg gcagaaaaaa tccttgccgc
     5041 cgaatttaat aaagacggtt tgaacatcgt cgatcattac acctacgtct ttatgggcga
     5101 cggctgtctg atggaaggcg tatcgcacga agcctgttcg ctcgccggca ccttgggctt
     5161 gggcaaactg attgttttgt atgatgacaa caatatttcc attgatggta aagtggacgg
     5221 ctggtttacc gaaaacatcc cgcaacgctt tgaaagctac ggctggcacg tcgttcccaa
     5281 tgtaaacggt catgacaccg ccgccattca agccgccatc gaagccgcac gtgccgaaac
     5341 cggcaaaccg tccatcatct gctgcaaaac cttaatcggc aaaggcagtg ccaacaaaga
     5401 aggcagccac aaaacccacg gcgcaccttt gggcgcggac gaaatcgaag ccacgcgcaa
     5461 acatttgggc tggacttacc ccgcctttga aatcccgcaa gaaatttacg atgcgtggaa
     5521 tgccaaagaa caaggcgcga aactggaagc cgactggaac gaactgttcg cgcaatatca
     5581 agccaaatat cctgccgaag ccgcagaatt tgtgcgccgt atggataaaa agctgccgga
     5641 caatttcgat gaatacgttc aagccgcatt gaaagaagtg tgcgccaaag ccgaaaccat
     5701 cgccacccgc aaagccagcc aaaacagcat cgaaatcttg gcaaaagagc tgcccgaact
     5761 ggtgggcggc tctgccgacc tgaccccgtc caacctgacc gactggtcaa acagcgtttc
     5821 cgttacccgc gacaaaggcg gcaactacat ccactacggc gtgcgcgagt tcggtatggg
     5881 cgcgattatg aacggtttgg tattgcacgg cggcgtaaaa cccttcggcg cgactttcct
     5941 gatgttcagc gaatacgagc gcaacgccct gcgtatggct gcgttgatga aaatcaaccc
     6001 tgtatttgtg ttcacccacg attccatcgg tttgggcgaa gacggcccga cccaccagcc
     6061 gattgagcaa accgccaccc tgcgcctgat tccgaatatg gacgtatggc ggccgtgcga
     6121 caccgccgaa tccttggtca cttgggcaga agccgtcaaa gccgccgatc acccgtcctg
     6181 cctgattttc agccgtcaaa acctgaaatt ccaagcgcgc agcgagcaac aactgaacga
     6241 catcaaacgc ggcggctacg tcatcagcga agcacaaggc aacgcccaag ccgtcatcat
     6301 tgccaccggt tccgaagtcg agctggcttt ggaagcgcaa aaagccctcg ccgcgcaaaa
     6361 catcgccgtg cacgtcgttt ccatgccgtc caccaacgta ttcgaccgcc aagacaccgc
     6421 ctatcaagcc gccgtcctgc ccgaaagcct gccgcgcatc gccgtagaag ccggacacgc
     6481 cgacggctgg tacaaatacg tcggactgaa cggcgcagtc gtcggcatca accgcttcgg
     6541 cgaatccgcc cctgccgatt tactcttcaa agcattcggc tttaccgtgg acaatgtggt
     6601 cgatacagtg aaatcagtgc tgtaacccca ctcctaaaca aatgccgtct gaacgctttt
     6661 cagacggcat ttttttgccc tcccgccttg atgcttcctc attccaccaa tctccattat
     6721 aatatttgcg aatcactctt attcacattt caaaaggaga aacgtatgaa cactcgcacc
     6781 gaacacgaca ccatgggcaa tgtcgaagtc ctgtccgaag cctattgggg cgcgcagacc
     6841 cagcgcagcc gcaacaattt caaaatcggc ggcgaaaccc tgccgcagcc gctgatttat
     6901 gctttggcgt tggtgaaaaa agccgctgcc gcgaccaatg tttccctcgg caggattaag
     6961 ccggaacagg cggatttgat tacgcaggcg gcggatgatg tgttgaacgg caagctcgac
     7021 ggacagtttc ctttagtggt ttggcagacc ggctccggca cgcagtccaa tatgaacatg
     7081 aacgaagttc tggcgaaccg cgccaacgaa atcgccggta cgggtttggc ggcgtatcag
     7141 cccgtccatc ccaacgacca tgtgaaccac gcgcaatcga ccaacgacgc attcccgacc
     7201 gctatccacg ttgccgccgc gattgaaatc aaccgccacc tcatccccgc cgtaaaagcc
     7261 ctgcgcgaca ccttggacaa aaaagcccaa gccttcgccc ctatcgtcaa aatcggccgc
     7321 acccacttgc aggacgcgac gccgctgact ttaggccaag aattttccgg ctacgtttcc
     7381 cagctcgacc acggcttagg ccgtctgaac gacgcgctta aagacttgta tgaacttgct
     7441 ttgggcggta cggcggtcgg cacgggtttg aacagccatc ccgaatacgc cgaaaaagcc
     7501 gccgccaaac tcgccgaatt gtccggcttg ccgtttgtca gcgcgccgaa caaatttgaa
     7561 gccttgggcg gacgcgatgc cgccgttgcc gcttcgggcg cattgaaaac gctggcggca
     7621 agcctgaata aaatcgccaa cgacatccgc tggctggcaa gcggcccgcg ctgcggtttg
     7681 ggcgaaatca aaatccccga aaacgagccg ggttcgtcca tcatgccggg caaagtcaac
     7741 ccgacccaat gcgaagcgat gaccatggtg tgctgccaag tgttcggcaa cgacgttacc
     7801 atcggtatgg cgggcgcgtc gggcaatttc gagctgaacg tctatatgcc cgtcatcgcc
     7861 tacaacctct tgcaatccat ccgcctgttg ggcgacgcgt gcaacagctt caacgaacac
     7921 tgcgccgtcg gcattgaacc cgtaccggaa aaaatcgact atttcctgca ccattccctg
     7981 atgctcgtta ccgcgttaaa ccgcaaaatc ggttacgaaa acgccgccaa agtcgccaaa
     8041 accgcctaca aaaacaacaa atcgttgcgc gaaaccgccg ttgagttggg cttgctgacg
     8101 ggcgaagaat ttgacgaact ggtcgttcct gccgatatgg ttcatccgcg ctaatctttc
     8161 cctcaaataa aatgccgtct gaaacctcgt tcggacggca ttttccgttg cctgcgaact
     8221 agcggcgttt gaacagcctg tcccccaccg ccgccgtaac cgcacccccg accacgacca
     8281 gtgcgccggc ataacccaaa ccgttcatat ccggcgcggc aaaagtatca ggcatcacat
     8341 aatgcccgag caaagaaaat attacggtaa acacggggag caaggttgtt accgcgctga
     8401 ctttggaagc ctcccaatgt ttcaacgcct cgccgaacga gccgtaaccg attaacgtat
     8461 tcaagcagca atacgcaaaa caaacccacg ccaacgtacc gtccaaactt ccgatgtgtg
     8521 ccagttcggc aaacggcagg aacacggcgg cacttgccgc ataaatcaac agcagaatct
     8581 gttgcggccc gaattgcgcc gacagcagct tttgcgccac ggcataacac acccatgcca
     8641 tactgcctac cgcacacagc aacacgccct tcgcatacgc gcccaaaccc gacaactcgc
     8701 cgaatttatc gttaaaaaac ataagcaaac cggcaagcag caaaaccaag ccgattttct
     8761 gagcggcagt catccggtct ttaaacacca acacaccgac aacaatcatc gtaaacggcg
     8821 aaatctgcca caaaacctgc gtcgtggtcg gcgaaatata atgcagccct tgggcaatca
     8881 gcacaaagtt tgccgaaatg cccgccacgc cgagcagcag cagcctgaat gagcaccaag
     8941 aaaaatcccg ccacttcggc agccgcccgc ccaatgccag caaaacaaac aataccgccg
     9001 ccgccacggt aaaacgcacc cacaccagcg tcggcgcatc gacaaacttc aatacctgcc
     9061 gcacggcaat cggcagcgtt ccccacgtca tcgccgccaa aagtgccaac gcgaagccta
     9121 ggagcggcct ttggttttcc atcctgattt tcctattttt aaacaaccgt attgccggac
     9181 gatgccggtt tgccgcatcg ggcaatgatg gttcaagcgt ttgacgtttg attccaaccc
     9241 tttgatttca aacaaaccgg ctgaagcccg actattgctt cgcgctattt ggaaacaccg
     9301 cctgaatttt aaaatgccgt ctgaaaacag cggggacgtg cggcaggcat cactgcctca
     9361 aaacgcccga acggcgcaat cgcaacgttc cgcccaacgc aaggggcggc aacaagccgg
     9421 ctcggatgca aaaaagagaa accctgcccc gtaaggttta aggtttctcc gtcctttatg
     9481 atttccctct gcgaggatgt ccggccgtaa aattcagaac gggatgtcgt cgtcaatatc
     9541 ctcgaccggg gcggcggcag gcacgggttg gcggcgcggc gcggctggtg cttcttgggg
     9601 atgggacggc gcgtcggagg cgggctgccg gctttgctgt gcggggcgtt ggtaagcctc
     9661 ctgactctga ccgtaacctt cctcgtaagg cgcaccgccg ctgttttcat tgcgcccgcc
     9721 caacattttc atttcgttgg cgacaatatc gtaagcggtg cgttcgatgc cgtctttgcc
     9781 ttggtatttg cggctttgga ttctgccttc cagataaacc agcccgcctt ttttgaggta
     9841 ttgcccggca atttccgcca gtttgcggta catcgtgatg ttgtgccact cagtacgctc
     9901 tacacgttgg ccgttgcggt cgttccaagt ttcgctggtg gcgacgctga aattacacac
     9961 cgcctcgccg ttgggcatat agcgcacttc gggatcgcgt ccgaggcggc cgataaggat
    10021 aactttgttc aatgacattt tttaaactcc tgtgatgatt ttttcagcgg cagcctgatc
    10081 gaaacccttc tgcaacactt tgagatagac ggtctgcccg tcgaaactga aaccgatgtc
    10141 ttccacaccc tcaagctccg acaaggcgcg gtataacact tcctgattga cctgccacac
    10201 gccgccgaca gggtaactga ggtttttgac gggcttgggc gcaggcgata aaacggcaat
    10261 caccagccac agcagcatca atatgctgca aaaggcaaac acgccggaaa agccgtattt
    10321 ttgaaacagc aaaccgcctg ccacgccgcc ggcaaacagt ccgagcgact gcatcgtgtt
    10381 gtacacgccc atcgccgtac ccttcaggtc ggacggcgcg attttggaaa ccatagacgg
    10441 caggctcgct tccaacacat taaaaccgat aaagtaaaca accaaataag cggtaatcaa
    10501 gcctaccgag cgcataccgg acagcaaacc gagctgtgcc gccgcgatgc aggcgatgcc
    10561 caagataaaa acctgtttga gcttgttgcg cgtctcgccg acgataatca gcggaaccat
    10621 caccaccaag cccgtaatgg tcgagggcag atagactttc caatgctgga ttttttccaa
    10681 accgagctgc gtcatcgcaa aaggcagcgc ggtaaacaat gccatttgcg cggcgtgcag
    10741 ggcgaaaatg ccgaaatcga gcgtcagcag cctacggttt ttcaaaactt cgcctatgcg
    10801 cgaaggctgc gcctgcgtat cttcgtgcag cttggaaacc tccgggtcgg gagtcatcca
    10861 cgccaccacg ccgatactgg caacggtcaa aataccggtc agcagaaaca agccgcgtac
    10921 gcccaccgcg tcggcaatca caggggcaac gacgaggctg accgaaaacg tcagaccgat
    10981 actcaaaccg atcatcgcca tcgcgcgggt acgtacgccg tcgcgcgtca aatccgccag
    11041 cagcgcggta accgccgcac tgaccgctcc cgcaccctgt atggcgcgtg cggcgaccag
    11101 catgggcagc gtatcggcgg cggcggcaag gaagctgccc gctgcaaaca caatcagccc
    11161 gacataaatg gttttcttgc gcccgaactt gtcggaagcg atgcccaaag gcagttgcag
    11221 cagagcctgt gtcagcccgt aaatgcccat tgccagcccg accagcgttt tgttgccttc
    11281 cgcgccgggc agcgaggcgg catacaccgc caatacgggc agcacgagga acatacccag
    11341 catacgcagc gcgtacacgc cggaaagcgt cgtactggcg cgccattcgt gcggaaacat
    11401 ttggatgcgg ttatctcttg ccatcatatt ttttcagacg gcatcaacag ttgcaatgcc
    11461 gtctgaactt ccagtgaaca gattttcgga ttatacagga ttcgccgtat ttcggttgcg
    11521 gcgcgggttc aaaatcaacg ccactgccag cggttgcgcc acgcgcccaa aacggcgttc
    11581 ggatatttat tgctgcccaa gctgccgtta aagcgggcaa gcgcgcggac gatgttgcct
    11641 ttttcaagat tccggtaatg gcgcaggatg gtacagccgt aacgcaggtt ggtgcggatg
    11701 tcgaacaggt tgtgcgccgg tttgccgatg tagtttttcc aaaacggcat aacctgcatc
    11761 aggccgcgcg cgccgacacc gctgattgca tactggcgga acgcgctttc cacctcaatc
    11821 agccccaaca caatctgcgt atccaaaccg gcccggctgc tttcgtactg gatattgacc
    11881 agcagcctgc gccgctcctc ctcctcgggg acgaaccttg ccaaacgtgc cgacatggca
    11941 gacaaccaac gttcgccctc tttcggattg tcaaacacca gcctcggcgg attgacgctg
    12001 ccgacagaac tcctcatcac ggaagccaca tcgtcggcaa gcgtttcctc acgttgcgcg
    12061 ccggcgtgcg ccagaggact gagcaacaac gcaccggcgg cacacaacag gcggcggcgt
    12121 tgcagattaa cgggtagggt atcggtcggt tttttcatag ggaacggggg cgcgtccgga
    12181 cgtttcagac ggcattaaat attcaaacag acaatgattg cttccaacgc gaaaaaccgc
    12241 gcgcaaaatc caagcgcggc atatcgccct gcccttttcg ggcaaacctc aattctaccg
    12301 ccctcaagaa cgcttgtcca aacaggcaca ggcaacaccg cccgggcatt tccgttttca
    12361 ccggttatcc gtcgtccgga ttatgcagca gcaccatcag cgcatcacgc ttttcgggcg
    12421 gcagcagg
//
LOCUS       DAWKOD010000049        12019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000049 DAWKOD010000000
VERSION     DAWKOD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.049"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            269..1453
                     /gene="coaBC"
                     /locus_tag="WJK73_001002"
     CDS             269..1453
                     /gene="coaBC"
                     /locus_tag="WJK73_001002"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ7557497.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIYGQLQTELPFGISNRVQTVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1520..3676)
                     /locus_tag="WJK73_001003"
     CDS             complement(1520..3676)
                     /locus_tag="WJK73_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7557498.1"
                     /translation="MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLAVWHMDVQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3769..3975)
                     /locus_tag="WJK73_001004"
     CDS             complement(3769..3975)
                     /locus_tag="WJK73_001004"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ7557499.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDARNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(4034..4651)
                     /gene="gmk"
                     /locus_tag="WJK73_001005"
     CDS             complement(4034..4651)
                     /gene="gmk"
                     /locus_tag="WJK73_001005"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ7557500.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLVHIVNACRLKRSRQLGFIADLLENS"
     gene            4779..5378
                     /locus_tag="WJK73_001006"
     CDS             4779..5378
                     /locus_tag="WJK73_001006"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ7557501.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKINIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5424..6212)
                     /locus_tag="WJK73_001007"
     CDS             complement(5424..6212)
                     /locus_tag="WJK73_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ7557502.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMVETGMDAVVTINGQFALLHGKTVHEVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVALGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6537..7892
                     /gene="yegQ"
                     /locus_tag="WJK73_001008"
     CDS             6537..7892
                     /gene="yegQ"
                     /locus_tag="WJK73_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ7557503.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATENDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            8026..8502
                     /locus_tag="WJK73_001009"
     CDS             8026..8502
                     /locus_tag="WJK73_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ7557504.1"
                     /translation="MLAVCQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8755..9096)
                     /locus_tag="WJK73_001010"
     CDS             complement(8755..9096)
                     /locus_tag="WJK73_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7557505.1"
                     /translation="MPQVKLIITRPAQSNLVRIYNFLNDLGAEHQANTVMRLLKNSFV
                     STQGSPSNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFR
                     ENAYRLSFPFL"
     gene            complement(9084..9371)
                     /locus_tag="WJK73_001011"
     CDS             complement(9084..9371)
                     /locus_tag="WJK73_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557506.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVDGLGGDESKDMPECRK"
     gene            9441..9551
                     /locus_tag="WJK73_001012"
                     /pseudo
     CDS             9441..9551
                     /locus_tag="WJK73_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(9741..11200)
                     /locus_tag="WJK73_001013"
                     /pseudo
     CDS             complement(9741..11200)
                     /locus_tag="WJK73_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(11331..11960)
                     /locus_tag="WJK73_001014"
     CDS             complement(11331..11960)
                     /locus_tag="WJK73_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ7557507.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
BASE COUNT         2943 a         3269 c         2781 g         3026 t
ORIGIN      
        1 cacagggaaa aacggtcaat gctttcagtg ggattttttt ggggaaattc gtcatgtcgc
       61 tgtcggataa ggttttttta ttttcgccaa acgctgcacc acctccaaca atcccttcct
      121 ccccctcctc tgactggtgc gcctttgtga atatgccgtc tgaaactcgg ggactcagac
      181 ggcattttgt atccaaacgg tatctaatgt atccacactt tgttatagaa tggctgctgt
      241 tttttcttcg taattagaaa ttgtcaaaat gggcaaacat attcttttag gtgtaacggg
      301 cagtattgcg gcgtataagt cttgcgagtt ggttcgtctg ctgaaaaaac aggggcattc
      361 ggttacggtg gttatgagcc gctcggcaac tgaatttgtt tctccgctga cttttcaggc
      421 tttaagcggc aatcctgtcc tgaccgacac gcacggcggc aacggttcaa acggtatgga
      481 acatatcaac ctgacccgga atgcggatgt ttttctgatt gcgccggcaa gtatgaatac
      541 cgtggcaaaa atctgtaacg gcgtggcaga taacctactg accagtctgg cagccgcacg
      601 gaaatgtcct cttgccatcg ctcccgcgat gaatgtggaa atgtggctca accctgccaa
      661 ccaacggaat atcgcacaac tggtttcaga cggcattact gtctatatgc cgggcttggg
      721 cgaacaggct tgcggagaaa atggtatggg aaggatgccg gaacctgccg aattgctgga
      781 tttgcttcca gacttgtgga caccgaaaat tttaagggac aagaaaatct tgattaccgc
      841 aggtgcgaca tttgaagcca ttgaccctgt caggggcatc acaaatattt ccagcgggaa
      901 aatgggcgta gctttggcgc gggcgtgtcg tgccgccggt gcggaagtca gcctgattta
      961 cgggcaactt caaaccgaac tgcctttcgg tatatccaat agggttcaaa ccgtcagtgc
     1021 tgaagatatg catcgtgcag tgcatcgttt aatcgacaaa caagatgctt ttatttctgt
     1081 tgccgccgtc tcagactata aggttaaaaa caggagtacg caaaaattca aaaaagataa
     1141 aaatgccaaa ccattatcca tcgaattgga tgagaacccc gatattttgg cttctattgc
     1201 ctcattgccg aacccgccgt tctgcatcgg ttttgccgct gaaacggaga atgtaatggc
     1261 atatgcgcga gaaaaacgta ttaagaaaaa aataccagtg atcgttgcca atgatgtttc
     1321 aatcgcgatg ggcaaaccga ccaaccggat taccattatc ggggacgacg aggaactgtc
     1381 ttttcccgaa acaagtaaag atgaagcggc aatgcagatt gttgaaaggc ttgccgtata
     1441 tttgaacaaa taagcaattg aacggataaa ccataaagcg ggttgcctgt taatcaaaag
     1501 gcaacccgtt ttacctgctt caacttctga tgactttgcg gatatatgga atactatgca
     1561 gattttgaat aatctgattc aattgattca gattcttgac tttcaataag aatttgaatt
     1621 cgacaaaacc ttccgttccc gactgggatt tagacggtgt ttcgaccgac tcaatgtccg
     1681 caccggaatc ggaaatcgct tgcgccatta atgccagcaa gccgtggctg tcttccgatt
     1741 ggacttgaag cccgacacgg tagttctgcc cgttcatatt ttcccagtct gcatccagct
     1801 gctgttcggg atcggacttt aacaacgtcg ggcaggtatc cctatggata atcatgcctt
     1861 ttcccttaac caacagcaaa cggatggaat cgccgggaac cggatggcag cactctgcaa
     1921 aatgaatatg cccgctttcc tgaccatcaa ctttaatgga actgagcctg acctcgctgc
     1981 cgaaatgctc ccctgccaac tcggcaatgt gcatggcgac ataaacaggc agggtatgcc
     2041 ccataccgac gttgtacagc acttcctcaa atgaagtctg tttgtcgttg agatcggcaa
     2101 ggtatttttc tttaatgccg tctgaaagca ggacatcttt gggcagcaaa ctggacaggg
     2161 ctttttgtaa gaggctctct cccaaaacga ccgcatcgtg ccggttaagg tttttaatat
     2221 attggcgtat ggcactgcgc gccctgcctg acacggcgaa attcaaccat gcaggattgg
     2281 gtttggcgtg ttcggatgtg ataatttcaa cagaatcacc ggttttgagc ttcgtacgca
     2341 acggcatcat gatattgttg atacgtgcgg caacggtttt gtgcccgata tcggtatgca
     2401 ccgcataagc aaaatcgaca ggcgttgccc ctttgggcaa agttaggatt tttccttttg
     2461 gcgtaaggat gtagatttcg ttcggaaaca aatcgacttt gacgtgttcg agaaactcaa
     2521 tggcattggc actgcttgcc tgcaaatcta agatattttt cagccaccgg tttgtgtgaa
     2581 gcaccgcctg atcgaccgtc ttagaatatg atttatagct ccaatgtccg gcgattccac
     2641 cttcggcaac agcatccatt tccttggtac gtatctgaac ttcaatcggc aagccgtaag
     2701 ggccgaccaa agtcgtatgc agactttgat acccgttgct tttcggaatg gcgatatagt
     2761 ctttgaaccg cccgggcttg ggctgataga gggtgtgcaa tgcaccgagt gcggcataac
     2821 aggctggaat gctgttgaca atgacgcgga aaccgtaaat atccataacc tcggcaaagc
     2881 gcagcttttt cgccatcatt ttctgatgga tgccgtacag gtttttttcc ctgcctttga
     2941 ttttggcctc tatattcgcg cctaccagcc gctggccgaa tgcgcgcaag actttgccga
     3001 caacgtcctg ccggttcttc cggctcttgt ccatcgcttt ttttaaagtc tcgtagcggt
     3061 tgggatgcag gttttggaac gataaatcct gaagctcttg atatgcgtta ttcaaaccta
     3121 tacggttggc aatctgtgca tagatttcaa gggtttccct tgcaatccgg cggcgtttgt
     3181 ccgggcgcat cgaaccgagc gtccgcatat tgtgcaggcg gtcggcaagt ttgacgacga
     3241 tgacgcgcac atctttggtc atcgccaaaa tcagtttgcg gaaactctcc gcctgatgtt
     3301 ccgcatgatc ttcaaatttg agtttttcga gtttggacag cccgtccacc atctcggcaa
     3361 tcgtattgcc gaacaccgcc gccatttccc cttttgtcac gcccgtatct tccaatacgt
     3421 cgtgcattac acctgcacaa agaccctgta cgtccatatg ccaaaccgca agctgtgtcg
     3481 caacggcaat cggatgcgta atgtatggtt cgccactttt gcgggtttgc ccgtcgtggg
     3541 cgcgaaacgc ataggcgacg gctttttcaa gctccgcctg ttcctcgggc ttgaggtagg
     3601 aggctgtatg gaaaagcagg gtacgcgctt cggcggtcag agggtcgtaa ggtgcggtgg
     3661 gttgagggac aggcatttca gacggctttc ggaacatatt ttatttttcg tttcaaaccg
     3721 tcggactgca cggcggcaga atgtgccgac gtgcgtttcc ggcagaattt atttattgcg
     3781 cgtcaacagt tccgtaccga tatgcccggc ggcgatttcc cttaaggcgg taacggtcgg
     3841 tttgttattg cgggcatcgt ccacaagcgg cgtgttgccg ttctcaagct ggcgggcgcg
     3901 gcgggccgct accaatgtca ggtcaaaatg gttggaaatt tttccggtac agtcttcggt
     3961 ggtaatacgt gccatattat ttgctttctt tcaaaaatat ttaaattggg aaaccgggta
     4021 ttttcgccgt tttctaggaa ttttccaaca aatctgcaat aaatcccagt tgccgcgacc
     4081 ttttcagacg gcaggcattc acaatatgaa ccaaatcctc ctccgcccgc gccaagtcgt
     4141 cattgaccac gacaaagtcg aacaatacgg actgttcgat ttcatgcctt gccttcgcaa
     4201 gcctcctttg gataacttcc cgactgtccg tcccgcgtcc gttgagacgc gcggcaagca
     4261 catcgaaaga aggcggcagg ataaagatac cgacggcttc gggcagcgca tctcgaacct
     4321 gcgccgcgcc ctgaacgtcg atttccaaaa tcacgtcata acctgccgcc gccaacgcat
     4381 tcacgccctc cgcgcttgtg ccgtaatagt tgccgaatac gtctgcgtat tccaaaaaag
     4441 cttcctgcgc gataagcgac tcaaactctt ctttggaaac aaagtgataa tgtacgccgt
     4501 ttgcttcgcc ttcacgcggc gggcgcgtcg tatgcgatac ggaaacgcgc aaaccgttat
     4561 ggtttgccaa cagccgcgac accagcgtgg ttttgcccgt gccggaagcg gccgaaatga
     4621 taaagatgtt gccttttcga taagcggaca tattttttac ctgtatattt tccagccgat
     4681 tgtatcacaa cggacaccca gtttcctatt tgccgatgcc catattttgc cgctattgtt
     4741 ttgattgatt tggcaagcgg caggctgacg gctacaatat ggcgttaaaa acatcaaact
     4801 tggaacacgc aatgctggtt catcccgaag ctatgagtgt cggcgcgctt gccgacaaaa
     4861 tccgcaaaat cgaaaactgg ccgcaaaaag gcatcttatt ccacgacatc acgcccgtcc
     4921 tgcaaagtgc ggaatacttc cgacttttgg ttgatttatt ggtttaccgc tatatggatc
     4981 agaaaatcaa catcgttgcc ggtttggacg cgcgcggctt cattatcggc gcggcactcg
     5041 cctaccatct caacgtcggt tttgtcccca tccgcaaaaa aggcaagctg ccttttgaaa
     5101 ccgtatcgca aagctacgcg ctcgaatacg gggaagctgc ggtggaaatc cacaccgatg
     5161 ccgtcaaacc cggttcgcgc gtgctgctgg tcgatgattt ggttgccacg ggcggcacaa
     5221 tgcttgccgg gctggaactg atccgcaaac tcggcggaga aattgtcgaa gctgccgcta
     5281 ttttggaatt taccgacctt caaggcggca agaatatccg tgcaagcggc gcgcccttat
     5341 ttaccctgct tcaaaacgaa ggctgtatga agggctgaaa accgaccctg ccgtctgaaa
     5401 ccggcagggt tgttattatg cgttcaaatc acgcccaaat cttgcaagcc cctcaacacg
     5461 ccgtcttcat caacgctggg gcaaacatat ttcgccgctt ctttcgccgc ctgttccccg
     5521 ttgcccagtg ccacgccgaa cccgacttca gacagcattt ccacatcgtt caaaccgtct
     5581 ccgaacgcca tcacgtccgc catttccaaa cccaatgctt caaccacgct tctgatgccg
     5641 tctgttttcg acattcccgc aggcagcaga tcgaccgctt cctcgtgcca gcgcaccgtt
     5701 ttcaagcctt cctgttccac aatatccgac cagagcggca tttcgttttc ctccgcaaac
     5761 accagcatct gatacaccgg tttgcttgaa aaataatcct tatcggcaaa aaaatcgctg
     5821 gcgatatgct gcaaggcgcg gcacacgcat tccgacagcg cggacacggc gatcccctct
     5881 ccgccgacaa acgcataatc catgcccaag ccatccaaat gcgcgcaaac cctgcccatc
     5941 aaaccggcat ccatcggtac ttcgtgcaca gtttttccgt gcagcagcgc aaactgtccg
     6001 tttatcgtta ccacggcatc catacccgtt tccaccatca tgtccctgac cttttcggga
     6061 atcgtcgcca aagaccgccc cgttgccaac gccgtcaata tacctttgcc gcgcaaagcc
     6121 gccaccgccg ttttcacgga aggacgcaag gtatccgtat atttgcggta cagcgtatcg
     6181 tcaatatcga aaaacacgat tttgggattc atcacattct ctctcgcatt caaattgccg
     6241 cattgtatcc caagcaggca aatacttgat aaatccttat aaatttcccg gcaaaattga
     6301 ccgcgaacac gaaaaggcgg ataatccgcc catcctcaaa cccttttcag acggcatttg
     6361 cagcaatgcc gtctgaaaca tttttacaaa gcacacaaat catgtttcaa cacacaggac
     6421 gacacataaa gcgtcgccct atatgttgcc ctgattcgga aagggttaca cccctcccaa
     6481 ataaagtctg attctaccgc cctaaagggc ggggtttcaa ccgaaaagga aacacgatga
     6541 aagcacccga actcttattg cccgccggcg gattggaaag aatgcgcacc gcctacgact
     6601 acggcgcaga tgccgtttat gccggcagcc cgcgttactc actgcgcgcc cgcaacaacg
     6661 aatttgccaa actcgacgtt ctcgagcaag gcattaaaga agcgcacgag cgcaacaaaa
     6721 aattcttttt gaccgtcaac accctgccgc acaactccaa actcaaaacc ttcgtttccg
     6781 atatggagcc gctgattgcc atgaaacccg acgcgctgat tatggcggat ccgggtttga
     6841 ttatgaccgt gcgcgaaaaa tggccggaaa tgccgatcca cttgtccgta caggccaaca
     6901 ccaccaacta ctggggcgtg aaattctggc agaatatcgg cgtcgaacgc atcatcctgt
     6961 cgcgcgaatt gagtatggaa gaaatcgccg aaatccgcca acaatgcccc gacatcgaac
     7021 tcgaagtctt cattcacggc gcattgtgca tcgcctactc cggccgttgc ctactctccg
     7081 gctacttcaa ccaccgcgac cccaaccaag gcacctgcac caactcctgc cgttgggact
     7141 acaaagtcca caacgctacg gaaaacgatg cgggcgatgc ccagcttctg caaggtttta
     7201 actttgaaaa agcccaagaa gaagccaacc aaaactttga aggcatcaac ggtcaaaaac
     7261 gccatcccta cgccgacaaa gttttcctga ttgaagagtc caaccgcccg ggcgagatga
     7321 tgccgattat ggaagacgaa cacggcactt acatcatgaa ctccaaagac ctgcgcggca
     7381 ttgaagtggt tgagaaactc gccaaaatcg gcgtggacag cctcaaagtc gaaggccgca
     7441 ccaagtcgct ctattatgtc gcacgcgtcg cccaatccta ccgcaaagcg attgacgatg
     7501 ccgtcgcagg ccgtccgttt gattacagcc tgttgagcga actcgaaggc cttgccaacc
     7561 gcggctacac cagcggcttc ctcgaacgcc accaaaccca agactatcaa aactacctca
     7621 gcggccattc caccgccaaa caaagccaat acgtcggaca cgttaccgaa atcgacaaaa
     7681 acggctgggc gaccatcgaa gttaaaaacc gcttcgccgt cggcgattca ctcgaaatca
     7741 tccacccgaa cggcaaccaa accatcaaat tggaacaaat ggcccgaaaa ggccagcctg
     7801 tcgatgtcgc acccggcaac ggcattcagg tcaaaatccc cgatatgcag ggcaaagaaa
     7861 aagccctcat cgcacgcgtg ttgaacccct gagccacaat gccgtctgaa ccccgttttc
     7921 agacggcatt ttgttgaatc ttacggtaca atggcaatga tttcccaccg caagcaagga
     7981 tatccgacat gaaaaaaaat cctcatactc tccgccgccg ccctgatgct ggcagtctgc
     8041 caatccggca gcgcacctaa aaacgcgcac acggcatccc ccggcacacc ggccggagaa
     8101 tcatgcaaag tcatccgggg cgacaccgca ggaagcggca aaaacatcat ttaccgttgc
     8161 tgcaacggtc aggcggcaat aaatgcagcc attgcagacg gcgtattgga atcagggatt
     8221 cccgtcagct tcggcggcgg gggcgtaccg gttgcaaacg gcaggaatct agttacccgt
     8281 caggcggcaa acggcatagg caaatccgac tcagaagcct acgaacgcgc cctaatcaat
     8341 gccgcccgca aattccaaca gaccgcaggc aaactcggcg gacgcggcgt taccggcttc
     8401 cacagctact ttgacaagca accgctgcaa ggcgggcaat acgactgtca ggcaggctcg
     8461 ttccacgtcc gcgtcgtcat gcgcgccaat gtcgtccgtt aacatatcgg caatcaaaaa
     8521 atgccgtctg aaacattttt cagacggcat ttttaatccc cttgccttat tgtgcggcag
     8581 attcagatcg ggacactcca tacttccata tctgcaccgt tcaaacatcc cgcctgcgga
     8641 acggaatcaa agcggcagtc cggttatgtt cttgaaggcg gtttatccga acgtgccgca
     8701 acaaagccga aacgcattgc cccgcacaag tgaaagccgc caaaccaaat accgttataa
     8761 aaaaggaaag ctcaggcggt aggcattttc acggaaatgc ctcaccgtca aaatcaatat
     8821 ttgcttacct gatttatcgt gccaaaacaa atagctataa ccgcttttgc cataaggcac
     8881 gattacttcc cttacatttt gcaaagtttc ccccgccacc gacaaatcat aaggtttccc
     8941 attactcggg ctgccttgtg tagaaacaaa agaattttta agcagccgca tcactgtatt
     9001 tgcctgatgt tccgcaccca aatcattcaa aaaattataa atcctgacca aattagattg
     9061 agcggggcgt gtaattatca gctttacttg cggcattcgg gcatatcctt cgattcatcg
     9121 ccacccaagc cgtccaccca gtctgcaaac tcattaagcg taatatgcaa tcctgtttcc
     9181 cgataatcct gaaaagcctg catcgcccgc ttatcgtagg cttgtttggc tttcaagcgt
     9241 tctatatgac catccaccgc atccaatacg atactgtgca cactgccccc gaaatcccgg
     9301 gccagctctt gtaaaacggc tttgcggtat cccagtttga caccggttac ttcctgattg
     9361 gtaagcatca ttattttccc ataaatcaaa cgtctgacac ggcattataa acacaatgcg
     9421 gcatctgccg ccacccttgc ggacgcggcg ttaccggctt cctcagctat ttcgacaagc
     9481 agccgctgca aggcggacaa tacgactgtc aggcaggctc gttccacgtc cgcgtggtca
     9541 tcgtccgtta aacatatcgg caatcaaaaa atgccgtctg aaacattttt cagacggcat
     9601 agttcataaa tcaaatcggt aatacagata atcgttcaat taggattgct tctgcaggtg
     9661 ggacaacaca tcaaggtgtt gcccgatgcc ctgtcggaag gaaacgccac tgttcccgac
     9721 tgtgaaacac aggataaata tcaacggacg tttgcttctt ttttgcctgc aaatacgccg
     9781 aagccgttgt cgctaaaacc atcattttta tcaggggttt tattgaaact gtatctgccc
     9841 gccatttcat ctccattaga gccataaaac ttcccttcaa catctccttg ttcttttgta
     9901 tcccatttgc ctttaaagcc acttccctca atcttggcag taaattccct tgttcccata
     9961 tacaaaccat cgccactatt gattgtccct tttacagact tctgaccgaa atcgacttct
    10021 gctgcaaacg tacctgtcgt cctatatgaa tttccttcct ccaaccagtc tgtttcatca
    10081 gtaacattgc gggaacggaa atgcagcacc ttaccttgat atttagcttt gccttccggc
    10141 agattatttt ctgtcggctg accttgtaca aaaagagtat atgagccatc ttttaattta
    10201 acagccccat aaatcagatt cttatattgg ttttgagaaa ccattacctt atcaaaatca
    10261 ctcatcaaac cggggtgatt tctacttact ggctcattag ctttcgttaa atcaatagtt
    10321 tctccgccaa tcaccaacgc cgtgatttct cctgtgccgt caaaatattt ctcgccaaac
    10381 acgcctgttt taggttgagt atcggcagca ctttggactt gtttgtcttg ggaatccgga
    10441 ttttcactta caaacttttc ctccgtatca tcttgagatt tcgcaacttt ctcgacatct
    10501 tgattgccgc catccgaccc tacggcatca ctgccgggca tatcacggtt gttttcaggc
    10561 tgcttgccgt cggcagcact ttgggcttgt ttgtctgggg aagacggagt tacgcttaca
    10621 gcctttccct ccgtatcatc ttgagatttc gcaactttct cgacatcttg attgccgcca
    10681 tccgacccta cgggatcact gtcgggcata cgttcttttt caggctgctt gccgtcggca
    10741 gcactttggg cttgtttgtc tggggaagac ggagtttcgc ttacagcctt tccctccgta
    10801 ccatcttgag atttcgcatc tttctcgaca tcttgattgc cgccatccga ccctacggca
    10861 tcactgtcgg gcatacgttc tttttcaggc tgcgtgtcgg atgtgccgtt tgccggcaca
    10921 ttatcattat gttggggcgc atcattttgc actccgccat cagcgcctgc ctgcgcggtt
    10981 tccccttctc cctgcgtatc tttagacggt atttctgttt tggattcccc tccgtccccg
    11041 cccgaggtag gatttaccgt ttcagcgggg ggggggtaac tttatttgac ggcgtttctg
    11101 aggatttgac tacaggtgta ccccctcccc ctgaaataca ggcggaaagg gcgaggatgg
    11161 atgccattgc aagcaggctc ggtttgaaag gaatcgtcat aattatctcc tatggcatat
    11221 ttttaatgaa aaatatgata attatttatg tttaatggtt taaaaatacc gccatacgcg
    11281 gcaggaaata cggctctgct tcacaaatcg gggcagggtc gtaaaacaca ttagtgcctg
    11341 tgcggcatca ttccttccgg tgcttcggca tcggctgcca agccgaaggt ttcgcgcaac
    11401 acgactttgt agaatgcaaa ggcttcgcgc gcgccttgga tggcttcggc ttcggcttcg
    11461 gggcttaagc ccaatgcgtt cagatgctcg acgaaggcgc gccagtgttt gccgcgcccg
    11521 tcgggatgcg gagcgaggtg gcgcgcgccg tgttcgccgt tgtaatcgag tttttgggcg
    11581 tgtttgaaca aaaacgccgc gcccaaattg gatccttcgg cgcaataaag ccagccgatt
    11641 gctttgttgc cagtttcgta tggcaattct ttgtcgaatt tgtaaggctc ttcgcccaaa
    11701 tctttcaagt cttgcgttac ggcatcgtat cgcgccatgt attccagctc gggaatggct
    11761 ttgtttaatt cggcatcttt atagatgtgg tcgacggcct tgtggaaaac ggattggagt
    11821 ttcaaaaatt tgatgtagtt ttctttgctg acaaacggtt ggacagacat aacgaggtta
    11881 tccacgctgt cgtgaaccgc cgtggtatcc gccttcagac gtttggcaaa cgtcagggct
    11941 tgattttcgg tttcactcat aagttttcct ttgtcggtaa gggataagga tgcggggcgg
    12001 gcaaaatccg cacttgccc
//
LOCUS       DAWKOD010000050        11609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000050 DAWKOD010000000
VERSION     DAWKOD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.050"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..466)
                     /locus_tag="WJK73_001015"
     CDS             complement(131..466)
                     /locus_tag="WJK73_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7557508.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            595..1500
                     /gene="mtrA"
                     /locus_tag="WJK73_001016"
     CDS             595..1500
                     /gene="mtrA"
                     /locus_tag="WJK73_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ7557509.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1562..2050)
                     /locus_tag="WJK73_001017"
     CDS             complement(1562..2050)
                     /locus_tag="WJK73_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7557510.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2197..3099
                     /locus_tag="WJK73_001018"
     CDS             2197..3099
                     /locus_tag="WJK73_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7557511.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3140..4270)
                     /locus_tag="WJK73_001019"
     CDS             complement(3140..4270)
                     /locus_tag="WJK73_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7557512.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPSHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4475..7099
                     /gene="alaS"
                     /locus_tag="WJK73_001020"
     CDS             4475..7099
                     /gene="alaS"
                     /locus_tag="WJK73_001020"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ7557513.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTNLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7161..7892
                     /locus_tag="WJK73_001021"
     CDS             7161..7892
                     /locus_tag="WJK73_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557514.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8601..11012
                     /locus_tag="WJK73_001022"
     CDS             8601..11012
                     /locus_tag="WJK73_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7557515.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAHRTFQWTSQAAGKAAVH
                     CIIVGFRQKPPMPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPIHCE
                     PDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLWFHG
                     VSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGNYLI
                     IPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAGRLK
                     SDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQEAG
                     LPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFELYRK
                     AAAIA"
BASE COUNT         2965 a         3209 c         2741 g         2694 t
ORIGIN      
        1 acgcttaccc cttgtttctc aaatgccgtc tgaaataagc ggcttaatat attgtttaca
       61 gtattgggaa gcataacaga caaaatgccg tctgaaatat tttcagacgg catttcttat
      121 ccgaaacgga ttatttttgc gtttcaaccg cttccaatgc acgcagggcg taagtgtaag
      181 cggcaccggc attcagggcg atggcggttg ccaatgcgcc tgcgatttcg ctgtcggtcg
      241 caccggcttt ggcggcagcg gcggcgtgaa cgctgatgca gctctcgcaa cgagtggtaa
      301 tggcaacggc gatggcaatc agttcgcgcg ttttggcatc aagtgcttcg gcggcggcgg
      361 cctgttccaa tgcgccgtag gcctgcagca ttttaggatg cgccttaccc agctcgccga
      421 acgatttttt aaccaatgcg gtatgttctt tccaatcttt aaacattttc ttttcctttc
      481 tcttgcgttt aaccctgata cgcgcttgcg tatctgtttt cgatgtgcgt attattgcaa
      541 ttattcagtt gtgtttctcg tttaatcatc tcattttatg gttcaaaaag atttatggac
      601 attctggaca aactggtcga tttcgcccaa ttgacgggca gtgtggatgt gcagtgcctt
      661 ttgggcggac aatggtcggt acggcatgaa accttgcaac gcgaaggatt ggtacacatt
      721 gttacatcgg gcagcggcta tctctgcatc gacggcgaaa cttccccgcg tccggtcagt
      781 acaggggata ttgtattttt cccgcgcggc ttgggtcatg tgttgagcca cgacggaaaa
      841 tgcggagaaa gtttacaacc ggatatgcgg cagcacggtg cgtttacggt caagcagtgc
      901 ggcaacggac aggatatgag cctgttttgc gcccgtttcc gctacgacac ccacgccgat
      961 ttgatgaacg ggctgcctga aaccgttttt ctgaacattg cccatccgag tttacagtat
     1021 gtggtttcaa tgctgcaact ggaaagcaaa aaacctttga cggggacggt ttccatggtc
     1081 aacgcattgt cgtccgtcct gctggtgctt atcctgcgcg cctatctcga acaggataag
     1141 gatgtcgaac tctcgggcgt attgaaaggt tggcaggaca aacgtttggg acatttaatc
     1201 caaaaggtga tagacaaacc ggaagacgaa tggaatgtcg acaaaatggt ggcggctgcc
     1261 aatatgtcgc gcgcgcaact gatgcgccgt ttcaaaagcc gggtcggact cagcccgcac
     1321 gcctttgtga accatatccg cctgcaaaaa ggcgcgttgc tgctgaaaaa aaacccggat
     1381 tcggttttgt cggtcgcact gtcggtaggc tttcagtcgg aaacgcactt cggcaaggcg
     1441 ttcaaacggc aatatcacgt ttcgccgggt caataccgga aagaaggcgg gcaaaaataa
     1501 atcggggctt caaacgcaaa tgccgtctga aaaggctttc atacagcatt tgcgtaccgc
     1561 gtcatttcaa gggctgcatc ttcatcactt ccatcaaaaa gttggtaaat gcggggttgt
     1621 tgggtttgac atccatattt ttccaacgct gctgccagcc gcgcaaggca ttctggatat
     1681 acagcttgga ctgttccgta ttgattgcgc cccgctggct gtctatcgcc gaacgcaggt
     1741 agatttcata catactgtca tcgacggcat tgcgtccgac caggcgtttt ctgaagttgt
     1801 tcagatattg cgccgcctga accttggtca ttttaccgat acccacctga tagcccaagc
     1861 gcgtcgcttc atcgctgatt ttggcaacat ccgtccaatg cgaagaggca aggcggaaac
     1921 cttttgcagg tgcttccgtt ttgacggtat tgataggatt cacggggatt tccgtcaatg
     1981 tgggcacata aatagactgg cagccggaaa gaactgccac aatggaaaga gggataaggt
     2041 attttttcat gcccccatta taatcaagtt tgccttgaga aaacaaattg ttcggcaaga
     2101 aaaataaaat ttcggcatca gaagcaggca aaaacacatt ccacaagcct tgccgcaagg
     2161 tttacaatcc gaccgtcctt atcgcaacga ccgtttatgg ataccgcaaa aaaagacatt
     2221 ttaggatcgg gctggatgct ggtggcggcg gcctgcttta ccattatgaa cgtattgatt
     2281 aaagaggcat cggcaaaatt tgccctcggc agcggcgaat tggtcttttg gcgcatgctg
     2341 ttttcaaccg ttgcgctcgg ggctgccgcc gtattgcgtc gggacacctt ccgcacgccc
     2401 cattggaaaa accacttaaa ccgcagtatg gtcgggacgg gggcgatgct gctgctgttt
     2461 tacgcggtaa cgcatctgcc tttggccacc ggcgttaccc tgagttacac ctcgtcgatt
     2521 tttttggcgg tattttcctt cctgattttg aaagaacgga tttccgttta cacgcaggcg
     2581 gtgctgctcc ttggttttgc cggcgtggta ttgctgctta atccctcgtt ccgcagcggt
     2641 caggaaacgg cggcactcgc cgggctggcg ggcggcgcga tgtccggctg ggcgtatttg
     2701 aaagtgcgcg aactgtcttt ggcgggcgaa ccctgctggc gcgtcgtgtt ttacctttcc
     2761 gtgacaggtg tggcgatgtc gtcggtttgg gcgacgctga ccggctggca caccctgtcc
     2821 tttccatcgg cagtttatct gtcatgcatc ggcgtgtccg cgctgattgc ccaactgtcg
     2881 atgacgcgcg cctacaaagt cggcgacaaa ttcacggttg cctcgctttc ctatatgacc
     2941 gtcgtttttt ccgccctgtc tgccgcattt tttctgggcg aagagctttt ctggcaggaa
     3001 atactcggta tgtgcatcat catcctcagc ggcattttga gcagcatccg ccccactgcc
     3061 ttcaaacagc ggctgcaatc cctgttccgc caaagataaa aaatgccgtc cgaacatcct
     3121 tcagacggca tatcgggctt tatttccccg ccttcacatc ctgccactgg cgcaccataa
     3181 acttcaatgc cgccggctgg ataggcacca tgataaagct gtttttcaaa tcctcctcgg
     3241 ttgggaaaat cgtattgtcg tttttaaatt cgtcttccat cagctcacgc gccggcttgc
     3301 tcgaaggcgc ataagtaacg aaattgccgt ttttcgccga cacttccggg tcgaggaagt
     3361 cgttgatgta tttgtgcgcg ttggcgacgt ttttcgcatc tttcggaatc acgaaagaat
     3421 ccacccaaat ccccacgccc tctttgggca tcatcacgcg gattttttcc ttgccgcccg
     3481 cttcttcggc acggcgtttg gcgatgttca aatcgccgcc gaaaccgatt gttacgcagg
     3541 tatcgccgcg cgccaaatca tcgataaagc cggacgaagt aaagcgtttg atattggggc
     3601 ggtttttctt gagcagggcg gttgcctccc tgatgtcttc cgtattgctg ctgttcgggt
     3661 ttttacccaa atagttcaac accataggat agatttccgc cgcgctgtct aaataactga
     3721 taccgcattg tttgagtttg gacgtgtatt cggggtcgaa caccaaatcc cactggttgt
     3781 ccggcagctt gtccgtaccc aaagcctttt tcacgcgttc ggtattgatg gcgaaggtat
     3841 ttgtccccca ataaaacggc acggcgtatt cgtggctggg atcgaccccg tccatcagcc
     3901 tcatcatttc gggattgagg tgtttataat tgggaatcag cgatttatcg attttctgat
     3961 acgcacctgc cttaatctgc ctgcccacaa acgcattgga cggcgcgaca atgtcgtaac
     4021 cggattttcc ggtcagcacc ttgctttcca gcgtttcatc gctgtcgtac acatcataag
     4081 taaccttgat gccgtttttc ttttcaaaat cggcaacggt ttccggatcg acgtattccg
     4141 accagttgta aattttcaat acgtttcggt tttccgccgg cgccggtttt tcggcaggcg
     4201 gtttgtccga accgccgcac gctgcaagca gcaaagcagt caggacggcg agtggcagat
     4261 gtttggccat tatcattcct tgcatatcgg gttggagaaa gcggtcatta tagccgatat
     4321 tggcaacagg gcttcagacg gcattcaaaa tcccgccaca cccttccgaa aaccgccgct
     4381 tccatagcta gaaacaggga tttgcggtaa gataccgccg ttcgttttcc ctgcttttac
     4441 catgacaaga catttgagag acattgaaaa aattatgaaa acctccgaac tgcgccaaaa
     4501 attcctaaaa ttttttgaaa ccaaaggcca caccgtcgtc cgctcttcca gcctcgtgcc
     4561 gcacgacgac ccgaccctgc tgtttaccaa cgcgggcatg aaccagttta aagacgtatt
     4621 cttaggtttc gacaaacgcc cgtacagccg cgccaccacc gcgcaaaaat gcgtacgcgc
     4681 aggcggcaag cacaacgact tggaaaacgt cggctacacc gcccgccacc acaccttctt
     4741 tgaaatgatg ggcaacttct ccttcggcga ctacttcaag cgcgacgcga tccacttcgc
     4801 ttgggaattt ctgacttccc ccgaatggct caatatcccc aaagacaaac tgttggcgac
     4861 cgtttacgcg gaagacgacg aagcctacaa catctggttg aacgaaatcg gtatgccgtc
     4921 cgagcgcatc gtccgcatcg gcgacaacaa aggcgcgaaa tacgcgtccg acaacttctg
     4981 gcaaatgggc gacaccggcc cttgcggccc ctgctccgaa attttctacg accacggcga
     5041 agaaatttgg ggcggcattc ccggcagccc cgaagaagac ggcgaccgct ggatcgaaat
     5101 ttggaactgc gtattcatgc agttcaaccg cgacgaacaa ggcaatatga atccgctgcc
     5161 caagccgtcc gtcgataccg gcatgggctt ggaacgcatg gccgccgtta tgcagcatgt
     5221 tcacagcaac tacgaaatcg acttgttcca agacctgctc aaagccgttg cccgcgaaac
     5281 cggcgcagct ttcagcatgg aagaaccaag cctaaaagtc atcgccgacc acatccgctc
     5341 ctgctcattc ctgattgcag acggcgtatt gccttccaac gaaggccgcg gctacgtatt
     5401 gcgccgcatt atccgccgcg ccgtgcgcca cggttacaaa ctgggtcaaa gcaaaccgtt
     5461 cttccacaaa ctggtggccg atttggtcaa agagatgggc gatgcctacc ctgaattgaa
     5521 agaaaaacaa gttcaaatcg aagaagcgtt gaaaaacgaa gaaagccgtt ttgcccaaac
     5581 tctggaaacc ggtatggctt tgttggaaaa cgcgcttgcc aaaggcagca aaaaactcga
     5641 cggcgaaatc atcttcaaac tctacgatac ctacggtttc ccatacgact tgactgccga
     5701 catctgccgc gaacgcaata tcgaactgga cgaagcaggc ttcgagcgcg aaatggaagc
     5761 ccaacgcgca cgcgcacgcg ccgcacaaag cttcaaagcc aacgcccaac tgccttatga
     5821 cggtcaagac accgagttta aaggttatag cgaacgccaa accgaatcca aagtcctcgc
     5881 cctctacaaa gacggcgagc aagtcgacga attgaacgaa ggcgacagcg gcgcagtcgt
     5941 catcgacttt accccgttct atgcagaatc cggcggccaa gtcggcgatg tcggctatat
     6001 cttctcaggc gaaaaccgct ttgaagtacg cgatacccaa aaaatcaaag cggccgtatt
     6061 cggtcaattc ggcgtacaaa cttcaggccg tctgaaagtc ggcgacagcg ttaccgccaa
     6121 agtggacgac gaaatccgca atgccaatat gcgcaaccac agcgcaaccc acttgatgca
     6181 caaagccctg cgcgatgtat tgggcagaca cgtcgaacaa aaaggctctt tggttaccgc
     6241 cgaatccacc cgtttcgaca tttcccatcc ccaagcggta actgccgaag aaatcgccga
     6301 agtcgaacgc cgcgtcaacg aagccgtttt ggcgaacgtt gccgtcaacg ccgccattat
     6361 gagcatggaa gacgcgcaaa aaaccggcgc catgatgctc ttcggcgaaa aatatggcga
     6421 cgaagtacgc gtattgcaaa tgggcggttt ctctaccgaa ttgtgcggcg gcacgcacgt
     6481 ttcacgcacc ggcgacatcg gcctcttcaa aatcatcagc gaaggcggta ttgccgcagg
     6541 cgtgcgccgt atcgaagcca tcaccggcct gaacgcactc aaatgggcgc aagagcaaga
     6601 gcgtttggtg aaagacatca ttgctgaaac caaagcccaa accgaaaaag acgtactggc
     6661 gaaaatccaa gcaggcgcgg cacacgccaa agcattggaa aaagaattgg cacgcgccaa
     6721 agccgaactc gccgtccacg caggcgcaaa actcttggac gacgcaaaag acttgggcgc
     6781 agccaaactc gttgccgccc aaatcgaagc cgacgcagcc gccctgcgcg aaatcgtaac
     6841 caacctgacc ggaaaatccg acaacgccgt gattctttta gcggcagtaa acgacggcaa
     6901 agtctccctg tgcgccggcg tatccaaacc gttgaccggc aaagtgaaag caggcgatct
     6961 ggttaaattt gcagccgaac aagtcggcgg caaaggaggc ggccgtcccg acttggcgca
     7021 ggccggcggc acggatgccg gcaaattgcc cgaaatgctg gtaagcgtgg aaagctggct
     7081 ttgtcagaaa ttaagttaaa tttaaatctt aataagccat ttgagtcttg ttcaaatggc
     7141 ttatattttg gattgttaga atgaatacta aaacagagtt acaaaaactt ttagaagagg
     7201 atatatcaac tttaacagaa acactaattt gtgcagatgc tcttcctcca agatatgtac
     7261 gtagcattgc aactcctatt gtccgtcgat ggttaattga caaacaatta aatattctcg
     7321 caaaagaaat aggtttaact atagaattgc ccatccttga tacaagtttg gtatttgaaa
     7381 aattgtcaac cttagagaat aaagttaatt tttatatggc tggtggagtt tatttaggcg
     7441 gagaatttat tagctctata tatcattcat cacaagaatt ttctggtgaa ccaataatct
     7501 atgctgaacc aaatataatt ttatgtcctg ctgaaaaatt tttaacttta aaacgagttt
     7561 tccataatgg aaatattttt aatatgaatc agataattac ttttttatcc aataaacaag
     7621 gaggagtaca ttttgataaa aattacgata aatataaaac ttggcaagtt gctatagaaa
     7681 aagcagcaaa ttttttaaaa ctcggtaatc catataatga agataaatta tctcttagtg
     7741 aagaacatga tactatttta gtagttttac cattagaaaa aggatatgaa tggaattgtc
     7801 ttgagataga agtactatcc gctgctcaat ctctagcaaa tatctattgc aacaaagttc
     7861 gcctcataga tggtcatgtt tggaaggaat aaaagaaaaa gcatagttgg taatttttag
     7921 ttacgtaggg tgggcttcag cccaccgttt tttcattttt gattgttgat tggtgggcta
     7981 aagcccaccc tacggttact aaaatcccgt cattacctcg caggtgggaa tccggtttgt
     8041 tcggtttcgg ttttttaggg acagtgccgc aacattaaat ttccagattc ccactttcgc
     8101 acaaataaca gcgcaaatag ggcaaagata aggatatggg tttttttctt tgaatttact
     8161 atacaattcg cccgtccccg ttatagaaac ccaagccgat gaaaaccctg ctccaactcc
     8221 aaaccgccgc acaaaacttc gccgcctact acaaagacca aaccgacgaa cgccgcgaga
     8281 aagacacctt ctagaacgaa tttttcgcca ttttcggcat cgaccgcaaa aacgtcgccc
     8341 acttcgaata ccccgtcaaa gaccctgccg acaacaccca attcgtcgat atattttggg
     8401 aaggcatctt ccttgccgaa cacaaatccg ccaacaaaaa cctgaccaag gccaaagagc
     8461 aggcggaacg ttatttacag gaaatcgggc gcaccaagcc ctccgcgctg cccgaatatt
     8521 acgccgtcag cgattttgcc catttccacc tttaccgccg cgtacctgaa gaaggcgcag
     8581 aaaaccaatg gcagttccct ttggaagaat tgcctgaata catcacgcgc ggcgttttcg
     8641 acttcatgtt cggcatcgaa gccaaagtcc gccaaattca agaagaagcc aacattcaag
     8701 cggcggcgac catcggcagg ctgcacgacg cgctcaaaga agaaggcatt tacgaagaac
     8761 acgagctgcg cctcttcatc acgcgcctgc ttttcctctt ttttgccgac gacagcgccg
     8821 ttttccggcg caactacctt ttccaagact ttttagaaaa ctgcaaagaa gccgacacgc
     8881 tcggcgacaa gctcaatcaa ctctttgaat ttctcaacac acccgaccaa aagcgcagca
     8941 agacccaaag cgaaaaattt aaaggtttcg aatacgtcaa cggcggtctt ttcaaagaac
     9001 gcctgcgcac tttcgacttc actgccaagc agcaccgcgc cttaatcgac tgcggcaatt
     9061 tcgactggcg caacatcagt ccagaaatct tcggcacgct cttccaatcc gtcatggacg
     9121 cgcaagagcg gcgcgaagcg ggcgcgcact acaccgaagc cgccaatatc gacaaagtca
     9181 tcaacggcct ttttttagaa aacctgcgtg ccgaatttga agccgtcaaa gccctcaaac
     9241 gcgacaaagc caaaaaactc gccgccttct accaaaaaat ccaaaacctg caattcctcg
     9301 accctgcctg cggctgcggc aacttcctta tcgtcgccta cgaccgcatc cgcgcccttg
     9361 aagacgacat catcgccgaa gccctcaaag acaaagcaga cggcctgttc gacagcccgt
     9421 ccgtccaatg ccgtctgaaa cagtttcacg gcatcgaaat agacgaattt gccgtcctca
     9481 tcgcccgcac cgccatgtgg ctcaaaaacc accaatgcaa catccgcaca caaatccgct
     9541 tcgacggcga agtcgcctgc catacgctgc cgctcgaaga cgccgccgaa atcatccacg
     9601 ccaacagcct ccgcacacct tggcaggcgg cggactacat cttcggcaat ccccccttta
     9661 tcggctcgac ctaccaaacc aaagagcaga aaaacgacct cgaaagcatc tgcggccata
     9721 tcaaaggcta cggcctgttg gattacgtct gcaactggta cgtcaaagcc gcaggcatca
     9781 tggcgcagca tccccaagtt cagacggcat ttgtttccac caattccatc tgccaaggcc
     9841 agcaggtcga aatcctctgg ggcagccttt taaaccaagg catcgaaatc cactttgccc
     9901 accgcacctt ccaatggacg agccaagccg caggcaaagc cgccgtccac tgcatcatcg
     9961 tcggcttccg ccaaaagccg ccaatgccgt ctgaaaaaac cctctacgac tatcccgaca
    10021 tcaaaggcga acccgaaaaa cacgccgtag ccaacatcaa tccttatctg atcgatgcgc
    10081 ccgatttgat tatcgccaag cgcagccgtc ccatacattg cgaacctgat atggtcaacg
    10141 gaagcaaacc gaccgaaggc ggcaacctta tcctttcaac cgccgaaaaa gatgccctga
    10201 ttgccgccga acccttggcg gagcaataca tccgcccctt tatcggcgcg gatgagtttc
    10261 tcaacggcaa aacccgttgg tgcctgtggt ttcacggcgt atccgatgtc aaacgcaacc
    10321 acgacctgaa acaaatgccc caagttcaag cccgtattca ggcggtcaaa accatgcgcg
    10381 aagccagcag cgacaaacaa actcaaaaag atgcagcaac cccgtggctt tttcaaaaaa
    10441 tccgccagcc ttcagacggc aattatctga ttattccgag cgtgtcgtct gaaagccgcc
    10501 gtttcatccc catcggttat ctgtcgtttg aaacagttgt cagcaatctg gcatttatcc
    10561 ttccaaacgc caccctctac cacttcggca tcctcagctc caccatgcac aacgccttta
    10621 tgcgtaccgt tgcaggtcgt ctgaaaagcg attatcgcta ctctaatacc gtcgtgtaca
    10681 acaacttccc cttccccgaa agctgccggt tgccgtctga aaacgaccgc cccgacccgc
    10741 tccgcgccgc cgtcgaagcc gccgcccaaa ccgtcctcga cgcgcgcgga caataccgcc
    10801 gagaagcgca ggaagccggt ttgcccgagc cgaccctcgc cgaactctat gcgcccgacg
    10861 caggctatac cgccctcgac aaagcccacg ccaccctcga caaggcagtc gataaagcct
    10921 acggctacaa aacaggcaaa aataccgacg acgaggcaga acgcgtcgcc ttcctgttcg
    10981 agctgtaccg caaggcggcg gcaattgcgt agtagttgcg tagtagttgc gtagtagttg
    11041 cgtagtagtt gcgtaggata gttgcgtagg gtgggcttca gcccaccaac tcccaccaac
    11101 tcccaccatc ttcaccctaa aaccccgtca tctccgcaaa agcaattgcg tagggtgggc
    11161 ttcagcccac cgtttttctt cattttcggt tgttgattgg caggctgaag cccaccctgc
    11221 ggttacaaaa atcccgtcat tcccgcgcag gcgggaatcc agacctttcg gggcggaaac
    11281 ttatcggata aaacgatttc ccctgcatcc tggattccca ctttcatggg aatgacggga
    11341 tataggtttc cctacggacg tgttcagatt cccgccttcg cgggaatgac ggcggagaag
    11401 tttctgtctt tccgataact taccgtaact taaaatcccg tcattaccgc aaaagcagtt
    11461 gctacggtta caaaaatccc gtcatcccca cgaaagcgaa aatcctgcct gtcggatttc
    11521 ggttttttcg ggcgtttcgg gaacttataa atcgtcattc ccgcgcaggc gggaatccag
    11581 aacgcgggac ggcggcaata ttcaaaggt
//
LOCUS       DAWKOD010000051        11186 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000051 DAWKOD010000000
VERSION     DAWKOD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.051"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1184)
                     /locus_tag="WJK73_001023"
     CDS             complement(<1..1184)
                     /locus_tag="WJK73_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7557516.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WR"
     gene            complement(1189..1842)
                     /locus_tag="WJK73_001024"
     CDS             complement(1189..1842)
                     /locus_tag="WJK73_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ7557517.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            1993..2283
                     /gene="gatC"
                     /locus_tag="WJK73_001025"
     CDS             1993..2283
                     /gene="gatC"
                     /locus_tag="WJK73_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ7557518.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            2346..3791
                     /gene="gatA"
                     /locus_tag="WJK73_001026"
     CDS             2346..3791
                     /gene="gatA"
                     /locus_tag="WJK73_001026"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ7557519.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRTAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPIGV
                     QLIGNYFSEAKILGAAHQIQLNSDWHGKRPE"
     gene            3788..4720
                     /locus_tag="WJK73_001027"
     CDS             3788..4720
                     /locus_tag="WJK73_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ7557520.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            4763..6193
                     /gene="gatB"
                     /locus_tag="WJK73_001028"
     CDS             4763..6193
                     /gene="gatB"
                     /locus_tag="WJK73_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ7557521.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            6285..7295
                     /locus_tag="WJK73_001029"
     CDS             6285..7295
                     /locus_tag="WJK73_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ7557522.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(7425..8057)
                     /gene="pdxH"
                     /locus_tag="WJK73_001030"
     CDS             complement(7425..8057)
                     /gene="pdxH"
                     /locus_tag="WJK73_001030"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ7557523.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            8278..9327
                     /locus_tag="WJK73_001031"
     CDS             8278..9327
                     /locus_tag="WJK73_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7557524.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(9396..10937)
                     /locus_tag="WJK73_001032"
     CDS             complement(9396..10937)
                     /locus_tag="WJK73_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ7557525.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         2843 a         3393 c         2788 g         2162 t
ORIGIN      
        1 aaccgccaag cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa
       61 tcattggcaa gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      121 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      181 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
      241 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
      301 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga
      361 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg
      421 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa
      481 tcggcatcgg gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg
      541 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc
      601 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
      661 aaaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
      721 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
      781 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
      841 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
      901 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
      961 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1021 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1081 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg
     1141 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1201 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     1261 catacccata atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     1321 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat
     1381 caacgcgatg tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc
     1441 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     1501 ggcggggcgc gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     1561 gttgccgttc atcatagaaa tggcaatcag caacaccaaa attccgccgc cgacctgaaa
     1621 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     1681 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     1741 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattga tcagcaccaa
     1801 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     1861 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     1921 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     1981 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2041 ctgcacctga ctgcggcaga aaaagaaaaa tcgcttcaag aattaaacga cattttcgct
     2101 atggtcgaac agatgcagaa catcaacaca gacggcatcg aaccgatggc gcatccgcac
     2161 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     2221 caggcggtcg caccggaagt acgcgaccgc ctgtacatcg taccgcaagt tatcgaagaa
     2281 taatccgaat acgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     2341 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     2401 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     2461 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     2521 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     2581 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     2641 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     2701 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     2761 aaaacccatg gaatcttgag cacgtacccg gcggttcgtc aggcggttcc gccgccgtcg
     2821 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac
     2881 ccgcatcgca ctgcggtatc accggtatca aacccacata cggcacggtt tcccgcttcg
     2941 gtatggtcgc ctacgcctcc agcttcgatc aagccggccc gatggcgcaa actgccgaag
     3001 actgcgcgat tctgttaaac gcgatggcag gtttcgaccc gaaagactcc accagcctcg
     3061 agcgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg
     3121 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttctgact gcattgcaaa
     3181 acaccattga tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa
     3241 ccaagctgtc cattccggcc tactatgtcc tcgcctccgc agaagccagt accaaccttt
     3301 cacgttacga cggcgtacgt tacggacacc gtaccgccca attcggcgat ttggaagaaa
     3361 tgtacggcaa aacccgcgcc gaaggttttg gcagcgaagt caaacgccgc atcatgatcg
     3421 gcacttatgt actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     3481 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     3541 cgaccgcacc ctccgccgca cccaaaatcg gagcggatgc ttcgccggtt gaaacctact
     3601 tgagcgacat ttacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     3661 caggtttcag cggcggcgga ctgcccatcg gtgtacagct tatcggcaac tatttctccg
     3721 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     3781 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     3841 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     3901 agaaatttac gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct
     3961 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4021 caaacgccgt ttagatgcga tgacggcaga ttttcttatg gcggaaaaac tgttcggcac
     4081 agacattctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4141 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4201 atctttgcgc gacgaagcag gcaaccggct gtatatggcg accttcgcct ttgtcggcac
     4261 acacctgctg acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg
     4321 ccgcataacc aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     4381 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     4441 caaactgcgc tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga
     4501 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     4561 agaccttacc gacatcgaaa gcaaaaaacg ctcgatgtac cgcaaacgtt atgaaatgct
     4621 ggacaacatg gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga
     4681 cggcatccaa acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga
     4741 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     4801 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     4861 caacgcgcac gccggcgtgg tggaatgtgc tttgccgggc gtactgccgg taatgaaccg
     4921 agaagtcgtt gaaaaagcca tcaaattggg tttggctttg gatgcgaaaa tcaaccggaa
     4981 aaacgtgttc gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag
     5041 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5101 gaaaaccatc aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga
     5161 aggcttgaac ggcgcgaccg gtatcgattt gaaccgcgcc ggcacgcctt tattggaagt
     5221 cgtatccgaa cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca
     5281 cagcttggta acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt
     5341 cgatgccaac gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat
     5401 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca
     5461 aatcgagatt ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga
     5521 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagacgcg cacgactacc gctacttccc
     5581 tgaccccgat ttgctgcccg tcatcatttc agacgaccgg ttgcaaaaag caaaagcaga
     5641 aatgcccgag ctgccaaaag aaatggcggc gcgtttcgtg gcggattatg gcgtgagcga
     5701 ctacgacgcg cgcctgctca ctgccagccg cgtgcaggct gcctattttg aagaagccgc
     5761 taaagagagc ggacaaggca agctgactgc caactggatg aacggcgaac ttgccgccac
     5821 gctgaacaaa gaaggcatgg aacttgccga cagcccgatt accgccccac gcctcgccgc
     5881 tctggtaggc aaaatcgccg acggcacatt aagcagcaaa ttggcgaaaa aagcctttga
     5941 agccatgtgg gctgaacccg aagcctccat tgccgaaatc attgaaaaac acggtttgca
     6001 acagatgacc gataccggcg cgattgaagc gatggtagac gaagtgctgg caaacaatgc
     6061 aaaagccgtg gaacagttca aatccggcaa cgaaaaagcc ctgaatgcga ttgtggggca
     6121 agtgatgaag gccagcaaag gcaaagccaa tcccgcgcag gttcaagagc tgattaaagc
     6181 caaacttgcc tgataagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt
     6241 ttcacagtct gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccatta
     6301 cactgcccga ccaaaccacc tttgccgccg gcgacggtga aaccgtttta gccgccgccg
     6361 cccgtcaaaa cctcaatctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag
     6421 ccgaactggt cagcggcgat attcaaatgg gcggacactc ggaacaggct ttatccgaag
     6481 cagaaaaagc gcaaggcaag attttgatgt gctgcaccac tgcgcaaagc gatatcagca
     6541 tcaacatccc cggctacaaa gccgatgccc tacccgtccg cactctgccc gcacgcatcg
     6601 aaaatattat tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc
     6661 cgtttgcctt ctacgctggg caatacattg atttactgct gccaggcaac gtcagccgca
     6721 gctactccat cgccaatccg cccgaccaag aaggcatttt ggaactgcac atccgcaggc
     6781 gtgaaaacgg tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag
     6841 gcattgtccg cgttaaaggc ccgctcggtt catttaccct gcaggaagac agcggcaagc
     6901 ccgtcatcct gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc
     6961 ttatccgcca aggcagcagc cgcgtcgtcc atttctactg gggcgcgcgt catcaagatg
     7021 atttgtatgc cctcgaagaa gcacaagggt tggcagaccg tctgaaaaac gcctgcttca
     7081 cccccgtatt gtcccgcccc agagagtgct ggcaaggaag aaaaggccac gtacaagaca
     7141 tcgcggcaca agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg
     7201 ctatgaccga acaggcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt
     7261 ttttctccga cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg
     7321 ctttccgttc ggaacacaaa aaacttcccg tccgtatttt ccccgtgaaa aaatgccgtc
     7381 tgaaacccga ttcctgtttt cagacggcat atgttttttc ctgttcaagg cgacagccgc
     7441 tcgcgtatcc agccgccatc cagcaaacgg tattggatgc ggtcgtgcag cctgctcggc
     7501 ctgccctgcc aaaactcaat caaatcggga atcacaatat agccgcccca atgcggcgga
     7561 cgcggcacgt gcaaaggatg tttgagtccg accgccgccg cctttgccac caataccgcc
     7621 ttgttcggaa tcacttcgct ctgcgcgctt gcccacgcac ccaaacggct ctgatacggg
     7681 cggctctcaa aatattcgtc cgacaatttc tccgccagcc tttcaacacg cccttccacg
     7741 cgcacctgac gctccagctc cggccaaaaa aacgtcatcg ccgcaaatgg atgagcatcc
     7801 agcgaacgcc ccttgcggct gtgataattc gtaaagaaaa caaaaccttc agaattaact
     7861 tccttcagca gcaccatacg gctgttgggt ctgccgcgtc cgtcgaccgc cgccacatta
     7921 accgccgtcg gctcgttaac ctgtgcgcgt accgcctcgt ccaaccaccg ctcgaactgc
     7981 tcgatcggat tgtcggcgca atcgccttcc gacaattccc gtttgctgta atcttcccga
     8041 atattgtgca aatccattta ccgctcctct tgttatttga aagattctac ccccgcacgc
     8101 aaaccgattt caaccgttgc acaaactttg tccccaaagc cgcagcgacg atttcatccg
     8161 caaaaccgcc gcatcaggta caatatcgaa ccgtccgact gaggacggca ttttatcaac
     8221 ccgtcctgcc gcatacgccg cagaagaacc gctttatcaa gcgagttagg aaaaatgatg
     8281 tccaaacagc ccaccagcaa acgccaatgg cgcgacggcg cagccccgtc tgccaagaaa
     8341 accgccaaac cgttcaaaag caaagcccgt cccaaagacg aaacggacaa aaccgcatcc
     8401 caaccttacg gacaaaaagc ttcagacggc atcaaacctc aaaacctccc caaacagcgc
     8461 gccgccaaag ccaaaaaact cgtcgtccgc aatcccaacc aaaaaattat ggaacacgcg
     8521 cgcgatttaa aagaacgccg cagcgaccta tcgcgcatgg aacccgaacg cctgcaaaaa
     8581 gtgcttgccg cgtccggcgt cggctcgcgc cgcgaaatgg aagaatggat caacaacggc
     8641 tgggtaacgg tcaacggcaa aaccgctcaa ctgggcgaca aagtcacccc cgacgaccac
     8701 gttaccgtca aaggcagcat catcaaactc aaatgggccg accgcctgcc gcgcatcatc
     8761 ctatattaca aacaagaagg cgaaatcgtt tcccgcgacg acccgcaagg ccgcgtcagc
     8821 atattcgacc gcctgccgca ggccgccagc agccgctggg tcgccatcgg acgcttggac
     8881 atcaacacca gcggacttct gattctcacc acctccggcg aactcgtcca acgtttcgcc
     8941 caccccagct tcgaagtcga acgcgaatac gccgtgcgcg tactgggcgg gctgaccggc
     9001 gaacaaatgc gcgtcctcac cgaagaaggc gtgatgctcg aagacggctt ggcaaaagtc
     9061 gaacgcatcc gcgaacaagg cggcgaaggc gcgaacaaat ggtacaacgt cgtgattaaa
     9121 gaaggccgca accgcgaagt gcgccgcatt tttgaaagcc aaggactcac cgtcagccgc
     9181 ctcgtgcgcg tcggcttcgg ccccatcgga ctgcccaacc gcctcaaacg cgggcagttc
     9241 tacgaactca accccgccga agtcgccaac atcctcaaat gggcggaaat gctgctgccg
     9301 ggcgaatacc gccgcaaaaa agcctaaacc cgccaaaaca caaaaatgcc gtctgaaaca
     9361 tctgctgttt cagacggcat tttgttcggg cgttttcagg agaaaaggtc gagtgctttg
     9421 acaaagatca tcaccacgcc gtaggcgagc ggtatgacgg ccaatgccca acgccaccag
     9481 accgatatgc cgccgccgga aactttttcg cccacaaggt aagcgtcgga tatggcggtt
     9541 tcgtcatcgg ggttgccgct gtgtgcggcg gttttgatgt ctttttcgtg gtgtttttcg
     9601 tgtacggatt tgacggcgag gttgcacaac aaaccgataa tcagcagacc cgccatgatg
     9661 tacatggtta cgctgtatgc ctgtgccgcc ggtatgccgc tgtcgatttg gctttggcgt
     9721 atgtaattga ccagtaccgg gccgatgacg gcggcggttg accaggccag caggatgcgt
     9781 ccgtgaatcg cgccgacctg ataggtgccg aacaggtctt tcaggtaggc gggaatggcg
     9841 gcaaatccgc cgccgtacat ggaaataatc acgcaaaagc cgatgatgaa cagggctttg
     9901 ctgccgccct cgccgatgga gggaacggcg aaatacagca gcgaaccgag tacgaagaag
     9961 atggtgtagg tgtttttgcg tccgattttg tcggaaacgc tcgaccacaa aaagcgtccg
    10021 cccatgttaa acaggctcag gaggctgacg aagcctgccg ccgcacctgc gccgactgcc
    10081 gcctgcctgc ctatggaggt ttcggaaaag agttcctgaa tcatcacgga tgcctgaccc
    10141 aatacgccga tgccggcagt tacgttcagg cacaataccc agaacaacag ccaaaactgc
    10201 ggcgttttca tggcttggga cacgttgaca tgattgctgc tgaccagctt gttttgcgtt
    10261 ttcggcgcgg tatagccttc aggtttccag ccgtcggcag gtacgcggat ggtaaacgcg
    10321 ccgaacatca tcagtgcgag gtaaagcaga cccaatacgg cgaaggtttc ggcaacccca
    10381 accgaagcag cgtttgaaaa ggtgttcatc agtgatacgg aaagcggcga ggccagcatt
    10441 gcgccgccac cgaaacccat aatcgccaaa ccggtcgcca tacccggctt gtcgggaaac
    10501 catttcatca gtgtggaaac cggcccgatg tagcccaaac ccaagcctac gccgccgatg
    10561 acgccgttgc ccaaatagag caggaagagg ttgtgcgtac gcacgccgaa tgcggatacg
    10621 aagaagccca ggctgaaaca gcaggcggcg gcaaatatgg ctttgcgcgg ccctacccgt
    10681 tccatccacg taccgaacag ggcggccgac gcgcccagca tcgcgagtgc gatactgaaa
    10741 atccaaccta cggtcgtcag cttccaatct ccggccgccg attcggttat gccgataagt
    10801 ttggtcagcg gcgcgttgaa tacggaatag gcgtaaatct gcccgatggc aaggtgtacc
    10861 gccaatgctg cgggcggtac gagccaacgg ttgaaacccg gcttggcaat gcttgcctca
    10921 cggtctaaaa acttcataac atcctctttc tgtcagttga aaaataaaat ttcatttgct
    10981 caatggaaac ttattgaaaa ttataaaaaa atatcgggtc gggtttttat ccgccccaag
    11041 atgcgccgtc tgaaacattt cgggtgtacg gaaaggtttc tgttttttcc gacaaattcc
    11101 tgcggctttt cgcttccgga ttcccgcttt tgcgggaatg acgaattaaa gattatctta
    11161 aggtcaaggg actggattcc cgcttt
//
LOCUS       DAWKOD010000052        10949 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000052 DAWKOD010000000
VERSION     DAWKOD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.052"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..546)
                     /locus_tag="WJK73_001033"
     CDS             complement(<1..546)
                     /locus_tag="WJK73_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7557526.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(578..961)
                     /locus_tag="WJK73_001034"
     CDS             complement(578..961)
                     /locus_tag="WJK73_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7557527.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1330..2034
                     /locus_tag="WJK73_001035"
     CDS             1330..2034
                     /locus_tag="WJK73_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ7557528.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2098..2664)
                     /locus_tag="WJK73_001036"
     CDS             complement(2098..2664)
                     /locus_tag="WJK73_001036"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ7557529.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2730..3146)
                     /locus_tag="WJK73_001037"
     CDS             complement(2730..3146)
                     /locus_tag="WJK73_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557530.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3198..4097)
                     /locus_tag="WJK73_001038"
     CDS             complement(3198..4097)
                     /locus_tag="WJK73_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ7557531.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJK73_001039"
     CDS             complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJK73_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ7557532.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5739..6368)
                     /locus_tag="WJK73_001040"
     CDS             complement(5739..6368)
                     /locus_tag="WJK73_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ7557533.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJK73_001041"
     CDS             complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJK73_001041"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ7557534.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNFLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7758..8300)
                     /locus_tag="WJK73_001042"
     CDS             complement(7758..8300)
                     /locus_tag="WJK73_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7557535.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8696..9232)
                     /locus_tag="WJK73_001043"
     CDS             complement(8696..9232)
                     /locus_tag="WJK73_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557536.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9400..9600)
                     /locus_tag="WJK73_001044"
     CDS             complement(9400..9600)
                     /locus_tag="WJK73_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557537.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(9715..10134)
                     /locus_tag="WJK73_001045"
     CDS             complement(9715..10134)
                     /locus_tag="WJK73_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557538.1"
                     /translation="MSFGYLIATSQPYELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(10192..10557)
                     /locus_tag="WJK73_001046"
     CDS             complement(10192..10557)
                     /locus_tag="WJK73_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557539.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(10539..>10949)
                     /locus_tag="WJK73_001047"
     CDS             complement(10539..>10949)
                     /locus_tag="WJK73_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557540.1"
                     /translation="LSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVR
                     DVAIAGGLGAIPGGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHR
                     RVTDNTGKTLPGQGIGRHRPWESKSTDRSWKNRF"
BASE COUNT         2681 a         2623 c         2641 g         3004 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgtcgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgttcag gcgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagtagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttgggggagg tactgccatt cgcagccgta tttgtgttgc aggacgtgtg
      661 cgaaatggcg gctgacagcg agcagtgcgg aggcgtgcgc cgctgctttt tccatcggct
      721 gccattggtg gggacggatc agaccgcgcg tgatggtgct gctgtgttcc gtgacgacat
      781 aggggatgcc gtatttttgg gaaatttttt gggcgagtat gccggcatag ttcatgcggt
      841 gggcgtgaat caggtcgggc agcccgtttt cgcggatgta gtgtttgaaa gctttcaaac
      901 ccgcacacac ccagcggatg cagtcgatgt cgatgaacgg aaagcggggg aagaaatata
      961 tgccgtgcca tgcatagatg tccaaaccgc tttgccgata ttttgcaaaa ccgtaaggcc
     1021 ggtcaggatg cttgctgttt ctttccgcaa gtagcggaac atcggtgcaa gcacggcggt
     1081 tttgatgcct ttcctctgca atgccagtgc ctgattttga aaaaaatccc gtccacatcc
     1141 tgttcggatt gcggatacca tgaggggatg acgaggacgt gcaagggttc gggcatagtg
     1201 ggattccgta tcggaaaggc ggttattata agacagacgc agaccgaata tttaaattgt
     1261 tgccttacgc taatgcaatt tagcgcgccg atatgctaga ttggcggttt tataagtaag
     1321 gatacggata tgttgcgttc tattttggcg gcttccctgc tggcggtatc ttttccgacg
     1381 gcggctgaag cattgaatta caatattgtc gaattttctg aatcggcggg tgtcgaggcg
     1441 gttcaggata caatgtccgc acgtttccaa gtgacggcgg aaggacggga caaaaatgcc
     1501 gtcaatgccg agtttgttaa aaaattcaac aatttcacca gaaaatcaaa aaatggtagc
     1561 tttaaaaccg aattggtatc gcgcagtgcg atgccgcgct atcaatatac caacggcaga
     1621 cgcattcaaa caggttggga ggagcgtgcg gaatttaagg tcgagggtag ggattttgat
     1681 gcgttgaacc gttttattgc cgatgttcag gcagatgccg cgttggaata tacggatttc
     1741 catgtgtcgc gcgaacgccg caacgaggtc atcgatcagg tcagcaagga tgccgttttg
     1801 cgtttcaagg cgcgtgccga aaagttggcg ggcgttttgg gtgcgtccgg ttataaaatc
     1861 gtcaaattga atttgggaca catcggcagc catatcgcgg gagggggagc tgctcaggca
     1921 aaaatgcttc gtgccatgcc gatggcggca agcgtcaata tggagggtgc ggattccgcc
     1981 gcgcctggtg tggaggaaat cagcatcagc gtcaatggga cggttcagtt ctgatttgag
     2041 gtgaacggca aatgccgtct gaaacccgac gataagggtt cagacggcat ttatatttca
     2101 ggctttgggc agggtaacgc cggtttgccc catatatttg ccgttgcggt ctttgtatga
     2161 ggtttcgcag atttcgtcgc tctcgaagaa gaggacttgc gccacgcctt cgcctgcgta
     2221 gattttggcg ggcagggggg tggtgttgga aaattcgagg gtaacgtagc cttcccattc
     2281 cggttcgaac ggggtaacgt tgacgataat gccgcagcgg gcgtaggtgg atttgcccaa
     2341 gcagacggtc aggacgttgc gcgggatgcg gaaatattcg accgtgcgcg ccagtgcgaa
     2401 ggaattgggc gggatgatgc agcagtcgtc ttcaacggta acgaagtttt tcgggtcgaa
     2461 gtttttggga tcgacgatgg tgctgttgat gttggtgaag attttaaatt cgtttgcgca
     2521 gcggatgtcg tagccgtagc tggacgtacc gtaggagatg atgcgtttgc cgtcggcttc
     2581 tttgatttgg ttcggctcga aagggtcgat catgccgaat tcttcgctca ttcggcgtat
     2641 ccatttgtcg gacttgatgc tcataatgtt ttccttgttt cttgcagtgt tcggacaaag
     2701 cattggggga tgccgtctga aaacggggct tatttgtttt tgggcagctt cacttcttta
     2761 atcatgccgt tttcgcattt cataatgaag agggtttcgc cgttttgcga gacggtaaat
     2821 ctgccgtgtg ccaagccttt tttgaatgtg cccgagagta ccatgttgcg gaatttggta
     2881 ctgtcggaat tgaacggttc gataaatatt tcgcggttgg cggcaacggt ataaacgcct
     2941 tgtccgtcga atttgccatt tttaaacgaa ccggtatagt tgcgcccgtc ttggcagcgc
     3001 catgtgcctt tgccggcggg tttaccgtct ttgccgacat cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggac gcggcgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ctgacggtat
     3181 aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag ctcttccaac
     3241 atgatgctga cggattccgc catcaggatt tgcgaggcga aggcgagacc ggcggcatcg
     3301 tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg cttgagtgtg
     3361 aagtcttgcg tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag ttgggtaacg
     3421 gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg tttggatact
     3481 ttgacgacgg gaacaatatc gcccgtgcct ttgagttcgc aatcgctgtc caattcaaaa
     3541 ccgccttcgc aatgcccttg caacagccag ccggtcatca gggaagaggg cgattgtttg
     3601 gtattcggca gcgaggcttc caaaccgccc aaagcttcgc gcagcttggt caggatgttt
     3661 tccgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt gttaaacacc
     3721 gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt aatttgctct
     3781 ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg gatttccgct
     3841 accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc ctcttttttc
     3901 agggcgacgc gcaaggtaaa gtcggcaggg aaaacgagtt ccggggagaa tgaaaccggt
     3961 gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc agcttcagca
     4021 agtttgtcgg caagtacgtc cgcctcaggc agcttttctt tgttgagcgg ataaaaacta
     4081 atctgcttga accacataat gtttcctgtt gtttgaaatg tcgggaatta tttgctgaat
     4141 tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc tttgcggcct
     4201 tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt cttcggtttg
     4261 tcgtcggcgt tttcatacgg gttttccgaa acattgtatt gaatccgcaa cggcgtgcct
     4321 tgcaggttga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct gtcggaaatc
     4381 gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc ttggtgggca
     4441 taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc gattgcgctt
     4501 tgcaggacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt gtaggcagcc
     4561 tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat aaagtggaac
     4621 ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg ctcgcgacgt
     4681 tcttcgctga tgccgtccca tttattgacc gccaccacca aagcacggcc tgcctccaaa
     4741 gcgaaacctg caatcgtcgc atcttggtcg gcgatgtcct gctgcgcgtc caataccaaa
     4801 acagcgacgt ttgccgcttc aaccgcctgc atcgctttga taacggagaa tttttccact
     4861 gcttcatcca ctttgccgcg acggcgcaca cctgcggtat cgatgatggt aaacggtttg
     4921 ccttcgcgct cgaaatcgat atggatactg tcgcgcgtcg tacctgccat atcgaaggcg
     4981 atgacgcgct cttcgccgag aatggcgttt accaaggttg atttaccgac atttggacga
     5041 ccgataacgg caaaaacagg atgtttcgcc tcttcctctt ctttttcagg ctcagggaag
     5101 gtttccaaaa tatcttcaat cagataatac acgccgtcac cgtgcgcacc tgaaataaca
     5161 taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct gttgccgcct
     5221 tctcctttat tcacggccaa ataaacgggg cgcgggcttt ggcgcaaacg gtcggcaatg
     5281 attttatctt gcggcgtcag accggtacgg ccgtccacca aaaacacaac cgcatcggct
     5341 tcatcgacag cctgtaaggt ttgttttgcc atttcgtgca aaatgccgct gtccacaacc
     5401 ggctcgaaac cgccggtatc gatgaccaaa taaggtttgc tgccgacttt gccgtgtccg
     5461 taatggcggt cgcgggtcag gccgggcagg tcgtgtacga gtgcgtcttt ggtgcgcgtc
     5521 aaacggttga ataaagtaga tttgccgacg ttggggcggc cgacaagcgc gatggttggt
     5581 ttcatgattg agtctttctg tgtcaagtgc cgttcgggag aactgaacac gagcaggtgt
     5641 ccgttggaca cggtaaatag gttcttcacg gcaagaggcc ataaagaggg agggtaaaat
     5701 gccgtctgat aaataatgga cggcatttca gacggcattt atttcagcga atcaagtttc
     5761 atttgaacca attcgcgacc gacagaatct tgaggcattt tttctaaagc ctgtccgtag
     5821 ttttttaagg cttcctggct ttttccctgt gcggcataga catcgccttt ggtttccatc
     5881 agcagggggg cgaagtccgc ttcaaccggc gtgtcgagtg cggcaagcgc ggcatcgtat
     5941 tttttttgtt gcaacaacac aacgcccaga cgctgcgccg ccaacgcctg gatcaggctg
     6001 tctttttggt tggacaatac ccatttcaaa tggccttcgg caacatcgta acgctgcgcg
     6061 tcaaattcgg ttgctgccgc catcagcgtg gcttgggcgg cggaaatgga atgggggtag
     6121 ctttgttgga gcttggccaa ttcggcattg atttcgcttt gcggggcttt gttttgcgcc
     6181 ttttccacga tgtttgccag caccgccgcc gcttcctgat tttgggaagc cgcacggttt
     6241 tggtaaaccg tgtatcccaa gtagccgagt gccgccaaaa tcagcacggc aaacagccat
     6301 ttgcccgtgg ttttccaaaa atatttaaag ttgtccaact cttgttgttc ttccaaatgg
     6361 gctgccattt atgcgttctt ccattgttgt aaagtgtttg tcaaatcagc ggcggcgacg
     6421 gtttgctgac cgtgtgcgcc gttcatatct ttgagcgtaa ccgtaccgtt cgccagttcg
     6481 tcttgcgcga caatcagggc aaagcgtgcg ccgctgttgt cggctttttt catttgcgct
     6541 ttcaggctct gatagccgga atgctgcatc acattgaaac cttgcgcgcg caaggcttgt
     6601 gcgtatttca tcacctgcaa gtccgcccct tcgccttggt gcattgcata gacatcaggc
     6661 gcggcgttta cttccagcga gccgtattcg ctcaccaaaa gcagcagccg ctcgatgccc
     6721 attgcaaagc cgatagacgg cgcaggctta ccaccaagtt cctcaatcaa accgtcgtaa
     6781 cggccgccgc cgcacacagt tgcctgcgcg ccgagtttgt cggtcgtcca ctcaaaaacc
     6841 gtttgattgt aataatccaa accgcgaacc aagcgcggat tttcaatata ttggataccc
     6901 aaaccgtcca acatcgcttt gaagcgtgca tagtggtttt gcgaagcctc gcccaaataa
     6961 tccaccagac gcggtgccgc attgcagatt tcctgcaaat cagggttttt cgtatccaaa
     7021 acgcgcaaag gattggtttt cagacggcgt ttgctgtctt catccaattt atcttcataa
     7081 cgggtcagat attcaaccaa tgccgcacgg tgtgccgcgc gttcctcacg gttgcccaag
     7141 ctgttgattt ccaaagtcag gtattcgcgg atacccaatt tttcccacaa gtcggcagac
     7201 atcgcgataa tttccgcatc gatatccggc ccttcaaaac ccaaagcctc gataccgacc
     7261 tgatggaact gacgataacg gcctttttgc ggacgctcgc ggcggaacat cggccccata
     7321 taccacagct tttgcgggct gttgtacaga aagttgtgtt cgaccaccgc acgcaggcag
     7381 gaagccgtac cttcaggacg caagctcaaa ctcaaagaat cgtttgaatc ggagaaggtg
     7441 tacatttcct tgccgaccac atcggtttcc tcgccgatgg agcggacaaa caaaccggtt
     7501 tgctcgacaa tcggcgtacg gatttgctga taaccgtaag tgcgtgtcca gcggccgacc
     7561 gtatcttcaa acgcctgcca aaacgcagcc gtcagtttga aatctttttg ctcgacaggc
     7621 agaaggtcgt tcatgccttt gacggattgg attttttgtg ccatttcaag taagaatgct
     7681 taaatcaaat tgcgggcgat tatagcggat ttttaagggt ttgtgaggtt gggggtggtt
     7741 tgcggacggc atttgactta ctctgcacgt gcttgcctga tttgtccgac tgcaaactcc
     7801 gtctgccgct tgggatgatg tgtgaaaaac aatttatttg aaattgtctc ggcttttttc
     7861 ggcacgaccg ccaaaatctt acctgccaaa tttccctcac gggtttgcca agcatccaaa
     7921 aactgcgccc tgctcattga aacatgaccc agcgacgggt cggcaagcaa aaccgtattg
     7981 ccgtctatcc cgcgcaatac cgagaaatga tcatccttgc ggtatttcag atacacgatg
     8041 acggggattt tcaactgtgc gagctgctcg aaagacaggg catagccttt cgcttcaaaa
     8101 cccaaatctg gcataatgcg ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc
     8161 agctttttca acacttcttc ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc
     8221 gccaccgaag ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc
     8281 ctttctttcc aactctgcac tttgattttt ccgtaaacaa caggattatc gttaaacatc
     8341 ggtgcagcat tcaaacgata agataaagaa acgacaacac acgccaacag aaaaacatat
     8401 ttgaacttca tcatattgtc cacataaagg gcagcctgaa aatctttcag gctgcccttg
     8461 tcaaattatt cctagctttc ggcttttttg gcaaaccaaa caatccgatt acccgcataa
     8521 tactttccat ttattgaaat ccgacaagcc gcgcccaaaa aatgccatgc actgtcgatt
     8581 tccgcagtaa tctttgtatc gttttcttca aattccaaat attcacccaa taataaactt
     8641 gaaacagaac gcgtgggcag caagtgccca ccctacgcct attcaaataa tttgattaaa
     8701 taaagaataa agaaaatgca tcaggaacaa aattataatc tgccacctga ctcacaccgc
     8761 ttttaaagta aggggcatca aaatcaaaac cgcaaaaaaa ataatttttg cattgatttt
     8821 taatagattt aaaattcgaa tatagtgttt ctccagattt aaaaagttta tctttatcta
     8881 tatatttgat gctttcccta tccaaaataa atatttcaaa cattaaaaaa tcattacatg
     8941 accaagccaa atcataaatt tgattcaaat gggtatcata aagataaaaa taataaggtt
     9001 tgggaacagg taaaatattt aatggaaaag gaggactaat tttcttaata tctgatgact
     9061 gatatccata tctttctata ttcttaaaaa tttcatcttt cttaagataa caagacattc
     9121 tgacaatcac aacctgttca tcggatatat tatctatttg catggcgcgt ataacacgcc
     9181 atgcctgatt aaaattagtc tcccttacct tagaatctat tatctttttc aaagatgaaa
     9241 atgcccctta tcttgaactc tccagttaga acctggaagt ttcgtacctc ttaaatgtgt
     9301 attaatattt cttatagttt cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt
     9361 cgtaccgctt tcttcaaatt ccaggtattc ccccattagc taacgcaaag aagcagacgc
     9421 catttcggct tcgttatgat aaacccgcct tccgttgata tagacttccg cccctgtccg
     9481 ctccaaattc caaaaattcc gtactgaaat ttccatatcc cgatattgtg cagaccatgt
     9541 tttttcgaag gttttcataa aatttcctat acctgtccaa tcggcacata tcaattgcat
     9601 tattatagtg gattaacaaa aatcaggaca aggcaacgaa gccgcagaca gtacaaatag
     9661 tacagaaccg attcacttgg tgtttcagca ccttagagaa tcgttctctt tgagctaagg
     9721 tgaggcaacg ccgtactggt ttaaatttaa tccgctatac atctcaatac gataaatatt
     9781 ccttaagtca aaatgcaagc ctgaccgtac cttaactgtc aaaattttat tattttttat
     9841 tgattttaaa acaatttctg caaaattctc ttcgctttct ctctttttta gaagcacata
     9901 agaaaaaata aaacttcccc gattaaattc ataaatatgt ttcaaccatt cgcctcctct
     9961 ttctgtaaga caagattcag tttcattctt ccttattgta taaatatttc cttcacaaaa
    10021 tctgaaataa atccataaat ccatcttatc cataattaaa gaaaaagttt cacctcgaga
    10081 ttttgtcaac aattcgtaag gttgcgatgt tgcaatcaaa tagccgaaag acatttttta
    10141 cctcatacat ggtcgaaatc agtttctgtt agttcagaat ccattttttc gtcaacaact
    10201 gaatccgcat ttttgaatta acgttttcat cagctgccgt ttatctaaac cggcaggttc
    10261 agtttcaaaa taagccttat atgaagactg taagcatttc agaaaaagat catcagaaga
    10321 catatctgcc gaatcaaata caactgtttt gattttggta cttacccaaa accctttttg
    10381 ctctttttct actatacgga aattcagaat atttccaacc gaatcaaaag cacggtaaac
    10441 atcatccatc aaatcctgcg gctctatttt cttttccaat tccgacaatc cttgaaatat
    10501 atccaaagac acatcttcaa atagaaaaaa aggaggagtt agaagcggtt tttccatgat
    10561 ctgtccgtag attttgattc ccaagggcga tgacgaccaa ttccctgtcc aggcaaagtc
    10621 ttgcccgtat tatccgtaac tcgacgatga taatggggaa attttccaat aggatgacct
    10681 gttctattac cgaaaggggc tatccgcata ttattgccga ttttaatctc acgtccatat
    10741 ttagcaaagg aaacaacctt tcctgcggcg cctacaccac caggaattgc gcctaatccg
    10801 ccagcaatag caacatctct aacagaagct ggtctgcctg tcgttgcata actaaaacca
    10861 tgctgtgtcc acataccaat ggcagcacca cccaagatag ccaatggaag aaacgccctc
    10921 tcagtctcct tcatctcctt ttgagaaag
//
LOCUS       DAWKOD010000053        10925 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000053 DAWKOD010000000
VERSION     DAWKOD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.053"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(81..1226)
                     /gene="dapE"
                     /locus_tag="WJK73_001048"
     CDS             complement(81..1226)
                     /gene="dapE"
                     /locus_tag="WJK73_001048"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ7557541.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQQLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVGKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGSSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1340..1960)
                     /locus_tag="WJK73_001049"
     CDS             complement(1340..1960)
                     /locus_tag="WJK73_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ7557542.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(1994..2503)
                     /locus_tag="WJK73_001050"
     CDS             complement(1994..2503)
                     /locus_tag="WJK73_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7557543.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIARYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2597..3148)
                     /gene="azu"
                     /locus_tag="WJK73_001051"
     CDS             complement(2597..3148)
                     /gene="azu"
                     /locus_tag="WJK73_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ7557544.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKITLVD"
     gene            complement(3429..3851)
                     /locus_tag="WJK73_001052"
     CDS             complement(3429..3851)
                     /locus_tag="WJK73_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ7557545.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3982..6732
                     /gene="secA"
                     /locus_tag="WJK73_001053"
     CDS             3982..6732
                     /gene="secA"
                     /locus_tag="WJK73_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ7557546.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGVRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTNQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6876..8648
                     /locus_tag="WJK73_001054"
     CDS             6876..8648
                     /locus_tag="WJK73_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7557547.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8835..10763
                     /gene="rpoD"
                     /locus_tag="WJK73_001055"
     CDS             8835..10763
                     /gene="rpoD"
                     /locus_tag="WJK73_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ7557548.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2869 a         3038 c         2685 g         2333 t
ORIGIN      
        1 aatttaatcc actatatctc aaacccacat taggtctaat caaatggtcg gacatccata
       61 tccgacagcc cattttcttt tcagacggca tttccagcca acaagcgcgc caatatcccc
      121 tcatacaccg ccgacagctt cggaatatcg tccagccgca cgttttcgtt gatttggtgg
      181 atggtcgcat tggacgagcc taattcgatg agttcttttg caatggcttt gatgaagcgt
      241 ccgtccgaag tgccgccggt ggtggacaat tcggtctcta cgccgcagat ttcggcaatg
      301 gcggcgcgtg ccacgtcggt cagtttgccc gcgtgggtca ggaagggctg ccccgaacac
      361 gaccactgca aatcgtattg cacaccgtgt ttgtccaaaa tggcgtggac gcgttgtttc
      421 agccctgctt cggtggactc ggtggagaaa cggaaattga atttgacatt cagctcgccc
      481 ggaatgacgt tggtcgcgcc tgtgccgccg ttgatattgg aaatctggaa actggtcggc
      541 gggaagtatt cgttgccttc gtcccagact tcctgcgtca gctctaacaa ggccggggca
      601 aaagtatgga cgggattgat tgccaaatgc ggataggcaa tatgaccttg cttgcctttg
      661 acggtcaggt tgcccgacag cgagccacgc cggccgtttt taatcatatc gcccaatttg
      721 cccacggcgg tcggctcgcc gacgatgcag tagtcaatca actcgccgcg cgctttcaac
      781 acatcgacga ctttggtcgt accgtccagc gcgtcgccct cttcgtcgga agtaatcagg
      841 agcgcgatgc tgccttggtg gtcgggatgt tcggcaacaa agcgttcgca ggcggtaaca
      901 aaacaggcga tgctggtttt catatccgcc gcaccgcgcc cgtataatct tccgtcgcgc
      961 tcggtcggct cgaacggggg cgaatcccat ttttcaacag ggcctgtcgg cacaacgtcg
     1021 gtatgccctg caaaacagac gacgggaact ttcgtgccgc gtcgcaacca gatgtttttg
     1081 gtgtcgccga aatggagttc ttcagccgca aaaccgattt tgtgcaggcg ttcggccagc
     1141 agttgttggc agttttggtc atctggcgtc acggatggtc gggaaatcag ctctttggca
     1201 agctctaggg attgagtttc ggtcatattt gttcactttt gaaattagac cgtctgaaac
     1261 gttctgaatg tgattttcag acggcattta ggttaggttg gcatacgggg tgggtatttt
     1321 tacccgtcag tcttctgaat catttgccgt ggcaggcttc gtaaagcggc agcaaatctt
     1381 ccaccgtttc cgctatccat ttcgcgacat cctgcctgcc caaatcgtcg cgttcgatgt
     1441 gtttgccgat gcagaaaaag tcttcgtcgt tttgcaactt tcggtcggac tcgttttgtt
     1501 gcgcgacggt gcggtagtcg tcgtattcgc tttccgcacc gtgccacata tcgaaagaag
     1561 cgtatttttc ggtatcaaaa ttatccaacc agcggttgta atcaggcagc gcgatgggcg
     1621 acacgtcggc tttgtagcag tgccaatcca agctgacgct caaacggcgg cggttgagca
     1681 gtatcgacaa aatcgcggcg gaatttttat attgttcgta tttgaagtag gcaaagaaat
     1741 gggcgcgaac ctgccagccg ttacaccagc gttcgatgtg cggaggcgca aacggcgcac
     1801 ccaattcggc ggcaacctgc tgaatcagct gctgccatat ctgccagttt tctttatagt
     1861 cagccttgat ttgcggaatg ctttcaggct ggtatttttt aagctgggaa aattggaaaa
     1921 acgggatatt gaacaaatcg caacttttcg gggtcagcat aatatatcct tgagacgatt
     1981 gtttcagacg gcattatttg cgccggcgcg ccgccataat ttcgccgatt tcggtcagtt
     2041 tttcttttgg gataaaggtg ttgcccatat caaacagcgg ctcttcaatc gccagatgca
     2101 catcgtaacc tgccacaaaa cgtttgaacg cttcctcatc ggggacataa gcgttgtctg
     2161 cttcgagttt ggcaaattcg gcagccacgg catcccagtt gccatacagg ctcacatgct
     2221 gacgcaaaag ctcgtccacg ctttcttggg cttgcggcgc gtattgcagc agcagcggaa
     2281 agaagttttc ttcttcgtct tcatggtgca gcggcgcggc aacgttgaaa taccgggcga
     2341 tttggcggat ggtttgcaaa acaatctgat tgcagccgtt ttcggcgata tagtccgaca
     2401 gcatggcgac ttgtccgcaa aaacggcgta ctttgccatg gcaggcatac agcatttcaa
     2461 tcggttcggc aaaggtaacg cttttggttt caaacggatt catggttttt tagattttat
     2521 tcttaaatta acgtttccag attataacaa aacagcctgc tcaaggcagg ctgtctgttt
     2581 tcaagcttta ggcgaattag tcaaccaaag taattttacc gttcatcaaa gcaccgtgac
     2641 ccgggaaggt gcaggcaaat ttgtagtcgc cgtcagccaa tttggcagga tctagagtca
     2701 gggaagactc ttcgccgccg ccgatcagtt tggtgtgggc aacaacgcgc gcatcgtcag
     2761 gtttaacgta gtcagtgtca gctgcgccaa cgccatcttt gaaaataccg tccatgtctt
     2821 cagttttacc gatgacaatg ttgtgaccca tgctggtttt aggttgggta ccggtgtgtt
     2881 tcagggtgat ggtgaattcc ttacaagctt tgcttacttg gatgtcttta gtgttgaact
     2941 gcatattgtc gttggattcg acagttgccg cacagttgcc ggcagcaggg gcttcggcag
     3001 catctgcagg agcagcttcg gcggcaggcg cttcggaagc gggtgcttca gcagcaggag
     3061 ttgcctcggc agcaggcgcg gcaggttctt gagagcaggc agccaaaccg ataacggcgg
     3121 cagaaatcag agccagatac gctttcataa caaatctcca atcgataaaa taatattcgg
     3181 ttttacagaa atcaaagtgc aaccgccatt aacaaaacct tgaaaaagat tccgccgcgt
     3241 tgcacaaaca gatgtttcgg agcggcattt tgctacaaat ttcatttgaa atcaaagcct
     3301 gtctgcaagt ttacaatcgt ttactttaaa aggggcaatt ttaccccgaa cctatttctt
     3361 tagtattaga cccattatcc tttacttctt aatattaacg gatgtttaca caaattcccg
     3421 tatacatttt atgcgccatg ccttctaacc aagtttgcca atgcctccgc caattcggga
     3481 tgccgttttt ccaactttac cgccgccgaa ccgaaactct ccagcgcagc cttactcaaa
     3541 tgcagggtat tggttttcgg cggtttttcc ggtttcgggg ccagcctgac cgaaacagag
     3601 cgtatcgaag catcaagccc tgccaactgc ggcaataccg acggtgcaat cattttcaag
     3661 cgcgatgccg ccatattgtt tgccgccaaa aggacaagcc tgccgtcttc gatacatgcc
     3721 gtctgaaaat gcgggtgcag gttggcaggc agcagttttt tcacggcggc atccaaccgc
     3781 cgccactgtc ccgcctgttt caaaagtccg gaaagcagcg cgtcccgcct gcccaactgt
     3841 tccaaattca taaaacatac acccaaaaag attgaaatac cgcaaacgcg cctttatttc
     3901 agacggcatt agcactttgc acaaacgctt gtgttaaaat cgcgttttcg cccactatta
     3961 tatcaggcgc aggaattatt catgctgaca aacattgcca agaaaatctt cggcagccgc
     4021 aacgaccgct tgctgaaaca ataccgtaaa tccgttgcca gaatcaacgc gctcgaagaa
     4081 cagatgcaag ccctaagcga tgctgatctg caagccaaaa ctgccgaatt caaacaacgc
     4141 ctcgccgacg gtcagacttt ggacggcatt ttgcccgaag ccttcgccgt ctgccgcgaa
     4201 gcgtcccgcc gcaccctcgg tgtgcgccac ttcgacgtgc agcttatcgg cggtatggtg
     4261 ctgcacgacg gcaaaatcgc cgaaatgcgt accggcgaag gcaaaaccct ggtcgccacc
     4321 ctcgccgtct atctcaacgc gctggccggc aaaggcgtgc acgtcgttac cgtcaacgac
     4381 tacctcgcct cgcgcgacgc aggcattatg gagccgctct acaatttcct cggcctgacc
     4441 gtcggcgtga ttatttcaga tatgcaaccg ttcgaccgtc aaaacgccta tgccgccgat
     4501 atcacctacg gcaccaataa tgaattcggc ttcgactacc tgcgcgacaa tatggttacc
     4561 aaccaatacg acaaagtgca gcgcgaattg aattttgccg ttgtcgatga agtggattcc
     4621 atcttgattg acgaagcgcg cactccgctg attatctccg gccaggcaga cgacaacatc
     4681 cagttgtacc aaatcatgaa caccgtcccg ccccacctcg tccgccaaga aaccgaagaa
     4741 ggcgaaggcg actactgggt agatgaaaag gcacatcagg tcatcctgag cgaagcaggt
     4801 cacgaacacg ccgagcaaat cctgacccaa atgggattgc tggcagaaaa cgactcgctc
     4861 tattccgccg ccaatatcgc cctgatgcac caccttatgg cggcattgcg cgcgcattcc
     4921 ctcttccaca aagaccaaca ttacgtcatc caagacggcg aaatcgtcat cgtggacgaa
     4981 ttcaccggcc ggctgatgtc cggccgccgc tggtcggagg gtctgcatca agccgtcgaa
     5041 gccaaagaag gcgtggaaat caaacgcgaa aaccaaaccc ttgcctccat caccttccaa
     5101 aactatttcc gcctgtacac caagctctcc ggcatgaccg gcacagccga taccgaagcc
     5161 ttcgagttcc aaagcatcta caacctcgaa accgtcatca tcccgaccaa ccgccccata
     5221 caacgcaaag acttcaacga ccagattttc cgttccgccg aagaaaaatt cgaagccgtc
     5281 gtcaaagaca tcgaagaatg ccacaaacgt gggcagcccg tcctcgtcgg caccaccagc
     5341 atcgaaaact ccgaactggt ctcccacctt ctgcaaaaag ccggactgcc gcacaacgtc
     5401 ctcaacgcca aagaacacga acgcgaagcc ctgattgtcg cccaagccgg caaagtcggc
     5461 gcgattaccg ttgccaccaa tatggcggga cgcggtacgg acatcgtttt aggcggcaac
     5521 ctgaagcacc aaaccgatgc catccgtgcc gacgaagcct tgagcgacga agagaaacag
     5581 gcacaaatct ccgcactcga agacggctgg caggcagaac acgacaaagt gatggaagca
     5641 ggcggtttgc acatcatcgg tacggaacgc cacgaaagcc gccgcatcga caaccagttg
     5701 cgcggacgtt ccggccgtca gggcgacccc ggatccagcc gtttctacct ctcgtttgaa
     5761 gacccgctgc tgcgcctatt cgcactcgac cgcgccgccg ccatcctcaa ccgcctcgcc
     5821 cccgaacgcg gcgtcgccat cgaacacaac ctgctgacgc gccaaatcga aggggcgcaa
     5881 cgcaaagtcg aaggcagaaa cttcgatatg cgcaaacagg ttttggaata cgacgacgtt
     5941 gccaacgaac agcgcaaagt catttacagc cagcgcaacg aaattctgac cagcaaagac
     6001 atcagcgacc tgatgcagga aatccgttct gatgtcgtca gcgacctcgt ggatacctat
     6061 atgccgcccg acagcatgga agaacaatgg gacatcccga ctttggagaa ccgtctggct
     6121 gccgaattca gactgcacga agacatccaa tcctggctga aggcggacaa tgcgattgac
     6181 ggtcaagaca tcaaagaacg cctgatcgaa cgcatcgaaa acgaatatgc cgccaaaacc
     6241 gaactggtcg gcaagcaggc aatggccgat ttcgagcgca acgtgatgtt gcaggtcatc
     6301 gacaaccaat ggcgcgaaca cctcgccgct atggactacc tgcgccaagg catacacctg
     6361 cgcagctatg cccaaaaaaa tccgaagcag gaatataaac gtgaagcctt taccatgttc
     6421 caagacctgt ggaacggcat caaattccat attgcctccc tgcttacctc ggttcaaatc
     6481 gaacaaaacc ctgtcgcggt ggttgaagag caacccatcg gcaacatcca gtccattcat
     6541 tccgaatcgc ccgatatgga agaacttttg ggtcagtcgc aaaccgatct ggttaccgaa
     6601 gcctttaatc ccgatgggac agatttcagc cccgaagcct tggaagcgcg ggggcaaatc
     6661 gtccaccgca acgacccctg cccctgcggc agcggtttga aatacaaaca atgccacggc
     6721 aaactggctt gagcgtttga acgcaaatgc cgtctgaaca tccggtttcc ggcttcagac
     6781 ggcattttgc ctgaatcgcc gtatccgacc gacaatccga aaactcctga tttgatacca
     6841 tccgaactaa aaacaggcat ccgccccgcc gaactatgat tccatccgac ttcattgacg
     6901 aacttctggc caaaaccgat attgtcgaca tcatcgacga acaggttccg ctgaaaaaag
     6961 gcggggcgaa ctatatggcg tgttgcccgt tccacaagga aaaaacgccg tcgttttcgg
     7021 tcagtccgac caagcagttt taccactgtt tcagttgcgg ggcgcacggt tcggcgattg
     7081 gttttgtgat ggaacatcag gggctgtcgt ttccggaggc agtgcagttc cttgccgacc
     7141 gcgtgggtat gattgtgcct aaagtgcgcg ggcagaacga taatcccgaa gtccgtgccg
     7201 aacgtaagaa aaagcagcag acgctggaag aaacgacagc ggcggcggct gatttttatg
     7261 cgcaacagtt gaaattcaat ccgacggcga aggcttattt ggacaagcgc ggcttgagcg
     7321 cggaagtcat cgcgcattat ggtttgggct acgcgcccga cggctggcag cctttggcgc
     7381 aagtattcca accgtacccg aataccgcgt tggtggatac gggtatggtg attgacaatg
     7441 aggggcggta ttacgaccga ttccgtcatc ggattatgtt ccccatccgc aatccgcgcg
     7501 ggcaggtcat cggtttcggc ggcagggtgc tggacgactc caaacccaaa tatctaaact
     7561 ctcccgatac gcctttgttt gataagggga aaaaccttta cggattgtat gaagggcgtg
     7621 ctgctgtcaa ggaagtgggg cggattctgg tggtcgaagg ctatatggac gtggtcgcgc
     7681 tggcacagtt cggcgtgggc tacggcgtgg cggccttggg cacggcaaca acagcggaac
     7741 acgtcaaaat cctgatgcgt caggcggaca gtatttattt ctgtttcgac ggcgacagcg
     7801 cggggcgcaa agcggcttgg cgcgcgctgg aaaacgcgtt gccgcagttg aaagacgaca
     7861 aatcgctgca ttttttgttc ctgcccgagg aacacgaccc cgacagttac atccgtgcct
     7921 acggcaaagc gcaatttgaa gacgcgcttc tgaatcaaag caagcctttg tcggagtatt
     7981 tctgggaaca cctttcagac ggcattcatc tcaatacgca ggaaggcaag gcggaattgg
     8041 tgaaaaccag ttcgccgctt ttggcgcaga ttaccgcgcc ggcattggct tatttgttaa
     8101 aacaacggct tagcgaactg gtcggcatcg accccgacaa cctcgcgcaa ctgctaggac
     8161 aggaagcgcc gaagcggcac gtcaaacaaa aaaactacaa actgcctccg atttccgtca
     8221 aacagcccgt catgctgacg ctggtacagc ggcaaatccg cagcctcttg ataaatccgg
     8281 attgggctgc atatatagac ctgcccgatt atctggcgtt ggacggtgat ttcgcctgcc
     8341 ttgccaatct tgccgaatcg attaaaaacc atgccgccgt acccgaaacc gctcaggttt
     8401 tagagtatat gcgcggctcg ccttacgaag aaacgataac ccgaatcttc cattcaacgc
     8461 accaatcgga agaaatgaac agcagcagtg aagaagattg cgagaatttc caaatcggca
     8521 tgaaaaaact gctcaatgag ttaaaataca gccaaatcga agcattgaaa caaaaaagcc
     8581 tgcaatccgg cttaaatgaa agcgagaaaa aacttttgct gtcgttgctg accgcaaaac
     8641 aaaattgacc ggcggattcc gccacccgta aacccgttat gccgtctgaa aagcacttac
     8701 cccggctgca acaacgacac ctgcagaaca cccatcccaa aaagccttca gacggcatca
     8761 gagtacccgc tctgccacgc cttcaggtgc gttcaaacgc aaaccgtcgg caccttacca
     8821 acagaaagca gacaatgtcc agaaaccaaa atcacgaaga atatcaagac gacacccgtc
     8881 cgttaagcat tgaagagcaa cgcgcgcgcc tgcgtcagct catcatcatg ggtaaagaac
     8941 gcggctacat cacctactcc gaaatcaacg acgccctgcc agacgatatg tctgatgccg
     9001 accaaataga caatatcgtc agcatgattt ccggtttggg catccaagtt accgaacacg
     9061 cccccgatgc ggaagacata ttgttaagcg acaatgccgc cgttaccgac gatgatgccg
     9121 tcgaagaagc cgaggccgcc ctttccagtg cagattccga gttcggcaga accaccgacc
     9181 ccgtccgtat gtatatgcgc gaaatggggc aggtcgacct gctgacccgc gaagacgaaa
     9241 tcatcatcgc aaaaaaaatt gaaaacgccc tgaaaaatat ggttcaggcc atctccgcct
     9301 gcccgggatc cattgcggaa atcttagaac tcatcgaaaa aatccgcaaa gacgaaatcc
     9361 gcgtcgacga agtcgtagaa gccattatcg acccgaatga agtattgctc aacgaattgg
     9421 gcttggggca cttggaaacc acagcgcccg agaaaccttc caacgacaat tcggacgaaa
     9481 acgaagacga cgaagaatcg gaagaagatg cggatgaaat ctcggcagcc aatctcgccg
     9541 aattgaaaca aaaagtcatc ggccactttg cccaaatcga aaaagactac aaaaaaatga
     9601 ttggccgttt ggaaaaacac cacagccggc acaaagacta tctcgcctac cgcgacgcga
     9661 ttgccaacaa actgctggaa gtccgtttcg ccacccggca aatcgacagc ctcagcagca
     9721 gcctgcgcgg gaaagtagaa aacatccgca aactcgaacg cgaaatccgc gacatctgcc
     9781 tcgaccgcgt ccatatggaa cgcgactact tcatccaaaa cttcctgccc gaaatcacca
     9841 atctagaatg gattgaagaa gaaatcgcca aaggcagggt ttggagcgac gcgctcgacc
     9901 gcttccgcca cgccatcctc gaaaaacaaa ccgagttggc ggatatggaa aaagaaaccc
     9961 gcatttccat cgaagagttg aaagaaatca acaaaaatat ggtgtcaagc gaaaaagaaa
    10021 ccgcagccgc caaacaggaa atgattcagg caaacttgcg cctcgtgatt tccatcgcca
    10081 aaaaatatac caaccggggc ttacaattcc ttgatctgat tcaggaaggc aacatcggtt
    10141 tgatgaaagc ggtcgataag ttcgaatacc gcagaggcta taaattctcc acctacgcaa
    10201 cctggtggat ccgccaggca attacacgct cgattgccga tcaggcgcgt accatccgca
    10261 ttccggtaca tatgattgaa accatcaaca agatgaaccg catctcgcgc caacaccttc
    10321 aagaaaccgg cgaagaaccc gattccgcca aacttgccga actgatgcag atgcccgaag
    10381 acaaaatccg caaaatcatg aaaatcgcca aagagccgat ttcgatggaa acccccatcg
    10441 gcgacgacga cgattcgcac ttgggcgact tcatcgaaga tgccaacaat gttgcgccgg
    10501 ccgatgcggc aatgtacacc agcctgcacg aagtaaccaa agaaatcctc gaaagcctga
    10561 caccgcgtga ggcaaaagtc ctgcgtatgc gtttcggcat cgatatgaac accgaccaca
    10621 cgctggaaga agtcggcaga cagtttgacg taacgcgcga acgcatccga caaatcgagg
    10681 caaaagcact ccgcaagctg cggcatccga caagaagcga ccgtttgaga agtttcttgg
    10741 acagcgaaga cagcaagctg taaaccaaaa aaccgcaggt ttcaaatacc tgcggttttt
    10801 tcttacacaa taaacaacgc ttccacatat cccacactcc tatcccgaga cctttgcaaa
    10861 atcccccaaa atcccctaaa ttcccaccaa gacatttagg ggattttcca tgagcacctt
    10921 ctttc
//
LOCUS       DAWKOD010000054        10779 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000054 DAWKOD010000000
VERSION     DAWKOD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.054"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..305
                     /locus_tag="WJK73_001056"
     CDS             123..305
                     /locus_tag="WJK73_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557549.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(367..1368)
                     /locus_tag="WJK73_001057"
     CDS             complement(367..1368)
                     /locus_tag="WJK73_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7557550.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1444..5277)
                     /locus_tag="WJK73_001058"
     CDS             complement(1444..5277)
                     /locus_tag="WJK73_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ7557551.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAIFD
                     VHTLDSDGLNVVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELADIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKTLRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQIIHFINRPLPKNVPAKTPRSLLGIEDDKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPTDYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5538..5634
                     /gene="ffs"
                     /locus_tag="WJK73_001059"
     ncRNA           5538..5634
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WJK73_001059"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5801..7492
                     /gene="dld"
                     /locus_tag="WJK73_001060"
     CDS             5801..7492
                     /gene="dld"
                     /locus_tag="WJK73_001060"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ7557552.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7624..7731
                     /locus_tag="WJK73_001061"
                     /pseudo
     CDS             7624..7731
                     /locus_tag="WJK73_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7787..8158
                     /locus_tag="WJK73_001062"
     CDS             7787..8158
                     /locus_tag="WJK73_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ7557553.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8313..8861
                     /locus_tag="WJK73_001063"
     CDS             8313..8861
                     /locus_tag="WJK73_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7557554.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8996..10087
                     /locus_tag="WJK73_001064"
     CDS             8996..10087
                     /locus_tag="WJK73_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7557555.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10545..10715
                     /locus_tag="WJK73_001065"
     CDS             10545..10715
                     /locus_tag="WJK73_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ7557556.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2510 a         2956 c         2908 g         2405 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt ccttatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtaatc ggcttttgct cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgtcgg cgcattcggt atgcaggaag acgatacccc gcaatcggat tatgaaaaac
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatacgcc ggatacggaa gaaaagcagc
      301 aataaagata tgaaatgccg tctgaaagat tttcagacgg catttgcttt gaagcatggg
      361 agacagtcaa acatttatct catgctcaaa tgcgttcagg attcggcgga cttcatccgc
      421 atccttaacc gttcggacgc ggtcaaacag cgtttgcgcc gggtcgaacg tttttttcat
      481 catgcccaac cattgcttaa gacgggcgat cgggtattta ttgtttgcct cttttgtcag
      541 gcacagctcg aaaaactgcc gtatccattt ggaaacttcg gcaaaatccg tgtctttgac
      601 cggcccgccg ttctcgtatt gcttgatttg ccgcgccaaa tcggggcgga tgaccgcgcc
      661 gcgaccgagc atcacgctgt tgcagccgct gattgttttg atgccgatat agtcttgcag
      721 gctgaaaacg tcgccgttgg cggtaacggg aatattgacg ctgtctcgga ttttccttat
      781 ccattcccaa tgcgccggcg gttcgtaacc ctcggctttg gtacgcgcgt gtacggtcag
      841 tccgcatgcg cccccttcgg caatcgcaca ggcgcattcc aaagcccggc ttttgtcttc
      901 gtaaccgagc cgcatttttg cggtgagcgg aatatgtgcg ggcaaacgtc cgcgcagcgt
      961 tttgacgatg tggaatatca gttccggctc ttttaaaagg attgcgccgc ctttgtgttt
     1021 gttgacggtg ggcgcggggc agccgaagtt caaatcgatt ttgttcgcac cgaagcggac
     1081 ggcttccagc gcattcgccg ccatattgtc cgcatcgctg cccaaaagtt ggacggtgca
     1141 gggcgtgccg gaaaacgttt tgtttccgtt ggcgatttcg gggacatatt ttaaccatat
     1201 ggatcgggaa tgcacggtat gggtaatgcg tacaaattcg ctgacgcatt cgtcgtagcc
     1261 gccaatacgc gtcagcaggt cgcgcatcac gtcatcgacc agaccctgca taggggcaag
     1321 gatgattcgg gttttttgtt tgggttcggt tgtttgtccg tcaatcatga tttgggtagg
     1381 tattgggttt aaacaatgcc gtctgaagac ttcggcgttc agacggcatt tccgtgtccg
     1441 ttgttacagc aatacttttt ccacgccgcc gttattggct tttttcacaa actcgtctag
     1501 ccagttttcg ccgaggatgt gtttcgccat ttcgataacg atgtagtcgg tgggcatatt
     1561 gttgtcgtcg gcgtagcggc tcaagccttg cagacacgcc ggacaggagg tcagcatttt
     1621 gacgggttcg ccctgcggca gctctttgag gtttttctca atttcctctt gtttgcggaa
     1681 tttgacttgg gtggcgatgt cggggcgttt gacggcaaac atgccggatt cgccgcagca
     1741 gcggtcgctt aagacgactt tctgccccat caggctgctg gccatttggg tggcgttcat
     1801 ggttttgatg ggcgtgtggc aggggtcgtg gtagaggtat tgctgacctt tcacgccgtt
     1861 cagtttcacg cctttttcga gcaggtattc gtggatgtcg atgatgcggc agccggggaa
     1921 gatttcttcg aagcggtatt tttcgagctg gtcgtagcag gtaccgcaac tgacgacgac
     1981 ggttttgatg tcgaggtagt tgagggtgtt tgccatacgg tggaaagcca cgcggttgtt
     2041 ggtgctcatt tcttcggctt ttgccttatt gccgccggcg tcctgcggat agccgcaaca
     2101 catatagccg ggcggcagga cggtttgcac gccgacgtgc cagagcatgg cttggacggc
     2161 gagtccgatt tggctgaaca ggcgttccga accacagccg ggaaagtaaa acacggcttc
     2221 ggcatcttcc ggagaagccg gattgcggat gatggggatg cttttgtcgt cttcgatgcc
     2281 caataaggag cgcggtgttt tggcaggcac gtttttgggc agcggacggt tgatgaagtg
     2341 gataatttgt tctttaatcg gggctttgcc gacggttgcc ttgggttcgg ctttttgctt
     2401 tttcgtgccg acgggcagca gtttgccgat tttgtaggcg aaattctgcg cggggaagcc
     2461 ggtttgtatc atcgcggcac gtagggtttt aatggttttc gggccggtcg cgtttaggaa
     2521 tgccataccc attgaagctg ccggcgcaaa gcgtttgtgg ccggaatcgg caaggtagtt
     2581 gcggacggct acggttacgt cgccgaagtc gatattgacg gggcagggtt tgacgcagcg
     2641 gtggcacacg gtgcagtggt cgccgatgtc tgccagttct tcaaaatgtt tgacggaaac
     2701 gccgcgacgg gtttgttctt cgtataaaaa ggcttcggtc aagagcccca cgccgaggat
     2761 tttgttgcgc gggctgtaca gcaggttggc acgcggaacg tgggtggagc agacgggttt
     2821 gcatttgccg cagcgcaggc agtctttgac ggaatcggca atcgtgccga ggtctgattt
     2881 ttccataatc agcgattccg cgcccaaaag ctcgaaagac ggcgtatagg cgttgcgcag
     2941 gtccgagcct ttcatcagtt tgtggcggtt gaaggtgtgt ttgggatcga cttggttttt
     3001 gtagtcccaa aacggctgca tttcttcgtc gcttaaaaat tcgagcttgg taatgccgat
     3061 gccgtgttcg ccggaaatca cgccgttaag cgaacgggcg attttcataa tgcgttccac
     3121 agaacggtat gccgtctgaa gcatttcggc atcgtctgaa ttgacgggga tattggtgtg
     3181 gacattgccg tcgccggcgt gcatatgcag ggcgacaaag actctgccgc gcacggtttt
     3241 ggcgtggatt ttgcccaagc cttcgatgat tttggtgtcg gttttgccgc tgaagatttc
     3301 agaaagcggc ttcattacgt ctgctttgac agacacgcgc aggcggaaat cgcggaatgc
     3361 ggtaaagcag ctttcattgt ctttggcttc gggcgcggcg tggacggctg cgccgtagcg
     3421 ggccttgtag tcggcaagcg gcgtgtcgag attggcgagc aaccagtccc aacgcgcttt
     3481 gacggcggaa acgtgggcca gggcgtgttt gccgcgttcg cccaacagtt cggcggtcgg
     3541 caggtcggtt cccattttgt cgatggggag tttgccggac agatattgct ccaaggcggc
     3601 acagagtttg agtttgtttt gaatggaaag ctcaatgttg atgcgttcga tgccgtctga
     3661 atactcgccg agcctttcga gtgggatgac cacgtcttcg ttgattttaa aggcgttggt
     3721 gtgtttggcg atggcggcgg tgcggctgcg gtcgagccag aaggttttgc gggcttcggg
     3781 agatatggcg ataaagcctt cgccgtcgcg ggcgcgggca agttcgcaga tgtgttcggc
     3841 ggctgcctct acggcggctt cgtcgtctga aaccacgtcc gccagcaaaa ccattttcgg
     3901 ccgtcccttg cccgccgctt tggtggcgta gccgacggcg cggacataac gccagtccaa
     3961 atgttccaaa cctgccagcc gtacgctttc atgggcaagc aaaaagtcgc ggatttcgac
     4021 aatagatggc gtggcggtgg caaccgtgcc gaaaaactcc atacacacgg tgcgcgtgta
     4081 tttcggcatt ttatgcaaca cgaaggcaac gctggtgatg atgccgtccg tgccttcttt
     4141 ctgcacgccg ggcaggccgc tcaagaattt gtcggtaacg tctttgccca aaccgacttt
     4201 gcggaatttg tgaccgggaa tttccaagcg ttcggttttg acgacattga ggccgtctga
     4261 atccagtgtg tgaacgtcaa atattgcggt ttcttcgtcg tggattttgc cgaaattgtg
     4321 gcgcacgcgt tcgatacgca gccattcgcc ttgagggttg accatttgcc agtaggcgag
     4381 gttgtccaac gccgtccccc acagcacggc ttttttaccg cccgcgttca tcgctacatt
     4441 accgcccacg caggacgcgt cggcagaagt cggatcgacg gcgaacacca aacctgcctg
     4501 atgcgcggtt tcttccactc gccgcgtaac cacgcccgcg ccgcaccgga taatcggatg
     4561 tctgccatcc aagcccgcca gttcgacaaa ttccacgccg cgatgtttgt ccagcttttc
     4621 cgtattgatg accgcgctca ttgcgtccaa aggaatcgcg ccgccggtat aacccgtacc
     4681 gccgccgcgc gggataataa ccaagtccaa ctcaattaag gcgcgcacca aaggcgcgac
     4741 ttccgcctcc gtgtcgggat tgacgacgac aaacggatat tcgacgcgcc agtcggttgc
     4801 atcggtaaca tgcgttaccc gcgccagccc gtcgaacata atattgtgcg gcttggtaat
     4861 tttgcttaag cgttccaaaa tttgatggcg tttttggctg gtttcatcaa aactgctatc
     4921 aaaacgctcg actgcctttt ccgctgccgc aaccaataca tccacttgcc gattatcgtc
     4981 gcggcgtttg cggatttcgt tcaaacggtg gcgcatttcc cgcaccagcg cggcgcggcg
     5041 tttcgggtgc tccaacaagt catcgaccag atatggattg cgcacaacta cccaaatatc
     5101 gcccaacact tcaaacagca tccgtgccga acgtccggtt ttacgctgtc cgcgcaagtc
     5161 ttgcagaatc tgccacgctt cgtcgcccag caggcggatg acgatttcgc ggtcggtata
     5221 agaagtatag ttatagggaa tttctcgaat acgctgaggg gcagtagtcg tggtcatggt
     5281 gtgtccgtgc ttggcagttt ttatttgaat gccgtctgaa aattcggttt caggcgagtt
     5341 tgaaaaggcg ataatgtagt tcaatttttg atatttttca atgccgaaac aggaaatacc
     5401 cttatagaaa caaaataacg ctttgagaaa ttgttgacaa tattatcaaa tataaatttt
     5461 aggtggtgtt tccggcgtat gaccgtatca aaacggttga gtttttcaga aaacccgtta
     5521 taatgccgtt tctctgaggc gggtctcccc gcatggcaaa tcggaacacc gggtcagggg
     5581 cggaagccag cagcccactc cggtgcgcca gtgccggggg tttggcccgc cgccctattt
     5641 gaaacgccgg agctttgatg ttccggcgtt cccgtatcgc accgttgccg tctgaaagcc
     5701 gttcagacgg cattttcttt actttgccat aacgtataac tacaatgtga aaacaattcc
     5761 aatgtgcaat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     5821 gtcaagactg acccaaaccg taggcgaaaa atacatcatt accgaccccg cgaaaaccga
     5881 acaataccgc caaggttacc gcttcggcga gggtaaggcg ttggcggtgg ttcgccccgg
     5941 aagcattctg gaaatgtgga aaattctgca ggcgtgcgtc gaagcagacg tgattgtgat
     6001 tacgcaggcg gcgaataccg gactgaccgg cggttcgacc cccgacggca acgattacga
     6061 ccgcgacatc gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacaacaa
     6121 cgaacaagtc gtctgcctgc ccggctcgac cctgaaccag cttgaattgc tgctgaaacc
     6181 cttgggacgc gaaccgcatt cggttatcgg ctcttcctgc atcggtgcgt ccgtcttggg
     6241 cggcgtgtgc aacaactccg gcggcgcatt ggtacagcgc ggcccggcct acaccgaaat
     6301 ggcactgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     6361 agacttgggc gacacgcccg aagaaatcct gaccaacctg caaggtcatc attatcaaaa
     6421 caaagacatc aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg
     6481 ccaagtggac gaaccgaccg ccgcgcgttt caatgccgac cccgcccgcc attacgaagc
     6541 ctcgggctgc gcgggcaaac tgatggtttt tgccgtccgt ttggacacct tcccgcaaga
     6601 aaaacaaacc gccgtgttct acatcggcac gaacgacatc aacgagctga ccgacatccg
     6661 ccgcgccgcc ttgggcgaat ttgaaagcct gcccgtttcc ggcgaataca tccaccgcca
     6721 cgctttcgat attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     6781 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     6841 agccagcttc ctgcccaaac atttttccga caaggccatg cagttcgtca gcaaatacct
     6901 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     6961 tctgaaaatg ggcgggaaag gcgtggatga ggcgcgcgcg ttcttaaaag aatattttgc
     7021 acaccacagc ggcgcgtttt tcgagtgcaa cgccgaagaa acccaagccg cgatgctgca
     7081 ccgtttcgcc gtcgcctccg ccgccatccg ctaccgcgcc gtgcatgatg acgaagtgga
     7141 agacttggtc gcgcttgaca tcgccctgcg ccgcgacgac cgcgactggt ttgaaaaact
     7201 gccgcccgaa atcgacaata aaatcatcca caaactctac tacggacatt tcatgtgcca
     7261 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga
     7321 aatgctgcat cttttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     7381 tttgtatgaa gccaaacccg cgctcaaaca gttttaccgc aaacttgacc cgaccaacag
     7441 cttcaacccg ggcgtaggca aaaccagcaa aaagaaaaac tgggcggaat aagcgtgtcc
     7501 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     7561 ttgaagttcc ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct
     7621 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     7681 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     7741 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     7801 aatctacacc catcccgccc tgccgcccca agccgtcaaa caaggctggt catggccggg
     7861 gctcttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     7921 gacgggcgcg atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc
     7981 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     8041 caacagcctt tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt
     8101 tgaggcagcc aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc
     8161 gcgttttctt ttccatcgtg ccgtctgaaa aatgttcaga cggcatttct tataaccttt
     8221 cgtttgcagc acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc
     8281 ccgtacccaa tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg
     8341 aaaccgcccc cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta
     8401 tccccaacct tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag
     8461 tcggcaagct caacgccgcc aacagcctga ccgccggcga agtcgaagta atccagatta
     8521 ttgccgcccg caccaaccaa tgcggcttct gcgtggcagg gcacaccaaa ctcgcaaccc
     8581 tgaaaaaact cctttccgaa caatccgtca aagccgcgcg cgctttggcg gcaggcgaat
     8641 ttgacgatgc taaactcggc gcgctcgccg cctttaccca agccgtaatg gcaaaaaaag
     8701 gcgcggtatc cgacgaggaa ctcaaagcat tttttgatgc gggctacaac cagcagcagg
     8761 cagtcgaagt cgtgatgggc gtagccttgg caactttgtg caactacgtc aacaacctcg
     8821 gacaaaccga aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat
     8881 atcgggcggt ttttcaaaaa caccccctca aacaaaaaca agccgcctaa gcggcaggga
     8941 agcagccgcg catcaggcgc gcccccgatt tccccgcaaa ccttaaggaa caaagatgaa
     9001 cgcccaaacc ctgattgcca acgttgccga attcgtcaaa accaagctca aacccatagt
     9061 ggacgacatc gaccgcaaag gatactaccc cgaagcattt atgcgcgaac tcggcgcaat
     9121 cggtggattc ggcgcagtcg gtacagaagc cgaaggcggc aacggcttgg gtttggcaac
     9181 gcaaatcgcc gtattgcgcg aaatcggcaa agaatgcggc gcaacctctt tcagtgcgtg
     9241 gtgtcaggcg gcttgcgcgt ggtatctgca ccaaacgccc aaccgggccg tcaaagacaa
     9301 ataccttgcc gacatcctgc aaggcaaagt attggcgggt acgggcatgt ccaataccgt
     9361 caaacacctt gccgacatcg aaaaacacaa cctccaagcc gagcgcgtgg agggcggcta
     9421 cacagtcaac ggcgcgctgc cgtgggtgtc caacatcggc gaagaccaca tttgggcgaa
     9481 taccgcccaa atcggcggcg gctacgtcat gttcatcaca ggcggacaat gggaaggcgt
     9541 aagcctgcaa aactgccccg aattttgcgc cctcgaaggc acacgcacct tcagcctgaa
     9601 cttcaaagac gtatttatcc ccgacgaaga catcatcgcc gcgcccgaac agtttgccga
     9661 ctacatccaa agcatcaaag caggctttat cctcctgcaa atcggcatcg gcgcgggcgt
     9721 gatagacggc agcctcggca tcatccgcct cgccaacgtc gtcaacgccg aagtcaaccg
     9781 ctatctcgac gacggctacg acagcctcaa aacaaggctg gacggcgcgt gggcagaaac
     9841 cgaacggctc gccggattgg cttggagcgg cacgcccgac aaccttacca ccctcaagct
     9901 gcgcgaagcc gccgccgtac tgacacttgc cgccgcccaa tccgccgccc tgcattccgg
     9961 cgcaaaaggc tacctgatgc ggagtcccgc ccaaaggcgc gtccgcgaag caatgtttgt
    10021 cgccatcgta actcccgcga tcaaacacct ccgcaaagaa atagcggcaa tcgaagccgc
    10081 aaaataaaca ggcggaaaaa accgaaaccc ccatcgcgtc attcccgcga aagcgggaat
    10141 ccagccccca acgtaacagg aatccatcgg aaaaaacaga aaccctcaaa ccgtcattcc
    10201 cgcaaaagcg ggaatccagc cccccctaac gcaacaggaa ttcatcgaaa aaaccgaaac
    10261 ctccaaaccg tcattcccac ggaagtggga atccagtaat cgaaaaacca caggaatcta
    10321 tcgaaaaaaa accaacccca aaaaaaccgg gcggataccg caccattctc ccaaaccctg
    10381 atttatagcg gatgaacaaa aatcagggca aggcggcgaa gccgcagaca gtacaaatag
    10441 tacgggtgct tcagtacttt agagaatcgt tctcttcgag ctaaggcgag gcaacgccgt
    10501 accggttttt gttaacccac tatacaaaaa acaaggaaac acaaatggcg caatatatgt
    10561 gcggcccctg cggctggatt tacgatgaag aacacggcga ccccgaacac ggcatcgccc
    10621 ccgggacaaa gtttgaagac atccccgacg actggaaatg ccccgaatgc ggcgtgggca
    10681 aagaagattt ctacctgttg gatttcgtga tatagcggca gccgcaccct tagggaaaac
    10741 cccgtcatcc ggcgttccgc ctgcggcggc agggaacac
//
LOCUS       DAWKOD010000055        10293 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000055 DAWKOD010000000
VERSION     DAWKOD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.055"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..1859
                     /gene="mafB"
                     /locus_tag="WJK73_001066"
     CDS             33..1859
                     /gene="mafB"
                     /locus_tag="WJK73_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ7557557.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPVPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSNNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            1862..2326
                     /locus_tag="WJK73_001067"
     CDS             1862..2326
                     /locus_tag="WJK73_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557558.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            2515..2718
                     /locus_tag="WJK73_001068"
                     /pseudo
     CDS             2515..2718
                     /locus_tag="WJK73_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2709..2996
                     /locus_tag="WJK73_001069"
     CDS             2709..2996
                     /locus_tag="WJK73_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ7557559.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNKLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            3492..4748
                     /locus_tag="WJK73_001070"
     CDS             3492..4748
                     /locus_tag="WJK73_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7557560.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            4779..5198
                     /locus_tag="WJK73_001071"
     CDS             4779..5198
                     /locus_tag="WJK73_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557561.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            5235..6209
                     /gene="mafB"
                     /locus_tag="WJK73_001072"
                     /pseudo
     CDS             5235..6209
                     /gene="mafB"
                     /locus_tag="WJK73_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            6220..6681
                     /locus_tag="WJK73_001073"
     CDS             6220..6681
                     /locus_tag="WJK73_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557562.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            6707..6955
                     /locus_tag="WJK73_001074"
     CDS             6707..6955
                     /locus_tag="WJK73_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7557563.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            6982..7401
                     /locus_tag="WJK73_001075"
     CDS             6982..7401
                     /locus_tag="WJK73_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557564.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            7479..7562
                     /locus_tag="WJK73_001076"
                     /pseudo
     CDS             7479..7562
                     /locus_tag="WJK73_001076"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7559..7801
                     /locus_tag="WJK73_001077"
     CDS             7559..7801
                     /locus_tag="WJK73_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ7557565.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            7879..8124
                     /locus_tag="WJK73_001078"
     CDS             7879..8124
                     /locus_tag="WJK73_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7557566.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            8127..8513
                     /locus_tag="WJK73_001079"
     CDS             8127..8513
                     /locus_tag="WJK73_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557567.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            8635..9576
                     /gene="mafA"
                     /locus_tag="WJK73_001080"
     CDS             8635..9576
                     /gene="mafA"
                     /locus_tag="WJK73_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7557568.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            9580..>10293
                     /gene="mafB"
                     /locus_tag="WJK73_001081"
     CDS             9580..>10293
                     /gene="mafB"
                     /locus_tag="WJK73_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7557569.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT         3333 a         2195 c         2289 g         2476 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggag cttgccgagc gcagcggcca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctacga cgggccacag ggcggcggct
      481 atcccgttcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccatca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcaacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt caaactggca gaccaacgcc
     1261 acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt cgagaaaaat gttcaggaaa taaggaacgg taataaaaac agtaacttta
     1501 gccaacatgc tcaactagag agggaaatta ataaactaaa atctgccgat gaaattaatt
     1561 ttgcagatgg aatgggaaaa tttaccgata gcatgaatga caaggctttt agtaggcttg
     1621 tgaaatcagt taaagagaat ggcttcacaa atccagttgt ggagtacgtt gaaataaatg
     1681 gaaaagcata tatcgtaaga ggaaataata gggtttttgc tgcagaatac cttggcagga
     1741 tacatgaatt aaaatttaaa aaagttgact ttcctgttcc taatactagt tggaaaaatc
     1801 ctactgatgt cttgaatgaa tcaggtaatg ttaagagacc tcgttatagg agtaaataaa
     1861 aatggcaatt tggaattatc ggtactacct tatcccatca gctgctatca gaaataaatt
     1921 taatgcaaat aaagatatca tacttgatga gtatcgatct aatggttttc agaattttaa
     1981 tgagaataaa agttttgaaa attactttat cgataatgat gttatattat tatcaataat
     2041 aaatgaagca aaaaaacagc ttaaattaaa agagtcttgg gataaagacg caatcatgtt
     2101 ttgtgataat tttggtaata gtcttaccgt ttggccagat gatatagagt gcgaacttga
     2161 tttaagattt gattatacta aatttattca gaaaaccatt gattgggcaa taaaatataa
     2221 ttgtctactt gtaatagaaa aaacaggaaa tgtagtttcc cctaatataa ataatctgat
     2281 gtatgaaata aaagcatatt tggaaagcaa gccgtggccc atatgaaacc taaactcaac
     2341 aagtaggatg tgtgcggaac gcacgtatgc ggttctcaag gtttgagcta agaggccgtc
     2401 tgaaaacaga aaaactgttt cagacgacct ttcttttaac cagttgccac agcaaccgga
     2461 caaaagcagc ctacctccac atccatatag gcaatacagg ggagatattt tgtaaattct
     2521 acgaatattt tacctgctaa acagggtagg atatggtatg aagcgaacat tggcttaata
     2581 aacactatgt caagatcgaa tcaggctggt actagattgt tgtattccaa tgatggattg
     2641 ctatatataa caactgatca ttatatctct gcaacacggt ttggagcttg gaaatgggaa
     2701 tataacttat gcaattagag attatcggta gtaaaattta tacggaacaa gattttcata
     2761 atcaaatttc aaaaatattt tctatacaag attattatgg gaacaatctt gatgctctgt
     2821 gggatttatt aagcacaaat gtagaacgac cgattacttt ggtatggaaa gatgctatgt
     2881 tctcaaaaaa taaattagaa aatatattta ttgaaatcgt aaatgttcta gaaagagtta
     2941 agaaacaaga tgaggattat ggattcgaag aaaaatttaa ttatatttta gagtaagtaa
     3001 accctaatta cattacgtac acaggcttaa aactccccag agccaattaa gcaagccgta
     3061 acccatataa aacttaaact caacaagtag catgtgtgcg gaacgtacgc atgcggttct
     3121 taaagtttga gctaagaggc cgtctaaaaa cagaaaaacc gtttcagacg gtctttgttt
     3181 aacgccaccg atccagcggg ttacaaagcg cagtcaatgc cgctgcgcct tatgcctccg
     3241 aagcaatagg cagaacattt ggacacggtg aaaacaaaaa cgaaaccgcc caagccgtcg
     3301 gacatttcct tttaggagca gctattgccc gcgtcaacgg tggtaatttt gctgccggcg
     3361 gctcggcagc agttgcagct gaaaaggcgg cggaacatct tgcccaacag tataacgacg
     3421 gtaaaaccgc aatcgatccg caaacaggcg agttcaatgc caacctgctg ccggaacata
     3481 tcaaagagga aatcaaatca aagagcgggg tgattgcatc gctgacgggc gcggccgtgg
     3541 gcggcacgcc ggtagatgcg caaaccggag gtgcggtcgg acagaatgcg gtggaaaaca
     3601 acctctatct gacatcggaa gccttaaaga aggacaagca gacagctcgt aaaatttatt
     3661 ccgtcataaa agagcaagtc aagcatgaat gcagttccac aggaagaatt accgaatgtc
     3721 gtcaaaatat aggacgcatt atcgaattta cccaagacaa acgctttgac agtaggttta
     3781 aggacttaaa aaaagaatcc ttatattacc taaataaaca tcctgattta gtagcctctt
     3841 atttgaaggc tgaatacgaa aagctggata gggaagacaa aagtatcctg caccgctaca
     3901 tctcacccgg ggctgaaatc gtttcgggca gtttgggggt tgttctttca ggagtagccg
     3961 gaggcggatc ttgtgccgag actttcggct taggctgtgc cgccgctttg gttggtgtaa
     4021 cgtcttccta cgatcatgtc attactggga cgaaaaactt cggaaaaaaa gccagcgagc
     4081 aacgaccgac gattgcggtt caggccttga agcagttggg gctgtcggag caggctgcgg
     4141 aatatgttca gttctctata gatttgttca gtgtgggtaa atcggggggc ggtataccta
     4201 aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt tgataggaag cttaataaat
     4261 tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac gagaagaagg agtcagagta
     4321 gtcagtttaa agcccatgcg caacgagaat gggaaaataa aacagggtta gattttaatc
     4381 attttatagg tggtgatatc aataagaaag gcacagtaac aggagggcat agtctaaccc
     4441 gtggtgatgt acgggtgata caacaaacct cggcacctga taaacatggg gtttatcaag
     4501 cgacagtgga aattaaaaag cctgatggaa gttgggaggt gaaaacgaaa aaaggtggga
     4561 aagtgatgac caagcacacc atgttcccaa aagattggga tgaggctaga attagggctg
     4621 aagttacttc ggcttgggaa agtagaataa tgcttaagga taataaatgg cagggtacaa
     4681 gtaaatcggg tattaaaata gaaggattta ccgaacctaa tagaacagca tatcccattt
     4741 atgaatagta atatttatga aaaattagga gattaatgat gaaaagaatt aagtgctttt
     4801 gtgataaatt tccatcagga gatacattta gaatgtgtat cattctggat gactatgata
     4861 atagggttga ttattatgta ggaatatatg attacattac gtctacctta atgagcgata
     4921 tttactatcg atccacgatt gatgagcatt tcaagattat agaattaata gaaaataatc
     4981 caaatgaaat ttatgatgat ggcggtggtc aacaattttg cctagaattt catcatgata
     5041 aggtcatttt ttatcacaat gaatttgatg aagaagatgg ttatccagta ttaagctgtt
     5101 cgctgcatac ttttaaaact gctttaattg cttggaatgc ttttttgcaa ttgcctaaaa
     5161 gtattcattc ggtggtggag actgtgattg aggaataagc ataattagct taatgaatag
     5221 aatcagcgat atagaaaggt agaatcagat atatcccacc taaaaattac tctccttcag
     5281 caccgctacc aaaaggacct aataatggat atagtgccgg gaatttaagc aaaaaagtcc
     5341 tgactttagt ttacataaac ggcaaactca aattcgggcc aagcagtctg ccgtcttcgg
     5401 cattgaaagc acctgttccc attgtcggca aggctgaggc tccgcccgga aaagctgtgc
     5461 ctgtcggcga agcccgcatt ccttcaaatc caaaagcggt aaaaggaaca accgttaccc
     5521 tgtcgcagga acaacgccta gccaacggag agctgatacc gaaaggctct gtggtaacgt
     5581 ggtcggacaa tgcggtgaag gttgtgcggc ctgatgggaa aaagcggaga tttaatagtc
     5641 gggattggga acagaaggca ttgccgaaac cgcaactacc gaaagattac agagatgaca
     5701 tcaacatagg tggaaataat cctagattta atcagcaacg gcatgaaaaa atcgaatccc
     5761 acaacccaaa cagaatagag gagatacgcc aacgtaatgc cgtttcttct gcagaaaggc
     5821 aggctgatgc attaaaaagg atgcaaacgt ctggccgcac ccgcctgcct gggcagccac
     5881 aaaaaggaaa acccggcgct gctagagaaa tgtcctcttc agacaatccg aatcaaacag
     5941 ccgaggcttt tgcacgtcga tatttaggca ggaaacctac actaatggaa cgagtcgaag
     6001 gtgaaaaatt atacggaggt aaatgtgtgg gctgttggaa agcgcaaatg ccggacggaa
     6061 cctttgtggt gtataggcca gcaggtaatg cttctatgac caagggaact actgcgtctg
     6121 ttgatattaa taacccaaca ttaaataact taaatattaa tttaaaaggc aagccaacag
     6181 agttaaaatt taaattccct gctagataag gatgttttaa tgaatttaga gacagcagaa
     6241 aatattcaat gcgaatttga gtatttagca ttagatggtt attttccaat gcattttgca
     6301 tcgcatggtc aaggaaacaa agattggcaa tttgcagttg aattcatata tagattatta
     6361 atatgtcaat tagcaactct tgaacctatc aactttgaaa cgaaaaatga tattttagac
     6421 ttttgccata atttagcaaa acaaagtcct tttaataatg ataatgaggt gtggtatcag
     6481 ggggaaattg tattaacaaa aaaagggata gatttaataa aggaatatat tccagaggcg
     6541 tttgaagagt ggaatggaaa gaaatttgaa ttaaacattc ctttcattaa aacattaaaa
     6601 aatatatttg ttaattatga ggtagcatgg gatgagaata atccactatt ccccattata
     6661 tctttaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg aaattaaaaa
     6721 gcctgatgga agttgggaaa tgaaaaaagg aaggggcgga aaacctatga caaatcattc
     6781 tatgttccca aaagattggg atgaggctag aattagggct gaagttactt cggcttggga
     6841 gaaaagaaag gaattagaag gtcaaaaatg gagaggtaca agtaaatctg gtgttgaaat
     6901 agaaggattt acggaaccta atcgaacagc ttatccactt tataatagta aatgaagtat
     6961 tgattaaaaa ggtgttttta aatgtataaa ataaaatgtt tttatgatca gtttagttct
     7021 ggtgaattat ttaactactg ccagttttta gataattctt ctaatcaaaa gataaataca
     7081 cgaggaacgg tgtatcaata tattatttat gttttaacag gagaccttta tctacaaaaa
     7141 gacatagatg aaaatttaga atttatccat caagcagaaa ataatcccaa tgaagtttat
     7201 tcaggaggtg gacaaggttt ttgctgggat atttctgctg aaaaagtagt tttctatcac
     7261 aacgaatttg atgaagaaga cggctggcct gatttaagct gttcgctgca tacttttaaa
     7321 actgctttaa ttgcttggaa tgcttttttg caattgccta aaagtattca ttcggtggtg
     7381 gagactgtga ttgaggaata agcataatta gcttaatgaa tagaatcagc gatatagatt
     7441 ggactgcaaa tccacgctta tacgctgtgc catgattaac aggaagagag caacttggat
     7501 gggctagtag ggatggtaag catttaaata tatcaattga tggaaagatt acacacaaat
     7561 gactaattta aaattagatt tctactctga agttattata aaagattctt gccctaatga
     7621 tttgttagaa aatggtgaga ctattaaagg aaaaaaagga gtggtattag gtataagcga
     7681 agaagatggt ataatctatg gctatactat tttacttttt gatataaaat attgtatata
     7741 tatagataag aaatatatta taccgactgg aaaaaaattc tctcgcgatg atttttatta
     7801 gctaatttaa gtaatttaag caagtttttt agatactgaa actggtaatt atgtttatgt
     7861 aagcataagg agttcagatt gaaggattct acagggcaaa tgaggctggc aaccaaggat
     7921 ttggcggaag ccattaaacg aggagaagta cgtagttctg cttttacaac aaagcaacta
     7981 aaggcaattg aaaaaggaga atctaaaata ccggattata cttggcatca tcatcaggat
     8041 actggacgta tgcagcttgt acctgaatgg gaacattcta aaaccggtca cataggaggg
     8101 acggcaatgg ataaaggtaa atgattatgt ggaaaattta taaagaaaat agcaccgact
     8161 taaattttgc cataggcagt atatattgcc aagcaatcaa tcttaccgaa tttaaaatgt
     8221 gggtcgaaaa aatcataagg gaagtggatt tagatgaaat tccaaattat ttttttgatt
     8281 tgatagattt tcaatcacta tacgatctga ttgatattat aggatttgtt cccgaaaata
     8341 acttatcaaa aaatcaggat aatgcattaa ccggtattgc ttttttaagg gggatagatg
     8401 tctacgatcc tcccatttca aaagaaaaag cattaaaagc cttaaagaaa catcctgaaa
     8461 tttatcagag gtttcagcat ttctttccgt ttgtagaact gcccccgctt taaacagtca
     8521 aaatgccgtc tgaaagccat ttccgccgct cagacggcat tttcgcccct tttgtttaca
     8581 aacccttaaa atccatttac actcaaaccc gttcaaaaca aaacaaaccc tgccatgaaa
     8641 accctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac cggcattccc
     8701 gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc atcgtcccgc
     8761 gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc cctttacgtc
     8821 tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc tatcgacgca
     8881 ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag ctaccccgcc
     8941 tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac ttccacatcg
     9001 ctcttaaacg cccccgccgc cgccctgact agaaacagcg gacgcaaagg cgaacgctcc
     9061 gccggactgt ccgtcaacgg catgggcgac taccgcaacg aaaccctgct cgccaacccc
     9121 cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg cggcatcgaa
     9181 gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt attcggcacc
     9241 gtccgcagcc gtaccgaact gcacctctac aacgccgaaa cccttaaagc ccaaaccaag
     9301 ctcgaatatt tcgccgtcga ccgcgacagc cggaaactgc tgattgcccc taaaaccgcc
     9361 gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta cagcgtcggc
     9421 aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacattac cccctacggc
     9481 gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa ccccgatgtc
     9541 ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct gcgcagactg
     9601 acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc cgccctcgca
     9661 gcggacttgg cgcaagaccc gttcattacc gataacgccc aacggcagca ctacgaaccc
     9721 ggcggcaaat accacctctt cggcgacccg cgcggcagcg tctccgaccg caccggtcaa
     9781 atcaacgtca tccaagacta tacccaccag atgggcaacc tgctcatcca acaggcaaac
     9841 atcaacggca caatcggcta ccacacccgc ttttccggac acggacacga agaacacgcc
     9901 cccttcgaca accacgccgc cgacagcgca agcgaagaaa aaggcaacgt tgacgacggc
     9961 tttaccgtat accggctcaa ctgggaagga cacgaacatc atcccgccga tgcctacgac
    10021 ggcccgaagg gcggcaatta ccccaaacct acgggcgcac gagacgaata cacctatcac
    10081 gtcaacggca cagcccgcag tatcaaactc aatccgaccg acacccgcag catccggcaa
    10141 cgcatatccg acaactacaa caacctcggc agcaatttct ccgaccgcgc cgatgaagcc
    10201 aacagaaaaa tgttcgagca caatgccaag ctcgaccgct ggggcaacag catggagttt
    10261 atcaacggcg tcgccgccgg cgcgctcaac ccc
//
LOCUS       DAWKOD010000056        10227 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000056 DAWKOD010000000
VERSION     DAWKOD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.056"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(26..349)
                     /locus_tag="WJK73_001082"
     CDS             complement(26..349)
                     /locus_tag="WJK73_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7557570.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WJK73_001083"
     CDS             complement(337..576)
                     /locus_tag="WJK73_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ7557571.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJK73_001084"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJK73_001084"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ7557572.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRQAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2288..2473)
                     /locus_tag="WJK73_001085"
     CDS             complement(2288..2473)
                     /locus_tag="WJK73_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557573.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(2584..3276)
                     /locus_tag="WJK73_001086"
     CDS             complement(2584..3276)
                     /locus_tag="WJK73_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7557574.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(3273..4016)
                     /locus_tag="WJK73_001087"
     CDS             complement(3273..4016)
                     /locus_tag="WJK73_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7557575.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(4045..4608)
                     /gene="gmhB"
                     /locus_tag="WJK73_001088"
     CDS             complement(4045..4608)
                     /gene="gmhB"
                     /locus_tag="WJK73_001088"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ7557576.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            complement(4605..5173)
                     /locus_tag="WJK73_001089"
                     /pseudo
     CDS             complement(4605..5173)
                     /locus_tag="WJK73_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            5343..6995
                     /locus_tag="WJK73_001090"
     CDS             5343..6995
                     /locus_tag="WJK73_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7557577.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            complement(7054..8295)
                     /locus_tag="WJK73_001091"
     CDS             complement(7054..8295)
                     /locus_tag="WJK73_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ7557578.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            8447..9175
                     /gene="ubiG"
                     /locus_tag="WJK73_001092"
     CDS             8447..9175
                     /gene="ubiG"
                     /locus_tag="WJK73_001092"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ7557579.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            9212..10129
                     /gene="thrB"
                     /locus_tag="WJK73_001093"
     CDS             9212..10129
                     /gene="thrB"
                     /locus_tag="WJK73_001093"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ7557580.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
BASE COUNT         2170 a         2403 c         3073 g         2581 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagatact acgacacaag gacgtgtctt tttgatttcg cttcctacag
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cctctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtccactg cccctatttg acccaataat gatttgggca gaatcactcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatgc gtaccatatt taccagttta
      601 tgtaataaca atgttattac cttgatgagg tagtgtcaat attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 atgaaggtcg gtggggcttt gaggcggctg cgttcttgaa tgagggcggc gaagccttcg
      841 acgctgagcc tgccgctgcc gacataaacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaaa tggttttgac gggggatttt gcttggcact cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccaatcaa ataggcggca gcctgccgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac ggtggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgttggg ggggggatgc agttgctacg
     1621 aatcgctatc ctgtgaattc accctgtcag gagtgcccga atcgtcattc ccgcgcaggc
     1681 gggaatctag gacgtaaaat ctaaaaaaac cattttatag tggattaaca aaaaccagta
     1741 cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcatcaagtg
     1801 aatcggttcc gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
     1861 atccactata tccgataagt ttccgcaccg acaaaactag attcccactt tcgtgggaat
     1921 gacggaatgt aggttcgtag gaatgacggg atgtaggttt ccgtatggat ggattcgtca
     1981 ttcccgcgca ggcgggaatc taggtctgtc ggtgcggaaa cttatcgggt aaaacggttt
     2041 cttcagattt tacgttctgg attcccactt tcgtgggaat gacgcggtgc aggttttctt
     2101 aaccccgcgt tctagattcc cactttcgta ggaatgacgg cggtagattt ggcagatgcg
     2161 gcggattcgg caggtctcaa cccatcctac aatccaccct gaccgccttt tacgaatccg
     2221 ccgccatcct gggaaacgca aaaaaatgcc gtccgaaaac ctttcagacg gcattttcgc
     2281 gggcaaatca gtaaaagact tcgcgccagc ttaagcgttt cataccgcac gtcggaccgg
     2341 gcgggtttcg ggtttcggaa gagcggtgtg aaatgaaacg cggcagacgg cgcgtgtgcc
     2401 gccatcctgc cccgaaccgg cggcaaagcg tcatcgcggt ttgaatcggg cgggcgcgtg
     2461 cctgttccgg cacggatgcc gtctgaaagc ggcggcagcg gtatgtgtcg ccggctgtga
     2521 tgctgctttc cggatgtccg tttgggagga ggtctgaatt ggcggatgcc tgcccgggcc
     2581 gcgtcagcct aaggcgaaaa gttccctgcc gtggattttg agccactgtt tcgcttcggg
     2641 cgtgtagggt gcgaagcggc gggtcagtgc ccagaacgcg cggctgtggt cggcgtgggc
     2701 gaggtggcag agttcgtgta tgcagacata gtcggcaacg tattccggtg cgccgaccag
     2761 ccgccagttg aagcgtatgc ctgtggtttt gcggcacacg ccccagaagg ttttggcaga
     2821 ggtcagcgag gagggggcgg ggaacagttg tgtggtgcgg gcgtggcgtt cgaggcgggg
     2881 aatcaggtaa ctgtgcgcct gccgttccaa aaagtcccgc agcagcgcaa gctgtttttc
     2941 gggtgcgcct tcgggaacac ggatttcaga cggcatcagc aggatttgcg tgtcttgatg
     3001 ggcggtgagg gcaagctgtc tgccgtggaa gaggatggat tcgggcagcc ggttttcggc
     3061 agtttgcggc ggcggtgttt tcgccagtgt ttgccgcagg acggcttcgt tttcatacag
     3121 ccagcggttt agagcggaga cggagaagca gggtgggacg ctgatgcgga cggtatgtgt
     3181 gccggcgggg cggataatca ggtttttctt ggcacggcgc ttgatttcga cggtcagttc
     3241 catgccgtct gaaagggtgt ggacaaaggc ggtcatgcgg tttcagacgg cattttggcg
     3301 gcaaacgggc ctgcgccgga aatgagcggc tgctgcgttt cgatgaggtg ttcgcatttt
     3361 cccatcaatt cggcttcgct gccgcttgcg tgcgggatgg tcggacagat gacgacggtg
     3421 atttcccccg gatatttcag aaaggagttt ttcggccaaa attcgccgct gttgagggcg
     3481 acggggacga tgtccatctc aaacattttc gccatgcgcg cgccgccgag tttgtatttg
     3541 ccgcgttttc cgggcgcaag gcgtgtgcct tcggggaaaa tggtaatcca atagccttcg
     3601 tttttgcgcg ccaacccctg ttttatgagc tgctcgttgg cttcgcggcg gttgttgcgg
     3661 tctatgccta tggttttgac cagtttcaag ccccagccga aaaaggggat tttgaacaac
     3721 tcgcgtttgg caacgtaaac ctgcggcgga aaaatgtcct gaagggcgag cgtttcccag
     3781 ccgctttggt gtttggcgca gatgacggcg gggcggtcgg ggatgttttc cgcgccgatg
     3841 atgcggtatt tgagcccgac gatgtgtttg agcgagaggt tgaggatttt gacccagacc
     3901 cgcgccatct tgtgcgcccc gtctcggaaa ggcgaggcga gcagcataaa gggaaagagg
     3961 aaaatcaggg tggaacagag tatcagccag taaatcaggt tgcggatgat gagcatggtt
     4021 ttgccttgtc ggaatgcggt atgttcagtc ggcttgcggt gcggcgtttt cctgcatgat
     4081 gtattgtgag aaatcgagca aggtatcgaa aacctgtgtg tgttcgggta attcgtgtcc
     4141 gtgttgggag agcgtttttt tgccttttcc ggtcagaacc agcgcgggtt ttccgccgac
     4201 ggcatcgatt gcctgcaaat cgcgcaggct gtcgccgacc agccaggttt ccgaggcttg
     4261 ggcgttgaag cgtccgatga tgtcttcaat catacccggt ttgggcttgc ggcagttgca
     4321 gttgtcggca tcggtgtgcg ggcagaacca gatgccgttg atttcgccgc ctgcctgacg
     4381 gacgaggcgg tgcattttgg cgtgcatttc ggtaaggttt tgaacggtaa aatatttgcg
     4441 cccgatgccg gattggttgg tggcaacggc gacggtgtag cctgcctgcg tcagaaatgc
     4501 caccgcatcc atgctgcctt cgacaggtat ccactcgtca acggatttga cgaagtcatc
     4561 gcggtcctga ttgatgacgc cgtcgcggtc gagaatgatg agtttcatcg cgtttccttt
     4621 gggttggggc gtgtcgggga tgggattata ggacataaat ctagattaaa ttctgctaca
     4681 agatgtttct ctaactccaa tattttatct tgtgatctgc gcttcagtgg tttacaatct
     4741 accccgacat aaatagtcca cggctcaaga ttttttctca ctatagcccc tgctccaaca
     4801 gcaaccccat caccaatagt aacgcctggt aatactgtta catttgttcc taaaattgca
     4861 tgttttccaa ttctaacact agatttttta acattagtat atttagatgg gactgttgga
     4921 ttggttaacc cccccccata aaatcatcgc taccggtaat aattctacac cctgcgctta
     4981 atcctgaaaa atgatccata taaaattctc caccaccaat aatactggta aatgatgata
     5041 tatgaacaaa acttcctaaa atacttttct ctccacaatt aaaaaataca aaatcatcaa
     5101 tttgagaata atccccaatt gataaatttt cttttttaat aatctttgca ccatcgtata
     5161 ttttataatt cactttttag ttcctctaaa agggctcatt gtaacttcac cattttcagt
     5221 gattccatct cgaataatta tactgaaata tcggcagtga tgcgcctgtg ctgcattata
     5281 atgcacgctt tcgataataa ttcgcaacgg ctcaggtatg ccgttttgag gagtggggcg
     5341 ggatggttgc ctatgttttc ctgtttttgt ttgtaacggc ggcgttggtg ctgattatca
     5401 ggtcgcacta ccgctggacg tattttttcg catccgcgct gtttgtcttt ttggcgggcg
     5461 gtatgctgat gttgacggct cagtggcaac gtgccttgaa tttcgcttcg gtctggtttg
     5521 tggtgctgat actgttccac aggctgaaaa tccattatta caaacagccg ctgttgattt
     5581 ccgacttttt gctgattgcc gactggcgga attgggaaac gctgttccat tataaggaag
     5641 cggttatcgg tatggcgggg ctgctggctt tggcggcata tgcggttttc ggctggagcg
     5701 gtgcggattc tttggatgtg ccgtggcggt gggcaggcgc ggttttgttt gcggcggcgt
     5761 tcgtgtcgat gcggcatttt tccaaacatc cgggcgcggt aaagacgtgg ctggattcgc
     5821 tgccggacga cgggcgcgac gtgtttttga acctgccgat gtcctgtcgg gcggtgtttt
     5881 tccaagtacc cgtgttcgag ggcgacgggg aagcgtttgc caggcagatg ccgtctgaaa
     5941 cccggccgtg cggcatgtcg gatgaaaaac ccgatattgt cgttaccctg atggaatcga
     6001 cgctcgatcc gcactgtttc gactttgccg ccgccaagat tcccgatttg aaaatgttcg
     6061 gacggcagga agataccgta ttttcctcac ctttgcgcgt gcatactttc ggcggcgcaa
     6121 cgtggaagtc cgaatttgcg tttttggcgg gtgttccgtc cacggatttc ggcgcgttgg
     6181 caagcggcgt gttttactcg gtcgtaccgc atttgcagac cggttttgtc cgcaacctgc
     6241 gcgaacacgg ttatttttgc gtggcactct cgccatttac caagggcaac tacaatgcca
     6301 aggcagcata tgaccatttc ggctttaatc tgatgttcca gccgcaagat ttgggctatc
     6361 ccgcgccgat gggcaaaaac ctgtggcata tttccagtga ggaaatgatg cagtatgcgc
     6421 ggatgatttt ggaaaaacgc catcccgatt tggaaaacgt gcggcagccg atgttcgtat
     6481 atgtgctgac catgaaggag cacgggccgt atcggacgga taccgacaat gtgtttgatt
     6541 tggatgcgcc cgatttgaat gcgaaaaccg tatccgcgct taacgactac atcgggcgca
     6601 ttgccgattt ggacaaagcg gtggaaagtt tcgaccgtta tctgcacgaa cgcggcaaac
     6661 cctttgtttt cggttatttc ggcgatcatc aggtaccgtt tgagggcgtg tccgtcagga
     6721 agaaatggga ttacgcgcag ccggattatg taacgcagtt tgccgtcagg agcaatattg
     6781 ccggcggatt cgtacagcgg cagaatttcc tcgaccttgc ctttgcaggc ggcgtgctga
     6841 tggaggcggc tggtctggaa gccaaagacg gcttcatgcg tgcgaatatg gcgatgcgcg
     6901 gtttgtgcgg cggagggttg gaagactgcc cgaactggga gttggtcgga aattaccgca
     6961 actatctgta cgacgttttg aaaattgccc gttagctttt tgccgcatgg ctgcaacggt
     7021 tgcgaaaatg ccgtctgaaa actcgttcag acgttatcct ttaaatacgg ggacgagttc
     7081 catttggctc aatacctgtg cggcgatgtt gacgatgccg aaaaggaaga cccaaaccat
     7141 cagccacaag ccgccgtaaa ctttataggt tttgcctgcg ccgaattttt tgcgcgaacg
     7201 gtagagcagc atggcgggga tgatgcctgt ccagacggtt gccgccaggc cgacgtagcc
     7261 gatggcggta acaaagccgg tggggaagag caggcaggaa attagaggcg gcaggaaggt
     7321 cagcgcggcg gttttggtgc ggccggacac gctgtcgttc catttgaaga tgtcggcgat
     7381 gtagtcgaag agtccgagcg ttacgcctaa aaacgaggtg gcgatcgcca tataggaaaa
     7441 cagggacaat attttgtcca tattgccggt ttgggcgaat ttggacaggg tttcaatcag
     7501 gacggagact tgcccttcgg cggcaatcac gggggcgaac tcgttgcgcg gcaggttgcc
     7561 ttggatggcg gtttgccaga ggacgtaaat taccagcgca atcagtgtgc ccgtccagat
     7621 ggatttagcc actttgggcg cgtcgccttt aaagtatttg agcaggctgg agacgttgcc
     7681 gtggaaaccg aatgacgcaa ggcagacggg cagggcggtg gcgacataaa tccagtagtt
     7741 ggtgccggta ggggcttggg tgtcgaagag gacgggcagt ttggcatcgg caatcaggcc
     7801 gccggttgcc caaataaagg ttaataccat gccgccgatg aggacgctgg tgaatcggtc
     7861 gaccaagcgt gcggatgccc atacgcaaaa ggcgagaatg ccgaagaaga cgagttgtcc
     7921 gacggtgagt gaaacattgc cgcctgccgc gctgcctaag cctttggcgg tcaggtcgcc
     7981 gccgacgaag atataagcgt aagtaagcag gtataaaacg aaggcgacgg cgatgccgtt
     8041 gatgatgttc cagctccgtc cgagcaggtc tttaaccatg gtgtcgaagc tcgcgccgtg
     8101 ggggtagtgg gtgttgactt ccaaaatcat caggccgctg gagagcatgg aaaaccaggt
     8161 gtacagcaac acggccagcg agccggtaaa ccatacgccg gatgtggcgg tcgggttggc
     8221 gagcatacct gcgccgatga ccgtgccggc gataatcatc gcgccgccga acagtgaagg
     8281 ggttttggtg ggcatatttt gtctttctgc cagaaaaagc gagccgccat tatgccgtaa
     8341 agtgtaagga tttgtaaggt atttgcgccg cgccgcccga aaaggctttc agacggcatt
     8401 gcgttccata gtataatctt gggttttgga gtgggcggtt cgtcagatgg gagggaaaat
     8461 gtccgacaaa aaatataatg tcgatgaggg ggaaatcgcc aaattcagcc ggattgccga
     8521 caaatggtgg gacaagtcgg gcgagttcaa aaccttgcac gacatcaatc cgctgcggct
     8581 ggattatatc gacggacacg cggatttgtg cggcaaacgg gttttggacg tgggctgcgg
     8641 cggcggcatc ttggcggaaa gtatggcacg gcgcggcgcg gcgtttgtaa agggtatcga
     8701 catggcggag cagtcgctgg aaaccgcccg cctgcacgcg gctttgaaca atgtcgccga
     8761 tatcgaatac gaatgtatcc gcgtggaaga ccttgccgag gcggaaccgc actcgttcga
     8821 tgtggtaacg tgcatggaaa tgatggaaca cgtccccgat cccgccgcca tcgtgcgtgc
     8881 ttgtgccaat ctggtcaaac cggacggcat ggtgtttttt tccaccatca ataaaaaccc
     8941 gaaatcgtac ctgcatctga ttgtggcggc ggaatatctg ttgaagtttg tccccaaagg
     9001 cacgcacgac tggaaaaaat tcatcgcgcc tgccgagctg gcgcggatgt gccgtcaggc
     9061 aggcttggat gtggcggata cgaagggtat gacttaccat gtgttgtcgc aaacttatgc
     9121 cctgtgcgat tcgaccgatg tgaattatat gtttgcctgc cgtccggcgt tctgacggcg
     9181 ggtttgcccg tttttgagca agtgagttga tatgtctgtc tataccagtg tttccgatga
     9241 tgaaatgcgc ggcttcctga gcggttacga tttgggtgaa tttgtttccc tgcagggcat
     9301 cgcgcagggg attaccaaca gcaattattt tctgacgacg acttcgggac gttatgtgct
     9361 gaccgtgttt gaagtgttga aacaggaaga gctgccgttt tttctggagc ttaaccggca
     9421 tttgagtatg aagggcgtgg cggttgccgc gccggttgcg cgcaaagacg gccggcttga
     9481 ttccgttttg gcgggcaagc cggcttgttt ggttgcctgc ctgaaaggtt cggataccgc
     9541 gctgccgacg gctgagcagt gtttccatac cggcgcgatg ttggcgaaaa tgcaccttgc
     9601 cgccgccgat ttccctttgg aaatggaaaa cccgcgttac aatgcgtggt ggacggaggc
     9661 gtgcgcccgg ctgctgcccg tcctgtcgca agacgatgcc gcgctgctgt gttccgaaat
     9721 cgatgcgtta aaggacaatc tcggcaatca tctgccttcg ggcatcatcc atgccgattt
     9781 gtttaaagac aatgtgttgc ttgacggcgg tcaggtatcg ggtttcatcg atttctatta
     9841 tgcctgccgg ggcaatttta tgtatgactt ggcgattgcg gtcaacgatt gggcaaggac
     9901 ggcggacaat aagttggatg aggcgttgaa aaaggtgttt atcggcggtt atgagggcgt
     9961 gcgccccttg agtgccgaag aaaaggcgta tttcccgacc gcccaacgtg ccggctgcat
    10021 ccgtttttgg gtgtcgcgcc tgttggactt tcattttccg caggcgggcg agatgacgtt
    10081 tatcaaagac ccgaacgcgt tccgcaacct gctgttgagt ttggattgag tgcgtccggc
    10141 gtttgacaga aatgccgtct gaaagggttt cagacggcat ttttatggct gattaaaacg
    10201 aaaatgagac gatagccggg tattttc
//
LOCUS       DAWKOD010000057        10094 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000057 DAWKOD010000000
VERSION     DAWKOD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.057"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..200
                     /locus_tag="WJK73_001094"
     CDS             <1..200
                     /locus_tag="WJK73_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7557581.1"
                     /translation="DGIMRKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLICARCRLKGSPDA"
     gene            complement(456..668)
                     /locus_tag="WJK73_001095"
     CDS             complement(456..668)
                     /locus_tag="WJK73_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557582.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(680..961)
                     /locus_tag="WJK73_001096"
     CDS             complement(680..961)
                     /locus_tag="WJK73_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557583.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(999..1262)
                     /locus_tag="WJK73_001097"
     CDS             complement(999..1262)
                     /locus_tag="WJK73_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557584.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1282..1854)
                     /locus_tag="WJK73_001098"
     CDS             complement(1282..1854)
                     /locus_tag="WJK73_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ7557585.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1833..2144
                     /locus_tag="WJK73_001099"
     CDS             1833..2144
                     /locus_tag="WJK73_001099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557586.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(2166..3041)
                     /locus_tag="WJK73_001100"
     CDS             complement(2166..3041)
                     /locus_tag="WJK73_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ7557587.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3086..3376)
                     /locus_tag="WJK73_001101"
     CDS             complement(3086..3376)
                     /locus_tag="WJK73_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7557588.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3388..3567)
                     /locus_tag="WJK73_001102"
     CDS             complement(3388..3567)
                     /locus_tag="WJK73_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7557589.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3681..4058
                     /locus_tag="WJK73_001103"
     CDS             3681..4058
                     /locus_tag="WJK73_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557590.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVSKGAEKKAAENTQVSATHKTA"
     gene            4087..4332
                     /locus_tag="WJK73_001104"
     CDS             4087..4332
                     /locus_tag="WJK73_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557591.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4566..5534
                     /locus_tag="WJK73_001105"
     CDS             4566..5534
                     /locus_tag="WJK73_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557592.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5531..6013
                     /locus_tag="WJK73_001106"
     CDS             5531..6013
                     /locus_tag="WJK73_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557593.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            6103..6606
                     /locus_tag="WJK73_001107"
     CDS             6103..6606
                     /locus_tag="WJK73_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ7557594.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6715..7047
                     /locus_tag="WJK73_001108"
     CDS             6715..7047
                     /locus_tag="WJK73_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557595.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            7044..7454
                     /locus_tag="WJK73_001109"
     CDS             7044..7454
                     /locus_tag="WJK73_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ7557596.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            7469..8191
                     /locus_tag="WJK73_001110"
     CDS             7469..8191
                     /locus_tag="WJK73_001110"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ7557597.1"
                     /translation="MNCCWGLSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPR
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8512..8874)
                     /gene="ssrA"
                     /locus_tag="WJK73_001111"
     tmRNA           complement(8512..8874)
                     /gene="ssrA"
                     /locus_tag="WJK73_001111"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8937..9542)
                     /locus_tag="WJK73_001112"
     CDS             complement(8937..9542)
                     /locus_tag="WJK73_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ7557598.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2450 a         2327 c         2566 g         2751 t
ORIGIN      
        1 tagacggcat tatgcgcaaa gcctgccgca accgtccgct gtcggaagcg caaaccaaac
       61 gcaaccgata tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc
      121 gtaaattccg ctatgcgcgg gcagcctatt tcggactgat ttgcgcccgc tgccgcctaa
      181 aaggcagccc ggatgcctga ttatcgggta tccggggagg attaaggggg tatttgggta
      241 aaattaggag gtatttgggg cgaaaacagc tgaaaacctg tgtttgggtt tcggctgtcg
      301 ggagggaaag gaattttgca aaggtctcag agtgagttta ttttggggcg gcggcaggtc
      361 gggggcaagc ggcgtggggc ttggttgtgg tttttaggtt tgggggtggg taaaaaatgc
      421 cgtacccggc gggcaggctt cgcactctga aggtttcaga cggcgtttgt tttttctgtc
      481 caatcgagga actgccgcca tttttccagc gacatatcgg tccgccgcgt ttcggtatcg
      541 ggtttggctt cccagcggcc tacctgtatg tagtgcttca ccccgacgat ttgcgccaac
      601 tcggcctgtg tcagtttgca gcggttgcgc agggtgcgaa ggttgtaagg cgtgtagccg
      661 agttccatgt cgttgcggtt tattttgttt cgcatatttt tttgactgcc cggcggcagg
      721 tttcggtaag gacggcggca aatcgggctt cgtctgccgg ttttccgtcg aaaaaaataa
      781 aatcgtaaag ggtgatgccg ttttcggaat ggcggtaatg cccgcccggg cattcgctgt
      841 ctgtctgcct gtggattttg gcaatcaggc ggggatagcg gcaatgggta atgaaacggc
      901 ttgcgccgtc ttgcccgata atccattcgg ggtagcggtt gaatagtgcg gctctgttca
      961 ttttgttcgg gggataaagc ccctcgcggg gcttgtggtc aggcaaattt gaatatcagt
     1021 gccaacacgg cggcgatggc ggtaaccagc ccggtggccg ccatcatggg ataccatcgg
     1081 gattcttggg ctatttttac ggattcggcg ttgattttgt gcgcgtctgc aatgattttg
     1141 gcgatttcgg catctacttt tctcagactg gagtgtttca tttcttgttc gattatgttc
     1201 atcgtacttc ctttcgtttt tggcggttgc cgccgcttgt cggatggtag gatgtctgcc
     1261 atgtgtatat attgatacct tttaggtttt attgcaagtg ttttgggcgg cggcttcgta
     1321 tgcttggcgg tggcggcggc tgtaccattc ggcgagttcg cgccgctctt ggaggcggta
     1381 gctgtcggcg gtcaggtagt ggtgcaggct tgagaagccg gcgcgttgga gggcggcgcg
     1441 gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct tttccggcgg tcaggctgat
     1501 gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg gatttgccgg gggtgctgcc
     1561 gtcatagccc agtcgtcgga tggcgttgtg ggcgaatttg atgctgatgt ggtcgggatg
     1621 gggggcgggt ttgcgccgtg ggcggatgcc ggggaaaagg tggatgttgt cgccggtctg
     1681 tgtgtgcagc tctcggagta tttctaccgc ccagtccgac agtgggacgg taaaggggtg
     1741 tttggtcttc atgtcggcgg cggggatgtg ccataaccgg gcggtgaggt cgatgtcttg
     1801 ccagcgggcg gaaagcagtg cggacggacg ggatgacttc cccggtttcc ccatcaatcc
     1861 gcgccgcctc gatttttccg ttggcgcgca gcatttccac cgcctgccgc accgacatca
     1921 accgcttgcc gttgcgcctc atcgcctcca ccaaaaccgc cgaaatcaat ttggcttctt
     1981 ccggtttaag ctccgtcttg cccgcatcgc tgcgccgttt gcgcgtcggc ttgacgctga
     2041 ccgcctccag cttgcggtat agcgtggcaa ggctgatgcc caattcctgc gcctgctgct
     2101 taagatatgc agagcgtgcg ccgcgttcca ttgcttccgc ctgattctcg actgccttaa
     2161 gacgctcaat cattgccgga ttcatcgcct tctcccgttt caccgcccaa ccattccggc
     2221 acattgtctg tcggtgcttc agtcggcagg gcatagcttt cgcgcagttg ctcgcagtcc
     2281 aaaataattt gattgagcgt gccgaccatc tttgcctgat ggctgatccc gtgtgcctca
     2341 ctgtgcgcat taagttggtc gaacaaatct ttcagacggc tcacttgact gcggataccg
     2401 acctcaaggc ttgttaactg catcgccaac tcgctgccta cgtcttccgc cttcggctct
     2461 ctgacaacgg tttgcttctt agccagcttc tcggccagct catcaatttt ggctgttttg
     2521 gttttcatca cttcgtcttt ggcggcgagg ttttcgcggc tttcgcgcag ggcgacgcgc
     2581 agctcgcgca ccgtcattcg gtccacatcg tcaaaggtca tgccgttgac ttcttcccct
     2641 tcggccaaac ccaccagcgt aacgtcttct tcgaccagca gctcaagcag cttcgacttg
     2701 cccaaatcca tcagcttcgg cgcggctttc tgcatttgcg gggtcgcaaa gcggcgagtg
     2761 gctgacatca gacgtgatgt ttctgcgata cccagcccaa attggctttt cacaatttcc
     2821 ataaaccgtc cgtgttccgt atgctctttt aaaataatca gcgcacgtcc cagttcgaac
     2881 atgccttcca tcgtctgccg tactgcttga cgaccgcgtt caacccaacg ctcttcgcta
     2941 taagtctcgc cgttacccca ctgctccatc accagcacac tgcgcatcgc tgcctgattg
     3001 cttactttgt cggttgcgat aatttcaatt tctgtattca ttttttatct ccaaagtttc
     3061 cgacgtcgga aactttcaaa atccgttaat ccacatcgac ccgcttgccg atttcggcaa
     3121 tcttgctttg cagccgttca tgctgctgcc tgaaccgctc tgcgatttgc agggttttga
     3181 tgccgtaggc gtagttgccg ttttcaagtt tgatgaccaa tcccgaggca accaaatcat
     3241 caatatccct gctgacttgc gatggcgtca gccccagtcc gaccgataaa tccttattgc
     3301 tcagaccgat aatcggatgc tcgtcaagcg cgataaagac cctcaatagc cgttgtaccc
     3361 ttttactttc tgccatccgc atcctcctta tttcagtccc agcttcttgg caatttcatg
     3421 ccccttgccg taattgcctt tgcgctgtcc gccgaccacc agatacacat cgcgcggctt
     3481 aaagccgttc tctcttgccc actgcgccag cgtttggccg ttttgggcaa aattttcttt
     3541 tagtttttca acagtatagc catagatagc cctttcccat tgtgataaaa tagtgaacta
     3601 tttatgcact agaaattaat tagtgcatta ccaatgcgtg aattatgaga ttttaaaatc
     3661 tcttttgcaa ggattatttt gtgatttcta aatctctttt tggtaatcgt ttgaaagaaa
     3721 aaagaaagtt tttagggctt acccaagcac aagctgctga aaaagcaggt attgagcgcg
     3781 aaacatgggg caaatacgaa cgtggcgtat ttatgcctag tggagatgta ttgctctctt
     3841 ttttgaatat gggaatagat gtgagcagct tatttgctgc tgaacaggga attaggccgt
     3901 ctgaaaattc tgaaattagt aaggaagagt cagagttatt agggtattac cgccaagcca
     3961 gcgataacgg caaatttgtc attctcagtg tgtcaaaagg cgcagagaaa aaagcagccg
     4021 aaaatactca agtttcagcc acgcataaaa cagcgtgatt tttaaatcca aaatggagac
     4081 agtcttatgt ttgactttga acaacaaatc aaatggggtg agcgagcgga agaaatcgtt
     4141 aaagaggcag ccacccagaa caatatcgaa attcccgaac ctttggcttc ggcattagct
     4201 aaggcggtaa aagtgcatta tctaagccaa gctggtgtgt tttctttggt agaagcctat
     4261 gcagacacgg taaatccaac tgaaaaagaa gtagattacc aagcaatcgg caaagagcta
     4321 tttgaaaaat aaaaaatgcc gtctgaaatt ttcagacggc atttaggaca ctccgacagt
     4381 cgggctgctc cggtgttctg aaacggatat taagcaattg aagcggccgt gcattttaaa
     4441 acgcattaaa aactaccccg acatacaggt catcgggcag gtggttcagg tgttgaaaga
     4501 tttgaattga tgccgtctga atttattttt tcgacacaga tattaaaaga gaaaggactg
     4561 tacaaatggc aaaaaaaact actaaaacta ttcattattt acgtgcgcag caaaaccata
     4621 gcttgttcga tttagaggta gcattaagaa gggttttgtc agctgctcct actgttcaag
     4681 atacacaaat tgaacaatat aatcagctta cccaaatcat gcatagaact ttggatccta
     4741 aagatgtaaa aggcaattca attggtggat tactgataca cattggcagc ggtacaaaag
     4801 atgaacatat ccgcactatg agcaatagac ctgtacagca agatgatcat ggtggaacac
     4861 aagctcctcc aagtggatat tcctttttgc gtaaagaggc ttttttgtat attgttggac
     4921 accatgtaat tttttgcgga catggttttt tgtcagcatc aacggttgct tcttatttaa
     4981 gtttacttag caataaattg agagagaaca atccggatat tgttcaattt aatattgaat
     5041 tcaaagcagt aggcaattgc gacaagttgt cattgataca acaacatggg gtaaaaagta
     5101 ttcttttaga tgcttcggca tatcaattat ccagaaacag actgtatcaa gatagccgtt
     5161 caaccattgc taaagcatta gggaaagttg gtagtgtatt tacaagcgaa ttgagtgatg
     5221 aggaattgga agcacaatcc gaaattcata tcaatttaga agtcttgcta aacggtaatt
     5281 cgagagccag tattgaagcg caatcgctga tgcaggagca ggcagaggaa attattgatg
     5341 atgaaacggt taatcaagga ttttccatta ccacgcaaca aggtgaagtg attcagccct
     5401 ctgatgtaaa actgtctaaa tctgttagaa taggacgata cgatgaagcc aactcgttac
     5461 tccctgactc ggcatttaca gcaatcagtg aatatttcct agaactccaa agcagaaact
     5521 taactgaaca atgaaaaaat atctgccctt actacgtttt ttgttgattg cttgtatatc
     5581 agcattttta gcatggaaag ggcaaccttt tgttcatggg aacgagaagg ccgttgattt
     5641 aattattaac gtatttgcaa tcttagccgg ttttttaatt gccattatga cattgtttag
     5701 tgatatgagg tttgatgaag atgcaaattg gcgacaaatt caaattcgtg aaggcgtaca
     5761 agaacaacgg tatataaaac attccttact tttttacaca tatcttgccg tattagtttg
     5821 tgtttttatt gtcatcttgt tggctcataa agaagaatac aaaaatggac cagccatttt
     5881 ttggcttgaa cggagctatt tatttttagc ctgtatttcc attttttatt ctgtattctt
     5941 acctgggaat ttaattaaaa gccgaaaaga agagtttaaa aaattaatgg aaaagaagaa
     6001 acccaaaatc tagaacgagt tttaaccccc tttaaaatcc cattcagacg accttttcta
     6061 aaatccctgt attgattttt aattcaatac agggattttt ccatgtcaga cgaattcaac
     6121 cgatttatcg aacgcgtcct ctcccacgag ggcggttacg tcaatcatcc caaagacccc
     6181 ggcggcgaaa ccaattgggg catcactaag cgcaccgcac aggcaaacgg ctacaacggc
     6241 tccatgcgcg ccatgacgcg tgaacaggca atcggcattt accgtaaagc gttttgggag
     6301 cgttaccgcg ccgaccaaat gcccgaagcg gtcgcttttc aattttttga tgcctgcgtc
     6361 aaccacggtt acggcaatgc cgcccgtatg ctccaacgcg ccgcaggcgt gccggacgat
     6421 ggaatcatcg gcgaaatcag cctcaaagcc atcaattccc ttcccgaaaa cgacctttta
     6481 ttgcggttca acgccgagcg tctggtcttt tataccaagc tgggtacgtt cacctctttc
     6541 ggcaagggct gggtacgccg tgtggcgcaa aacctgattc acgccgccgc agacaatgcc
     6601 gattgaggag caaaccatgt caaaaaagtc cattctcgcc ctgatggccg cagccatgca
     6661 gcccgatttc agccacagcg acctaggcat tcgctacgcc atgccgactc agggatgttg
     6721 gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa cgcgcagcca aaaaaaagcg
     6781 tcacaaataa ccgccttttt ccgatggctg ggcggcttgg tctctaatcc ggccacaggg
     6841 aaaatcagcc ataccaaact ttgggcaaac gtcgccgcag ccgccatgac ttggaagttc
     6901 gtgcaggcgg cggacgcgcc cgaatggctt tggtgggcat acggcgcgat ggtcggcggg
     6961 tatgcattaa tcaaacgcgg catcgccgtc gtaccgcagt tggcggaagt caaaaaatcc
     7021 gcaaatcagg aaggggggcg gcaatgattg aatttgtccg agccaaaaaa cggctgcttt
     7081 gggcatttgt gcttttgctt gtgtggacgt gcggttaccg atacgccgcc gacaaggccg
     7141 aagcggaaca aaccgccctg attgccacct atcggcattc ttctatggtt gcggcggaac
     7201 aatatgcctt gcagcttaaa aaagcgcagg acgaaaggca gcggtggtac gacttttccc
     7261 aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc gcaaacgaaa aaagccggct
     7321 atctgaaaac caaggaagaa ctgcttgcgg aattggcttg ccttaaagcg gaaatggttg
     7381 ccctaaaaaa gcccgatgcc ttaatccatg ggaaagaagt gcggcagaaa gaacgcaact
     7441 cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt tggggattgt ccttctatta
     7501 ccaattggcc gtccaatcgg cagaagacaa atatgccgat ttgaaacggc atatccatga
     7561 tatttatcga cgacataagg gaagatacgg ctaccggagg attgcggcag ccatccgtca
     7621 cgcaggaaca ccggtcaatc acaagaaagt cagccgtctg atggcgaaga cggggctgaa
     7681 ggcagtgata cggcggcgca aataccgctc gttcaaagga gaagtcggca aaattgcgcc
     7741 gaatatcctg cgacgctgtt tccatgcaga aaagccgaat gagaaatggg taacggacgt
     7801 taccgagttc aatgtaggcg gagaaaagat atacctttct ccgattatgg atttgtttaa
     7861 cggggaaatc gtcagttacc gtattcaaat ccgcccgact ttcgatttgg ccggcgagat
     7921 actgaaaggt gcgccggaga aaccgagatc gtctgaaaag ccgatactgc attcggatca
     7981 aggttggcaa tatcagatgt ttttttatag tggattaaat ttaaatcagg acaaggcgat
     8041 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttaaatt
     8101 taatccacta tacaacaacg atagaatcaa gttgaaatta aaagggctga gccttgttca
     8161 gtacagaatt cagtccctga aagccgcttg attaaactgt ccgacttttt ggggtcagtt
     8221 cggcttcggc atttttttat ccgtttgggg gtaacttgtt tggaaagctg caagcttata
     8281 aataaaggat tacatttaag ttttgggtaa cctttttaaa aaaatgcgtg atgacttttg
     8341 cattcttaag gcgttttttg gggtaattcg tgaaaagagt taccccaaaa gttaccccat
     8401 aaatggcgaa aactcaagca tacgccagca tattcaaggc aacaaaaaag ccttgaaact
     8461 gttgaagttc aaggcttttt tgtgtttcag gatactgctg aaaatagggt atggtggagg
     8521 cggggggaat cgaacccccg tccgaaagtc ctctacaaag cgttctacat acttagttgt
     8581 gtctatttga aaatcttatt tccatcatgc cgaccaacag gccttttgga aaccagttac
     8641 cttaaatctc atttcctgcc aagtaacccg gcaggaaacc agtcaatgta agatgacgtt
     8701 gcggtggctt tcgccacaca gcccattgac cgactgctgc aacggctagc cttaagcggc
     8761 taaagcgtaa gtttcgtcgt ttgcgactat ttgaattcag tgttttacgg gaatctgaga
     8821 ccccggtatg cccgcatctg cttcgcaacc cccgtcgaaa ccaaggtcgc ccccaaaaat
     8881 ggtttgcaaa ttatacggat attgtgcggt gctgccaagt ctgtcggaga aatttgtcag
     8941 tcttgctgcc ttaatttgcg tttgagcagg atgcggacgc agccgtcgtt gccttcccgg
     9001 ggttcggcgt aggcgagtac atcggggtgt tgcatcagcc agtttcgggt catatttttc
     9061 agaacgggtt tgtagccttt ggaacctaat ccgctgccgt ggatgatttc gccgcatacg
     9121 ccgcgttttt gggtgaatgc gatgaattcg ttgaggactt tttgggcttc ttcctgtgtg
     9181 tagccgtgca ggtcgacatc ggtaacgacg ggatagtatc cgtttttcag gcgttggatg
     9241 tcgttttttc cctgtccgtt tttgctgaag ctggcgggcg ggtcgttgta tgtgctgccg
     9301 atgtagaagt agttttcttc gtcggcgcgg ttgtctttgg gacggacttt gatgggggtt
     9361 ttgtcgggcg gcgcataata ttgctgccgg ttttttaatg gggagacttg tccgactgct
     9421 tgtgaaaaat cgaaatcctg ttcttgtttt tgcttgtttt tttcggctgc ctgtttttct
     9481 gccgcttctt tttgggcttg tttgcccagt tgtttgagga tgttttggaa gtcggtattc
     9541 atattttttc ctgttatttg tccgatggct gtttcgggcg gggttttaat ttgccggaat
     9601 gtttgccaat cgggggagga tgattttgtt gccgtcgtat gttttttgaa agtgtgattg
     9661 tatatcaaaa agaaatgcgg caaccgtcgg cagtgttgat tgccggaaat gcgggccggt
     9721 cgaaccgata tgcccgaacg cctgataaag ttttaaaaac ctgccttgcg aagcaggtta
     9781 atggttttgc caattttgaa ttgccggaaa cgcgaaacac ggaaatctga tgttttataa
     9841 atgttccgat acgaactgca aaatattggt tgagaccttt gcaaaattcc tttccctcac
     9901 gacaaccgaa accccaacac aggttttcgg ctgttttcgc cccaaatatc tcctaatttt
     9961 acccaaatac ccccttaatc ctccccggat accctataat caggcatccg ggctgccttt
    10021 taggcggcag cgggcgcact taacctgttg gcggctttca acaggttcaa acacatcgcc
    10081 ttcagatggc tttg
//
LOCUS       DAWKOD010000058        10043 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000058 DAWKOD010000000
VERSION     DAWKOD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.058"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1336)
                     /locus_tag="WJK73_001113"
     CDS             complement(119..1336)
                     /locus_tag="WJK73_001113"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ7557599.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJK73_001114"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJK73_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ7557600.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="WJK73_001115"
     CDS             complement(2348..2812)
                     /locus_tag="WJK73_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ7557601.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="WJK73_001116"
     CDS             3029..3805
                     /locus_tag="WJK73_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ7557602.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4050..4904
                     /locus_tag="WJK73_001117"
     CDS             4050..4904
                     /locus_tag="WJK73_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ7557603.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4945..6153
                     /gene="pncB"
                     /locus_tag="WJK73_001118"
     CDS             4945..6153
                     /gene="pncB"
                     /locus_tag="WJK73_001118"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ7557604.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6245..7963
                     /gene="argS"
                     /locus_tag="WJK73_001119"
     CDS             6245..7963
                     /gene="argS"
                     /locus_tag="WJK73_001119"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ7557605.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8529..9947
                     /locus_tag="WJK73_001120"
     CDS             8529..9947
                     /locus_tag="WJK73_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7557606.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2289 a         2962 c         2555 g         2237 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcacacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcaacgc cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttgtcg aatgcgatac cggttacggc agctctttcc atattgtcgt
      601 cctcttcaaa ggtaattaag gtgccgttgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcaaattcta ctgaacggat ttgcaaaact ttcgagccaa
      721 ggctggccag ctcaatcatc tcttcaaacg ttaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggtggtg taaacgccgt ccacgtcggt atagatttgg cattcgtctg
      841 ctttgagggc tgcggcgagc gcaacggcgg aggtgtcgga gccgccgcgt ccgagcgtgg
      901 aaatgtcgcc ttcgctgctg atgccttgga aaccggcgac gatgacgact ttgccggcgg
      961 caaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagccgg tgtagctttt ggcttccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttat
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccgcctgccg tttgacagcg gtcagcgaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcggac aacgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg taccttggat ttcgatgatt tttcccgatg
     1681 cggtcatgac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgtg cgcgtaccgc catctgcctg aatcacatcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ctacgaccgc gcgtagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tacccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccag atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgcc
     2881 gtttcattat agacccgctg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gcgacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag gatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacgc ggcaatcaag ctgtcggttc agaaaaacca acatcggcac gcctaaaacc
     3901 gtattcacaa cctgctcctt ttcaaaacat ttgcatttaa aagccgttat aatgccgtct
     3961 gaacatctgc ccgaccacat tatgcgtgaa tgtcggcaga ttgttttctt ttgtaaactt
     4021 atattaaaat ccacttaccg attcacgcca tgccgcccat ccctgcccca tccgcaccat
     4081 ccgagcacac tgtcgcatgg gtattcggcc aacccgttac cgatttgccg caggatttgt
     4141 ttatcccgcc cgacgcgctg aaagtcgtat tgggcagttt tcaaggccct ttggatttgc
     4201 tgctctacct tatccgcaag cagaacatcg atgttctcga tattccgatg gtgaagatta
     4261 ccgagcagta tctgcactac atcgcccaaa tagaagccta tcagtttgat ttggcggcgg
     4321 aatacctgct tatggcggcc atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta
     4381 ccgaaaccgt cgaagacgaa gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg
     4441 cttacgaaca gatgaagctg gcggcgcagg gtttggacgc gctgccccga gccggacggg
     4501 atttcgcgtg ggcttacctg ccgctggaaa ttgccgtcga agccaagctg cccgaagtct
     4561 atattaccga cttgacgcaa gcgtggctgg gtattttgtc tcgggcaaaa cacacgcgca
     4621 gccacgaagt aatcaaagaa accatctccg tgcgcgcgca aatgacggca atcctgcgcc
     4681 gtttgaacgg acacgggata tgcaggtttc acgacctgtt caatcccgaa cagggcgcgg
     4741 cttacgtggt cgtcaacttc atcgcactgt tggagcttgc caaagaaggt ttggtcggaa
     4801 tcgtacagga agtcggtttc ggagaaatcc gaatcagcct caatcatgag ggggcacatt
     4861 cagacggcat ttccggcaca cggggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc
     4921 accaaaaatc gggagacacg ccatatgacc ggcatcatac attcgctgct tgacaccgac
     4981 ctctacaaat tcactatgct gcaagtggtt ctgcaccagt ttccgcagac gcacagcctt
     5041 tacgaattcc gctgccgcaa cgcctcgacc gtctatccgc ttgccgacat caaggaagac
     5101 ttggaagccg aactcgacgc gctctgccgg ctgcgcttca cccacgacga actcggctat
     5161 ctgcgctccc tgcgtttcat caaaagcgac tttgtcgatt atctcgaact cttccagctc
     5221 caacgccgct ttgtcgaaat cggcacagac gataaagacc gtctgaacat ccgtatcgaa
     5281 ggtccgatga tacaggcgat gttttttgaa atcttcatcc tcgccattgt caacgaactt
     5341 tacttccgcc ggctggaaac ccctgccgtt atcgaagaag gtgaacgccg gcttcaagcc
     5401 aaagccgcgc gcctcaaaga aatcgccgcc gcacaaaacc ccgacgatcc gcccttcctg
     5461 atttccgact tcggcacgcg ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc
     5521 accctgctcg aagccgcccc aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa
     5581 aaactcggca ttacccccat cggcaccatg gcgcacgaat tcctccaagc cttccaggcc
     5641 ctcgacgtac gcctgcggaa tttccaaaag gccgcgctcg aaagctgggt gcacgaatac
     5701 cggggcgatt tgggcgttgc cctgaccgac gttgtcggta tggatgcctt cctgcgcgat
     5761 ttcgacctct atttcgccaa acttttcgac gggctgcgcc acgacagcgg cgacccttac
     5821 gtttggggcg acaaagccta cgcccactat caaaagctca aaatcgacag ccgcaccaaa
     5881 atgctgacct tctccgacgg gctggacatc gaacgctctt gggcattgca ccaatatttc
     5941 aaagaccgct tcaaaaccgg cttcggcatc ggcaccaacc tcaccaacga tatggggcat
     6001 acgcccttga atatcgtctt gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg
     6061 tccgactctc cgggcaaaac catgaccaac aacagcacct tcctcgccta cctgcgccaa
     6121 gtgttcgacg tacccgaacc cgaaacgccg taaaccggca gaaaaagcgc acaattcctg
     6181 tttctgccgc ataaaatctt ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa
     6241 cttcatgaac ctacatcaaa ccgtcgaaca cgaagccgcc gccgcctttg ccgccgcagg
     6301 catcgccgac agtcccgttg ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca
     6361 aatcaacggc gtgatgggtg cggcgaaaaa agccaaacaa aacccgcgcg aactggcgca
     6421 aaaggtcgcc gacgcattgg caggcaacgc cgtcatcgaa agcgcggaag tcgccggtcc
     6481 cggttttatc aacctgcgcc tgcgccccga atttctcgcg caaaacattc agacggcctt
     6541 gaacgacgca cgtttcggcg tggcaaaaac cgacaaaccg caaaccgtcg ttatcgacta
     6601 ttcttcgccc aatctggcga aggaaatgca cgtcggccac ctgcgttcca gcatcatcgg
     6661 cgacagcatt tcgcgcgtgt tagaatttat gggcaatacc gttatccgtc aaaaccacgt
     6721 cggcgactgg ggtacgcagt ttggcatgtt ggtcgcttat ttggtcgagc agcaaaaaga
     6781 caatgccgcg ttcgagttgg cggatttgga gcagttttac cgcgccgcca aagtgcgctt
     6841 tgacgaagac cctgcctttg ccgacaccgc acgcgaatac gttgtgaagc tgcaaggcgg
     6901 cgatgaaacc gttttggcat tgtggaaaca gtttgtcgat atttcgctct cgcacgccca
     6961 agccgtttac gacacgctgg gcttgaagct gcgccctgaa gacgtggcag gcgaatcgaa
     7021 atacaacgac gatttgcaag ccgtggtcga tgatttggtt caaaaaggct tggcggtcga
     7081 agacgacggc gcgaaagtcg tgttcttgga cgagtttaaa aacaaagaag gcgaacccgc
     7141 cgcatttatc gtgcaaaaac aaggcggcgg cttcctttat gcttccaccg atttggcgtg
     7201 tctgcgctac cgtgtcggca cgcttcatgc cgaccgcctg ctgtacgtcg tcgaccaccg
     7261 ccaagccctg cactttgagc aactctttac cacttcacgc aaagccggct atctgccgga
     7321 aaacgtcggc gcggcatttg tcggcttcgg cacgatgatg ggcaaagacg gcaaaccgtt
     7381 caaaacacgc agcggcgaca ccgtgaaact ggtcgatctg ctgaccgaag ccgtcgaacg
     7441 cgccaccgct ttggtgaaag aaaaaaatcc cgaattgggt gcagacgaag cggcaaaaat
     7501 aggcaaaacc gtcggcatcg gcgcggtcaa atacgccgac ttgagcaaaa accgcaccag
     7561 cgattatgtg ttcgactggg acgccatgct ctcgtttgaa ggcaacaccg ccccctatct
     7621 gcaatacgcc tacacccgcg tgcaaagcgt gttccgcaaa gcaggcgaat gggacgcgga
     7681 cgcgccaacc gttttgaccg aacctttgga aaaacaactg gccgccgagc ttttgaaatt
     7741 tgaaaacgtg ctgcaaagcg tggcggacac ggcgtatccg cactacctcg ccgcctacct
     7801 ctatcaaatc gctaccttgt tcagccgctt ctacgaagcc tgcccgattt tgaaagccga
     7861 aggcgcaagc cgcaacagcc gcctgcaact ggcgaagctc accggcgaca cgctgaaaca
     7921 aggcttggat ttgctgggca tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt
     7981 gcggacaaca gccccgcaat caaccggcgg accatcctta tccgaatctg aaaaaagcgg
     8041 cgcgatacac cgtatccgcc gcctcccaaa atgcgaaaca gacaaacgcc aagcaagcaa
     8101 gcaagcaagc aagcaagcaa gcaagcaagc aagcaaaaaa ttataacccc cttcctgccg
     8161 acgcacgcac tttccgcgcg gcgcgttccc cttttcccgc tcctcaaacc cgccttttct
     8221 tcaggcaggg tttcagcccg cctcctttcc ctgttttcct tccctcaaca cgcgtgcgcg
     8281 ccccctgccg caccgtgccg cactttcgcg cccggacggc atcggtctgt catccggttc
     8341 tctgttttaa atacccctgt ttcagaaaga aattgcagat gtttcaacac acaggacgac
     8401 acataaagca ccgccctatg tgttgccctg atttggaagg ggttgcgccc ctcccaaata
     8461 aagtctgatc ctgccgcccc gaaggacaga tgtccgagtg gcggggtttc aaccgaaaag
     8521 gaaatacgat gaatattcac gccctgctct ccaaacaatg gacgctgccg ccattcctgc
     8581 cgaaacggct gctgctgtcc ctgctgatac tgctggcccc caatgcgatg ttttgggttt
     8641 tggcactgct gaccgccacc gcccgcccga ttgtcaattt ggactacctt cccgccgcgc
     8701 tgctgatcgc cctgccttgg cgtttcgtca aaattgccgg cgtattggcg ttttggctgg
     8761 cggttttgtt tgacgggctg atgatggtga tccagctttt cccttttatg gacctcatcg
     8821 gcgccatcaa cctcgtcccc ttcatcctga ccgcccccac cccttatcag ataatgaccg
     8881 ggctgttgct gctgtatatg ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa
     8941 ccgacttccg gcacattgcc gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg
     9001 gccatttgag ttactacgac cgggggcgga tggccaatat cttcggcgca aacaacttct
     9061 attacgccaa aagtcaggcg atgctctaca ccgttagcca gaatgccgac tttattaccg
     9121 ccggcctggt cgatcccgtc ttcctcccct tgggcaatca gcagcgtgcc gccacgcatc
     9181 tgaacgagcc gaaatctcaa aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg
     9241 ccaatcccga acttcaaaac gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt
     9301 cggtttggga aagcggcagt tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg
     9361 aattgtgcgc ctacggcggt ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat
     9421 ttgcccgctg cctccccaac cgtttgaaac aagaaggtta tgccaccttt gcgatgcacg
     9481 gcgcgggcag ttcgctttac gaccgcttca gctggtatcc gagggcgggc tttcaagaaa
     9541 tcaaaaccgc cgaaaacctg atcggtaaaa aaacctgtgc cattttcggc ggcgtgtgcg
     9601 acagcgagct gttcggcgaa gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt
     9661 actggatgac gctgaccagc cacgccgact atcccgaatc cgacattttc aaccacaggc
     9721 tcaaatgcac cgaatacggc ctacccgccg aaaccgacct ctgccgcaac ttcagcctgc
     9781 acacccaatt cttcgaccaa ctggcggatt taatccaacg ccccgaaatg aaaggcgtgg
     9841 aagtcatcat cgtcggcgac catccgccgc ccgtcggcaa cctcaatgaa accttccgct
     9901 acctcaaaca ggggcacgtc gcctggctga acttcaaaat caaataacaa caatgccgtc
     9961 tgaacgcacc aacagccttc agacggcatt ttgcagacag accgaccctt caagcccact
    10021 tttttcatca tctccgataa att
//
LOCUS       DAWKOD010000059         9978 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000059 DAWKOD010000000
VERSION     DAWKOD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.059"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..1781)
                     /gene="recN"
                     /locus_tag="WJK73_001121"
     CDS             complement(108..1781)
                     /gene="recN"
                     /locus_tag="WJK73_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ7557607.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQKQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WJK73_001122"
     CDS             complement(1966..2568)
                     /locus_tag="WJK73_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ7557608.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPTTVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJK73_001123"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJK73_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ7557609.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJK73_001124"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJK73_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ7557610.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WJK73_001125"
     CDS             complement(4526..5488)
                     /locus_tag="WJK73_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ7557611.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJK73_001126"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJK73_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ7557612.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLFHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WJK73_001127"
     CDS             complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WJK73_001127"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ7557613.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGALLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVLPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7044..7520)
                     /locus_tag="WJK73_001128"
     CDS             complement(7044..7520)
                     /locus_tag="WJK73_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ7557614.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WJK73_001129"
     CDS             complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WJK73_001129"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ7557615.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WJK73_001130"
     CDS             complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WJK73_001130"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ7557616.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2319 a         2730 c         2503 g         2426 t
ORIGIN      
        1 cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc tgagttctgt
       61 ctattgtttt agacttttat ttttagttat tttttaactg attgatttta aaataaacta
      121 tttttcgacg caagttgcag caattctgcc gcatgttgcc gcgtcgtatc ggtaatgact
      181 tctccgccca acatacgggc aacctcttcg acccgttgga tttcatccaa tatactgatt
      241 tcgctgacgg tttgctctcc ctcgctgtgc ttgcgcaccc gccagtggtt ttcgccgcag
      301 gatgcgactt gcggaagatg ggtaacggca agcacctgat gttttctgcc caacgcacgt
      361 aatgccttgc cgaccatttc agccactccc cctccaatac cggtatcgac ctcatcaaaa
      421 atcagggtgg gaacttgggt atattggctg gcaacaacct gtaaggcaag gctgatacgc
      481 gccaattcgc cgccggaggc aactttattc agcggacggg gcggattgcc tttgttggcg
      541 gcaacttgaa attgaacctg ctccaaaccg tgtgccgtcg gcgacgaggg caacaggacg
      601 atgtcgaaac gtgcaccttt catggcaagg tgttgcatat gctcggtcgt ttcgccgctc
      661 aaacgctctg ccgcctgatg gcgcatggca gaaaggatgt gggcagcttc ctgatattcg
      721 gcaaaattgt gggcaacatt atgctcgagc gcgtccaaat cggcggcagc ttgcaggctt
      781 tgcaggcgtt cttcgatttc tgccaacttg gcaggcaact cttcaggctc gatccggtat
      841 ttccgcgcca tccccatcag ttcgcccata cgctgttcct gtgccgccaa ttcattgggg
      901 ttgatgtcgc tgcgacctgc cacatcgcgc atattggcac tgatttcgcc caattcggct
      961 tcgatgcttg ccaacatatt caggctctcg gcaaagcgcg gctcgatgtt ttgcagattg
     1021 gccaatagtt tttgacattg atagatatgg cgttggatgc cgttgtcgcc gtcaatcttg
     1081 cttccgactt cttcggcagc ctgcaacagc tcggcagaat gggcaaggct gtcgtggctt
     1141 tggctgaggg cttcccactc gccttgttta atgtccaact gattcaattc gttaaactgc
     1201 cattccagac gctcttgttc gagtgcaatg ttttctgcct gtttctgcgc ttcctgcaat
     1261 gctttttttg agtttgccca gttttgataa agctgcctga cggtttccgc ctgcgccctg
     1321 ctgcccgcaa atgcgtccaa caattcgcgc tgggcggctt cctgattaag cgaatgatgg
     1381 gcgttttgcc catggatgtc gataagctgg ctgccgacgg ctttgagttg cgccaaggta
     1441 gcggcctgat tgttgataaa gctgcggctt ttgcctttgg catcgataat gcggcggata
     1501 ctgagttctt cttctccgtc gtttaaaagc ccctgttcat acaattctgc ttttaaaaca
     1561 ggtaaatggg aaatatcaaa caacgccgac aactgcgctt cttttgcgcc gctgcggact
     1621 tggctgtaat cggctttatc gcccaacagc agaccaatcg catccaaagt aatggacttg
     1681 cccgcgccag tttctccggt caatacggta aagccgcttt gaaaatccag attcagattt
     1741 tcaacaatga caaaatcacg caaagaaagt gttagaagca tcgtgaggat tccttagcaa
     1801 ctctaccgac aaaagcaata taaattttag atatacgaat tataactaag atttttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggttgtcg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg aggaagttct tccagaccgt ccaagcgcaa
     2101 atcgctgaga aacgttgccg ttgttgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt ataccttcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca gactgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcctg
     2461 cgacagtggc ggcacacaca attcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttacgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccgattttg ccttcacgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccaccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcagca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca atgcgttgcg tccgactgcg ccgccggcgg
     3061 cgaggttgta gccgctttct tcgacaatga ttttcggcca cagcattccg ggggtgtcgt
     3121 acagccagaa gtcgtcggca aggaaaaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcagatgagg acgcgcaggg gtttatctat gccttggcgg tggggaatca
     3301 tggcacgaca ggcttgggta attttgccgt gtgcgcctgt ttcggaggaa tcgagggcga
     3361 tggcgcaggt gtcggggcgg ctgttatagt gttcgagcca gattttggtg cgctcggggt
     3421 cggcaagatc ttgtttgttg aggattttaa gtttaggttt gcctttggaa agttgggcaa
     3481 gcagggggtt ttcgctggag gcgggcattc gcgcatccag catttcaatc accatgtcta
     3541 cgcttttggc gcgctcggcg atggcttttt tcgctttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctttcctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag gctgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tgctcagagg atgtcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcaatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggacttgccc gaaccggaca ggccgctaat caggacgatc ttcattgtgt tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc gaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc aagctgcttc atatactcgt cgtccgcctc gactaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca ggccggaatg cccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccaag gtgcgttgca
     5101 ggtaaatccg caacacgtcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atgacaatag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 tgaccaaaga catggaaata tcgaacaggt ctccgaactg ataacccgtt tcccccgatt
     5281 cgagacggtt cagatattcc gactctgcca aaccgaccac ttggatttgg ttggggtgga
     5341 taaaattcag gtgtccgacc agggcgagga cgggcttgtc cgcctctaca ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccagg caagttgcag tttgtattgg ttgtcgtcaa
     5461 acagccggcg gacagagata ctgggcatat tattcttcag tcaggatggc gcgggcttct
     5521 tccgcagaag aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc
     5581 agtttggaca atacttccaa atgctcgccc gtcgcatttt ccggaaccag caagataaaa
     5641 accagggaaa ccggtttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 aacaatgagg attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg
     5881 cccacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccag ggcaaatctc
     6001 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6061 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6121 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6181 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6241 ttggcggtat tgcagcaaca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6301 gataaccgca cccaatactg ccatcagcag ggtaaagcct ccgtgacaca gcataacggc
     6361 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6421 ttctttgtcc gccattgcat aaaccgtgtc atacgccaga gtccataaca cattggcggc
     6481 aaagagtatc cacgcttgag gcagcacgtt tccggcaacg gcggcaaacg ccatcgggat
     6541 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6601 cgggtaagtc agcgcaagaa acagcgcggg caggctcatc agccaagtca gatgattcag
     6661 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gcgcttcttt
     6721 tttcttgacc ctgccctgtg cgaacggacg gtttttggta cgctcgacag caccgtcaaa
     6781 atcgcggtcg gcaaaatcgt tgatgacgca accggcactg cgcattaaaa acgtaccgat
     6841 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     6901 gtaggtcggc cacagcaaaa gcagcgcccc aatgggcttg tccgcccgca tcaggcgcag
     6961 gtacacatcc aaacggtcgg acagtcgtaa aaataaaggg gatttaggat tcatattgcc
     7021 gcacagcttg aaaaaacggt attttatccg ataaagcgtt tcagttcggg cagaaaatac
     7081 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7141 cgtccgcatc cttcgccgga aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7201 gcttgaaaca gacgctcgcc caaagggcgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7261 gccgaaccta tacggcactc gctccttgcc tcaacaacag ggataccgtc cagcttcaac
     7321 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7381 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggctttcaat
     7441 aaccggctca tcggcaggtc gatgccgtct gaaacggcgg cgggcaagtc gggcagccat
     7501 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7561 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7621 cttgggcgat gtgtgcgtcg cggctgtcgt ggatgtggtt gaacacgagg ctgtgcaagc
     7681 gaatgccata ttgtttgagc gcggcgaaac tgagtaaagt gtggttgata ctgcccagcc
     7741 gtccgctggt aacgaggatg acgggataat cttgttgctg aatataatca atggttaaca
     7801 aatcttccgt cagcggaacc atcaatccgc ccgcgccttc gaccaaaacg acttcgtact
     7861 gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaagcc ctgccgtcaa
     7921 gacgggcagc gagatgaggc gaagctggat aactgaagat ttcaggcatg gtcagccctt
     7981 gtttgtcggc ttcctgcatg ggtttaccca tgattttacg atggacggcg atgtcgtcgg
     8041 caatgttttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8101 acaattgttt tgccaacata ccggtggcga cagttttgcc gatgtccgtg tctatgccgc
     8161 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8221 aagtttggtc aaaacgccgt ctgaaataat atacggcggc atcagataca ccagcctgcc
     8281 gaacgggcgc acccaaatgc cctgcgccac gcaatctgct tgaaaacgcg ccatatccac
     8341 gcctttatcc agctcgatca cgccgatggc acccaaaacg cgtacgtctt tcacgccgcg
     8401 aatatcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8461 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8521 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8581 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8641 gccgatgcac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8701 gcgcccgaat ccggtggcga tttcatcaaa aatcaaaacg atatcaaact cgtcgcacaa
     8761 atcgcgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     8821 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaaca aggcgcggac
     8881 aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg cttttcggat tatcgacaaa
     8941 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9001 ggacatcgcg ttccaagtat cgccgtgata cccgcgccgt accgtcgcaa tattctgctt
     9061 cgccgtcaac ccccgcgcct gctggtattg caccgccatc ttcaacgcga cttccaccga
     9121 aaccgaaccc gaatccgcat aaaaaatacg atccagcccc tgcggcaaaa tcccgaccaa
     9181 caatttgccc agctccaccg ccggctcatg cgtcaaaccg ccgaacatca catgcgacat
     9241 ctgtttcatc tgcgtctcaa ccgcctgatt caaaacggga tgattgtaac cgtgtatcgc
     9301 acaccaccaa gacgacatcc cgtcaatcag ccgcgtgccg tccgccaatt cgataaacac
     9361 cccttctgca cgtttgacag gataaacggg aagcggatcg gtcatggaag tatagggatg
     9421 gagcagatgg gtacggtcga aatcaagcaa tgatgatgtg tgttgatgtt cagacggcat
     9481 aagtttctct cttttcttat tgctgcattc aaacgcaaaa cgcgtattct actccgacag
     9541 accgcttccc acacctttcc atccgtttcg ggcgtaaaac cacgcaacaa atcgtccgca
     9601 gtataagcgc acaccgtttc gcattcccca agcccgattg gaatcagacg acccaacgcc
     9661 caatattgtt tcacacagcc tccgcccaac gcttcaatca gtcatagaca aaacggcaat
     9721 ccatacgata cagatcatag caacagccat cgcaacagcg ttagcaaaat caggggactc
     9781 cgacataggc gcatagcatc taccgatgca cagctcctca ttcggctcta tgaataccat
     9841 acccatcaca aaatccaccg ccaaaaccag gcacggcttc ttatacttat gatagatttc
     9901 caccatcctg ccccatatat cccaaacatt cataccgcat atcccgcagg caacaaattc
     9961 cgattgaagg ttacagcc
//
LOCUS       DAWKOD010000060         9904 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000060 DAWKOD010000000
VERSION     DAWKOD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.060"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            109..933
                     /locus_tag="WJK73_001131"
     CDS             109..933
                     /locus_tag="WJK73_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557617.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQVYKQSHSALTALQTEQVQDSEHSGKMVAKRQFRIGDIAGEHTSF
                     DKLPEGGRATYRGTAFGSDDASGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAASDIK
                     PDKKRHAVISGSVLYNQAEKGSYSLGIFGGQAQEVAGSAEVETANGIRHIGLAAKQ"
     gene            1073..1750
                     /gene="tsaB"
                     /locus_tag="WJK73_001132"
     CDS             1073..1750
                     /gene="tsaB"
                     /locus_tag="WJK73_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ7557618.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            1747..2187
                     /gene="rimI"
                     /locus_tag="WJK73_001133"
     CDS             1747..2187
                     /gene="rimI"
                     /locus_tag="WJK73_001133"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ7557619.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGLAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            2181..2915
                     /locus_tag="WJK73_001134"
     CDS             2181..2915
                     /locus_tag="WJK73_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ7557620.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            2977..3618
                     /gene="pyrE"
                     /locus_tag="WJK73_001135"
     CDS             2977..3618
                     /gene="pyrE"
                     /locus_tag="WJK73_001135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ7557621.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            3692..4126
                     /locus_tag="WJK73_001136"
     CDS             3692..4126
                     /locus_tag="WJK73_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557622.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            4123..5433
                     /gene="argA"
                     /locus_tag="WJK73_001137"
     CDS             4123..5433
                     /gene="argA"
                     /locus_tag="WJK73_001137"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ7557623.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            5529..7544
                     /locus_tag="WJK73_001138"
     CDS             5529..7544
                     /locus_tag="WJK73_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ7557624.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEARELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(7658..8614)
                     /locus_tag="WJK73_001139"
     CDS             complement(7658..8614)
                     /locus_tag="WJK73_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557625.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            8850..9815
                     /locus_tag="WJK73_001140"
     CDS             8850..9815
                     /locus_tag="WJK73_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7557626.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         2173 a         2993 c         2814 g         1924 t
ORIGIN      
        1 aggcaattga aaattttttg gcgcattttt atgcgtcaaa tttcgttaac agactatttt
       61 tgcaaaggtc tcatattatc tattctgcgt atgactagga gcaaacctgt gaaccgaact
      121 gccttctgct gcctttcttt gaccgccgcc ctgattctga ccgcctgcag cagcggaggc
      181 ggcggtgtcg ccgccgacat cggcgcgggg cttgccgatg cactaaccgc accgctcgac
      241 cataaagaca aaagtttgca gtctttgacg ctggatcagt ccgtcaggaa aaacgagaaa
      301 ctgaagctgg cggcacaagg tgcggaaaaa acttatggaa acggcgacag cctcaatacg
      361 ggcaaattga agaacgacaa ggtcagccgc ttcgacttta tccgtcaaat cgaagtggac
      421 gggcagctca ttaccttgga gagcggagag ttccaagtgt acaaacaaag ccattccgcc
      481 ttaaccgccc ttcagaccga gcaagtacaa gattcggagc attcagggaa gatggttgcg
      541 aaacgccagt tcagaatcgg cgatatagcg ggtgaacata catcttttga caaacttccc
      601 gaaggcggca gggcgacata tcgcgggacg gcattcggtt cagacgatgc cagtggaaaa
      661 ctgacctaca ccatagattt cgccgccaag cagggacacg gcaaaatcga acatttgaaa
      721 tcgccagaac tcaatgttga cctggccgcc tccgatatca agccggataa aaaacgccat
      781 gccgtcatca gcggttccgt cctttacaac caagccgaga aaggcagtta ctctctaggc
      841 atctttggcg ggcaagccca ggaagttgcc ggcagcgcag aagtggaaac cgcaaacggc
      901 atacgccata tcggtcttgc cgccaagcag taaccattgt gaaaatgccg tccgaacacg
      961 ataatttacc gttcggacgg catttttgcg ccataccgcc ctaggatacg acggcacggc
     1021 aggcaagccg gtttcactat ataatgccgt ctgaaccaac gagaaccaca ccatgcaagc
     1081 cgatttcaac cgccccgtcc tcgccgtcga taccggaact tcctatttgt cgctcgcgct
     1141 gcgcgccgac ggcgaaaccc gtctgttcca tcaggaagtc ggcagccgcc agtccgcact
     1201 gatcctgccg gaaatccgca ccctattccg aaatgcaggc attaccgccg ccgatttggg
     1261 ggcgattgtt tatgcgaaag gccccggcgc gtttacggga ctgcgtatcg gtatcggcgt
     1321 ggcgcaaggt ttggcaacgc cgtttgatac gcctttaatc ggcatcccca cgctcgatgc
     1381 cgccgcctcg ctgccgccgc cgcaaagctg catactcgcc gctgcggacg cgcgtatggg
     1441 cgaagtgttt tatgcgtggt tcgacacgct gaaccgccgc cgtttgagcg attatcaggt
     1501 cgggcgggcg gcagacatcc ggctgccgga gggatgtgcc ttttcagacg gcataggcag
     1561 cgcgttcgcg ttggaagaaa cgccgccgtt ttcaggcaaa cccgatatgc cgaccgccgc
     1621 cgacttcctc aatctggcgg ctgaaggcgg ttttcccgcc gtcgctgcgg cacacgccga
     1681 gctgctctac gtccgcaaca aaatcgccct gactgccaaa gaacaggcgg aacggagggc
     1741 gcgcctgtga acatccgccg cgccgtttat gccgattgcg cggcactcgc cgcactcgat
     1801 gccgtctgca acccgtccgc gtggacgcaa cgccaatttg agtctgcatt ggtttcgctg
     1861 tccgaacagg ttttcctcgc ggaaaaagac ggcgggttgg ccgcctttat cgtttggcag
     1921 aacctgcccg acgaatccga actgcacctg attgccaccg cgcccgaatg ccgccgccaa
     1981 ggaattgcgt ccgccctgct cgaatattgg ttcgcacatc tgcccgaagg cacgcaacgc
     2041 ctgctgctcg aagtccgcgc aggcaacacc gccgcgcagg cactgtacgc ggcgcacggc
     2101 ttcagcattg cgggcaggcg gaaaaactat taccgcgcca ccgacgggca aaccgaagat
     2161 gccgtcttaa tggagaaaat atgttaagcg cgcgctacct ccacctgcac gaagctttgg
     2221 gtttgggtcc gatgtggctg aaacaggccg ctgccgtcct gccgcccaaa aacacacccg
     2281 caaccccggc acaggcacgt cctcaaaccg tccgcgccgc cccgatccgc ccttcccaac
     2341 cccataacgg tcaggcgcgg ctcgaaacga tgaaagcgtt ggaaaccgcc gccgtaccta
     2401 cgcgcaaacc cgcgcctgaa accgaaacgc ctctgcccgg cctttcagac ggcatcgccc
     2461 ccgttcccgc cgcttcgggc atcaccaagc ttgccgtcgt cagcctttgc ccaccgatcg
     2521 aggatgcggt ttacgggcaa ctgttccacg gcaaagcggg tgtcctgctt gacaacatac
     2581 tcaaagccgt aggactggat gccgcttatg tacacaagac cagctgggtg aaaaccgccg
     2641 ccgtcggcaa cccgatgccg tctgaacagg ccgtcgcgaa tgcgctgggt caaatcgcca
     2701 gggaactcga cggctgccgt gcccccgccg tccttttcct cgggcaggct tttgtccagc
     2761 ctgaacggca aacgatgatt gaaactttgt gcggcagccg tcccttcttc atcatcgacc
     2821 atcccgctcg gcttttacgc caacccgaac ttaaagcccg aaactggcag gtgttgaaac
     2881 agttgaaaca cgccttgcag gcaggcggca gttgaagcag gccgcacggg gcggtagaat
     2941 cgcaactgcg tcccaatatc tgacagaaag cacaaaatga ccgatttccg ccaagatttc
     3001 ctcaaattct ccctcgccca aaatgttttg aaattcggcg aattcaccac caaagccgga
     3061 cggcggtcgc cctatttctt caatgccggc ctctttaacg acggcttgtc cacgctgcaa
     3121 ctggcaaaat tctatgcaca atccatcatt gaaagcggca tccgattcga tatgctgttc
     3181 ggtcccgcct acaaaggtat tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa
     3241 ggcgtgaacg tcccgtttgc ctacaaccgc aaagaagcca aagaccacgg cgaaggcggc
     3301 gtgttggtcg gcgcgccgct taaagggcgc gtgctgatta tcgacgacgt gatttccgcc
     3361 ggcacatccg tacgcgaatc gatcaaactg attgaagcgg agggtgcaac ccccgccggt
     3421 gtcgccatcg cgctcgaccg catggaaaaa ggcacgggtg aattgagcgc ggttcaggaa
     3481 gtggaaaaac aatacggtct gcccgtcgcc cccatcgcca gcctgaacga tttgtttatt
     3541 ctgttgcaaa acaaccccga attcggacag ttcctcgaac ccgtccgagc ctaccgtcgg
     3601 cagtacggcg tagaataaaa acaaagcata tgccgtccga accgccttac gcctcagacg
     3661 gcatcaaacc tgacacacac gaggaaatac catgcccgcc tgtttctgcc cccactgcaa
     3721 aacccgtctc tgggtcaaag aaacccaact caatgtcgcc caaggcttcg tcgtctgcca
     3781 aaaatgcgaa ggactgttta aagccaaaga ccatctggca agcacgaaag aacccatatt
     3841 caacgatttg cccgaggctg tttcggatgt caaactcgtt caccgcatcg gcacgcgcgc
     3901 cattggcaag aaacagattt cccgcgacga aatcgccggc atcctcaacg gcggtacaac
     3961 cctgcacgat acgccgcccg caaccgccgc cacccctgcc gccgcaccgc aggttgccgt
     4021 accgcccgcc gcgcctgccc gtcaggaagg gttcaactgg acgattgcaa ccctgtttgc
     4081 ccttatcgtc ctcattatgc agctttccta cctcgtcatc ctatgatcgt gcccgacctc
     4141 tttgtcgccc acttccgcga agccgtcccc tacatccgcc aaatgcgcgg caaaacgctg
     4201 gtcgccggca tagacgaccg cctgctcgaa ggtgatacct taaacaagct cgccgccgac
     4261 atcgggctgt tgtcgcaact gggcatcagg ctcgtcctca tccacggcgc gcgccacttc
     4321 ctcgaccgcc acgccgccgc gcaaggccgc acgccgcatt attgccgggg cttgcgcgtt
     4381 accgacgaaa cctcgctcga acaggcgcag cagtttgccg gcaccgtccg cagccgtttt
     4441 gaagccgcat tgtgcggcag cgtttccggg ttcgcgcgcg cgccttccgt cccgctcgta
     4501 tcgggcaact tcctgaccgc ccgtccgata ggtgtgattg acggaaccga tatggaatac
     4561 gcgggcgtta tccgcaaaac cgacaccgcc gccctccgtt tccaactcga cgcgggcaat
     4621 atcgtctggc tgccgccgct cggacattcc tacagcggca agaccttcta tctcgatatg
     4681 cttcaaaccg ccgcctccgt cgccgtctcg cttcaggccg aaaaactcgt ttacctgacc
     4741 ctttcagacg gcatttcccg ccccgacggc acgctcgccg aaaccctctc ggcacaggaa
     4801 gcgcaatcgc tggcggaaca cgccggcggc gaaacgcgac ggctgatttc gtccaccgtt
     4861 gccgcgctcg aaggcggcgt gcatcgcgtc caaatcctca acggtgcctc cgacggcagc
     4921 ctgctgcaag aactcttcac ccgcaacggc atcggcacat ccattgccaa agaagccttc
     4981 gtctccatcc ggcaggcgca cagcggcgac atcccgcaca tcgccgccct catccgcccg
     5041 ctggaagaac agggcatcct gctgcaccgc agccgcgaat acctcgaaaa ccacatttcc
     5101 gaattttcca tcctcgaaca cgacggcaac ctgtacggtt gcgccgccct gaaaaccttt
     5161 gccgaagccg attgcggcga aatctcctgc cttgccgtct cgccgcaggc acaggacggc
     5221 ggctacggcg aacgcctgct tgcccacatt atcgataagg cgcgcagtat gggcataagc
     5281 aggctgttcg ccctctccac aaataccggc gaatggtttg ccgaacgcgg ctttcagacg
     5341 gcatcggaag acgagttgcc cgaaacgcgg cgcaaagact accgcagcaa cggacgaaac
     5401 tcgcatattc tggtacgtcg cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg
     5461 ccgtctgaac cccgtttcag acggcatttc cccgattata aagacctgcc caaccctcaa
     5521 ggaccccgat gaaatcctac cccgacccct accgccattt tgaaaacctc gattccgccg
     5581 aaacgcaaaa cttcgctgct gaagcgaatg ccgaaacgcg cgcgcgtttt ttaaacaacg
     5641 acaaggcgcg cgcgctttca gacggcattt tgaatcaaat gcaggacacg cggcagattc
     5701 cgttttgtca ggaacaccgc gcgcggatgt accatttcca tcaggacgcg gaatatccga
     5761 agggcgtgta ccgcgtgtgc acggcggcga cctaccgttc gggctatccg gagtggaaaa
     5821 tcctgttttc agtggcggac ttcgacgaat tgctcggcga cgatgtatat ttgggcggcg
     5881 tgtcgcactt ggtggaaaag cccaaccgcg cgttgttgac actaagcaaa tcaggcggcg
     5941 atacggcgta cacgctggaa gtggatttgg aagcaaggga attggtagaa ggtggttttc
     6001 actttccggc aggcaaaaac caagtgtcgt ggcgcgatga aaacagcgtg tgggtgtgtc
     6061 cggcttggga cgaacgccag ttgaccgaat cgggctatcc gcgtgaggtg tggctggtgg
     6121 aacgcggcaa gagtttcgag gaaagcctgc ctgtgtatca aatcgataaa gatgcaatga
     6181 tggtgaacgc gtggcgttat ctcgatccgc agggttcgcc gattgatttg attgaagcgt
     6241 cggacggttt ttacaccaaa acctatttgc aggtgtcggc cgaaggcgag gcgaaaccgt
     6301 taaacctgcc caacgattgc gatgtggtcg gctatctggc ggggcatctt ttgctgacgc
     6361 tgcgcaagga ctggaaccgc gcgaaccaaa gctatccgag cggtgcgctg gtggcggtga
     6421 agctgaatcg gggtgaactc ggggcggcgc agcttttgtt tgcgcctgat gaaacgcagg
     6481 cattggaaag cgtggaaacg accaagcgtt ttgtggtggc gagcctgctg gaaaacgtac
     6541 aaggccgtct gaaagcgtgg cgttttgccg acggcaaatg gcaggaagtc gaactgccgc
     6601 gcctgccttc gggcgcgttg gaaatgaccg accagccttg gggcggcgac gtggtttacc
     6661 ttgccgccgg cgatttcacc acaccgctga cgctgtttgc gctggatttg aacgtgatgg
     6721 aactgaccgt catgcgccgc cagccgcagc agttcgattc agacggcatc gaagtgcggc
     6781 agttttgggt gatgtcgtcc gacggcgaac gcatccctta tttccacgtc ggcaaaaacg
     6841 ccgcgcccga cacgccgacg ctggtttatg cctacggcgg tttcggcgta cccgagctgc
     6901 cgcattatct gggcagcatc ggcaaatact ggctggaaga gggcaacgcc ttcgtgttgg
     6961 cgaacatacg cggcggcggc gagttcggcc cgcgctggca tcaggcggcg cagggaatca
     7021 gcaaacacaa aagcgtcgat gatttgcttg ccgtcgtgcg cgatttgtcc gagcgcggca
     7081 tcagttcgcc ccaacacatc ggtttgcagg gcggcagcaa cggcggcctg attaccgccg
     7141 ccgccttcgt gcgcgaaccg caaagcatag gcgcactggt gtgcgaagtg ccgctgaccg
     7201 acatgatccg ttatccgctg ctctccgccg gttcaagctg gacagacgaa tacggcaatc
     7261 cgcaaaaata cgaagtctgc aaacgccggt tgggcgaatt gtcgccgtat cacaatcttt
     7321 cagacggcat cgattatccg cccgcgctca tcaccaccag cctcagcgac gaccgcgtcc
     7381 atcccgccca cgcgctcaag ttttacgcca aactgcgcga aacctcgccg caatcttggc
     7441 tctactcgcc tgacggcggc ggccataccg gcaacggcac gcagcgcgaa gccgccgacg
     7501 aactcgcctg cgtgttgctg tttttgaaag agtttttggg ctaagggcgg ggagcggcac
     7561 tgccgccgcg aatgaaaaaa ggtcgtctga aactgctttt tcagacgacc ttttttaatg
     7621 gttgtttcaa atcaaaatat ctatgccgcc ggccccatca gcacttcttc acatccgaag
     7681 gcaaaaatcc gtaatgccgt ctgaacgctt cgttgaaccg tcccgcgtgg cggtagccgc
     7741 aaaagtgcat ggcggcttgg acggtgctgc cggattcgat gagggcgagc gcgtgttcca
     7801 gccgcaggcg gcgcaggcat ccggcgacgg tttcgccggt ttgcgctttg aaatagcgtt
     7861 tcaggtagca ttcgttcagt ccgacgcggc gggcgatttc ggcgatggtc agcggacggg
     7921 cgaattcgtg ttgcaggatg tcggcggctt cgtctatgcg ccgacggcgg taaccgttgt
     7981 cgtggcggcg gaaggtgaag cgcaataatc gggcggagag ttccagcgcg gcggcttcgt
     8041 cggcaagcag gccgaagccg tccgattcga acgagcgttg cagcagtggg caggccgccg
     8101 ccgtcagtgc cgctgcgttt tgcgccagcc gttgcagggc gaatcggcct atcgtttgcg
     8161 gcgaaaacag gcgttcgtcc agcaagcctt cgtcgtgcca gcggcgcagt ttttccagcg
     8221 aaaaatccaa atgcagcgcg cacatgccgc tgttgtcggg cagcagggtt tcggatacgt
     8281 ccgccaactc gccgcgtacc agccagattt cgccggcaga cgggcggtat tccctgccgc
     8341 ccatttgtaa ccggttctgc cccgacacca tgacgaacaa ggcgcagttg tggctgaaat
     8401 tgtggatttc ggtgggaaac gcgcccgttc cgccgccgcg catccgcgac aaggtgatgc
     8461 ccgagtcgaa gcggttgatg cacatttcca gatgcaaacc gggctgtttt gcctgcgcaa
     8521 tgagcgcgct gtcggaacag ccgtccaacg cccagccgga tttatcggag cggacatagg
     8581 tttggtactg gcggtagatg gcggcggtgt tcatgattgg ataggaacga gttgtctaac
     8641 aaatgaatta aataggaatt attaccaata atcaagcgca gggattggtt gaaacggaaa
     8701 aggtcgtctg aaagggtgtt tcagacgacc ttttccgtat cgggaatttg ttttgccgta
     8761 tcgggaattt tgcgttttgc ggcgtggttt ctgcaggttg tttgcttaat aataaacatt
     8821 cttattcgta tgcaaaggaa ccgcacaccg tgaaaccgcg tttttattgg gcagcctgcg
     8881 ccgtcctgct gaccgcctgt tcgcccgaac ctgccgccga aaaaactgta tccgccgcat
     8941 ccgcatctgc cgccacactg accgtgccga ccgcgcgggg cgatgccgtt gtgccgaaga
     9001 atcccgaacg cgtcgccgtg tacgactggg cggcgttgga tacgctgacc gaattgggcg
     9061 tgaatgtggg cgcaaccacc gcgccggtgc gcgtggatta tttgcagcct gcatttgaca
     9121 aggcggcaac ggtggggacg ctgttcgagc ccgattacga agccctgcac cgctacaatc
     9181 ctcagcttgt cattaccggc gggccgggcg cggaagcata tgaacagttg gcgaaaaacg
     9241 cgaccaccat agatctgacg gtggacaacg gcaatatccg caccagcggc gaaaagcaga
     9301 tggagacctt ggcgcggatt ttcggcaagg aagcgcgcgc ggcggaattg aaggcgcaga
     9361 ttgacgcgct gttcgcccaa acgcgcgaag ccgccaaagg caaaggacgc gggctggtgc
     9421 tgtcggttac gggcaacaag gtgtccgcct tcggcacgca gtcgcggttg gcaagttgga
     9481 tacacggcga catcggccta ccgcctgtag acgaatcttt acgcaacgag gggcacgggc
     9541 agcctgtttc cttcgaatac atcaaagaga aaaaccccga ttggattttc atcatcgacc
     9601 gtaccgccgc catcgggcag gaagggccgg cggctgtcga agtattggat aacgcgctgg
     9661 tacgcggcac gaacgcttgg aagcgcaagc aaatcatcgt catgcctgcc gcgaactaca
     9721 ttgtcgcggg cggctcgcgg cagttgattc aggcggcgga gcagttgaag gaggcgtttg
     9781 aaaaggcaga acccgttgcg gcggggaaag agtagggagt cgtctgaaaa cggagtttcc
     9841 gaaggaagcg gggggtttct gcgaagctaa agtgcggttt caacgaattg aaaagcagcc
     9901 tgta
//
LOCUS       DAWKOD010000061         9847 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000061 DAWKOD010000000
VERSION     DAWKOD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.061"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJK73_001141"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJK73_001141"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ7557627.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLKYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1445..1831)
                     /locus_tag="WJK73_001142"
     CDS             complement(1445..1831)
                     /locus_tag="WJK73_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7557628.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYDQTKLKQNKYFKITLSKSPDHFTLKATPNPATNDG
                     ETCVVTLNDGGIIAASGTNQSCPGFD"
     gene            2057..3235
                     /locus_tag="WJK73_001143"
     CDS             2057..3235
                     /locus_tag="WJK73_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ7557629.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3301..5235
                     /locus_tag="WJK73_001144"
     CDS             3301..5235
                     /locus_tag="WJK73_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ7557630.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            5249..5471
                     /locus_tag="WJK73_001145"
                     /pseudo
     CDS             5249..5471
                     /locus_tag="WJK73_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5554..6336)
                     /locus_tag="WJK73_001146"
     CDS             complement(5554..6336)
                     /locus_tag="WJK73_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ7557631.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAKGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6475..8664
                     /locus_tag="WJK73_001147"
     CDS             6475..8664
                     /locus_tag="WJK73_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ7557632.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVEIL
                     NAGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHRESIPFKCPDCGNQDLTAVGHGTQRVEETLRSFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8710..9784)
                     /locus_tag="WJK73_001148"
                     /pseudo
     CDS             complement(8710..9784)
                     /locus_tag="WJK73_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
BASE COUNT         2300 a         2544 c         2868 g         2135 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatga ccgcagccgc attctttttt catatcgatc accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggaacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcctcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gcgcgccgaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacgc cggaaacttt aatggcttta taagtggtca
      721 cgccggcaca agtaatgctc gaagcttgcg caggatccaa accttcaggg actttgaccg
      781 cgtaatcggc actcacgatg cagtgggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacgga acggcacagg gtttcgcggc cggtattgca gtattcgcac gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtatttcaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catcttacag
     1261 gtcaagtggt atgttgttga aaaatatatg ttatattata acagctcgga aaggcgcggc
     1321 atcggggcgg ttgccggggc ggcaggtttc aagtttgaaa aaccggatgg gcaaacccgt
     1381 aaagataccg tctgaagctg tgtccggacg gcatctttac gggtttgcgg gcttcggcgg
     1441 aggattagtc gaagccgggg caggattggt ttgtaccgga agcggcaatg ataccgccgt
     1501 cgttgagtgt aacgacgcag gtttcaccgt cgttggtggc gggattgggc gttgccttaa
     1561 gggtgaagtg gtcggggctt ttgcttaagg taatcttgaa atatttgttt tgtttcagtt
     1621 tggtttggtc gtagttgtca aacgtccctt tttggcggta gtaacgctcc aagttttgcg
     1681 cgttcatcag cagggtggat ttgacttccg acaggcggac gcgccggatg taggttttat
     1741 aggaagggta ggtgatgagc gtcaggatgc cgaggatggc gacggcaatc atcagctcga
     1801 gcagcgtaaa gcctttttga acgtttttca tagcaatgtg tttccatttg tttgtcggtc
     1861 ggcgacatta taacgccgta tcggaaatgg cggaatatgt aaacggattg aaattttcgg
     1921 gaaagcagat tgtataagcc atttaaaaca aatggttatt tttattgtcg gcagtttgcc
     1981 gccttggatg gggcagggac ttgcggtaga atccgcttcc gatttatggg attgacgcat
     2041 acagagaatt gaaaacatgg caaaaatgat gaaatgggcg gctgttgcgg cggtcgcggc
     2101 ggcagcggtt tggggcggat ggtcttatct gaagcccgag ccgcaggctg cttatattac
     2161 ggaaacggtc aggcgcggcg acatcagccg gacggtttct gcaacagggg agatttcgcc
     2221 gtccaacctg gtatcggtcg gcgcgcaggc atcggggcag attaagaaac tttatgtcaa
     2281 actcgggcaa caggttaaaa agggcgattt gattgcggaa atcaattcga cctcgcagac
     2341 caatacgctc aatacggaaa aatccaaatt ggaaacgtat caggcgaagc tggtgtcggc
     2401 acagattgca ttgggcagcg cggagaagaa atataagcgt caggcggcgt tatggaagga
     2461 aaacgcgact tccaaagagg atttggaaag cgcgcaggat gcgtttgccg ccgccaaagc
     2521 caatgttgcc gagctgaagg ctttaatcag acagagcaaa atttccatca ataccgccga
     2581 gtcggaattg ggctacacgc gcattaccgc aacgatggac ggcacggtgg tggcgattct
     2641 cgtggaagag gggcagactg tgaacgcggc gcagtctacg ccgacgattg tccaattggc
     2701 gaatctggat atgatgttga acaaaatgca gattgccgag ggcgatatta ccaaggtgaa
     2761 ggcggggcag gatatttcgt ttacgatttt gtccgaaccg gatacgccga ttaaggcgaa
     2821 gctcgacagc gtcgaccccg ggctgaccac gatgtcgtcg ggcggttaca acagcagtac
     2881 ggatacggct tccaatgcgg tctactatta tgcccgttcg tttgtgccga atccggacgg
     2941 caaactcgcc acggggatga cgacgcagaa tacggttgaa atcgacggcg tgaaaaatgt
     3001 gctgattatt ccgtcgctga ccgtgaaaaa tcgcggcggc aaggcgtttg tgcgcgtgtt
     3061 gggtgcggac ggcaaggcgg cggaacgcga aatccggacc ggtatgagag acagtatgaa
     3121 taccgaagta aaaagcgggt tgaaagaggg ggacaaagtg gtcatctccg aaataaccgc
     3181 cgccgagcaa caggaaagcg gcgaacgcgc cctaggcggc ccgccgcgcc gataaacgaa
     3241 tatgccgtct gaacacggaa acggtttcag acggcatttg ttattgattt acggaatatt
     3301 atgagcttga tcgaatgtaa aaacatcaac cgctatttcg gcagcggcga gaaccgcgtc
     3361 catattttga aagacatcag cctgtcgata gagaagggcg attttgtcgc catcatcggg
     3421 cagtccggtt cgggaaagtc cacgctgatg aacatcctcg gctgtttgga taccgccggt
     3481 tccggttcgt accgaatcga cggcatcgaa actgccaaaa tgcagcctga cgagctggcg
     3541 gcattgcgcc gcgaacgctt cggtttcatc ttccaacgct acaacctctt aagctcgctg
     3601 accgcaaggg ataacgtcgc gctgccagcc gtctatatgg gcgcgggcgg caaagagcgt
     3661 tccgcgcggg cggagaagtt gttacaggat ttgggtttgg caagcaaaga gggcaacaag
     3721 cccggcgaac tctcgggcgg acagcagcag cgcgtctcca tcgcccgcgc cctgatgaac
     3781 ggcggagaaa tcatcttcgc cgacgagccg accggcgcac tcgataccgc cagcggcaaa
     3841 aacgtgatgg aaatcatccg caggctgcac gaagccgggc ataccgtcat tatggtcacg
     3901 cacgaccccg gcatcgccgc caatgccaac cgcgtcatcg aaatccggga cggcgaaatc
     3961 atttccgaca cctcgaaaaa tcccgaaatc cccgcaagca atgtcgggag gattcgggaa
     4021 aaagcctcgt ggtcgtttta ttacgaccag tttgtcgaag ccttcagaat gtcggtgcaa
     4081 gcagtattgg cgcacaaaat gcgttcgctt ctgacgatgc tcggcatcat catcggtatc
     4141 gcttcggttg tctccgtcgt cgcattgggc aatggttcgc agaaaaaaat ccttgaagac
     4201 atcagttcga tagggacgaa caccatcagc atcttcccgg ggcgcggctt cggcgacagg
     4261 cgcagcggca ggattaaaac cctgaccata gacgacgcaa aaatcatcgc caaacaaagc
     4321 tacgttgctt ccgccacgcc catgacttcg agcggcggca cgctgaccta ccgcaatacc
     4381 gacctgaccg cttctttgta cggcgtgggc gaacaatatt tcgacgtgcg cggactgaag
     4441 ctggaaacgg ggcggctgtt tgacgaaaac gatgtgaaag aagacgcgca ggtcgtcgtc
     4501 atcgaccaaa atgtcaaaga caaactcttt gcggactcgg atccgttggg taaaaccatt
     4561 ttgttcagga aacgcccctt gaccgtcatc ggcgtgatga aaaaagacga aaacgctttc
     4621 ggcaattccg acgtgctgat gctttggtcg ccctatacga cggtgatgca ccaaatcaca
     4681 ggcgagagcc acaccaactc catcaccgtc aaaatcaaag acaatgccaa tacccaggtt
     4741 gccgaaaaag ggctgaccga tctgctcaaa gcgcggcacg gcacggaaga tttcttcatg
     4801 aacaacagcg acagcatcag gcagatagtc gaaagcacca ccggtacgat gaagctgctg
     4861 atttcctcca tcgccctgat ttcattggta gtcggcggca tcggcgtgat gaacatcatg
     4921 ctggtgtccg ttaccgagcg caccaaagaa atcggcatac ggatggcaat cggcgcgcgg
     4981 cgcggcaata ttttgcagca gtttttgatt gaggcggtgt taatctgcgt catcggcggt
     5041 ttggtcggcg tgggtttgtc cgccgccgtc agcctcgtgt tcaatcattt tgtaaccgac
     5101 ttcccgatgg acatttcggc ggcatccgtt atcggggcgg tcgcctgttc gaccggaatc
     5161 ggcatcgcgt tcggctttat gcctgccaat aaagcagcca aactcaatcc gatagacgca
     5221 ttggcgcagg attgaggttg gaacaaagat gccgtctgaa gctgcaggac cggtcatttt
     5281 ggagcagaaa cttattggac aaaaacggtt tcttagattc tacgttccag attcccactt
     5341 gcgtgggaat gacggcggtg tgtttcgtat tgttcagttt attattttca atcattcaat
     5401 gggttaggat gtgtttgttg gcttgctgta ctggtttaaa tttaatccac tataaattta
     5461 atccactata actttcagtg cggattggtt ttcagacggc atttcccgca aaaaaggctt
     5521 ggaattttcc aagccttttg ttttgcggat ggattattga tttttgcgga tgatttcctc
     5581 cagttggggc atcgggctgt agccgctttg gctgcgcccg ttggggaaga cgagggtcgg
     5641 cgtgccgttg aagccgaatt gttcgcccaa ggaagtggtt tccgcgacgg gattgtcgca
     5701 gatgctgccg ccgaccggga atttgccttt acgcatccaa tccgtccacg ctttggcgcg
     5761 gtcgggctga caccataaga tttgcgcctt gcgcgcggca tcggggtgca ggccggcaat
     5821 gggcatcata aagctgtaaa ccgtcacgtc ggtcattttt tcaaactcgt gttccaagcg
     5881 tttgcagaac ggacaatcgg ggtcggagaa gacggcgact ttcagcttgc cgttgccgcg
     5941 cacttctttg atggctttgt ccaaaggcag ggaggcgaag tcgattttgt tcaaatcggc
     6001 ggcgcgttct tcggtcaggt ttttgcgcgt gtcgatgttg atgagttcgc cgacgaacat
     6061 atagccgcct ttggcatcgg tgtagataat ctgcctgccg ctgacgacga cttcgtaaat
     6121 gcctttgacc ggtgtttcgc tgacgctcaa cactttcaaa tcttgggcgg aataggtttt
     6181 ttccaaacgc gctttcaaag aggcggcaac ggatttgccg gcggactcgg ctttgacggc
     6241 gggttcggcg ttggcattgg aaacgggcgt ttgcccgcaa gccagcagtg ggaggacggt
     6301 aaagggggtc aagattttga ttaacttggt tttcatataa agatgattgc gcgtgttgga
     6361 aaagcggaat tgtatcaaat ctctgttgcg cctgcattgc gcctaggctc aatttatcgt
     6421 ctgaaaatag cttccgcctg ttaaaatacg caaaaaaatg atttgcctgc ctgtatgatc
     6481 taccaccgca tcgccgtaaa cgtgccgctt tcagacggcc ttttgactta ttcccattcc
     6541 gatccgcttc ctccgggaac gcgggtgctt gtgcctttcc gcaataaaac cgtggtcggg
     6601 atggtgtggg aaacggatat tgcgccggat atggatacgg cgcggatttt gggcgttcag
     6661 acgacctttt cggacgaacc gctgctgccc gaaagctggc gtgatttgtt ggcgtttacg
     6721 tcgcgttatt accactatcc gaccgggcag gcagtatttg ccgcattgcc gcaaggtttg
     6781 aaggaaacgc gcgcggtgga aatgccgcag ccgcccttgt tttacgcgtt gaacgaagcg
     6841 ggcagggcgc aaacgccgcc gccggcgcgg ttcaacaaaa aagcggcttt gtgggacgcg
     6901 ctgctgtcgg gcgaaatgac gatggcagcg ttgaagcagg taaacgcgca ggcggcgaag
     6961 ttaatcgaag attgggcgga gcagggttgg attgaaacga cggaagcggc gaagcccgtt
     7021 ttaaggtcgt gtaatgggca ggcttcgcac tctgaatttg tattaaatgc tgaccagcag
     7081 aaggcttccg atgccattca gacggcattg ggacgcttcc agccgtttct gctgtacggc
     7141 atcaccggca gcggcaagac cgaagtgtat ttcgatgcga tggcgaaagt gttggctcag
     7201 gggcggcagg tgttgtttct gttgcccgaa atcaacctca cgccgcagct tttgaagcgg
     7261 gtggaaaacc gttttgccga cgtgccgacc gccgtgttgc acagccggat ggcggcaggc
     7321 aggcgaacgc aggattatct gcgcgcgatg tcggggcagg cgaagctggt gatcggcacg
     7381 cggctggcgg tgttcacgcc gatggatgat gtcgggctga ttgtggtcga tgaggaacac
     7441 gacggctcgt tcaaacagga caacgaattg cgctaccacg cccgcgattt ggcggtgtgg
     7501 cgggcgaagc agggcggctg tcccgtcgtg ttgggcagcg cgaccccaag cttggaaagc
     7561 tggcacaagg cacaaagcgg cgcgtaccgc ctcttgcaac tgaccgaacg cgcccatgcc
     7621 gccgcgcaac tgccgcaagt agaaatcctc aacgcaggcc gtctgaaact cgacaacggt
     7681 ttctcgccgc aagccttgca gcttctgaaa cagaattttg aagcaggcgg tatgtcgctg
     7741 gtgtacctca accggcgcgg ctttgcgccc gcgctgtttt gcggcgactg cggccatacc
     7801 ttcggctgcc cgaactgctc cgccaaaatg gtgttgcacc aacgcgcccg ccaactgcgc
     7861 tgccaccact gcgaccaccg cgaatccatc ccgttcaaat gccccgactg cggcaaccaa
     7921 gacctgaccg ccgtcggcca cggcacgcag cgcgtcgaag aaaccctgcg ctccttcctg
     7981 cccaaggccg ccgtcgtccg tgttgacagg gacagcacgg cgcacaaaaa cgactgggcg
     8041 gatttgtacc gccgcattgc cgacaacgaa atcgacattc tggtcggcac gcagatgctc
     8101 gccaaaggcc atgatttcgc gcggctcaac ctcgttatcg tgttgaacgc cgacggcagc
     8161 ctgtacagcg cggacttccg cgcaccggaa aggttgtttg ccgagttgat gcaagtgtcc
     8221 ggcagggcgg ggcgcgccga caaacccggc aaggtgctga tacagaccca actgcccgaa
     8281 catcccgtct tcgccgccgt caaagcgcag gattatgccg tgtttgccga aaatgaattg
     8341 aacgagcggc aaatgttcgc gatgccgccc ttcggtttcc agaccgccgt ccgcgccgat
     8401 gcgccgcgtg ttgccgatgc gatggaattt ctcaatgccg ccaaagaaac cctcgccccg
     8461 ctgttgcccg aaagcgtttc ccagtttggc gccgccccta tgctgatggt gcgccttgct
     8521 gaacgcgaac gcgcgcaaat cttcctcgaa tcggtatccc gacaggattt gcaccgcgcc
     8581 gtgagtttgt gggtgcaggt gttgcagcaa aaccgcgaca gcaaaatcag atggtcggtg
     8641 gatgtcgatc cgcaggaggc ttgattatcg gcaatacgat gccgtctgaa accgtttcag
     8701 acggcatttt tattccgggt cgtccgtaaa cgcattcgcc cgaaaaatcg gaacaaacgt
     8761 gaaaagataa agcgtgaaca cgacggcggt cagaatcgcg gggacggtga tgaagaatat
     8821 tggattcacg ttcatcaaga aagcacgcga gacggcagcc ataaagagca ggaggacggc
     8881 gatgaggcag agtttcgggt agtcgagctt ggtaaagccg ctgtgccaca gtccggcggt
     8941 cagccacacc atcatcacgc cgcccatcat gtcgccgagg gtaatcaggt gcaggggcgc
     9001 ggaggcgggc aggttttgca gtttcgccgc acctgtccac aaatagcctg cggcggcaaa
     9061 aagctggagc aggtaataag tgcggacgta gtgtttgcgc aggagttcgt gatggtgcag
     9121 ttcgcgcagc ttggcgagca ggatgaaacc gacggcaagc gcggtaaaac cggcggtttg
     9181 cgcaggcagc caaagttcgg cggcggcgtg cagcagcagg aaggtgatgg cgatgttttt
     9241 atagacaacg ttggggatga atacggggtc tttcagacgg cattctttca gggcttccgc
     9301 gcccaaaaga atactgacgc gcacggatac gaacatcacc gccgccatat ttagatgcac
     9361 ttgcgcgcgc aacaggttca aatcgccgct gacggcatat gccgtctgaa aaacagtgaa
     9421 cgcggcaagt aacattagca gggcgaagtt gtcggtgttt cggtctagcc aaatcagccg
     9481 ggcgcagaac agcagcaaca ccagccaata ggcggcgacg aaaaacgagg cagtttgcgg
     9541 cgaaaagggc agtatagcgg atgcggcgag caataatgcc gccatcaaag tcgcgacagg
     9601 tttcaggtta cccgaaaaaa accgtccagt ccaacaaagc cgcagtcagg aaaccaccat
     9661 atgccgcagg tagcatgagt tctaagaaga tttggcggtg caggacgacg gcaccggggc
     9721 tgatgaaaaa caccaacgcg ccgagtatgg caagcactgt cgcgccgacg aaaaacgggc
     9781 gcatagcaac tgtatttttc accccgtcgg gcaaaaatac caaaactcaa atcaagccgt
     9841 ttagata
//
LOCUS       DAWKOD010000062         9794 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000062 DAWKOD010000000
VERSION     DAWKOD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.062"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..1305)
                     /locus_tag="WJK73_001149"
     CDS             complement(10..1305)
                     /locus_tag="WJK73_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7557633.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDHEFIDDPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WJK73_001150"
     CDS             complement(1431..2318)
                     /locus_tag="WJK73_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7557634.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGMLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WJK73_001151"
     CDS             complement(2318..3283)
                     /locus_tag="WJK73_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7557635.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WJK73_001152"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WJK73_001152"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557636.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WJK73_001153"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WJK73_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WJK73_001154"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WJK73_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ7557637.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WJK73_001155"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WJK73_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ7557638.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WJK73_001156"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WJK73_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ7557639.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WJK73_001157"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WJK73_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ7557640.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8694..>9794
                     /locus_tag="WJK73_001158"
     CDS             8694..>9794
                     /locus_tag="WJK73_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ7557641.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRASIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2516 a         2583 c         2424 g         2271 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgcata aaaaacattc gcggccaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 gtcaagctgc ggaaaaactt taagcatatc ttggcggaca agttcggtca tacgcgcggg
      361 atcgtcgata aattcatggt ctttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctaacggcaa tcgccttgcg
      541 ctccaaaggc ttgaatatcg gattcaaacc ggcataagta ttgacggcgt acaccagatt
      601 tttgcattcg acgctgcctt cgggggtgta aaccagccaa ccgttttgat aaggttcaat
      661 gcgtatcatg ggagaatgct caaaaatctt cgtaccagct tcggcagcgg cgcgggcgat
      721 gcccaacgtg taattgagcg gatggagatg cccggacaag ggatcgaact gtgcgccttg
      781 gtacatatcg ctgtcaagct gctgcttcag ttcagtgtta tcccagagtt gataatgagt
      841 tgcaccgtaa tatttttggg cgtgctcatg ccattgttgc aattcttccc aatgctgcga
      901 acggatggca accgtggcat aaccgcgctg ccaatcgcaa tcaatggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcttgctc
     1021 caaaccgacc tgtttttcaa tttcctccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 gggtatattt tcggacggaa tgatacaggc tgtcgagcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgatcagg
     1561 atggttgcca aaacgttcat ctgcggatca agacccagct tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgat
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtatgccat gcacagcgtc
     1861 gtatgtccta agaaaatagt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc ataccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcccgcc agcatgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca aaatggtgtc gttttccaac
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatgc cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggcg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc gcacgctgcc
     3241 ccggacggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 tttgcagata aggcttggtt gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatgtt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct tttgggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacggca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaata cggcggcaat aaaggtaacc aacgaaataa aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttacctgcc gcagtttcgg cttcaggttt ccataccgca ccgatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggaggaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttggaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggcgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc ctgtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccgaaa atgctgccgc ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gattagcaga gtttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtctgcac ctgactgctg gatgacgggt tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagtgcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcaa gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gcttttccga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcagaaat cgcattgcgt tccacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tattgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccagt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt tcccgcctgg
     9001 ataacgacac tgcaatcagc acaggctcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt ttcctttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccgggaac gggcagccgc acattccgcg
     9421 aagccgtccg cagacagtgg ctgccccggc ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcgtatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAWKOD010000063         9766 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000063 DAWKOD010000000
VERSION     DAWKOD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.063"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(312..545)
                     /locus_tag="WJK73_001159"
     CDS             complement(312..545)
                     /locus_tag="WJK73_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ7557642.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WJK73_001160"
     CDS             complement(711..911)
                     /locus_tag="WJK73_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ7557643.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJK73_001161"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJK73_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ7557644.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WJK73_001162"
     CDS             complement(2010..2450)
                     /locus_tag="WJK73_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ7557645.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WJK73_001163"
     CDS             2771..3709
                     /locus_tag="WJK73_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ7557646.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3837..4130
                     /gene="hfq"
                     /locus_tag="WJK73_001164"
     CDS             3837..4130
                     /gene="hfq"
                     /locus_tag="WJK73_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ7557647.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            4215..4781
                     /locus_tag="WJK73_001165"
     CDS             4215..4781
                     /locus_tag="WJK73_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7557648.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4835..5209
                     /locus_tag="WJK73_001166"
     CDS             4835..5209
                     /locus_tag="WJK73_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ7557649.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5219..5713
                     /gene="folK"
                     /locus_tag="WJK73_001167"
     CDS             5219..5713
                     /gene="folK"
                     /locus_tag="WJK73_001167"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ7557650.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(5841..6320)
                     /locus_tag="WJK73_001168"
     CDS             complement(5841..6320)
                     /locus_tag="WJK73_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ7557651.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJK73_001169"
     CDS             complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJK73_001169"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ7557652.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7320..7688)
                     /locus_tag="WJK73_001170"
     CDS             complement(7320..7688)
                     /locus_tag="WJK73_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ7557653.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7808..9427
                     /gene="mnmC"
                     /locus_tag="WJK73_001171"
     CDS             7808..9427
                     /gene="mnmC"
                     /locus_tag="WJK73_001171"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ7557654.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEYILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         2614 a         2989 c         1959 g         2204 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acattgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccac agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgcg aatataaata aaaacggttc gcattgcaag gtcggtatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc aaagtagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcaccctt tcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 aaagccttgt cccgcaaacc gtgcggcgtg tcggtatccg gggcggcaag cagtcgggaa
     1621 atctgctgct cggtgatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccagcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacgacacgc agacgcaccg ccgcaatttc caaaccaacc
     2521 ccgattctac cgccccaaag gacagggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttccaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaac caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgct cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa ttggtaattt ttcctattca aggctacaat
     3781 tcgacacgca cagggcgtct gcccgatttc acaatcataa taacggagtt aaaaatatga
     3841 cagctaaagg acaaatgttg caagatccct tcctgaacgc cctgcgtaaa gagcacgttc
     3901 cggtttcgat ttacctggtc aacggcatca aactgcaagg tcaggttgaa tctttcgacc
     3961 aatacgtcgt tcttctgaga aacacttccg taacccaaat ggtttacaaa cacgccattt
     4021 ccaccatcgt accggctcgc tccgtcaacc tccaacacga aaacagaccc caagccgcac
     4081 cggcttcgac ccttgtccaa gtggaaaccg tccagcagcc tgccgaataa tacgtacgaa
     4141 gcatgacgtg tcatatcttt caatacctta ccggacaacg gtaaggtatt tttattttca
     4201 gacggcattt gaaaatgtta ttgaaaaaca tccttccatt cgcccattgc cttttgcggc
     4261 aggcacttcc cgaaggcggc aatgcgctgg acggcaccgc cggcaacgga cacgacaccc
     4321 ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac atccagccgc
     4381 aagccctgag ccgcactcga tgccgtctga aagaagcgag atacgagaat gtgcaactca
     4441 tcttagacgg acacgaaaac ctgaaacaat atgtttcaga accactaaat gccgccattt
     4501 tcaactttgg ctggctgccc ggcggagata aaagccttac cacccatacg gaaaccagca
     4561 ttcccgccct ttgcgcaacc ctctccctgc tgaaagaagg cggtatgctt attgccgtcc
     4621 tctatccggg acacgaaaac ggcaaacagg aggcagaagc aatcgaacaa tgggcaaaaa
     4681 acctgcctca agaacagttt gccgttttgc gttacggctt taccaaccgg aaaaacagcc
     4741 cgccctatct tttggcattt gaaaaactgc gtcaaaaata actgtttgcg gtaaaataag
     4801 ccgattctaa cccttatttt gatacgagcc gaccatgcag tatctgattg tcaaatacag
     4861 ccatcaaatc ttcgttacca tcaccatttt ggtattcaac atccgttttt tcctactttg
     4921 gaaaaatcca gaaaagccct tggcgggctt ttggaaagca ctgccccacc ttaacgacac
     4981 catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc cgttcaacgc
     5041 gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg gtatgatgat
     5101 gatgcgcgcc cgtccgcgtt caaccaagtt ctacaccgtt tacctgctcg ctatgtgttg
     5161 cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg ccgttctgaa acaccgttat
     5221 gaacaacaga cattttgccg tcatcgccct aggcagtaat cttgaaaacc ctgcccaaca
     5281 ggtacgcgcc gcattggaca cgctgtcgtc ccatcccgat attcggcttc atcaaacctc
     5341 gtccctgtat gccaccgccc ctgtcggcta tgacgaccag cccgactttg tcaatgccgt
     5401 ctgcaccgtt tccaccgata tggacggcat ctcgctgctc tccttgctca accgtatcga
     5461 ggcggatttc gggcgcaaac gcagtttccg aaacgcgccg cgcacattgg atttggacat
     5521 tatcgacttt gacggcatct ccagcgacga cccccgcctc accctgccgc atccgcgcgc
     5581 acacgaacgc agtttcgtca tacgcccttt ggcagaaatc ctccctgatt ttgttttagg
     5641 caaacacgga aaagttgccg aattgtcaaa acaactgggc aatcaaggta tccgtctttt
     5701 accggacagg taattccgca cacagatgcc gtctgaatgc cggtttcaga cggcattttt
     5761 accctgccgc caatctgtac gcaaaaaaaa tcgccccttg aaaaaggggc gcaaaaggaa
     5821 cacaaaccac taccaaaact ttaaatctgc aacactgcct gccgcatact gtatccgaca
     5881 ggatataaag ccctcaccaa atcgtttcga gaaatccaaa cttcttcatc gccgacagaa
     5941 aacctgcctt tctccggcac cagctccaac aaaaacggct gaaccgccgt atgcagcttg
     6001 tccttacacc gccccagctt tctggacaac gcggacaagg cacgcttata gacactatcc
     6061 ttgcggtcaa gttcccgcgc actgccgacc cagctcaaac gaaggttgcg gctttcatcc
     6121 tcaatcagca aaatgcctgt tttgacttcc cccacctcgg gctcacatga aagcgcaata
     6181 taatatttgc cgtcgatgtt gacatacgcc gcttcatgca cctgacagtt gcagccggaa
     6241 gtggcaagca gcgaataaac agagtcccga tgcagtttca aaatatcaag ggaaatctgt
     6301 ttgaaaatac tttccggcat ttttccctcc tttcgtctgc tgcgttgttt actggattga
     6361 ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt ttttaaaaaa
     6421 tttataaatt attgatggat atgtgaatta attttaagat ttaaaatctc tttctcaaaa
     6481 aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag ccagtccgtt
     6541 taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg ataatccacg
     6601 ctgtcaaagc ccgcatccag catcatctgt ttcaaagttt cctgatcggg gtgcatacgg
     6661 atggattcgg cgagatattg ataactctcc gcatctttgg caatcagttt gcccatgacc
     6721 ggcagcagct tgaacgaata gaaatcatac gcgccttcca aaggtttgta gattttggaa
     6781 aactccaata ccagcaatgt cccgccgggt ttcaaaacac ggtacatctc tttcagcgcg
     6841 gcatctttat gcgtcatatt ccgcaaaccg aacgccacgg aaaccaagtt gaaataattg
     6901 tcagggaaag gcagtttttc cgcatcggca agcgacacgg gcaaaatcat gccttcgttc
     6961 aaaagacggt cgcgcccgac ggtcagcata gaggaattaa tatcggtcag ccaaacctcg
     7021 ccttccttgc cgacccgttt cgcccaaccg cgcgacaaat cgcccgtacc gcccgcaatg
     7081 tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg cttccacacc
     7141 ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc gacggagtgg
     7201 aacacttccg ccactttgcc ggctttctca tcttcgttga ccgtactgaa tccgaaatgc
     7261 gttttctgtc cgcccatatt ctaaccacct ccctgatgcc gccctacggc atcccaaact
     7321 taacaatctg attgcttttc aaaacgggaa gcacccgccg ccgccaattt gtccaaatat
     7381 tcctgccaca tcgcatcgta tccgtatgcc agtctgtgca gatacgccca atcgtaaaga
     7441 ccgctgtcgt gcccgtctga aaaactgatt ttcagcgcgt actgtccgac aggctgcaaa
     7501 tccgcgattt ggacatccgc cttgccggtc tgcaaaacat cctgtcccac gccgtgtccg
     7561 cgcacttccg cactcggcga atagacgcgt aaaaattcgg caggcagatt tttaggttcg
     7621 tccccataag tcaaaaccaa cacggtacgg tttttcaaca aacggatttc aacaggaata
     7681 ttattcaaaa aacacctcgg ttcggcatac aatatccggc aggcgcaggc gtgccttcaa
     7741 acggcgcgta ttttaacagc tttatcccga taccgatatt caaacccaca ctttaaacgg
     7801 cattgatatg gacaatctcg tatgggacgg cattcccgac atccgcacac tcgaccaagc
     7861 catccgcaaa cacgcacccc cgctcaacct gattatctgc ctccccgata atcagattcc
     7921 cgattttcaa accgcacaag atgcttcgga cgcggaatgc cgtctgaagc accgtttgga
     7981 tcaggcaacg cagtgcctcc agttcgacag catcaacctc atcgaacaca tcctgcccga
     8041 tgtccgcttc tggctggttc ccccttcacg cacccgccgc ctgcacgaac acttccacca
     8101 catttcctgg cagaccgaag ccatcccgca aaccgaaagc aaacccgaca aaccctggtt
     8161 tgcacttcca caaacatccg aacggcaaaa accggaatac atcctcgtta tcggcgcggg
     8221 catatccggc gcggcaaccg cccacgcctt agcatcacac ggcatttccg ttaccgtatt
     8281 ggaagcccga aaagccgccc aagccgccag cggcaaccgc caagggctgc tctacgccaa
     8341 aatctcgccg cacgacaccg aacaaaccga actgctgctt gccggctacg gctacaccaa
     8401 acgcctgctc ggacacatcc tgcccgaatc cgacacttgg ggcggcaacg gcatcatcca
     8461 cctcaattac agccgcaccg aacaacaacg caatcacgaa ttgggtttgc aaaaacacca
     8521 taaccacctc taccgcagca tcacgcaggc agaagccgaa aaaatcgccg gtatcccgct
     8581 gtccgtccca tacgaccacc cttcatgcgg actctactgg caacacggcg tatggctcaa
     8641 tccgcccgca ttcgtccgcg ccctcctccg ccatccgctg atcggactgc acgaagacac
     8701 accattaacc gacatttccc acgacgggga aaagtggatt gcaagcacgc caaacggcac
     8761 atttaccgcc acacacatca tctactgcac cggtgcgaac agcccctacc tacccgaaac
     8821 caacctcgcc gccctgcctc tcaggcaaat acgcggacaa accggcctca caccgtccac
     8881 cccgttttcc gaacaactgc gttgcgccgt ttcaggcgaa agctacatca gcccgtcgtg
     8941 gcacggactg cactgctacg gcgcgagttt tattcccaac agcagccata ccggatggaa
     9001 cgaagccgaa gaagcctcaa accgccaagc attggcacac cttaaccccg ccctttccga
     9061 atcattgttt gccgccaacc caaaccccca aaaacaccaa gggcacgccg ccatacgctg
     9121 cgacagcccc gaccaccttc ccctagtcgg cgcactcggc gacattgccg ccatgcggca
     9181 gacctacacc aaactcgcgc tggacaaaaa ctaccgcatc gacaccccat gcccatacct
     9241 gcctaatgcc tacgtcaaca ccgcgcacgg cacccgcgga ctcgccaccg cccccatctg
     9301 cgccgccgcc attgcagccc aaatcctagg cttgccccat cccttttcac aacgcctgcg
     9361 ccacgcccta caccccaacc gcaccatcat ccgcgccatc gtcagaagga aggatctaac
     9421 cccttaagcc tttgccgcat gccgtcccga ccacagaaaa tcatattcaa ataaacatac
     9481 caatcctgac ggtaaaatat cgaaaacacc cataacgttt tcttcccaaa ataaggagta
     9541 aggaatgcct aacaaattgc ctttgctgtc caagatgaat gttccgtaac attcgaagac
     9601 ttccttaacc ctgccagccg tttcggtttc tgtaccccct gttagataag gaaggaatga
     9661 aaaagccctg ttgattgtat gaagtaaagg gcgttaattt ttgttaatct tcccttctca
     9721 ggaactcaat atataaggat gttaattttt atttcccttc tcgagg
//
LOCUS       DAWKOD010000064         9753 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000064 DAWKOD010000000
VERSION     DAWKOD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.064"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(35..760)
                     /locus_tag="WJK73_001172"
     CDS             complement(35..760)
                     /locus_tag="WJK73_001172"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ7557655.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVVFPDRLHIPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(830..1264)
                     /gene="fur"
                     /locus_tag="WJK73_001173"
     CDS             complement(830..1264)
                     /gene="fur"
                     /locus_tag="WJK73_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ7557656.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1483..1860
                     /locus_tag="WJK73_001174"
     CDS             1483..1860
                     /locus_tag="WJK73_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7557657.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1870..2679
                     /gene="dapB"
                     /locus_tag="WJK73_001175"
     CDS             1870..2679
                     /gene="dapB"
                     /locus_tag="WJK73_001175"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ7557658.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDTGKAAIHTAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2943..3353)
                     /locus_tag="WJK73_001176"
     CDS             complement(2943..3353)
                     /locus_tag="WJK73_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557659.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            3576..3965
                     /locus_tag="WJK73_001177"
     CDS             3576..3965
                     /locus_tag="WJK73_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ7557660.1"
                     /translation="MNVKIKYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(4009..4167)
                     /locus_tag="WJK73_001178"
     CDS             complement(4009..4167)
                     /locus_tag="WJK73_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557661.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(4175..5347)
                     /gene="lpxB"
                     /locus_tag="WJK73_001179"
     CDS             complement(4175..5347)
                     /gene="lpxB"
                     /locus_tag="WJK73_001179"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ7557662.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(5395..6387)
                     /locus_tag="WJK73_001180"
     CDS             complement(5395..6387)
                     /locus_tag="WJK73_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7557663.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6881..9640
                     /locus_tag="WJK73_001181"
     CDS             6881..9640
                     /locus_tag="WJK73_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ7557664.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTARQEARADTAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RSQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDTAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         2516 a         2994 c         2276 g         1967 t
ORIGIN      
        1 ccatatttga acctgaccgg agcataagcc gccatcaaag ccccttgcac gccacttcgc
       61 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
      121 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtatcc tgctggcagt
      181 ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
      241 acgcatccgg ttgtaatgcg aacatcgatt cgccataaaa caccctgccg atctgaacgc
      301 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaaag
      361 aatgcgcgta ccccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
      421 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
      481 agccgttgac cgcaacccga tagctgccgt tgcgcagcgt tttcgccagc gagcgcggaa
      541 tatgcagcct gtcgggaaac accaccgcac ggggcccgac cgcataccaa aaaaaccacc
      601 cgtcccggga aaaccacgga aacacgccgt tccgatacgc ctcaagcagc cgccccgcat
      661 ccaaatcgcc gctcacgccg accagcccgt cgcaccgggc caaagcatag gcaggatcgg
      721 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat gacagttctc caaaaacaca
      781 ggcggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
      841 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
      901 agccgttttc ttccgcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
      961 ttacctcgcc gcacttcacg cagacgatgt ggtcatggtg gtcgcctttg tccaactcat
     1021 aaaccgcctt gcccgtttca aaatgatggc gttgcaaaat gcccgcctgc tcaaactggg
     1081 tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     1141 acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc aaaatcttca
     1201 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     1261 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     1321 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     1381 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     1441 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     1501 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     1561 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     1621 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     1681 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     1741 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     1801 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     1861 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     1921 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttctggtgc gcttgaacac
     1981 tcaggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     2041 gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat cgacttcacc
     2101 cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     2161 atcggcacaa caggcttcga cgatacgggc aaagccgcta tccacactgc cgccgaaaaa
     2221 acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt ccacatcctc
     2281 gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     2341 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt catcgccggc
     2401 gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca caccggtccg
     2461 cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     2521 accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaggc cagcagccgc
     2581 atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg tcaacggcaa aacgggtttg
     2641 tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa aatgccgtct
     2701 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atcaatgttt
     2761 caacacacag gacgacacat aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt
     2821 acgcccctcc caaataaaat ctgattctac cgccccgaag gacagatgtc cgagtggcgg
     2881 ggtttcaacc gaaaaggaaa tacgataaag ttgcctgctc agcataacaa gctacggctc
     2941 gtttaattga aactctcctg atctaaaaat tctaactcta tttcccggca aactatatct
     3001 atactaaaac cggtcggaag atcataccta ctgcctatca cttccagcgc acctccataa
     3061 tccaagattt catcatcctg ctttaattct ccaagttcca atacattttt gatattttta
     3121 tcttggttct tcgtggcgta tccatagata aatttcagat agctgaactg cttgaattta
     3181 atttttaccc agtctgtata atttggtgta ggaatctctt gaggaaagtc cggataatcc
     3241 acattctgga cgatgatatg gaattcatcg tttttcaaag tatagtttaa tagagtgccc
     3301 tcatatatat acagaaattg ataatctttt tttggaatgg tataagttgt catattattt
     3361 taccttttca aaaacaatga gtttaagatt ttttgagtcc aagttggttc tactactctt
     3421 atgaaatttc agttgtttaa actcttcctt tattttaagg aatttttcaa ctagccgaga
     3481 acaaactgga gcaaaatgga cgattgagtt taggaatcaa cctgattcag tacatgggca
     3541 aagattagaa ttaaaattta gataaaggtt acgttatgaa tgtgaagata aaatataatc
     3601 ttttagaata ttgtgaatat ttaggtggta tttttggtgt ttataaaaat tatttaaata
     3661 tggatatcaa agatccaaat ttaaaaatta gattttttga ttttttggaa gaacttttat
     3721 cagatggtgt tattgaatta tgcgattatc gtgaaaatcc tccaaaaata ttaactggtt
     3781 cgccaaaaac acaggtagat gaattaagaa gaatttggcc ggatatggaa gagatgttgc
     3841 tatattttcc agataatcct tggttttatg tagaacattt ttggtgggga gcaacttgcc
     3901 ctattgaact gacacaattg cctaaaatag aaatttacga agaacaaatg aaacaaggta
     3961 agtaagcgta ttagtataag ctataatcat aaatcatata taacaaaatt aatcagttaa
     4021 ctgaccatta tattttaacc ttaatcccat tcgggccagt tctgtttcat ttggaattcc
     4081 tcttaaatac ttattgtttg atgtgccatt aatcacaatt tctccccctt tttttgtaat
     4141 acgtgctgct tcgggaaaat aatccattaa ccgctcaaca tcccgcctct tccaaaaccg
     4201 cgcgcgcggc caaatccgcc gtatcttttt tcaacagcag gtgcaacgcc ctgaaatcct
     4261 gttgcagcgc ggcaacctta tcggggtgtt cgtaccagtc cgccaacgcc gccgccagtt
     4321 tttccggttt tgcttcagat tgcaataatt ccggcacagc ctccttaccc aacaggatat
     4381 tcggcaggcc gacatgcggc actttgattt tgcgtttcac ataagcatag gtcagcggcg
     4441 aaatcttgta gctgatgacc atcggacgct tacacaacgc cacctccaaa gttgccgtac
     4501 cgctcgttac cagcaccgca tccgccgccc tgcacactgt ttcagactgt ctgtcgatta
     4561 ccgtcagcgg caatccggca aactccggcc gctgcaaaac ttccgccaaa cgccgcttcg
     4621 tcgcctccgt tgcggcaggc agcaggaagc gtgcggcggg atagcgttcc aacaacaata
     4681 atgccgtctg aaaaaacacc ggcgccatat agtcgatttc gctgacgcgg ctgccgggca
     4741 gcagggcgaa tacggggatg ccggcatcca cgcccaaagt ttgccgcgcc gtttcacggt
     4801 cgtcttccaa gggcataagc tgcgccatcg gatgaccgac aaactccgca cgtccgcccg
     4861 catcgagata aagctgcggc tccatcggga acaggcacaa cacgcggttg acctgatgca
     4921 cgattttgcc cacacgttcc cgccgccacg cccacaccga cgggctgaca taatgcacgg
     4981 tcggaatccc cgaccgtttc agcttttccg ccacacccaa attaaaatcg ggcgcatcga
     5041 taccgacaaa gacatcaggt ttcaacgaca gcaaatcccg taccagcccc ctgcgtatcc
     5101 gtaaaatttc cggcagccgc ctgaccactt cgacaaagcc gcgcaccgcc agccgctcct
     5161 gatcataaag gctctcgaaa ccttccgcct tcatcagttc gccgccgata ccgacaaaac
     5221 gcgcctgcgg acaacgcttg cggatggcgc gtatcaggtg cgcccccaat aggtcgcccg
     5281 acgcttcgcc gacactgacg gcaatcaaag ggcttttttt atcagccata ttcgtctgtc
     5341 cccacatact ttcacgtccc gatgccgtct gaaggcttca gacggcatcg gggttcacat
     5401 tttcgtcccg ttttccaaca tcaccgcaaa ctgcgccaag tccggcggca gctccgcctt
     5461 caacaccagc ggctcgcccg tgagcggatg gttcaagtgc agctcggacg cgtgcaaaaa
     5521 catccgcttc aaacccaact tctgcaaacg acggttcgcc tgataatcgc cgtagcgttc
     5581 gtcgcccgca atcggacagc cttgagattg caggtggacg cggatttggt gcgtgcgccc
     5641 cgttttcaac gtcgcccgca ccaaagtcag gtgcgacagc ccgacaccgt gcaaaatgcc
     5701 gtctgaaaaa cggcttaaca cacggaacac cgtatgcgcc gactgcccgt cctcgctgac
     5761 gcgtaccatc ttttcgcctt gtgcgccggt atatttgaac aggggcagtt tgacatggaa
     5821 attgtcgtcc ggcagtttgc ccacccccag cgcaaggtag atttttttgg ggtggtcgtt
     5881 acggatggct tcgtgaagtt tgacgagcgc gctgcgtttc ttcgccacca tcaacaagcc
     5941 gctcgtatcc ttgtccaaac ggtggacgag ttcgagataa cgcgcctccg gacgggcgcg
     6001 gcgcaactgt tcgataacgc cgaaactcac gccgctgccg ccgtggacgg caacgccgga
     6061 cggtttgtcg atgaccaaaa gcgcatcgtc ttcgtaaaca acgtcaaacg cacgcgccgg
     6121 tacggcggca cgcctttcag acggcatttc cttctccgcc acgcgcacag gcggaatccg
     6181 caccgtatcc ccctccgcaa tacggctgtc gggtttgcag cgtttcttgt tcaaccgcac
     6241 ctcgccggcg cggataatgc ggtggatatg gctcttggga acacccttga ggatttttat
     6301 cagatagtta tcaaggcgtt gacccgcctc atgttcggca acccctatca agctgaccga
     6361 atctttgctt atttcgtgcg ttttcatcta taatccgaac atccgtttca gcaaaaagcg
     6421 cgcccgccgc gcccccctga aacggtttac tttaaaacgg aattttatat taaaaacgca
     6481 ctccgcaaag cattttccct gcgcctgttc ccagccggca ggcgcagaac gtaatcaagt
     6541 ttgaattgat tttgccgttt cggcgcggaa gtaagacggc agccgggccc caagtcccaa
     6601 acgcaaaccg cccaaaacac aggcatcacg ataacaagaa gaagccggcc cccatttttc
     6661 cgaccgcagc gttcggaacg cggcagacaa accgccgcat cgatctttat agccttctgt
     6721 atccgaccct tccgcacacg gttctccgga acgtgcatcc ctgcggattt tcaactcaaa
     6781 aactgattac cggttttatc cgaagacatc ggaaaaccca gccttacgga atgccgaacc
     6841 ggaccggcgc gcccgaatgc cgcccgtcca cgaggtgatc atgaaaagaa tgttattcaa
     6901 cgcaacgcag gccgaagagc tgcgcgttgc catcgtcgac ggacaaaacc tgctggattt
     6961 ggacatcgaa acgctgggca aagaacagcg caaaggcaat atctacaaag gcatcattac
     7021 ccgcatcgag ccgtcgctgg aagcgtgttt cgtggattac ggaaccgacc gccacggttt
     7081 cttgccgttt aaagaagtct cccgctcata cttccaagac tacgaaggcg gacgcgcgcg
     7141 catccaagac gtgctcaaag aaggcatgga agtcatcgtc caagtcgaaa aagacgagcg
     7201 cggcaacaaa ggcgcggcgc tgaccacctt catcagcctc gccggacgct atctggtatt
     7261 gatgccgaac aacccgcgcg gcggcggcgt atcccgccgt atcgaaggcg aagaacgtca
     7321 agaactcaaa gccgctatgg cgcaactcga cattccgaac ggcatgagca tcatcgcccg
     7381 taccgccggc atcggccgca gcgcggaaga gttggaatgg gatttgaact atctcaaaca
     7441 actctggcag gcgattgaag aagcaggaaa agcgcatcac gacccttacc tgctctttat
     7501 ggaaagctcg ctgctcatcc gcgccatccg cgactatttc cgccccgaca tcggcgaaat
     7561 tctggtggac aatcaagaag tttacgacca agttgccgag ttcatgagct acgtcatgcc
     7621 gggcaatata ggccgtctga aactctacga agaccacacg ccgctgtttt cccgcttcca
     7681 aatcgaacac caaatcgaaa gcgcgttttc gcgcagcgtc agcctgccct ccggcggcgc
     7741 gatcgtcatc gaccataccg aagccctcgt ctccatcgac gtgaactccg cacgcgccac
     7801 tcgcggcgca gacattgaag acaccgcgtt caaaaccaat atggaagccg ccgaagaagt
     7861 cgcccgacaa atgcgcctgc gcgacttggg cggcttggtc gtcatcgact tcatcgacat
     7921 ggaaaacccc aaacaccagc gcgatgttga aaacgtcctg cgcgacgcgc tcaaaaaaga
     7981 ccgcgcccgc gtgcaaatgg gcaaactttc acgtttcgga cttttggaat tgagccgcca
     8041 acgcctgaaa ccggctttgg gcgaaagcag ccatgttgcg tgtccgcgct gcgccggcac
     8101 cggcgtgatt cggggcatcg aatccaccgc cctgcacgtt ttacgcatca ttcaagaaga
     8161 agcgatgaag gacaacaccg gagaagtgcg cgcacaagtg cccgtcgatg tcgccacctt
     8221 cctgctgaac gaaaaacgcg ccgagctgtt tgcgatggaa gagcgtttgg atgtcaacgt
     8281 cgtcctgatt ccgaacatcc acctcgaaaa tccgcactac gaaatcaacc gcatccgcac
     8341 cgacgacgta gaagaagacg gcgaaccgag ctacaaacgc gtcgccgagc cggaagaaga
     8401 cgaatccgcc aaaccgttcg gcggcgaaaa agccaaagcc gcccgtcccg aacccgccgt
     8461 caaaggcgtg cgccacacca gccccgcccc gactgccgcc cccgagaaaa aaacctcctg
     8521 gtgggacagc ttcaaagctt ggctcaaacg cattttcggc ggcagcgaaa cccaagccgc
     8581 acccgctgcc gaaacctccg aaaaacgcag cacggcaaac cgcagcggca gccgcgccaa
     8641 caaccgccgc caaaacccgc gccgcagcaa acgcgaaggc agcaaagtag aagtccgcga
     8701 agtggcaggc aaaactgcca gacaggaagc gcgtgccgac acagccgaaa cgcgcaacaa
     8761 cggcaaccgc cgccgcaacg aacgcggcga ccgtgccgcc gaacgtgcca acgaagcgga
     8821 aatccaaagc cgcaacgtac agcctgccgc aaccgttgca gatgccgcac cgtccgaaac
     8881 cgaagtgcaa accggaaaac gccgccgcaa cggcagccgc agcgaacgca gccaaaccgc
     8941 gccggaaacc gccgccgttg ccgaaacaac cgttcagaca gcggaaaaca cgccgtccga
     9001 accgcatacc gcagaagaca aaggcagcaa gcccaaatcc gaacgcaacc gccgcgaacg
     9061 cgacagccga gatgccaaag aacgccgcga gcgcaacaat cagcgcgacc gccgtcaaaa
     9121 cggcaaaaaa cgcaatattc cgtctgccgc caaaatcgag cagtacctga atattcacga
     9181 caccgccgac aaagtccgtt ccgccgccgc gcacgttttc ggcgaaaccg acgcaaacgc
     9241 gccgattacc gtcagcattg ccgatccggt tgcagaaagg gatcttccga cagcatctcc
     9301 cgccgtttca aacggcgacg caccggttta tgatacggcg gaaaaaatcc gccgtgccac
     9361 cgccgccatc ctgcccgaag gcgcgacacc gaaagccgaa gcacaggaaa tgccgtctga
     9421 aaccgcaacc tttacggctg cggcggaaca ggcacgggaa accgcacaaa ccggcggact
     9481 cgtcctgatc gaaaccgacc ctgccgcatt gaaggcatgg gcggcacaac ccgaagtcca
     9541 agccggacgc ggtttgcgcc gttccgaaca gcccaagccg tctgaagccg caaccgtccc
     9601 tgccgaagaa atgatccaag tcgaaacccg gcaaggctga accgacggcg gcaaaaagag
     9661 gttctgttcc gcagaacctc ttttttacat gggttcggat acctgcaatg ccgtctgaaa
     9721 cttcgccatt cccgtgatta ccgaaacatt ccg
//
LOCUS       DAWKOD010000065         9648 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000065 DAWKOD010000000
VERSION     DAWKOD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.065"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(5..217)
                     /locus_tag="WJK73_001182"
     CDS             complement(5..217)
                     /locus_tag="WJK73_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557665.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(384..1097)
                     /locus_tag="WJK73_001183"
     CDS             complement(384..1097)
                     /locus_tag="WJK73_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ7557666.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSSSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFAVESLTEL
                     SNDDLQKTYAAVSTKKRKK"
     gene            1330..1581
                     /locus_tag="WJK73_001184"
     CDS             1330..1581
                     /locus_tag="WJK73_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557667.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            1583..3560
                     /locus_tag="WJK73_001185"
                     /pseudo
     CDS             1583..3560
                     /locus_tag="WJK73_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3627..3704)
                     /locus_tag="WJK73_001186"
                     /pseudo
     CDS             complement(3627..3704)
                     /locus_tag="WJK73_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3775..4689
                     /locus_tag="WJK73_001187"
     CDS             3775..4689
                     /locus_tag="WJK73_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ7557668.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMQDSKARREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDEELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            4780..5046
                     /locus_tag="WJK73_001188"
     CDS             4780..5046
                     /locus_tag="WJK73_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557669.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            5155..5328
                     /locus_tag="WJK73_001189"
     CDS             5155..5328
                     /locus_tag="WJK73_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557670.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            5321..5488
                     /locus_tag="WJK73_001190"
     CDS             5321..5488
                     /locus_tag="WJK73_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557671.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            5552..5779
                     /locus_tag="WJK73_001191"
     CDS             5552..5779
                     /locus_tag="WJK73_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557672.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            5794..6312
                     /locus_tag="WJK73_001192"
     CDS             5794..6312
                     /locus_tag="WJK73_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ7557673.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(6472..6687)
                     /locus_tag="WJK73_001193"
     CDS             complement(6472..6687)
                     /locus_tag="WJK73_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557674.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(6691..7299)
                     /locus_tag="WJK73_001194"
     CDS             complement(6691..7299)
                     /locus_tag="WJK73_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ7557675.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            complement(7431..7631)
                     /locus_tag="WJK73_001195"
     CDS             complement(7431..7631)
                     /locus_tag="WJK73_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557676.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(7832..8017)
                     /locus_tag="WJK73_001196"
     CDS             complement(7832..8017)
                     /locus_tag="WJK73_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557677.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(8290..8457)
                     /locus_tag="WJK73_001197"
     CDS             complement(8290..8457)
                     /locus_tag="WJK73_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557678.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            8694..8936
                     /locus_tag="WJK73_001198"
     CDS             8694..8936
                     /locus_tag="WJK73_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557679.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            8980..9171
                     /locus_tag="WJK73_001199"
     CDS             8980..9171
                     /locus_tag="WJK73_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557680.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            9168..9584
                     /locus_tag="WJK73_001200"
     CDS             9168..9584
                     /locus_tag="WJK73_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557681.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         2663 a         1946 c         2494 g         2545 t
ORIGIN      
        1 ggatttaatc aatactccgg ccataccact caacacggcc tatgatggcg atgtcgtctt
       61 gggtattact caaatctatt tcaaacggtg cgtaacgtgg attttcagac gttacaagca
      121 gtttgcccgg tatacgttgc acacgtttga caaagaggtc attgcctata cgcaagacat
      181 ataggccgtc gcgcggctcg gtttcggcgt ggttgattag aatgtttata aaaggcttta
      241 actagaaact gaacatggaa cttgtataat agttccatgt ttgaaaaaat cttttaatat
      301 catacatcta aaaaaacaaa acatctgaag accataagct aaacatgaaa cttgaattta
      361 aaggcggagt ttcatgttta agcctatttt ttccgttttt tagtcgatac tgctgcatat
      421 gttttttgca aatcgtcatt acttaattca gtcaggcttt ctactgcgaa atttttttcg
      481 aggtaacttt tcaaccaatc ttcaagctca agttgtttta catttaattt gatataagcg
      541 tatttctttt ttcgccagtc gggatcgttg ttctttgatg ttgctgtatt gctcaaccta
      601 cccagccatt ttctgagata agtttctgct ttgtcgtaat cgctcaatgc gattagtttg
      661 taacttggaa ctttacagtg agcattcaga ctagcccaaa ttgcacggat tgattttggc
      721 gaccgtttgg caatctcttc tgctgcggca acttgtttaa caagttgttg cagctttgaa
      781 gcttgctcaa gcgttatatg ttccttgccg ggttttgaat cgacacgggt tctgatgacg
      841 tgattttggg tattaatttg atgaacaacc gaactagatg aggcgataac agcgttactg
      901 acgttgccgt taatctgatt ctcaacgttt gatttgccaa tagcgtcgtc caatttttta
      961 ttaaggtcgt ctgaaacagc atttaaattt gccgaatgaa tgcctgtaaa gacaaattga
     1021 atatccaccc ctaattggac agcagcaagt aaaaagtccg caggaatgtt gacctgcccg
     1081 ctttcataca gtctcaaact ttctgcactg catccagttt gctcagcaaa attctttcga
     1141 gagtatccaa gtcggcttct ctctttaact aaattcaatg ctaaatcaat gcgttccata
     1201 aaacaccgca aaatagtacc caatatattt ggtataccaa atatattggg tatatcatcc
     1261 aggtaacagt taaggcaggc attaaagcct gtaaggtaag taagtaatgg attctatcat
     1321 ggaggcatta tgcaagcgaa cgaaatctat aaatccttag aagaaaaaaa tatatcggca
     1381 agaatgttgg cttcggcttt aggggtaact aaccaatctg tttctgaggt cataagaaat
     1441 ggacgaggaa gcaaaagaat tgcggaggca atcgcgaaag tgattcaaaa agatttagtt
     1501 gaagtttttc cgcattacaa gaaaacagat tgtcgagaca aaaagatagc tgaattgaaa
     1561 gagatgctcg gagtgggttg aaatggaata tatggatctc aaaagcctgc ttaattttgg
     1621 gttgccaaat atgccaagaa cagtaatagg gctgagaaaa aaaagcgcaa agggatggtt
     1681 ggcaaacgag aaggcgtcaa ggtaagggtg gtggtgttga atatgcccta cctattgaaa
     1741 tccgagcagc catcatgaaa cggcagtcgg acgagctggc ggaaaagatg ccgaaaatgt
     1801 tgccccaagt cagaccgggg acggcgatgt cggctcaggc actggctgaa gcggccaagc
     1861 tgttgaacga gaaacaacgg tcggtggcgg atgcgcgatg tgcggtggtg gcggcggtat
     1921 tggggattaa ataccaatac ggttgctctg caaaggctgc ggtggctcag tttttgggct
     1981 tgctggcaga aagtaaattg gacgcggtca cgcttgggaa cttggaaaag gccaatgacc
     2041 gcagccggac ggcaaaggtt ggcgaacgta ctttagacgg ctggatttct gcttatttga
     2101 aagcggaaaa cgcgacggag cggttggttg ctttggctcc gaagacgacg aaggcggtca
     2161 agccgattga gagctacggt tggttgccga tgtttatgca gtttcacaat atcccttcgg
     2221 ctccgaaact ggcgcacagc taccgccggt ttgtgcagtg ggcggaagcg gaaaatatgc
     2281 cggtcaatga tgtgcctaac ttgagtatgg tgcggcgcgt ttgggacaag ctcccgctga
     2341 ttatgcagga gcgcggcagg aaaacggggg cggcttataa atcgctgttg ccttatgtga
     2401 aacgtgattg gggggctttg aagccgaacg atgtttggat cggcgacggc catagcttta
     2461 aggcaaaggt ggcgcatccg gtacacggca ggccgtttaa gccggaagtg acggtgatta
     2521 ttgatggttg tacgcggttt gtggtcggtt tttcggtgtc gttggctgaa agttgtgtgg
     2581 cggtatcgga cgctctgcgt atcggggtca agcactttgg tttgccgatt atctactact
     2641 cggataacgg cggcggccaa accggcaaga cgatagacca tgaaatcacg ggtattacgt
     2701 cccgattggg tatccgccat gaaacgggta tcgcgggcaa cccgcaaggg cgcggcatca
     2761 tcgaacgatg gtggaaagac aatctgattg agatggcgcg acagtatgag acgtttgcgg
     2821 gcgcggggat ggacagcagc acgaagaacc tgatgtaccg caagatggaa agtgctttta
     2881 atgctttgga aaaaggcaag gagttgacgg tagaacagca aaaatatttg aaaaaactgc
     2941 cgagctggtc gcgttttatc gcggatgtgg ccaagtgtat cgacgaatac aacaaccgcc
     3001 cgcacggcga gctgccccga catcctgacg gcgggcatta ttcgccgaag gcttatcggg
     3061 aaatgaggct ggaacaggac ggtatcgcgc cggatatgtt gtcggcggaa gagctggcga
     3121 cgatgtttat gccgcaggag gtgcgaaaag tacagcgcgg ttggctggat ttgttcaaca
     3181 actcttattt ctcaaccgag ctggcggagt atcacaagga cgaggtacgg gtcagctacg
     3241 atttgagcga tgcgtcggcg gtcaatgtgt ttgatatgga cggcaagttt atcactaagg
     3301 cgcaggccaa cggcaatacc cgcgaggctt tcccgacggc tcgtatcgac caactggcgg
     3361 aaaaccgccg aaaaggcaaa atcaagcggg cggaaaatgc aatcaagctc gcaaatgcgg
     3421 aagtcaatcc ggcactggaa caggctgcgg tttgggacga gctgggacat ttgggcggaa
     3481 acgtcatcga ggcggagtat gcggtattgc cgaaaacggg cacggacgac gggattttct
     3541 tgtttgaggc ggatatgtaa aggaaaacat gatggaaaaa cagaaaaatg cggcgttttc
     3601 ggccgagctt gttgaaaaat tgaaactcaa gcgagccctt gggcggattc aacgatttca
     3661 agcaaagatt caaggtgttc ccgctgaacg gaatcaggct caaatttcta aatcgggaaa
     3721 ctctttcatt tcagacggcc tttaaaggtt gtttaaaact caaggatatt aaaaatgaaa
     3781 caaattaatc aagcattgca acaaaaactg gttgaattta aagaaaaatc gggtatgaac
     3841 caaacccaac tggcacgcgg tatcgatact tcaccggcct ccatcagtat gtatctgaac
     3901 ggcacttatg ccgataaagg cggcaattat gaaaccatcg agacgaaaat cgaggcgttt
     3961 ttggagatgc aggacagtaa agcgcgacgt gaagagctgg tgttgggatt tgtatcgact
     4021 aagacgacac gccgtattgc ggaagcgatg cgcgatgcgc acgaaggcgg cgaaatagtg
     4081 gtgatctacg gtcaggccgg tttgggcaag actcaggcgg tcaaaaacta ctgcgagaaa
     4141 aaccctgcgg ccatcttgat tgaggctaat ccgagcttta cggctttggt cctgatgcgc
     4201 aagttggcga cggcggcgaa ggtatcggcg atgggcagcc tgaatgattt gtttgaatcg
     4261 gtatcggaca gattgcgcga ttcgggccgt ctgattgtgg tcgatgaagc ggaaaacctg
     4321 ccgttacgcg cccttgagat tatccgccga ctgcatgatg aaacgggctg cggtttggtg
     4381 ttgagcggta tgccccgatt ggtggcgaat ttgcgcggta agcatggcga gctggtacag
     4441 ctttacagcc gcgtgtcgct tccgctgaat ttgggcgaat ctttgccgga tgaagaactc
     4501 tttgagattg cgaaagcggc tttgcctgat gcggacgagg agacgctctt ggaactggtt
     4561 aaacatagta acggcaatac gcgccggatg agcaaattga tgcgcggcgc ggtacgcacg
     4621 gcgaacaaga acggtatcaa gatgcaggcc ggtatcgtta aaaaatacag ctccctgatt
     4681 atccgatagg gccgtctgaa acggtaagtc tctgacaggg ctatatattt ttttacccta
     4741 tgattttaat aagttgttgt tttaaaagga aaacgcaaaa tgcaagtttt gaagaaagtt
     4801 gattggaaga tgtttgtggc acgctctttt tggcggtgtg tgccggttgg tttgacggcg
     4861 ggcgtgtggt gttttgtggc ggggatggcg ttgcattcct gcgcgaaaga acctgaaccg
     4921 gttgcgaaag agctgacgaa ggtcgagaag atggaaagac aggcggattt ggaggtcaag
     4981 gtatgggaag agcaatacga gtcaatgagc gtcgaggaaa aaatgaaagg gattgtttat
     5041 gagtgataag ccaattttat tgagtcctgc ggcgaaaaaa gaggctttgg atcgggcggt
     5101 aaaggaaatc cgtgcaaaat atggcgataa ggcgattgtg aaaggatgtg tgaaatgagt
     5161 ttcggacgac gtaatacgga ttggcaggct tggggacaac accgcaggcg tgcgacggcg
     5221 cgaatggcgc aaaaaagccg ggagcgcgaa atcgaggaat atcaggcgca gtttgaagat
     5281 aaggacggca aaggtcgtct gaaaacggga ggaaacaaag atgaatgaaa aagatttgat
     5341 cgaatggctc gaagaccgtg gcgagctgat ggtcatgaag aaggacggcg aaggctttgt
     5401 gattgcagcg cgtgcgccgg acgggatgtg gaagactgcc gaggcggaaa ctttggctca
     5461 ggcgataact ttatgggagg aagtgtgatg tttaagtttt tgagaaaccg cgcgaaagtt
     5521 gaagcaattt taaacaagaa aggaaagtga aatgaaagtg tatgttttta aaattagtaa
     5581 tgaaaacggt aagttaaaga tagaactccc cgaaatccca atgggtaagc aaattgacga
     5641 agttgatttg attgctgggc taaccacgga atttattgca agtatgttac gtgatgccca
     5701 aaaagatcgt cgcaaattcg taatcgacgc atcaaatcaa ttggctgcaa tccagacata
     5761 tcaaaaaatc ttcaattaag gaaggaagag aaaatggcta aaacccgaat caaacagccc
     5821 gccatcgaag cggcacaaga caaaacggaa gttactgcat ttatccgcca aatcggcgac
     5881 ttgcagcgcg aagtcaaacg cctggaaacc gaagccggag acaaaaaagc ggctatcgaa
     5941 gaagaatatg ccgccaaagc cgcgccgatg tgtgccgaaa tcatgagcct gaccgaacgt
     6001 gtggccgcct actgcgaagc gcataaggac gagctgacgg aaaatggtaa aaccaaaacc
     6061 gtggacttta ctaccggcct gattaaatgg cgcattcgtc cgccatccgt caaggtaacg
     6121 ggcgtggccg ccgtcttggc gtggctctcg gaaaaatccg cctttgccga gtttgtccgc
     6181 actaaaaagg aaatcgacaa agacgccatc ctgaatcaaa aagagcgttt ttcagacggc
     6241 caagtgccgg ggattaagat tgtgagcggc gtggaggatt ttgtgattga gccgagcgaa
     6301 caaattctat aaaatcaaca gcctgatttc aattcagtgt gttgaaacgt gattgagcct
     6361 acggagcagg agcttgccta gccggttcaa ataggcttta acccatgatt aaaggccgtc
     6421 tgaaatgggg tttaaaacct gtttcagacg gccttttttc ataccctaga ttcaggctgc
     6481 tttctcttcc tgctcgtaca cggcgttgta gaaagcggcg gacagcttgt cggctttctc
     6541 ggaggcggtt tcaatcacgg cagacaaacc gtcttcgatt ccttccatgt ccatatccaa
     6601 aactttcaga tggttgagcg tgaacaccag cagattcagg gctttgaggc tgtcttggtc
     6661 gaaagtcaga gtataggtgg tattcatggc ttaaccctcc aatcccaaag actgttgttg
     6721 caccaccatt ttgggcttgg ggacatattc caagaaaccc aaatcgttga gttttttgag
     6781 acggtaggaa accgcgccgg ggttcatgtc caagagtttg cctatttcgg tcaggttcaa
     6841 gcccatactg cggtagcgca gcagcgcgag catttcgggc gcggcttgga aataggcgtc
     6901 ttccaatgca tcgatgcggt atagcacggc atcaggcagg gcttttgcct gtttctccat
     6961 ttcgataaaa tatcggcggg cttggcgtcc tttgtcgttg cgctccacca tgcacagctc
     7021 ttttgccatg tcgagggata aataataccc ctgaacagtt ttatcacgct gtccaaagaa
     7081 acctgcttcg gtgcggacaa ttttgtccac gccgataaaa tcaagggctt gtgtaaatcc
     7141 gtattcttca atgcgacgct gaatccattt agaaaatggt gtttccacac ctaagaactt
     7201 atgcaggtca tgagcatcaa ccagtgcttg tgtttgattg tccaattgac ctgaaacagt
     7261 tgggatgagt tgggtattca tggtaaaatt atctttcatt tcaaggttga gatgacaagg
     7321 aaaagaacgc tctaacgttg cttttccact atccccgata agctgtcact tatcggggat
     7381 ttccatatct tgtgttgcac aagatatgga aattttaaat tatggtaatt ctatttgtca
     7441 atattctttt ttgctttctt tttgaatttt tccgattcca tatctcctct aattttcgat
     7501 tttacccagt ttgaaaaatc taaattttca gaaaatttaa ttaaatcaag ttctttttcg
     7561 gaattgaacg atactgtttt cactactcgc ttgagtgcgt atttttcttg agccttttta
     7621 atcgagtcca taatcttcct ttaaataggt tttaaaacac gattatggta atactaaaaa
     7681 ccattttaat caagatgtat tacaacaaaa agccgtctga aatggggttt aaaacctgtt
     7741 tcagacggcc ttttttcatg ccctacataa agagagttgc tgctctgctt tccacctgtt
     7801 ccgccgatgt tttcccatcg gcggaatttt cttatttcat tttttccttc atgactgatt
     7861 taacccagtt tgaaaagtcg ggaatttctt tttgcataaa atccaccaac tctttctctc
     7921 tttccaaatg gaaagaaact gttttcatca gccgcttttg gtcatacttt cgctgatatt
     7981 cagcttgttt tgcttttagc gttttttcat caggcatagg caatccttga tttttaaaat
     8041 cgttgtagta aaatggcttg cagaaaggtt ggcgaaaggg gcgcggtgtt ctgccacccc
     8101 ttgtgtttaa tcaacttgct tagtaagccg gactacagct taaaagcaga atgatgaaaa
     8161 aaacaacttt cgccagtttt gtcatttcca caagcctcct ttctatccga taagcccccg
     8221 aagttgccgc ttcggggctt tgctttatca gataggcgta ttatctagta tttctagaat
     8281 ttagtcaagc tattttttga ttttttcttt gatgattgat ttaacccatt gggaaaaatc
     8341 gatattttgg gcatattcca aaagttcttt ttccgtttcg gcattgaacg agactttctt
     8401 aatcacccgt ttctgctcgt aacgctttcg gttttctgcc agtttttcat caaccatagg
     8461 caaactcctt gattttttaa accgtatttt gtaagattgg gactggggac agcggcgacc
     8521 gcccccagct ggtcagtctt tagtaggcat tagcacttgc caacatgaaa actgcgacca
     8581 agatgagaat ttgaatagtg cgtttattca tcttctttct cccagttgag ccccgcttcg
     8641 gtggggcttt tcccgtatcg gccctcacta cgccgatgaa ttgaattata aacgtggaca
     8701 cgttaaaagt caaccctttc cccaacaaaa tcaaagaaaa atgccgtctg aaaacagctc
     8761 gaatgctgtt tcagacggca ttttttgttg tcgggcatca atgccctgcc atttgtgcgg
     8821 cgttgcggtt atcaagatat ggtattttag tgtgtatttt tagaagaaat ttaccatata
     8881 tagtattaaa atattatacg gttgttttat atggatttga ttttcttgaa aaataaatcc
     8941 catatgctaa tatttgcact atttgggcga ggttgaaaaa tgaataccgt caaagaatat
     9001 acggcagtcc gcgaacggct actcaatgct gccgattatt tggaagaagt ccgtaaagac
     9061 cgtaagacgg gcaatattgc gtccgtggaa tttgtgccgc cgaaaatagg ggctcggggt
     9121 tacggcaaat tcaaagtccg ttacaaaacc ttagtggcgg ttgatttatg agcggggaaa
     9181 aatccgagaa tctgccggcc gatgagcagc agaaaatgct tcaggagttt ttagaacttc
     9241 aaaaacagga tattgcagtc aaacagaagg agttggaaag ccgtaaggac gaaatccaat
     9301 ctcacgaacg cattgcactg gctaccatcg aagcgcaaaa acagggtgaa cagcaacatg
     9361 gtgagatttt caaagaggta cacaaacgcc gtctgaatgc cgtgattgct atttcttttc
     9421 tgattgccgc cgtgctgatt actgcgttat ggcgcgataa ggacagcgtc gctattgaaa
     9481 ttgtcaaaat cggcggggcg gtagctttgg gatattttgc aggaatcaac agaggtaaag
     9541 cgcaaacctt ggaaaagcaa cggcgcgaaa aagacagtga ttagattgta gtaaaaaagg
     9601 tcgtctgaaa cgttttggcg aaactcgcaa agctcgtttt cagacggc
//
LOCUS       DAWKOD010000066         9588 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000066 DAWKOD010000000
VERSION     DAWKOD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.066"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..252)
                     /locus_tag="WJK73_001201"
     CDS             complement(<1..252)
                     /locus_tag="WJK73_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557682.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQN"
     gene            complement(249..1304)
                     /locus_tag="WJK73_001202"
     CDS             complement(249..1304)
                     /locus_tag="WJK73_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7557683.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1410..1892)
                     /locus_tag="WJK73_001203"
     CDS             complement(1410..1892)
                     /locus_tag="WJK73_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7557684.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1939..3075)
                     /locus_tag="WJK73_001204"
     CDS             complement(1939..3075)
                     /locus_tag="WJK73_001204"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ7557685.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            complement(3072..3617)
                     /locus_tag="WJK73_001205"
     CDS             complement(3072..3617)
                     /locus_tag="WJK73_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ7557686.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3614..5089)
                     /locus_tag="WJK73_001206"
     CDS             complement(3614..5089)
                     /locus_tag="WJK73_001206"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ7557687.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5200..5565)
                     /locus_tag="WJK73_001207"
     CDS             complement(5200..5565)
                     /locus_tag="WJK73_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557688.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5586..7496)
                     /locus_tag="WJK73_001208"
     CDS             complement(5586..7496)
                     /locus_tag="WJK73_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7557689.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7515..8159)
                     /locus_tag="WJK73_001209"
     CDS             complement(7515..8159)
                     /locus_tag="WJK73_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7557690.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8323..9141)
                     /gene="opc"
                     /locus_tag="WJK73_001210"
                     /gene_synonym="opcA"
     CDS             complement(8323..9141)
                     /gene="opc"
                     /locus_tag="WJK73_001210"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ7557691.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2074 a         2089 c         2736 g         2689 t
ORIGIN      
        1 gttttgtttc tgacaggcaa aagcactgtt tatttggctg tcaaaaggat ggttaaggaa
       61 agttatgcgc ccctgaagcg ggccccagat aaggatggtt gcgccgacgg cttcagacgg
      121 cattttggcg gcggtgttgg gttttgtatc cggtttgccg ttgtgttttg tgatgatgat
      181 tttgggcgcg gtttttctgt tttgatgtgt gaaatgccgt ctgaaaggcg gttcagacgg
      241 catagcggtc atttttgtgc ggtcaggcgg tcgaatatgc cgccgtcggc gaagtaggtt
      301 ttcatgatgt tgtcccatcc gccgaatttt ttttcgggag agaaggtgtc taagtctggg
      361 aagtcggctt tgtgtcttgc caatacttcg gggttgcggg ggcgcaggta gagtgaggcg
      421 gcgagttctt gcgccggttc gctccaaagg tattcgagat aggcgcgggc ggttttttgc
      481 gtgccttttt tcgcgacgac gctgttgacg acggcgacgg ggctttcggc ggaaatggtg
      541 tagctcggat agacgatttc aaactgtcct tgggtcagtt ttttgctgac gtagttggct
      601 tcgttttcaa aagtgatgag tacgtcgccg atgttgcgtt gtgtgaaggt ggtggtggcg
      661 gcgcgtccgc cgttttcaaa aacgggggtg tttttgagga tggatgcgac gagtttttgg
      721 gcttcctgtt cgttgccgtt ggtggctttc agaccgtaac cgtatgcgcc gaggaaggcg
      781 tagcgtccgt tgcccgaggt tttgggattg gcgatgacga tgttaacgcc gtctttggca
      841 aggtcgttcc aatcgcggat ctgtttgggg ttgttttttc ggacaaggaa aaccatagtg
      901 ctggtgtagg gcgcggcgtg gtcggggagg gcttgttgcc agcctttttc taccagtcct
      961 tttttttcga gcaggtcgat gtcggaggat tggttcatgg ttacgacatc ggcttgaagg
     1021 ccgttggcta cggataatgc ctgtttgctg gagccgccgt gggactgttg gatgctgacg
     1081 gatgtgccgg ggtgttcgga ttggtatgtt ttgataaata aggggttgta ttctttgtaa
     1141 aaatcccgtg ccacatcgta tgaggcgttg agcagggtaa tgttttttcc gtcggattcg
     1201 gtattggccg gggcattttg tccggacgga tggtttgaat cggctgcggg gctgcaggcg
     1261 gtgagcaggg ctgcggtata gagtgccggt gcgtaggttt tcatatgctt gtcctgtcgg
     1321 ttggtagatg gggcaacttt atacggctgt ctgcgcttgt ggaaataatg tttgatttga
     1381 agattatcag ttttggttat aaggacggat cagaggtgtt tccgcatcag ttcgcatttg
     1441 attttgatgc tggggtcaag ctgcaatact gccgaaccga gcgattcgta gcggttgaga
     1501 aggtaaaacg ggacggagtt gagcgatgcg tagagtttca gaaagctcaa accggatttg
     1561 tgggcgatgg tttccgcctg atgaagcagg gcagtgccca gtccgaggtt gtggaacagg
     1621 gggtggacgt aaagtgcatc gagttgtgct tcttggcagt cgatttggaa aaatccctgt
     1681 atgttgcctt tgtattcggc aacccaaagt gctttgtcgg gatcggaaat ggtcggcagg
     1741 tagctttctg tgtttaacaa gccttcccat acttttaggg cgtgttcgtt gtagctgagg
     1801 atgcaggtgt attggacgga gtgcaggtgg actttgaaga tgtctttgca gtcttgcacg
     1861 gtggcggggc gtaacagtgt cagcaggttc atggcagtat gtcggcggct tcagacggca
     1921 tctgtgccgt tggtcggatt atagggactg atgcagtttt tttgcttctt gaaatgcggt
     1981 gtccgaatcg gtgcttaaaa cggtaaagtg tcccattttc cgccctttgt gcgcggtttt
     2041 tttgccgtaa aggtgcaggt gcgcgtccgg ccggctttgc aggggaaacc aatccggttc
     2101 gccgccgtct tcctgccaaa cgtcgcccaa aatattcgcc atacagcaag aactcagcaa
     2161 tttggtgtca gcaggtggca ggttgcacat caggcggacc tgttgctgga attggtctgc
     2221 cgcgcaggcg tcgacggtat ggtggccgga attgtgcgga cgcggcgcga tttcgttgac
     2281 gaccaattca tgcgtgtcgc cgacaacaaa catttctacc gccaatacgc cgacgtagtt
     2341 caattcatcg gccaaacgct gcgccatttg tcgcgcctgt tgctgaatgt cggcactcag
     2401 tcgggctggg acgatggagt aggcgaggat accgttttcg tgaatgtttt cggcaggatc
     2461 gaaagtttgc acgttgtcat tgttcagacg gcatacgata acggaaattt cgccgcgcaa
     2521 gtccaccatt ttttccaaaa cgcaatccac gccgcggtgt tcggcaaacg cggctttgag
     2581 ttcatccacc gttttgacgc ggatttggcc tttgccgtca tagcccaatg tagcggtttt
     2641 caggatgccg ggcagaaatt gtatgctttc ttcagtgatg tcttcggctt tgcaaattgc
     2701 ttgatacggc gcggtttgca ggcctgcttt gcgtatccat gccttttcct gaatgcggtt
     2761 ttgcgcgatg gcaacgcagt cgccgctggg ggaaacattg gtatgtttgg cgagaaaacg
     2821 catcgcatcg gcattgacgt tttcgaactc ggtcgtaaca gccgcacatt ttgccaattc
     2881 ttccaaagcg gtttggttgt caaacggcgc acacaaatgg cggtcggcaa attccgctgc
     2941 cggcgcattc gggttgggat cgagtacggt tactttgtag cccatggttt tggcagcaac
     3001 agtaaacatt ctgcctaatt gtccgccgcc aagaatgccg agcatggcgg gcggagaaag
     3061 agatatgttt ttcatgctga ctcttcaaat tgtacaagtt gatagctata actaattctt
     3121 gacggatgtc ttgtatcgct ggaattacca gtttcagaaa tacagaatac tttttccata
     3181 aatttttctg cttttagaaa ttccagtatt ctgttttttt catccttata agcactgcgg
     3241 tctgtacccc atgcaagaat aatcatatca gcatcttcca aacatccttt aaatttggaa
     3301 aaatcggttt gggtgtttgc cctaattcct gtttgctgtg tagagtaact ggaaaaaata
     3361 tttaacattt tgaagttggt aaaaccgtac atatccaaga aacgtgcaag ttgggtaagg
     3421 gttttgtcgc ttctttcatc atttgcttta cttggattaa ttcctatagc aacagctgag
     3481 aaatttttag gattttctgt gttgccgctc catctaaccg ttaggatttc tcgacttttt
     3541 tcattatctg tatagagacc gtctttggta gtcggtctga gtgtttggcg aagctcataa
     3601 agtttttcat aagtcattta tccaaccctt cctgcaccat ctgcgcggcg cgtataacgg
     3661 ctcgggcttt gttttgtgtt tcctgccatt cggaggccgg atcggagtcg gcaaccacgc
     3721 ccgcgccgct ttggacgtat agcgtgttgt tttttactac ggcggtgcgg atggcgattg
     3781 ccaaatccat gtcgttgttg aaaccccata cgccgacggc accgccgtag atgccgcgtt
     3841 tgctcggttc gacttcttcg atgatttcca tggcgcggac tttgggtgcg ccggagagtg
     3901 tgccggcagg gaaggtagcg gcgaggatgt ccatgttggt catgccgtct ttcagacggc
     3961 cttcgacgtt ggaaacgatg tgcattacat gggagtattt ttcaatcacc attttgtcgg
     4021 taactttgac ttcgccggtt ttactgatgc ggccgacgtc gttgcgtcct aagtcaatca
     4081 acatgacgtg ttcggcgatt tctttggcat cgcttaacaa atcttgttcg ttggcaaggt
     4141 cttcggcggg ggttttgccg cgcaggcgcg tgccggcgat ggggcggacg atgacatcgt
     4201 cgcgttcgcg gcggacgagg atttcgggcg aggagccgac gatgtggaaa tcgccgaaat
     4261 cgtagtaaaa gagataaggc gaagggttga gcgtacgcag ggcgcggtag agggcgagcg
     4321 ggctgtcggt gaattccatg ctcatgcgct ggctggggac aacctgcatg cagtcgcctg
     4381 cgaagatgta gtctttgatt ttgttaacgc aggctttgaa cggctcttcg ccgaattcgc
     4441 tgacggcttc ggtgtgtttg ctgccgagcg agagcgggat ggcgcagctt tggcgcaact
     4501 gggtgcggat gtcttcgagg cgttcgcggg cgcgttcgta gccgtcgggc tgcgacggat
     4561 cggcgtaaac gacgagatgg attttgccgc tcaaattgtc gatcaccgcc aattcttgcg
     4621 acagcatcag caagatgtcg ggcgtgccga gcgggtcggc tttggtggtg tttttcaggc
     4681 ggtgggcgaa gtgttcaaaa ttgtagatgg tttcgtaacc gaagtagccg accagtccgc
     4741 cggtaaagcg cggcaggctt gggatttcgg gtgttttgaa gcggttgtgg aaggcttcga
     4801 taaagggcag ggggttgccg tcgtgttgct cgacaatttc gccgttttga taaacatcga
     4861 cgtgtttgcc gctggctttg agatagtggc tgcaaggcag gccgataaaa gaatagcggc
     4921 cgaaacgttc gccaccgaca acggattcga gcaggtaggt ataggggcgg ttggcgagtt
     4981 tgagatagag ggaaagcggc gtatccaaat cggcaaggag ttcttgcacg agcgggatgc
     5041 ggttgtagcc ttgggcggct tgggcttggt attcttgttt gctgatcatt tctgctttcc
     5101 caaagggcgg tttcggacgg cgcggcaacg ggcgcgagta tagcatttta tcggaattgt
     5161 tgacagtctg accggagatg ccgtctgaaa aggacgggct tacggctttg ttttgcgctg
     5221 ccggtattgt ttccatttaa acagataacc tgcggttacg ggaatcaggg ctaagatggt
     5281 tttgaacgtc catgatgcat accaaggtaa agaaccgggt gcgaccgatt gattcagata
     5341 cggccaccaa caggcagtca ggcagccgag cagcagcggc cagatatgcc ggctgaggtt
     5401 tttttgccaa aggaggaatg atgcggcaga ccaaagcagg gtaagggcag tgatttttaa
     5461 cacggtataa gcatccgctc caaacagggc ggcgagaaaa tacgtaccgg caatccagag
     5521 gacggcaagt atcaggacaa tgatttcttc aggtcgtttg aacatttttt tcttcctgtt
     5581 tgatttcaga cggcattgcc gttctgtttg gtttccagca gctcccagcg ttccagcttt
     5641 tccaaaagca gcatttcgat ttcttcggcg cggctttgca gtgcgccggc tttttcgtaa
     5701 tctttgaaga tttcgggatc ggaaagctgc gcgttgattt ctgcctgttc ggcttccaag
     5761 gcggcgattt cgtcgggcag ggcgtcgagt tcgcgctgtt ctttgtagga cagtttgacc
     5821 gtgcggttgg ctttgggttt ttctttcacc ggttcggcat cggctgtttt gggtgcggat
     5881 tccgtctgaa tcttcgcttc gcgtgatttt gcgtcgatgt agtcctgata gccgccgata
     5941 tattctttta accttccatt tccttcaaaa ataatgcttt gggtaatcac gttatcgagg
     6001 aacatacggt cgtgtgagac aaggaatacc gtgccttgat aatcgcgcag caaatcttca
     6061 agcagctctt gggtatcgat atccaagtcg ttggtcggtt cgtccaaaat caaaatatta
     6121 gcaggacggg taaagagttt tgccagcaaa aggcggttgc gttcgccgcc ggagagcgat
     6181 gaaacgggac tttgtgcgcg ggctggatgg aataagaaat cttccagata gctcattacg
     6241 tgttttttct tgccgccgac ttcgacgtaa tcgttgcctt gaccgagcgt gtaaaacacg
     6301 gtgtcgtttt cgttcaacgt gctgcggaat tggtcgaaat aggcaacttc ctgcttactg
     6361 ccgatacgga ttctgccgta ggtcggctgc aattcgccca aaatcagctt gaggaaggtg
     6421 gttttgccga taccgtttgg gccgattaag ccgattttgt cgccacgctg caagatggcg
     6481 gagaatttgt ccatgatgac tttgtcgcca taggtaaacg aggcatgttc cagttcggca
     6541 atgattttgc cgcttttctc gccgctgtcg agcttgaaat tgacctgccc ttgtacgttg
     6601 cggcgttcgg cacgctggcg gcgcagttct tccaaacggc gcacgcggcc ttcgttgcgg
     6661 gtacggcgcg cttcgatgcc tttgcgtatc cacgcttctt cctgcgcgtg gaatttgtcg
     6721 aagaggcggt tgtgttccgc ttcgactgcc aactcttgcg cttttttctc gctatatttg
     6781 gagaacgagc cgggatagga acgcaaaata ccgcgatcga gttcgacaat ccgcgtggcg
     6841 atattgtcca aaaaacggcg gtcgtgggta atcacaacca ggctgccttc aaacgctttg
     6901 agcaggtttt ccaaccaaat aatcgcgtcg atgtccaaat ggttggtcgg ttcatcgagc
     6961 agcaatacgt cgggcttctg cacccaagcc tgcgccaagg cgacgcgctt tttctgaccg
     7021 ccggagaggt tgccgatttt ttcgttttcc ggcaaaccga gttcgcccaa agtctgcttc
     7081 accgccgcat ccaacttcca gccgtccttc gcttcgatct caagttgcaa ttcgttgagc
     7141 tctttcaata aggcctcact cgaaccattt tccaactcat ggctgacatg atgataacgg
     7201 cgcaataaat cgcgaatttc gcccaaacct tcggcaacag tatcaaatac ggttgcttcc
     7261 ttatcaaaaa aggattcctg cggtacataa acgattttga ggttgttttg gacaataatc
     7321 tgcccatcat caagcttttg cacgccggcg aggattttta aaaacgaaga cttacccgca
     7381 ccattacgac cgattaagcc gattttttcg cctctgtcga gttggaagga agttttgtcg
     7441 agcagggcga cgtggccgac ggcaaaggaa gcgttttcta cagataatat attcatgata
     7501 caaattctca acagttaccg tttggatttt accgcaagtt tggcgcgggc aatttcaacc
     7561 gcacccggca ggacggaaac aataatgatg ccgcccatca ccaagcccag attgtttttt
     7621 acgacgggga agttggcaaa gaaatagccc gcgtaagaaa acaggataac ccacaacaag
     7681 ccaccgatga tgttgtagcg gataaatttg gcatagtgca ttttccccat accggcgacg
     7741 aagggggcga aggtgcggac gatgggcata aaacgggcaa tgatgatggt tttgccgccg
     7801 tgtttttcgt aaaaacggtg ggttttatcg agatattcac gtcggaagat tttagaatcg
     7861 ggattggcga acagcctgcc gccgaaatat ttgccgacgg taaaattgag cgcgtcgccg
     7921 agtatggcgg caaggcttaa taatgcaacc atcaaatgaa tatccatacc gcccagcgcg
     7981 gcaataccgc cggcggcaaa cagcagcgaa tcgccgggca gtaagggcgt aacaatcagg
     8041 ccggtttcgc aaaaaacaat caaaaacaga atcgcataaa tccacacacc gtattgcgcc
     8101 gacagcgcga gcaggtgttg gtcgatatgg aggatgaagt cgatggcgga ggcaagcacg
     8161 gcgcgttcct aaaaaaaaca aaccgcgtat tttaaccgat tggaaaaatg ccgtctgaaa
     8221 ttttcagacg gcatcggcta ttcaaattca tttcacgtaa aaaccgcaaa ccaaaatagt
     8281 ttgcggtttg gcatttaaag tgacaatgat gatttcaaat catcagaatt ttatgccgac
     8341 gcgcaagccg tattcacgaa tactggtttt cgggatggtg agcgatacgt cgccactctt
     8401 ggttgttaca ctaaactcgc cggattcttt gtaagtgcgt tgtttgtaga acggccccgc
     8461 ctcgatgctg gcggattcgc ccagtttttt accaatattt gcacccaatc caaagcccca
     8521 gccgttggtt ttttgattga tgtctttttt gagattggta acgccggtgt ttaatttata
     8581 gcgggaattg aggtcaaatt tcacttcaga ccaagggttg atataccagc cgttacccag
     8641 ttgggaaagc aaatccgcgt gaactttggc taaccacgac tgacggctgc tgtgaagcgt
     8701 atgcttggtg gttttaatgc tgtcttttga agattcaaaa cccaagccgg cacccacacg
     8761 gaaatttaaa gaatcactta acgtttgggt gtaggtgtag cctgtgtaaa gatcgatacg
     8821 gttttcagga acgccggtgg gcagttttac atttttagtc ttacccagct tgttctcatc
     8881 tgtttccaaa ttaataatat tttttttgct gcgcccgaaa ccggcttcca agcggatgcc
     8941 ttggttggca tcaaaaggaa tatcagcacg tacgccgatg tgtttggcag ctttgtgttt
     9001 ttctttcagg aaagcacgag ttgaagaaat ggaagagagg tcggtgtgga cggtaaactc
     9061 attagcggtt tgaagctctt gtgcagcggc ggcagtaccg gtcagggcaa tcatggcaca
     9121 tgtaaaaact gtttttttca tagttaaaat ctccaaaatt tggattgtag tcggatatgg
     9181 taacataacg taaataatcg ttacgcttac aattatattc ttaagctttc gggggggggg
     9241 gggattttac atatattaat aaaaattaac aaatagttat ttgtttacaa cgaattgtta
     9301 ttctcacttg gttttctgtt ttttatggga atgacgaaat tttaggtttc tgatttcggt
     9361 tttctgtttt aagggaatga cgagacttga gatggcggca tttatcggga gcaactgaaa
     9421 ccactctgcc gtcattcccg cgaaagcggg aatctaggtt cgtccggttt cggttatttc
     9481 cgatagattc ctgccgcgtt gggggtctgg attcccgcct gcgcgggaat gacgaatttc
     9541 aggttgctgt ttttggtttt ctgtttttgt gaaaataaca ggatgaga
//
LOCUS       DAWKOD010000067         9465 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000067 DAWKOD010000000
VERSION     DAWKOD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.067"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WJK73_001211"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WJK73_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ7557692.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WJK73_001212"
     CDS             1045..1524
                     /locus_tag="WJK73_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557693.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WJK73_001213"
     CDS             1526..2524
                     /locus_tag="WJK73_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ7557694.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WJK73_001214"
     CDS             2645..3064
                     /locus_tag="WJK73_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557695.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WJK73_001215"
     CDS             3140..3655
                     /locus_tag="WJK73_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7557696.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WJK73_001216"
     CDS             complement(3739..4623)
                     /locus_tag="WJK73_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ7557697.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGSIDNDRLQQEFALFVQKS
                     DVDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WJK73_001217"
     CDS             complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WJK73_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ7557698.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WJK73_001218"
     CDS             complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WJK73_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ7557699.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7841..8209)
                     /locus_tag="WJK73_001219"
     CDS             complement(7841..8209)
                     /locus_tag="WJK73_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ7557700.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8109..8567)
                     /locus_tag="WJK73_001220"
                     /pseudo
     CDS             complement(8109..8567)
                     /locus_tag="WJK73_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8763..9044
                     /locus_tag="WJK73_001221"
     CDS             8763..9044
                     /locus_tag="WJK73_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557701.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDRA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYISWLGLAEPCRFRR"
     gene            9044..9436
                     /locus_tag="WJK73_001222"
     CDS             9044..9436
                     /locus_tag="WJK73_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEZ7557702.1"
                     /translation="MMPPVTGVPNSHLLFDVAFAVVVLSLLIRGMTIPVMARLLKVAM
                     PNKPEPKDTHDIWLAKKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFGARCFALIRN
                     GIRIEMQSDTVLQAGIWRGTSCPTARSI"
BASE COUNT         2082 a         2345 c         2788 g         2250 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aaggacatca
       61 ttcctgcacc ggcaagaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 ctcgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gtaccgtcgt cgtcaatgct
      481 gccgaagctt tgggaggcac acccgatgcg gacgcttccg tagacgacac gggcaaagtt
      541 gcctgtgctg tcatgactgc cgactgtctg cccgttctat tttgcgacag ggcgggtacg
      601 gcggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgccgcga tgaaggttcc acccgtcgaa atgatggcgt atctcggtcc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggatgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtat atggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagttatcaa cgaatatctt
     1321 ttaatcttga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacacattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgcc ttcgattgga acgaactgtt gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc caacggaaaa cccggcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttgctgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcgc gtcaggaaat cgacaagctc gggctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagtgtcc aatccgtacg
     2341 caacagtctc agactttggg gtgaaaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgcgcc caaatcgacc gaatcatcaa
     2461 aggtgcggaa gacggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ctaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ctcctggccg cttgccatcc tgcttgccgc
     2761 cgtcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgcacctg ctgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggtcggtgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg atcggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggcggca atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgttttccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgcaggcggc tgaattcttc
     3961 atcgacatcg gatttttgga caaacagggc aaactcttgt tgcaggcggt cgttgtcgat
     4021 gctgccgacg gcttcggcaa ggcgggcgcg gattttttct ttatgcgctt ccactagcgc
     4081 ggggaagatt tcgctcaatg ccccgatgat ttcttccata gactcgaggc gttgcagcag
     4141 gtgttcgccc aattttttcc cttcgcgtcg gcgtgcggcg gtaaagtctt tcagtgccga
     4201 atcggtcaaa tcggcaataa cctttgccaa tgcgtccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt ccaaagactg
     4381 attgccgttt tccgtatctt gaatttggat tttgcattcg actttgccgc gtgccagatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatggcgc aaatcatccg gcatcctgat
     4501 ttgaatgtcc aaaaagcggt ggttgacggc gcggatgtcg aggttgatgc gtttgctgcc
     4561 gcattccgcc gccgcgttgg caaagccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaaccaaa accagtacgg cgttgcctcg
     4801 ccttagctca aagagaacga ttctctaagg tgttgaagca ccaagtgaat cggttccgta
     4861 cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatca
     4921 attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat gtctgctttt
     4981 tgatttcggc gattgcgatg gtttggacgg ataaaatcgt gtacggcacg acgcacaaac
     5041 cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta ttttattgcg
     5101 tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct gcatggcttt
     5161 tgcttcgatt tggcggatgc gttcggcaga tacgccgtat tcggcggcaa gctggtgcag
     5221 cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc tcctgtcatc
     5281 cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg cctgtttcga
     5341 tagttgccgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg cgaaactgtc
     5401 ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc gctgttccat
     5461 ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg cctctttggg
     5521 agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct tgcgttgcgg
     5581 tttggtggtg gcaacgcgaa ccaaacgcca gtttctcaaa ataaactcgt ggatttcggc
     5641 tttaatccag tgtacggcaa atgaaaacag acgcgcgcct ctaccgggct cgtagcgttt
     5701 gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat tcagcccgta
     5761 gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga tgagttgttt
     5821 ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt cttcctggga
     5881 cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt tgccgctttg
     5941 gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc tgaaactgcg
     6001 tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta ttttgtatgt
     6061 ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg caagtttgat
     6121 tgattttgta aacctgatgg ttcaattcta tttcagaatt atattacata cgtagttgta
     6181 tgtaaacggc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag gtatcagtgt
     6241 tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc catcaaccac
     6301 catgaactgc cggttttcat atagggcgtt tcgccgacat agcctttgat gtgtccttcc
     6361 aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg ggagatgatg
     6421 gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat agcccgcgcc
     6481 tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc catattgctg
     6541 gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc gaatccgtct
     6601 tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga ttgcttaccg
     6661 ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg cgtggtcagg
     6721 aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta gtaggggatg
     6781 ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc gtaaccgttg
     6841 ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt ttgcgcctgt
     6901 tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg gatggcggtt
     6961 tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata tttctgtatg
     7021 gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc ttgaagcagg
     7081 gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc ggtgtagcct
     7141 gcggcaagca gggcggcaat caggataatt ggaagcaggc gtttgcccga acgtgcggtg
     7201 ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt aaccatgtgg
     7261 atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg ggagtagccg
     7321 attgcgcccc agccgaatcc ggtcaggaaa cgttcgcggg caaactcggt cagcgtccac
     7381 aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt tttccacagc
     7441 cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt cagcggtacg
     7501 gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca gtagaactgt
     7561 gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg cggacgcagt
     7621 tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg gtagtagggt
     7681 gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta gagggcgggg
     7741 tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg aagataccgt
     7801 ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg ccgaaacgat
     7861 tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg acaacgaggt
     7921 tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa tcgtgcagaa
     7981 ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc gtgccgacca
     8041 cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc aaactgcgcg
     8101 aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg acgatgacgg
     8161 caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt acgagcatgc
     8221 gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa gcacagataa
     8281 atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga aagcagcagg
     8341 ggcgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga ccaaacaatc
     8401 ggcaactcgc gttctgccga tacgcccgta acccggttgg cattgggtgt caaatcgtcg
     8461 ccgaccatgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac atcgtccaaa
     8521 acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa agaatcctga
     8581 aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt ttcaggcggc
     8641 atgaaacagg aaaatgccgt ctgaacgctg tggcggacgt gaagtaaagt ttcgtgaaaa
     8701 gaaaataccg tgttacagtc tttcgatttt aatttcatga attttaaggg agaatcgtta
     8761 gcgtggattg gatgggcagt ctgttcctgc cgggtggcgc actgttgttt ctgagcgtgg
     8821 tttcgaccac tttgtccgca cgtttgggaa tgcctttgct gctggtttct cctgccaacg
     8881 tgttggacag ggcggcggaa gccttggcga ttgcggcgtt cctgatgctg gtcgcgcgtc
     8941 cgccggcagt gttcggcggt ttgtggaaat tcaattacag cctgcgtgaa aaggcgtata
     9001 tcagttggtt gggtttggcg gagccgtgcc gatttcgccg gtgatgatgc cgccggtaac
     9061 gggcgttccc aattcgcacc tgctgtttga tgtggcgttt gccgtggtgg tgctgtcgct
     9121 gctgattcgg ggaatgacga ttcctgtgat ggcgcggctg ttgaaggttg ccatgcccaa
     9181 caagcccgaa ccgaaagata cgcatgatat ttggctggcg aaaaaggaaa ttgtcagaat
     9241 gtcggcgttt aaagtggttg ccgaatctga agcggaggga caccatcccg atacggtcga
     9301 accgatttcc gattcgtttg gcgcacgctg tttcgcattg atacgcaacg gcatccgaat
     9361 agaaatgcag tccgacaccg tgcttcaggc ggggatttgg cgtggtacat cctgcccgac
     9421 ggcaaggtcg atatagtgga ttaacaaaaa tcaggacaag gcggc
//
LOCUS       DAWKOD010000068         9431 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000068 DAWKOD010000000
VERSION     DAWKOD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.068"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WJK73_001223"
     CDS             complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WJK73_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ7557703.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1615..1815)
                     /locus_tag="WJK73_001224"
     CDS             complement(1615..1815)
                     /locus_tag="WJK73_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557704.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1824..2213)
                     /locus_tag="WJK73_001225"
     CDS             complement(1824..2213)
                     /locus_tag="WJK73_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557705.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2422..3339)
                     /locus_tag="WJK73_001226"
     CDS             complement(2422..3339)
                     /locus_tag="WJK73_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7557706.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFVGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSVGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3511..4311
                     /locus_tag="WJK73_001227"
     CDS             3511..4311
                     /locus_tag="WJK73_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557707.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4359..5135
                     /gene="mlaE"
                     /locus_tag="WJK73_001228"
     CDS             4359..5135
                     /gene="mlaE"
                     /locus_tag="WJK73_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ7557708.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAIDFVLTAWMFTD"
     gene            5187..5681
                     /gene="mlaD"
                     /locus_tag="WJK73_001229"
     CDS             5187..5681
                     /gene="mlaD"
                     /locus_tag="WJK73_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ7557709.1"
                     /translation="MKKNILEFWVGLFVLLGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5718..6308
                     /locus_tag="WJK73_001230"
     CDS             5718..6308
                     /locus_tag="WJK73_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ7557710.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTTRQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6365..6643
                     /locus_tag="WJK73_001231"
     CDS             6365..6643
                     /locus_tag="WJK73_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ7557711.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTGDAFERFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6640..7467
                     /locus_tag="WJK73_001232"
     CDS             6640..7467
                     /locus_tag="WJK73_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ7557712.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7592..7954
                     /locus_tag="WJK73_001233"
     CDS             7592..7954
                     /locus_tag="WJK73_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ7557713.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7951..8337
                     /locus_tag="WJK73_001234"
     CDS             7951..8337
                     /locus_tag="WJK73_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7557714.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8334..8822
                     /locus_tag="WJK73_001235"
     CDS             8334..8822
                     /locus_tag="WJK73_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7557715.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8904..9071)
                     /locus_tag="WJK73_001236"
     CDS             complement(8904..9071)
                     /locus_tag="WJK73_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ7557716.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9038..9412)
                     /locus_tag="WJK73_001237"
     CDS             complement(9038..9412)
                     /locus_tag="WJK73_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ7557717.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2182 a         2643 c         2365 g         2241 t
ORIGIN      
        1 cgtttaaatt taatccgcta tacaaagaca aaaacatcga cctcggtcat gactgattgc
       61 cgatgaagca ataaaaatgc cgtctgaacc tgaaaacggg ttcagacggc attttctatc
      121 ggggttttaa agcggcatta aatgtcggtt tccaaataaa cgacttgggt ttgcagatat
      181 tcttccaaac cgtgtttgcc gtccgcgccg ccgataccgg attttttcca accggcgtgg
      241 aaaccctgca tcgcttcaaa gttttcgcgg ttgatgtagg tttcgccgaa ttgcaggcgg
      301 cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa cagaactggt cagaccaaac
      361 tcgcaatcgt tagccagtgc gatgacctgg tcgagcgtgt cgaaagcgga aacgggcagc
      421 acggggccga aggtttcttc tttcataatg tccatactgt tgtcggtgtc ggtcagcagg
      481 gtcggctcga agaaataacc gcgtccttcg gcgcgtttgc cgccgcaaac caatttcgca
      541 ccttgtttga ctgcccgttc cactttttcg gcaacggctt tgacggcgcg ctcttcaatc
      601 agcgggccca tttccagcgc gcctgcttcg gcttcggcag ggttgccgta gcgtacgcct
      661 ttcatggcgg cggtcatttt ttcaatgaat gcgtctttca gactgctgtg gacataaacg
      721 cgctcggcgc agttgcagat ttgaccagtg ttgccgacgc gcgaagccaa gatggatttc
      781 actgccaagt ccaaatccgc gtctttcaaa acgatagcag gtgctttgcc gccgagttcc
      841 agcgagactt tggtgatgtt ggcagaagcg gcttccatca cttggcggcc cgcttcaacg
      901 gagccggtca ggctgaccat atcgacttgc ggatgggcgg acaaggcatt gccgatttcc
      961 gcacccggac cgttcacaac gttgaacacg cctgctggca gtccgaccgc atcgacgatt
     1021 tcggcgaaga tgtggcagtt gatcggggtt acgctgctgg gtttgacgac gatggtgttg
     1081 cccgttacca aagcggggcc cattttgcgg gcaatcagga agaaggggaa gttccacggc
     1141 aaaatgccgg cgattacgcc cagcggacgt ttgaacaata aaatattttc gcgcgggcgg
     1201 tcgctttgga tgatttcgcc ttcgtaacga cgcgcccatt cggcttggta atcgagatag
     1261 tcggcggtaa acatgacttc cacgcgcgcc aagtctttgg ttttgccgcc ttcggcaacg
     1321 atggtgtcgg tcagctcgtc ggcgcgtccg cgtatgcctt gggcgatttt gcgcaaatac
     1381 gcgccgcgtt cgaccgcagg caggcgttcc caagccggtt gcgccgcgcg tgccgccgcc
     1441 acagctcggt caacgtctgc ttttccgcct ttgggttcgc gggcgatggt ttcttcggtg
     1501 gatgggttca atacgtcgcg ccattcgccg ttgaaatcgt tttcaaagcg tccgttgatg
     1561 tacatggcca attgtttcat ttcgggttct ccagttttgt agtcagatgt agtcttagtt
     1621 tattcccaaa taaattgccg tacaagtttc ttttgaaatg tttagacgag aagctactat
     1681 gtctgcatat tgttctgcta atttatattc actcgaatgc aactgccgat tattccaatc
     1741 tgcattcgca tcagtaaaag ccccagcatt gccgcctgtg gcataaccaa tcgctaaact
     1801 gccaacggta tccacaacag gtttcaggct gcctgaattt ctctaataaa tgaaacactt
     1861 attctatcag ccgttaatgt aaccaaacaa ttatcaagat aagagtagtt tactaaatac
     1921 tcatcttctt caaattgtat attttgtagg gttagatgat tattttttaa ttctgccgtt
     1981 atttcatact tgaaactatc atctattttt ctaaatccaa tatttgaagt tgatttatcc
     2041 agtatgtaat cattaaagat ttcataggat aattccactt cataaaattg attttcaaat
     2101 attttgaatg ggcagtatga tataaaacaa tgtagttcat aaccatttat ttcaatcaat
     2161 actgcctctt caatggtgtt atctaacttt ttgattttac atttatataa cattttatct
     2221 tgcctttaga taaattgttt tgttttttgg attgttattc aaagttctcc aaaagtcttt
     2281 tcctgatttt gtatgtatcg tggtaacact accattttca ttggttccaa caaaaataaa
     2341 tttagattga cctttggaat cagtacccaa aaactgaaca tatccagaca tgcggatttc
     2401 gggttttaaa tcaaatgcgg cttaccggtt ttcttttttg gtttccgccg gcgtgctgcc
     2461 gaaatattgc ctgaatgcct gagtaaagct ggaaacgtgg cggtagccgc ataaatacgc
     2521 ggtttcgcct acgcttttgc ccccgttttg caacagatag agcgcgtgct gcatttgttt
     2581 gtggtgcagc cactcgcttg ccgtaatgcc gaaatggtcg cgcatacggc gttgcagcgt
     2641 cctttcgctg atgttcagcg cgtctgtcag ccggttgact tggtgtgcgc cgccgacaaa
     2701 cgcggcattc agggtgcggc tgaagtcttc agacggcata gcgtctgctt ccgccgtttg
     2761 ccccgccgcc ggcccgatgc cgtctgaaac cgtgtcccac aagtccgaca gcagccgcaa
     2821 aacgtccgcc tcgcggcgca atgtttcgcc caaatgcccc tttgggacgg ctttcaggca
     2881 ggatgccgcc aagccgcgca ggttcggggg caaatcccat atcctgaccg gttcgcggta
     2941 aagcaggggt gcgaaacgcg cgtattccgg acgcagcagc cattgttcca tacctttaat
     3001 ggtcattttg accgttttgc cgcctcggta aagatagcgg ctgaacagga cttcttcccc
     3061 gacagcaatt aggacaatct tgccgccgtc cgcatcgatt tggaagcggc agcggttgat
     3121 gccgaagtcc aaactgcctt ccagcaagag cacgaacgac acataaggtt cggcgaggcg
     3181 gctgctgcaa aaatcacacc gtgccgttac cgtgccgccg tggatggaaa tgccgttgga
     3241 caaagtatcg aaacggtagc tgccctccac atagctgccg ccgccgcttt tgaccagatt
     3301 gacgaaccgg ctgctgttca aatgttctgc tttttccata gttttctgat aaaggttatc
     3361 atttgaaaga taacattttt cgccacagca aacaatccgc cgccacaacg gcattttcac
     3421 aacacttgcc ggccgtatgc cgtctgaaaa ttgcccgtct tcccgataag ccttaaaata
     3481 cacgccgtca aacctaccgt ccgtccgtac atgagcccat ccccctttat cgaaatgaaa
     3541 gacgtcgctt ttgcgtatgg cgaccgcccg attctgaacg acatcaattt cagcattccg
     3601 caaggcaatt ttgccgccgt gatgggcggt tcgggcagcg gcaaaaccac gctgatgcgg
     3661 ctgattacag ggcagattcg tccgcagtcc gggcaggttt tgattgaagg gcgggatttg
     3721 tcgggctttt cggctgacga actctacgaa caccgccgcc gtatgggcgt attgttccaa
     3781 cacggcgcgc tgtttactga tttgtctgta tttgacaata tcgcttttcc gatgcgcgaa
     3841 ctgacgcagc tgccggaagc ggtgattcga gatttggttt tgttgaaact gaacgcggtc
     3901 ggtctgcgcg gtgtagaaaa cctgatgccg tccgagttgt ccggcgggat gtcgcgccgc
     3961 gtcgcgcttg cccgcacgat tgcgctcgac cccgaaatta tgttgtacga cgaaccgttt
     4021 accggtctcg atccgatttc cttgggcgtg attgcccact taatcagccg cgtcaacaag
     4081 gctttgcgtt cgaccagtat tatggtaacg cacgacattg aaaaatcttt ggaaatcgtc
     4141 gatcaggtga ttttcttggc gcacggcgaa attatgttct ccggctcgcc gcaggaaatg
     4201 cgcgaactgg attcgccttg ggtgcgccag tttgtcggcg gcttggcaga cggtcccgta
     4261 gcataccgct atccggcgca aacgtcgttg cagcaggacc tgttgggata aatgagttga
     4321 tgccgtctga aaccgttgaa ctttacacat agaaatttat gaactttatc cgttccgtcg
     4381 gggcgaaaac cctcggcctt attcaatccc tcggcagtat cacgctgttt ctgctgaaca
     4441 ttctggcgaa gtccggtacg gctttcgtcc gtccgcgcct gagcgtgcgc caagtgtatt
     4501 tcgccggcgt gctgtcggtg ttgattgttg ccgtttcggg gctgtttgtc ggtatggtct
     4561 tgggtttgca gggctatacg cagttgtcga aattcaaatc cgccgatatt ttgggctata
     4621 tggtcgcggc ttcgctgttg cgggaactgg gtccggtgtt ggcggcgatc ctgtttgcca
     4681 gcagcgcggg cggtgcgatg accagcgaaa tcggtctgat gaaaacgacc gaacagctcg
     4741 aagcgatgaa cgtgatggcg gtaaaccctg tcgcccgcgt ggttgcgccg cgcttttggg
     4801 cgggcgtgtt ttccatgccg cttttggctt cgattttcaa cgtggcgggt attttcggcg
     4861 cgtatttggt cggtgtaacc tggctgggct tggacagcgg cattttctgg tcgcaaatgc
     4921 agaacaacat cacgatacac tacgatgtaa tcaacggtct gatcaagtcc gccgcgttcg
     4981 gcgtggcggt aacgctgatt gccgtgcatc agggcttcca ttgcgtcccg acctcggaag
     5041 gcattttgcg cgccagcacg cgtacggtgg tttcgtccgc cctgacgatt ttggcgatcg
     5101 actttgtgtt gaccgcgtgg atgtttacag attgacaggc cgtctgaaaa cgaaacaaaa
     5161 aacatttgga tattcaagga actttaatga aaaagaacat attggaattt tgggtcggac
     5221 tgtttgtcct gctcggcgcg gcggcggttg cctttctcgc tttccgcgtg gccggcggtg
     5281 cggcgttcgg cggttcggac aaaacttacg ccgtttatgc cgatttcggc gacatcggcg
     5341 gtttgaaggt caatgccccc gtcaaatctg caggcgtatt ggtcgggcgc gtcggcgcta
     5401 tcggacttga cccgaaatcc tatcaggcga gggtgcgcct cgatttggac ggcaagtatc
     5461 agttcagcag cgacgtttcc gcgcaaatcc tgacttcggg acttttgggc gagcagtaca
     5521 tcgggctgca gcagggcggc gacacggaaa accttgctgc cggcgacacc atctccgtaa
     5581 ccagttctgc aatggttctg gaaaacctta tcggcaaatt catgacgagt tttgccgaga
     5641 aaaatgccga cggcggcaat gcggaaaaag ccgccgaata aacgtatgcc atcatattca
     5701 aactaggaca cttaatcatg aaaaaatcct ccttcatcag cgcattgggc atcggtattt
     5761 tgagcatcgg catggcattt gccgcccctg ccgacgcggt aaaccaaatc cgtcaaaacg
     5821 ccactcaagt attgagcatc ttaaaaagcg gtgatgccaa caccacccgc caaaaagccg
     5881 aagcctatgc gattccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc
     5941 cttggcgcac cgcgtccgac gcgcaaaaac aagcgttggc caaagaattt caaaccctgc
     6001 tgatccgcac ctattccggc acgatgctga aattaaaaaa cgccaacgtc aacgtcaaag
     6061 acaatcccat cgtcaataaa ggcggcaaag aaatcatcgt ccgcgccgaa gtcggcgtac
     6121 ccgggcaaaa acccgtcaac atggacttca ccacctacca aagcggcggt aaataccgta
     6181 cctacaacgt cgccatcgaa ggcgcgagcc tggttaccgt gtaccgcaac caattcggcg
     6241 aaatcatcaa agcgaaaggc gtggacggac tgattgccga gttgaaggct aaaaacggca
     6301 gcaagtaatc cataagccat gccgccagtc tgaaggcggc atggattttt tcctaccgaa
     6361 cactatgcat acagaactca acaacggaac actgcacatc agcggcgacg taaccgtcaa
     6421 aaccctgacc ggggacgcat ttgagcgttt ccggcagcaa tgccgtctga aagaaaccat
     6481 tgccgtcgat ttcggcggtg tcaaacgtgc cgattccgcc tgcgtgtcgc tgctgctcga
     6541 agtgctgcgc ggctgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc
     6601 gctgtccgaa ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc
     6661 ttcctcctgc tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac
     6721 ccttatgaag gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt
     6781 ttcgcccctg ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg
     6841 tccaattttt ttaataacct gtgcgacgtg gtcagcttcg gcagcaatat cttgcgcttg
     6901 gacatcaaac gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg
     6961 ggcgggctta tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc
     7021 gacacgtttg cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg
     7081 ccgtccaccg tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat
     7141 atcgtcttcc gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg
     7201 cgcgaaggcc tgctcgattt gaccgacagc ctggacgaag ccgccatcga caaatacagc
     7261 tacacgcgcg acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa
     7321 ggtacggaag ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg
     7381 gaaactgccg ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc
     7441 gaaacgcaac ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacgg
     7501 cattcccttc tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt
     7561 gcgcataccg cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt
     7621 cctcatccct ttagaacctt tttggaactg gctgcaaacc ctgccgggca accacctcga
     7681 cggactgact cttgaagaca tccaagccga cgccaattcc taccttgtcc gcccgtgcga
     7741 aaccgccgac gaagtatggg acgaaatcga agcccgtttt gaagacattt tcgccgccga
     7801 acttgccgac tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa
     7861 cgaatggttc gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc
     7921 ccgcgaagcc ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat
     7981 ctcgacggct acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc
     8041 tgggcgtttt ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc
     8101 gcccgcatcg acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca
     8161 tgccgcctga aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca
     8221 aacggcaaaa ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa
     8281 cgcaccgtca gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa
     8341 aaatactcac cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc
     8401 ccgacagcaa aaccgcgccc gccttctccc tgcccgacct gcacggaaaa accgtttcca
     8461 acgccgacct gcaaggcaaa gtaaccctga ttaatttttg gtttccctcc tgtccgggtt
     8521 gtgtgagcga aatgcccaaa atcattaaaa cggcaaatga ctataaaaac aaaaacttcc
     8581 aagtccttgc cgtcgcccag cccatcgatc cgatagaaag cgtccgccaa tatgtcaaag
     8641 actacggttt gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg
     8701 gcacacaggt ttatccgact tccgtcctta tcggcaaaaa aggcgaaatc ctcaaaacct
     8761 acgtcggcga acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcacaat
     8821 agcgatgccg tctgaaacgc aaaaaaacag aaacgatgtt cctgtttttt cagatcatcc
     8881 gccccgacac gttcagacgg cattcaacct tccggcagat tctttttcac cactgccgga
     8941 ttgcctgccg ccaaagagta gggcggaatg tcttttgtaa ccaccgcgcc tgcgcccacg
     9001 accgaaccgc gtccgacggt tacgcccgcc attacaatca ccctgcgccc cggccagaca
     9061 tcgtcctcca acgtaatcgg acggatttcc gtgtagccct caaagcgttt tttgaacggt
     9121 caaacttgtg gttatttgaa taaaacagac attccggctc catcatcaca tttttgccga
     9181 ccaccggccc acggcagatt tcacagtttg ccccgatgcc cgagccgtcg cccaaaaccg
     9241 tatccggaaa cacatacgcc ccgcgttcga tattgacccc gcgtccaata tgcggagaaa
     9301 cccgccgcgc caaaaaaccg cgtacccgtc tgccgacgat gccgcgcata tgcgacggcg
     9361 gcaacaccga accggcaaaa cgcagcagca ggaaaaagaa ttttgatccc atcaaacctc
     9421 cctcaaaaga a
//
LOCUS       DAWKOD010000069         9323 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000069 DAWKOD010000000
VERSION     DAWKOD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.069"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..143)
                     /locus_tag="WJK73_001238"
     CDS             complement(<1..143)
                     /locus_tag="WJK73_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557718.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGL"
     gene            complement(161..718)
                     /locus_tag="WJK73_001239"
     CDS             complement(161..718)
                     /locus_tag="WJK73_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7557719.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMSPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDVEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WJK73_001240"
     CDS             complement(794..2428)
                     /locus_tag="WJK73_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ7557720.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2502..2831)
                     /locus_tag="WJK73_001241"
     CDS             complement(2502..2831)
                     /locus_tag="WJK73_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557721.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WJK73_001242"
     CDS             complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WJK73_001242"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ7557722.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4302..4733
                     /gene="rimP"
                     /locus_tag="WJK73_001243"
     CDS             4302..4733
                     /gene="rimP"
                     /locus_tag="WJK73_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ7557723.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4761..6263
                     /gene="nusA"
                     /locus_tag="WJK73_001244"
     CDS             4761..6263
                     /gene="nusA"
                     /locus_tag="WJK73_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ7557724.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6275..9163
                     /gene="infB"
                     /locus_tag="WJK73_001245"
     CDS             6275..9163
                     /gene="infB"
                     /locus_tag="WJK73_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ7557725.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2499 a         2689 c         2359 g         1776 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttccgccga acgcatcggg cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattttgctt gagattccgt ttaccaatga ctgacaaacg
      181 cttccacatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt accccgataa ccatcggcga catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttt aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacgataaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggggctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttgact gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aagtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccgac cgggcggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcc gccgccgtgg
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 aggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggcg gctgtgcggg ggattccgcc cgccattcaa acgtccggca
     2581 gacataaaaa catcgtaagc aagattcgaa ccggtctgca accgcccctg ccagaaaaac
     2641 gggcaaagca tttcatattg gaaaaacccg gccgcgccgc cgacgggaca gtccggcaca
     2701 aacagcatca cgcccggatt gaaaaggacg gatagccgcc ggcagcacaa tcttaccacc
     2761 tccaaaacgc cgccggagaa cgccgaaaca gccgatgccg tctgaagccg cttcagacaa
     2821 catcgggaca tcaaccgtaa cgccgttgga aatcgcgcat aaaatccgcc aaagcccgca
     2881 cgccttcaag cggcatcgca ttataaatgc tggcacgcat accgccgacg gttttatagc
     2941 ccttaagcag gcacaagccc tgcaattcgg cttccagcac aaaacggcgg tcaagctcct
     3001 catcccccgt ttggaacacg acattcattt tagaacgcgc attcggacgg atacggttga
     3061 tatagaaacc gccgctgccg tctatcgtct catacaaggt ttgcgccttc agccgattga
     3121 ccgcttcaat ttttttcaca ccgccctgcg cctgcagcca gcggaacacc agccccgaca
     3181 tataaatcgc gtaagttgac ggcgtgttgt acataccttc gcggttcaaa tgcgaacggt
     3241 agttgaacac atcgggaata tcgttcggac aacgctcgag caaatcctca cgcacaatca
     3301 ccaccgtaac ccctgccggc ccgatgtttt tctgtgcgcc tgcgtaaatc agtccgtagt
     3361 cggcaacatc aaactcgcgc gacaaaatct cgctggacat atcgcacacc agcggcggca
     3421 tgccttctga aaggcgcggc acttcacggt attgcagccc gttgaccgtt tcattgacgg
     3481 caaaatggac aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc ggcaggtcga
     3541 gatagtcgaa ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg gtcaaacggc
     3601 tcatctgttc ataagcgata cggctccagt tgcccgttac caccgcgtcg gcagtgcgga
     3661 aaccgtgtgc cagattcatg gctgccatat taaattgggt tgttgctccg ccctgcagaa
     3721 acaatatctt atagttgtca ggcactttca aaagctgcct caaatcctgt tccgcatgat
     3781 gcaggatgct caaaaacatt tccgaacggt ggctcattgc catcacagga aaacccgtac
     3841 cgttgtagtc caacatttcc tgccgcgccg tttccaacac ggcttcgggc aatacggcag
     3901 ggccggcgga aaaattgtaa atcggataaa gagacatgat gcagccttga ttctgaacaa
     3961 taacccgccc gattttaggc ttgcagcagg ctttgtgcaa ggatggaaaa tacctgtcct
     4021 ccgccccgct tcatgccgcc cgaacacaga aaacaacatc aatatattga tttacaaaca
     4081 taaaaatcat gcacgcgaca aatagataca tttgttttgt caacaatatt cacgatttcc
     4141 cattacaaac ctcccttaca tccgcttttt tccgtcccaa aaacacaaaa taaatcaaca
     4201 ctttcatttc tccgcaaaag cggttataat tacgccgatt tttcaacttt gacgaaaaat
     4261 ggccacgggg ccattttttt attatgtaca tagggagcag catggatatc caaaccatcc
     4321 tcgaaaaaac cctgcccggc ctgggctacg aactggtcga ttttgaactg accgcgcaag
     4381 gaacattgcg cgtgttcatc gacaaagaag gcggcattac cgtcgaagac tgcgcaaccg
     4441 tcagcaacca cttgagccgc gtcttcatgg ttgaagacat cgactacaaa aacctggaaa
     4501 tttccagccc cggactcgac cgccccttga aaaaagccgc cgacttcgtg cgctttgccg
     4561 gtcagaatgc caaaatcaaa acccgcctgc cgatagacgg tcaaaaaaac tttatcggta
     4621 aaatcgaagg ctgcgaaaac gacactgtta ccgtttcctt cgacggcaaa accgtacaaa
     4681 tcgaattggg caacatcgac aaagcccgcc tgcgccccga attcaaattc taaaacacaa
     4741 caatattgga gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa gcactggcaa
     4801 gcgaaaaaaa cgttgatgcg gaagtcgtct tccaagcact ggaattcgcc ctgtctaccg
     4861 ccgccaagaa aaaggcagac cgcgaacaca tggacgtgcg cgtccaaatc aaccgcgaca
     4921 ccggcgaata ccaaaccttc cgccgctggc tgattgtcgc cgatgaagac tatacctatc
     4981 ccgatgtcga aaaaaccatc gaggaaatcc aagaggaaat tcccggcact accatccaaa
     5041 tcggcgaata ctacgaagag cagctaccca acgaaggctt cggccgccaa gccgcgcaaa
     5101 ccgccaaaca aatcatcctg caacgcatcc gcgatgcgga gcgcgagcaa aatctgaacg
     5161 agtttctcgc cgtcaaagaa gacatcgtgt ccggcacggt caaacgcgtc gaacgccacg
     5221 gcatcatcgt cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc gaccaaatga
     5281 ttccgcgcga aaacttccgc agcggcgacc gcatccgcgc cctcttcctg cgcgtcgaag
     5341 aaatcggcaa caccggccgc aaacaagtca tcctgagccg tacttccggc gatttcctcg
     5401 tcaaactgta cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa atccgcgctg
     5461 tcgcccgcga cccgggacaa cgtgccaaag tcgccgtcaa agccaacgac cagcgcatcg
     5521 atccgcaagg cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc agcaacgaat
     5581 tgtccggcga gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa ttcgtcatga
     5641 gcgcgctctc acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa cacgccgtcg
     5701 atgtcatcgt tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt caaaacgtgc
     5761 gccttgcttc cgacctgacc ggctggcagc tcaacatcat gacttccgcc gaggcagacg
     5821 aacgcaatgc ggcagaagat gccgccatcc gccgcctgtt tatggatcac ttgaacgtgg
     5881 acgaagaaac cgccgacgta ctggttcagg aaggttttgc aaccttggaa gaagtcgcct
     5941 atgttcctgc cgccgaactg cttgccattg aaggatttga cgaagaaatc gtcgatatgc
     6001 tccgcaaccg cgcccgcgat gccatcctga ccatggcgat tgccgccgaa gaaaaactgg
     6061 gcgaagtatc cgacgatatg cgcaacctcg aaggcataga tgccgatatg ctccgcagcc
     6121 ttgccgaagc aggcattacc acccgcgacg acttggcaga gcttgctgtg gacgaactga
     6181 ttgaaatcac cggtgtaaac gaagaaaccg caaaagccgt catcctgacc gcacgcgaac
     6241 actggtttac cgaagacaaa taaagggggt acagatgagt aacacaaccg tagaacaatt
     6301 tgccgccgag ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt
     6361 cagcaaaaac agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta
     6421 cctgaccaag aaaaacggca gcaacggcgg caccatcagc atccgccgca ccaaaaccga
     6481 agtcagcacc gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat
     6541 tccttctgcc gaagaattgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt
     6601 ccggccggag cagacggcag aagacgcggc aaaagcccga gccgaagctg ccgcacgtgc
     6661 agaagcccgt gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa
     6721 caaagccaaa cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc
     6781 ggcggaaacc aaacccgccg aagaaagcaa agcggaaaaa gcccaagccg acaaaatgcc
     6841 gtctaaaaaa cccgtcgagc ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg
     6901 caaaggcaaa gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt
     6961 ggtcagcgcg gaagaacagg cgcaacgcga tgaagaagca cgccgtgccg ccgcacttcg
     7021 cgcccaccag gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat
     7081 gaaacaacag gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg
     7141 tcccgccaaa cctgccgaga aaccgcaggc agccgcgccg gccgtcgaaa acaaacctgt
     7201 caatccggca aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg
     7261 caacgccaaa ggcaaaggcg gaaaaggcgg gcgcgaccgc aacaatgcac gcaacggcga
     7321 cgacgagcgc gtacgcggca gcaaaaaagg caaaaaactc aaactcgagc cgaatcaaca
     7381 cgccttccaa gcaccgaccg aacccgtcgt tcatgaagtt ttggttcccg aaaccattac
     7441 cgttgccgat ttggcgcaca aaatggcggt caaaggcgtg gaagtggtca aagccctgat
     7501 gaagatgggc atgatggtaa ccatcaacca atccatcgac caagacaccg ccctgattgt
     7561 cgtcgaagaa ctcggacaca tcggcaaacc cgccgccgcc gacgatcccg aagccttctt
     7621 ggacgagggc gcggaagcgg tggaagccga agcattgccg cgtccgcccg tcgttaccgt
     7681 catgggacac gtcgaccacg gcaaaacctc gctgctggac tacatccgcc gtgccaaagt
     7741 ggtacagggc gaagcgggcg gcattaccca acacatcggt gcataccacg ttgaaacccc
     7801 acgcggcgtg attaccttct tggatactcc gggtcacgaa gcgtttaccg ccatgcgcgc
     7861 gcgcggcgcg aaagcgaccg acatcgtgat tctcgtggtt gccgcagacg acggtgtaat
     7921 gccgcaaacc atcgaagcca ttgcacacgc caaagcggca ggcgttccga ttgtggttgc
     7981 cgtcaacaaa atcgataaag acactgccaa ccccgaacgc atccgccagg aactgaccca
     8041 acacgaagtg atccccgatg attggggcgg cacggttcaa ttcatcgacg tttctgcgaa
     8101 aaaaggaaca aacatcgacg cgctgctcga agcagtcttg ctcgaagccg aagtattgga
     8161 actgaccgca cctgtcgatg cgcccgccaa aggcatcatc gtcgaggcgc gcttggacaa
     8221 aggccgcggc gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgacat
     8281 gctgctggcc ggtacggcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc
     8341 cattaccgaa gccggcccgt ccatccccgt cgaaatcctc ggcttgtccg acgtaccgaa
     8401 tgcgggtgaa gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt
     8461 ccgccaaggc aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa
     8521 tatgttcaac aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga
     8581 cgtgcagggc tcttacgagg ctttggcggg cagcctgaaa aaactgtcca cagacgaagt
     8641 gaaagtgaac gtgttgcaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc
     8701 catcgcttcg ggcgcattca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa
     8761 acttgccgaa aatgaaaacg tggaaatccg ctactacaac atcatctatg atgccatcga
     8821 cgacgtgaaa gcggcgatga gcggtatgct ttctcccgaa gagaaagaac aggttaccgg
     8881 tacggtcgaa atccgtcagg ttatctccgt ttccaaagtc ggcaatattg caggctgtat
     8941 ggttaccgac ggcgtggtca aacgcgattc ccatgtccgc ctcatccgca acaacgtggt
     9001 tatccacacg ggcgaactgg cttcgttgaa acgctataaa gacgatgtaa aagaagtccg
     9061 catgggcttc gagtgcggcc tgatgattaa aggctacaac gaaattatgg aaggcgacca
     9121 actcgaatgt ttcgacatcg tcgaagtggc acgcaccctg tgattttgat gcggaaaaat
     9181 gccgtctgaa gcgttcagac ggcatacgaa acgggttctg tatcatacag aacccgtttt
     9241 ttgtcgcaaa tcggcttcag acagccctct tgccttatcc cgatttgaat ctgacttgcc
     9301 atacaaacag gcttcagacg gca
//
LOCUS       DAWKOD010000070         9322 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000070 DAWKOD010000000
VERSION     DAWKOD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.070"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            164..1507
                     /locus_tag="WJK73_001246"
     CDS             164..1507
                     /locus_tag="WJK73_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ7557726.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="WJK73_001247"
     CDS             1510..2742
                     /locus_tag="WJK73_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ7557727.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="WJK73_001248"
     CDS             2735..3511
                     /locus_tag="WJK73_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ7557728.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="WJK73_001249"
     CDS             3511..4137
                     /locus_tag="WJK73_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ7557729.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="WJK73_001250"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="WJK73_001250"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ7557730.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="WJK73_001251"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="WJK73_001251"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ7557731.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6118..7173
                     /locus_tag="WJK73_001252"
     CDS             6118..7173
                     /locus_tag="WJK73_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7557732.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7199..7414
                     /gene="nqrM"
                     /locus_tag="WJK73_001253"
     CDS             7199..7414
                     /gene="nqrM"
                     /locus_tag="WJK73_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ7557733.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WJK73_001254"
     CDS             complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WJK73_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ7557734.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8300..9118
                     /gene="cysE"
                     /locus_tag="WJK73_001255"
     CDS             8300..9118
                     /gene="cysE"
                     /locus_tag="WJK73_001255"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ7557735.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2253 a         2595 c         2323 g         2151 t
ORIGIN      
        1 aacagcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg ggcccgtcat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 acttcagtcg gtcgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga tccaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctagacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaagcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgcgttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgttgt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tctccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcga ccgaccccgt ttccgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcactgatcg gtgtgatgtg cgtattaatc cgcgtggtca atccggctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta tttcgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tcgtgttggc ggtcagcctg atttgctcgg tcatcgttgc
     2821 cggtgcggtc gtcggcttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaggacacc gacatcggta aaacttttgc
     2941 cgagcgtatc gagcaacgcg tggtcgattt ggcgaccggc gaatatgtga aagacgcgcc
     3001 gaaagacttc agtgcgcgca tcgcaggcaa agaccccgcg caaagcatcc gcatcaaacc
     3061 cgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt acttggtaaa
     3121 aggtgaagac ggcaaaatcg gccaaatcat cctgcctatg cacggtaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccaacc cgacggcaac accatcaacg gcattaccta
     3241 ctacgagcaa ggcgaaaccc cgggcttagg cggcgaaatc ggcaatcctt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggcgcgt cgctgacatc
     3421 caaaggcgta caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacatttg
     3541 atgttttcac cctttatcga caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgcatcatcg tgcaaatggc gattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gcatcggcaa cggcgcgggc tacgggatga tgctgcttgt cgtcgccacc
     3961 gtccgcgaac tgattggctc gggcaaactc ttgggctaca ccgttttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct ctttatcaaa tccgtcttca tcgaaaatat ggcgctatcc
     4201 ttctttttgg gtatgtgtac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtgatttt cgtactcgga ctgtccgtcc ccgccaacca attggtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctataacgc gctgggcatc tacctgccgc tgattaccgt aaactgcgcg
     4501 attttcggtg ccgtatcgtt tatggcgcag cgcgaatacg gcttcggcga atccgtcgtg
     4561 tacggcttcg gtgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctctg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc gtctgtcgtg ccaagtcaac gtcaaaaccg acatggacat
     5101 cgaagtcccc gaagaagtgt tcggcgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 taacaaagcc acgttcatta aagaactcaa gcttgccatt cccgaaggcg aagaagtccc
     5221 cttccgcgcc ggcggctaca ttcaaattga agccccgccg cacaccgttg cctacaaaga
     5281 cttcgacatt cccgaggaat accacgaaga ctgggacaaa tacaatttgt ggcaatacgt
     5341 ttccaaagtg gacgagccga ttttgcgtgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgccgccc ccgcgcgtac ctgatgcgcc
     5461 tccgggacaa atgtcgtcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 cggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgtaag attacctttt ggtacggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agacttcgac caactcgcag cagaattccc gaacttcacg tggcacgtcg ccctgtccga
     5761 cccgctgccg gaagacaact gggacggcta cacgggcttc atccacaacg tggtttacga
     5821 aaaccatctg aaaaaccacg aagcaccgga agactgcgaa ttttatatgt gcggtccgcc
     5881 aatcatgaac cagtccgtga tcaaaatgct caaagacttg ggcgtggaag acgaaaacat
     5941 cctcttggat gatttcggcg gttgagtatt cggcttcaaa tacaaaagga agaaacctcg
     6001 gtttcttcct ttttctatgc cgatgccatc cgaacaccct tcagacggca tcggcataga
     6061 aacataccgg ccgttttgcg atataattca aaccatttgc caaaaccgtc aaacactatg
     6121 ccgtctgaaa cacgcctgcc gaactttatc cgcaccttga tatttgccct gagttttatc
     6181 ttcctgaacg cctgttcgga acaaaccgcg caaaccgtta ccctgcaagg tgaaacgatg
     6241 ggcacgacct ataccgtcaa atacctttca aacaatcggg acaaactccc ctcccctgcc
     6301 gaaatacaaa agcgcatcga tgacgcgctt aaagaagtca accggcagat gtccacctat
     6361 cagcctaatt ccgaaatcag ccggttcaac caaaccacgg ccggcaaacc cctccgcatt
     6421 tcaagcgact tcgcacacgt tactgccgaa gctgtccgcc tgaaccgcct gacacacggc
     6481 gcgctggacg taaccgtcgg ccccttggtc aacctttggg gattcggccc cgacaaatcc
     6541 gttacccgtg aaccgtcgcc ggaacaaatc aaacaggcgg catcttatac gggcatagac
     6601 aaaatcattt tgaaacaagg caaagattac gcttccttga gcaaaaccca ccccaaggcc
     6661 tatttggatt tatcttcgat tgccaaaggc ttcggcgttg ataaagttgc gggcgaactg
     6721 gaaaaatacg gcattcaaaa ttatctggtc gaaatcggcg gcgagttgca cggcaaaggc
     6781 aaaaacgcgc gcggcgaacc gtggcgcatc ggtatcgagc agcccaatat cgtccaaggc
     6841 ggcaatacgc agattatcgt cccgctgaac aaccgttcgc ttgccacttc cggcgattac
     6901 cgtattttcc acgtcgataa aaacggcaaa cgcctctccc atatcattaa tccgaacaac
     6961 aaacgaccca tcagccacaa cctcgcctcc atcagcgtgg tcgcagacag tgcgatgacg
     7021 gcggacggct tgtccacagg attattcgta ttgggcgaaa ccgaagcctt aaagctggca
     7081 gagcgcgaaa aactcgctgt tttcctgatt gtcagggata aaggcggcta ccgcaccgcc
     7141 atgtcttccg aatttgaaaa actgctccgc taaacataca aaccgattag gaaacaccat
     7201 gaaaaccctg ctccttacct tcggcatctt cctgaccgtc atcatcggta tggcggtcgg
     7261 ctatattttc tccaaacgca gcatcaaagg cagttgcggc ggcattactg ccttgggtat
     7321 gaaaaaaatg tgcgactgcg acacaccttg cgacaccctg caaaagaaac tggatgaaga
     7381 aaaacaggca ggcggcatac gggttgaccg ttaacgccga aacaaaatgc cgtctgaagc
     7441 ccgacaggcg ggttcagacg gcattgccgc tttacttcga tcggtttata cgcaaaacgc
     7501 gcttcaggaa ataaatcaga ttattcccca gacgccttca ggcttccttc tgtgccaccg
     7561 taaccatagc cgggcgcaac acgcggtcgg acagcgtata acccttcttc atcacgccca
     7621 ccacggtatt gggttcctgt tcgcttgcca ccgcctgcat cgcctgatgg atattcggat
     7681 cgagcttatc gcccgcttta ggattgattt ccttgatttg cgtagcatca aatgctttct
     7741 gcaactcgtt caaagtcatc tgcacgccca ttttcagcgc atcgaaattg ccgctctgat
     7801 ccaaaagcgc catttccaga taatccttga ccggcagcat ttccacggca aacttctgtc
     7861 cggcgaactt gtgcgtatcc gcaatttcct gctgatggcg gcggcgcagg ttttgctcgt
     7921 ttgccaaagc gcgcagctgc tcgtctttca actgcgcttc cagctcggca atccgcgcct
     7981 gcaaatcctc ataagccggc tctgcggcag cctgttcctg cacaccgtcc gcatttccta
     8041 ctgtctcgac ggtttccacc gcctccacat tttcaaccgc ttcttcactg ttttgctgct
     8101 gtgtctgttc gctcatatcg tatcccttaa aacaaattgg aaatcaaaat ccagttatta
     8161 cccgcgcaag ataaggacat tttcaagaaa cttcaagcaa aggcagagaa tttcaaattc
     8221 attttcaaat cccctaccga aaaaataata tagacggtaa gatactgccg accatatcga
     8281 ttttcaaacg tcatcactca tgaaaaaaga ccatttaaac acaaccggtt tcgacctctg
     8341 gcacaccatc cgcgaagaaa ccgcggccgc ggtctccgcc gaaccgatgc tggcaagttt
     8401 tttgcaccaa accgtgttgc gccacgagtc cctcggctcc gtccttgcct accacctttc
     8461 cagcaaactc ggcagcccga ttatggacgt gcgcgcgctg tttgaaatct accagcaggc
     8521 gttgggcagc gacacccaaa tcggcaaatg cgtcgaagca gacttaaaag ccatctacga
     8581 acgcgatccc gcctgcgacg aatattcgct gccgctttta tatttcaaag gcttccacgc
     8641 gattcaggca caccgcatca accatcggct gtatctcgac gggcgcaaaa cgctggcgta
     8701 tttcctgcaa aaccgtatgt ccgaagtatt cggcgtggac atccatcccg ccgcccgttt
     8761 cggatacggg ctgatgctcg accacgccac cggctttgtt gccggcgaaa ccgccgtgtt
     8821 gggcaacaat atttcgattt tgcacggcgt aacgctcggc ggttcgggca aagaaggcgg
     8881 cgaccgccac cctaaaatcg gcgacggcgt gatgatcggc gcaaacgcct cgatattggg
     8941 caatatccgc atcggcagca atgccaaaat cggggcgggc agcgtcgtgg tttcagacgt
     9001 gccgccgtcc atcacggttg tcggcgtacc cgccaaaccc gtggcgcgat cgctcaaaac
     9061 accgtcggcg gatatggatc aaaatatcca gtttaccgaa atcgacttta tgatttgaat
     9121 atccgcacca atgccgtctg aaacgccaaa cgggcttcag acgacattgc ccgcttcaag
     9181 cctgcgcctt gatatgccgc cgatgtcggc acttgccagt cggacaaaat gccttttatt
     9241 cgatacaccc gattcccacg caaccggaaa tatagtggat taacaaaaat caggacaagg
     9301 cgacgaagcc gcagacagta cg
//
LOCUS       DAWKOD010000071         9272 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000071 DAWKOD010000000
VERSION     DAWKOD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.071"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            88..297
                     /locus_tag="WJK73_001256"
     CDS             88..297
                     /locus_tag="WJK73_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ7557736.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            386..1600
                     /locus_tag="WJK73_001257"
     CDS             386..1600
                     /locus_tag="WJK73_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ7557737.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="WJK73_001258"
     CDS             complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="WJK73_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ7557738.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSSQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEANGDRL
                     KFA"
     gene            4526..5536
                     /gene="trpS"
                     /locus_tag="WJK73_001259"
     CDS             4526..5536
                     /gene="trpS"
                     /locus_tag="WJK73_001259"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ7557739.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5542..5883
                     /locus_tag="WJK73_001260"
     CDS             5542..5883
                     /locus_tag="WJK73_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557740.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHPNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6205..6528
                     /locus_tag="WJK73_001261"
     CDS             6205..6528
                     /locus_tag="WJK73_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557741.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEVKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="WJK73_001262"
     CDS             complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="WJK73_001262"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ7557742.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8361..9093)
                     /locus_tag="WJK73_001263"
                     /pseudo
     CDS             complement(8361..9093)
                     /locus_tag="WJK73_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         2137 a         2399 c         2450 g         2286 t
ORIGIN      
        1 atttgaattt ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat
       61 agcaatattt caaaacggag aaagatcatg ccttatgtca atattaaagt aaccggcggc
      121 aaggaagcac cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaac cgaattgctg
      181 gcacgcgtgc tgggcaaaaa tcccgaaaca acggttgtcg tgattgatga agtggatacc
      241 gataactggg gaataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa
      301 gcctgaaaat ctcggtttga tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca
      361 ttgcccaaaa tttacggaac acatcatgga taaatttccc aagtctgcaa agctcgatca
      421 cgtctgttac gacatacgcg ggccggttca caaaaaagcc cttcagttgg aagaggaggg
      481 caataaaatc cttaaactca atatcggcaa ccctgcgccg ttcggctttg aagcccctga
      541 tgaaatcttg gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattctaa
      601 agggctgtat tccgcccgca aagccattgt tcactactat cagaccaaag gtttgcgcga
      661 tattacggtt gatgatgtct atatcggcaa cggcgtgtcc gagctgatta cgatgtctat
      721 gcaggcattg ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg
      781 gacggcggcg gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa
      841 cggctggttc cccaaccttg ccgatatgga agccaaaatc acgcccaaaa ccaaagccat
      901 cgtcgtcatc aatcccaata atccgacagg cgcggtgtac agcagggaaa tcctgttgga
      961 aatcgccgaa ctggcgcgca agcacggttt gattattttc gccgacgaga tttacgataa
     1021 aatcctttat gacggcgcgg ttcaccacca catcgccgcg cttgcccccg accttttgac
     1081 ggtaacgttc aacggtttgt ccaaatccta ccgcgtcgcc ggcttccgcc aaggctggat
     1141 ggtgctcaac gggccgaaac atcacgcaaa aggttacatc gagggtttgg atatgctctc
     1201 gtctatgcgc ctgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg
     1261 ctatcagagc atcaacgaat ttattttgcc cggcgggcgg cttttggaac agcgcaacag
     1321 ggcgtgggaa ctggtcagcc aaatccccgg cgtgtcctgc gtcaaaccga tgggtgcgat
     1381 gtatatgttc ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgaca tgaaattcgt
     1441 ttacgatttg ctggtgcgcg aaaaagtctt gctggtacag ggaacggggt ttaattggat
     1501 caagcccgac catttccgca ttgttacgct gccttacgtc catcagattg aagaggcgat
     1561 gggcaggctg gcaagattcc tgcaaaccta ccgccaataa ggggacggtt tgtctgccga
     1621 ggataaaaaa tgccgtctga aacggagatt cccgtttcag acggcatttt caacagcagg
     1681 aacgaatcag gcaaatttca gtctgtcgcc gttggcttct accctgattt cgctttcggg
     1741 cgcatagttt ccggcaagca gagcttttgc cagcgggttt tcgatttccg actggatggc
     1801 gcgtttgagc ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag
     1861 agcggcatcg gaaacagtca ggcggaggtt ttgtttttcc aaacgttttt ccaaaccttt
     1921 aagctggatt ttggcaatac tgcggatgtt tgcctgatcc agtccgtgga acacgaccac
     1981 ttcgtcgatg cggttgatca tttcaggacg gaaatgttct ttcacatcct ccatcacaac
     2041 ttctttcacc gcttcgtagt cttgagtacc catttgttgg atgtgctggc tgccgatgtt
     2101 ggaagtcatc acaatcacgg tatttttaaa atctaccgtc ctgccttgtc cgtcggtcaa
     2161 gcggccgtca tccaatactt gcagcaggat gttgaacaca tcgggatggg ctttttccac
     2221 ttcgtccaaa agaattacgc tgtacggttt gcggcggact tgttcggtca ggtagccgcc
     2281 ttcttcgtag ccgacatagc ctggaggcgc gccgatgagg cgggcaacgg cgtgtttttc
     2341 catatattcg gacatatcga tacgaatcag atgatcttca ctgtcaaaca ggaagcctgc
     2401 caatgctttg cacaactcgg ttttacccac gccggtcggg cccaagaaca ggaagctgcc
     2461 gtaaggttta ttcgggtcgg caagaccgga gcggctgcgg cggatggcgt cggacacggc
     2521 acgcacggct tcgtcttgac cgactacgcg gcggtgcaac acttcttcca ttttcagcaa
     2581 cttgtcgcgt tcgccttcca tcatttttga cacaggaatg ccggtcatac gggaaaccac
     2641 ttctgcgatt tcctctgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct
     2701 gtccgtatct gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata
     2761 catcaatttt gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt
     2821 gacttcgtca atttgtttct taatattagc agcaccgtct gaaattgctt tttcggcttt
     2881 ccagatttcg tctaaatcgg cgtattcttt ttgcagaccg ttgatttcct cgtctatcag
     2941 ttccaaacgt tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg
     3001 aagctgaatt agacggcggt cgattttgtc cattgcttcc ggcttggttt ctttttccat
     3061 cttgacacgg ctggcggctt cgtcaatcaa atcaatcgct ttatcgggca ggaagcggtc
     3121 ggtgatgtag cggtcgctca actctgcggc ggcaacaatg gcggggtcgg tgatgtcgat
     3181 gccgtggtgg atttcgtagc gttcctgcaa accgcgcaag atggcgatgg tgtcttccac
     3241 actgggttcg ccaaccaata ctttttggaa acggcgttcg agtgccgcgt ctttttcgat
     3301 gtattggcgg tattcgtcca aagtagttgc accgatacag tgcaattcgc cgcgtgccaa
     3361 agccggtttc aacatattac ccgcgtccat cgcgccgtcg gttttacctg caccaaccaa
     3421 agtatgaatt tcgtcaatga aaatcagagt gttgccatcg tctttagcca agtcgttcaa
     3481 cacgcctttc aagcgttctt caaattcgcc gcggtatttc gcgccggcaa tcaatgccgc
     3541 caagtccaaa accagtaggc gtttgttgcg cagggattca ggcacttcgc cgttgacgat
     3601 acgttgcgcc aagccttcaa caatggcggt tttacccacg cccggttcgc caatcagcac
     3661 ggggttgttt ttggtgcggc gttgcaatac ctgaattgca cggcggattt cgtcgtcacg
     3721 accgataacg gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt
     3781 tttcagcgca tcgcgttggt cttcggcatt ggcatcgttc acgttttgtc ctcctcgtac
     3841 cgcgtcaatc gcggcattga tgttttgttc ggtcgcgccg gcttctttca agattttgcc
     3901 ggtcgcatcg ttctgctgca ccaaggcgag caggaaaagt tcgctggcga tataggcatc
     3961 gccgcgtttg gttgcggctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg
     4021 cagaatgtcg ccgccctggc tggacacttt cggcaggctg ttcaaatgct gctgcaaacg
     4081 ctgtttcacc tgcggcacgt tcacgcccga atgcgccagc aacgcggcgg ctccgctgtt
     4141 ttggtcgtca agcagggctt ttaacacaaa gcctgcttcc agatagctgc cgtccgcagc
     4201 caacgccaaa ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta
     4261 tcgcattttt gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg cataattgtg
     4321 gataactcaa gttctgtttt ctgtttttct atatttaatt cgatatatca ttgaatttaa
     4381 agtatataaa aatgtataat aatgtgtata actatatctt cttaatatgg aaaagtctgt
     4441 tgtcggctgg atgtaggtgg caaatcgggt ataatcggca catctttttc cctttcagac
     4501 ggcattgatg ccgcaaggac attttatgag caaaaaacga gttctgaccg gcgtaaccac
     4561 caccggcatc ccgcatctgg gcaactacgt cggtgctatc cgccccgccg tccgcgcggc
     4621 gcaaaaccct gataccgaat ccttcctctt cctcgccgat taccacggta tcatcaaatg
     4681 ccacgagccg gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg
     4741 cggactcgac cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga
     4801 attgaactgg attctgacct gtatcaccgc caagggtttg atgaaccgcg cccacgccta
     4861 caaagccgcc gtgcaggcaa attcagaaaa caatcaggaa gatcccgatt tcggcgtgga
     4921 aatgggtttg ttcagctatc cgattctgat gaccgccgac atccttatgt tcaacgccaa
     4981 cgaagtgccc gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acatcgccgg
     5041 ccgcttcaat caccgcttta aagaaatctt taccctgccc gaagtgaaaa tcgacgaaaa
     5101 cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaacaccat
     5161 tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca tcaccaatat
     5221 gaaagagccg ggcgagccga aacagcccga cgaaagccca ttgtttgaaa tctacaaagc
     5281 cttctccacg ccgtctgaaa ccgcagcgtt cacgcaaatg cttgccgaag gcttggcttg
     5341 gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc gaactcgccg aaccgcgcga
     5401 acgctacaac gagctgaccg ccgacccttc gcaaatcgaa gagattttgc aggcaggcgc
     5461 gcaaaaagcg cgtaaagaag cacgcgaatt gttggacaaa gtacgcgatg cggtcggcat
     5521 ccgcccgttg aaataaaccc gatgccgtcc gaacctctta aaggcttgcc ggaacaaaac
     5581 gtcgtccggc tgatcggcaa gcatcccaac gacttggaag ccgtcgtcgg caaatgtatg
     5641 gaaaccgacg gaaagggcgc gccttcgggc tgggcggcaa acggcgtgtg ccataccttg
     5701 tttgccaaac tggtgggcaa tatcgccgaa gacggcggca aactgacgga ttacctgatt
     5761 tcgcattccg ccctgcaacc ctatcaggca ggcaaaagcg gctatgccgc cgtgcagaac
     5821 ggacgctatg tgctggaaat cgacagcgaa ggggcgtttt atttccgccg ccgccattat
     5881 tgaggtattc ggacatcccg gaatatattt gggtttttca aaccctgcgg gaaaaaagtc
     5941 cgcaccgtcg ggaaactcaa aaggaggggg atatgtgtta caattttccg aactgttttc
     6001 ataaaatagt tttcggacgt gtttcaatat ggcattgatg ccgccttatt gttcgagaaa
     6061 aaaaaccttt atatttaaat ataatgggtt ttaactaaac gggaaaccgt tttctctccg
     6121 gccgatgggc aaaatcagcc gattgatgga acacggtccg atttttaagc aaaaccttaa
     6181 tcaacaactc aagaaggata agacatgaaa aaattattga ttgccgcaat gatggcggct
     6241 gccttggcag cttgttcgca agaagccaaa caggaggtta aggaagcggt tcaagccgtt
     6301 gagtccgatg ttaaagacac tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa
     6361 gaagtgaaag accaagtcaa agatgctgcg gctgatgcaa aggcaagtgc cgaggaagct
     6421 gtaactgaag ccaaagaagc tgtaactgaa gcagctaaag atactttgaa caaagctgcc
     6481 gacgcgactc aggaagcggc agacaaaatg aaagatgccg ccaaataatt tgttgccttg
     6541 gcaaaaatga tgggatgccg cctgccggca acccaaacga aaccgcctga agattttcag
     6601 gcggtttttg agttgtatgc cccttgtttt ttacgcctgc atgaccgttc cgagcagatg
     6661 tgccgcgcat atcgggatgt tgcggacagg caaatgccgt ctggacaggg tttggacggc
     6721 atttgttccg taaggttcag acgacctcga ctttaaacgg catattgatt ttttgccatt
     6781 ggacgctttc atattccaat gcgtcggcaa tcaggggatg gcgttccagc cattccctgt
     6841 caatacgcag gatgaagccg cagctttccg tatccgtgcg caactgcata tttttcggga
     6901 aagacaggtc ttggcgcgaa cggcagaaca gggcagcaag gcgcagggac aaaacggcat
     6961 accacaacat ttcgttggtg ccgatgatgc cgctcatttt tttcatatcg ccgcgatgac
     7021 cgatgaccag ttgggcaagt atggtctgtt ctttgcgtga gaaacccggc atatcggcgt
     7081 tttcgaggat gtaggcggaa tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt
     7141 cgtgcagcgc ggcggcgcgt ccgagatact gttgccacaa ggcaagctct tgaactgtaa
     7201 cgtttttagc gtggcagagg ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg
     7261 cctgattgag gctgacgtgg tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat
     7321 cttcgtttaa accgcgcccg atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg
     7381 ttacggtcat cctgtcgagt ttcatttcct caaacgccgc catcatcacg gcaagtccgc
     7441 cggcaaaaac ttcgatgcgt tccggtttca ggttttcaaa tttggctttt ttgaccgaac
     7501 cggcttcgat gatgcgttcg gagagggcgc gcataccttt gtaggtaatg tccgcctctt
     7561 ggggcatttc ggcggcaagc acgtcgcgga tggattttgc cgaacccgat gtgccgacgg
     7621 cgaaatccca gccttcgcgc ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg
     7681 cggaaatggc ggcttggaag tctttggcgg tgattttgtt ttggaagaag cgcaggctgt
     7741 aggttacgca gcccaagggc aggctttcgg taatgtcggg attcagcgtc gaaccgatga
     7801 caaattctgt cgaaccgccc ccgatgtcga taaccagcat tttgccgccg ccggggggga
     7861 gggtgtggat cacgccggta taaatcagcc gtgcctcttc gcgcccggcg atgatttcga
     7921 tggggaaacc cagtgccgct tcggctttgg gaaggaaatc tgcgatgttt ttggcgacac
     7981 ggaatgtatt ggttgccacg gcgcgtacct gttcagggcg gaagccgcgc aggcgttcgc
     8041 cgaattttgc cagacagtcc aaagcctgtt cttgggaagc ggcactcagg tttttctgtt
     8101 cgtccagtcc ggcggcgaag cgcaccatct gtttgaacga atcgatgact ttcaactgtc
     8161 cgttgttgtt ttcgcaaatc tggaggcgga aactgttaga acccaaatcg acggaggcaa
     8221 ggacgtttgc gggggtggtg gtcatggcgg ataccggtgg gggaaaaacg caatgttact
     8281 ctgacggcgc aggcgttgac aataaatgat gcggcggttt ttgattctgc ccacggatgt
     8341 tgccgacggc atttttgcgc ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg
     8401 tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg gaaattgagt
     8461 tgcggcgtaa agcccttcca agagattttg tcgtgccaca acgacaggtt tgcctgatat
     8521 tcgtggtctt tgcgcgggaa gcggtacaca atggtcccgg gtgcgtcaaa catcctgcgg
     8581 gtatagcgca ggtttgcccg cagacccaag cccgccgtcg aacgttttga ctgcgccgac
     8641 acgcaaaccc ttgcggacgg aagcctgttc cgcctctttc gtcatgttgt gcgaccagtc
     8701 cgcaccgccg taaagcagcc agtctttcgg cgcggaatac atcagcgtcg cgcccgccag
     8761 cggcatatgg ctgtcgtatc gggcggcggt gcggtcttcc tgataacgct tccacatatt
     8821 gcccgcgttt agtgtcaacc gccagcgttc gcccaagcgt tgggagaaat cggcattgaa
     8881 gccgccgacg aaattgtatc ggctgccgcc taagaggttt tgcccgacaa acggcacgat
     8941 gccgaacgag cgcgttaccg aacgattttt atagccgagc gacaggtgca ggcactgttc
     9001 gctgaaatct ttgttatccc aataatgcac gccgccgccg ctgatgccgc cgtagaggaa
     9061 atgatgcccg cccgcattga tttcgcgcga cacgcctaag ccctagcgca aaccgtgtgc
     9121 cttttgcggc aggctgtcgg cggttttcgt ccagcttctg cccgcaaatt caatcgtttt
     9181 tttggacgaa gcgttgttta tagcggatta acaaaaatca ggacaaggcg acgaagcctc
     9241 agacagtaca gatagtacgg aaccgattca ct
//
LOCUS       DAWKOD010000072         9132 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000072 DAWKOD010000000
VERSION     DAWKOD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.072"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..1328)
                     /locus_tag="WJK73_001264"
     CDS             complement(72..1328)
                     /locus_tag="WJK73_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ7557743.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1676..2017)
                     /locus_tag="WJK73_001265"
     CDS             complement(1676..2017)
                     /locus_tag="WJK73_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557744.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2221..3078)
                     /locus_tag="WJK73_001266"
     CDS             complement(2221..3078)
                     /locus_tag="WJK73_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ7557745.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3139..3954)
                     /locus_tag="WJK73_001267"
     CDS             complement(3139..3954)
                     /locus_tag="WJK73_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7557746.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4198..4983
                     /locus_tag="WJK73_001268"
     CDS             4198..4983
                     /locus_tag="WJK73_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ7557747.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5242..7364
                     /locus_tag="WJK73_001269"
                     /pseudo
     CDS             5242..7364
                     /locus_tag="WJK73_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            7488..9038
                     /locus_tag="WJK73_001270"
     CDS             7488..9038
                     /locus_tag="WJK73_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ7557748.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVSAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEVDIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2296 a         2170 c         2409 g         2257 t
ORIGIN      
        1 tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta ttgcaaaggt
       61 ctcaaaccgg tttatttacg ttgcaatacg gcggcgaaga agccgtcggt ttggtgtcgg
      121 gcggagttga ggcgcaggta tttgccggta tccaaatcga ttttcaaacc ggcaagcagt
      181 tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc gacttgcagc
      241 tcgttttctt cgggcaggat gctgcaagtg gcgtacacca agcggccttg cggttttacc
      301 agtttggagg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc cacggtttcg
      361 gcggattggc ggtatttgag atcgggatta cggcgtaaag tacccaaacc ggagcagggc
      421 gcatccacta aaacgcggtc ggctttgcct gccagtcggg cgatacggct gtcgtgttcg
      481 ctgctgatgc gttcagggtg gatgttggtc agtccggcgc gggtcatacg cggtttgagg
      541 ttggcaaggc gtttttcggc gatatcgaag gcgtagattc tgcctttgtt cgccatttgc
      601 gcaccgacag ccaaggtttt accgccggca ccggcacaga aatcgacaat gatttcgccg
      661 cgttttgcgc ccaccaataa ggcaagcagc tggctgcctt cgtcttgaac ttccagcgta
      721 ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg gatgccccaa
      781 ggcgaataag gcgttgcctc tgcatcggca ctttcggctt gcaacaatgg cagcacttta
      841 tcgcgtttgc ctttcaaggt attgacgcgt atatcgagcg gcgcagcttg gttgatgctg
      901 cggccgaagg cgaggatttc ttcttcgcgc caatgctgct gcagctgttc taccagccat
      961 tgcggcaatt ctgcggcggt attcaggccg tctgaaaact cggttttacg ggctttcaaa
     1021 ttgccgagaa actctatttc ttcttcatca agcaggtctt tgatttggct gatgttggtg
     1081 cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg cggacggcgc
     1141 agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcggtttc ggcgatttcg
     1201 tggcggtctt ggcggccgag ctttttgtgt tcgcggaaat aggcggagag gacggcatcg
     1261 gaaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc gagttgtgcg
     1321 gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc taacgtcctt
     1381 aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag aaagatgtgt
     1441 taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg cagcctgaaa
     1501 aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag cgatttcatt
     1561 ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc gttcagtata
     1621 tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc tcttgttaaa
     1681 tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca ctctcattgg
     1741 ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt tcatttgtat
     1801 gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca tatatatctt
     1861 cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct tttttcagat
     1921 actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat gattgcttga
     1981 cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg gggtcgtagt
     2041 tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca tgttgttgtt
     2101 atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa ttgtatggga
     2161 aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg ggaaagggtt
     2221 tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc gacattccaa
     2281 aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg tttcggtttc
     2341 ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc ggacttcttg
     2401 gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac cgtatggatt
     2461 aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc gcacgatgga
     2521 aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc cgtctgaagt
     2581 gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg tgtctttggg
     2641 gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt tgcaggtcag
     2701 ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc cttgcgtatt
     2761 gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg gcgcgttggc
     2821 aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga acggggcgcg
     2881 tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa gatagcggaa
     2941 accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc acaaaggcgg
     3001 cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc accataatgc
     3061 gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa agctggaaat
     3121 gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg tattgaaaaa
     3181 accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca ggctctgcat
     3241 acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt tcatcacgct
     3301 ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt cttcttgttg
     3361 aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga cctgcacggc
     3421 ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca gtcggttgca
     3481 ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca gtgccacttt
     3541 ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg tttgcagcgg
     3601 cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt cggcaagggc
     3661 ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc cggaagcgag
     3721 gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa acacgggcgg
     3781 gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt tgtgcagacc
     3841 cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg tgcgcgtgag
     3901 gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag tcatgttcgg
     3961 ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga cggtcaaatg
     4021 ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa gcagacacgc
     4081 gtcaggcggt attttacccg atttggcagg atttacccca accaaataaa ctccgatttt
     4141 gccgctccga aggactgtac gtccgaaccg gcggtatgcc acccttaagg aaatctgatg
     4201 aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat gatgatacgc
     4261 gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag ccttcaacga ttttgtttcc
     4321 gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc ctatccgcac
     4381 cacaaaatca cttgtgaaga aagcggctcc cacggcaaag ccgcagccga gtacgaatgg
     4441 attatcgatc cgctcgacgg cacgaccaat ttccttcacg gtcatcccca atacgccatc
     4501 tctatggcgc tgctgcacaa aggcgtgttg caagaagctt tggtttacgc ccccgaacgc
     4561 aacgacgtat atatggcttc gcgcggcaaa ggcgcgttgc tcaacgaccg ccgcatccgc
     4621 gtttccaacc gcatcgaatt gaaccgctgc ctgatcggta ccggcttccc tgttgtcgat
     4681 caaagcatga tggacaagta tctggtgatt ttgaaagatt tcttggccaa aaccgccggc
     4741 ggccgtcgtg aaggcgcggc ttctttggat ttgtgtgctg tagcaacagg tcgttttgac
     4801 ggctttttcg agtttaacct caaaccgtgg gacattgccg caggtgcatt gattgttcag
     4861 gaagcgggcg gtatcgtaac cgatatgtcg ggtgaagacg gctggctgga aagcggcgat
     4921 attgtggctg ccaatcccaa agtgctggcg caaatgttga aaatcatttc cgcacacgtt
     4981 taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt ctgaagtatc
     5041 gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg gaaacggcta
     5101 aatgcccgaa actgccgagg atggaaacta aacggagaac cctgtgcaat ttgtttatag
     5161 tggattaaca aaaaccagta cggtgttgcc tcgccttagc tcaaagagaa cgattctcta
     5221 aggtgctgaa gcaccaagtg aatgggcaaa gccaacgcct ttaccgcgcc gattaccgtc
     5281 gtcaattacg acgaacaagc cctaaacaac accgaagcgc gtactttggt ggatgccgta
     5341 gcgaaaaaag acgcttccgt ttggcagttc ggcggcgaaa gcaacacgct gaccggactg
     5401 tatttcagag gttatcagct tgactcgcgc caattcagcg tcaacggttt ggcaggtatg
     5461 tacggcacgc aaggcacggc cagcgtgcaa gttggatcgg cacaactgat taaaggcgca
     5521 tccactgccg taaacggcat ggaccctgaa ggtgcggtat ccggttccgt caatatcgaa
     5581 accaaaaaag ccgccgatga aggcaaccgc aaaatcggtt tgggctggtt cagtaacaac
     5641 cgcgcccaag gtacgttcga cttgggccaa cgtttcggcg aaaacaaagc attcggcgtg
     5701 cgcgccaacg gcaaactgcg ccacggcgac accccgcgcc acggttacag cgaagacaac
     5761 aaagaatttg cggtaaatgc cgactatcgc ggcgaaacac tgcgcgtagc gttcgattcc
     5821 atctacgcaa aacgcaaaac caacggcggt cgcgcgcgta tgcaggacat tcaaaacgcc
     5881 ggcggacgct tgtttggcgc gcccgatggt aaaaccaacc tgctgccttc ttggaactgg
     5941 caaaataccg ccggccaaac caatatgctt acgtttgaat gggatgcgtt tgataatgca
     6001 cagattacag gcggtatcgg ctacaacaag gcacgctatt acggcacgct gatttccccg
     6061 accgtatgtt tgaatgctac cagcacttgt accgatatgc aaaacacaaa cgccagcggc
     6121 aaagtaacgg cacgcaacta tgactacaat accggtactg cgcgcctgac cgaccaatat
     6181 ttccgcacat tgagtatgaa cctgaccgca cgcggcgaat ttgaaaccgg tccggtaaca
     6241 cataactgga gcaccgcgtt cgaccgtgtt atccgccagc ggaaaaccat aaacggcagc
     6301 ggaaacggca acagcaagat agaagtgaag gcaaacgaaa atattgcgaa tcaattggca
     6361 tcgttcaaag cctactatcc caattcttgg gcgaaaaccg ccaacttgga tgccaatatc
     6421 aaagttaaca gcctggcttt gtccgacacc ttgggcttcg ttgataacaa ataccgcctg
     6481 actttgggcg gacgtttcca agcggttgaa tacaccgata agaaaaaatc acagagcggc
     6541 gatgccaaac gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt
     6601 gtgtacggca actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc
     6661 gaaacgacaa tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac
     6721 tggggcaatt tcgtaaccac gttaaacgcg ttccaaatca aacgcccggc tactggcgcg
     6781 gtcatacaac taccaagaca acaaaagggg taacaacaac gctgacctat ggcaacaatt
     6841 ccgatttcgc ccgctataag gcgcaaggcg gcgcggccgg cgacgagcaa ggtatggaac
     6901 gcaatcgcgg tattgagttc aacacctacg cgaacttgct caacaatact ttgcgtccga
     6961 ctttgggtct gatgtatctg caatcgaccg tgaaagagta tccaaattca cgcgatatgc
     7021 tggttaacgg cgtacaagtc gccaatccgc gcgtaattgc caaagcaggt ttggaatggg
     7081 atacaccgtt tgccaaagga ttgactttaa acggtaatgt ttcgtatttc ggcaagtctt
     7141 accaagacac gcaaaaacaa tacgccttcc cgtcctacac cttgattgac gtaggcgcgc
     7201 gctacaaaac caagctcggc aaaaatactt tgaccgtcag cagctcggtg gaaaacctgt
     7261 tcaacaaaaa ctattggcag gtacagcgcg gccaatacga ccgcagcttc gccgtcgtcg
     7321 gtatgccgcg cacttactgg ctgaaggcgg aattggattt ctgatttccc tatatccgga
     7381 aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agagatatgg cgggatacgc
     7441 ttaaataatg ctattctagc gtctttgaaa cctgcttgtt tgaagtgatg aaaaaacctt
     7501 tgatttcggt tgcggcagca ttgctcggcg ttgctttggg cacgccttat tatttgggtg
     7561 tcaaagccga agaaagcttg acgcagcagc aaaaaatatt gcaggaagcg ggcttcttga
     7621 ccgtcgaatc gcaccaatat gagcgcggct ggtttacctc tacggaaacg acggtcatcc
     7681 gcttgaaacc ggagttgctg aataatgccc ggaaatacct gccggacaac ctgaaaacag
     7741 tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc gccggcggat
     7801 tcggcacgca ggcgtacatt gaaaccgagt tcaaatacgc gcctgaaacg gaaaaagttt
     7861 tggaacgctt ttttgggaaa caagccccgg tttcccttgc caataccgtt tatttcaacg
     7921 gcagcggcaa gatggaagtc agtgtttccg ccttcgatta tgaagaactg tcgggcatca
     7981 ggctgcattg ggaaggtctg acggggaaaa cggtttacca aaaaggtttc aaaagctacc
     8041 gcaacagcta tgatgccccc ttgtttaaaa tcaagctggc agacaaaggc gatgccgcgt
     8101 ttgaaaaagt gcatttcgat tcggaaactt cagacggcat caatccgctt gctttgggca
     8161 gcagcaatct gaccttggaa aaattctccc tagaatggaa agagggtgtc gattacaacg
     8221 tcaagttaaa cgaactggtc aatcttgtta ccgatttgca gattggcgcg tttatcaatc
     8281 ccaacggcag catcgcacct tccaaaatcg aagtcggcaa actggctttt tcaaccaaga
     8341 ccggggaatc aggcgcgttt atcaacagtg aagggcagtt ccgttttggc acactggttt
     8401 acggcgatga aaaatacggc ccgctggata ttcatatcgc tgccgaacac ctcgatgctt
     8461 ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa atgactgagg
     8521 aacaaatccg caatgatttg attgccgccg tcaaaggcga ggcttctgga ctgttcacca
     8581 acaatcccgt attggacatt aaaacttttc gtttcacgct gccgtcagga aaaatcgatg
     8641 tgggcggaaa aatcatgttt aaagatttga agaaggaaga tttgaaccaa ttgggtttga
     8701 tgctgaagaa aaccgaggta gacatcagaa tgagtattcc tcaaaaaatg ttggaagact
     8761 tggcggtcag tcaggcaggc aatattttca gcgtcaatgc cgaagatgag gcggaaggca
     8821 gggcaagtct tgacgacatc aacgagacct tgcgcctgat ggtggacagt acggttcaga
     8881 gtatggcaag ggaaaaatat ctgactttga acggcgacca gattgatact gccatttctc
     8941 tgaaaaacaa tcagttgaaa ttgaacggta aaacgttgca aaacgaaccg gagccggatt
     9001 ttgatgaagg cggtatggtt tcagagccgc agcagtaatg ggtcaaatgc cgtctgaaat
     9061 gttcaggcgg cattttgttg tattggaaac tgccgttttc aaccattcct ttagataagc
     9121 cgtgtcggat tc
//
LOCUS       DAWKOD010000073         8976 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000073 DAWKOD010000000
VERSION     DAWKOD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.073"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1467
                     /locus_tag="WJK73_001271"
     CDS             <1..1467
                     /locus_tag="WJK73_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7557749.1"
                     /translation="TNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSG
                     LVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKV
                     LGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAGNDYLEGGSGS
                     DTYVFGKGFGQDTVYNYDYATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQV
                     TVQSYFQNDGSGAYRIDEIHFDNGKVLDVATVKKLVQQSTDGSDRLYAYQSGNTLNGG
                     LGDDYLYGADGDDLLNGDAGNDSIYSGNGNDTLDGGEGNDALYGYNGNDVLNGGEGND
                     HLNGEDGNDTLIGGAGNDYLEGGSGSDTYVFGEGFGQDTVYNYHVDKNSDTMHFKGFK
                     AADVHFIRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAG
                     NNLVQSMSVFGSNTAATGGNVDANIQSVQQPLLVTPSA"
     gene            complement(1809..4412)
                     /gene="pepN"
                     /locus_tag="WJK73_001272"
     CDS             complement(1809..4412)
                     /gene="pepN"
                     /locus_tag="WJK73_001272"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ7557750.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(4548..5342)
                     /locus_tag="WJK73_001273"
     CDS             complement(4548..5342)
                     /locus_tag="WJK73_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7557751.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKIHIGTIGEADDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(6092..6961)
                     /locus_tag="WJK73_001274"
     CDS             complement(6092..6961)
                     /locus_tag="WJK73_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ7557752.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAVLANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(7012..7548)
                     /gene="ruvC"
                     /locus_tag="WJK73_001275"
     CDS             complement(7012..7548)
                     /gene="ruvC"
                     /locus_tag="WJK73_001275"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ7557753.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASRLNPSGMQVKRGRFQ"
     gene            complement(7551..7790)
                     /locus_tag="WJK73_001276"
     CDS             complement(7551..7790)
                     /locus_tag="WJK73_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ7557754.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(7820..8830)
                     /gene="dusB"
                     /locus_tag="WJK73_001277"
     CDS             complement(7820..8830)
                     /gene="dusB"
                     /locus_tag="WJK73_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ7557755.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         2099 a         2271 c         2320 g         2286 t
ORIGIN      
        1 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt attccaaacc
       61 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      121 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      181 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      241 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      301 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      361 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg tttctttata tggtaatgac
      421 ggcaacgaca ctctaatcgg cggtgccggt aatgattact tggagggcgg cagcggttcg
      481 gatacttatg tcttcggcaa aggcttcggt caggatacgg tctataatta cgactacgct
      541 accggacgca aagacatcat ccgctttacc gacggtatta cagccgatat gctgactttt
      601 acccgagagg gcaaccatct tcttatcaag gcaaaagacg gcagtggaca agtgactgtt
      661 cagtcctatt tccagaacga tggctcaggt gcttaccgta tcgatgagat tcatttcgat
      721 aacggcaaag tactggatgt tgccactgtc aaaaaactgg tacagcaatc caccgacggt
      781 tcggacagat tgtatgccta ccaatccgga aataccttaa atggcggatt gggcgatgac
      841 tatctgtacg gtgccgacgg ggatgacctg ctgaatggtg atgcaggcaa cgacagtatc
      901 tacagtggca atggcaatga tacgctcgat ggaggagaag gcaacgacgc cctgtacggc
      961 tataatggta acgatgtact gaatggtggc gaaggcaatg atcatttgaa cggcgaagac
     1021 ggtaacgaca ctctgatcgg cggtgccggt aatgattact tggagggcgg cagcggttcg
     1081 gatacttatg tcttcggcga aggcttcggt caggatacgg tctataatta ccatgtggat
     1141 aaaaactctg acactatgca ctttaaagga tttaaagcag cagatgttca ttttatccgt
     1201 tccggaagtg atttggtgct tagcgcttct gaacaagaca acgtacgtat ttccggattc
     1261 ttctatggtg aaaaccatcg tgtagataca tttgtctttg atgatgcagc tatcagtaat
     1321 ccagattttg ccaagtatat taatgctggc aataatttgg tacagtctat gtctgtgttc
     1381 ggttctaata ctgctgcgac aggaggaaat gtggatgcca atatacaatc cgtacagcag
     1441 ccgttattgg taacgccatc tgcataagga gcctaattac attcatggct taaactgaaa
     1501 aacagcaatc aagtttattt tgattgctgt ttttcttaat attgggataa gggtcgtatt
     1561 ttaattaacc ttaatcggtg cacttctagc aatatagtgg attcacaaaa accagtacag
     1621 cgttgcctcg ccttaccgta ctatctgtac tgtctgcggc tttgtcgcct tgtcctgatt
     1681 tttgttaatc cactataatt aatatgactt tgcggccgtt ttgccattgc gtaataaaac
     1741 gatggggaag tgatgataaa acgtgtgtgt aactatatca gacggcattg tttttctgtt
     1801 tgacggcctc aatccaaaat tttgccgacg atttcgccca cgtctttcga caatccttcc
     1861 tgcgcccgaa tgcgctgcaa tgcttgtttc accaagtttt tgcggtgcgg ttcgagctta
     1921 ttgcagagat tgaacgcctg cactaggcgg gcggcgacct gcgggttaaa gcggtcgatt
     1981 tcgatgactt tgtcggcgat gaagcggtag ccgctgccgt cttctgcgtg aaaatgcggt
     2041 acgttgcggc tgaagctgcc gatgagcgag cgggctttgt tggggttttc gaggctgaac
     2101 ttcggatgct gcaaggcggt ttgaacctgt tgcagggtgt cgctgcggcg gcttgatcca
     2161 acgagggcga aatatttgtc catcaccagc gcgtcgtctg aaaacttgtc ggcaaactga
     2221 gccagcaggc ggttgcgcgt atcgctttcg ttgccgttaa cggcggacag gatgccccat
     2281 tcgtgggtca tgttttgcgc catttcgccg tatttttcgg caacggtttc gatgtgcgcg
     2341 gggtcggcgc acaggacgaa ggcgcggcag acgttgcgca gcgtgcgcca gccggcagct
     2401 tcggggctgt actcgtagct ttggttttcc tgcttcgccg cctgacggtt caattcgtgc
     2461 catttcggca ggaagcggac ggcgattgta tccaacaagg cttcgtgcgc ctgatggtag
     2521 cgcaacgggt cgatgttttc cgtgccgtcc cacagctcgg cttcagacgg cacgcccaaa
     2581 agcagggctt tgaaggcgtt gtctaagagg tcgtctgaaa tgactttttc gacggcggca
     2641 agcagttttt cgtgtttcgg cagttcaata ccgtctgaaa gcgcggcaag gttggcggcg
     2701 acggcgcggc ggtagagcgt ttgggcggct tcccagcgcg tgaaggcgtc gctgtcgtgg
     2761 gcgagcagga gcagcaggtc gtcgttgctg tacggatagt tcagatgcac tggcgcgctg
     2821 aacccgcgca gcagcgaggg aacgacggct tcggttacgc tttcgaactg gaaggtctgt
     2881 tcggcttcgg tcagcagtaa cacggcttcg gttgcgcgtt tgccctgata atcgaatgcc
     2941 accgcttcgc cgttgcggtt cagcagcccg actttgacgg gaatcatcat cggctgcttg
     3001 tccgccatat cgggcgtggg cggcacggtt tgtttgatgg ttaactcgaa actattgttt
     3061 ttcagacgac cttccgcttc caagacgggc gtacccgcct ggctgtacca caaggcgaac
     3121 tggtcgagat tgatgccgtt cgcgtccgcc atcgccgcgc ggaaatcgtc gcaggtcacg
     3181 gcctgcccgt cgtgtcgttg gaaatagagc ttcatgcctt tttggaagcc ctcttcgccg
     3241 agcaaggtgt gatacatccg caccacttcc gcgccttttt cataaacggt catggtgtag
     3301 aaattgttca tctcctcata gctggcgggg cgcaccggat gggcggtcgg gcctgcgtct
     3361 tcggggaact ggtgctggcg cagcaggcgg atgttctcga tgcggcgcac ggcgcggctg
     3421 gcgcggtcgc cggaaaattc ttggtcgcgg aacacggtca acccttcttt cagcgaaagc
     3481 tggaaccaat cgcggcaggt aacgcggttg cctgtccagt tgtggaaata ctcgtgtccg
     3541 accacggatt cgatgccttc aaaatcggta tcggtggcgg tacggctgtc ggcaaggacg
     3601 aacttggtat taaaaatgtt cagccccttg ttttccatcg cgcccatatt gaaatcgccc
     3661 acggcgacga ccatgaaaat atccaagtcg tattccaaac cgaagcgcgt ttcgtcccac
     3721 ttcattgcgt ttttcagcga ttccacggca aagccgacct tgggcttgtc cgcttcggtg
     3781 gtgtaaaact cgattttgac gtttctgccg ctcatggtgg tgaaatagtc ttccgtgacc
     3841 gccaaatcgc ccgcgaccaa agcaaacaga taactcggtt tggcaaacgg gtcttcccat
     3901 ttcacccaat ggcggccgtc tgaatactcg ccgccgtcga ttttgttgcc gttggagagc
     3961 aaaacgggat agcgtttttt gtccgcgacg atggtggtcg tgaacttgga catgacatcc
     4021 ggacggtcga tatagaacgt gattttgcgg aagccctccg gctcgcactg ggtaaacagg
     4081 ttaccggcgg acgcatacag ccccatcagc gatttgtttt ccgccggcag gatttcggtt
     4141 tccacttcga cggtgaagcg ttcggacggc acgtctgcaa tcgtcagcgt ctcgccttcc
     4201 aacatataat ccgccgccgc gccgttgatt ttgacggaca agagtttcgc cgaaccgtcc
     4261 aacaccagcg gctcccctac cctctgcggc tcaaccgtca aacgcgactt cacgacggtt
     4321 tgcggttcgg caatatcaaa atgcaggtcg gtttcaagga tgcggtaggc aggcgtttgg
     4381 taatctttca gataacggac ggttttgctc atttttttct ttcaatattg tttggtttga
     4441 ctggaaaagg cttcagacgg cataggcaaa ccctgcaccg atatgccgta tgaaaccgaa
     4501 acatcagtgc ggacgcaccg tcaaacaacc ggttttaatt tcaatcttta tttccacgct
     4561 tggacaaatt ctgcccaatg cggtttgtct tctgtttggg cggaaagata gtttctgatc
     4621 cgcttgattt ccatttcgta ttcgtccgcg tcgaatctgc cgctgaccag acagaaacgc
     4681 agatacatca aatacgtatt cacggcatcg gtttcacaat aattgcggat ttccttcagc
     4741 ctgcccgtat ggaatgcctc ccaaaccttg ctgccgtcca tacccagctt gcccggaaaa
     4801 ccgcacagtt tcgccatatc gtccagcggc acgtttgccc tcggctgata aagtgcgagc
     4861 aaatccatca aatcgcagtg gcgttggtgg taacggctga tgtagttgtt ccacttgaaa
     4921 tcgcggctat cgccgaaatc gccgtcgccc atatcccaat agcgcgcggc gttgatgccg
     4981 tatatcaggg agcggtaatg cagtacgggc aggtcgaaac cgccgccgtt ccaactgacc
     5041 aattgcggcg tatgtttttc caccaactcg aaaaatttgg cgataaccac ttcctcgccg
     5101 tcgtccgcct cgccgattgt gccgatatgg attttgtcct gcccccaacg catgcagcac
     5161 gaaatcgcca caacctgatg aagatgatgc tgcataaaat cgccgcccgt ttgggcgcgg
     5221 cgtttttgct gggcaaacag caccacttcg tcgtcgggca gcgaggacgg cagctcgtac
     5281 aatgttcgga taccctggac atcaggtacg gtttcaatat cgaaagccaa aatcgtattc
     5341 atggcagtac cttgcattca aaaacagact tgcgcctatt gtgtcattaa aagacagata
     5401 aaaaaagagg caacccccca caggattgcc ccaatacctc aaatcagaga tttacgcttc
     5461 acaaacaata caggctttcg cctgcggctt tacccgcgta gctcaactct acgccggcaa
     5521 actttcgttt caccgtttcc gatgaaaccc cgaccaatcg caagactgac cggaaaatcc
     5581 tttcaaacag catttcctgc ctgtcgtgta attccatgta gcgaaatgta cgccattttc
     5641 tacgctttgc caagcatttt ttacaatata aatgtcaaaa cattaatttt ataaaattgc
     5701 tgaaaatatt aaatatatgg atttttattt tttatatttc aataaatata aatttaattt
     5761 tgatttatat ttaaatttaa gcataaaatg tcaaatatta aaggaaacat gaaaggcata
     5821 tattaaatat ttatttataa ctctatgttt ttaaagaaaa ttaattttaa tatattaact
     5881 agattgtctg catatattca taggtttgcg gtatttcttc caaaacctgc ttcgaatttc
     5941 ccgaccaagt cttaaaaata ttgtttttga gatacttaaa tagcagcgat tatcaaatga
     6001 aatctgttca tataatctgc cattttgcat ttaaaaaaac aatcaggagt ttcgactcga
     6061 aatgcctgat atgttttgta attttacgta gtcagtaaaa atcggggctg ccttccggac
     6121 gggttttaaa acgcttgtgc agccaaaaat actgctcggg atgttcgcgc acgcgttcct
     6181 cgataaaacg gttcatgcgc tgcgcatcgg cttgcgcgtc ttcacccgga aaggatttcc
     6241 aagccgggta gaaatgcaat gtaaccgtat tgtccgcctc cctgacgggg atggcgggaa
     6301 tcactttggc atttgcaagc acggcaatgc ggctcaatcc ggtaatcgtt gccgtccgaa
     6361 taccgaaaaa atccacaaaa accgaatcgt tgcgtccgaa atcctgatcg ggcagataca
     6421 gaaacggcgc gctgcttttg cggaactgtt tgacgagggc gcgcagccct tcggtgcgcc
     6481 cgatgaggaa gacattgtcg tagcggttgc ggcctttcaa aatctgtgcg tccaatatct
     6541 tgtttttttg gtgggaatac atactaatta gcgggacatc ctgattaagc gcgtacaccg
     6601 ccatctcgaa cgcggtaaag tgcggataca ggatgatgac tttttccccc gccgccagcg
     6661 cgtcgtccaa ataatgctta ttgcggtagc gcaccagcga tttcaaacgc ccggcgggcg
     6721 cgtaccaata taagccgtat tcgagcatca gtttcgccat atgtttgaaa tgctgtttca
     6781 acacggtttt acgcttttcc tcactccatt cgggaaaaca tttcgccaaa ttgatttcgc
     6841 cgatacggcg gcgcggtttg accagaaggt aggcaagcaa acccgtcagg tcggcaatct
     6901 tgtgcagcag cgcaaacggc agaaactgca aaacatacag tacaaaaaat ataaatttca
     6961 tctcgataca cattttcttt tcagacggca aaatacaaat gccgtctgaa actattgaaa
     7021 ccttccgcgc ttgacctgca tccccgacgg gttgagccgg gacgcaagcc cgtggttgcg
     7081 taaggcgtgg gtcagcgcga cggcaagacc gtccgccgca tccgcctgcg gcgttcccga
     7141 aagccccagc atctgcacca ccatatgctg cacctgttct tttgccgcct tgcctttgcc
     7201 gactaccgcc tgtttgacct gcaaggccgt gtattccgaa acgggcagct tatggctgac
     7261 caatgccgcc aatgccgcgc cccgcgcctg accgagcatc agcgtcgatg caggattgac
     7321 gttgacgaac acctgttcca ccgccgcctg ctgcggcttg taaacggcaa cgacttcgcc
     7381 gatgtgccgc acaatcacgg caatcctgtc tgccagaggc gcatcggcag gcgttttgat
     7441 gcagccggag gcgacgtaaa aatgatcgcg ccccctgaca tcgatgacac cgaaacccgt
     7501 tacgcgactg cccgggtcaa tacccaagat acggacggtt gccgacatat tcacagcaaa
     7561 ccgtgttgaa tcagcttctt acgcagggta ttgcggttca gtcccaacat gacggaggct
     7621 ttggactggt tgccgccgca ttgttccatc acgcacacca gcagcggttt ttccacctga
     7681 tgcaatacca tatcgtacac gccgcaaggt tcggtaccgt tcaggtcttt gaaatattgt
     7741 tccaaatttt gtctgataca ttgggaaata tcgggaaggg tatggggcat gattgcactt
     7801 tcaaaggata atcaagtgtt cagaaggcat ttgggcggta ggcgcacgcc caactgtcgg
     7861 ttttttcggc aagtctttca agataacctg caagcatgtc gtattgcgcc gccgcactgt
     7921 ccaagcggtt gatttcacga cgtgtctgtt cgccgtcggg catttcgtcg atgtaccagc
     7981 ctatgtgttt gcgtgcgatg cgcacaccgg cggtgtcgcc gtaaaacgcg tgtatggcgc
     8041 ggatgtggtt caaaatagcg gcggcgcatt ctgccaaact caaggcaggc ggcaaaacac
     8101 cgtgttcggc ataatgtttc aaatcgcgga agaaccacgg cctgccttgc gcgccgcgcc
     8161 ctatcataat gccgtcggcg gcggtttgtt tgaggacggc ttgggctttt tgcggcgaag
     8221 taatgtcgcc gttgacccag accgggatgt tcagacggca tttggtttcg gcgatgagtt
     8281 cgtaacgtgc ttcgcctttg tacatttgcg tacgcgtgcg tccgtggacg gcaagggcgg
     8341 cgatgccgca atcttcggcg attttggcga taacgggcag gtttttgtgg tcgtcgtgcc
     8401 agcccaaacg ggttttgagg gtaacgggta cgcccgccgc tttgaccacc gcctccaaaa
     8461 tggcggcaac cagcggctcg ttctgcatca gcgcgctgcc ggcttggacg ttgcacactt
     8521 tcttggcggg acagcccata ttgatgtcga taacctgcgc cccaaggctg acgttgtaac
     8581 gcgcggcatc cgccatctgc tgcggatcgc tgccggcaat ctgcacggca acgatgccgc
     8641 cttcatcgac aaaatcactg cggtgcaagg tttttctagt atttctgagc gtcgggtcgc
     8701 tggtcagcat ttcgcacacc gcccaacccg cgccgaaatc gcggcagagg cggcggaagg
     8761 gtttgtcggt aatgcccgcc atcggtgcaa gtgcgatggg gttgtcgata aaatagccgc
     8821 cgatgtgcat aatgggcccg cgtttcaaaa aagtacgcca ttgtacattt tttaagcagg
     8881 atttccaatc tccggacgcg cccgcgattg gggcagacac cgttttatgg cataatccgc
     8941 acacagattc cctgccccgc cactcacagg agggca
//
LOCUS       DAWKOD010000074         8694 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000074 DAWKOD010000000
VERSION     DAWKOD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.074"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(171..863)
                     /locus_tag="WJK73_001278"
     CDS             complement(171..863)
                     /locus_tag="WJK73_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7557756.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WJK73_001279"
     CDS             complement(971..2164)
                     /locus_tag="WJK73_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7557757.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJK73_001280"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJK73_001280"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ7557758.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WJK73_001281"
     CDS             complement(3017..3946)
                     /locus_tag="WJK73_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ7557759.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJK73_001282"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJK73_001282"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ7557760.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WJK73_001283"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WJK73_001283"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ7557761.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7216..7674
                     /locus_tag="WJK73_001284"
     CDS             7216..7674
                     /locus_tag="WJK73_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7557762.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(7945..8160)
                     /locus_tag="WJK73_001285"
     CDS             complement(7945..8160)
                     /locus_tag="WJK73_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557763.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(8168..8371)
                     /locus_tag="WJK73_001286"
     CDS             complement(8168..8371)
                     /locus_tag="WJK73_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557764.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WJK73_001287"
     CDS             complement(8284..8643)
                     /locus_tag="WJK73_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7557765.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2166 a         2412 c         2161 g         1955 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg
     4921 cttttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc
     5101 ggtttgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc
     5521 ggcgacgacg accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg
     5581 ctgatggaag agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc
     6001 ctcttgcgcg tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt
     6061 cagacggcct caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca
     6121 aaagccacca aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac
     6241 tacaaaaccc aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca
     6361 gacgaccccg cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc
     6481 ttggaattta acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agtctcgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt
     6961 gcgaaattgg aagcgatgta gaagggagat ggcagaggcc gtctgaaaac gggaacaaca
     7021 atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 gggaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactacaaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 aaagatcatg tgcaaatttg cgaggtatcg ggaatcggtt ttgaaaaaga aggaagcctg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg agtatccgcc catcggtgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccctatttg ccttagacta tcgggagtgt
     7621 ggaagagacg gagagccgaa agtggtgttt gtcgaacaag aatcggattt ttaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatt cacagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accagaatca tcaaaggaaa acgggcggaa
     7801 taacagataa ccgattgaat ccgtaaggaa aacggtttgt aggaacgtgc catccaagac
     7861 ctttgcaaag aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt
     7921 ggagctatct caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat
     7981 taaatgttgt tctagctctt gcactgattg aggattgcga attcctgata caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata aatagggaaa ttcaaattaa gttagaatta tccc
//
LOCUS       DAWKOD010000075         8691 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000075 DAWKOD010000000
VERSION     DAWKOD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.075"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(334..1425)
                     /gene="ychF"
                     /locus_tag="WJK73_001288"
     CDS             complement(334..1425)
                     /gene="ychF"
                     /locus_tag="WJK73_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ7557766.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLSHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1539..3215)
                     /locus_tag="WJK73_001289"
     CDS             complement(1539..3215)
                     /locus_tag="WJK73_001289"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ7557767.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVAINRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIENQTNNFGFAYDVELSVKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            3390..3821
                     /locus_tag="WJK73_001290"
     CDS             3390..3821
                     /locus_tag="WJK73_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7557768.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4066..4761)
                     /locus_tag="WJK73_001291"
     CDS             complement(4066..4761)
                     /locus_tag="WJK73_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ7557769.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4779..5279)
                     /gene="hpaC"
                     /locus_tag="WJK73_001292"
     CDS             complement(4779..5279)
                     /gene="hpaC"
                     /locus_tag="WJK73_001292"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ7557770.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRSLD"
     gene            complement(5394..5834)
                     /gene="nadR"
                     /locus_tag="WJK73_001293"
     CDS             complement(5394..5834)
                     /gene="nadR"
                     /locus_tag="WJK73_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ7557771.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6177..6686)
                     /locus_tag="WJK73_001294"
     CDS             complement(6177..6686)
                     /locus_tag="WJK73_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ7557772.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(6765..6840)
                     /locus_tag="WJK73_001295"
     tRNA            complement(6765..6840)
                     /locus_tag="WJK73_001295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6805..6807),aa:Lys,seq:ttt)
     gene            7051..8130
                     /gene="apbC"
                     /locus_tag="WJK73_001296"
     CDS             7051..8130
                     /gene="apbC"
                     /locus_tag="WJK73_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ7557773.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         2041 a         2505 c         2197 g         1948 t
ORIGIN      
        1 aagttaatcc actatactcg aaacgcggcg gcgcaaatgc cgtctgaaag gtcattttgt
       61 acgggatatt cggaatgccg gacggctttt tgcaacagcg gggcaggcgg tttgttttgc
      121 aggaatcggg gagggcaaat cggaaatgcg ggtgggattt tatagtggat taacaaaaac
      181 cggtacagcg ttgccccgcc ttgtcctgat ttttgttaat ccgctatatt ttgatacggc
      241 tgcattccgg cggtatggaa acgccgaaaa ccaccaaaac cggcttcaga cggcatttct
      301 ggcaaagccg tctgaaaacc tgccgcattt gggttacacg ttaaacaaaa agtgcatcac
      361 atccccatcc tgcacgacgt attccttgcc ttccacacgc attttgccgg cttctttggc
      421 tttggcttcg ccgccgagcg agacaaagtc gtcgtaagaa atgacttggg cgcggatgaa
      481 gccgcgctca aagtcggtgt gaatcacgcc ggcggcttgc ggggcggtgt cgcctttgtg
      541 tatcgtccaa gcgcggactt ctttcacgcc ggcggtaaag taggtttgca gccccaaaag
      601 gtcgtagccg gcgcggatca ggcggttcag gcccggttct tccaagccca tttcggcgag
      661 gaactcggct ttttcgtcgt cttccaattc ggcgatttcg ctttccattg cggcgcaaac
      721 ggcgacgacg ggggcgtttt cttttgccgc caattctttc aggcgttcga ggtgcggatt
      781 gttttcaaaa ccgtcttcgg cgacgttgcc cacatacatc gccggtttgg cggtcagcag
      841 gaacagcggt ttgagcatcg cgcgttcttc cgcgtccaaa ccgaaggaac gcacgggttt
      901 gccttcgtcc agatgcgaca gcagtttttt gcacaaatcg accagctttt gcgcgtcttt
      961 gtcgcctgaa cgggcgcgtt tttcttcgcg gacgatggct ttttccacac ttgccaagtc
     1021 ggcaagcgcc aactctgtac cgatggtttc gatgtcggca atcggatcga cgcggcctgc
     1081 aacgtggacg atgttgtcgt cgtcaaagca gcgtacgacg ttaacaatcg cgtcagtctc
     1141 gcggatgttg gcaaggaact ggttgcccag accttcgcct ttgctcgcgc ctgcaaccaa
     1201 accggcgata tcgacaaatt cgacgatggc aggctgcatt ttttgcggat tgacgatttt
     1261 tgccaattca gccatacgcg ggtcgggcac ttcgacgatg ccgacgttgg gttcgatggt
     1321 acagaaagga tagtttgccg cttcgatacc cgactgggtc agcgcgttaa agagggtgga
     1381 tttgccgacg ttgggcaaac cgacgatgcc gcatttcaaa ctcatgtttt ttcctgaaaa
     1441 tagagaaatt taacggcgga ttatagcata ccgccgcccg cgttccgaaa aaatgccgtc
     1501 tgaaacggct tcagacggca tccggtttca aaaaccgttc agaacaagcc gtgaatcacg
     1561 ccgtgttcgt ccacatcgat tttctcggcg gccggaactt tcggcagacc cggcatcttc
     1621 atcatattgc cgcacagcgc gacgatgaag cccgcgccgg cggaaacggt gatgccgcgc
     1681 acggcgatgc ggaagccttc ggggcagccc aaaagtttgg cgttgtcgct caatgaatat
     1741 tgggttttcg ccatacagat cggcattttg tccaaaccca gtttttccag cgaagcgatt
     1801 tcggcggacg cttccgcgct gaaatcgaca tcttccgcgc cgtacacttt ttgggcaatc
     1861 gcacggattt tgtctttgac ggacaactcg acatcgtagg cgaaaccgaa gttattggtt
     1921 tgattttcaa tggcattgac gactttgcgc gccaaatccg cgccgcccgc gccgcctttg
     1981 ccccatactt cagtcaggga aacttcaacg ccgtgttccg cgcaggcttt ttcaatcatg
     2041 gccaactcgg catcggagtc ggacacaaag cgattgatgg caaccaccac aggcagtccg
     2101 aatacgtttt tcaggttgga aatgtgtttc agcaggttgg gcaaaccttt tgccaaggct
     2161 tcgaggtttt cttcgccgag gttggcacgc tccacgccgc cgttatattt caaagcgcgg
     2221 acggtggcca cgacaacagc cgcatcaggt ttcaaatccg ccagacggca tttgatgtcg
     2281 cagaattttt ccgcgcccaa atccgcgccg aagcctgctt cggtaacggc gtaatcggca
     2341 aggtgtttcg ccagacgggt tgcggttacg gagttgcagc cgtgggcgat gttggcgaac
     2401 gggccgccgt gtacgaaggc gggcgtgcct tcgatggttt gcaccaagtt gggcttaatc
     2461 gcatctttaa gcaatgccgc catcgcgccg tgtgccttca aatctttggc ataaacgggg
     2521 ctgccgtctt tggcgtaggc gacaaggatg ttgcccaaac gctctttcaa atcgctgatg
     2581 tctttggcaa gacagaatac cgccatcact tcggaagcaa cggtaatgtc gaaaccgtca
     2641 ggacgcatca cgccgtcaac aggtttgccc atgccgtcga tgatgttgcg caactggcgg
     2701 tcgttcatat cgaccacgcg ccgccacagc acgcgtttcg gatcgatgtt caactcgttg
     2761 ccttggtaaa tatggttgtc aagcatggcg gcgagcaggt tattcgccgc accgatggcg
     2821 tgaaaatctc cggtgaagtg caggttgatg tcttccatcg gcaaaacttg ggcatagccg
     2881 ccgcctgccg cgccgccttt cacgccgaac accggcccca gagaaggttc gcgcagggca
     2941 atcacggcat ctttgccgat gtggcgcaac gcgtccgcca aaccgatggt tacggtggtt
     3001 ttgccttcgc ccgccggagt cgggttgatg gcggtaacca aaatcagcct gccctgtttt
     3061 tgcggcagtt tgaacgcttc ggcaggattg attttcgcct tgtaatgacc gtaaggctca
     3121 atgttgtcgg cattcagacc aagcttggcg gcaatttcgc caatcgggcg catggtggag
     3181 gattgggcga tttcggcatc ggttttgaag ctcatgattt tcctttagaa atgaggaggg
     3241 acatgccgtc tgaaagcatc aggcgacaaa caggtggatt gaaaataata tcaggcatat
     3301 tataacgtta tccgcaccaa acccgcagtg aaatttttga cgctgcaaca aaaataccgt
     3361 tcatattgtt cacaatccaa ggagaaaaca tgagcagcaa cgcatggctg ttttgggcat
     3421 tggcatcggc aggcttcgcc tcattgaccg ccatcttcgc caaaatgggt ttacagggta
     3481 tagattcaga tttcgctacc ttcatccgca ccttggtcat cctcgccgct ttgttattgt
     3541 ttttaaccta caccggcaaa tggcagggcg tgaacggctt tacggggcac aactggacat
     3601 tccttatcct atccggcctt gctaccggcg catcttggct tgcctacttc aaagccctgc
     3661 aactgggtaa cgcctcgcaa gtcgcccccg tcgacaaatt cagcctggtc ttggtcgcgc
     3721 tgatggcggt ggttttcttg gacgaacgcc cgaacacgca ggaatggata ggcttggggc
     3781 tggtaacggc gggcgtgttg gtgctggcgt tgaaacgtta aaccgaatcc gccataccgc
     3841 ctgaaaccgg gttttgacaa tctgacgggc ggcggtctga acaaagttac ccaataaaat
     3901 ccgacaccgc gccgacggca aaccgccgca tccgacaggg tcggcttcag atggcatttg
     3961 ttggacggaa agccccgaac gccctatttt cacgcctcca cgaaaatatg ccggacatcc
     4021 ggtcggaacc gttcggcagc cgactcggca agccaccgtt ttatttcact tccaagcccc
     4081 tgcaagggct tgagcctctt tcagacggca taccgtgccg acatccagcc acaagcccgt
     4141 atgcttctgt ccgctcacgc ggttttgccg catttcgccc cgcaatacgg gcgcgagttt
     4201 cgccacactg cccgcttcga ttccgtcaaa catttcagga cggtaaatac ccacgccgct
     4261 gaatgtcaat ccgttgccgc cggatacttc cgactgtacg cttccgtccg gcagcaggga
     4321 aaaatcgccg tcagggttgt gcggcggatt ttccaccagc cacagatggg cggaaatatg
     4381 ttccggcagg gacgatgccg tctgaaacgc ggcggtaaaa tcgatgtcgg tcagcacgtc
     4441 gccattgacg accaaaaacg gggtatcgcc caacagcggc aatgcccgtg cgataccgcc
     4501 tgcggtttcc aagccgcctt caggttcggg cgaataggcg atgttcacgc cataagccga
     4561 gccgtcgccc aaagcatctt ctatctgccg acccagccaa gcgtggttga tgacgatttc
     4621 ggtaaacccc gcctgcttca gacggcatag gtgccaaccg attagaggct tacccgccac
     4681 atcgagcagc ggcttcggag tggtatcggt caaaggacgc atacgctcgc cgcgtcctgc
     4741 cgccagtatc atcgctttca tatatatctg tccgaatatc agtctaaaga tctaaactgc
     4801 cgtctgaaat aaagcagcgc ggggcgtttg cacccgcagt ttttgatttc gtcgagcctg
     4861 acgtaaaaca caaaatgcgt gccgatttca tgtttgccga caatatgccc gtgcaggtgc
     4921 gccaacgcgc cttctatttc aagttgtccc gttttgccgc gatgccagat gtggtaggca
     4981 aaccgctctt cgggcgacag gccggtcagc ccggcaaaat gttcggcaac atcctgatgt
     5041 tcgtccgcca gcgtattgat gcagaggctg ccgttttccg acaggatcgg aatgattcgc
     5101 gcactccggt tgatgcacag catcacggtc ggcggctcgt ccgtaaccgg cgcaaccgcc
     5161 gtcattgtaa tgccgtaacg ccctgccgca ccgtctgtcg tgatgacatg aacgcctgcc
     5221 gcacaggatg ccatcgcatc acggaacgaa gtttgaaaag ttttctgcaa atccgccatt
     5281 tttccccttt aaactgtccc ctatataaga atgctgcaca caaggcaccc cccatgtgca
     5341 gcagttctga ttcaaaaagc cgtcggtcgg acatttccgc gcgttacggc gtattacgag
     5401 ttcaacgcat cctcgatttt ggcaagttct gccaacaggt ctttaagcag cagcattttc
     5461 tcgcggccca gcacttcctc gatagcgtcg taacgctcgt ccacttcttc gccgatttcc
     5521 tcatacagct tctcgccctc ggcagtcagc ttcagaaaaa cgcggcgttg gtcgttggaa
     5581 ggtttcaggc ggacaaccaa acccgctttt tcaaggcggg tcaggatacc ggtcaggctg
     5641 gggcgcaaaa tgcacgcctg attcgccaaa tcttgaaagt ccagcgtgcc gttttccgcc
     5701 aaaagacgga taatccgcca ttgctgatcg gtaatattcg cctgattcag aataggcctg
     5761 aattgggtca tcagggcttc ccttgcctgt atcagactga tattgataga cgcatgtttt
     5821 gattgggtag gcattgttca agtctccaag ttatcgaaaa tcaaactttc aaaccgtcgg
     5881 gaaagcctgt gggcgtaaat tttgatgcaa ccgttatata acaaaacgaa catatagcaa
     5941 caatacgcta taaaccgcat cggacgactg ggtataaaag actttaattc cgataatcct
     6001 atctaaaaat attttaatag ttatatctta gtctattttc cctacaatct caacaaagta
     6061 ttacatttac tgtcgaaaga actttcttcc agaaaaaaca aaagccgccg catcagacga
     6121 gcaaggtata tgccgtttga ttctctacat agcgtaaagt ttaacaaaaa caaaagttaa
     6181 ccgaaaacat ccgcctgaaa aattcgtgcg cgcaagcccc aataactgct gattcccgtc
     6241 gtatagtgaa ccattttccc atttttgacc aaaacgacgg caggcgttgc gacaatccgc
     6301 caagaccttg ccaaaccccc gtcctcatcg ttgacagtcg gaaagcccaa gccgcgtttt
     6361 gccatatacg ccgccacttc cgccgaactg ccggaacgta ccgccacgcc gacgaccggc
     6421 acgccgtccg ccgccaaatc atcgattatc ggcgactgat aacggcacac gccgcaccag
     6481 cttccccaaa aatacaccag caccgtccta tcccggctaa actgtcccaa agtcagccgc
     6541 tgccccgaca gcagggtcaa aggccgccct gccgcgccgg caggttcgtc gggcttgcgt
     6601 acccaatcca aaaacagcga caccaataaa aacaccaatg ccgtctgaac ggcaaatttg
     6661 atgcccgaaa gcagtttctt tttcatacgc tctctcaaac ggtacgcccg cgcaaccggc
     6721 aggcaaacaa aaagccaagt ctcaaaactt ggcttccggt tatctggtgg gtcgtgagcg
     6781 attcgaacgc tcgaccaacg gattaaaagt ccgctgctct accggctgag ctaacgaccc
     6841 gataagccgt gcattataca gcaccatcct acctcgtcaa gcaaatttta caggcttaat
     6901 tgcagaccac tgtttgcacg ggatattttg acaacggatt ttcacaatcc gccgcatacc
     6961 gtgtaaaagt tcgcacaagg aaaagcaaac cgcccgaaat caatgtacac tttccgcccg
     7021 tttcccaaac tgcacacaga aacacacatt atgaacatac aaaacatccg caccctcctc
     7081 gacaccgtcg ccgttccgaa tacggcacgc acgctcggcg gcgaaaaggc cgtccgttcg
     7141 gtcgaacagc gttcagacgg catccatatc gccctgcatt tcggcttccc tgtcgcgcac
     7201 atcgccgccg ccatcgccga ttccgtacaa gaaaccctga tgcccgaaac aggcgacaca
     7261 cacatccatc tgtccatgga cacggaaatc ggcacacata aagtccaacc tggcgttacc
     7321 accatcaaag gtgtgaaaaa catcattgcc gtcgcctccg gcaaaggcgg cgtgggcaaa
     7381 tcaacaacca ccgccaacct tgccgccgca atggcgcgca tgggcgcgcg cgtcggcgta
     7441 ctcgatgccg acctttacgg cccgagccaa ccgaccatgt tgggtgtgga cgaccgcaaa
     7501 cccgatcaga aaaaccaaaa actcattccc gtcgaatctt cagacggcat acaggtcatg
     7561 tctatcggct ttctcgtcga taccgaccaa gccgtcgtct ggcgcgggcc gatggtcagc
     7621 caagccttgc agcagctgat gttccaaagc gagtgggacg aagtggacta tcttttcatc
     7681 gacctgcccc ccggtaccgg cgacatccag ctcacgctgt cccagcgcat ccccgtaacc
     7741 ggttccgtca tcgtaaccac gccgcaggac atcgccctga tagacgcgcg caaagccgtg
     7801 gatatgttcc gcaaagtcaa cattcccatt ttgggcgtat tggaaaatat gtccgtccac
     7861 atctgctcca actgcggaca cagcgaagca ctgttcggca cggacggcgg caaagatttg
     7921 gcggcacgcc tcaacgtccc cctgctcgga cagcttcccc taagcctgcc cgtgcgcgaa
     7981 gccatggacg gcggcacacc ggcgcaactg ttcgacgaac accccgccat cgccaaaatc
     8041 tacaccgatg ccgcattcca aatcgccctg agcattgccg acaaaggcaa agacttcagc
     8101 agccgcttcc ctaaaattgt cgtcgaataa agccgcgtcc gaaaccgcaa cagcaatgcc
     8161 gtcccaagcc ccgcgcctgc cgatatgccg tcattcccgc gcaggcggga atctagaacg
     8221 tggaatctaa gaaaccgtgt tgtaaaggca gaccgatgcc gtcattccca cgaaagtggg
     8281 aatctagaac gtaaaatctc aagaaaccgt tatacccgat aagtttctgc accgacaggt
     8341 ctggattccc gcctgcgcgg gaatgacggt tcgggtattt ctgacgattt aggcattccc
     8401 gacaaggtgg attttcaagg tgttgtatag ggtgtaggag gattcgtaaa aggtgagtta
     8461 tagggtgggc ttcagcccac cgattccaac gattccacca atcctacacc gttcccatag
     8521 actcaaatca acacagaaac ttatgcgccg tcattcccgc gcaggcggga atctagacct
     8581 tagaacaaca gcaatattca aagattataa aagacccgtc attcccgcgc aggcgggaat
     8641 ctagacctta gaacaacagt aatattcaaa ggttatctga aagtccggga t
//
LOCUS       DAWKOD010000076         8588 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000076 DAWKOD010000000
VERSION     DAWKOD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.076"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(240..3092)
                     /gene="gcvP"
                     /locus_tag="WJK73_001297"
     CDS             complement(240..3092)
                     /gene="gcvP"
                     /locus_tag="WJK73_001297"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ7557774.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSTSAEQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3123..3482)
                     /locus_tag="WJK73_001298"
     CDS             complement(3123..3482)
                     /locus_tag="WJK73_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557775.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3430..3660
                     /locus_tag="WJK73_001299"
     CDS             3430..3660
                     /locus_tag="WJK73_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7557776.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3653..4294)
                     /locus_tag="WJK73_001300"
     CDS             complement(3653..4294)
                     /locus_tag="WJK73_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ7557777.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4284..4835)
                     /locus_tag="WJK73_001301"
     CDS             complement(4284..4835)
                     /locus_tag="WJK73_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ7557778.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4839..5357)
                     /locus_tag="WJK73_001302"
     CDS             complement(4839..5357)
                     /locus_tag="WJK73_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ7557779.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVK
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNHLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5357..7018)
                     /gene="pqiB"
                     /locus_tag="WJK73_001303"
     CDS             complement(5357..7018)
                     /gene="pqiB"
                     /locus_tag="WJK73_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ7557780.1"
                     /translation="MTDNSPPPNRHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7011..8471)
                     /locus_tag="WJK73_001304"
     CDS             complement(7011..8471)
                     /locus_tag="WJK73_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ7557781.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1908 a         2210 c         2437 g         2033 t
ORIGIN      
        1 gtttgtccgt gtggaaatga catcaaaggg tgtgaatagg cttttcatct ttgaaatctg
       61 tctgcccgat cggatgaata aaaagtagga tgataaaaaa actgatgtat ggatactggt
      121 ttaacccatt tgttttaaag ttatagtcga gatacgtgta tcggtgcaac cagtctgccg
      181 tcgacgaaaa tgccgtctga aagtgtttca gacggcattt gtcttcgggg tttcaacagt
      241 cagtcttcat aattttccat cggtgggcag ctgcacacga gattgcggtc gccgtacacg
      301 tcgtccacgc ggttgacgaa gggccaaaac ttgtgttcgc ggacgaacgg caacgggaag
      361 acggcttctt cgcgggaata cggatgcgcc cagttgccgg ttatatctgc ggcggtgtgc
      421 ggagcattta ccagcggatt atcgtctttc ggccattcgc cgtcgatgac tttctgcact
      481 tcgcggcgaa tggatttcag ggcggcgatg aagcggtcga gttcggcttt gctttcgctc
      541 tcggtcggct cgatcatcag cgtaccggca acagggaagg aaaccgtcgg cgcgtggaag
      601 ccgtagtcca tcaggcgttt ggcgatgtcg gtttcggtaa tgccactttc ggctttgagc
      661 ggacgcaaat caacgataca ttcgtgcgcg acgcggccgt ttttgcctgt gtacagaatc
      721 ggatagtctt cgcccagccg tttggcgacg tagttggcgt taagcaatgc ccaacgtgtt
      781 gcctgctcca tgccttgttt gcccatcatg gtcaggtaca tccaagtaat cggcaggatg
      841 gatgcagaac catatgctgc ggcagccacg gcggtttgct cggcacttgt gctgtgggtg
      901 tcggtcaaag cgtgtcctgg tgcgaacggg gcgaggtggg ctttcaagcc aatcggaccc
      961 atgcccgggc cgccgccgcc gtgagggata cagaaggttt tgtgcaggtt catgtgcaac
     1021 acgtccgcgc cgacttcggc aggctgcatg ataccgattt gagcgttgag gttggcgccg
     1081 tccatgtaaa cctgtccgcc gttttcatgg atgatgcggc agatgtcgcg gatgccttct
     1141 tcgtatacgc cgtgggtgga cgggtaggta atcatgatgg cggacaaggc gtcgcggtgt
     1201 tgttcggctt tggctttcaa atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg
     1261 acgacgactt tcaaaccgag catggcggcg gtggcggggt tggtgccgtg ggcggatttg
     1321 ggaatcaggc agatgttgcg gtgtccctcg ccgttggctt cctgatagcg gcggatggag
     1381 agcataccgc tgtattcgcc ctgcgcgccg gagttgggct ggaaggaaat cgcgtcaaag
     1441 ccggtgatgg ctttcaggct gttttccatg tcggcaagca attcgcggta gccggcggtt
     1501 tgcgcttcgg gagcgtaagg gtggatgtcg gagaactccg cccaagtaat cggcagcatt
     1561 tccgcagtcg cattgagttt catggtgcag ctgcccaatg aaatcatact gcggttcatc
     1621 gccaagtcgc ggtcttcgag ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa
     1681 ctgttgaaca caggatgttg cagaatgtcg tcttgacgca gcagttcggc gttcagacgg
     1741 cctttgacat catcggcaaa tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca
     1801 accaaatctt cgcatgccga tgtttcgtgg aatgcagccg caacttgagt atcgttaacg
     1861 cggcgcaggt tatagcccga ttccaaagca gcggcaaaca cttggtctgc tttctctttg
     1921 ttgccgaaat cgacggtaac ggtatcgaag aagaccttat gaaccacatt caggccgtct
     1981 gaaaccagcg catcggcaaa ggcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc
     2041 acaccttcag ggccgtggta aacggcgtac atacccgcca agttcgccag caatgcctgc
     2101 gcggtacaaa tattggatgt cgctttttcg cggcggatgt gttgctcgcg ggtggacaac
     2161 gccatgcgca aggcaggttt gcccgatgcg tctttggata cgccgatgat acggcccggg
     2221 gcggaacgtt tgaactcgtc tttaaacgcg aaataagcag catgcggacc gccgaagccc
     2281 atcggtacgc cgaagcgttg ggtgttgccc aaagcaatat ccgcgcccaa ttcagccgga
     2341 gacttcagca aaaccaagct cataatgtcg gcggcaacgg ccacgattgt gcctttggct
     2401 ttcagacggc cgataacgtc ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg
     2461 aacagcgcgc cgaagtattc gccttcatcc gctttggcaa aatcgccgac caccagctcg
     2521 aagccgaaat acttggcacg ggttttcatc acgtccaaag tttgcggata cacgcgctcg
     2581 tccacaaaga aacgctcgga ttttaccttg cccacgcggt gcgccatcgc catcgcttcg
     2641 gcggcggcgg tcgcttcgtc cagcaaagac gcgcccgcca cagggaaacc ggtcaaatcg
     2701 atacacactt gttggaagtt caacaaagct tccaaacgac cctgggcaat ttccgcctga
     2761 tacggcgtgt aggcggtata ccagcccgga ttttccaata cgttgcgcaa aatcacattc
     2821 ggcacgcggg tcgggtaata gcctaaaccg atataggact tgttgatcac gtttttcgac
     2881 gcaatgcctt tcaattttgc caacgcgtcc gcttcggtca gggcatcggg caaatcgagt
     2941 tcagacggca tacggatgct ttgcggcacg gtgttgccga caaaatcgtc catacttttc
     3001 tcgccgacag cggcaagcaa cgccgcttcg tcgccgaaac tcaaatgccg cgcggcaaat
     3061 tcgtcggggt tgaacagttc ggacagtttc atttctactc cttcgggttt gtagggctgc
     3121 catcaggcag aatttttaga catcggatga ttcgggcaag ggtagcacaa aagacgggat
     3181 aatggcggac aatgcgccag aatgcagcag gtcaggacaa gcagcgccac aagcggcagc
     3241 gataagggaa agccgccgcc caaaaccatg aaacctgcag gcaccagcca agccaatgga
     3301 tacgccggaa gcggaaaaac aatattgagc agaaccaaaa tcacgacaaa cagcggcaga
     3361 cccgccatca ggttggcacg cctgtatgtt gcgtacgggt cggatgcttc ttcaggttcg
     3421 caatattcca tgatttcagc ctgtccgggc tttatccaca gcaggctcgg acggtcggtc
     3481 atgccgtctg aaccggcagg ccgtcttttt caaaaaacgc cataccgctg caacttcatg
     3541 cggctgtatg gcgtaggctt tgcacttcag acggcatatg cgtcccgaag cagtttggtg
     3601 tcccagtcgg ttcatgatta tataaacaac ttgggtgcgg ataaaccttt gtttatctaa
     3661 acccctgttt tctcaaaaat tgattcaaat cttcggcaaa tttccgatag cctttgccgt
     3721 tggcgtggat ttggtcggat ttcagattat tatcgcccaa aatttccgcc cacgcgccgc
     3781 cgaacagcgg aatgccgtat tcctcggaca aatcctcata cagcggatgg tcgctcaaat
     3841 gtccgaacaa cgcgcccagt gtgatgtgcg gcacgccgac gaggacggcg gggatgtttt
     3901 ccttctgcac ggtttcgatg attttcgcga tattggcgcg ggtctgctcc tcgggaactt
     3961 tgcgtagaaa gtcgttgccg cctatgccga caatcacaag cttgggtttg cgtgccaaca
     4021 gcgcgggcag gcgcgacagg gcttgggcgg atgtatcgcc cgatacgccg ccgttgacaa
     4081 tattccaacc cgtcagtttt tgcagttgcg cggggtagga ttcgccgggg tttgcgccgt
     4141 agccgaaggt aagcgaatcg cccaaggcaa gtacggtgct tccttcggga attttggcgt
     4201 gggttcgggc ggattttctg ccgcaggcgg taaggagcaa cgcgcctgcg ccgaggagga
     4261 aggttcttct gttcattggt ttttcagatg gcatcgagcc atttcgggtg gtatttcagg
     4321 gtttcacggt aaaccggaca gctttcggcg tgcgcgcctt tgaggtagcc ggtactcatc
     4381 agaaattcgc cgacgatttc gccgccgacg aatttgaaat gttttttaaa gagtttaacc
     4441 cattcgtctt tgcttcgcgg atggtgcgcg tcgagccagt tcttgaacga gccgtattct
     4501 tgttgcaacg cttggatttg ccgtgcattg aaaatggcgg catcgatttt caggcggttg
     4561 cggacgatgc ccgcgtcggc aagcaggcgt tcgcggtcgg tgtcgtcgaa ggcggcaacg
     4621 gtattgatgt cgaacccttc aaatgctgtc tgaaacgcct gccgcttctt cagcatcagc
     4681 gtccagttta atcctgcctg attgatttcc aacaccagcc gctcaaacaa ttcattgtcg
     4741 tcctcaatcg gaaaaccgta ttgcgtgtcg tggtaatgtt tgttcgggtt atcggtgttt
     4801 tcgggaagtg aggcagcaaa ttcgcaatag ttcacggttt actcgaccat ctgttgcgcc
     4861 gcctgtttca gtccctgttc gagtgcggcg gtcatagcgg cgtagccgtc accctgctgt
     4921 tcggtttcga tatggaaggg tctgttcgta ccgtcgggta ggacggcgta gccgctgatg
     4981 agggttttgc ccgtgtagct gccttggaat gcgtcgatat agaccgtcca tttttcggta
     5041 ctgccgctgc gtgaggcagg aacaaagatg cgtgtgctgt ccaaatggtt gaatgcattg
     5101 ctcaacgccg cttcgagcat atcgtccaag gtgtctgccc aaacgtggtt ttgtgcggtg
     5161 ttgaggcggt aggggtcggt ttgatagacc agtccgccgc gtttgagcgg ttcggcaaga
     5221 cggactttga cggcggtttc gccgccttgc gttgcaggac ggatgtagcg gctgtcgggc
     5281 aacacgaaat attgtgtgct ttgcacagta ccgcaggcgg caagcgtcag ggcggcggca
     5341 atcgggaaga ggcgcattat cggcttcctt tcgggatggg gtctttgctg ctgctgttga
     5401 aaatcagcgc gttgggtttt tctttcaaag tattaattac gggttgaacg tcttttaagg
     5461 ttttgtccaa actttgcaac gtattttgta cgtcgccgta gataggcgat tgaggcgata
     5521 cgccttgcag ggttgtgcgc aactctttca gggtttggtt cagttcgttc ggaatgtttt
     5581 gtgtctgcgg tttgccgacc agtttgtcga tggagcttag ggcggcattg gcagatttga
     5641 gtgtggattt gagctcggca agcgaaccgt tcaattcggc aaccgtctta tctaaaggca
     5701 gtttgtcgaa cttgtccagc aaatccacca ctttcgcctg caaatcgtcc aaaccgccgc
     5761 cttgtgtagc gatgacggta tcgcctgcat aaacggtatg cggtcgcagc ttgggcgaag
     5821 acgaaggctg atcgttcaac tcaatcattt tgctgccggt cagcaggttg ttgctggaaa
     5881 tggtggcggt caggcctttg tttaaggccg tctgaaattg ttgtttccaa tgttctttgc
     5941 tttgctcgtc ggcattgatt tccaaacggg aaggctcgat gcggatgcgt acgggaatcc
     6001 aaccgttttc aaacagatgc aggctgtcgt tgcggtcgaa ataagggaca tcggaaacca
     6061 cgccgacatt cagccctttg tactcgacgg gcgaaccgac ggtcagtccg cgcacggatt
     6121 gtttgaaaaa cgcggtgtaa tacagcgagc ggtcgtcagg caggttggcg acttcgctgc
     6181 ggctgtcgta aagcgtgaag ctgtcttcgc ttttgacgtt tttactgttt ttggttttcg
     6241 gcgaatcaaa tgaaatcgcg cccgacagca gggcaggcag aggggcggaa ttgagtttga
     6301 tgccgctgcc tgtggtttcg atattaatgc cgctttccag ccaaaaacgg ctggcggaat
     6361 gaatcagttt gtcgttgggg ctttggatga agatggtgta atgcacgctt tggtcggacg
     6421 ggtcgaaatg cgcgctttcg atttgcccga ccataaagtt ttcatacaaa acagggctgt
     6481 tgacgttgag gatgcggtcg tttttcccaa tcaaattcaa gcgcagtccg ctttgcccga
     6541 tggcggtaac gggcggaatg tcctgcactt ggaacacgtc ttttgcctcg tcgcttttgc
     6601 cgggtgtaaa agcgatgtac gaacccgaaa gcagcgtacc caaaccggtt acgccgcttt
     6661 ggtcgatacg cggcttgacc acccaaaact gtgtgtcgct gcggatgagg ccggatacgt
     6721 ccgcattgag ttgggcggtt acttccacac ctttctggtc gtcgcgcagt ttgattcggg
     6781 taacgcgtcc gacatcgatg ctcaatacct taatgaccgt attgttgacc tcaatgcctt
     6841 ccgcgctgtc catcaagagc gtaaccacag gccccctgtt gcggatttcc ttaatccaca
     6901 gccagccacc ggcaatcagc gcaatcagcg ggaccagcca gacggcggag agcaaggtgt
     6961 tgtttttgcg gacgcgtgct tgggcgtgtc tgtttggagg agggctgttg tcagtcatgt
     7021 ttttccgttt cattgaaagc aatgccgtct gaagcgcgtt tgtcccaaag caggcgcggg
     7081 tcgaaataat aggcggacag catcgtcaga atcacgacca ggcagaaata gactgccgca
     7141 ctgcccggaa tgacgcgcgc ggcataagtg tggaacgaac acatcaaaat aataatcaca
     7201 aaaatatcaa tcatcgacca gcggccgacc gcttcggtga tgcggtagag gtgcgacaat
     7261 ttctttgcac ccgttggcaa agcgaagcgg gcggacgcaa tcaaaaccga cattgccgca
     7321 atcttcagta ccggcaccaa aatactcgcg ctgaaaataa ccgccgcaat cagcctgtcg
     7381 ccctcgtccc acatataagc gatgccgtta aggatggtat tgacctccgt ggcggcagga
     7441 ttggacgaaa tcataatcgg caggatattg gcagggaaat acaaaataac cgccgccgtc
     7501 agaaacgccg acgaaatact cagacttttc ggccgtcggc ggtacagttc cgcaccgcac
     7561 acgccgcagg gggattcggc accgtctcgg aaatacaggc agcggctgca acaggttttg
     7621 ccttctgatg ccgtctgaac cgcattatcc cccgtcagcc gcccgatttg aaaatacacc
     7681 caatgctggg gaaccgatac cgaagtccga atcagcataa ccgacagcgc gaacatcaga
     7741 taaaacgccg acccgaagcg aacctctgcc acagacgaga gcttgatata cgccaccagc
     7801 gtggaaacaa aaaacacatc caccatcatc gcctgcctca agcgcaccat cacacgcgtt
     7861 gccaaacgca gcgcaggata cacccgtttc cgtatcagcg cggcatagac atacaggcac
     7921 agcagcagaa acagaaccgg cgcgccgaag gtcagcacaa acatcacttc ggccaaaaaa
     7981 ccataatcct gaaacaccat caggcgcatc atctcgggca gcgaaaggac ggatgccgca
     8041 cccggtatcc cgacctcgat atacgtcata ccgtaagcaa acgccatcaa aatcagcgaa
     8101 gccgccgcat aagcgggcgg ggcggaaaaa ggatgcctgc ccaccctgaa gagcttatag
     8161 ccgcagcgcg ggcagaacgc cgcctccccc ctgtccaacc gaggcacatc cgcgcggcag
     8221 ccgcattccg ggcaatctac cgtatgcggc ggtagggctt cgtcgcggca gttgccgcga
     8281 aggctgtggg ggatattttc ggcaaacggc ttcatcggga aagtggcaaa tataagtggc
     8341 ggcattataa aggaaaaaag gcgatacacc caatcaggat gcaaaaccgg caggttcggc
     8401 agtatgaaca aacccttgtc gtccggcaaa aatatttttt cgagcggctg tccgccgact
     8461 gccgaaccca tttgataaaa tcccgattct gtaaagaata gtaaaatacc tgcttaaaaa
     8521 catcacgcaa atgatatttt ttccaaaaat atgtgataat aaatttcgtt tatgattctt
     8581 tattattt
//
LOCUS       DAWKOD010000077         8564 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000077 DAWKOD010000000
VERSION     DAWKOD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.077"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(77..595)
                     /locus_tag="WJK73_001305"
     CDS             complement(77..595)
                     /locus_tag="WJK73_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ7557782.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(615..2000)
                     /locus_tag="WJK73_001306"
     CDS             complement(615..2000)
                     /locus_tag="WJK73_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ7557783.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2239..3780
                     /locus_tag="WJK73_001307"
     CDS             2239..3780
                     /locus_tag="WJK73_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ7557784.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITLPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3809..4408
                     /locus_tag="WJK73_001308"
     CDS             3809..4408
                     /locus_tag="WJK73_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ7557785.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4405..5010
                     /gene="nadD"
                     /locus_tag="WJK73_001309"
     CDS             4405..5010
                     /gene="nadD"
                     /locus_tag="WJK73_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ7557786.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLYQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5069..5455
                     /gene="rsfS"
                     /locus_tag="WJK73_001310"
     CDS             5069..5455
                     /gene="rsfS"
                     /locus_tag="WJK73_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ7557787.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5509..5979
                     /gene="rlmH"
                     /locus_tag="WJK73_001311"
     CDS             5509..5979
                     /gene="rlmH"
                     /locus_tag="WJK73_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ7557788.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6054..6320
                     /locus_tag="WJK73_001312"
     CDS             6054..6320
                     /locus_tag="WJK73_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ7557789.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6477..7160
                     /locus_tag="WJK73_001313"
     CDS             6477..7160
                     /locus_tag="WJK73_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ7557790.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7284..7796
                     /gene="coaD"
                     /locus_tag="WJK73_001314"
     CDS             7284..7796
                     /gene="coaD"
                     /locus_tag="WJK73_001314"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ7557791.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7828..8481
                     /locus_tag="WJK73_001315"
     CDS             7828..8481
                     /locus_tag="WJK73_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ7557792.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1862 a         2270 c         2508 g         1924 t
ORIGIN      
        1 aatgtaatcc attctgttcc cgatgtttat gccgtctgaa acccatcctg tgtcgggctt
       61 cagacggcat attgcttcaa agcaggtttt ccgaggcaac ccagttcaga atatcggctt
      121 cgaccgcttc gggcgtgccg gggttggcga ttttgacccc gtattcgcct tcgcccagtt
      181 cctcgaggat ttccagttgc gaatcgagca tccctgcttt catgtaatgt cctttgcgcg
      241 acatcatgcg ctcgaggttg atgtcttgcg gcggactgag gtggatgaag gcagctttgc
      301 cttcggctcc gcgcagaatg tcgcggtagc cgcgtttgag ggcggaacag gttacgatgg
      361 tgtggttcgc accgttttgc gcctgttgcg tcatccagtc gcgcagattg cccaaccacg
      421 gatagcggtc ttcatcggtc agcggaatac ccgcgcccat cttgtcgcgg ttggcttggg
      481 tgtggaactc gtcgccttcg gcatagggac attgaccgag gtgtttctgc agggacagcg
      541 cggcggtggt cttgccgcag ccgcatacgc ccataacgac aaaatgcgta gtcatttact
      601 atcctttccg tctgtcagac gatggcgaac agcagtgcgg acaaggcaaa gccgatgagt
      661 gcgatgaggg tttggttgac cgtccaggtt ttcagcgtgg tcggtacgtc catgtccaag
      721 agacggccga ccagccagaa gccggagtcg ttgaagtggc tgcaaccgac cgaacctgcc
      781 gccgttgcca atacgataca ggcgagctgc cagtcggtaa agccggcggc ggcaacggca
      841 ggagccatca gcgcggcggc ggtggtcagg gcgacggttg ccgaaccttg cgcgatacgc
      901 agtgccaagg cgacaaggaa acagcccaaa aggacgggaa tgcccaaatc cgccatgctg
      961 tcggcgagtg ccttgccgat gccggaagcg cgcaaaacgc cgccgaacat accgcccgcg
     1021 ccggtaatca gaatcacgga acagacgggg gcgagtgcgc cgtccacggt tttttccaac
     1081 gcgctgccgc tttcgccgcg tttgcgtccc aagacaaaca gtgcgaccaa tacggaaatc
     1141 agaagggcga tcggtgtcga accgattatt tttgccgtct gaacccaggt ttcgtccgca
     1201 cttacgagtt tttcgctgat gagggccgat acgccggtat tcaggaaaat cagcagcatg
     1261 ggaatcagca tgatggcgac gaccgttcct gctttggcag gttctttcgg caggtcgttg
     1321 tcttgcgtgc cgccgctgag cagttcggga acgggaacat ggatggtgcg ccccaacact
     1381 ttgccgagca tatagccgct gaaataccat gtgatgaagg cggtcggcag acccaaaatc
     1441 aaaacttggc cgatgttcgc gccgtaaaat tcggaagcgg caatcgggcc cggatggggc
     1501 ggcaggaaga cgtgcatgac ggaaaatgcg ccgatggagg caagcgcgaa gggcagtacg
     1561 tcctgtttca tgcgccgtgc ggtggcgaac acgatgggca gcatgacgat tagtccggca
     1621 tcgaagaaaa tcgggaagcc gaaaatcagc gaggcaacgc ccagcgcgaa cggtgcgcgt
     1681 ttttcgccga acatccggat cagcgcgtcc gccagcgact gtgcgccgcc ggatgtttcg
     1741 accagacgtc cgagcatcgc gcccaggccg accagaagcg ccacgccgcc gagcgtgccg
     1801 ccgaagtttt tgaccagtac gtcgttgaca atgctgcctg tgggcaaacc ggttgccaaa
     1861 gccgtcagca ggctgacgat gaccagtgtc agcagcgcgt ggatgcggaa tttgacgatt
     1921 aaaatcagaa tgaggatgat tgccgccgcc gaaatgccca acagggtttg cgcggacagc
     1981 gtctgtgtcc agccgtccat ttgtaaagcc tttctctgta ataaaagtac aaaattgtca
     2041 ggttttttta agttgtgcga aaacgatagc acaaatcggg cggtaagttg tttgtctgaa
     2101 gttatattcc tgtttatttg aacgatttat ttcagacggc gggaggaagg cggatacggt
     2161 atgtagtcgg attgaaatat acggtatgtg gggcttttgg gtacaatcgc aacatcagtg
     2221 tttcaagtgg ggaaggcgat ggatggacgg cgttgggcgg tatggggtgc ttttgccctg
     2281 ctgccttcgg cttttttggc ggtaatggtc gttgcgcctt tgtgggcggt ggcggcgtat
     2341 gacggtttgg cgtggcgcgc ggtgctgtcg gatgcctata tgctcaaacg tttggcgtgg
     2401 acggtatttc aggcagcggc aacctgtgtg ctggtgctgc ctttgggcgt gcctgtcgcg
     2461 tgggtgctgg cgcggctggc gtttccgggg cgggctttgg tgctgcgcct gctgatgctg
     2521 ccttttgtga tgcccacgtt ggtggcgggc gtgggcgtgc tggctctgtt cggggcggac
     2581 ggcctgttgt ggcgcggacg gcaggatacg ccgtatctgt tgttgtacgg caatgtgttt
     2641 ttcaaccttc ctgtgttggt cagggcggcg tatcaggggt ttgtgcaagt gcctgcggca
     2701 cggcttcaga cggcacggac gttgggcgcg ggggcgtggc ggcggttttg ggacattgaa
     2761 atgcccgttt tgcgcccgtg gcttgccggc ggcgtgtgcc ttgtcttcct gtattgtttt
     2821 tcggggttcg ggctggcatt gctgctgggc ggcagccgtt atgccacggt cgaagtggaa
     2881 atttaccagt tggtcatgtt cgaactcgat atggcggggg cttcggcact ggtgtggctg
     2941 gtgttggggg taacggcggc ggcagggttg ctgtatgcgt ggttcggcag gcgcgcggtt
     3001 tcggataagg cggtttcacc cgtgatgccg tcgccgccgc agtcggtcgg gggatatgtg
     3061 ctgctggcgt ttgcggcggc ggtgttgtct gtgtgctgcc tgtttccttt gttggcaatt
     3121 gttgtgaaag cgtggtcggc cggcgaatcg tggcgtgtgt taatggaaag tgaaacgagg
     3181 caggcagtgt ggaatacttt gcgcttctcg gcggcggcgg tgtttgcggc ggcggttttg
     3241 ggtgtggtgt atgcggcggc ggcgcggcgg tcagcgtgga tgcgcgggct gatgtttttg
     3301 ccgtttatgg tgtcgccggt ttgtgtttcg gcgggcgtgc tgctgcttta tccggggtgg
     3361 acggcttcgt tgcccttgct gctggcgatg tatgcgctgc tggcgtatcc gtttgtggca
     3421 aaagatgttt tatcagcctg ggatgcactg ccgccggatt acggcagggc ggcggcgggt
     3481 ttgggtgcaa acggctttca gacggcatgc cgtattacac tccccctctt gaaaccggcg
     3541 ttgcggcgcg gtctgacttt ggcggcggcg acgtgtgtgg gcgaatttgc ggcgacattg
     3601 ttcctgtcgc gtccggaatg gcagacgttg acgactttga tttatgccta tttggggcgt
     3661 gcgggtgagg ataattatgc gcgggcaatg gtgttgacat tgctgttgtc ggcatttgcg
     3721 gtgtgcattt tcctgctgtt ggacggcggc gaaggcggaa aacggacgga aacgttataa
     3781 tgcgaaccct ttttcagagg acggcaaaat gagcgagttg gataacatcc ttgcccacaa
     3841 ccggcagttt gtcgaatcgg gcgaatatga aaaatacttt accgacaaat accccgaacg
     3901 cgggctggcg gttttgtcct gtatggatgc gcggattatc gggctgctgc ccgacgcgtt
     3961 gggtttgaaa aacggcgatg ccaagctgat taaaaatgcc ggcgcgctgg ttacgcaccc
     4021 gtggggttcg gtgatgcgga gccttttggt tgccgtgttt gaattgaagg tcagagagat
     4081 tatggtcatc gcccatcacg attgcggtat gcaggggctg aatgccgaag aattcctcgg
     4141 gcgcgtccgg gaaagccgga ttcccgaaga ccgtatcgaa accctgcgtt atgccggtat
     4201 cgacctcgac ggctggctga ccggtttcga caacgtcgaa gacagcgtgc gccacacggt
     4261 ggaccttatc cgtaaccatc cgctgatgcc gcgccatatt gccgttcacg gattggtcat
     4321 ccatcccgtt accggcaaac tgacgctggt tgtggacggc agtgtttcag acggcatgga
     4381 cttatcggaa ggaatggaaa catcatgaag aaaatcggac tgttcggcgg cactttcgac
     4441 ccgatacaca acgggcatct ccacatcgcc cgcgcctttg ccgacgaaat cggcttggac
     4501 gcggttgttt tcctgccgac aggcggcccg tatcacaaag acgcagcctc cgcttccgcc
     4561 gccgaccgcc ttgccatggt cgaactggcg acggcagaag acgcgcgttt tgccgtcagc
     4621 gattgcgaca tcgtccgaga aggtgcaacc tatacttttg ataccgtcca aatcttccgc
     4681 cagcagttcc catccgcgca actctggtgg ctgatgggca gcgacagcct gatgaagctg
     4741 cacacatgga aaaaatggca gatgctcgtg cgcgaaacca atatcgccgt cgccatgcgg
     4801 cagggcgaca gtctgtacca aaccccgcgc gaactgcacg cgtggctggg caagtccctt
     4861 caggacggca gcgtccgcat cttgtccgcc ccgatgcata atgtgtcgtc aacggaaatc
     4921 cgccgcaacc ttgccggcca aggcgtttca gacggcatcc cgcctgccgc cgcacgctac
     4981 atccgcgaac acggtttgta tgaaaaataa agtcaaatca gtaagaaacg gctataatgc
     5041 cgtctgaaaa catcccgtta ggaacataat gaacgaacaa gaactgcaag acctgcaaaa
     5101 aatggtcggg gtcgccgtca acgccctcga agacatcaaa gccaaagaca tttccgttct
     5161 cgaaacgcaa gacaaaactt cgctgtttgc cagaatgatt atcgccagcg gcgacagtac
     5221 gcgccaagtc aaagcactgg caaacaacgt tgccgtcgat ttgaaagaag ccggttttga
     5281 aatcctcagc accgaaggcg acagcggcga atggacgctc gttgatgcag gagacctcgt
     5341 cgtccacgtt atgctccccg ccgtgcgcga cttctatgat atcgacaccc tttggggcgg
     5401 cgagaaaccg agttttcacg ccggaatgca gaagccgtgg cacgcggcag actgattccc
     5461 gatgccgtct gaatgttcag acggcatttt cctgtaaggg agaaagcatt gaacatcacc
     5521 gttttggcag tcggcaccaa aatgccgcgc tgggttgatg aggccgtcgc cgaatacgcc
     5581 aaacgcttcg gacgcgacgt cgcctacgca ctcaaagaaa tcaaacccga aaaacgcggc
     5641 gcgggcgtga atgccgccca aggtatggcg gcggaagaaa aacgcatcct tgaagccatt
     5701 ccgcaaggcg cgttcctcgt cgttcttgac gaacgcggca aagcaccgac ctccgtcgag
     5761 ctggcggaac acctcaaaag ctggcagcaa aacggcgaac acgtctgctt cgtcatcggc
     5821 ggcgcggacg gtatgaccga ccgcctcaaa cagcaggccc gcatgatgat gcgcctgtcc
     5881 agcctgaccc tgccgcacgg gatggtgcgc gtccttctga ccgaacaact ctaccgcgcc
     5941 gcctccatcc tgcacaacca cccctaccat cgggaataag aaggcttttg tccgcactcc
     6001 caatcggtta aaatcccccc gttatttctg aacacacagc aaaggaatcc gctatggcac
     6061 gcatggtatt ctgcgtcaag ctcaacaaag aagccgaagg catgaaattt ccgccgcttc
     6121 ccaacgaatt gggcaagcgc atttttgaaa acgtatcgca agaagcgtgg gcggcgtgga
     6181 cgcgccacca aacgatgctg attaacgaaa accgtttaag cctcgccgat ccgcgcgcgc
     6241 gcgaatacct ggctcagcag atggagcagt atttcttcgg cgacggcgcg gatgccgttc
     6301 agggatacgt tccgcaataa cggttttccg ttttgaacac aggctgtccg aaactgcttc
     6361 agacggcctt taaaatacgc cggacaactt tatcttcacg caaccggaca aattttgaca
     6421 ttgggcaatg ttaaaatccc ccattattcc caaccgcgct ttcaggagca gatgatatgc
     6481 aacacgacgt ttacgactac accgcgcata cggtttctaa aaacaccgtc ctgcagaaaa
     6541 cctaccgcct gctcggattt tcattcattc cggctgccgc aggcgcggca cttgccgcca
     6601 atgccggttt caatttttac gccgccttcg gttcgcgctg gatagggttt gccgtcgtgt
     6661 tggcgttttt ctacggtatg atccacttca tcgagaaaaa ccgttacagc aataccggcg
     6721 ttaccctgct gatggtattc acattcggta tgggcgtatt gatcggcccc gtgctgcaat
     6781 acgcgctcca tattgccgac ggtgcgaaaa tcgtcggcat tgccgccgcg atgaccgccg
     6841 ccgtcttttt aacgatgtcc gccttggcgc gccgaacccg gctcgatatg aacgcgctcg
     6901 gacgcttcct gaccgtaggt gcggtcattc tgatggtcgc cgtggttgcc aatctgtttt
     6961 tgggtattcc cgcactcgcc ctgaccattt ccgccggttt tgtcttgttc agttccttaa
     7021 tgattatgtg gcaggtacgc accgtcatcg acggcggcga agacagccat atcagcgcgg
     7081 cactgacact gtttatctcg ctttacaaca tcttcagcag cctgctgaac atcctgttgt
     7141 ctttaaacgg cgaggattga tgtcggtaaa acgccgtccg actatgccgt ctgaaaatgc
     7201 ttcagacggc attttacttt gggcatacaa tacggatgca cacgcaccaa cacacagccg
     7261 gctatgaagc cgggaggaat caaatgttac cgaatacacc gcgccgcgcc gtttatgccg
     7321 gcagtttcga tccgcccaca ttggggcatc tgtggatgat acggcaggcg caatctatgt
     7381 ttgacgaact catcgtcgcc atcggcatta accccgacaa acgcagcacc tataccgtcg
     7441 ctgaaaggca ggatatgttg tgcgatatta ctaaaatgtt tcccaacgtc agaaccgatg
     7501 tatttgaaaa ccgatttctg gtgcattatg cccgtgaggt agatgcagga ttcatcgtgc
     7561 gcggcatccg ttctgcttcg gattacgaat acgaacgttc catgcgccac atcaacagcg
     7621 acctcgcccc cgaaatatcc accgtattcc tcatgccgcc gcgcgaaatc gccgaagtgt
     7681 cgtccactat ggtcaaagga ctggtcgggc cggaaggctg gacggaaacg atccaccgct
     7741 acgtgccgca agctgtgtac gaaaaaatcc ttgccgaaca tcaacacgaa aattgaaccc
     7801 ttctattttt gcaaggcata tatggatatg ttggaaattt tgttccgcca tcaagacttc
     7861 gtcgccatca acaaaccggg cggcatatcc gtccaccaag acagcggcga gaccggactg
     7921 gcccgaacac tcgccataca gttgggcgta gagcgtgtgt ggctgctgca ccgattggac
     7981 aaacagacca gcggcatttt actgtttgcc ctcaacagag aaagcgcgtc cgcccttagc
     8041 ggacagtttg ccggcaaaag catcaaaaaa acctatttgg cactatccga ccgcaaaccg
     8101 tccaaaaagc agggttggat taaaggcgga atggaaaaat ccagacgcgg aatgtggaag
     8161 ttgacgcgca atacagaaaa tatcgccgtt acccgattcc acagcatcag tatcgccgaa
     8221 aaactgcggc tgttcatcct ggaaccgcat acgggcaaaa cgcaccaatt gagagtggcg
     8281 atgaaaagtt tgggcagtcc gattctgggc gacagcttat acggcggaac agaatccgaa
     8341 accatgtttc tgtatgcatg gaaaatacaa tttgcctacc aaaaccgaca aatcgaaatt
     8401 gttgcaccct taaaaaacga atggcagact gaaaatatct ctcatgcgct ggaagaattt
     8461 tatatggcaa aagcaggtta ataaatataa attaatttat aaacaatatg ataaatattt
     8521 ttataaaaat ttttcaagaa aacggttgac tgcaaatctc ttca
//
LOCUS       DAWKOD010000078         8435 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000078 DAWKOD010000000
VERSION     DAWKOD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.078"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..653)
                     /locus_tag="WJK73_001316"
     CDS             complement(27..653)
                     /locus_tag="WJK73_001316"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ7557793.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAVDIIK
                     AQKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVDEAIRITGAEAIDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJK73_001317"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJK73_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ7557794.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WJK73_001318"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WJK73_001318"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ7557795.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WJK73_001319"
     CDS             complement(3056..3553)
                     /locus_tag="WJK73_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ7557796.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WJK73_001320"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WJK73_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7557797.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WJK73_001321"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WJK73_001321"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ7557798.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WJK73_001322"
     CDS             complement(5863..6309)
                     /locus_tag="WJK73_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ7557799.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WJK73_001323"
     CDS             complement(6314..6673)
                     /locus_tag="WJK73_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557800.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WJK73_001324"
     CDS             complement(6746..7474)
                     /locus_tag="WJK73_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557801.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WJK73_001325"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WJK73_001325"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ7557802.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
BASE COUNT         1654 a         2168 c         2446 g         2167 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcaactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 atcgcttccg ctccggtgat gcggatggct tcgtcgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccaacagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg atggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtggaact ggcggcagaa tgcgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggtacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg attttttgtg ctttaatgat gtcgacagcg
      541 cgggggcttt gggggtaaaa aaccagtccc aatgcgtctg cgccggcgtg ggcggcatac
      601 agtgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacggc gcgtttgtgc cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgtttaaaac
      781 cacttcgtcg ccttcacgcg ctttaatcag acaacgagcc agcggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacagaccga cggtcgcgcc gaaaaacact tggtcggtcg cttcgcgcaa
      961 ttcgggatca acgacgacgg cggcttccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag gtagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttattaa ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcggg ttgagtgctt tgacgacggc
     1501 ttccaaatcg cccaagtctt gaaacacgat gccgtccgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgcccattgg caatcaactc ttcgcgcgtg ggcatatcga tgccgtatac
     1621 gttgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgccagccgc
     1681 gcgtaccatt tcgacgattt cacggctggt cgtaccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctggccgg gcataataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 accttcgcgg taaggctttt tgaggtggac agcaagctcc atcgcgctgg ggcggctggt
     1921 gtcggggatg ggcatcacga catcgatgcc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccaaggac acgcccatat ccaagcgcga ctggtaaacg gacacgccgt caatcacgga
     2041 gtcgggacgg gcaaaataga cgtattcaaa aaggcagggg ctgagtttgg ccttatcgct
     2101 gcattgtcgg gcgatgattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 atcgcgttcc aaatcgtagg caagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggatttcctg ccttcgctgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacaccgt atgcgccgtg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactt cgcggcgcaa ttcgtgcgcg aatacgttga gcaagacttc
     2461 ggaatcggag ctggtgttga cgtggcgcag gtgtttgttg cacacgtttt cataaagttc
     2521 ggccgtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaacggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgcccgc gttgcccgca gtaggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgtgtg cggaacactt cgcgcaccat
     2701 gcctttgcct ttgtgcatat ggaaggtacc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagagaat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccgg agtttccgcc gtgccgccgc tatggttcaa cacggcttcg gaaagcgata
     3121 cgaaaaacgg cagtgtgtaa gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca aacattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgctgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaggtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ctgtctgaaa
     6301 ggcgcgcacg gcgttacatc atattgtcga acacgggcgt gatgttcaaa tccgcttctt
     6361 ccatatccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttgtcga tgtcccagcc ggcgagttcg gcttcaaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggttcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggctat gccgtctgaa
     6721 cgttcggttt tcagacggta tagcatcagt gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat ggtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgctgct gctggccgcc ggaaagttgg gacggaaaag
     7081 cgtctttttt gtgcgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctccgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt gatgttttcc aatacggtca
     7201 ggtgggggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcgtca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gccacatatt tctccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tgctcgaaat cgtccgcctt gccgatacgg
     7861 tgtttcaccg gaatcatgcg gacgacggcc gaatccactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgatgatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttcc
//
LOCUS       DAWKOD010000079         8407 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000079 DAWKOD010000000
VERSION     DAWKOD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.079"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJK73_001326"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJK73_001326"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ7557803.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="WJK73_001327"
     CDS             complement(1434..2039)
                     /locus_tag="WJK73_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557804.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJK73_001328"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJK73_001328"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ7557805.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="WJK73_001329"
     CDS             3024..3320
                     /locus_tag="WJK73_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557806.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGFYVPDCRKSSLSPKNI"
     gene            3334..4002
                     /locus_tag="WJK73_001330"
     CDS             3334..4002
                     /locus_tag="WJK73_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7557807.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WJK73_001331"
     CDS             complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WJK73_001331"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ7557808.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="WJK73_001332"
     CDS             complement(6432..7295)
                     /locus_tag="WJK73_001332"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ7557809.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7494..8357
                     /gene="rfbD"
                     /locus_tag="WJK73_001333"
     CDS             7494..8357
                     /gene="rfbD"
                     /locus_tag="WJK73_001333"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ7557810.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAAHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2008 a         2425 c         1944 g         2030 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atctgtactg tctgcggctt
      121 cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgtg
      181 gcggcaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcgaca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgttt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagttccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg cacctgcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc cccgttgccg cactgcccga cctcgctgcc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa acggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcacccga aacataccgg caaacccggc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgatg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ccgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat tctatgtgcc ggactgccgg aaatcatccc
     3301 tttcaccgaa aaatatctga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgct tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttagg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgcc
     4261 cggcatcaca ctgacaatcg cgccgacatt gttgtcgagg attttcacca cagtgccttc
     4321 gtacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggttg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtgaa caagcgtggc gcgtgtgcgg acagctcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 cgccaaagcg atttgcataa tctctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccagccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa cttattgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcggac agcaggctca agcagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt ggattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 accgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaactt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggctttggaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttacata accgacgcgt gccgcgccga tcgggccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatatcaga
     5821 atcgatttca ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcagggaac agcggacgaa tcggacggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccgggaatt ttgcctgcgg cgtaagtgcg
     6001 ctcgaggtaa tcgacagtca gagggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg ccgtattgga aagttttaac
     6181 gtgtttgtcg aacattattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccaaat tataaaggca accatcatta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gactttgtat
     6601 tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat
     6721 ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt tggcgcgcac ttccgccgcc
     6781 aattcttttc cgataatgcg ttcgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggttgaggg cgtaaaaatc acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcaggca gttgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccacgcac aatcgcctct actttcaccg gagtgagctt tttagccacg
     7021 acggcgcgtt tctctaaagc tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcacc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcgaa tgcagacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccaaaataga tctttaccaa accaatctca ctcatttcgc cccccctgaa aatatcttga
     7321 aaataccgac ccgacacccg acaggtttga atcacaaacc aatattctag ccgaagtcgg
     7381 cgcaaaacaa tacccatggc acaaaaaacc aacccgtcaa ccgtcggcaa agttttggca
     7441 ctataatacc gacagcaagt cctacaatac acttttacca aaggaaatac ctcatgagaa
     7501 tcctattgac aggctcaaaa agccaactgg cacgctgcct gcgcgaccgt cttccggaag
     7561 actgggaaac cattgcgacg gattccgcat ccctagacat taccgatgcc gatgccgtct
     7621 gcaacatggt caaaagtttc caacccgacg ctattgtcaa cacggctgcc tatactgccg
     7681 tcgacaaagc ggaaggcaat gcggcagcgg catttgccgt caatgcttcc gccgtttaca
     7741 accttgcctt ggcagcacat cgcgcccatg cccgattcat ccacatctca accgactata
     7801 tctttgacgg taaagggaaa aggccctatc aggaaagcga ctttaccaat ccttccaatg
     7861 tatacggaca atccaaaacc gcaggcgagc tgctcgcact gtctgccaat cccgacagcc
     7921 ttatcctgcg gacttcttgg ctgtttagcg aatacgggga caactttgtc cgcaccatgc
     7981 tgaaccttgc gcgggaacgt tccccgctgt ccgccgccca caaccaaatc ggctgcccga
     8041 cctatgccgg cgatttagct gccgccatca tccgcctttt gcagcaaacg tcgcccgtgc
     8101 gcggcattta ccactacgcc ggcggcaaat ccgtatcctg gtacgaattt gcccaacata
     8161 ttttccaagc ggcatcgcaa cagcagacat ccttccccgt tcccgaattg actgccgttt
     8221 cagacaagga atatccgacc gccgccccca ggcccgcata cagcattttg gactgccgca
     8281 aaatcgaaaa cgacttcagc atcaaaccgt cagactggca aaaagccctt gcacaggtcg
     8341 tttccaagct gctctgatgc cgcccgcctc tccgtttccg ccgtcaagca ccgccttggc
     8401 ggttttc
//
LOCUS       DAWKOD010000080         8389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000080 DAWKOD010000000
VERSION     DAWKOD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.080"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(354..629)
                     /locus_tag="WJK73_001334"
     CDS             complement(354..629)
                     /locus_tag="WJK73_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7557811.1"
                     /translation="MNASNWSVYLILCENSAFYCGISTNPQQRLDAHTAGKGAKYTRV
                     FKPVEMRIVAGGMDKGTALRQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(626..1909)
                     /locus_tag="WJK73_001335"
     CDS             complement(626..1909)
                     /locus_tag="WJK73_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7557812.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFVLALPGMLLLLKVAPWNGGKTQDTGR"
     gene            complement(2063..3481)
                     /gene="glnA"
                     /locus_tag="WJK73_001336"
     CDS             complement(2063..3481)
                     /gene="glnA"
                     /locus_tag="WJK73_001336"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ7557813.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3792..4601
                     /gene="aroE"
                     /locus_tag="WJK73_001337"
     CDS             3792..4601
                     /gene="aroE"
                     /locus_tag="WJK73_001337"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ7557814.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4603..5304
                     /gene="mtgA"
                     /locus_tag="WJK73_001338"
     CDS             4603..5304
                     /gene="mtgA"
                     /locus_tag="WJK73_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ7557815.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5628..5703)
                     /locus_tag="WJK73_001339"
     tRNA            complement(5628..5703)
                     /locus_tag="WJK73_001339"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5668..5670),aa:Phe,seq:gaa)
     gene            complement(5894..6628)
                     /gene="lptB"
                     /locus_tag="WJK73_001340"
     CDS             complement(5894..6628)
                     /gene="lptB"
                     /locus_tag="WJK73_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ7557816.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6702..7232)
                     /gene="lptA"
                     /locus_tag="WJK73_001341"
     CDS             complement(6702..7232)
                     /gene="lptA"
                     /locus_tag="WJK73_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ7557817.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7213..7794)
                     /gene="lptC"
                     /locus_tag="WJK73_001342"
     CDS             complement(7213..7794)
                     /gene="lptC"
                     /locus_tag="WJK73_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ7557818.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7791..8327)
                     /locus_tag="WJK73_001343"
     CDS             complement(7791..8327)
                     /locus_tag="WJK73_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7557819.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1972 a         2265 c         2159 g         1993 t
ORIGIN      
        1 attatggtag atactaactc tttaagttga catattgtgt gatatcgagg gctgtaccgt
       61 attttcccat cgtattttcc cgttctttat atgagcggca ggcatcctca taaaagaagg
      121 gataccgata aggaaaaata ccccggcata aaaaatatac tgattaatca atacaaatcc
      181 aacaaacagt tcatcgtatt cttttctgtt ggcgtataaa aaatactgat atatgccttc
      241 actttcatag ggcgacgctt tatgtaccgt cctgtgtgtt gaaacgtcgg ttttccttac
      301 cgcctgcccc gcttttccct gattgctgcc aactgccgtc cgaacctgaa ccgtcaggtt
      361 tcagacggca ttttttctgc ctgttcccac aatttccgtt tttgtgcggc ggtcagtttt
      421 ttgacggcga tttcctgcct gagtgccgtg cctttatcca tcccgcctgc aacgatacgc
      481 atctccaccg gtttgaatac gcgggtatat ttcgcgcctt ttccggccgt gtgggcgtca
      541 agccgctgtt gcggattcgt gctgatgccg caatagaacg cgctgttttc acacaatatc
      601 aggtaaacgc tccagtttga cgcgttcatc tgcctgtatc ctgagttttt cccccgttcc
      661 aaggcgcaac tttcagcaac aacagcatac ccggcagggc aagtacgaaa cacaactgga
      721 aaaacggtac ataaccgagc cattcgatca gataaccggc aaaggaattg atgaccgtac
      781 ggggaacagc ggacaggctg gtaaacagtg cgagctgcgt tgcggtaaat gcgggattgg
      841 tctcgcgcgc catatacgat acgaacgccg ccgtccccaa acccacgccg actgcctctg
      901 cgccgataac tgccgccagc atcagcctct cgcctgtgcc gaccgtgtcg aaatgtccga
      961 accctgccag ccatacaaac cccaaaacgg ttacagcctg caccgcgcca aatagccaca
     1021 aggctttgtt tacgccgatt ttcagcatcc acacgccgcc caagatacct gccgccactg
     1081 ccggccacag tcctgcattt ttcgcaatca aaccgatgtc tgtcttgctg aaacccatat
     1141 ccagataaaa cggcgttgcc aacgcggttg ccatactgtc gccgagtttg taaaggaaga
     1201 taaacagcag cacgcacacc gccgaagcga tgcccttgcg cgtaaaaaat tctttaaacg
     1261 gctctaccac ggtctgcttc aacgttttag gaacggcagg cggcaacacg ggttcgcgcg
     1321 caagaaacag cgtcatcaga aggccgggca gcataaataa tgaagtgata acaaatactt
     1381 ctgaccacgg catcctgtct gccaacacca aactcaatga accgggaatc agggcggcaa
     1441 cccgataggc gttcacatga accgagttgc ccaaacccaa ttcttcgtcc gacaaaatct
     1501 cgcgcctgaa cgcatccaat acaatgtcct gactggcgga aaaaaaagcg acaagcaccg
     1561 acaagccggc aatcagcggc agatgattac gggggtttaa aaaggcatag gccgccaaag
     1621 ccgccagcaa ccctgcctgc gtcagcagca tccacccgcg ccgccgtccc aaaacgggca
     1681 gcctgaccgc gtccatcagc ggcgaccaca aaaatttcca agtaaacggc agaccgatta
     1741 acgccatcag cccgatgctc ttcaaatcca cctgctcgct gcgcaaccac gccggaatca
     1801 ggttaatcag aaagtacagc ggcagccccg aggtaaaccc cgtaaaaata caaacaagca
     1861 tatttcggga aaagatttgc ccgataaaac ctgatttcga tacagtcatg gttcaaacag
     1921 ccaagaaata aaagcctatt gtagcaaata tcgcccaacc gctgaaaaac accgcccctg
     1981 ctccgccgtt ttgccgttca aatgcaaaat gccgtctgaa actgtgttca gacggcattg
     2041 cttttctgaa aacccgacgt gcttacaggc tgtaatacat ttcaaattcc agcggatgcg
     2101 gtgccatgcg gatgcggcgg acatcttcct ctttaaaggc gatatagctg tcgatccagt
     2161 ctttgctgaa cacgccgccg cgcaggagga attcgtggtc ggctttgagg gcggcgaggg
     2221 cttcttccaa agaggcgcaa acggtcggga cgagcgcgtc ttcttccggc ggcaggtcgt
     2281 acaggttttt atcggcagga tcgcccggat ggattttgtt ttgaataccg tccaaacccg
     2341 ccatcaacag ggcggcaaat gccaaatacg ggttggcggt cggatcgggg aaacgcgctt
     2401 cgatgcggcg cgccttgctg ctgttcacgg acggaatgcg gatggaagcg gaacggtttt
     2461 tggcggaata tgccagtttg gtcggtgctt caaagtgcgg cacgaggcgt ttgtaggagt
     2521 tggtggacgg attggtaatt gcgttcaggg ctttggcgtg tttgatgatg ccgccgatgt
     2581 agtagagcgc ggtgtcgctc aagccggcat agccgtcgcc tgcgaacagg ttttgaccgt
     2641 ctttccaaat ggattggtga acgtgcatac cgctgccgtt gtcgcccata atcggtttgg
     2701 gcataaaggt ggctgttttg ccgaagttgt gggcaacgtt ttgaatcaca tatttcatgt
     2761 cttgggtttg gtcggcgcgt ttgaccaagg tggcgaaacg cgtgccgatt tccatttggc
     2821 tgccggtacc gacttcactg tggtggactt cgacttcgat gccgagtcct tccaaaatgt
     2881 ttaccatcgc ggaacgcaaa tcctgaccgc agtcaatcgg cgcgacgggc gcgtagccgc
     2941 ctttgacggc gggacggtgg ccggtgtttt gaccgtccat atgcaggccg cttgcccacg
     3001 cgccgctttc ggacgtgatt tcgtaacggg ttttgtgcat gtcggtttca aattctacgc
     3061 cgtcgaagac gaagaattcg ggttcggggc cgaagtatgc cgtgtcgccg atgccggaag
     3121 atttcaaata ggcttcggcg cggcgggcga tggagcgcgg gtcgcggtcg taaccctgac
     3181 cgtcggcagg gtcgatgacg tcgcaggtaa tgacgacggt aacatcatca taaaaaggat
     3241 cgacgaaggc tgtagacgca tcgggacgca actgcatatc ggaagcctga atgcctttcc
     3301 agccgccgat agacgaaccg tcaaacgcct gaccgttttc gaaccactct tcggggtctt
     3361 ccaacacgat gcgcgcaggc acggtaaagt ggtgctgctt gcctttggta tcggtaaagc
     3421 gcaaatcgac aaagcgggct tcgctttctt caatcaattt tacggcgttt ttaatagaca
     3481 tttttcagct cctggaaaag ggaaatagca aaatatacgg aacgcggcag attagcccaa
     3541 agccagcatg attctaccac gtccatgccg tctgaaactc gccctgcgtc aaatttcaag
     3601 ctggaaaata tagtttcaga cggcaagatt gtcaacaata tgcttcggtt tgtctgttca
     3661 aaagcagaat acccgccctt ttgtccgaat gccgacaaaa cagatgccgc ctgaaaacag
     3721 tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc ccgccttgat
     3781 ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc gtcgcccaca
     3841 gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac attgaatacg
     3901 aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt tttgaaacag
     3961 gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat ctggcggacg
     4021 agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt ctgaaaaacg
     4081 gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc acgcaggtca
     4141 aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc gcggtgcgcg
     4201 gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc aaccgcaccc
     4261 acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg atggcggatg
     4321 tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc ggtcagcttc
     4381 ctgccgtcag tcctgaaatt ttcctcggct gccgccttgc ctacgatatg gtttacggcg
     4441 acgcggcgca ggagtttttg aactttgccc aaagcaacgg tgcggccgaa gtttcagacg
     4501 gactgggtat gctggtcggt caagcggcgg cttcctacgc cctctggcgc ggatttacgc
     4561 ccgatatccg ccctgttatc gaatacatga aagccatgta atatgttccg catcgtcaaa
     4621 tggctgattg ccctgcccgt cggcatcttt atctttttca atgcctatgt gtacggcaac
     4681 atcattacct accgcgccgt cgcgccccat cagactgcct ttatgtcgat gcggatgaag
     4741 cagtttgaac aggaaggtcg cgatgtcgca ctggattacc gctggatgcc ctacaaccgc
     4801 atctcggtca acctgaaaaa agccctgatt gcttccgaag atgcccgttt cgccgggcac
     4861 ggcggcttcg attggggcgg cattcaaaac gccatcaggc gcaaccggaa cagcggcaaa
     4921 gtgaaggcgg gcggctcgac catcagccag cagcttgcca aaaacctctt cctcaacgaa
     4981 agccgcagct atatccgcaa aggcgaagaa gcggcgatta ccgcgatgat ggaagccgtt
     5041 accgacaaag acaggatttt tgaactgtat ttaaactcaa tcgaatggca ctacggcgtt
     5101 ttcggcgcgg aagccgcgtc ccggtatttt tatcaaatac ccgccgccaa gctgaccaaa
     5161 cagcaggcgg caaaactgac ggcgcgcgtc cccgccccgc tctactacgc cgaccatccg
     5221 aaaagcaaac ggctccgcaa caaaaccaat atcgtgctca gacgcatggg ttcggcagag
     5281 ttgcctgaaa gcgacacgga ctgattgttt cagatatgga aatgccgtct gaactgcggt
     5341 tcggacggca tatgttttct ggtttttata gtggattaac aaaaatcagg acaaggcgac
     5401 gaagccgcag acagtacaaa tagcacggaa tcgattcact tggtgcttca gcaccttaga
     5461 gaatcgttct ctttgagcta aggcgagcca acgctgtacc ggtttaaatt taatccacta
     5521 tacctgcccg acagccgcaa agaccgtatc atacatagca ggcattgcgg tgcgggatta
     5581 ctgccgcaaa cggcaggcaa aaaaataacc gcttcaaggc ggttttttgg tggccagagg
     5641 cggaatcgaa ccgccgacac acggattttc aatccgttgc tctaccaact gagctatctg
     5701 gcctttgtct gtctcgttaa gagatgcgta ttaaaccaaa ttacgggacg acatgcaagc
     5761 atatcgggca atattctttg aaaacaggtt taagtcaatg ttttaaattt gaattaattt
     5821 atgtaaaaac aacataaatc cgtatttttg ccgcctgccc gacgatatta ggtcgcccgt
     5881 ctgaaaaata ttttcaatat ttgaagttct tacccagata aacagaacga acctgttcgt
     5941 ttccgaccaa atcatcaggt tttcccgatg ccaacaccgt gccgtctgaa ataatgtagg
     6001 cccgatcgca gatgctgagg gtttcgcgta cgttgtggtc ggtaattaat acgccgatac
     6061 cgcgcgactt gagaaatccg atgatttttt ggatgtcgat gacggcgatg ggatcgacgc
     6121 cggcaaaagg ctcgtccaac aaaataaaat gcggtttcat ggcaagtacg cgggcgattt
     6181 cgacgcgtcg gcgttcgccg cccgacaaag atggggcagg attattgcgc aaacgctcga
     6241 tattcaggtc ggcaagcagt ttttcaactt cgccgtcgat gcggcttttg tctttggtac
     6301 tgatttctaa aatggcgcgg atgttttgtt cgacggtcat tttacggaaa atagaggctt
     6361 cctgcggtag gtagccgaca cccaggcggg cgcgttcgtg tatgggcagg tgacgcaatt
     6421 cctgcccgtc cagcatcacg ctgcccgcat cggcggcaat gagtccaaca atcatgtaga
     6481 agctggtggt tttacccgca ccgttgggcc cgagcaggcc gacgacttcc ccgctttcga
     6541 tttcaaggga gaagctttta acgacttggc gttttttgaa acttttttgc aggttttgaa
     6601 caacaaggcg gctgacgttt gcgctcataa atatctcttc aatatcttcg gtcggcaaat
     6661 gccgtccgaa ccaggtttat gtttcagacg gcattttggg attattcgga tttttgcgta
     6721 ctcgaaggct ggataacgac gctgaccctg ccggatttgg aagcggattt tgcgccggat
     6781 tttgtgctgc cgctgatggt atagacttcg gttttggtgt tgtatgtaat caccgcacct
     6841 tcggcgacat cgccgccgcg ctgtactttg gcattaccgg ttaagactac ggtgctgcct
     6901 gcagatgaat aagcaacgtt gtttgcctgt ccgcgcaccg tgcctttgcc gccgtccaat
     6961 gtctggctga agcggactgg cgaaccttcc gccctcacgg attcgccgcc tttgccgccg
     7021 cgtgtaacat tgacgcgggc ggcggaaata ttgagcgtac cctgtctgat gacgacgttt
     7081 ccgctgaatg tggtgctttg gttggcttga tcgagcgaac cttggtcggc ctcaatctga
     7141 ataggctgcc tgctgtcgct ttgaagggca aaagcggggg acgccgaaaa aaatgctatt
     7201 aaaacaaata gcttacatat cttttgtatc ataaatcgtg gctttcactt tagatgagaa
     7261 gttcaacatg cctgttttgt ggtcgtaagt catgccgccc gcctgaccgt gcgatgcacc
     7321 atattggaaa ctgacaggcg tatcggtttg ggcatattga gattcggtat cgacgtgcag
     7381 cttttcggct tcaactttac ccgcctgccg tttgccgtcg gcggttttgg tcagcacaac
     7441 gttgttttta aaaagaacct gtttgttttc ggtatggtaa acggcttcat cgctgccgac
     7501 ttcgtacaac aacctgcctt cttggaagaa cacgagatgc ggtgagtcga aatgaatgtc
     7561 gctgctttcg ggaaactgtt tcgcgccctt cgaactcaaa tgttctttca agtatccctg
     7621 ttcgtcaaac cgcctgccat ccaatccgtc cattgtgtat tgcggttcgt cgggattgag
     7681 cctgacttct tcaatctcga cttcgctgat gcgtcccaac catgccgaca ggctgcccaa
     7741 ggcaaccgcc aatatcaatg ggaacgcaat tccgtaccgc catcttactt tcatttgatg
     7801 tactcgttca aagccgcgcc caaagtccct tgcgcctgca taatcaggtc gcacacttcg
     7861 cgcaccgcgc ccgcaccgcc ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac
     7921 caatgcgcgt cggggacggc aaccggcaat ccgcagcgta ccataacggg caaatcgacc
     7981 acgtcgtcgc cgacaaaggc acactcggct tcttccacgc cgacctgagc gcgcagctct
     8041 tcatacgcgg cgcgtttgtc cgaaatgcct ttgaagtagt aatttatgcc caactgtttg
     8101 acgcggatgc cgacggaggg cgcgtcccga cctgtgataa tcgcagtctg cacgccgctt
     8161 gcctgaagca ttttcagacc gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg
     8221 ttatcgcgga taaagatgcg cccgtcggtc aaaacgccgt ccacatccag gatcaacagt
     8281 ttgattttgg cggcgcgcgc ctgtaattcg ggagaaattg cctgcatggg atttccttgt
     8341 tttcagacgg catatcttac cacaaatgcc gtctgaaacg gctgcaacg
//
LOCUS       DAWKOD010000081         8298 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000081 DAWKOD010000000
VERSION     DAWKOD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.081"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            69..2183
                     /locus_tag="WJK73_001344"
     CDS             69..2183
                     /locus_tag="WJK73_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ7557820.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2320..3579
                     /locus_tag="WJK73_001345"
     CDS             2320..3579
                     /locus_tag="WJK73_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ7557821.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3947..5353
                     /locus_tag="WJK73_001346"
     CDS             3947..5353
                     /locus_tag="WJK73_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7557822.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5571..6785
                     /gene="gltS"
                     /locus_tag="WJK73_001347"
     CDS             5571..6785
                     /gene="gltS"
                     /locus_tag="WJK73_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ7557823.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6884..7900
                     /locus_tag="WJK73_001348"
     CDS             6884..7900
                     /locus_tag="WJK73_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7557824.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7911..8108
                     /locus_tag="WJK73_001349"
     CDS             7911..8108
                     /locus_tag="WJK73_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557825.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8126..8275
                     /locus_tag="WJK73_001350"
     CDS             8126..8275
                     /locus_tag="WJK73_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557826.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTRRTIYKAL
                     PYVPS"
BASE COUNT         2116 a         2420 c         1924 g         1838 t
ORIGIN      
        1 aaagacttgg caggcaaagt attggccgaa gcagtcacgt tttaagacta ttcaggccgt
       61 ctgaaagaat gttcctcttt tcagacggcc tacaatccat taataacaat aatcgacgaa
      121 aacgcattgt gaaaaacgcc tacatcccct ctcgcggcat ccgcaaaatc ccctatctct
      181 ccactctatt gcctgagttt catatctgca aggatgggaa aggagcagag gctgttatcg
      241 gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccaccgaa caccagcttc
      301 cctttattgc tttggaagac ggctttttac gctcgctcgg actgggcgtc tccggttatc
      361 ccccctactc catcgtctat gacgacatcg gcatctacta cgacactacc cgcccttctc
      421 gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg gctcaggcgc
      481 aacagacgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac gcgcccgaac
      541 tttcagacga tcatccttta cgctccccat ccaagcctga aactgtcctc attatcgacc
      601 aaaccttcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg tttgaactga
      661 tgtttcagac ggccttaaat gaaaacccgc aagccgatct ttgggtcaaa acccaccccg
      721 atgttttgtg cggcaaaaaa caaggctatc tgacccagct ggcgcagcaa caccgcgtcc
      781 atcttttggc tgaagacatt aatccgattt cgctgctgca agcggtggac aaagtgtatt
      841 gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg gtaacgtttg
      901 gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa atcaaccgcc
      961 ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc gcagcctatc
     1021 tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc tttgatgtca
     1081 tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag ttatattgcg
     1141 tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta ccctcttgcc
     1201 gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc gacgatgcac
     1261 gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa caacaccaca
     1321 tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc tccaacttag
     1381 tgccgccgct gtcgctcgtt accgacgata tgagcattta tttcaatgcc gaaaccccgt
     1441 cccgtcttga atacatccta caaaaccaaa acttcgacga tcaagacttt cagacggcct
     1501 tgaagctgca aaaaatgctg accgaaaacc acatcagtaa atacaacgtc ggcagctcag
     1561 acttcaccgc cccgtcaacc gacaaaaccg tgatcctcgt tcccggccag gttgaagatg
     1621 atgcgtctat ccgctacggt tcgccccaaa tctaccgcaa tctggatttg ctccgtaccg
     1681 tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat gtagtcagcg
     1741 gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac caaaccgccg
     1801 agcaaaccga catcctgacc tgtctccaat acgcagacga agtacatacc atgacttcgc
     1861 tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc ctgccttttt
     1921 acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc cgcagacttg
     1981 agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc caccccgaaa
     2041 cccatcaggc cataaatgca gaaaccgcag cccaaatcct gatacgacaa aaaaatatgc
     2101 aaaaaaacaa caacggatta catcgcgggt actttgccaa aaaattaggt aaaatcaaac
     2161 aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat aaacttgcct
     2221 ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt gaaagttcaa
     2281 cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac cgtcctcaaa
     2341 aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca aggccctgtc
     2401 ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac cgtacataaa
     2461 ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca taccgttgtt
     2521 tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac tcaacatcac
     2581 atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc aaaacgcatt
     2641 gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg cccctactac
     2701 atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg tgccgacttt
     2761 tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac gccggtacac
     2821 ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt gttccgcaat
     2881 ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca ttacctcaaa
     2941 ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat ccaaatcgcc
     3001 aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca ggtattcaac
     3061 gacagccaag tccgtatcca ttgcgacttt cccagtgtcc gcagcttcct gctccatgtt
     3121 ttgagttcat ttgccgaaca cgcgcctgcc gataccaaca tcatcatcaa gcatcatccg
     3181 atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa agaacacccc
     3241 gaactcaaag gccgcgtgat ttatgttcat gatgtccccc tgcccgtttt cctgcgccac
     3301 ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggcctgat tcacaatatg
     3361 ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga ccaaaatacc
     3421 ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgtttca tgcctaccgt
     3481 atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt gtttttcccc
     3541 aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg aaagttcagg
     3601 ccgtctgaaa acatttcaga cggcctgaaa caatcaatac cttagctact gccatgtaaa
     3661 taaaacacaa aaacctgcat ttatcattaa caataaatta caaaaacagt ataatgaccg
     3721 agctgtcatg agcgcatacc gactcaacct gagccctttg taacacacaa aatatggatg
     3781 tatccctagg caaaacaata taacaagcca aacatcctaa agataagccg gcaaggcaac
     3841 acactctaca aaaccgtgcc aagcaatatt tttacaaagc cctcaaccgg tatcgccgcc
     3901 catatgccgc agcatccgtc ttccacttta tatccgcccg caaaccatga ccgccgctcc
     3961 tgatatcctc taccggcaag ccgccgccct tttggaacaa tccaataccg cccaagccct
     4021 gcccctgctg caacaggccg cagagcaggg atatacggaa gctgcttttg tattgggcaa
     4081 ccatctgctg aaaaacggtc atccggagca ggcacttcca tggttggaag ccgccgcggc
     4141 ccaacgccat cccaaggcac tcttcaccct gctgcaacaa cgcgaacaca acggcacccc
     4201 gaccggacag cttcttaacg aatacgcctg gctgagtgag caaggtcacc tcgaagccca
     4261 attaattctc atgcgttacc acacgcagcg caacgatcca caatcgctct actgggcgga
     4321 gcttgccgct gcccaatatg ccgcacctgc gtattaccat ttggcacgcc atcatcaacg
     4381 ccaaggcgac gttgcgacag ccatcgaaca atacgaaaaa gcggcagcac tcggcataac
     4441 tgctgcctgc tggcaactcg gtcaaatcta cttctacggt acaggtgtca gccccaacca
     4501 cgcacaagcc gaacactatc tcgaacaagc ggcgcaagcc ggccacatcg ccgcacaaac
     4561 cctgctggcc gaccttcttg ccgcccaacg caaacccgaa gccttagaat ggtatcgtcg
     4621 tgccgccaat aaggagcaag cggaagcaca agccaagctg gcccaatacg ccctgaccgg
     4681 cgaactttcc gaacgcgatc cgttccaagc ggcgcgctat gccaaagccg ctgccgagaa
     4741 agaccatcct gaagccctga aaatcatggg cgacctctac cgctacggcc tcggcatcaa
     4801 agctgacaac catatcgcgc acgattacta ccaccgtgct gccgcgctgg gttctgccgc
     4861 cgcagcacaa aaactcatca gcgacgccgc gctgtaccat ccgcaacaat acgaacaaat
     4921 caaaactgcc gccctgcaac aacaacaaac cgaaaccatc taccgtttgg cggaagcaca
     4981 agcctgcgcc atcggccgtc ccgccgacta caatgccgcg cgaaaaaatt acatggaagc
     5041 tgccgggttc caccataaaa acgcagcggc agccttaggc cgcatctacc attacggcct
     5101 cggtacggcg caagatcctc gggcggctgc acactggtac gccattgctg ccgaacaaaa
     5161 ccacccttcc gcccaatacc acctcgcctg tttttactat cacgggcaag gtgtcggctg
     5221 tcatgttccg accgcctgct actggctgca ggccgccatc ggcaacggcc acacttcggc
     5281 cgaatcatta atatccctat tagaacaatg gcgacgcgaa gcacaccatg ccatcggaca
     5341 aaaggccgtc tgaaaagatt tacactcgca ttttttgaca atctttaact attcccctaa
     5401 tatttgccag ttatttttca cggacacgcc attgttttca tttctttctg aaaacacctt
     5461 gtccgcgcat caataccatg acactcggcg gataacgcca agcgttgaaa cacactacat
     5521 ccggaacaaa aacggatgct cggaaaaata tttctaggag gtgaaacaac atggaatggg
     5581 aattcaacag ttattacaca ctgattgccg ccacgctcgt gttgctggtt ggtaaatttc
     5641 tggttcaaaa aatcaaattc ttacgagact tcaatattcc cgagccggta gccggcggtt
     5701 tgattgccgc tatcgtcctg ttcgccctgc acgaggcgta cggcgtgagc ttcaaatttg
     5761 agaaaccgct gcaaaatgcg tttatgctga ttttcttcac gtccatcggc ttgagcgcgg
     5821 atttttcccg tttgaaggcg ggcggtttgc cgctggtggt ttttaccgcg attgtgggcg
     5881 gatttatctt ggtgcaaaac tttgtcgggg tcggactggc tacggctttg ggtttggatc
     5941 cgctcatcgg tctgattacc ggttcggtgt cgctgacggg cggacacggt acgtcaggtg
     6001 cgtggggacc taattttgaa acgcaatacg gcttggtcgg cgcaaccggt ttgggtattg
     6061 catcggctac tttcgggctg gtgttcggcg gcctgatcgg cgggccggtt gcgcgccgcc
     6121 tgatcaacaa aatgggccgc aaaccggttg aaaacaaaaa acaggatcag gacgacaacg
     6181 cggacgacgt gttcgagcag gcaaaacgca cccgcctgat tacggcggaa tctgccgttg
     6241 aaacgcttgc catgtttgcc gcgtgtttgg cgtttgccga gattatggac ggcttcgaca
     6301 aagaatatct gttcgacctg cccaaattcg tgtggtgtct gtttggcggc gtggtcatcc
     6361 gcaacatcct caccgccgca ttcaaggtca atatgttcga ccgcgccatc gatgtgttcg
     6421 gcaatgcttc gctttcgctt ttcttggcaa tggcgttgct gaatttgaaa ctgtgggagc
     6481 tgaccggttt ggcggggcct gtaaccgtga ttcttgccgt acaaaccgtg gtgatggttt
     6541 tgtacgcgac ttttgttacc tatgtcttta tggggcgcga ctatgatgcg gcagtattgg
     6601 ctgccggcca ttgcggtttc ggcttgggtg caacgccgac ggcggtggca aatatgcagt
     6661 ccgtcacgca tactttcggc gcgtcgcata aggcgttttt gattgtgcct atggtcggtg
     6721 cgttcttcgt cgatttgatt aatgccgcga ttctcaccgg ttttgtgaat ttctttaaag
     6781 gctgattttc cgcctttccg acaaagcacc tgcaaggttt accgcctgca ggtgcttttg
     6841 ctatgatagc cgctatcggt ctgcaccgtt tggaaggaac atcatgtatc ggaaactcat
     6901 tgcgctgccg tttgccctgc tgcttgccgc ttgcggcagg gaagaaccgc ccaaggcatt
     6961 ggaatgcgcc aaccccgccg tgttgcaagg catacgcggc aatattcagg aaacgctcac
     7021 gcaggaagcg cgttctttcg cgcgcgaaga cggcaggcag tttgtcgatg ccgacaaaat
     7081 tatcgccgcc gcctacggtt tggcgttttc tttggaacac gcttcggaaa cgcaggaagg
     7141 cgggcgcacg ttctgtatcg ccgatttgaa cattaccgtg ccgtctgaaa cgcttgccga
     7201 tgccaaggca aacagccccc tgctgtacgg ggaaaccgct ttgtcggata ttgtgcggca
     7261 gaagacgggc ggcaatgtcg agtttaaaga cggcgtattg acggcagccg tccgcttcct
     7321 gcccgtcaaa gacggtcaga cggcatttgt cgacaacacg gtcggtatgg cggcgcaaac
     7381 gctgtctgtc gcgttgctgc cttacggcgt gaagagcatc gtgatgatag acggcaaggc
     7441 ggtaaaaaaa gaagacgcgg tcaggatttt gagcggaaaa gcccgtgaag aagaaccgtc
     7501 caaacccacg cccgaagaca ttttggaaca taatgccgcc ggaggggatg cagacgtacc
     7561 ccaagccgca gaaggcgcgc ccgaaccgga aatcctgcat cctgacgacg gcgagcgtgc
     7621 cgataccgtt accgtatcac ggggcgaagt ggaagaggcg cgcgtacaaa accagcgtgc
     7681 ggaatccgaa attaccaaac tttggggagg actcgatacc gacgtgcaaa aagagttggt
     7741 cggcgaacaa cgcaagtggg cgcaggaaaa aatcagcaac tgccgacaag ccgccgcgca
     7801 ggcagaccgg caggaatacg ccgaatacct caagctgcaa tgcgacacgc ggatgacgcg
     7861 cgaacggata cagtatcttc gcggctattc catcgattag gggcaaaccg atgaataccg
     7921 tcccaaaaag caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag
     7981 tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg
     8041 tcagattggg cgaaaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg
     8101 gcagataaag agaaaattga cggaaatgcc gtctgaaacc ctgttatcgg tttcagacgg
     8161 cattttgacc aatacggtac gcaggcgcaa aacagccggc ttttcctgtg ttgcctatgc
     8221 tgatgtttca acacgcagga cgatatataa agcgttgccc tatgtgccgt cctgattcgg
     8281 aaagggttac gcccctcc
//
LOCUS       DAWKOD010000082         8286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000082 DAWKOD010000000
VERSION     DAWKOD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.082"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJK73_001351"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJK73_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ7557827.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WJK73_001352"
     CDS             complement(1659..2042)
                     /locus_tag="WJK73_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ7557828.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WJK73_001353"
     CDS             complement(2046..3587)
                     /locus_tag="WJK73_001353"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ7557829.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDVVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WJK73_001354"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WJK73_001354"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ7557830.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            4774..5997
                     /locus_tag="WJK73_001355"
     CDS             4774..5997
                     /locus_tag="WJK73_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7557831.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVAQASGMRRVAMLTLCGAIAGGGWWLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFASAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGMWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQSTAQKFPFIKRDNIKIIPLAVCMFALIGAIAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLNMPLTAAVFVLELTYAPAALLMPLCT
                     GMAGAVWTARKMGCK"
     gene            6161..7741
                     /gene="purH"
                     /locus_tag="WJK73_001356"
     CDS             6161..7741
                     /gene="purH"
                     /locus_tag="WJK73_001356"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ7557832.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDEVLSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1840 a         2275 c         2347 g         1824 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacatcac gctgttttcg ttgaagaaca gtgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 cacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcggggtt
      421 gacggtgtcg ttcgcaccga tgaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaaaacga tgtcgtaggg gactttggct tcggcgagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcgg tgccgttttt
      601 acgtaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacat tgtttgcgcc tttcagcatt tcggcaatat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat aatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 catgaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctacc gttaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gtttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc tcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 tttttaatgc cgtctgaact gttcagacgg catctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcccgcccac atcagcgagc gcaatgctgc tttgtctgcg
     1741 atgtagcacc agataaaggc gaggcggaac aggatgaaca ggccggcaag cgtgttgacg
     1801 gttgcttgtc cggcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgcgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataagcag gcaatcagga tgcaccaata ggcaaaagtc
     2041 atgctttatc ctttcctaaa catattcaac atgcggcgcg ttacgaagaa gccgccgaag
     2101 atattgatgc tggcaatcag gatggcgatg aaggcaagca gggtaacgaa accgttgcct
     2161 tggctgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggttaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggctgcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg cgccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtgatt
     3121 tggccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccacg
     3241 tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ctcgccttcg
     3361 gacggcgcat tgaccttata aatcaaaggg caggcccaaa ccgccgcttt gtcggcaacg
     3421 gtcgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg gggcagggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tttccgattg cagtcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagtt accgccatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga acctgattac
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cgaggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcgac
     4081 gaactcggtt taattgtcag cgatatggat tcgacgctga ttaccattga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaagtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 ttcttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcgggttc
     4381 acatttttta ccgaaaggct gcaacaacga ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggt cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc gcatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agcgcaggca gccgccgatg cctgtatcaa cttcggcggt
     4681 ttggagcgtg tacgcggcct gttcaggtag gcggatagga aacggatgcc gtctgaaagg
     4741 ttttcagacg gcatttgaac gtcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggttta gtcggcattg tgctgacaga actgatgcac
     4861 ttcatacagc atacggcata cggttatggc gcggacggtg cgtacatctc gttccgcgaa
     4921 ggtgtggcac aggcttccgg tatgcggcgc gtcgccatgc tgacgctgtg cggcgcgatc
     4981 gcaggcggcg gctggtggtt gctgaaagcc ttgggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaagc agccgttgca ggggctgccg tttctgacga cggttttcca tgttctgctg
     5101 caaatcataa cggtcggact cggttcaccg ctcggacgcg aagtcgcgcc gcgcgaaatg
     5161 accgccgcgt ttgcttcggc aggcggcaaa cgcttgggtt tggacgaagg cgaaatgcgc
     5221 ctcttgattg cctgtgcgtc cggcgcgggt ttggcggccg tgtataacgt gccgctcgcc
     5281 tccacacttt tcatcctcga agccatgctg ggcatgtgga cgcagcaagc cgtcgccgcc
     5341 gcgttgctga cttcggtcat tgccaccgcc gtcgcacgca tcggcttagg cgacgtgcag
     5401 caatatcatc ccgccaacct tgccgtcaac acctcattac tttggttttc cgccgtcatc
     5461 ggcccgatac tgggcgcaac cgccgttttt ttccagagta ccgcccaaaa attccccttc
     5521 atcaagcgcg acaacatcaa aatcattccc ttggccgtct gtatgtttgc actcatcggc
     5581 gcgattgccg tttggtttcc cgaagttttg ggcaacggca aagcaggcaa ccaactgact
     5641 tttggcggtt tgaccgactg gcaacacagc cttgagttga ccgccgtcaa atggctggtc
     5701 gtcttgatgg cgctggcggc aggcgcatac ggcggactga ttacgccgtc tatgatgctc
     5761 ggcagcacca tttcttttgc agccgccgcc gcgtggaaca gcgttttccc cgaaatgccg
     5821 tctgaaagcg cggcagtcgt cggcgccgcc gttttcctcg gcgtttccct caatatgcct
     5881 ctgaccgccg ccgtctttgt tttggaactc acctacgccc ccgccgcttt gttgatgcct
     5941 ttatgtacgg gtatggcggg cgcggtatgg acggcaagga aaatgggatg taaataaccg
     6001 aaggcaaaat gccgtctgaa accggttttc agacggcatt cctttgggga ttgtcaacaa
     6061 tatctgcaaa aacctactgc cggaaagtgg aaaatagcgg ataataccgc cccgacaaca
     6121 ttaccgtttc ccatcccaac gtcagaaaaa ggaaatccct atgccttcca tcaaacgcgc
     6181 cctgatcagc ctgtccgaca agacgggcgc agtcgaattt gcccaaaccc tgcacaagct
     6241 cggtgtcgaa atcctctcta ccggcggcac ggcgaagctg cttgccgatg cgggcgttcc
     6301 cgtgattgaa gttgccgact acaccggttt ccccgaaatg ctcgacggcc gcgtgaaaac
     6361 gctgcatccg aaaatccacg gcggtatttt gggccggcgc gatttgcctg aacacgtcgc
     6421 caagatggaa gaacacggca tcggcaacat cgacctcgtg tgcgtcaacc tttatccctt
     6481 cgccgccacc atcgccaaac caaactgcac actggaagac gcgattgaaa acatcgacat
     6541 cggcggcccg accatggtgc gttctgccgc aaaaaactgg aaacacgtcg ccatcgttac
     6601 cgacaccgcc gacttccccg ccatcgccgc cgaactcgaa gccaacaacg gcgcgttgag
     6661 cgacaaaacc cgcttcaacc tgtcgcgcaa agcgttcagc cacaccgccc aatacgacgg
     6721 catgatttcc aactacctga cctcgctttc agacgaggtc ttgagcggca cgcccgaaat
     6781 cggtgaattc ccgagccagt tcaaccaaag ctggattaaa gtgcaagaca tgcgctacgg
     6841 cgaaaacccg catcagcgcg ccgcgttcta ccgcgatatt gaccccgccg caggcagcct
     6901 ttccgcctac aaacaactgc aaggcaaaga attgtcttac aacaacatcg ccgatgccga
     6961 tgccgcatgg gaagccgtca aatcctttga cgcgccagcc tgcgtgatcg tcaaacacgc
     7021 caatccgtgc ggcgtcgccg ttgcagccga tacattgacc gcctacaaac tcgcctacgt
     7081 caccgacacc accagcgcgt tcggcggcat catcgccttc aaccgcgaag tggacggtga
     7141 aaccgtcaaa cagattaccg acaaccagtt tatggaagtc ttgatggcgc cgaagtttac
     7201 cgccgaagcc ctcgaaatcg ccgccgccaa gaaaaacgtg cgcgtattgg aagtgccgct
     7261 tgaggcaggc gcaaaccgct tcgaactcaa acgcgtcggc ggcggactgt tggtgcaaac
     7321 ccccgatatt caccgcctca accgcgccga tttgaaagtc gtttccaaac gccaaccgac
     7381 cgagcaagaa tggaacgacc tgatgtttgt ttggaacgtc gcaaaatacg tcaaatccaa
     7441 cgccatcgtc ttcggcaaag gcggtcaaac ctacggcatc ggcgcaggcc aaatgagccg
     7501 cgtggacagt acccgcatcg ccgcccgcaa agcgcaggac gcaggcttgg acttaaacgg
     7561 cgcatgtgcc gcctctgatg ctttcttccc cttccgcgac ggcgtggacg tgattgccga
     7621 gcagggcatc aaagccatca tccatccggc aggttcgatg cgcgatcagg aagtcttcga
     7681 cgcagcggac gaacacggca tcgccatggt cgtaaccgac gtgcgccact tccgccatta
     7741 acgcagatga acaaggcaat gccgtctgaa gggctttcag acggcatttt gcgctatttt
     7801 gtgaaggtag ggatgacggt tcgggtattc ctgacagggt ggattttcaa ggtgttgtat
     7861 agggtgtagg aggattcgta aaaggtggga tgcagggtgt gcttcagccc gctgcatcaa
     7921 aaatttttgg agaatcggcg ggagtcggcg gttttggttt cggcggggac ggtggaaatg
     7981 ggtaacattg acggaatcga cggaagcggt gggcttcagc ccaccgcttg tatattggaa
     8041 tcaccgtatc atagcgccac ccgcgcccac ccaaggcaca caaccgttgc gtagctcagg
     8101 gagcggcagg gcaacccatc gacacaaccg gacagttgcc ggacaacaca accgaatgca
     8161 aggcaggttt atgatgagta cccaatacca ttacgcaggt atagtgaatt aaatctaaac
     8221 cagtacagcg ttggttcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
     8281 aagtga
//
LOCUS       DAWKOD010000083         8248 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000083 DAWKOD010000000
VERSION     DAWKOD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.083"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            311..1480
                     /gene="prpF"
                     /locus_tag="WJK73_001357"
     CDS             311..1480
                     /gene="prpF"
                     /locus_tag="WJK73_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ7557833.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1894..3093
                     /locus_tag="WJK73_001358"
     CDS             1894..3093
                     /locus_tag="WJK73_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7557834.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGLDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3201..3821
                     /locus_tag="WJK73_001359"
     CDS             3201..3821
                     /locus_tag="WJK73_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ7557835.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVIDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3948..4391)
                     /locus_tag="WJK73_001360"
     CDS             complement(3948..4391)
                     /locus_tag="WJK73_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ7557836.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4565..4933
                     /locus_tag="WJK73_001361"
     CDS             4565..4933
                     /locus_tag="WJK73_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7557837.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4978..6132
                     /locus_tag="WJK73_001362"
     CDS             4978..6132
                     /locus_tag="WJK73_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7557838.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6189..7061)
                     /locus_tag="WJK73_001363"
     CDS             complement(6189..7061)
                     /locus_tag="WJK73_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ7557839.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7146..7958
                     /locus_tag="WJK73_001364"
     CDS             7146..7958
                     /locus_tag="WJK73_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ7557840.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLLALKYRVLDAV"
BASE COUNT         1781 a         2283 c         2379 g         1805 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaatt taatccacta tactgtaaat cgtcattccc
       61 gcgcaggcgg gaatccagaa agtggaattg aggaaacctt tttatccgat gagtttctgt
      121 gcggataaat ctggattccc gcctgcgcgg gaatgacggg gtttaataat ctgccgtatc
      181 acaacacagt agccgtagat tggggcgaac cccgacagtt tgcggaatca aacggctttg
      241 gtcggagtgg cagcctaatc cactataaaa atcgtgggca gagcccacgc tacataagga
      301 gaatctagaa atgccgcaaa ttaaaattcc cgccgtttac taccgtggcg gtacatcaaa
      361 aggcgtgttt ttcaaacgtt ccgacctgcc cgaggcggcg cgggaagcgg gaagcgcacg
      421 cgacaaaatc ctcttgcgcg tactcggcag cccggatccc tacggcaagc agatagacgg
      481 tttgggcaac gccagctcgt ccaccagcaa ggcggtgatt ttggacaagt ccgaacgcgc
      541 cgatcacgat gtcgattacc ttttcgggca agtttccatc gacaaacctt ttgtcgattg
      601 gagtggcaac tgcggcaacc tcaccgccgc cgtgggcgca tttgccatcg agcaaggctt
      661 ggtcgataaa ggcaagattc cttcagacgg catctgcaca gtcaaaatct ggcagaaaaa
      721 catcggcaaa accattattg cccatgtacc gatgcaaaac ggcgcagttt tggaaacagg
      781 cgattttgag ctcgacggcg taacgttccc ggcagccgaa gtacaaatcg aatttcttga
      841 tccagccgac ggcgaaggca gtatgttccc aaccggcaat ttggtcgatg aaattgatgt
      901 gccgaatata ggccgtttga aagccacgct catcaacgcg ggcattccga ccgttttcct
      961 gaatgccgcc gacttgggct acacgggcaa agagttgcaa gacgacatca acaacgatgc
     1021 cgcagctttg gaaaaattcg agaaaatccg cgcttacggt gcgctgaaaa tgggtctaat
     1081 cagcgacgta tccgaagctg ccgcccgcgc gcacacgccg aaagtcgcct tcgtcgcgcc
     1141 cgccgccgat tacaccgcct ccagtggcaa aaccgtgaat gccgccgaca tcgatttgct
     1201 ggtacgcgcc ctgagcatgg gcaaattgca ccacgcgatg atgggtaccg cctctgttgc
     1261 cattgcgacc gccgccgccg tgcccggtac gctggtcaac cttgccgcag gcggcggaac
     1321 gcgtaaagaa gtgcgcttcg ggcatccttc cggcacattg cgcgtcggtg cagccgccga
     1381 atgtcaggac ggacaatgga cggccaccaa agcggttatg agccgcagcg cacgcgtgat
     1441 gatggaaggt tgggtcaggg tgccggaaga ttgtttttaa attgacgtag catgggtttg
     1501 cccgcgagcc ataaacaggt cgtctgaaaa acaagtaaac atcaaatcac tgaccattcc
     1561 tttcccttgc cctgtggcgg aaggcggcaa atcacaagga agaacacgga aaccccgata
     1621 aaagacagct tcccgtatta ccgtcattcc cgcgcaggcg ggaatctaga cctgtcaata
     1681 tggaggattg gtaggggaaa acaggtttcg tgagttctac attctggatt cccgccacag
     1741 cctgtcctcg cgtaggcggg gacggaataa cgatagaaaa tgcggcatac gctttgccca
     1801 aagaggccgt ctgaaacacc ttgcgcctga tgtctgcctt tttcagacga ccccacacca
     1861 aaaaaacaac cacaaactac aaggagaaac atcatgtccg accaactcat cctcgttctg
     1921 aactgcggca gttcatcgct caaaggcgcc gttatcgacc gcaaaagcgg cagcatcgtc
     1981 ctaagctgcc tcggcgaacg cctgaccacg cccgaagccg tcattacgtt cagcaaagac
     2041 ggcaacaaac gccaagttcc cctgagcggc cgaaattgcc acgccggcgc ggtgggtatg
     2101 cttttgaacg aactggaaaa acacggtctg cacgaccgca tcaaagccat cggccaccgc
     2161 atcgcccacg gcggcgaaaa atacagcgag tctgttttga tcgaccaggc cgtaatggac
     2221 gaactcaatg cctgcattcc gcttgcgccg ctgcacaacc ccgccaacat cagcggcatc
     2281 ctcgccgcgc aggaacattt ccccggtctg cccaatgtcg gcgtgatgga tacttcgttc
     2341 caccaaacca tgccggagcg tgcctacact tatgccgtgc cgcgcgagtt gcgtaaaaaa
     2401 tacgctttcc gccgctacgg tttccacggc accagtatgc gttacgttgc ccctgaagcc
     2461 gcacgcatct tgggcaaacc tctggaagac atccgcatga ttattgccca cttaggcaac
     2521 ggcgcatcca ttaccgccat caaaaacggc aaatccgtcg ataccagtat gggtttcacg
     2581 ccgatcgaag gtttggtaat gggtacacgc tgcggcgaca tcgacccggg cgtatacagc
     2641 tatctgactt cccacgccgg tttggatgtt gcccaagtgg atgaaatgct gaacaaaaaa
     2701 tcaggtttgc tcggtatttc cgaactttcc aacgactgcc gcaccctcga aatcgccgcc
     2761 gacgaaggcc acgaaggcgc gcgcctcgcc ctcgaagtca tgacctaccg cctcgccaaa
     2821 tacatcgctt cgatggctgt ggcctgcggt ggcgttgacg cactcgtgtt caccggcggt
     2881 atcggcgaaa actcgcgtaa tatccgtgcc aaaaccgttt cctatcttga tttcttgggt
     2941 ctgcacatcg acaccaaagc caatatggaa aaacgctacg gcaattcggg tattatcagc
     3001 ccgaccgatt cttctccggc tgttttggtt gtcccgacca atgaagaact gatgattgcc
     3061 tgcgacactg ccgaacttgc cggcatcttg tagccaaaaa agggacgagt ccgcaaaaat
     3121 gccgtctgaa attttcagac ggcattttgt ttggcgtttg ggcgtacaat acggaaaaca
     3181 tgacgataag gaaacaaacc atggcacagt ttttcgccgt tcatcccgac aacccccaag
     3241 aacgcctcat caagcaggcg gtcgaaatcg tcaataaagg cggcgtggtc gtttatccga
     3301 ccgattcctg ttatgccttg ggttgcaaac tcggcgataa ggcggcgatg gaacgcatac
     3361 tctccatccg aaaaatcgat ttgaaacacc acctgaccct gatgtgcgcc gatttgagcg
     3421 agttgggcac atacgccaaa atcgacaacg tacagtttcg tcagcttaag gccgccacgc
     3481 ccggacctta tacctttatc ttgcaggcga cgaaggatgt gccggcgcgc acgctgcacc
     3541 cgaaacgcaa aaccatcggg ctgcgtattc ccgataatgc cattgcacaa gccctgctgg
     3601 gggaattggg cgaaccgctt ttaagctgca ccctgatgct gcccgaagac ggcgaaccat
     3661 tgaccgatcc ttatgaaatc cgcgagcgtt tggaacacgc cgtcgatttg gtgattgacg
     3721 gcggctggtg cggaaccgag ccgaccaccg tcatcgacat gaccgacggt acggaattgg
     3781 tgcgccaagg ttgcggcgat acggcggtgt tcggtttgta gggaaaccga tgccgtctga
     3841 agcatcggct gttcagacgg cattgcgcgc cttgccggcg gcagtccgaa atgccggcgc
     3901 gtatcgcgct cggtcggaat atccgtttga aacggcattt tgatgcatta ctgcaccgca
     3961 atcggaattc tcggttcgta gagcaggtcg taggtcggct tgttgagcag gtcttggagc
     4021 gtgaaaccgt ccagatacgt gaaaaacgac ttcatcgcgc cgccgagtat gcccgtcagc
     4081 cggcaggacg gtgtaatcag gcattcgttg ttctcgccca tgcactcgac cagctgcatc
     4141 ggttcgaggt ggcggacaac cgagccgatg ttgatgcggt cgggcggtgc ggcaagccgc
     4201 agaccgccgc cttttccgcg cacactgtgg aggaagccgc ctttgaccag cgcggtaacg
     4261 accttcatca gatggctttt ggaaatgccg taggttacgg cgatggtact gatgttgacc
     4321 agcgcatcgt cgttgatggc agtgtagata aggacgcgca gcccgtagtc cgtatgttgt
     4381 gtcaaataca tgattttctc ggtatggatt gttattctta tcggtacggt ttaaggttca
     4441 cggacaatac cttaatggtt gaaaccctgt ccgtcggggc ggtagaatgc agcctgtctg
     4501 cggcggtatg ccgtctgaaa catccgcgct accgtttgat aatttgttat tgtaactcaa
     4561 aatcatgaaa ccgttgaaac gacatcccgc ccttatcggg ctttcgcgtg accaccacca
     4621 ttcgctttcc ctgtgcgtgc gtctgttgcg gacgccggaa gaaaggcatc gggacgaact
     4681 cgaaccgcat tttgccgaat tggaaaccca ttttcgcgaa gaagaagcca agtttgcccc
     4741 aatttggcag aatgtcgccc ccgaattgaa acaacgtttc gagaaagacc acgcccgact
     4801 gcggcagatg atggcaagcc ccgaatacgg taacgcggcg tggaataccg cttttgccac
     4861 aaccctgcgc gaccacgcgc gctttgaaga acgcgagctg tttcccgccg ccgaaccgtt
     4921 tttgccggca tgattccgtt ttgcggcaaa tatattaatg ataaacaagg aacacacatg
     4981 aaatttacca agcaccccgt ctgggcaatg gcgttccgcc cattttattc gctggcggct
     5041 ctgtacggcg cattgtccgt attgctgtgg ggtttcggct acacgggaac gcacgagctg
     5101 tccggtttct attggcacgc gcatgagatg atttggggtt atgccggact ggtcgtcatc
     5161 gccttcctgc tgaccgccgt cgccacttgg acggggcagc cgcccacgcg gggcggcgtt
     5221 ctggtcggct tgactatctt ttggctggct gcgcggattg ccgcctttat cccgggttgg
     5281 ggtgcgtcgg caagcggcat actcggtacg ctgtttttct ggtacggcgc ggtgtgcatg
     5341 gctttgcccg ttatccgttc acagaatcaa cgcaactatg ttgccgtgtt cgcgctgttc
     5401 gtcttgggcg gcacgcatgc ggcgttccac gtccagctgc acaacggcaa cctaggcgga
     5461 ctcttgagcg gattgcagtc gggcttggtg atggtgtcgg gttttatcgg tctgattggt
     5521 acgcggatta tttcgttttt tacgtccaaa cggttgaatg tgccgcagat tcccagtccg
     5581 aaatgggtgg cgcaggcttc gctgtggctg cccatgctga ccgccatgct gatggcgcac
     5641 ggcgtgatgc cttggctgtc ggcggctttc gcgtttgcgg caggcgtgat ctttaccgtg
     5701 caggtgtacc gctggtggta taaacccgtg ttgaaagagc cgatgctgtg gattctgttt
     5761 gccggctatc tgtttaccgg attggggctg attgcggtcg gcgcgtctta tttcaaaccc
     5821 gctttcctca atctgggtgt gcatctgatc ggggtcggcg gtatcggcgt gctgactttg
     5881 ggcatgatgg cgcgtaccgc gctcggtcat acgggcaatc cgatttatcc gccgcccaaa
     5941 gccgttcccg ttgcgttttg gctgatgatg gcggcaaccg ccgtccgtat ggttgccgta
     6001 ttttcttccg gcactgccta cacgcacagc atacgcacct cttcggtttt gtttgcactc
     6061 gcgcttttgg tgtatgcgtg gaagtatatt ccttggctga ttcgtccgcg ttcggacggc
     6121 aggcccggtt gagacaaacc gccgcagatt tcgggtctgg gcggtttgct tttcagacgg
     6181 cagggcggtc agttgccgtc cagccagcgg tcgcgtgtgg ttttggcttc ttcaaaatag
     6241 cggtacaggg cttcgcggtc gtcggtggtc aggatgtttg ccaaaacgtc caactgtttg
     6301 cccaagcctt gaaccagttg cagcaggctg tctttgttgg caaggcagat gtccgcccac
     6361 acggcgggat gaccggaggc gatgcgggtg aagtcccgaa agcccgtggc ggcgaatttc
     6421 agatattcct gtccgtcggg gtggtcgaga atctggtgga cataggcgaa ggcggtcagg
     6481 tggggcatat gggagacggc ggcgaaaacc gcgtcgtggc gttgcgcgtc catcgtataa
     6541 atttccgcac cgaccgcgtg ccacagttct tctaccaagg caatgccgtc tgaatgttcg
     6601 ccgccgtgtg gcgtgatgat gagttttctg tggcggaaca gcccgaactg cgcggcttgc
     6661 gcaccgcttc tgtccgaacc ggcaatcggg tgggcggcga tgcagcggtg caggcggtcg
     6721 ggcagacagc ggcggaaggc ttcgatgacc gaagatttgg tgctgccgac atcggaaatc
     6781 caagtgtgtt ccggcaaaac ggggcgcagc gcggtcaaaa tggcgggaac ggtggcgacg
     6841 ggcgtggcaa tcagtaccaa gtccgcaccg ccgatgctgt ccgcgtcgat ggcaacggac
     6901 gcacggtcaa tcacgccgcg ttccaatgca cgttcgaggt tgtcgcggtc ggtgtcgata
     6961 ccggtaacgg tgcggacgag tccctgcctt ttgaggtcga gaacgaacga accgccgatc
     7021 agccctacac cgatgagggc aatatggttc aaaatgggca tttgtgtaaa cggttttcgc
     7081 aaagtaccgt catggtagcc tatcggcgga atatgccgca agttcggcag gaaaaaggag
     7141 aagaaatgga caaaatcaga gttgccgccg tgcagatggt gtcgggcgtg tcgccggaaa
     7201 ccaacgtcgc tgccatgaaa cgcctggtcg cacgggcggc ggagcagggt gtggattggg
     7261 tgctgctgcc cgaatattgg gtgctgatgg gcgcaaacga taccgacaaa ctcgcgcttg
     7321 ccgagccttt gggcggcgga cgctttcaga cggcattgag cgaaacggcg aaagaatgcg
     7381 gcgtggtgct gttcggcgga accgtgccgc tgcaaagccc cgaggcgggc aaggtcatga
     7441 atactttatt ggtgtacggg cgcgacggca aacggacggg gctgtaccac aaaatgcacc
     7501 tcttcggctt ttccggtttg ggcgaacgct atgccgaagc cgataccatc cgcgcgggcg
     7561 gggatgtgcc gcacttgtcg gcagaaggcg tgtcggtggc ggcgggcatt tgttacgatg
     7621 tccgctttcc cgaatttttc cgccgccagc tgccgtttga cgtattgatg ctgcccgctg
     7681 cgtttacgca cacgacgggc aaggcgcatt gggagctgct gctgcgcgcg cgtgccgtcg
     7741 aaaaccaatg ttacgtcgtg gcggcggcac agggcggttt gcacgaaaac ggacggcgca
     7801 cgttcggaca cagcatgatt gtcgatccgt ggggcgacgt gttggacgta ttgcccgaag
     7861 gcgaaggcgt tgttacggca gacatcgatg ccaatcgcct gaacagcgtc cgcaaccgcc
     7921 tgctcgcctt gaaataccgg gttttggatg ccgtctgaag gttcagacgg catcggtgcc
     7981 ggggaatcag cgggctgtag gatgggcttc agcctgccat ttccatcaat cccacatttc
     8041 taccgttttc atcgaatcca tcgaatccgc ccctttcgac gacccagtcc tacgcaactg
     8101 aaccgtcatt cccacggaag tgggaatcta gaatctcggg gtttcagtca tttccgatag
     8161 attcccgccg cgtcaggggt ctggattccc gcttgcgcgg gaatgacgaa tccatccata
     8221 cggaaacctg caccacgtca ttcccacg
//
LOCUS       DAWKOD010000084         8236 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000084 DAWKOD010000000
VERSION     DAWKOD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.084"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WJK73_001365"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WJK73_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ7557841.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJK73_001366"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJK73_001366"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ7557842.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WJK73_001367"
     CDS             1963..2286
                     /locus_tag="WJK73_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ7557843.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            2333..2809
                     /locus_tag="WJK73_001368"
     CDS             2333..2809
                     /locus_tag="WJK73_001368"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ7557844.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="WJK73_001369"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="WJK73_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ7557845.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WJK73_001370"
     CDS             complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WJK73_001370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ7557846.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4772..5377)
                     /locus_tag="WJK73_001371"
     CDS             complement(4772..5377)
                     /locus_tag="WJK73_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557847.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5378..5602)
                     /locus_tag="WJK73_001372"
     CDS             complement(5378..5602)
                     /locus_tag="WJK73_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ7557848.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5711..6322
                     /locus_tag="WJK73_001373"
     CDS             5711..6322
                     /locus_tag="WJK73_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ7557849.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERARRYAPSIKPHDK"
     gene            complement(6382..7254)
                     /locus_tag="WJK73_001374"
     CDS             complement(6382..7254)
                     /locus_tag="WJK73_001374"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ7557850.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7291..8076)
                     /locus_tag="WJK73_001375"
     CDS             complement(7291..8076)
                     /locus_tag="WJK73_001375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ7557851.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
BASE COUNT         1983 a         2493 c         1966 g         1794 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacctgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttatcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acgcggtcgg catcggtaaa acgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccgagtgcct cggtaacgtt ttgattcaaa cggtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaaaccgg gcgtatcgac aaacacaaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtaatac
      901 tgattttctg accgatgaga tgattcatca gcgttgattt gccgacattc ggacggccga
      961 caatcgctac gaagccgcaa cggtatccgt cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat atttgctttc gtatccatat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgct
     1141 tcctgctccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaatgacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aggcgaaacg gcgcgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc gactcgccgg acgcgttctg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc cgcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgctgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatcg ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcaagaa
     1921 tcgtggtaaa atccgcaagt tttcccccaa cgtattcccg caatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgcatc gccaccaaca ccgtcgccaa acaaaaccag
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgacccgg acaacttcag catcgtcagc
     2161 ctcgccgata tcaccaaaac caaccctgaa accgccgcac tgctctccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaacctaag
     2281 gactgaccct tcagacggca ttccgaaacc tccgaatacc aaaggacact caatgaacac
     2341 catcgccccc aacctcgacg gcaaacacct ccgcatcggc atcgtacagg cacgcttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacttt gcctcttctg aaaaatttga cgcactgatt gccatcggcg tcgttatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcagggg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accacggaaa acgacgcaca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tagaatgcgc
     2761 caacctcgtc aacctcctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccgata cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgag attgctaaaa acatccgcga aatgcccgac
     3001 tttgccaagg cagacgaaga attgttcaac aaacttttct tcggcacgca aaccaatgcg
     3061 gcagagtaca tccgacaaat ccgccccctg ctcgaccgcg acgaaaaaga cctcaacccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca tcatcaacga agccatcgaa gtaaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgtcc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca ggtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct ggtcgcatcg aatttccgga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgttgacgt atcaatcagt caacaacaca aatgccgtct gaaaaacctt tcagacggca
     3661 ttttgtttcc agccccatca atactgtacc gtccagccga tttcgccgcc cgcgcgcatc
     3721 gggacaagtt cgccgtcgcc gtagggaata cttgcgggga cggtttggct ttgtttgacg
     3781 agggtgatcg tgtcggtatt ttcaggaatg ccgtaaaacc ttgcgccgtt tttcgaggcg
     3841 aaggcttcga gtttgtccaa cgcgcctgct ttttcaaaca cttcggcata aagctcgatg
     3901 gcggtcatcg cgctgaacat accggcgcaa ccgcaggcgt tttctttggc ggatttggcg
     3961 tgcggcgcgg agtcggtgcc gaggaagaat ttatgcgcct tctcgccggt aacggcggca
     4021 accaatgcct gacggtgggt ttcgcgtttg agtacgggca ggcagaaatg atgggggcgc
     4081 acgccgccga ccaagaggtc gttgcggttg agcaggaggt gttgcggggt aacggtggcg
     4141 gctacgttgt cgcctgcttc caaaaccagg cgggcggctt cggcggtggt gatgtgttcg
     4201 aacacgactt taagattcgg cacttgcgcc aaaacgggtt tcatcacgcg cccgataaag
     4261 gcggcttcgc ggtcgaagat gtcgatttcg gggtcggtta cttcgccgtg aacgaggaac
     4321 aaaatgccct gtttcgccat ttcttccaac acggggatga gcttgaacag gtcggttacg
     4381 ccggaatcgg aattggtggt cgcgccggca gggtagagtt tgaaggcgac gatgccggcg
     4441 gctttggctt cgcgcacaag tgcaggcgtg gcttggtcgg tcaggtaaag cgtcatcaac
     4501 ggctcaaacg cgctgccttc gggcaatgcc gccatgatgc gcgctttgta ggcaagcgcg
     4561 tcggcaacgc tgacgacagg cggtttgagg ttgggcataa tgacggcacg ccccatttgg
     4621 cgggcggtat aaggagcaac ggctttgagc gcgtcgccgt cgcgcaggtg caggtgcata
     4681 tcgtcggggc ggataatggt cagggtttgc atggttttcc tttgttttat ttgaatgttc
     4741 agacggcatg ccgactttat gccgtctgaa gttaggggag aattaggggt ttcccgttgt
     4801 gggaacggtg cgtcaggttc aaaactgcct gtacgggtaa atccggggaa tcggcggttt
     4861 gtgcggtcaa tacggtttct tcggagctgc ccgcttcccg ccaggcatag ctgacttggc
     4921 ggatttcaaa tgatgcctgc accgactgtt ccgccctcaa ttctatattg gcatttttcg
     4981 gcggctgtcg gacaagtccg tcaaaatgtt ccaacagcgc ggcactgtct gcccgctcgg
     5041 ctttcacact gcaactgtcg cgcgtcaggc tcaaataata aatgccgcca tcgcgttcaa
     5101 gcgtccacaa cgcctgtaca tcgggttctg caacagtggg cggcagatgg gacaggtctt
     5161 cctttccgac agggacaaaa tgtccgacag cggcaaactt ggaaaccgat tcaaaactgc
     5221 cttgagtcag gacgcaggca ctccgaaaca aatcgacggc tacacgcgcg aaatcctgat
     5281 gccgtacacc ggattgccgg ttttgggcat tacacgcagc aagcacaaat gccgacagca
     5341 ataacatcaa tcgggacatc cgcatttcag acggcattta tttgtgttgg acgaccagcg
     5401 tgaacacgcc gtcctgttcg gaagcatcca acagcacatg accggtttgg cggcaaaaag
     5461 cctcaaaatc ccccggtgcg ccgccgtcgg ttgccagaac ggtcaacacg tcgccctgct
     5521 gcatttgcgc caaagccttt ttcgcccgca aaatcggcag gggacatttc aatccggtta
     5581 cgtctaaagt ttcgctgttc atcgtttgta ccgtttaaaa aatgggaatc ggggggagat
     5641 tataccccag ttgcttgcag ccccctttgc caacccataa aataacgccg ttttcaattt
     5701 gaagaaactt atgcgccgca ccgccctcct gctgctcgca ctcgccgccg gcacgtccct
     5761 cgctgccggc ttcagccaaa aagacacgcc gatcaacacc cgctaccgcg aaacgcccga
     5821 agaggccgcc gcccgcgaat ttaaagaaca taccgccaaa ctgccgccgc tgcccgatac
     5881 gcactcggac ggctggttcg acatttatgt ggatgaaaat tacggcaaac agccgaaaat
     5941 cctgctcgac agcctgcaaa ttatgcccgc gcccgacggc agcatccgct acattctcaa
     6001 tatccgctcc gacaaaggct acgacaacct gactgccgaa ggcatattct gcgcccgctt
     6061 ctccatcagg ttcggcaacg acaaactttc ctcctacaaa gtgttcggct acggcgacac
     6121 ggtcaaccgc cgctggatac agccccgaaa cgccgaatgg aaaccgatag gcggtacatt
     6181 aagccgcaac gacgcactgc gcaccgtcct gtatcaggca ttctgcgagg gcggtgtacc
     6241 cgccgacacg aaaggtttgg tacaacgcct gaaagaacgt gccagacgct atgccccgtc
     6301 gataaagccg cacgacaaat aaccagacac aaaaataccg tctgaaacat cggtttcaga
     6361 cggtattcga ttcatcagcc atcaggcggc agaaactttg tcctgacggc acaacagcgt
     6421 aatcaaatca ctgatgcgcc gcttcatatc gcggcggtcg acaatctggt cgattgcgcc
     6481 tttttccagc aggaactcgg cgcgttggaa gccttccggc agcgtttcgc gcaccgtctg
     6541 ctcaatcacg cgcggaccgg caaaaccgat cagcgcgttc ggttcggcaa gcacaacatc
     6601 acccaaaaat gcgaagctgg cggatacgcc gcccatagtc ggatcggtca acaccgatat
     6661 aaacggcagg cgtttttccg tcagcaaatg cagcgcggca ctggttttcg tcatctgcat
     6721 caatgagttc acaccctcct gcatacgcgc accgccggaa gccgccacac agacgaacgg
     6781 acaattgtcg gcaaccgccc ggcggacgcc ctgtacgaat cgttcgccca caaccgaacc
     6841 catcgaaccg ccgataaagc ggaactcaaa cgcagcaacc acaacgggca gaccgttcat
     6901 cttgcctttc attaccacca aagcatcatc ttccccggtc agcttgcgtg ccgcactcaa
     6961 acgatccgga tattttttgc cgtctttaaa cttcaaagga tctgtcggtt tgacatttcc
     7021 ggcaacctcc tctctgccat cctcatccaa aagcagattc aggcgttgtc gtgccgacaa
     7081 cggattgtgg tggttgcatt tcggacaaac ctgattgttc tgctgcaact cggtcgaata
     7141 aacggttgcc gaacaagacg gacatttgtg ccacaaaccc tcgggaacat tggaagaacc
     7201 gtctttgctg cggtttttga ttttgggtgg caggatttta tctaaccagc tcatggacaa
     7261 ctcctaaaat atttagaatg cgtgacagaa tcagcgcacg gcatccttca actctttgac
     7321 caaagcaccg acggccgcag cctcgttgcc cgcattgttt tcaatctctt tcacaatccg
     7381 gctgccgaca atgaccgcgt cggcaacccg gccgattttg cgcgcgcttt ccgcattgct
     7441 gatgccgaaa ccgacaccga tgggaatatc gatatactga tgcaaatact ctattttacg
     7501 cgaaacctca tccgtatcca aacttgccgc gcccgttacg cccttgagcg aaacataata
     7561 gacaaatccg cctgccagct cggcaatggt tttaatacgg tcttccgtcg tcgtcggcgc
     7621 aatcaggaaa atacagtcga ccccgttatc cttcagctcg cgatagagcg gatcgatggt
     7681 ttcgacaggg gaatccaccg tcaacacgcc gtccacaccc gccattgcgg cttcctgagc
     7741 aaactcccga taacccatct tatgtacagg gttcaaatat cccatcaaaa caaccggagt
     7801 ttgcgtgtcg gtttcacgga attttctgac gacatccaag acatcgcgca gcgaaatccc
     7861 gttcgccaac gcccgctccg ccgcacgctg aataaccggt ccatccgcca tcggatcgga
     7921 aaacggcacg cccaactcca aaatatccgc accgttttcc accatactgt gcattattgc
     7981 caaagtcgcc ccaatatccg ggtctccaac cgtaatatag ggaatcaatg cctttccgcc
     8041 atccaaagcg gcaaaagcct gccggattct gctcatttgt ctcttccatc ctatcggcaa
     8101 atatgccgta aaacacaatc agtaaatttt tcccagtata acatttgcgg tacggatagg
     8161 cgaatacggc aaacagaaac cgcgcaaatc agagccgccg tttgaacgag gcggaaataa
     8221 acggcaagca tctcgg
//
LOCUS       DAWKOD010000085         8208 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000085 DAWKOD010000000
VERSION     DAWKOD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.085"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(216..944)
                     /gene="rmpM"
                     /locus_tag="WJK73_001376"
     CDS             complement(216..944)
                     /gene="rmpM"
                     /locus_tag="WJK73_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ7557852.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1308..2258
                     /locus_tag="WJK73_001377"
     CDS             1308..2258
                     /locus_tag="WJK73_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ7557853.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2371..3105)
                     /gene="fnr"
                     /locus_tag="WJK73_001378"
     CDS             complement(2371..3105)
                     /gene="fnr"
                     /locus_tag="WJK73_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ7557854.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3310..4731
                     /gene="hemN"
                     /locus_tag="WJK73_001379"
     CDS             3310..4731
                     /gene="hemN"
                     /locus_tag="WJK73_001379"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ7557855.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5084..6658)
                     /locus_tag="WJK73_001380"
     CDS             complement(5084..6658)
                     /locus_tag="WJK73_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ7557856.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6983..8083
                     /locus_tag="WJK73_001381"
     CDS             6983..8083
                     /locus_tag="WJK73_001381"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ7557857.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLHSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2077 a         2298 c         2006 g         1827 t
ORIGIN      
        1 acggcaatgg gacacaaacc tgaatctgaa tctgaatctg aacctgcacc ccgattctga
       61 cgatagccgt ttcagaaaac cacccaaaaa aacaaacccc gcaaaagcgg gggtttgttt
      121 cacatcatcg atgccgaccg acgtttcacg aatattgcaa cagtacctgg tacaaaatcc
      181 actaacctca tgcatcggca agatattgcc tagccttagt gttggtgatg attgtgtgcc
      241 ggcacaacct gacgggttac gatgctgcgg attttcacat ccacacggcg gtcaggttcg
      301 atacatgcaa tcagagcctc acgttttttg gctttagaga ctttcgcacc cagtttggca
      361 acttcggctt cacaaacttg agtcatttgc gcttgagatt cgcccaagcc gacagcagaa
      421 attctagaaa caggtacgcc gttgctgacc aggttgtttg ccactacgta tgcgcggcgt
      481 tcggacaggg cctgattgta tttgtcagaa cccataaagt cggtatggcc ttcgacgcgg
      541 acagattgga tattggtttg acccaggcgt tgcgccaata ctttcaggtt gtcttgagct
      601 tcggcgcgca atgaatcctt atcgaaaccg aacagggttt tggcagacag ggaaatggtt
      661 tcatcaacat attgcggagc ctgctccaca acgacgacag gcgcgggtgc gggttcgggt
      721 tctggctcgg gttcgggggc agcaaccgca tcgccgcatt ctacgcgacc ttggcttgct
      781 ttatcaaagt aggcgttttt ccagcattcg ccatagttgt tgcgtacgat ttcgttcgac
      841 tggccgctta cggtgtaacc ctgaacggac gcctcgcccg caacagcagt gccggaagcg
      901 agcaatgcaa cgaataatgc gcttaatttc agctgtttgg tcattttatt ccctcattaa
      961 atttgtacag cagatacaga agcaactgct gaattgccaa catacaggcc gctgtagaaa
     1021 catggcggca aatcggatat ttcagtctcc actataaaga agcaggggga ggactgtcaa
     1081 tactcaacgc ctgaaaacaa ccgtaaaaca gcctgtttcc tgatagtcta tcatgccgaa
     1141 aaatcaattc cgtgtcatta ctttaggggg attttttcca tatcgcacga ttgccgttgc
     1201 acaaaaacaa caaacacaac ggcaaagccc cataccgtac cggcaagaaa atatgataaa
     1261 ttataaacaa tgttcagcca cccgacacag acccacaccg acccgccatg aaattacaac
     1321 aattgaaata cgccttagaa gtttaccagc acaacctgaa cgtttccgaa gcggccgaag
     1381 ccttgtttac ttcgcaaccc ggcatctcca aacaaatcaa attgctggaa gaagaaatcg
     1441 gcattcagat ttttatccgc agcggcaagc gcgtggtttc ggtctcgcag ccgggcaagg
     1501 tggttttgga tattgcggaa cgtattttac gcgatgtaca gaacattaaa aatatcggca
     1561 gcgagtttac cggacaggac agcggttcgc tgacggttgc cacgacgcat actcaggcac
     1621 gctatgccct acccttgatt gttgccgatt ttgtgaaacg ctatccgaaa gtcaacctga
     1681 ccatcaaaca ggggagccct gccgccatcg cacgtatggt tacctcaggc gaggcggact
     1741 tggcgattgt tacggaacgg atagacgacc atcccgaact gggaaaactt ccctgctatg
     1801 actggactca tgcggttatc gtaccgaacg accacccctt gctcgaatgc agaaaccccc
     1861 tccgcattga agatttggcg aggtttccgc tgattactta tgaatttgca ttcaatgcgg
     1921 gcagcagcat cgcgcgggca ttttccaaag cccgtttgga acagccggat gtcgcattgg
     1981 cggcggcgga tacggacgta ttgaagactt atgtgcgctt gggtttgggc gtgggactga
     2041 tggcgaaaat ggcgtacaac ccggatacgg acggcgattt gcagcttgtg gatgcggcac
     2101 acctgttcga gccgtcgccg acgtggattg ctttgcgcag cgatacttat ttgcgcggat
     2161 atgcctacga ctttatccaa gcgtttgcgc cgcacctgac acgcgagaag gtggatagga
     2221 ttctgtacac gcccatcagc gaggattttt cgatttaggc ggctgccggt tttcagacgg
     2281 cactttgcgg cagatacaac aaacaggaca gatgttttcg tctgccctgt gtttattgag
     2341 aatgctgtct gaaatgttcg tacgggttaa tcaaatggcg tgcgagcagc cggacaccat
     2401 ttttttcaac acctgcagat tgaggatttt gatgtgctta tgctcgacgg aaatcaatcc
     2461 ttcctgatgg aacttggata atgtgcggct gacggtttca agtttcagcc cgagataact
     2521 gccgatttct tcgcgggaca ttcttaagat gaagtcgttg gcagcaaaac ctcgggaata
     2581 aaggcgttgg gaaaggttca gcaggaaggc ggcaatccgc tcttcggcgc gcatattgcc
     2641 caacagcagc ataacacctt ggtcgcgcac gatttcacgg ctcatcatgc ggaagaagtg
     2701 cgtacgcagg ctggggatgt tttgccccag ttcttcgatg tgggtaaacg gcagttcgca
     2761 cacttcgctg tcttccaagg cgaccgcgtc gcaactgtgc acatgggaac agatgccgtc
     2821 catgccgatg agttcgcccg acataaagaa acccgttacc tgatcgcggc cgtcctgact
     2881 ggcgacggtt gttttgaaga agcccgaacg gatggcaaag agcgaggtaa aggcttcgcc
     2941 gacacagaac aggtattcgc cctttttcag gcggcggctt tgacggatga cggcatcgag
     3001 ttggctgagc tcgttgggca gcagcccgac aggcaggcag agttcccgca aagaacagga
     3061 agaacacagc gttttcatct gatgtgtagt attatgcgaa gccataccgt acctttttgt
     3121 gcgctttgcc ccatcatgat tatagtggat taaatttaaa ccagtacggc gttgcctcgc
     3181 cttgtcctta tttaaattta atccactata tgtgcttatt gacacatatc aagacaggtt
     3241 tatcatactg tggcattcta ccaaactatc caaaatttga ctaacatcat aaaaccgcac
     3301 acacacacca tgaaaatcat tcagatacag aacaatcaca atgtaaacga tgaccgcccc
     3361 gagtttgacc gcgcgctgat tgccagcctg cccgccagcg gcccgcgcta cacttcctac
     3421 cctaccgccg accgtttcca tgacggtttc cgcgaaggcg aatatatcaa agctttacat
     3481 ttgcgcggta tgggcgcgtt aaacaaaccg ctttcccttt acattcacat tccgttctgc
     3541 aacaccatct gctactactg cggctgcaac aaaatcatca ccaaagacaa aagccgcgcc
     3601 gatgcctaca tcgaatatct tgaaaaagaa atggaactgc tcgctccaca tctgaacgga
     3661 cggcaccagc ttgcccaact gcacttcggc ggcggcacgc cgaccttttt gagcgacgaa
     3721 cagatcgaac gtgtcttccg catgatacgc aaacatttcg agttaatccc caccggcgaa
     3781 tactccatcg aaatcgaccc gcgcaaagtc agccgcgaca ccgtcctcat gctcggcaga
     3841 ctcggcttca accgcatgag catcggcatt caggatttcg accccaaagt gcaggcggcg
     3901 gtcaaccgca tccaaagtta cgaagaaacc aaagaagtca tcgatgcggc gcgcgaagcg
     3961 gggttcaaat ccgtcagcgt cgatttgatt tacggcctgc cgcaccagac ttcggaaagc
     4021 atcaaaacca ccatcgatac cgttttgtcg ctcgatcccg accgcctcgc cctttatcac
     4081 tacgcccacc tgccgcacgt gttcaaaccg caacgccgca tcgataccgc ctccgtcccc
     4141 gacagcgaag agaagctcga tatgctgcaa tactgcgtcc aaaccctaac cgaacgcggc
     4201 tacgtcttca tcggcatgga tcatttcgcc aaacctgacg acgaactctc catcgccctc
     4261 aaagaaggct tcctccagcg caacttccaa ggctattcga cctacgcgga ttgcgatttg
     4321 gtcgccatcg gcgtgtcgtc catcggcaaa atcggcagca cctattccca aaacgaacgc
     4381 gacatcgatg cctactatgc cgccatcgac gaaggcagac tgcccatcat gcgcggttat
     4441 cagctcaatc aggacgacat cctgcgccgc aacatcattc aggatttgat gtgccgtttc
     4501 gcgctcgact atcggattta cgaaagtatg ttcggcatcc cgttcgaccg ctacttcaaa
     4561 gacgaactgg cggatttgga aaaactcgcc ggtttgggat tggtgcgcct gaacagccac
     4621 ggactgaccg ttaccccgaa aggacgcttc ctcatccgca acatcgccat ggttttcgat
     4681 taccacctgc gccataaaga aaccaaggcg aaatattcgc aaacggtgtg attgtggcta
     4741 acgtacaaat gccgtctgaa aggctttttc agacggcatt ttgctgccgg caggataagt
     4801 gttttcaaga acaggcggcg gcatatcata acgttccgca cctttgtgtc cgaccgttcc
     4861 gaaaccaaga tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgctgtact
     4921 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcac tataccaaac
     4981 ggcatatccc gacagaacag attgtgcata aggcacaagc ccgcacattc catcaacaaa
     5041 atgccgtctg aacacgggtt cagacggcat cagtatttta caatcagaat actgcctgta
     5101 aaaccaagta gcagacaacc gacaatacgg cggcggcagg cagggtaatg acccacgcca
     5161 aaccgatggg cttcatcagt ttccagttgg cattgcggtt gaccagaccg atgccgagta
     5221 ccgcgccgac caagatatgc gtactggaca cgggcagccc catcagcgac gcgcccatca
     5281 cgacggatgc ggcggacagt tcggcggtaa aacccgaagc aggatgcatt tccgccaaac
     5341 tcgtaccgac ggttttaatc acctctttac cgacaaacca caaaccgaca atcagcgcga
     5401 taccgaaagt cagcatcgca atcgggggga cgacattttg cgcggcaacg ctgttggtac
     5461 gcaaaacatc cataatcgcg gcaaacggac cgatggcgtt ggcgatatcg ttcgccccgt
     5521 ggctgaatgc gaagccgcag gcggtaaaga cctgcatcca tgaaaacatc tgaaaggtcg
     5581 atttgcccaa gtctttacgc ttgaggcttt tggcaaacac aaacgtcccc atccacaccg
     5641 ccgcgcctat cataaagatg gtcaggaagc tgttgacgtt gctcatcccc aaatgcaggt
     5701 ttttcaagcc cttgaaaatc agcatagcgg aaatcatcat cgcgccgaac gaagcgataa
     5761 agggaatcca agaatgcagt gccttgtagg aatcgacatt gtttttacgg ttgtcgaacg
     5821 cataaagacc gcggtaatac tccgattgca gctcttgcgg atcgaattcg ggttcgtcgt
     5881 aaatttgcgc gtcgtgcgcc attttggtgg cgtactcgac tttttcggct tcggacaaac
     5941 cctcgaaaaa caggcggtgc cgttctttat aggccttttt ttcctgcttg atgcccttga
     6001 gcgtgccttc cgcccaagcg ttgtaatcta agacgttttt cttgacgcgc gaaaacagga
     6061 aataggacac cgcgccgccc aacacgggcg acaataccca agaaacacca ataccgccca
     6121 gcttgcccca acgtatcaaa tcgcccgatg cggcatcgtt cattaccgcc atacataccg
     6181 cgctaccgac aatgccgccg ataatggaat gggtggtaga taccggaagc ccttttttcg
     6241 aggcaaacaa cagccacaac gccgccgcca aaagcgcgga catcataata aacacaaact
     6301 gtatgggttc gaaatcaaca cccttcaaat cgacgatgcc tttgcgtatg gtattggtta
     6361 cctcgccgcc cgcgatgacc gcgccgctga cctcaaatac cgccgcaatc agcaaagcct
     6421 gcgggatggt cagcgtaccc gcaccgacgc tggtgccgaa agaattggca acatcgttgc
     6481 cgccgatgtt gaacgccata aacacgccga acatcgtggc aataatgaat aaggcggcgt
     6541 aattgtaatg ggtataaccc aagccccaat aaataaaata gccgactgta cctaccagca
     6601 tggcggcaaa gacggcgttg atggttttaa catttgcgct catttggatt tgagccatgg
     6661 caatcttcct ttaatgtggt ggaaatcatg agtgttgccg acactgtact gctgcctccg
     6721 gtcgaactgc cgtttcggga tatggcagac gcatccttcc ttctgaaccg catccgaata
     6781 cccccaaacc tgaccgaatg caaaactgct gccggaaacg gtttgtatga cacttctact
     6841 actggatgtt gcgggcgcat tataatattt ttccatccgt cttgaaacat ttatttacac
     6901 tttatttaca ctgcggcggc aaatcggtat acgagcgtca atacacgtta aaatggcgtt
     6961 ttgcaccagt ttgggagtga tgatggaaac acagctttac atcggcatca tgtcgggaac
     7021 cagcatggac ggggcggatg ccgtactgat acggatggac ggcggcaaat ggctgggcgc
     7081 ggaagggcac gcctttaccc cctatcccga caggctgcgc cgccaattgc tggatttgca
     7141 ggacacaggc gcagacgaac tgcaccgcag caggattttg tcgcaagaac tcagccgcct
     7201 gtacgcgcaa accgccgccg aactgctgtg cagtcaaaac ctcgcgccgt ccgacattac
     7261 cgccctcggc tgccacgggc aaaccgtccg acacgcgccg gaacacggtt acagcataca
     7321 gcttgccgat ttgccgctgc tggcggaacg gactcagatt tttaccgtcg gcgacttccg
     7381 cagccgcgac cttgcggccg gcggacaagg tgcgccgctc gtccccgcct ttcacaaagc
     7441 cctgttccgc gatgacaaag aaacacgcgt ggtactgaac atcggcggga ttgccaacat
     7501 cagcgtactc ccctccgacg cacccgcctt cggcttcgac acagggccgg gcaatatgct
     7561 gatggacgcg tggatgcagg cacactggca gcttccttac gacaaaaacg gtgcaaaggc
     7621 ggcacaaggc aacatattgc cgcaactgct cgacaggctg ctcgcccacc cgtatttcgc
     7681 acaaccccac cctaaaagca cggggcgcga actgtttgcc ctaaattggc tcgaaaccta
     7741 ccttggcggc ggcgaaaacc gatacgacgt attgcggacg ctttcccgtt ttaccgcgca
     7801 aaccgttttc gacgccgtct cacacgcagc ggcagatgcc cgtcaaatgt acatttgcgg
     7861 cggcggcatc cgcaatcctg ttttaatggc ggatttggcg gaatatttcg gcacacgcgt
     7921 ttccctgcac agcaccgccg aactgaagct cgatccgcaa tgggtggaag cagccgcatt
     7981 tgcgtggttg gcggcgtgtt ggattaatcg catccccggt agtccgcaca aagcaaccgg
     8041 cgcatccaaa ccgtgtattc tgggcgcggg atattattat tgagcgcaaa taccgtctga
     8101 aagccatttc cgccgctcag acggcatttt cgcccctttc gtttacaaac ccttaaaatc
     8161 catttacact caaacccgtt caaaacaaaa caaaccctgc catgaaaa
//
LOCUS       DAWKOD010000086         8156 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000086 DAWKOD010000000
VERSION     DAWKOD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.086"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(84..1187)
                     /locus_tag="WJK73_001382"
     CDS             complement(84..1187)
                     /locus_tag="WJK73_001382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ7557858.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WJK73_001383"
     CDS             complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WJK73_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ7557859.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3116..3250
                     /gene="rpmH"
                     /locus_tag="WJK73_001384"
     CDS             3116..3250
                     /gene="rpmH"
                     /locus_tag="WJK73_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ7557860.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3253..3618
                     /locus_tag="WJK73_001385"
     CDS             3253..3618
                     /locus_tag="WJK73_001385"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ7557861.1"
                     /translation="MDYRFGKQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     VELAQLMFGNPATGCGKQV"
     gene            3683..3904
                     /gene="yidD"
                     /locus_tag="WJK73_001386"
     CDS             3683..3904
                     /gene="yidD"
                     /locus_tag="WJK73_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ7557862.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4076..5713
                     /gene="yidC"
                     /locus_tag="WJK73_001387"
     CDS             4076..5713
                     /gene="yidC"
                     /locus_tag="WJK73_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ7557863.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSAPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5794..6507)
                     /locus_tag="WJK73_001388"
     CDS             complement(5794..6507)
                     /locus_tag="WJK73_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ7557864.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCVAADLTLPTQEIISRTIAQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WJK73_001389"
     CDS             complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WJK73_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ7557865.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7304..7807
                     /locus_tag="WJK73_001390"
     CDS             7304..7807
                     /locus_tag="WJK73_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ7557866.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            7841..8020
                     /gene="rpmF"
                     /locus_tag="WJK73_001391"
     CDS             7841..8020
                     /gene="rpmF"
                     /locus_tag="WJK73_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ7557867.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         2003 a         2437 c         1949 g         1767 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
       61 gggcatcgtg ctccggaaaa ccgtcaaatt cgcatcggca tcacgatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggta tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa
     1201 atcggttgga ttttttaccc ttcggacggc atgggcaata tcatgccgtc tgaaaggcgc
     1261 gttcggtcag ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc
     1321 ttcctcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
     1381 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
     1441 cgccacctgg cgcggacggg caatgtttcg tgtccgtttc ttaccgagca catcgctgat
     1501 tttgatacgg taatatttgg caacggcatc aataatgata tcggcggtaa tgaccttatg
     1561 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
     1621 gttcataaag cggctgctcg ctccgacacg gttgaacgcg ccttcaagct cgcgcacgtt
     1681 ggaacggatc agattggcaa tgaacaacgc ggcttcgtct tcgatactga tgcccgccgc
     1741 ttccgccttt ttctgcaaaa tagcgatacg catttccaat tcgggcggct cgagttccaa
     1801 agtcagtccc catgaaaaac gggatttgag gcggtcgtcc ataccttcga ttttggcggg
     1861 taaaacatcg caagtgagga tcagctgttt tttctcgttg tggaaatggt tgtacagata
     1921 gaaaaactct tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag
     1981 caggtcgtat tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt
     2041 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
     2101 tttcaaaagc tcgttgccga ccgcctgtac caggtgggtt ttgcccaaac ccgtgctgcc
     2161 gtacaggaaa aacgggttgt agctctgccc cgggctttcc gcaatcgcct gcgccgcagc
     2221 cgccgcaagg cggttgccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag
     2281 attggtctgc tcgtaacgtg cctcttccgc atcgcgctgc gcatccgtcc gcgctttcgc
     2341 aagcacctcc gattccggac gggaagcagg ttcggcagta tggcgcggct catgcgggag
     2401 gtttttcata cgttccgcca gaatatccgc cgccgttttc gacgcagcgg gtttgacagg
     2461 ctcttcagac ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc
     2521 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
     2581 gccttctccc ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc
     2641 ttctattttt ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta
     2701 caccacccat acgcccccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg
     2761 cccgtgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
     2821 catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
     2881 aaaagaaaac aaatctgttg aacacacatc ggttcaaaat gcagcgaacc gaattatacc
     2941 gactcgccga tattttatcc acaacccgtg caaaatttat ccacagaaag gcggcagaaa
     3001 ttcgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
     3061 attggagtgt aattcacggt ttaattatct acccattcta tttttaggaa acatcatgaa
     3121 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     3181 caaaacacgc ggcggccgcg cagtattggc tgcacgccgc gccaaaggcc gcaaacgcct
     3241 ggcagtgtaa ttttggacta ccgcttcgga aagcagtacc gcttgttaaa aacggatgat
     3301 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
     3361 tcaaatggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     3421 aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt cagattgaac
     3481 aaaaaccggc tgccgccgca agatttcgtc gtgcgcgtcc gccgtaaatt cgacagggct
     3541 accgcaaaac aggcaagggt ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     3601 tgcgggaaac aggtatgatt agaacggtac tctgcaggca aggttcagac ggcaacgggt
     3661 ttcccataca aggaacatcc cgatgaactt tctattgtcc aaactcctgc tgggactgat
     3721 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     3781 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     3841 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgttcc
     3901 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     3961 aatgccgtct gaaacttcgg gctggcggca cgaccgtcgg gtatgaagcc cacccttatt
     4021 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
     4081 ttttaaaaga ctcacggcgt ttttcgccat cgcactggtg attatgatcg gatgggaaaa
     4141 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     4201 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     4261 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     4321 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     4381 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     4441 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     4501 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     4561 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     4621 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
     4681 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
     4741 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     4801 cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacccaaagg
     4861 cggacaaagc gtttgcgccg ctggcgaatg cagcatcgac atcaaacgcc gcaacgacaa
     4921 gctgtacagc gcaagcgtca gcgctcccct tgccgccatc ccggccggcg cgaaagccga
     4981 aaccgccgtc aacctgtatg ccggcccgca aaccacttcc gtcatcgcaa acatcgccga
     5041 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc ccctcttttg
     5101 gcttttgaac caactgcaca acatcatcgg aaactggggc tgggcaatcg tcgttttgac
     5161 catcatcgtc aaagccgtcc tctacccgct gaccaacgcg tcctaccgct cgatggcaaa
     5221 aatgcgcgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
     5281 ggcgcaacaa caagcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     5341 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     5401 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     5461 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cctatctgaa
     5521 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     5581 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     5641 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca cccaaggcga
     5701 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     5761 tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca caaaaatcgt
     5821 cgggcgtttt ttcagattgg gcatttcttt ccttttccgc cactgcgcga ttgtccggct
     5881 gatgatttcc tgtgtcggca aggtcaaatc cgcagctaca cacaagcgcg tttcagaatg
     5941 caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt cgataaacag
     6001 ctgcgtttca ttctgctggc gcgaacgttg ctccaaagcc ttcaaactct gaatccgctc
     6061 gtttttttca gacggcagat aacccttaaa cgcaaaactc tgcccgttcg cacccgaagc
     6121 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
     6181 atgcgccaaa gccaccagat tcgcacccgg atcggcaaca gccgggcaac ccgcctcgct
     6241 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccagca aagtcttcaa
     6301 atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa
     6361 atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatctg tcagcccgac
     6421 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     6481 aatcaaatac aaaacaggag acaccattcc ctcactcatc agttaaaaat gccgtctgag
     6541 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
     6601 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcgctgctt tcaatccgtt
     6661 caatcagcag cgcgcccaag ccctcgctct tcaacgcaca cgagcaataa accgcatcag
     6721 gctcgcgctc caaatagcgg aggatgtgca gctcgtccaa cttcctcatc acgaccaccg
     6781 tcttatcgat atgccgttgc attctgcccg taaccgtatt tagcagaacg accgcgctgt
     6841 aaaactcaat ctccctgccg ctcaaatgca tcaacatctt ttgcgcatta gcaaggttca
     6901 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     6961 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     7021 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     7081 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     7141 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
     7201 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     7261 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
     7321 gattgacccg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
     7381 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     7441 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
     7501 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     7561 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     7621 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     7681 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
     7741 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
     7801 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     7861 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
     7921 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     7981 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     8041 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     8101 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataataa cccgtg
//
LOCUS       DAWKOD010000087         8010 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000087 DAWKOD010000000
VERSION     DAWKOD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.087"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            281..1408
                     /locus_tag="WJK73_001392"
     CDS             281..1408
                     /locus_tag="WJK73_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7557868.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1438..2315
                     /locus_tag="WJK73_001393"
                     /pseudo
     CDS             1438..2315
                     /locus_tag="WJK73_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            2319..2507
                     /locus_tag="WJK73_001394"
     CDS             2319..2507
                     /locus_tag="WJK73_001394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ7557869.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(2584..4095)
                     /gene="lysS"
                     /locus_tag="WJK73_001395"
     CDS             complement(2584..4095)
                     /gene="lysS"
                     /locus_tag="WJK73_001395"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ7557870.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4092..4244)
                     /locus_tag="WJK73_001396"
     CDS             complement(4092..4244)
                     /locus_tag="WJK73_001396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557871.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(4250..5503)
                     /locus_tag="WJK73_001397"
     CDS             complement(4250..5503)
                     /locus_tag="WJK73_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7557872.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            5961..7757
                     /locus_tag="WJK73_001398"
     CDS             5961..7757
                     /locus_tag="WJK73_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ7557873.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1997 a         2242 c         2030 g         1741 t
ORIGIN      
        1 tagtgtatat taagtacgtc tgatatacac gataccctac gagggtgtaa gctttagttc
       61 acatttaaaa tgacctcttt aaacctgcct ttcggcaggt ttctttttag gttgtttgga
      121 aatcgtgtgc agacaaggtg taaaataggt aacaacataa aataatgcgg ttttaccgcc
      181 catatattta caaaagccaa atttttaaac atatatcctt gatatataca cggcgtaaac
      241 atatactgga aacatcttta aattttccga aattttaaat atgagcaact ggaaacccaa
      301 tattccctat aacgatttac cacccctgcc gccaaaacag gatattgaaa gcaaaaccat
      361 cctgaaacgt tgtatagccg cccgtgcatc ccttgcccgt ttaaagcagg cggcagaatt
      421 gataccgaat caagccatgc tgattaacac ccttcctgtt atggaagccc gtgcaagttc
      481 ggaaattgaa aacatcgtaa ccaccacgga caagctgttt caatccctgc aaatggatac
      541 ggaacggcaa gaccctgcca cgaaagaagc cctgcaatac cgcaccgccc tgtttgcagg
      601 ctatgaatca ctgacgagcc gccctttatg cacacaaacc gccatcatgg tctgcaacgc
      661 catcaagcac ccctacgaaa tggccatccg caaaacaggc ggcacagccc taaaaggagg
      721 caacagcgga aatgttgtct ataccccgcc cgaaggagaa gaaaccatac gcggcaagct
      781 ggcaaattgg gagcggttta ttcacgaaag cggcgattta gacccgctta tcatcatggc
      841 ggcggcacat taccaatttg aagccatcca tccgtttacg gacggcaacg ggcggacggg
      901 gcgcatattg aacagcctgc tattgattga aaaagggctt ttggatttgc ctattttgta
      961 tttgagccgc tatattatcg aaaacagggc ggactattac cgcctgcttt taggcgtaac
     1021 cgaacggcag gactgggaaa gctggataat ctacatctta gacggcgtag ccgacaccgc
     1081 cgattggacg gtagcgaaaa tagatgcgat acgccgcctg tttgagcaga cacgtcaaca
     1141 catacggaca cacgcacaag gaatctacac gcacgaactg gtaaatcttc tgtttgagca
     1201 gccatataca cgcattgcca acctagaagc ggcagggata gccaaacggc agacggcctc
     1261 taagtacctg aaagagcttt cagacatagg tgtgctgcaa gaaatcgcca tcggcaggga
     1321 caaactattc attcatccgc gcctaatgga actattgcgg ggagagggca acagctttac
     1381 ctcattccaa tccctcgtta aagcatagcc aaaataatca ataatccgga ggtcaatatg
     1441 gcaagaaggt caaaaacatt tgaagaagct gctgctgagg ttgaggaacg tttcggtcat
     1501 cgtggcatta agttggtcga gtttgagggt acagccaagc cgtgtgtaat caactgccct
     1561 aaacatggaa accaaacctg ttcgaggtac tccaatatgt tcataggaag tagctggggt
     1621 tgcccctctt gtggtaatga gcaagctgca aaagccggta tagcgaccct taggaagaat
     1681 cacatagcgt tagaaatgct gaaacaggct gtaacaggta tgaccaagca agagcgcatc
     1741 acgacgcaag cctacaatga gatgaccaaa tccgtggcag gttcaaacag catagtcctt
     1801 aacgatgtcc aaggcgatac gaccatcaac aaccatcata cgcatacgca caaccacagc
     1861 gatgccgacg gcaaagcact gtcgatgagg ctcacaccac gccccctgtt gtcagaccgt
     1921 caggcggcgg ctttcgcccg tacaggcaaa ctcacaggca gtttcgactt gtttgcttcg
     1981 gtggtcgccc cctcgcagta cacgtttgcc gttgccatgc ccgacacgtc catgtcgccg
     2041 gttatcgaaa aaggggattt ggtggtggtc gagccgcgta tgcgccctgc ggacgaggac
     2101 atcgtgctga ttgaactgtc cgacaagcgg ctggtcgtcg cgcaccttgt tatcgatatt
     2161 gcgggcagga tgctgattta tcagacgggc aggccgtctg aagcctttga cctgcccgaa
     2221 ggcagcacga ttttaggtgt ggtgctggag tcaaaaaacg gtttatgtcc gccgcacagg
     2281 caagaaggcg tgttgattcg gattaccacc cctgatgtgt ggacggttgg tacgatttcc
     2341 gcttccaaaa cgtcgtgtac gcgcccgacc gcagcccgga aatcagccgt atgctttctt
     2401 cgattttggc aggctacgcg tgggataccg aaaacccgtt cgtggcgaaa tccgaacaac
     2461 gcctgactgc cttgtccgaa tgggtcggtc agttagaaag cgaataaatc cgtaccgcca
     2521 tacaaaatgc cgtctgaatc caatcgggtt cagacggcat tgccatttca actgttttta
     2581 tgactactcg gggcgcatct gcggaaacag aatcacgtcg cggatggttt gcgaatcggt
     2641 cagcaacatg accaagcggt cgatgccgat gccgcagccg ccggtcggcg gcaggccgaa
     2701 ttccatcgcg cggatgtagt cggcatcgta gtgcatcgct tcgtcgtcgc ccgcgtcttt
     2761 ttgcgccact tgtgccttga agcgttcggc ttggtcttcc ggatcgttca actcggagta
     2821 gccgtttgcc agttcgcggc cgacaacgaa aagctcgaag cgttcggtca agcccggttt
     2881 gctgtcggat gcgcgtgcca acggggacac ttcgacaggg tagtcgataa tgaaagtcgg
     2941 gttccacaat ttgccctcgg cgcaaccttc aaacagggca agttgcaggc tgccgatgcc
     3001 cggagacgga ggcaggcttt cgccgtgttt gacgatttct tttttcagcc actccgcatc
     3061 gttcaactgc tcgtcggtgt agtgcggatt gtattttttg atggcatcga gaatggtcag
     3121 acgctcgaac gggctttcca aatcgacttc tttgccgttg taagtgatgt ttgccgtgcc
     3181 gtttaccgcg cgggaagcgt tgcggatgat gtcttccgcc atttgcatca tgcgttcgta
     3241 gtcggagaag gcttcgtaga attcgatcat ggtgaattca gggttatggc gcacagacat
     3301 accttcgttg cggaagctgc ggttgatttc aaacacgcgt tccaaaccgc cgacaaccaa
     3361 gcgtttcaga tacagctcgg gggcgatgcg caggtagagc ggaatgtcca aggcattgtg
     3421 gtgggtaacg aagggttttg ccgtcgcgcc gccgggaatc gggtgcatca tcggggtttc
     3481 gacttcgaga taatgctcgc ccaccataaa attacgcacg gattggatga tttggctgcg
     3541 tttgataaag gtattgcgcg attcttcgtt ggcaatcaaa tcgacatagc gttggcggta
     3601 tttggtttcc tgatcgctca agcctttatg tttgtccggc agagggcgca gggatttgga
     3661 cagcaggcgg atgccggaca cgcgtacggt cagttcgccc tggttggttt tgaacaaagt
     3721 gccttccgcg ccgacgatgt cgcccaaatc ccaatggttg aagtcgtcca aaacttcttg
     3781 gctcacgcct ttgttgttca gataaagctg gatttgcccg gacacgtctt gaatagtggc
     3841 aaagctcgcc ttgcccattt gacgcttcag catcatgcgg ccggccactt tgacgggaat
     3901 gccttgcgga tcgagttctt ctttgccgat ttcgccgtat tgggcgtgca aatcggcggc
     3961 gaagctgtcg cgtttgaagt cgttgggata ggcgttgcgc tgttggcgga tgttgtgcag
     4021 tttttcgcgg cgcagggcga tgatttggtt ttcgtccaac tgcggctcgg tttgcggatg
     4081 gttttgttcg ctcatagggt tttccgaaaa aataaatcag gcgcaatctg tttcagacga
     4141 cctgaccgaa tcacaaaatt tgcgcgtatt ttacgcgatg tcggcatttt tttccataaa
     4201 cgcggcaatg ccgtctgaaa gcggtttgcg gtttcagacg gcatcgttat catttgaaca
     4261 ttcccgccaa attcaataag aacaaaacgg taaaaccggt cagataaatc aagcctgcca
     4321 atgcaagggc attcatacct gatgtgagtt tgtgtttttc atcacctttg accaaacggt
     4381 aattcagcca ggcaaacaca ggagcggaca caaaagcggc aatcatcgca aatttgagca
     4441 gattcgccat tacgccgtca aaccaaaaaa tcaccgccaa accgctgccc gccacccaaa
     4501 tattccaggc aaagaattcg gcgttgcccg ttttgtcttt tccgcgcagc aggcgcacgg
     4561 gttcggcaat ggcacgggca tagccgtcca cgacggtaat cgtcgtgccg tacatacagg
     4621 caaacgcgat aaacgccacc agcgggcgcg accagccgcc gatggtaacg gcgtacatat
     4681 tgatcaattg cccgatatat ttgccgcccg ccatctgtac tgcttcgccg ttgccgtatt
     4741 gcacaaacgc acccagtgca aggaaaacca aagccaaaac cgcactggcg atataaccga
     4801 cgttgaaatc aaaaatcccg tcgcggtatt cggaaggatt gatgcgttgt ttttcggtta
     4861 cccacaaaga attgatggcg gaaatttcaa tcggcgcggg catccagccc atcagcgcga
     4921 tcaggaagcc caaaccggca agcgtccacg gtgtcggctc gataaaatcg gactgcatct
     4981 gcataccgcg cgacatagcg atgccggcgg cggcaagcgt ggcgatactc aaagtaacga
     5041 tgatgatttt ggaaacgcga tccaaagcgc ggtaacgtcc gctcaccaaa ataatcaggc
     5101 aggatgccat aatcaaggcg gcaaccgtgc cggcatcaaa catcagcgag ggaatcgcca
     5161 ttttgacgat ggcggcggtt acaatggcga ccgcgcccgc gttaatcgtg gcggagagga
     5221 tgcacaaaat caggaatacc cacaaataaa cgcggctttt ctcggcataa ccttcaatca
     5281 ggctcttgcc cgtgtccagc gtgtaatgcg cgctgaagcg gaaaaacggg tatttgaaga
     5341 ggttggtcag gatgatgatg agcgcgatct gccagccgta aagcgcgccc gcctgcgtcg
     5401 aggcaatcag gtgcgaaccg ccgaccgccg ccgaagccat cataatcccc ggtcccaatg
     5461 cgttgatttt acttttccaa gtcgaaatat gttgttcgga cataaagtct tccgtatttt
     5521 taaccgtgtt tcaacacaca gagccgcata ttcggacaca gccctatcta ttgctccaat
     5581 ttgggcggga ttgcccccaa acaaacccaa atcctaccgt cttcaaaaac aggataccgc
     5641 ccggtaggga aattttgatg aaagcgcgta ttgtaacgta atccaaatac ctgccaacac
     5701 acactattag aacttcatgc tcaaacttga ctatattttc cgtattactt ccaaaaaaat
     5761 gcataaaacg atatcttatg cctaaaattt tacaacaaac aaccttacat cgcttttttc
     5821 gcgcaaacac gcaccatccg atcagcccgt ccgttttgca gcaggctggc gatttgataa
     5881 gatggttatg tttttcagac ggcatttcag atttccgtcc atgccgtctg aagccgcaaa
     5941 acccgattgg aggaactgtt atgaataccg tatcgaatta cctgtccgca ttgcgcgaag
     6001 ccatgaaggc gcaaggcttg gatgcactcg tcatcccttc cgccgacccc cacctgtccg
     6061 aatacctgcc tgagcattgg caggcgcgcc gcgaattatc gggctttacc ggctcggtcg
     6121 gcacgtttgt cctgaccacc gatgaagcgg gcgtatgggt ggacagccgc tattgggaac
     6181 aagccgccaa gcagcttgcg ggcagcggca tcgaactgca aaaaagcggg caagtgccgc
     6241 cgtataacga atggctcgcg gcaaacctgc ccgaaaacgc cgccgtcggc atcccttccg
     6301 atatggtctc gctcaccggc aaacgcactt tggcgcaatc cctgaccgcc aaaaacatcc
     6361 gcatcgaaca cccggacgat ctgcttgacc aagtgtggac aagccgtccc gctatccccg
     6421 ccgaaaccgt gttcgtccac gaccccgcct acgtctccga aaccgccgcc gaaaaactcg
     6481 cccgcgtgcg cgccgtgatg gcggaaaaag gcgcggatta ccacttggtt tcctcgctcg
     6541 acgacatcgc ctggctgacc aacctgcgcg gcagcgacgt gcctttcaat cccgttttcg
     6601 tgtcctatct attgattggc aaagacaacg ccgtcctgtt taccgaccga tgccgtctga
     6661 atgccgaagc cgccgccgcg ctgcaaaccg caggcatcac ggtcgagcct tacgcccaag
     6721 ttgccgacaa actcgcgcaa atcggcggcg cgttgcttat cgaaccgaac aaaaccgccg
     6781 tcagcacgct tgtgcgcctg cccgaaagcg tgcgccttat cgagggaatc aacccgtcca
     6841 cgctgttcaa atcctgcaaa tccgaagccg acatcgcccg catccgcgaa gcgatggaac
     6901 acgacggcgc ggcgttgtgc ggtttcttcg ccgagtttga agacatcatc ggcaacggcg
     6961 gcagcctgac cgaaatcgac gtggacacca tgctttatcg ccaccgcagc gtgcgcccag
     7021 gcttcatttc attgagtttc gacaccatcg caggcttcaa cgccaacggc gcactgccgc
     7081 attacagcgc aacgcccgaa agccacagca ccatcagcgg caacgggctt ttgctcatcg
     7141 actccggcgc gcaatacaaa ggcggcacga ccgacatcac ccgcgtcgtc cctgtcggca
     7201 cacccaccgc cgaacaaaaa cgcgacaaca ccctcgttct caaagcccat atcgcgcttg
     7261 ccgaagccgt gttccccgaa aacatcccct cgccgctgat tgatgcgatt tgccgcaaac
     7321 ccctgtggca ggcgcaatgc gactacggcc acggcaccgg acacggcgta ggctatttcc
     7381 tcaacgtcca cgaaggcccg cagcgcatcg ccttcgccgc ccccgccacg cccgaaaccg
     7441 ccatgaaaaa aggcatggtt acctccatcg aacccggact ctaccgcccg ggaaaatggg
     7501 gcatccgcat tgaaaacctt gccgccaacc aagccgtcgc cgcccctcaa gaaaccgaat
     7561 tcggcagctt cctctgtttt gaaaccctga ccctctgccc catcgacacc cgcctgatgg
     7621 acaccgccct catgaccgac ggcgaaatcg actgggtcaa ccgctaccac gccgaagtcc
     7681 gccgccgcct cgagccgctg accgaaggcg cggcaaaagc gtggctgatc aaacgcaccg
     7741 aaccgctggc gcgttaaaca gcacggcgca aaaaatgccg tctgaaagcc cttcagacgg
     7801 cattggtttc ccaaaacatc ccgcaccgtt ttcatcttgc cgcaagcaaa tatagtggat
     7861 taacaaaaat caggacaagg cgacgaagcc gcagacagta cgaatagtac ggaaccgatt
     7921 cactcggtgc ttcagcacct gagagaatcg ttctctttga gctaaggcgc gacaacgccg
     7981 tactggtttt tgttaatccg ctatattccg
//
LOCUS       DAWKOD010000088         7968 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000088 DAWKOD010000000
VERSION     DAWKOD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.088"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..140)
                     /locus_tag="WJK73_001399"
     CDS             complement(<1..140)
                     /locus_tag="WJK73_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7557874.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WJK73_001400"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WJK73_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ7557875.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WJK73_001401"
     CDS             1619..2668
                     /locus_tag="WJK73_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7557876.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WJK73_001402"
     CDS             3356..4777
                     /locus_tag="WJK73_001402"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ7557877.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4948..5727
                     /gene="xth"
                     /locus_tag="WJK73_001403"
     CDS             4948..5727
                     /gene="xth"
                     /locus_tag="WJK73_001403"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7557878.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKALSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5714..6058
                     /locus_tag="WJK73_001404"
     CDS             5714..6058
                     /locus_tag="WJK73_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557879.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            6069..6599
                     /locus_tag="WJK73_001405"
     CDS             6069..6599
                     /locus_tag="WJK73_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7557880.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6827..7942)
                     /gene="asd"
                     /locus_tag="WJK73_001406"
     CDS             complement(6827..7942)
                     /gene="asd"
                     /locus_tag="WJK73_001406"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ7557881.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2076 a         1987 c         2077 g         1828 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcgggtt tggggcatcg tttcctcaaa
     1081 cacttatcgc gcaccggatt attgctgcac gtcgtcgatt tggccccgtt tgacgaaacc
     1141 gtcaaccccg ccgaagaagc cctcgccatc atcaacgaat tgcgcaaata cgacgaagaa
     1201 ctctatggca aaccgcgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccaaacgc gcaccgtcac cttcctcgaa gccgtcggct gggattaccc gaaaccggac
     1321 gaccgcttcc aattcgatat ggaaaccccg cgcctcttcc aaatcagcgc gctaacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgcttg
     1441 gaagccgaaa aagcagaagc ggagaaagcg gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatgcctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctaa cccgtcaaaa agaacccttt tcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtctgcg gtatgaccgt ttacgattac tgccatttgg gacacgcccg
     3481 cgtgatggtg gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcaaactga ctgcgcgttt catccaagcc atgcacgaag atgccgatgc
     3661 tttgggcgtg ttgcgtccgg atattgagcc gaaggcaacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaattg tcgggcaaat cgttggacga
     3841 cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gccgccaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gccgcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggccgt ctgaaagcct tgggcggcat
     4561 catcggcctg ctgcaacgcg acccgaccga gttcctgcaa ggcagcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat cgcccgccgc aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttgaact gtatcggaaa gggcaaaagt
     4801 accgcctttt gctctttttc cgttttcaga cggcatttcc cgcttgaagc cccgccgttt
     4861 tcagcaggca gggccgaatg ggaaatgccg ggcggcgcac cgggtgctgc tttgtgtatg
     4921 ggaacattgt gagaacttat ttttattatg cttaaaatca tttccgccaa cgtcaacggc
     4981 atccgctccg cctacaaaaa aggtttttac gaatacatcg ccgcatcggg cgcggacatt
     5041 gtctgcgtgc aggaactcaa agcgcaggag gctgatttgt ctgccgatat gaaaaatccg
     5101 cacgggatgc acggccattg gcattgcgcc gagaagcgcg gttacagcgg cgtggcggtg
     5161 tacagcaaac gcaaacccga caatgtgcaa atcggtatgg gcattgaaga attcgacagg
     5221 gaagggcggt ttgtgcgttg cgatttcggc aggttgagcg ttatttcgct ttatttgccc
     5281 agcggcagca gcgcggaaga acgccagcag gtgaaatacc gtttccttga tgcgttttac
     5341 cctatgctcg aagcgatgaa aaacgaagga cgcgacattg tcgtttgcgg cgactggaac
     5401 atcgcccacc aaaacatcga cctgaaaaac tggaaaggca atcagaaaaa ttcgggcttc
     5461 ctaccggaag aacgcgagtg gataggcaag gttatccaca agctcggttg gacggatatg
     5521 tggcgcacgc tttatcccga tgtgccgggc tacacgtggt ggagcaaccg cgggcaggcg
     5581 tatgcgaaag atgtcgggtg gcgcatcgat tatcagatgg ttacgcccga acttgccgcc
     5641 aaagccttgt ccgcacacgt ttataaagat gaaaaatttt ccgaccatgc gccgctggtc
     5701 gtggagtatg actatgctgc cgaataaagt tttgggtaaa tatgattgga atgtggacgg
     5761 aaaaacagga ataggcgcgg catgggttgt cgctgcgttt atcctgccca tgttggtgtg
     5821 ggcgatattt atgttgtcgc ggatgcaagg ctggcttgcg ccgaccaagg caaacccgat
     5881 atgggcgttg gtgtggctgc tgatttgcct gccctgcctg ctgattgcgt ccaaatgttt
     5941 gggttggaaa ggctggcggc gggtggtgaa tatttttgcc tgcctgaccg tctgcgccat
     6001 attgagcgta cccgcttcgc tgctcatcgc ctttaccctc cgggacctgc tgaaataggc
     6061 ggggcggtat gcagggtttt gaactcgaag atttgacgct ttggctgata cgcgatgccg
     6121 gagaggatca gatgtggata gaccgttggg cgatcagtta tcccgtcgtg cagatgtccg
     6181 aagcgtcggc cggtcaaagc ataggggaat ggcaggcagg gcttcagacg gcatttgaac
     6241 gcatacgtgg caaatacgtc gccgttgtcg cacacggtgc gggcgcggcc gcatttttgg
     6301 cgtggctgta tcaggcagac atcctgacac ggaagaaaat tgccaacatc atccttgtac
     6361 cgcagcgtcc cgatattttt cccgacgatg cggaacacac tttccaacgc gtccgctgtc
     6421 cctgccgtgc cgcattggtt gtacccgaac acggcggcgt gccgcacggt tgggcgcaaa
     6481 aacaggcgga tttgtggaac gcccgcctgt tggtttcccc gcattcgggc agtttgaacg
     6541 gcatgctcgg cggctggcag tggggtatga agctgatgca ggaaatgttg ctggcgtgaa
     6601 cgcttgcctt atcggaattt cggctgtagc aggaagtccg caatcgggct gttttcattg
     6661 acgtgcaggc gcatcaggtt cggataaagt cgggctgccg cataccgccg gaaatggaat
     6721 cctttatttt ttacgtcggc agtgccggca gggcgttatc cggcagggtt gcaaggaaaa
     6781 tgccgtctga aacaggcttc agacggcatt tccattcaaa cagggcttac aggctgccca
     6841 acacgatacg caatacgcgg cgcagcggct cggccgcgcc ccacaagagc tggtcgccga
     6901 cggtgaacgc gctgatgtat tcgccgccca tgcccagttt gcggatgcgt ccgacaggga
     6961 cggacagcgt gccggtaact ttggcaggag tcagctcgtg gatgctggct tctttttcat
     7021 tggggatgac tttcacccag tcattcgcgc ctgccaaaat cgtttcgatt tcggaaacag
     7081 gcaggtcttt tttcaacttc agagtgatgg cttggctgtg gcagcgcatc gcgccgacgc
     7141 ggacgcacag gccgtcaatc acggttggat tgtcgctgcg gccgaggatt ttgttggttt
     7201 ccacgccgcc tttccattct tctttggact ggccgttgcc caaatccacg tcaatccacg
     7261 gaatcaggct gccggcgagc ggtacgccga agttggcttt cggatagtct tcgctgcgca
     7321 ggaaatcgga tactttgcgg tcgatgtcga gaatcgagcc ggcaggatcg gcaagttcgt
     7381 ccgccacttg ggcgtgaacc gcgcccatac cgctgatgag ttcgcgcatg tttttcgcac
     7441 ctgcgccgga agcggcttgg taggtcatgc tggttgccca ttcgaccaaa tcgttttgga
     7501 acaggccgcc caaagccatc agcatcaggg agacggtgca gttgccgccg atgtagtttt
     7561 tcacgccgtt tttgaggccg ttgtcgataa cgttgcggtt gacagggtcg aggacgataa
     7621 tcgcgtcgtc tttcatgcgc agagaagatg ccgcgtcaac ccagtagccg ttccagccgc
     7681 tgtcgcgcag ggcttggaag acggatttgg tgtaatcgcc gccttggcag gtaacgatga
     7741 tgtccatttt ggcgagttcg gcaacattgt tggcatctaa taatgtttta gccgcctgac
     7801 cgaaatcagg ggctgcgccg ccgacgttgg aagtggtaaa gaaaaacgct tcaggaatgt
     7861 gggcgaagtc gttttcttct ttcatacgct gcatcaaaac cgaaccgacc ataccgcgcc
     7921 agccgacgaa acctactttc attgtgttgc tccttgagga aaaatcgt
//
LOCUS       DAWKOD010000089         7873 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000089 DAWKOD010000000
VERSION     DAWKOD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.089"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1082)
                     /locus_tag="WJK73_001407"
     CDS             complement(120..1082)
                     /locus_tag="WJK73_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ7557882.1"
                     /translation="MRNAVGLDISKLTFDAFAMVGNAEYSAKFDNNSKGLNQFSDWLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAEYCRSAQESELVKRQKPTDEQYRLSRMTVAYTQIKSECAAMKNRYQVAKDEEAAK
                     AYAEIIKAMNEQLEVLKAKIKEQTEKPKCKEGVKCLETIPGIGRMTAAVLFHHLTSSQ
                     FETSNKFAAFAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMVAYRIRAFPDF
                     IARLEAKKKPKKVIIAALMRKLAVIAYHVHKKGEHYDPSRYKSA"
     gene            complement(1596..1955)
                     /locus_tag="WJK73_001408"
     CDS             complement(1596..1955)
                     /locus_tag="WJK73_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557883.1"
                     /translation="MINKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RVARKDSLFEVDYIYGADGCGFLPSNLTEFKKYCRKQYQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(2076..3161)
                     /locus_tag="WJK73_001409"
     CDS             complement(2076..3161)
                     /locus_tag="WJK73_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ7557884.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYSWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIIGFVGWKSFGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(3171..3461)
                     /locus_tag="WJK73_001410"
     CDS             complement(3171..3461)
                     /locus_tag="WJK73_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7557885.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(3462..4967)
                     /locus_tag="WJK73_001411"
     CDS             complement(3462..4967)
                     /locus_tag="WJK73_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7557886.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDKRRINRTYGCYGVDSSIMRLMSDDSR
                     FPEVKELMQSQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGD
                     DYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPG
                     TKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEV
                     SPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHR
                     KERKEGEDGGLLCDYFPEILACQEMGKPSDGMFHDISIPQVIDDKTWSSHNFLPSNGV
                     CPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLLAFIIMSAFVVFGSLKGK"
     gene            complement(5426..5704)
                     /locus_tag="WJK73_001412"
     CDS             complement(5426..5704)
                     /locus_tag="WJK73_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557887.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(5711..5929)
                     /locus_tag="WJK73_001413"
     CDS             complement(5711..5929)
                     /locus_tag="WJK73_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557888.1"
                     /translation="MNIMNTCRKYGAKLAVLTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(6015..6218)
                     /locus_tag="WJK73_001414"
     CDS             complement(6015..6218)
                     /locus_tag="WJK73_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557889.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDPFGDIGFTPNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
     gene            complement(6232..6543)
                     /locus_tag="WJK73_001415"
     CDS             complement(6232..6543)
                     /locus_tag="WJK73_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557890.1"
                     /translation="MFETSQVTTYPATLLGAKQFKGEIDGNKIDSCTVLVASPMSSNG
                     NAVGFTAASMKFGDSHNFEKLKNLKFPCAVDVTVAMESTGKGLVPKLLDFQVKGAAPK
                     A"
     gene            complement(6557..7858)
                     /locus_tag="WJK73_001416"
     CDS             complement(6557..7858)
                     /locus_tag="WJK73_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7557891.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     QYSELLEEILGFGITRKLPFKGKFFYQSCYQLGPDNVEYGKVHYGGQRETMLVELNGT
                     GCMAALPGWENRLHEFLSKCVRPKITRVDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQFGDVDSPWVRFETEFRAG
                     DIEIPLDILLYSGSYLGGAYPICKAIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGK
                     MVNFLRDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALN
                     IETLFDDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
BASE COUNT         1945 a         1853 c         1603 g         2472 t
ORIGIN      
        1 ttgacaatag catctttaca attcattttc aactcattga atttcaatat ttaaaaattc
       61 actggatttc agacacggca aagcaggcat ttaaaaaatg ccttttttcc tttcaggatt
      121 tacgccgatt tgtaacggga tggatcgtaa tgctcgcctt tcttgtgcac gtgatacgca
      181 atgacggcaa gtttacgcat caatgcggcg atgatgactt ttttaggctt cttcttcgct
      241 tccagccttg caataaaatc aggaaatgcc cttatgcggt atgcaaccat ggcaggcata
      301 aacaagacgg cgcgtaattt cctgttgcca aacttggtca gtttaccttt tcctcttacg
      361 cttgtgcctg attctttttg ttgcgggctt aggcctgcga acgctgcaaa tttgtttgat
      421 gtttcaaact gcgaagatgt gagatgatga aacaatacgg ctgcggtcat tcttccaatt
      481 cccggtatgg tttcaagaca tttcacgcct tccttgcatt tcggcttctc cgtctgctct
      541 tttatcttcg cctttaaaac ttcaagctgt tcattcatgg ctttgatgat ttctgcatat
      601 gctttagccg cttcttcatc ttttgccacc tgataacgat ttttcattgc cgcgcattcg
      661 cttttgattt gcgtatatgc taccgtcatc cgtgaaagcc tgtattgctc gtctgtcggc
      721 ttttgccttt ttacaagctc gctttcctgc gccgaccggc aatactccgc tatcagtttt
      781 gcatcctgtt tgtctgtttt ggtgcgtttg aatctgcttt ctgcatactt gcttattttc
      841 agcgggttca ctacgtaaac gctgtaatac tgcgcgaagt agtcggcaac ttcttcataa
      901 tagtttcccg ttgcctccat acagatatgc agattctgac atcccaagct tttcaaccag
      961 tccgaaaact gatttaaacc ttttgaattg ttgtcaaact ttgccgaata ttcggcattg
     1021 ccgaccatgg cgaatgcgtc aaatgtcagc tttgatatat ccaatcctac ggcgttacgc
     1081 atgggattac ccttatctat tcagaatcta tgttctttga tactactcaa tttcacaaac
     1141 aagaaaaccg cccgcctatt ctcgtcatca aactttaagt ttgtggttta ttcagactgg
     1201 acggttgcgg caaagggtag ctattccttt gccgtgtctg attttatttg ggttgcaggt
     1261 tttggtaaag gtttctgttg cgacccgaat gtctgatttt ttttgggcgt atctcattct
     1321 agaatcactc cgttagtggg tttccggtat tgaaaaacag ttcataaaaa aggaaaaggg
     1381 ggtattcata aagattgggt aaaaagcgcg cccaatcttt acaaagcttc ccccttttcc
     1441 ttttttctgc cctattttcc tgcacctaca acccccgaac gaagcgattc tagactgaga
     1501 cactttaaaa aaaacagcca ttctagcagt taaccccctt cactcagctc aagccatcct
     1561 gaggtatagt ggctgaattt gtgattttgg ttttatcaaa caaaatattt gactgaagtc
     1621 acatggcggt cgtcatatgg gggttccttt gcacccaaaa aatcgaccgc gtaacggttt
     1681 actaaaactt catcgtcctt aaacttttgg tactgtttcc ggcaatattt tttgaactcc
     1741 gttaaatttg atggcaagaa tccgcaaccg tctgcaccgt aaatgtaatc aacttcaaac
     1801 aaactgtctt tccgggcaac ccgaccatct ttaagccaga acataggctc gaaataaaaa
     1861 actttcgacg tgtccggaaa cttatgaaga cggagaacac gtgtgaaatc cccgttttca
     1921 tctacaattt tgatatatct tggtttgtta atcatgacat cctcagattt agtattcaga
     1981 atatgatttt aaaaagaact ttctgcttta cgactccgcc gccaattcct tcaaacggtt
     2041 ttccgcgctc ttcagttgtc gtacatgaga ttttgctacg gctttccgcc cattacgaga
     2101 acttgcggct tgtccgcttt cgcggactgt tccacctgtt ccgtcctttg ctgttcgtcc
     2161 ttataaggat tgaaaggcaa cccgtttttc acatattctt tacacattat ctttgttatt
     2221 tctttcaagg gtgttccttg atttgaatag catgtgcaat ctgattttcc gccgtctata
     2281 catccggcga tttgctcaaa ggtttttact tgtcggactg tgttataaat aggcttgctt
     2341 tcgggctttt cgggtaaagt cggcacaaag tcttcaggtt tcaaattgtc tgaatttttt
     2401 aaaggcattt cctctgatga tgcaggctgc tccgtcatcg tctgcacaac gctttctttt
     2461 tgcgcttcct gctcaatccg gctgtctgtg gctttgctgt aaactttaaa aatgccgaaa
     2521 cttttccagc ccacaaaccc tataatcgca attaacgccc aaaccgccca aggaactttt
     2581 ttcttgaact tttggtgctg gcttgctgat ttatagtatt tgaaggcttc tttcggcggt
     2641 ttccaacttg cgacttctac gccgcttacg cctgcgggat tgtccaacga cgttacacac
     2701 ttgtaccaag aatactgctt catgccgatt gccttgcgtt caaggtgtac atgctttgaa
     2761 acaaggttgc ggacgaatat atcaagttgg ctcgggtgct gcgtcatcaa aataacggta
     2821 tgcccgtgat ggcggagttc tgtcagttcc tgaatataag gcggaacggg acggcctgcc
     2881 gcgcgtaccg gataagtgta gtgcgcttcg tcaacaatca gcactgcgcc ttccggtatg
     2941 acatcacgaa gcggggcgga catgatttgc tcttccgtca gttcgtgggc tttaaactgc
     3001 cgtttatcca atccgtcgat atggcagaaa taaagcggcc tgtctacctc tgtgccgtct
     3061 tccaatttca ttttgaacaa tccgtcttcg ttgttcaaaa tcatagagac gacgcgggag
     3121 gttttgcctg tccccatgtt tcctgtaaac agataaatca tgcttctacc tcatcctgga
     3181 aagacaaacg tcagtttttt gaatgcgtgc ataccaatga agaacgagaa tgcgccgaac
     3241 aggtagccca aaccctgccc gaatcccgaa attaaaagaa ggttcagtat gtcggaaggc
     3301 atggaattaa tcgcatttga cgtgtagtct ttgaactttt ccagcgcgat gagataaccg
     3361 gcataggtta caaatgtcag acctgttgca aggatgattc tgacaatcag cattttcaga
     3421 agtatgccta aaagtggaat cagaccggca agtaatggca tttatttccc cttcaacgaa
     3481 ccgaaaacga caaaagccga cataatgata aaggcgagca gtacggcaaa acggattttt
     3541 tcggcaaaca cgcacaacgg ctcatagctt gcctgatatt gcctaccgaa aacatgaaag
     3601 gttttcggct gcggacatac gccgttagat ggtaaaaagt tatgtgaaga ccatgtttta
     3661 tcgtctataa cctgcggtat gcttatatcg tgaaacatgc cgtctgaagg tttgcccatc
     3721 tcctgacagg ctaggatttc cggaaaataa tcgcacaaaa gcccgccgtc ttcgccttct
     3781 ttcctttctt tgcgatgcct accgtttggg cggtccggaa cggcggggga atcgggtctt
     3841 gtgccgggct gtccgtccgt atcgggattt gcatcgggat tcaaatcggg gtcgggttcg
     3901 ggattggggc tcgtgccggg gttctcattg gggttcgggt tgtttgcggg gttttcggcg
     3961 ggcgatactt cgggcagcgg ctgtgcgttc ggtgcttccg cgcttccggg tgtgaggtcg
     4021 ggacgcggga ttacttgaac atccaccgtg gtgttgcctt gcgaatccct gccgaatgtt
     4081 gcgacaacct gaactggatt cccgttcctg tccgtgacgg gacccatatt cacttttgtt
     4141 ccgggtgcga cttctacttt ttcggaataa ccgggatatc cggttgcctt tatgtatttg
     4201 tcgggattgg catcgacttt caacgataaa atctcttcca gctttttggc atccatttct
     4261 tctttgtatt tcggattgcg gctaagggaa aaattagccc catttctgta atcatcacct
     4321 ttattgacca cacaatctcc gccgttccaa tcaaatgtgc aacgatttaa aacaaaatta
     4381 ttccaatcca aagaacttaa tttattcagt tcttctttat gccaattcca aaacggacgt
     4441 gccagcctat acatttggct ttgcatcaat tctttgactt cggggaatct gctgtcatcg
     4501 gacataaggc gcataatcga actgtcaacg ccgtagcagc cataggttct attaatacgt
     4561 cttttgtctt cgtaccaaag gcaattacta tattcgtagc cttttacaaa tttgtcggtt
     4621 tcggggtcgt attggtagcc ttgtgcctgt atgtcttctt tgaaagtttc gtatacgtca
     4681 tgggctaaaa gggctgttcc gacataagga actgcccttg tgcttaattt cgcgcctaag
     4741 cgggcaagtt tgccgactcc tgacaagacg gcggcgcggg aaactgatgc agttacttta
     4801 acggggactt tttcaagaga gcgtgcgcct gttgaacttt ctaatacatt caaattcaaa
     4861 cttttatcaa atttgtaatt atattctgta tgaattcctc ctccctcacc taaaactttg
     4921 tatgccttaa atccattatc gttatatttt tccgaaagtg catacatcaa tttcccattt
     4981 ttaatttcta aatctgctga aaaagattta gcactcaaaa gaaacagaaa agaaattatt
     5041 agaatccgaa acatcaattt ttccattgcc aataatgaaa aatgaatcat ccttgaattt
     5101 aatttcaaag caagattcat taaaattaat tctattaaaa aaattatgac atttatccat
     5161 tgagaaattt ttcaatagac cagtttcttt caaataacaa taaaacacat aagaaagagg
     5221 cctatcagga taagattctg acaatttatc taaatcagaa tttgatatat agaaaaagtc
     5281 acgttcattt ttattcatgg tttcaagcct caaatgtcta attaaaagcc tgattttgac
     5341 accataactt catgcgctca atccttaaac agaaccgccc caattaatac gggtacggaa
     5401 acgccgagat aaaaataaaa atccatcatt tcaaaacctt tttcagcagg gaaacaaagt
     5461 aaacggacgc gaggatgccg aatactatcc agcctgtttc aagaccgctt tgcaggttgt
     5521 ctttcggact gcattccgcc aataaaagcc ttagcggctg accgtccgac atcttccaca
     5581 ggctgccgtt atattccggc ctgacaatct gtccgttttc tttgattctt ggtactacca
     5641 agctgaaata aaggttttca gcctggtgct tctcaagaca tttatttccg acttggtagt
     5701 acatgccgtc ttacttcatc actctcttaa cgatggaaaa gacaaaaagc gcggcgaaaa
     5761 cgccaacgac aatccaaccg gcttccatac cgtcggcttt tgcggcttcc aaagcgtttt
     5821 ttgccgtatc gggcaacgtt gcatttgcat gtgcggccaa agccagggga gcagctgtta
     5881 gaacagccag ttttgcgccg tatttacggc aggtgttcat gatgttcatg atattttcct
     5941 ttacgaaatt tttaaaaaaa tgtgtttgcg ggctttgtga aggttttaga gaccgcccgc
     6001 cgagcctctt aaacttaatc ttctttttcg tagaatccga aaattacaaa ttccccgcct
     6061 atctcttcca atgccgagct aaaagcgtct tcatagctgt catatttacc ggctgatttg
     6121 atgttcggcg taaagccaat atcgccaaac ggatcgggat agatgaattc gtgattttcc
     6181 aattcctgta cgataaattt ctgttggtat ctgctcatga ttcagccttt cttaggcttt
     6241 aggtgctgcg ccctttactt ggaaatccaa caatttcggc actaagcctt ttcctgttga
     6301 ttccattgca acggttacat caaccgcaca ggggaattta aggtttttca atttttcaaa
     6361 attatggctg tcaccaaact tcatgcttgc cgcggtaaag cccacggcat tgccgtttga
     6421 cgacatgggg ctagctacca agacggtgca tgaatcgatt ttgttaccgt cgatttcgcc
     6481 tttgaattgt tttgcaccca acaaagtggc cggatatgtg gttacttggc ttgtttcaaa
     6541 catttttcaa tttccttcag ttgtttaaaa aatcggatat ttgacgttcc tcgtactcaa
     6601 tatccttgat gtgttgccgt tccctgtctt gaggaaatgc gtattctctc tcttcaatca
     6661 aatcatcaaa cagtgtttca atgttcaatg cgttaatggc tttctgttct tcgtggatat
     6721 actggaattt ctgcgtttgg tttttgcaat cgtattgctc aggctgtaaa ccttttggat
     6781 aaccttcaac gcctttcacc agctcatcaa ctatcctgct atcgtcccac cctatatcgc
     6841 ggaggaaatt aaccatcttg ccgacttgat tccttgcatg aaaaagctta acgtcgaata
     6901 tcagattaac gtttttaact tttacttcca tgcgcttggc ttctgtcttg aatatcgcct
     6961 tacaaatcgg atatgcgccg cctagatacg accccgaata aagtaggata tccaagggta
     7021 tttctatatc ccccgccctg aattctgttt caaacctgac ccaagggcta tcaacatctc
     7081 caaactgcct gcctttctcg taaacacggg taaattttga atttccgcgc ttgccgatgt
     7141 aaaaggtttt tccactgccg tcttcattgc gccatgccgt gccgcgacat tcgctttttg
     7201 gccgcatatt gtgtacgtcg taatgtccgt tatcgtggtc taacattgcc tgatcgggtg
     7261 tgtactctcc gttgaaaaaa tcatgggcta catcgacacg ggttattttc gggcggacgc
     7321 atttacttaa gaattcatgt aggcggtttt cccaaccggg taaagcagcc atgcagcctg
     7381 taccgttcaa ttcaaccaac atcgtttcac gttgaccgcc gtaatgaacc tttccgtatt
     7441 caacgttatc gggcccgagt tgataacagc tttgatagaa aaactttcct ttaaacggta
     7501 attttctggt aatgccgaat cccaagattt cttcaagcag ctcgctatac tggacaacga
     7561 attccgtatc tgatactaag ccctttccgg ttactttcgt catggaattt tcatgtatcg
     7621 tgaaagtgat ttggtctatg aacgctccgt catccctgcc acgccttaac ggtatttcta
     7681 taaatctgcc tttgccgtcc gatacaaaat gactgaaata ctcgaattga aattcttggt
     7741 tttctgtttt ttccgcaccc ttcggatttg gggttttatt ttgctccccc cctattagcc
     7801 taggggggca gcctacggcg gttgccgcag ccccgccgtc cgctaacgcg tccgccatgt
     7861 ccgcggacac cgc
//
LOCUS       DAWKOD010000090         7868 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000090 DAWKOD010000000
VERSION     DAWKOD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.090"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1337)
                     /locus_tag="WJK73_001417"
     CDS             complement(<1..1337)
                     /locus_tag="WJK73_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ7557892.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1411..2751)
                     /locus_tag="WJK73_001418"
     CDS             complement(1411..2751)
                     /locus_tag="WJK73_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ7557893.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPMQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRLVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADAQAWSGLLVGSIACYGILPRFLAWVVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            2894..3520
                     /locus_tag="WJK73_001419"
     CDS             2894..3520
                     /locus_tag="WJK73_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ7557894.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3584..5002)
                     /locus_tag="WJK73_001420"
     CDS             complement(3584..5002)
                     /locus_tag="WJK73_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7557895.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5329..6057
                     /locus_tag="WJK73_001421"
     CDS             5329..6057
                     /locus_tag="WJK73_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ7557896.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6116..6604)
                     /locus_tag="WJK73_001422"
     CDS             complement(6116..6604)
                     /locus_tag="WJK73_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ7557897.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAIVLMCRPRRKAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(7362..7826)
                     /locus_tag="WJK73_001423"
     CDS             complement(7362..7826)
                     /locus_tag="WJK73_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ7557898.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2038 a         2111 c         1971 g         1748 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcgat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc ggcgagcagg
      241 gtcaaggttt cagggtcggt gtgcagggtt tggcggccag cgagtttgtc ggaaatggtg
      301 cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccagttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtaaag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcaat taaaaagtcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aactcgtccc tgtatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcgccg tggctgccga gaaactgttg cagccgttcg
     1081 atgccgtctg aacgattgtc cgtatggttt tccagccatt ccagcacgcc gcccgcgtct
     1141 tcgagtccgg gcgtgtcgta caggaaaacc agcgtgtctg cgccgtcgct gatggcggct
     1201 tcttcgacat gacgcgtggt cgatggggcg tttttgactt cgccgaaacc gctgtcgcgc
     1261 aaaagggtac gcaggagcga ggttttgccg gtgttggtgt gtccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacggtct tttttcagaa
     1381 tggcggctta acagaacatt tcaagtgagt ttattggtct ttcaaacgcc cttcctgcgc
     1441 cgccctgtca ggctcaagcc acgccgcgcc gcattcggcc agcgcgttac gccaatgttc
     1501 cagcttttcc gaaaggtcgt ctgaaagccc ctgttccgcc aaaagctgca ccaccgcgcc
     1561 gccctgcgcc gcttccgaaa gtcggacgat ctgccgcaac acgccgcggt cgggcacagt
     1621 ttgggcgcgc acgccgataa gcagttgcgc cggtttctgc ttcagctctg tctccagcgc
     1681 ggcaacctgt tcccgattgg tggcaacgcc cttatccagc cattcctgcg ccagcctgcc
     1741 ctcgaaccat tcgccgtcct gccactcggt ctccagcatg accgcccatt tcggcgcatc
     1801 gttcaagacg attttcggcg aaacggcgga cacggtttcc cgacgcgtat ccgcatcggt
     1861 aattttgttc tgccagcggc ggatgaccgc ctgataatag ggtttttcca aatccaagcc
     1921 gtttttgctt gttttcaaaa ggattttaca taccacccaa gccaagaagc gcggcaggat
     1981 gccgtagcag gcgatactgc cgaccagcag ccccgaccaa gcctgcgcat cggcaatatt
     2041 gccgttcaga cgaccttcaa ctaccgcccg cgcatcaggg acagggaaac cgagtttcga
     2101 cggcagccat gccaacattt ccaccagccg taccgaagcg gcattgctca acagcgtgct
     2161 ttcccagttg aacgtatatt ggcgcaccaa aagcagcaac aataccgaca ccagcattcc
     2221 gagcagcgtg cagagccaca ggctgtgcga cgttgcgcct attttccaac gtaccgaagg
     2281 ttgccgccac tcgtccgcat acagccgcaa caccgcctga tttacagggt ctttgccccg
     2341 aaaccacgtc gccggactgc tgaaaaaacg ccccactttc acgcgcagga acaacattgc
     2401 caaccatact gccagcatca gcgtattcat gcccaacacg cccgccaaaa ccaaaaagaa
     2461 attcagaccc tgattgtcca ttagaagata agtacctgaa aaaccggtaa aaaacgcaaa
     2521 cgtcgccgcc gccacccaca accagaacga ccccgcacgc acacgttcca atgtgtcccg
     2581 cagcatacgg ttcctatcga tcatctccgc ccgacggatg attttttcct ccgtactgcc
     2641 gtccacgcgg cgcaaagcct ccgtcgcctg catgggatcg ccgctgaaaa taaaaccgcc
     2701 ttcgtccaaa atacggatca gctcaaccag ttttcgggat ggattcaaca taaaatgccg
     2761 tctgaaaata aaaaacagat tttaacacac gcattttcaa gaatattcac agtgtaggca
     2821 aagagtaaat ctcacacaga ggcaaaagta tcggcgtaaa ctgactgcct ctactttccc
     2881 gaaagattgt gcgatgtata caggcgaacg cttcaatact tacagccatt tgagcggttt
     2941 gattctggcg gcggcaggtt tggcgctgat gttgctgaaa accataggac acggggacgg
     3001 ctaccgtatc ttcagcgtat cggtttacgg catcagcctt cttctgctct atttgagttc
     3061 ctcgctgtac cacggaattg cagccggaaa actgaaaagc attttgaaaa aaaccgacca
     3121 ctgcatgatt tatgtcctga ttgccggaag ctacacaccg tttgcactgg tttctttgag
     3181 aaacgggccg ggctggacgg tattttcact gtcctggctg ctggcggctg caggaatcgc
     3241 acaagaactc accatcggac ggaaaagcga aaaacgactg ctgtctattg cgatttatat
     3301 cgtaatgggc tggatggtct tggcggtaat gaaatccctg acagcctcac tcccgccggc
     3361 aggactggct tggctggcgg caggcggtat gctgtacagc gtcggcattt actggtttgt
     3421 aaacgatgaa aaaatccgac acgggcacgg aatctggcat ctgttcgtat tgggcggcag
     3481 catcacccaa tttgtcagtg tgtacggtta tgtaatctga atgccgtctg aaaaacaaaa
     3541 cctcccgttc ctgaagattg ggaggttttc tgtttgccgg acatcagccc ttgtcgtgga
     3601 actcgtggaa ttcatactga taggacaaat cccgacccgc ttttttctgt gccaaataat
     3661 catcataaat ggcgcggatt tccttacgca acaaaaacag ggctatcagg ttggggataa
     3721 ccataaaacc gttgaacata tccgacagac tccaaaccaa atcgactttg ccgagcgtac
     3781 ccaaaacaat ggcaagcaga accaatgcgc gatagatgcc caagtgtctt cccctgaaaa
     3841 gaaaacggat attggactcg ccgaaataat accaaccgat gatggtggtg aaggcaaaga
     3901 aggtcagaca cacggcaagc aattgcgaac cgaagcccgg aaatgccttg ttaaaggcaa
     3961 attgagtaac cgccgcgccc tgttcgcccg aaaggttggc atcggtcagc aggataatca
     4021 atgccgtagc cgtacatacc aaaatcgtat cgataaacac accgacaaat gccgccatac
     4081 cttgctgcac agggtgcttc acatccgcag tcgcgtgggc gtgcggagtc gaacccatac
     4141 ctgcttcgtt ggaaaacaga ccgcgcgcca cgccgaaacg tatcgcttcg cgcataccga
     4201 tacccgcagc accgcccaaa acggcttcgg gattgaaggc ggcggtaaag atgtggttga
     4261 acatcggcac aatatggtcg gaaaattcaa acaggataac gacggcgcac aaaatataaa
     4321 caaccgccat aaacggcacg acaaattggg caatattggc aatacggttc acgccgccaa
     4381 tcacaaccat gcccgcaagg acggcaagca caataccgac tgccaaagaa ggcacatcaa
     4441 atgcaatggt aacggcagaa gcaatggagt ttgcctgtgt cgcattaccg ataaagccca
     4501 atgcgataat caacgcaatg gaaaagaaac cggacaaaaa acgcgccgcg cccctgccga
     4561 ttttcggagt cagaccgtgg gtgatgtaga acgccggccc gccgatgtat ttgccgtggc
     4621 tgacgacgcg gtatttctgc gccagcagtg cctccgcaaa aatcgtggac atccccaaaa
     4681 cggcagaaac ccacatccaa aaaatcgcgc ccggcccgcc tgcggtgatg gcggtcgcca
     4741 cgccggcgac gttgcccgta ccgatttgcg cagatatggc aaccgccaac gcctgaaact
     4801 gcgataaaga cttgtcgtct ttatcgcctt tggcaaacaa gccgccgaat acggatttga
     4861 atcccgcgcc cagcttggta atctgcggcg caccaagata cagcgtaaaa aacaggccga
     4921 tacccaaaag cgcgtaaatc agcaggtagt cccaaaggaa ccgattgact gtacccacca
     4981 gaacagacaa tatattttcc ataaaataaa ccttatctta caattaaaat gactgccttc
     5041 caaaagacat tccaataagg aaacacggcg agcagaccgt atttgccgca acagatgcct
     5101 taaattgtca acaatcgggg agaagctgcg ccatcatacc gtaaaatatc gttaattaaa
     5161 acatttcttt atttttaagc ggaaagcgga ggaaatcgct ttcagacagc atagacaacg
     5221 gcacggcata aaacaggata ttttgggtac ttgcaactta tgttaaaatg ccgaccgtaa
     5281 aaaatctgac aaaaacagat taattatttg aaataagaaa ggaaatttat ggcaggccat
     5341 agcaagtggg caaatatcca gcataaaaaa gcccgtcagg atgccaaacg cggcaaaatc
     5401 tttacccgtt taatcaaaga aatcaccgtt gcggcgcgta tgggcggcgg cgaccccggt
     5461 tcaaatccgc gcctgcgcct agctttggaa aaagcagccg aaaacaatat gcccaaagac
     5521 aatgtgcaac gcgccatcga taaaggtacg ggcaacttgg aaggcgtgga atacatcgaa
     5581 ttgcgctacg aaggctacgg catcggcggc gcggctttga tggtggactg cctgaccgac
     5641 aacaaaaccc gcaccgttgc ggacgtacgc cacgcgttta ccaaaaacgg cggcaacttg
     5701 ggtaccgacg gctgcgtggc gttcaacttc gtgcatcagg gctatttggt attcgaaccc
     5761 ggcgttgacg aagacgcgct gatggaagcg gctttggaag ccggtgcgga agacgtggtt
     5821 accaacgacg acggttccat cgaagtcatt accgcgccaa acgattgggc gggcgtaaaa
     5881 tccgctttgg aggcggcagg ttacaaatcc gttgacggcg acgttacgat gcgcgcccaa
     5941 aacgaaaccg aactctccgg cgacgatgcc gtcaaaatgc aaaaactgat tgacgcgctg
     6001 gaagacttgg acgacgtgca agacgtttac acttccgccg tattgaatct ggactgatac
     6061 gcagcacagc agacatacaa taaaatgccg tctgaacctt tcagacggca ttgatttatt
     6121 tagcccttgc ccacgcccac caaaccgtca gggcaaccgc cagaaaatac gccacactcc
     6181 aaacaggcaa agcaaccccc agcagataat ccggttcggc acaatttccg aacccgcgca
     6241 cgacaggctc gaaccaatca aacaaaggcc agcccttcaa tcgaaacgtc cacggcgcac
     6301 cgcacgaagg agccgtaccc ggcggcagcg actgcagcca caactgatat gccgcaacag
     6361 aaatacccgt aacggcggga atgctgataa agacagcacc gaacaaaccg cctgcctttc
     6421 ttcttggtct gcacatcagg acaattgccg cacacaatgc ggttgccaag acgcacaacc
     6481 gctggctgat acacaaaacg caaggctcca tacccaaaac atactgtgcc gccaaagaac
     6541 cggcaaatgc acagaccgaa acggcaaaca gcagccaaac ggcttttcta aataacgggg
     6601 tcattttctc aacacaccaa tcaaaatacc gatatgccga ttttgctgga tatatcccga
     6661 gatggcaagg gacgaatcga caccggcgca ggtgcggacg atttcggttt tcccgatata
     6721 cacaagacct gacaagggac aaaacggcga tttgcccgca caatgcccgc aaaaaaatac
     6781 atccggagga tttgaatttc ggcaaattta gcgaatcaaa agtttatttc aatgaaatta
     6841 tatgattttt ttgaataagc ggattgacag gtttttgaag gaatttgtta ccggatagcc
     6901 atcgggcaag ttttttgcaa aatttgaatc ggtcgggtaa attttcaaaa aataattgac
     6961 agcggataag aaacggcgga taattcccgc cgtcgagttg cttgatgcag cttgattttt
     7021 ctcctctatt tctcctttgt agacttggca cacattcaac tggatgtgtg cattttttta
     7081 tctccgcttt ttttgaaatt taattacttt tattgtttga aattccattc tttaagaaat
     7141 ttaacaagaa aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg
     7201 gttcgagtag tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt
     7261 ctattgaatg tgtgcatttt tttatctgaa gcaacaagcc tctgtgcgtg atgttgttat
     7321 gtttcattta ggtgtcaaac cgcatacccg gtctgaaata ttcaatccaa atccaaaacc
     7381 ggattttctt tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt
     7441 tgcaatagga tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta
     7501 atcagatagt cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc
     7561 acttcttttt tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca
     7621 aaaaccaata gtggtttgcc caacagatgg ggattgagat gggcgtgata gccggaaata
     7681 taatgttccc gctccaaacg gcgcaccctc tctgtaacgg gcgtggtgga caagcctacc
     7741 ttctcggcaa gctccgtcat cgggatgcgg gcattctgtt ggaggatctt aagaatgcgg
     7801 aaatcgattt tatctagttc tttcatttgg attaccttgt atttattatt gattttaaca
     7861 aatagagt
//
LOCUS       DAWKOD010000091         7766 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000091 DAWKOD010000000
VERSION     DAWKOD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.091"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..536)
                     /gene="dksA"
                     /locus_tag="WJK73_001424"
     CDS             complement(120..536)
                     /gene="dksA"
                     /locus_tag="WJK73_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ7557899.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(683..1474)
                     /locus_tag="WJK73_001425"
     CDS             complement(683..1474)
                     /locus_tag="WJK73_001425"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ7557900.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1523..1930)
                     /locus_tag="WJK73_001426"
     CDS             complement(1523..1930)
                     /locus_tag="WJK73_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ7557901.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSECVRW"
     gene            complement(1930..2616)
                     /locus_tag="WJK73_001427"
     CDS             complement(1930..2616)
                     /locus_tag="WJK73_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ7557902.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2736..3779
                     /locus_tag="WJK73_001428"
     CDS             2736..3779
                     /locus_tag="WJK73_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ7557903.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3942..5168
                     /locus_tag="WJK73_001429"
     CDS             3942..5168
                     /locus_tag="WJK73_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7557904.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5454..7604
                     /gene="yccS"
                     /locus_tag="WJK73_001430"
     CDS             5454..7604
                     /gene="yccS"
                     /locus_tag="WJK73_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ7557905.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETADDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALNTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1902 a         2596 c         1693 g         1575 t
ORIGIN      
        1 tttctgctaa acgctgcacc acctccaata atcccttcct ccccctcctc tgactggtgc
       61 gcctttgtga atatgccgtc tgaaactcgg ggactcagac ggcattttgt ttgccgccat
      121 cagtcggcaa actgtttttt catcctctct cggcgttctt gggactcaac agataaagtg
      181 gctgtcgggc gtgccagcag ccgcttcaga ccgataggct ctcccgtatc ggcacagaat
      241 ccataatccc cttcatcaat attgcggatg gtcgcctgta ttttactgag aagttttcgt
      301 tcccgatcgc gggtacggag ttccaatgcg tactcttctt cctgtgtggc acggtcggca
      361 ggatcggggg ctgattcgtg ttcttggaga tgccctgtcg tagcggaagc attttcgatg
      421 agttcgtctt gcatttttac cagcaattcg cggaaaaaag ccaaatgctc gtcattcata
      481 taatcgtctt ccggcccgtt ccaattcaaa atatcttgtt ctgtcagctt tgccataatg
      541 tatatcctct cggttcaccg aaaaaaacaa agcaccacaa agtggcttta ttcctttgat
      601 ttttataagg cttattgcct agggcgtttt tgaaaacgcg catcataaca cgttttggat
      661 tggttttttt attttcttta cattattgat attgccgttc catttcctgc gaacggcgca
      721 cacaggcaca aacgccctcg cttatggctt cggcgacacg atgccgcctg aaagcttcca
      781 cggcttcgtg ggttgtcccg ccttttgacg ttacattttt ttgaagcttc tcgaaatctt
      841 cacccgtctg ctcggcaagg gcaaccgctc ctttaaacgt tgccagactg agcgcgcgtg
      901 cttctgccat atcaaaccct tgtcggatgg cggcattttg caatgcgtcc agcagataaa
      961 acacatatgc cggcccgctg ccgctgatac cggtaatgct gtgcatttgc gcttcatctt
     1021 ccaaccaaac agtcaaaccg actgatttca tgattcgatc ggcaatcctg cggtctgttt
     1081 ccgatacttc cgcttcggca tacataccgg atacgcccag cccgattttt ccgggtgtat
     1141 tcggcataac ccggacaatg cggcgtgttc ccccgaggta acggctgagc gtatcgaccg
     1201 gcaatccggc tgcgacagaa agcaccaatg cgccgttggt gcggatattt ttgcacgcgg
     1261 cttccatatc ctgcggcttg acggcaagga ttaaaacatc gtcggaatga agctccggca
     1321 aggtttcaga agtttcgacc cccaactctt tttgcaaacg ttcgcgcttt tccgcacccc
     1381 ggttggcaat atggatgcgg taaccgcctt gtttgaccaa tccgcccgca acggcagccg
     1441 ccatattgcc gccgccgaga aaataaacat tcattatttc taccctattc aaaattacct
     1501 gactaaattc attcccaatt ttttaccatc ggacacactc ggacagaccg ttgcgccgca
     1561 acccctgctt tttcaaacaa tcaaaacctt cctgtttgaa cgtctcaatc aaaggtttgc
     1621 cgtcacgcgt acgccaatcc aacccgcagg cttcaaactt ccctattttc ctgtaaaagt
     1681 aagattgttg tgcggattca tattcctttg ccctgacgga acgattgccg tctgaaagcg
     1741 gtatctcgta gtcctctaaa gacggcggac tctcgccgcc aatatcccaa aaatcgatgg
     1801 cggccggttt tctcggctta caccagccgg acagggacga acactcatac caacccgcca
     1861 accggcagcc tgcaaccgta aaggcaagca acgcagccgt tgccgtatat tttattgccc
     1921 gaatttccat catccccttt tcccgaaaat cgcgctgccg atacggacgt gtgtcgcacc
     1981 gcactcaatg gcggcaggca tatcgtccga catccccata gacagcacgt ccgccttaac
     2041 gccagccgta ttgaggtcgg caagcaactt ttgcatggtc tgaaactggg ttttcaactc
     2101 cgtttcactg ctgttggctt tggcaacgca catcagtccg cgcacgacaa ggttgggcaa
     2161 cttcgccact tcgaccgcaa gcgcgaccgc ttcttcggga gcaacaccgt gctttgccgc
     2221 ctcgcctgca atattcacct cgatacacac ctgcaaaggc ggcattgaag aaggacgctg
     2281 cccactcagc cggacggcgg tctttagacg gcatacggta tgcacccaat gcgcgcgttc
     2341 ggcgacaaac ttggttttgt tggactgcac atcgccgatg acgtgccaca cgatgtcggt
     2401 caaatccgcc aactcttccg ttttgccgta ccactcctga atatagttct cgccgaaatc
     2461 acgctgtccg gcggcgtaaa cttcgcggat gccgtctgaa gggaaagtct taccgacggc
     2521 aatcaggctg acggaatgcg gcttccttcc cgcctgcaga accaattttc caatacggtc
     2581 ggacacctcc cgataacgtt cttgcaacac cgtcatagat tatcccctaa ttaaaaatga
     2641 ttaaacagtt gaaacccctc cagtcagggc ggtacaatca aggttgttag aaccattcca
     2701 accaatcgaa acattatact aaacagagcc gcattatgca gattaccgac ttactcgcct
     2761 tcggcgctaa aaacaaagca tccgaccttc acctgagttc gggcatatcc cctatgattc
     2821 gggttcacgg cgatatgcgg cgcatcaacc ttcccgaaat gagcgcggaa gaggtcggta
     2881 atatggtaac ttcggtgatg aacgaccacc agcggaaaat ctaccagcaa aacttggaag
     2941 tcgacttctc gttcgaactg cccaacgtcg cccgattccg cgtcaacgcc ttcaacaccg
     3001 gccgcggtcc cgccgccgta ttccgcacca ttcccagcac cgtcttatcg ctggaagaat
     3061 tgaaagcccc gagcattttc caaaaaatcg cagaatcgcc gcgcggcatg gtattggtta
     3121 ccggccctac cggttcgggc aaatcgacca cgcttgccgc gatgatcaac tacatcaacg
     3181 aaacccagcc ggcacacatc ctgaccatcg aagacccgat cgaattcgtc caccaaagca
     3241 aaaaatccct gattaaccag cgcgagctgc accagcacac cctcagcttc gccaacgcgc
     3301 tgcgttccgc attgcgcgaa gaccccgacg ttatccttgt cggcgagatg cgcgaccccg
     3361 aaaccatcgg cttggcactg accgccgccg aaaccggaca cttggttttc ggcacgctgc
     3421 acacgaccgg cgcagcaaaa accgtcgacc gtatcgtgga cgtattcccg gcgggagaaa
     3481 aagaaatggt gcgctctatg ctgtccgaat cgctgaccgc cgtcatctcc caaaacctgc
     3541 tgaaaacgca cgacggcaac ggccgtgtcg cctcgcacga aatcctgatt gccaaccccg
     3601 ccgtccgcaa cctcatccgc gaaaacaaaa tcacgcagat taactccgtc ctgcaaaccg
     3661 ggcaggcgag cggtatgcag acaatggacc aatcgctgca atcgctggtg cgccaagggc
     3721 tgatcgcacc ggaagtcgca cgcagacgcg cgcaaaacag cgaaagtatg agtttctgac
     3781 acacaaccgc cttccggcca taccggcggg aaaacaaggc gcaaacacgc gaggcgggac
     3841 gcagcatccc gcccggctac ctttccgaac aagaagcgtc cgccttcctg ttgaaacctg
     3901 ccgccgcaaa ctgcaaggct taaaccgaaa agaagctaac gatgaatacc gataacctgc
     3961 acgacatctt ggacgaaatg gttcaagtgt attctcaaaa aaaacaaagc cgatccgaaa
     4021 ccccggccga aatcggcgca cacttccacc cgctgctcga ccgcctgtgc gaaaccgcag
     4081 aagcacaaaa cgcgtccgac atccttatca gcaaaggatt cccgccctcg ttgaaaatca
     4141 acagcgcatt aaccccgcag ccgcaaaagg cgctgacggg cgaggaaacc gccgccatcg
     4201 ccgcatcgac gatgaacgcc gaacaatcgg aaatattccg gcgcgacggc gaaatcaact
     4261 actccgtcca gtcgcgcagc ggcacgcgct accgcgccaa cgcctaccac agccaaggca
     4321 gcgcaggttt ggttttgcgg cgcatcaacc acgtcatccc gcaaatgcag gaattgggcc
     4381 tgcccgaaaa actcaaagac ctcgccgtcg caccgcgcgg gctgctgatt atcgtcgggc
     4441 ctaccggttc gggcaaatcc accacgatgg cgactatgct cgaacaccgc aacaaaaccc
     4501 tgcccagcca tatcgttacc atcgaagacc cgattgagtt tatctacaaa ccgcgccgct
     4561 gcatctttac ccagcgcgaa atcggcgtcg acaccataaa ctggcagacg gcggtacaaa
     4621 acgctatgcg ccaatccccc gacgtggtct gcatcggcga agtccgcagc agggaaagta
     4681 tggaatacgc gatgcagctc gcccaaaccg gccacctgtg catttttacg ctccacgcca
     4741 acaccgcgcc gcagtcgctc gaacgcatac tcaacttcta ccccaaagaa cagcacaacc
     4801 aaatactgat cgacatcgcc ctcaacctga ccggcatcat ctgccaacgc ctcgccctca
     4861 aacaagacaa aacgggcagg acggcggttg tcgacttgct catcaacacg cccgccatcc
     4921 aagacttcat cctgaagggc gacctgatga acatcagtaa aatcatggaa accgccaaaa
     4981 ccgacggaat gcagacgatg gatcaaaacc ttttcgaact gtaccgtcac ggcatcatca
     5041 gttacgaaga agccctgcgc cagtccgttt ccgccaacaa cctgcgattg cacatccaac
     5101 tgcacaaaga aggcaaaacg cccgaactcc tttacgacag ggtcaacggt ctcaacctca
     5161 tttcctgatc cgcaaaaccc aatgccgtct gaaaaccgca tccccgtttt cagacggcat
     5221 gattttatcc gccccattca tgtgctacac tttatagtgg attaaattta tagtggatta
     5281 acaaaaatca ggacaagacg acgaagccgc agacagcaca aatagtacgg aaccgattca
     5341 cttggtgctt cagcacctta gagaatcgtt ctctttcagc taaggcgagg caacgccgta
     5401 ctggtttttg ttaatccact atattcactt cataacaata aaccggtaaa accatgaaaa
     5461 ccccactcct caagcctctg ctcattacct cgcttcccgt tttcgccagt gtttttaccg
     5521 ccgcctccat cgtctggcag ctaggcgaac ccaagctcgc catgcccttc gtactcggca
     5581 tcatcgccgg cggccttgtc gatttggaca accgcctgac cggacggctg aaaaacatca
     5641 tcgccaccgt cgccctgttt accctctcct cgctcacggc acaaagtacc ctcggcacag
     5701 ggctgccctt catcctcgcc atgaccctga tgaccttcgg ctttaccatt ttaggcgcgg
     5761 tcgggctgaa ataccgcacc ttcgccttcg gcgcactcgc cgtcgccacc tacaccacac
     5821 ttacctacac ccccgaaacc tactggctga ccaacccctt catgattttg tgcggcaccg
     5881 tactgtacag caccgccatc atcctgttcc aaatcatcct gccccaccgc cccgtccaag
     5941 aaagcgtcgc caacgcctac gacgcactcg gtggctacct cgaagccaaa gccgacttct
     6001 tcgaccccga tgaggcagcc tggataggca accgccacat cgacctcgcc atgagcaaca
     6061 ccggcgtcat caccgccttc aaccaatgcc gttccgccct gttttaccgt ttgcgcggca
     6121 aacaccgcca cccgcgcacc gccaaaatgc tgcgctacta cttcgccgcc caagacatac
     6181 acgaacgtat cagctccgcc cacgtcgact accaagagat gtccgaaaaa ttcaaaaaca
     6241 ccgacatcat cttccgcatc caccgcctgc tcgaaatgca gggacaagcc tgccgcaaca
     6301 ccgcccaagc cctgcgcgca agcaaagact acgtttacag caaacgcctc ggccgcgcca
     6361 tcgaaggctg ccgccagtcg ctgcgcctcc tttcagacag caacgacaat cccgacatcc
     6421 gccacctgcg ccgccttctc gacaacctcg gcagcgtcga ccagcagttc cgccaactcc
     6481 gacacagcga ctcccccgcc gaaaacgacc gcatgggcga cacccgcatc gccgccctcg
     6541 aaaccggcag cctcaaaaac acctggcagg caatccgtcc tcagctaaac ctcgaatcag
     6601 gcgtattccg ccatgccgtc cgcctgtccc tcgtcgttgc cgccgcctgc accatcgtcg
     6661 aagccctcaa cctcaacctc ggctactgga tactactgac cgcccttttc gtctgccaac
     6721 ccaactacac cgccaccaaa agccgcgtcc gccagcgcat cgccggcacc gtactcggcg
     6781 taatcgtcgg ctcgctcgtc ccctacttta ccccctccgt cgaaaccaag ctttggatcg
     6841 tcatcgccag taccaccctc tttttcatga cccgcaccta caaatacagc ttctcgacat
     6901 ttttcatcac cattcaagcc ctgaccagcc tctccctcgc agggttggac gtatacgccg
     6961 ccatgcccgt acgcatcatc gacaccatta tcggcgcatc ccttgcctgg gcggcagtca
     7021 gctacctatg gccagactgg aaatacctca cgctcgaacg caccgccgcc cttgccgtat
     7081 gcagcaacgg cgcctatctc gaaaaaatcg ccgaacgcct caaaagcggc gaaacggccg
     7141 acgacgtcga ataccgcgcc acccgccgcc gcgcccacga acacaccgcc gccctcagca
     7201 gcaccctttc cgacatgagc agcgaacccg caaaattcgc cgacagcctg caacccggct
     7261 ttaccctgct caaaaccggc tacgccctga ccggctacat ctccgccctc ggcgcatacc
     7321 gcagcgaaat gcacgaagaa tgcagccccg actttaccgc acagttccac ctcgccgccg
     7381 aacacaccgc ccacatcttc caacacctgc ccgaaaccga acccgacgac tttcagacag
     7441 cactgaatac actgcgcggc gaactcgaca ccctccgcac ccacagcagc ggaacacaaa
     7501 gccacatcct cctccaacag ctccaactca tcgcccggca gctcgaaccc tactaccgcg
     7561 cctaccgcca aattccgcac aggcagcccc aaaatgcagc ctgaaaaagt ttcggcattt
     7621 tgtaagagag ggcagattgt cagacaggtt gcaagatagt ggataaggct ttgcgcaggg
     7681 taaatgcgta gcaactgaac cgtcattccc acgaacctac atcccgtcat tcccacgaaa
     7741 acagaaaacc aaaaacagaa acctaa
//
LOCUS       DAWKOD010000092         7660 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000092 DAWKOD010000000
VERSION     DAWKOD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.092"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            189..1724
                     /locus_tag="WJK73_001431"
     CDS             189..1724
                     /locus_tag="WJK73_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7557906.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1721..1813
                     /locus_tag="WJK73_001432"
     CDS             1721..1813
                     /locus_tag="WJK73_001432"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ7557907.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1849..3093)
                     /gene="lysA"
                     /locus_tag="WJK73_001433"
     CDS             complement(1849..3093)
                     /gene="lysA"
                     /locus_tag="WJK73_001433"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ7557908.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3104..3274)
                     /locus_tag="WJK73_001434"
     CDS             complement(3104..3274)
                     /locus_tag="WJK73_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7557909.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3345..3668
                     /gene="cyaY"
                     /locus_tag="WJK73_001435"
     CDS             3345..3668
                     /gene="cyaY"
                     /locus_tag="WJK73_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ7557910.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3699..4118
                     /locus_tag="WJK73_001436"
     CDS             3699..4118
                     /locus_tag="WJK73_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ7557911.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4140..4646
                     /gene="luxS"
                     /locus_tag="WJK73_001437"
     CDS             4140..4646
                     /gene="luxS"
                     /locus_tag="WJK73_001437"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ7557912.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4792..7587
                     /gene="polA"
                     /locus_tag="WJK73_001438"
     CDS             4792..7587
                     /gene="polA"
                     /locus_tag="WJK73_001438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ7557913.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         1752 a         2046 c         2097 g         1765 t
ORIGIN      
        1 atttttcgtt ttcaagcctt caccgcttgc catcagcgtt aaattttttt acgataagca
       61 catagattgt aaacaatcgg ccacaagccg gtttgttttt tcagaagaca ttatccctgt
      121 cagacgctat ttctatatat ttcgcctata atggcttgtt tttaataaat aattcaagag
      181 gtatcaacgt gtctgattcc aagacgaaag aacgcgccac attcggcacg cgccgcgcgt
      241 ttatgattgc cgccatcggg tccgccgtcg gcttgggcaa tatttggcgt ttcccctata
      301 ttgcttttga aaacggcggc ggcgcgttca tcctgcccta tctggtcgcg cttctgacgg
      361 cgggcatccc gctgctgctg ctcgattatg ccatcggcca ccgttaccgt ggttctgcgc
      421 ccttggcttt ccgccgcctc ggacgatggt ttgagccggt cggctggtgg aacgtgatga
      481 ccaatatcgt catctgcatc tattacgcgg taattatcgg ttgggcggca agctatacct
      541 attattcggt caacgccgcc tggggtgcgg atccgcaggg ttttttcttt aaggacttcc
      601 tgcaaatggc gggcccggaa gccttgggtt tggattttgt cggcaaagtc gccggtcctt
      661 tggcgggcgt gtgggttttt accgccgcca ttatggcgtt gggcgtgcaa aagggcgtgg
      721 cgcgcgcctc gtcgttcttt atgccgctgc ttttggtgat gtttttgatt atggtcggca
      781 tttcactaac cctgccgggt gcggcaaagg gcttggacgc attgtttacg cccgactggt
      841 cgaaactcgc cgattccaag gtctgggtgg cggcatacgg gcagattttc ttttcgcttt
      901 ccatctgctt cggcattatg gttacctatt cttcttattt gaagaaaaaa accgacttgg
      961 gcggaacggg gctggtggtc ggttttgcca acagcagctt tgaactgctc gcgggcatcg
     1021 gcgtgtttgc cgcattgggc tttatggcgc aggcgggcgg taaggcggtc aacgaggttg
     1081 cctcaggcgg catcggtttg gcgtttatcg cctttccgac cattatcaac caggcaccga
     1141 tgggctggct gatcggcata ttgtttttcg gttcgctggt attcgccggc gttacgtcga
     1201 tgatttccat ccttgaagtg attgtggcgg cgattcagga caagctgaac atcgggcgcg
     1261 tcaacgccac gctgctggtc tgcattccga tgggcattgt ttccacgctg ctgttcggta
     1321 cggcgacggg gctgccggtt ttggacgtga tggacaaatt cgtcaacacc tacggcattg
     1381 ttgccgccgg ctttgtttat gttgccgcca tcatcatcag cggcaggctg ccggaattac
     1441 gcaagcacct gaacgctttg tcctccatcc gcatcggcgg cttgtggacg gtctgcgtcg
     1501 tggttaccgt cgtgatgctc ggctatatgc tgtttaaaga taccagcggc ctgatggaga
     1561 aaaattacga aggttatccg gatggtttcc tcagtatttt cggctggggg atgtcggcgg
     1621 cgttggtcgt gttcgggctg ctgctgtcgt tgctgccttg gaaacacggt caggatttca
     1681 acgtcaaaga cgaacacgaa catgaacaag gagaagaaaa atgagtactt ccgccattgt
     1741 gatgatgatt gtctcaatcg tgataatctg gggagggctg ctgctttccc tgttaaggct
     1801 gccgaacgag taagccttta gagcgttaaa aatgccgtct gaaccgcttc agacggcatt
     1861 ttgatgttcg gcttgcaggc aggcgagttc gtttgccatt tgctgttcta aggtttcgcg
     1921 tcggcggatg agtttgtatc cgccaccgtt aaccaacact tccgccgcac ggttgcgcgc
     1981 gttgtaattg ctcgccatac tggctccgta tgcgcccgcg ctgcggataa gcagcaaatc
     2041 gccttcttcg caggcgatgg tgcggtcttt gccgaggaag tcgccggttt cacaaatcgg
     2101 gccgacgatg ttggcggtca gaggctcaat gtctttggtt tcgaccgcct cgatgtgatg
     2161 ataggcatca tatagggcgg ggcgcattaa atcgttcatc gccgcatcga ccatcacaaa
     2221 gtttttctct tcgccgtatt tgacgaattc gacacgcgtc aacaacgcgc ctgcgttgcc
     2281 gaccaagctg cggccgggct caagaatgag tttcagacgg cgtgtgccga tcagtttttg
     2341 aaccgcttgg gcatacgcgc ccaaatcagg cactttttcg ttttggtaaa caatgccgac
     2401 gccgccgccc aagtccaagt gttccaacac aatgccttcg gcggcaagcg cgtcaaccaa
     2461 aatcaaaatg cgctcgcagg cttcgaccag cgggcttaag tcggttagtt gggagccgat
     2521 gtggcagtcg atgccgatga ttttcaaatt gggctgttgt gcggcgtggc ggtaggcttc
     2581 gagcgcgtcg gcgtaggcga tgccgaattt gttggctttc agacctgtgg agatgtaggg
     2641 atgggttttc gcatcgacat cgggattgac gcgcagggag acgggcgcgg ttttacccaa
     2701 acgcgcggca actttctgaa tacggtcgat ttcggggatg ctttccatat tgaagcattt
     2761 cacgcctgca ttcagcgcga actcgatttc cgcctcgctt ttgcctacgc ctgaaaatat
     2821 ggtttttgcc gcgtcgccgc ctgccgccaa aacgcgtgcc aattcgccgc cggacacaat
     2881 gtcaaaaccg ctccccaaag aagcaaagtg tttgatgatg ctcaggttgc cattggcttt
     2941 aaccgcatag cagacgagcg gggagagctc ggcaaaggca gtttggtagt gttcaaatgc
     3001 ttcggtcagc gcggattggc tgtacacata aagcggtgtg ccgaatgctt cggcaaggcg
     3061 ggggtagggg acttgttcgc aaaatagggt catattttcg ttttcatttt tgggttggag
     3121 gggcggattg cggtttgcct tgaagctgca aaccggtttg gattacgccg aaacgcgcct
     3181 tgtcgccttc tttgggcagg tagaggtcgc ctttgtaacc gcaggccgag agcaggaggg
     3241 cggttgccgc cgcaaaaaat acgccgtatt tcatcggtaa acttccttca taagcgcgaa
     3301 tgtggcaaga ttcggcatct taaacaaaaa acacgcaaaa agctatgatg accgaaagcg
     3361 agtttatccg cgcgagcgaa gcattatttg aacacatcga agaccaaatc gacgaaaacg
     3421 gctgggattt cgactgccgg tttgccggaa acgtcctgac catcgaagcc ggagacggca
     3481 cgcaaatcat cgtcaaccgc cacacgccca atcaggaatt gtggattgcc gcaaaaagcg
     3541 gcggctacca tttcgccgaa caaaacggca aatggctggc aacgcgcgac agccgcgatt
     3601 tttatgatgt tttgaacgaa gcactgagcg cggcttcggg cgaagcggtt gagattgagg
     3661 aattgtgatt caatttgata taaaaagatt gttacccaat ggcacaatta cctctatatc
     3721 ggactgccga aatcggaaat tttactgtcg gcacgcctaa agttttagaa tcattttcca
     3781 aacatatccc ttatggtgtc gtctttgaag acgacggcga cacgggctac ttctatgctg
     3841 cttcgcaaga cgggatttta gatgccttgc acatctacaa tgtcgaagat gtatccgaca
     3901 aacatatccc caatcatgtc ttgattttat gggatgatgc ctgcaccata gccgcattgt
     3961 gtgtcaacga ctacattcat gccgtctatg attttgtcga acaggcagga tattgccgca
     4021 acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga aaaccgcgtc ttggatgatg
     4081 aattgctgga caaaatccta tcccgagaac ctacataacc tactcaaaag gataacaaaa
     4141 tgcccctgtt agacagtttc aaagtcgacc acacccgtat gcacgcccct gccgtacgcg
     4201 tggcgaaaac catgaccacg cccaaaggcg ataccattac cgtattcgac ctgcgctttt
     4261 gcgttcctaa caaagaaatc ctgcctgaaa aaggcataca cacgctggag catttgttcg
     4321 caggctttat gcgcgaccac ttaaacggca acggcgtcga aatcatcgac atttccccga
     4381 tgggctgccg caccggtttt tatatgagcc ttatcggcac gccttccgaa cagcaggtcg
     4441 ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt caaagaccaa agcaaaatcc
     4501 ccgagttgaa cgaataccaa tgcggcactt atcaaatgca ctcgctcgcc gaagcgcaac
     4561 aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa caaaaatgaa gagctgacgc
     4621 tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc cgtctgaaca agggtttcag
     4681 acggcatttg ccttttccgt tataatctgg ggttgtgcgg gggcgggttt taagccggca
     4741 tcgtccttcc ctattttttt ctgtccctta tcggttttaa gcgggttttt tatgtccaac
     4801 agacctacac tcctcctcgt tgacggatcg tcctacctct accgtgcgta tcacgcgatg
     4861 gggcaaaacc tgaccgcacc cgacggtgca ccgacgggtg cgctgtatgg cgtattgaat
     4921 atgttgcgcc gtttgcggtc ggaatatccg cacgattatt gcgcggtggt ttttgatgcg
     4981 aaaggcaaaa atttccgcca tcaaatgttt gaagaataca aggcgacgcg cccgccgatg
     5041 cccgacgatt tgcgtccgca ggcggaagct ttgccggatt tggtgcgcct gacgggctgg
     5101 ccggtattgg tgattgggca ggtggaagcg gacgatgtga tcggcacgct ggcaaaacag
     5161 ggggcggaac atggtttgcg agtcattgtt tcgaccggcg ataaggacat ggcgcagttg
     5221 gtggatgagc gcgttacgct ggtgaacacg atgagcggag aaacgctgga tattgaaggc
     5281 gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg attatctcgc gctgatgggc
     5341 gacaaggtgg acaacgtgcc gggcgtggaa aaatgtggcc cgaaaacggc agtgaagtgg
     5401 ctggaagcct acggctcgtt gcaaggcgtg atagaacacg cttcggaaat caagggcaaa
     5461 gtgggcgaga atctgcgtgc cgccctgccg cgcctgccgc tgtcgtatga tttggtaacg
     5521 attaaaaccg atgtggactt gcacgccgag ctttcagacg gcctcgaaag cctgcgccgt
     5581 accgcgccga aatgggcgca gctggttgtc gatttcaaac gctggggctt ccgcacctgg
     5641 ctgaaggaag cggaaagccg tatgcacgaa gcggcggacg gcgatttgtt cggcagcgat
     5701 acgataggcg aacaggcggc gttggatatg gaaacgtcgt ctgaaaggct acctgaaaaa
     5761 gccgttgccc ccgaaaagct ggattatcag gccgttacca ccgaagcgca gtttgccact
     5821 ttgttagaca aattgtcgca ggcggaaaaa atcggcatcg acacggaaac cacttcccta
     5881 gacgcgatga acgccgcgct ggtcggcatc agcatcgcgt tccaagcggg cgaagcagtt
     5941 tacatccccg taggacacag cctgaccgcc gcgccagaac agcttgattt gcaagacgta
     6001 ttaggccatc tgaaaccgta tttggaaaat cctgccctga aaaaaatcgg gcaaaacctc
     6061 aaatacgacc aacacgtttt cgccaattac ggcatcgccc tgaacggcat cgccggcgac
     6121 gccatgctcg cttcctacat catcgagagc catctcggac acggcttgga cgaattgtcc
     6181 gaacgctggc tcggcttgga aaccattacc tacgaatcgc tgtgcggcaa aggcgcgaag
     6241 caaatcggtt ttgccgatgt cgccatcggg caggcgaccg aatacgccgc ccaagacgcc
     6301 gatttcgccc tgcgcctcga agcgcacctg cgcgcgcaaa tggacgaaaa acagcttgaa
     6361 atgtatgaaa aaatggagct gcccgtcgcg caggtattgt ttgaaatgga acgcaacggt
     6421 gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg aactcggcgc cgagctgatg
     6481 aagctcgaac aagaagccta cgccgccgca gggcagccgt tcaacctcaa ctcgcccaaa
     6541 cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga caaaagggct gaaaaaaacc
     6601 gccaagggcg gcatttccac caacgaggcc gtgctcgaac agctcgcgcc cgactacccc
     6661 ttgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac tcaaatccac ctacaccgac
     6721 aaactgcccg acatgatttc tccccgggat aaccgcgtgc ataccaccta cgcccaagcc
     6781 gtcgccatta caggacgctt ggcaagcaac aaccccaacc tgcaaaacat ccccatccgc
     6841 accgccgaag gccgccgcgt gcgccgcgcc tttaccgcgc caccgggcag cgtcatcgtt
     6901 tccgccgact attcccaaat cgaactgcgc atcatggcgc acctctctgg agacaaaacc
     6961 ctcattgccg cattccaaaa cggcgaagac gtacaccgcc gcaccgccgc cgaagtgttc
     7021 ggcactgcgc ccgaaaacgt ctcgcctgag caacgccgct acgccaaaac catcaacttc
     7081 ggcttaatct acggtatggg gcaatacggc cttgccaaat cattgggcat cgacaacctt
     7141 tccgccaaaa actttatcga ccgctacttc gcccgctacc ccggcgtcgc cgaatacatg
     7201 cagcgcacca aagaacaagc cgccgcccaa ggcttcgtcg aaaccctgtt tggccgccgc
     7261 ctctacctgc ccgacatccg caacaaaaac gccaacgccc gcgccggagc cgaacgcgcc
     7321 gccatcaacg cccccatgca aggcaccgcc tccgacctta tcaaacgcgc catgatagac
     7381 gtgtcccgct ggctggtttc agacgacctg aagagcaaac tgattatgca ggtgcatgac
     7441 gaactggtgc tggaagtcgt tgaaaccgaa ctggattttg tcaaagaaaa actgccgcag
     7501 attatggcga aagtgggcgg cggattattg gatgtaccgc tggtggctga ggttggcgta
     7561 ggggagaatt gggaagaggc acattgattg aaaggtgtta ttatgctatc tttatttaaa
     7621 taaaatttaa tttttggtat attttttcta aatgttccta
//
LOCUS       DAWKOD010000093         7635 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000093 DAWKOD010000000
VERSION     DAWKOD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.093"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            7..1884
                     /locus_tag="WJK73_001439"
                     /pseudo
     CDS             7..1884
                     /locus_tag="WJK73_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            1946..3241
                     /gene="tyrS"
                     /locus_tag="WJK73_001440"
     CDS             1946..3241
                     /gene="tyrS"
                     /locus_tag="WJK73_001440"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ7557914.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSV
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALVAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPTFEVSESLNVVETLVKTGLASSNKEARGFVNNKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            3247..3465
                     /locus_tag="WJK73_001441"
     CDS             3247..3465
                     /locus_tag="WJK73_001441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557915.1"
                     /translation="MPQKCRHSRLRGNDGGGLFVSVYLVKKERFSLMLHQVWGDVSTH
                     STAPAARFCAFGAFGGGKFAYFSRVGRA"
     gene            3541..4461
                     /gene="ribF"
                     /locus_tag="WJK73_001442"
     CDS             3541..4461
                     /gene="ribF"
                     /locus_tag="WJK73_001442"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ7557916.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            4602..7391
                     /gene="ileS"
                     /locus_tag="WJK73_001443"
     CDS             4602..7391
                     /gene="ileS"
                     /locus_tag="WJK73_001443"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ7557917.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1776 a         2178 c         1978 g         1703 t
ORIGIN      
        1 aacattatga gccaagcctt accctaccgc ccggacatcg acacattgcg cgccgccgcc
       61 gttttgtccg tcatcgtgtt ccatatcgaa aaaaattggc tgccgggcgg atttctcggt
      121 gtcgatatat tctttgtgat ttcaggcttt ttgatgacgg agatcctcct tcgcgaaatg
      181 tcgggggggg ggaggttttc cctgaaggca ttttatatcc gccgcatcaa gcggattctg
      241 cccgcatttt tcgccgtatt ggcggcaacg ctgataggcg gcttcttttt attcaccaaa
      301 gatgatttct ttcttttgtg gaaatccgcg ctgaccgcct tgggtttcgc ctccaacctg
      361 tattttgcaa ggggcaagga ttatttcgat cccgcgcagg aagaaaagcc cttgctgcac
      421 atttggtctt tgtcggtcga agaacaattt tactttgtct ttccgatatt gctgctgctt
      481 gtcgcccgca aaagcccgcg cgtacagttc ggcttcctcg ccgcattgtg cgccttaagc
      541 cttgccgctt cctttatgcc ttccgcgttc gataaatatt acctgcccca cctgcgcgcc
      601 tgcgaaatgc tggtcggatc gctgaccgcc gtgtggatgc ggtaccggca acagcggaat
      661 ctcgccgtcg gcagatgctg tgccgccgcc ggcgcattgt tttcctcgtg catactgtcc
      721 gcctgcctgt ttgcctattc ggaacaaacc gcctatttcc cggggcccgc cgctttgatt
      781 ccctgtctgg ctgttgccgc gctgatttat ttcaaccatt acgaacaccc acttaaaaaa
      841 tttttccaat ggaaaatcac cgttgccgcc ggtttgattt cctattcgct ttatctgtgg
      901 cattggccga tattggcctt tatgcgctat atcggtccgg ataacctgcc gccttattcg
      961 ccggcggcgg cggtcgtcct gatattgctg ctttccctgt tttcttacca ctgcatcgaa
     1021 aaaccgttta aaaaatggca aggttcgttc gcacaatccg ttttatggat ttatgccttg
     1081 cctatgctca ttttgggggc gggctcgttt ttcgcgatga ggctgccgtt tatggcgcaa
     1141 tacgaccgtt tggggctgac gcgttccaac acctcctgcc acaacaatac cggcaaacaa
     1201 tgcctgtggg gggatacgga aaaacagccg gagctgctgg ttttgggcga ctcccacgcc
     1261 gaccattaca aaacattctt cgatgccgtg ggcaaaaaag aaaaatggtc cgccactatg
     1321 gtttccgccg acgcttgcgc ctatgtggaa ggctacgcgt cccgcgtgtt ccaaaactgg
     1381 gccgcctgcc gcgccgttta ccgctatgcc gaagaacacc tgccccggta ttcaaaagtg
     1441 gttttggcga tgcgctgggg cagctagatg cccgaaaaca gccgctccct tgcctatgat
     1501 gccggttttt tccaaaaatt cgaccgtatg ctgcacaaac tctcatccga aaaacaagcc
     1561 gtttacctga tggcggacaa cttggcctcg tcttacaacg tccagcgcgc ctatatcttg
     1621 tcttctcgca taccgggtta ccgccaaacc ctgcgcccgg acgacgaaag cactctgaaa
     1681 gccaatgcac gcatcaggga attggcagcc aaatacccca acgtctatat tattgatgcc
     1741 gccgcctata tccccgcaga ttttcaaatc ggcggattgc cggtttactc ggacacagac
     1801 cacatcaacc cttacggcgg cacggaattg gcaaagcgtt tttccgaaaa acaacggttt
     1861 ctcaatacgc gccataacca ttgattcgct taaatttgtt acaatcggcg gtttgcaaaa
     1921 acgctaattt ttttgaaaga gaccgatgag cgtcatccaa gacctgcaat cgcgcggcct
     1981 aatcgcgcaa acgaccgaca tcgaagcctt agacgctttg ttgaacgaac aaaaaattgc
     2041 cctttattgc ggcttcgacc cgaccgccga cagcctgcac ataggacacc tgctgcccgt
     2101 attggcattg cgccgtttcc aacaggcggg gcatacgccg attgctttgg tgggcggcgc
     2161 gaccggtatg atcggcgacc ccagcttcaa agccgccgaa cgcagcctca attccgccga
     2221 aaccgttgcc ggctgggtag aaagcatccg caaccaatta acccctttct tgagctttga
     2281 aggcggaaat gccgccatta tggcgaacaa tgccgactgg ttcggcagca tgaactgcct
     2341 tgacttcctg cgcgacatcg gcaagcattt ctccgtcaac gccatgctga acaaagaatc
     2401 cgtcaaacag cgcatcgacc gcgacggcgc aggcatttcc ttcaccgagt tcgcctactc
     2461 cctgctgcaa ggctacgact ttgccgagtt gaacaaacgc cacggcgcgg ttttggaaat
     2521 cggcggctcc gaccaatggg gcaacatcac cgccggtatc gacctgaccc gccgcctgaa
     2581 tcaaaaacaa gtgttcggcc tgaccctgcc tttggttacc aaatcagacg gcaccaaatt
     2641 cggcaaaacc gaaggcggcg cggtatggct gaacgcgaaa aaaacctcgc cctatcagtt
     2701 ctaccaattc tggctgaaag ttgccgatgc cgatgtgtat aaattcctga aatactttac
     2761 cttcctgtct gtcgaagaaa tcgatgccgt cgaagccaaa gaccaggcca gcggcagcaa
     2821 gcccgaagcg caacgcatcc tcgccgaaga aatgacccgc ctgattcacg gcgaagaagc
     2881 ccttgtcgcc gcgcagcgca tttccgaaag cctgtttgcc gaagaccaaa gcagcctgac
     2941 cgaaagcgac ttcgagcagc tggcactcga cggtctgccg acttttgaag tttctgagag
     3001 cctcaacgtg gttgagactt tggtgaaaac cggtttggcg tcttccaaca aagaagcgcg
     3061 cggctttgtg aacaacaaag cggttttgct caacggcaaa cctgccgaag ccaacaatcc
     3121 gaaccacgcc gccgaacgcc ccgacgatgc ctgcctgctg accgacgaac acaaacgttt
     3181 cggcaaatac accatccttc ggcgcggcaa acgcaaccac gcgcttttgg tttggaaata
     3241 atccgattgc cgcagaaatg ccgtcattcc cgcctgcgcg ggaatgacgg cggagggttg
     3301 tttgtctcgg tttacctggt taaaaaagaa cgattttcac tgatgttgca tcaggtttgg
     3361 ggcgatgttt caacacatag caccgcgcct gctgcgcgtt tttgtgcgtt tggcgcgttc
     3421 ggcggcggga aatttgccta cttttcccgc gtcgggcggg cgtaacgggc ggcacactgt
     3481 ctataaaccg caataccgtt tacaatgacc gcctgtttga ccacactccc gaacataaca
     3541 atgagaatca ggctggggcg gcacaacgcg cccgactttc cgcacggggc cgccgtaacc
     3601 ataggcaatt tcgacggcgt acacctcgga cacaaacaca tcctccaaaa actccgcctc
     3661 gaagccgacg cgcgcggact gcccgtcgtt gccgtcatct tcgaacctca acccaaagaa
     3721 ttttttgccg ccctcgcagg caaaaccccg ccctgccgca tcagccccct gcgtaccaaa
     3781 ctggaattgc tggaagggac gggctgcgtc gatgccgtct gggttttgcg tttcgatcaa
     3841 aatttttccg aaatatccgc gcaagggttt atcgaccgcc tgctgcgtca aaccttgaat
     3901 acgcgttatt tgctcgtcgg cgacgatttc cgtttcggtg cggggcggga aggctgtttt
     3961 gaacttttgg cacaacagcc cgatatgcag accgagcgta cgccttccgt catcgtcgaa
     4021 gacatccgca ccagcagtac cgccgtgcga caagcccttt cagacggcaa ccttgcctat
     4081 gcgaaaaaac ttttgggaca cgactacgtc ttgagcggca gggtggtgca cggcagaaaa
     4141 ctcggacgca ccttaaacgc cccgactgcc aacatccgcc tgccccgcca ccgttatgca
     4201 ctcggcggcg tgttcgtcgt cgaagcggac ggcgcgttcg gcacgcggcg cggcgtggcg
     4261 agcttcggct tcaatcccac cgttgatagc gactgttttc aaaagctgga agtccacctg
     4321 ttcgactttc aaggcgattt gtacggacaa cggttgaacg tccgcttcct gcacaaactg
     4381 cgcgatgagg aaaagtttga cggtatggaa gaactgaaaa ggcagattga agccgatatg
     4441 gaagccgcaa agtgttggta gaaaaacctt atacaaacca tccgattggg ctacaatcaa
     4501 ccttttaact gttcagacgg cataggggtt tcccgttgtg aaatactgtt tgaggcgcaa
     4561 tgccgtctga accgaaatat tgtaacaata gagattaaaa aatgaccgat tacagcaaaa
     4621 ccgtaaacct gctcgaaagc ccgtttccga tgcgcggcaa tcttgccaag cgcgagcctg
     4681 catggctgaa aagctggtac gagcaaaaac gctaccaaaa actgcgcgaa atcgccaaag
     4741 gtcgtccgaa gttcatcctg cacgacggcc cgccgtatgc caacggcgac atccacatcg
     4801 gtcacgccgt caacaaaatt ctcaaagaca tcattatccg cagcaaaacc caagccggtt
     4861 ttgacgcgcc ttatgtgccg ggttgggact gtcacggttt gcctatcgaa gtgatggtag
     4921 aaaagctgca cggcaaagac atgcccaaag cgcgtttccg cgaattgtgc cgcgaatatg
     4981 ccgccgaaca agttgcccgc cagaaaaaag actttatccg tctgggcgtg ttgggcgact
     5041 gggacaagcc ctacctgacc atggatttta aaaccgaagc cgataccgtg cgtatgctcg
     5101 gcgaaatcta caaatccggc tatctctacc gcggcgcgaa accggttcag ttctgcttgg
     5161 actgcggctc ttcattggcc gaagccgaag tggaatacaa agacaaagta tcgcctgcga
     5221 ttgacgtggg ctatccgttt aaagacaccg ctgcgcttgc cgctgcattc ggtttggcag
     5281 gcatcgaggg caaagcgttt gccgtcatct ggacgaccac gccttggacg ctgccagcga
     5341 gccaagccgt gtctgccggt gcggacgtgg tgtatcaatt aatcgatacg cccaaaggca
     5401 aattggtgct ggcgaaagat ttggcagaag acgcgctcaa acgttacggt ttttcagacg
     5461 gcattgctat tcttgccgaa accaccggcg acaagctgga aaacctgcac atgaatcatc
     5521 cgttcctcga acgcgatatt cccatgctca acggcgacca cgttaccacc gacgccggta
     5581 ccggtttggt gcatactgcg cctgctcatg gtttggaaga ctacgccgtc tgcaataaat
     5641 acggcatcga gctttacaac cccgtcaacg ccgaaggcaa atacatcagt gaaacgccgc
     5701 gcgttgccgg catgagcgtt tgggaggcca atcccgttat cctgcaatgg ttggaagaaa
     5761 tcggcaacct cttggcaagc agcaaaatcg aacacagcta cgcgcactgc tggcgtcaca
     5821 aaacgccgtt gatttaccgt gcgaccggcc aatggtttgt cggtatggat aaagcaggtg
     5881 ccgatggtaa aaccctgcgc gataaagcca tcaaagccgt tgatgacact gaatttttcc
     5941 catcatgggg tcgtgcgcgt ttggaagcca tgattgaagg ccgccccgac tgggtggttt
     6001 cgcgccaacg ctattggggc acgccgatga ctttctttgt tcacaaagaa acgggcgagc
     6061 tgcatccgaa ctctgccgaa cttttggaaa aagtcgcgca acgcatcgaa gaaaaaggca
     6121 tcgaggcttg gttctccctc gataagggcg aattgttgag cgcggaagat tgcgaacatt
     6181 acgacaaact ctccgacacg atggacgtat ggttcgactc cggttcgacc cattattccg
     6241 ttgtgaaaca gcgcgaagaa ttggactggc ctgctgactt gtacctcgaa ggcagcgatc
     6301 aacaccgggg ctggtttcag tcgtccatgc tgaccggctg cgcctcatca atgggtcgcg
     6361 cgccgtacaa acagctgctg acccatggtt tcgtggtgga tcaaaacggg cgcaaaatgt
     6421 cgaaatccat cggcaacgtc gtcgcgccgc aagaagtgta taacgagttc ggcgcggaca
     6481 tcctgcgcct gtgggcggca tccaccgatt acagcggcga attggcgatt tccaaagaaa
     6541 tcctcaagcg cgtaaccgaa agctaccgcc gtatccgcaa caccttgagc ttcctgtttg
     6601 ccaacctcag cgacttcaat cctattgaag atgccgtgca acaggcagac atggtggaaa
     6661 tcgaccgcta cgccgtggta ttggcgcgtc ggctgcaaga gcgtctggca ggcgattact
     6721 atccgcgtta tgccttccat tttgccgtga aagacattgt ttctttctgc tcggaagact
     6781 tgggcgcgtt ctacctcgat atcctgaaag accgcctcta caccaccaaa gccgacagcc
     6841 gcgcccgccg cagcgcgcaa accgccctgt accacatcac gcgcagtttg gttctcttga
     6901 ttgcgccgat tttgtgcttt accggcgaag aagcgtggga catcatcggc ggcggcgaag
     6961 aagacagcgt cctcttccac acctggcacg agttcccgac catcaacgaa aaagccgaag
     7021 ccgaactggt gaaaaaatgg acggcaatcc gcgaagcccg cgaagcggta accgccgcca
     7081 tcgagccttt gcgcgccgac aaaaccgtcg gttcgtcctt gcaagccgaa gccgaaatta
     7141 ccgcgccgga agaaatggcc ggctatctga atgctttggg cgaagaattg cgctttgctt
     7201 tgctggtgtc taaagcagaa gtgaaagtag gcagcgaact tgccgttgcc gctaaagcca
     7261 gtgatggtga aaaatgcgaa cgctgctggc actacacccg cgatgtgggc gcggttgcag
     7321 gctatgaaac cgtctgcaaa cgctgtgcag agaatgtcgg cggagaaggc gaaacgcgcc
     7381 attacgcctg ataaagtttg agcaaatgcc gtctgaaacc gccgaacagc atttcagacg
     7441 gcattttttg tgccgtgatt tgtctttata acggcggagg ggtttcaaga ttgcggtgtt
     7501 gtcggaatgc aactgaaccg tcattcccac gaaagtggga atctagaatc tcgaggtttc
     7561 agtcatttcc gatagattcc cgccgcgtcg tgggtctgga ttcccgcctg tgcgggaatg
     7621 acggatttcg agatt
//
LOCUS       DAWKOD010000094         7555 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000094 DAWKOD010000000
VERSION     DAWKOD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.094"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..419
                     /locus_tag="WJK73_001444"
     CDS             15..419
                     /locus_tag="WJK73_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7557918.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJK73_001445"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJK73_001445"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ7557919.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1383..1730
                     /locus_tag="WJK73_001446"
     CDS             1383..1730
                     /locus_tag="WJK73_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ7557920.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1735..2328
                     /locus_tag="WJK73_001447"
     CDS             1735..2328
                     /locus_tag="WJK73_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ7557921.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2392..3000
                     /locus_tag="WJK73_001448"
     CDS             2392..3000
                     /locus_tag="WJK73_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ7557922.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3008..3658
                     /locus_tag="WJK73_001449"
     CDS             3008..3658
                     /locus_tag="WJK73_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557923.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3698..4477
                     /gene="map"
                     /locus_tag="WJK73_001450"
     CDS             3698..4477
                     /gene="map"
                     /locus_tag="WJK73_001450"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ7557924.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4632..4931
                     /locus_tag="WJK73_001451"
     CDS             4632..4931
                     /locus_tag="WJK73_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7557925.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5169..5543
                     /locus_tag="WJK73_001452"
     CDS             5169..5543
                     /locus_tag="WJK73_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7557926.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5731..7197)
                     /locus_tag="WJK73_001453"
     CDS             complement(5731..7197)
                     /locus_tag="WJK73_001453"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ7557927.1"
                     /translation="MTEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTFRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1945 a         2286 c         1849 g         1475 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac aaacattata aattataaag gttaaaccga
     1381 ttatgcgcct aaaccacaaa cagggcgagg caggggaaga tgccgcgctt gccttcctcc
     1441 aatcccaagg ctgcacactg cttgcccgca actggcactg cgcctacggc gaaatcgacc
     1501 tgattgtcaa aaacggcggc atgattctgt ttgttgaagt aaaataccgc aaaaatcggc
     1561 aattcggcgg tgccgcatac agcatttccc catccaaatt attgaaactg caacgaagtg
     1621 tagagtatta tctgcaacag aacaggttga caaacgtacc gtgccgcctc gacgcggtac
     1681 tcatcgaagg cagccgcccg cccgagtgga tacagaatat tacaggttga cgatatgacg
     1741 acattacaag aacgcgttgc cgcccatttt gccgaaagca tccgtgccaa gcaggaagcc
     1801 ggaaaagtat tggtcgagcc gaccgtacag gctgccgagc tgatgctgca atgcctgatg
     1861 aacgacggca agattctggc gtgcggcaac ggcggttcgg ctgccgacgc gcagcacttc
     1921 gccgccgaaa tgaccggacg ctttgaaaaa gagcgcatgg aactcgccgc cgtcgccctg
     1981 accaccgaca catccgcgct gaccgccatc ggtaacgact acggcttcaa ccacgtcttc
     2041 agcaaacagg tacgcgccct cggacgcgcc ggcgacatcc tcgtcggcat ttccacttcc
     2101 ggcaattccg ccaacgtcat cgaagccatt aaagcggcac acgaacgcga tatgcacgtc
     2161 atcgccctga ccggccgcga cggcggcaaa atcgccgcca tactcaaaga caccgacgtt
     2221 ctgctcaacg tcccccatcc gcgcaccgcc cgcattcagg aaaaccacat cctgctgata
     2281 cacgccatat gcgactgtat cgactccgta ctgctggaag gaatgtaacc cttttcagac
     2341 ggcatggcgc aaagcaatgc cgtctgaaac gcccaagaaa ggaagccccc gatgaaaccc
     2401 aaaccgcaca ccgtccgcac cctgattgcc gccattttca gccttgccct tagcggctgc
     2461 gtcagcgcag taatcggaag cgccgccgtc ggcgcgaaat ccgccgtcga ccgccgaacc
     2521 accggcgcgc aaaccgacga caacgttatg gcgttgcgta tcgaaaccac cgcccgctcc
     2581 tatctgcgcc aaaacaacca aaccaaaggc tacacgcccc aaatctccgt tgtcggctac
     2641 aaccgccacc tgctgctgct cggacaagtc gccaccgaag gcgagaaaca gttcgtcggt
     2701 cagattgcac gttccgaaca ggccgccgaa ggcgtataca actacattac cgtcgcctcc
     2761 ctgccgcgca ctgccggcga catcgccggc gacacttgga acacatccaa agtccgcgcc
     2821 acgctgttgg gcatcagccc cgccacacag gcgcgcgtca aaatcgttac ctacggcaac
     2881 gtaacctacg ttatgggcat cctcaccccc gaagaacagg cgcagattac ccaaaaagtc
     2941 agcaccaccg tcggcgtaca aaaagtcatc accctctacc aaaactacgt ccaacgctga
     3001 ctcggcaatg ccgtctgaac cgccttcaga cggcattgcc cgacacccca aaagcacaat
     3061 caaaatggca aaaaaaccga acaaaccctt caggctgacc cccaaactcc tgatacgcgt
     3121 cgtattgctc atctgtatca ccgccatcgg cgcattggca ataggcatcg tcagcacatt
     3181 caacccgaac ggcggcaaaa ccctccaaac cgaaccgcaa cacaccgaca gcccccgcga
     3241 aaccgaattc tggctgccaa acggcgtagt cggacaagat gccgcccaac ccgaacacca
     3301 ccacgccgcc tcatccgaac ccgcacagcc ggacggcaca gacgaaagcg gcagcggact
     3361 gccgtcccct gccgcaccca agaaaaaccg ggtcaaaccg caacctgccg acacagctca
     3421 aaccgacagg cagccggacg acgccggaac acaagctgaa aacacactca aagaaacccc
     3481 cgtactgccc acaaacgtcc cccgtcccga accccgaaaa gaaacacccg aaaaacaggc
     3541 gcagcccaaa gaaacaccca aagaaaaaga aacgcccaaa gaaaaccata ccaaaccgga
     3601 caccccgaaa aacacgccgc ccaaacccca taaagaaatt ctcgacaacc tcttctgacc
     3661 cggcacggca ggcacacccg caatccaagg aagcattatg aacggcatca tcatcaaaac
     3721 ccccgaagaa atcgaaaaaa tgcgcgagct gggcaaactc gtcgccgaag ccctcgacta
     3781 catcggacaa ttcgtcaaac ccggcgtaac caccgacgaa atcgacaaac tcgtttacga
     3841 ctaccacgtc aacgtccaag gcggctatcc cgcccccctg cactacggca acccgcccta
     3901 ccccaaatcc tgctgcacct ccgtcaacca cgtcatctgc cacggcattc ccgacgacaa
     3961 gccgctcaaa gaaggcgaca ttatcaacat cgacctcacc atcaaaaaag acggcttcca
     4021 cggcgactcc agccgtatgt ttaccgtcgg caaagtctcc cccatcgccc aacgcctgat
     4081 cgacgtaacc cacgcctcca tgatggcggg catagaagcc gtcaaacccg gcgcgacact
     4141 gggcgacgta ggttacgcct gccaacaggt tgccgaaaac gccggctatt ccgtcgtaca
     4201 ggaattctgc ggacacggca tcgggcgcgg tttccacgaa gccccgcaag tgttgcacta
     4261 cggaaaaaaa ggacagggct ccgttctaaa accgggtatg atttttaccg tcgaaccgat
     4321 gatcaaccaa ggcaaacgcc acctgcgtat cctcaacgac ggctggacgg tggttaccaa
     4381 agaccgctcc ctctccgccc aatgggaaca cgaagtcttg gtgaccgaaa ccggctacga
     4441 aatcctcacc gtcagccccg cctccggcaa accctgaagc cggacgtatc cgccccataa
     4501 aaacaaacaa tgccgtctga aagaaacggc agatatgata tataatataa aaacaggctc
     4561 gacccggcac attacgaaaa caaagcaaat cggaatttgc cccgcaacca gacaaactta
     4621 aaggaagttt tatgaaaata tttgaaaata tagaagatgt taaagccatc cgtaaaaaga
     4681 ccgggctgaa ccagatagac ttctggggca aggtcggcgt tacccagtct ggaggctcgc
     4741 gctacgaaac cggccgcaaa atgcccaaac ccgtacgcga actgctccgc ctcgtccata
     4801 tcgaatgcat cgatttggcg aaagtcaaca aaaaagatat ggaaatcgcc gccctgttga
     4861 aaaaacacca tcccgacctg tatgccgagt tgtccaaaca gaccaagtcc gaaagaaaaa
     4921 aacaaagtta aaccgcaacc tccggatgcc cgaaggtttt tcatttccga aaaacgcaaa
     4981 caatgccgtc tgaaacaccg gacaggccgc cgtatcccgc ctgccgcccc tgcctcaaac
     5041 cgccgaatcg cccgaacccg cctttttaca aactttatcc aatttcctgt ttatttcagg
     5101 atacgccgac attagaatgt caaaccgtcc gaaacgggca aactccacat ccatccaaag
     5161 gaataaaaat gaaacttctg accaccgcaa tcctgtcttc cgcaatcgcg ctcagcagta
     5221 tggctgctgc tgccggcacg aacaacccca ccgttgccaa aaaaaccgtc agctacgtct
     5281 gccagcaagg taaaaaagtc aaagtaacct acggcttcaa caaacagggt ctgaccacat
     5341 acgcttccgc cgtcatcaac ggcaaacgtg tgcaaatgcc tgtcaatttg gataaatccg
     5401 acaatgtgga aacattctac ggcaaagaag gcggttatgt tttgggtacc ggcgtgatgg
     5461 atggcaaatc ttaccgcaaa cagcccatta tgattaccgc acctgacaac caaatcgtct
     5521 tcaaagactg ttccccacgt taatcaggca acaaaaaaca gcgttttcag aaatgaaaac
     5581 gctgtttttt tgaccgttcc attattcgca aaagggaaaa aacgattacc tgccccgtgt
     5641 atcaaaacct gccctgccgg atgaagggca taaccggcag ggacggcgtc aacaccatat
     5701 gggggtacgg cttttcttga aagattcggc ttaaatatcc aatactttcg cggtataggc
     5761 gataatttca tccgcccttt cagggttttc gttcaacttg atgccgtaac ccggtaccag
     5821 ctctttcaga cggtcttccc aagacggggc gcgctcgggg aagcattggt gcatcagccg
     5881 gatcatcagc ggcacagcgg tcgatgcgcc cggcgacgcg cccagcaatg cggcgagtga
     5941 gccgtcggcg tgggcgacaa tctccgtacc aaactggagc acgccgcctt tttcggagtc
     6001 ttttttaatg atttggacgc gttgccctgc ggtgatgagt tcccagtcgt cggggtttgc
     6061 ctcggggtag tattccagca gggaggcgaa gcgttcttct ttggttttac gcaattcgcc
     6121 cagcaggtat ttggtcagcg gcatattcgc ccagccggcg cgcagcatag gatagaggtt
     6181 gtccatatgg atggacagcg gcaaatccat aagcgagcct tgcttgagga agttggaacg
     6241 gaagcctgcg taagggccga acataaggtg gcgtttgccg tccacgttgc gtgtgtcgag
     6301 gtgcgggacg gacatcggcg gcgcgccgac ggaagcctgc ccgtacactt tggcgttgtg
     6361 ttgttcggca gtttcggggt tgctgttgcg gaagaacagg ccggacacgg ggaagccgcc
     6421 gtagcctttg ccttcgggga tgccggattt ttgcagcagg gtcagcgcgc cgccgcccgc
     6481 gccgaggaag aggaagcggg tacggaaggt gagctgcccg tcggggttgc gggtatcggc
     6541 ggttttgagc acccacgcgc cgtcggattc gcgtttgatg tcttcgacgt ggcggttgaa
     6601 ctcggttttt acgcccttgc cctgcaaata tttcaccatt tggcgcgtca gccgtccgaa
     6661 atcgacatcc gtgccttcgg cggagtagtt ggcggcgacg ggttggtttt cgtcccggcc
     6721 gcgcatcatc agcggagccc aatcggaaat tttgttccga tcggtggaaa attccatatt
     6781 ttcaaaaagt ttttgggttt taaacgcgtc ataacgtttt tgaagataag aacaatggtc
     6841 ttcattcatc accaaagaca tatgcggcac ggcattgatg aaggaattgt cttccaactt
     6901 gccttccgcg accagcgtcg cccaaaactg gcggctgaca tgaaactgtt cggcaatatt
     6961 gagggcgcgc gccggatcga taatcccatt tgcacccaac ggcgcatagt tcaattcgca
     7021 cagcgcggaa tgccccgtgc cggcgttgtt ccacgcgttt gacgattcca acgccacatc
     7081 ttccaagcgt tcaatcaggg tgatttccca agacggttcg agttctttga gcaaaacgcc
     7141 caaagtcgcg ctcataatgc cgccgcccac caagacaacg tctgtcgctt cagtcatggt
     7201 ttactcctaa aaaacaggca tcttctgccc ttatggttat ttgccgtact acaaacgcct
     7261 gaatcgcaaa agcagggaaa accggcaatg gtgtgtgtcc gagtatgctg tttcggggtt
     7321 ggaatgcgtt gcaagtatgg cttccgacac cgcttcaggg gcttgtaata tgttatcgtg
     7381 aatgtagtgg attttactgg gaaatgcaaa gtttttctgt cgcccgccaa gcccgggaac
     7441 tgcgaaatga aaaataaaaa tagttattta tctatatata tcaaattttt aatagataaa
     7501 aaaccaaaat tgtttatata ttaattttta aaagattgtc agcatattgc gttaa
//
LOCUS       DAWKOD010000095         7399 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000095 DAWKOD010000000
VERSION     DAWKOD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.095"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(144..566)
                     /locus_tag="WJK73_001454"
     CDS             complement(144..566)
                     /locus_tag="WJK73_001454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ7557928.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WJK73_001455"
     CDS             complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WJK73_001455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ7557929.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WJK73_001456"
     CDS             complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WJK73_001456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ7557930.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2912..4459)
                     /locus_tag="WJK73_001457"
     CDS             complement(2912..4459)
                     /locus_tag="WJK73_001457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ7557931.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4470..5003)
                     /locus_tag="WJK73_001458"
     CDS             complement(4470..5003)
                     /locus_tag="WJK73_001458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ7557932.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5008..5478)
                     /locus_tag="WJK73_001459"
     CDS             complement(5008..5478)
                     /locus_tag="WJK73_001459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ7557933.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WJK73_001460"
     CDS             complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WJK73_001460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ7557934.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WJK73_001461"
     CDS             complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WJK73_001461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ7557935.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6698..7051)
                     /locus_tag="WJK73_001462"
     CDS             complement(6698..7051)
                     /locus_tag="WJK73_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ7557936.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1946 a         1968 c         1679 g         1806 t
ORIGIN      
        1 tttcggttag gcggcaggct gccctattga ataccttaaa gcaggctatg cctgccaacg
       61 ccatatccaa acacagtctt taatttaaat ccggaaaata aaaagcacga ccaaacggtc
      121 gtgcttttcc aaaccaaaca agtttatttc ttgtgcgaac ggatatagtc caaagttttg
      181 agctgtgcaa tcgcagcagc caatgcttta tgtgcttccg ccaaagcctt atcgtcttta
      241 gcttgggaaa tgcccgcttc tgcggctttt ttcgcctctt ccgcacgtgc ctgatccatc
      301 tccgcactgc ggacggcaac atccgccaag acagttactt tatcaggctg tacttccaaa
      361 acaccgccgg aaacagcaac caaaacctct ttatcctcgc ccggaacggt caaacgcaaa
      421 gcccccggcc gcaccaaact cataatcggc tcgtgtcgcg gataaatacc gagttcgccc
      481 tgtacagtcg gaacaacgat aaatgttgcc tcgcctgaat agattttctg ctcgctactt
      541 accacctcaa cttccatgat gctcatgccg acctcctcag tttaaggttt tcgctttctc
      601 aaccgcttct tcgatgctgc cgaccatata gaatgcctgc tcgggcagat ggtcgtattc
      661 gccgttcaag atggctttga agccggcaat ggtatcgcgc agggcgacat atttgcccgg
      721 agaacctgta aacacttcgg caacgtggaa cggttgggac aggaagcgtt ggattttacg
      781 cgcacgcatt acggtcagtt tgtcttcgtc agacaattcg tccataccca agatggcgat
      841 gatgtcgcgc aattctttgt atttctgcag ggtggactgt acgccgcgcg ccacgtcgta
      901 gtgttcttga cccaagacca tcggatccag ttggcgtgaa gtagagtcaa gagggtcaac
      961 tgccgggtaa atacccaaag aggcaatatc acggctcaat acgacggttg cgtccaagtg
     1021 ggcgaacgtt gttgccggag acgggtcggt caagtcgtcc gcaggtacat atacggcttg
     1081 gatggaagta atagaaccgg tttgggtaga ggtaatacgc tcctgcaaac gacccatttc
     1141 ttcagccaat gtcggttggt agcccactgc agaaggcata cggcccaaca atgcggatac
     1201 ttcggtacca gccagggtgt aacggtagat gttgtccacg aagaacaata cgtcgcggcc
     1261 tttgccgttt tcgtcttttt cgtcacggaa gtattccgcc atggtcaaac cggtcaatgc
     1321 gacgcgcaaa cggttgcccg gaggttcgtt catctgaccg taaaccattg ccactttatc
     1381 caatacgttg gaatctttca tctcgtggta gaagtcgtta ccttcgcggg tacgctcacc
     1441 cacgcctgcg aacacggaca agccgctgtg cgctttggcg atgttgttga tcaattccat
     1501 catgttcacg gttttaccca caccggcacc gccgaacaga cctactttac cgcctttggc
     1561 aaacggacac agcaagtcaa tcactttaat gcccgtttcg agcaattcgg ttgtggaaga
     1621 cagttcgtca aacttagggg cggcttggtg gatggcacgg ctcttgtcgg tatcaatcgg
     1681 acctgcttcg tcaacaggcg ttcccaatac atcgacaatg cgtcccaacg tacctttacc
     1741 taccggcaca gtaatgggcg caccggtatt gctcacagtc atgccgcgtt tcaaaccgtc
     1801 cgagctgccc atcgcaatgg tacggactac gccgtcgccc aaaagctgtt ggacttccaa
     1861 agtcagaccg ttttcgtcta atttcaaagc gtcgtaaacg cgcggaatca tgtcgcgtgg
     1921 aaattccacg tcaacaaccg caccgataat ttgtacgatt ttgccttggc tcattatcgt
     1981 atcctaattt ctgtacagga ttcagacggc atcagacagc cgccgcacct gctacaattt
     2041 ctgacaattc cgtggtaatc gcagcttgac gcgatttgtt atataccaga cgcaactctt
     2101 tgatggcatt gcctgcattg tctgttgcag ctttcatggc aaccatgcgg gctgcctgtt
     2161 cggatgccat attgtcgctc aacgcctgat aaaccacaga ctctaaatag cggcgaacca
     2221 gatattccaa cactgcaagt gcagtcggtt cgtagcggta ttcccagctg aacggtgatt
     2281 tgggagctga atcgccaatc acgttctcac cgataggcag caatacttcc attctcggtt
     2341 cttgacgcat ggtattgaca aaacccgaat acaccagatg gattctgtca atttcatgtt
     2401 tctcataccg ttggaagagt tctgtcaaag gtccgagcag catttccatt tttggggtat
     2461 cgcccaaatt tacggcactg gcaaccacat tcagaccaat gctctgacac gccatcagac
     2521 ctttactgcc aaggcatacg atttcctctt caataccttg attccgatac tcttgaactt
     2581 gtgccaaaaa ctttttcagc acgttggcgt tcaaaccgcc acacaaaccc ttatcagacg
     2641 taatcaaaat aaaaccgaca cgtctaatct cccgatgaga ttccagtaac ggaataccat
     2701 gatcggtatt ggtttgcgca agatggctca tcaccatacg cactttttcg gcatacggac
     2761 gcgccaaacg catccgttcc tgagtcttcc gcattttaga ggttgacacc atctgcatcg
     2821 ctttagtgat cttttgggta ttctgaacac tgcggatttt ggtgagaatc tcttttccta
     2881 ctgccatttc agactccttt cacttcaagc cttatgcctg ataggcgtaa gaagatttga
     2941 aggatttcat ggctgcttca agcgttttct cgctctcgtc ggacattgca cctgaagcat
     3001 tgacggcttc caaaacttcc ggatgttggg tacggacaaa gctcaaaaat tcagattcaa
     3061 aagccagagc tttggcaacc ggaacatcag aatacgaacc gttgttgatt gcccaaaggg
     3121 tcaaagccat ttcagccgta ttcaacgtac tgaactgttt ctgtttcatc agttcggtta
     3181 cgacttcgcc atgctccaat tgtttgcgcg tagcttcatc caaatcggat gcaaattgcg
     3241 agaacgccgc caattcacga tattgtgcca acgccaaacg gataccgcca cccagctttt
     3301 taatcacttt ggtttgtgca gcaccgccta cgcgggatac ggaaataccg gcattgattg
     3361 caggacggat accggcgttg aagaggtcgg tttccaagaa aatctgaccg tcggtaatcg
     3421 aaatgacgtt agtcggaacg aaagcagata cgtcgcccgc ttgggtttcg ataatcggca
     3481 aagcagtcag agaaccggtt ttgcctttta cttcgccgtt ggtcaatttc tccacttcgt
     3541 gttcattgac acgtgctgca cgttccaaca gacgggagtg caggtagaac acatcgccgg
     3601 gataggcttc gcggccgggc ggacggcgca aaagcaggga aatttgacgg taagccacag
     3661 cctgtttgga caaatcgtca taaacaatca aggcatcttc gccgcggtcg cggaagaatt
     3721 cacccatcgt acaaccggag taaggtgcga tatattgcaa tgccgccgct tcagatgcag
     3781 ttgcagcaac cacaatcgtg tgttccatcg cgccatgctc ttccaatttg cggaccacgt
     3841 tggcaataga agatgctttt tgaccgatag cgacatagat acagataaca cccgtacctt
     3901 tttggttgac gatggcatcc agcgctacgg ccgttttacc ggtttgacgg tcgccaataa
     3961 tcaactcacg ctgaccgcga ccgacaggaa ccatagagtc aatcgccttc agaccggttt
     4021 gcatcggctg gtcaaccgat ttgcgcgcaa tcacgcccgg tgcgattttt tcgatagggg
     4081 cggtcaaagt tgtattaatc gggcctttgc cgtcgatagg ccgacccaat gcatcaacga
     4141 cgcgtccgac cagttcgcgt ccgaccggca cttccaagat acgaccggta caggtaaccg
     4201 tgtcgccttc tttaatatgt tcgtactcgc ccaacactac ggcgccgacg gagtcgcgct
     4261 ccaggttcat cgccaagccg aaagtgttac ccgggaattc gagcatctca ccttgcattg
     4321 catctgacaa accatagatg cgaacgatac cgtcagttac cgaaatcacc gtaccacggg
     4381 tacgcacttc ggcatttaca gacagatttt cgatcttggc tttaatcaaa tcgctaattt
     4441 cagcaggatt aagctgcatg aaaactctcc taattcgtca tagtcgtgta caaggcactc
     4501 aatttgcctt gtacagacaa atccaaaacc tgatcaccca cttcaacttt tatgccgcca
     4561 atcagctccg gttcgatttc gacagagatt ttcagctcgc tgtcgaaacg cttattcagc
     4621 atttgcgcca actcgccgac ctgtttgtcg gtcaacggat aggcactgta aatgacggca
     4681 gatttgatat ggttgaatga taaggtcaag tcttgatatt gagcatatac ttccggcaat
     4741 atcgacaaac gtttctgccc ggccaagacg ataacaaagt ttttcaactc cttgtctttc
     4801 aaaccgacca aatcgatgag gatatctgct ttttctgaag cattcgtttc aggacggtca
     4861 atcaatgaag ccaccttccc ttcctgaaca accgccgcaa gtttttccag tccgcccaac
     4921 caagactcaa tttggctttt ttcctgagcc agaccgaaca atgcctttgc ataaggtctg
     4981 gcaatcgttg cgaactctgc cataagatta cagctcctgt ttcagggtat cgagcagttt
     5041 tgcgtgtttg gaagcatcga cttcgctgcg caaaatagat tcggcacctt tgacagccaa
     5101 cacggcaacc tgctcgcgca gggattcgcg tgcgcggaac aattcctgct ccacatcggc
     5161 ctttgcctga gctgcaatgc gcgccgcctc ggaagaagcc tgttctttgg cttcttcgac
     5221 aattttggcg gcacgttttt cggcgttggc aaccatttcg gaaacctgat tacgcccttc
     5281 tgccaagagt tctgcaacct ttttttcagc ctgctcgaaa tcgcttttac cacgctcggc
     5341 agctgccaag ccttcggcga cttttgcggc acgctcatcc aaagcttttg caatcggcgg
     5401 ccacacgaat ttcatggtaa accataccaa accgaaaaag acgatgattt gagcgaataa
     5461 tgttgcattg atattcacgt tacttaacct tcgtattggg gttaatcaaa caggctgcgc
     5521 ctgtacggaa cggacgaatc cgtcctgatt atgcacctgc aaacgggtta acgaaggcga
     5581 acagcagtgc aatggcgaca ccaatcaaga atgcggcatc aatcaaaccg gcaatcagga
     5641 acagtttggt ttgcagcgga ccgatcagtt cgggctgacg ggcagaagac tccaaatatt
     5701 tagaaccgac cattgcgata ccgatagagg cacccagtgc gcccaatgca acgatcaaac
     5761 cacatgcgat agcaatcaaa cccattttaa actccttaaa gaaacaaagg ttaaactaca
     5821 aaaacaaact acttaggaaa atcagtgcgc atcatgtgcc tgtccgatat agacgaacgc
     5881 caacgccatg aaaataaacg cctgcagggt aatcaccaaa atatggaaaa tcgcccatgc
     5941 caaaccggca ataatgtgga atacaaacag aatcggatcc atgacttcga cgctgccgga
     6001 agccgcccaa gcaccgccaa gcaaggctat caacaagaat accaactcgc ctgcatacat
     6061 attgccgaac aaccgcatac cgtgggatac ggttttagaa agaaactcga ccaaattcag
     6121 caggaagttc gcaggtgcga gttttgcacc gaacggcgcg ctgaacaact cgtgaaacca
     6181 tccgcccaat cctttgattt tgatgttgta atagatacaa atcagcaaca cgccgacagc
     6241 gagtgccaaa gtggtgttca aatcggcagt cggtacgacg cgcaacaggg cgtgatggtt
     6301 gccggtaatg ccctgccata ccatcggcag caaatcgacc ggcagcatat ccatcgcgtt
     6361 catcaggaaa atccagacaa acagcgtcag acccaacggc gcgacggctt ttctagactt
     6421 ttcgttgtga atgatgctct tacacatatc gtccacaaac tcaaacaaga tttccacggc
     6481 ggcctggaaa cgtccgggaa cgcctgccgt cgcttttttt gcaccgcgcc acaacaggaa
     6541 gctgccgatt acgcccaaca ggacggcaaa aaagacggca tcaaggttaa taaacgaaaa
     6601 atcagcaatg tttttcagtc cctgaccctg agtaacatcc gacaaactgg tcaagctctg
     6661 caagtggtgc ttgatgtagt cggcagcggt aatggtttca cctgccataa tctttcactc
     6721 tcaacaatac taaaaaaacc aaatggctga caccgagcag ccccatcaga aacggggcga
     6781 acaccagcga ttgatgccat acagcaaata cggcgagcat ggacaacagc gacagcacta
     6841 cttttaaaat ctctccgaag acgaacatcc tgctttgcag gaaggggttt cccctgaaaa
     6901 gttttaaaag taaaactgca acaaacgtgg gaagcaggta ggacaaaccg ccaccgaccg
     6961 ccgaaaggaa tccggcaaaa ccccatacgg caaaggcaac tgcggcgcat atggacaata
     7021 cggcggattg taggatgata atctgcttca taaagggaat gtttccgcct cgaatttgag
     7081 gcgcggctaa tataatttag aagccttatt acgtcaagca atatgttaat ctttgtgaaa
     7141 caacgtatcc caatccgccg cgctcgccgc ctgaataacg gcgacaggtg tcattctaac
     7201 acacattaca tataattaca ggatattaag gagtttgtcc gcaatttctt tacactttta
     7261 atgttcttac gtgatttgtt tgctttacgt ggaaataata aaaaatcaac gcgaaattgt
     7321 agcagtttat cggtcggatt gtcggcagtt tggggaattt gctcaataaa taaaaggtcg
     7381 tctgaaaata ttttcagac
//
LOCUS       DAWKOD010000096         7201 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000096 DAWKOD010000000
VERSION     DAWKOD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.096"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..954)
                     /locus_tag="WJK73_001463"
     CDS             complement(85..954)
                     /locus_tag="WJK73_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7557937.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WJK73_001464"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WJK73_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ7557938.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WJK73_001465"
     CDS             complement(1963..3060)
                     /locus_tag="WJK73_001465"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ7557939.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WJK73_001466"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WJK73_001466"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ7557940.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WJK73_001467"
     CDS             complement(4392..5291)
                     /locus_tag="WJK73_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ7557941.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WJK73_001468"
     CDS             complement(5395..6048)
                     /locus_tag="WJK73_001468"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ7557942.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WJK73_001469"
     CDS             complement(6127..6780)
                     /locus_tag="WJK73_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ7557943.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7058..7142
                     /locus_tag="WJK73_001470"
     tRNA            7058..7142
                     /locus_tag="WJK73_001470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7092..7094,aa:Leu,seq:caa)
BASE COUNT         1476 a         1718 c         2122 g         1885 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagct cgtccgagcc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atggatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgtcccc aaaggcgagg aaaagcgggc ggttcggctg gtgggcaata catggtttgg
     3301 accggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcaccggc
     3361 gtggcggatt tttttcgccc attcctgcgg attttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atccatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgcct ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgctttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctggatcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatga atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttt gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aacaggcgcc gacggtgcag atggcatacg
     6121 gttttatcag agcagggtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggt tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agccgacgcg gatgcacttg aagaagcgac ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggcaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtctgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gatacagtaa gattaaaata ataaaaattc
     6961 tatttaaaat caaaaataaa ataaaaacag ataaaataat ttcaaaaaaa aggtttacag
     7021 aatttcaaaa gccattataa tgacgacttc ttcaccagcc caggtggcga aattggtaga
     7081 cgcaggggac tcaaaatccc ccgccgcaag gtgtgtcggt tcgagtccga ccctgggcac
     7141 cacaaccgct tttagagcgg ttattttttt gtctattgct gtttaatgaa atagataaga
     7201 c
//
LOCUS       DAWKOD010000097         7099 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000097 DAWKOD010000000
VERSION     DAWKOD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.097"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..428)
                     /gene="rnpB"
                     /locus_tag="WJK73_001471"
     ncRNA           complement(69..428)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WJK73_001471"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJK73_001472"
     CDS             complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJK73_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ7557944.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1713..2606)
                     /locus_tag="WJK73_001473"
     CDS             complement(1713..2606)
                     /locus_tag="WJK73_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ7557945.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2948..3397
                     /locus_tag="WJK73_001474"
     CDS             2948..3397
                     /locus_tag="WJK73_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7557946.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3471..3779
                     /locus_tag="WJK73_001475"
     CDS             3471..3779
                     /locus_tag="WJK73_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557947.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WJK73_001476"
     CDS             3895..4737
                     /locus_tag="WJK73_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ7557948.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4727..5473
                     /locus_tag="WJK73_001477"
     CDS             4727..5473
                     /locus_tag="WJK73_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ7557949.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WJK73_001478"
     CDS             5495..6082
                     /locus_tag="WJK73_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ7557950.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WJK73_001479"
     CDS             6088..6267
                     /locus_tag="WJK73_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ7557951.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6508..6948
                     /locus_tag="WJK73_001480"
     CDS             6508..6948
                     /locus_tag="WJK73_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557952.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1793 a         1890 c         1628 g         1788 t
ORIGIN      
        1 acacaaccta aataaaaatg ccgtctgaac catatttcag gtttcagacg gcatttgcct
       61 gtcggatgca caccggacag gcggtaagcc gggttctgtc tcggacagtc attcctctag
      121 gcataccgtt gccggtatgc tcaagcaacc tacccgaacg ctcggcgggc agcgtcattg
      181 cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt accaaatgcg
      241 cggtgcgccc ttaccgcacc ttttcaccct tgcctgtgct gccaaagcag ccatcggcgg
      301 ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac cggcattcta
      361 ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc gactgtctgc
      421 ccgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct gaaacggtac
      481 aggtttcaga cggcatacag cctaaactac acgccctgtt tcaggctggc ttcgataaag
      541 ccgtccaagt cgccgtccaa tacggctttg gtgttgccga cttcgtagcc tgtgcgcaag
      601 tctttaatgc gtgaggagtc caagacgtac gaacggattt ggctgcccca acctacatcg
      661 gatttacctt cttccaacgc ctgtttctct tcgttgcgtt tgcgcatttc caattcatac
      721 agtttggact tcaacatttc catcgcggcg gctttgttgg cgtgttgcga acggtcgttt
      781 tgacattgca ccacaatccc cgtcggctcg tgggtaatac gcacggcgga gtcggttttg
      841 ttgatgtgct gaccgcccgc gcccgatgcg cgataggtgt cgatacgcaa atcggcgggg
      901 ttgatttcga tttcaatgga atcatcgatt tcagggtaaa cgaacacgga ggcaaacgaa
      961 gtatggcgtt tgttgttcga gtcaaacggt gaatagcgca ccaagcggtg aacgccggtt
     1021 tcggtacgca gcaaaccata ggcgtattcg ccttccactc ggatagtcgc gcggttgatg
     1081 cccgcgattt cgccgtcgtc ttcttcgagg atttcgattt tgaagccttt gcgctcggcg
     1141 tagcggctgt acatacggaa cagcataccc gcccagtctt ccgcttccgt accgcccgcg
     1201 cctgcggtga tgtcgataaa gcagttgttc gggtcggcgg gctggttgaa catccgtttg
     1261 aactccaaat ccgccatctg tttttccagc cccgccacat cttcctgcac ggcggcaaaa
     1321 ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc ttcgatgccc
     1381 gtcgcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc tttgccgatt
     1441 tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc gataacttct
     1501 tccaatcggt ctttcttacc ctgataatcc atataaactc ggatgtcttc gctgcgcttt
     1561 tccaaatcat tcagggtatt gttgagctgg ttgattactt cggcttccat gattcttttg
     1621 ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc tatggataaa
     1681 gccttctgga aacacgttcg gacggcatag cgtcaataac ggtatgccgc cagtttgcgt
     1741 ttgatttcag gcaatgcggc acgtgctgcc tcctcaccca accggatggc gcgttttttc
     1801 tgatcgaatc cgccgactgc acccaaatcc aaaacctgcg gtttgataac cacatccgcc
     1861 tgccccaact cattttgcaa cgcggaaacg ctcattacgt tcagcgtctg atcgagataa
     1921 gagaagaagc cttggctgat gtttttgctc ggacgggcgg aaatatcgac ggcaatcacg
     1981 aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag accgccgtca
     2041 acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc ggcggaagcg
     2101 cgcacagcct gcccggcatt cccttgattg aaagcgacgg ccttgccggt ttcaaaatca
     2161 gtagcaacgg cggcaaattt gatgggaaac tgctgaatcc gcctgccgcc gacttttcgg
     2221 ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa ggttaaatcg
     2281 accaaatcgg ttttacctaa aatttcggct tccaattcga ggcggtcggg cgacataccc
     2341 gatgcaaaaa ggctgccgac tatcgaacct gccgatgtgc cggtaaccac cttcacagga
     2401 ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt agatgcgccg
     2461 ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccgg cttgcgggca
     2521 gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat tgccaaaagc
     2581 ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcgc accccgtcaa
     2641 aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg gggctttgtt
     2701 aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat ttgaaggcat
     2761 actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc cgcctttttc
     2821 ccttcctttc catcaaaata ctttcttttt atattcatta acttgttaaa tcattggctg
     2881 ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa ccaaaacagg
     2941 aacacttatg aaaatcggaa caacttggca gacggcatcc gctatgctgg ttttgcgtct
     3001 gtttgccgca tatgaatttt tggaatcggg tttgcaaaaa tggaacgggg agaattggtt
     3061 ttccgaaatc aacgatcagt ttccattccc gttcaacttg ctgccggacg cgttaaactg
     3121 gaatctcgcc atgtatgcgg agcttttact gcccgtactg ttgcttttgg gtttggcaac
     3181 gcgtctgtcg gcattagggc tgatggtcgt taccgccgtc gcttgggctg cggttcacgc
     3241 cggttcgggt tacaatgtct gcgacaacgg ttataaaatg gctttaattt atatcgtggt
     3301 attaatcccg ctgcttttcc agggtgcggg cggatggtcg ctggatacgc tgctgaaaaa
     3361 acggttttgc ccccaatgcc gtctgaagca agattgattc agtcgtggaa tctgacttta
     3421 aacattccaa ccttatctcg ttaacttgat attctgaaaa ggaaatagaa atgaacaaaa
     3481 acattgctgc cgcactcgcc ggtgctttat ccctgtcttt ggccgccggt gcagttgctg
     3541 ccaacaaacc agcaagcaac gcaacaggcg ttcataaatc cgcccatggc tcttgcggcg
     3601 cgtccaaatc tgccgaaggt tcgtgcggcg cggctggttc taaagcaggc gaaggcaaat
     3661 gcggcgaggg caaatgcggt gcgaccgtaa aaaaaaccca caaacacacc aaagcatcta
     3721 aagccaaggc caaatctgcc gaaggcaaat gcggcgaagg caaatgcggt tctaaataat
     3781 cccacccctt caaaccaagc cgcgtttttc agtaaaatgc ggctttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcacgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattgtct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt accgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc ggttgcacgt
     4381 gaggccgatt gcggcattca tctggacgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaagctgcc gacgattccg
     4621 cccaccctat tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaggtc
     4681 gccaaaattg ccgactatca aacgcgtgcc ggaaaggaat accgccgtgc agcctgaaat
     4741 ctccgcccaa taccaacacc gtttcgccca agccatacgc gagggtgaag ccgccgacgg
     4801 actgccgcaa gagcgcctga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gcttttagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccctc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctgg agataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccgcac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaac aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgctata tttgctttat ttaccttatt tcacagacgg cgctacccct cccgcccaac
     6481 ccgttctttc tgaatgagca gatttcaatg attaaggaaa ccctaatgcg cccaatcttc
     6541 ctatctttcg ttttattccc tattttgata accgcctgca gcacaccgga caagtctgcc
     6601 cgatgggaaa atatcggcac aatctcaaac ggcaatattc atacatatat caataaagac
     6661 agcgtgagaa aaaacggaaa tctgatgatt ttccaagata aaaaagttgt taccaatcta
     6721 aaacaagaac gttttgccaa cacccccgca tacaagactg ccattgccga gtgggaaatc
     6781 cactgcaaca acaaaacata ccgcttaagt tcgctacaat tgtttgatac aaaaaacacg
     6841 gaaatttcca cacaaaacta cacagcctct tccctccgcc cgatgagcat cctgtccggg
     6901 acattaaccg aaaaacaata tgaaaccgta tgcggaaaaa aactctgatt gcaacttata
     6961 cacaaactta cccacaaacc ttatcataaa aatgccgtct gaaatactga aatatcagca
     7021 tttcggacgg cattttgcca tttctccgaa aagttatcct cgaagttatc cacattattt
     7081 ttttaaaccg gcttccatc
//
LOCUS       DAWKOD010000098         7074 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000098 DAWKOD010000000
VERSION     DAWKOD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.098"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            121..1971
                     /locus_tag="WJK73_001481"
     CDS             121..1971
                     /locus_tag="WJK73_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7557953.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2260..2472
                     /locus_tag="WJK73_001482"
     CDS             2260..2472
                     /locus_tag="WJK73_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ7557954.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2604..3050
                     /locus_tag="WJK73_001483"
     CDS             2604..3050
                     /locus_tag="WJK73_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ7557955.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3128..3370)
                     /locus_tag="WJK73_001484"
     CDS             complement(3128..3370)
                     /locus_tag="WJK73_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557956.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3375..3767)
                     /locus_tag="WJK73_001485"
     CDS             complement(3375..3767)
                     /locus_tag="WJK73_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ7557957.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3839..4444)
                     /locus_tag="WJK73_001486"
     CDS             complement(3839..4444)
                     /locus_tag="WJK73_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7557958.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4546..6222)
                     /locus_tag="WJK73_001487"
     CDS             complement(4546..6222)
                     /locus_tag="WJK73_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ7557959.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6368..6997)
                     /locus_tag="WJK73_001488"
     CDS             complement(6368..6997)
                     /locus_tag="WJK73_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ7557960.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         1833 a         1949 c         1892 g         1400 t
ORIGIN      
        1 gtcggaaaca ttccaaaggc ggtgcagttt cggcatataa ttcgggcaaa cgcctgttca
       61 gacggcattt tgtcttttcc aaccctgacc gttcagggtt ccgattctta aggaaatccg
      121 atgtacctac cctctatgaa gcattccctg ccgctgctgg cgaccctagt gcttgccgcg
      181 tgttcttcga caaacacact gccagccggc aagaccccgg cagacaatat agaaactgcc
      241 gacctttcgg caagcgttcc cacccgccct gccgaaccgg aaggaaaaac gctggcagat
      301 tacggcggct acccgtccgc actggatgca gtgaaacaga aaaacgatgc cgccgtcgcc
      361 gcctatttgg aaaacgccgg cgacagtgcg atggcggaaa atgtccgcaa cgagtggctg
      421 aagtctttgg gcgcacgcaa acagtggacg ctgtttgcac aggaatatgc caaactcgaa
      481 ccggcagggc gcgcccaaga agtcgaatgc tacgccgatt cgagccgcaa cgactatacg
      541 cgtgccgccg aactggtcaa aaatacgggc aaactgcctt cgggctgcac caaactgttg
      601 gaacaggcag ccgcatccgg cttgttggac ggcaacgacg cctggaggcg cgtgcgcgga
      661 ctgctggccg gccgccaaac cacagacgca cgcaaccttg ccgccgcatt gggcagcccg
      721 tttgacggcg gtacacaagg ttcgcgcgaa tatgccctgt tgaacgtcat cggcaaagaa
      781 gcacgcaaat cgccgaatgc cgccgccctg ctgtccgaaa tggaaagcgg tttaagcctc
      841 gaacaacgca gtttcgcgtg gggcgtattg gggcattatc agtcgcaaaa cctcaatgtg
      901 cctgccgcct tggactatta cggcaaggtt gccgaccgcc gccaactgac cgacgaccaa
      961 atcgagtggt acgcccgcgc cgccttgcgc gcccgacgtt gggacgagct ggcctccgtt
     1021 atctcgcata tgcccgaaaa actgcaaaaa agcccgacct ggctctactg gctggcacgc
     1081 agccgcgccg caacgggcaa cacgcaagag gcggaaaaac tttacaaaca ggcggcagcg
     1141 acgggcagga atttttatgc ggtgctggca ggggaagagt tggggcgcaa aatcgatacg
     1201 cgcaacaatg ttcccgatgc cgacgcaaac agcgtcctcc gtatggcgga agacggcgcg
     1261 attaagcgtg cgctggtact gttccaaaac agccggaccg ccggcgatgc gaaaatgcgc
     1321 cgtcaggctc aggcggaatg gcgttttgcc acacgcgggt tcgacgaaga caagctgctg
     1381 accgccgcac aaaccgcgtt cgaccacggt ttttacgata tggcggtcaa cagcgcggaa
     1441 cgcaccgacc gcaaactcaa ctacaccttg cgctatattt cgccgtttaa agacacggta
     1501 atccgccacg cgcaaaatgt taatgtcgat ccggcttggg tttacggcct gattcgtcag
     1561 gaaagccgct tcgttatagg cgcgcaatcc cgcgtaggcg cgcaggggct gatgcaggtt
     1621 atgcctgcca ccgcgcgcga aatcgccggc aaaatcggta tggatgccgc acaactttac
     1681 accgccgacg gcaatatccg tatggggacg tggtatatgg cggacaccaa acgccgcctg
     1741 caaaacaacg aagtcctcgc caccgcaggc tataacgccg gtcccggcag ggcgcgccga
     1801 tggcaggcgg acacgcccct cgaaggcgcg gtatatgccg aaaccatccc gttttccgaa
     1861 acgcgcgact atgtcaaaaa agtgatggcc aatgccgcct actacgcctc cctcttcggc
     1921 gcgccgcaca tcccgctcaa acagcgtatg ggcattgtcc ccgcccgctg acgtaccgat
     1981 gccgtctgaa acccgcccgg tctttcagac ggcattttta tcccgaacgg cattgacggc
     2041 gaaccataaa tataagacaa tccgaaaatt gtttttcctg ctttttcaag cagcttgaca
     2101 cggcacaagc cgacccgtta ggaggtgatg tttccgtcac ggcgcgtatc ccgccgccgc
     2161 aaggcacagc gatacggtaa accttcaaca ccgtctgccc taccctttcc accgatatga
     2221 tgggcagatg aaacaaccga atttattaaa ggaaataaaa tgcctgcaat ccgcgtaaaa
     2281 gagaatgaac catttgaagt cgctatgcgc cgtttcaaac gcgccgtaga aaaaaccggc
     2341 ctgctgaccg agctgcgcgc ccgcgaagcc tacgaaaaac cgactaccga acgcaaacgc
     2401 aaaaaagcgg cagccgtaaa acgcctgcaa aaacgcctgc gcagccaaca actgccgccc
     2461 aaaatgtact aaacgttcaa gtacagatta caggtcagcc ctgtgatatg aggacacacc
     2521 gcaagacctg ctctgcggtg tgttttgctt ttcagacggc atcgaaaccc gccgtttcca
     2581 tccgacatcc cagcgaggac atcatgagcc tgaaaatccg ccttaccgaa gacatgaaaa
     2641 ccgcgatgcg cgccaaagac caagtttccc tcggcaccat ccgcctcatc aacgccgccg
     2701 tcaaacagtt tgaagtggac gaacgcaccg aagccgacga tgccaaaatc accgccatcc
     2761 tgaccaaaat ggtcaaacag cgaaaagaca gcgcgaaaat ctacactgaa gccggccgtc
     2821 aggatttggc agacaaagaa aacgccgaaa tcgaggtact gcaccgctac cttccccaaa
     2881 tgctctccgc cggggaaatc cgtactgcgg tcgatgccgc cgttgccgaa accggcgcgg
     2941 caggtatggc ggatatgggc aaagtgatgg gcgtattgaa aacccgcctc gccggcaaag
     3001 ccgatatggg cgaagtcaac aaaatcctga aagccgtgct gaccgcctga ttgcccgaat
     3061 atcggacaaa atgccgtctg aagcccgtat cgcaggttca gacggcattt tcatggtttg
     3121 atgatattta taaaaatttt gcaacacgct catgcacagc aactgccgct gctcgttggc
     3181 gttgccgctc tatcgcttgg ttagctgcac cataattgca ctctaatgct gatgcaataa
     3241 cgtcccaata tcgaatcggc aggggcaaca cggtttcgat acaccgaaac gggttttgcc
     3301 gataaacgga ttccgtttgc gccccatcgg acaaaatgcc gtctgaaaca cgattccgtt
     3361 cagacggcat agatttattt gaccaatttc aagccttttt tggcgggccg gggcgcggtt
     3421 tcggcagagg tgttttcagg cggcgtgtcg gggcgatacg cttccaattc aaaccccata
     3481 ccttcgccgg tctcccgtgc gaaaaggctg aggacgtgtc cgacaggtat ccatatgtcg
     3541 tgcgcctgtc cgccgaagcg ggcggaaaag ctgatccaat cgttgtcgat ttgaaggttt
     3601 tgcgtggcgg tcgcgccgat gttgagcata atttcgttgt cgcggacgta ctgcatgggg
     3661 acacgcgtgt gttcgttgac ccagacaaga atgtgcggcg tgaggctgtt atcgctgcac
     3721 cattcgcaaa gtgcgcggag gatgtagggt ttggtggaag tgggcataat gggttccgtg
     3781 tggtgtgcct gaatgtgaaa atgcctacaa aacggcgggt tttgcaagca ttccgggctt
     3841 atttgcgcat ggctttttcg gcgggtgtca gtgcttcgat aaaggcttcg cgctggaaga
     3901 tgcgctcggc gtatttgagc agcggcgcgg cacttttgcc cagtttgaca tcgtagtggt
     3961 cgagccgcca cagcagcgga gcaagggcga catcaatcat agaaaaatct tcgccgagga
     4021 tgtatttgct tttgctgaac gaaggggcaa gcatggtcag accgttgccg atggcttcgc
     4081 gcgcttttgc ctgttccttg ttggtggcgg cggggttttc taacacttgg acgtggttga
     4141 acaattcttt ttccatacgg tacagcacca gccggccccg gccgcgcata acgggatcgc
     4201 cgggcataag ctgcggatga gggaagcgtt cgtcgatgta ttcgttgatg atgttggact
     4261 cgtgcagcac caaatcgcgc tcgaccagca cgggaacttg gttatacgga ttcatgacgg
     4321 cgaggtcttc gggtttgttg taaatatcga cgtctttgat ttcaaaatcc ataccttttt
     4381 cgtacaaaac gaagcggcag cggtggctga aggggcaggt aatgccggaa tagagggtca
     4441 tcataataat tgtcgctcct gtgtgatgcc tgcaaaacgg ctgatttata aaggtttaat
     4501 cgcgcaatta tacgcgattt ccggcactta atccagaaat tcggctcaat ctgttgtttt
     4561 taatatattt tccccgattt tccgtatcag tgcgaactta ctgtctttgt tgcgcgggcg
     4621 cgcaccctgc cagaccgtct gccagccttg cgacgcatcc gcattttggg gcaagcggac
     4681 gatacggtag cggcattgta catcgccgac gcggtgcggc aatgtgccgt actgcgtcca
     4741 aacaatccgc gtgtgcaggt cgccgccgcc tatgtcgata cactcgatgc cgtctgaaag
     4801 ctcccgtttt aattccgggg aaagcgatgc ctccatactc cggacgaccg gcgcgtggct
     4861 tttcgccgcg tccagccacg gcaggaacag cgtcatcagc aaagcccagg tcagggtaac
     4921 gcctgccgcc cagttggtaa ccgcctgcct gccgcgtatg tttttccggg taatcgccca
     4981 cagccacaag ggtgtgaaca gtacggcaac cgccatcgga atgggatcga tatcaggaac
     5041 ataatacggg ctgaaatagg cggcgcgttc ggcaagcttg gcgggccagc cgtaattcat
     5101 ggcgaaaaag cccgtccaca ggaacacggc aaacagtccg aacgccataa tgccgaacca
     5161 gttgacaaac gccgccgcgc cgcgcctcag gctgtccagt tgcgccgcgc cgaacagggc
     5221 aagcggcgga agcagccaga cgaggttatc ctgaaaacgc tgcggattga cggcaagcag
     5281 caccaaaacg gcaagcatcc agacgacgcc caaaatcccc cagtcggtcg aaaacaggcg
     5341 cgtgcggcaa accgtccaaa ccgccagcgg cagcgcgggc aatgcaaacc aaagcaggtt
     5401 tttcagatag taaaacaaac tgaatgccgt ctgaacgtgc cgcacgccgc cgaacgtacc
     5461 gaaaacgtga tcgtcgagcc attgcgcgaa cagcgcgggc tgcgtttttg ccaagagcag
     5521 cgggtaaacg gtcataagcg gcagggcaaa ggcaagtgag gcgactgccg tcaacatcaa
     5581 acgcctgctt tgccacggac ggaaaaacat cagtacgggc aagggcagca tcagggcaaa
     5641 tgctgccgga taagctgctg ccaacgacat cagcgtccag cccgtaccga gcagaaaaga
     5701 ggcggcaatc acgcgccggc gagccaaaga ataaccgtgc agcaccagtc cggcggcggc
     5761 aaaggcggcg gcagcggggt tgagaaagtg tacggtcgga atcagcccga tacagccgat
     5821 gagaatcagg acgacgctgc gcccgtggtg tctgcccaaa aagttgaagc cagcaaagcc
     5881 gcaggaagtc agcccgacaa cggcgaaaaa cacgcctgca aagcgtgcgg catcatacgg
     5941 gtcggctgcc cacggcgaca gcaaatgttt gaacgcggcg gcaacccaaa gatacacggg
     6001 tggtatgccg aaatcggcat gcccgaacag atgggcaacc aagggggtgg ggctgcctgc
     6061 cagtgcttcg acggcggtat agacggcagg ttcggcagga ttccacaaat cgtgggaaaa
     6121 cacgccgggc cacaaccagg caaacgccat caacagcagc agccacggct tttcgtgggt
     6181 tttggcgggc gggcgggcat cgggcggagt gtaggtcagc ataagcgtga gaaaaaatgg
     6241 acggattgcc aagtgtagca aatattcgca caaaggtcgt gcagagactg cttcagacgg
     6301 catcagacac aaaaagaccg gcaacaaaaa agactgcaca tggcagtctt tgcagatact
     6361 atctttttca taatattttt tcctggcccg acacagtcca tagcatatca aaactgttgt
     6421 tttcaatcag gatatatacc cccaatccta aataaacaac agcaacaaac catctgctat
     6481 atttttccaa agtttctccg acagaaggga cttgtgccaa tttttgggca gaaaaaacca
     6541 agagataaat catgactaga aaggtaagta aagccactat caaattcgct gaatttaagg
     6601 tagtaaaata tgggacaaag acaccaatat tgtcagcacc acaacttgca aaagtaatca
     6661 tagcgactag aaaaatcagg tttttattat ctttgcgcaa accctctttg gcaatagcct
     6721 ctccatcaga atctcctaac agcaaaactt tgatgcctag gaaaattgga atcaagccga
     6781 gcaaacctaa aatctcttta ctaggaatat aatctaagac aaatgcaaaa agcaaactta
     6841 gcaatatcag actaacagag cctagaaatt gtcctaaata gatgttaatg atgtcttttc
     6901 tgctttttct tttggcaaaa aataacatta ggataataag taagtctacg gctgtcccag
     6961 aatacaggat tattgaagta acgacatttt gaatcataaa acatctcatt caaatatatt
     7021 tttaaatgta ttcaaacatt aaaccttgta gatgtcaact tcaaccccgt caaa
//
LOCUS       DAWKOD010000099         7061 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000099 DAWKOD010000000
VERSION     DAWKOD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.099"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            11..313
                     /locus_tag="WJK73_001489"
     CDS             11..313
                     /locus_tag="WJK73_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557961.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            593..751
                     /locus_tag="WJK73_001490"
                     /pseudo
     CDS             593..751
                     /locus_tag="WJK73_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            896..2638
                     /gene="hrpB"
                     /locus_tag="WJK73_001491"
     CDS             896..2638
                     /gene="hrpB"
                     /locus_tag="WJK73_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ7557962.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            2731..>7061
                     /locus_tag="WJK73_001492"
     CDS             2731..>7061
                     /locus_tag="WJK73_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7557963.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLETSNVASNIRLNNGSIKGG
                     KQVVLMADDDIQAKASHLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSN
                     GNTLTAEKNLDIQAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAAL
                     QGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIY
                     ASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTI
                     NNSKLNSGHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSK
                     STLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGHIDWETQDTATMRSAEL
                     KPLSGMMSIESGGNNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASV
                     SAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQ
                     QLTDVEQEFSKLTSDPKYRKAQDLSQMLRRKYKRRDKVFGDSEARLRGLRAKINAADE
                     AWAERQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGS
                     KVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFS
                     RPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKIQSYGDQYYYARQSELYTF
                     ERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQGIDIKSGGNIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAE
                     SANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSK
                     STLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIPKGNLKTQIETLTKQPEY
                     AYLKQLQV"
BASE COUNT         2092 a         1655 c         1676 g         1638 t
ORIGIN      
        1 tgttcatgaa atgacttggg tcggaggctc aggtaatgcg caacaaagct catattattg
       61 cgaaatttgc gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt
      121 gccaaacaat acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg
      181 tagccgcata gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga
      241 ctggtcatgg tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg
      301 cggtttgcct taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca
      361 ctcggcatca tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg
      421 acgtattgga tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta
      481 gagaaaagct ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg
      541 cggcctttct aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc
      601 cccatctgca aaacctgtct ttgggcttaa aaaaaagctg cctgttatcc tgcaaacaga
      661 aatatcagaa tgcggcttgg catgtctggc ggctgtggcg ggatttcatg gtttccatac
      721 ggatttacgc gcactgcgtt caaaatactg tccgagacct ttgcaaaatt ccccaaaatc
      781 ccctaaatgt cttggtggga attttgggga attttgcaaa ggtctcattc tataactgta
      841 aatactttta aatttatgac aaaatagtaa atattgctaa aataatattg atgtcatgaa
      901 attttttcct gctccatgtc tgttggttat cctggctgtc atacccctta aaaccttagc
      961 tgccgatgaa aacgatgcag aacttatccg ttccatgcag cgtcagcagc acatagatgc
     1021 tgaattgtta actgatgcaa atgtccgttt cgagcaacca ttggagaaga acaattatgt
     1081 cctgagtgaa gatgaaacac cgtgtactcg ggtaaattac attagtttag atgataagac
     1141 ggtgcgcaaa ttttcttttc ttccttctgt gctcatgaaa gaaacagctt ttaaaactgg
     1201 gatgtgttta ggttccaata atttgagcag gctacaaaaa gccgcgcaac agatactgat
     1261 cgtgcgtggc tacctcactt cccaagctat tatccaacca cagaatatgg attcgggaat
     1321 tctgaaatta cgggtatcag caggcgaaat aggggatatc cgctatgaag aaaaacggga
     1381 tgggaagtct gccgagggca gtattagtgc attcaataac aaatttccct tatataggaa
     1441 caaaattctc aatcttcgcg atgtagagca gggcttggaa aacctgcgtc gtttgtcgag
     1501 tgttaaaaca gatattcaga ttataccgtc cgaagaagaa ggcaaaagcg atttacagat
     1561 caaatggcag cagaataaac ccatacggtt cagtatcggt atagatgatg cgggcggcaa
     1621 aacgaccggc aaatatcaag gaaatgtcgc tttatcgttc gataaccctt tgggcttaag
     1681 cgatttgttt tatgtttcat atggacgcgg tttggcgcac aaaacggact tgactgatgc
     1741 caccggtacg gaaactgcaa gcggatccag aagttacagc gtgcattatt cggtgcccgt
     1801 aaaaaaatgg ctgttttctt ttaatcacaa tggacatcgt taccacgaag caaccgaagg
     1861 ctattccgtc aattacgatt acaacggcaa acaatatcag agcagcttgg ccgccgagcg
     1921 catgctttgg cgtaacaggt ttcataaaac ttcagtcgga atgaaattat ggacacgcca
     1981 aacctataaa tacatcgacg atgccgaaat cgaagtgcaa cgccgccgct ctgcaggctg
     2041 ggaagccgaa ttgcgccacc gtgcttacct caaccgctgg caactggacg gcaagttatc
     2101 ttacaaacgg ggtaccggta tgcgccaaag tatgcccgca cctgaagaaa acggcggcgg
     2161 tactattcca ggcacatccc gtatgaaaat cataaccgcc ggattggatg cagcggcccc
     2221 gtttatgttg ggcaaacagc agttttccta cgaaaccgct atccatgcac aatggaacaa
     2281 aacgcctttg gttgcccaag acaagttgtc tatcggcagc cgctacaccg ttcgaggatt
     2341 tgacggtgaa cagagccttt ccggagagcg aggtttctac tggcagaaca ctttgggctg
     2401 gcatttccat ccgcgccatc aattctatct cggcgcggac tatggtcggg tatcgggcga
     2461 aagtgcccaa tatgcctcgg gcaagcggct ggcgggtgca gtggccggct tcagaggcag
     2521 ccataaagta ggcggtatat ttgcttatga cttatttgcc ggcaagccgc tgcacaagcc
     2581 cgaaggtttt cagacggcca acaccgttta cggcttcaat ttgaattaca gtttctaacc
     2641 ctctcctgca ttttttaccg acattcagac agtctttcat catttacagg ccgtctgaag
     2701 cgattacaaa catcatttaa agggataaat atgaacaaag gtctgcatcg taccgttttc
     2761 agcaaaaagc acaacactat ggttgcagtt gccgaaaccg ccaacagtca gggaaaaggc
     2821 aaacaggcag gaacttccgc tccggcttcg ccacaaattt cagacggcct ttacagtaaa
     2881 ctcaaaacca cgcttaaaac cttggtctgc tctttggttt ccgtcagtat ggtattgcct
     2941 gcccacgcac aaatcattac cgataaaacc gcgcccaaaa accagcaggc cgttattctt
     3001 aaaaccaata ccggtgcgcc gttggtcaac atccaaactc ctaatgcccg tggattgagc
     3061 cacaaccgct atacccagtt tgatgtcgac gctaaaggtg ctgtactgaa caacgatcgc
     3121 aacaacaacc cgtttctggc caaaggcagt gcgcagttga ttttgaacga ggtgcgcggt
     3181 gcggccagca aactcaatgg catcgttacc gtaggtggtc aaaaggccga tgtgattatc
     3241 gccaacccca acggcattac cgtgaacggc ggcggcttta aaaatgtcgg ccgcggtatc
     3301 ttaactaccg gtgcgcccca aatcggcaaa gacggtgcac tgacaggatt tgatgtgcgt
     3361 caaggcacat tgaccgtagg agcagcaggt tggaatgata aaggcggagc cgactacacc
     3421 gagatacttg ctcgtgcagt tgctttgcag gggaaattac agggtaaaaa cctggcggtt
     3481 tctaccggtc ctcagaaagt agattacgcc agcggcgaaa tcagtgcagg tacggcagcg
     3541 ggtacgaaac cgactattgc ccttgatact gccgcactgg gcggtatgta cgcagacagc
     3601 atcacactga ttgccaatga aaaaggcgta ggcgtcaaaa atgccggcac actcgaagcg
     3661 gccaagcaat tgattgtgac ttcgtcaggc cgcattgaaa acagcggccg catcgccacc
     3721 actgccgacg gcaccgaagc ttcaccgact tatctctcca tcgaaaccac cgaaaaagga
     3781 gcggcaggcg catttatctc caatggcggc cggatagaga gcaaaggctt attggttatt
     3841 gataccgggg aggacatcgg tttgcgtaac ggagcggtcg ttcaaaacaa cggcaaccgc
     3901 cctgctgcta cggtattaaa tgccggccgt aatttggtta tcgaaagcaa agcaaatgtg
     3961 aacaatgcta aaggttcgac caatctgtcg gccggcggtc gtactacgat caatgatgct
     4021 actattcaag cgggcagttc cgtgtacagc tccaccaaag gcgatactga attgggtgaa
     4081 aatacccgta ttattgctga aaacgtaacc gtattgtcca acggcagtat tggcagtgct
     4141 gctgtaattg aggctaaaga cactgcacac attgaagcgg gtaaaccgct ttctttagag
     4201 acttcaaacg ttgcttctaa catccgtttg aacaacggta gcattaaagg cggaaagcag
     4261 gtggtattga tggctgatga cgacattcag gcaaaagcct cccacctgaa tgcgtccggc
     4321 aatctgtata ttcatgcagg taaggatctg gatttgaatg ccgataaaga cttatcaaca
     4381 caaagtatca gcctgagggc agacaacacc gccctcatca gcagcaacgg taacaccttg
     4441 actgcagaaa agaatctgga tattcaggca ggtagcttga gtgtgcgtca aagtaatctg
     4501 caatccagtg gcggtaatgt gcagatgagc gcgactaaag gcaacatcag cttgaatcag
     4561 agttggataa atgccagtca gaatattgat acggcagcac ttcaaggcaa tattatttca
     4621 gacggtctga ctgctgttgc cgaagttgga cgcgtatctc ttcttgccaa cggcaatgtt
     4681 gatttcaacg gtctaaatac cttgattgcg gaaggagaca ttaatgctgg ttcagtcggt
     4741 aaaggccgtc tgaaaatgga caataccgat atttatgctt ctgcaggcga tgtgaaactg
     4801 gttgccggag gtcaattaga tttaggcaac ggcaccgtta acggcggtca tatcagctta
     4861 gacagcaata aaggcagcat ggtggtgcag gatgtacacc tgaatgcccg cgcatcactg
     4921 aaagtggatg cggatcaaac tttaaccatt aataacagca agctcaattc cggtcacaat
     4981 acccagatta atacaaatca tggtcatatg acgcttaatc agcttgatgc tcattcacgt
     5041 cgtcatatga gtatcagtgc acagggtaaa ggcaaaggaa aagacagcgg tcaaatttta
     5101 caaaacgacc agcaaaacag taaaagtact ttggcggcag atggtgtatt gtcattgaac
     5161 agcagcgcat tacaggtttt ggacaatact accctgcgcg gtggcgcgat aaacatcaaa
     5221 gctggcggag gcatcatcaa acgggggcat atcgattggg aaacccaaga tacggcaact
     5281 atgcgttcag cggaactgaa accgctgtcc ggtatgatgt cgatagaatc gggcggtaat
     5341 aaccctttaa ccgttgaacc cggtaaccgt atcgtttctg caggtgattt ggccgtgaac
     5401 cacaacggca cattccaaat cagtgccaga gcaggaaata acggcaatcc gagtgcgcaa
     5461 acagccagcg tttcagctaa aggcaatatc gggattgtgg cgggagaggt ggatattgat
     5521 gcagcaaata ttgccgcagg gaaagatttg gctttagtgg caactaaagg gaatatttca
     5581 ctaaacagta ttaggaatac gtttagtaac tatcaattga aaacagataa acacaatatc
     5641 acacagcaac ttactgatgt agagcaggag tttagtaagt taacgagcga tcctaaatat
     5701 cgtaaagcac aggatctttc acaaatgttg aggcgtaaat ataagaggcg tgacaaagta
     5761 ttcggtgata gtgaggccag attacgtggt ttacgagcaa agataaatgc tgctgatgag
     5821 gcttgggcag aacgccaatc cccagtaaaa gcgttacttg aacgtaaaca attgttacaa
     5881 caagcccttt taacagtttc ccaaccgggt agtggtcacg aaaaccaagg tagcaccctt
     5941 agcggtcaaa atatcaaact gctcgctgca ggtggtatcc gtatccaggg ctccaaagtt
     6001 gctgccaccc aacaggcaaa tatccaagct gcaggatttt tacctgcacc agccgcagag
     6061 gaactgcaag aaggtcgact tcaatcagcc attgacatca gcggtgtatt tgatactttt
     6121 gaatatggtc agcaaggcag cgataaatac ggctatgcca ttttcagcag gccatctgaa
     6181 atttcaggaa aaacaggtgt taccctctct gcaccaaatg ccaatgagaa cagccgcatc
     6241 agtctgagtg cggcaaacat cgaagctgaa aacggcaaaa tcaaaattca atcttacggt
     6301 gaccaatatt actatgcgag acagagcgaa ctctatacct ttgaacgccg cagctacaaa
     6361 accggcaaat ggtacaaccg caaacacatt accgaagtca aagaacacaa aaacgccaag
     6421 cccgacgcag taaccctcag cgcatcccaa ggcatcgaca tcaaatctgg tggcaacatc
     6481 gacgcctacg ccaccgcatt cgatgccccc aaaggcagca ttaacatcga agccgggcgg
     6541 aaattgacac tctatgccgt agaagagctc aactacgaca aactagacag ccaaaaaagg
     6601 cgcagatttc tcggcatcag ctacagcaaa gcacacgaca ccaccaccca agtcatgaaa
     6661 accgcgctgc cctcaagggt agttgcagaa tcagccaacc tccaatcggg ctgggatacc
     6721 aaactgcaag gcacacagtt tgaaaccaca ctgggtggcg caaccatacg cgcaggcgta
     6781 ggcgagcagg cacgggcaga tgccaagatt atcctcgaag ggatcaaaag cagcatccac
     6841 acagaaaccg tgagcagcag caaatctact ctatggcaaa aacaggcagg acggggcagt
     6901 aacatcgaaa ccttgcaatt gccgagtttc accggtcccg ttgcgcccgt actgtccgca
     6961 cccggcggtt acattgtcga tattcccaaa ggcaatctga aaacccaaat cgaaaccctc
     7021 accaagcagc ccgagtatgc ttatttgaaa caacttcaag t
//
LOCUS       DAWKOD010000100         7056 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000100 DAWKOD010000000
VERSION     DAWKOD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.100"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            144..725
                     /locus_tag="WJK73_001493"
     CDS             144..725
                     /locus_tag="WJK73_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7557964.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(787..1641)
                     /gene="folD"
                     /locus_tag="WJK73_001494"
     CDS             complement(787..1641)
                     /gene="folD"
                     /locus_tag="WJK73_001494"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ7557965.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1864..1941
                     /locus_tag="WJK73_001495"
     tRNA            1864..1941
                     /locus_tag="WJK73_001495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Pro,seq:tgg)
     gene            1966..2042
                     /locus_tag="WJK73_001496"
     tRNA            1966..2042
                     /locus_tag="WJK73_001496"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2000..2002,aa:Arg,seq:tct)
     gene            2070..2145
                     /locus_tag="WJK73_001497"
     tRNA            2070..2145
                     /locus_tag="WJK73_001497"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:His,seq:gtg)
     gene            2280..2786
                     /locus_tag="WJK73_001498"
     CDS             2280..2786
                     /locus_tag="WJK73_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ7557966.1"
                     /translation="MVDAWSVPDFESKICPPESLAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2783..4561
                     /locus_tag="WJK73_001499"
     CDS             2783..4561
                     /locus_tag="WJK73_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ7557967.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4674..5498
                     /locus_tag="WJK73_001500"
     CDS             4674..5498
                     /locus_tag="WJK73_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ7557968.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6260..7048)
                     /locus_tag="WJK73_001501"
     CDS             complement(6260..7048)
                     /locus_tag="WJK73_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ7557969.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1508 a         1827 c         2123 g         1598 t
ORIGIN      
        1 aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt aaagttcttt
       61 atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt
      121 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggcatccg
      181 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
      241 ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt gccggactga
      301 ttgcttttgc catcaagaat gaaggcagga ttggcggcag tgaggaaata ttcgggctgc
      361 ttttgggcat gacttccttt tgggtaggac tggcgggcat tttggtttat accgtcgttc
      421 aaatctacta tatgagtcgg gacgggcagt cattgggtaa gaaaatcatg agaatccgcg
      481 tgttgaaaac cgacggccgc aatcccggtt ttgtcggcac ggttttggta cgcgaaatcg
      541 catggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta ggtgacaacg
      601 gagaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc tttatggtca
      661 aacgcgaccg ccgcacgctt tacgacatac tggcggatac ggttgtcgtc aagctgccca
      721 aataaacgga cacggcaaaa tgccgtctga aagcctttca gacggcattt ttatcttcag
      781 aaccgctcaa gcatcgtgca gtgaagccgc gtgcagggtg ttttccatca atgtggcaat
      841 cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt
      901 ttcaaattcc acgtcgccgc acaggctgcc gtcgtccaaa cggttgatgc ccacatcgat
      961 aacgaccgcg ccgggtttga tccattcgcc tttgacaaag ttcggaatgc ccacgccgac
     1021 caccaaaata tcggctccgg caacctcgtc tgtcagattt tcggttgcgc tgtggcagac
     1081 cgttaccgtt gcgcgcgcca gcagcagttc caaagcctgc gggcggccga cgatattcga
     1141 cgcgccgacc acgaccgctt ttttcccctt cggatcaatg ccgtaagctt ccaaaagcgt
     1201 catcacgccc ttgggcgtac acgggcgcat cagcggcatt ttgaccgcca gcctgccgac
     1261 attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca aaaccgcctg
     1321 gctgtcgagg tgcttcggca ggggcagctg aaccagaata ccgtccactt cggaatcggc
     1381 attcaggcgg tcgaccagtg ccagcagttc ttcctgcgat gttgattcgg gcagttcgta
     1441 agacagtgat ttgatgccgc atttttggca ggcagttttc ttgttgcgga cataaaccgc
     1501 gctggcaggg tcgccgccga ccaaaaccac ggccaggcaa ggcgtgtgca gattgtcctg
     1561 ttggcgttgc gccaccgctt cggcaaccgc ctgcaggcgt ttttgcgaaa cttctttacc
     1621 attgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat
     1681 tatcgcattt tccgtttgtt tttacagcag gatgccgaaa ataaatttgc cctgaaataa
     1741 aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata tttttcatct
     1801 tggtgtcttg aaaataaata ttgacgcatc tttccctgag ttgtatagtt cgcttcttca
     1861 agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg gtcgtgagtt
     1921 cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa
     1981 ccggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc
     2041 caagaatttg attgaaaacc gtttttatgg tggctgtagc tcagttggta gagccccgga
     2101 ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa aggcccgtac
     2161 ttttcggtac gggtcttttc tcgtttgcgc gtactgtccg cgccgccggc tttgcctttc
     2221 gcatatgctt ctgtatcatg taggttttct gtcttttatg gtttttgtcg gaggctttga
     2281 tggttgacgc ttggtctgtc cctgattttg aatcgaaaat ctgtccgccc gagtcgttgg
     2341 cggcgcgttt ggcgttgttg ccgcgcccgc tggtgtttac caacggctgt ttcgacatcc
     2401 tccacagggg gcatgttact tatctggcgc aggcgcgttc gatggggggc gcgttggtgc
     2461 tggcgttgaa taccgatgct tcggtgcggc gtctgggcaa gggcggtgac cgcccggtta
     2521 atcctttgga gaaccgtgcc gccgttgccg ccgcgttgga aagtgtggat ttggtaacgt
     2581 ggtttgacgg ggatacgccg gcggcgttga ttgaggcggt caaacctgaa attttggtca
     2641 agggcggcga ttgggctgcg gataagattg tcggtgcggc agaaacgttg gcgcgcggcg
     2701 gtcaggtgtt ttcaattccg tttctgcacc agacttcgac aacgaagact ttggcaaaaa
     2761 tccgtgcggc agagggcgga aaatgacggt tttgaagcct tcgcactggc gggtgttggc
     2821 ggagcttgcc gacggtttgc cgcaacacgt ctcgcaactg gcgcgtatgg cggatatgaa
     2881 gccgcagcag ctcaacggtt tttggcagca gatgccggcg cacatacgcg ggctgttgcg
     2941 ccaacacgac ggctattggc ggctggtgcg cccattggcg gttttcgatg ccgaaggttt
     3001 gcgcgagctg ggggaaaggt cgggttttca gacggcattg aagcacgagt gcgcgtccag
     3061 caacgacgag atactggaat tggcgcggat tgcgccggac aaggcgcaca aaaccatatg
     3121 tgtgacccac ctgcaaagta agggcagggg gcggcagggg cggaagtggt cgcaccgttt
     3181 gggcgagtgt ctgatgttca gttttggctg ggtgtttgac cggccgcagt atgagttggg
     3241 ttcgctgtcg cctgttgcgg cagtggcgtg ccggcgcgcc ttgtcgcgtt tgggtttgaa
     3301 aacgcaaatc aagtggccaa acgatttggt cgtcggacgc gacaaattgg gcggcattct
     3361 gattgaaacg gtcaggacgg gcggcaaaac ggttgccgtg gtcggtatcg gcatcaattt
     3421 cgtgctgccc aaggaagtgg aaaacgccgc ttccgtgcaa tcgctgtttc agacggcatc
     3481 gcggcgggga aatgccgatg ccgccgtgtt gctggaaacg ctgttggtgg aactggacgc
     3541 ggtgttgttg caatatgcgc gggacggatt tgcgcctttt gtggcggaat atcaggctgc
     3601 caaccgcgac cacggcaagg cggtattgct gttgcgcgac ggcgaaaccg tgttcgaagg
     3661 cacggttaaa ggcgtggacg gacaaggcgt tctgcacttg gaaacggcag agggcaaaca
     3721 gacggtcgtc agcggcgaaa tcagcctgcg gcccgacgac agaccggttt ccgtgccgaa
     3781 gcggcgggat tcggaacgtt ttctgctgtt ggacggcggc aacagccggc tcaagtgggc
     3841 gtgggtggaa aacggcacgt tcgcaaccgt cggtagcgcg ccgtaccgcg atttgtcgcc
     3901 tttgggcgcg gagtgggcgg aaaaggcgga tggaaatgtc cgcatcgtcg gttgcgctgt
     3961 gtgcggagaa ttcaaaaagg cacaagtgca ggaacagctc gcccgaaaaa tcgagtggct
     4021 gccgtcttcg gcacaggctt tgggcatacg caaccactac cgccaccccg aagaacacgg
     4081 ttccgaccgc tggttcaacg ccttgggcag ccgccgcttc agccgcaacg cctgcgtcgt
     4141 cgtcagttgc ggcacggcgg taacggttga cgcgctcacc gatgacggac attatctcgg
     4201 gggaaccatc atgcctggtt tccacctgat gaaagaatcg ctcgccgtcc gaaccgccaa
     4261 cctcaaccgg cacgccggta agcgttatcc tttcccgacc acaacgggca atgccgtcgc
     4321 cagcggcatg atggatgcgg tttgcggctc ggttatgatg atgcacgggc gtttgaaaga
     4381 aaaaaccggg gcgggcaagc ctgtcgatgt catcattacc ggcggcggcg cggcaaaagt
     4441 tgccgaagcc ctgccgcctg catttttggc ggaaaatacc gtgcgcgtgg cggacaacct
     4501 cgtcattcac gggctgctga acctgattgc cgccgaaggc ggggaatcgg aacatactta
     4561 aaaaatacac cctttttcaa cacggggcgg catccgtccg aatccgtccg gattctgccg
     4621 cccgacagac tgccgcccag gcgggcgggg tttcaaacat tgaggaaatt ctaatgaaat
     4681 ggctcttcac ccttttggcg gtactcaata ttgccgtatt cggcggcacg gtgggctata
     4741 aattggccgt taaagcgacg ggcggcgtgc cggaaaaccg ggccgtcgaa aacacgcccc
     4801 ccgcaacgcc cgccgccgac aatgcggcgg catccgtgga ggatacggcg gcactgctca
     4861 aaccgggcga catcctgacc gaagagcagg ccgagcagtt gcgcttgaaa aaggaagcgg
     4921 aacagaaaaa actgagggag aaaaaacagc gtgaagaaaa agcccgccgc gaaaaactcg
     4981 ccgccgaaaa ggcgcaggcg gaacgcgaaa acggcgcggc ggatgcctta tgcgccgcgc
     5041 aggcaagcct cacgatggac gaagacgact accaccgcat caaagggctt ttgggcaaat
     5101 ggtcgcacgt tgccagcagg agcgttgaaa aacgcaccgc caaagccaaa cctgccgaca
     5161 aaacctaccg cgtcgtcctg cccgtttccg ccgatgccga aaatcaggcg gcggagctgt
     5221 ctgccaaagg tttcaacccc ataccgtttg acggcgcatt gagtttgggt gtcggcaaca
     5281 gtcgggaaaa cgcccaagcc ctgcaaaacc ggcttgccgg cgccggattc ggcggggcgc
     5341 atattgtcga acactttgcc gaagccgaca ggcaggacga ttctttgagc gtgtcgcgta
     5401 tgacggtttt gtttaccggc gtgaatgccg ccgatgcgga cgaaatccgt aaaatcacgt
     5461 ccctatacgg caaactgaac ctcaagtctt gcaaataggc gcgaaagccg gacgataaac
     5521 gccaaaccca ccgcgaaggt gggtttggcg tttcggtttt gagtatctga tatataaaaa
     5581 gggaatgtat gtctcgtata tgccgactcc aagtgtgaaa gtgatgatgg ggaaatatgg
     5641 tgtttaatgg ttcggaacgg acggcttccg agtggttttg attatacgct aataataata
     5701 attatcaata aaaatttaaa aataaatgca gattttttgt aatacgatga aaaataaaca
     5761 aattaacctg tccgccgatg tttgtgattc gggtcggcaa actttcattg tgtcggacaa
     5821 atcggggtcg gcaggcatag ctgagggctt cgtgcctgac ggaaataagc ctgccgtgtt
     5881 tgaacatccg tccgatttgg cgcggacagg gttgtcattc gggggtgttt gagcgtggat
     5941 tcggggatgc aaaagcggta aaaccgttgc ggatgaaggc ttacggacgg ctttaggtat
     6001 ttttaagtgt attcaaacaa caacaaaacg cttcttctat tgccgtattt agggcttgcg
     6061 cggcagcctg cacaccgtgg aggccgagtg tttcgttggt tgcggcatcg ggacgggcgg
     6121 caaggttttc cggccggttt ttccattttt tccgacagag ccgtgatgct tgggaatggc
     6181 ggtttgcccg tttcagaccg ccgccgtttt gggctgcgat gccgtctgaa ccgttcagac
     6241 ggcatttttg ccgccttttt caatattccg ccgaatgcca aaacggctgt ccgactgtcg
     6301 gcgtgctggc ttgcgctttg tcgcgtgctt cgaccggtcc ggcttcaaat gccagccgtc
     6361 cggattcgac ggcgagtgcg aaggcgcgtg ccatattgac cggatcgccg ctgcgggaaa
     6421 cggcagtatt caaaagcacg ccgtcaaaac cccattccat cacttgtgcc gcctgtgagg
     6481 gcaaacccaa gcccgcgtcg ataatcagcg gcgtgtcggg caggcgttcg cgcaggacgt
     6541 tcaacgcgta ggcgtgaacc gcgcccaaac ccgtgccgat cggagccgcc cacggcatta
     6601 acgcctgaca gcccacatcg agcaggcggc ggcaggcaat caggtcttcg gtgcaataag
     6661 gcagcacttt gaagccgtct ttaatcagga tttccgccgc ttcgacaagt tggaacacat
     6721 ccggctgcaa ggtgtcgtcg tcgccgatga gttcgagttt aatccaatcg gtttcaaaca
     6781 cttcgcgcgc catttgcgcc gtcgttaccg cttcctgcac gctttggcag cctgccgtgt
     6841 tcggcaggac gggaacgccg gtttcttgaa gcagcgacca aaacccctga ccgtgcgcct
     6901 cgccgccgct tcccgtgcgg cgcagcgaga cggtaatcat cgcgggttgg gcgatacgga
     6961 cggattgttt gaggatttca ggggtcgggt aggcagccgt gccgagcagc agccgtgaag
     7021 ggaaagtttc tccgtatagg gtgagcataa tgggtt
//
LOCUS       DAWKOD010000101         7032 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000101 DAWKOD010000000
VERSION     DAWKOD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.101"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(94..264)
                     /locus_tag="WJK73_001502"
                     /pseudo
     CDS             complement(94..264)
                     /locus_tag="WJK73_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(293..622)
                     /locus_tag="WJK73_001503"
     CDS             complement(293..622)
                     /locus_tag="WJK73_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7557970.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(700..918)
                     /locus_tag="WJK73_001504"
     CDS             complement(700..918)
                     /locus_tag="WJK73_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ7557971.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            1040..1993
                     /locus_tag="WJK73_001505"
     CDS             1040..1993
                     /locus_tag="WJK73_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7557972.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCYYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2068..4125)
                     /gene="metG"
                     /locus_tag="WJK73_001506"
     CDS             complement(2068..4125)
                     /gene="metG"
                     /locus_tag="WJK73_001506"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ7557973.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDIWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKADGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAKDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CGRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSDSALLAKLAAESDTIAEQYESREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(4241..6079)
                     /gene="glmS"
                     /locus_tag="WJK73_001507"
     CDS             complement(4241..6079)
                     /gene="glmS"
                     /locus_tag="WJK73_001507"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ7557974.1"
                     /translation="MCGIVGAIRAHHNVVDFLTNGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKHLNGSLGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEDLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGLIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6361..6879
                     /locus_tag="WJK73_001508"
     CDS             6361..6879
                     /locus_tag="WJK73_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557975.1"
                     /translation="MKQMLWAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIDHQKKCGQTEQAYRDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLL"
BASE COUNT         1468 a         1666 c         2118 g         1780 t
ORIGIN      
        1 tttttctgcc atttgccgtg attcgggcaa tactcggtaa cggctgattt tttgaaagtg
       61 tttgaaatcg gcgcgtggtg tttttgtgca aacggcagat gacgcatcgc ggcaggttga
      121 cggcaggtgc ttggtgttga tgttgtcggc ggcggtgcgc gtaaccggct gtccgcagaa
      181 ccattttacc gaaccgtctt gacgcttggc ccacagggag agttttttgt ctttgatttc
      241 gttgtttacg ttgcttgaag ccattatgtc agacggtatt gcccgggcag ctttattcgt
      301 acactttcag cagctcgact tcaaatatca aagtggcgtg cgggggaatc acgccgcccg
      361 cgccgtgtgc gccgtagccc atttccgaag ggatggtcag cttgcgtttg ccgccttcct
      421 tcattccgcc gaagccttcg tcccagcctt tgatgacttg tccgacaccg agcgtgatgg
      481 tcagcggctg gcggcggtcg aggctggagt cgaatttggt tccgttttcc agccaacctg
      541 tgtaatgcac ggtaatctct ttgcctttaa ctgcttcttt tccgaagcct tcttgcaagt
      601 cttcaataat caggccgccc atatttgtcc tttcattgct tgttggtcaa aacggcaagg
      661 gtaacatacc gtccgtcgaa gtcaaatgcc gctcaaacgt cagctgcatc ggtgcagctg
      721 aaacggctgt gtttgttgga ctgttttatt tttttcgtaa aggttccatg ctttttcatg
      781 gaaatagaaa acgacggtgt tgattagggg ctcgaccagc gcgaccgccc ccgatacgcc
      841 tatactgccc gtcagtacat aggttacact gaaggcggtg ctgaaatgca gtgcggcaaa
      901 agtcagggtt ttaagcatca tcctctcccg aattggacat tgacggagga atgataaagg
      961 ttatcataag gttgcgcggt ttaaatttgc tatttgttgt tagtgtaaat aaatcatttt
     1021 tcaaatgggg atagaaatta tgaatcataa aaagatcgtt gttttggatg cggatacttt
     1081 gcccggccgg gtttttcatt ttgattttcc gcacgagctt gcggtttacg gtacgacagg
     1141 tgcggatgaa acggcagaac gggtgcgcga tgcacatatt gtcattacta acaaagtgat
     1201 gatttctgcc gatattattg cggctaatcc gcagttggag ctgattgccg tcagtgcgac
     1261 cggtgtgaac aatgtcgata ttggggcggc gaaggcggcg ggcgttgcgg tatgcaatgt
     1321 ccgcgcctac ggaaacgaat cggttgcgga acacgccttt atgctgatga ttgcgttgat
     1381 gcggaatttg cccgcctatc agcgtgatgt tgcggcagga ttatgggaga agtcgccgtt
     1441 tttctgctat tacggtgcgc cgattcggga tttgaacggt aaaacgctgg cggttttcgg
     1501 acgcggcaat atcggacgga cgcttgccgg atacgcgcag gcgttcggta tgggggtggt
     1561 gtttgccgaa cacaaacacg cgcccgacgt gcgcgaaggc tatgtttcct ttgaagatgc
     1621 ggtacagact gccgacgtgt tgtcgctgca ctgtccgctg aacgcccaaa ctgaaaatat
     1681 gataggcgaa aacgaattac agcagatgaa gcccggtgcg gttttgatta attgcgggcg
     1741 cggcgggctg gtggatgaaa acgcgctgct tgccgcactc aaatacgggc agatcggcgg
     1801 ggcgggtgtc gatgttttga cgaatgagcc gcccaaaaac ggcaatccct tgctgaatgc
     1861 acgattaccc aatctgattg ttacgccgca taccgcgtgg gcaagtcgtg aggctttgga
     1921 caggctgttt gatatattgt tggcgaacat tcacgccttt gtgaaaggag aggcgcaaaa
     1981 ccgcgtggtt tgagcttgtc gggatgacgg aaaacaatgc cgtctgaacg cccaaagggt
     2041 tcagacggca ttttttgaga ttcctgttta accgactttg tcgcccggct gcgcgcctgt
     2101 atccacatct aagagcttca gtttcccgtc tgccgtggcg gcactcaaaa tcatgccttc
     2161 ggatacgccg aattttgcca ttttgcgcgg ggcgaagttg gcgacggcga tgaccattct
     2221 tccgtttaac tcggcagggt tggggtaaaa cgcggcgatg ccggagaaga tgatacgttt
     2281 ttcaaaaccg aaatcgaggt cgaatttcaa aagtttggtg ctgccttcga cggcttcgca
     2341 gttcaatact ttggcgacgc gcatatcgat tttcatgaag tcgtcgaaac ttgcctgttc
     2401 ggcgactttt tcgtatttgc cctcttcggc ggcaggtgcg gctgcggcgg cgatgctttg
     2461 tttgttggct tcgattaaat cgtccacttg tttttgctcc actcgttgca ttaaatgttt
     2521 gtatttgttg atggcgtgtt tgcccaaggt ttcgcgtgta tttgcccaag tgatggcttc
     2581 caaattcagg aatttggcgg cgtttgcggc ggtttgcggc aagacggggg cgaggtaggc
     2641 ggtcaacatg gtgaaggcgt tgatgagctc gctgcacact tcgtgcaggc gttcgtcttg
     2701 accttcctgt ttcgccagtt cccaaggttt gttggcatcg acgtattcgt tcacggcgtc
     2761 tgccaatgcc atgatgtcgc gcagggcgcg ggcgtattcg cggctttcgt attgctcggc
     2821 gatggtgtcg ctttcggcgg cgagttttgc cagcaatgcg ctgtctgaaa cgtctttcag
     2881 acggccttca aagcgtttgg cgatgaagcc tgacgcgcgg gcggcgatgt tgacgtattt
     2941 gccgacgagg tcactgttga cgcgcgaaat aaagtcttgc aggttcaaat cgatgtcttc
     3001 gattttgctg ttgagtttgg cggcgatgta gtagcgcatc cactcggggt tcaggccttg
     3061 ttccagatag gatttggcgg taataaacgt gccgcgcgat ttggacattt tttgtccgtc
     3121 gacggttagg aagccgtgtg cgtacacgcc ggtcggggcg cggtggccgg agaaatgcag
     3181 catagcgggc cagaacaggg cgtggaaata gaggatgtct ttgccgatga agtggtacat
     3241 ctcggtttgg ctgccggctt tgaagtattc gtcgaaatcg atgccgatgc ggccgcacag
     3301 gtttttaaac gatgccatgt agccgacggg cgcgtccagc cagacgtaga agtatttgcc
     3361 cggcgcgtcg gggatttcaa aaccgaaata tggcgcgtcg cgggaaatgt cccagtcgga
     3421 cagggtggtt tcttcacctt cacccagcca ttctttcatt ttgttgaggg cttcggcttg
     3481 cagatggggc ttgccgtcgt gcgggttgtt gccggaagtc catgctttga ggaagtcggc
     3541 gcattcgccc agtttgaaga agaagtgttc ggattcgcgc aattcgggtt tggcaccgga
     3601 aacggcagaa tacgggttga tcagttcggt cggggaatag gtcgtgccgc agacttcgca
     3661 gttgtcgccg tattggtctt tggcgtggca tttcgggcat tcgcctttga cgaagcggtc
     3721 gggcagaaac atttgttttt cagggtcgaa aagctgctcg atgacgcggc tctcgatttt
     3781 gccgtcggct ttcagtgcac ggtaaatgtc ttgggaaaac tgtttgtttt caggagaatg
     3841 ggtgctgtaa taattgtcgt agccgatgaa aaagccggta aagtcggcga ggtgttcttc
     3901 gcgcactttg gcaatcatgt cttcgggcgc gatgccttgt ttttgcgcgg caagcatcac
     3961 gggtgtgccg tgggtgtcgt cggcgcagca gtagtggcat tcgtggccgc gcagtttttg
     4021 gaagcgtacc caaatgtcgg tttggatgtg ttcgaccatg tggccgaggt ggatgctgcc
     4081 gttggcatac ggcagggcgg aggtaacgag gattttgcgt gtcatattgt gctttgcaaa
     4141 caatgggtaa aggcggatta taccgcaaat caaacgggga aatgccgtct gaagcctgaa
     4201 aaatcgggct tcagacggca tttttgccaa ccggcgggat ttattcgacg gttacggatt
     4261 tcgccagatt gcgcggcttg tccacatccg tgccgcgtgc gagggcggtg tggtaggcga
     4321 ggagctgcac ggggatggta tgcacgacgg gggacagttc gccgacgtgg cgcggtgcgc
     4381 ggataacgtg cacaccttcg gtggcattaa aattgctgtc gaggtcggca aagacgaaaa
     4441 gttcgccgcc gcgcgcgccg acttcctgca tattggcttt gactttgtcc aacaggctgt
     4501 cgttgggtgc gatgacgacg acgggcatgt tttcgtccac caaggcaagc ggcccgtgct
     4561 tcagttcgcc ggcaggatag gcttcagcgt ggatgtaggt gatttccttc agttttaacg
     4621 cgccttcgag ggcaatcggg taatggatgc cgcgccccaa aaacagcgcg ctggttttct
     4681 tggcaaactg ttgcgcccag gcggcaattt gcggttcgag gtttaaagcg tgctgcacgc
     4741 tgccgggaag ctggcggagt tcttcggtgt aacgtgcttc gtcttcttcg gaaaccaaac
     4801 cgcgcacttt cgccagcgtt accgccaaac cgaacagcgc aaccagctgc gtggtaaatg
     4861 ctttggttga ggcgacgccg atttccgcac cggcacgggt ataaagcacg aggctgcttt
     4921 cgcgcggcag ggcggattcc atcacgttgc aaatggagag gctgtggcgg tgtcccaacg
     4981 atttggcgta tttcaaagcc tccatcgtgt ccagcgtttc gccggattgg gaaatggtaa
     5041 tgaccagttg gtcggggtcg gcaatcacgc tgcggtatcg gtattcgctg gcgatttcga
     5101 catcggccgg gattttggcg atggattcca accaatattt ggcggtcagc gcggcgtaat
     5161 aggacgtgcc gcaggcaagg attttgacgc tgcggatgct ttcaaacacg cttttggcgt
     5221 ctttgccgaa gttttcgggg atgaggccgc cgtcgaggaa aacttccgcc gtatccgcaa
     5281 tcgcgcgcgg ctgctcgtgg atttcctttt gcataaagtg gctgtacggt cccagttcca
     5341 aagaggcgag cgagagttcg gataccttga ctttgcgttc ggcaggcagg ccgtttttat
     5401 cggtcagcct tttaatgccg tctgaagcca gcagtgcgat gtcgccgtct tcgaggtacg
     5461 ccacgcggcg cgtaaaggcg atgacggcgg acacgtccga agcgataaag gtttcatcgt
     5521 cgcccaaagc gaccaaaagc gggcagccca tacgcgccac aactaattca tcaggcttgt
     5581 cttgggcaat aaccgcgatg gcgtatgcgc cgtggaaacg tttgaccgct tcctgtaccg
     5641 cttcaaacag cctgccgccg ttttgcgcgt attcgtgatt gatgctgtgg gcgatgactt
     5701 cggtatccgt ttgcgattca aaacggtatc ccaaatcttc caagcgttta cgttcgcttt
     5761 caaagttttc gatgatgccg ttgtggacga cggcaatcat accgccgctg atgtgcgggt
     5821 gggcgttcgg ctcggtaacg ccgccgtgtg tcgcccagcg cgtatgcccg atgcccaaac
     5881 tgccgttcaa atgcttttca cgcgccgcat cttccattaa ctgcacgcgc ccgacacggc
     5941 gcacgcgctt gattttgccg tctgaataca cggcgatgcc cgatgaatcg taaccccggt
     6001 attcgaggcg tttgaggccg ttggtcagaa aatcgacgac gttgtgatgg gcgcggatgg
     6061 cgccgacgat accgcacatg actgttcctt agtatccggt tgaaaaaaaa caggcgcgga
     6121 cggctcccgt gccgcacctt ccgcttcgga ttataaaccg ccttccgcgc cgaaaaacag
     6181 caaaatgccg tctgaaggct tgggcttgct caaaaaaggg agggatttcc ccgcctgacc
     6241 gggatgggcg ttcagacggc attgcctgct gccggtttat agtttttgca aatcaatatt
     6301 gacaagctga aaaaaaaaaa caatatactc gcccagtctt aatgttaacg gagtatggaa
     6361 atgaaacaaa tgctttgggc cgtcggcgtg gcggcggtgt tggcgggctg cggcaaggat
     6421 gccggcggtt acgagggtta ttggcgtgaa aagtcggaca aaaaagaggg tatgattgcc
     6481 gtcaaaaaag aaaaaggcaa ttacttcctt aataaaatca acgtgtttac aggtaaggaa
     6541 gaatctatgc ttttgtctga aaaagacggc gcgctttcga taaacacggg gataggggaa
     6601 atcccgatca aactttccga cgacgggaaa gagctgtatg tcgaacgcag gcagtatgtg
     6661 aaaaccgatg cggcgatgaa ggacaaaatc atcgaccatc agaaaaagtg cggacaaacg
     6721 gaacaggcat accgcgacgc gcgaaatgcg ttgccgtcaa accaaacgta tcagcagcgt
     6781 caggcggcga tcgagcagtt gaaacggcgg tttgaagccg agtttgacga attggaaaaa
     6841 gaaatcaaat gcaacggcaa accgacattg ttgctttagc gtaggacaac cgggaggatg
     6901 ccgccgtccg aatcggacgt gcggtttccg taccggtacg ggcgggcagg aatgtccgct
     6961 ttttttgttc ggacgcgttt gaatatccgt ccgattccgt ccgtttgcaa agggtatttt
     7021 tgttcgggcg ga
//
LOCUS       DAWKOD010000102         6997 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000102 DAWKOD010000000
VERSION     DAWKOD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.102"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(74..1495)
                     /locus_tag="WJK73_001509"
     CDS             complement(74..1495)
                     /locus_tag="WJK73_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ7557976.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1853..2212)
                     /locus_tag="WJK73_001510"
     CDS             complement(1853..2212)
                     /locus_tag="WJK73_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ7557977.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2209..2814)
                     /locus_tag="WJK73_001511"
     CDS             complement(2209..2814)
                     /locus_tag="WJK73_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ7557978.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2861..3343)
                     /locus_tag="WJK73_001512"
     CDS             complement(2861..3343)
                     /locus_tag="WJK73_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ7557979.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3547..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="WJK73_001513"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="WJK73_001513"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ7557980.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGIEAVVFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEADVRVLKLPFVRNGQKQFD"
     gene            5117..5503
                     /gene="gcvH"
                     /locus_tag="WJK73_001514"
     CDS             5117..5503
                     /gene="gcvH"
                     /locus_tag="WJK73_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ7557981.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPSAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            5649..6896
                     /locus_tag="WJK73_001515"
     CDS             5649..6896
                     /locus_tag="WJK73_001515"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ7557982.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRKSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1785 a         1681 c         1953 g         1578 t
ORIGIN      
        1 gtcggaaggg gggatattga tttgattatg ccggaattta aaatgccgtc tgaatgttca
       61 gacggcatag cgtttacagc agtttgaaaa cgaaaaagat aagggtatgt acgatgaaga
      121 cgggtgtcag gaaggcgacc gaccacatca tatagccgaa gaaagtcggc atcggtacgc
      181 cgcgctgttc ggcaatggcc ttgaccatga agttcggtgc gttgccgatg taggtcagtg
      241 cgcccatgaa taccgaaccc atagaaaccg ccagcagcga atgaaacagg gaacccgtca
      301 tcaaggcttg ggcatcgccg cccgccatat tgaaaaaaac gagataagtg ggcgcgttat
      361 ccaagaatgc cgacaatatg ccgctcatcc aaaaatacat cacattaatc ggatgacctg
      421 ccgtatcgtg aaccagcgat accaccccgc ccagcgcgcc tgcctcgcct gctttcagaa
      481 tgctcaggac gggaaagatg gtgatgaaga tgccgaggaa gagtttgccc acttcggcga
      541 tgggttcaaa gttgaattcg ttgcctgcgc ggacttgttt gggcgtgatt gccatagata
      601 cggcggtcaa tgcaatcagg atgacatcgc ggacgaggtt ttgcagggcg taacggctgc
      661 cgaggatttc aaatcccggg tgttcgggtt tccaaaggcc ggacattaga accgcgccga
      721 ccacgcccga aagcaggagg aagttccatt tgccgaagat ggcgattttt tcgggttttt
      781 cctgttgtgc cggcgtatct tgtgcaatgc tttcctgttt gaagaaacgg ttgtcgatga
      841 aatagaaggc ggtcaacagg acagcggtgc tgatcaggac gggggcgaac atatgtttga
      901 ccgtccacat gaaatctacg cctttgagga agccgaggaa gagtgggggg tcgcccaaag
      961 gggtcagacc gccgccgatg tttgcaacca ggaaaatgaa gaagatgacg atgtgcacgc
     1021 ggcgggtacg gttttggttg gctttcagca gcggacgaat catcagcatt gctgcgccgg
     1081 tcgttcccat gatagaggca agtgccgtac cgacggcaag cagggcggtg ttgagcttgg
     1141 gtgtgccgtt caagtcgccc caaaccaaaa tgccgcctga aatggtgtac agggcaagca
     1201 gcagcaggat gaaagggatg tattcttcaa cgagtgcgtg tgcgacggta tggataccgg
     1261 cggacgcgcc aaaaaccaaa ctgaacggga tgaggaagag caatgtccaa aaggcggtaa
     1321 ttttgccgta atggtgatgc caggtatgcg aaaaaaacaa gggacccaat gcgatagaca
     1381 gcaaaatcag ggcaaagggc aggctccaca gcaggtttag gtttgcgccg tccaaatctg
     1441 cggcgtaaac cgatgcgggg aaaagcatta gtgaaaacag gggtaggtgg cgcatcgtgt
     1501 ttcctcgatt caagcactgc cttgcgcggc gcgtgggagt gatacaggca tcgtgccgcc
     1561 cggacatagg cggactgtgc tgcctgtttg tttttgaaaa aagttttccg attgattgta
     1621 aagtaaataa tcaggataaa ccagtttccc aaaccggaag gcggcgggaa ggcggattgc
     1681 tgtgcttggg aatatatgtt ctttttgata aataatttta tttaaaacaa ataaatatat
     1741 gtctttaata aataatctat cgaaaacgaa aaatgaattt attttaacat atatttgcaa
     1801 tgaaacaggt ttgccccccg tttgtttgcc cttatccctt tcagtacggc attcaagatt
     1861 cgggcctgcg ccacatccat atggcgacaa gggaacaaaa aaccgatgaa accgccccga
     1921 cccaccagcg ttggggaaac tgccaaaaca ttatcaggca ggatgcggtc atcatactga
     1981 tggcgaatat tttggctttg cgcggcactg cgccgttttg ttcccagtta tgaaccatcg
     2041 gaccgaaata gcggtgccgg tgcagccagc ggtgaaagcg cggggatgcc tttgcccagc
     2101 aggcggcgga gagcagtacg aacggcgtgg tcggcaacag cggcaaaaaa atgccgatga
     2161 tacccaacag cagggaaatg cagccgcagg caattaaaag ataacgtatc attttgaaat
     2221 atttttctta ttgtgcggat aagggcagga tgtgataccg agttttgccc agccttcatg
     2281 tcccattttt tccagcaggg cgatattgcg ttcgaatatt gccgaagtgt cgggaaaggc
     2341 ttgtgcggct ttggcaatgc tgtcttcgcg gatgaggtgc agcgtcggat agggagaacg
     2401 gttggtgtag ttgccgatgt cgtcgggttc ggtattctga aagcggaatt ggggatggaa
     2461 cggtgcgatt tggacgatgc cttccaagcc gttttcgata acggcggcat cggcaatatc
     2521 gagcatatcg ttgaatacgt cgaaatcggg gaatagggtc gggtgaacca gcagggtggt
     2581 ttccagttcg gtggcgggtg tattgcccag tcgctgcagt tcttcgtcca agtcttccaa
     2641 aaaactgtca aggtgtttgg cttcgctgat tgcgatgcgg acaaggtttt taacgtgggg
     2701 ggctttggca aagggacaca ggttcagacc gatgacggct ttttccaacc attgtccggt
     2761 gtgttcggca acagcatctt tattttcgga agtattgata ttcattattg tcatgtaaat
     2821 gtgtttgcag attgcacgtg cgggaaaatc gggaagggca ctattccttc agcaggtggt
     2881 tgagcggcag ggaggtggtg tgtttgattt cttttaaaac aaagctcgat tgcgcatctt
     2941 gtacgccgtg gtgggacagg agcgtatcca aaacaaaatg ggaaaacgcg ttcatatcgg
     3001 taaaaaacgc ctgaagcagg tagtcggttt cccctgtcag ggcgaagcag ctcaagactt
     3061 caggccattt tcggaccgat gcggcaaagt cttcccgcgc gtcttttgct ttgcggatgg
     3121 aaacgcggat aaatgcctga agccccaggt tgacggattc cggagacagc agcgcggcat
     3181 attggcggac gatgccggca tcttccaact gctttagacg gcgcaggcac ggagaaggcg
     3241 aaagtgcgac acgttcggac agttcgacat tggtcagcct gccgttttcc tggagaacct
     3301 gtaagatttt aatatcggtt ttgtctaaag tgagttgggg catatttgcg ttccgtttta
     3361 aggaattcgg attgtctgtc cgtatgtttg cggcaatccg cacagatgga gaccatatta
     3421 acatataaaa agttataccg tcatccggga caaattttgt tttcggaaaa tcatgtgaaa
     3481 acagaggcgg tcggtttgca tctcttttaa gacggcttgc ccaaaccgcc gattcaagac
     3541 ataatcggga aatgtgcagg agagtgttac acccaactac aatgtaacca ccgaaggcgc
     3601 agacaccctt aaatcgctca ggtatcaggg actgcacatt gaaacaaaca atctggagag
     3661 cggcgttgga ataacgtcca ccgaagggga gaaggccgtc tgaaccacca ttcagacaac
     3721 cgcgcaaagc agtgagcaga ctggtttgcc atcatgcgga tacggccgaa aatctcaggt
     3781 tcaaggacag atagggtcat ccgcgcacag gtgcgcgggt ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gccggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttaatc gtttaccgca ccaacgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg cgcgactcgc gaaaaagata cggcacaatt ccacaaagtc ggacaagagt
     4261 tcggtgtttc cttcaatccg cgctacgacc tcggcatgct cgccgtacaa ggtcctaaag
     4321 ccattgaaaa actcctgacc gtcaaacccg aatgggcaga tgtcgtccat aacctcaaac
     4381 cgttccaagg cgcggatttg ggcaacgact ggtttgtcgc ccgcaccggc tacaccggcg
     4441 aagacggcgt cgaagtcatc ctgcccggca tcgaagccgt cgtattcttc aaagccctgc
     4501 aacaagccgg cgtacagccc tgcggcctcg gcgcgcgcga taccctgcgc atggaagccg
     4561 gtatgaacct ctacggcaac gatatggacg acgacaccag cccgcttgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgatttcgt cggtaaagcc gccttgctgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggtct gttgctcgac aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tcttctctcc aagcctgaaa caatccatcg ccatcgcccg cgtgccgaaa gactttgacg
     4861 gcgataccgc caaagtgctg atgcgcggca aagaagcgga cgtgcgcgta ctgaagctgc
     4921 cgttcgtgcg caacggacag aaacagtttg attgatgcgg tttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gttgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aacccctatc
     5101 aatggagtat caaaccatga gcaacaacat cccggccgaa ctgaaatacg ttgccagcca
     5161 cgaatggctg cgccttgaag aagacggtac catcaccgtc ggtattaccc accacgcgca
     5221 agagctgttg ggcgacatcg tgttcgtcga gctgcccgaa gtcggcgcaa accttgccgc
     5281 tgaagagcaa gccggtgtgg ttgagtctgt aaaagccgcg tccgacgtgt acgcaccgat
     5341 tgcaggtgaa gtcgttgccg tcaacgatga cttacccagt gcacctgaaa ctgccaacag
     5401 cgacccttat ggcgcaggct ggttcttcaa aatcaaacct gccaatcctg ccgattacga
     5461 cggtctgctg actgccgaac aatacgcggg cgaagtagat taaattccta tcctgataat
     5521 aggatgcctt atttaaggaa ctgcactttc agacggcaag gatgcgtttg gaagtgcagt
     5581 ttttgtctgt gtcttcaagg tcagttaggc tataataacg gtctatattc atctttaccg
     5641 attttttcat gcaacttacc gctgtcggac tcaatcatca aaccgcacct ttaagcatac
     5701 gggaaaagct ggcgtttgcc gcggcctgcc tgcccgaagc cgtccgcaat cttgcccgaa
     5761 gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa ccgcaccgag ctttactgtg
     5821 taggcgattc ggaagaaatc atccgttggc tcgcagacta tcacagcctt cccatagaag
     5881 aaatcagccc ctacctttat actttgggga tgcaggagac tgtgcgccat gctttccgcg
     5941 tcgcctgcgg cttggattcg atggtgttgg gcgagccgca gattttagga cagattaagg
     6001 atgcggtcag ggttgctcaa gagcaggaaa gtatgggtaa gaaactcaat gccctgttcc
     6061 aaaaaacctt ttctgttgct aaagaggtcc gtaccgatac tgccgtcggc gaaaactcgg
     6121 tttccatggc ttccgcttcc gtcaaattgg cggaacagat ttttcccgac atcggcgatt
     6181 tgaatgtctt gtttatcggc gcaggcgaaa tgattgagct ggttgccact tattttgccg
     6241 ccaaaagtcc ccggctgatg acggttgcca accggacgct ggcgcgtgca caggagttgt
     6301 gcgacaagct cggtgtcaac gccgaaccgt gcctgctgtc cgatctgcct gccattctgc
     6361 acgattacga cgtagtggtt tcttcaacgg caagccagtt gcccattgtc ggcaaaggca
     6421 tggtggagcg tgcattgaaa caaaggcaga gtatgccgtt gttcatgctt gatttggcag
     6481 tgccgcgcga tattgaagcg gaagtcggcg atttgaacga tgcctatctt tatacggtgg
     6541 acgatatggt caacatcgtc caaagcggca aggaggcaag gcagaaggcc gccgccgccg
     6601 ccgaaacgct ggtgtccgag aaggttgccg aatttgtccg gcagcagcag ggcaggaaga
     6661 gcgtcccctt gattaaagcc ttgcgggacg agggcgagaa agcgcgcaag caggtgttgg
     6721 aaaatgcgat gaaacagctt gctaaaggcg caacggcaga agaggttttg gaacggctgt
     6781 ccgtccaact gaccaacaag ctgctgcatt cgccgaccca aactttgaat aaggcggggg
     6841 aagaagataa ggatttggtt catgccgtcg cgcagattta tcatttggac aaataacggt
     6901 gcgccgggaa aaatgccgtc tgaagaggtt tcagacggca tttttttgtg ccgcctgaca
     6961 acatcgtaaa atcccacatt atatcgatgt aatcaca
//
LOCUS       DAWKOD010000103         6782 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000103 DAWKOD010000000
VERSION     DAWKOD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.103"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJK73_001516"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJK73_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ7557983.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1552..1707
                     /locus_tag="WJK73_001517"
     CDS             1552..1707
                     /locus_tag="WJK73_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557984.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1704..2135
                     /locus_tag="WJK73_001518"
     CDS             1704..2135
                     /locus_tag="WJK73_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ7557985.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2269..6618
                     /gene="app"
                     /locus_tag="WJK73_001519"
     CDS             2269..6618
                     /gene="app"
                     /locus_tag="WJK73_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ7557986.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
BASE COUNT         1792 a         1973 c         1678 g         1339 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accgccacga
       61 tgctttcgat ccgtttattt tccgatgcag aatctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg catgcgacct gagccaagcg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca gcccttcgct ttcgccctgc
      301 caaccggcct cacgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc agttttcgcc
      361 gacagcgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccggcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atgcgttcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccagcgcg
      721 ttcaacaggc tggatttgcc gacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatcc
      901 agcgtcgctt cgaccagcat ccgcaaggta atcaaatctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaac gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgtttggtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgataacatc ttcgcccgta
     1201 aaacttgccg gtgcggcaaa aaacagcaaa agcccgctgt cgattgcctg accgtccgcg
     1261 tccgtaaaat cagcataggt tgcggtacgc ggtttgggcg ttttcccaca caaagcctct
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgcccgggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatataacca tatggaaaaa
     1561 cgaaacacat acgccctcct gctcggtata ggctcgctgt tgggcctgtt ccatcccgca
     1621 aaaaccgcca tccgccccaa tcccgccgac gatctcaaaa acatcggcgg cgattttcaa
     1681 cgcgccatag agaaagcgcg caaatgaccg aaaacgcaca ggacaaggcg cggcaggctg
     1741 tcgaaaccgt cgtcaaatcc ccggagcttg tcgagcaaat cctgtccgac gagtacgtgc
     1801 aaataatgat agcccggcgt ttccattcgg gaccgttgcc gccgccgtcc gacttggcgc
     1861 aatacaacga cattatcagc aacggggcag accgcattat ggcaatggcg gaaaaagaac
     1921 aagccgtccg gcacgaaacc atacggcaag accaaacctt caacaggcgc gggcaactgt
     1981 acggcttcat cagcgtcatc ctgatactgc tttttgccgt cttcctcgta tggagcggct
     2041 accccgcaac cgccgcctcc cttgccggcg gcacagtggt tgccttggcg ggtgctttcg
     2101 tgattggaag aagccgagac caaggcaaaa attaatgaca aatcctaggg cgtgcttcat
     2161 atccgcccga acgccgaacc gcacatatag gcacatcccg cgcgccgccc ggaagcggaa
     2221 gccgcgccct cccaaacaaa cccgaatccc gtcagataag gaaaaataat gaaaacaacc
     2281 gacaaacgga caaccgaaac acaccgcaaa gccccgaaaa ccggccgcat ccgcttctcg
     2341 cctgcttact tagccatatg cctgtcgttc ggcattcttc cccaagcctg ggcgggacac
     2401 acttatttcg gcatcaacta ccaatactat cgcgactttg ccgaaaataa aggcaagttt
     2461 gcagtcgggg cgaaagatat tgaggtttac aacaaaaaag gggagttggt cggcaaatca
     2521 atgacaaaag ccccgatgat tgatttttct gtggtgtcgc gtaacggcgt ggcggcattg
     2581 gtgggcgatc aatatattgt gagcgtggca cataacggcg gctataacaa cgttgatttt
     2641 ggtgcggaag gaagaaatcc cgatcagcac cgtttttctt accaaattgt gaaaagaaat
     2701 aattataagc ctgacaattc acacccttac aacggcgatt accatatgcc gcgtttgcat
     2761 aaatttgtca cagatgcaga acctgtcgaa atgacgagtg acatgagggg gaatacctat
     2821 tccgataaag aaaaatatcc cgagcgtgtc cgcatcggct caggacacca ctattggcgt
     2881 tatgatgatg acaaacacgg cgatttatcc tactccggcg catggttaat tggcggcaat
     2941 acacatatgc agggttgggg aaataatggc gtagttagtt tgagcggcga tgtgcgccat
     3001 gccaacgact atggccctat gccgattgca ggtgcggcag gcgacagcgg ttcgccaatg
     3061 tttatttatg acaaaacaaa caataaatgg ctgctcaacg gagttttaca aaccggctac
     3121 ccttattccg gcagggaaaa cggtttccag ctgatacgca aagattggtt ctacgatgac
     3181 atttacagag gcgatacaca taccgtcttt tttgaaccgc gcagtaacgg acatttttcc
     3241 tttacatcca acaacaacgg tacgggtacg gtaacagaaa ccaacgaaaa ggtttccaat
     3301 ccaaagctta aagtacagac agtccgactg tttgacgaat ctttgaatga aactgataaa
     3361 gaaccagttt acgcggcagg gggtgttaat cagtaccgtc caaggttaaa caacggtgaa
     3421 aacctttctt ttatcgatta cggcaacggc aaactcatct tatcaaacaa catcaaccaa
     3481 ggcgcgggcg gtttgtattt tgaaggtgat tttacggtct cgcctgaaaa caacgaaacg
     3541 tggcaaggcg cgggcgttca tatcagtgaa gacagtaccg ttacttggaa agtaaacggc
     3601 gtggcaaacg accgcctgtc caaaatcggc aaaggcacgc tgcacgttca agccaaaggg
     3661 gaaaaccaag gctcgatcag cgtgggcgac ggtacagtca ttttggatca gcaggcagac
     3721 gataaaggca aaaaacaagc ctttagtgaa atcggcttgg tcagcggcag gggtacggtg
     3781 caactgaatg ccgataatca gttcaacccc gacaaactct atttcggctt tcgcggcgga
     3841 cgtttggatt taaacgggca ttcgctttcg ttccaccgta ttcaaaatac cgatgaaggg
     3901 gcgatgattg tcaatcataa tgccacaaca acatccaccg ttaccattac agggaatgaa
     3961 agtattacac aaccgagtgg taagaatatc aatagactta attacagcaa agaaattgcc
     4021 tacaacggtt ggtttggcga gaaagatacg accaaaacga acgggcggct caaccttgtt
     4081 taccagcccg ccgcagaaga ccgcaccctg ctgctttccg gcggaacaaa tttaaacggc
     4141 aacatcacgc aaacaaacgg caaactgttt ttcagcggca gaccgacacc gcacgcctac
     4201 aatcatttag gaagcgggtg gtcaaaaatg gaaggtatcc cacaaggaga aatcgtgtgg
     4261 gacaacgact ggatcaaccg cacgtttaaa gcggaaaatt tccatattca gggcgggcag
     4321 gcggtgattt cccgcaatgt tgccaaagtg gaaggcgatt ggcatttgag caatcacgcc
     4381 caagcagttt ttggtgtcgc accgcatcaa agccatacaa tctgtacacg ttcggactgg
     4441 acgggtctga caaattgtgt cgaaaaaacc attaccgacg ataaagtgat tgcttcattg
     4501 actaagaccg acatcagcgg caatgtcagc cttgccgatc acgctcattt aaatctcaca
     4561 gggcttgcca cactcaacgg caatcttagt gcaaatggcg atacacgtta tacagtcagc
     4621 cacaacgcca cccaaaacgg caaccttagc ctcgtgggca atgcccaagc aacatttaat
     4681 caagccacat taaacggcaa cacatcggct tcgggcaatg cttcatttaa tctaagcaac
     4741 aacgccgcac aaaacggcag tctgacgctt tccgacaacg ctaaggcaaa cgtaagccat
     4801 tccgcactca acggcaatgt ctccctagcc gataaggcag tattccattt tgaaaacagc
     4861 cgctttaccg gacaactcag cggcagcaag gatacagcat tacacttaaa agacagcgaa
     4921 tggacgctgc cgtcaggcac ggaattaggc aatttaaacc ttgacaacgc caccattaca
     4981 ctcaattccg cctatcgcca cgatgctgca ggcgcgcaaa ccggcagtgt gtcagacacg
     5041 ccgcgccgcc gttcgcgccg ttccctatta tccgttacac cgccaacttc ggtagaatcc
     5101 cgtttcaaca cgctgacggt aaacggcaaa ttgaacggtc aaggaacatt ccgctttatg
     5161 tcggaactct tcggctaccg aagcgacaaa ttgaagctgg cggaaagttc cgaaggcact
     5221 tacaccttgg cggtcaacaa taccggcaac gaacccgtaa gcctcgatca attgacggta
     5281 gtggaaggga aagacaacaa accgctgtcc gaaaacctta atttcaccct gcaaaacgaa
     5341 cacgtcgatg ccggcgcgtg gcgttaccaa ctcatccgca aagacggcga gttccgcctg
     5401 cataatccgg tcaaagaaca agagctttcc gacaaactcg gcaaggcaga agccaaaaaa
     5461 caggcggaaa aagacaacgc gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc
     5521 gccgaaaaga cagaaagcgt tgccgaaccg gcccggcagg caggcgggga aaatgtcggc
     5581 attatgcagg cggaggaaga gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg
     5641 aaacagcgcg aagcggaaac ccggccggct accaccgcct tcccccgcgc ccgccgcgcc
     5701 cgccgggatt tgccgcaacc gcagccccaa ccgcaacctc aaccccaacc gcagcgcgac
     5761 ctgatcagcc gttatgccaa tagcggtttg agtgaatttt ccgccacgct caacagcgtt
     5821 ttcgccgtac aggacgaatt ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg
     5881 acaagcggca tccgggacac caaacactac cgttcgcaag atttccgcgc ctaccgccaa
     5941 caaaccgacc tgcgccaaat cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc
     6001 ctgttttcgc acaaccggac cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg
     6061 cttgcccacg gcgccgtttt cgggcaatac ggcatcggca ggttcgacat cggcatcagc
     6121 acgggcgcgg gttttagcag cggcagtctt tcagacggca tcggaggcaa aatccgccgc
     6181 cgcgtgctgc attacggcat tcaggcacga taccgcgccg gtttcggcgg attcggcatc
     6241 gaaccgtaca tcggcgcaac gcgctatttc gtccaaaaag cggattaccg ctacgaaaac
     6301 gtcaatatcg ccacccccgg tcttgcgttc aaccgctacc gcgcgggcat taaggcagat
     6361 tattcattca aaccggcgca acacatttcc atcacgcctt atttgagcct gtcctatacc
     6421 gatgccgctt cgggcaaagt ccgaacacgc gtcaataccg ccgtattggc tcaggatttc
     6481 ggcaaaaccc gcagtgcgga atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc
     6541 ctccacgctg ccgccgccaa aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa
     6601 ttaggctacc gctggtaacc gccggatatg ccgaaagggg tctgacgatg ccaccgtgcg
     6661 ctgtcaaacc ctttttctgc cgccgccttg tgcctgctcc attgtctgat aaccgtcaaa
     6721 ttgattttta gcccatgttt ggtgggtcgg ggaaatctat atcttcgtcc gtgccgaaat
     6781 ag
//
LOCUS       DAWKOD010000104         6758 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000104 DAWKOD010000000
VERSION     DAWKOD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.104"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            245..2383
                     /locus_tag="WJK73_001520"
                     /pseudo
     CDS             245..2383
                     /locus_tag="WJK73_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2625..4088
                     /locus_tag="WJK73_001521"
     CDS             2625..4088
                     /locus_tag="WJK73_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7557987.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSRPE
                     FRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGIR
                     VILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAAA
                     LFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNIN
                     QAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFN
                     DMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLSS
                     AEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRYG
                     LRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRALH
                     FKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4289..5257)
                     /locus_tag="WJK73_001522"
     CDS             complement(4289..5257)
                     /locus_tag="WJK73_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557988.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5335..6627
                     /locus_tag="WJK73_001523"
     CDS             5335..6627
                     /locus_tag="WJK73_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7557989.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
BASE COUNT         1613 a         1717 c         1996 g         1432 t
ORIGIN      
        1 tcccgttatt tccacaaaac agaaaatcaa aaacagtaac ctgaaatccc gtcattcccg
       61 cgcaggcggg aatccagacc tttaaacccc gaccatcctt gataaattct tgcggcatta
      121 aaattctaga ttcccgcttt cgcgggaatg acggcggagg gttttttgct tttcctgatt
      181 tttcattgcg atgtagtata atgtagtata taatcattat aattttaaca cttgacaaag
      241 gaaaatttct catgacactg aaagcaagca agcaagcaag caagcaagca agcaagcaag
      301 caagcaagca agcaagcaag caagcaagca agcaagcggt cgggttaatc tattaacatt
      361 atctgtttta tcgctgtttt gcacgccata tgtttgaggt tcggatgcgt acgatcccgt
      421 caaagaagcc gagattaaaa acaaatttat tttagaagcg gcggaagaca gaaattccca
      481 cgtttggcgc ggcccgtgca gcatatcttt tgattgcttc ggtatgttca gagctcagct
      541 tggttcaaat actcgttcta ccaaaatcgg cgacgatgcc gatttttcat tttcagacaa
      601 gccgaaaccc ggcacttccc attatttttc cagcggtaaa accgatcaaa attcatccga
      661 atatgggtat gacgaaatca atatccaagg taaaaattac aatagcggca tcctcgccgt
      721 cgataatatg cccgttgtca aaaaatatat tacagagaag tatggggctg atttaaagca
      781 ggcggttaaa agtcaattac aggatttata caaaacaaga ccggaagctt gggcagaaaa
      841 taaaaaacgg actgaggagg cgtatatagc acagtttgga acaaaattta gtacgctcaa
      901 acagacgatg cccgatttaa ttaataaatt ggtagaagat tccgtactca ctcctcatag
      961 taatacatca cagactagtc tcaacaacat cttcaataaa aaattacacg tcaaaatcga
     1021 aaacaaatcc cacgtcgccg gacaggtgtt ggaactgacc aagatgacgc tgaaagattc
     1081 cctttgggaa ccgcgccgcc attccgacat ccatatgctg gaaacttccg ataatgcccg
     1141 catccgcctg aacacgaaag atgaaaaact gaccgtccat aaagcgtatc agggcggtgc
     1201 ggatttcctg ttcggctacg acgtgcggga gtcggacaaa cccgccctga cctttgaaga
     1261 aaaagtcagc ggacaatccg gcgtggtttt ggaacgccgg ccggaaaatc tgaaaacgct
     1321 cgacgggcgc aaactgattg cggcggaaaa ggcagactct aattcgtttg cgtttaaaca
     1381 aaattaccgg cagggactgt acgaattatt gctcaagcaa tgcgaaggcg gattttgctt
     1441 gggcgtgcag cgtttggcta tccccgaggc ggaagcggtt ttatatgccc aacaggctta
     1501 tgcggcaaat actttgtttg ggctgcgtgc cgccgacagg ggcgacgacg tgtatgccgc
     1561 cgatccgtcc cgtcaaaaat tgtggctgcg cttcatcggc ggtcggtcgc atcaaaatat
     1621 acggggcggc gcggctgcgg acgggcggcg caaaggcgtg caaatcggcg gcgaggtgtt
     1681 tgtacggcaa aatgaaggca gccgactggc aatcggcgtg atgggcggca gggccggcca
     1741 gcacgcatca gtcaacggca aaggcggtgc ggcaggcagt tatttgcatg gttatggcgg
     1801 gggtgtttat gctgcgtggc atcagttgcg cgataaacaa acgggtgcgt atttggacgg
     1861 ctggttgcaa taccaacgtt tcaaacatcg catcaatgat gaaaaccgtg cggaacgcta
     1921 caaaaccaaa ggttggacgg cttctgtcga aggcggctac aacgcgcttg tggcggaagg
     1981 cgttgtcgga aaaggcaata atgtgcggtt ttacctgcaa ccgcaggctc agtttaccta
     2041 cttgggcgta aacggcggct ttaccgacag cgaggggacg gcggtcggac tgctcggcag
     2101 cggtcagtgg caaagccgcg ccggcattcg ggcaaaaacc cgttttgctt tgcgtaatgg
     2161 tgtcaatctt cagccttttg ccgcttttaa tgttttgcac aggtcaaaat ctttcggcgt
     2221 ggaaatggac ggcgaaaaac agacgctggc aggcaggacg gcgctcgaag ggcggttcgg
     2281 cattgaagcc ggttggaaag gccatatgtc cgcacgcatc ggatacggca aaaggacgga
     2341 cggcgacaaa gaagccgcat tgtcgctcaa atggctgttt tgatgcgtcg ggaaatgttt
     2401 tgacgcacag gcggcacacc ggcacggccc cgcgcgccgc cccgcaaacc aatccgaacc
     2461 ctgccgcccc gaagggcggg gcatcatgat gaaaccggcg gaaaaccgcc ggttttttgc
     2521 cgccgtttga aacccgattc tggcttcaga cggcattgtc gcggcatcgg gcggcagggt
     2581 ttggaacagc ggcataaaaa actgatacaa tccgccgatt gataatggtt attttttatt
     2641 tttgtgggaa gacatttatg cctgcacgaa acagatggat gctgctgcct ttattggcaa
     2701 gcgcggcata tgccgaagaa acaccgcgcg aaccggattt gagaagccgt cccgagttca
     2761 ggcttcatga agcggaggtc aaaccaatcg acagggagaa ggtaccgggg caggtgcggg
     2821 aaaaaggaaa agttttgcag attgacggag aaaccctgct gaaaaatccc gaattgttgt
     2881 cccgcgcgat gtattccgca gtggtctcaa acaatattgc cggtatccgc gttattttgc
     2941 cgatttacct acaacaggcg cagcaggata agatgttggc actttatgca caagggattt
     3001 tggcgcaggc agacggtagg gtgaaggagg cgatttccca ttaccgggaa ttgattgccg
     3061 cccaacccga cgcgcccgcc gtccgtatgc gtttggcggc agcattgttt gaagacaggc
     3121 agaacgaggc ggcggcagac cagttcgacc gcctgaaggc ggaaaacctg ccgccgcagc
     3181 tgatggagca ggtcgagctg taccgcaagg cattgcgcga acgcgatgcg tggaaggtaa
     3241 acggcggctt cagcgttacc cgcgaacaca atatcaacca agccccgaaa cggcagcagt
     3301 acggcaaatg gactttcccg aaacaggtgg acggcacggc ggtcaattac cggctcggcg
     3361 cggagaaaaa atggtcgctg aaaaacggct ggtacacgac ggcgggcggc gacgtgtccg
     3421 gcagggttta tccggggaat aagaaattca acgatatgac ggcaggcgtt tccggcggca
     3481 tcggttttgc cgatcggcgc aaagatgccg ggctggcagt gttccacgaa cgccgcacct
     3541 acggcaacga cgcttattct tacaccaacg gcgcacgcct ttatttcaac cgttggcaaa
     3601 ccccgaaatg gcaaacgttg tcttcggcgg agtgggggcg tttgaagaat acgcgccggg
     3661 cgcgttccga caatacccat ttgcaaattt ccaattcgct ggtgttttac cggaatgcgc
     3721 gccaatattg gatgggcggt ttggattttt accgcgagcg caaccccgcc gaccgaggcg
     3781 acaattttaa tcgttacggc ctgcgctttg cctgggggca ggaatggggc ggcagcggcc
     3841 tgtcttcgct gttgcgcctc ggcgcggcga aacggcatta tgaaaaaccc ggctttttca
     3901 gcggttttaa aggggaaagg cgcagggata aagaattgaa cacatccttg agcctttggc
     3961 accgggcatt gcatttcaaa ggcatcacgc cgcgcctgac gctgtcgcac cgcgaaacgt
     4021 ggagtaacga tgtgttcaac gaatatgaga aaaatcgggc gtttgtcgag tttaataaaa
     4081 cgttctgatt gctgttcctt ttcggaggaa accctgccgg cggcggtatc acggcgggca
     4141 tcggcggctt tcgggcggtg ctttgcgtgc cgccgcgtgt gcggaaacgc attccggttt
     4201 ttccggcata acggcgatgc gaggtaaaat gccgtctgaa acccgattcg ggcttcagac
     4261 ggcattgtcg cggttgcggc aggcgggttc accagattcc gtcaaaggtt ttcgcgccgc
     4321 gccaaaattt ccacctgtcg gcgggtttga aggtcagcgt accgccgtgt tgtccgtccg
     4381 tggtgatgtc cagccgtttg attttgccgg tacggacggc ttcgtagatc ggtgcgaacc
     4441 agcgttcttc ccactgctgc aatattgccg cataccgctc cctgtcccct gtcagggcgg
     4501 tcaggcgcaa atcgtccata aacaggatat ggtgcgtgtc gggtaggtat gccgccgttt
     4561 cttcataggc gcggaagttg tcgggcaatg cgcggcggtc ggggtggaaa cggctccaaa
     4621 ccgtatcggc gaaaagcgtg ccgccttgac tgcttccgtg cagactgtcc caaagccaca
     4681 gcccgttcaa ctcgggcagc ccgcgtttct tgcggttatg gttgacgggg tgcgccgcca
     4741 gccacatttg gatttcggtt tggacgcgca gccattccaa cgcgtcttcc ccgtccggct
     4801 gatcgtccgc gcccaacaat ccgcccaagt ccaaaacggg cttcgcgccc cagcggtacg
     4861 cgcaaggaag ggaaaccagc cataattcgg gcaggacggg aacgaaacgc catggaatgt
     4921 cgccgtaaaa cgccgacagg tcgcggcaga gccgttccgc ttcatccgta ccgacgttca
     4981 gatattccgc cgttagcaca tttgcctgat gcatccccat cttttgccag acgggcgtgg
     5041 cgagcgcgac ggcttcagac ggcatattca ggctttgtgc cgcgcgttcc gccagtctgc
     5101 cgcaccacaa ataacgcgcg taaaatgccg aagccgtgca gctttggcgg tgcagcgagc
     5161 cgtattgcag gattttgttg aaggcgggca ggcagagagg tattcgggtt tcgtcttcat
     5221 ccaaattgag cgagggaatg gcgagggtga gtttcatcgt ttgacgtttc agaaatgcag
     5281 gtcaggcgca acattataga ggattcggcg caaacgccgt caaaaaggaa caatatggct
     5341 gtcttcccac tttcggcaaa acatcggaaa tacgcgctgc gcgcgcttgc cgtttcgatt
     5401 attttggtgt cggcggcata cattgcttcg acagagagga cggagcgcgt cagaccgcag
     5461 cgcgtggaac aaaaactgcc gccgctgtct tggggcggca gcggtgttca gacggcatat
     5521 tgggtgcagg aggcggtgca gccaggcgac tcgctggcgg acgtgctggc gcgttcgggt
     5581 atggcgcggg acgaaattgc ccgaataacg gaaaaatatg gcggcgaagc cgatttgcgg
     5641 catttgcgtg ccgaccagtc ggttcatgtt ttggtcggcg gcgacggcgg cgcgcgcgaa
     5701 gtgcagtttt ttaccgacga agacggcgag cgcaatctgg tcgctttgga aaaaaaaggc
     5761 ggcatatggc ggcggtcggc ttctgaggcg gatatgaagg ttttgccgac gctgcgttcg
     5821 gtcgtggtca aaacgtcggc gcgcggttcg ctggcgcggg cggaagtgcc cgtcgaaatc
     5881 cgcgaatcct taagcgggat tttcgccggc cgcttcagcc ttgacggttt gaaggaaggc
     5941 gatgccgtgc gcctgattta cgacagcctg tatttccacg ggcagcaggt ggcggcgggc
     6001 gatattttgg cggctgaagt cgttaagggc ggcacaaggc atcaggcgtt ctattaccgt
     6061 tcggacaagg aaggcggagg gggcggcaat tattacgatg aagacggcag ggtgttgcag
     6121 gaaaaaggcg gcttcaacat cgagccgctg gtctatacgc gcatttcttc gccgttcggc
     6181 taccgcatgc accccatcct gcacacttgg cggctgcaca cgggcatcga ttatgccgca
     6241 ccgcagggaa cgccggtcag ggcttccgcc gacggcgtga ttacctttaa aggccggaag
     6301 ggcggatacg gcaacgcggt gatgatacgc cacgccaacg gtgtggaaac gctgtacgcg
     6361 cacttgagcg cgttttcgca ggcacaaggc aatgtgcgcg gcggcgaggt catcggtttt
     6421 gtcggttcga cagggcgttc gaccgggccg cacctgcatt acgaggcacg catcaacggg
     6481 cagcccgtca atcctgtttc agtcgcattg ccgacacccg aattgacgca ggcggacaag
     6541 gcggcgtttg ccgcgcagaa acagaaggcg gacgcgctgc ttgcgcgctt gcgcggcata
     6601 ccggttaccg tgtcgcaatc ggattgaagt ttgaaccggc gacgaaaaca atgccgtctg
     6661 aaaatctgca aacaggtttt cagacggcat ttatagtgga ttaacaaaaa ccagtacgtc
     6721 gttgcctcgc cttagctcaa agagaacgat tctctaag
//
LOCUS       DAWKOD010000105         6742 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000105 DAWKOD010000000
VERSION     DAWKOD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.105"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(239..1045)
                     /locus_tag="WJK73_001524"
     CDS             complement(239..1045)
                     /locus_tag="WJK73_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7557990.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
     gene            complement(1087..1182)
                     /locus_tag="WJK73_001525"
     CDS             complement(1087..1182)
                     /locus_tag="WJK73_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ7557991.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            complement(1194..2033)
                     /locus_tag="WJK73_001526"
     CDS             complement(1194..2033)
                     /locus_tag="WJK73_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7557992.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDWMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            complement(2075..3120)
                     /locus_tag="WJK73_001527"
                     /pseudo
     CDS             complement(2075..3120)
                     /locus_tag="WJK73_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3137..5200)
                     /locus_tag="WJK73_001528"
     CDS             complement(3137..5200)
                     /locus_tag="WJK73_001528"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ7557993.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(5221..5631)
                     /locus_tag="WJK73_001529"
     CDS             complement(5221..5631)
                     /locus_tag="WJK73_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ7557994.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(5707..6612)
                     /gene="glyQ"
                     /locus_tag="WJK73_001530"
     CDS             complement(5707..6612)
                     /gene="glyQ"
                     /locus_tag="WJK73_001530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7557995.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
BASE COUNT         1423 a         1778 c         1858 g         1683 t
ORIGIN      
        1 caggcaggaa tctagaattt taatgcggca agaatttatc ggaaaaaacc gaagtttaaa
       61 gacctagatt cccgcctgcg cgagaatgac gagattttag gtttctgttt ttggctttct
      121 gttttctcgg gaatcacgaa ttagggctta cccgaaaaca agtgaaaccg aacaaaccgg
      181 attccccgac ataaatttag ggacagcgca cacccgccaa ataaggaaag gccaaaacct
      241 attcaaacgg gacaacgtgc cgcccgaggt gtttttccaa ttttttctga cgtttccgtt
      301 ccctcgccct ttcgatttcc ctgccgattt ttcccaaagc gcgtttcaac aaaaccttcg
      361 gggagcggcg gttgcggtac gattcctgcc tgccgttttt cagttggctg ggcagggttt
      421 cgctttggac gcacaaggcg ggggtcagtt ggcaaaccgt catcccccct ttgtcgagaa
      481 aactgctgaa gagcatccaa tccaccgcct tcaggcgttc agacggcaaa acggcaaacc
      541 tgtccaaaaa gaaacgcatc gcctttcggg aaataatata gcccgccata ccaaaatgtt
      601 cgctttccaa cagcggaaag gcgcgcccgc cgtagtccgc cacgccggag ggcgaggtca
      661 ggacgtgcat aaacatcgtt tccaagcgga cgataaaggc ggaatccttg tcaaaacgct
      721 cttgcagcca agcgtcttcg gcaaggaatt tctccgcacc ttcgccgagt aaaacgtcgt
      781 cctcaaatac ggcgatatac ggcagacctt catccaatgc ctgcttccac aatacggcgt
      841 ggctcataaa gcaggctttt tccactccgc tcaaataggg gtgcgccgac aaaccgggta
      901 cgagttccgc cattgcctgc tccagtgctt cagacggcat cagtgcgtcg aaaaactgaa
      961 acggaatgtc gtgcctgccg aaggtatcgg caatgtgcgc cctgcgttct gcagcggaag
     1021 ccaagctgat aacgtggttt tgcataattt atcctgtttt ttgtctgttg gataaagcgg
     1081 cgtttttcaa cggtttttca gcaatcggtg caaaatgccg aagtattgcc tcaaggtaaa
     1141 cagccgccgc atcctgccgt ctgccgcaaa tacgatgtcc atcttttctc cttttattgg
     1201 aaaggcacaa tgatcttgcc gattaactgt tcgcgccttt gccgacgttt ttccctttcc
     1261 ctgctgattt tggtcaaggc gcggatcagg cggtgtttga atgtgttggc gggggaatcg
     1321 cgcctttgct gtttgcggtt caggaggcgg tcgtgttcga tcaggctgcc caatgcgctg
     1381 ttttggtcgt gaaacttggc ataatgcagc tcttgggcgc acaaggcggg atcgagctgg
     1441 caaaccggca ttccttccct gtcgaaaaaa tcgctgaaca tcatccaatc gacggggtgc
     1501 agcccttcgg gcggcagggc ggcaaacctg tccaggaaaa accgcatcgc ttttcgggaa
     1561 atgatatagc ccgccgtccc ccagtgttcg ctttccaaca gcgggaaggc gcgcccgccg
     1621 tagtccgcca cgccggaggg cgaggtcagg acgtgcataa acatcgtttc caagcggacg
     1681 ataaaggcgg aatccttgtc aaaacgctct tgcagccaag cgtcttcggc aaggaatttc
     1741 tccgcgcctt cgccgagtaa aacgtcgtcc tcaaatacgg cgatatacgg cagaccttcg
     1801 tccaatgcct gtttccacaa tacggcgtgg ctcataaagc aggctttttc cactccgctc
     1861 aaataggggt gcgccgacaa gccggggacg agttccgcca ttgcctgttc cagcctttca
     1921 gacggcatca gtgcgtcgaa aaactgaaac gggatgccgc gcgcgccgaa ggttgcggca
     1981 atgtgcgccc tgcgttctgc ggcggaagct aagctgataa cgtggttttg cataatttat
     2041 cctgtttttt gtctgttgga taaagcggcg tttttcaacg gtttttcagc aatcggtgca
     2101 aaatgccgaa gtattgcctc aaggtaaaca gccgccgcat cctgccgtct gccgcaaaat
     2161 ccagccacgc gccggcgggc agcgtgtccg tccgtttgaa gcattggtac aaaaaccggc
     2221 gggcgcgttc aaaatcttct tccggcaaat gtttctccag caattcatac gctactgctt
     2281 ttatttggcg gtattcaagg ctgtcgaacc gggttttaaa acccatagac tgcaaaaaat
     2341 cgtttctggc ggttttttgg atgccctgcg cgatttcgtg ttggcggacg ctgtatttgg
     2401 atgaaacctg attggcgtga aggcggtatt tgaccaaggc ttcgggataa taagccagcc
     2461 tgcccaattt actgacatcg taccaaaatt ggtaatcttc cgcccaatcc cgctcggtgt
     2521 tgtaacgcaa accgccgtca atgacgctgc gccgcataat catcgtgttg ttgtgtatgg
     2581 ggttgccgaa agggaaaaag tcggcaatat cttcgtgccg ggtcggcttt ttccaaattt
     2641 tgccgtgcct gtgatgccgc gccagccggt tgccgtcctt ttcttccgac aacacttcca
     2701 gccacgcgcc catcgcgatg atgctgcggt ctttttccat ctcgcccacg attttctcaa
     2761 tccagtcggg ggcggcaata tcgtcggcat cggtgcgtgc aatatattcc ccccccccga
     2821 ctttgccaat tcgtccagcc cgatgtttaa agagggaatc aggccggaat tttgagcttg
     2881 tgcaaggatt ttgatacggc tgtcccgctt ttgaaaatcc ttggcaatgg caagcgtacc
     2941 gtctgtcgag ccgtcatcga caatcaaaat atccaagttg caccaagttt gattcacgac
     3001 ggcggctaat gattgggcga aatatttttc tacgttgtag gcgcaaatca atacgctgac
     3061 taaaggctgc aatttattct cccgataggc acgatgccgt ctgaaggctt cagacggcat
     3121 ttggactgta caacggttac tcgcccaaaa gcgcgatgtc ggctaccgcg ttcatttgct
     3181 ctgccaagcg gttcagcagg ttcaggcggt tttgttttac ggcggcatct tccgccatta
     3241 ccatcacgcc gtcaaagaat gcatcgactt gcggtttgac ggaagccagt tcggacaagg
     3301 cggtttggaa attgccttcg gcgacggcgg cggcgatttt cggctgcaag ccttgcgcgg
     3361 cggcaaagag ggctttttct tcgtcctgtt gcagcaggct ttcattgact tcgcccaact
     3421 cggcatcggc ttttttcagc aggttttgca cgcgtttgtt ggcggcggcg agcgcggcgg
     3481 cttcgggcag ctgtttgaac gcggcaacgg cttgcagttt ggcggtcaaa tcgtccaaac
     3541 ggcgcggctg tttcgccaat acggcggcaa cgatgtcttg cggataatcg ttttgcagca
     3601 acacggccaa acgcgcctgc ataaagtcgg cggtttcaga cggcgttttt tcgttgagca
     3661 aacctttggg gaagctgtcg aatgccgtct gaatcagttc gttcacgtcc aaaccatact
     3721 gcatcagcat acgcaaaata cccaaggcag cgcggcgcag ggcgtagggg tctttgtcgc
     3781 cggtcggaat cagaccgatg ccccaaatgc cgaccagggt ttccagcttg tcggcaagtg
     3841 caacggcagt ggcaattttg ctttcgggca gcttgtcgcc ggcaaaacgc ggttggtaat
     3901 gttgctcgac ggcttcggcg atttcttcgg tttcgccgtc caagcgggca tagtatttgc
     3961 ccatcgtgcc ttgcagttcg ggaaactcgc cgaccatttc ggttaccaag tcggctttgg
     4021 caagacgcgc ggcgcgttcg gcagcggcgg catccgcgcc caatgctttg gcgatgtggg
     4081 cggcaatgct ttgcaggcgt tcgatgcgtt cggcttgcga gccgattttg ttgtggtaca
     4141 ccacgctgga caatttcggc aggcggcttt ccaaagtcgc tttttggtct tgtttgtaga
     4201 agaactcggc atcagacagg cgcgcgcgca aaacgcgttc gttgcctcgg atgatgtgcg
     4261 acgggtcttc ggtttgcagg ttggaaacca gcaggaagcg gttcatcagc ttgccgtttt
     4321 ggtcgagcag cggaaagtat ttttggtttt gctgcatcgt taaaatcaag cattcctgcg
     4381 gcacggcgag gaaatgttcc tcaaaaccgg cttccaaaac aacaggccat tcgaccagcg
     4441 cggttacttc gtccaacaat gcttcatcgg cggcgacggc ggcgttcaga ctgcgcgcct
     4501 gcccttccaa tgccgtctga atcgcggctt tgcgctcggc aaacgaagcg acgactttgc
     4561 cttgcccgcg catttgttcg gcgtagctgt cggcgttttc aatggtgatt tcgccgttgg
     4621 acaagaagcg gtgtcccaag gtttgattgc tgctttgcag acccaaaacg ctgacgttta
     4681 cgatgtcgcc gccgtgcagc acaatcagcc cgtgaacagg gcgcacgaag gtaaatgtgc
     4741 tgctgcccca acgcatcact ttgggaatcg gcagtttctt aaccacttga ttgataatgt
     4801 cttccaaaag tccgcccagc ggcttgccgg tttggacgta ttcgtaggcg tacacatcct
     4861 gcttgccgtc gtggacgatg gtcaagtctt cgattttcgc gcctgcaccg cgtgcgaaac
     4921 cttccaaagc cttggtcggc gcaccgtctt ttacggcatt cgccacggca gggccttttt
     4981 tcacgatttt ttggtcggcc tgaacggctt tgacgttttt gacttgaacc gccaaacggc
     5041 gcggcgaggc ataagccgta aattcggctg caccgtcaac cagttgcgct ttttccaagc
     5101 cttcggcaac ggcggcggca aaatggttgc ccagattatt cagggctttg ggcgggagtt
     5161 cttcggtaag gagttcgatt aaaagggttt gggtcatcat tcggctttct ttgaatttgg
     5221 ttaatctgcc tgtttatagg tttcgctgta attttcccag ccgtcatccc cgtaaaaacc
     5281 atcaaccagc ggggtggcgt acaaagtggc gacatcttcg cggcctgcca gccaagagat
     5341 aatggctttt ttctcggttt cgcccaagct tcgggcaccg gatttttgaa acaggcacga
     5401 aaaatcgccg caatcgcccc cgcccgccat ttccaagcca tttgcctcaa gatacgcaat
     5461 cagctcgtcc ataaagcggt cgaacgcttc agcatcgtcc gcagcttggt gcaaactgcc
     5521 tcgaacgccg aaaatcaatg cttgaaactc gcccaaatgc agctttttat gctggcggcg
     5581 gttcattttg tgcaggcgtt tcctgctcgg ggtgcggaaa tagacaggca taattttcct
     5641 aaaaaatata atggcttccg gacggctgcc atatcgtgcc gcccgaacgt aaaaatcgtc
     5701 gctcccctag gcggcatttg ccttcattaa gggaaagccc agtttttcgc ggctttcgac
     5761 atatttttgc gccacggcgc ggctcaacgc gcggatgcgc ccgatgtagg tggcgcgttc
     5821 ggttacggaa atcgcgccgc gcgcgtccaa gaggttgaac gtatgccccg ctttgaggac
     5881 cagctcgtag gcaggcaggg cgagggcggc gttttcttcg gcaagcaggc gtttggcttg
     5941 cgcttcgtag tcgttgaact ggcgcagcag ccaatcggca tcgctgtatt cgaagttgta
     6001 ggtggattgt tcgacttcgt tttggtggta cacgtcgccg tagcttacgg tgttgccgtc
     6061 cggcgttttt gcccaaacga ggtcatagac gttttccacg ccttgcaggt acatcgccaa
     6121 gcgttcgatg ccgtaggtaa tttcgccgag tacaggcgtg cagtcaatgc cgccgacttg
     6181 ttggaaatag gtaaactggg ttacttccat gccgttgagc cagacttccc agcccaaacc
     6241 ccatgcaccg agggtggggt tttcccagtc gtcttcgaca aagcggatgt cgtggacttt
     6301 ggggtcgatg cccaattcgc gcagggagtc gagatagagg tcttggatat tggcgggagc
     6361 gggcttgagg gcgacttgga attggtaata gtgttgcagg cggttggggt tgtcgccgta
     6421 gcgaccgtct ttggggcggc ggctgggttg gacgtaggcg gcaaaccaag gctcgggccc
     6481 gagtgcgcgc aggcaggtgg cggggtggga agtacctgca ccgacttcca tgtcgaaggg
     6541 ttggataacg gtgcagcctt tgtctgccca gaatgtttgc agtttgaaga tgatttgttg
     6601 gaaggtaagc atggcttatg attcgataaa ataaagggtt tattttactg tttccattgc
     6661 tgtttggata ggtttatctc aaagacagac tgatttgaaa acacggcata catgatatag
     6721 tggattaaat ttaaaccagt ac
//
LOCUS       DAWKOD010000106         6720 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000106 DAWKOD010000000
VERSION     DAWKOD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.106"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(95..727)
                     /locus_tag="WJK73_001531"
     CDS             complement(95..727)
                     /locus_tag="WJK73_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7557996.1"
                     /translation="MEKIEKFKSELLNAIFQYTQCISIFVYKKNIYYLIDCKENFILN
                     MKLDLDLDFKNGNITLEQYQDEMNSYYYRNGIWQLTKDNFESYLQSDSVIELKKDELK
                     KLMFQGFTSAEAVRLYSVVENKLSYNDPISDSGQQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(882..1376)
                     /locus_tag="WJK73_001532"
     CDS             complement(882..1376)
                     /locus_tag="WJK73_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ7557997.1"
                     /translation="MIFKQNQNYWAVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTKR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLDAWDAIDADDVILSLDNSPEEIGTGLRLAL
                     SRCR"
     gene            1694..3127
                     /locus_tag="WJK73_001533"
     CDS             1694..3127
                     /locus_tag="WJK73_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ7557998.1"
                     /translation="MNEYSQLIKHPDISLSPISDSIGIGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            3236..4024
                     /locus_tag="WJK73_001534"
     CDS             3236..4024
                     /locus_tag="WJK73_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ7557999.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            4392..5948
                     /locus_tag="WJK73_001535"
     CDS             4392..5948
                     /locus_tag="WJK73_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7558000.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            5965..6711
                     /gene="surE"
                     /locus_tag="WJK73_001536"
     CDS             5965..6711
                     /gene="surE"
                     /locus_tag="WJK73_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ7558001.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQFPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         1659 a         1627 c         1750 g         1684 t
ORIGIN      
        1 agtgttgttt tacaggaaag taaaggccgt ctgaaagggt ttaccagctt tcagacggcc
       61 ttttgaattc atgcaagcta tggcttgctt atatttataa ttgtctaaat ttccaataat
      121 cccgtccttc aattttccaa taacaaaatt cgtccggtat taaataccca aaatccatcg
      181 cctttgaaaa ccagccatca taaacataat cttcatgaca tctgtcccaa tccatatgta
      241 agtacacttc agtgtcaaaa ttgacataga ataaaggcag cctcgaggaa atctgattga
      301 tttttaagaa atcactttgc tgtccagagt ctgagatagg atcattgtac gacagcttgt
      361 tctcgacaac ggaatataac cttaccgctt cagcagaagt aaatccctga aacatcagtt
      421 tcttcaattc atcctttttt aattcaatga ctgaatctga ttgaagatag ctttcaaaat
      481 tgtccttagt taactgccag atgccgtttc ggtaataata actattcatt tcatcctgat
      541 attgttctaa cgtaatatta ccgtttttaa aatctaaatc taagtctaat ttcatgttta
      601 atatgaaatt ttccttacaa tcaatcaaat aatatatatt tttcttatag acaaaaatag
      661 atatgcattg agtatattga aagatggcat ttaataattc agatttaaat ttctcaattt
      721 tttccatatt tgccaaattc cttaatagct ttatcttcag caagtcgtag cccgcattaa
      781 atctgcaggt aaggtgtggt acgcacgcca gtgagcttga gtctaatgaa aaaggtcgtc
      841 tgaaacattt ttccggcttt cagacgacct tttgtcaaat attatcggca gcggctcaat
      901 gctaacctta aacctgttcc gatttcttca ggactgttat ccaatgataa aattacatcg
      961 tctgcatcaa tggcatccca cgcgtccagc ttgacatggc ggctcgggct gattttcagg
     1021 cagccgttgt gcagccaaat atccacgctc atcatgtttt taaatagggc gcgtttggtt
     1081 ttatagccca agttcccgca tagcttggca acccaatcct catagcgttg ccgaattttt
     1141 tcggtatcaa aaaaatcttg gtcttctgga ctgtcataaa cgaaagtcct gctgtttgcc
     1201 aatgcttgca agaccgttgt gcctaaagtt tcattgtcgg tatccaatgg caggatatga
     1261 gggggatata ggtggtctat tgccgttaat cccaaacctg aacatgtttg aacaatcaga
     1321 gcttcttcat tcgtataaaa aactgcccaa taattttgat tctgtttaaa aatcattatt
     1381 ttatctccgt aattttgact gtaatgtttt gacttttgcc atactctacc acacgttgca
     1441 actgcaatct ttgctcctta tatattgcac cccatcaaag ggctgcatta cttttctttc
     1501 taaagattac cattgtataa aaaagtattt ttcttaaacg acagggattg cgtccgcgcc
     1561 atattcagac actgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttag
     1621 aatatgaaga tgataatgtc cgtgagattg acggcatttg aaagcggtta tcccgcatac
     1681 cggaggacac gaaatgaacg aatattccca attaatcaag catcccgata tttccctttc
     1741 cccgatttca gacagcatcg ggatcggcaa tccggcgacg ggtgaaattt tggcgtatgt
     1801 ccgcaacacg ggttcggaca aactgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa
     1861 attatgggcg gcgaaaactg ctttggaacg cgccgacata ttgtggcgtt ggtatttttt
     1921 agttaaagaa aacaaggaag cactggcgcg cctgatgacg atggagcagg gcaaaagcct
     1981 gaccgagtcg cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgcggaaga
     2041 ggcgcggcgg attgacggcg atgtgctgac aagtgtgaaa gcgtcgcaaa aactggtcgt
     2101 gttgaaacag cctgtcggcg ttaccgccgc gattacgccg tggaacttcc catctgcaat
     2161 gattgcgcgc aaggctgcgc ctgctttggc ggtgggctgc gcgatgatcg tcaaacccgc
     2221 atcgctcacg cctttgagtg cgtatgcgtt ggtcgtgctg gcttatgaag cgggcgtacc
     2281 acaggatttg ttgcccgtcg tcagcggtcg cgcatcggaa atcggccgtg aatttgccac
     2341 gaacccgatt atccgcaaaa tcagcttcac cggctctacc gaagtcggtg cgaaaatttt
     2401 tgccgacagc gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt
     2461 tatcgtgttt gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt
     2521 ccgcaacagc ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat ccggcattta
     2581 cgacgaattt tgtcgcaaat tgagtgaaaa agtagccgag ctcaaattgg gcaacggctt
     2641 ggaggatggc gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca
     2701 gcacattgct gacgcgctcg ctaaaggcgc ggtctgtctg accggcggca aacgcagcgc
     2761 attgggcgga acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt
     2821 ggctcgcgaa gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga
     2881 ggtcatcgag gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga
     2941 caccgcccgc caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac
     3001 gggcttaatc agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg
     3061 tgaaggcagc aaatacggtg cggacgaata tctggaattg aaatatttgt gtatggatgt
     3121 cgggtgacgg atgccgtctg aacggcaggg tttcagacgg cgccgcattt taagcagtct
     3181 ctatctgttg tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat
     3241 ggaaaacgga aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcatct
     3301 cttaatcagc ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc
     3361 ttatccttat gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga
     3421 tattgtatgt ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc
     3481 acgcatggtc gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct
     3541 gcacagcgtt tcgctggcgc gtcctgtggt ggaagcgttt gaacaggatc gtatgaccat
     3601 tgttacggcg gcggaagtcg tggtcgaaga tttgcacaaa gcccccgaag ggctgcaaag
     3661 cctgtcgtgg ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa
     3721 gaacaagacg ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccggtg
     3781 gctgccgtat tccgacaagg aagcagaaaa aatccgcaaa catctgaaac cgctgaaagt
     3841 cttggcggat gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga
     3901 aggggaggag ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat
     3961 taccgataaa gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc
     4021 ttaagcgttg ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac
     4081 tgtatttgag aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg
     4141 ccgtccgtgt gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg
     4201 gtatggcatt ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa
     4261 ggtacacgca catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg
     4321 taccgtctgt tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga
     4381 atacgaaaat catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca
     4441 cgcttttggt gttggaagtc gtattgggga tagacaacct tgtctttgtg gcgattttgg
     4501 caaacaaggt ccagcccgca cggcgcgacc gagcacggat tatcgggctg gggctggcag
     4561 tcgtcatccg catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc
     4621 tgttccaaat cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta
     4681 ttttcctgct ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt
     4741 ttaccgttgc cgacaaccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc
     4801 tgatactgga tgccgtgttt tccatcgatt cggtcattac tgcggtggcg atggtcgatc
     4861 atatcgtcgt ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca
     4921 aactcttgac cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt
     4981 tgttgatgat cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct
     5041 acctctacgc cgccatcggc ttctcgattt taatcgaact gtttaaccag atttcccagc
     5101 gcaacagccg caaaaacgac tacatcggca gctcgtggcg caaacggact gctgaaaacg
     5161 ttttgggtat gatgggcata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg
     5221 acgacgcgca ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg
     5281 aacgcccgat tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc
     5341 aaagccgcga agaacagtat gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg
     5401 tcggaaaggc gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc
     5461 aactgctgga aacaggcggt ctcgacattc agacggcatt gcgccagccg ctcgttttgc
     5521 ccgacagcac taccgcgctg ggcgcgctcg aactcttccg ccaaagcagc gcggattatg
     5581 ctttggtggt ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg
     5641 aaaccatcgc aggcgagttc cccgaagaat ttgagcgcga agaagaacca gccgttcagg
     5701 ggaatcccga tgaaagcctg acggtggaag gcgcgttgga atatgtggag ctggcatccc
     5761 aactcaatct gccgcagcag gaggaagatg ccgatttcca tacggttgcc gggctgatta
     5821 tggaagaatt gcaaaccatc cccgatgtcg gcgattttgc cgatttccac ggctggcggt
     5881 ttgaagtggt cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg
     5941 aagaataagc attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc
     6001 tccgaaggta ttgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtt
     6061 gcgcccgaac gcgacaggag cggggtcagc aattcgctga cgctggaacg ccctttgcag
     6121 ttgaaacagg cgcaaaacgg gttctactat gtcaacggca cgccgaccga ctgcatccac
     6181 atcgggcagt ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg
     6241 ggcgcgaata tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc
     6301 taccttatgg gcatacccgc tgtggcgttt tccttaaacg acgcttccgg acgctattgg
     6361 gcaaccgcag aaaaggcact gtggacattg ttggcgcatt tttttaaaac ccccccgcag
     6421 ttccctattt tgtggaacat caatatcccc gccgtcgcgc cggaagatgt gcggggcatt
     6481 aaaatcgccc gtttgggcag gaggcatcac ggtcagaacg tcatccccgc gcgcaatccg
     6541 cgcggcgaac agatttattg gataggcccg gtaggcaggg tttccgacaa agaagaggga
     6601 acggatttcg gtgaatgcgg cgcaggtttc attaccgtaa cgccgctgca aatcgacctg
     6661 accgcctatc cggacatggc ggaaacagcg gtgttctggc atgcggactg accgtttcat
//
LOCUS       DAWKOD010000107         6577 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000107 DAWKOD010000000
VERSION     DAWKOD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.107"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..558)
                     /locus_tag="WJK73_001537"
     CDS             complement(<1..558)
                     /locus_tag="WJK73_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7558002.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHSVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAH"
     gene            797..1696
                     /gene="crgA"
                     /locus_tag="WJK73_001538"
     CDS             797..1696
                     /gene="crgA"
                     /locus_tag="WJK73_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ7558003.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1923..5138)
                     /gene="carB"
                     /locus_tag="WJK73_001539"
     CDS             complement(1923..5138)
                     /gene="carB"
                     /locus_tag="WJK73_001539"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ7558004.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(5145..5693)
                     /locus_tag="WJK73_001540"
     CDS             complement(5145..5693)
                     /locus_tag="WJK73_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558005.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5696..6259)
                     /locus_tag="WJK73_001541"
     CDS             complement(5696..6259)
                     /locus_tag="WJK73_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7558006.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
BASE COUNT         1538 a         1651 c         1613 g         1775 t
ORIGIN      
        1 gtgtgcctga taatctgcca agtatttttc cacttgcgga tttttaacca catcgttaca
       61 taagaatgtc ggcaggcggg tcatacccaa aaactcgttg gctttgtgga agtgcatata
      121 caaaacatca acgcctttgc cttcaaagaa atcgccttcg cgggtaaacg cctcaatcgg
      181 cgcattccaa gtcagtgaaa gcatatgttt tttgccttgc aacaagccgc ctgtgccgta
      241 gccctcagtc ggattgacgc tgtgtctgcc gtcgctttgg tagagtttgc cgtgtccagc
      301 ggttaatact tcgtctatgt attttttcac tgtccaaggc tcgtgcatcc accagcccgg
      361 catctgccaa atcacagcat ccatccaaac gaatttttcg atttctgcct caacatcata
      421 gccggcatca atcacggttt cttgaacatt gtgtccgagc gcggtcaaaa cttctttcgc
      481 ttttttgtga agcgtgcggt ttaacccgcc gtgagaatgt ccgaacgctt tgccgccgtc
      541 taataataaa atattcattt gttacctcgt ttgtgaattg atgaatgtat tgtgccgttt
      601 tactttataa tttaaaagtg caaaaataag aaaacacttt tgcgtcaaat gaaataatca
      661 gatgttccaa cacacgggat ggcacataaa gcaccgccct gtgtgccgtc ctgattcgga
      721 aggggttaca cccctcccaa ataaagtctg atcctgccgc cctaaagggc ggggttccaa
      781 ccgaaaagga aacacgatga aaaccaattc agaagaactg accgtatttg ttcaagtggt
      841 ggaaagcggc agcttcagcc gtgcggcgga gcagttggcg atggcaaatt ctgccgtaag
      901 ccgcatcgtc aaacggctgg aggaaaagtt gggtgtgaac ctgctcaacc gcaccacgcg
      961 gcaactcagt ctgacggaag aaggcgcgca atatttccgc cgcgcgcaga gaatcctgca
     1021 agaaatggca gcggcggaaa ccgaaatgct ggcagtgcac gaaataccgc aaggcgtgtt
     1081 gcgcgtggat tccgcgatgc cgatggtgct gcatctgctg gcgccgctgg caacaaaatt
     1141 caacgaacgc tatccgcata tccgactttc gctcgtttct tccgaaggct atatcaatct
     1201 gattgaacgc aaagtcgata ttgccttacg ggccggagaa ttggacgatt ccgggctgcg
     1261 tgcacgccat ctgtttgaca gccgcttccg cgtaatcgcc agtcctgaat acctggcaaa
     1321 acacggcacg ccgcaatcta cagaagagct tgccggccac caatgtttag gcttcaccga
     1381 acccggttct ctaaatacat gggcggtttt agatgcgcag ggaaatccct ataagatttc
     1441 accgcacttt accgccagca gcggtgaaat cttacgctcg ttgtgccttt caggttgcgg
     1501 tattgcttgc ttatcagatt ttttggttga caacgacatc gctgaaggaa agttaattcc
     1561 cctgctcgcc gaacaaacct ccgataaaac acaccccttt aatgctgttt attacagcga
     1621 taaagccgtc aatctccgct tacgcgtatt tttggatttt ttagtggagg aactgggaaa
     1681 caatctctgt ggataattct ataaaaacct gccataaaat tgtttttact cacttaataa
     1741 ctttgagaaa aaataaataa gatgatgtat agtcggcggc aatggcagtc tatatgggga
     1801 gcgtgatagg gctgttcctt atgtacggct tgtgtgtggc aacatcggct ttccaaatga
     1861 caaaatgccg tctgacaccg taaaaaaagg tttcagacgg cattttcaac ccgattcagg
     1921 catcagctgc ggtttttcaa acgcccgtgc agttcttgaa cgctgtacac gcccaagtgg
     1981 tcgcggcttt tcgcgccttc gctcatcgct tcgccgcccg cagtagtggt gtattgcggc
     2041 acacgttgct gcaatgcgct ttggcggatg atgtggctgt cggacacgga ttgcggatcg
     2101 ctggaaacgg tattcacgac cagtgcgatt tcgccgtttt tcagcgcgtc gccgatgtgc
     2161 gggcggcctt cggggacttt attgatggtt tgtacaatca gcccgtgttc ggtcaggtat
     2221 tgcgccgtgc cgcgcgtggc gcagatgccg tagcctaaaa cttggaagtt tttagcggtt
     2281 ttaatgacgc gttctttgtc ttcttcgcgc acggagagga agattttgcc ggtcgggttg
     2341 aggcgttcgc ctgcgccgag ttgggctttg tagtaggctt cgccgaagct tgcgcccacg
     2401 cccatcactt cgccggtgga gcgcatttcc ggtcccaaaa tcgtatccac gcccgggaat
     2461 ttgatgaagg ggaacacggc ctctttaacg gcatagaaat cggggacgac ttctttttcc
     2521 acgccttgtt ctttcaggga aatgcctgcc atgcagcgcg cgccgacttt ggcgagcggc
     2581 acgccggtgg ctttggagac gaagggcacg gtgcggctgg cgcgcgggtt cacttccaat
     2641 acgaacacca cgccgtcttg tacggcaaac tgcacgttca tcagtccgac cacgcccagc
     2701 gcgtacgcca tcgctttggt ttggcggcgg atttcgtctt ggatttcttc gcttaaggag
     2761 tagggcggca gcgagcagcc ggagtcgccg gagtggatgc ccgcctgttc gacgtgctgc
     2821 atgatgccgc cgataaccac gtctttgccg tctgaaacgc agtccacatc gacttcaatc
     2881 gcgttgttga ggaagaaatc gagcaacacg gggctgtctt cggaaacctg cacggcttcg
     2941 cgcatgtatt tttgcaactc ttcggcagag tgaacaatct gcatcgcgcg tccgccgagg
     3001 acgtaagacg ggcgcacgac cagcggataa ccgatttctt cggctttgac gagggcttct
     3061 tcttcgttgt gggcgatgcg gttgggcggt tggcgcaggc ctaagtcgtt caacactttt
     3121 tggaagcgtt cgcggtcttc ggcggcatcg atgctgtcgg cggacgtgcc gatgatgttt
     3181 acgccgtttt caaccaacgc gttggcgagt ttcagcgggg tttgaccgcc gtaatgcaca
     3241 atcacgcccc acgggttttc ggtgcggacg atttccaaca cgtcttccaa tgtcagcggc
     3301 tcgaaataga ggcggtcgct ggtgtcgaag tcggtggaca cggtttctgg gttgcagttc
     3361 accataatcg tttcaaagcc cgattcgcgc agggcgagtg cggcgtgaac gcagcagtag
     3421 tcaaactcga tgccctgacc gatgcggttc gggccgccgc cgagaatcat cactttttta
     3481 cggtcggaag gacgggattc gcattcttct tcgtaagtgg agtaaagata ggcggtttcg
     3541 gtggcgaact cggcggcgca ggtgtcaacg cgtttgtaaa ccggatgcag cttcagcgcg
     3601 tagcggtgtt cgcgcacttc tttttcgctt acgttcaaca attgtgccaa acgtttgtcg
     3661 gagaagcctt tgcgtttcag acggcgtagg gcggcgaaat ccaaatcact caaaatgccg
     3721 tctgaaaccg ctttttcttc cttcatcaag tcttcgattt gcgccaggaa ccaagggtcg
     3781 atggcgcaga tttcgtggat ttcttccagc gtgaagccgg cgcggaacgc gtctgccaca
     3841 aacagcatac gttcggggcc ggggtttgcc agttcgcggc ggatttccgc tttgtcggag
     3901 cttctcggat tgaagccgca caagcctgtt tccaagccgc gcagggcttt ttggaagctt
     3961 tcctgaatcg tgcggcccat cgccatcact tcgcccacgg atttcatctg cgtggtcagg
     4021 cggtcgtctg cggcagggaa tttttcaaac gcaaaacgcg ggattttggt taccacatag
     4081 tcgatggacg gctcgaatga cgcgggggtt ttgccgccgg tgatgtcgtt gcgcaactcg
     4141 tccagcttaa agccgaccgc cagtttcgcc gccaccttcg caatcgggaa gcccgtcgct
     4201 ttggaagcca gcgcggacga acggctcacg cgcgggttca tctcaatcac aatcatctcg
     4261 ccgttttcag ggttcaccgc aaactgcacg ttcgagccgc cggtatccac gccgatttcg
     4321 cgcaatactg ccaacgaagc attacgcatg atttggtatt ctttgtccgt cagcgtttgc
     4381 gcaggcgcaa ccgtaatcga gtcgcccgta tgcacgccca tcgggtcgaa gttttcaatc
     4441 gagcagataa tgatgcagtt gtcgttcttg tcgcgcacca cttccatctc gtactctttc
     4501 cagccgagaa cagactgctc aatcagcagc tcgtgcgtag gcgacgcatc gaaaccgcgt
     4561 tcgcaaatcg ccaaaaactc atctttattg taggcaatgc cgccgcccga accgcccatc
     4621 gtgaaagacg gacgaatcag ggtagggaag ccgacctgtt cttgcgctgc caaagcttcg
     4681 ttcatcgtgt ggcagacaaa agatttcggg caggagaggc cgattttttc catcgcttct
     4741 ttaaaacgac cgcggtcttc cgccttgtca atcgcgtctt ccgtcgcgcc gatcagctcg
     4801 acattgtatt tcgccagcac gccgttgcgc gccaaatcca gcgcacagtt cagcgcggtc
     4861 tgaccgccca tcgtgggcag aatcgcatca ggacgctcct tggcgataat cttctccacc
     4921 gtctgccaca taattggctc gatgtaggta acatccgcca tttcagggtc ggtcatgatg
     4981 gtggcggggt tggaattcac cagaatgact ttatagcctt cttcacgcaa agccttacag
     5041 gcctgtgcgc ccgaatagtc aaattcgcag gcctgaccga taacgatagg gccggcgccg
     5101 atgataagga tggattttag gtcggtacgt ttgggcatgg gtggttactc ttgaattaaa
     5161 gaaggttgtt ttgttacata gcttttaaga aaagtatttc ccttttctaa aattgaagct
     5221 aaaacatcat cttctatgtt agataaaata ttctctaaat ctgcgcttga tttaatttta
     5281 aagaattctt ttgtttgaga atggtataca agagaaggtt tatttagctg taaaagttct
     5341 gctatattat ttaatgaacc aatactctta ccatcccata aaataaagcc ataatcagca
     5401 atttctgcca ttttcttatc ttttgctgta taaaataccc ttccagttcc tttgctgtct
     5461 acttggacaa actgccaatt ccccacatta tttcggtaaa ttttgccaga aaagtaaata
     5521 tgcacattag cataatcttg ttcttgtaga aatttttgga tagctttatc agccccatta
     5581 gcatctccaa taacaatatc aaaattattg ctaagaatat tgttaattct ttctcgtatt
     5641 tgtggattta gtcgtgaaat gctgcgtgaa ccagaaaaga aaatagtgcg catttttatc
     5701 ctctagtctt tgtcattgct aaaacaatta cagatctagc attttttgaa aacaattttt
     5761 cagttgatat ttccaatgtt gctccgctat caaataaatc atcaattact aaaatattct
     5821 tattggatag atctatattg tcaattgtga tggaattatt cagtatttct agtttttctg
     5881 atttttcttc aatatttttg agtggtgtgt gtgagctaga ttttcttaaa ataggagaat
     5941 atggaatatt caagcgatca cttagttctt tggcaattaa ttgaactggt tgattaatac
     6001 gctctgttgt aaaaggggcg ggaacaatta aatttataga ttctaaacca ctaaattttt
     6061 gtaaaatata gtctaccagc aaagaaacat tttgggtttg attacgatat tttaattgat
     6121 aaacccattc tccaatcaca cttctcttgc tatcaaagtg aggatgccct aattcatcat
     6181 aacctaacaa aatacttttt tgcatatgat ggtctaaagc aaatcctttt gtccagttac
     6241 catttagttc tatcgccatt tcattgactc cttgtcaaaa ttaagtataa cgagcaatcc
     6301 tagcaagccg tagcccgcat gtagggtatg tgcggtacgc acgcacgcgt tcttcatttt
     6361 ccctgtaacc ccaacccacc gcatgcgcgc cttgcggcac acactctgcc gacggattca
     6421 aaggtcgtct gaaaaaacgc tcgcggcgtt tgttacatct gccttgcgat aaaggctgcc
     6481 tgaaaaattc ccactgttga tatttcgggc attctttctc aaaccgcctg caaccgcaaa
     6541 atccgctgca acctgccccc tcccccgtgg gggaggg
//
LOCUS       DAWKOD010000108         6532 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000108 DAWKOD010000000
VERSION     DAWKOD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.108"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..962)
                     /gene="truB"
                     /locus_tag="WJK73_001542"
     CDS             complement(42..962)
                     /gene="truB"
                     /locus_tag="WJK73_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ7558007.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1020..1544)
                     /locus_tag="WJK73_001543"
     CDS             complement(1020..1544)
                     /locus_tag="WJK73_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558008.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(1555..1926)
                     /gene="rbfA"
                     /locus_tag="WJK73_001544"
     CDS             complement(1555..1926)
                     /gene="rbfA"
                     /locus_tag="WJK73_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ7558009.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            2117..3361
                     /gene="clpX"
                     /locus_tag="WJK73_001545"
     CDS             2117..3361
                     /gene="clpX"
                     /locus_tag="WJK73_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ7558010.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3426..4619)
                     /locus_tag="WJK73_001546"
     CDS             complement(3426..4619)
                     /locus_tag="WJK73_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ7558011.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            5023..5523
                     /locus_tag="WJK73_001547"
     CDS             5023..5523
                     /locus_tag="WJK73_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558012.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5608..6162
                     /locus_tag="WJK73_001548"
     CDS             5608..6162
                     /locus_tag="WJK73_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558013.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1576 a         1710 c         1730 g         1516 t
ORIGIN      
        1 caacacatta ttcaaacaca gcccgtattt ttaaccgtcg ttcaggcggc ggatgccgcc
       61 gtgttcatca ggcgcaaggc tttcagacgg catatttcct tttgatattc cgctagaccg
      121 acaaagcggc cgttttccgt gtaaacgcgt accggcgtat cggaaggtag gtcttcctcg
      181 aaacgcggcc gctgcccgca gcggagcata tggacggcat aatcgtttaa aacggtttgg
      241 ggaaaatggg caaccaatac gtcgcagggc agcagcaggc tgtcgcgttc tgtttcgtcc
      301 aagtttgcca aggcttcgag cgtgtggctt tgggcgatgg taaagccggc ggtttcggtg
      361 cggcgcaggg cggtcaggtg ggcgaacgtg ccgatgtgtt tggcgatgtc ttcgctgagg
      421 gtgcggatat aggtgccttt gctgcaacgt acgtcgatga cggctttggg cgcgtcaaat
      481 tcggcaatat cgatggcgta aacggtaatg tcgcgcgcct tgcgttcgat gacgatgcct
      541 ttgcgggcgt attcgtacag cggtttacct tcgtgcttga gcgcggaaaa catcggcggc
      601 acttggcgga tgttgcctgt cagtgcttgg caggccgtct gaaattcggc taaggaaata
      661 tcggcgcggg cggtggcaac gatttcgcct tcggcatcgc ccgtgctgct ggcttcgccg
      721 agtttcagtg tggcggtgta ggctttgtcg gcattaatca gatattgggc gaacttggtc
      781 gcttcgccga agcagacggg caaaagcccg gttgccaaag gatcgagcac gccggtatgt
      841 ccggcttttt cggcgcggaa caagtgcctc gttttttgca gggcggtgtt gctggaaagg
      901 ccttcgggtt tgtcgagaag aagaacgccg ttgaccgggc gtttggcggg tttattggtc
      961 atattaatta gggaatatta atgttcagac cgtctgaaag ggttcggacg gcctgcgtgt
     1021 cagcttaatt cttcattcaa taattcacga acagagtgtt tttcatcaat tagatcaacg
     1081 ctgattgcat caaagccatt tcgttttaat gagtcaaaac taatgattcg accatcaatt
     1141 aaggggactt gtccttcaga attgacgaaa tcttcatatt catcttcata gtctatattg
     1201 taataaagat ggatcagaac tcggtaagta tactcatcca aatcagttaa cgttctgttt
     1261 tgcttttgat ttggactata tttgtcgcaa aacatagcgt tatagccatg ttccgcgcca
     1321 tcaaacaata cgatttcatc gccgcttaca gcttctactg ccacaatttt taccggagcc
     1381 ttatcagtat cggtcatcaa tccatcaaaa ggctctccat agtaacggat gtggaaagta
     1441 tctctacctt ggcaggataa tgaaaagcaa cgatgcacgc cttcaatttg caggctgtca
     1501 ctatgtgcat aggcggctaa atgttcggga atcagattgt tcattttagc agtatcagtc
     1561 ttcaaccggt ttttccgccg ccacttgatc gataagggcg gacaggttca aaccgcgttc
     1621 caacgattcg tcgtatttga aatgcagctc ggggattttg aacagcttga tgcgtttggc
     1681 caattcgctg cggaggtgtc cgcgtgcgtg ttccagcact tcttccgtaa tttcgcgcgc
     1741 atcttggttt aaaacggtgt agaacacggt ggcgtggctg taatcgcggg taacttcgac
     1801 ctcgttgacg gtgatgaagc cggcgcgggg gtctttcagt ccggtacgga cgagttcggc
     1861 aagctcgcgc atgatttgtt ctttgacacg gtcttggcgg gcgtagccgc gttggggttt
     1921 tctcatgaat atcctcagat ttcagatttg gtttcagacg gcatttttgc cgccggggcg
     1981 gttgcgaaag ggcgtattat agcctaatcg gcgggatttt aaggtaatac attgataaga
     2041 acggatatag ttattatact tggcaacccg aacggcacgt gccgttttct gtaacgcata
     2101 cattgtaaag aaagccatgt ccaacgaaaa ccgtacctgt tccttttgcg gaaaatccaa
     2161 atcacacgtc aaacatttaa ttgagggcga aaacgccttt atctgtgacg aatgcgtctc
     2221 aaactgtatt gaaatattgc atgaagacgg aaatgacggc acgccttcgg aaagtgccgg
     2281 aggggagccg gaagaatccg gcaagctgcc cacgcccgcc gaaatcgtcg ccaacctcaa
     2341 cgaccacgtt atcggtcagg aacaagccaa gaaagccttg gcggtgtcgg tgtacaacca
     2401 ttacaaacgc ctgcgccacc cgaaagccgg agctaatgtc gaattgtcga aatccaacat
     2461 cctgcttatc ggcccgaccg gatcgggtaa aaccctgctg gcgcaatctt tggcgcgcaa
     2521 actggatgtg ccgttcgtga tggcagatgc aaccacgctg accgaagcag gttatgtcgg
     2581 cgaagatgtc gaacagatta ttaccaaact gttgggcaaa tgtgatttcg atgtcgaaaa
     2641 agcccagcgc ggcattgtct atatcgacga aatcgataaa atttcacgca aaagcgacaa
     2701 cccgtcgatt acccgcgacg tatctggcga aggtgtgcaa caagccttgt tgaaactgat
     2761 tgaaggtaca gtggccagcg ttccgcccca aggcggccgc aagcatccga atcaggaatt
     2821 tatcaacgtc gataccacca acatcctgtt tatctgcggc ggcgcgtttg caggcttgga
     2881 aaaagtcatc cgccagcgta ctgaaaaagg cggtatcggt ttcggtgcgt ccgttcacag
     2941 caaggacgaa aatgccgaca ttaccaagct gttcggcatc gtcgaaccgg aagacttgat
     3001 taaattcggt ctgattccgg aattaatcgg acgtttgccc gtgattgcaa cactagaaga
     3061 actggatgag gacgcgctga ttaatatttt gaccgagccg aaaaatgctt tggtcaagca
     3121 gtatcaagcc ttgttcggta tggaaaatgt cgagttggaa tttgaagaag gcgcattgcg
     3181 ttccatcgcg cgtcaggcaa tggagcgcaa aaccggcgcg cgcggcctga ggtccatcgt
     3241 cgaacgctgc ctgctcgata cgatgtaccg cctgcctgat ttgcaaggct tgaaaaaagt
     3301 ggtggtcggt aaggcagtca tcgaagaggg cagggaaccg gaattggtgt tcgagtcctg
     3361 atggcaaaca aaaccgcttc ggaacgttga accacgtttc cgaagcggtt ttttgttttt
     3421 gatgcctagg ggcggtttgc ggcggcaaat tccgtcagca cgtgttccaa acgccgcaaa
     3481 ccttccgccg tatcctcatc gttcaacagc agcgaaggcg cgaaacgcaa tacgtccgca
     3541 cccgcaacca aaatcatcac gccgtgtttc aaggcggcgg cggttatttc ggatgcgcgt
     3601 ccgcgataag gcgcgtccaa cacgcagccg agcagcagtc ccatcccgcg aacctgtgag
     3661 aacaagcctg ttttcctgcc caaatccagc aatgccgtct gaagtttctg cccctggctg
     3721 cggacgttgt gcaaggtttc cggcgcgttg atgatgtcga aggcgcggct gccgaccgcg
     3781 cacgccatcg ggttgccgcc gaaagtcgag ccatgcgtgc ccggttggaa ggcggcggca
     3841 atcttttctg tcgccagcat cgcgccgatc ggaaagccgc agcccaaggc ttttgccgaa
     3901 ctcaaaatat cgggaacaat gccgtaatgt tcataggcaa acagcctgcc cgtatgcccc
     3961 atcccggttt gaacttcgtc caaaatcaac aacgcattgt gccggtcgca cagacggcgc
     4021 gcggtttgca aatattccgc agtggcgggc aggatgccgc tttcgccctg tatcggctcg
     4081 atgatgaccg cgcaggtctg ttcgccgacg gcagcttcca gcgcggcaat atcgttgaac
     4141 ggaacgtgcg taatgccttg cggcaggggt gcataatcct tgctgtattt cggctggccg
     4201 ccgacggaca cggtaaacag cgtgcgtccg tggaaactgt tgatacaggc gacgatttcg
     4261 ctttttcctc cgccgaaacg gtcgcgcgca tatttccttg ccagcttcaa cgccgcctca
     4321 ttcgcttccg cgcccgagtt gcagaaaaaa accttgtcgg caaaactgtt tgcaaccaat
     4381 ttttgcgcca attcctgcgc cggacgcgtc gtatagatat tggaaatgtg ccacagcttg
     4441 tgcatctgcg cgtttaaagc atcgacaagg gcagggtggc agtgtcccag cgcattgacg
     4501 gcgataccgc ctgaaaaatc aatatattca cgcccttccg tatcccaaac gcggctgcct
     4561 gaagcgcgtt cgggaatcat cggggcaaag gcgaaattgg gggtcagata gttttgcata
     4621 attgctcctg attgcggttt tgctcgggat tatgcaccgt ttttcaaagc agataaaggg
     4681 aaattgccga caatatcggg cagcggtaaa aataaaccga aatattttca atcggatagg
     4741 tgaaaaatca aaaaaatttc gataaaatgc ttgaggcgcg gcagcgtttt gatttagaat
     4801 ctccccaata tttttatctt cagacggcat ttcatcatgc aaggcaaaaa atttacaacc
     4861 gcttattgct attggtacca cacagacgtg cggtactttt ttgcgcttgc ttgaaatcca
     4921 tcccatacgc aaaacaggcc gctcgaaaac ggggcggctt ttttgttacc ttcacacagg
     4981 tcgcccgatt cggcaacaca cgacagaatc aggaaaatga ccatgaatgc aatgccccaa
     5041 accgccgcac aaaacgaaac cctgcacact tgtagcatta ttgtcgatga tgcacaggat
     5101 ttttccccca tgcttctcga tgcagggcgg gacgacacac tcatcagcga atcgatgatt
     5161 ccgcaaatcc gcactgttgc cgccctgata gccgccgaac ggcacgactt cagccgcagc
     5221 agccccgccg agtttaccga tgccgccgat ttttttgccg cacggattct cgtattgggc
     5281 gtgcgacgct tccatctgga cgtatcgctg cttcagattt tgaaaaccgc caacagccgc
     5341 gcccgccgct ttgccgaaaa acaccgcctg tttttcacac ccgcacaagc cgaattgagc
     5401 ctgaacaaac acaaaaaccg ccgcctgctg accgtagaaa ccgaacacga agtcgaaaac
     5461 aaaggcaacc cagtcgccaa cagcctcgca tttgtccgca aactgcacac cctgcccctg
     5521 taaacatcac gatttgtcgg cagaatgctt gccaaaattc ccgacggcga tacaataaaa
     5581 ccgattttcc acccaaacag gacacacatg aaaaaaatca tcgcctccgc gcttatcgca
     5641 acattcgcac tcgccgcctg ccaagacgac acgcaggcgc ggctcgaaca gcagcagaaa
     5701 cagattgaag ccctgcaaca gcagctcgca cagcaggcag acgatacggt ttaccaactg
     5761 actcccgaag cagtcaaaga caccattcct gccgaagcac aggcaaacgg caacaacggg
     5821 caacccgtta ccggtaaaga cgggcagcag tatatttacg accaatcgac aggaagctgg
     5881 ctgctgcaaa gcctggtcgg cgcggcggca ggcgcgttta tcggcaacgc gctggcaaac
     5941 aaattcacac gggcaggcaa ccaagacagt cccgtcgccc ggcgcgcgcg tgccgcctac
     6001 catcagtccg cacatcccaa tgcgcgcacc agcagggatt tgaacacgcg cagcctccgt
     6061 gcaaaacaac aggcggcgca ggcgcagcgt taccgcccga caacgcgccc gcccgccaat
     6121 taccgccgcc ccgccatgcg cggtttcggc agaaggcggt aaatccggcg tgccaatgcc
     6181 gtctgaaggg ctttcagacg gcatttttgt atttgttagg gacattgtta tgttgccgtt
     6241 tgatttttag acggcatttt gtttccaaga gtttgatgtc gggatggcaa ttctgattta
     6301 cgtgcgttgg cagggcggca ttgtcatgcc gccgttgggt tttcagacgg catcatatcg
     6361 aaccgtcagg tcgtggggtg agcattcttg cccaccgttg caagggcggg attttttggt
     6421 gaacaggaat gcccaccctg catctgaaac ccttctgcgt cattcccacg caggaaggaa
     6481 tttagatatt tgaatttaca gaaattttca atcgttgcca aagcggaaaa gg
//
LOCUS       DAWKOD010000109         6528 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000109 DAWKOD010000000
VERSION     DAWKOD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.109"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(216..899)
                     /locus_tag="WJK73_001549"
     CDS             complement(216..899)
                     /locus_tag="WJK73_001549"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ7558014.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1195..3498
                     /gene="parC"
                     /locus_tag="WJK73_001550"
     CDS             1195..3498
                     /gene="parC"
                     /locus_tag="WJK73_001550"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ7558015.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSG
                     SGGYGFIAKLADMVGRVKAGKVVMTVDSGETVLPPVAVYASSLINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            3518..5110
                     /locus_tag="WJK73_001551"
     CDS             3518..5110
                     /locus_tag="WJK73_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ7558016.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVLPLLIMHIFGLEVSGH
                     ENLHASWTTWAFYLWLAIACWLIFFSTLNPQWQWQALRIPSFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILDAK
                     TVTNTFVLVTGSYFVAMIASLSVRYIDRAGKLAHENHAAYRRIRGLNQIVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTALFDPVAVLWDKTSSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENAEEEETDPFKVKLCEIIDGNVRRIDKMLEDISSLNKRNKTEREAIDLMQFW
                     IGFKQEFLLNNPDAVGCIRLDMQGNHLTAYFDSAHLRQIMWNLCNNAWRHSSKRNGSI
                     SVTIRPAQKNTISILFADDGGGVPPEVQEHLFEPFYTTAKNGTGLGLYVARELAHANF
                     GDLTYLPEAKCFELALPEKSND"
     gene            5103..5570
                     /locus_tag="WJK73_001552"
     CDS             5103..5570
                     /locus_tag="WJK73_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ7558017.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVTEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            5674..6426
                     /locus_tag="WJK73_001553"
     CDS             5674..6426
                     /locus_tag="WJK73_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7558018.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1610 a         1987 c         1620 g         1311 t
ORIGIN      
        1 tcagaggttc tagattcccg cctgcgcggg aatgacggcg gagcggtttc tgcttttccg
       61 ataaatgacc gcaacctaaa acccatcctt cccacgaaaa cagaaaaaca aaaacctaaa
      121 atcccgtcgt tctcgcgaaa gcagttgcta cgtttaccgt caaaaatgcc gtctgaacgc
      181 tcaagccttc agacggcatt ttttatttca cacccttaca ggtagaattt ttcgatgact
      241 ttcaaattgt cgtccaattt gtacaccaac ggctgaccgg tcgggatttc caagcccatg
      301 atgtcttcgt cggaaatgcc ctcgatgtgt tttgccagcg cgcgcaggga gttgccgtgc
      361 gccgccacca agacgcgttt gccgctcaaa atcgcggggg cgatttggtc ttcccaaaac
      421 ggcaatacgc gctccagcgt tacttgcagg ttttcgccgt cggggataac atcggcaggc
      481 agatgggcat agcggcggtc tttgtgtgcg gaaaacgcgt cgtctttgtc caaaagcggc
      541 ggcagggtgt cgtagctgcg ccgccagatg cggacttgct cgtcgccgta ttgttcggcg
      601 gtttgttttt tgtccaggcc ttgcagtcgg ccgtagtggc gttcgttcag ccgccatgtt
      661 ttgatttgcg gtacgaacag ttggtcggat tcttccaaaa cgatgttgca ggtcttaatc
      721 gcgcgggtca ggacggatgt gaaggcgatg tcgaactcat agccgttttc tttcagtttt
      781 ttaccggcgg cagcagcctc ggcaagcccc tgctcgctca gcttcacgtc gcgccagcct
      841 gtaaacaggt ttttcgcgtt ccattcgctt tgtccgtggc ggataaatac caattccata
      901 tcgtctccaa tgtgtgaaag tgggaaagcc ttatttataa catattttca cattcccgta
      961 tttgattcag attcagacac gcgcccacta tggtttgccg ttttgattta caataatgcc
     1021 ctttgcttta cattccgcat acacaatgtt tcaacacaca ggacgacaca taaagcaccg
     1081 ccctatgtgt tgccctgatt tggaaggggt tgccccctcc caaataaagt ctgattctac
     1141 cgccccgaag gacagatgtc caagtggcgg gggttcaacc gaaaaggaaa tacgataaat
     1201 atgcaaccgc acgctccaca taccgattcc aatacgctga tgctcggccg atacgccgaa
     1261 cgcgcctatc tcgaatacgc catgagcgtg gtcaaaggcc gcgcgctgcc tgaagtttca
     1321 gacggccaaa agcccgttca gcggcgcatt ttgtttgcca tgcgcgatat gggtttgacg
     1381 gcgggggcga agccggtgaa atcggcgcgc gtggtcggcg agattttggg taaataccac
     1441 ccgcacggcg acagttccgc ctatgaggcg atggtgcgga tggcgcagga ttttaccttg
     1501 cgctatccct taatcgacgg catcggcaac ttcggttcgc gcgacggcga cggtgcggcg
     1561 gcgatgcgtt acaccgaagc tcggctgacg ccgattgcgg aattgctgtt gtccgaaatc
     1621 aatcagggaa cggtggattt cgtgccgaac tacgacggcg cgtttgacga gccgctgcac
     1681 ctgcccgccc gcctgcctat ggtgttgctc aacggcgcgt caggcatcgc ggtgggcatg
     1741 gcgaccgaga ttccgtcgca caatttgaac gaagtgacgc aggcagcgat tgcgctgttg
     1801 aagaagccga cgctggaaac cgccgacctg atgcaatata ttcctgcgcc cgattttgcc
     1861 ggcggcggtc aaatcatcac gccggcggac gaattgcgcc ggatttacga aaccggcaaa
     1921 ggcagcgtgc gcgtgcgtgc gcgttatgaa atcgagaagc tggcgcgcgg acagtggcgc
     1981 gtcatcgtaa ccgagctgcc gccgaacgcc aattccgcca aaatccttgc cgaaatcgaa
     2041 gaacaaacca acccgaaacc gaaagcgggt aagaaacagc tcaaccaaga ccagctcaac
     2101 accaaaaagc tgatgctgga tttaatcgac cgcgtgcgcg acgagtccga cggcgaacat
     2161 cccgtgcgac tggtgttcga accgaaatcc agccgcatcg ataccgatac cttcatcaac
     2221 acgctgatgg cgcaaacttc gctggaaggc aatgtgtcca tgaacttggt gatgatgggg
     2281 ctggacaacc gccccgcgca gaaaaacctg aaaacgattt tgcaggaatg gctggatttc
     2341 cgcaccgtaa ccgtaacacg ccgtctgaaa ttccgtttga accaagtgga aaaacggctg
     2401 cacatcctcg aaggccgtct gaaagtcttt ctgcacatcg acgaagtgat taaagtcatc
     2461 cgcgaatcag atgacccgaa aaccgatttg atggcggcgt tcgggctgac cgaaatccaa
     2521 gccgaagaca ttttggaaat ccgcctgcgt cagttggcgc gtttggaagg tttcaaactc
     2581 gaaaaagaat tgaacgaatt gcgcgaagaa caaggccgtc tgaacatcct tttgggcgac
     2641 gaaaacgaaa aacgcaagct gattatcaaa gagatgcagg cggacatgaa gcagtttggc
     2701 gacgcgcgcc gcacgctggt ggaagaggcc ggacgcgccg tgctgacaca aaccaccgcc
     2761 gacgaaccca tcacgctgat tctgtcggaa aaaggctgga tacgcagccg tgccggacac
     2821 aacctcgatt tgagccaaac cgcgttcaaa gaaggcgacc gcctcaaaca aactctcgaa
     2881 ggccgcactg ttttacccgt cgtcatcttg gattcattgg gcagaaccta cacgctcgat
     2941 gccgccgaaa tccccggcgg acgcggcgac ggcgtaccgg tttcatcctt aatcgagctg
     3001 caaaacggcg cgaaacccgt tgcgatgctg acaggattgc cggaacaaca ttatttatta
     3061 tcgggcagcg gcggctacgg cttcatcgcc aagttggctg atatggtcgg acgcgtgaaa
     3121 gcgggcaaag tggtcatgac cgtggacagt ggcgaaaccg tcctgccgcc ggttgccgtc
     3181 tatgcttctt cgctgatcaa cccagactgc aaaatcattg ccgccaccaa tcaaaaccgc
     3241 gccctcgcct tccccatcgg cgaattgaaa attatggcga aaggcaaagg actgcaaatc
     3301 atcggattaa acgccggcga atcgatgacg cataccgccg tttcttccga gccggaaatc
     3361 ctgattgaaa gcgaaggcag gcgcggcgcg gcgcacaaag accgcatccc tgtctccctg
     3421 cttgaggcaa aacgcggcaa aaaaggcaaa ctattaccca tatcgggcag cctgaaacag
     3481 ctttcttccc cgaaataaac ccgactctgc acatactatg gtaatttcca atacccgcga
     3541 acttgagaaa ctcaaagacc ggattcccaa tctgatcaac atcgcccgca tcgccatcgt
     3601 cctcccccta ctgattatgc atatcttcgg gttggaggtc agcgggcatg aaaacctgca
     3661 cgcctcatgg acgacatggg cgttttactt gtggcttgcc attgcctgct ggctgatttt
     3721 cttttccacc ctcaacccgc aatggcaatg gcaggctttg aggataccga gtttcagtgc
     3781 cgtggccgac atcacgatga tcggcgtgct gacctacctg ttcggcggca tcgattcggg
     3841 gttcggcatc ctgatcctac ccttcgtggg cagctcctgc ctactcagtt acgggcgtta
     3901 tcccctgctc tatgccagct atgcatccat cctgctgata ttcaacgccc ttgcagacag
     3961 caatatcaat atgtatccgc tgattctgga tgcaaaaacc gtcaccaaca ccttcgtcct
     4021 cgtgaccggc tcctatttcg tcgccatgat tgcctcgctg tcggtcagat acatcgaccg
     4081 tgccggcaaa cttgcccatg aaaaccacgc cgcctaccgc cgcatcaggg gcttgaacca
     4141 aatcgtgctc aaccgcgttc aggaagcggt cgtcgtcatc aacgtcgagc atcaaaccat
     4201 actgttcaat aaaaaggcaa aagacctgct acccatgctt gaaatcggac agcataccgc
     4261 cctgttcgac cctgtcgccg tcttatggga taaaacatct tcacgtactt tcgaacgcaa
     4321 tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg ccgatgaaca aagagcaaaa
     4381 caagctgctc atcctctaca tccgcccgca aagcgaaatt caggcagaag ccctgtccgt
     4441 caaacttgcc gcactcggac aactgaccgc caacctcgcc cacgaaatcc gcaaccccat
     4501 gtccgccatt cgccatgcaa acgacctgtt gcgcgaaaat gcagaggaag aagaaaccga
     4561 cccattcaag gtcaaacttt gcgaaatcat cgacggcaac gtccgccgta tcgacaaaat
     4621 gctcgaagac atctcctcgc tcaacaaacg caacaaaacc gaacgcgaag ccatcgacct
     4681 gatgcagttt tggataggct tcaaacagga attcctgctc aataatcccg atgccgtcgg
     4741 ctgcatccgc ttggatatgc agggcaatca cctgactgcc tatttcgatt ccgcccactt
     4801 gaggcaaatt atgtggaacc tgtgcaacaa cgcatggcgg cacagcagca aacgtaacgg
     4861 ctcgatttcc gtcactatcc gccccgcgca aaaaaacacc atcagcatcc tgtttgccga
     4921 cgacggcggc ggcgttccgc ccgaagtgca ggaacacttg ttcgaaccct tttacaccac
     4981 agcaaaaaac ggaaccggct tgggactgta tgtcgcccgc gaactggcac acgccaactt
     5041 cggcgatttg acctacctgc ccgaagccaa atgtttcgaa ctcgcattac cggaaaaaag
     5101 caatgactga actgcaacat cccgttctcg tcgtcgatga cgaaaccgat attctcgacc
     5161 tgatggaaat gaccctgatg aaaatggggc tgcgcgtcca taccgcgtca ggcgttaccg
     5221 aagcaaaaaa caagctcgac agccaacgct attcgctcgt cctgaccgat atgcgtatgc
     5281 cggacggctc ggggctggaa gtcgtccaac acatcaacag ccgcctgctc gatacgccgg
     5341 ttgccgtcat caccgccttc ggcaacgccg atcaggcaca ggaagcgttg cgttgcggcg
     5401 cgttcgaccc cgataccatg cagatacagg actatctcga ccaaatcgaa cgcgacatta
     5461 tcgaacaaac cctcaaacaa accgaaggca accgcacgca ggccgccaaa cgcttgggca
     5521 tcagcttccg ttccatgcgc taccgtatgg aacgcctcaa catcggctga cgacaaaacg
     5581 gcatccgcac catctccgcc cacccgaaaa aatgccgtct gaaacggcac gggaaagcgg
     5641 gttcgcccca cgcccgaacg gacacaaaac accatgaccg acatccttat tgacaacacc
     5701 gccaccgaaa ccgtccgcac cctgatacgg gcattccccc ttgtgcccgt ttcccaaccg
     5761 cccgaacaag gcagttacct ccttgccgaa cacgataccg tcagcctcag gcttgtcggg
     5821 gaaaaaagca gcgtcatcgt cgattttgcc tccggcgcgg cacaataccg gcgcacaaaa
     5881 ggcgggggcg aactcatcgc caaagccgtc aaccacaccg cgcaccccac cgtttgggac
     5941 gcaaccgcag gattggggcg cgacagcttc gtcctcgcct cgctcgggct ggccgttacc
     6001 gccttcgagc aacatcccgc cgtcgcctgc ctgctttcag acggcatccg ccgcgccctc
     6061 ctcaatcccg aaacgcaaga caccgccgcg cgcatcaccc tgcatttcgg caacgccgca
     6121 gtacaaatgc ccgcacttgt tcaaacacaa ggcaaacccg acatcgtcta tctcgacccc
     6181 atgtatcccg aacgccgcaa aagtgccgcc gtcaaaaaag aaatgaccta cttccaccgg
     6241 ctcgtcggtg aagcgcaaga tgaggccgcc ctcctccata ccgcacgcca aacggcaaaa
     6301 aaacgcgtcg tcgtcaaacg cccccgcctc ggcgaacacc ttgccggaca agcccctgcc
     6361 taccaataca ccggcaaaag cacccgcttc gacgtttacc tgccctacgg gacggacaag
     6421 ggataacgcc cataaaacaa gacaccgaaa aatttgccgt tcttatgcaa acgagaaacc
     6481 ggtttttgcg tttcgactgt tttggataaa tcatcacacc ttaaagtt
//
LOCUS       DAWKOD010000110         6487 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000110 DAWKOD010000000
VERSION     DAWKOD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.110"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..1465)
                     /locus_tag="WJK73_001554"
     CDS             complement(110..1465)
                     /locus_tag="WJK73_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ7558019.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1662..2483
                     /gene="nadE"
                     /locus_tag="WJK73_001555"
     CDS             1662..2483
                     /gene="nadE"
                     /locus_tag="WJK73_001555"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ7558020.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2553..3026)
                     /locus_tag="WJK73_001556"
     CDS             complement(2553..3026)
                     /locus_tag="WJK73_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7558021.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3329..3661
                     /gene="trxA"
                     /locus_tag="WJK73_001557"
     CDS             3329..3661
                     /gene="trxA"
                     /locus_tag="WJK73_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ7558022.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWASWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3714..4661)
                     /locus_tag="WJK73_001558"
     CDS             complement(3714..4661)
                     /locus_tag="WJK73_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7558023.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            4989..6377
                     /locus_tag="WJK73_001559"
     CDS             4989..6377
                     /locus_tag="WJK73_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7558024.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1542 a         1749 c         1712 g         1484 t
ORIGIN      
        1 cggttttttg aatttcagtc atttctgata aaccccggcg gctttccgct tccggactcc
       61 cgcttttgcg ggaatgacgg cggaggggga gcggtttttc cgattgggtt taaatgcaat
      121 cgaacaaatc ctgctgccct tgttctttgc ttacgcgcac gtcggtttcg ccgtcggcga
      181 aggtaatgtg cagcttctgc ccctgcttca aaacatcggc gttgcggatg acttgtccgc
      241 gtgtgttttt gacgacggaa aagccgcgct ccagaatgtg ctgcggcgaa acggcttcga
      301 gcaatgcggc ttgggcggtc aggctttggc ggcggtgggt aagcagttgg ctgaaggcgg
      361 cgggcaatgc cgtctgaagg cggacgatgt tgtttttgca aacggaaaca tcaggacggt
      421 aatgttgcag ggcttgggtt tggcgttcaa aacgggcggt gtgggcacgg aggttttgct
      481 tcatcgagta agacagcgtt tgcgccagct tgccgattga agcgcgctgc tcatcaagtt
      541 tttggcgcgg atgacggatt tgccgcgcaa gccagtcgag tttttggctg gcatcgaaat
      601 agcgttgttc caaaacggtt ttcagacggc cttgcgcctg agcgagacga tgtagcgatt
      661 cttggcggtt ggggctgacc agctccgccg caccggtcgg cgtgggcgcg cgcacgtcgg
      721 cgacaaaatc ggcaagcgta aaatcggttt cgtgtcccac gccactgacg acgggaaccg
      781 tgcaggcttc gattgcgcgc acgaccggtt cttcgttaaa cgcccacaag tcttcaatgc
      841 tgccgccgcc gcgacagaca atcaacacgt cgcattcggc gcgttgcgag gcggttttaa
      901 tcgcttgggc aatttgtaac tcgctgcctg cgccttgaac aggtgtcgga taaacgataa
      961 cgggaatttc gggcgcgcgg cgtttcaagg tggtaacgac atcgcgcaat gccgctgctg
     1021 ccaggctggt gacgatgccg atacattgcg gacgggtagg caacggcttt ttgcgttccg
     1081 ccgcaaacgc gccttccgcc tgcaactgtg ccttcaaccg ctcgtaggct tcgtaaagct
     1141 gccccaaacc tttgagccgc acctcattca cggtaatttg aaattcgccc cgcgcctcgt
     1201 aaatactgat ttttcctgat acttcgatat ggtcgccttc tttcaaaggc ttcgccaaac
     1261 gcgccgccgc acctttgaac atcgcgcaac gcacctgcgc gcggctgtct ttgagcgaga
     1321 aataataatg tccgctggcg gcacgggtca ggttggacac ttcgcccgca atccacaggc
     1381 cggcaaggtg gttttccaaa agacttttgg caaatgcgtt caactcagaa acggacaaca
     1441 cgtcagaatg aaaaaaatca gacatagaat caatcaaata ataaaaaata tgaatatgtt
     1501 ttgaagccta aggcggtatt agtccgccta aattgtcaac aatattataa cacgcgccat
     1561 cttgccgccc gccttttccc gtatgacttt tttaagcggg gaatgggaaa aatattcatc
     1621 aacccgccct caatctattc aaattacacc gccgacaagc tatgatgcgg atattttcga
     1681 caggaggaaa aatggatacg caggcagtta tcacacatat tgtccgatgg ttggacgaat
     1741 acgccgcccg ggcaaatgca aaagggtttg tcgtgggcgt ttccggcggc atcgattccg
     1801 ccgtcgtctc cgcactcgcc gcccgcaccg gccgcaccac gctgcttctg gatatgccga
     1861 tacgccaaca ccccgaccag cttgagcggg caaggctgca catccgcaat ctgcaacggc
     1921 aatatgccaa tgtaagcgca caaacggtcg atctgaccga caccttccag acctttgaac
     1981 aaaccgtcgg cgttcatcag acggcatttg acaatcagcc gctttccctc gccaacgcca
     2041 gaagccgcct gcgtatgctg accctgtact actacgggca gatacacgga ctactggtta
     2101 cggggacagg taacaaaatt gaagatttcg gtgtaggatt ttttaccaaa tacggcgacg
     2161 gcggcgtgga catcagcccg attgccgacc tgaccaaaac ccaggtttac cggcttgccg
     2221 aagcattggg cgtggacgag gcgattcaaa aagccccgcc gaccgacggc ctgtgggata
     2281 cggaacgcac cgacgaagaa cagatgggcg caagctatcc cgaactggag tgggcaatgg
     2341 gcgtgtacgg cacgcgcaaa cccgaagatt ttgaagggcg gcagcgcgaa gttctggaaa
     2401 tctatacgcg gctgcaccgc gcgatgcagc acaaaatcaa cccgattccc gtgtgccgca
     2461 ttccgcccga attgttgggc tgaaacgtgg aaatgccgtc tgaagcggaa aaccgtgttt
     2521 cagacggcat tgacatatcc ggctcctatc ccttaagaat cgagtacgcg ggcaaacaaa
     2581 atatcgtttt ccaaatgaat gtggtcgttc aaatcctcca ccatttcttt ggccaaagcg
     2641 taaagccgcg tccagcttcc gcaggctcct tcgggcgctt ggaaattatc ggtcagctct
     2701 ttgagccttg cgatggcgcg gtcgtgttct tcatgttcgt gcatcatcac gctgatgggc
     2761 atcgccgcac cccgaccgac accctgatta atcatcggaa acagcatcct ttcctctttc
     2821 atcatatgca tcagcagttc gttctgcata taggcaagca gctcggcaat ttccgccgga
     2881 aaggtatcgg catgaacttg ggccactttt tgcgccagcg gcaccaattc ttcaaattgt
     2941 gcacggtgta cattgtggta gcgttgcagg atatgatcga cggttgcacc aaagggagca
     3001 gtttcccaaa cggaaaaatc agtcatggca gggttccttt tatttatagg gtttcgggtt
     3061 tggttttgaa cattcatact ttaagaatct atttaaacgg agcatacacc gcccgtgcgt
     3121 ttctgtacag cttcaaaaac attccttaca ttttgataat aaaagtaatt ttcaaaaata
     3181 aaatactgtc cgaaccgttt ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa
     3241 ctattatccc gcccgcccac ttgaaatttt tatgcccaag ccctatcctg cacgctatcg
     3301 tgccaatccc aaccgaaaag gaaaaataat gagcagcgaa ttgatcgtac acaccggcga
     3361 tgccgccttc gagcaagatg ttttgaaatc cgacctcccc gtcctgctgg acttttgggc
     3421 ttcgtggtgc ggcccgtgca aaatgattgc cccgattttg gacgacattg ccgccgaatt
     3481 tgaaggccgt ctgaaagtag tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg
     3541 tttcggcgtg cgcggcattc cgaccctgat ggtgttcaaa aacggcgacg tcgtcgccac
     3601 caaagtcggc gcattggcaa aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg
     3661 ataaagcgca atcgaaaaag ccgccggaag attccggcgg ctttttcgca cccttaagat
     3721 ttgtggcgga tttcccagca ccgatggatt tttttgttgc ggaaatcttc gggaacggat
     3781 tgtttggaaa tgtctttgac ggcgtattgt tccgatacca agtcgtctaa gacgaagctg
     3841 cgcaggttgt tggaaaagta caaaatgccg tctgaagcga gcagcttcac cgcgccgtca
     3901 atcagctttt tgtgatcgcg ctgaatgtcg aggatgtcgg acattttctt gctgttggaa
     3961 aaactgggcg gatccatcac aatgaggtcg aactgcctgc cttccccata tgccgtctga
     4021 agatattgga acacgtcggc gcggacgatt ttgtgccgtt ccgtatcgat gccgttcaat
     4081 tcaaaattgc gtctcgccca atcgagataa gtgttggaca aatcgacagt ttcgctggat
     4141 gccgcgccgc cggtggcggc atagacagtg aagctgccgg tgtaggaaaa caggtttaaa
     4201 aaacgtttgc ccgccgccgt ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa
     4261 agccccgtat ccaaatactt atcaaggttg acccaaaact tgcggccgtt ttcggtgatg
     4321 acgaaatcgt cgcccgcctt gccggttttt tcgtattgct gcaaaccttt ttggcgttcg
     4381 cggcgtttga ggcggatttg ttcgggcgca aaaccggtaa cgaaggcgac ggcttccaag
     4441 acttcggcaa gccacgcttc gtattcttcg gaccgcatca gccagccggt atcgtattcc
     4501 tgaaggtgga tttggtcgcc gtaaacatcg acagcaaagg ggaattgggg gatgtcgcgg
     4561 tcgtaaatgc gccaggcttc gatgccgttg cgtttcgccc atttcataag gtgtttgatg
     4621 tttttgccca agcggttggc aaacggtgtg atgtcggtca ttggtttcag gcggaataaa
     4681 gtggaaaacg gcaattttac tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc
     4741 ctataccatc cgtcgcttat cttgtcatac gaagccatcg ccttccaacc taaaccgccc
     4801 ttacgggcgc gtttcttctg ttgctttgat tttgcaaagc gtatctgtgc aggttgccgt
     4861 cgatgtaaac cacaagcaag ccgcttgcga caaccctgta acttcacatt ccccgtatcg
     4921 ttacccttcc ctgcttcagg ccgtctgaac ctttcggacg cgagcgttgt tgtcttccaa
     4981 ggatagccat gtctattaaa tttgccgatt tgaaccttga taaaaacatt ttgtccgccg
     5041 tcagcagcga gggttacgaa agcccgacgc cgattcaggc gcaagccatt ccgtttgctt
     5101 tggaaggccg cgacatcatg gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc
     5161 tgttaccgac tttgcagcga ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgcg
     5221 cgttggtgtt gacgccgacc cgcgaattgg cggctcaagt cgagaaaaac gcgctggcgt
     5281 atgccaaaaa tatgcgttgg ttccgtaccg tcagcatcgt cggtggtgca tcgttcggct
     5341 accaaacccg cgccctgagc aaaccggtcg atctgattgt cgccacgccg ggccgtctga
     5401 tggacttgat gcaaagcggc aaagtcgatt tcgcccgctt ggaagtgctg attttggacg
     5461 aagccgaccg tatgttggat atgggcttta tcgacgacat cgaaaccatc gtcgaagcca
     5521 cgccggaaga ccgtcagact ttgttgttct ccgccacttg ggacggcgcg gtcggcaaac
     5581 tggcgcgcaa actgaccaaa gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag
     5641 gcaaaatcga agaacaactg ctgtactgcg acgatatgcg ccacaaaaac cgcctgctcg
     5701 atcatatctt gcgcgatgcc gacatcgatc aatgcgtgat tttcacgtcc accaaagcca
     5761 tgaccgaagt catcgcggat gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg
     5821 gcgatatgcc gcaaggctgg cgcaaccgca cgctgatgga tttgcgcaaa ggccgctgca
     5881 aaattttggt tgccaccgac gttgccgcac gcggtatcga cgtaccgacc attacccacg
     5941 ttatcaacta cgacctgccg aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg
     6001 gccgcgcagg ccgcacgggt attgcgatta cgtttgccga agtgaacgaa tacgtcaaag
     6061 tccacaaaat cgaaaaatac attaaccgaa aactgcccga actgaccatc gaaggcatgg
     6121 aaccgacccg caaacgcaaa tccgcaggcg gcaagccgaa aggcaaaggc ggctggggcg
     6181 atcgtaaatc cggcggttgg cgcggcgatc ataaaccgag caaagaaggc ttcggcggca
     6241 aaacgcgcgg cgaaggtttc aagaaagaag gctttaagag agacggtttc aaaaaaaccg
     6301 gcgaaggctt caaaggcaaa cgcaaagccg gcgattcttt tgcaggcaaa ggcgaacgcc
     6361 gttacaaaga ccgctaagcc ccaacctgcc gcataaacca atgccgtctg aaaccgattt
     6421 cgagtttcag acggcatttt tgcaatgttt cagcaccgcc cggctttgat acccaaagga
     6481 ttaatct
//
LOCUS       DAWKOD010000111         6381 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000111 DAWKOD010000000
VERSION     DAWKOD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.111"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..623)
                     /locus_tag="WJK73_001560"
     CDS             complement(63..623)
                     /locus_tag="WJK73_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ7558025.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJK73_001561"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJK73_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ7558026.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3326
                     /locus_tag="WJK73_001562"
                     /pseudo
     CDS             2243..3326
                     /locus_tag="WJK73_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3349..3567)
                     /locus_tag="WJK73_001563"
     CDS             complement(3349..3567)
                     /locus_tag="WJK73_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558027.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3582..4220)
                     /locus_tag="WJK73_001564"
     CDS             complement(3582..4220)
                     /locus_tag="WJK73_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ7558028.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4401..6236)
                     /gene="edd"
                     /locus_tag="WJK73_001565"
     CDS             complement(4401..6236)
                     /gene="edd"
                     /locus_tag="WJK73_001565"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ7558029.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIHSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1419 a         1534 c         1795 g         1633 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa atttaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ctaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcaa ccggtgcgtc agccggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 ttttcgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gggtttccaa gtctttgcgc tccgacggaa acgtgccgcc gaattgttcg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggcatataa ctcatataag gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa cccgaagtcg
     2401 ccgacggccg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgaggctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgccgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgca ggcagaatac gtccgcacgc
     2641 aggcagaata cgtccgcacg ccttatgccg acaacagcct cgtgccgctg cccgacaacg
     2701 tcaacgaaga agtcgccctg ttgttgagcg acgctttgcc gaccgcacac gaaatcggcg
     2761 tgcaatacgg cgatgtcaaa cccggcgaca ccgtattcat cgcaggtgcc gctcctgtcg
     2821 gtatgtccgc cttgccgacc gcccaactgt acagccccgc cgccatcatt gtttgcgata
     2881 tggacgaaaa ccgtttgaaa ctggcgaaag agttgggcgc aacccatacc attaaccctg
     2941 cttccggcga tgtaagcaag caagttgccg ctatcgtcgg cgaagacggc gtggactgcg
     3001 cgatcgaagc cgtcggtatc cctgcaactt ggaatatgtg ccaagacatt gtgaaacccg
     3061 gcggccatat cgccgttgtc ggcgtacacg gtcaatccgt tgatttcaaa ctggaaaaac
     3121 tgtggattaa aaagcttgcc atcactaccg gcctggtaaa cgccaatacc accgaaatgc
     3181 tgatgaaggc aatttccagc agctccgtcg attacaccaa aatgttgacc caccgtttca
     3241 aattcagcga attggaaaaa gcctacgacg tgttcaaaca cgccgccgaa aatcaagcga
     3301 tgaaagtggt tttggaagcg gattaatcgg cgttgaatca gttgtttttt aagcggatgc
     3361 cgtctgaatg cgtttcagac ggcatttttt cagccgcgta gtatgggctg tccggctgct
     3421 gtcgaatgaa ctgaacgtta tttttattat gcggaaggcg gatgggcatt ttaggggcag
     3481 tatgtcggag tggtgctgcc gtgtatgccg actgcgccgc caagcggcaa aatgccgtct
     3541 gagactggaa agggcttcag acggcatttt tacgatgcgg ttcaggcttt gggtttcaat
     3601 gccgccgctt ctttggcgag acgggtgatg gtgtcccagt ctttgttttt cacggcttct
     3661 ttcggcgtca gccatgagcc gccgacgcac aggacgttgg gcagtgccaa gtaatcgggc
     3721 gcggtggcga ggctgatgcc gcctgtcggg cagaagcgaa catcggcgta agggccgtac
     3781 agggctttga gcatggcttt gccgccaacg acttcggcag ggaagagttt gagggtgtcg
     3841 atgccgtgtt ccaaagccag ttggatttcg cccggggtgg caacaccggg aatcaggggg
     3901 atgccgctgt tgtggccggc tttggcgagg gattcgtgca aacccgggct gatggcgaaa
     3961 accgcgcctg cgtcttcgac ggctttgagc tgttcagggt tggtaaccgt acccgcgccg
     4021 acgatggcgt tgggcacttc tttggcaatc aggcggatgg catcgaggcc gacaggggtg
     4081 cgcagggtga tttcgagggt ggggatgccg ccttcgacaa gggcgtggga caaatcgacg
     4141 gcggtgctta agtcgtcaat cgccattacc ggcacaactg cgccggcggt caaaatttcg
     4201 cggggggtca gtttggacat ttcggttctc cgggtggaat ggggtatttt attaagatgg
     4261 gacaggtcgt aggtcggatt caagaaaccg acatcttgga cggctgtgtc ggattcgaga
     4321 atccaaccta cgtctatctt aaaacacttg aataattttt cagacggcct tcggtctgaa
     4381 aaggccgtct gaaacgcgca tcaggcaaat tcgccgccga aactcatggc accggtttcc
     4441 gcgctgctgg tcatgctgcg gaagttggcg aagagttcgc ggccgcagcc ttgttggttc
     4501 gcgcccaagt cgatgctttc gacttcgcgg gcattccatt cggtttcgtt aatcaggacg
     4561 ttgagttcgc cgctaacgga gtcgaagcgg atcaggtcgc cggtacggat tttggcgatg
     4621 ttgccgccca tcagggcttc gggtgtcatg tggatggagg ccggaacttt gccagatgcg
     4681 ccggacatac gaccgtcggt cagcagtgcc actttgaagc cgcggtcttg caggatgccc
     4741 aaaggcgggg tcagtttgtg caattcgggc ataccgttgg cacgcgggcc ttggtagcgg
     4801 acgacgcaga cgaaatcgcg ttccaactcg ccgcgttcaa atgccgccaa cacttcgcgt
     4861 tggtcgttga acacgatggc aggcgcttca ataatgcggc agccttcgcg cacggcggac
     4921 actttaatca cgccgcgtcc gatgttgcct ttcatcaggc gcagaccgcc gtcgggggag
     4981 aacgggttgc cggctttgcg caggatgtcg tcgttgccgc tggtttcggg ggcttcgcgc
     5041 cattcgagtt tgccgtcgat aaggaaaggc tctttggtgt agtggcgcat accgtgtccg
     5101 acaacggtat cgacatcgtc gtgcaacagg cctgcattca gcaattcgcg gataacgaaa
     5161 ggtaggccgc cggcagcggt aaagtggttt acgtcggctt tgccgttggg gtacacacgg
     5221 atcagcaatg ggatgatgga agaaatttcg tcaaagtcgt cccagttcaa aatcacgccg
     5281 gcggcgcgtg ccatggcaac gaggtgcatg gtgtggttgg ttgagccgcc ggttgccatc
     5341 aggccgatta gggcgttgat gaaagatttt tcggtcaaca tttcacccaa tggtttaatc
     5401 gtgccgtttt tgatgccgcg cgcgaggtgt ccggcggcat agcgggtcag ggcttcacgc
     5461 aggtcggtgt aagggtggac gaaggcggcg gcaggcaggt gtacgcccat catttccatc
     5521 atcatttggt tggagtttgc cgtgccgtag aaagtgcagg tgcccgggct gtggtaagaa
     5581 cccatttcgc ttttcaaaag ttcgtctcgt ccgactttgc cttcggcgaa aagctggcgg
     5641 gtgcgggctt tttctttgtt gccgatgccg ctggacatcg ggcctgcggg gacgaagata
     5701 cccggaatat gaccgaacga aagcgcgcct atcatcaaac ccggcacgat tttgtcgcat
     5761 acgcccataa acaggctgcc gtcaaacatt tgatgcgaca gaccgatagc ggtactcatg
     5821 gcgatcacgt cgcgggaaaa cagcgacaat tccataccgg cgtagccttg cgtgatgccg
     5881 tcgcacatgg cgggtgtgcc gccggcgact tgggcggtcg cgccgttttt ctgcgcttcg
     5941 tctttaattt ggtcggggaa gtctttaaac ggctggtgtg cggaaaccat gtcgttgtag
     6001 gcggtgatga tgcctaagtt ggggacggtt tcctggagca tttcgatttt gatgcttttg
     6061 ggcatagagg catagccgtg cgccaagttg gagcagccga gctggttgcg ctctaagcgt
     6121 cccatttgtt tggcactgtg gattttcgcc agatattttt cacgcgtcgg acggctgcgt
     6181 tcaataatgc gcccggtgat ttcggcgagt ttggagtgga taggagtagt gttcacggtc
     6241 ggtctcctgt cggaacgggg ggtattgggg atttgtttgt gcgtttctga ttttttgtat
     6301 tgggtgtggg gcatttggcg tttcagacga cctttcaaac cttacaaggg gtcgtctgaa
     6361 aacctcacta tagtggatta a
//
LOCUS       DAWKOD010000112         6316 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000112 DAWKOD010000000
VERSION     DAWKOD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.112"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..998
                     /gene="hupA"
                     /locus_tag="WJK73_001566"
     CDS             <1..998
                     /gene="hupA"
                     /locus_tag="WJK73_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HEZ7558030.1"
                     /translation="VPVSIPTATPLPKGEVTLSSDNGNIENINTANTETHPPRRTRRS
                     LYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVPSYSS
                     PDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEIDLFAG
                     GFPIGKTPPPAFSWGSPTTETALSKGKITYQVWGIRVRNGQFVTSSYTPPKGSSWSGY
                     QNTPVLSFITANFNSNKLAGEIIGNSDYGPNVKIENATIDGLSFSGDATSNGKTGNLE
                     GKFFGKFNSTRSSDTGIGGKITFKDDRSLDTVFGGVIYEKKLDDKTSQDTNHLKK"
     gene            1029..3461
                     /gene="hupB"
                     /locus_tag="WJK73_001567"
     CDS             1029..3461
                     /gene="hupB"
                     /locus_tag="WJK73_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ7558031.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4049..5683)
                     /gene="groL"
                     /locus_tag="WJK73_001568"
     CDS             complement(4049..5683)
                     /gene="groL"
                     /locus_tag="WJK73_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ7558032.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5776..6066)
                     /locus_tag="WJK73_001569"
     CDS             complement(5776..6066)
                     /locus_tag="WJK73_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ7558033.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1536 a         1863 c         1538 g         1379 t
ORIGIN      
        1 acgtccccgt gtccatcccc accgccacgc cgctgcccaa aggcgaggta acgttatcaa
       61 gcgataacgg caatatcgaa aacatcaaca ccgccaacac tgaaacacat ccgccgcgcc
      121 gcaccagacg ctccctctat gcctccccgc aaaacacatc ctccggtatt tccattcagc
      181 agcgggaagt agaaaaaatc tatttcggtg ttaaatcacc agaaaaatca ttcatcttcc
      241 aaacacccgg cggcgcgcaa tacgcccttt catcctacgc cgaccccatc gtccccagct
      301 actcctcccc cgacttcaaa attcccgacc gccacgcagg gcagcggctt gccgacggca
      361 gccgcatctt tatctgctgc agcgactccg gggctacttc gtatgcggaa attaccaaac
      421 aagattatat gaaattcggg gcgtggatag gccccaacgg cgaaatcgac ctcttcgccg
      481 gcggcttccc catcggcaaa acgccgcctc ctgcgttctc ctggggcagc cccacgaccg
      541 aaaccgcatt gtcaaaaggc aaaatcactt atcaggtttg gggcatccgc gtcagaaacg
      601 ggcaatttgt tacttcctct tatacgccgc ccaaaggcag ctcttggagt ggttatcaaa
      661 acacccccgt cctttccttt atcaccgcca atttcaacag caacaaactg gcaggcgaaa
      721 tcatcggcaa cagcgattac gggccaaatg tgaaaattga aaacgcaacg attgacggac
      781 tatccttttc cggcgatgcc acctccaacg gcaaaaccgg caacttggaa ggcaagtttt
      841 ttggcaagtt caacagcacc cgcagcagcg acaccggcat aggcggcaaa atcactttta
      901 aagacgaccg ctccctcgat accgtattcg gcggcgtgat ttatgagaaa aaacttgatg
      961 acaaaaccag tcaagatacc aaccatctca aaaaataata acccatccgc ttttaaaaag
     1021 gttttcttat gcccatcccc tttaaacccg tattggctgc cgccgccatc gcccaagcgt
     1081 ttcccgcctt tgcggcagac cccgcgccgc agtccgccca aacgctgaac gaaatcaccg
     1141 ttaccggcac gcacaaaacc caaaaactcg gcgaagaaaa aatccgccgc aaaactttag
     1201 acaagctctt ggtcaacgac gaacacgacc tggtgcgcta cgaccccggc atttccgtcg
     1261 tcgaaggcgg cagggcgggt tctaacggct ttaccatacg cggcgtggac aaagaccgcg
     1321 tcgccatcaa cgttgacggg ctggcgcagg cggaaagccg ctcttccgaa gccttccaag
     1381 aattgttcgg cgcgtacggc aacttcaacg ccaaccgcaa cacttccgag ccggaaaact
     1441 tttccgaagt aaccatcacc aaaggcgcgg actcgctcaa atccggcagc ggcgcattgg
     1501 gcggcgcagt caattaccaa accaaatccg caagcgatta tgtttccgaa gacaagccct
     1561 accatttggg gataaagggc ggcagcgtcg gcaaaaacag ccaaaaattc agcagcatca
     1621 ccgccgccgg caggctcttt ggtttggatg ccttattggt ttatacccgc cgcttcggca
     1681 aagaaaccaa aaaccgctcg accgagggcg atatcgaaat taaaaacgac ggatatgtct
     1741 ataacccgac cgatacaggc ggacccagca agtacctgac ctatgtagcc acaggggttg
     1801 cgcgctccca acccgacccg caagaatggg taaacaaaag caccctgttc aagctgggct
     1861 acaacttcaa cgatcaaaac cgtatcggct ggatttttga agactcgcgc accgaccgtt
     1921 ttaccaacga gctgtctaat ttgtggacgg gtacgaccac gtctgccgca acgggcgact
     1981 accgccaccg ccaagacgtg agctaccgcc gccgctccgg tgtcgaatac aaaaacgaat
     2041 tggaacacgg cccgtgggac agcctcaagc tgcgctacga caagcagcgc atcgatatga
     2101 acacttggac ttgggacatc ccgaaaaatt acgatttgcg cggcatcaac ggcgaggttt
     2161 accattcgtt ccggcatatc cgccaaaaca ccgcgcaatg gactgccgat tttgaaaaac
     2221 aactcgactt ttccaaagcc gtttgggcgg cgcaatacgg cttgggcggc ggcagagggg
     2281 acaatgccaa ctcggattac agctatttcg caaaactgta cgcccccaaa atcctcgctt
     2341 ccaaccaagc caaaatcaca atgctgatcg aaaaccggtc gaaatataaa tttgcctatt
     2401 ggaacaatgc gtttcacttg ggcggcaacg accgcttccg cctgaacgcg ggcatacgct
     2461 acgacaaaaa cagcagcagc gcgaaagacg atccgaaata caccaccgcc atccgggggc
     2521 agattcccca tttgggttcg gaacgcgcgc acgcgggctt cagctacggc acggggttcg
     2581 actggcggtt taccaagcat ctgcacttgt tggcaaaata cagcaccggc ttccgcgcac
     2641 cgacttcgga cgaaacctgg ctgctgttcc cacaccccga tttctacttg aaagccaacc
     2701 caaacctgaa agcggaaaaa gccaaaaact gggaattggg tctggcgggc agcggcaaag
     2761 cgggcaactt caagctctcg ggcttcaaaa ccaaataccg cgactttatc gaattgacgt
     2821 atatgggcgt ttcgtcagac gataaaaaca accccagata cgccccgctt tcagacggta
     2881 cggcattggt cagctcgccc gtttggcaaa accaaaaccg ctccgccgcc tgggtgaaag
     2941 gcatagagtt taacggcacg tggaacctcg acagtatcgg tttgcccaaa gggctgcaca
     3001 ccggcctcaa cgtcagctac atcaagggca aggcaacgca aaacaacggc aaagaaacgc
     3061 ccatcaacgc gctttcgccg tggacggcgg tttacagcct gggctacgac gctccttcca
     3121 aacgttgggg catcaacgcc tacgccacgc gcaccgccgc caaaaagccg tccgacaccg
     3181 tccacagcaa cgacgacttg aacaacccgt ggccttatgc caaacacagc aaggcctata
     3241 cgctgttcga cctttccgcc tacctcaaca tcggcaaaca ggttacgctc cgcgccgccg
     3301 cgtacaacat taccaacaag cagtactaca cttgggaatc tttacgcagc atccgcgagt
     3361 tcggcacggt caaccgcgtt gacaacaaaa cccacgccgg catccaacgc tttacctcgc
     3421 cgggcaggag ctacaatttc accatcgaag cgaagttcta agctcctgat gtgccaatgc
     3481 cgtctgaaac ccgatgattt gggtttcaga cggcattttt tacggtcgca ccgccgcccc
     3541 ctttcaggct tgtaccaagc ccaatcccgc aactctccgt cattcccgca actctccgtc
     3601 attcccacgg aagtgggaat ctagaaacgc aaagctgcaa gaatttatcg gaaatgactg
     3661 aaactcaacg aacctggatt cccgcctgcg cgggaatgac gaagctatcc gcacagaaac
     3721 ctgcaccacg tcattcccac gaaagtggga atccagaacg taaaatctga agaaaccgtt
     3781 ttatccgata cgtttccgca ccgacagacc tagattcccg ctttcgcggg aatgacggcg
     3841 gaaaggttgc tgtttttccg ataaattcct gccgctcttc gtttttggga tggcgggaaa
     3901 taaaacaaaa gcgcgcgtat caaaaaacaa caatgcaaag aacggtgttg gcaattttaa
     3961 ggtttgcgcc ttagccgcac ttattcgcaa aaaaaccgca cggcgttgac cgtgcggctt
     4021 gttgtctgaa ggcttcagac ggcattgctt acatcatgcc gcccatacca cccatgccgc
     4081 ccatatcggg catagcaggt ttttcttcag ggatttcggc aatcatgcaa tcggtcgtca
     4141 gcatcaggcc ggcgatggac gcggcgtgtt gcagcgcgga acgggttact ttggcggggt
     4201 cgagtacgcc catttcgatc atgtcgccgt attcgccgga gcctgcgttg taaccgtagt
     4261 tgcctttgcc ttccaacact ttgttcacca ccacgctagg ttcgccgcct gcgttggcaa
     4321 cgatttggcg cagcggagac tcaacggcgc gcaagacgat ttgtacgcct gcgtcttggt
     4381 cggcattgcc ggtgtgcagg ttttccagag cggcacgggc gcgcaacagg gcgacaccgc
     4441 cgcctgcaac cacgccttct tcaacggctg cgcgggtagc gtgcagcgcg tcttccacgc
     4501 ggtctttttt ctctttcatt tcgacttcgg tcgcggcacc gactttgatc actgccacgc
     4561 ctcctgccaa tttggcaacg cgctcttgca gtttttcttt gtcgtaatcg ctggtcgcgg
     4621 tttcgatttg ttggcggatt tcggcaacac gcgcttcgat ttgggctgcg tcgccgaagc
     4681 cgtcgatgat ggtggtgttt tctttaccga tttcgatgcg tttggcttga cccaagtcgt
     4741 ccaaagtcgc tttttccaga gacaggccga cttcttcgga aatcaccacg ccgccggtca
     4801 ggatggcgat gtcttgcaac atcgctttgc ggcggtcgcc gaagccaggg gctttgacgg
     4861 caacggtttt caggatgcct cggatgttgt tcacgaccaa agtcgccaag gcttcgcctt
     4921 ctacgtcttc agcgataatc aacagcggac ggctggcttt tgccacttgt tccaaaacag
     4981 gcagcaagtc gcggatgttg ctgatttttt tgtcgaacaa caatacaaac ggattgtcca
     5041 aagcagcgat ttgtttttca gcgtcgttga tgaagtaagg agacaagtag ccgcggtcga
     5101 actgcatacc ttcaaccacg tccagctcgt tttccaaaga tttgccgtct tcaacggtaa
     5161 tcacgccttc tttgccgact ttttccatcg cttcggcgat aatcgcgccg acttgttcgt
     5221 cagagttggc ggaaatcgag ccgacttggg cgatttcttt agaagtgtcg caaggtttgg
     5281 cgatgttttt cagctcgtca accaaagcgg caacggcttt gtcgataccg cgtttcaggt
     5341 cggtcgggtt cataccggcg gtaacgtatt tcataccttc ggcaacgatg gattgcgcca
     5401 atacggtggc ggtagtcgta ccgtcgcccg ccacgtcgtt ggttttggac gcgacttctt
     5461 tcaccatttg cgcgcccata ttttcaaact tgtctttcaa ttcgatttct ttggcgacgg
     5521 ttacgccgtc tttggtgatg tgcgggccgc cgaatgcgcg gtcaaccacc acgttgcggc
     5581 ctttgggacc caaggttacg cgtacggcgt ttgccagaat gttcacgccg tttaccattt
     5641 tttgacggac ttcattgccg aactgtacgt cttttgctgc catttcaatt ctccaaaaat
     5701 cattaaaact gtctgataaa accgtttatg ccgtctgaag gcggtttgcc gtttcagacg
     5761 gcatcgtgtc cgtatttatt tttcaacgat gccgaagatg tcttcttcgc gcatcaccaa
     5821 cagctcttcg ccgtcggctt ttacggtttg gccgctgtat ttgccgaaga tgattttgtc
     5881 gccgactttg acatccagcg gacggcgcgc gccgtcttta ccgattttgc ccgcgcccac
     5941 ggcgatgact tcgcccatat cgggtttttc ggcggccgca cccggcaaaa cgatgcccga
     6001 tgcggttttc tcttcagctt ccaagcgttt gacgacgacg cggtcgtgta aaggacggat
     6061 ggtcatatct atgctccgat aaaatggttt gaaaacaatc atctgcccga acggttcgga
     6121 cagattaagg atgaaaaccg gacagccgtc aggcggctgc ccgtataagt cggcaaaatt
     6181 agggtgtgtg cgggcaaatt caagtgaggc ggaaaaaatt tatttccgcc gttttttata
     6241 gtggactaaa tttaaggggc tgtactagat tagcagatat gttaccctcg gaatataaag
     6301 ataacgcact gcaaat
//
LOCUS       DAWKOD010000113         6260 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000113 DAWKOD010000000
VERSION     DAWKOD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.113"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(391..825)
                     /locus_tag="WJK73_001570"
     CDS             complement(391..825)
                     /locus_tag="WJK73_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ7558034.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJK73_001571"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJK73_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ7558035.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WJK73_001572"
     CDS             complement(1556..2398)
                     /locus_tag="WJK73_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ7558036.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WJK73_001573"
     CDS             2653..3291
                     /locus_tag="WJK73_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ7558037.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WJK73_001574"
     CDS             complement(3743..4543)
                     /locus_tag="WJK73_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ7558038.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WJK73_001575"
     CDS             complement(4679..5182)
                     /locus_tag="WJK73_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558039.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WJK73_001576"
     CDS             complement(5519..6160)
                     /locus_tag="WJK73_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ7558040.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1664 a         1566 c         1307 g         1723 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttatacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcgaaataa agtggcacgc ccgatttttc actcataccg attaaataat
     5581 ccatcgtctt ccgagggaac gcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc
//
LOCUS       DAWKOD010000114         6237 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000114 DAWKOD010000000
VERSION     DAWKOD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.114"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            307..753
                     /gene="yciA"
                     /locus_tag="WJK73_001577"
     CDS             307..753
                     /gene="yciA"
                     /locus_tag="WJK73_001577"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ7558041.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(804..1625)
                     /locus_tag="WJK73_001578"
     CDS             complement(804..1625)
                     /locus_tag="WJK73_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7558042.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1744..2202
                     /locus_tag="WJK73_001579"
     CDS             1744..2202
                     /locus_tag="WJK73_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7558043.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2366..3481)
                     /gene="lst"
                     /locus_tag="WJK73_001580"
     CDS             complement(2366..3481)
                     /gene="lst"
                     /locus_tag="WJK73_001580"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ7558044.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3582..3686
                     /locus_tag="WJK73_001581"
                     /pseudo
     CDS             3582..3686
                     /locus_tag="WJK73_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3914..6139
                     /locus_tag="WJK73_001582"
     CDS             3914..6139
                     /locus_tag="WJK73_001582"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ7558045.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKERRAPVALK
                     AGEITDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRILAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDEDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSVTFNQALNA
                     L"
BASE COUNT         1666 a         1834 c         1387 g         1350 t
ORIGIN      
        1 aatcttgaaa tccgtccgac aacaccgtaa tcttaaaacc cgtcattccc gcgcaggcgg
       61 gaatctagac ctgtccgcac agaaacttat cggataaaaa cagttgccca aaccccgcgt
      121 tctagattcc cactttcgtg ggaatggcgg ttcagttgct acggccccgc cctacaatac
      181 gccttgcgaa tctgtcccac gcccgccctt gtatgcctca aatgggttaa actacgcact
      241 tcccgacttc ccgtttcggg caggggtgca ggttcgattt atctttcaat aaaacaagga
      301 aactttatgc agcacgaaga aggcaaccgc caacgccctc aaggcgaact gctcctgcgt
      361 accgtcgcta tgccgcgcga taccaatccc aaccaagaca ttttcggcgg ctggattatg
      421 tcgcagatgg atttgggcgg cggcatattg gcagcggaaa tcgcacaagg acgcatcgtt
      481 accgtcgccg ttcaggaaat gaacttcatc cgcccggtca aggtcggcaa cgtcgtctgc
      541 tgctacgggc attgcgtccg cgtgggcaat acttccctcc agcttaaagt cgaagtctgg
      601 gtgaaaactt tgatgaacga ttgcgttacc gaagaccgcc acctcgtaac cgaagccgtg
      661 ttcacttatg tcgccatcga tgcggaaggc aatccgcgcc cgattccgaa agaaggcaac
      721 cccatattgg cgggcttatt gcctactccg taaaaatgcc cgtaaaaatg ccgtctgaaa
      781 ctgtcttcag acggcattcc gcatcatttg aaccaagatc tgatgcggga aaacagcgac
      841 atactctgtt tttcaaagtc ttcccgcact tccggcacgg ctttttttat caccttcatt
      901 cctgcaaaag tctgcgccac gggcataatt tcaatcgtgt tcacattcat atgcgccggc
      961 cggcggtaca gccacaatgc ggtttccgca atatcttcgg ggcggataaa ttccacaccc
     1021 tcatacacgc ctgctgcctt ctcgtcatcg cctttgaagc gcacgttgga aaactccgta
     1081 ttgccgcaca aacccggctc gatattggta acgcggatgt tcttatccgc caactccgcg
     1141 cgcaaattca ggctgaactg gcgcacaaac gccttggtcg ccccgtaaac gttgctgccg
     1201 gcataagcat aattgcctgc aatcgaaccc aaattcatca cataaccgcc gccccgagcc
     1261 accatattcg gcaaaatctt gcgcgttaga aacgtcaaac ccaaaacatt ggtttgaatc
     1321 attgtttccc aatcttcaaa atccgccttg tccgccgtgt ccagacccaa agccagcccg
     1381 gcattgttga tgaggcagtc gatgtcggaa aattcatcag ggatgccgtt taaggcgttt
     1441 tccaccgact cgcggcgcga cacgtccatt tccaaagggt aaaacaaagc acccaattca
     1501 tccgccaagg cctgaagcct gtccgcacga cgcgccgcac cgataacgcg gtatcccgcc
     1561 ccgacaaacg cgcggcacat cgcttcgccg aatcctgccg aagcaccggt aattaaaaca
     1621 gccattgttt ttcctttctt tctcgtttcc caaacctgtt tcaggtatca taacgctctt
     1681 cagacggcat cgcgccggct gaaaatgaca acaaccatac caccacgaca ggacacattc
     1741 aatatgaaaa cccaaatcag ccttgctgct gccgccatca cactgctcct ttccgcctgc
     1801 ggaggcagcg gaataccttc ccaacccaaa ggcgaaattt ccgaaaaccg caccgccgcg
     1861 ttcaaatcta tgatgcccga cttctcgcgt atgggcaaaa tggtcaaagg cgaagagcct
     1921 tacgatgtcg aaaaattcaa acaggcggca gcggcgtttg ccgacagcag caaaaaaccg
     1981 tttacgcttt ttgagtccga cccgcaaggc aacgggcgcg cactgcccgc cgtttggtcg
     2041 gatggtgcaa aatttgaagc cgaaaaaaca aaattcgccg ccgccgtcga aaaactcaac
     2101 gccgccgccc aaaccggcaa actggacgaa atcaaagccg cctacggcga aaccggcgca
     2161 agctgcaaat cctgccacga cagtttccgc gcgcccgaat aacatccaaa acaaaacaat
     2221 gccgtctgaa gccttcagac ggcattttct ttgccatttc accaagcgca aagcaatcaa
     2281 aaattatcgt taattcaaaa aatacgccat atcctgattt ttgccttact tactccgcca
     2341 ttttctattt tgatttgcta tgcgattaat ctttatcgtc aaatgtcaaa atcgggatgc
     2401 cggattgggt aaacagggca tacaccggct tgagccaata atcttccgga agggaagccg
     2461 gtttcaatgc gtaaacgtgc acattgggaa aatccttcat cgtcaacgcc gcgccgctga
     2521 aaaaggtata aatttcatac ctcgtatgcg ggtttttctt aatctcgcgc aaaatatagt
     2581 cttcgatgac atagggcgaa tttaatgtgg ttacgccgga aagcccgtag gtttggcggg
     2641 ggtgcggtgc gacatattgt atgttgaagt tttttgccgc cttttccgaa atttccttca
     2701 tctccttgtc gggcgaaccc aaaagtatcc gcaccgtgcc gcccgtttcg tcccccgcct
     2761 tcagttcgga cgcatcgaac agcggcaggt aagtcatctt gcggcggccg tcgtccataa
     2821 tgtttttcaa acccttgaat atcgtgtaat gctcgtcgga agcattacgg gttttggcga
     2881 tgctccaatc tccgatcatc atccgggcaa aattccgctt gatcgtcccg tttacagaaa
     2941 actcatcgcc caaatagctg ctgctttgaa ttaaattgcc tgtcccgtcg tcaaaggttt
     3001 tgatttccgc atccgggtaa gtgctcaaaa aggcggcaat gctgactttt tccaaacttg
     3061 ccaaataaat ccgcttgact ttcggcagca gcatcgactt taccttcagc tccgccatcg
     3121 tcggaatgaa attaaacgat ttgttcaaac cgtagggcag gtggaaaaaa tacgcccgct
     3181 ccgccttatc ctttatctga ttgaaataat aatcgtattt ttcattcctg ttttcagaca
     3241 tcagcaccac ataaaaccgc tcccccggat gctgcgccat aatcctttcc gccaccttca
     3301 tctgcaatat ggtatagcag aaaatcagat tgaccggttc cccctcttca ttgaagagtt
     3361 tgtccttcag cagggaaacc gcattccttt ccccctgatt tacccggtca aatgtataaa
     3421 atatcccgaa acaaaaaaca atcaaacaca acacggtcaa acaagccttt ttcaagccca
     3481 tatccctaaa actccattcc gataaattga acatacgccc cgagtgtccc gggacggaaa
     3541 aatgcggaat tttagcagat attccgacag ttaccgaata ttatagtggt ttaaatttaa
     3601 atcaggacaa ggcgacgaag ccgcagacag tacaagcagt acgacaaggt gaggcaacgc
     3661 tgtactggtt taaatttaaa ccactataaa atcaatttca ccgaaaatat gccgccgcac
     3721 atcaggcaca gcacctccga ccgccaccaa acgcgggaaa accatttccc ccttcctttc
     3781 acgctgccgc gttcaaacac gccaagatcg aacaaagcgc gaacaaagcc gccaaaatat
     3841 gccataatag ccgcgcacat tccccgcgca atgtgttcca aaagaaaaaa ccaacctaca
     3901 agagaataat cctatgactc aaaaatccac cattgtttat acccataccg acgaagcccc
     3961 cgcgctggcg acccaatcgc tgctgccgat tgtgcaggct tttgcccgcc acgccgatat
     4021 tgatgtcaaa actgccgaca tttccctgtc cggccgtatt ttggcggcat tccccgaata
     4081 tctgaccgaa gcgcagcgcg tacccgacgc gcttgccgaa ttgggcgaac tggtgaaaca
     4141 gcccgatgca aacgtaatca aactgccgaa catcagcgcg tccgtacctc aactgaccgc
     4201 cgcgattaaa gaattgcagt ctaaaggctt tgccgttccc gactatcctg ccgacccgca
     4261 aaccgatgaa gaaaaagccg tacgcgaacg ctacgaccgc atcaaaggca gcgcggtcaa
     4321 ccctgtcctg cgtgaaggca actccgaccg ccgcgccccc aaagcggtga aaaactttgc
     4381 caaaaaaaat ccgcacagca tgggcgcgtg gacaaaagac tccaaaaccc acgttgccac
     4441 catgcaaagc ggcgactttt tccataacga acaatccgtt accgtacccg atgcaacttc
     4501 cgtatccatc gtgtttaccg acaaacaagg caacaaaaaa gagcggcgcg cgcccgtcgc
     4561 cctgaaagcg ggcgaaatca ccgacgcgac cgtgatgagc aaaaaagccc tgctcgcctt
     4621 ccttgccgaa caagtgaaag acgcaaaagc taaaggcgta ttgttctcgc tgcatatgaa
     4681 agccacgatg atgaaagtgt ccgacccgat tatcttcgga cacgccgtca aagtgttctt
     4741 cgcgcctgta tttgaaaaat tcggcggcaa actggctgcc gcaggcgtca acgtcaacaa
     4801 cggcttcggc aacctgcttg ccaatctaga caaactggat gcggacaccc gcgctgccgt
     4861 tgaagctgaa atcgccgccg tttacgctgc caaccctgat ttggcaatgg ttgattccga
     4921 caaaggcatt accaacctgc acgttcccag cgatgtcatc gtcgatgcct ctatgcctgc
     4981 gatgattcgc aattccggcc gtatgtggga caaaaacggc aaagcgcaag acaccaaagc
     5041 cgtgattccc gacagcagct atgccggcgt ttaccaagca accatcgact tctgccgcga
     5101 acacggcgcg ttcgatccga caacaatggg tactgttccc aatgtcggtt tgatggcgca
     5161 agcagcggaa gaatacggtt cacacaacaa aacctttgaa atcgaagccg acggtcaggt
     5221 tcaagtcatt gatgcggaag gaaaagtcct gatgcagcac gacgttgaag caggcgacat
     5281 ctggcgtatg tgccaaacca aagacgctcc ggtcaaagac tgggtgcaac tcgccgtcaa
     5341 ccgcgcccgc ctgagcaaca cgcctgccgt gttctggctc gatgaaaacc gtccgcacga
     5401 caagagcctg ctcgccaagg ttaaagccta ccttgccgaa ctggatacca atggcctcga
     5461 catccgcatc ctcgctcctg aagaagccgc caagttcagc ttgggtcgtc tgaaaaacgg
     5521 cgaagacacc atctccgtaa ccggtaatgt cttgcgcgac tacctgaccg acttgttccc
     5581 aattttggaa ctcggcacaa gcgcgaaaat gctgtctatc gttccattga tgaacggcgg
     5641 cggtatgttt gaaaccggcg cgggcggttc tgcaccgaaa catgttcaac aattcctcga
     5701 agaaaaccat ttgcgctggg actcgctggg cgaattcctc gcactcgccg tatcgtttga
     5761 acatttggcg caaaaaaccg gcaatgccaa agcccaagtc ctcgccgaca ctttggatgc
     5821 agccaccgaa aaactgctgt tgaacgacaa atcgcctaaa cgtaaagccg gcgaactcga
     5881 caaccggggc agccatttct accttaccct ctactgggcg caagaattgg cggcgcaaga
     5941 cgaagatgcc gaactgaaag ccgcatttgc gccattggca gccgctttga ctgccgacga
     6001 agccaaaatc gttgaagaac tttctgccgt acaaggcaaa gcggtcgaca tcggcggcta
     6061 ctacgccgcc aatcctgaaa aagcggcaca agcgatgcgc ccgagcgtaa cctttaatca
     6121 ggcactaaac gccttataag cacaaaggta aaaatgccgt ctgaacattc gtcgttcaga
     6181 cggcattagt tatccctatc tgcctgatta tgattaaacg ccgcacccat tggatat
//
LOCUS       DAWKOD010000115         6140 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000115 DAWKOD010000000
VERSION     DAWKOD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.115"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(117..2123)
                     /locus_tag="WJK73_001583"
     CDS             complement(117..2123)
                     /locus_tag="WJK73_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ7558046.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2412..4439
                     /locus_tag="WJK73_001584"
     CDS             2412..4439
                     /locus_tag="WJK73_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ7558047.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4531..6084)
                     /locus_tag="WJK73_001585"
     CDS             complement(4531..6084)
                     /locus_tag="WJK73_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ7558048.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1264 a         1437 c         1870 g         1569 t
ORIGIN      
        1 ttaaaatgcc gtctgaacca cgtttcggtt cggacggcat ttttgcatcc ttttatgggg
       61 atttcccgca tcagggcggt attcggggcg aatcccgctt cagacggcat tgccggtcaa
      121 ggcttgtccg aaggaggatg tccctgttct ttggcgggcg ggaggaaaag tatgcaggga
      181 ttgcttttaa tctgattcca aaaactctta atcagattgc ggatgctgcc gattttcgtg
      241 ccgcgcgagc gcagggcgac gaagagggcg aggctgaagc tgacgcagag gttgaccagg
      301 ccgatggcga ggacgctgaa aatgccgagt acgaacgtgc ccaaaccgac gttgccgctg
      361 acggcggcat agccgaggtt ggcggaggaa aaggcgacgt ggcggatgtc cagcggcagc
      421 ccgaggaggt gtccgaaata gccggtcata cccaagagca tcccgaagat gaagttgcct
      481 gccaacgagc cgtagtgttt gtggatgtag gcggcgagga cgcggcgggg cccggggcgc
      541 atgattttac gcagcaaggg gttgaagggc aggcgttggc gcaggttgag gtagtcggcg
      601 cggttgtcga aaaaacctgc gatgatgccg gagcagaaca gccacagacc tgcaatggcg
      661 gcataccaca gcgtcgggta agcgatgatg tctatggatt tgaactggta ggcggcggtg
      721 tgggcatcga gtatgggctg ccggtacaga tgggcatagc cgaacgatat ggcgcacgcc
      781 aaaaggatgg cgatggaaac gttgccgaaa acggcgacac tttgggaacg gcatacgtcg
      841 atgaggagct tggcgagttt gttgtccacc gctttgccgc cttcgttgag atcgacctgt
      901 tcggcaaagc tggcggcagt catcgcgggc tgtttggtgg cgacggtgca gtgcagcata
      961 tggatgatca taaagccgat gccgtagttg aacccagccg acaaggaagt caggaagggg
     1021 ctgaggccga gtgtgccgat gcggattttg agcagcgcca tcagggcgat gatgatgccg
     1081 ccgcctgccg ccgagtagaa cattttgaaa tattctttgc ggttgcgggt aatgtagtgt
     1141 tcgccgtggt ggcttttgtt ttcggtaatg ctgcgggcga gcatacggat gctgctgcgg
     1201 cgcaggtggc gggtgctgta ttgttcgacc gccgcgtaga tgagggagtt catcagcgcg
     1261 atggtcaggc ggttgctgtt gccggcgccg gtttggatgt cggtgagcag cttcagacgg
     1321 tctacggtct gccggagccg ttcgagcagg tgggcgactt tgaccgacga accggagccg
     1381 gcgcccgtcc ctttgcggcg caaatagtca atctggctga aacattggtc gaacatcact
     1441 tcgaggtggg cggtgtcgta aggcgtggtg ccgttgcggt agtgttcgac cagtttggcg
     1501 gtttcgcgtt ggagggcgac gaaggaagaa tcggcttcca gcaggcgcgg ggcgatgcgg
     1561 atgaggtcgg gttcgatggc ttcggatgcc gtccagacag acagcatttc gatggcgcgc
     1621 aaacgcgcat cggcaaggcg gatgcccgcc gtctgcaatt tggacggctc ggcgtggctc
     1681 cggatgagtt cgtataaaac cagccattgg cggatattga gcgtttgcag ccatttgtcg
     1741 tcgttttcgg aatggaaaag ataaaggaag acttcgccca agttggcaaa atctttatat
     1801 gacgggctga agcgttcgta gatgcgtatg cccatttccc gggcaaagct gtggcgcgag
     1861 aagatgccga gtttgatgag tgccggataa atgtgtattt gcgcgagcca gatgtaaaaa
     1921 caccggctga agcgggacag caattccctg tcttgtttga atgtgtcgat aatcaggtcg
     1981 aaacgttccc ccgcacattt tttgccgcca cggcgcaaaa atttaatcag tgcgttgagg
     2041 acgttgacaa aatcggtatg tcccaagctt tccgaaagca gggggcgcag gttttgaggg
     2101 gtaattttct tcatatcagc cattgtaccg catcggcaat ccggcgttaa acgccccgat
     2161 ggcggggagg gcgcgcctgc ggaaggcggc gcattttccg ccggtttgaa atcaaactat
     2221 attaatgata taataaatat cgataaatca atctgataga ataatccgta ttgattttta
     2281 attttttcga ttataacaat ttcggccggt ttgtgcattt cgtcatccgg aagccggtat
     2341 tttgccggca ggttttgacc gctttgcccg atgaccgggt ttgtctgttt ttaacaaagg
     2401 agtgagaagg tttgtccctg tctgaattta tagaacgccg aacgtcattt aatctgatgg
     2461 ttattttgac gactttgttt tttgtgtgtg ttttggtggt attggtttta accgtgccgg
     2521 atcaggtgca gatgtggctc gatcgggcaa aagaaatcat ttttaccgag ttcagctggt
     2581 tttatgtttt aacgttttcc atttttctgg gtttcctgct gatactctcg gtcagcagtt
     2641 tgggaaacat caggctcgga cgggatgaag atgtgccgga attcggcttc ctgtcgtggc
     2701 tggcgatgct gtttgcggcc gggatgggcg tgggtctgat gtttttcggc gtggcagagc
     2761 cgttgatgca ttatttttcg gacattacgg ccggcacgcc ggaacacagg cagcagcagg
     2821 cattgctgca cacggtgttc cattggggcg ttcacgcttg gtcggtgtac ggtacgattg
     2881 cattggcttt ggcttatttc ggtttccgct acaagctgcc gcttgccctg cgttcttgtt
     2941 tttaccccct gttgaaagaa aaaatttccg gaaggttcgg cgatgccatt gatattatgg
     3001 cgttgcttgc tacttttttc ggcatcatca ccacattggg gttcggggct tcgcaactgg
     3061 gcgccggatt gcaggaaatg ggctggattg ccgaaaacag cttcagcgtg caggttttga
     3121 ttatcgccgc cgtcatgtcc ctcgccgtcg tttcggcaat atccggcgtg gggaagggcg
     3181 tgaaggtgtt gagcgagttg aacctgagcc ttgcgttttt gctgctgttt tttgttttgg
     3241 cggcgggtcc cactgtttac ctgttgtcgg cattcggcga caacataggg aactacctcg
     3301 gaaatctggt gcgcctcagt tttaaaactt atgcgtacga acgggaacac aagccgtggt
     3361 ttgaatcttg gacggtgctt tattgggcgt ggtggtgttc ttgggcgccg tttgtgggtt
     3421 tgtttatcgc gcgcatttca aaggggcgca ccatccgcga gtttgtcttc ggggttttgc
     3481 tcatccccgg cctgttcggc gttttgtggt ttaccgtctt cggcaatacg gcgatttggc
     3541 tgaatgacgg ggttgcgggg ggaatgctcg aaaagatgac ctcctctccg gaaacgctgc
     3601 tttttaaatt ctttaattac ctccccctgc ccgaattgac gagcatcgtc agcctgctgg
     3661 tcatttctct gttttttgta acttctgccg attccgggat ttatgtcctg aacaatatta
     3721 cctctcggga caaaggcttg agcgcgccac ggtggcaggc ggttatgtgg ggcgtgctga
     3781 tgtctgccgt tgccgttttg ctgatgcgct cgggcggact cggcaacctg cagtctatga
     3841 ccctgattgt ttccctgccg tttgccctgc tgatgctgat aatgtgtttc agcctgtgga
     3901 aaggcttgag tgcggataag aaatattttg agacccgggt taaccctacc agtgtatttt
     3961 ggacgggcgg caagtggaaa gaacggctgg tgcagataat gagccagacg caggagcagg
     4021 atattttaaa attcctcaaa cataccgcat cgcccgctat gcacgagttg caacgggagc
     4081 tttcggaaga atacggcttg agcgtccggg tcgataagat gtttcatcag gacgagcccg
     4141 caatcgagtt cgtcattcgg aaagagacga tgcgcgattt tatgtacggg attaagtctg
     4201 tcgggcagga tgtatccgac cagttgatta acgacggcaa gctgccgcat atccggcatc
     4261 agacaactta caaaccctac gcttattttt tcgacgggcg cgtcgggtac gatgtgcagt
     4321 atatgaacaa ggacgagctg attgccgaca ttttgaaaaa ctacgaacgt tatttgatgt
     4381 tgttggatga tgtcggtcag gaactgatgg cgcacgagca ggtggaattg gcagagtaag
     4441 tgccttatct ctggcgtttg ccaaaaaaaa tgcggtgaat cagattcacc gcattttttt
     4501 tgccggacgg ggcttcggac ggcacggcgt tcatttgttt ccgtcaaact gctccttcaa
     4561 cacccgtttc aataccttgc ccgtagcgtt gcgcggcagc ccgtctttaa agtggatttg
     4621 tttggggatt ttgaaatttg ccagcacggt acgcaggtgg cggcggattt cgttctcgcc
     4681 caaatccata ccttccttca attggacgaa ggcgacgatt tcctcgtcgg cataacggtc
     4741 tttcacgccg atgacggcgg cggcttcgac ggcatcgagt ttgtagattt cttcttcgat
     4801 ttcgcgcgga tagacgtttt gacctttgga aataatcaaa tcttttttgc ggtcgacgat
     4861 aaagataaag ccgtcttcgt ctatggtaac gaaatcgccc gttttcaacc agccgttgac
     4921 gatggtttca tcggtggcgg caggcatatt gaggtagccc cgcatcaccg aaccgcccct
     4981 gacgatcagt tcgcccactt cgccgcgcgg cacttcgacc aattcttcat cgacggcttt
     5041 ggcttccaaa ccgggcaggg ggatgccgac gctgcgggct ttttgcctct cgggcgtatt
     5101 gacggcgacg acgggcgagg cttcgctcag tccgtagcct tccagcaatt tggcgcgggg
     5161 gaacttggct ttgaaatcga ggatggtttg ttccgccaaa ggtgctccgc cgctgataaa
     5221 caggcggatg cggttgaacc atctgaaata ccaagggatt ttcgtcttgc tcatcgcggt
     5281 gtaaatcgcg ggcacgccca aaaacacggt cgcgcgtttg agcagtgcct gtttcaaaac
     5341 gttggaaaag gggaaaacgg atttgaccaa aataatcgaa cacgccatat aaatcggcag
     5401 cagcaccata gccgtcagcg tgaagctgtg gaacatcggc aggaaaacga taaagcggtc
     5461 gcgcttggag attttaaaga tgcgttcgat gccgttcagg ttggcgaaca ggttggcgta
     5521 gctgattagc gcacctttgg gatgccccgt cgtgccggag gtgtagatga tgtgtgccaa
     5581 atcatttatc cggggttggc ggcccaagtc gggtttttcg gggaagcggc gcacgtcttc
     5641 aaaaaaggca tcgccttccg ccatttcgcc gtccggacgg ctttggcccg tccaaatgat
     5701 tttttcgacg ggcgtttgcg ccttcaagcc cgccaattct tttgacaggc cggccgaggc
     5761 gaacaggaag cgcgccttgc agtcgttcag gatatacgcg tattcgctgt ttttcaaaaa
     5821 tgtgttcatc ggtacggcaa ccgcgccgat ggcggatacg gcgaaatagg cggtaataaa
     5881 ttccgtggaa ttggaaaccg ccagcgcgac cgtgtcgccg aacttcacgc cgatattttg
     5941 cagatacgcc gcaacggctt cggcttcctg cttgagcgcg cggtaggcgg ttttttcctt
     6001 gccgtcgaac actgccgtgc cgttgccgtt tttgcggcag gcggcggtca gcatttcgta
     6061 gaaattggca taagtccggt tcatgaaact tcctttatgg ttgcggacgg cgaacgccct
     6121 catttgaaaa caatatgccg
//
LOCUS       DAWKOD010000116         6133 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000116 DAWKOD010000000
VERSION     DAWKOD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.116"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            295..993
                     /locus_tag="WJK73_001586"
     CDS             295..993
                     /locus_tag="WJK73_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7558049.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            complement(1050..2054)
                     /locus_tag="WJK73_001587"
     CDS             complement(1050..2054)
                     /locus_tag="WJK73_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ7558050.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2124..4532
                     /locus_tag="WJK73_001588"
     CDS             2124..4532
                     /locus_tag="WJK73_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ7558051.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4637..5662
                     /locus_tag="WJK73_001589"
     CDS             4637..5662
                     /locus_tag="WJK73_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7558052.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVRSLK
                     PGQVSAPVRTQFGWHIIKLNEVREAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
BASE COUNT         1529 a         1864 c         1603 g         1137 t
ORIGIN      
        1 gaaaggggtg taacccttcc gaaccagggc aacgcatagg gcggtgcttt atgtgccgtc
       61 ctgtgtgttg gaacatcatt agttccccaa cagccgtatc cgtctggggc gacggcgcgt
      121 attgtacgac agaaaccgct tcagacggca tcgggtttga tgccgtccga agcggtttcc
      181 ccaaacgaaa acgtcctttt ccggaggcaa actgtatcaa cacgcggaaa tgcaataaaa
      241 tagccggatt ccgattgatt tccaacatct gtttccaaca tcacggagag ccgtatgaaa
      301 tccaaacacc tcgccctcgg cgttgccgcc ctgttcgcac ttgccgcgtg cgacagcaaa
      361 gtccaaacca gcgtccccgc cgacagcgcg cctgccgctt cggcagccgc cgccccggca
      421 gggctggtcg aagggcaaaa ctatactgtc cttgccaacc cgattcccca acagcaggca
      481 ggcaaagtcg aagtccttga gtttttcggc tatttctgtc cgcactgcgc ccacctcgaa
      541 cctgttttaa gcaaacacgc caagtctttt aaagacgata tgtacctgcg taccgaacac
      601 gtcgtctggc agaaagaaat gctgacgctc gcacgcctcg ccgccgccgt cgatatggct
      661 gccgccgaca gcaaagatgt ggcgaacagc catattttcg atgcgatggt caaccaaaaa
      721 atcaagctgc aagagccgga agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac
      781 ggcaaaaaag tccttgccgc ttacgaatct cccgaaagcc aggcgcgcgc cgacaaaatg
      841 caggagctga ccgaaacctt ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa
      901 tataaagtcg aatttgccga ctgggagtcc ggtatgaaca ccatcgacct tttggcggac
      961 aaagtacgcg aagaacaaaa agccgcgcac taagcccgtt tggaaaatgc cgtctgaaac
     1021 ttggttttca gacggcattt tgattgggtt taaaacgtaa agcccgtttc cagttcttca
     1081 tcgccgacca gttcgaccaa gagcgcgtag agcggggcga gttcggcata acggcgcgat
     1141 acgcggcgca ggtaattgag gaaacgcggg atttcgggac ggtatttgtc tttgccgtcg
     1201 cggtagtaca ggcgtgcgaa gatgcctgca accttcaagt gccgctgcac gcccatccat
     1261 tcgaaccagc ggtaaaactc gtcaaacgcc tcggggacgg gcaagccggt agcccgcgcc
     1321 ttttcccagt agcggataac caagtccaag acaaattctt cttcccattc gataaaggca
     1381 tcgcgcaaca gcgacaccaa atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct
     1441 aaaacgcccg gtctgccgcg cgtcagcatc aggttgcgga cgataaagtc gcggtgcaca
     1501 tagactttgg gctgcgccaa caggggcggc agcagcgtat cgacggtttg ctgccaaagt
     1561 tggcgttgtt tgaatgttaa ttcgcgcccc aattcttttg cgacaaacca ttccgggaac
     1621 aggttgattt cgcgcaacat cgtttcacgg tcatattcgg gcaaaacccc tttacggctc
     1681 gccttctgca attcgaccaa ctcgccgatg gcctccaaaa gcagggcttt gtgcgccgtt
     1741 tcgccctgtt cctgaagcat cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc
     1801 acaaacccca aatccgtgtc cgcgtgcaat atctgcggca cattgaccat gtcaaacagt
     1861 ttctgcactt ttaaataagg cgcgacactc atcttgtcgg gcggcgcgtc catacagacg
     1921 acgctgccgc cgtctgaaaa cgttgcacgg aaatagcggc ggaaatcagc atccgccgcc
     1981 gcgaaggaca gatcgaagtc ccgttcggga tagatggtct gaagccaatt tttcagttcg
     2041 gtttgccgtt gcataacagt actaaagcat ttcaggttac aataaacgct attctaactg
     2101 gcaaaccgac ttgaggggcg attttggctc gtttattttc actcaaacca ctggtgctgg
     2161 cattgggctt ctgtttcggc acgcattgcg ccgccgccga tgccgttgcg gcggaggaaa
     2221 cggacaatcc gaccgccgga ggaagcgttc ggagcgtgtc cgaacccata cagcctacca
     2281 gcctgagcct cggttcgacc tgcctgtttt gcagtaacga aagcggcagc cccgagagaa
     2341 ccgaagccgc cgtccaaggc agcggcgaag catccatccc cgaagactat acgcgcattg
     2401 ttgccgacag gatggaagga cagtcgcagg tgcaggtgcg tgccgaaggc aacgtcgtcg
     2461 tcgaacgcaa tcggacgacc ctcaatgccg attgggcgga ttacgaccag tcgggcgaca
     2521 ccgttaccgc aggcgaccgg ttcgccctcc aacaggacgg tacgctgatt cggggcgaaa
     2581 ccctgaccta caatctcgag cagcagaccg gggaagcgca caacgtccgt atggaaaccg
     2641 aacacggcgg acggcggctg caaagcgtca gccgcaccgc cgaaatgttg ggcgaagggc
     2701 attacaaact gacggaaacc caattcaaca cctgttccgc cggcgatgcc ggctggtatg
     2761 tcaaggccgc ttccgtcgaa gccgatcggg aaaaaggcat aggcgttgcc aaacacgccg
     2821 ccttcgtgtt cggcggcgtt cccattttct acaccccttg ggcggacttc ccgcttgacg
     2881 gcaaccgcaa aagcggcctg ctcgttccct cactgtccgc cggttcggac ggcgtttccc
     2941 tttccgttcc ctattatttc aaccttgccc ccaatctcga tgccacgttc gcgcccggcg
     3001 tgatcggcga acgcggcgcg gtctttgacg ggcaggtacg ctacctgcgg ccggattatg
     3061 ccggccagtc cgacctgacc tggctgccgc acgacaagaa aagcggcagg aataaccgct
     3121 atcaggcgaa atggcagcac cggcacgaca tttccgacac gcttcaggcg ggtgtcgatt
     3181 tcaaccaagt ctccgacagc ggctactacc gcgactttta cggcaacaaa gaaatcgccg
     3241 gcaacgtcaa cctcaaccgc cgtgtatggc tggattatgg cggcagggcg gcgggcggca
     3301 gcctgaatgc cggcctttcg gttctgaaat accagacgct ggcaaaccaa agcggctaca
     3361 aagacaaacc gtatgccctg atgccgcgcc tttccgccga ttggcgcaaa aacaccggca
     3421 gggcgcaaat cggcgtgtcc gcccaattta cccgcttcag ccacgacagc cgccaagacg
     3481 gcagccgcct cgtcgtctat cccgacatca aatgggattt cagcaacagc tggggttacg
     3541 tccgtcccaa actcggactg cacgccacct attacagcct caaccgcttc ggcagccaag
     3601 aagcccgacg cgtcagccgc actctgccca tcgtcaacat cgacagcggc atgaccttcg
     3661 aacgcaatac gcggatgttc ggcggcggag tcctgcaaac cctcgagccg cgcctgttct
     3721 acaactatat tcctgccaaa tcccaaaacg acctgcccaa tttcgattcg tcggaaagca
     3781 gcttcggcta cgggcagctt tttcgtgaaa acctctatta cggcaacgac aggattaaca
     3841 ccgcaaacag cctttccgcc gccgtgcaaa gccgtatttt ggacggcgcg acgggggaag
     3901 agcgtttccg cgccggcatc gggcagaaat tctacttcaa aaacgacgca gtcatgcttg
     3961 acggcagtgt cggcaaaaaa ccgcgcagcc gttccgactg ggtggcattc gcctccagcg
     4021 gcatcggcag ccgcttcatc ctcgacagca gcatccacta caaccaaaac gacaaacgcg
     4081 ccgagaacta cgccgtcggt gcaagctacc gtcccgcaca gggcaaagtg ctgaacgccc
     4141 gctacaaata cgggcgcaac gaaaaaatct acctgaagtc cgacggttcc tatttttacg
     4201 acaaactcag ccagctcgac ctgtccgcac aatggccgct gacgcgcaac ctgtcggccg
     4261 tcgtccgtta caactacggt tttgaagcca aaaaaccgat agaggtgctg gcgggtgcgg
     4321 aatacaaaag cagttgcggc tgctggggcg cgggcgtgta cgcccaacgc tacgttaccg
     4381 gcgaaaacac ctacaaaaac gctgtctttt tctcacttca gttgaaagac ctcagcagtg
     4441 tcggcagaaa ccccgcagac aggatggatg tcgccgttcc cggctatatc cccgcccact
     4501 ctctttccgc cggacgcaac aaacggccct gaccgtcgga aacccggcag gagcaccgtt
     4561 cccgcacaag acggcattcc accgacaacc ccaaacccgc catcaaaggc aggattcaaa
     4621 cgataaggaa agaatgatga aaatcaaagc cctgatgatt gccgccgcat tgctggcagc
     4681 agccgatgtc cacgccgcac cgcaaaaggc aaaaaccgca tccgccaaag ctgccaaagc
     4741 tgccaaagct gccaaagttg ccaaagttgc cgccacggcg caaaaagaag ccgcacccgc
     4801 acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg acaacgaagt
     4861 catcacgcgc cgccggcttg ccgaagccgt tgccgaagcc aaagccaacc tgcccaaaga
     4921 cgcgcagata agcgaatccg agctgtcccg acaggtgctg atgcagcttg tcaaccaatc
     4981 cctgattgta caggcgggca aacgccgcaa cattcaagca agcgaagcgg aaatcgatgc
     5041 cgtcgtcgca aaaaattccg ccctcaaaaa cctcagcccc gcccaacgcc gcgattttgc
     5101 cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcagaaca gccgcgtgag
     5161 cgaagctgaa atcgatgcct tcctcgagca ggcgcaaaaa caaggcatca ccctgcccga
     5221 aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg acagcgaaaa
     5281 cgccgccgtc ggcgcggaaa gcaccatccg caaaatctac ggagaggccc gcagcggcac
     5341 agacttttcc agcctagcgc gccaatattc gcaagacgcg agcgcgggca acggcggcga
     5401 cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg tccgcagcct
     5461 caaacccggc caagtcagcg cgcccgtacg cacccagttc ggctggcaca tcatcaagct
     5521 gaacgaagta cgcgaggccg gcacacctca ggaacgtatc cgcaattccg tgcggcaata
     5581 catcttccaa caaaaagccg aacaggcaac cgtcaacctg ttgcgtgacc tgcattccgg
     5641 cgcgtatgtc gacatccgct aaggcggttt gaagcaaaaa gccataccga tcggcaaaaa
     5701 tccgggcggt atggcttttt ggatttcgag ttacttttac accgtcattc atcattcccg
     5761 cgaaagcggg aatctagaaa cgaaaagtaa caggaattta tcgggaatgg ctggagttta
     5821 aaggactgga ttcccgccgt cgcgggaatg acgggatttt gggttgtggt aatttatcgg
     5881 aaaaacaaaa aaacctatgc cgtcattccc gagcaggcgg gaatccggtt atttaaaact
     5941 gcagaaattt atccgaagca acaacaatct ttccatcgtc attcccgcgt aggcgggaat
     6001 ctagcaacga aaagtaacag gaatttatcg ggaaaacaga aacctctatg ccgtcattcc
     6061 cgcgaaagcg ggaatctagg acgtagaatc taaagcaacc gttttatccg ataagtttct
     6121 gtgccgaagg gtc
//
LOCUS       DAWKOD010000117         6117 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000117 DAWKOD010000000
VERSION     DAWKOD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.117"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(151..2079)
                     /gene="dnaK"
                     /locus_tag="WJK73_001590"
     CDS             complement(151..2079)
                     /gene="dnaK"
                     /locus_tag="WJK73_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ7558053.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2287..2559)
                     /locus_tag="WJK73_001591"
     CDS             complement(2287..2559)
                     /locus_tag="WJK73_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558054.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2745..3431
                     /locus_tag="WJK73_001592"
     CDS             2745..3431
                     /locus_tag="WJK73_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7558055.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3563..3901
                     /gene="erpA"
                     /locus_tag="WJK73_001593"
     CDS             3563..3901
                     /gene="erpA"
                     /locus_tag="WJK73_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ7558056.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            4061..4525
                     /locus_tag="WJK73_001594"
     CDS             4061..4525
                     /locus_tag="WJK73_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ7558057.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4560..6071
                     /gene="ubiB"
                     /locus_tag="WJK73_001595"
     CDS             4560..6071
                     /gene="ubiB"
                     /locus_tag="WJK73_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ7558058.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFGKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1374 a         1358 c         1637 g         1748 t
ORIGIN      
        1 aaaatttcca gagaaccgat acaacagttg gaacttgggt ttgggaatat tacggtagat
       61 gaacttggaa cctctgttat gctatggtct tttatctcaa ttgaaaaaag cgcgaatcaa
      121 acggttcgcg cttttttcag acggcatcaa ttattttttg tcgtctttta cttcttcaaa
      181 gtcggcatct acgacatcat cgtctttctt tgcagaagca ttggcttgtt cgctttcgcc
      241 tgcttgggct tcagcttgtg cttgagcgta aaccatttcg cccagttttt ggctggctgt
      301 gcccagcgcc tcggctttgg catcgatggc ggctttgtcg tcgcctttca cggcttcttc
      361 ggcttctttc agcgcggctt cgattttttc tttttcggca gcgtcgagtt tgtcgccgta
      421 gtccgccaaa gattttttca cggagtgaat cagggcttcg gcttggttgc gggaagcgac
      481 caattcagtc agctttttat cttcctcagc attggcttcg gcatctttca ccatgcgttc
      541 gatttcttct tcgctcaaac ctgaagaacc ttggatggta atgttggctg ctttaccggt
      601 gcctttgtct ttggcggaaa cgtgcaggat gccgttggcg tcgatgtcga aggttacttc
      661 gatttgcggc ataccgcgcg gtgcaggtgc gatgtcgccc aagttgaact gacccaaaga
      721 tttgttggca gaagcgcgtt cgcgttcgcc ttgcagtacg tggatggtta ctgcgctttg
      781 gttgtcttcg gcggtagaga acacttgcga cgctttagtc gggatagttg tgttcttttg
      841 aatcagcttg gtcatcacgc cgcccatggt ttcgataccc aaggacagag gggttacgtc
      901 cagcagcaat acgtcgctgc ggccgccgct taacacttcg ccttgaatcg ctgcgcctac
      961 ggcgacagct tcgtcagggt tcacgtcttt gcgcggctct ttgccgaaga agtctttaac
     1021 ggcttcttgt actttcggca tacggctttg accaccgacc aaaatcacgt cgtcgatgtc
     1081 gccagtgctc agaccggcat ctttcaatgc ggtgcggcaa ggctcgatag agcgggcaat
     1141 caggtcttca accaggcttt cgaatttggc gcgggtaatt ttcatcgcca agtgtttcgg
     1201 gccggttgcg tccatggtga tgtacggcag gttaatttcg gtttgctggc cgctggacaa
     1261 ttcgattttg gctttttcgg cagcttcttt caggcgttgt agagccatca cgtcttgttt
     1321 caaatcaatg ccttgttctt ttttgaactc ggcgatgatg tggtcgatga ggcgttggtc
     1381 gaagtcttca ccgcccaaga aggtatcgcc gttggttgcc aatacttcga attgtttgtc
     1441 gccgtcgagg ttggcgattt cgatgatgga aatatcaaaa gtaccgccgc ccaagtcata
     1501 tacggctact ttgcggtctt tgttgtcgcc tttgtccata ccgaatgcca aagcggctgc
     1561 ggtcggctcg ttgatgatgc gttttacgtc caaaccggcg atacggcctg cgtctttggt
     1621 ggcttgacgt tggctgtcgt tgaagtaggc cggaacggta atcacggctt cggttacttt
     1681 ttcgcccaag taagcttcgg cggcttcttt cattttacgc aggacttctg cggaaatttg
     1741 aggaggagac agctctttgc cttgcgcttt cacccatgcg tcgccgttgt tggctttgat
     1801 gatttcaaaa ggcatagatt cgatgtcgcg ttggacttct ttgtcttcaa atttgtggcc
     1861 gatcaaacgt ttggcggcgt aaatagtgtt tttagcgttg gttacggctt ggcgtttggc
     1921 aggcgcgccg acgaggattt cgccgccgtc caaataagcg ataacggacg gcgtggtgcg
     1981 tgcgccttct gcgttttcga tcactttggt ttgaccgttt tcggaaatgg ccaaacaaga
     2041 gttggttgta cctaagtcga taccgattac ttttgccatg tggataatcc tatttgattt
     2101 tgcttatttt gagaaatatg ttggaacatt ttgtcccgat gggctgtaaa tagggcgggc
     2161 ggcgggctgt ttcaagctac agcatggcta taagtatata actttatgaa tatattggtt
     2221 ttatatttga tttaatacat ttggctccaa tgcattcaag cataatgttt caaatggcag
     2281 gcaggtttat tcatagacga tgccggcgag catttcctgt tcgttcaagt tgccgtactc
     2341 tttttcccag tcgtgtgaag actcgatgat gtcgcattct ttggaaaggg agacttgttc
     2401 tgcatccata tctttggcgt tcagtatgtt gaatttttcg cacagggatg cggataaagt
     2461 gatgtcaggc tgtttggctt cagaacggtt ttcttggaag gcaaagcaga atgcggtaaa
     2521 tgccgcagca tagataagat atttgccggt tttcttcatt ttctatcctt tttctgtcaa
     2581 cttaggatta aacctatgga aaatcttaaa aattatgtat tgagtaagaa aaatcataat
     2641 ttaaatttag tttatcatag ttgttccgtt tttgggatag ctaaggtaaa atatatttca
     2701 tgtttacttt agatgattga atgaagggga gcggaaggat atttatggaa acctttaaag
     2761 acagactggt ttttttatgg aaaagcgaag cgaagcaggc aaaaatcgca tccgatattg
     2821 aaatgacgat tgcgggcttc agcaggatat ggaatgaagg cggtctgccg aagtctgaaa
     2881 cattaaaaaa aatcaagcag ttgaaggggt gtagtatcga ttggctgctg accggggagg
     2941 gtaatccgtt tccggatgaa gccccaaaaa aatcccttgc ttacgatact ttgggcaatg
     3001 aagtcgatac ggacgagttt gtcttcgtgc cgagatatga tattcgggcg gctgcgggat
     3061 acgggcagtt tgtcgatcat gaggaaccgg tatttacaat ggcgttcaga cggcattgga
     3121 ttgagaatta tgttacccgc gatacgaaaa acctgtctgt aatttccgtc aagggggatt
     3181 cgatggaggg ggttttgaat gacggcgatt cgattttggt caatcatggt gaaaatacgc
     3241 cgagggacgg tctgtatgtg ttgcggatta atgaaaatct gctggttaaa cgtttacaga
     3301 ttgtaccggg cgggattatc aatgtgattt ctgcaaacga ggcttatcct gcttttgaaa
     3361 tcaatttgaa cgatttgacc gatgatgtgg agattatcgg gcgtgtcgag tggttcggca
     3421 ggacgatttg agtttggggc ttgaaattgc aggcggtcaa acttatctat tggaacaatt
     3481 cctttttcaa aggcgaagcc tgcttgcctt tgaagggggt ttgagagaga atgcagaaaa
     3541 tattatatta aggaataaca ccatgtcgga tgaaagccct attattttta ctgacagctg
     3601 ctgtgccaaa gttgccgatt tgattgccga agaaaacaat cccgatttga aattgcgggt
     3661 ttttgtcaat ggcggcggct gttcgggttt ccagtacgga tttacttttg acgaaatcaa
     3721 aaacgacgac gattttgaaa ttgagaaaaa cggtttggtc tttttggtcg atccgatgag
     3781 ctatcaatat ctggtcggtg cggaaatcga ctatacggaa agtttgcagg gttcgcaatt
     3841 cgtcatccgc aatccgaatg cggaaacaac ctgcggttgc ggatcgtcgt tttccgtatg
     3901 accgcttggt ttgtgtgatg ccgtctgaac gttcagacgg catttttact tttagaaaat
     3961 atattatcgg gatgaattca catataatcc gattgtttga agatgaatcg ggtttcccga
     4021 aaggaacggg cggaacggta tcaggcgtat ttgttccctt atgattgaga tgagtaaaga
     4081 ttaccgaaac gatttgtacg atgtatatgt ttcttacccg ccccaagtgg atcgcgggct
     4141 tatccgggag tgccttaagg agaatctcgg cgaggaaaag gcggaaggat tgatcgaatc
     4201 gctcgattcc aaacctcaag tgctggttga ggaaaaatgc acttgggcga aacgggaaga
     4261 gttgcatgat tatttcagct atttgggttt ggatattatt acccggagat atatggagtt
     4321 agaaacggtc gtgccgccgg aggaaggtga gggcgaagga gagggcgcgg atggggaaat
     4381 gcccgaatat cttgaacttc atggcgggcg ggaagatgat atttccgcac cttcgcaacc
     4441 cgaaccgccg acccgcaata tcaaaccgct gcttttcggg ctgctgattg cctttttggg
     4501 ctatctgctc ggtaagattt tttgattgtc cgataaatgc tgtattcggg attttatata
     4561 tgaaatggtt gaaacgcctg acggtcattg tcgggacttt ttaccgctat cggctggcag
     4621 gtctgtgtgc ttcgctgatg ggtagcggtt ggatatgcgc tctgctgaaa atgatgccgc
     4681 agtcgtccaa attgaaaaac gaaccgcctg ccgtccgtct gcgccttgcc ttggaaagcc
     4741 tggggccgat tttcatcaag ttcgggcagg ttttgtccac acgccccgat ttgattccgc
     4801 acgattacgc cgtcgaactg gcaaagctgc aagacaaagt gccgcctttt gacgcgcggc
     4861 tttcgcgtga acaaatcgaa aaatcgttgg gtcagtccat cgaaaagctg tatgcggaat
     4921 ttgaaaccga gcccatcgcc agcgcgtcca tcgcccaagt acacaaagcc cgcctgcatt
     4981 cgggcgaacg agtggcggtt aaagttttgc gccccaacct tttgcccctt atcgaacagg
     5041 atttgtcgct gatgcgtttc ggcgcggcct gggtcgaacg cttgttttca gacggcaggc
     5101 gtctgaagcc gctcgaagtg gtggcggagt tcggcaaata cctgcacgac gagttggatt
     5161 tgatgcgcga agccgccaat gccggacagc tcgggcgcaa tttccacaac agcaatatgc
     5221 tgattgtgcc caaggtgttt tacgactact gcaccagcga cgtgctgacg attgaatgga
     5281 tggacggtac gccggttgcc gacatcgcca aactcaaagc agacggcatc gatttgcaca
     5341 aactcgccga ttacggcgtg gaaatctttt tcacgcaggt cttccgcgac ggctttttcc
     5401 acgcggatat gcaccccggc aatattttgg ttgccgccga caaccgctac atcgccctcg
     5461 atttcggcat cgtcggcacg ctgaccgatt acgacaaacg ttatctcgcc atcaatttcc
     5521 tcgccttttt caaccgcgat taccggcgcg tcgccaccgc ccacatcgaa tcgggctggg
     5581 tgcctgccga cacgcgtgcg gaagaattgg aagccgccgt ccgcgccgtg tgcgaaccgg
     5641 tgttcaacaa accgatttcg cagatttcct tcggcttggt gctgatgcgc ctgtttgaag
     5701 tcagccgccg cttcaatgtc gaaatccaac cgcagcttgt gctgctgcaa aaaacgctgc
     5761 tcaacatcga aggcttgggc cgccagctcg atcccgattt ggacttgtgg aaaaccgcca
     5821 aaccgttttt ggtgaaatgg atgaacgggc aggtcggccc taaagccctt tggcgcaacc
     5881 tcaaaaacga agcccccgac tgggcgcaaa tcattccttc attgccgcgc aaaatcagtg
     5941 cgttgattga tgaaaaccgc cagcaggaaa tgcgtgatgc ctatatccat ttggtcaaag
     6001 tgcagcagcg gcaaagcctg tggctggctg tgattgcggt tgttttgctg ctgattttgc
     6061 ttttgaaata ggctttgtcc gaatcatcgc ccgactccgc ccgtttataa ggaaatc
//
LOCUS       DAWKOD010000118         6108 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000118 DAWKOD010000000
VERSION     DAWKOD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.118"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..403
                     /locus_tag="WJK73_001596"
     CDS             20..403
                     /locus_tag="WJK73_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ7558059.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            518..991
                     /locus_tag="WJK73_001597"
     CDS             518..991
                     /locus_tag="WJK73_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ7558060.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMRIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1117..2220
                     /gene="mnmA"
                     /locus_tag="WJK73_001598"
     CDS             1117..2220
                     /gene="mnmA"
                     /locus_tag="WJK73_001598"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ7558061.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2291..3961
                     /locus_tag="WJK73_001599"
     CDS             2291..3961
                     /locus_tag="WJK73_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ7558062.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4073..5707
                     /locus_tag="WJK73_001600"
     CDS             4073..5707
                     /locus_tag="WJK73_001600"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ7558063.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5722..5924
                     /locus_tag="WJK73_001601"
                     /pseudo
     CDS             5722..5924
                     /locus_tag="WJK73_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5935..6108
                     /locus_tag="WJK73_001602"
                     /pseudo
     CDS             5935..6108
                     /locus_tag="WJK73_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1599 a         1647 c         1536 g         1326 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ttcttacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac tcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatccgc tataagatta
      481 agcacattcc ccgtctgata ttataaggag cagaaagatg aaaaaattat tggcggccgt
      541 gatgatggca ggtttggcag gcgcggtttc cgccgccgga gtccacgttg aggacggctg
      601 ggcgcgcacc accgtcgaag gtatgagaat aggcggcgcg ttcatgaaaa tccacaacga
      661 cgaagccaaa caagactttt tgctcggcgg aagcagcccc gttgccgacc gtgtcgaagt
      721 gcatacccac atcaacgaca acggcgtgat gcggatgcgc gaagtcgaag gcggtgtgcc
      781 tttggaggcg aaatccgtta ccgaactcaa gcccggcagc tatcacgtga tgtttatggg
      841 tttgaaaaaa caattaaaag agggcgacaa gattcccgtt accctgaaat ttaaaaacgc
      901 caaagcgcaa accgtccaac tggaagtcaa aatcgcgccg atgccggcaa tgaaccacgg
      961 tcatcaccac ggcgaagcgc atcagcacta atctgctgaa aatgtttgaa atgccgtctg
     1021 aaaaagcccg ggctttcaga cggcattttt atgcccgcct ttaaaatgtg ttaaaatccg
     1081 cctttaaaaa ccgccgtttc caaccatatt ctacgcatga atacgacagc aaacaccacc
     1141 aatatcatcg tcgggctctc cggcggtgtc gattcttccg taaccgccgc cctgctcaag
     1201 cagcagggtt atcaagtgcg cggtgtgttc atgcagaact gggaagacga cgacaacgac
     1261 gaatattgca gcatcaaaca ggattcgttc gatgccatcg ccgttgccga tattatcggc
     1321 atcgatatcg acatcgttaa tttcgccgcg caatataaag acaaagtctt tgcttatttt
     1381 cttcaggaat acagtgcggg gcgcacgccg aatccggatg tgttgtgcaa cgccgaaatc
     1441 aaattcaaat gctttttgga ctacgccgta gggcagggcg cggataccat cgccaccgga
     1501 cactatgcgc gcaaagaagt ccgcaacggc gtgcattacc tgctcaaagg tttggatcga
     1561 aacaaagacc aaagctattt cctctaccgc ctcaagcctt tccaactcga gcgcgcgatt
     1621 tttccgttgg gcggtttgga aaaacccgaa gtgcgccgcc ttgccgccga atttaatttg
     1681 ccgactgccg ctaaaaaaga cagcacgggc atctgtttca tcggcgaacg cccgtttcgc
     1741 gagtttctgc agaaatacct gccgaccgac aatggtaaaa tggttacgcc cgaagggaaa
     1801 accgtcggcg aacacgtcgg gctgatgttc tacacgctcg gacagcgcaa aggtttgggc
     1861 attggcggcg cgggcgaacc gtggtttgtt gcggctaaag atttgacgaa aaacgaactg
     1921 attgtcgtgc aaggacacga ccatccgctg ctttataccc gcagccttgt gatgaacgat
     1981 ttgagtttca cgctgcccga acgcccgaag gcaggacgct atacctgcaa aacgcgttac
     2041 cgtatggcgg acgcgccttg tgagttgcgc tatttggatg atgaaaccgc cgagctggtg
     2101 tttgacgaac cgcaatgggc ggttacgccg ggtcagtccg ccgtgctgta cgacggcgat
     2161 atctgtttgg gcggcggtat catccaaacg accgacaaac ccgtcatcat cacacgataa
     2221 aggtaatgcc gtctgaaacg gttttcagac ggcattgttc cgctcaattc cactttaaag
     2281 accgatacct atgaaaaaaa tttggttaga cagctacgag aagggcgtca gtgccgaaat
     2341 cgacatcacg caatacaatt ccgtcagcga cgtattccgc caaagcgtgg aaaaatttgc
     2401 cgaccaacct gcttttcaaa acatgggtaa gacgctgact tatgccgaaa ccggcaaact
     2461 ggcaactgat ttcgcctctt atctgcaaaa cgtcctcaag ctgccgcgcg gcgaacgtgt
     2521 tgccattatg ctgcccaacg tattgcaata cccgattgcc cttttcggta ttttgcaggc
     2581 aggtttggtg gcggtgaaca ccaatccgct ctatacaccg cgcgagttgg agcatcagtt
     2641 gaaagacagc ggtgcgaccg ccatcatcgt tttggaaaat ttcgccaaca cgctggagct
     2701 ggtgctgccg cgcacgcaga tcaaacacgt catcgtcgcc tccgtcggcg aaatgttcgg
     2761 gctgcttaaa ggttcgctga tcaatttcat catccggaaa atcaagaaaa tggtgcccga
     2821 ataccgtatt cgggaaaccg tttcctttca gacggcattg aaagaagggg cgaagcacgt
     2881 tttccaacct gtcgcattaa accgcgaaga taccgcgctg ttgcaataca cgggcggcac
     2941 gacaggtgtt gccaaagggg cggtgctgag tcacggcaac atctgcgcca atatgcttca
     3001 ggcaaaagaa tggattaaaa accagttgcg cgagggcaaa gaaaccgtta tcgccgcctt
     3061 gccgctgtac catattttcg ccttaaccgt gaatctgatg atttttgcca atgccggctc
     3121 gaaaatcatc ctgattacca acccgcgcga tatgaaaggc tttatcggac aactgaaaaa
     3181 acagcgggtt aatgtattta tcggcgtgaa cacgctgttt aacgcgatgg ttaaccgtcc
     3241 tgattttgcc gaagtcgatt tttcaggatt gcggctgact ttgggcggcg gtatggcgac
     3301 ccaaaaagcc gttgccgaaa aatggaaaaa aatcaccgac acgcccatcg tcgaagccta
     3361 cggtctgacc gaagccagcc ccggcgtgtg ctgcaacccc ttaaacatcg aatcatacag
     3421 cggcagcatc ggtttgcccg tctcgtccac cgaagtcgaa ctgcgcgacg caaacggcaa
     3481 agaagtcccc gtcgggcagc cgggcgaatt gtgggtaaaa ggccctcaag tgatgcaagg
     3541 ctactggaac cgtcccgaag aaaccgctaa agccatagac gcgcgcggtt tcttggaaac
     3601 cggcgatatt gccgtcatgg acgaaaaagg ctggttgaag ctggttgacc gtaagaaaga
     3661 cctcattgtt gtttccggat tcaatgttta tccgaacgaa atcgaagaag tcatcgcgca
     3721 tcacggcaaa gtgatggaag ttgcctgtat cggcgttccc gacgaaaaaa ccggcgaggc
     3781 actcaaagta ttcgtcgtca gaaaagaccc gtctttgacg aaagaagaac ttatcgcctt
     3841 ttgccgcacc gaattaaccg catataaagt accgaaagat atcgaattcc gcgacgagtt
     3901 gcccaagtcc aacgtcggca aaatcctgcg ccgcgagttg cggcaaagta ccgggaaata
     3961 aagaaaagat gccgtctgaa aacagccgtc caccgttcag acggcatccg tccgtttgcc
     4021 agaaccgcgc gcttcacgtt aaaatcacgc attccaatat gggtattcca tcatgaccaa
     4081 attcattttc gtaaccggcg gcgttgtctc ctcactgggt aaaggtatcg ccgccgcttc
     4141 tattgccgcc atcctcgaat cgcgcggctt gaacgttacc atgctcaagc tcgatcctta
     4201 tatcaacgtc gatcccggca cgatgagccc gttccaacac ggcgaagtgt tcgtaaccga
     4261 cgacggcgcg gaaaccgacc tcgacttggg acactacgaa cgctttatcg attccacgat
     4321 gacccgccgc aacagcttca gcacggggca ggtgtacgaa aacgtcatcg ccaaagaacg
     4381 acggggcgac tatttgggcg gcacggttca agtcattccg cacattaccg acgaaatcaa
     4441 acgccgcatc cacgaaggcg cggcaggtta cgatgtggcg attgtcgaaa tcggcggtac
     4501 agtcggcgat atcgaatctt tgccattctt ggaagccatc cgccaaatgc gcagccagct
     4561 cggccgcaac aatacattgt ttgcccacct gagctatgtt ccttacattg ccgccgccgg
     4621 tgaaatcaaa accaaaccga cccagcatac cgttaaggaa atgttgagca ttggtttgca
     4681 accggacatc ctgatctgcc gtatggacag agaaatgcct gcggacgagc gccgcaaaat
     4741 cgccttgttc tgcaacgtgg aagaacgcgc gattgtgggc agctacgacg tggacagcat
     4801 ctacgaatgc cccgaaatgc tgcacgacca aggcatagac aacatcatta ccgaacagtt
     4861 gcagcttaac gtacaacagg cggatttgac tgcgtggaaa aaaatcgtcc acgccgtcaa
     4921 aaatccgaaa cacaccgtca aaatcgcgat ggtcggcaaa tacgtcgatt tgaccgagtc
     4981 ctacaaatca ttgattgaag ccttgaaaca cgcgggtatc cacaccgaaa ccgacgtgca
     5041 aattaccttt gtcgacagcg aaagcatcga gaaaaacaaa ggcgacgttt ccatgctcaa
     5101 agatatggat gccatcctcg tccccggcgg tttcggttcg cgcggcgtgg aaggcaaaat
     5161 cgccgccgtg cgctacgccc gcgaaaacaa cgtgccatac ttgggcatct gcctcggtat
     5221 gcagattgcg ctgatcgaat acgcccgcga cgtggcaggt ttgaaaggtg cgaattccac
     5281 cgagttcgac ctcaaatgcg ccgcccccgt cgttgccctg attgacgaat ggcaaactgc
     5341 cgacggcagc gtcgaaaccc gagacgagtc cgccgatttg ggcggcacga tgcgtttggg
     5401 cgcgcaagaa gtcgaattga aagcaggcag cctcgccgcc aaaatctacg gcagcggaca
     5461 tatccgtgaa cgccaccgcc accgctacga agtcaacaac aactatgttc ctcagttgga
     5521 aaaagcaggc ttggttatcg gcggcgtatc cgccggacgc gaacgcttgg tcgaaaccat
     5581 cgaactgccg aaccatcctt ggttcttcgc ctgccagttc caccccgagt ttacctccaa
     5641 tccgcgcaaa gggcatcctt tgttcaccgc gtttgtcaaa gctgcgttga acaataaaaa
     5701 agcctgataa agcgttactt gatgatcaaa atgccgtctg aaagcctgaa tgaagacttt
     5761 cagacggcat tttcgcaaat cggggattat aaaagccatc aatttaattt ttatggtttt
     5821 atctaatatt taccaactga atattgttaa gcggggcgtg aaactcaagt tgagaccttt
     5881 gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggagatt tctcatgagc
     5941 accttctttc agcaaaccgc acaagccatg attgccaaac acatcgaccg cttcccacta
     6001 ttgaagttgg accaggtgat tgattggcag ccgatcgaac agtacctgaa tcgtcaaaga
     6061 acccatttac cttagagacc accgcggccg tcccgcctat ccgctgct
//
LOCUS       DAWKOD010000119         6036 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000119 DAWKOD010000000
VERSION     DAWKOD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.119"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(223..1560)
                     /gene="ribB"
                     /locus_tag="WJK73_001603"
     CDS             complement(223..1560)
                     /gene="ribB"
                     /locus_tag="WJK73_001603"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ7558064.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMAGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1776..2786)
                     /locus_tag="WJK73_001604"
     CDS             complement(1776..2786)
                     /locus_tag="WJK73_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7558065.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDAYWADTNRSKNA
                     GWILFDLQQLADKKVDLILHPSSLNYRDIPKGADFSDNDFVRHFETLVEGRYYIANAW
                     TKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHN
                     IKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIIL
                     IYRKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFF
                     SE"
     gene            complement(2942..3535)
                     /gene="ribA"
                     /locus_tag="WJK73_001605"
     CDS             complement(2942..3535)
                     /gene="ribA"
                     /locus_tag="WJK73_001605"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ7558066.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANRQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3528..4202)
                     /locus_tag="WJK73_001606"
     CDS             complement(3528..4202)
                     /locus_tag="WJK73_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558067.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGITAA
                     EGDENV"
     gene            4356..4440
                     /locus_tag="WJK73_001607"
     tRNA            4356..4440
                     /locus_tag="WJK73_001607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4390..4392,aa:Leu,seq:tag)
     gene            4961..5995
                     /gene="purM"
                     /locus_tag="WJK73_001608"
     CDS             4961..5995
                     /gene="purM"
                     /locus_tag="WJK73_001608"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ7558068.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1460 a         1491 c         1590 g         1495 t
ORIGIN      
        1 gccgttcttt ccctgaaaga gagaatccaa aaaaccaaag ccacaggaat ttatcggaaa
       61 tgacaaaaac ccgacgaacc ggattcccgc ctgcgcggga atgacgaatt agaagttacc
      121 cgaaatttgc aaaaacaaaa tccaacccaa cagaccgggt tttcgtttgc acggaaataa
      181 tgcaataaat aaagcaaata taaagtattt gaatttacta tattattttt ccgcttcttc
      241 aaagccgacg acttccaaac cgaagccggt caggccggtg aaagatgagg gctgcccgag
      301 gacgcgcagt tttttgacgt tgaggccgac gaggatttgt gcgccgatgc cgtagctttt
      361 gctgtcccat ttgtaggctt ggtttgcacc tttgggcagg gtgcggtcga gcagggatgc
      421 gccgtcttcg gcgcggtgta agaggatgac gacgccgctt tcggcttgtt ggatgcgctc
      481 aagggctttg ggcagcgacc atgaatggcg cggattggct tggatgaagt ccatcacgct
      541 gaagggttcg tggacgcgga cgagggtttc ggtgtcggcg gcgggcgtgc ctttgacgag
      601 ggcgaggtgg gtttcgccgg agagtttgtc gacgtaaacg tgttgttgga actcgcccca
      661 cggggtttgt acaggcgcat tgcccatgtc ttcaagcagg ctttcggtac ggctgcggta
      721 ttcgatgagg tcggcaatcg tgccgatttt gagcttgtgt tcttcggcga atttcatcag
      781 ttcgggcata cgcgccatcg tgccgtcgtc gttgatgatt tcgcagatga cggcggcggg
      841 aatcaatccg ttcatttgcg ctaaatcgac accggcttcg gtgtgtccgg cgcggacgag
      901 tacgccgcct ttttgcgcgc gaagcgggaa aatatggccg ggttggacga tgtcttcggg
      961 tttggcggtc ggggaaacgg cggtttgaat ggtcagggcg cggtcggcgg cagaaatgcc
     1021 ggtggtaatg ccgtgtgcgg cttcgatgga gacagtaaag ttggtgccgt attgcgcgcc
     1081 gtttttctgg gtcatcatcg gcagtccgag tttttcgacc atttcgcccg ccatcggcag
     1141 gcagaccagg ccgcgcgcgt gtttaatcat aaagttgacg gcttcgggcg tgacgaattg
     1201 cgccgccatc agcaggtcgc cttcgttttc gcggtcttcg gcatcggtga tgatgaccat
     1261 tttgccggct ttgatgtcgg ccaggatttc ggggatgggg gagatgtggg acatggtgtg
     1321 ttttcctgtg tgttcgggcg ggcgggcggt gtgttcggta tcgagccaaa aggcgggcat
     1381 ttttgccgcc tgttcgattt tacgggcttt tttctcgccg aaggatttgt ttttcaaaag
     1441 ggcggagagt tcgcctgtgt tgagggcaac ggcttcagca aaacgagttt gcaaaccgcc
     1501 gtagtatttt tctatccact gccgcaggtt gtggcggcgc agaccggctg tgtcggtcat
     1561 cgtttcatcc tttgtagaaa tcggatgtag cggattgtaa tcgcgtattt accaaaaagc
     1621 aaatctgttt ttttcgccaa accgaattat ttgctttttg gtaaacagat gccgtctgaa
     1681 gccggattcg tggcttcaga cggcattgcc gcaatccccg ccggcggggc ggctgtttct
     1741 tcccgcccgc ttccgattcg gggtttgccg gaagcttatt ccgaaaaaaa tttttgataa
     1801 acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat cggcaatcct
     1861 atgatgaatg cggtcttccc gataaaaacc ggacggattg ccttcgcgcc gaatatcttt
     1921 ctgcattttt ctttaaatga aaaatatttc cggtagatga ggataatgtt tttggaaaaa
     1981 taaagcctta ccaatataga cctcagatag ccgatattgt cgaagacaaa tttttgcaaa
     2041 ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacaccccg gtcgagatgc
     2101 gtcagcacat cgctgaaatt tttgtatttg atgttgtggc tgatagagcc gccgagaacg
     2161 cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg cgccaattgc
     2221 aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaaacagatt gtttttgatg
     2281 attatttccc gcctgacaat ctttgtccac gcgttggcga tatagtaccg cccctccacc
     2341 agcgtttcaa aatggcggac aaaatcatta tccgaaaagt ccgccccttt ggggatgtcg
     2401 cggtaattga gggacgaggg atgcaggatc aaatcaacct ttttgtctgc aagttgttgt
     2461 aaatcaaaga gaatccaccc cgcgtttttt gaacggttgg tatcggccca ataggcgtcg
     2521 ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt gcgggcatcc
     2581 gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc tgcatattcg
     2641 tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat ttcataatcg
     2701 gcaaaatttt cggcaagcac ggaatccacg cagcagcgga gatatttttc cacattgtaa
     2761 atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga tgtttgcgcg
     2821 tatgcctcaa acccgcaccc gcaatgcgtt tgcatccact ataaaactta ttgctttatc
     2881 aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct gcaactttgc
     2941 cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag cgtttgtttt
     3001 caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc gcatctttca
     3061 gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg cccagatatt
     3121 cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg agtgccaaat
     3181 tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt ttgttaatca
     3241 gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc tctgtctgta
     3301 ccgccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag aacagcgcat
     3361 cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct gaaaaattac
     3421 cgacggtcag cgcgacgtgt tcctgcctgt ttgcctcttc aaagccgtgc atcgtaaata
     3481 cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca gacattttca
     3541 tctccttctg cggcagtgat gcccaacagc agtttcaaag gttcggacaa agccaaggca
     3601 agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc aaattccaca
     3661 tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat ttgcgccgcc
     3721 gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg tacatccgag
     3781 gcaacattca tccagccgct ttgcgcgcaa cggcaggcaa gtgaaacatc ggcaacattt
     3841 tcatctagat tccatatgtt ttccaaacct tctccctgtt gggacaggca aaccagccga
     3901 aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac gcctttacag
     3961 cgcgaaaata ccgaatccag agccatgaaa agccgtttca gaccttcctc attcccgctg
     4021 ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa aaccgaacgg
     4081 tctatcgaag ccgtccccat atccattacc gtctgcgccg tcagatacgc cgcccgaacc
     4141 tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa agcagcaggc
     4201 atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca ggcggacgaa
     4261 aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa accttgcaca
     4321 aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga cgtggcgaaa ttggtagacg
     4381 caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc gtccgcacca
     4441 ataatgataa ataaattata tagttaatgc ttatcgggac aaattagtgc cacaaaacat
     4501 aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca acacgcagaa
     4561 cggtacataa agtgccgcca tttgttccgc accgaatcgg aaacgggtgc ttccgcccgc
     4621 aatcagattc actgccccta aatgccgggg tttcaaccgg aaagaagaaa atattgattt
     4681 tgatgatggc atcaaaaaac cccttccgcc cgtctgccaa aaggaatggt taagaaaagc
     4741 aatgcgtctt ttgatggaac gcgatatata aggaattgag gggaagggca ggcgggtttc
     4801 tgcccgttaa tcctgcggac ggtcccgcca aacccgacac gcgcgggcat atggcggatt
     4861 gtttacaata ttgtctaaaa atctgaacct gcaaacgtga aataaggtag aatacgcccc
     4921 gttattttac cgtccaatct ccatttacca aggaaaaacg atgagtactt cattgagtta
     4981 ccgcgatgca ggtgtcgata tcgacgcagg cgaccaactg gtcgaaaaca tcaaaccgtt
     5041 tgccaaacgc acgatgcgcc cggaagtatt gggggatttg ggcggcttcg gcgcgttggt
     5101 cgaaatcggc aagaaatata aaaatcccgt attggtcagc ggtacggacg gcgtgggtac
     5161 caagctcaaa cttgcctttg attgggataa acacgatacg gtgggcatcg accttgttgc
     5221 aatgagtgtc aacgatattt tggttcaagg agcagaacct ctgtttttct tggattattt
     5281 cgcctgcggc aaattggatg ttccgcgcgc gaccgatgtg atcaaaggca ttgcacaagg
     5341 ttgcgaagaa tccggctgcg ccctgattgg cggggaaact gccgaaatgc cgggtatgta
     5401 tcccgtcgga gaatacgatt tggcgggttt tgctgtcggc gtggtggaaa aagagaatgt
     5461 cattaccggc cgcagcatcg gcgcgggcga tgtggtattg ggtttggctt ccaacggcgc
     5521 acattcaaac ggctattccc ttatccgtaa aatcatcgaa cgcgacaatc ccgatttgga
     5581 tgccgagttt gataatggca aaaccttgcg tgaggctgtt attgcgccga cccgtctgta
     5641 tgtgaaaccg attcttgccg ctttggaaaa atttaccatt aaaggtatgg cacacattac
     5701 cggcggcggc attaccgaaa acgtgccgcg cgtgttgcct gaaaacacgg ttgcacaaat
     5761 cgatgctaaa tcgtgggaat tgcccaagct cttccaatgg cttcaaaagg cgggcaatgt
     5821 ggaaacccaa gaaatgtacc ggacctttaa ctgcggcatc ggcatggtcg ttattgttgc
     5881 tgccgaagat gccgatgcgg ttcagggtct cttgggtgaa caaggtgaaa cagtttaccg
     5941 tttaggtttg atccgtgagc gtcagggaga cgagcatcaa acccaggttg cctgattgct
     6001 tctatagtga aatgccgtct caaacatggg tttgga
//
LOCUS       DAWKOD010000120         6023 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000120 DAWKOD010000000
VERSION     DAWKOD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.120"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJK73_001609"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJK73_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ7558069.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJK73_001610"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJK73_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ7558070.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJK73_001611"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJK73_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ7558071.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAEQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQADVQTASEAKTASEAE"
BASE COUNT         1351 a         1699 c         1624 g         1349 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgtt tgtaacgcag accgaccaaa
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccaac
      301 tgctcgcgcg cggtcaatgc gtttgccacg tcttgaaatg cggattggac ggcggcttca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat cgaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatggaa ggtgcgaaca accaaacgcc ggtgccgctt
      481 ttgaacagcc cgcccaattc ggcagaatgc gtatcgacgc ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcataa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcgtgcc aacccaaatc gacggcgcgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcagcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa caccgacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcaaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaattcgata
     1861 atcaaaatcg catttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacgga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgtcagc ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacgatg aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag acggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt gatgccggca gatttgatcg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgttc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaacgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgcc ggtgcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAWKOD010000121         5967 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000121 DAWKOD010000000
VERSION     DAWKOD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.121"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            11..187
                     /locus_tag="WJK73_001612"
     CDS             11..187
                     /locus_tag="WJK73_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558072.1"
                     /translation="MAHQAPHCVLPERDYPPIRFYRYKQTDFNRKGMGIKSVSDTSRA
                     MPSENQSPLSDGIV"
     gene            264..1211
                     /locus_tag="WJK73_001613"
                     /pseudo
     CDS             264..1211
                     /locus_tag="WJK73_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(1364..2488)
                     /gene="rluD"
                     /locus_tag="WJK73_001614"
     CDS             complement(1364..2488)
                     /gene="rluD"
                     /locus_tag="WJK73_001614"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ7558073.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2487..3290
                     /locus_tag="WJK73_001615"
     CDS             2487..3290
                     /locus_tag="WJK73_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ7558074.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3348..5567)
                     /locus_tag="WJK73_001616"
     CDS             complement(3348..5567)
                     /locus_tag="WJK73_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ7558075.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1465 a         1884 c         1438 g         1180 t
ORIGIN      
        1 tcaacagggg atggcacatc aagcaccgca ctgcgtgttg cccgaacgag actaccctcc
       61 gattagattc tatcgctata aacagacgga tttcaaccgg aaaggaatgg ggataaagtc
      121 cgtttccgac acctctcggg cgatgccgtc tgaaaaccaa tctccacttt cagacggcat
      181 tgtttaggca cggtcatttt gcaaattaag gttaaggcgt gtatgcttgc cttcccttaa
      241 aaacaaacac gcaacgcatt attatgaata tcctcagtaa aatcagcagc tttatcggaa
      301 aaacattttc cctctgggcc gcgctctttg ccgccatcgc ttttttcgcg cccgacacct
      361 tcaaatgggc ggggccttat attccttggc tgttgggcat tattatgttc ggtatgggtt
      421 tgacgctcaa accttccgac ttcgatattt tgttcgaaca tcccaaagtc gtcatcatcg
      481 gcgtaatcgc acaattcgcc attatgccgg caaccgccta gctgctgtcc aaactgttga
      541 acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg ctgctgcccg ggcggtacgg
      601 cttccaatgt gatgacctat ctggcgcgcg gcaatgtggc tttgtcggtt gccgttacgt
      661 ctgtttccac cctgatttcc ccattgctga ctcccgccat cttcctgatg cttgccggtg
      721 aaatgctgga aatccaagcg ggcagtatgt tgatgtccat cgtcaaaatg gttttgctcc
      781 ccattgtttt gggtttgatt gtccataagg ttttgggcag taaaaccgaa aagctgaccg
      841 atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat tatcggcgcg gttgttgggg
      901 caagcaaagg caagattatg gaaagcggcc tgctgatttt tgcggttgtc gtactccaca
      961 acggcatcgg ctacctgctc ggcttctttg ccgccaaatg gaccggcctg ccttatgatg
     1021 cacaaaaaac gctggccatc gaagtcggta tgcaaaactc tggcctggcc gccgcgcttg
     1081 ccgccgcaca ctttgccgcc gcgccggtcg ttgccgttcc gggcgcattg ttcagcgtgt
     1141 ggcacaatat ctccggctcg ctgctggcaa cttattgggc ggccaaagcc ggtaaacata
     1201 aaaaacccta aacgcctttc atcataatgc cgtctgaacg gggcaaacga aaccggcggt
     1261 ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg cggggacgat tgcccggctg
     1321 ctccggttcg gctgcctgcg ttcgccccgc cttgcaaacc gcctcactcc cttacataaa
     1381 ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc gtcgtccgcg ccgaatttgt
     1441 cctgccattc ctcttcattg ctcaaagacg aatccaaacc ggcttcaaga cggaggacag
     1501 acaacaaatg atatatgtcg tccggaatcg gtgcctcaaa cgaaacggtt tcgccgcttt
     1561 ccggatgggt gaaactcaag cggtaggcgt gcaacgcctg acgcgcgccc aaacttttaa
     1621 cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt gccgtaaacc gggtcggcgg
     1681 caagcggatg gttggcctcg cgcatatgga cgcggatttg gtgcgtcctg cccgtttcga
     1741 gcgagcattc gatatagctg tgggtaagat agcgttccaa cactttgacg tgggtaacgg
     1801 ctggtttgcc gccgaatttg acgactgcca ttttcaggcg gttgtgcgga tcgcgtccga
     1861 tttgggtttc gattttaccg tcaaagggga cgatgccgtt ggcgacggcg cggtagatgc
     1921 gtttgaccgt gcgctcttga agctgcctca cgagggaatt ttgcgccgac agggttttgg
     1981 caaccaccat cagcccgctg gtttccttgt ccaaacggtg tacgatgccc gcgcgcggta
     2041 cttggctcaa ttcgggacag tgcgccaaca ggccgttgag cagcgtcccc gtccagttgc
     2101 ccgccgccgg atgcaccacc agtccggccg gtttgttgac gacgatgacg gtatcgtctt
     2161 cgtaaacaat atccaaagcc ataggctctg gaacaaacgc cagattttcc tcactcggac
     2221 ggacggttac acaaatttgc tcgccgccta tcattttgtc tttgggttgc gaaggtttat
     2281 cgtttacaat aaccgcgcct tctttaatcc atgatgtcag gcggctgcgc gagtagtcgg
     2341 gcagaagttt cgccaacacc gcatccaacc gcccgccggc aagctcgagc ggaacggtca
     2401 gaccgatacg gttttcagtt tcggaagctg acgcaaagtc tgaatcgtcg ctataatcgg
     2461 attcattatc aaaggaagta ttctgcatga aaaaaattct tttaacggtt tcattaggtt
     2521 tggcactgag tgcctgtgcc actcaaggta cggtcgataa agatgctcag attacccaag
     2581 attggagtgt ggagaagctc tatgccgaag cccaggacga attgaacagc agcaattata
     2641 cgcgggctgt caagttatac gaaatcttgg aatcgcgctt ccccaccagc cgccatgccc
     2701 ggcaatccca actggatacc gcatacgcct attataaaga cgatgaaaaa gacaaggctc
     2761 tggcggcaat cgaacgcttc cgccgcctcc atccgcagca tccgaatatg gattacgcgc
     2821 tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc cttcttgaac aaactggcct
     2881 cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga agcgtaccag gcgtttgcgg
     2941 aactcgtcca acgcttcccg aacagcaaat acgccgccga tgcaaccgca cgcatggtca
     3001 aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc gcgttactac atgaaacgcg
     3061 gcgcatatat cgccgccgcc aaccgcgccc aaaaaattat cggcagctat caaaatacac
     3121 gctatgtcga agaatcgctc gccatattgg aacttgccta ccaaaaactc ggcaaaccgc
     3181 agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt cccgaaaagc ccgtttttga
     3241 cgcacgcctg gcagcccgac gatatgccct ggtggcgtta ctggcattaa gcccatcttc
     3301 gtaatgccgt ctgaagccgt ttcagacggc attttatgtt tcaaacctca ttcaaacggt
     3361 tttttctgcc aatagaattt ataccctctc aaacgttgag ccttcacgcc gccgcgtccc
     3421 aagccgaatt gcagtgcgcc cgacaaatcg gtacgcagca gtttgatgcc gtgtgcgcgg
     3481 acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt tggcataacc gcttgaagca
     3541 acggcatatt cgggcgagac ggcattgagg aacacgcccg acgaggacgt attgctgccg
     3601 tgatgtccca aaaccaaaac ctggctgtac aggttgcctc catacttgcc gaccaggctt
     3661 tcctcgccct tcgtatccaa atcgcccgtt accagcagtg ccgcaccgcc cgccacaaca
     3721 cgcaaaacac aacttttccc attatcatcg atgtttttgc gttcagacgg catcaaaaac
     3781 tcaaaatcca cgccgtccca ttgccaacgc tgttccgcac aatgccgcgc cccctcataa
     3841 aattccggct gtccggcata aatcccgccg ttcggtatct tgccgactgc ctgaaaaccg
     3901 ccgtcgtggt cgctgtcgtg atgcgacaga accagcttgt ccaaacggcg gacacccgcc
     3961 gcattcaaac tcggcacaat ccccatctgc gccgccgctg ccgttccggt gtcaaacaaa
     4021 agatgatgat ttgccgtctg aaccgacacc gacaaaccct gccccgcatc ccaaaccgta
     4081 accgcaacct cattttccgg cacgccgggt gaacggtaaa acacaaaccc tgccaacagc
     4141 aacaccgccc acggacgcaa acccaagccg cgcggcagca gcaacagcaa agcggcacac
     4201 accgccaaca ccaacagcgg caaaggtgcg gaggcaacgg caaactcggg cgacacatcg
     4261 gcaagccaca ccaaaaaccg caaagtatat tccgccaaaa atgcccccaa ctgttgcaaa
     4321 ggcgcaaacg gcacgaccga acccagcaac gccagcggcg tcaataccca agaaaaccac
     4381 ggaattgcca ccgcattgac caaagggctg accaaaggca gcgaagcaaa cagataaccg
     4441 agcaaaacca gcgacaacac cgaagccgcc cactgcccgc gcaaagcagt ttgccgtttt
     4501 ccctcgtgca aacgccccga gcacgcccat atcagggccg ccaccaaacc gaaagacagc
     4561 caagtcccca cacccaagac cgccgacggg tcgaacagca gcaccgccgc caacgcctgc
     4621 caccacgtcg cccacgccga caatcttccc ctgcgccaag cccacgcaaa cgccgccaac
     4681 atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa gcagcgcgta aaacagcgcg
     4741 cctgcacacc ccgccgccaa aacccacgcg cgcggccggg caggaaggcg cggggaacag
     4801 gcaagcagcc gcttcgccag ccacgcaaac attaccgcca ccatcgtaac gtgcaaaccc
     4861 gaaatactga ccaaatgcgt cagccccaac ggccggaacg cctgccacaa ttcggggcgc
     4921 aatgccgact gttcgcccac gctcaacgcg cgcatcagcc cgatgccgtc tgaaagcccg
     4981 ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc tgcgccaaac cgcaatcccc
     5041 caaccgcttc cgccatgcag caaaaccctg tccgcaccga ccgtccccgc gccgcctatt
     5101 ccgttggata atgcccacgc ctcacggttc aaaccgcgca ggttcaactc cccgacgaca
     5161 gggtgcacac gtgccgttat ccgccatctg ctcccgaccg cccattcgcg ccgtttgtag
     5221 tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca cagcctttgc cgcaaactgc
     5281 acacgccgcc cgtccgacct cggcatatcc gccacttcca ccgtcaaagg cacgcctgaa
     5341 acggcttctg cccaccattg ggaagacaat gccgcctccg tcctgaatac gccgtaagcc
     5401 gcgcccgcca acacgcacag catcagaccg gcaaacgcaa aacgccttgc aaacacagca
     5461 aaaaccgcaa aagccgccag ccaaaacagc caatgcggca cgaccggcag cgcgaacgaa
     5521 gccgccgcac cgacaaccca acacggcaaa aaccaacgac ggaacacgtc tccccacccc
     5581 agatatttca aaaataaacg gataataaag caaatccgaa aaaccgtcat aacaagattt
     5641 gaaaatgata tagtggttta aatttaaatc aggacaaggc gacgaagccg cagacagtac
     5701 aagcagtacg acaaggcgag gcaacgctgt actggttttt gttaaaccac tataagaatg
     5761 caaacgaaaa gcctcagtat tatccgagac aacggcaaac tttccatcgt cattcccgca
     5821 caagcgggaa tccaaaaaca aaaagccgca gaaatttaac ggaagaacca aaccccgaaa
     5881 accggctccc cgccttcgct tgaataacag aacaaaggaa tcagtattat ccgagacaac
     5941 cgcaaacttt ccatcgtcat tcccgca
//
LOCUS       DAWKOD010000122         5935 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000122 DAWKOD010000000
VERSION     DAWKOD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.122"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1276)
                     /locus_tag="WJK73_001617"
     CDS             complement(83..1276)
                     /locus_tag="WJK73_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7558076.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(1312..3171)
                     /locus_tag="WJK73_001618"
     CDS             complement(1312..3171)
                     /locus_tag="WJK73_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7558077.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            3445..4224
                     /locus_tag="WJK73_001619"
     CDS             3445..4224
                     /locus_tag="WJK73_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7558078.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4435..5847)
                     /locus_tag="WJK73_001620"
     CDS             complement(4435..5847)
                     /locus_tag="WJK73_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7558079.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT         1520 a         1686 c         1249 g         1480 t
ORIGIN      
        1 gccccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacggtttcc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttagcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaaatca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt ataatggtcc tgatccgcct
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaagatgc agtcggccgg
     1021 ttacgttgaa acgcgtgccg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatggtcgt cggccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg tgccgtcggg attggtctcc tgaatcttat
     1201 tgcctttttc atccagttca tatacatcga catatactgg cgtccggctg ccgctgtatt
     1261 cgtcatagta atacacctgc ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcattcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgcctgcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagaagacaa atcaccgtcc ggctcccctg
     1441 acaacttccc gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg
     1501 ttcgtagatt tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa
     1561 gaaatcatgc tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca
     1621 ttggcggtcg tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc
     1681 cttccgatta aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa
     1741 gatgccttcc ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccgg
     1801 atccccccaa gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc
     1861 aaaaccggct ttgcgttctg cggcacggcg gctgcggtac tgctcgaaag cagtattatc
     1921 gaaacggttt ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg
     1981 atatgctttc agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt
     2041 ttcggtcaaa aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag
     2101 aaaaaactgc cccaccttcg catcggattc attcttggta taagacactt cggcattgag
     2161 ctgccaaccg ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact
     2221 caaccgcgac caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca
     2281 ggcaggcgtt gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga
     2341 aaaatcagga acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc
     2401 gcccgcatcg gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt
     2461 aaacgatttt ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt
     2521 caggctgcct gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc
     2581 tccggtatgt ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg
     2641 ctctgaattg gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac
     2701 cgccaaatcg gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac
     2761 attgacggta ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg
     2821 cgtctgcaag cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc
     2881 aagcgtatgc accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg
     2941 cgtatccttg cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga
     3001 acgggtcttc tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccag
     3061 actgacggta ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc
     3121 cgataacagc ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc
     3181 tgtaatatgc aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat
     3241 taattgaaaa aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac
     3301 aaaaaacaaa ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata
     3361 tccgaattta aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg
     3421 acaaacacac tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa
     3481 aagattttgt gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc
     3541 gaatcctacc gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc
     3601 tttatttggc gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt
     3661 gccgtactca tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac
     3721 accatcctgc tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc
     3781 aaggatttgg tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa
     3841 caacctgaaa tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc
     3901 gaagaattga ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa
     3961 tacagacact cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta
     4021 agcggaaaac gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc
     4081 aagttcaccc cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat
     4141 actttccaaa cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa
     4201 atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg
     4261 gtcaaaccga cacggcacga aaaccgcatc ggcaaaaatg ccgtctgaaa aaattcagac
     4321 ggcatcttcg gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca
     4381 atcaatacga acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc
     4441 gatttcctta atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccggtaaatc
     4501 tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg ccgcccattc catgcagaac
     4561 aactttttcc cccgttaaag gatcagtttc ggtaatttct acctcatgca tttttcccca
     4621 atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccgcacgc
     4681 cttatcgcat cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg
     4741 aaggataccg gctgaggcag gacacaatcc gtatgtttcc gcccacgacg acaaagcccg
     4801 tctcgccgcc tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt
     4861 caaagccgct gttttcgcat tcaacatgcc gtctgaacct ttttcaaacc tgacggtcgt
     4921 aaatgtttta agcagatttt tggcagatac ataacaaccc gaatgataac gtccgaccaa
     4981 ttccgcttta atcttatatg catgcaggct gcccaatgca ggaaaaaaac ggacttcctc
     5041 cgtattgcac caatcaaacg gggcttttcc ggaatccaat aaagccgaaa cctcgctata
     5101 tacccgttca aacgtaccga tgtaatttac tttccctccg ccgtcgaaac aagccagcac
     5161 ccccataccg tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg
     5221 cagcggtttc ggattcatca aacggaaaca ctgcctgatc catgcctcaa ccccatgttc
     5281 cgacaaactg tattccaaat aatcacacaa tgccgacaca tccgccatcg cacgatgcct
     5341 gtcttctaca acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg
     5401 cggatacaga caccgggaca gttgcacact gcacaaagca ggcgatgaaa acccgatgcc
     5461 cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag
     5521 cacacaaccc ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc
     5581 ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg
     5641 aggattaacc aaccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac
     5701 ttcggttacc ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac
     5761 aggcattcca aaccgtaaaa ataccttttc cagcaagggc cagcgggaag caacaatcat
     5821 tttattctct ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc
     5881 aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgca
//
LOCUS       DAWKOD010000123         5928 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000123 DAWKOD010000000
VERSION     DAWKOD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.123"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            55..1593
                     /gene="murJ"
                     /locus_tag="WJK73_001621"
     CDS             55..1593
                     /gene="murJ"
                     /locus_tag="WJK73_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ7558080.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            1725..2681
                     /locus_tag="WJK73_001622"
     CDS             1725..2681
                     /locus_tag="WJK73_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ7558081.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2732..3514
                     /gene="trpC"
                     /locus_tag="WJK73_001623"
     CDS             2732..3514
                     /gene="trpC"
                     /locus_tag="WJK73_001623"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ7558082.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3580..5880
                     /gene="recQ"
                     /locus_tag="WJK73_001624"
     CDS             3580..5880
                     /gene="recQ"
                     /locus_tag="WJK73_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ7558083.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLGMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGKTGVMLRPLK
                     RNKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         1274 a         1768 c         1630 g         1256 t
ORIGIN      
        1 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaat
       61 atgctgggag ctttggtaaa agtcggcagc ctgacgatgg tgtcgcgcgt tttgggattt
      121 gtgcgcgata cggtcattgc gcgcgcattc ggcgcaggca tggcgacgga tgcgttcttt
      181 gtcgcgttca aactgcccaa cctgcttcgc cgcgtgtttg cggagggggc gtttgcccaa
      241 gcgtttgtgc cgattttggc ggaatataag gaaacgcgtt ctaaagaggc gacggaggct
      301 tttatccgcc atgtggcggg gatgctgtcg tttgtactgg tcatcgttac cgcgctgggc
      361 atacttgccg cgccttgggt gatttatgtt tccgcacccg gttttgccaa agatgccgac
      421 aaatttcagc tctctatcga tttgctgcgg attacgtttc cttatatctt attgatttca
      481 ctttcctctt ttgtcggctc ggtactcaat tcctatcata aattcagcat tcctgcgttt
      541 acgcccacgt tcctgaacgt gtcgtttatc gtattcgcgc tgtttttcgt gccgtatttc
      601 gatcctcccg ttaccgcgct ggcttgggcg gtttttgtcg gcggcatttt gcaactcggc
      661 ttccaactgc cctggctggc gaaactgggt tttttgaaac tgcccaaact gagtttcaaa
      721 gatgcggcgg tcaaccgcgt gatgaaacag atggcgcctg cgattttggg cgtgagcgtg
      781 gcgcagattt ctttggtgat caacacgatt ttcgcgtctt atctgcaatc gggcagcgtt
      841 tcatggatgt attacgccga ccgcatgatg gaactgcccg gcggcgtgct gggggcggca
      901 ctcggtacga ttttgctgcc gactttgtcc aaacactcgg caaaccaaga tacggaacag
      961 ttttccgccc tgctcgactg gggtttgcgc ctgtgcatgc tgctgacgct gccggcggcg
     1021 gtcggaatgg cggtgttgtc gttcccgctg gtggcaacct tgtttatgta ccgtgaattc
     1081 acgctgtttg acgcgcagat gacgcaacac gcgctgattg cctattcttt cggtttaatc
     1141 ggtttgatca tgattaaagt gttggcgccc ggcttctatg cgcggcaaaa catcaaaacg
     1201 cccgtcaaaa tcgccatctt cacgctcatc tgcacgcagt tgatgaacct tgcctttatc
     1261 ggcccgttga aacacgccgg gctttcgctc gccatcggtt tgggcgcgtg catcaatgcc
     1321 ggattgttgt tcttcctgtt acgcagacac ggcatttacc ggcccggcaa gggttgggcg
     1381 gcgttcttgg cgaaaatgct gctctcgctc gccgtgatgg gcggcggact gtgggcggcg
     1441 caggcttgcc tgccgtttga atgggtacgc gccggcggaa tgcggaaagc ggggcagctc
     1501 tgcatcctga ttgccgtcgg cggcggactg tatttcgcat cactggcggc tttgggcttc
     1561 cgtccgcgcc atttcaaacg cgtggaaaac tgaccgatgc cgatatcctt tttcggcaaa
     1621 cgcttttcca cgcctatatt cgggcgcgca tcccccacgt ccgcaccagc cggacgggct
     1681 gccgcatcat caatgccgtc tgaaaacaag aaaaccgata ccttatgatt ttgacaccgc
     1741 cggacacacc ctttttcctc cgcaacggca atgccgacac gattgccgcc aaattcctgc
     1801 aacgctccgc acctgcatac cgccgcgagc tgcttcccga cagcacgggt aaaaccaaaa
     1861 ccgcctacga cttttcagac ggcatttcgc ccgatgcgcc gctggtcgtg ctgtttcacg
     1921 gtttggaggg cggcagtggc agccattacg cggtcgaact gatgctcgcg gtacgcgatc
     1981 ggggttggaa cggcgtagtc gtccatttcc gcagctgcgg cggcgtagcg aacaccgccc
     2041 cggtgttcta ccacttgggc gataccgccg aaattgcctt tactttggac acgctcgccg
     2101 cgcgttaccg tgaaatatac gccgtcggcg tatcgctggg cggcaacgcg ctggcaaaat
     2161 atttgggcga acagggcgaa aacgcgctgc cgcaagccgc cgccgtcatc tccgcacccg
     2221 tcgatgcaga ggcggcaggc aaccgcttcg acagcggcat cacacggctg ctctacacgc
     2281 gctacttcct ccgcacactg atacccaaag cacggtcgct ccaaggtttt cagacggcat
     2341 ttgccgcagg gtgcaaaaca ctgggcgagt ttgacgaccg tttcaccgca ccgctgcacg
     2401 gctttgccga tcggcacgac tactaccgcc aaacttcctg caaaccgctg ctcaaacacg
     2461 ttgccaaacc gctgctcctg ctcaatgccg tcaacgaccc cttcctgccg cccgaagcgc
     2521 tgccccgcgc agacgaagtg tccgaagccg ttaccctgtt ccagccgaca cacggtggtc
     2581 atgtcggctt tgtcggcagc accggcggca ggctgcacct gcaatggttg ccgcagaccg
     2641 tcctgtccta tttcgacagc ttccgcacaa acaggcgtta acggtttgat gctaatattc
     2701 cccctttccc cagacgaata cggaacacga catgaccgac atcctcaata aaatccttac
     2761 cgccaaggca caggaagttg ccgcccaaaa agccgccgtc aacgccgaac acatccgcgc
     2821 acttgccgca gaagccgcgc ccgtccgcag ctttatcggt tcgatacgcg gcaaacaccg
     2881 cctcaacctg cccgccgtca ttgccgaaat caaaaaggca agcccgagca aagggttaat
     2941 ccgtccggac ttccgaccgc ccgagattgc acgcgcctat gaaaacgccg gggcggcgtg
     3001 tttgtccgta ctgaccgacg aaccctattt ccaaggttcg cccgaatacc tcaaacaggc
     3061 gcgcgaagcc gtatcgctgc ccgtgctgcg caaagacttc atcatcgacg aataccaggt
     3121 ttatcaggcg cgcgcctggg gggcggatgc cgtcctgctg attgccgccg cactggaacg
     3181 ggaacaattg gaacgctttg aagcggtggc gcacgaattg ggcatgaccg tcctgctcga
     3241 gctgcacgat gaaagcgaat tggaaaaatg ccgcaacctg accacgccgt tgtggggtgt
     3301 aaacaaccgc aacctgagga cttttgaagt ctccctcgac caaaccctgt cgctgctgcc
     3361 cgcgctggaa ggcaaaaccg tcgttaccga aagcggcatt acaggcaagg cggatgtgga
     3421 atttatgcaa tcgcgcggcg tgcatacctt cctgatcggc gaaacgttta tgcgtgccga
     3481 caatattgag gcggaagtag gcaaactctt ctaaatcccg atttcagacg gcattccctt
     3541 tctttaaccg acacaccctt taccggacac ggaaccatca tgcaccgacc gaccgccaaa
     3601 cagattctgc acgaagtatt tggttatccc gaatttcgcg gcaggcagga ggctgtcatc
     3661 aatactttgg caggcggcgg gagtttgacg gtgctgatgc cgacgggcgg gggcaagtct
     3721 ttgtgttacc agattcccgc gctgatgcgc gaaggcgtgg cggttgtcgt atcgccgctg
     3781 attgcgctga tgaacgacca agtggccagc ctgcatgtgg ccgggattga agcagcggca
     3841 gtcaacagtg gtacatcggc agatgaggcg cgcgagattg ccgacaagct tgcccaaggc
     3901 cgtctgaagc tgctttatgt cgcaccggaa cgcctagtta ccgaccgctt tttacgtttt
     3961 ctcgaccaac aaaccgtcag cctgtttgcc attgatgagg cgcattgcgt cagccagtgg
     4021 ggacacgatt tccgccccga ataccaacag ctcggcatgc ttgccgaacg ctatccgaac
     4081 gtcccgcgca tcgccctgac cgctaccgcc gatgccgcca cgcgcgccga catcaagcat
     4141 tatctgcact tggacgacgc gcccgaattt gtctccagct ttgaccgccc gaatatttat
     4201 tatcaggtta tcgaaaaaaa caacggcaaa aaacaactgc ttgaattcat ccgcaaagaa
     4261 atgacggggc aaagcggcat tgtgtattgc ttaagccgca aaaaggttga agatgtggcg
     4321 cggtttttgc gtgaaaacgg attaaacgcg attccgtatc acgccggttt gggtatggac
     4381 gtgcgtgagg aaaaccaacg ccgctttacg cgtgaagaca atattatcgt ggtggcgacc
     4441 gtggcgttcg gtatgggcat agacaaaccc gacgtgcgct ttgtcgccca tctcgatatg
     4501 ccccagagtg tcgaacattt ctatcaggaa tcggggcgcg ccggccggga cgggctgcct
     4561 gccgcgagct ggctgtgtta cggcttgaac gattgggtgt tgctgcgcga acggattgcc
     4621 gaaggcaaca gcggcgaagt gcaaaagcaa atcgaaatgc aaaaactcga tgccatgctt
     4681 gccgtctgcg aaaccgccgc ctgccgccgc gtgctgctgc tcaaacattt cggcgaagca
     4741 tccgaaccct gcggccattg cgacaactgc ctgcatccgc ccgtacggtt tgacggcacg
     4801 gtgttggtgc aaaaattact cagctgcgtg taccgcgccg gacaacgttt tgccgccggt
     4861 tacatcacca accttttgcg cggtaaaagc gacgattgga tacgcggcaa ccggcacgaa
     4921 caactgtcca cattcggcat cggtgcagaa ttgtccgaca aagaatggcg cagcgtcatc
     4981 cgccagtgta tcagcctcgg ctacctcacc gtcaacattg cccgatatca ggcattgcaa
     5041 ctgaccgaag ccgccaaaaa agtcctcaaa ggcaaaaccg gagtgatgct gcgtccgctc
     5101 aaacgcaaca agcccgccgt ccgcaccctc aaagacaact ggctgcgtac cgaacgcgaa
     5161 gaacgcctgt ggcaggcatt gcgcgtttgg cgtatgaaac aggcagaagc cgaaggcatc
     5221 cccgcctata tgattttcgg cgacaaaacc ctgcgcgacc ttgtcgaaaa aatgccgcaa
     5281 aacctcaacg ggctgcacga catctacggc ttgggcgaag ccaaaaccga acgtttcgga
     5341 cacggcatac tcgaagtctg ccgcaacgcc gccggcttca gtcgcgacgc ggtcatccgt
     5401 ccgcaaaccg aacgcgaaca acaactgcgt caaaaactcg aggcctggcg gtatgaacag
     5461 gcaagggcgg aaaactgcgc cctgcatgcc gtcctctccg acgaaagcct tgccgatatg
     5521 cttgccgcca cgcccgaaac cgaaaccgac ctcgaaggcg tacacggctt gggcagcgta
     5581 cgcgccgcca aatacggacg ggacatcctc gccgtctgcc gtccgttttc agacggcatc
     5641 gatgaagccg ccaaacgcaa acgccgcctg atgcgcgccc tgatccaatg gtgcggcgaa
     5701 acggcaaaac acgaacagtc cgaaccctac cgcattctca gcaaagccgc cctgcgcgcc
     5761 attgccgcca aacagccgaa gggtttggcg gagcttgccg ccgtatacgg cgtgggcgaa
     5821 gaaaaaaccg cacgttacgg tgcggcggtg ttggcggtgt tgggacaaaa tgccgtctga
     5881 aaaccgttac caagtttcag acggcattgc ctctatttaa aaattcct
//
LOCUS       DAWKOD010000124         5863 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000124 DAWKOD010000000
VERSION     DAWKOD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.124"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..454
                     /locus_tag="WJK73_001625"
     CDS             41..454
                     /locus_tag="WJK73_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7558084.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WJK73_001626"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WJK73_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ7558085.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WJK73_001627"
     CDS             1267..2766
                     /locus_tag="WJK73_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ7558086.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKDEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2968..4488
                     /locus_tag="WJK73_001628"
     CDS             2968..4488
                     /locus_tag="WJK73_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ7558087.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4545..5630
                     /gene="nagZ"
                     /locus_tag="WJK73_001629"
     CDS             4545..5630
                     /gene="nagZ"
                     /locus_tag="WJK73_001629"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ7558088.1"
                     /translation="MTVPCIPRGPVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5711..5787
                     /locus_tag="WJK73_001630"
     tRNA            5711..5787
                     /locus_tag="WJK73_001630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5745..5747,aa:Pro,seq:cgg)
BASE COUNT         1344 a         1783 c         1474 g         1262 t
ORIGIN      
        1 caatccgtcg ccgatttttg cctgtttgag actgtttgca atgatgcccg cctacgccta
       61 cgccgcgctc gctttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc accgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atggggcgtt tgctgccctc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tgcgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaacgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca aatccgccaa gaaatcttcg agcgtttccg
      541 cgctgccaac ccccatccta ccacagaact caacttcaac tcccctttcg agcttttaat
      601 cgccgtttta ctgtcggcac aggcaaccga cgtcggcgta aacaaagcga cggcgaagct
      661 gtttccggtt gccgatacgc cgcaggcgat gctggatttg ggtttggacg gcgtgatgga
      721 atatacgaaa accatcgggt tgtataaaac caagtccaaa cacatcatgc agacctgccg
      781 catcctgttg gaaaaataca agggcgaagt gccggaagac cgcgaggctt tggaatcgtt
      841 gcctggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acggcgttcg gacaccccgt
      901 tatggcggtc gatacgcata ttttccgcgt gtccaaccga accaaaatcg cccccgggaa
      961 agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt cctaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggctttaa agccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgtc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atctttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcagcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaaactcat cggttcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacccttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acttaaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcggg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaagacg aagaaatcac aatcaaagtc aagctgggca acgccgccga gcatatcggc
     2461 gcatcatcca aaacagatga agccccctac accgaacagc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggaca cctcgtcgtc
     2581 gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc gcggcgacga aattcttgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagcc ggtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctgat catgcgccgt ggcaacacgc tgtttatcgc attaaacctg
     2761 caataacaca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttacaaa aatcagttgg ttgattttaa acaaatattt tttacgacaa
     2881 aaaatatttc cgcccatttt gccaacaaaa cccaaaccgg aggcggacag atacgctccc
     2941 atccggacgc ttaacacccc cataaccatg cagctgatcg actattcaca ttcatttttc
     3001 tcggttgtgc cacccttttt ggcactggca cttgccgtca ttacccgccg cgtactgctg
     3061 tctttaggca tcggtattct ggtcggcgtt gcctttttgg tcggcggcaa ccccgtcgac
     3121 ggtctgacac acctgaaaga catggtcgtc ggcttggctt ggtcagacgg cgattggtcg
     3181 ctgggcaaac caaaaatctt ggttttcctg atacttttgg gtatttttac ttccctgctg
     3241 acctactccg gcagcaatca ggcgtttgcc gactgggcaa aacggcacat taaaaaccgg
     3301 cgcggcgcga aaatgctgac cgcctgcctc gtgttcgtaa cctttatcga cgactatttc
     3361 cacagtctcg ccgtcggtgc gattgcccgc cccgttaccg acaagtttaa agtttcccgc
     3421 accaaactcg cctacatcct cgactccact gccgcgccta tgtgcgtgct gatgcccgtt
     3481 tcaagctggg gcgcgtcgat tatcgccacg cttgccggac tgctcgttac ctacaaaatc
     3541 accgaataca cgccgatggg gacgtttgtc gccatgagcc tgatgaacta ttacgcactg
     3601 tttgccctga ttatggtgtt cgtcgtcgca tggttttcct tcgacatcgg ctcgatggca
     3661 cgtttcgaac aagccgcgtt gaacgaagcc cacgatgaaa ctgccgtttc agacgctacc
     3721 aaaggtcgtg tttacgcact gattattccc gttttggcct taatcgcctc aacggtttcc
     3781 gccatgatct acaccggcgc gcaggcaagc gaaaccttca gcattttggg ggcatttgaa
     3841 aacacggacg taaacacttc gctggtattc ggcggcactt gcggcgtcct tgccgtcgtt
     3901 ctctgcacgc tcggcacgat taaaaccgcc gactatccca aagccgtttg gcagggtgcg
     3961 aaatctatgt tcggcgcaat cgccatttta atcctcgctt ggctcatcag tacggttgtc
     4021 ggcgaaatgc acaccggcga ttacctctcc acactggttg cgggcaacat ccatcccggc
     4081 ttcctgcccg tcatcctctt cctgctcgcc agcgtgatgg cgtttgccac aggcacaagc
     4141 tgggggacgt tcggcattat gctgccaatt gccgccgcca tggcggtcaa agtcgaaccc
     4201 gcgctgatta tcccgtgtat gtccgcagta atggcggggg cggtatgcgg cgaccactgt
     4261 tcgcccatct ccgacacgac catcctgtcg tccaccggcg cgcgctgcaa ccacatcgac
     4321 cacgttacct cgcaactgcc ttatgccctg acggttgccg ccgccgccgc atcgggctac
     4381 ctcgcattgg gtctgacaaa atccgcgctg ttgggctttg gcacgacagg cattgtattg
     4441 gcggtgctga tttttctgtt gaaagataaa aaacgcgcca acgcctgacc cctttccctg
     4501 ttcagacggc atatgccgtc tgaagctttt tggagcaaac cgaaatgact gttccctgta
     4561 ttccgcgcgg ccccgtcatg gccgacattg ccgccttccg cctgaccgaa gaggaaaagc
     4621 aacgccttct cgatcccgcc gtcggcggag tcatcctgtt ccgccgcaac ttccaaaata
     4681 tcgcacagct caaagaactc accgccgaaa tcaaagccct gcgcacaccc gaactcatta
     4741 tcgccgtcga tcacgaaggc ggaagggtgc aacgtttcat cgaaggtttt acccgcctgc
     4801 ccgcaatgaa cgttttaggg caaatttggg acaaagacgg cgcgtccgcc gccgaaaccg
     4861 cagcccggca gataggctgg gttttggcaa ccgaactttc cgcctgcggc atcgacttgt
     4921 ccttcactcc cgtcttggat ttggactggg gaaactgcgc cgtcatcggc aaccgcagtt
     4981 tccaccgcaa tcccgaagcg gtcgcccgcc ttgccctcgc ccttcaaaaa ggtttggaaa
     5041 aaggcggtat gaaatcctgc ggtaaacatt ttcccggaca cggatttgtc gaaggagaca
     5101 gccatttggt gctacccgaa gacgggcgca gcctgtccga actcgaagcc gccgacctcg
     5161 cccccttccg cattatgagc cgcgaaggta tggcagcggt tatgcccgcc cacgtcgttt
     5221 atccgcaagt ggacacaaag cccgccgggt tttccgaaat ctggctcaaa caaattttgc
     5281 gccgcgacat cgggttcaaa ggcgtgattt tctcggacga tttaactatg gagggcgcgt
     5341 gcggggcagg cggcatcaaa gaacgcgccc gcatttcctt tgaggcaggt tgcgacatcg
     5401 tcctcgtctg caaccgtccc gaccttgtgg acgaactgcg cgatgatttc cgcattcctg
     5461 acaatcccgc tttggcgcaa cgttggcaat atatggccaa cacgctcggc agtgccgccg
     5521 cgcaagccgt tatacagacg gcggatttcc aagcggcgca agcctttgtt gccggccttg
     5581 cctcgccgca agacacggcg ggcggcgtaa aagtcggcga agccttttaa atccgcccga
     5641 cacgcggcaa aaaggcaaaa cggtataggc acgccgtatt ttttgcgcta taatcacgcc
     5701 ttacttaatt cggaatgtag cgcagcccgg tagcgcactt cgttcgggac gaaggggtcg
     5761 gaggttcgaa tcctctcatt ccgaccaaat ccaaaccgcc tcggtatcca ttgccgaggc
     5821 agtttttcct tttgacgtca acagaaaaat gccgtctgaa aca
//
LOCUS       DAWKOD010000125         5819 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000125 DAWKOD010000000
VERSION     DAWKOD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.125"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            334..5769
                     /gene="iga"
                     /locus_tag="WJK73_001631"
     CDS             334..5769
                     /gene="iga"
                     /locus_tag="WJK73_001631"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ7558089.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     ERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEAE
                     REAQALAVRRKAEAEGAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQSQPQR
                     RKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQR
                     AEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQALAARQ
                     QKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAELARRQ
                     EAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDYMAAS
                     QNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDENVSES
                     VDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVET
                     VSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGASLTRH
                     IAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVL
                     TYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYMAGDIGAGSLRSRLQTQQKANFNR
                     TSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLD
                     FAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLYRNVT
                     LNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1959 a         1396 c         1301 g         1163 t
ORIGIN      
        1 cgtcattccc acgaaccccc atctcgtcat taccacgaaa gcgggaatct aggacgcagg
       61 gttaagaaaa cctacatccc gtcattcccg cacaagcggg aatccaaaaa caaaaagtcg
      121 cagaaattta acggaagaac cgaaccccga aaatcggctt cccgccttcg cttgaataac
      181 agaacaaagg aagcggaaat aaaaacatcg ggattctttt tttaaattga caataaaaca
      241 ccaaaattaa tgatttacac aacaaaaaat agtcaacacc gccaaaccgg cgtaaaatac
      301 aagacattat cacccttaaa acggtaaaac cttatgaaaa ccaaacgttt taaaattaac
      361 gccatatcct tatccatctt tcttgcctat gcccttacgc catactcaga agcggcattg
      421 gtcagagacg atgtcgatta tcaaatattc cgtgactttg cagaaaacaa aggcaaattt
      481 tttgtcggcg caaccgattt atcagtgaaa aacaaacaag gtcaaaacat cggcaacgca
      541 ttatccaacg taccgatgat tgattttagc gttgcagatg tcaacagacg tacattaacc
      601 gtcatcgatc cccagtatgc cgtcagcgtc aaacatgtaa aaggagacga aatctcttat
      661 tacgggcatc acaatgggca cttagatgtt agcaatgatg aaaacgaata tcgttcagtt
      721 gcacaaaatg actacgaacc aaataaaaat tggcatcacg gtaatcaagg tcgtcttgaa
      781 gactacaaca tggcacgcct taataaattc gtaacagaag tcgcacctat tgcaccaacc
      841 agtgcaggcg gcggcgttga aacctataaa gataaaaacc gtttttctga gtttgtgcga
      901 gttggagcag gtacgcaatt tgaatataac agccgctaca atatgacaga gctttcacga
      961 gcttatcgtt atgccattgc aggtacgcct tatcaagacg tgaatgtcac atcgaatcta
     1021 aatcaagaag gcttgattgg cttcggtgat aattcaaaac atcattcccc agaaaaactc
     1081 aaagaagttt tatctcaaaa cgcattgact aactatgctg tgttaggcga tagcggttca
     1141 ccattatttg cttatgataa acaagaaaaa cgctgggtct ttttaggtgc ttacgattat
     1201 tgggcaggct atcaaaaaaa ctcttggcaa gaatggaata tctataaaaa agaatttgca
     1261 gatgaaatca aacaacgcga taacgccggc accatcaaag gttatggcga acatcattgg
     1321 aaaaccacgg gcacaaacag ccatatcggt tcgacagcgg taaggcttgc cggcaatgaa
     1381 aggggtgcaa acaacggaca gaatgttacc tttgaaaaca acggcacttt ggtattggat
     1441 caaaacatca accaaggcgc gggcggtctg tttttcaaag gcgattacac agtcaaaggt
     1501 ataaataatg acatcacttg gctaggtgcg gggattgatg ttgccgacgg caaaaaagtc
     1561 gtttggcaag tcaaaaatcc gaatggcgac agattggcga aaatcggcaa aggcacatta
     1621 gaaataaacg gcacaggcgt taaccaaggg caattaaaag tcggcgacgg tacggttatt
     1681 ctgaatcaaa aagccgattc aaaccaaaaa gtccaggctt tctcccaagt cggcattgtc
     1741 agcggacgcg gtacattggt attaaatagt tcaaatcaga ttaatcccga taacctatat
     1801 ttcggtttcc gtggcggtcg tttggatgcc aatggcaatg acttgacttt tgaacacatc
     1861 cgcaacgtgg atgaaggcgc gcgcattgtc aaccacaaca caggccacac ctccacaatc
     1921 acgctaacgg gtaaatcttt aattaccaat ccgaacagcc tttcggtaca tagtatacaa
     1981 aatgactatg atgaagatga ttatagttat tattaccgac caaggcgacc catcccacaa
     2041 ggcaaagatc tttattacaa aaactatcgt tattacgccc taaaatccgg cggcagcgtg
     2101 aacgcaccga tgcccgaaaa cggacaaacg gaaaataacg actggatttt aatgggcagc
     2161 acacaagaag aagccaagaa aaacgcgatg aaccacaaaa acaatcagcg tattagcggt
     2221 tttagcggtt tctttggcga agaaaacgga aaaggacata acggtgcatt aaaccttaat
     2281 ttcaacggca aaagcgcgca aaaccgtttc ttgttaacag gtggcactaa tttaaacgga
     2341 aaaataagcg taactcaagg caatgtcttg ttatcaggtc gtccaacacc acacgcaaga
     2401 gattttgtga acaaatcttc agcccgaaaa gacgcacatt tctccaaaaa caatgaagtc
     2461 gtatttgaag acgactggat aaaccgcaca ttcaaagcca cagaaattgc ggttaatcaa
     2521 tccgcatcat tctcttccgg cagaaatgta tccgacatca ccgccaacat caccgcaaca
     2581 gacaacgcca aagtaaattt gggttacaaa aacggcgatg aggtttgcgt gcgctcggac
     2641 tataccggtt atgttacctg caacacaggc aacttatccg ataaggcttt aaacagcttt
     2701 ggagcgacac agattaacgg caatgtgaat ttgagtcaga atgcggcatt gactttgggc
     2761 aaggctgcat tatgggggca aattcaaggg caaggaaaca gccgtgtcag cctaaatcaa
     2821 catagcaaat ggcatttgac cggcgacagc caagtacaca atctgtcatt ggcggatagc
     2881 catattcatt tgaacaacgc ttccgacgcg caaagtgcaa ataaatacca cacgctcaaa
     2941 atcaatcatt tatccggtaa cgggcatttt cattatctga cgcacttggc aaaaaatctt
     3001 ggggataaag tgcttgtgaa ggaatccgca tccggtcatt atcagctcca tgttcaagat
     3061 aaaacaggcg aacccaatca ggaagggctg aatctctttg atgcatcatc cgtacgagac
     3121 cgatcgcacc tttccgtttc cttggcgaat aaccatgtag atttaggcgc attgcgttat
     3181 acaatcaaaa cagaaaatgg cattacccga ttgtacaatc cttatgccga gaaccgccgc
     3241 cgagtcaaac cggccccgtc tcctgccaca aacacggctt ctcaagcaca aacggacagt
     3301 gcacaaattg ccaagcctca aaatatcgtc gtcgcaccgc ctagcccgca ggcaaatcaa
     3361 gccgaagaag caaaacgcca acaagcaaaa gcggagcaag tgaagcgtca gcaagcagaa
     3421 gcagagcgta aatcggcaga gttagcaaaa caaaaagcag aagccgaacg cgaagcaaga
     3481 gagctggcga cccgccaaaa agcagaacaa gaacgcagca gcgccgaact tgcacgtcga
     3541 catgaaaaag aacgcgaagc ggcagagttg tcggcaaaac aaaaagtcga agccgaacgt
     3601 gaggctcaag cattggcggt acgccgaaaa gcagaagccg aaggggcaaa acgccaagct
     3661 gcagagcttg cacgccggca tgaaaaagaa cgcgaagcgg cagagttgtc ggcaaaacaa
     3721 agggtcgggg aagaggaacg ccgtcaaaca gcccaatccc agccgcaacg ccgcaaacgc
     3781 cgtgccgcac cgcaggatta tatggcagct tcccaagacc gtcctaaacg ccgcggacac
     3841 cgttcggtac agcaaaacaa tgtggaaatt gcccaagccc aagcagaact tgcgcgccga
     3901 cagcaggaag aacgcaaagc ggcagagttg ttggcaaaac aaagggcaga agccgaacgt
     3961 gaggctcaag cattggctgc gcgccgaaaa gcagaagccg aagaggcaaa acgccaagct
     4021 gccgaacttg cacatcgcca ggaagcagaa cgcaaagcgg cagaattgtc ggcaaaccaa
     4081 aaagccgcag ctgaagccca agcattggcg gcacgccaac aaaaagccct tgcgcgtcaa
     4141 caggaagaag cacgaaaagc tgcagagtta gccgtcaaac aaaaagcgga aaccgaacgc
     4201 aaaaccgccg aacttgccaa gcaaagggca gcagccgaag cagcaaaacg ccagcaagaa
     4261 gctcgccaga ccgccgaact tgcacgtcgt caagaagcgg aacgccaagc agcagagttg
     4321 tcggcaaaac aaaaagccga aacagatcga gaagcggcag aatcggcgaa acgaaaagct
     4381 gaggaagaag agcaccgtca agcagcccaa tcccagccgc aacgccgaaa acgccgtgcc
     4441 gcacctcagg attatatggc agcttcccaa aatcgtccga aacgtcgcgg acgcagatct
     4501 actctgccgg caccgccctc gccatcattt gattcgtcag cttacgcagc acccagggcc
     4561 ttgcataatc cggactggta tgaaaatgac tatgaagaaa tccccttgga tgcgttggaa
     4621 gatgaaaatg tatctgaatc agttgacaca tcagacaaac agcctcaaga caatacggaa
     4681 cttcatgaaa aatatgaaaa tgattatgaa gaaatcccct tggatgcgtt ggaagacgaa
     4741 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat
     4801 gaaaaagttg agacagtgag tttgcaacca agagccgcgc agccgcgagc ccaagccgcc
     4861 actcagccgc aagcccaagc cgccgcgcaa gctgatgcag tcagcaccaa taccaactcg
     4921 gctttatctg acgcaatggc aagcacgcaa tctatcttgt tggatacagg tgcttcatta
     4981 acacggcaca ttgcacaaaa atcacgcgct gatgccgaaa aaaacagcgt ttggatgtcg
     5041 aacatcggtt atggccgtga ttatgcttcc gcacaatatc gccggtttag ttcgaaacgc
     5101 acgcaaacac aaatcggcat tgaccgcagc ttgtccgaaa atatgcagat aggcggcgta
     5161 ttgacttact ctgacagtca gcatactttt gatcaggcga gcggcaaaaa tacttttgtg
     5221 caagccaacc tttatggtaa gtattattta aatgatgctt ggtatatggc cggcgatatt
     5281 ggtgcaggca gcttgagaag ccggttacaa acgcagcaaa aagcaaactt taaccgaaca
     5341 agcatccaaa ccggccttac tttgggcaat acactgaaaa tcaatcaatt cgagattgtc
     5401 cccagtgcgg ggatccgtta cagccgcctg tcatctgcag attacaagtt gggtaacgac
     5461 agtgttaaag taagttctat gtcagtgaaa acactgacgg ccggactgga ttttgcttat
     5521 cggtttaaag tcggcaacct taccgtaaaa cccttgttat ctgcagctta ctttgccaat
     5581 tatggcaaag gcggcgtgaa tgtgggcggt aattccttcg tctataaagc agataatcaa
     5641 cagcaatatt cagcaggtgc cgcgttactg taccgtaatg ttacattaaa cgtaaatggc
     5701 agtattacaa aaggaaaaca attggaaaaa caaaaatccg gacaaattaa aatacagatt
     5761 cgtttctaaa atactaaatt catagcaaaa taaaatgccg tctgaactca ggcttcaga
//
LOCUS       DAWKOD010000126         5802 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000126 DAWKOD010000000
VERSION     DAWKOD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.126"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            312..2447
                     /locus_tag="WJK73_001632"
     CDS             312..2447
                     /locus_tag="WJK73_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7558090.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPKAQKDQGGYGFAMRLKRRNWYLQAKEDEVKLNESDWETTGLPTEPKKLP
                     LKQESVISKVQANNDDNNIYTSPYLTQSNHQNGNTGNGANLPKNEVTNYKDFKYVYSG
                     WFYKHARNEIIRENSSIKGAKNGDDGYIFYHGKEPSRQLPASGTVTYKGVWHFATDVK
                     KSQNFRDIIQPLKKQGDRYSGFSGDDDEQYSNKNESMLKDGQEGYGFTSNLEVDFNNK
                     KLTGKLIRNDKVTNATTGDKHTTQYYSLEAQVTGNRFNGKATATDKPGNGETKQHPFV
                     SDSSSLSGGFFGPKGEELGFRFLSDDQKVAVVGSAKTQDKDANGNTEAASGGTGAAAS
                     GGAAAMPSENGKLTTVLDAVELKSGGKEVKNLDNFSNAAQLVVDGIMIPLLPETSESG
                     KNQADKGKNGETEFTRKFDYTPESDKKDTQAGTPTNGAQTASNTAGDTNGKTKTYEVE
                     VCCSNLNYLKYGLLTRKTAGNTVGSGNGSQAAAQTDAQSMFLQGERTDEKEIPSEQNV
                     VYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVDFGAKKINGKLTAENRQAATFTIEG
                     DIEGNGFSGTAKTAESGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPG
                     DKQTKNATNASGNSSATVVFGAKRQQPVR"
     gene            2534..5281
                     /gene="tbpA"
                     /locus_tag="WJK73_001633"
     CDS             2534..5281
                     /gene="tbpA"
                     /locus_tag="WJK73_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ7558091.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDGSSNYVYFVVEEECESRNYETCKAN
                     PKKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEH
                     TQQTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGA
                     EYGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSAD
                     GSDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFG
                     SNLRHQDYYFQNAKRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTGQICLFGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QLEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
BASE COUNT         1642 a         1441 c         1481 g         1238 t
ORIGIN      
        1 tctgtcggtg cggaaactta tcgggtaaaa cggtttcttg agattttgcg tcttggattc
       61 ccacttttgt gggaatgacg cgattagagt ttcaaaattt attctaaata gctgaaactc
      121 aacgcactgg attcccgcct gcgtgggaat gacgggatat aggtttccgt gcggacgcgt
      181 tcggattcac gactgcgcgg gaatgacggg attttggtgt attccctaaa aaaaataaaa
      241 aagcatttgc aaatttgtta aaaataaata aaataataat ctttatcatt ctttaattga
      301 attaggttta tatgaacaat ccattggtaa atcaggctgc tatggtgctg ccggtgtttt
      361 tgttgagtgc ttgtttgggc ggaggcggcg gcagtttcga tcttgattct gtcgataccg
      421 aagccccgcg tcccgcgcca aagtatcaag atgtttcttc cgaaaagccg aaagcccaaa
      481 aagaccaagg cggatacggt tttgcgatga ggttgaaacg gaggaattgg tatctgcagg
      541 caaaagaaga cgaggttaaa ctgaacgaga gtgattggga gacgacagga ttgccgacag
      601 aacccaagaa actgccatta aaacaagaat ccgtcatttc aaaagtacaa gcaaacaatg
      661 acgacaacaa tatttacact tccccctatc tcacgcaatc aaaccatcaa aacggcaaca
      721 ctggcaacgg tgcaaacctg ccaaaaaacg aagtaacaaa ttataaagat ttcaaatatg
      781 tttattccgg ctggttttat aaacatgcta gaaacgaaat cataagagaa aacagctcaa
      841 ttaagggtgc aaagaacggc gacgacggct atatctttta tcacggcaaa gaaccttccc
      901 gacaacttcc cgcttctgga acagttacct ataaaggtgt gtggcatttt gcgaccgatg
      961 tcaaaaaatc ccaaaatttt cgcgatatta tccagccttt gaaaaaacaa ggcgacaggt
     1021 atagcggatt ttcgggcgat gatgatgaac aatattctaa taaaaacgaa tccatgctga
     1081 aagatggtca agagggttat ggttttacct caaatttaga agtggatttc aataataaaa
     1141 aattgacggg taaattgata cgcaatgata aagttacaaa cgctactact ggcgacaaac
     1201 ataccacgca atattacagc cttgaggctc aagtaacagg caaccgcttc aacggcaagg
     1261 caacggcaac cgacaaaccc ggcaacggcg aaaccaaaca acatcctttt gtttccgact
     1321 cgtcttcttt gagcggcggc ttttttggcc cgaagggtga ggaattgggt ttccgctttt
     1381 tgagcgacga tcaaaaagtt gccgttgtcg gcagcgcgaa aacccaagac aaagacgcaa
     1441 atggcaatac tgaggcggct tcaggcggca caggtgcggc agcatcgggc ggtgcggcag
     1501 ctatgccgtc tgaaaacggt aagctgacca cggttttgga tgcggtcgag ctgaaatctg
     1561 gcggtaagga agtcaaaaat ctcgacaact tcagcaatgc cgcccaactg gttgtcgacg
     1621 gcattatgat tccgctcctg cccgagactt ccgaaagcgg gaaaaatcag gcagataaag
     1681 gtaaaaacgg cgaaacagaa tttacccgca aatttgatta cacgccggaa agtgataaaa
     1741 aagacaccca agcaggtacg ccgacgaatg gggcgcaaac cgcttcaaat acggcaggtg
     1801 ataccaatgg caaaacaaaa acctatgaag tcgaagtctg ctgttccaac ctcaattatc
     1861 tgaaatacgg gttgctgaca cgcaaaaccg ccggcaacac ggtgggaagc ggcaacggca
     1921 gccaagccgc cgcccaaacg gacgcgcaga gtatgttctt acaaggcgag cgcaccgatg
     1981 aaaaagagat tccaagcgag caaaacgtcg tttatcgggg gtcttggtac gggcatattg
     2041 ccaacggcac aagctggagc ggcaatgctt cagataaaga gggcggcaac agggcggaat
     2101 ttactgtgga tttcggtgcg aaaaaaatta acggcaagtt aaccgctgaa aacaggcagg
     2161 cggcaacctt tactattgag ggcgatattg agggcaacgg tttttccggt acggcgaaaa
     2221 ctgctgagtc aggttttgat ctcgatcaaa gcaataccac ccgcacgcct aaggcatata
     2281 tcacagatgc caaggtgcag ggcggttttt acgggcccaa agccgaagag ttgggcggat
     2341 ggtttgccta tccgggcgat aaacaaacga aaaatgcaac aaatgcatcc ggcaatagca
     2401 gtgcaactgt cgtattcggt gcgaaacgcc aacagcctgt gcgataagca cggctgccga
     2461 acaatcaaga ataaggcctc agacggcacc gctctttccg ataccgtctg aaagcgaaga
     2521 gtagggaaac actatgcaac agcaacattt gttccgatta aatattttat gcctgtcgct
     2581 gatgaccgcg ctgcccgttt atgcagaaaa tgtgcaagcc ggacaagcac aggaaaaaca
     2641 gttggatacc atacaggtaa aagccaaaaa acagaaaacc cgccgcgata acgaagtaac
     2701 cggcctgggc aaattggtca aaaccgccga caccctcagc aaggaacagg tactcgatat
     2761 ccgcgacctg acgcgttacg accccggcat cgccgtggtc gaacaggggc gcggcgcaag
     2821 ctcgggttac tcgatacgcg gtatggacaa aaaccgcgtt tccttgacgg tggacggctt
     2881 ggcgcaaata cagtcctaca ccgcgcaggc ggcattgggc gggacgagga cggcgggcag
     2941 cagcggcgca atcaatgaaa tcgagtatga aaacgtcaaa gctgtcgaaa tcagcaaagg
     3001 ctcaaactcg gtcgaacaag gcagcggcgc attggcgggc tcggtcgcat ttcaaaccaa
     3061 aaccgccgac gatgttatcg gggaaggcag gcagtggggc attcagagta aaaccgccta
     3121 ttccggcaaa aaccgggggc ttacccaatc catcgcgctg gcggggcgca tcggcggtgc
     3181 ggaggctttg ctgatccaca cgggtcggcg cgcgggggaa atccgcgcac acgaagatgc
     3241 cggacgcggc gttcagagct ttaacaggct ggtgccggtt gaggacggca gcagcaacta
     3301 cgtctatttt gtggtcgaag aagaatgcga aagcagaaat tacgaaacgt gtaaagcgaa
     3361 tccgaaaaaa gaggttgtcg gcaaagacga acgtcaaacg gtttccaccc gagactacac
     3421 gggccccaac cgcttccttg ccgatccgct ttcatacgaa agccgatcgt ggctgttccg
     3481 cccgggtttt cgttttgaaa acaaacggca ctacatcggc ggcatactcg aacacacgca
     3541 acaaactttc gacacgcgcg atatgacggt tccggcattc ctgaccaagg cggtttttga
     3601 tgcaaattca aaacaggcgg gttctttgcc cggtaacggc aaatacgcgg gcaaccacaa
     3661 atacggcggt ctgtttacca acggtgaaaa cggtgcgctg gtgggtgcgg aatacggtac
     3721 gggcgtgttt tacgacgaga cgcacaccaa aagccgctac ggtttggaat atgtctatac
     3781 caatgccgat aaagacactt gggcggatta tgcccgcctc tcttacgacc gtcagggcat
     3841 cggtttggac aatcattttc agcagacgca ctgttctgcc gacggttcgg acaaatattg
     3901 ccgcccgagt gccgacaagc cgttttccta ctacaaatcc gaccgcgtga tttacgggga
     3961 aagccacagg ctcttgcagg cggcattcaa aaaatccttc gataccgcca aaatccgcca
     4021 caacctgagc gtgaatctcg ggtttgaccg ctttggctct aatctccgcc atcaggatta
     4081 ttattttcaa aacgccaaac gcgcctattc gttgaaaacg ccccctaaaa acggcaatca
     4141 aaacagcccc aacggcgaca agagcaaacc ctattgggtc agcataggcg ggggaaatgt
     4201 ggttacgggg caaatctgcc tctttggcaa caatacttat acggactgca cgccgcgcag
     4261 catcaacggc aaaagctatt acgcggcagt tcgggacaat gtccgtttgg gcaggtgggc
     4321 ggatgtcggc gcgggcttgc gctacgacta ccgcagcacg cattcggacg acggcagcgt
     4381 ttccaccggc acgcaccgca ccctgtcctg gaacgcaggc atcgtcctca aacctgccga
     4441 ctggctggat ttgacttacc gcacttcaac cggcttccgc ctgccctcgt ttgcggaaat
     4501 gtacggctgg cgggcgggtg ttcaaagcaa ggcggtcaaa atcgatccgg aaaaatcgtt
     4561 caacaaagaa gccggcatcg tgtttaaagg cgatttcggc aacttggagg caagttggtt
     4621 caacaatgcc taccgcgatt tgattgtccg gggttatgaa gcgcaaattc aaaacggcaa
     4681 agaaacagcc aaaggcgacc cggcttacct caatgcccaa agcgcgcgga ttaccggcat
     4741 caatgttttg ggcaaaatcg attggaacgg cgtatgggat aaattgcccg aaggttggta
     4801 ttctacattt gcctataatc gtgtccgtgt ccgtgacatc aaaaaacgcg cagaccgcac
     4861 cgatattcaa tcacatctgt ttgatgccat ccaaccctcg cgctatgtcg tcggcttggg
     4921 ctatgaccaa ctggaaggca aatggggtgt gaacggtatg ctgacttatt ccaaagccaa
     4981 ggaaatcaca gagttgttgg gcagccgggc tttgctcaac ggcaacagcc gcaatacaaa
     5041 agccaccgcg cgccgtaccc gcccttggta tattgtggat gtgtccggtt attacacgat
     5101 taaaaaacac tttaccctcc gtgcgggcgt gtacaacctc ctcaactacc gctatgttac
     5161 ttgggaaaat gtgcggcaaa ctgccggcgg cgcagtcaac caacacaaaa atgtcggcgt
     5221 ttacaaccga tatgccgccc ccggccgcaa ctacacattt agcttggaaa tgaagtttta
     5281 aacgtccaaa cgctgcaaat gccgtctgaa aggcttcaga cggcattttt tacacaattc
     5341 ccaccgtttc ccatcatccc cgatacaccg taatcctgaa atccgtcatt cccgagcagg
     5401 cgggaatcca gtccgttcgg tttcggtttt tttgaggttt cgggtaactc taaaccgtca
     5461 ttttctcgaa agcggaaaat caaaaacaga aatctaaaat cccgtcattc ccgcgaaagt
     5521 gggaatctag acctgtcggt gcggaaactt atcggataaa acggtttctt tagattttac
     5581 gttctagatt cccgctttcg cgggaatgac gggatgcagg tttctgttcg gatgtattcg
     5641 gattccctcc tgcgcgggaa tgatggggtt tttaaatgtc cgaatgccgc aaatgccgtc
     5701 tgaaaggctt cagacggcat tttttacaca attcccaccg tttcccatcc ttctcgatac
     5761 actgtaatct caaatcccgt cattcccgcg caggcgggaa tc
//
LOCUS       DAWKOD010000127         5791 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000127 DAWKOD010000000
VERSION     DAWKOD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.127"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            132..566
                     /locus_tag="WJK73_001634"
     CDS             132..566
                     /locus_tag="WJK73_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ7558092.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(579..1442)
                     /locus_tag="WJK73_001635"
     CDS             complement(579..1442)
                     /locus_tag="WJK73_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7558093.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1450..2067)
                     /locus_tag="WJK73_001636"
     CDS             complement(1450..2067)
                     /locus_tag="WJK73_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ7558094.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2230..2844)
                     /locus_tag="WJK73_001637"
     CDS             complement(2230..2844)
                     /locus_tag="WJK73_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ7558095.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            3050..4822
                     /locus_tag="WJK73_001638"
     CDS             3050..4822
                     /locus_tag="WJK73_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ7558096.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            4819..5475
                     /locus_tag="WJK73_001639"
     CDS             4819..5475
                     /locus_tag="WJK73_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7558097.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGRSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1426 a         1629 c         1345 g         1391 t
ORIGIN      
        1 gttccgattc cgccgttatg tctgcctgcc ggacatacgt acgcattatt aacaaaagtt
       61 aaccgcgata ataccatctt tcacacgtca atctagtata tttcctaaaa tttccaacaa
      121 gaggaaaagc cgtgccactg cctgctccct gccgttttgc caaacctgcc gcctcttttt
      181 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccctga
      241 acgattttca accgaactgc gacatacgcc ggctcggact gacacagggt cagcacaatg
      301 aactgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
      361 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcctcg gatgttttta
      421 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
      481 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
      541 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
      601 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgaacg gctgccccgc
      661 gtctgcgccc actgcaatgc cgccatctgt gcggtttcat catacggcac gccgaaatct
      721 tccagccaat tttgcaccgc cgccagataa tcgttttgat cgaacggata aaaactgagc
      781 cacaatccga atcggtcgga cagggatatt ttttcttcca ccgcttcttt ctgatggatt
      841 tccccccgca tccccgtcgt accggcattc tcgtcaaaat attcgggcat caggtgccgt
      901 ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt gagacagacc gccgtctaac
      961 gcggttttca atgccttata ggtttcatcg ccgctttcaa acgacaaatc gtcgcaaaat
     1021 acgataaatt tttccggacg ttctttcaaa agcgtcaaca ggtaaggcag gccgattaaa
     1081 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg cagcagggct
     1141 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcgggt
     1201 ctgccggcaa tgaattgttc ggtattacgc accagcaatt cggtttgcct gccaactccc
     1261 gccagccttg ccaaggggaa ggtgtgcgga tcgggcaagt gttccaaaaa accttttttg
     1321 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
     1381 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     1441 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtat cgacgattgc
     1501 ctgcgtattc ggatcgattt cgatgttgat tctgtcgccg acctttctgc tgccgaacag
     1561 cgtccgttcc aaagtttcgg gaatcaaatg gacattgaaa cggctgtctt cgactttgcc
     1621 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     1681 ttcgtgcggg agcgaaaacc aaaccgtgcg gttgtacccg tcccgttcga tttcgacaat
     1741 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     1801 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccctaaat tggtttttgc
     1861 caaagtttcc gccattaaat cgaaactgac gcggtttcct tcgatttcgg taatcgtcag
     1921 gcagcagccg ttgttggcga ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     1981 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     2041 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     2101 aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     2161 tccgattcgg gcaaaaaccg cccggtacgg cttgaccttt cctttccacg ccggtcggcg
     2221 gtcttaccct tatccctcct gcaaatcagt ttgcgtgttc aagtcggcaa aatgcccgtc
     2281 aaactcgaat ctgaccggtc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     2341 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     2401 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     2461 cacggtttca aacctcgcca ccaaatcgtc cggcagatac ggcatatcgc acggcacaac
     2521 caaaagccag tcggcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     2581 cgggccgaaa tgctgccact gccgtgcgtc cggaaaaata tgcggacttc ttcgagcata
     2641 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     2701 gatgacatga tctatcagtg ccttaccccc caaaagagca agccccttgt cctcgcctcc
     2761 catacggctc gcctgaccgt cggccagtat cagggcaaaa gttttcattg cggatgttct
     2821 cttggaaaag ttcgaggttt tcatgattgc agtctgccgt tcccaatgac tcaaaatgcc
     2881 gtctgaagca gacggcaaat aaattcatat tatctgaatt ttatcataac atgatttaac
     2941 actgaagcgg tgcggattaa gttttcctta accaattctt tctgagcagt tgtatctaat
     3001 tccaaagaat gatattgttt gcattatttg gaacaatttt tcgccgagca tgatactgcc
     3061 agcccgtttt tcagacggca tcagcctttc cctacgcctg aaactcctga ccggagtgtg
     3121 ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg ctctctttgc gtctggaaaa
     3181 cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg caggcatacc gtctggcata
     3241 catggcgggt gaaggctcgc cccgtgcgca aattgacaat caggttgccg aatttgaaaa
     3301 aagcctgaat cgtatttcac aaagtgatgc catccatccg ctgattcctt cggacacccc
     3361 tcttgcttat gatttgatac aatccatgct gattatagat tggcaggcac acatcctccc
     3421 cccgctccag tcctaccggc gaccgactca ggtcgatctc taccgctttg ccggaaacat
     3481 cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa aacacatggt ggctcaggcg
     3541 ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct gtactgatgc tgttttggca
     3601 ccagatttgg gttatccggc cgctgcaggc gttaagggaa ggtgcggaac gcatcggacg
     3661 gaggtgtttc gatattccgg ttcccgaagg cggtacgccg gaattcaaac aggtcgggcg
     3721 ttgtttcaat caaatgggcg gcaggttgaa aattttatat gatgatttgg aaggacaagt
     3781 cgccgagcag acacgcagtc tcgaaaaaca aaatcaaaac ctgaccctgc tgtaccaaac
     3841 tacacgggac ctgcaccaat cctacatacc gcaacaggct gcagaacatt ttctaaaccg
     3901 tatcctgccc gccgtaggag cagattccgg cagagtttgt ttggacggcg gatccgatgt
     3961 ttatgtttcc attcatcatg cggattgcgg cacagcagct tcggatttgg ggaagtacca
     4021 tgaggaaatc ttccccattg agtaccagaa cgaaacattg ggcaggctgt tgctcagctt
     4081 tccaaacggc atttctcttg atgaagacga ccgcatcctg cttcaaacac taggcaggca
     4141 attgggcgta tcgcttgccg gcacaaaaca ggaggaagaa aaacgcctgc ttgcagtatt
     4201 gcaggaacgc aacctgattg cgcaaggatt acatgacagc atcgcacaag cattaacgtt
     4261 cctaaaccta caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc
     4321 cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga
     4381 actgctgctc aacttccgta ccaaaatcag caataaagaa tttcccgaag ccgttgccga
     4441 cctattcgcc cgctttacgc aacaaaccgg gataacggtc gaaaccgcct gggaaaacgg
     4501 ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt tttatcctgc aggaaagcct
     4561 gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaacacgg
     4621 cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg
     4681 agaacctacg ggcagccatg tcggactgca tatcatgcag gagcgtgcca aacgtatccg
     4741 cgccgtattg aaaatctatt cccaacccca acagggaacc accgtctcat tggcgggtgt
     4801 atctgaagaa agcttgaaat gaccattaaa attatcctga tagacgacca taccctcttc
     4861 cgcagcggca ttaaatccct tttgtcgcgc caacatgatt ttgaggtcat cggcgaagcc
     4921 gcagacggcc tctcgggcat taaaatgatc aaccggctcc aacccgatgt cgtcctgctt
     4981 gaccttgata tgcccggtat gaacggacgc gaagcactct cccaaatcat cagcatcaat
     5041 ccgcagcagg cagtgattat gctgaccgtt tccgaagaca acgacgactt gaccgaatgt
     5101 atgcgcatcg gcgcgcgcgg ctacctgctg aaaaacatca acgccgactt tctgctcgaa
     5161 agcatacgca aagccgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc
     5221 gttaaaagcc tgatttcccc ccaacctgcc caagggacac aggcactctc ctcacttacc
     5281 cctcgcgaac tggaaatctt gggctatctc gccgcaggac gcagcaacaa aatcatcgcc
     5341 cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa
     5401 ctcaacctca gcagccgggt gcaggccgct gtttacgcca tccggcacaa cgtcccccaa
     5461 cccgtgctgg aataaggcgg gttcagacgg catattaggg ggtttaatcc ccgtacggtc
     5521 attcggataa cagaccaagc atgtaagctt atgcccccat aagtacgctt ggcatagcag
     5581 taatattgtt cggtttagtg ttttccgttt gcccctatct gatactgcaa tatcagctat
     5641 gccgtctgaa aacgcatcat catgatattt tcagacggca taataaaaag cggaaatact
     5701 aatgcagggt aaaatgttcc attccgaatc ccataaatat acaatggctt atatcgttta
     5761 agcatgtgta acccaccctc atatcaatca a
//
LOCUS       DAWKOD010000128         5769 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000128 DAWKOD010000000
VERSION     DAWKOD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.128"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..306)
                     /locus_tag="WJK73_001640"
     CDS             complement(<1..306)
                     /locus_tag="WJK73_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ7558098.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     "
     gene            complement(342..455)
                     /gene="rrf"
                     /locus_tag="WJK73_001641"
     rRNA            complement(342..455)
                     /gene="rrf"
                     /locus_tag="WJK73_001641"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(552..3443)
                     /locus_tag="WJK73_001642"
     rRNA            complement(552..3443)
                     /locus_tag="WJK73_001642"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3850..3925)
                     /locus_tag="WJK73_001643"
     tRNA            complement(3850..3925)
                     /locus_tag="WJK73_001643"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3890..3892),aa:Ala,seq:tgc)
     gene            complement(3932..4008)
                     /locus_tag="WJK73_001644"
     tRNA            complement(3932..4008)
                     /locus_tag="WJK73_001644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3972..3974),aa:Ile,seq:gat)
     gene            complement(4110..5650)
                     /locus_tag="WJK73_001645"
     rRNA            complement(4110..5650)
                     /locus_tag="WJK73_001645"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1252 a         1694 c         1240 g         1583 t
ORIGIN      
        1 ttccgcaatg gccttcgcct tcgcagggcc tatgcccggc agcgcctcca gctcctgctg
       61 cgaagccgca ttgatgttta ccgccgcaag ggagaaggcg caggagaaca gcatacagaa
      121 cagcacgaac attttcttca tggtttttcc tttaagggtt gcaaacaata aaccgcatct
      181 tgcgacgata aaacgggtca ttctaaaatg aatatcccaa agtttcaagc cgttcctccg
      241 caaacccgac cggacgccgt acggatgccg tcccgccatc cccgacattt tttccgggca
      301 aagcaaaaac ccccgaataa tcgggggttt tctgaatggg tgtttggcag tgacctactt
      361 tcgcatggaa gaaccacact atcatcggcg ctgagtcgtt tcacggtcct gttcgggatg
      421 ggaaggcgtg ggaccaactc gctatggccg ccaaacttaa actgttacaa atcggtaaag
      481 ccttaatcaa tatattcggt aatgactgaa tcagtcagta agcttttatc tcttgaagtt
      541 cttcaaatga tagagtcaag cctcacgagc aattagtatg ggttagcttc acgcgttacc
      601 gcgcttccac accccaccta tcaacgtcct ggtctcgaac gactctttag tgcggttaaa
      661 ccgcaaggga agtctcatct tcaggcgagt ttcgcgctta gatgctttca gcgcttatct
      721 cttccgaact tagctacccg gctatgcaac tggcgttaca accggtacac cagaggttcg
      781 tccactccgg tcctctcgta ctaggagcag cccccgtcaa acttccaacg cccactgcag
      841 atagggacca aactgtctca cgacgtttta aacccagctc acgtaccact ttaaatggcg
      901 aacagccata cccttgggac cgactacagc cccaggatgt gatgagccga catcgaggtg
      961 ccaaactccg ccgtcgatat gaactcttgg gcggaatcag cctgttatcc ccggagtacc
     1021 ttttatccgt tgagcgatgg cccttccata cagaaccacc ggatcactat gtcctgcttt
     1081 cgcacctgct cgacttgtcg gtctcgcagt taagctacct tttgccattg cactatcagt
     1141 ccgatttccg accggaccta ggtaaccttc gaactcctcc gttacgcttt gggaggagac
     1201 cgccccagtc aaactgccta ccatgcacgg tccccgaccc ggatgacggg tctgggttag
     1261 aacctcaaag acaccagggt ggtatttcaa ggacggctcc acagagactg gcgtctctgc
     1321 ttctaagcct cccacctatc ctacacaagt gacttcaaag tccaatgcaa agctacagta
     1381 aaggttcacg gggtctttcc gtctagcagc gggtagattg catcttcaca accacttcaa
     1441 cttcgctgag tctcaggagg agacagtgtg gccatcgtta cgccattcgt gcgggtcgga
     1501 acttacccga caaggaattt cgctacctta ggaccgttat agttacggcc gccgtttact
     1561 ggggcttcga tccgatgctc tcacatcttc aattaacctt ccagcaccgg gcaggcgtca
     1621 caccctatac gtccactttc gtgttagcag agtgctgtgt ttttaataaa cagtcgcagc
     1681 cacctattct ctgcgaccct ccggggctta cggagcaagt ccttaacctt agagggcata
     1741 ccttctcccg aagttacggt atcaatttgc cgagttcctt ctcctgagtt ctctcaagcg
     1801 ccttagaatt ctcatcctgc ccacctgtgt cggtttgcgg tacggttcga ttcaaactga
     1861 agcttagtgg cttttcctgg aagcgtggta tcggttgctt cgtgtccgta gacactcgtc
     1921 gtcacttctc ggtgttaaga agacccggat ttgcctaagt cttccaccta ccggcttaaa
     1981 caagctattc caacagcttg ccaacctaac cttctccgtc cccacatcgc atttgaatca
     2041 agtacaggaa tattaacctg tttcccatcg actacgcatt tctgcctcgc cttaggggcc
     2101 gactcaccct acgccgatga acgttgcgca ggaaaccttg ggctttcggc gagcgggctt
     2161 ttcacccgct ttatcgctac tcatgtcaac attcgcactt ctgatacctc cagcacactt
     2221 tacaatgcac cttcatcggc ctacagaacg ctcccctacc atgccggtaa accggcatcc
     2281 gcagcttcgg ttatagattt gagccccgtt acatcttccg cgcaggacga ctcgaccagt
     2341 gagctattac gctttcttta aatgatggct gcttctaagc caacatcctg gctgtctggg
     2401 ccttcccact tcgtttacca cttaatctat cattggggac cttagctggc ggtctgggtt
     2461 gtttccctct tgacaacgga cgttagcacc cgctgtctgt ctcccgagga accacttgat
     2521 ggtattctga gtttgccatg ggttggtaag ttgcaataac cccctagcca taacagtgct
     2581 ttacccccat cagtgtcttg ctcgaggcac tacctaaata gttttcgggg agaaccagct
     2641 atctccgagt ttgtttagcc tttcacccct atccacagct catccccgca ttttgcaaca
     2701 tgcgtgggtt cggtcctcca gtacctgtta cggcaccttc aacctggcca tggatagatc
     2761 actcggtttc gggtctacac ccagcaactc atcgccctat taagactcgg tttccctacg
     2821 cctcccctat tcggttaagc tcgctactga atgtaagtcg ttgacccatt atacaaaagg
     2881 tacgcagtca cacctctagg gcgctcccac tgtttgtatg catcaggttt caggttctgt
     2941 ttcactcccc tcccggggtt cttttcgcct ttccctcacg gtactggttc actatcggtc
     3001 gatgatgagt atttagcctt ggaggatggt ccccccatat tcagacagga tttcacgtgt
     3061 cccgccctac ttttcgtacg cttagtacca ctattgagat ttcgaatacg ggactatcac
     3121 ccactatggt caagcttccc agcttgttct tctatctcga cagttatcac gtacaggctc
     3181 ctccgcgttc gctcgccact acttgcggaa tctcggttga tttcttttcc tccgggtact
     3241 tagatggttc agttctccgg gttcgcttct ctaagtctat gtattcaact taggatactg
     3301 cacagaatgc agtgggtttc cccattcgga catcgcggaa tcattgcttt attgccagct
     3361 cccccgcgct tttcgcaggc ttacacgtcc ttcgtcgcct atcatcgcca aggcatccac
     3421 ctgatgcact tattcacttg actctatcat ttcaagaact tctttgactt tgcctaacat
     3481 tccgttgact agaacatcag acttgaattt cctactttga taaagcttac tgctttgttg
     3541 tgtcttaatc ctgccttttg tgtttcagga ttaagtcgat acaatcatca cccaaatact
     3601 gtgtttgttt tcttttctct tgtgagcgat tttttatcct ttgcaaagaa taaaaaatca
     3661 aaacaaacgc tttgtctttg tttgttgatt tcggctttcc aatttgttaa agatcgatgc
     3721 gttcgatatt gctatctact gtgcaaatca aaacgagctg attattatat cagcattttg
     3781 ttcttggtca agtgtgacgt cgccctgaat ggattctgtt ccattcttcc gttttgattt
     3841 gtacagtatt ggtggaggca aacgggatcg aaccgatgac cccctgcttg caaagcaggt
     3901 gctctaccaa ctgagctatg ccccccgttc ttggtgggtc tgggaggact tgaacctccg
     3961 accccacgct tatcaagcgt gtgctctaac cagctgagct acaaacccgg attctcttct
     4021 taagcgaatc ttgccttcac tcaagcttct tccgcatctt tttcagttta ccgataagtg
     4081 tgaatgccta aagcctcttc tttctctaga aaggaggtga tccagccgca ggttccccta
     4141 cggctacctt gttacgactt caccccagtc atgaagcata ccgtggtaag cgggctcctt
     4201 gcggttaccc tacctacttc tggtatcccc cactcccatg gtgtgacggg cggtgtgtac
     4261 aagacccggg aacgtattca ccgcagtatg ctgacctgcg attactagcg attccgactt
     4321 catgcactcg agttgcagag tgcaatccgg actacgatcg gttttgtgag attggctccg
     4381 cctcgcggct tggctaccct ctgtaccgac cattgtatga cgtgtgaagc cctggtcata
     4441 agggccatga ggacttgacg tcatccccac cttcctccgg cttgtcaccg gcagtctcat
     4501 tagagtgccc aactgaatga tggcaactaa tgacaagggt tgcgctcgtt gcgggactta
     4561 acccaacatc tcacgacacg agctgacgac agccatgcag cacctgtgtt acggctcccg
     4621 aaggcactcc tccgtctccg gaggattccg tacatgtcaa gaccaggtaa ggttcttcgc
     4681 gttgcatcga attaatccac atcatccacc gcttgtgcgg gtccccgtca attcctttga
     4741 gttttaatct tgcgaccgta ctccccaggc ggtcaatttc acgcgttagc tacgctacca
     4801 agcaatcaag ttgcccaaca gctaattgac atcgtttagg gcgtggacta ccagggtatc
     4861 taatcctgtt tgctacccac gctttcgggc atgaacgtca gtgttatccc aggaggctgc
     4921 cttcgccatc ggtattcctc cacatctcta cgcatttcac tgctacacgt ggaattctac
     4981 ctccctctga cacactcgag tcacccagtt cagaacgcag ttcccaggtt gagcccgggg
     5041 atttcacatc ctgcttaagt aaccgtctgc gcccgcttta cgcccagtaa ttccgattaa
     5101 cgctcgcacc ctacgtatta ccgcggctgc tggcacgtag ttagccggtg cttattcttc
     5161 aggtaccgtc atcagccgct gatattagca acagcctttt cttccctgac aaaagtcctt
     5221 tacaacccga aggccttctt cagacacgcg gcatggctgg atcaggcttg cgcccattgt
     5281 ccaaaattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt cccagtgtgg
     5341 cggatcatcc tctcagaccc gctactgatc gtcgccttgg taggccttta ccccaccaac
     5401 tagctaatca gatatcggcc gctcgaatag cgcaaggccc gaagatcccc tgctttctct
     5461 ctcaagacgt atgcggtatt agctgatctt tcgatcagtt atcccccact actcggtacg
     5521 ttccgatatg ttactcaccc gttcgccact cgccacccaa gaagcaagct tctctgtgct
     5581 gccgtccgac ttgcatgtgt aaagcatgcc gccagcgttc aatctgagcc aggatcaaac
     5641 tcttatgttc aatctctaac tttttaactt ctggtctgct tcaaagaaac cgacaggaca
     5701 atgttcaaaa cattatcttg tctgtctttc aaacagtgtg aggctcaagg cactcacact
     5761 tatcggtaa
//
LOCUS       DAWKOD010000129         5669 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000129 DAWKOD010000000
VERSION     DAWKOD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.129"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            78..647
                     /locus_tag="WJK73_001646"
                     /pseudo
     CDS             78..647
                     /locus_tag="WJK73_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            879..1379
                     /locus_tag="WJK73_001647"
     CDS             879..1379
                     /locus_tag="WJK73_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558099.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFRRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            1386..1901
                     /locus_tag="WJK73_001648"
     CDS             1386..1901
                     /locus_tag="WJK73_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ7558100.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            1921..3399
                     /gene="ubiD"
                     /locus_tag="WJK73_001649"
     CDS             1921..3399
                     /gene="ubiD"
                     /locus_tag="WJK73_001649"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ7558101.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETEREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(3463..5577)
                     /locus_tag="WJK73_001650"
     CDS             complement(3463..5577)
                     /locus_tag="WJK73_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ7558102.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIGRRLLPRPTVILKNLTITEPGSNRTAVSVKETKIGLGWK
                     NLWSDQIQIEKWVVSGAELALTRDRKGVWNIQDLIDNPKRKASVNRIIIENSTVRLDL
                     PQDRLTLKEVSLNLQSPDSAGQQFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEAETALNGHRIAVSTTGSPTIRFNAGGADAADLALRADTAFHNLHLTAQIPALALR
                     NNGIKIETANGTFTAAGENSQWGGSFKLDRADLHPGIGNIGNAEISGNVKTPRLQTNF
                     SLGSPLVWSRDNGLEVPRLHISTLQDTVNRLPQPRFISRLDGSMSILNLQNWNAELNG
                     TFDRQPVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
BASE COUNT         1193 a         1392 c         1698 g         1386 t
ORIGIN      
        1 atttgcagtg tgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc
       61 ctaaaattta atccactata gtttatgcct cgcacgacgg ggaaatggcg cgcagtttcg
      121 atttgtggtt ggaactgttg aaatcgcaaa ccgtgtcgcc gacttcgttc ggtttgtcgg
      181 tgcacaatgc aacggcgggg taatggtcga tattgaggcg ggatatgagc gagcagacgg
      241 cattggccgt gtgtgccgac ggtgtggaaa cggcattggc ggaagcggca tcactgttgg
      301 aagagggatg cgggtcggtt ttggttttgg cggcggacga tccgctgccg gaaggatatg
      361 ccgtatcggc aacgcgcgcg ccgtttgctt atgctttggc gatggttctg accaaaggga
      421 cgcgttacag cctgacttta tctgcttcgg acgatatgcc gtctgaagcc ggtatgctgc
      481 ccgaggcata ttggagcggt ttggaatggg tgcgctttct gctgaacggc agtcgggaat
      541 gccgccgggt atatcgcaat cgtgaatggc tgtggcagcc cgcaagctgc aggctgaaga
      601 tttccgcaat gtccgcaccg tcaacgacgt ggtacaggcg gtattgaaga ttcaggcggg
      661 ctaatcgggt atttctaatt atttttcaga cggcatgttt ttatcggatg ccgtctgaaa
      721 acatttgtgc ggattcaaaa tgaaacccgg ttttttcgga catatttttc tgaccgtatt
      781 aagcattgcc tatcccgctt tgtggtatag tggattaaca aaaatcagga caaggcgacg
      841 aagccgcaga cagtacggca aggcgaggca acgctgtact ggtttttgtt aatccactat
      901 attacggcag ggaggcgggc tggtttgtct ggctggcggc ggcgatgtgc gggttgtggc
      961 tgctgcgcgc cctgactgcc aaactgcccc aacagcgtta tgcggctttg tttattacac
     1021 tgttttttgc cgcagtattg gtgttcagac ggcatgattc gatgtattgg tatcccgtcc
     1081 tcgtcaatct gatgatgttg gcggtgttcg gcggcagcct gtttgccgga cagacggtca
     1141 tagaaaggct cgcgcggctg caacagcccg acctgcctga aaaagccgtg cgctataccc
     1201 gccgggtaac gcaggtttgg tgcgtattct ttattgccaa cgggacgctt gccgctttgt
     1261 tggcacggtt ggggcgatat gattggtggg cggtttatac cggcgtgatt gcctatgtat
     1321 tgatggggat gccgtttgcc ggcgaatggc tttaccgcaa actcgttttg aaagtctgaa
     1381 accttatgta cacttgcact tcctgcggcg aaaaacatga agatatgccc gcaatcggct
     1441 ttaccgcccc cgatccctat aatcaactca gtgaagaaga acgcattgct tatcaagcag
     1501 aatgcgattc tgatttctgc atcatccgct atcccgacca aaccgaccgc tttatccgcg
     1561 cggtgctgcc gattccgatt atcgggcatg aggaaacgct ggaatatggc gtgtgggtat
     1621 ccgtcagcga aaagagtttt aacgactatc aaagccgctt ttacgacaac cttgaaaacg
     1681 ctgtttattt cggcatgatc tgcaaccagc tgccccctta tgaaaccgac accttcggtc
     1741 tgcactgcaa tgtcgttacc caacccgatg gtcaacgtcc tttgttgcaa ctccatcaag
     1801 gcggcgaaca tccgctggta cgcgactttt atcacggcat agaatatgcc gaagcgcagg
     1861 caaggatagg ggcggtatcc gaatcggatt gctcaggata aaaggacaaa aaggatgaat
     1921 atgaaataca aagacctgcg cgacttcatc gccatgctcg agcagcaggg caaactcaag
     1981 cgcgtcgcac accccgtttc cccgcatttg gaaatgaccg aaatcgccga ccgcgtgttg
     2041 cgtgccgaag ggccggcgtt gctgtttgaa aacccgatta agcccgacgg tacgcgctat
     2101 gattatcccg tgttggcaaa cctgttcggc acgcccgaac gtgtggcgat gggcatgggc
     2161 gcggacagcg tgtccaagct gcgcgaaatc gggcagacgc tggcgtattt gaaagaaccc
     2221 gaaccgccca aaggcatcaa agacgcgttt tccaaactgc cgctgctgaa agacatttgg
     2281 agcatggcgc cgaacgtggt gaaaaacgcg ccgtgtcagg aaatcgtgtg ggaaggcgaa
     2341 aacgttgatt tgtataaact tccgattcag cattgctggc cggaagacgt tgcgccgctg
     2401 gtaacatggg gcttgaccgt aacgcgcggg ccgcacaaaa aacgccaaaa tctcggcatt
     2461 taccgccaac aattaatcgg caaaaacaag ctgattatgc gctggctgtc gcatcgcggc
     2521 ggcgcgttgg attatcagga attccgcaaa ctcaatcccg atacgccgta tcccgtcgcc
     2581 gtcgtactcg gctgcgaccc cgccaccatt ttgggcgcgg taacgcctgt tcccgatacc
     2641 ttgagcgaat accagtttgc cggactgctg cgcggttcgc ggacggagct ggtgaaatgt
     2701 atcggcaacg atttgcaagt gcctgcccgc gcagaaatcg tgttggaagg cgtcatccat
     2761 ccgaacgaaa ccgcgttgga aggcccgtac ggcgaccaca ccggctatta caacgagcag
     2821 gatcatttcc ctgtgtttac ggtcgaacgc atcaccatgc gcgaaaaccc gatttaccat
     2881 tcgacctaca cgggcaaacc gcccgatgag cctgccgttt tgggtgtggc attgaacgaa
     2941 gtgttcgtac cgcttttgca aaagcagttc cccgaaatca ccgatttcta cctgccgccc
     3001 gaaggctgtt cctaccgcat ggcggtgatc ggcatgaaaa aacagtacgc cggacacgcc
     3061 aagcgcgtga tgatgggctg ctggtcgttc ctgcgccagt ttatgtatac caaattcatt
     3121 atcgtggtgg atgacgatgt gaacgtgcgc gactggaaag aagtcatctg ggcggtcacc
     3181 acgcgcatgg accccgtgcg cgacactgtt ttggtagaaa acacgcccat cgactacctc
     3241 gacttcgcca gccccgtcag cggactcggc ggcaaaatgg gtttggatgc aaccaataag
     3301 tggccgggcg aaaccgaacg cgaatggggg cgtgtaatca aaaaagaccc tgccgttacg
     3361 gctaagattg atgggatttg gggggaattg gggttgtagg ttttacggta atgccgtctg
     3421 aagcttaatg tgtttcagac ggcatttttg tacgcagggt gtttacggtt ctttaggttt
     3481 gagccactgc cattgttcca gcagggtgtc ttcgaggatt ttctgtttct ctttgcgcga
     3541 attgatgccg ccggtcagcc tgccgtaatc gacggtaatg gacggtttgt ccactgtacc
     3601 ggtgattttc aggggaatcg gtttgttttt cggatggacg gcgttgcgga taaggacatc
     3661 ttcagacaat tcctgcgtat ccagattggt atagccgttg ctggtaacat agaggctgtc
     3721 ggagaagagt tcggtatcga tgtggcggct gatgccgtct gaaatttcgc tgttgagcgt
     3781 gaatcggtgg aagggtgtgc tgggtgcggc attgtcggca gttttgcccg aaatgccgtt
     3841 tttcaggata ttgtccatgt cgataccgtg ccacgcaccg ttggaaatat ttagcgacag
     3901 gctgccctga agcgagcgga taagctcttt tcgggtttcg ccgcccgcgg tcaggtcgat
     3961 gaccgcgtcg ccgttgccgc tgaagctgtg gaagccgaac aggtcttgca gcagcggttg
     4021 gatttggatg ttgcttgcat tctgttgcag gcggtaagtg gcgggacggg tgttggcgat
     4081 gctgatgccg ccttcggtat ggccgccgta aagccctgac ttgaaacggc tgagcgcgat
     4141 atggtctttg tcggcgtgga ggtaggtttc catatcgtcc agttgcaggc cgggaagttg
     4201 gacttttccg attttcaggt gcgcctcgat gtcgccggac agcttggcga gggtgtcggg
     4261 aaatattttg ccgttttgtt gccgcacgtc gtcaagatag ggggtcaggt tcaatttttg
     4321 cagtgcgacg gcggcttcca gatgcggtgc gtcttcatgt gtgtatctga attttgcggc
     4381 aacgggctgg cggtcgaatg tgccgtttaa ttcggcattc caattttgca gattcagtat
     4441 ggacatcgaa ccgtcgagcc ggctgatgaa acggggttgc ggcaggcggt tgacggtatc
     4501 ctgaagggtc gatatatgca ggcgcggtac ttccagcccg ttgtcccggc tccagaccaa
     4561 cggcgagccg agggaaaaat tggtttgaag gcggggtgtt ttgacgttgc cggagatttc
     4621 ggcgttgccg atgttgccga tgccgggatg gaggtcggcc ctatcgagtt tgaacgatcc
     4681 gccccactgg gaattttcgc cggcagcggt aaatgtcccg ttggcggttt caattttgat
     4741 gccgttgttc ctgagtgcca gcgcagggat ttgggcggtc aggtggaggt tgtggaaggc
     4801 ggtgtcggca cgcagggcga ggtcggcggc atccgccccg ccggcgttga agcggatcgt
     4861 ggggctgccg gtggtggaaa cggcaatgcg gtgtccgttc agggcggtct cggcttcgaa
     4921 atggaacggt gagattttgg gcgtgccgat gccgtctgaa aggaacagcc ccctgctttt
     4981 ccacgggacg gacagcttcc cccaaaccag tatgcccgaa ctttcaaact gctgccccgc
     5041 cgaatcgggg gattgcaggt tgaggctgac ttccttcagg gtaagccggt cttgcggcaa
     5101 gtcgaggcgg acggtactgt tttcgatgat gatgcggttt accgaggctt tgcgttttgg
     5161 gttgtcgatc aggtcttgga tattccaaac gcctttcctg tcgcgcgtca gggcaagctc
     5221 cgcgcccgaa accacccatt tttcaatctg tatctgatcc gaccacagat ttttccagcc
     5281 caatccgatt ttggtttctt tgacggaaac ggcagtccgg ttgctgccgg gttcggtaat
     5341 ggtcaggttt ttcaggatga cggtcggtcg gggcagaagc ctgcgcccta tatccgcatc
     5401 aaacgagatt ttccggtgcg tatgggcaat gctttgttgg aggcggcttc ggatgttttc
     5461 aggcgtgaag atgcgataga cggacgcgtg cagcccgatt gccgccaaaa gcagcatcgt
     5521 cagcaccgct accgcatatt tcagacggta tttgtggaag accgataata aatccatagc
     5581 ccgaacaatc cgtaaaacac gccgcagtat aacacggctt gcggcaatcc tattttatgc
     5641 gggcggatta tagtggatta acaaaaacc
//
LOCUS       DAWKOD010000130         5659 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000130 DAWKOD010000000
VERSION     DAWKOD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.130"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            97..813
                     /locus_tag="WJK73_001651"
     CDS             97..813
                     /locus_tag="WJK73_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ7558103.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WJK73_001652"
     CDS             962..1864
                     /locus_tag="WJK73_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ7558104.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WJK73_001653"
     CDS             1907..2680
                     /locus_tag="WJK73_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ7558105.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WJK73_001654"
     CDS             2792..4480
                     /locus_tag="WJK73_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ7558106.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WJK73_001655"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WJK73_001655"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ7558107.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1308 a         1542 c         1567 g         1242 t
ORIGIN      
        1 atttgtgcaa tgctttaatt tccccgacac gatttgaaac acagccgcct taaaacatct
       61 taagaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc tggcaaaaag
      241 ctgaccgaat ccgaactttg ccgccagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgatac ggcgcgaaga agaggcctcc ggcaggggag accgcgttgg ctggaaccgc
      541 ctgtccaatg cctttcacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggagcgtt tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacgt tgctttcggt attgataccg atgtttgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggataaggtg ctatcggtct tggtgtatgc
     1081 tgtgctgctg ctgatcggcg tctcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggtgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcatccgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaaacgcg ggtatgtatt gtctgatgct
     1381 gctggtgctc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcaggcgg
     1501 gctgctgttt gccgcatcgg cagacggtgt gtcgtgggtg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgaattgga
     2041 ggatgctggc agcctgaaac gccaccacgg cggcgcatcc tcgggcggaa acttgccgga
     2101 ggggctgcct gccagccgcc aaattcaatg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa catattcccg acgagtcatc gctgtttgtc agtatcggca cgacgatgga
     2221 agccgtggca tcggagctgg tgagacggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gcattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaacgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccatca tcgaagacga cctcaaaagc ggcaaacacg aagccgtcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagatg tcgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttgatta aagacggcaa agcgtatgtc gatgatttga cgcccgaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcgggtaaa aacagccctt accgcgaccg
     3241 cagcgtcgaa gaaaacctcg acctgttcac acgcatgaaa aacggcgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatcgggc aacatcaata tgcgcgaccc
     3361 cgtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgtatcta
     3421 cccgatgtac gactacacgc attgcatctc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cacaccgtcc gctgtacgac tgggtgttgg ataacatcca
     3541 cgcgccgcat gccacccgtc cgcgccaata cgagttttcc cgtttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacta tttccggtat gcgccgacgc ggctacacgc ccgaaggcct
     3721 gcgtctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaagccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccatcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggttggcg gctgtttcat
     4681 accttgagcg gggaattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac agtcgatgcg tgacttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca ttatcatcaa accgctggac ggtatgggcg gtatgggtat tttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg acgcggcgtg gcgcaggaat tggacggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ttgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag ccggcaggga tgcggttaat gccgtacgga caccccaccg
     5641 ccccgttttc agacggtat
//
LOCUS       DAWKOD010000131         5645 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000131 DAWKOD010000000
VERSION     DAWKOD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.131"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..889)
                     /locus_tag="WJK73_001656"
     CDS             complement(128..889)
                     /locus_tag="WJK73_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558108.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDIRKILNDIKYELKYKR"
     gene            989..1240
                     /locus_tag="WJK73_001657"
     CDS             989..1240
                     /locus_tag="WJK73_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558109.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1292..2098
                     /locus_tag="WJK73_001658"
     CDS             1292..2098
                     /locus_tag="WJK73_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ7558110.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2405..3928
                     /locus_tag="WJK73_001659"
     CDS             2405..3928
                     /locus_tag="WJK73_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ7558111.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4026..5438
                     /gene="trkA"
                     /locus_tag="WJK73_001660"
     CDS             4026..5438
                     /gene="trkA"
                     /locus_tag="WJK73_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ7558112.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1404 a         1702 c         1371 g         1168 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat attaatattt tgcgcctgtt
       61 ccatgaggct ttcaagtcgg acgggcagga aaatgccgtc tgaaacggct ttcagacggc
      121 atggcaatca gcgtttgtat tttaactcgt acttgatgtc gttgaggatt ttgcggatat
      181 cgtgttccaa cacgtcttcg actaccgcac ccgcctgctc gtgcagcatc tgctggagct
      241 ggtaggtaaa aaccgccatc tgcttttgca ccgccgtacg gatgatgccg ttgacggtat
      301 cggtcagatg cgggcgcagg cgtttgatca gccgctcggt cagctcctgt tcggacaggc
      361 agaacacttc gcgccggttg acggctttcg ggttcaggat attgatttgg acgggcatca
      421 acgcttcttc cgcaccgcct tcgccgtttt ccgcaacccc gtctgccgcc gtacccgatt
      481 ctgccgcatc ggcgttttcc ccgccttcaa tctgtccggt ttcaaattcg gcactgtctt
      541 ttttggcatc aaaccggatt ctccgccgcg attcgatgtg tttttccgaa accgacattt
      601 gcagggaagc ctgcgcgttg agccagtttt cctgaaggac gatcatcggg tcggtttcga
      661 tttcttcccc gcaatcggca acggcggcat tgtgctcctc ctgccatttc ttcagatacg
      721 cctttaacac acggtttcgg cttgcatcgt ccagtttcgg cacagacgcg tccgttccgg
      781 tttcagaagg gcgggacagc ggcgcgtaag tcggcactgc cttcatacgg cgcgtctgac
      841 gcaggttttc caaacgtttt tcccaattcg gctctttatt cgcatccatt ttcggcttcc
      901 ggttcttaat ctttgcaggc agacaaaccc gcgcccaaag cgcggtttga tataatggcg
      961 cattttaaca gattcgcgag gatacatcat gggcagcatc gaacagcgtt tggaatatct
     1021 ggaagaggcg aacgacgtgc tgcgtatgca gaaccacgtc ctgtccaccg cattcaaagc
     1081 cttaatccgc gcccttcccg ccgaaaccgc cgaaatcgcg gtcgagtcga ttcagcttgc
     1141 ttttgaggac gccttggcag aattgagcta tgaggacagc ccccataccg atttgttcca
     1201 cgacgttact tatgcgtttt tccgtgaaaa agaacgttaa ttttgtgtta aactgatttt
     1261 ttaggctttt tgattaccga aaggaatttt gatgaatatg aaaaaatgga ttgccgccgc
     1321 ccttgcctgt tccgcactcg cgctgtctgc ctgcggcggt cagggcaaag atgccgccgc
     1381 gcccgccgca aaccccgaca aagtgtaccg cgtggcttcc aacgccgagt ttgccccctt
     1441 tgaatcttta gactcgaaag gcaatgtcga aggtttcgat gtggatttga tgaacgcgat
     1501 ggcgaaggcg ggcaatttta aaatcgaatt caaacaccag ccgtgggaca gccttttccc
     1561 cgccttgaac aacggcgatg cggacgttgt gatgtcgggc gtaaccatta ccgacgaccg
     1621 caaacagtct atggacttca gcgacccgta ttttgaaatc acccaagtcg tcctcgttcc
     1681 gaaaggcaaa aaaatatctt cttccgacga tttgaaaaac atgaacaaag tcggcgtggt
     1741 aaccggctac acgggcgatt tctccgtatc caaactcttg ggcaacgaca acccgaaaat
     1801 cgcgcgcttt gaaaacgttc ccctgattat caaagaactg gaaaacggcg gcttggattc
     1861 cgtagtcagc gacagcgcgg tcatcgccaa ttatgtgaaa aacaatccga ccaaagggat
     1921 ggacttcgtt accctgcccg acttcaccac cgaacactac ggcatcgcgg tacgcaaagg
     1981 cgacgaagca accgtcaaaa tgctgaacga tgcgttggaa aaagtacgcg aaagcggcga
     2041 atacgacaag atttacgcca aatattttgc aaaagaagac ggacaggccg caaaataagc
     2101 ccgcccgtcc gaacacaatg ccgtctgaag ccctttcaga cggcattgtt catcaatcgg
     2161 cctacaatga actgcctgct gatttctccc taccgcaaag caacaggcaa agattacaaa
     2221 tatcaaaatc cgagtaaaac agtattttat taaaacaaat tgataatcaa gagattagaa
     2281 ttatgtattg tctttaccgt acaaacgctg gcactatttc aacctgataa aaaacagcct
     2341 tcaaaaaggt tgcttaaaac agcagcagac acttaccgcc acaaccttga aaaggaacac
     2401 aatcatgacc gtcatcaagc aagaagactt tatccaaagt atctgcgatg ccttccaatt
     2461 catcagctac taccatccga aagactacat cgacgcgctt tataaggcgt ggcagaagga
     2521 agaaaatccc gccgccaaag acgcgatgac gcagattttg gtcaacagcc gtatgtgtgc
     2581 ggaaaacaac cgccccatct gccaagatac cggtatcgca accgtgtttt tgaaagtcgg
     2641 tatggatgtg cagtgggatg cggatatgag cgtggaagaa atggttaacg aaggcgtgcg
     2701 ccgcgcctac acttgggaag gcaatacgct gcgcgcttcc gttctcgccg accccgccgg
     2761 caaacgccaa aataccaaag acaacacgcc cgccgtcatc catatgagca tcgtgccggg
     2821 cgacaaagtc gaagtaacct gcgcggcaaa aggcggcggc tctgaaaaca aatccaaact
     2881 cgccatgctc aatccttccg acaacatcgt cgattgggta ttgaaaacca tcccgaccat
     2941 gggcgcgggc tggtgtcctc ccggcatctt gggcatcggc atcggcggca cgcccgaaaa
     3001 agccgtgttg atggcaaaag aatccttgat gagccacatc gacattcaag agttgcagga
     3061 aaaagcagcg tcaggtgcag aattgtccac caccgaagcc ctgcgcctcg aactctttga
     3121 aaaagtcaac gcgctaggca tcggcgcgca aggcttgggc ggtctgacca ccgtgttgga
     3181 cgtgaaaatc ctcgattacc cgacccacgc cgcctccaaa ccgattgcca tgattccgaa
     3241 ctgcgccgcc acccgccacg tcgaatttga attggacggc tcaggccctg tcgaactcac
     3301 gccgccgcgc gtcgaagact ggcccgattt gacttacagc cccgacaacg gcaaacgcgt
     3361 cgatgtcgac aagctgacca aagaagaagt ggcaagctgg aaaaccggcg acgtattgct
     3421 gttgaacggc aaaatcctca ccggccgcga tgccgcacac aaacgcctcg tcgatatgct
     3481 caacaaaggc gaagaattgc ccgtcgattt caccaaccgc ctgatttact acgtcggccc
     3541 cgtcgatccg gtcggcgatg aagtcgtcgg tccggcaggt ccgaccacag ccacccgcat
     3601 ggacaaattc acccgccaaa tgctcgaaca aaccgacctc ttgggcatga tcggcaaatc
     3661 cgagcgcggc gtggccacct gcgaagccat cgccgacaac aaagccgtgt acctcatggc
     3721 agtcggcggc gcggcgtatc tcgtggcaaa agccatcaaa tcttccaaag tcttggcgtt
     3781 ccccgaattg ggcatggaag ccatttacga atttgaagtc aaagacatgc ccgtaaccgt
     3841 cgccgtagac agcaaaggcg aatccatcca cgccaccgcc ccgccccaat ggcaggcgaa
     3901 aatcggcatc atccccgtca aatcttgagg cgtcatgccg tctgaacaca aaatctgctt
     3961 tcagacggca tttccgcccc tggttgcggt acaatccacc atttcatcac tcggcgaccc
     4021 acaccgtgaa aatcctcatt ttaggcaacg gacaggtagg ttctaccgtc gcacaaaacc
     4081 ttgccgccat acccaacaac gacgtaaccg ttatcgacat cgacgaaaaa gcattgcagg
     4141 aaacaggcag ccgcctcgat gtccaaaccg ttttcggcaa cggcgcatcc cccttcacat
     4201 tagaacgcgc cggcgcggaa gatgccgact tgctgctcgc gctctcccgc agcgacgaaa
     4261 ccaacatcgt cgcctgcaaa gttgccgccg acctgttcaa catccccggc cgcatcgcgc
     4321 gcgtccgttc cagcgaatac ctcgaatacc tcagccccaa gctcgaaaac aacgaaaacg
     4381 gcagcctttc catattcggc ataaccgaaa ccatcagccc cgaacagctc gttaccgaac
     4441 agcttgccgg cctgatagac tgcccgggcg cattgcaggt tttacgtttt gcagacgacc
     4501 gcgtgcggat ggtcatcata caggcgcggc gcggcggact gcttgtcgga cgcagcattg
     4561 ccgacatcgc ccaagatttg cccgacgggg ccgactgcca aatctgcgcc gtttaccgca
     4621 acaaccgcct catcgtcccc gcgccgcaaa ccgtcatcat cgaaggcgac gaaatcctat
     4681 ttgccgccgc cgccgaaaac atcggcgcgg tcatacccga attgcgcccc aaagaaacca
     4741 gcacccgccg catcatgatt gccggcggcg gcaacatcgg ctaccgtctc gccaagcagc
     4801 tcgaacacgc atacaacgtc aaaatcatcg aatgccggcc gcgccgtgcc gaatggatag
     4861 ccgaaaacct cgacaacacc ctcgtcctgc aaggttcggc aaccgacgaa accctgctcg
     4921 acaacgaata catcgacgaa atcgacgtat tctgcgccct gaccaacgac gacgaaagca
     4981 acattatgtc cgcccttttg gcgaaaaacc tcggcgcgaa gcgcgtcatc ggcatcgtca
     5041 accgctcaag ctacgtcgat ttgctcgaag gcaacaaaat cgacatcgtc gtctcccccc
     5101 acctcatcac catcggctcg atactcgccc acatccggcg cggcgacatc gttgccgtcc
     5161 accccatccg gcgcggcacg gcggaagcca tcgaagtcgt cgcacacggc gacaaaaaaa
     5221 cttccgccat catcggcagg cgcatcagcg gcatcaaatg gcccgaaggc tgccacattg
     5281 ccgccgtcgt ccgcgccgga accggcgaaa ccattatggg acaccatacc gaaaccgtca
     5341 tccaagacgg cgaccacatc atctttttcg tctcgcgccg gcgcatcctg aacgaactgg
     5401 aaaaactcat ccaagtcaaa atgggctttt tcggataaac cgccccattc cggacacatt
     5461 gccgccaagc ggtatggaag cggaataatg gtaggtgggc ttcagacggc atcgtccccc
     5521 accgttattc ccgcgcaggc gggaatccgg tctgttcggt ttcagttgtt tttgggtttc
     5581 gggtaatttc caaatcgtca ttcccgcaca ggcgggaatc caaaccattg gacagcggca
     5641 atatt
//
LOCUS       DAWKOD010000132         5603 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000132 DAWKOD010000000
VERSION     DAWKOD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.132"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WJK73_001661"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WJK73_001661"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ7558113.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYSEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            1137..3002
                     /gene="msbA"
                     /locus_tag="WJK73_001662"
     CDS             1137..3002
                     /gene="msbA"
                     /locus_tag="WJK73_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ7558114.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3102..4667
                     /gene="guaA"
                     /locus_tag="WJK73_001663"
     CDS             3102..4667
                     /gene="guaA"
                     /locus_tag="WJK73_001663"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ7558115.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4729..5475
                     /gene="fabG"
                     /locus_tag="WJK73_001664"
     CDS             4729..5475
                     /gene="fabG"
                     /locus_tag="WJK73_001664"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ7558116.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1370 a         1796 c         1364 g         1073 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggacaaa
      241 ccgtcaatac ccagcccatc atgctcgccg ccggcgttgc cgtttaccgc gcctattcgg
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg gattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtctgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgaaca ggtaaaacaa atttgcgccg aatccacaca aggcgaagtg gttgaagccg
      541 tcaacttcaa ctcgcccgga caagtcgtga ttgcaggcaa taccgccgcc gtcgagcgcg
      601 ctatgcttgc cgccaaagaa gccggcgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact cgccgaagcc ctgaaaaccg
      721 ttgaaatcaa gcagccgcaa atccgcgtta tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgccctcgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaagccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaaa cgcactgact tcgttccgca aaaagcagcc
     1021 tgcatgcctc aggctgcttt tcacgtccga acggcaaaca gccgcatatt tgcgctataa
     1081 ttgatgacga ccaaaccacc gacagcggct gccgttgcag ttcccgcccc accgacatga
     1141 tagaaaaact gactttcgga ctgtttaaaa aagaagacgc gcgcagcttt atgcgcctga
     1201 tggcgtacgt ccgcccctac aaaatccgca tcgttgccgc cctgattgcc attttcggcg
     1261 ttgccgccac cgaaagctac cttgccgcct tcatcgcccc cctgattaac cacggctttt
     1321 ccgcacctgc cgcgccgccc gagctgtctg ccgccgccgg catcatttcc accctgcaaa
     1381 actggcgcga acagtttacc tatatggttt gggggacgga aaacaaaatc tggaccgtcc
     1441 cgctctttct catcattctc gtcgtcatcc gtggcatctg ccgctttacc agcacctatc
     1501 tgatgacttg ggtctccgtg atgaccatca gcaaaatccg caaagatatg tttgccaaaa
     1561 tgctgaccct ttcctcccgc taccatcagg aaacgccgtc cggcaccgta ctgatgaata
     1621 tgctcaacct gaccgaacag tcggtcagca acgccagcga catcttcacc gtcctcacgc
     1681 gcgacacgat gattgttacc ggcctgacca tcgtcctgct ttacctcaac tggcagctca
     1741 gccttatcgt cgtcctgatg ttccccctgc tctccctgct ctcgcgctac taccgcgacc
     1801 gtctgaaaca cgtcatttcc gactcgcaaa aaagcatagg cacgatgaac aacgtgattg
     1861 ccgaaaccca tcagggacac cgcgtcgtca agctgttcaa cgggcaggcg caggcggcaa
     1921 accggttcga cgcgatcaac cgcaccatcg tccgcctcag caaaaaaatc acgcaggcaa
     1981 cggcggcaca ttccccgttc agcgaactaa tcgcctcgat cgccctcgcc gtcgtcatct
     2041 tcatcgccct gtggcaaagc caaaacggct acaccaccat cggcgaattt atggcattca
     2101 tcgtcgcgat gctgcaaatg tacgccccca tcaaaagcct tgccaacatt agcatcccta
     2161 tgcagacgat gttcctcgcc gccgacggcg tatgtgcatt tctcgacacc ccgcccgaac
     2221 aggacaaagg cacgctcgca ccgcagcgtg tcgaagggcg catcagcttc cgcaacgtcg
     2281 atgtcgaata ccgttcggac ggcatcaaag ccctcgacgg cttcaacctc gacatcagac
     2341 aaggcgaacg cgtcgccctg gtcggacgtt ccggcagcgg caaatccacc gtcgtcaacc
     2401 tgctgccccg ctttgtcgaa ccgtctgccg gcaacatctg catagacggt atcgacatcg
     2461 ccgacatcaa actcgactgc ctgcgcgccc aatttgccct cgtctcccaa gacgtattcc
     2521 tgtttgacga caccctgttt gaaaacgtcc gatatagccg tcccgacgcg ggcgaagccg
     2581 aagtcctatc cgccctccaa gccgctaacc tgcaaagcct gatcgacgca tcaccgctcg
     2641 gactgcacca gcccatcgga tcgaacggca gcaacttatc cggcggacag cggcaacgcg
     2701 ttgccattgc ccgcgccatt ttgaaagacg cgccgatatt attattggac gaagccacca
     2761 gcgcattgga caacgaatcc gaacgcctcg tccaacaggc gctcgaacgc ctgatggaaa
     2821 accgcaccgg catcatcgtc gcccaccgcc tgaccaccat cgaaggggcc gaccgcatca
     2881 tcgtaatgga cgacggcaaa atcatcgaac aaggcacaca cgaacaactg atgtcccaaa
     2941 acggctacta cacgatgtta cgcaatatct caaacaaaga tgccgccgtc cggacggcat
     3001 aaacaaaatg ccgtctgaaa tggtacaatc gccccgaccc tttcagacgg catcatatcc
     3061 gccgacccat ccgattatct tcaatcactg taaaacccat tatgacccaa gacaaaatcc
     3121 tcatccttga cttcggttcg caagttaccc agctcatcgc ccgccgcgtg cgcgaagccc
     3181 acgtttactg cgagctgcat tccttcgata tgcctttgga cgaaatcaaa gccttcaacc
     3241 ccaaaggcat catcctttcc ggcggcccta attccgttta cgaatccgac tatcaagccg
     3301 ataccggtat ctttgacttg ggcattccga ttttgggcat ttgctacggc atgcaattta
     3361 tggcgcacca cttgggtggt gaagtgcaac ccggcaacca gcgcgaattc ggttacgcgc
     3421 aagtcaaaac catcgacagc gagctgacac gcggcattca agacgatacg ccaaacacgc
     3481 tcgacgtatg gatgagccac ggcgataaag tttccaaact gcctaccggt ttcaccgtca
     3541 tcggcgacac gccgtcctgc ccgattgcca tgatggaaaa cgccgaaaaa caattctacg
     3601 gcatccaatt ccaccccgaa gtaacccaca ccaaacaagg ccgcgccctg ttgaaccgct
     3661 ttgttttgga tatttgcggc gcacaaccgg gctggacaat gccgaactac atcgaagaag
     3721 ccgttgccaa aatccgcgaa caagtcggca gcgacgaagt gattttaggc ttgtccggcg
     3781 gcgtggactc ttccgtagcc gccgcgctga tccaccgcgc catcggcgac caactgacct
     3841 gcgtgttcgt cgatcacggt ttgttgcgcc tgaacgaagg caaaatggtg atggatatgt
     3901 tcgcccgcaa cttgggtgtg aaagtgattc acgtcgatgc cgaagggcag tttatggcga
     3961 aactcgccgg cgttaccgac cctgagaaaa aacgcaaaat catcggcgcg gaatttatcg
     4021 aagtatttga tgccgaagag aaaaaactca ccaacgccaa atggctggcg caaggcacga
     4081 tttaccccga cgtaatcgaa tccgcaggcg cgaaaaccaa aaaagcccac gccatcaaat
     4141 cccaccacaa tgtcggcggc ctgcctgaaa acatgaagct caagctgctt gagccgttgc
     4201 gcgacttgtt taaagacgaa gtgcgcgaac tgggcgttgc tttgggtctg ccgcgcgaaa
     4261 tggtgtaccg ccatccgttc cccggccccg gtttgggcgt gcgcatcttg ggcgaagtga
     4321 aaaaagaata cgccgactta ttgcgtcaag cagacgatat tttcatccaa gaattacgca
     4381 acaccaccga cgaaaacggc acatcttggt acgacctgac cagccaggca tttgccgtat
     4441 tcctgcctgt gaaatccgtc ggcgtaatgg gcgacggacg cacttacgac tacgtcgtcg
     4501 cactgcgcgc agtcatcacc agcgacttca tgaccgcaca ctgggcagaa ctgccttact
     4561 cactgctcgg acgcgtgtcc aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg
     4621 tgtacgatgt cagcggcaaa ccgcctgcca ccatcgagtg ggaataacca gcaaacaggg
     4681 ctgcaccgtc cggcgcagtc tttcgattat cggaaaaaag gaaaaaatat gagcacacaa
     4741 gatttaagcg gcaaaatcgc tttggtaacc ggcgcatcgc gcggtatcgg tgcagcaatt
     4801 gccgacacac tggcggcagc cggtgccaaa gtcatcggta cggcgaccag tgagagcggt
     4861 gcggcggcga ttagcgagcg gttggcgcaa tggggcggcg aaggccgcgt attaaattcc
     4921 gccgaacccg aaaccgtcga aaacctgatt gccgacatcg aaaaaacgtt cggcaaactc
     4981 gatattctgg tcaacaacgc cggcatcacc cgcgacaacc tcctgatgcg catgaaagaa
     5041 gaagagtggg acgacatcat gcaggtcaac ctcaaatccg tgttccgcgc ttctaaagcc
     5101 gttttgcgcg gtatgatgaa acaacgttcc ggccgcatca tcaacatcac atccgtcgtc
     5161 ggcgtgatgg gcaatgccgg tcaaaccaac tatgccgcgg caaaagcagg cttaatcggt
     5221 ttctccaaat ccatggtgcg cgaagtcggc agccggggca ttaccgtcaa ctgcgtcgcc
     5281 cctggcttta tcgataccga catgacacgc gccctgccgg aagaaacccg ccaaaccttt
     5341 accgcccaaa ccgccttggg cagattcggc gacgcgcaag acatcgccga tgcggttctg
     5401 ttcctcgctt ccgaccaagc aaaatacatc accggccaaa cgctgcacgt caacggcggt
     5461 atgctgatgc cctgacgggc agccgtaccg acaatgccgt ctgaagccct ttcagacggc
     5521 atttgcattc tcaggcaaaa tgaacacacg ccacaccccg ccctgcccct gcggatcagg
     5581 cacaagctga gacctttgca aaa
//
LOCUS       DAWKOD010000133         5538 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000133 DAWKOD010000000
VERSION     DAWKOD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.133"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..77)
                     /locus_tag="WJK73_001665"
     CDS             complement(<1..77)
                     /locus_tag="WJK73_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7558117.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTART"
     gene            complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJK73_001666"
     CDS             complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJK73_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ7558118.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJK73_001667"
     CDS             complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJK73_001667"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ7558119.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISVALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITHRIAGYASPIDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4657..5478
                     /locus_tag="WJK73_001668"
     CDS             4657..5478
                     /locus_tag="WJK73_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ7558120.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1178 a         1301 c         1606 g         1453 t
ORIGIN      
        1 gttcttgcag ttacacctgc gacaaacagt tcaatgagtt tattttgttt atactggctt
       61 agacgacttt ttctcatagg ggcaactcta acttaatttg aatttcccta gttatctagg
      121 acagccccaa atttatacaa aaatgagcgc ggttcggcgc aaccttgaat caagttcccg
      181 catcggtttt cattgccggt acggatgcgt tcaagccggt tttgcaaagg ccgcgctttc
      241 ggcaagcgga cacggacact gccgacggtt gcgccgttaa cggggggagg gaggagctgc
      301 gccgaccgtg tgaatgaaag tgccgtctga aacccgattt tcaggcttca gacggcattt
      361 cgcattaatg cgggcggcgc gtttatttgc cgcgcatcag ttcaaagaaa tcgtcgttgt
      421 ttttagaggc tttgattttc ccgtttaaga actcggtagc ttcgatttca tccatcgggt
      481 gcaggaactt gcgtaagagc cacatacgtt gcaactggtc gttcggcaca agcagctctt
      541 cgcggcgcgt gccggatttg ttgatgttga tggcggggaa gaggcgtttt tccgccatac
      601 ggcgatcaag gtgcaattcc atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt
      661 ccatacggct gccggtttca accaatgcgg tggcgataat ggtcagcgaa ccgccttctt
      721 ccacgttgcg cgccgcaccg aagaaacgtt tggggcggtg cagcgcgttg gcatcgacac
      781 cgccggtcag gattttgccc gaggtgggga cgacggtatt gtaggcgcgg gcaaggcggg
      841 taatcgaatc cagcaggatg accacgtctt ttttgtgttc caccatacgc ttggcttttt
      901 caagcaccat ttcggcaact tggacatggc gttgagccgg ctcgtcaaag gtggaggaaa
      961 ccacttcgcc acggacggag cggctcattt cggttacttc ttcgggacgt tcgtcaatca
     1021 agaggacgat gagttcgact tcgggatagt ttgcggtaac ggcgtgggca atgttttgca
     1081 gcatcacggt tttaccgctt ttgggcgggg caaccaagag ggcgcgctga cctttgccga
     1141 tgggggaaat caggtcgatg gcgcgtccgg tcaggttttc ttcggacttt aagtcgcgtt
     1201 ccagcttcaa ctgttcggtc ggaaacagcg gggtcaggtt ttcaaacagg attttatggc
     1261 ggcatacttc cgggtggtcg ccgttgatgg tatcaagcct gaccagggca aaatagcgtt
     1321 cgttgtcttt tgggacgcgg acgctgcctt cgatggtgtc gcccgtatgc aggttgaagc
     1381 ggcggatttg ggtgggcgag acatagatgt cgtcggggcc ggcaagatag gacgtgtccg
     1441 cgctgcggag gaagccgaag ccgtcgggca ggatttcaag cgtgccggag caggtgaaac
     1501 cctcgccttt tttcatcatc tggcggacga tggcaaatac gaggtcttgt ttgcggaatc
     1561 ggttggcgtt ttcgatgccg tgttcttccg ccaattctaa gagtttggaa atgtgcaggg
     1621 tttgtaattc ggagacgtgc ataataatga tgtattttga agaggaaaaa gacaggcaga
     1681 tgccgtctga aagaagaagc tgaccgttgc cggttgctcg gggaaggggg aattgtaggc
     1741 agtcggcgcg tgggtgtcaa atattatcgc ggacggggca tcggcaggaa atgccgtctg
     1801 agcggagctg cttggaaaaa aatacccccg cgcttttcag gctcgggggt atgggcattg
     1861 attatttgtt caattcattc gccaaatata gccaagtttc gatgacggta tccgggttca
     1921 gggaaacgct ttcaatgcct tcctcaacca gccatttggc gaagtccgga tggtcggacg
     1981 ggccttgacc gcagatgccg acatatttgt tctgcttgcg gcaggcggag atggcaagat
     2041 gcagcatcac tttgacggca gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt
     2101 cgcggtcgag accgagggtc agttgggtca tgtcgttcga gccgatggag aagccgtcga
     2161 agtattgcag gaattgttcc gccaataccg cgttgctcgg cagctcgcac atcataatca
     2221 ggcgcaggcc gtttttgccg cgttccaagc cgttttcttt cagggctttg acaacggctt
     2281 cggcttcgcc caaagtgcgg acgaacggaa tcatgatttc aacgttggtc agacccattt
     2341 cgtcacgaac gcgtttcaag gctttgcatt cgagggcgaa gcagtctttg aaactttcgg
     2401 cgacataacg cgccgcaccc cggaagccca acatcgggtt ttcttcgtgc ggttcgtaga
     2461 tgctgccacc caccaagtta gcgtactcgt tggatttgaa gtcggacata cggacgatgg
     2521 ttttacgcgg ataaaccgat gcggctaaag ttgccacgcc ttctgcgatt ttatcgacat
     2581 agaagtcaat aggagaggcg tagccggcga tgcggtgggt aatttcagct tttaactcgt
     2641 cgtcttgttt gtcaaattcc aacaaggctt tggggtggat gccgatttgg cggttgatga
     2701 tgaattccat acgcgccaag ccgatgcctt cgctcggcag gttggcgaag ctgaatgcga
     2761 gttcgggatt gccgacgttc atcatcactt tgacaggtgc tttgggcata ttgtccaagg
     2821 cgacatcggt aatctgtacg tccaacaggc cggcatagat gaagccggta tcgccttcgg
     2881 cgcaggatac ggtaacttcc tgaccgtttt tcagcaattc ggttgcattg ccgcagccga
     2941 cgacggcagg aatgcccaat tcacgcgcga taatcgctgc gtggcaggta cgtccgccgc
     3001 gattggtaac gatggcggaa gcgcgtttca tcaccggttc ccaatccgga tcggtcatat
     3061 cggtaaccag tacgtcgccg gcttcgacgg aatccatctc ggaagcatct ttaatcaggc
     3121 gtactttgcc ctgtccgact ttctgaccga tggcgcggcc ttcgcacaag acggtttttt
     3181 cgccgttgat agaaaagcgg cgcaggttgc ggctgccttc ttcttgggat ttcacggttt
     3241 cgggacgggc ttgcaggata tagagtttgc cgtccaagcc gtcgcgaccc cattcgatat
     3301 ccatcgggcg gccgtagtgt ttttcgatgg tcagcgcgta atgcgccaac tcggtgattt
     3361 cttcgtcggt aatggagaag cggttgcggt cttcttcggg gacatcgacg ttggttaccg
     3421 atttgccggc ttcggctttg tcggtaaaaa tcattttgat gtgtttcgaa cccatggttt
     3481 tacgcaggat ggcgggcttg cccgctttga gcgtaggttt gaacacataa aattcgtccg
     3541 ggttgaccgc gccttgtacg acgttttcgc ccagaccgta agaggaggta acaaagacga
     3601 cttgatcgta gccggattcg gtgtcgaggg tgaacatcac acctgatgcg ccactgtcgg
     3661 aacgcaccat gcgttgaacg ccggcggaaa gggcgacgat gtcgtgttcg aagcctttgt
     3721 ggacgcggta ggaaatggcg cggtcgttgt acagggaggc gaatacatgg cgcatcgctt
     3781 ctttaacgtt ttccaagccg ttgatgttca agaaggtttc ctgttgtccg gcaaatgatg
     3841 cgtccggcag gtcttcggcg gttgcggaag agcgtacggc aacggaaatg tccgcaccgc
     3901 cggcatcggc aaccattttg ttccatgccg cttcgatttc ggcatcgagc tgttcgggga
     3961 agggcgtgtc caaaatccat tggcggattt ctttgccgac gcgtgccagt tcggcaacgt
     4021 cttcgacatc caattttgcc agtgcgacgg aaatgcgttc gctcagaccg ttgtgtgcga
     4081 ggaatgcgcg gtaggcatcg gccgtggtgg caaagccgcc ggggacgcga acgccttttt
     4141 cggtcagctg actaatcatt tcacccagcg aggcgttttt accgcccacg cgttcaacat
     4201 ctgtcatacg caggttttca aaccagatta cgtagttgtc ggccatttgt gtgtccaatc
     4261 caaaatatgt taaaaaagaa acaaatccgc gtgcttattt taagcgattc gttcctctgc
     4321 tgtcatgtgt tttatccgtt ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg
     4381 tgtgtagcgg tttgaaactt gcagccggta tacttctttt tttgggtatt ttctttgtaa
     4441 aacaggtggt ttgaataggt taatgttttt tctgtttgat ttttttgttt attttttaaa
     4501 attttctgcc aaaaaatact ttatataaat aattttattt caaaattata ttgtgtctgt
     4561 ttgggtgtaa tccgaggtag gcgtgctgcg ggtgctttcc ttgtgtctgc cgctgctgtt
     4621 atgatgatat ttttattctg tatttaagga tggaagatga gcagtccgcg ccatgtgttt
     4681 tacatttccg accgtaccgg tctgactgcc gagaatatcg gcgaggcttt gctgaaccag
     4741 tttggcaatc tgtcgttcaa acgccatacg catccgtttg tcgatacgcc ggaaaaggcg
     4801 cgcgcggtgg tggagaaggt caatcggagc cggcaggaaa acggtcagcg tccgattgcg
     4861 tttgtcagtg tggttgatga cgaaatccgt cggattatca aaggggcgga tgcttttcag
     4921 attaatttct ttgagacttt tttgggactg ttggagaaag aactcaatac cgaagccacg
     4981 gcatccgagc aggggcatca cagtatcggt aatacgaagc gttacgatgc gcgtatggag
     5041 gcggtaaatt tctcattgaa ccacgatgac ggggtcagcg ataagaacct tcaggaagcg
     5101 gatgtaatct tgatgggtgt atcgcgttcg ggcaaaacgc cgacctgcct ttacctcgcc
     5161 ctgcaatacg gcatccgtgc ggcaaactat ccgctgattc ctgacgattt ggaatcggcc
     5221 gatctgccgc gtatggtcaa gccttatagg gataagctgt tcgggttgac catccagccg
     5281 gaacgtttgc aggccatccg ccaagagcgc cgcccgaatt caacttatgc caaaatcgat
     5341 acatgccgca gcgaggtggc agacgcgcag agtatgttca gacggcatgg gattccgttt
     5401 gcgaatacga cggataaatc agtggaagag cttgccgtac acatccttca ggcgtgcaag
     5461 ctcaaacgca ggttttgacg ggttttgatt cggtttgaag gtggaaatgc cgtctgaaat
     5521 caggtttcag acggcagt
//
LOCUS       DAWKOD010000134         5529 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000134 DAWKOD010000000
VERSION     DAWKOD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.134"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            65..490
                     /locus_tag="WJK73_001669"
                     /pseudo
     CDS             65..490
                     /locus_tag="WJK73_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            496..1726
                     /locus_tag="WJK73_001670"
                     /pseudo
     CDS             496..1726
                     /locus_tag="WJK73_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1784..2404
                     /locus_tag="WJK73_001671"
     CDS             1784..2404
                     /locus_tag="WJK73_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7558121.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2415..2546
                     /locus_tag="WJK73_001672"
                     /pseudo
     CDS             2415..2546
                     /locus_tag="WJK73_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2543..3037
                     /locus_tag="WJK73_001673"
     CDS             2543..3037
                     /locus_tag="WJK73_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ7558122.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(3171..4832)
                     /locus_tag="WJK73_001674"
     CDS             complement(3171..4832)
                     /locus_tag="WJK73_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ7558123.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMIAAEAVFPLLEHLEEAEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4962..5505)
                     /locus_tag="WJK73_001675"
                     /pseudo
     CDS             complement(4962..5505)
                     /locus_tag="WJK73_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
BASE COUNT         1331 a         1508 c         1402 g         1288 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacagtgt tttttaccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggcaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
      421 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc
      601 gcaaccatcg aaggggacag gagcggccgc accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc cagcaatgga tgccggtatt
      721 tatctcgaag aaaccgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
      781 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc acgagaataa aattgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcacccggc
      901 gcgcaggtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat aggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggca
     1201 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
     1321 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtattgcgcc tcttgatgaa cagtctcgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcccgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ttgctcaaag aagccgaaac
     1681 gcggcagtcg gaatggggca gggcggaaaa cggttctttg aactagtctg tttcagacgg
     1741 catccggatt caaatgccgt ctgaaaacac aaaaggaaaa accatgacca cacatcatgt
     1801 cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcaacc aagtcgattt
     1861 ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg gcaagtccac
     1921 cattatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga tgcttttggg
     1981 cgaacgtacc ggcagcaaag cgggggcgcg gcttcgcagc caaatcggct acctgcccga
     2041 aaccgttgcg ctgcaccctt cgctgaccgg catcgaaacg ctggattttt atgccaaact
     2101 taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca tttcacaggc
     2161 ggcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg ccttggcaca
     2221 agccctgctg ggcgagccca aagtcctgct gtttgacgaa ccgacaaccg gcctcgaccc
     2281 tgcatcaagg caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg gcgcgaccgt
     2341 attgctcagc acccacgccc ttgccgagtt ggacggacac gccgaccgca ttatcgtgga
     2401 ttaaatttaa tccactatat gcgggtatgg cgggtttgag cggacaaatc agcctgaccg
     2461 tccccgtttt gctgaccgct caggttttat gggttatcat tccgcttgtt ttggcagccg
     2521 gaatttttag aaagcgacaa atatgaaaaa aaccctgttg gcaattgttg ccgtttccgc
     2581 cttaagtgcc tgccggcagg cggaagaggg gccgccgcct ttaccccggc agattagcga
     2641 ccgttcggtc ggacactatt gcagtatgaa cctgaccgaa cacaacggcc ccaaagccca
     2701 gattttcttg aacggcaaac ccgatcagcc cgtttggttc tccaccatca agcagatgtt
     2761 cggctatacc aagctgcccg aagagcctaa aggcatccgc gtgatttacg ttaccgatat
     2821 gggtaatgtt accgattgga caaatcccaa tgccgacacg aagtggatag atgcgaaaaa
     2881 agccttttat gtcattgaca gcggttttat cggcggtatg ggtgcggaag acgcactgcc
     2941 gttcggcaac aaagagcagg ctgagaaatt tgcaaaggat aaagacggta aggttgtcag
     3001 tttcgacgat atgcctgata cctatatttt caaataaatc caaacaaaaa tgccgtctga
     3061 acgttgaagc caaggctcag acggcatttt ttgtcatagg ttcaaacggg gcaggtttga
     3121 acaactgccc cattccttat tcctgaccgg gcgtaacggc ggaataaggt tcacatcccg
     3181 ccgtaattcg gcccgcttgc gccttcggga caaatccaag tgatgttttg cgtcgggtct
     3241 ttgatgtcgc aggttttgca gtgtacgcag ttggcggcgt tgatttgcag gcgcggactg
     3301 ccgttttctt cgacgatttc atacacacca gccggacaat agcgcgtttc gggcgaggcg
     3361 tattctttat agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
     3421 tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
     3481 acgccgtcgg gtttcggata atcaatcggt ttgcacgcgg cggctttttt gagctgctcg
     3541 ttgtctttgc cgtaatgttt caaggtccac ggggctttgc ctctgaaaat catctggtcg
     3601 atgccggtat agattgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
     3661 gcggcgtaaa gctctcgata cagccagctt tgttcaaaac gttgctgata atccgccgct
     3721 tctttgccgc tgtcgaaacc ctccgcttct tcaaggtgtt ccaacaaggg gaacacggct
     3781 tcggcggcga tcatagcgga tttcatcgcg gtatggatgc ctttgatgcg cggcatattg
     3841 aggaaacctg ccgcatcgcc gattaaaacg ccgcctttga acgagagctt cggcaaactt
     3901 tgcaaaccgc cttcaatcag cgaacgcgcg ccgtaagcaa tgcggcggcc gccttcaaag
     3961 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
     4021 ggattttgat agtccaaacc gaccacgaag ccgacggcga ctttgttgtc atcgaaatgg
     4081 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcaccacc
     4141 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
     4201 gtttgcggct ggctgttttg gtcgagtttg aagcgttcga tgatttgttt ggaaagcgaa
     4261 ccgcgacaac cttcggcaaa cagggtttgc tgcgcccaaa gctccatgcc gggttggaat
     4321 gaatcggtcg gctcgccgtc tttgccaatg cccatattgc cggttgcaat gcctttgacc
     4381 gaaccgtctt cgtgatacag cacttcggcg gcggcaaagc ccggatagat ttccacgccc
     4441 atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
     4501 ccgtgattgt cgaaatttgg ggtaataggc agattgaacg cttttttctc agtcaggaac
     4561 aacactttgt cctgcgttac cgtacgcgtc agcggcgcgc ctttttcttt ccagtcggga
     4621 atcaactcat tcagcgcaat cggatcgata accgcgcccg ccagcgaatg tgcacccgcc
     4681 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgttctgttc ggcaagctgc
     4741 ttgagtttga tggcggcgga caaacccgac gggcctgcgc cgacaatcac gacatcgtat
     4801 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
     4861 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
     4921 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
     4981 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
     5041 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
     5101 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
     5161 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
     5221 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
     5281 agggcatagc ctttcgcttc aaaacccaaa tcaggcataa tgcgccgcat atcctcaaac
     5341 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
     5401 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
     5461 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
     5521 agcaacagg
//
LOCUS       DAWKOD010000135         5453 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000135 DAWKOD010000000
VERSION     DAWKOD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.135"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            64..324
                     /locus_tag="WJK73_001676"
     CDS             64..324
                     /locus_tag="WJK73_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558124.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WJK73_001677"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WJK73_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ7558125.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1091..1480
                     /locus_tag="WJK73_001678"
     CDS             1091..1480
                     /locus_tag="WJK73_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7558126.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEVRQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WJK73_001679"
     CDS             1558..2388
                     /locus_tag="WJK73_001679"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ7558127.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKHVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WJK73_001680"
     CDS             2403..3860
                     /locus_tag="WJK73_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ7558128.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILSAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WJK73_001681"
     CDS             complement(3901..4290)
                     /locus_tag="WJK73_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558129.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALMGIAFLRGVDVYDPPVSKEK
                     ALKALEKHPEIYHKFKRFFPFVELPLF"
     gene            complement(4472..4873)
                     /locus_tag="WJK73_001682"
     CDS             complement(4472..4873)
                     /locus_tag="WJK73_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558130.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEENNSDGNPTANYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(4981..5160)
                     /locus_tag="WJK73_001683"
     CDS             complement(4981..5160)
                     /locus_tag="WJK73_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558131.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHCHQPFEDRME"
     gene            complement(5179..>5453)
                     /locus_tag="WJK73_001684"
     CDS             complement(5179..>5453)
                     /locus_tag="WJK73_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558132.1"
                     /translation="EYWKLENALIEVRRKYPYPMDIPRDIVIGIGSIIEFLMVPNWKL
                     FTIKSSPWLPKSIKINERYERFRVMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT         1352 a         1609 c         1148 g         1344 t
ORIGIN      
        1 gcaaacaaat cggcggccgc ccgggaaacc ccccccgaac gcgtccggaa aatatgctta
       61 tcgatggaaa acgcagccgc atcccccgcc gggcgtttca gacggcacag ccgccgccgg
      121 aaatgtccga cgcttaaggc acagacgcac acaaaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccac caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataataa agcagaattc tttaacgaat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccgc actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccaccggcct cggcgtattg gcgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgtcggcgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagtgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg cgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcatcgc caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aacacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tggaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gcttcaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggcggcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcaatggcg accctctcgc
     3061 tgggcggatt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgagg
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt tccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ttggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatctttac cgtcttcatc ctcttcaccc
     3841 ctgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atattgtctt
     3901 ttaaaaaagc ggaagctcta caaaaggaaa gaaccgttta aatttatgat aaatttcagg
     3961 atgtttctct aaggctttta atgctttttc ttttgaaacg ggagggtcat agacatctac
     4021 cccccttaag aaggcaatgc ccattaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctatactc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg agtatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt ttccgtcaaa cactcctttg gttacctgct
     4381 ttgtatcaaa aatgccgtct gaaagccgaa tattgtttca gacggcattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct
     4561 tttcaaaaaa atcaaaactt tcttctttca accagtccat ataactgtca tctattatct
     4621 cgaaaaaata atttgcagta ggattcccat ctgaattatt ttcttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catctgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tctacgtaaa atctgcgact attgttagaa aattgtaatg
     4861 gtttaatctt cactatcgtt ttccatatct gatttttgtt ttggtaacac gacgatcggg
     4921 gtttcagcga caccgaggta ttttaccgtc taatcgtgga gattttaccg attttctttc
     4981 ctattccatc cggtcttcaa atggttgatg acagtgtttg cttttcagcc gtctgccgtt
     5041 ctgaccaagg tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac ttagttccca
     5101 atatcctgtt tcaaataagg ggaaggtcgc ctgaaaatta aacacggttt cagacggcat
     5161 tttgattgcc ggggtttgct attttttgtt gtaataatca aatcgtacgt tgactatgtc
     5221 tttctcggta aaaatataac ggagcattac tctgaacctt tcataacgct cattaatttt
     5281 gatactttta ggcaaccaag gggaagattt aattgtaaaa agtttccaat ttggaaccat
     5341 taaaaactca ataatgctac cgattccaat cacgatatcc cttggtatat ccatcggata
     5401 aggatatttt ctcctaacct cgattaaagc attctccaat ttccaatatt ctt
//
LOCUS       DAWKOD010000136         5401 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000136 DAWKOD010000000
VERSION     DAWKOD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.136"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(99..2933)
                     /gene="lbpA"
                     /locus_tag="WJK73_001685"
                     /gene_synonym="tbpA"
     CDS             complement(99..2933)
                     /gene="lbpA"
                     /locus_tag="WJK73_001685"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ7558133.1"
                     /translation="MNKKHGFQLTLTALAIATAFPAYAAQAGAAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSQDKTGQFFLVEGENTL
                     KPVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEST
                     GFDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2930..5149)
                     /locus_tag="WJK73_001686"
     CDS             complement(2930..5149)
                     /locus_tag="WJK73_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7558134.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNIATSDKDGNDFPNSRQAEEKLSFKEG
                     DVLFLYGSKKDQRQQLEDEIRQRNPNVQIRTSENENKKYGYNFVDAGYVYVKGEDEIE
                     RTSNYKYFTHRFGYDGFVYYSGEHPSQSLPSAGTVKYFGNWQYMTDAIRHRTGKAGDP
                     SEDLGYLVYYGQNVGATSYAATADDREGEGKHPAEYTVDFDKKILEGKLIKNQYVQRK
                     TDQKKPLTIYNITAKLDGNRFTGSASVNPDLAKNHANKEHLFFHTNADQRLEGGFFGD
                     NGEELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEAS
                     DKNPREFAISSMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMTVSACCDFLTYVK
                     LGRIKTDRPAAKPKAQDEEDSGINNGEESEDEDEVVEEEAEEEADEAEADEAEDEEAE
                     EIEEEAEEEETEEKSPAEGNGGSGSILPAPEAPKGRDIDLFLKGIRTAETNIPQTGKA
                     HYTGTWEARIGTPIQWDNQADKEAAKAKFTVDFGGKSISGTLTEENGVEPAFYIEKGV
                     IDGNGFHATAHTRDTGINLSGTGSTNPQRFKASNLRVEGGFYGPQAEELGGIIFNNDG
                     KSLGITEGTENKVDVDAEVDAEVDAEVDVDVEQLKPEAKPQFGVVFGAKKDNKEVEK"
BASE COUNT         1180 a         1443 c         1236 g         1542 t
ORIGIN      
        1 atcttgttta tatcgaatca aaaaaacctg caccttaatc agttggcggt ttagtccgac
       61 ttttggggtg cagatcaagc tttcagacgg tatttccttt aaaacttcat ttcgagcgcg
      121 aggctgaagt tcctgcccgg tgcggcatac cttccatagt tgctgtcgcc gccgtgccgg
      181 tttgccgtgc tttccgcggt ctggcgcaag gattcccaag taacgtagcg gtagttgccg
      241 atattgtaga tagccgccct caaggtcagc cgttttttca gattcagata ggcggaaacg
      301 tctgccgtcg accaagaaga cgacgctctt tttgtcgaat atcgtttttg atcgcctgcc
      361 agataagcaa gctcgtcagg gtttttccct ttggaatagg tcagcataat gtttgcgccc
      421 catttcccct ctggctggtc gtatccgaac cccaaaacat aacgcgacgg ctgtaccgca
      481 tccaaagcat agctgcggag ggacagtccc ggccggttgg ataccgattt cggtttgatg
      541 cggttgtacg ccaaagtcgt gtacaaacct tcgggcagtt tgccatacac gccgttccag
      601 tcgatttttc ccaatatatt aacgccttga agcgacatat tttgggcatt gtaataatcg
      661 cgtatatcaa tctctgtcaa ttgtcctgcc tgattcggca atttggtttt gtgatcggca
      721 acggcaatca tatcggtata acggttgcgg aagctgctga tttccaaaaa gccgaaatcg
      781 cccttccact gcaaaccgat ttcgcggttg gctgcctttt ccgatttcag ggcgggacgc
      841 tgccagcctt tcggataatc gtgataaatg tctatcccga aaagttcttg gaatgagggc
      901 gttctgaagc cgctggaggc gcggtaagac aaagaaaaat gccggttcgg tttgaacacg
      961 atgccgctgt tccacgaacg gtcaacatac cgcccgctgc ggacgagttc ttccgacgtg
     1021 gtgaagtttt tccggtcata cctgccaccc aagctgaaat caaaatattt gccgattgaa
     1081 aaacggtcgt tcaaagaaat atggatattg ctgccgttga tttttcttgg cacgcatttg
     1141 cgggaacgca gggtttcgat gtagccgcag accgaccctt cgacgacttc gggcttaccc
     1201 aaacggtact tatcttgatt cttttcatcg aatcccgtgg attccgaaat ccttgccgca
     1261 ttgtgggaaa gctgttcagg gcgggagatc gcgttggaag catcgtaacc gaagcccaaa
     1321 gtcagatggt gtttcgtcca tttgtttttc agcgacttct caaacgaggc attcaaaaca
     1381 ttgtgctgtt cgcggtagtg gaaacggtcg ctgctgtcgt aggaatacgg tttgtccgcc
     1441 gacgcgcggc aggatttgtc cacagcagga tacacggcgc aattcagctt cagcgtgttg
     1501 ttgtcggttg ccacgccctg tttgtcaaac gacaacaccg ccttatccgc ccagttgtca
     1561 gaatacgctt cgttttcata acggtacagc aaacccatac ggcggcggcg gtgatgttca
     1621 tcaataaatt tggtgcggga atatttcaaa cctatgcccc tgaccaactt tttatcgccc
     1681 ttccactctt ctatattcgg cacaaaatac aagccgtcgc ggaaatcgtc gccgtcgtac
     1741 accccgctct tgtctctaaa cttgtccgcc tcgtccgtac cgtaatactg tttttccgtc
     1801 atatcgcgga tatcgtaacg ctgtttggta tcctcaaaca cgccgccgac ataatgcctg
     1861 ccgccgaagc ggtagcccag cttggcaagc caagagccgc tgcggtaatc catcggatcg
     1921 ggcaatatcc tgccgccgcc cgtgtaagct tgggcggaca ggttttcgtg gcgcgcctgc
     1981 gcctcccgca ccatcgcttc ttcttcagca cttaaaggct gatttttttc aatacgttct
     2041 tttacccagc ggttaagctg gcggttcaaa tatatcccgt agcccgccaa ttttgccacg
     2101 ggcttgagtg tattctcgcc ctctactaag aaaaattgcc ctgttttatc ttggctttga
     2161 atatcgtgtg tctggcggaa cgcgtccaaa cggtttatgc cgtatgccac cccgtccgca
     2221 atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg ttcggattaa cagcccttcc
     2281 caaccgtctt tgctgaaccc cgcgccgagc gacttcataa attggcggtt tttactgccg
     2341 taggcggttt ttgcctgtat cccccagctt ttgccgtctg aaatcaggtc tgccgcttct
     2401 ttggtgcgga aggcgaccgc gccgccgagt gcgccgctgc cgtgatcgga cgaaccggca
     2461 cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat tgcgccgctg
     2521 ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg tatttgggca
     2581 acgccgtcaa ccgaaaccgc cacacggttt ttatccacgc cgcgtatcga gtagccgccg
     2641 ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt caggtcgcgg
     2701 ataccgagta cctgttcttt gttcaacgtt tccgacgttt tgacgatttt gcccaaaccg
     2761 gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc tttcagggat
     2821 tgggtctgcg cggcatcagg tgccgccgcc cccgcttggg cggcataagc cggaaaagcg
     2881 gttgcaatgg ccaaggcggt cagggtaagc tgaaaaccgt gtttcttatt catttttcca
     2941 cctctttatt atctttcttc gcaccgaata ccacgccgaa ttggggttta gcttcaggtt
     3001 ttaactgttc aacatcaaca tcaacttcag catcaacttc agcatcaact tcagcatcaa
     3061 catcaacttt attttcagta ccttcagtta taccaagaga tttcccatca ttattgaaaa
     3121 taataccgcc caattcctcc gcctgcgggc cgtaaaatcc tccttctaca cgaagattac
     3181 tggctttgaa acgttgagga ttagtcgaac cagttcccga aagattgatg ccggtatccc
     3241 gagtgtgtgc tgtcgcgtgg aaaccgttgc catcaatcac acctttttca atatagaaag
     3301 caggttctac accgttttcc tccgtcagcg ttccagaaat cgatttcccg ccgaaatcaa
     3361 cggtaaattt tgcttttgcc gcttctttat ccgcctgatt gtcccattga atgggtgtgc
     3421 cgatacgcgc ttcccaagtg ccggtatagt gtgcttttcc agtttgtgga atattcgttt
     3481 ctgccgtgcg gatacctttc aggaaaaggt cgatgtccct gcctttaggg gcttccggag
     3541 cgggcaggat gctgcctgaa ccgccgttgc cttctgccgg cgatttttct tcggtttctt
     3601 cctcttcagc ttcttcttcg atttcttcag cttcctcatc ttcagcttca tcagcttcag
     3661 cttcatcagc ttcttcttcg gcttcttctt cgacgacttc gtcttcgtct tcgctttctt
     3721 caccgttatt aatacccgaa tcctcttcat cctgcgcctt cggtttggcg gcgggacgat
     3781 cggtttttat ccgtccgagt ttcacatagg tcaaaaagtc gcaacaagca ctgacggtca
     3841 ttttcctgcc gtcggcaaga tcaatggttt gagtatcttt gaccaaggag atttcacgcc
     3901 cttcgacaag aagcttgtcg ggatgaccaa aatcgggcat agaggaaatg gcaaactcac
     3961 ggggattttt atcacttgcc tcgtcaacgg aaatttttag agaatccaag attttggtgt
     4021 gttttccaga cggcagggca ggatttgtat ctgatgcgtt tgctgtctct gttttttgtt
     4081 tgcctgcgaa tacgccgaat acgcttttgt cgttgctgat aaatcgtccg gcaagctctt
     4141 ctccgttatc gccgaaaaaa ccgccctcaa gccgctgatc ggcattggta tggaaaaaca
     4201 aatgctcctt attggcatgg tttttcgcta aatcaggatt gacactagca ctgccggtaa
     4261 agcggttgcc gtccaatttt gcagtaatgt tgtaaatggt cagtggtttc ttttgatcgg
     4321 ttttcctttg cacatactga tttttaatca gcttgccctc caggattttt ttgtcgaaat
     4381 caaccgtata ttcggcagga tgttttccct ccccctcccg gtcgtcggca gtcgcagcat
     4441 aagaagttgc tccgacattt tgaccgtaat aaacgagata acccaaatct tcgctaggat
     4501 ctcctgcttt tcctgtccga tgacgtatgg catcggtcat atattgccag ttaccgaaat
     4561 attttaccgt tcccgcgctc ggtaaagatt gcgaaggatg ttctccggaa taatatacaa
     4621 aaccatcata accaaaccgg tgagtaaaat acttgtaatt tgaagtcctc tcaatttcat
     4681 cttctccctt tacatataca taacctgcat ctacaaagtt ataaccatat tttttatttt
     4741 cattttctga tgtcctaatt tgtacattag gattgcgttg acgaatttca tcttcaagct
     4801 gctgacgttg atcttttttg gaaccgtata aaaacagaac atcaccttct ttaaacgaca
     4861 gcttttcttc tgcttgtctg ctatttggaa aatcattgcc atccttatca gaagttgcga
     4921 tattccgcct caacagccgc attgccgcac cgacggcggg ccggttgacc ggcgtggttt
     4981 ctaccgaagg cccggcaggg ggcgaagtgg gaacgtcctt agacttgaaa gtgacggggt
     5041 acgcggtcgg cgttgattcg acaacaggct gcacgccgaa attgccgccg atacaagatg
     5101 ccaaaagtaa gggcaacaag acaatgccgc cataattcgg tttacacatc cctacttttc
     5161 ctctatttga ttaataataa ttatcattat attaatatgt acagataata tcaagccgtt
     5221 tttatagtga attaacaaaa atcaggacaa ggcgacgagc cgcagacagt acagatacat
     5281 cccgtcattc ccacgaacct acatcccgtc attcccacga acctgcacca cgtcattccc
     5341 acgaaagtgg gaatccagtt cgttcggttt cgcttgtttt aagtttcggg taacttctac
     5401 t
//
LOCUS       DAWKOD010000137         5367 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000137 DAWKOD010000000
VERSION     DAWKOD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.137"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..509)
                     /locus_tag="WJK73_001687"
     CDS             complement(126..509)
                     /locus_tag="WJK73_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558135.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(514..1021)
                     /locus_tag="WJK73_001688"
                     /pseudo
     CDS             complement(514..1021)
                     /locus_tag="WJK73_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(1653..2048)
                     /locus_tag="WJK73_001689"
     CDS             complement(1653..2048)
                     /locus_tag="WJK73_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558136.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2060..2773)
                     /locus_tag="WJK73_001690"
                     /pseudo
     CDS             complement(2060..2773)
                     /locus_tag="WJK73_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2869..3141)
                     /locus_tag="WJK73_001691"
     CDS             complement(2869..3141)
                     /locus_tag="WJK73_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558137.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(3329..3817)
                     /locus_tag="WJK73_001692"
     CDS             complement(3329..3817)
                     /locus_tag="WJK73_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558138.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKQDFINNERYEKTELYPQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(3814..>5367)
                     /locus_tag="WJK73_001693"
     CDS             complement(3814..>5367)
                     /locus_tag="WJK73_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7558139.1"
                     /translation="AYDKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAA
                     GAGAGGAAAGTAAGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLIN
                     NKGDINHTLKELGKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLT
                     ANLINSTAAASVHTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKI
                     AHAIAGCAAAVANKGKCRDGAIGAAVGEMVGETLLDGHDVGKLSPQERQKVIAYSQII
                     AGSAVALVKGDVNTAANAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITN
                     QIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQYYNQLRESRGIPVLPIYRMRINNGNE
                     FNRIMSSKYPYNELYVANPKSVTGHFRVDSYNPATEEIISRKFTQFSQIQESTGIGYI
                     KEAVRKYSPGAVISNVPSTPTTIRGRKLEGKLILEVPAQVNPIPQSVLRAAQEENVII
                     RDTTGRIYK"
BASE COUNT         1446 a         1161 c          966 g         1794 t
ORIGIN      
        1 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagcttgc tgctgcggta
       61 taaaagtatt tttagtgatt tactgttttt ggtatttttg cccgacgggg ttaaaaatac
      121 agttgctact cttgctgcat gatattggca agatttaatt taaatgtttg ttgaatcttg
      181 tttacatcct tagcaaaaat atgatagtca ctcgaagaat agatagattt tattctatcc
      241 aaattagttt gttgataaca ttttttcatt tccgcgatga aaaaattatc ttttcctctt
      301 tcattgagac agttattttc taacagatag attgcccatg ctgtaaaaat aaatgtacta
      361 tcttccggat attcagataa ataaatttcg aattcaatta tagaatattc agctgcttca
      421 attaatgtta tatctaaatc cctgacaaat ttttccgtta tatttttaaa taatttcttg
      481 aattctatat aattttttat tttttccatg attctatttt cctgtaaaaa tatttaattg
      541 aatatttgga tagcgatttc taaactctaa aataacattg ctgcaagatt gacaggcctt
      601 taattctgta aatagatcaa ttctacctga tacattacga ttatttccga gttgctgtgc
      661 tatagtttca agtaatttat attctccatc tatatttctt aatgttcctc taggaggaga
      721 ggcaatatta tgatatttat caacagatat aggtttaaaa atttttgttt caggtaacga
      781 aataaatcca tgttcccctt tttggaaaga actaaatgct tccatccttt gcggtaatcc
      841 tttaacatca atttttgcta tcgcaagatt ccctatttgt cgatactctt ccggcagttt
      901 catgcggact ctctggcttt cagcagttaa aagcaggagc actgtcattg ctcagataac
      961 gggtaacagg tgtaggcttg gccgatattg cagcctgtgc agatacagat cctgcaacca
     1021 tcaatgtgcc attaacaacc tgaactgtac catgcagata ttctccatta gagattgctt
     1081 tcgctccctg tccaatgtta tatccccctg ctgccacagt agctgctttg acactgaagc
     1141 caagcggtcc gtttaataag taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa
     1201 aagaatcata acgactcaat gtttgatagg ctttcgataa agtgttatta atatctttgg
     1261 aaggaatagt gagattaccc attgactggg gcgttttcag tatcgaagct gcccaagctg
     1321 tgttacctcc agaggcttgg ttaataaaat atgttaccta tgtatattgt ttcttcaaaa
     1381 atcaaccgta ttgtccttgg gtgattttcg taccagcaca atcagtctgc cctagttgac
     1441 agatcgcgcc acactagcca gatacagcgt atcattggta aaactgataa tgcggcaata
     1501 taaagaaaaa cttcaatagt atgttaacga aatttgacgc ataaaaatac gctaaaaaat
     1561 tttcgcttga ctaattaaaa atgggattga gcataaaatt ttaataattt attatattat
     1621 tgaaaatatc tcagattttc agtcagttta ttttataagc cgaaccgtag gctattttcc
     1681 atctttaacc atgaatagac ctcaacttca tcataatttg tgcctgcaat agcaacttcc
     1741 agaaagtcaa taattctacc tgctttattc aaagactctg tagtatatag caggaaagtc
     1801 gctgcatctg acttatccgg tgcagtgatg tctccttcaa aaaaaatagg gatattttcg
     1861 tatttattta gattagttct ttctaaattt acctgaaaat caatgtagca cccacatcct
     1921 gtattatctc tagatactat tattgaattt tctatttgct ctattaaata ttccttccat
     1981 atcatattgt tttcaataat tctacataag aaagccttct catattcaga taaacaaaac
     2041 tggttcatat attctaccct tacattttca aaatagttgc aatacgagat actccattgt
     2101 tgacatatac gcgaacaatt accgttatac cattgaccgt tacagtaact ggataatttc
     2161 ctgtttttgt cagttttaaa gcatccactg ccttttgcgt cgctacaaca gcatttccca
     2221 tggagcctga tttttgaatc aatgtattac cgatgcttcc ttgatgcccc ttaaagatgt
     2281 gatgaacttt atttagccaa tctacaccgt atctttcagc caatagtcga gaattacttt
     2341 cacctatttt ttccatcgaa gctaaaatcc tttgagcata ggcagcatca gctgttgaaa
     2401 aatttgcttc gcccaacaaa agccgtaatt cctgaatatc tcctcgtaaa attgcatttc
     2461 ttactcccgc ttctaatgtg gtttgtggct gaaagaggga attattctct accgccacag
     2521 tagccgcatt cgccgctgta ttcacatccc cgcctacaac ggcagacgca gtgccggcaa
     2581 ccagttttga ataggcagta atcttcgctt tagctttttc gatttcggtc gcactcatac
     2641 gactgaaatc agtattctta accaaagcct caccaacaat ctcaccgact gcagcgccta
     2701 tcgcaccatc ctgacactta cccttattcg ccgtcgctgc tgcacagcct gctacggcat
     2761 gggcaatctt gtgggtggta tatcgctgcc agccctctct gttgataatc ttgcgtatga
     2821 ttacgccgtt tgatggctaa tcgtacctac gcaggcttaa aatttccatc aatccatatc
     2881 atttgagttt tcatcttcat aatatggatg atcaaacaaa tctccttccc aaatacattc
     2941 actcgttata ccttgggatt ccatgaaagg aacataagaa taaaaatctt tttcatattc
     3001 tccataaaat atattcatac ctttgagcca tactgcatcg cattcatcac acagaataat
     3061 ttcatcatga agatatttag gtttcgaatg gtagagatag ccttgttgac aagcaggaca
     3121 cattttcatt ctcttattta tgatcattcc atataatcaa tttatttttg tgggtaagca
     3181 atagtgattt gattgctgcc tttagtctgt acaacaatac gtatatagca atctgataat
     3241 ttcctggttt ttatcagctt tatcctcaga ttccaaaccc aataataaaa gccgtttgaa
     3301 atttttttca gacggcctta aaacttaatt aaataatgtc cggatttaac accaaatcat
     3361 attcaccaaa ctttggaaat agaattacct tatctttttg gggatataat tctgtttttt
     3421 catatcgctc attattaata aaatcttgtt tcaaaacata gcaatcatcc gtattttcat
     3481 tgaagaaata agtatatcct tctgctaagt catgagattc caaagttata taatctctcc
     3541 atacagccat agaaagaaaa taataattcg aattggaata tttttgaaat tgatgggtta
     3601 aataatccct tccaaattta tcgaaaaaat gtaccgaaaa aaaacctact tcctctcgca
     3661 aggtaattac atattcaggt tgtgtcgccg aacctattac ggcagtatat aactcccctt
     3721 taagatgttt ttcctcagct tgtttctcag aaaaaggctt atgaagtttc ttataaccat
     3781 aagaccactg ctcacaataa aaaatatctt tcttcatttg taaatccttc ctgttgtatc
     3841 tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa ttggattgac
     3901 ctgagcagga acttctaaaa taagttttcc ttcaagcttt cttcctctta tcgtagtagg
     3961 tgtacttgga acattggaaa tgacagcacc agggctatat tttctaacag cctccttgat
     4021 ataaccaatc cccgtacttt cttggatttg agaaaattgg gtaaattttc ttgaaataat
     4081 ttcctctgtc gcaggattat acgaatctac cctaaaatgt cccgttaccg atttaggatt
     4141 ggctacataa agctcattat aagggtattt tgatgacatg atacggttaa attcattgcc
     4201 gttgtttatc ctcattctat aaattgggag aacaggaatg cctctggatt cccttaattg
     4261 attataatat tgcctgtatg tttgtacatc atgtcttgtc cacggctctc taggattcct
     4321 cataatagca atcccgttaa atttcggatc cagccttcgg atttgattgg taatggcctg
     4381 gatttcaaga atggcatatt catgttccaa ttcttgtcgc ggagtccaac gtatatttac
     4441 cctcctgcga gctaaaagac tattattctc cactgccaca gtagccgcat tcgccgccgt
     4501 attcacatcc cctttaacca atgccactgc gctgcctgcg ataatctgcg agtaggctat
     4561 gactttttgg cgttcttggg gtgacagttt gcctacatcg tgtccgtcca acagggtttc
     4621 tcccaccatc tcgccgactg ccgcgccgat tgcgccgtcc cgacatttgc ctttatttgc
     4681 taccgctgcc gcacagcccg ctatggcatg ggcaatcttg tgggcaatgt agtcttcgct
     4741 gagattaaat ttgattttgc tcgctacttc tccgtgtacg gtactgacta tcgcacccag
     4801 tgcggcatcg cccaaattgt ctttcaggct gccgccgttg atggcggtat ggacacttgc
     4861 ggcagcggtg ctgttgatca ggttagcggt cagtttgcct gctgcgggac tgtgaaaatg
     4921 tttgctgacg gcttcggctg cttgggtgtt cagcccgctt atgccctgca gtacgcctgc
     4981 ggttacggcg gcggtggcgg cctgtctgac ggtgctgctt ttgcccagtt ctttcagggt
     5041 atggtttatg tctcctttgt tgttgatgag ggaaactgcg gcttggctgg cgagactggc
     5101 cagtgcggct ttgcctgcgg ctgtggtgat agctgcagct gatgtgcctg ctgctactcc
     5161 ggttgtggct gccgttcctg ctgctactcc agttccggct gccgttcctg ctgcggctcc
     5221 tcccgctcct gcgcctgccg cgcccgccgt tccggcggct gccggggcgg acagtgcacc
     5281 gtaggtcaat acggttacga cgataacgac gacagctgct gctgcgggtg tcatgccctc
     5341 ctgtttgtag tcccatttat cgtaagc
//
LOCUS       DAWKOD010000138         5360 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000138 DAWKOD010000000
VERSION     DAWKOD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.138"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJK73_001694"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJK73_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ7558140.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1383..2147
                     /locus_tag="WJK73_001695"
     CDS             1383..2147
                     /locus_tag="WJK73_001695"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ7558141.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2167..4182
                     /gene="rep"
                     /locus_tag="WJK73_001696"
     CDS             2167..4182
                     /gene="rep"
                     /locus_tag="WJK73_001696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ7558142.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WJK73_001697"
     CDS             complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WJK73_001697"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ7558143.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         1238 a         1476 c         1482 g         1164 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg gatgtgtatc
     1201 gttttcccga tgtgcgggct atcggtaatg ctgcttcacg aggttgccat tatcgcatat
     1261 ttccttgctt gccgatatgc gccaggacgc ggcggcttgt gccggaatgg aatctggatg
     1321 ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgtg tgtgcctgtt
     1381 ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc accaaaatcg
     1441 ccgtgccgct tgtcgcccgc gatgccgccg aactttccgc cgtacttgag caaatcaaaa
     1501 atatgccctt cgatattgcg gagttccgcg ccgacttttt ggaatgcgcg ggcagtatcg
     1561 gcgaaatatt gcaccacacg cagaccgtcc gcgacgcgct gcccgacaag ccgctgctgt
     1621 ttacgttcag acggcatggc gaaggcggct cgttcccgtg ttcggacgat tattatttta
     1681 aactgctcga cgcgctgatc gaaagtcgcc tgcccgacat catcgacatc gagctgtttt
     1741 ccggcgaaac cgccgtccgg tgcgccgtgg caaatgctca aaaaaacggc atcgccgccc
     1801 tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta tgccgtctga
     1861 aacagatgga ggactgcggc gcggacatct gcaaaattgc ggtgatgccg caaagcgcgg
     1921 aagatgtgct gactttgctt tccgccacgc tcaaagcgaa agagcttgcc gccaaaccga
     1981 ttgttacgat gtcgatgggg cagacggggg cggtcagccg gcttgccgga caggtgttcg
     2041 gctcaagcat cacgttcggt tcgggaacgc aaaactccgc gccggggcaa atcggcgtat
     2101 ccgccctccg tgcgacactc gactgcctcg aaaacggagc agactgattt cagacagcat
     2161 caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct cggcggccca
     2221 ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca aaaaattaag
     2281 catttgattg tcaatgtcgg ctacctgccg cataccgttg ccgcaattac ctttaccaac
     2341 aaagccgctg cggaaatgca ggagcgcgtt gccaaaatgc tgcccaaacc gcaaacgcgc
     2401 gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga agaggcgaac
     2461 catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc gaaaatcatc
     2521 ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcagca ccagatttcc
     2581 ttgtggaaaa acgatttaaa aacgcctgaa gatgtcgttc agacggcatc gaacatttgg
     2641 gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag ctatcaggca
     2701 gtggacttcg acgacttgat ccgcctgcct gccgtgctgt tgcagcaaaa cagcgaagtg
     2761 cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca agatacgaat
     2821 acctgccaat ttacgttgat gaaacttctg accggcgcgg aaggtatgtt taccgccgtc
     2881 ggcgacgacg accagtccat ctacgcatgg cgcggcgcga acatggaaaa cctgcgcaaa
     2941 atgcaggaaa actatccgca gatgaaggtc atcaaactgg agcaaaacta ccgctccacc
     3001 gcgcggattc tcaaaatcgc caacaaagtc atcgagaaca accccaagct gttcaccaaa
     3061 aaactttggt cgaaattggg cgaaggcgag cccgtcaaag tcgttgcctg ccaaaacgag
     3121 cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat cggcggcgac
     3181 aaaacccaat atgccgattt cgccgtgtta taccgcggaa agcatcaggc gaggattttc
     3241 gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca aagctttttc
     3301 gacaaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa ccccaacgac
     3361 gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga tgtaacgctg
     3421 ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc cgcgcaaaac
     3481 gaagaagccc tcgccacgct gaacaatacc aaccgccaac acctgcaagc ctttatggat
     3541 atgttcggca gttaccgtgc caaagccgaa accggcgaag cgggcgagtt catcaacagc
     3601 ctgctcgaag aaatcgacta tgaaaaccac ctgcttgcca gtgaagaagg caaagctggc
     3661 gaaatcaaat ggcgcaacgt cggcgatttg gtatcatggt ttgcgcgaaa aggcggggaa
     3721 gacggcaaaa acatcatcga actcgcccaa accgtcgcct tgatgacgct tttggaagga
     3781 aaagacgaag aagaaaccga tgccgtctcg ctatccacgc tacacgccgc caaaggtttg
     3841 gagtatccgt atgttttcct tgtcggttgc gaagaaggcg ttttgccgca caacgacagc
     3901 atcgaagaag gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat cacccgcgcc
     3961 aaacgccaac tcacactgac ccactgcgtc aaacgcaaaa aacaaggcac atggcagttc
     4021 cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat cctgggtcgc
     4081 aaaggcggcg agccgattgt cagcaaagaa gaaggcagac gcaaccttgc cgatataatc
     4141 ggaaggctcg acaacctaaa aaaaagcggc gcggcggatt aaaccggagc cgcaatgccg
     4201 tctgaaggct tcagacggca tattttttgg acggcgcgcg taaagcggtt tacgcccaca
     4261 aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata aggcggaacg
     4321 cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc agcagagctg
     4381 cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg aaatcgtagg
     4441 tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt tgccgccaca
     4501 agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg tcttcgggca
     4561 gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg cgttcgtccg
     4621 taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt aagagttcgc
     4681 cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc atttttttca
     4741 gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa aatgccatga
     4801 ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac gcgattttcg
     4861 ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc gcaaaaatgg
     4921 cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa ttacgggtaa
     4981 cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa cgcctgaata
     5041 cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc acatacaccg
     5101 cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc ccgaactgcc
     5161 gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg acgaccaccg
     5221 gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaat gcgtccatgt
     5281 cgatgtggat gattttgcgt gaagacatcg gctctgctga ggataaaaag gatattctac
     5341 tgccggcatc gggcaaattc
//
LOCUS       DAWKOD010000139         5351 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000139 DAWKOD010000000
VERSION     DAWKOD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.139"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            57..339
                     /locus_tag="WJK73_001698"
                     /pseudo
     CDS             57..339
                     /locus_tag="WJK73_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(360..1586)
                     /locus_tag="WJK73_001699"
     CDS             complement(360..1586)
                     /locus_tag="WJK73_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ7558144.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1601..2026)
                     /locus_tag="WJK73_001700"
     CDS             complement(1601..2026)
                     /locus_tag="WJK73_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558145.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2131..2793
                     /gene="queC"
                     /locus_tag="WJK73_001701"
     CDS             2131..2793
                     /gene="queC"
                     /locus_tag="WJK73_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ7558146.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYRRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDEAAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            2821..3339
                     /locus_tag="WJK73_001702"
     CDS             2821..3339
                     /locus_tag="WJK73_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ7558147.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3346..3768
                     /gene="queD"
                     /locus_tag="WJK73_001703"
     CDS             3346..3768
                     /gene="queD"
                     /locus_tag="WJK73_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ7558148.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4037..4408
                     /locus_tag="WJK73_001704"
     CDS             4037..4408
                     /locus_tag="WJK73_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ7558149.1"
                     /translation="MKLLFIPLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4405..5043
                     /locus_tag="WJK73_001705"
     CDS             4405..5043
                     /locus_tag="WJK73_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ7558150.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
BASE COUNT         1379 a         1536 c         1334 g         1102 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttgatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta atattgcgaa
      181 ccgaaaatcc tatttttaga tgcagcgacc agccatttgg atattgccaa tgaaaaagca
      241 gtcaatgcaa acttgaatgg cttgtctatc ataaaaatta tggcggcaca cagaaaggaa
      301 acggtggaat cagcagatag gaaaatgtct ttaggataaa aatacagttt caaaaatact
      361 caagattgct gctgtttttt cgcctgagcg tcaaactctg ccagcgtcat gttcaaagtc
      421 tgcaaacacg gcatcattac tgcatcgaca gcttggttca catgatccct ttccacaggc
      481 aacggacggt aaacgaagag cttgaagagt tcgttcaact caatcgaatc cgcccccgtt
      541 ttcaacaccc aaccctgtct gccggaatag atgtagccgt gccgcgccaa cttttccaag
      601 agttcgccca actcgtcgta gcccatattg atatgccgtc tgaactcctg aacaggcaag
      661 gctttgcctt ctttttgcgc cgcatccaga agcagcagga ttttcaacac gtcgtcaaac
      721 cgtccgcgcg agtcgaagcc cctgcggaac gcttctccct gccagtagga gagtgaagaa
      781 gtcagcaccg cgccgcccaa gaccagcgtc cacaacaggt tcagccacaa cagaaaaaac
      841 ggcacggcgg caaacgcgcc gtaaatcgag cggtagccgt cgaagttgcc catataccaa
      901 gtgaagaggg aacgtgcggt ttccagacaa aacgctgttg ccaaagcccc gacaaacgcc
      961 tgccgcgcgg gaacgaagcg gtttggcacg aagcggtaca gcccccacag caaaagcgtc
     1021 atgaaggtca gcgtcgccgc cgttcgcaac gcgcccgacc actgcggcgc acctgaggca
     1081 agcgcggcat cctgtaccga gccgaccata aaggaaatgc ccacgcccaa agacagcggc
     1141 ccgaacgtca gtaaagccca atagacgaga aactgcatca tccacggacg ctgggaattg
     1201 acccgccaga tgcggttgaa cgtattgtct atcgtccgaa tcagcatcag cgaggtaacg
     1261 accagcatca cgctgccgat tgccgtcagc cggttcgcct gctcgcggaa cgcattgata
     1321 tagtcgaaca ccatgtccgc gccctgcggc acaatggttt ggttgacgaa ggagacgaac
     1381 gaatccgacc agcggtcgaa cacggggaaa atcgaagcga ccgccaccat cacggtcagc
     1441 acggggacga gtgccagcag cgtcgtaaac gtcatgcttg ccgccgcctg cggtacgcgt
     1501 tcttcatcaa agcggcggac gacgaaccat gcaaacgcac agattttatt gtctgccaaa
     1561 ccttgcaaac gttgtaaaaa ggtcataatt tcttgcccgg tcagtaagtt gggcattgat
     1621 gcccgatgtt atagccaatt ttgccgtcag gaacaaatgc ctgaactgcg gctgtttcag
     1681 acggcatcgg aacaactgtt atgccgtctg aagaccgaac cattttaacg gaatccgccc
     1741 atgaacccaa atccccctca aaatcctcgt cctctactat ttccaaaacg gcagcacccg
     1801 caatcccgca ctccgaatcg ctcgcggcat cgacagcgtt gaaggttgcg aagccgtatt
     1861 gcgcaccgtc cccaaagtct ccgccgtctg cgaagccgtc aaaaaagata ttcccgacag
     1921 cggctcccgt cctgaccgcc gaagaaaaca atatcgcctt cgcacaaagc aaacgcttgg
     1981 cggaactcgc cgtcaagtcg gcataagccg cgtgttcaga cggcatggcg ttcagatgcc
     2041 gtctgaacgc gtttgcctgt ataatccgcg tctttactat ccaacttcgc ggttcgcaaa
     2101 cctcccgcgt taccaaaact aggattcgat atgtcaaacc aaaaagcctt ggtcatcttt
     2161 tcgggcggcc aagattccac tacctgcctg attcaggcaa tccaaactta cagacgcgaa
     2221 aacgtccaag ccatcacctt ccgatacggg caacgccatg ccgttgagct ggaacgcgca
     2281 gagtggatcg cgcaggattt gggtgtcagt caaaccgtac tcgatttgag cctgatgcgg
     2341 cagattacgc acaatgccct gatggacgaa gccgccgcca tcgaaaccgc cggcaacggt
     2401 gttcccaata cctttgtgga cgggcgcaac gcgcttttcc tgctttacgc cgcgatttac
     2461 gccaaagggc aggggatacg gcacatcatc gcgggcgtgt gcgaaaccga cttctccggc
     2521 tatcccgact gccgcgacgt gtttgtcaaa tcgatgaacg ttacccttaa tttggcgatg
     2581 gactatgatt ttcaaatcca cacgccgctg atgtatctga ccaaggcgca aacttgggcg
     2641 ttggcggacg aaatgggcgc gctggactat atccgcgagc aaacccacac ctgctataac
     2701 ggcatcgtcg gcggcggctg ccgcgaatgc ccgagctgta tcttgcgcga acgcgggctg
     2761 gcggaatatc tggaaagtaa aaaggccgtc tgaacacgcg caaaccataa ggaatacgat
     2821 atgcccaagc tccatatgtt ttacctcggc ggcaatgccg gcaggtcgaa tatcgaagtg
     2881 cacgacatcc aatttgccgt gtgcgacgac taccgtgaag ccgtccccgc actcaaagcc
     2941 gcgtggttcg gcgatgcgga caaaatccac atcgacggct ggcagattgt cgaatgggcg
     3001 gacggttacg acatcgccgt atccgaaacg cccaaaacga aaatgccgtc tgaacacgcc
     3061 ccgcgcctgt attttgccaa tgtcggcggt tatcgcgcag ggcagctcgc cgaagcgcac
     3121 gcttttgggc tgttcgccgc cgccacgcct gccgaagcca aacaaaaagc cctgcaaacc
     3181 ctgttgaccg acagctatgt tcagcaacat aaagacaact taaaagacgt ggacaacctg
     3241 cttgcgctcg accgcatcgg caatttccat atccgcctga ccccgaatcc gcacggcaaa
     3301 cccgccgaaa tcggctttca aggctatttg cccatttgag aacccatgaa aatcaccaaa
     3361 atcttcacct tcgactcctc gcatatgctc gacgggcatg acggcaaatg ccaaaacctg
     3421 cacgggcata cctacaaact cgaaatcacc gtttcagacg accccgtcag aggcgggccg
     3481 aaagacggta tggtgatgga ctttaccgac ttgaaagcca ttgtcaaaca acacattacc
     3541 gaccccttcg accacgcctt catctaccac ggcggcaacg ggcgcgaatc tcaaatcgcc
     3601 gcactcttgg aaggctggaa catgaaaacc ctgcgcctgc cctgccgcac cactgccgaa
     3661 aatatggcgg tcgaaatgta cgaccgtctg aaaaacgcgg ggctgaacgt gtgccgcgtg
     3721 aaattgtggg aaacgccgac atcgtgtgcg gagtatgaag gggagtaggg aatatcttga
     3781 acgtatcgat atagtaaatt ccaataagac atgcccaacc gcgtcattcc cgcgcaggcg
     3841 ggaatccaga ccttgattta tcaggaatat ttaaaaattg cagcaattcc aaatctctgg
     3901 attcccgcct gcgcggaaag gacggtttag agcgtcctta tttgaattta ccgtaaaacg
     3961 gttttttctc ctgtacggat tccccgtttt ttcagacgac cttccatatc aaatacaccc
     4021 attaaaagga atatccatga aactcctctt catcccccta gtcctcttcg tcgccgtcga
     4081 acatttctac atcgcctggc ttgaaatgac gcagattccc agcgaaaaag cggcggaaac
     4141 gttcaagctg ccttatgaat ttatggaaca aaatcgcgtg cagaccctgt tcggcaacca
     4201 agggctgtat aacggctttc tcggcatcgg gctggtgtgg tcgcggtttg ccgctccgga
     4261 taacgcggtg tacggcgcaa cggtactgtt tctcggcttc gtcctgattg ccgccgcgtg
     4321 gggcgcgttc tcttccggca acaaaggcat actcgtcaaa caaggtttgc ccgcattttt
     4381 ggcagcggcg gcggtgttgg cggtatgaaa aaaatcagcg tcgcccccga aaatccccaa
     4441 taccgcatcg tcgaaatctt tgaaagcctg caaggcgaag gctggaacac gggcatgccc
     4501 gccgttttcg tccgcttggg caaatgcaac ctcgcctgcg gctggtgtga taccgattat
     4561 ttgacattcg gcatgatgag cctgtccgac atcttaggcc gtctgaaagc ctatgccgcc
     4621 cgcaacatca tcatcaccgg cggcgaaccg accatccagc cgcatctcga tatgctgctg
     4681 gacatgctca aggcggaagg ttatttcctc tgtatcgaaa ccaacggact caatcccgcg
     4741 ccgccgcaaa tcgactatgt cgccaccagc cccaaagcct gctatgccgc caaatacgaa
     4801 aaaagctgta tcgaaacagc cgacgaagtg cggattgttg ccgacggcga tgtcgttgcg
     4861 ttctgtgaaa atatggaacg caaaatccgc gcgcatcatt actacctttc gccctgcgaa
     4921 caagacggtg cgatgaacat ctacgacacc atccgccaaa tcggtatttt aaacagtcgc
     4981 cccgacgcgc cggtgcattg gcagttgagc gtgcagacgc acaaatgggc gggaatagag
     5041 tagtttaagc agtgtaactc aaagggacgg cgtacggttt taccgatgtt tgacatatgg
     5101 gaaaagtgtg ccgcttccgc gtgaaactgc ccacatttcc gctgcctgat tgaggcggag
     5161 ccttactgaa taagatgccg ccgttgggta caagcccggc ttcctaaatt ccgatggtct
     5221 tttgaacctt gccgatactc tgtgccagtg cgcgcaaatg gcagggttag ggaaaacgaa
     5281 atgccgtctg aaacaacatt ctgagacctt tgcaaaaata gtctgttaac gaaatttgac
     5341 gcataaaaat g
//
LOCUS       DAWKOD010000140         5258 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000140 DAWKOD010000000
VERSION     DAWKOD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.140"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..807
                     /gene="pgl"
                     /locus_tag="WJK73_001706"
     CDS             112..807
                     /gene="pgl"
                     /locus_tag="WJK73_001706"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ7558151.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            788..1774
                     /locus_tag="WJK73_001707"
     CDS             788..1774
                     /locus_tag="WJK73_001707"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ7558152.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1824..2672
                     /gene="hexR"
                     /locus_tag="WJK73_001708"
     CDS             1824..2672
                     /gene="hexR"
                     /locus_tag="WJK73_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ7558153.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2793..4439
                     /gene="pgi"
                     /locus_tag="WJK73_001709"
     CDS             2793..4439
                     /gene="pgi"
                     /locus_tag="WJK73_001709"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7558154.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4453..5004
                     /locus_tag="WJK73_001710"
     CDS             4453..5004
                     /locus_tag="WJK73_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7558155.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1336 a         1465 c         1392 g         1065 t
ORIGIN      
        1 aagcagtccc gatatttggt atcaaaagca taaacaactg ttcggccgac atattgctca
       61 acccgtttcc ataacggaat atgccgtctg aaataaaaaa aggacacaaa tatgtttgtt
      121 tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt tggctgacgc agtggcggat
      181 gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt tggcagtttc cggcggacgt
      241 tcgccgattg catttttcaa cgccctgtcg caaaaagatt tggattggaa aaacgtcggc
      301 atcaccttgg cagatgaacg catcgtgccg accgtccacg ccgacagcaa taccggtttg
      361 gtgcgcgaat acctgttgaa gaacaaagcg gaagcggcaa tgtggattcc tatggtggaa
      421 gacggaaaaa ctgaaaccga attacatccc gatgctgttg tcgattatgc actgaaacat
      481 tacaaacagc ccgatgtttt ggttttgggt atgggaaacg acgggcatac ggcttcgatt
      541 ttcccgaaag ctccgcagtt tcagacggca atcgacggtt cggcaggtgt cgcgctggtg
      601 cataccacgc ccgttaccgc gccgcacgag cgcgtcagta tgaccttgga tgcgattgcc
      661 catacggggc atgtgttttt ggcgatacgg ggcgaagaga aaaaagccgt gttcgaccaa
      721 gccgcacaag gcgaaaaccg cgaatatccg atcaacctcg ttttgaacca tcaaggagtg
      781 aactgccatg tcttctacgc cgaataaaca agccggatat ccccgactgg tcgccgatat
      841 cggcgggacg aatgcacgct ttgcgctgga aaccgcgccg cgcgtcattg aaaaagccgc
      901 cgtgcttccg tgtaaagact acgatacggt taccgatgcg gtgcgtgcct atctgaatca
      961 aagcggtgca acagccgtac ggcacgcggc atttgccatc gccaacccga ttttgggcga
     1021 ctgggtgcag atgaccaacc accattgggc gttttccatc gaaaccaccc gtcagacttt
     1081 ggggctggac accctcatcc ttttgaacga ctttaccgcg caggcattgg cggtaacgca
     1141 gacttcaagc aaagacctga tgcaggtagg cgggcaaaag cctgtcgaat ttgcccccaa
     1201 agccgttatc ggccccggta ccggcctggg cgtgagcgga ttggtgcaca gccacgcagg
     1261 ctgggtggct ttggcgggcg agggcgggca taccagtttc ccgccgtttg acgatatgga
     1321 agtgctgatt tggcagtacg ccaaaaacaa atacggccat gtttccgccg aacgcttttt
     1381 gagcggcgcg ggcttgagct tggtttacga ggctttggct gcaaaacaga aagccaaacc
     1441 cgccaaactg atgccgtctg aaatcacgga aaaggctttg agcggcgcgt cgcctttgtg
     1501 ccgtcagact ttggacatct tctgcgccat gctcggcacg gttgcttcca acctcgccct
     1561 gacgctgggc gcgcgcggcg gcgtgtacct gtgcggcggc attattcccc gcgtgttgga
     1621 atatttcaaa acttccccgt tccgcagccg tttcgagaac aagggcaggt ttgaagcata
     1681 tcttgccgcg attcccgtgt atgtcgtctt gagcgagttt cccggaattt ccggtgcggc
     1741 tgcggctctc gacaaccatt tgagaaacgt ttaaccacag cggctccttg cagcggggct
     1801 gcattatcga agggcatatc attatgttaa gcaaaatcag cgaatcactg gcaaaccttt
     1861 ccggtgcgga acgcaaagtc gccgaatgtg cattggcgga acccaaatgg ttcgtccatg
     1921 ccgccgttgc cgaaattgcc gaacgtgcgt ccgtcagcca gccgaccgtc atccgattct
     1981 gccgcagctt gggttataaa gggctgcccg agttcaagct cgccttgtcc gccagcatcg
     2041 gtcatgaggg tatgccctat gtccacgaag aactcaacgc cgacgacgat atggcaagcg
     2101 tggtcgagaa agtgttgggc aatgccgccg cctcgctgtt gggcgaacgc cgcttcctga
     2161 aagagtcgga gctggaaaac gccattgcca cgctgatgca cgcccgtcgc gtcgagtttt
     2221 acggtgtcgg caattccggc attgtggcac aggacgcgca gcataaattt ttccgtttcg
     2281 gcatgtccac cgtcgcctat gtcgatacgc acacgcagct gatggcggca tctgttttga
     2341 gcgatcagga tgttttggtt gccatttcca acacgggttc gtctatcgaa cttttggatg
     2401 cggtcagcat cgccaaagaa aacggcgcgt ctgtcatcgc actgacccgc aacgattcgc
     2461 ctctggcgca acttgccgac tgcgtgttga gcgttgccac acaggaaaat gccgaactct
     2521 acacgcccat ggtttcccgc ctcttgcagc ttgccgtcat cgacattctc gccatcggac
     2581 ttgccctgcg cttgggcgat gctgccagcc tgcaattgca gaaaagcaaa aaaagcatac
     2641 acaacaagca catcgattac gacaaagatt gaccttcaga cggcatccca caaatgccgt
     2701 ctgaaatgcc gaacaacggt cgtcggcggc ttgcggcagt ttccggcagc cttttcatcc
     2761 cacaacaaaa aacctcattc aggagcatat agatgaaaca ccttcacgac ttacccgcat
     2821 ggtcgaaatt gtggaaccac tttgacgaca gcaaaacatt gcatatgcgc gaaatgttcg
     2881 agcaagaccc gcagcgtgcg gaacgctact ggctgcaagt cggcggactg acgctggact
     2941 actccaaaaa ccgcatcaac gacgaaacca tgtcgctttt gttcgagctt gcccgagaag
     3001 caggcgtgcc ggagcggatg cggcagatgt tccacggcga aaaaatcaat accaccgaaa
     3061 accgcgccgt cctgcatgtc gcccttcgca accgcaccaa ttcgccgatt gtggttgacg
     3121 gtgaagatgt gatgcccaaa gtcaaccgcg ttttgcaacg tatgggcgaa tttgcacacg
     3181 aagtccgcag cggaagctgg ctgggctata ccaaccaagt cattaccgac gttgtcaaca
     3241 tcggcatcgg cggatcggat ttgggtccgc tgatgatgtg taccgcgctc aaacctttcg
     3301 gtcatccgcg cctcaatatg cacttcgtct ccaacgtgga cggctcgcaa ctgcgcgacg
     3361 tattgtccaa agtccacccc gaaaccacgt tgttcatcat cgcctccaaa acatttacca
     3421 cgcaggaaac gctgaccaac gcgctgaccg cgcgcgaatg gtttttgaat catgcgggcg
     3481 acgaagaagc cgttgccaaa cacttcgccg ccgtttccac caatcaaaaa gccgtcgccg
     3541 aattcggcat cgacaccgcc aatatgtttg aattttggga ttgggtcggc ggtcggtaca
     3601 gcctgtggtc cgccatcgga ttgccgatta tgctgtatct cggcgaagaa aacttcatcg
     3661 aaatgctcaa cggcgcgcac ctgatggacc aacacttcat caacacaccg ctcgagcgca
     3721 acctgcccgt cattctcgcc ctcatcggca tctggtatat caactactac ggcggcggca
     3781 gccacgtcat cgcgccttac gaccaacatt tgcaccgcct gcccaaattc atccagcagc
     3841 tcgatatgga aagcaacggc aaacaggtta cgttggacgg caaagcagtc ggacacgaaa
     3901 cctcgccgat tatctggggc gaaacgggca ttaacggcca gcacgccttc ttccaactgc
     3961 tgcaccaagg cacgcacatt acccccatcg acctgattgc ctcgcttgaa aaacgcagca
     4021 acctgcccgg acaccacgaa atcctgcttg ccaatgtctt cgcccaagca gaagccttta
     4081 tgcgcggcaa aacccccgac gaagtccgcg ccgaactcaa agcgcagggt atggatgagg
     4141 cgcgcatcga agagctggtc ccgcacaaaa ccttctccgg caaccgcccg accaacctca
     4201 ttctcatgga caaggtcaac ccgcgcaata tgggcagcct gattgccatg tacgaacaca
     4261 aaaccttcgt acaaggcatc atttggggca tcaacagctt cgaccagtgg ggcgtggaac
     4321 tcggcaaaca actggctaaa accattttgg gcgaactgac cggcgaaacc gggccgcaaa
     4381 agcacgacag ttcgaccgaa cgcctgatca acctctacct gcagaccaac cgcaaataaa
     4441 acctgcggaa aaatgccgtc tgaacgccga ccgtttcaga cggcattttt atcgaacagg
     4501 aaaaccgtcg gtaaacttgc agagcgtgtg caatcccgat atgatggttt gcataaattt
     4561 aaacatatat gttccgcagc tatggcactg attaaagagc cgttggacaa agcgaaacaa
     4621 aggaacgaag aacttgaagc ggcagaagaa gcggcggcgc aggaggcatt gggtcgggag
     4681 caggaagtcg accgcgtatc cgaatgggaa gaacgctaca agctgtcgcg cagcgagttc
     4741 gagcagttct ggaaaggatt gcctcaaacc gtacagaata agctgcaagc ctcacagaaa
     4801 acatggaaaa gcgggatgga taaaatctgt gccaacaatg cgaaagctga aggtaaaacg
     4861 ccaaacggca taaaattcag cgaactggca tgcaaaacgg cgaaaaccga agcacgcttg
     4921 gaagagctgc acaaccgtaa aaaagccctt atcgacgaaa tggccaggga agcggacaag
     4981 aaagaactgt caaagcggct ctgaacagcg cggttcaggc actgcccgcc gatattgccg
     5041 aaaccgtcat gcccgagtac cgcaactgac aaaacggttt gaatgccaaa tgcgccgacg
     5101 gcgacgaata cggcgtggca caatctgact gccgtaccag agaaatcaat gcgaaaacca
     5161 aagaaatcca aggttatctg attgactgaa acttggatgc gggaatgtcg gcggcttttg
     5221 cgtttttgtc gtttttatag tgaattaaat ttaaaccg
//
LOCUS       DAWKOD010000141         5256 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000141 DAWKOD010000000
VERSION     DAWKOD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.141"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..169)
                     /locus_tag="WJK73_001711"
     CDS             complement(<1..169)
                     /locus_tag="WJK73_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HEZ7558156.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYE"
     gene            272..487
                     /locus_tag="WJK73_001712"
                     /pseudo
     CDS             272..487
                     /locus_tag="WJK73_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(639..1697)
                     /gene="trpD"
                     /locus_tag="WJK73_001713"
     CDS             complement(639..1697)
                     /gene="trpD"
                     /locus_tag="WJK73_001713"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ7558157.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1761..2351)
                     /locus_tag="WJK73_001714"
     CDS             complement(1761..2351)
                     /locus_tag="WJK73_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ7558158.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2771..5047
                     /locus_tag="WJK73_001715"
     CDS             2771..5047
                     /locus_tag="WJK73_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7558159.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1349 a         1481 c         1202 g         1224 t
ORIGIN      
        1 tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt ttcggtttct gtacccctcc
       61 tgttagataa ggggggaaga tttgaagcgg ttgccggctt cctgccgtcc gctaacgcgt
      121 ccgtcatcac gccgacaacc gcctttgtca tcccttgctt atcttccatg gtgcgaatcc
      181 tcaaaaacag gcaaaaaaaa agccctgtta cttgtagaaa gtaaaggacg ttaatttttg
      241 ttaatcgtcc cttcttaggg acgcaatata taaggtttat accgtcgttg ttcctaatgc
      301 gcaatcagcg acattgttgc caattatccg aaagaaagtt aagcctgatg gcattgtata
      361 cacggatacc tttcgtagtt atgatgtgct tgatattagt gaatttagcc atttacgtaa
      421 gtttaacggc attcccaaag agcatttggg gctgcattta aagaaatgcc aatggcattt
      481 taaatagaat ctcaaattca gattttaaga caattagtta atgggaattt ggtctggtta
      541 tctagtacag ccccaagttt ttatataaaa acagtttggt aagttctctt cgtgaagtgc
      601 ttatgttttg ccacaaaagt tccataatca tggcggcatc acccaagctc catctttcca
      661 agaaaatggc atctttgttg tggttgaaaa ccgacaaact cctctttttt cgatttggcc
      721 ctgcctgaat cgatggcttc ccgtgcggca gatatgccgt ctgaaagcga agcagcgaca
      781 tttccggcat atagggcggc ggcggtatta agcaatacga tatcgcgcgc agccccttct
      841 ttcccatcca gcacctcatt cattttcaac aaagattcct gagtattggc aactttgatt
      901 tcatccaaat tgcggcgggt ttcgataccg aaatcttctg ggcggatgtc gtattcgctg
      961 atttttccgt ctttgagttc ggcaacgcgc gttttgcccg tcagtgtaat ttcatccaaa
     1021 ccgccctccc cgcaaacgac taaaacgtgt tttgaaccga gttgttgcaa gacccgcgac
     1081 aaaatgccgc acaaatcggt gtggaacacg cccaaaagct ggttcggcgc gcccgcagga
     1141 ttggtcaacg gacccaatat gttgaaaatg cttcggaaac cgagcgaacg gcgtaccggg
     1201 gcgacatggc gcatggcact gtggtgattg ggggcgaaca taaaccctat accggtctgg
     1261 ctgatacttt gggcaatctg ttcgggagtc aggttgaggt ttgcgcccat ctgctccatc
     1321 acgtccgccg cgccgctgga agatgaaacg gaacgcccgc cgtgtttggc aaccttcgcg
     1381 cctgccgctg cggcaacaaa catcgaagtc gtcgaaatat tgaaggtttt ggcaccatcc
     1441 ccgcccgtac cgacgatatc aaccagcccc tccgcattct ccagcggcac ttttgtcgca
     1501 aactcgcgca tgacggctgc agctgcggta atttcggaaa cggtttcaac cttgatacgc
     1561 aatcctgtca aaatcgccgc aatttgttcg ggcggcactt ttccgctcat catttggcgc
     1621 atcaagtcgg tcatttcatc gtaaaacaac tcattattgc tgattaatcg ttcgatagcc
     1681 tgttgcggtg taatcatttt ttgtcctccg ttcaatattc ggacgaaaat gccgtctgaa
     1741 gggcttcaga cggcatcacg tcagattttt tgcggtttga agttttgaaa ttcgattaaa
     1801 aaattgttta acatatcatg tccgtgctcg gtcaagaggg cttcggggtg gaactgcacg
     1861 ccctcgacgg catattcctt atggcgcaca cccataatct cgccgtcctc agtccaagcc
     1921 gttacttcca aacattcggg catcgtattc cgatcgataa cgaggctgtg ataacgcgta
     1981 caggtaaccg gattgggcaa acccttaaac atacccttgc ccgaatggga cacgggcgac
     2041 accttaccgt gcatcagcgt tttggcgcgg actatcctgc cgccgaacgc ctcgcctatc
     2101 gtctgatgcc cgaggcacac gcccataatc ggcagccggc cggcaaaatg gcgcatcgcc
     2161 gccacggaaa tccccgcttc tttgggcgaa cacgggccgg gtccgataac gagatattgc
     2221 ggattcaatg cctcgatttc ttccaacgta atatcatcgt tgcggcgcac ggcgacttcc
     2281 tgccccaatt cagcgaaata ctggacgatg ttgtaagtaa aactgtcgta attgtcgata
     2341 aacaaaagca ttttgtgatt aacctattga tttataattt gtttgttatt aactgcattt
     2401 ccgataccac catccatacc ccttatatct tagcgtgccc gatgcgccct cgtgaacctg
     2461 ggcagagcct cgcgttctga caagtacgcc attttgccac cttttcgacc gttcgggaac
     2521 gtgccgtttt tatgcactca taaattttgg ttatcccgca gccgaacata gtgggcaaac
     2581 acagcggaat ctgcgtcaac atcaaccgcg cccccgaatg acggcggata caaatggaca
     2641 ccgcccgatg ccgcaaccgt ttcatttgaa aaacttttgc ctatgccctc tgagtgttct
     2701 caaaatctta tgatataaaa aatgtaatgt tatataataa caaacttttg aaatgcgacg
     2761 agaagccaaa atggcacaaa ctacactcaa acccattgtt ttatcaattc ttttaatcag
     2821 cacccccatc ctcgcccaag cgcatgaaac cgagcagtct gttgacttgg acgaggttac
     2881 cgtcgtcggc aaaagccgtc cgcgcgccac atccgggctg ttgcacactt cgaccgcttc
     2941 cgacaaaatc ctctccggcg ataccttgcg ccaaaaagcc gtcaacttgg gcgatgcttt
     3001 ggacggcgtg ccgggcattc acgcctcgca atacggcggc ggcgcgtccg ctcccgttat
     3061 tcgcggtcaa acaggcaggc ggattaaagt gttgaaccat cacggcgaaa cgggcgatat
     3121 ggcggatttt tcgcccgatc acgccattat ggtagatacc gccttgtcgc aacaggtcga
     3181 aatcctgcgc gggccggtta cgctcttgta cagctcgggc aatgtggcgg ggctggtcga
     3241 tgttgccgat ggcaaaatcc ccgaaaaaat gcctgaaaac ggcgtatcgg gcgaactcgg
     3301 attgcgtttg agcagcggca atctggaaaa actcacgtcc ggcggcatca atatcggttt
     3361 gggcaaaaac tttgtattgc acacggaagg gctgtaccgc aaatcggggg attacgccgt
     3421 accgcgttac cgcaatctga aacgcctgcc cgacagccac gccgattcgc aaacgggcag
     3481 catcgggctg tcttgggttg gcgaaaaagg ttttatcggc gtagcgtaca gcgaccgtcg
     3541 cgaccaatat ggtctgcctg cccacagcca cgaatacgat gattgccacg ccgacatcat
     3601 ctggcaaaag agcttgatta acaaacgcta tttacagctt tatccgcacc tgttgaccga
     3661 agaagacatc gattacgaca atccgggctt gagctgcggc ttccacgacg acgatgatgc
     3721 acacgcacac acccacagcg gcagaccgtg gatagacctg cgcaacaaac gctacgaact
     3781 ccgtgccgaa tggaagcaac cgttccccgg ttttgaagcc ctgcgcgtac acctgaaccg
     3841 caacgactac cgccacgacg aaaaagcagg cgatgcagtc gaaaactttt ttaacaacca
     3901 aacgcaaaac gcccgcatcg agttgcgcca ccaacccata ggtcgtctga aaggcagctg
     3961 gggcgtgcaa tatttacaac aaaaatccag tgctttatct gccatatccg aagcggttaa
     4021 acaaccgatg ctgcttgaca acaaagtgca acattacagc tttttcggtg tagaacaggc
     4081 aaactgggac aacttcacgc ttgaaggcgg cgtacgcgtg gaaaaacaaa aagcctccat
     4141 tcagtacgac aaagcattga ttgatcggga aaactactac aaccaccccc tgcccgacct
     4201 cggcgcgcac cgccaaaccg cccgctcatt cgcactttcg ggcaactggt atttcacgcc
     4261 acaacacaaa ctcagcctga ccgcctccca tcaggaacgc ctgccgtcaa cgcaagagct
     4321 gtacgcacac ggcaaacacg tcgccaccaa cacctttgaa gtcggcaaca aacacctcaa
     4381 caaagagcgt tccaacaata tcgaactcgc gctgggctac gaaggcgacc gctggcaata
     4441 caatctggca ctctaccgca accgcttcgg taactacatt tacgcccaaa ccttaaacga
     4501 cggacgcggc cccaaatcca tcgaagacga cagcgaaatg aagctcgtgc gctacaacca
     4561 atccggcgcc gacttctacg gcgcggaagg cgaaatctac ttcaaaccga caccgcgcta
     4621 ccgcatcggc gtttccggcg actatgtacg aggccgtctg aaaaacctgc cttccctacc
     4681 cggcagagaa gatgcctacg gcaaccgtcc tttcatcgca caggacgacc aaaatgcccc
     4741 ccgtgttccg gctgcgcgcc tcggcttcca cctgaaagcc tcgctgaccg accgtatcga
     4801 tgccaatttg gactactacc gcgtgttcgc ccaaaacaaa ctcgcccgct acgaaacgcg
     4861 cacgcccgga caccatatgc tcaacctcgg cgcaaactac cgccgcaata cgcgctatgg
     4921 cgagtggaat tggtacgtca aagccgacaa cctgctcaac caatccgttt acgcccacag
     4981 cagctttctc tctgatacgc cgcaaatggg ccgcagcttt accggcggcg tgaacgtgaa
     5041 gttttaaaat cggacaggca aacaaaaaag gttttcagac ggcattctga aaaccttttt
     5101 tctttttaat caatttgtta ggaaatatgg caaagacaaa gaacaaacca ctatgccatc
     5161 attcccattt tttttgtttc atcattctta ctttcacgcc gtcattctcg caaaagcaga
     5221 aaaatcaaaa gcagaaaccc aagatcccgt cattcc
//
LOCUS       DAWKOD010000142         5207 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000142 DAWKOD010000000
VERSION     DAWKOD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.142"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..1445)
                     /locus_tag="WJK73_001716"
     CDS             complement(90..1445)
                     /locus_tag="WJK73_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ7558160.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="WJK73_001717"
     CDS             complement(1460..1732)
                     /locus_tag="WJK73_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ7558161.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1857..2594)
                     /locus_tag="WJK73_001718"
     CDS             complement(1857..2594)
                     /locus_tag="WJK73_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ7558162.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WJK73_001719"
     CDS             complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WJK73_001719"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7558163.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3919..4512
                     /locus_tag="WJK73_001720"
     CDS             3919..4512
                     /locus_tag="WJK73_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558164.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNKSLNIGVIVREFPDDHKHYDHFILKDE"
     gene            4624..>5207
                     /locus_tag="WJK73_001721"
     CDS             4624..>5207
                     /locus_tag="WJK73_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ7558165.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAATAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLSAGRAGFA"
BASE COUNT         1430 a         1333 c         1150 g         1294 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggtgcggga
      121 atcctgccgc cccggttgac gaacacttcg catgagcctt cttttaccgg cattacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggttttacc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggtgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc ttcggcggcg
      421 gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgcg taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaggc ggttttcttc
      721 acaacttcgg caacttcggt caatgtcgtt gcatccgaat tttccaacgc ggcttttacg
      781 acacccgggc cggatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacgggtt gtcttccacc gcgttgcaga acacgacgat tttggcgcag
      901 ccgaaacctt ctagtgtgat ttcagccgtg cgtttgatgg tttcgcccgc cagtctgacc
      961 gcgtccatat tgataccggc gcgcgtactg ccgatattga tggagctgca cacaatatcg
     1021 gtggtcttca tcgcttcggg aatggagcgg attaacacct catccgaagg cgacatgcct
     1081 ttttgcacca acgcggaaaa gccgccgata aaggacacgc cgatggcttt ggctgccttg
     1141 tccaaagtct gcgccacgct gacataggaa tcggctttag ttgccgccgc gatttgggca
     1201 atcggcgtga cagaaatgcg ctgattcaca atcggcacgc cgtatttggc ggaaaggtgt
     1261 ttcgccgttg ccaccaagtc tttgccgact gtggtgattt tgttgtaaat attttggttt
     1321 aacacgtcga tgtcgctgct gatgcagtcg tgcaaatcga tgcctatggt aatggtgcgg
     1381 acatcaaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc ggattggata
     1441 ctcatcacat tcctccgact caaatgcggt gcatcgcttg gaagatttct tcattttgca
     1501 tacggatatc aagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcctgcc
     1561 gagatttggg gcatttcgag gtatccacca agataatcat ggtaaaaaaa tcatccatca
     1621 gctgttggct gatattaagg atgttgattc ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccacca tcggattttg aggaaatatt
     1801 gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacggca tcaaatttag
     1861 ggttcgatta aataaccatc cttatcccac tgggttttcc taaccaactt atcatcctga
     1921 taaacagctt cactcttttt agaaccatct tcataccact ctaagactac tccattacgt
     1981 ttatgatgat ggatagacag ttccgagagt aatcgaccgc tttcatccca tgtcagaatc
     2041 ttggcaggct catcattgac cataaccatt tccgttttga tattgccgtc ggcataccat
     2101 tgcttccata cgccgtttgc cttattctgt ttaaactgga tttcgctttc cttgccgccg
     2161 ttacggtaat agcggtaccc cgtaccttca ctcaaaccat ttttataagg cataacggca
     2221 gattttttac cgttcggata ccagttgacc cactccccgt ccggcttacc cttgctgaag
     2281 ccccccgcca tttttttctg accattaaaa tgccacaaaa tcaacatacc gttttgcagg
     2341 gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat aaggttcgga atatttcttc
     2401 atcgacggat aataaaaatc ctgcgcgtgc gcaataccct ccgccacact atattgcctg
     2461 atataagcgg cagaagacat cgtcgccgtc agtttcccgt tctgattaaa ataaacagaa
     2521 taggtctgcg ccggcaaagc ggccgaaaaa cccaacagga cagttgaaaa tacaatccga
     2581 gataattttt tcattgcaat agcgatataa aaacaaggct gtgttttagt aatcttttga
     2641 tttcaattat ttgcaaggga aaagacaatt attttccggt taggaataaa cctattctat
     2701 tgaatatatt gaagccaagt acgcctatca acactatatt aaaacactgc caaaaacaat
     2761 taacttataa acaatatggt aaggatttct ctgccaagca tcaaacccga gacaacgtat
     2821 cgtaaaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttcccctt caactcactc
     2881 ttcacccaat aactgctcgc gcgtcaagag gaaaacaaaa ccgtcgcccc cgctggtttc
     2941 caaccaagta aaaggcaact ccggatacgc tgcttccaat acatccctgt tatgcccgat
     3001 ttccaccagc aatacacctt tgggattcag aaactttgcc gcattcagaa gaatctgcct
     3061 ggtggcatcc aacccgtccg ccccgctgcc caatgccaat tccggttcgt gcaaatactc
     3121 ttcaggcaat aactcaaccg attccgcatc cacataaggc ggattggaaa caatcaaatc
     3181 ataagtgcct tccaatcctt caaacaaatc cgtatgaata agccggatgc gttcttccaa
     3241 accataatct tcgacattaa tccctgccac ttccaaagca tccaagctca catcaaccgc
     3301 atcaatttgg gcatcaggat aatgatgcgc catctgaatg gcaaggcaac cgcttccggt
     3361 gcaaagatcc aaagcattat gcaccagctc atcgtattct atccaaggac gaagtccgtt
     3421 acccaacaat tcataaataa aagaacgagg tatgattacg cgctcatcca catagaaatc
     3481 aaactctccc tgccatgcct ggtgtgtcaa ataagcggct ggaatgtgtt cgacagcacg
     3541 acgctcaata accgccagca cttcctcttt ttcagcttcc aagagttttg catcaagata
     3601 tggggcaagc atatccaaag gcaaattcaa agtatgcaga atcaaataag ccgcttcatc
     3661 atgcgcatta tctgttccat gaccaaaaaa gagccctgcc tcattaaaac ggctgactgc
     3721 aaaacgtaaa atatcgcgga tagtcgtcaa ttcttgtgct gcctgattaa acataatatg
     3781 aaccattctg cgtatagata cttttaatta taacagaaac aacaagcaaa ccttttcata
     3841 tcgccaaata accacccaat ctacccatac aactacataa atgcccgcgc gaaaaccatc
     3901 gcccgaacgg aaacgacaat ggccgacggt atgggcaatc tgattggctg ggaaaaaacg
     3961 gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc cgatgtctgc
     4021 aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca cggcgcattg
     4081 acgatacccg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg tggcgaattg
     4141 ggggaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc gcgggataac
     4201 tttatcggca aaagctatgt caatgaaaac agcgggcatg aactgaaggt aacttggcaa
     4261 ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat gacaaaactt
     4321 gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataggaa aggtcatcct
     4381 aatattattg cagcacataa gtatcgtgcc gttgccaagg ttgggaataa gtctttaaat
     4441 atcggtgtga ttgtaaggga atttccagac gaccataaac attacgacca tttcatcttg
     4501 aaggatgaat aaagcccttt tgcagtgtcg ttctggagcg gatagcgtta aggcaagtac
     4561 acttccagcc ttgaaaaagg gctttaaatt cagcatgcca tttatacagg caggagtaaa
     4621 cccatgacaa agttatacgc agaaatcgcc aagatggaga cgcaggacga cgacacggtc
     4681 aaggtttggg gttacgcttc aagcgaggaa atcgattcgg acggcgaagt catcgcggcg
     4741 acagctatga aggcggcgat tcccgattat atgaagtttg gcgcggggcg cgagatgcac
     4801 ggctcaaacg ctgcgggaac ggcaattgaa atcaacgttg aagacgacgg cataaccttt
     4861 ttcggtgctc atatcatcga ccccgttgtc gtgtcgaaag tcaaaacagg cgtttacaaa
     4921 ggcttttcca tcggcggcag cgttaccgcc cgcgatgatt tgaacaagtc gcaaatcacg
     4981 ggcttgaagc tgacggaaat cagccttgtc gagcgccctg ccaatcccga tgcggtgtct
     5041 acctgcttta aggcggacaa aggtgcggaa acggtaaaca acgatacaga acataatgct
     5101 acatatttta gccatttccc ttccaaacaa aaaagcaccg acggcggccg atgccccttc
     5161 ctttacaggt tcccctattt tttatccgcg ggcagagccg gttttgc
//
LOCUS       DAWKOD010000143         5123 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000143 DAWKOD010000000
VERSION     DAWKOD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.143"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(148..1191)
                     /locus_tag="WJK73_001722"
     CDS             complement(148..1191)
                     /locus_tag="WJK73_001722"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ7558166.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1188..1481)
                     /locus_tag="WJK73_001723"
     CDS             complement(1188..1481)
                     /locus_tag="WJK73_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558167.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            1849..2169
                     /locus_tag="WJK73_001724"
     CDS             1849..2169
                     /locus_tag="WJK73_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ7558168.1"
                     /translation="MSIYLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLLYNH
                     IIVD"
     gene            2188..2997
                     /locus_tag="WJK73_001725"
     CDS             2188..2997
                     /locus_tag="WJK73_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7558169.1"
                     /translation="MPRLAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQ
                     LTAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHL
                     AVGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYE
                     AFSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKIS
                     VDELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            3226..3579
                     /locus_tag="WJK73_001726"
     CDS             3226..3579
                     /locus_tag="WJK73_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558170.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDFVAI
                     PAAAATVHFLPKNFFGAIGANGVYNLTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3640..4356
                     /locus_tag="WJK73_001727"
     CDS             3640..4356
                     /locus_tag="WJK73_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ7558171.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGDSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4421..4915
                     /locus_tag="WJK73_001728"
     CDS             4421..4915
                     /locus_tag="WJK73_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7558172.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
BASE COUNT         1387 a         1165 c         1282 g         1289 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacggtattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc cgaacccaaa aaaaccgtcg ctttcagacg gcatcaaaaa accgcaggca
      121 cgatgcctgc ggtttgccgt ttgcgcttca ggggagcagg gggattgcgc ccaagccgtg
      181 tgtttccttc agtccgaaca tgatgttcat attttgcacc gcctgacccg ccgcgccttt
      241 gacgaggttg tcgatgacgg aaaggacgac ccacacatcg gattgcgccg cctgttggat
      301 gctgatgcgg cagaggtttg cgccgcgcac gctgcgggtt tcgggtgtcg aaccggcggg
      361 caggatgtcc acgaacaggc tgtcgcggta atagtcgcgc aggatggttt cggggcagat
      421 gccgtctgaa aggtggaggt aaacggtggc gtgcataccg cgtatcatcg gcgcgaggtg
      481 cggcgtgaac acgaatcctt cggcgatgcc gtcctgaagc ccggcgatgg tctgcctgat
      541 ttcgggcagg tggcggtgtc cagctatgcc gtatgctttg aagttgtcgc cgacttcgca
      601 caacagcgaa ccgacattgc ctttcctgcc cgcgccggac acgccggatt tgcagtcggc
      661 aatcagcggc ataccgggct tcagacggca ttgccgcaac agcggcacga gcggcaggga
      721 tacgcaggtc gggtagcagc cggggttggc gacgaggcgt gcctgtgcga cggcttcgcg
      781 gttgagttcg ctcaatccgt acacggcttg ggaaacgagg tcgggggcgg cgtgggtcat
      841 gccgtaccag tgttcccagg tcggaatgtc ccgtatgcgg aagtcggcgg aaaggtcgat
      901 gacgcgcacg ccctgttcaa tcaggcgcgg cgcgtctttc atggcgatgc cgttgggcgt
      961 ggcgaagaag acgatgtcgc attgttccaa acctgcctcg tcgggcgttt ggaaggcgag
     1021 gccgtacacg ccgcgcaaac tcggaaagta atcggcaact gcagttcccg cttcgctgcg
     1081 gctggttacg gcggcgactt cgacatcggg atgggcggca agcaggcgca gcagttccac
     1141 gcccgtgtag cccgtcgcgc cgacaatgcc gactttgatt tttttactca tggtttttcc
     1201 tttgtgtggt tggcgggtat gccgtccgaa cgctgtccga cggcttgtcc gacggggttt
     1261 tgattggcgg cggaaggacg gtacacaaag cgtttgaaaa gcgtgtctaa tttaatggca
     1321 tccgcctctg tttttaaaac ccagccctgc cgtccggaat aaacataacc gtaggcgcga
     1381 aagtcggcag gatatttcgg tgcctgcccc aaacccaaac cgtatgcggc gaaagcggtg
     1441 gtcgtggaga aggaaagcag gaggaggacg agggttttca taaggttcca aaccgaacgg
     1501 ggcgatgaag cccgattata gcaaaaggtc gtctgaaaac cgttttcaga cgacctttta
     1561 tttaattaca ataaaaatgt taacaacaaa aaacaaaccg ctttttttcg tttggaaaca
     1621 aaacaaattt ttatagtgga ttaaatttaa accagtacag cgttgcctcg ccttgccttg
     1681 ccgtactatc tgtactgtct gcggtttcgt cgccttttcc tgatttttgt taatccacta
     1741 taactaaaat tgtcaacaat tccttgacgc acacacaaaa aaaactttaa tatttgtaaa
     1801 tatatgtaac attataaatt ttaaatattt tctgagtaag gggaagtaat gagcatctat
     1861 ctcgacttcg gcattagtcc taaaacgttc cgacagactt atctgtatca aaagcccaag
     1921 ctctttaaag gagcggttcg gaatctcgaa gccgcatctt ggaaagatat caacgagata
     1981 taccaacgag cagacccaac cgcaccgctg tttcatctgc gtaaaaaagg tgcaattgtt
     2041 cctaaagaag aatacgtcga aagtttcgac gatttgggca aaactcgcta ccgttttatt
     2101 aaatccgtta tctacgaaca tatgaagaat ggtgcgtcgt tgctctataa ccatattata
     2161 gtggattaaa tttaaaccag tacagcgttg cctcgccttg ccggcgcaca tactattgtt
     2221 agtgggtatc ttgcttttgg cagcgacgaa tcttataaaa accattggga catccgagat
     2281 gtgtatgcca tccagctttt cggcaagaaa cgttggcaac ttactgcccc tgatttccct
     2341 atgccattgt atatgcaaca gactaaagat actgatattt ccattcctga acatatcgat
     2401 atggatatta tccttgaagc aggtgatgtc ctctacatcc cgcgcggttg gtggcacaga
     2461 cctatcccgc tcggctgtga aaccttccac ctcgctgtcg gtacctttcc gcccaacggc
     2521 tataattacc tcgagtggct aatgaagaaa ttccccacca tagaaagtct gcgccacagt
     2581 ttctcagact gggagcaaga taggacgcgt atcaacgata ctgccgcaca aattgctgcc
     2641 atgattgccg accccgtcaa ttacgaagcc ttcagtgaag acttcctcgg caaagaacgt
     2701 accgataccg cttttcatct cgaacagttc gcgaatccca acgctactcc gctttcagac
     2761 gacgtcaggt tgagactaaa tgccaataat ttggatacgt tggaaaaggg atatttgatt
     2821 gggaatggga tgaagataag cgtagatgag ttggggaaaa aagtgttaga acatatcggt
     2881 aagaatgaac cgttattgtt gaaaaatcta ctggttaact tcaatcaggg aaaacatgaa
     2941 gaagttagga agttgattta tcagttgata gagttagatt ttctggaact tttgtgaggg
     3001 attctatgaa aaactggaag caatttacat ttttcgtaat attagtaata gcttgttatc
     3061 aagtgctata ttttttatca gatatgtttc ttctcgacta cattaacaaa tatagttgga
     3121 atttgaattt tattcagggt actttgaatt ttttttcaat atatctgcca tatgtttttg
     3181 ttagccgaat ttttaggaat accaaccaag aaaaggagta taaaaatgat tgaacttcaa
     3241 cttcatgaat tgaagctggt ttcaggggga ggtcctgtaa cagacaatat agctggaaat
     3301 gtagctaatg ctgccacaac caaaggaggt cccacatggg gggattttgt tgcaatacct
     3361 gctgctgcag cgacagttca tttcctgccg aaaaattttt tcggtgctat tggtgcaaat
     3421 ggtgtataca atctgactcg tgattgggta aatgatgccg ttaatgcacc tccttataac
     3481 ggaagaccaa tctttgagat tgaacatgga ttaactgctc ccgcaacaaa agcagataaa
     3541 tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc tttcagacaa
     3601 tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc caagcaaccc
     3661 ttcttccgtc ccgaagtcgc cgttgcccgc caaaccagcc tgacgggtaa agtgattctg
     3721 acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc gttattgatt
     3781 atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg acaaatttta
     3841 cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac agcgaaattc
     3901 gtggaagatg gagaaaaggt taaggctggc gacaagctat ttgcgctttc gacctcacgt
     3961 ttcggcgcag gagatagcgt gcagcagcag ttgaaaacgg aggcagtttt gaagaaaacg
     4021 ttggcagaac aggaactggg tcgtctgaag ctgatacacg ggaatgaaac gcgcagcctt
     4081 aaagcaactg tcgaacgttt ggaaaaccag gaactccata tttcgcaaca gatagacggt
     4141 cagaaaaggc gcattagact tgcggaagaa atgttgcaga aatatcgttt cctatccgcc
     4201 aatgatgcag tgccaaaaca agaaatgatg aatgtcaagg cagagctttt agagcagaaa
     4261 gccaaacttg atgcctaccg ccgagaagaa gtcgggctgc ttcaggaaat ccgcacgcag
     4321 aatctgacat tggccagcct cccccaagcg gcatgagaca gaacaaagct ggcttgaacg
     4381 caccattgcc gatatttctc aagaagtttt ggattttgaa atgcgctctg aacaaatcat
     4441 ccgtgcagga cggtcgggtt atatagcaat accgaacgtc gaagtcggac agcaggttga
     4501 tccttccaaa ctgctcttga gcattgttcc cgaacgtacc gagctatatg cccatctata
     4561 tatccccagc agtgcagcag gctttatcaa gccgaaagac aaggttgtcc tacgttatca
     4621 ggcatatccc tatcaaaaat tcgggcttgc ttccggcagt gtcgtatcag tagcaaaaac
     4681 ggcactgggc agacaggaat tgtcgggatt gggcatggta tcctccgatt tggcgaagag
     4741 caacgaacct gtttatctcg tgaaaataaa acccgacaaa ccaaccatca ctgcatacgg
     4801 tgaggaaaaa ccgctgcaaa tcggcatgac gttggaagca gacatcctgc acgagaaacg
     4861 gcggctgtac gaatgggtat tggagctgat ttatagtatg tcgggcaaac tgtaagactt
     4921 gaggctgaaa gataaagaag ttgtctgaaa ccttttcaga cgaccagaag acaatatata
     4981 aaaacacaaa cttcaaaact gcctgaaacc atcccctttt acaggcactc cgtctacagg
     5041 cactccgttt gtaaaagcaa ggatagggca tctgcccttt tgattgtttg attaacgata
     5101 caaggagttt caaaatgaga gtt
//
LOCUS       DAWKOD010000144         5048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000144 DAWKOD010000000
VERSION     DAWKOD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.144"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..1720
                     /gene="pilB"
                     /locus_tag="WJK73_001729"
     CDS             44..1720
                     /gene="pilB"
                     /locus_tag="WJK73_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ7558173.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGLLKVMQGITSLEEVTANTND"
     gene            complement(1805..2014)
                     /locus_tag="WJK73_001730"
     CDS             complement(1805..2014)
                     /locus_tag="WJK73_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ7558174.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(2007..2639)
                     /locus_tag="WJK73_001731"
     CDS             complement(2007..2639)
                     /locus_tag="WJK73_001731"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ7558175.1"
                     /translation="MAVWVGLTGGIGSGKSAAAQCFADLGVPRIDADVAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFTSLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLCEKTMRLHAFYSGIFASKPTQGKHND"
     gene            complement(2641..3501)
                     /locus_tag="WJK73_001732"
     CDS             complement(2641..3501)
                     /locus_tag="WJK73_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ7558176.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3575..4807)
                     /gene="pilG"
                     /locus_tag="WJK73_001733"
     CDS             complement(3575..4807)
                     /gene="pilG"
                     /locus_tag="WJK73_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ7558177.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
BASE COUNT         1178 a         1259 c         1412 g         1199 t
ORIGIN      
        1 gctgtacagg tataacaatg aataaatttg gggataaggt cgtatgagcg taggtttgct
       61 gaggattctg gttcaaaacc aggtggttac tgttgagcag gccgagcatt actacaatga
      121 gtcgcaggcg ggtaaggaag tgttgccgat gctgttttca gacggtgtca tttcgcccaa
      181 gtcgcttgcg gcattgattg cgagggtgtt cagttattcg attcttgatt tgcgtcatta
      241 tccgcgccac agggtgctga tgggggtgtt gacggaggag cagatggtgg agttccactg
      301 tgtgccggtt ttccgtcggg gcgacaaagt attttttgcg gtttccgatc cgacacagat
      361 gccgcaaatt cagaaaaccg tttctgccgc agggattgag gttgaattgg tcattgtcga
      421 ggatgaccag ttggcgggtt tgctcgattg ggtgggttcg cgttcgacat cgctgcttca
      481 ggagcttggg gaggggcagg aggaagagga aagccacacc ctgtatatcg acaacgagga
      541 ggcagaagac ggccctgttc cgaggtttat ccataagact ttgtcggatg ccttgcgcag
      601 cggggcatcg gacatccatt tcgagtttta cgaacacaat gcccgtatcc gtttccgtgt
      661 ggacgggcag ctccgcgagg tggttcagcc gcccattgcg gtaagggggc agcttgcttc
      721 ccggattaag gtaatgtcgc gtttggacat ttccgaaaaa cggataccgc aggacggcag
      781 gatgcagctg acctttcaaa agggcggcaa gcctgtcgat ttccgtgtca gcacattgcc
      841 gacgctgttt ggcgaaaagg tcgtgatgcg gattttgaat tccgatgccg cgtctttgaa
      901 catcgaccag ctcggttttg agccgtttca gaaaaaattg ttgttggaag cgattcaccg
      961 tccctacggg atggtgctgg taaccggtcc gacgggttcg ggtaagacgg tgtcgctcta
     1021 tacctgtttg aatattttga atacggagtc ggtaaatatt gcaacggcgg aagaccctgc
     1081 cgagattaac ctgccgggca tcaatcaggt taacgtcaat gataagcagg gcctgacttt
     1141 tgccgctgct ttgaagtctt tcctgcgtca ggacccggac atcattatgg tcggtgagat
     1201 tcgtgatttg gaaactgccg atattgcgat taaggcggca caaacagggc atatggtgtt
     1261 ttccaccctg cacaccaata atgcgccggc gacgttgtcg cgtatgctga atatgggtgt
     1321 cgcgccgttt aatattgcca gttcggtcag cctgattatg gcgcagcgtc ttttacgcag
     1381 gctgtgttcg agctgcaaac aggaagtgga acgcccgtct gcctctgctt tgaaggaagt
     1441 cggcttcacc gatgaggacc ttgcaaaaga ttggaaactt taccgcgctg tcggttgcga
     1501 ccgttgccgg gggcagggtt ataaagggcg tgcgggcgtg tatgaggtta tgcccatcag
     1561 cgaagaaatg cagcgtgtga ttatgaacaa cggtacggaa gtggatattt tggacgttgc
     1621 ctataaggag ggtatggtgg atttgcgccg ggccggtttg ttgaaagtta tgcagggcat
     1681 tacttcattg gaagaggtaa cggcaaatac caacgattag gtttgagaat gaaaatgccg
     1741 tctgaagcgt gtttgtttca gacggcattt gactttcagg gtgcttgcag ggaaggcggg
     1801 gcggtcagcg gtatgccatg tcgggttcgg atatttccgg caaactttcc gtttggccgg
     1861 aaaccgtata tttcccgtct gcccatccgc ccaagtcgat cagtttgcag cgttgcgaac
     1921 agaaggggcg gaatgcgttt tcgggttccc atactactgc cgtttgacag gtcggacatt
     1981 tgacttgaag gcgtgtttgc cgcgattcag tcattgtgtt ttccttgtgt tggttttgag
     2041 gcgaaaatcc ctgaataaaa cgcgtgcagg cgcattgttt tctcacacag gcttttgagg
     2101 ctgccgtcat tgagcagcac atcgtctgca agcagcaggc gttcggattc ggatgcctga
     2161 tggctgatga tgtccgccac ctcgccgcgc gtcagcccgc tgcgggccat caccctgccg
     2221 atacgttttt ccaaaggtgc gcttatggtc aggacacgcc gtatcaggct ggtaaattga
     2281 cgcttttccg tcagcagcgg gatttcgaca atgccgtagg ccgcatcggt aaaggtttct
     2341 tgctgctttt tgatttctga gaaaatcagc ggcaacatca cggattcgag caaggctttg
     2401 cgtgatgggg aggcaaagat ttctttacgc aatatgtcgc gccgcaacaa accctgtgtg
     2461 tcgaaaacgg tgtcgccgaa cagccgcctg atttccggca gggcgatgcc gtctgaagcc
     2521 gtcagcgagt gcgccgccac gtccgcatcg atgcgcggca cgcccaaatc ggcaaaacat
     2581 tgcgcggctg ccgatttgcc gctgccgatt ccgccggtca gcccgaccca taccgccatc
     2641 ttacagcacc ggatgggtca gccaccagtt gaccgcccgc catacggaat cgtttgccgt
     2701 aaaaattatc cagcccgaaa ctgtcagtgc ggggccgaag gcaaaatgct gccccttggc
     2761 gacgcgcata acgattgccg cgaccaaacc gatcagcgag gaaacaaaaa tcagcacggg
     2821 caatgcggat atgccgagcc acgcgcccaa tgcggcaatc agtttgaaat ctccgttgcc
     2881 cataccgatt tcgcctttaa tcagtttata taccgcgcat aagagccata atgaaccata
     2941 gccggcgacc gcacctaaaa cggcagactg caaaggcacg aagccgccgt ccaaattaaa
     3001 tatcagcccc agccaaatca agggcagtgt catcgagtcg ggcaggtatt gggtgtccgc
     3061 atcgataaag gtcagggaaa tcagaaacgc ggtcagtatc aaaccgccca atgtaatcca
     3121 agaccagccg tattgccagg caaccagccc gaacaatacg ccggtcagca gttcgattaa
     3181 gggatagcgt atgctgattt tggtttggca ggaggcgcat ttgccgcgca ggagcaggta
     3241 gctgacaatc gggatgttct gccacgcgcg tatcggcacg cggcatttgg gacagcagga
     3301 atccggtttc atcaggttga aggtacggct ttcctcttcg gtcagcggca ggtttaaata
     3361 ttctttggca aataccgtcc agccgcgttc catcatgacc ggcacgcggt aaatgacgac
     3421 atttaagaaa ctgccgacca gcagcccgaa caccgctgcc aaaggcacgg caaacggcga
     3481 caatacagac aaatcagaca tattttgttc tcaatgtatt caaaacaaaa acaaaccggc
     3541 gcagagcgaa tccgcgccgg atctgtgcgg caaatcaggc gaccacgttg cccaaattaa
     3601 acagcggcag atacatggcg accagaagcg tgccgatgac caagcctaaa atcacgataa
     3661 tgatcggctc catcatagcg gacagcctgc cgaccgcatt gtccacctcg tcttcgtaaa
     3721 attcggcggc tttgttgagc atatcgtcca aagaacccga ttcctcgccg atggaagaca
     3781 tctgcaacat catattgggg aacagttccg tcgcacgcat ccccgaagtc atagacaaac
     3841 cttggatgac gcgcgtacgg atttcccggg tggcttcttc atagattaaa ttgcccgccg
     3901 cgccggcagt ggagtccaat acatcgacca aaggcacgcc tgccgcaatc agcgtcgccg
     3961 tcgtcctgcc ccagcgggca atcgtccctt tgcggacaat gtctccgaaa atcggcatac
     4021 gcagcagtat ggcatccata cgccgttgga ttttaatcga acgcgccttc aatttaagga
     4081 agccgtatat ggcaaagccc agtgcgatca gcaccatcca gccgtatgag acgaaaaagt
     4141 cggacatatc catcactgtt tgggtcagtg cgggaagctc cgcgcccata ttggcgtaaa
     4201 cttctttaaa ggcgggcagt acgaaaatca tcatcacgaa taccaaaccg atggcgacgg
     4261 cgatgacgga taccggatag gtcagtgcgg tttttacctt tttgcggatg gcctgggttt
     4321 tttctttgta aattgccaat ttgtccagca ggctttccaa tacgccgccc gtttcgcccg
     4381 ccgcaaccag attgcagtag aagcggtcga aatattttgg gtggtttgag aatgcgcggc
     4441 tcaacgagct gccctgttcc acttcgcctc ggatttccat cagcatttcc gtcatagacg
     4501 ggttgccgtg tccgcgcgcc acgatttcaa atgcctgcat cagcggcagg cccgctttaa
     4561 tcatcgtgga cagctggcgg gtgaaaacgg tgatgtcttc ttgtgtgatt ttgcgcttgg
     4621 agcttgtttt cacacgggta atctgcaacg ggcggatgcc gcgttttgcc agttttttgc
     4681 gcgcctcttc ttcggtaaac gcggatactt cgccgttgac cagtttgtcg gaggcggaat
     4741 gcctgccttc aaagataaag cgtttttctt tctttgcgaa caaagaaaat cctccgtttt
     4801 tagccatatt ctagccccgt aaagtaattg gaataaaatg taagaaacat cgttaaaaaa
     4861 cagtaccggc gtgttcccgg taagatgaaa accgccgaca tcccgcctgc gggcggcaaa
     4921 cgggacagaa tcggatgcga ttatacctta tttaggcggc tgtccggcat ttatgcgtac
     4981 acaataaatc ttgcaggata ttgttgcggg tcaaatgccg gccggagggt atagtgaatt
     5041 aacaaaaa
//
LOCUS       DAWKOD010000145         4829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000145 DAWKOD010000000
VERSION     DAWKOD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.145"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            245..982
                     /locus_tag="WJK73_001734"
     CDS             245..982
                     /locus_tag="WJK73_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7558178.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIEARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            984..1670
                     /locus_tag="WJK73_001735"
     CDS             984..1670
                     /locus_tag="WJK73_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7558179.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPLAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1829..2692
                     /locus_tag="WJK73_001736"
     CDS             1829..2692
                     /locus_tag="WJK73_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558180.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2755..3324)
                     /locus_tag="WJK73_001737"
     CDS             complement(2755..3324)
                     /locus_tag="WJK73_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ7558181.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSTEWQGMAD"
     gene            3482..4342
                     /locus_tag="WJK73_001738"
     CDS             3482..4342
                     /locus_tag="WJK73_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ7558182.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4477..4815
                     /locus_tag="WJK73_001739"
     CDS             4477..4815
                     /locus_tag="WJK73_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558183.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
BASE COUNT         1194 a         1457 c         1189 g          989 t
ORIGIN      
        1 tttcaattat ttgccgtttt ggtgcgaacc gctgcctttg cccgtttcag acggcattgt
       61 ccgaaatggt tgcctgcttc ctgccttatt gacaaaaaaa tgctttcccg ataatatcct
      121 acgaaaatta acctgccgat ttgacacagc ttgcgggcat aacagctaaa gcgttccgac
      181 aattccagct ttatcttccg cgcccgttgt gtccgacatc gggctttgtt gtatgggaaa
      241 gacaatgatt attttggaca aggtttccaa gcattaccaa acgcgcgaca agacccgttt
      301 tgccgctgtc gagccgacca gcctcgaaat ccgcgacggt gaaatcttcg ggctgatggg
      361 ttattcgggt gcaggcaaat ccaccctgtt gcgcctgatt aacctgttgg aacgtcccga
      421 cagcggcaag gtcaacgtct gcggacaaga gctgaccgcg ctcgatgccg ccgcattgcg
      481 tcaggctcgg cagaatatcg gcatggtgtt tcagcagttc aacctgttga acaaccgcac
      541 cgtcgccggc aacgtcgcct tcccattgga aatcgccgga tggccgtctg aaaaaatcga
      601 agcgcgcgtt gccgaatgtc tcgaaatcgt cggactgacc gaacgcgccg gtcactatcc
      661 cgcccagctt tccggcggac aaaaacagcg cgtcggcatc gcccgcgcgc tcgcatccaa
      721 gccgcaagtc atcctcgccg acgaacccac ttccgccctc gaccccgcca ccacgcgcag
      781 cgtcttggaa tgtttggaag acatcaacaa acgcttcaac gtaaccatcg tcatcgtaac
      841 ccacgaaatg agcgtcatca gacgactgtg cgaccgcgcc gccctgttgg ataagggaaa
      901 agtcgtcgaa atcgtcgaag tacgcggcaa ccaaatccac gcccgatccg acatcgggcg
      961 cgaactcatc agagaggact gaaatggcag acctgacctt ccaacaagcc gtttccaaca
     1021 tcatcggtat gaaaggcgaa atcgtccgcg cattgggcga aaccttcgtg atggtcggct
     1081 tgtccaccac attcgccgtc gttttcggca cgttgctggg cgtgttgctc ttcgtaactt
     1141 ccagccgcca actgcattac aacaagccgg tgaacttcct gctcgacaac ctcgtcaacc
     1201 tcatgcgcgc cttccccttc gtcattttga tgattgcgat gatacccgcc acacgcgcca
     1261 tcgtcggcag caccatcggc ccgattgccg cctcgctggt gttgagcgtg tcgggattgt
     1321 tttattttgc ccgactggtg gaacaaaacc tgcgcgaagt ccccaaaggc gtgattgaag
     1381 ccgccgccgc gatgggtgcg ccgccgcttg ccatcgtccg caaagtcctc ttaaacgaag
     1441 cgcgcgcggg tatggtttcc agcattaccg tgcttgccat cgggcttttg tcatacagcg
     1501 cggcggcagg gatgataggc ggcggcggct tgggcgacct cgccatccgc tacggctact
     1561 accgctacca aaccgaagtc atcgtcttca tcgtcgccct cctcgtgctg ctggtcatcc
     1621 tgattcaaag caccggcaac gcgttggcgc ggaaactcga caaacgttga acccgaatgc
     1681 cgtctgaacg ccaaaacccc caccgctatc cgaaaaatgc tataaaatcc ccctgttcgc
     1741 ggcaaatgcc gtctgaacgc cgaatccgga cggcaggact ccctgcccgt catttttgtt
     1801 tgaaactgcc acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc
     1861 cgcactcgcg ctcatcctcg ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc
     1921 cgcttctgcc gccgccgaca acggcgcggc gaaaaaagaa atcgtcttcg gcacgaccgt
     1981 cggcgacttc ggcgatatgg tcaaagaaca aatccaagcc gagctggaga aaaaaggcta
     2041 caccgtcaaa ctggtcgagt ttaccgacta tgtgcgcccg aatctggcat tggctgaggg
     2101 cgagttggac atcaacgtct tccaacacaa accctatctt gacgacttca aaaaagaaca
     2161 caatctggac atcaccgaag tcttccaagt gccgaccgcg cctttgggac tgtacccggg
     2221 caagctgaaa tcgctggaag aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc
     2281 gtccaacttc gcccgcgtct tggtgatgct cgacgaactg ggttggatca aactcaaaga
     2341 cggcatcaat ccgctgaccg catccaaagc ggacattgcc gaaaacctga aaaacatcaa
     2401 aatcgtcgag cttgaagccg cgcaactgcc gcgtagccgc gccgacgtgg attttgccgt
     2461 cgtcaacggc aactacgcca taagcagcgg catgaagctg accgaagccc tgttccaaga
     2521 accgagcttt gcctatgtca actggtctgc cgtcaaaacc gccgacaaag acagccaatg
     2581 gcttaaagac gtaaccgagg cctataactc cgacgcgttc aaagcctacg cgcacaaacg
     2641 cttcgagggc tacaaatccc ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg
     2701 tataaaatga tgccgtctga accgcattcc gtgttcagac ggcatttttg tcctttaatc
     2761 cgccattccc tgccattccg tcgaatccgg tgtatcgatt ccgaacagcg acaaagcgtg
     2821 tgcaacactg tgcgccacta tgtcgtccgc cgtctgcggt ttgcggtaca tcgcaggaac
     2881 agggggaaac accacgccgc ccatttccgt tacccgcttc atattgtcca aatgggcaag
     2941 gttcagcggc gtttcgcgca ccatcagcac cagccgccgc ctttccttca aaaccacatc
     3001 cgccgcacgc gtcaagaggt tgtcgccgaa gccgtgcgcg accgaggcaa gtgtccgcat
     3061 cgaacagggg gcgaccagca tcccgtccgt tttaaacgta ccgctggcaa tgcacgcccc
     3121 gatattgccg atcggatgca cgaagtccgc caaggcatat acctcatctc tcgcataagc
     3181 cgtttccgaa gcgcgcgcca tctccgcacc tttcgatacc acaaggtgcg tttcgatatc
     3241 ttgcgcgcgt aaaagttcca aagccttcac gccgtattgg aaaccgctcg ccccactgat
     3301 gccgattatc aaacgccgta ccatcatccg cctttcccat aaaaccgcct gcaacggcaa
     3361 accggctatt atagtgaaaa aacagaaatc cgataaatac ggatacaaat tgtcggcaac
     3421 acccaatatc cgataaaata tccgatttaa catcctatct gaataggcac gggagggcgg
     3481 tatggcaaaa gtaaaaggcg gattggggcg cggtttggat tcgctgctcg ccaacggcgc
     3541 agacaacagc agcggcgacc gattgaccac ggttgcggtt aaagatatcc ggcccggccg
     3601 ctatcaggcg cgtgttcaaa tcgatgacga agccttgcag gagctggcag attcgattaa
     3661 ggcgcaaggc gtgatacagc ccgtcatcgt gcgcgaacac ggactgtccc gatacgaact
     3721 gattgcgggg gagcggcgtt ggcgtgccgc acagattgcc ggcctgaccg aaatccccgc
     3781 cgttatcaaa accatcagcg acgaaaccgc gttggcaatg ggtttgatcg aaaacctcca
     3841 gcgcgaaaac ctcaacccca tcgaagaagc acaaggcttg aaacgccttg ccgacgagtt
     3901 cgggctgacc cacgaaacca tcgcccaagc cgtcggtaaa agccgaagcg cgatttccaa
     3961 cagcctgcgc cttttaagcc tgcccgaacc cgtgcaggaa atgctttacc aacgccgcct
     4021 cgaaatgggg cacgcccgtg cactgctgac cctgcccgtc gtcgaacagc tcgaattggc
     4081 gcaaaaggct gtcaaaaacg gctggtcggt gcgcgaagtc gaacgccgca gccaagccgc
     4141 ccttcaaaac aaacgtcccg agcccaaaaa aactgccgcc gccgacatcg gccgcctgaa
     4201 tgatttgctg actgaaaaac tgggtgtcaa cgccgaaatc aaaaccgcca accataaaaa
     4261 aggcagaatc atcctgcatt tcgacacgcc cgaaaccttc gaccatatct tgaagcagtt
     4321 gggcatagat taccgacctt aattttgcgg gatataccgt ctgaaatata gagaatagct
     4381 ttccagattt taagtgggaa atataattct attgacattt ttctgcttca cgtaagaatc
     4441 gttttcctgt tttcattttt aattttcgag gaaattatga acacacgcat catcgtttcg
     4501 gctgcgttcg ttgcgttggc attagcaggt tgcggctcaa tcaataatgt aaccgtttcc
     4561 gaccagaaac ttcaggaacg tgccgcgttt gccttgggcg tcagcccaaa tgccgtaaaa
     4621 atcagcaacc gcagcaatga aggcatacgc atcaacttta ccgcaactgt gggtaagcgc
     4681 gtgagccaat gctatgttac cagtgtaatc agcacaatcg gcgttaccac ttccgatgca
     4741 atttgtttgg gaggcggaac gcacaaaggc aaaagtcaat gcaatgcttt gcttaaagcg
     4801 gcaggccgtt gctaatcctt tattcggaa
//
LOCUS       DAWKOD010000146         4823 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000146 DAWKOD010000000
VERSION     DAWKOD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.146"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..554)
                     /locus_tag="WJK73_001740"
     CDS             complement(72..554)
                     /locus_tag="WJK73_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7558184.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(707..868)
                     /locus_tag="WJK73_001741"
     CDS             complement(707..868)
                     /locus_tag="WJK73_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558185.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(942..1403)
                     /gene="recX"
                     /locus_tag="WJK73_001742"
     CDS             complement(942..1403)
                     /gene="recX"
                     /locus_tag="WJK73_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ7558186.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1476..2186)
                     /locus_tag="WJK73_001743"
     CDS             complement(1476..2186)
                     /locus_tag="WJK73_001743"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ7558187.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2593..3861
                     /locus_tag="WJK73_001744"
     CDS             2593..3861
                     /locus_tag="WJK73_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ7558188.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIM
                     SWMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGA
                     TYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITN
                     EAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVI
                     LRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNL
                     AQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3919..4725)
                     /locus_tag="WJK73_001745"
     CDS             complement(3919..4725)
                     /locus_tag="WJK73_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ7558189.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1034 a         1090 c         1428 g         1271 t
ORIGIN      
        1 cttgatttca tccgtttgca atggaaacgg atggcgcgat gtgcctgtct ttcagacggc
       61 atagtccgcc gtcagcagcc gcaggacatg tcttcagagg cttgcaggct gatgtctctg
      121 cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg tcaggatttc cagcggaggg
      181 acagggacgg gtttgccgtc tttgaccgca accatcgtga agtagcagct gttggtatgg
      241 cggatttcgc cggtgcggat gttttgtgct tcgacacgga tgccgatttc catcgaggtg
      301 cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat cgccgatgtg gatgggttct
      361 ttaaacagga ctttgtcaac cgacagggta acgcaataat tgccgctgta acggctggcg
      421 caggaatagg cgacttggtc gagcaggagc aggagttcgc cgccgtgtac gttgccgctg
      481 aaattggcgg tgtccggcat catcagttcg gacataatga gttcgtgcga aggcagttgg
      541 cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt tttgaaatgg gtgcggggaa
      601 attttaggga atggggaagt ggaattcaag ctaaaaaaag ctatggcggg atagtctgca
      661 gaaatgggcc gccgatgccg tctgaagcct tcagacggca ttttgtttag gaaagccaaa
      721 aacggaagac caggaaaaac acgagtgcca atacggcgac ccaaacgacg agcatgaatc
      781 cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg tttgtcttcg atgggggagg
      841 tgtggtgttt ttcgggttgt ccggacattt tgttctcaat cggaaaagtt ggaaacgggc
      901 gtaaggttat gttgccgtgc aaaaagattc aagaaggaag gtcagcagcc gtcttcccaa
      961 ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat cggcatcgaa tccgcgatag
     1021 gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg cggcggggtg tttgaatttt
     1081 ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc ggtcgggaag caggttgcgg
     1141 ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt tcagcctcaa tgaaccgtgt
     1201 ttgcggcttt tgctgcggat ataggcttcg gcatagcgca aatccgactg ccagttgcgt
     1261 tcggcaaatt cgtttaacac gttttccaac tcctcttcgc tttcggcgtg cggtgcaagt
     1321 ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga ggatgtccat cgcgcgggcg
     1381 cgtagggatt tttgcggttt catggcggcg gtttcggcac ggaaagattc agacggcatg
     1441 ggaaacggat tccgatgccg tctgaacaga ttgttttatt cttcgtcttt atttttctgc
     1501 ggttgcaggt tttcgtaaat ttcgggcagc gagccgataa tccagtcggg gcgggtcgca
     1561 tcgtcttgcg agagcagcgt catatcgccg taaccgaagg taacgccgac gctcaggcag
     1621 ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc cgaccataac catgtttgca
     1681 acatcgatac ccaaaacttc ggcggcgtgc cgcagcggca gggggctggg ttttttctcg
     1741 ggcaggctgt cgccgccgag tatcaggctg aagtagtcgg caagtccgag ctgtttcaac
     1801 agttcgacgg caaggatttc gtttttattg gtaatgactg ccagcgggat gcccaaagat
     1861 ttgagcaatg ccagcccggt tgcggtttcg ggataggggc gggtgaagac gctcaaatgg
     1921 tcgcggtagt atttcatata ggatacgaaa cctttttccc acagttcgga atcggcttcg
     1981 cggtcgcggt cgttggagag gacgcggtga accagtttgc cgatgccgtc gcccacatag
     2041 ctttcgacca ctttggcggg cagcggtttc ataccgagtt gttccaacat cgcttctgcg
     2101 gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt ccaaatcgaa ggcgacggct
     2161 tggacgtgtt cgatggctgc attcataatc tgatttcctg tcgggttgaa agggtttatt
     2221 ttaacatcgt ttgcacaggg cttctaatgt atagattgtt aacaatcgca acaggttttg
     2281 caggcggtgt tttcggattt cggggcggaa tcgggcaagg gtttgaaatc ccagcgatgt
     2341 tccaattaaa tttttaaaaa ataaaaggat gcctatatct ttatttaatt tgacatttat
     2401 ttttcggacg cgcgcgctgc tttttcaggc ggagtccggg ctgattccgt gcggcttgaa
     2461 aaataaaatc atatatagat taatcaaatc gggcttatgc cgactacacg ctattacatt
     2521 ttgtcgggaa attggttata gtaatgagcg aaatgcacgc ccctgaaaat ctttttgtga
     2581 aaaggaagca aaatgtcgtc tgaagccctt gcaaaagaaa ccctcaatcc gtttgaaatc
     2641 gcgcgcaaac aggtcaaaac cgcctgcgac cggctgaaaa ccgatccggc cgtttatgaa
     2701 atcctgaaga gtccgacccg cgtgttggaa gtcaatttcc ccgtcaaact cgacgacggc
     2761 acggtcaaaa ccttcacggg ctaccgttcg caacacaaca acgccgtcgg cccctataaa
     2821 ggcggcgtgc gcttccatcc cggagtgaat ctggacgaag tcaaagccct gtcgatttgg
     2881 atgaccatca aatgctgcgt tgcgggtatt ccttacggcg gcggcaaagg cggtattacc
     2941 ttggatccgc gcgattattc cgaggcggaa ctggaacgca tcgctcgcgc ttattccgaa
     3001 gccatttctc cgctgattgg cgagaaaatc gatattcccg cccccgatgt gaataccaac
     3061 ggcaaaatca tgtcttggat ggtggatgct tatgaaaacg tggtgaaaca ttccgcgccg
     3121 ggcgtattta ccggaaaacc ggttgagttc ggcggctctt tggcgcgtac cgaggctacc
     3181 ggttacggcg tgaacctcgc cgccgtccaa gctttggaaa aactgggcaa ggacgtgaag
     3241 ggtgccactt acgccattca aggtttcggt aatgtgggtt accacacagg ttattacgcg
     3301 catcaatccg gcgcgaaagt tgttgcggtt tctactgtcg atgttgccat ttacaacgaa
     3361 aacggcttgg atatggaagc tttgtttaaa gagttccaag aaaaaggctt catcaccaac
     3421 gaagccggtt acggcaaaga aattaccaac gccgaacttt tggctttgga tgtggacgta
     3481 ctcgcccctt gcgcgctgga aaaccaactg acttccgaaa acgccggtaa agtccgcgcg
     3541 aaaatcgtgg tcgaaggcgc gaacggcccg actacgcccg aagccgacgt tatcctgcgt
     3601 caaaacggcg tattggtcgt gcctgatatt ttggcgaact gcggcggcgt ggtggtatct
     3661 tattttgaat gggtacaaaa cctgcaaggc tattattggg agtttgacga agttcaagag
     3721 aaagaaaccg tcgtcctgcg acgcgcgttc cgcgatatat ggaacttggc tcaagaatac
     3781 gatgtcgatt tgcgaaccgc gtcttacatg atgagtatcc gccgtgttga aaaagcgatg
     3841 aagctgcgcg gttggtattg atgtttgaac aaaaaatgcc gtctgaagcc ttcagacggc
     3901 atttttgtgc gggcgggatt aatggtgcga atggccgatt tggaaggtta aagtcgagta
     3961 gttcgcctgt ttttggcaca cgctttgatc ggggaagtcg gctttgtgtt cgacattggc
     4021 tttccagaag ccttggcgca aggggatgat gtccacttcg cctttgtcgt ctgtgctgtc
     4081 ggagaaagcc tgtgcttcgg ttttgtgcgt tttgctgcgg tcgctggtgt cgaagccgtc
     4141 aaaggtggcg gtaacggtgg cattgggcag cggttcgcca cggaacagaa cgcggacttt
     4201 gaagcgttcg cctacgtgaa tgttggcggg attgtccagc gggacgattt ccaagttttg
     4261 tccgaccggt ttggtgatga tggcggtgtc cgcgctttcg tgtccgacgt tgacgatgtt
     4321 tttgccgaac attcgggttt gttcgcaata gcttgcgtca ggcatttctt tgatgcccgc
     4381 ctgtttccag cctgctttgt tttttgacca gaaagtaggc tgatattcgg cgatgacgag
     4441 gtaactgccg tccttaacgg gacggttgct tcggtactgg tagttgtatg tgccgcgttg
     4501 aatcatgttt tccttgcctt tttcggtaac cagctgcatc ggtttgctga agatgtgcag
     4561 gcggtctttg gcgatgggtt cgagttcggg aaattcgccg tagcccaagt cggctttaag
     4621 gtattcgccg ccgtgcgtgt gggcggtttc gacccagacg cggtgggcgt gggcggatgt
     4681 ggcaaatagg gcggaaacgg cgagcagtgt caatgttttc ttcatggtgc agcctttgtc
     4741 tcgtgtgggt taaagatttt gttatgttat aacaaaatag gtatcgtgtg aagatggatt
     4801 tgctgtctga aaactgtctt ttt
//
LOCUS       DAWKOD010000147         4769 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000147 DAWKOD010000000
VERSION     DAWKOD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.147"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1019..1456)
                     /gene="rnhA"
                     /locus_tag="WJK73_001746"
     CDS             complement(1019..1456)
                     /gene="rnhA"
                     /locus_tag="WJK73_001746"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ7558190.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1570..2439
                     /gene="tehB"
                     /locus_tag="WJK73_001747"
     CDS             1570..2439
                     /gene="tehB"
                     /locus_tag="WJK73_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ7558191.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCVAEDYFH
                     KKYGMSATHSAVAAAQDIVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            complement(2489..3295)
                     /gene="thiD"
                     /locus_tag="WJK73_001748"
     CDS             complement(2489..3295)
                     /gene="thiD"
                     /locus_tag="WJK73_001748"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ7558192.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            3356..3553
                     /locus_tag="WJK73_001749"
     CDS             3356..3553
                     /locus_tag="WJK73_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558193.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(4309..4557)
                     /locus_tag="WJK73_001750"
     CDS             complement(4309..4557)
                     /locus_tag="WJK73_001750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558194.1"
                     /translation="MNRHSHTLRHSRENGNLERRVEETVLSDKFPCRQGWIPACAGMT
                     VRGCRLKRWAEAHPTIVSEASPTIVSASIPFGGIFSIL"
     gene            complement(4597..4672)
                     /locus_tag="WJK73_001751"
     tRNA            complement(4597..4672)
                     /locus_tag="WJK73_001751"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4636..4638),aa:Glu,seq:ttc)
BASE COUNT         1062 a         1173 c         1335 g         1199 t
ORIGIN      
        1 ggtttttgtt aatccactat agtttaattt gtctgtccgc attgccgcta attgtaccac
       61 ttcatcgtat ttccttttcg gttgaaaccc cgccctttag ggcggcagga tcagacttta
      121 tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt gctttatgtg
      181 tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca aactgtttta
      241 gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc attcccacac
      301 aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg ctacggttac
      361 taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca gttgtttctg
      421 ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac ggtagagcgg
      481 tttcagttgc tcccgataaa tgccgcaatc tcaaatcccg tcattccctc aaaaacagaa
      541 aaacaaaatc agaaacctaa aatcccgtca ttcccgcagc tcttcgtcat tcccgtgaaa
      601 gcgggaatct agacctttta aattttcaca cggcttttga atattgccgt tacccgaagg
      661 tctggattcc cgcctgcacg ggaatgacgg cagagcggtt tcagttgttt ctaaataaat
      721 ttttgcagcg ttggattttt agattcccgc tttcgcggga atgacggggc ggtcggatgc
      781 cgcctgaagc ccgacaaagc ccccaatgcc gtctgaaacc tcgtcattcc tctgctgtcc
      841 gcaacttttc gtcattcccg caaaagcggg aatctagagc ctctaaattt tcagacggct
      901 tttgaatatt gccgttgccc gacggtttgg attcccgcct gcgcgggaat gacggtggag
      961 gacgatgccg tctaaagccc attagcggtt tcagacggca tttttgccgg agcggcagtc
     1021 aggaaaactg cgctgcgcca cggtttgcca aatcgtcggc gcgttcgttt tccgcgtgtc
     1081 ccgcgtgtcc tttaacccaa gtccagctga cttggtgctg cccaaccaga acgtcgagtt
     1141 ctttccacaa gtcgtcgttt ttgacgggct gtttggcggc ggttttccag ccgttgcgct
     1201 tccaaccgtg tatccagttt tccatgccgt ttttgacgta ttgcgagtcg gtgcagatga
     1261 tgacggtgca gcggcgtttg agcgatttca gtccttcgat gacggcggtc agctccatgc
     1321 ggttgttggt ggtttgcgct tcgccgccga aaagttcttt ttcgcggctg ccgtagcgca
     1381 tcaacacgcc ccagccgccc gcgccgggat tgcctttgca cgcgccgtcg gtgtaaaggt
     1441 aaacggtttt gtccattatt gtgcctttgt gtggtgtccg gcgtgattat aagcggtttg
     1501 tgcgccgtgc cgtctgaaag attgcggcgg attaaaatag gcatataatg tgcaacttta
     1561 tttttgaata tgaaggagtg gatagtgggt caaagcggcg agttgttttg tttcggacag
     1621 atgcccgtat ggaaggcgga aaacctgccg gaagttttgt tgtcgggcta ttcgtccgaa
     1681 ggaggggaat gggtctgcct gaatgtgttg cagggcgatg tcgaagtccg cacgcaggac
     1741 ggggcggcgg aggtttggtc ggcagaaagc ggcgattgcg tgtttgcgcc gcagcaggtg
     1801 ttttcggtca aaccgaaaac ggacgatgcc gaaatccgtt tgtcgatgta ttgtgtggcc
     1861 gaagattatt tccacaaaaa atacggcatg agtgccacgc attctgcagt ggcggcggct
     1921 caggatatcg tacctgcggg cagggcgctc gatatgggct gcgggcaggg gcgcaacgcg
     1981 ctgtttctcg gtttgaaggg gtttgaagtt actgcagtcg atcacaatcc cgccgccttg
     2041 gcaaacgtgg cggagctggc agaggcggag ggtttgaacg tccgcacgct ggaatatgat
     2101 ttgaacgccg ccgccttgca gggcgagttt gattatattg tggcaacagt ggtgctgatg
     2161 ttcctgatgc cgcagcgcgt acccgacgtg attgccgata tgcaggcgca tacggcggcg
     2221 ggcgggtaca acttgattgt atcggcaatg gatacggcgg atttcccctg cccgatgccg
     2281 ttccccttca aatttaaaga gggagaactg aaggattatt atcgggattg ggagctggtc
     2341 gaatataaag aagaattggg cgcgatgcac gccaaagacg aaaacggcaa tccgatacgg
     2401 tttaaatttg taaccatgct ggcgaaaagg cctgaataat cttcaaaaat gccgtctgaa
     2461 cattttgttt cagacggcat ttttacggtt agtcccgata ggcccaatga ttgaccgggc
     2521 cgtgtcccgc gccgatttcc aaagggcttg agattgccgc cgtgatgtag gacttggcag
     2581 tccgtacggc tttgcaaacg tctaagcctt ttgccaactc ggcggtaatg caggcagaaa
     2641 acgtgcagcc cgtgccgtgc gtgtgggcgg tcggaaagcg cgggctgtcc aattccagcg
     2701 tttcgttttg cgtaaacagc caatccgtgc agcgtccgct gctgctgcca ttcaaatgtc
     2761 cgcctttgat gacgacattt ttgacaccgt aatcaagcag gatttttgcc gcacgttccg
     2821 catctttacg gttttcgata tgcacgccgg tcagggcttc cgcctcgggc aggttggggg
     2881 tcaatacatc cgtatcggga agcagcaggc gcgtcagtgc cgcaacggcg gaatcctgca
     2941 acagcggcgc accgcctttg gcaatcatca cagggtcgag tacgcgcctg ccgaagtcgc
     3001 agtgtttcag cttgtcggca acgcattcga tgatttccgc cgtgccgagc attcctattt
     3061 tgtaggcgcg gatgtcaaag tcttccctga ttgcttggat ttgtgcggtg atggtttcgg
     3121 tcgggacgag atgaaccgcc gacacgccca aggtattttg cgcggtaacg gcggtgatga
     3181 cgcacgttcc gaacacgccg cgcatctgaa acgttttcaa atccgcctga atgcccgcac
     3241 cgccgcccga atccgaaccg gcgatggtaa gcgtttgcac aaaagagctt ttcatttgtt
     3301 cctccttaat attgtgtccg ataaaaaggg ctaatgcctg taaaaaagca cggcgatgcc
     3361 gtctgaaaac cgcagcgcgg gggtttgtgg tgcattatat gccgtattgc aggacaaggc
     3421 acagggcagg gcggcggggt gcatcgtagc aactgtattt ttgcccgacg gggtgaaaaa
     3481 tacagttgct acacccactg ataatgaaaa aagagagcaa tgccgtctga accgcgtgtt
     3541 gcggcgagca taaaccggca cgctttattc gtcatttcca caaaaacaga aacctaaaat
     3601 tccgtcattc ctacgaaagc gggaatccag tgtgttcggt ttcagttgtt tttgggtttc
     3661 gggtgatttc caaatcatca ttcccgcgca ggcgggaatc tagcccttgg ggcagcggca
     3721 atatttataa agccgtctga aaattcagaa gttctagatt cccgttttca cgggaatgac
     3781 gaagggttgt gggaatgacg aagtttcaga cggcatcgcc ccgccccgtc attcccgcat
     3841 aagagggaat ccaaaccatt gggcagagca gccttaaagg ttataaaaga ctcgtcattc
     3901 ccacgaaagt gggaatccag acctgtcggc acggaaactt atcggataaa acggtttctt
     3961 caaccctacg ctctagattc ccgcttttgc gggaatgacg aagtttcaga cggcatcaaa
     4021 tgctttgtca ggcttcagac agcatcgtcc accctctgcc gtccttcccg cgcaggcggg
     4081 aatccaatgt gttcggtttc ggttgttttt gggtttcggg taatttccaa atcgtcattc
     4141 ccacgaaagt gggaatccag accattgggc ggcggaaata ttcaaaagtc gtctgaaaat
     4201 tcagaggttc tagattcccg ttttcacggg aatgacgaag ggttgtggga atgacgaagt
     4261 tttagacggc attgaatact ttgatgcttt ggtgggctga agcccaccct acaatataga
     4321 gaaaatgccg ccaaacggta tggaagcgga aacaatggtg gggctggctt cagaaacaat
     4381 ggtagggtgg gcttcagccc accgctttag acggcatccg cgcaccgtca ttcccgcgca
     4441 ggcgggaatc cagccttgtc ggcacggaaa cttatcggat aaaacggttt cttcaaccct
     4501 acgctctaga ttcccgtttt cacgggaatg acgaagagtg tgagaatgac ggttcataaa
     4561 taaaaaaacc tgcttaaaac aagcaggttt ttgaattggc acgcccacgg ggattcgaac
     4621 cccggttgcc gccgtgaaag ggcagtgtcc taggcctcta gacgatgggc gcgtgggaat
     4681 gggcattata catacttcta gaaatttagc aatacgatgc cgtctgaaac ttcgtcattc
     4741 ccgcgaaagc gggaatctag aatctccaa
//
LOCUS       DAWKOD010000148         4707 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000148 DAWKOD010000000
VERSION     DAWKOD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.148"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..1503
                     /locus_tag="WJK73_001752"
     CDS             112..1503
                     /locus_tag="WJK73_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ7558195.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1565..2317)
                     /gene="rlmB"
                     /locus_tag="WJK73_001753"
     CDS             complement(1565..2317)
                     /gene="rlmB"
                     /locus_tag="WJK73_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ7558196.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(2384..2872)
                     /locus_tag="WJK73_001754"
     CDS             complement(2384..2872)
                     /locus_tag="WJK73_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ7558197.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2877..3596)
                     /locus_tag="WJK73_001755"
     CDS             complement(2877..3596)
                     /locus_tag="WJK73_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ7558198.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3600..4541)
                     /gene="miaA"
                     /locus_tag="WJK73_001756"
     CDS             complement(3600..4541)
                     /gene="miaA"
                     /locus_tag="WJK73_001756"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ7558199.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
BASE COUNT          872 a         1131 c         1514 g         1190 t
ORIGIN      
        1 tggtaaacgt gtgggccaga cggatgccgt ctgaaatgca aattgaagcc gtgcggcaga
       61 ttcgctacaa tccgcgcttg gatttttcaa cctttaaaat aaggaaatac aatgagcggt
      121 cagttgggca aaggtgcgga tgcgcctgat ttggtgtacg gtttggagga taggccgccg
      181 ttcggtaatg cgctcttgag cgcggttacc catcttttgg cgatttttgt gccgatgatt
      241 acgcccgcgc tgattgtggg cggcgcgctg gaattgccgg tggagatgac ggcgtatctc
      301 gtgtcgatgg cgatggttgc gtcgggtgtc ggcacttatt tgcaggtcaa ccgcttcggg
      361 ccggtcggtt cggggatgct gtccatccag tcggtgaatt tctcgttcgt tactgtcatg
      421 attgcgctcg gcgcggggat gaaagagggc ggtttgacta aggatgcgat gatttcgacg
      481 ctcttgggcg tatcgtttgt cggcgcgttt ttggtgtgtt tctcggcgtg gcttctgccg
      541 tatttgaaaa aggtgattac gccgacggtc agcggcgtgg tcgtgatgct cattggtttg
      601 agtttggtac acgtcggcat taccgatttc ggcggcggct tcggcgcgaa ggcggacggc
      661 acgttcggct cgatggaaaa cttggggctg gcatcgctgg tgttgctgat tgtgttggtg
      721 ttcaactgca tgaaaaaccc gctgttgcgc atgagcggca ttgcggtcgg gctgattgcc
      781 ggctatatcg tcgcgctgtt tttgggcaag gtggattttt ccgcgctgca aaacctgccg
      841 ctggttacgc tgcccgtacc gtttaaatat ggttttgctt ttgactggca cgcgtttatt
      901 gtggcgggcg cgattttctt gttgagcgtg tttgaggcgg tcggcgattt gaccgcaacg
      961 gcaatggtgt ccgatcagcc gattgaaggc gaggaataca ccaaacgcct gcgcggcggc
     1021 gtgttggctg acggcttggt gtcggtgatt gcgacggctt tgggttcgct gccgctgacg
     1081 acgtttgcgc aaaacaacgg cgtgattcag atgaccggcg tggcttcgcg tcatgtgggc
     1141 aaatatattg ccgtgatttt ggtgctgttg ggtctgttcc ccgttgtcgg tcgcgcgttt
     1201 acgacgattc cgagtccggt gttgggcggc gcgatggttt tgatgttcgg cttaattgcg
     1261 attgcgggcg tgcggatttt ggtcagtcac ggcatccgca ggcgcgaagc ggtgattgcg
     1321 gcaacgtcgg tcggtttggg cttgggtgtc gcgtttgagc cggaagtgtt taaaaacctg
     1381 cccgtcttgt tccaaaactc tatttccgcc ggcggcatta cggcagtctt gctgaatttg
     1441 gtcttgcccg aagataaaac cgaggcggcg gtcaagtttg ataccgacca cttggaacac
     1501 tgattttgaa aatgaatgcc gtctgaaaca gaatccctgt ttcagacggc attgtttttg
     1561 aggcttatac tttttcgttt tttaatacgc gttgtcggcg tgtttcactt aataccattc
     1621 cggcagacac ggagacgttc atgctttcga ctgtgccgaa catgggtatg gacaccagca
     1681 tgtcgcaatg ttcgcgcgtg aggcggcgca taccgtcgcc ttcgttgccc attacccacg
     1741 ccgcgctgtc gggcagattg caatggtaaa ggtcggactc gccgctcata tcggtgccga
     1801 taatccaaat gccgtattct ttcaattcgc gcagggtgcg ggcgaggttg gttacggtga
     1861 tataggggac ggtttccgcc gcaccgcagg cgactttgct gacggtggcg ttcagccccg
     1921 cgcttttgtc tttcggtgcg atgacggcgt gtacgcccat tgcgtcggcg gtacgcaggc
     1981 acgcgccgag gttgtgcgga tcggtgatgc cgtcgagtat cagcagcagc ggcggttcgc
     2041 tgaggttttc caatacgtct tcgaggtgga cgtggttttt ggaggcatcg ataaatccga
     2101 ccacgccctg atggcgcgcg cctttgctga tggcgttgag gcggtcggca tcgacaaaat
     2161 atacgcggat gttttcgttt gccgcctttt ccaacacttc gcgcgtgcgt gcgtctgatt
     2221 tgccttcttg gatgtagagt tcgacgatgg atttggggtt ttgccacaat cgggcgttga
     2281 cggcgtggaa gccgtagatg ggtctttggt ttgccatgat ggtgctttgt aaaaagggtt
     2341 cagacagcat tatagcaatt tgccggtatg ccgtctgaaa gggttaaaac aggtaggcga
     2401 tgtatttcac caacaggata aacaagatgg atacggcgca gccgattttg aacgccgtgc
     2461 cgacgacaag ccccaacagc gtacccaagc ccgctttacc tgcctgaagc atattgcgcc
     2521 gttcgatcag ttcgcctgcc gccgcgccga taaagggacc gagtattagt ccgggaaggg
     2581 agaaaaatat gccgatgatg ctgccggcca atgcgccgcg aacggcgagc ttgcccgctc
     2641 cggtatattt tgtcccccat atgcctgcca catagtccgc cagtatgccg gcaaggctga
     2701 tgagtccgac cgtccacaaa acgcccgcgc cgtagatttg gtagccgccg gcataggcaa
     2761 gcagccatgt tccggcaaac atcaatgcca atccgggcag ggcggggtaa acgatgcccg
     2821 ccgtgccgac ggctatcagg gcgagggcga ggatgacggt cagtacggtc ataggttcaa
     2881 ccttttcttt tgttttgaaa aaaacggctt aacacggcgc ggcattcttc ttgcaggatt
     2941 ccgccccgta tggcggtgtg cgtattgagg cgtttgtcgg caaacaggtt gacgatgctg
     3001 cctgccgcgc cggttttggg ttctgccgcc ccgtagatca cgcgcctgat tcgtgcctgt
     3061 atcagtgcgg acgcgcacat ggcgcagggt tcgagggtga tatatatgtc gcatccgtca
     3121 aggcggtagt tttgcatttc gctgcctgcc tgtgccaagg cgttgatttc ggcgtgccgg
     3181 ctgacattgc agtcggcaat gcaggtgttg tgtgccgatg cgatgatttt gccgtctgaa
     3241 acgatgactg ccccgacggg tatttcgccg tcagcggcgg attgttctgc ttggcgcagt
     3301 gcttcgcgca tgaagtgttc catttcttcc tgcggcggaa aggcggcgac gggcggatgg
     3361 tttttcaatt cggcaaaaag gcgcgttttg tcgtgttgcg tgatttcttg cgggagtgtg
     3421 ccgttcagca gcgactcaag ctgccacagc gtgcttttcg tgagggtcaa acccgatgct
     3481 ttgagcagca gaaaggcttt gaccgaaccg ttttgccgca gttcttcgag cgtacggata
     3541 ccgagcctgt gcagggcggc gacggttttg ggggcgagcg gcggtgtggt cagcatggtt
     3601 tatgcgccga aaaaccgttt tgccgcctca atcaggcgtg tgcatgaagt gccgtctgaa
     3661 aacgggtcgg caacgcagtc taaaggtgtt ttgcgcaacc aagtcagttg gcgtttggca
     3721 agttggcggg tggcggcaat gcctttctcg acaaatgccg ggaaatcggt ttttccgtcc
     3781 agatattccc atgcctgacg gtagccgacg cagcggatgg cgggggagtc ggcggtcagg
     3841 ccgggatagc ggcggcgcag gttttctact tcgccgataa agccctgttc aagcatcagg
     3901 tggaaacgca gggcgatgtt ttcatgcagg cgggcgcggt tttcgggaat cagggcggcg
     3961 gtatgcaaat cgaaggggag cgtatggggg ctttgtctgc cgaggtgttc gctcatcggt
     4021 ttgccggtca gataataaac ttccaaagcg cgtccgatac gctggctgtc gttcggtttc
     4081 agacggcatg ctgtttcggg atcgacctgt tgcagggtgc ggtagaggaa atccaagccg
     4141 tacatttgtt tttgttcgtc caagtcggca cgcaggcagg catcggcttc gggcaaatcg
     4201 ttcaaacctt gggtcagggc gcggaaatac atcatcgtgc cgccgacaat cagcggatat
     4261 ttcccacgcg ccgtgatttc gccaatcaga cgcgtgcagt cttcgacaaa acgggcggca
     4321 ctgtatgtct gaacaggcgt aatgatgtcg ataaggtggt gcgggacaaa ggcgcgttcg
     4381 gaggcggacg gtttcgccgt gccgatgtcc atatcgcggt aaaccagcgc ggaatcgagg
     4441 ctgatgattt cgacaggcag ggtttcggca attttgaggg cgagcgcggt tttgcctccg
     4501 gcggtcggcc cgagcagggc aaaggctttc ggggtcggca taacgtttca ggtttggaaa
     4561 aatacggatt atagcggaaa gcgtgccgac gttatatttt ggtttgcgga agcacgccga
     4621 cggcaagggg gcgtgtttac cgtatgcctt tatatagtgg attaacaaaa accagtacgg
     4681 tgttgcctcg ccttagctca aagagaa
//
LOCUS       DAWKOD010000149         4699 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000149 DAWKOD010000000
VERSION     DAWKOD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.149"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(416..952)
                     /locus_tag="WJK73_001757"
     CDS             complement(416..952)
                     /locus_tag="WJK73_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ7558200.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="WJK73_001758"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="WJK73_001758"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ7558201.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="WJK73_001759"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="WJK73_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ7558202.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2077..2922
                     /locus_tag="WJK73_001760"
     CDS             2077..2922
                     /locus_tag="WJK73_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ7558203.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3164..4138
                     /locus_tag="WJK73_001761"
     CDS             3164..4138
                     /locus_tag="WJK73_001761"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ7558204.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4297..4515)
                     /locus_tag="WJK73_001762"
     CDS             complement(4297..4515)
                     /locus_tag="WJK73_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ7558205.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1205 a         1389 c          997 g         1108 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataccgccac tgccgtacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgctcccgga tgacggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacaa
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagtg tttcgcatac ctcggcataa tgtaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacgccgat aagcgtatcc gccttgatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accacaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctacg
     1501 gatcgggcaa tcctggggcg accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgctgccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cccgcaactg ccttggtctg cgcgttggtt tggcttgtta
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccac aaaagtaata tcgtcaagct gctcgaaggc agagaaagca
     1981 aaatcggcgg cagccgctga tttcccatcc atccctgttt atgccgtctg aaccccgctt
     2041 cagacggcat ccgaatatcc cgccgtttta ccgactatgc tcagttacag acacgcattc
     2101 cacgccggca accacgccga tatgctcaaa cacttcacct tgtttttagt gctgcaatat
     2161 ttcaaccgca aagacaagcc ctactggtac atcgacacgc acggcggcgc gggtgtgtac
     2221 aatctcgaag gcagcgaggc acaaaaagtc ggcgaatacc ggcaaggcat cgccctactt
     2281 cgacaagcaa aaaacctgcc aaccgagtta aacggcttta tcgcgcacat acaaaaaatc
     2341 ctgccctcgc ccgaacttta ctgcggttct ccgtggctgg cgcaatcgct gacccgcgtc
     2401 ggcgacaaat tacgcctgtt tgagctgcac cccaccgact ttgtccatct gcaaaacaat
     2461 atgggcgaag caggattggg caaacgcgga caagtgttgc gcgaagacgg ttacaaaggg
     2521 ctgatttccc tgctgccgcc cccgccgcgc cgcgccaccg tcctcatcga cccgccctat
     2581 gaggaaaaac aggactaccg gcgtgtaacg gaaacgctga aagccgcttt aaaacggttt
     2641 gagtccggct gttatctcat ttggtatccc tgcctcagcc gcgaagaaag ccgcaagctg
     2701 cccgaagaat tgaaaaaact cctgcccgac aactaccttc acgccgaact tcacgtccac
     2761 gcgccgaaag ccgacggttt cggtatgcac ggcagcggaa tgttcgtcat caatccgccc
     2821 tatctgttgg cggaacaatt ggcggcaaac ctccccgccc tgacgcggct gctggcgcaa
     2881 gacgaaggcg cgcgctacct tttggacagc aaaatccgct gaccccgatg ccgtctgaaa
     2941 cccttcagac ggcatctccc atcaaacaaa accggacgat ataccggcag ccggcacttt
     3001 taagaataaa tatcccctca tccgcctgcc gcgttccgac ttgccccatg ccgttttttt
     3061 acactacaat cccctagact atcctgcaaa acgcaccaaa ggaatacgcc acaataacct
     3121 gccgcctttc agacggcaca aacctttccg aaacgagcaa actatggaca cactgacccg
     3181 attcctcccc gaacacctgc aacaaaacca gctgcccgaa gcactcggcg gcgttttact
     3241 gtccgttgtt tccgcctgca ccgaaatcaa cgccaaagtc cgcctcggcg cactggccgg
     3301 cgtattgggt atggcgggca cgggcaatat tcagggcgaa gaccaaaaaa aactggatgt
     3361 tatcgccaac aacatcatga ttgacacact caaagccaac cctgccgttg ccggtttggc
     3421 aagcgaggaa gaagactctt tcgtaagtgc cggtgaaaac ggacgctatc tcgtcctatt
     3481 cgatccttta gacggatcgt ccaatattga tgtcaacatt tccgtcggta cgattttctc
     3541 catccttgaa aaacccgaag gcgcattggc aaccgaatca ttccttcaaa cgggcagaca
     3601 gcagcttgcc gctggttacg ttctttacgg gcctcaaacc cagctcgtat tcacattcgg
     3661 acacggcgta tatatgttca cgctcaatgc cgaaaacgaa tttgtgctga caaaagaaaa
     3721 accgaaagta cccgaaagta ccaaagaatt tgccatcaat atgtccaacc gccgccactg
     3781 gctgcccccc gtccaacaat acatcgacga gctcttggcg ggcgaaaccg gcacgcgcgg
     3841 caaaaactac aatatgcgct gggtagccag catggttgcc gaaatccacc gcatactgat
     3901 gcgcgggggc gtgttcatgt atccgcaaga caaacgcgac ccgtccaaac ccggcaaact
     3961 gcgcctgatg tatgaagcca atcctatggc cctgatactc gaacaagccg gcgcatcggc
     4021 aagcaacgcc cgtcaggaca tactcggcat ccgtcccgaa agcctgcacc agcgcgtcgc
     4081 cgtcattatg ggcagcagcg aagaagtgga ttacctcaac cgcctgcatt caaaataaaa
     4141 ggcagcctga ccgataaaga aaggcggcag cgcgcatccg acgctgccgc cgatatttcg
     4201 tttatagcct taaaaagata gaaaaccacc tttccgaacg gcaaaaatgc cgtctgaaaa
     4261 gttttcagac ggcattttaa aaattaaagc ccgaatttat ttgcccgctg ctttttgcag
     4321 gatggcgtac aactcgtctt tcagtttcaa tttggctttt ttcagctcat cgatggtttc
     4381 cgcaccgctg gtaaccggat tgttgaccag accggtaatt ttatcgtcca gctcgttgtg
     4441 ttcgtcgaac agacgggcga agcgagaatt ttcctgtttc aatttggaaa tcaaatcacg
     4501 gtattctggg aacatatcgc tttcctctct tggtttacgg ttaaaaagtc aaattgaatc
     4561 tttacattaa ttataacaaa gataatattt ttatccagtg ttttatcgga tacggcaaaa
     4621 ttttctttta acaccgccgt ccggttgata aaaacgttga caatatcacg ctttacgcat
     4681 aaattttcag cctttccta
//
LOCUS       DAWKOD010000150         4606 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000150 DAWKOD010000000
VERSION     DAWKOD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.150"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(3..1496)
                     /gene="rng"
                     /locus_tag="WJK73_001763"
     CDS             complement(3..1496)
                     /gene="rng"
                     /locus_tag="WJK73_001763"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ7558206.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            1709..1966
                     /gene="grxC"
                     /locus_tag="WJK73_001764"
     CDS             1709..1966
                     /gene="grxC"
                     /locus_tag="WJK73_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ7558207.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            1987..2430
                     /gene="secB"
                     /locus_tag="WJK73_001765"
     CDS             1987..2430
                     /gene="secB"
                     /locus_tag="WJK73_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ7558208.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            2516..4558
                     /gene="recG"
                     /locus_tag="WJK73_001766"
     CDS             2516..4558
                     /gene="recG"
                     /locus_tag="WJK73_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ7558209.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGTQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1002 a         1292 c         1304 g         1008 t
ORIGIN      
        1 ttttacatca gcactatatc gtattgttcc tgcgtgtaag cggtttcgac tgccaaagaa
       61 atcggtttgc cgatgaaatc tatcagcatt gccaaggatt gcgattcttc gtccaaaaac
      121 aaatcgatga cgttgggggc ggcgaggatg cggaaacttt cggcatcgta acggcgcgct
      181 tcgcggacga tttcgcgctg gatttcgtag catacggttt gcggcgtttt cagacggcct
      241 ctgccttggc aggaagggca gggttcgcag aggacttggt ttaagttttc gcgcgagcgt
      301 ttgcgcgtca gctcgaccag tcccaggctg gtaaaaccgt gcagggtaac gcgggtacgg
      361 tcgaaggcga gggctttggc aagctcctgc aacacggctt cgcggtggct ttcctgcgcc
      421 atatcgatga agtcgatgat gatgatgccg ccgaggttgc gcagcctcaa ttcgcgggcg
      481 atggtgtggc aggcttcgag gttggtgcgg aaaatcgttt catcgaaatt gcgtgcgccg
      541 acgaagccgc cggtgttcac gtctatcgtg gtcatcgctt cggtggattc gataatcagg
      601 tagctgccga agttgaggtt gacgcgcggt tgcagggcgc ggctgatttc ctgttcgacg
      661 ttgtgggttt caaacagcgg gcgttcgcct ttgaacaatt ctatcctgcc caatgcgccg
      721 tggacgtatt gttcggcaaa acgcgtcatc cgtccgtggt ttacggtgga atcgacgagg
      781 attttctgcg tgtcgcagcc gaccatatcg cgcaatacgc gcaaactcaa gggcaaatcc
      841 tgataaagca gggtttccgg cggccggatt ttcgcctgtt cttggatgtg ttcccacact
      901 ttggtcaggt agtcgatgtc ggactggagc tgttcgtcgg tggcgttttc ggcgttggtg
      961 cggatgatgt agccccggca ggcattttcc ggcaggagct tgtcgaggcg ttcgcgcagg
     1021 ctgctgcgtt cggcatcgtc ttcgatgcgt tgggacacgc cgatgtggtc ttcttgcgga
     1081 agatggacga ggaaacgccc cgccagcgag atttgggtgg aaagccgcgc gcctttggtg
     1141 ttgatcgggt ctttgatgac ctgcaccaaa acagactgcc cttcaaacag catatgttcg
     1201 atgcgctggg tttcttcggg gttgcggcgt tgttcgagga catcgacgat gtgtaaaaac
     1261 gccgcgcgtt ccaagccgat gtcgataaac gcgctctgca tcccgggcag cacgcggcgc
     1321 accacgccca aatagatatt gccgaccagg ctgtgttcgc tgttgcgctc gatgtgcagc
     1381 tcgcagatat tgttttcctc caacaccgct acgcgcgttt cctgcggcgt gatgttgacc
     1441 aatatcgttt cgggcgggcg cgcgatgtct ttggggatgg ggagtcctga caacatggtt
     1501 tttcctgaaa atgtaataaa aatattttgc atccgttccg ccgcgcagcg cgggtgcaaa
     1561 aatatttttc acaatgtcta tcatacttta aaaaagaaac tttgacacat tccgcacatg
     1621 ccgtttgaac gtgcatctgt tccgcacttg ccaaacaggg gaaatgcccc tatattgatt
     1681 atcattgcaa aactttcgga aacccaatat gcagaccgtt accatgtaca caggtccgtt
     1741 ttgtccctac tgcgcgatgg cgaaaaggct gctgcacgcg gcaggtgtcg gacatatcga
     1801 cgaaatccgt gtcgatgcaa gccccgaagc ctttgccgaa atgcagcagc tttcgggaca
     1861 gcgcagcgtg ccgcagattt tcatcggcga aacgcacgtc ggcggattta ccgacctcta
     1921 ccgcctccag caggaaggcg ggctggacgg actgctgaac ccttaacccc caactaggaa
     1981 aacaaaatga gcgaagaact gcaacccgta ttcagcatcg agcgactgta tgtcaaagac
     2041 ttgtctttgg aagtgccgca cgcgccgcaa atctttttgg aacagggcga gcccgaagtg
     2101 gagatgcgcg tttccaccgg cagccaaaag ctggaagacg gctactacaa cgtggatgta
     2161 accgttaccg taaccgccaa attggataac gagcgcacga tgtttttgaa cgaagtaacc
     2221 caaagcggta ttttccgttt ggaaaacatc cccgaagaag atgccgacct gctgctcggc
     2281 gtggcgtgtc cgaacatcct cttcccttac gcgcgcgaag ccgtttccgg tacggtaacg
     2341 cgcgccggct tcccgcccgt cctgctcgcg ccgattaatt ttgaggcgat ttaccaacaa
     2401 cagcaggaag ccgaagccgc cggggcttga ttcctgcctg tcgatgccgt ctgaatccgt
     2461 ttcagacggc atttttgttt tcggataaaa taggcgcgtc caaaggaatc tgcctatgat
     2521 gtcgcccgaa acccgaaaac agctcaaaat caccgatgtt tccgccaaga agctcgacaa
     2581 actcaacctc cataccgcgt gggatttggt gttgcacctg ccgctgcgtt acgaggacga
     2641 gacgcacatt atgccgatta aggacgcgcc gattggcgtg ccgtgtcagg tcgaggggga
     2701 ggttatccat caggaagtaa cgttcaaacc gcgcaagcag ctgattgtcc aaatcgccga
     2761 cggctccggc agcgtccttt ttctgcgctt catccacttt tacgccagcc atcagaaaca
     2821 gacggcggtc ggcaaacgca tccgcgccgt gggcgaaatc aaacacggct tttacggcga
     2881 cgagatgatt catcccaaaa tccgcgatgc cgagggcggc ggtttggcgg aaagcctcac
     2941 gccggtttac ccgaccgtaa acggtttgaa ccagcccact ttgcgccgta ttattcagac
     3001 ggcgttggac gttacgccgc tgcacgacac gctgcccgat gccctgctgt gccgtctgaa
     3061 gctgccacac ctcgccgaaa gcctgcgtct tttgcattcg ccgccgccga gtttcacgat
     3121 tcatcagctt tcagacggca cgctgcctgc atggcaacgg ctcaaattcg atgaactttt
     3181 ggcgcaacag ctttcgatgc gcttggcgcg acagaagcgt atcggcggca cggcggcggc
     3241 attgggcggc gacggcacat tgacccaagc cctgcgccaa gccctgccgt ttgccctgac
     3301 cgatgcgcaa gaaaaagttg tttccgaaat ctgccgcgat atggcgcaaa cctacccgat
     3361 gcaccgcctg ctgcaaggcg atgtcggcag cggcaaaacc attgtcgccg ccttgtccgc
     3421 gctgaccgcc atcgaatccg gcacgcaggt ggctgtaatg gcgcccactg aaatccttgc
     3481 cgaacaacat tttattaaat ttaaacaatg gctcgaacct ttgggtattg aagttgtccg
     3541 cctttttggc agtttgcgta aaaaagccaa agacgaagcc aaagccaaac tcgccgacgg
     3601 cagcgtcaaa atcgccatcg gcacgcacgc cctgttttca gacggcgtgg cgtttcacaa
     3661 tttgggcttg accattgtgg acgaacagca ccgtttcggc gttgcccaac gcctcgccct
     3721 caaaaacaaa gggcgtgaag tccatcagct gatgatgtcc gccacgccca tcccgcgcac
     3781 gcttgcgatg agttttttcg ccgatttgga cgtgtccgtc atcgacgaat tgccgcccgg
     3841 gcgcaccccg attaaaacgc gcctcgtcaa caacgtccgc cgcgccgaag tcgaaggctt
     3901 cgtcctcggc acttgccgaa aagggcggca ggcgtattgg gtctgcccat tgattgaaga
     3961 aagcgaaacc ctgcaactgc aaaccgccgc cgaaaccctc gcccggcttc agacggcatt
     4021 gcccgaactc aatatcggac tggtacacgg gcgcatgaag gccgccgaaa aggccgaagt
     4081 catggcggaa tttgccgcag gccgtctgaa cgttttggtt gccaccaccg tcatcgaagt
     4141 cggcgtagat gtgcccaatg ccgccctgat ggtcatcgaa cacgccgagc gcatgggctt
     4201 ggcgcagctg caccaattac gcggtcgggt cgggcgcggc gcggcagaaa gcgtgtgcgt
     4261 cctcctgttt gccgaaccct tgggcgaact cgccaaagcg cggctgaaag tcatctacga
     4321 acacaccgac ggctttgaaa tcgcccgcca agacctcaat atccgcggcc cgggcgagtt
     4381 tctcggcgcg cgccaaagcg gcgtgcctat gctgcgtttc gccaaactcg aagaagacct
     4441 gcaccttttg gaacaggcgc gcgaaaccgc cccgatgctg attgagcaaa accccgaaat
     4501 cgtcgaagcg catttggcaa ggtggctttc cggcagggaa gggtatttgg gcgtgtgagc
     4561 aaaatgccgt ctgaaatatt agaaaaaaaa gtgtaacgga aatctg
//
LOCUS       DAWKOD010000151         4576 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000151 DAWKOD010000000
VERSION     DAWKOD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.151"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..915
                     /locus_tag="WJK73_001767"
     CDS             <1..915
                     /locus_tag="WJK73_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ7558210.1"
                     /translation="NTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGA
                     IGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQ
                     TAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLF
                     DETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMK
                     NNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNG
                     NFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            924..1301
                     /locus_tag="WJK73_001768"
     CDS             924..1301
                     /locus_tag="WJK73_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558211.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1579..1953
                     /locus_tag="WJK73_001769"
     CDS             1579..1953
                     /locus_tag="WJK73_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558212.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1988..3583
                     /locus_tag="WJK73_001770"
     CDS             1988..3583
                     /locus_tag="WJK73_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7558213.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGDVGKTLKELGRSRTVKKLVV
                     AAAIAGVSNKIGASSLATWSETPWVNNLNVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIV
                     GEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNN
                     ALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISR
                     GGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIK
                     HVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTK
                     GETKVRIVVQKGTNKIISAYPQK"
     gene            3602..3865
                     /locus_tag="WJK73_001771"
     CDS             3602..3865
                     /locus_tag="WJK73_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558214.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            3972..>4576
                     /locus_tag="WJK73_001772"
     CDS             3972..>4576
                     /locus_tag="WJK73_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7558215.1"
                     /translation="MQLLAAEGIHQHQLNVQKSTRFIGIKVGKSNYSKNELNETKLPV
                     RVIAQTAKTRSGWDTVLEGTEFKTTLSGADIQAGVGEKARADAKIILKGIVNRIQTEE
                     KLESNSTVWQKQAGSGSTVETLKLPSFEGPALPKLTAPGGYIADIPKGNLKTEIEKLA
                     KQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAA"
BASE COUNT         1488 a          862 c         1088 g         1138 t
ORIGIN      
        1 aatactgcgc atggagaggc agccagtaaa atcaaacagt tggatcagca ctacattgcc
       61 cataagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggcaagtgt
      121 caggatggtg cgataggcgc tgcagtcggt gagattgttg gtgaggcttt ggttaaaaat
      181 accgatttta gcgatatgac cccggaacaa ttagatctgg aagttaagaa aattaccgcc
      241 tatgccaaac ttgcggcagg tacagttgca ggcgtaacgg gaggagatgt caatactgct
      301 gcacaaaccg cacaaaacgc ggtagaaaat aatgcggtta aagctgttgt aactgctgca
      361 aaagtggttt ataaggtagc cagaaaagga ttaaaaaacg ggaaaatcaa cgttagagat
      421 ttaaaacaga cgttgaaaga cgaaggttat aatttagccg acaacctgac caccttattc
      481 gacgaaacat tggattggaa cgatgccaaa gccgttattg atattgtcgt cggaacagag
      541 ctgaatcgcg ctaataaagg ggaagcggca caaaaggtca aggaagtttt agaaaaaaat
      601 cgtccttata tccctaataa aggtgctgta ccgaatatga gtacatacat gaaaaataat
      661 ccttttggaa aacagctggc tcaaatttca gaaaagacaa cgcttccgac gcagcaaggg
      721 cagtctgtct tcttggtaaa aagaaaccaa gggttattaa aaaccggtga taggttttat
      781 ttagatggcc aacataaaaa tcatttagag gtttttgata aaaatgggaa ctttaagttt
      841 gttctaaata tggatggttc gcttaaccaa atgaaaactg gggcagcaaa aggtcgtaaa
      901 ttaaacttaa aataggaaat tttatggaaa cattgaatga tataaaaaaa atcttgatta
      961 atgtggggct ttatcaaggg tttgatttga cagatccaaa agtatcagaa gaagttaatc
     1021 atgaaacagc taatatgaaa tggattaaag attatacttc agacgggaat tgggataatg
     1081 aatttaagga ggatttaaaa aactttttag attatatgga agtatgccaa ttagccctaa
     1141 acgataaaaa tttcaaaatt gccagtaatt ctttatttat ggctatgatt tacgcaggta
     1201 atctatctct tatatttgat tcaataaaaa ctgatatatc aacattattg agtgctgagt
     1261 ataaaaagaa tagtttttca tggccatctc ttgatgaata gaaagcaagt tgtagcctgc
     1321 atgaaatcta aaacccatgc ataaggtgtg ggcttcagta tacgcgttcc atgatttacg
     1381 gccatatgcc gtctgaaaag ctcaattttt tcagacggca tttgttatga aagtaaatat
     1441 ttagattccc tgtatactgt ttagactcgt gtgtgctgag taagctgtag tctgcatgaa
     1501 acctaaaact cgctcaaaat taagctaaga cattagcagg gcaagggcga aaattgaatc
     1561 ttaaataagg tgattcagat gaaaaatttt aatgtagtaa aagaaagttt aagagagtta
     1621 ggaattaaac aaggatttga tctttatgag aaagccacaa ctgaaaaatt gaatagtgaa
     1681 gatcctcttg acttacaatg gctttctaac tattcatctg attggaatga tgaattagaa
     1741 gaagactttg attctttttt tcagcatatg aaggaatatc aatatgctat tgacaatgaa
     1801 gacattaaat ctgcatgtag ttcactatgt gaagctatgc tctatgttgg taatattaaa
     1861 aatttttttg agtttctcaa aagcgatatg attagactgt tgagaggtga aagtaaaaca
     1921 acagactttc aatggccgca atttgatgaa tagcagcaag ctgtagcctg catgaaacct
     1981 aaaatccatg cgtaaggtgt gtgcttcagc acgcacgcgt tccatgattt acggctcaat
     2041 gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa atattcagat
     2101 tccctatata ctgcccagat gcgtgcgtgc tgaagacacc ccctacgctt gctatttgaa
     2161 acagctccaa gtcaccaaag acgtcaactg gaaccaggta caactggcgt acgacaaatg
     2221 ggactataaa caggaaggct taaccggagc cggagcagcg attattgcgc tggctgttac
     2281 cgtggttact gcgggcgcgg gagccggagc cgcactgggc ttaaacggcg cggccgcagc
     2341 ggcaaccgat gccgcattcg cctcgctggc cagccaggct tccgtatcgc tcatcaacaa
     2401 taaaggcgat gtcggtaaaa ccctgaagga gctgggcaga agccgcacag tgaaaaaact
     2461 ggttgtagca gcggccatcg caggcgtatc caacaaaatc ggtgcttcct cccttgccac
     2521 ttggagcgaa accccttggg taaacaacct caacgtcaac ttggctaatg cgggcagtgc
     2581 cgcgctgatt aacaccgccg ttaacggcgg cagtctgaaa gacaatctgg aagcgaatat
     2641 ccttgcggct ttggtgaata ctgcgcatgg agaggcagca agtaaaatca aacaattgga
     2701 tcagcactac attgcccata agattgccca tgccatagcg ggctgtgcgg cagcggcggc
     2761 gaataagggc aagtgtcagg acggcgcgat tggtgcggtc gtgggggaga ttgtcgggga
     2821 ggctttggtt aagaataccg atttcagcgg tatgactgct tctgaaattg aaaaagctaa
     2881 agcgaatatt actgcgtatg caaaattggt agccggagcg actgtaggtg ttacaggagg
     2941 caatgttgat gtggcggcaa atgcttccga aacagctgtt aaaaataatg cattagatat
     3001 tatttgggat atcggcaacc tcgtatggga cggcggtaaa tggatttacg ccaaatctat
     3061 tggcgataag cagatggctc gagaagcggc gattgatttt ggtgtggatg ccgccgcagc
     3121 tgccgttccc tttgttccgg caggtgcgac taaaatcagc cgaggcgggg cttatgttct
     3181 gaaggcggga gacgaagcag ttgatacggc taaagccata caggaaattc agaagcagac
     3241 cggaatcaag cttacttatg ataaggttaa taaggtttgg acaacaccgg cggggttaga
     3301 ttatgggtta gatgctaagc atggtaatag gattaaacat gttttagccc atacaattcc
     3361 aaatccaaac aaacctgttc attctgtttt taatgtgtcc cgtaaagaag ttttgccttt
     3421 ggttgatgaa gcttggagaa tgaaaggaaa tcctttgcca aatgattcat ccgtatatct
     3481 tgtagatatg aagaaaccta ttggaacaaa aggagaaaca aaagtgcgga ttgttgtgca
     3541 aaaaggaaca aataaaatca tttctgcata tcctcagaaa taattaagaa aggaatctct
     3601 tatggataaa gaaattaaaa tttgcccaag atgtgagcaa ggctaccttt atcatgcaaa
     3661 gcctaaatat ttctctgggg aggtcatttt atgcgatgaa tgttatgcta tgtggcttgg
     3721 ggatatgaaa attttttacg gacaatatgg aaaagatttt tatgattatc atgagtttat
     3781 gaaagataaa ggcatagaag aaataaatat gtgggaagga gagctttttg atcacccata
     3841 ttatgaggat gaaaaattta aataattgat tttctgttcc ccgaatttgg gaaatacgat
     3901 gatattttaa acccaaatat tatttaaagt agcaataggc cgtctgaata tccgtttttc
     3961 agacggcctc aatgcaactg ctggcagccg aaggcattca ccaacaccaa ttgaatgttc
     4021 agaaaagtac ccgtttcatc ggcatcaaag tgggtaaaag caattacagc aaaaacgagc
     4081 tgaacgaaac caaactgccc gtacgcgtta tcgcccaaac agccaaaacc cgttccggct
     4141 gggataccgt actcgaaggc accgaattca aaaccaccct ttccggagcc gacatacagg
     4201 caggggtggg tgaaaaagcc cgagccgatg cgaaaattat cctaaaaggc atcgttaacc
     4261 gcatccaaac cgaagaaaag ctggaatcca actcgaccgt atggcaaaag caggccggaa
     4321 gcggcagcac ggttgaaacg ctgaagctac cgagctttga agggccggca ctgcctaagc
     4381 tgaccgcacc cggcggctat atcgccgaca tccccaaagg caacctcaaa accgaaatcg
     4441 aaaagctggc caaacagccc gaatatgcct atctgaaaca gctccaagta gcgaaaaaca
     4501 tcaactggaa tcaggtgcag cttgcttacg acagatggga ctacaaacag gagggcttaa
     4561 ccgaagcagg tgcggc
//
LOCUS       DAWKOD010000152         4575 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000152 DAWKOD010000000
VERSION     DAWKOD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.152"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..1851)
                     /locus_tag="WJK73_001773"
     CDS             complement(58..1851)
                     /locus_tag="WJK73_001773"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ7558216.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2097..3206
                     /gene="proB"
                     /locus_tag="WJK73_001774"
     CDS             2097..3206
                     /gene="proB"
                     /locus_tag="WJK73_001774"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ7558217.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3219..4481
                     /locus_tag="WJK73_001775"
     CDS             3219..4481
                     /locus_tag="WJK73_001775"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ7558218.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLEQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1050 a         1243 c         1270 g         1012 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagct tgctcaaagc
      121 ggaaaggtag gctttggcgg tggcgaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt
      241 gacggcattg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 aatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 acgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaaatctcaa taaaccgcca tagattttat taattttata
     1981 cattttgtcc attttattgc agacgcggcg tgattgttga caattgcggc agaacagggc
     2041 aaattcggcg gcacaaggtg gcaacgagac aggacaaacc aacaaggacg gaaacaatga
     2101 aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg gacggcagtc
     2161 tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat catgcgggac
     2221 acgagctggt cttggtgtct tccggcgcgg ttgcggcagg gtttggtgcg ctgggtttca
     2281 aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg caggggctgc
     2341 tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgcg caaatcctgc
     2401 tcagccgcgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc gcactttccg
     2461 tgctgctgca acgccgcgcc gtccccatca tcaatgaaaa cgatacggtt tcggttgagg
     2521 aattgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg atacaggcag
     2581 acctcttggt gctgctgacc gacatagacg gtctttacac gggcaacccg aacagcaatc
     2641 ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc gaaatggcgg
     2701 gcggatcggg ttcggcaaac ggcacgggcg gtatgctgac taaaatcaaa gcggcgacga
     2761 ttgcgaccga gtccggcgta ccggtctata tctgttcctc gctcaaaccc gatgcacttg
     2821 ccgaagcggc agataatcag gcggacggct cgtttttcgt cccccgtgcc aaaggtttgc
     2881 ggacgcagaa gcaatggctg gcgttctatt ccgaaagcag gggcggcgtt tatgtggacg
     2941 aaggtgcgga acacgctttg tccgaacagg gaaaaagcct gctgatgtcg ggcattgccg
     3001 gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag gcaaccaaac
     3061 agcctttggg caaagggcgc gtcctgttcg gctctgccgc cgccgaagac ctgctcaaat
     3121 tgcgtaaggc gaaaggcgtg ttcatccatc gggacgactg gatttccatc acgcccgaaa
     3181 tacgcctgct tctgaccgaa ttttagaaaa gaaaacccat gtcaaacaca caaaaacagc
     3241 ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa gaaaaaaacc
     3301 gcgcgctgtt tgctatggcg gacagcttgg aagcggcggc ggcagatatt ttggcggcaa
     3361 accgtcaaga cttggaagcc gcggcaggca aaattcccga aagcatgact gaccgccttt
     3421 tgttggacgg caaacgcatt tgcgcgatgg cggacggcat cagggcggtt gccgcactgc
     3481 ccgaccccgt gggcgaaata ctggaaacct cgactctgcc caacggcttg gaaatcgtca
     3541 aaaagcgtgt ggcgatgggc gttatcggca ttatttacga aagccgcccg aacgttacgt
     3601 ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc agcggcaaag
     3661 atgcattcca atccgcacgc gccatcgttg ccgcactcaa agccggtttg gagcaaaccc
     3721 gcatcgaccc cgacgcgctc cagttgattg aagacacagg gcgtgagggc agttacgaaa
     3781 tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggcg gggctgatac
     3841 gggcggtggt tgaaaatgcc gtcgtgccgg tcattgaaac gggaacgggc attgtccaca
     3901 tttatatcga caaggacgcg gattgggaca aggcgctccg tatcgtgtac aacgccaaaa
     3961 ccagccgtcc gtccgtgtgc aactcgatgg aagtgctgct ggtacatgaa gacattgctg
     4021 ccgacttcct gcccaagctc gaacggctgt tggttcgcga ccgcatagaa gccggactgc
     4081 cgcccgtccg cttccgtttg gatccgcagg cggcgcggca tatcggcggc gaagcggcgg
     4141 gtgcagacga tttcgatacc gagtttttag actacatcct cgctgtgaaa accgtcgctt
     4201 cggtcgaaga ggcggtctgg cacatcgaaa cccacagcac acaccattca gacggcatcg
     4261 ttaccgaaaa ccgccacgct gcggactatt tcacgactca tatcgattct gccgccgtgt
     4321 atgtcaacgc gtccacgcgc tttaccgacg gcggcgaatt cggcttgggt tgcgaaatgg
     4381 gcatctccac gcaaaaactc cacgcccggg gcccgatggg tttgaaagag ctgacgagtt
     4441 acaaatacat cgtacaaggc acgggacagg tcagggaata atccgaattc aaatgtctga
     4501 tatagtggat taactttaaa ccggtacggc gttgcctcgc cttagctcaa agagaacgat
     4561 tctctaaggt gctga
//
LOCUS       DAWKOD010000153         4565 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000153 DAWKOD010000000
VERSION     DAWKOD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.153"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(272..379)
                     /locus_tag="WJK73_001776"
                     /pseudo
     CDS             complement(272..379)
                     /locus_tag="WJK73_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJK73_001777"
     CDS             complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJK73_001777"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ7558219.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1177..1968)
                     /gene="recO"
                     /locus_tag="WJK73_001778"
     CDS             complement(1177..1968)
                     /gene="recO"
                     /locus_tag="WJK73_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ7558220.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWGGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2002..3129)
                     /gene="pheA"
                     /locus_tag="WJK73_001779"
     CDS             complement(2002..3129)
                     /gene="pheA"
                     /locus_tag="WJK73_001779"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ7558221.1"
                     /translation="MLECTINYCSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3166..4398)
                     /locus_tag="WJK73_001780"
     CDS             complement(3166..4398)
                     /locus_tag="WJK73_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ7558222.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         1009 a         1309 c         1260 g          987 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tattacataa tcatcgcctc aatatttaat
       61 atcccaaaat atagatttaa aaccatcgcc tttgtggttt gtcagcattc ctaattgaga
      121 tagaaagtca aataatttct tgtttctccg gctgccatca agatggttta gattaagacc
      181 gcgaacaata caataaccaa tacgctgtac tgtattgtga atttcttcta tcaaactatt
      241 attgtttgca gataaaatat acttttgatt catatagtga attaaattta aaccagtacg
      301 gcgttggctt gccttgccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
      361 ttaaatttaa tccactataa catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct
      421 gcccttacgg cagcagcctt gccctgaaca tcgcctcctt catctggcgc acggcttcgg
      481 gcagtccgag gaagagggct tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg
      541 cgaggatttg ggcgatgggg gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga
      601 ccaagcccaa atcgccggca aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt
      661 gttcggcgtg gctgcgcgcg tcggcatacg cgccggtgtg cagctcgaca acgggcgcgc
      721 cgacatcata ggcggcttgg atttgcctgt cgtcggcatc gataaagaga gagactcgga
      781 tgcctgcgtc ggtcaggatt ttggtgaacc cggcgatttt ttcctgttgc gccaatacgt
      841 ccaagccgcc ttcggtcgtg atttcctgac gtttttcagg cacgatgcac acgtcttccg
      901 gcatcacttt aagcgcgttt tcgagcattt cttccgtcaa cgccatttca aggttcaggc
      961 gcgtgcggat ggcgtttttg acggcaaaca catccgcgtc tttgatgtgg cggcggtctt
     1021 cgcgcaggtg catggtaatc aaatccgcac cgtgcgtttc ggcaaccagt gccgcctcca
     1081 cggggctggg ataagtcgtg ccgcgcgcat tgcggacggt agcgatgtgg tcgatgttga
     1141 cacctaaaag cataatcttt ccttttattt ctgccttcag acggcatttg aagccgtgcc
     1201 gtccgaagtc gggacggttt cccgggcggt ttctttgcgg tcaaactgcc gtatctgttc
     1261 caacacctgc cgcgatttca gcccctcggg caacaggtgg cggataaaaa gccgtgtgat
     1321 tttcaatgcc tgttgcaggc tttcggcggt gcggaaactg ccttcgcgca aatcgatcag
     1381 gctttgccct tcgacaattg cgccggcttc gtgcgaaagc gcgtctgcgt cctcgcatac
     1441 gggcatgacc gcctcttcgg gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc
     1501 cgtgccgtcg gcgtgcaaat cgggggcaac gcccaaggca ttgagcagct tccactcgaa
     1561 gcggcgcaaa tcggcaatat ggttggcttc acggcacacc gcctccatca cttttgccaa
     1621 cgcgtcgtac agttcgggca tcggatcttc gcgcgcggtc agtttcaaca ccagttcgtt
     1681 cacatacaga ccgctgaaca acgccctgcc ctgcggctgc cgccatccgc cgccccattc
     1741 ggcacggtga agggttttga gttcctgact gccgtaccac gacgcactga cgggcacgaa
     1801 aggaaccagc acgccgcgca attcgctctg ccgtttgcgc gcgcttcttg ccaaaagtgc
     1861 cacacgcccg taacggcggc tgaacgcttc gacccgcagg ctgctttcgc gccagggcga
     1921 ggaagccaac aggaagacgg gttcgtggtt gactcggtgt tcggacatca tgcctgaaat
     1981 gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga tgactttgac
     2041 gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt cccggcggtg
     2101 tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttactcg gacggctctc
     2161 aaacttggtc atggaaatac ccgactcagt cagcggttgc agcagcgagg caaccgcgcc
     2221 tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg caccggtttc
     2281 gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga tgcactcggc
     2341 aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg caaccgtacc
     2401 gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg caatccgttc
     2461 ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct gcgcgtggga
     2521 aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt tgtggtggat
     2581 gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt ctaacgtgcg
     2641 accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg cctgacgcgt
     2701 ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg cggcatgacc
     2761 gaaatgctta atcgccgcct gttgggtaaa cgtgccctgc ggccccagat aggcgatggt
     2821 cagcgggcgt tctacggcga ggcactcgct catcacttcc cgaaacaggc gtgctaccga
     2881 ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg cgacttcgcg
     2941 ttcgggacgg tacactgcgc ccgtgccttt cagttcgccg atggcgtgtg cgtgttgcgc
     3001 gcgttcgttg agcaggcgca ggatttccgc atcaatggtg tcgatggcgt tgcggtgcgg
     3061 gatgaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat ttatcgtgca
     3121 ttctaacatc aaatgccgtc tgaagcggtt tcagacggca tcggtttaca agtattcgct
     3181 ctgttcgttt tccttccacg cccggtgcga acacagccac agaagcgcaa tgccgcaaga
     3241 ggtggacgcg gtcatcgttg ccgccatcac ggttgccgaa ccgtcgtgca agaaggtcgc
     3301 cgccataccc acccccgcgc cgattaagga ctggaacacg cccaacacgg cgttcgcgct
     3361 gccgccctct tctttgaaat aagacataaa gcatgcctgc gtgttcgcgc cgaccaagcc
     3421 ctgcgtgccg acggaaaaca tcacgcacgc aaccaagagc cagaacggag gcaacccgaa
     3481 aaacagcacg gcggcgagtt gggacaggtt ggcggcaaat tggacgacaa tcccccacag
     3541 caggatgctt tgcggatgcg cgccggtttt gagccgccac gcggtaatgc ggttgaaaaa
     3601 catcatcgtg atgatgttga gtgcaaacgc ccaagcgtat tgatgaggcg taacacggta
     3661 aagctgctgg tacacgaagg aagattcggt cagaaaggcg aacatcgaac cgaagctgaa
     3721 tgcctgaaaa aacagataac ccatcgcggc acgggttttc aatacgcgtt tgaaccgccc
     3781 agccaccagc ccgaacacat cccgtccgat tttgccgccg acggcaggct tgggcaggaa
     3841 atactgcacc aaaccgagca gcaccagcga atacgccgcc agaaaaacaa aaatcgcctg
     3901 ccagccaccc aagccctgca acagtgcgcc gaccatgggt gcgaccagcg gcacaaccat
     3961 caaaatgatg ccgataaggg caaacatctg ggcggctttg cgtccggaat aataatcgcg
     4021 caccattgcg ccgacgatga ccacagtcat gcccgcaccg aatgcctgca cgacgcgcag
     4081 gttgaggagc tgttcggcac tcgaagcaaa tacgatggcg gcaacggcaa ggcagtagac
     4141 ggccagtccg gtcagcgcga cgggtttgcg ccctttgatg tcggacaccg aaccgccgac
     4201 cacctgtccg aacgccgtgc cgaacataaa caaactcagg ctctgttcga tgcggtggac
     4261 atccgcgttc agcgactgcg ccatttcggg aatcgcgggc aggtaggcat cgatggaaaa
     4321 cggcatcagc gtaaccagca tcgccatcaa aaccgccatc agtttttcgc tcatttcagg
     4381 ataatgggca gaaggcataa attctcgtcc gattcgcaaa caaaccgccc ggcggcggaa
     4441 aacacaatac gcggaaacgg aaaattttaa cacaccggca caaacaggcg gcaacaaaag
     4501 ccccggcaaa accggacgaa ccggaacgat atagtggatt aacaaaaacc agtacagcgt
     4561 tgcct
//
LOCUS       DAWKOD010000154         4543 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000154 DAWKOD010000000
VERSION     DAWKOD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.154"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1036..1806)
                     /gene="xth"
                     /locus_tag="WJK73_001781"
     CDS             complement(1036..1806)
                     /gene="xth"
                     /locus_tag="WJK73_001781"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7558223.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1866..2147)
                     /locus_tag="WJK73_001782"
     CDS             complement(1866..2147)
                     /locus_tag="WJK73_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ7558224.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAAAILRTIRDLENKRAV"
     gene            2249..2482
                     /locus_tag="WJK73_001783"
     CDS             2249..2482
                     /locus_tag="WJK73_001783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558225.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2615..3496)
                     /gene="nadC"
                     /locus_tag="WJK73_001784"
     CDS             complement(2615..3496)
                     /gene="nadC"
                     /locus_tag="WJK73_001784"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ7558226.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3572..4510)
                     /locus_tag="WJK73_001785"
     CDS             complement(3572..4510)
                     /locus_tag="WJK73_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558227.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1043 a         1143 c         1211 g         1146 t
ORIGIN      
        1 cgacttgcgt ttcatgaaac tgatgcagat atagtggatt aacaaaaatc aggacaaggc
       61 gacgaagccg cagacagtac aaatagtacg gcaacgctgt actggtttaa atttaatcca
      121 ctatattttg taatagctgc cattatgcct actaggatat ataccttcta tataatcctt
      181 atttaaataa aaacccttat aaaaatctgt gttatctgtc gggattgaaa aaaagatttt
      241 atctttctca tcaggtttat atttgctatc aatatcaaaa agagaataag gattacaatg
      301 agttcgacat actaggtaaa aatttatatc ttggatcatg aaatattcga tatttatcag
      361 gatcgaaaga tgctaaaaat tcatcaatat cggtactgga tattttgtca tcactaataa
      421 acatcgctag agatgggcga ctttgatttt ccatttgttg attagtaaag gatataaata
      481 ctaacttgcc attgtcaaac cacatagatt tatatagtgg attaaattta aaccagtacg
      541 tcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
      601 ttttgttaat ccactatagt tattgccttt gggagaatca attctgacag cacataacat
      661 aggttcatct ttacagtcgt tatattgggt ttcatcaaac tcaactgcat agcacaataa
      721 aggcaatagg aaaaaaagga gattaattta ttactataca taaggcagtc tgactattca
      781 ttctaaaata aatccggttt ttaatatttg attctgctcc ggattatata cataataaat
      841 tttactgctc caaaatctga tattaaccct atggatttca taaatttttc agaaaaatag
      901 gctgtgcttg aacaatccgc cgactccggc tgtttgtcag ggaaaaggca attattttcg
      961 gttagtcagc agcctatcct attgaatcac ttaaagatat ggatgtttgt caacgccata
     1021 tcaaaacacg gtcttttacc aatcgaattc tgccgtcacc ggcgcgtggt cgctcggacg
     1081 ctccagcgcg cgcgtctcca aatcgacacg gacatccttc aacgcctccg ccatcgcagg
     1141 cgacaccaaa atatggtcga tacgcaggcc cagtttgcgt tggaacatcg cgccgcgata
     1201 gtcgaaccag gtatagaacg cgccttcggg atggacttgg cgcaggctgt cggtcagtcc
     1261 caaatccagc aggtttttga accactgccg ttcgacggac gaacagtgga ttttttcgtg
     1321 ccatttttca gggtcgtaac agtccgcatc ggcaggcgcg atattgaaat cgcccagtag
     1381 caccagtttg ccgtagcggg tcatttcatc gcggacaaat tcggttaacg cggcaaacca
     1441 ttgttcttta tatttgaatt tcgggctgtc gagggcttcg ccgttgacgc aatagacatt
     1501 gatgacgcgc acaccgccga cggttgccgc aatcacgcgc cgttgcggat cgtccggcag
     1561 cgcgggcagc ccgatatgca cgtcttccgg cgcgttgcgg ctgacgattg ccacgccgtt
     1621 gtaggttttc tgcccgctcc aaacacagtg ccagcccatc atttgcaaag cggcggccgg
     1681 aaatttgtcc tgatcgagtt tgagttcctg caaaaccaaa atatcaggcg gattgtcggc
     1741 aagcaggttt tgcacctgcg gcagccgcac attgagcgaa ttgacgttcc aagtggtgat
     1801 tttcataaca tttccgaaaa atgccgtctg aaacgttcag acggcaaaag gaaaggattt
     1861 taacactata ccgcgcgttt gttttccaaa tcgcggattg tccgcagaat cgccgccgcg
     1921 tcttcggaaa tcaggcggta ttcgataatg cggtggtaac gcgtaaagtc caccagaccg
     1981 cccgccctca ggcggttcaa atggccggaa acggcggtgg ccggcaggga taaggatttt
     2041 gccagttcgg cgatattgcg ttcgctgtcc aacagttgga tcagtatcgc catacgttcg
     2101 ggatgcgcca taagtttgag tatggtgtgg agcggtattg tgttcatcgg tattcctaaa
     2161 acctttgccg cccgccaagc ggattgacgg gcgacagata tggatgattg tgattatctt
     2221 tataaatcgt attataaatc aaatatttat ggatgattgt acaggattgt atgttccttt
     2281 tcccactttt tttgcaaaca cgcgtaaagg cgggctttca gacggcataa aactgccgcc
     2341 cctaccctgc ctcaccctgt tgccgaacgg aatcatccgc attgttcaaa agatgcctta
     2401 caaccgccgc acgtcttgcc ctgccgatgc ctccgtgcct tcgggcaaac aaaatacctg
     2461 cctgaaacgc tgttttttct aaagcatcac gaccttcacg aaaaatgccg tctgaagaat
     2521 atttcagacg gcatttcctc cttgaggcaa taaccggatt acggttaggc ggttccttgt
     2581 ctgacatatc agacaactgc cgacacccta aaactcatgc caagaaatcc aaacctatgt
     2641 ccaacgagcg gctgctgtgg gtcagatagc cgagggcgat gccgtccacg ccggtttgcg
     2701 ccacgcgctt caggcggtcg aagccgatgc cgcccgatgc ttcgcaatag acggtgtggg
     2761 ggtgggcggt ttgcgtgtgg cagcggtttg ccgcttcttt cagggtttcg tcgtccatgt
     2821 tgtccagcaa aatccgttcc gcgcccgctg cgatggcttc gtccagttgt gccaacgtgt
     2881 ccacttcgat ttccacgcag gtcaatgctc cgaccgcctg ttttgcctgc cgaacggctt
     2941 gggcgatgct gccgcaatag gcgaggtggt tgtctttgat gagcacggcg tcgtccaagc
     3001 ccatgcggtg gttcacgccg ccgcctgccc tgacggcgta tttttgcagg acgcgcagca
     3061 gggggatggt tttgcggctg cacacgatgt ctgtaccgta ttcggcgact tcggcaacgg
     3121 cacgcgcggt ggcggtggcg atgccgctta agtgcgtgag gtagttgagt gcggtgcgtt
     3181 cggcggcgag cagcgcgcag gcgttgcctt cgacgacggc cagcgtctga cctgcgcgga
     3241 cggcttgtcc gtcttggatt tcggcttgga aacggacgga cggatccatc gtctgaaagg
     3301 cgagacacgc caagtccata ccggcgataa caccgtcttc gcggctgacg aggaagagtt
     3361 tggcggtttt gtcgggggcg ataacggcgg cggacgtaat gtcgccgcgc ctgcccaagt
     3421 cttcgctcaa ggcttgttcg actatggggc gcaacagggt gtcgggcagg gaaaagaggg
     3481 tgttttcaga cggcatgagg tttcctttgt cgaaaaagat tgatgttgca ccgatggggc
     3541 aaaacttcat ttgatgccgt ctgaaaacgg gctaacggct gcccagcggc agtccgatgt
     3601 ccgaaatcag cctgtcgggc aggacgtcgt cgcggaagcg gcaaagctgc gcgggacggc
     3661 ctttgctgcc cgatacgccg gtatccgacg gctcgatgag gttttgctgc tgaatctggc
     3721 ggcggaagtt ttgcttgtgc agcaatcttc cgctgatggc ttcgacactg ttttgcagtt
     3781 gcagcagcgt gaattcgggc ggcatcagct caaaaatcac ggggcggtat ttgattttgg
     3841 cgcgcaggcg ggacagggcg gtcgccagta cgcgacggtg atcgtggcgc atgggctgcc
     3901 ccgtgagcgc gaagtcgaag tttgcctgcg gctcggcggc ttccactatc agaccgcttt
     3961 catacagcat ttcatagcgt tgtaaaacgt attcttccga ccagttttcc ggttcgaccc
     4021 cccagcacaa atgaatgcgc ttgagccgca tttgacgcac ttcctccgtg tccgccgaat
     4081 ttgcccaaat gcgcaggcgg ctgacgacgg cgtcgcgctg cccgccgtcg gtgcgcaagt
     4141 cttcccacgg gaaatagccg tagcagtcct gccatttcgc atccgggtgc aggatgctgt
     4201 cggctgcctc gcgcaccagc cccaaatagc tgacgtacaa gacgggcatg ccgtgttcgt
     4261 tgcggcggtg ggtatcgaca aaggtgtaaa gctgttccac atagcccata ggctgcgaag
     4321 tctgcttggc aacccacagc ttcacgcccg cctgcaggga attgcgcaga ggggagagcg
     4381 ggccgttggg caggagtgtg ccttgggcga cggttaatac ccgcaggccg ccgtcggtaa
     4441 cggcaatcaa tacgggaacc agctcgacga tgctttgcgg cggggcttcg gcttcggggt
     4501 aggcatccat gctcggcttt ctgcgggtgg gaaaggcttt att
//
LOCUS       DAWKOD010000155         4445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000155 DAWKOD010000000
VERSION     DAWKOD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.155"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..1205)
                     /locus_tag="WJK73_001786"
     CDS             complement(63..1205)
                     /locus_tag="WJK73_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ7558228.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WJK73_001787"
     CDS             complement(1254..1676)
                     /locus_tag="WJK73_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558229.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WJK73_001788"
     CDS             complement(1669..2478)
                     /locus_tag="WJK73_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7558230.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WJK73_001789"
     CDS             complement(2560..2937)
                     /locus_tag="WJK73_001789"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ7558231.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WJK73_001790"
     CDS             complement(3137..3904)
                     /locus_tag="WJK73_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7558232.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="WJK73_001791"
     CDS             complement(3914..>4445)
                     /locus_tag="WJK73_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7558233.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1078 a         1108 c         1101 g         1158 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt acccaactgc
      241 ggataaagcg cctgcaaagc gtggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggtcgg acggactgcg cgtcggcaac gccgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcggg tttgcgcttc ggcgcggcgg
      481 cgcaaatccg cccacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaagcggcgc
      541 atcatgcccg gtgcgcggcc tgtggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggaggctttt gacgttggca
      901 cgctttgcct cgattttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gctttcgagt
     1141 tcttgcgata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcatagcgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccggcggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 agcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 gaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAWKOD010000156         4441 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000156 DAWKOD010000000
VERSION     DAWKOD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.156"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..170)
                     /locus_tag="WJK73_001792"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="WJK73_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(242..487)
                     /locus_tag="WJK73_001793"
     CDS             complement(242..487)
                     /locus_tag="WJK73_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558234.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(488..784)
                     /locus_tag="WJK73_001794"
     CDS             complement(488..784)
                     /locus_tag="WJK73_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558235.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(775..1128)
                     /locus_tag="WJK73_001795"
     CDS             complement(775..1128)
                     /locus_tag="WJK73_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558236.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1364..2791
                     /locus_tag="WJK73_001796"
     CDS             1364..2791
                     /locus_tag="WJK73_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ7558237.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2860..4263
                     /locus_tag="WJK73_001797"
     CDS             2860..4263
                     /locus_tag="WJK73_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ7558238.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
BASE COUNT         1119 a         1096 c         1167 g         1059 t
ORIGIN      
        1 tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt ttcggtttct gtaccccccc
       61 ctgttagata aggggggaag gtttgaagcg gttgccggct tcctgccgtc cgctggcgcg
      121 tccgtcatca cgccggcaac cgcctttgtc atcccttgct tatcttccat ggtgcgaatc
      181 ctcaaaaacg ggcaaaaaaa aagccctgtt acttgtagaa agtaaaggac gttaattttt
      241 gttaatcgtc ccttcttagg gacgcaatat ataagggacg caatatataa ggttttaaaa
      301 cgctaagaac aaaatccgat aacggctctg aagaagtaag tctttcttta ttgcacaaga
      361 tagcaaactt ccacggcatt ctcgccccac ccgtgccgtc caagcctttt tcaaagacat
      421 agatggtttt cttggcaatc acttcgtcag tttggcggtc gataatccgt atggtcgcac
      481 ctgccactca gtgcctgcgc agcttggaat cgacattgtt ttcgaacgtt accgcataac
      541 gggcgaggtg tatatgatta agtatttgat ttatcggaaa atctttacct gaataccgaa
      601 taatatcgga atggttgggt tgcaggacat ccacatagcg gtattcccca tcgctaaaaa
      661 atcctaggaa acgagcaata aaatttactc cttcgctggt ctgtaaagcc gcattgtccc
      721 acatcgggtc tctggttttt gcatctgccg aaacggtacg ctcaggtact accttcaaca
      781 gcataatccc ttccacattg tccgccgtct ggtaaatctt tcccccgccg ttttgcattg
      841 ttcgttaaac acggcttcgg cttctttgta ttttctgtcc cactcttctt gtgcctgtat
      901 ttcttctttg atcgggccga attgtttggg aataatccaa acaaacagca tcaggatagc
      961 ggcggcggtc aggctgcctg aaaggatttt gccggggttc cgtttgggct ttttataggc
     1021 aaagcggacg agaaaccaaa gcaacagcag catggtgccc caatagccga ttgagaatag
     1081 gatggccaaa ccttctagga aatggcgtaa atcgtttgtg gtaaacatgg gttgttcctg
     1141 tggttaaatg tgcaggctgc tttttgccga atcttgccgc atctcaaaag cagcctgcgc
     1201 ttcagcgttg cgttacgcag taaaataatg aatatttgta acggcttggg tattttttgt
     1261 caatattccc gcctttccct taacagctgc cgcgctttcc gttaaaattc ctttacatat
     1321 ttatattgtt tcctgtttct atattgccaa ggttatactc gttatgtttt tctccgccct
     1381 gaaatccttt ctttcccgct acattaccgt atggcgcaat gtttgggcgg tgcgcgacca
     1441 gttggaaccg cccaaacgca cggcggaaga acaggcgttt ttgcccgcgc atttggaact
     1501 gaccgatacg ccggtctctg ccgctccgaa atgggcggcg cgttttatta tggcgtttgc
     1561 gcttttggct ttgttgtggt cctggttcgg caaaatcgat attgtggcgg cggcttcggg
     1621 caaaacggtg tcgggcgggc gcagcaaaac catccagccg ctggaaacgg cggtggttaa
     1681 ggcggtacat gtgcgcgacg ggcagcatgt gaaacaggga gaaacgctgg cggaactgga
     1741 ggctgtggga acagacagcg atgtggtgca gtcggagcag gctttgcagg ctgcccaatt
     1801 gtccaaactg cgttatgaag cggtattggc ggcattggaa agccgtaccg tgccgcatat
     1861 cgatatggcg caagcacggt ctttaggtct ctccgatgcc gatgtgcaat cggcgcaggt
     1921 gttggcgcag caccagtatc aggcatgggc ggcgcaggat gcgcaattgc agtcggcttt
     1981 gcgcggccat caggcggaat tgcagtcggc caaggcgcag gagcagaagc tggtttcggt
     2041 gggggcgatc gagcagcaga aaacagcaga ctaccgccgt ttgcgggccg acaattttat
     2101 ttcggaacat gcgtttttgg agcagcagag caaatcggtc agcaattgga acgatttgga
     2161 aagtacgcgc ggtcagatga ggcagattca ggcggccatt gcacaggcgg agcagaatcg
     2221 ggtgctgaat acgcagaacc tgaaacgcga tacgctggat gcgctgcgcc aggcaaacga
     2281 acagattgac caataccgcg gccaaacgga taaggcaaag cagcggcagc agctgatgac
     2341 aatacagtcg cctgcggacg gcacggtgca ggaattggcc acctatacgg tgggcggtgt
     2401 ggtgcaggct gcccaaaaaa tgatggtgat tgcgcccgat gacgacaaaa tggacgtgga
     2461 agttttggta ttgaacaaag acatcggttt tgtggaacag ggacaggatg cggtggtgaa
     2521 gattgagagt tttccctata cgcgctacgg ttatctgacg ggcaaggtga aaagtgtcag
     2581 ccatgatgcg gtaagccacg aacagttggg cttggtttat acggcggtgg tgtcgctgga
     2641 caaacatacc ttgaatattg acggcaaagc agtgaatctg acggcgggca tgaatgtcac
     2701 ggcggagatt aaaacgggta aacggcgggt gctggattat ctgttaagcc cgctgcaaac
     2761 caaattggac gaaagcttta gggagcgata ggcggatccg tactgggcat ttgttatccg
     2821 ccggttcgga catgcagact gcctgaaacc attgcccgga tgacattgct caatctaatg
     2881 ataatgcaag attacggtat ttccgtttgc ctgacactga cgccctattt gcaacatgaa
     2941 ctattttcgg ctatgaaatc ctatttttcc aaatatatcc tacccgtttc actttttacc
     3001 ttgccactat ccctttcccc atccgtttcg gcttttacgc tgcctgaagc atggcgggcg
     3061 gcgcagcaac attcggctga ttttcaagcg tcccattacc agcgtgatgc agtgcgcgca
     3121 cggcaacaac aagccaaggc cgcattcctt ccccatgtat ccgccaatgc cagctaccag
     3181 cgccagccgc catcgatttc ttccacccgc gaaacacagg gatggagcgt gcaggtggga
     3241 caaaccttat ttgacgctgc caaatttgca caataccgcc aaagcaggtt cgatacgcag
     3301 gctgcagaac agcgtttcga tgcggcacgc gaagaattgc tgttgaaagt tgccgaaagt
     3361 tatttcaacg ttttactcag ccgagacacc gttgccgccc atgcggcgga aaaagaggct
     3421 tatgcccagc aggtaaggca ggcgcaggct ttattcaata aaggtgctgc caccgcgctg
     3481 gatattcacg aagccaaagc cggttacgac aatgccctgg cccaagaaat cgccgtattg
     3541 gctgagaaac aaacctatga aaaccagttg aacgactaca ccgacctgga tagcaaacaa
     3601 atcgaggcca tagataccgc caacctgttg gcacgctatc tgcccaagct ggaacgttac
     3661 agtctggatg aatggcagcg cattgcctta tccaacaatc atgaataccg gatgcagcag
     3721 cttgccctgc aaagcagcgg acaggcgctt cgggcagcac agaacagccg ctatcccacc
     3781 gtttctgccc atgtcggcta tcagaataac ctctacactt catctgcgca gaataatgac
     3841 taccactatc ggggcaaagg gatgagcgtc ggcgtacagt tgaatttgcc gctttatacc
     3901 ggcggagaat tgtcgggcaa aatccatgaa gccgaagcgc aatacggggc cgccgaagca
     3961 cagctgaccg caaccgagcg gcacatcaaa ctcgccgtac gccaggctta taccgaaagc
     4021 ggtgcggcgc gttaccaaat catggcgcaa gaacgggttt tggaaagcag ccgtttgaaa
     4081 ctgaaatcga ccgaaaccgg ccaacaatac ggcatccgca accggctgga agtaatacgg
     4141 gcgcggcagg aagtcgccca agcagaacag aaactggctc aagcacggta taaattcatg
     4201 ctggcttatt tgcgcttggt gaaagagagc gggttagggt tggaaacggt atttgcggaa
     4261 taaagcaggc tgaaacggtt atgaaattcc caaagcagcc tgcaccccgt ttcgaaagtg
     4321 caggctgctt tgggattgat ccgatacttt cacattctca ttgcctttaa ataaagcaca
     4381 gttaaaaaca catttaaatt aagtgcgaat taattcaaat gtatattcaa ttaaaatcaa
     4441 a
//
LOCUS       DAWKOD010000157         4385 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000157 DAWKOD010000000
VERSION     DAWKOD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.157"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(45..3077)
                     /locus_tag="WJK73_001798"
     CDS             complement(45..3077)
                     /locus_tag="WJK73_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ7558239.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3242..3922
                     /locus_tag="WJK73_001799"
     CDS             3242..3922
                     /locus_tag="WJK73_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ7558240.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3951..4310
                     /locus_tag="WJK73_001800"
     CDS             3951..4310
                     /locus_tag="WJK73_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ7558241.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          885 a         1286 c         1125 g         1089 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc
      181 gaaatgctgg ctttgcgcgt tttcaggaca acggatacgg cctcgtcgta catcggatcg
      241 gtttcgtcgt cgccgctgcg cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagga taagatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac caccaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cgggttgccg attttctccc cccttgctgc ggcttcggcg
      781 attttttgat tgaaacccgc aagattgcgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccagcaaatg cggtgctttg
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccga ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgga taccgcaccg gtttcttcag atgggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacattttc aaacggacaa
     1621 accgcctgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gtttgaccgc
     1681 tctgccgcac cttcactgcc gtcatctgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcagcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgtcg gcggctcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcagcag gttcttcaat atagatatcg gcagcttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcaggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgtccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     2281 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     2341 tctttgtccg catcgaacat acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     2401 ataatctgta gcccttccat cggggtatcg gacacgcgga ctttggggac cgctgtttcg
     2461 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gctgtttaaa
     2521 gcacgcgttg cttcttccaa agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg
     2581 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     2641 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgcttct
     2701 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcggtcgga
     2761 acatccgcac ggtttgcaga agcaggcgga acgggcctgt ccggttcgga ttcgtgcgcc
     2821 agatagtttc ggcagaaacg gacgacaccg acaaacagcc aatacactgc cgttttgacg
     2881 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     2941 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     3001 cgttcggatt gtgcgcggac aaaaaacaag ccggcaagcg caagcaacaa aataacgatc
     3061 aaaactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     3121 acgtgtttcc atcccaaaaa aaacaaccgg tcggcaattc tgccgaaacc gattcggctt
     3181 tcggtttgcc cccgccgaca gtataatagc cgtcctttcc cttaatcttt gcggcattgc
     3241 catgattttc caaacagtct ggttttcaga tatggtactg tcggtttcgt ggattgtcct
     3301 tatcctgata ctggcagctt ccgcgccgtc cgcattccgc tcgcttgccc gctaccgttc
     3361 cgcccttccc ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     3421 cggcggacag cttgcccaga tgaactacca tctgctcgcc gtcaatctgg ttgcactgat
     3481 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     3541 gtttgccggt tctgccggcg catatccgcc gaacgctttg gtgctgattc tgcccgcgct
     3601 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     3661 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct
     3721 gaccggtatt ttggatgctg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     3781 gcccgtcttt attctgctgg cgtgggcgga agccttcctc agcggcattt caactgccat
     3841 atttgttgca ctgaagcccc attggatcaa cactttcgac gacaaccgct atctgaagtc
     3901 cgaccgcggc atatggcggt agccttcaga cggcataggc agacaaaacc atgctttcca
     3961 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     4021 tcgccgttcc tgccgccatg tctcccgcca cgggcaacct gtttgccaac tggacgggcg
     4081 cgctgctcat cggcatcttt gccgaaacca tcaatcaccc gcaatggaag ctgctgctga
     4141 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     4201 tgctccaatc gaatcgtcct gcttcagcac ttgccaatat tttcctacac acggcaggtt
     4261 cgctgctgct gacttggctc gggctgaaga taggggcggc agtcaaataa ccgcaacaaa
     4321 ttgcctccct ttgttttgac ataatctata aattagcttc gcttgtttaa atactggctg
     4381 tgcca
//
LOCUS       DAWKOD010000158         4370 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000158 DAWKOD010000000
VERSION     DAWKOD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.158"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..2045)
                     /locus_tag="WJK73_001801"
     CDS             complement(69..2045)
                     /locus_tag="WJK73_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ7558242.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVAKNA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WJK73_001802"
     CDS             2194..3033
                     /locus_tag="WJK73_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ7558243.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDESACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WJK73_001803"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WJK73_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7558244.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGPLKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1102 a         1476 c         1011 g          781 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cgaccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc atatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcccaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccaaac cggccgcctc caacacttca
      661 cgccttttcg catcgccgaa cgacaccggc tcgccggcat ttttggcaac ctgcacgcgc
      721 gcgatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagtgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatta atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggcc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggcgc aaccgtcgtc
     2341 cgcgaccttg ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga atccgcctgt atcggctgca ccgcctgtat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ccgacatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacaaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccacc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 accagcaaag aaaaagccaa aatccacctc gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcgc gacaccacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcccgctc aaaggcgttt tgggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacactacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat ggtcaaagtg ttcgcttggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaacaa accgtcaaac aaaatgccat ctgaaacccg atgttttcaa gtttcagacg
     4321 gcatttttca ttttcaccgt gattttatcc ggctgtcgtc atttctaatt
//
LOCUS       DAWKOD010000159         4319 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000159 DAWKOD010000000
VERSION     DAWKOD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.159"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..180
                     /locus_tag="WJK73_001804"
     CDS             <1..180
                     /locus_tag="WJK73_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ7558245.1"
                     /translation="NENTNGLIRQYFPKQTDFRNISDREIRRVQDELNHRPRKTLGYE
                     TPSVLFLNLFQPLIH"
     gene            complement(441..1634)
                     /locus_tag="WJK73_001805"
     CDS             complement(441..1634)
                     /locus_tag="WJK73_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ7558246.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1631..2599)
                     /locus_tag="WJK73_001806"
     CDS             complement(1631..2599)
                     /locus_tag="WJK73_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ7558247.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAVPQTGETEWKQD
                     TEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWP
                     KTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVERILEAAH
                     THRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGEDRYTDSV
                     NVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2610..4046)
                     /locus_tag="WJK73_001807"
     CDS             complement(2610..4046)
                     /locus_tag="WJK73_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ7558248.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(4176..>4319)
                     /locus_tag="WJK73_001808"
     CDS             complement(4176..>4319)
                     /locus_tag="WJK73_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ7558249.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT          920 a         1095 c         1371 g          933 t
ORIGIN      
        1 aatgagaaca ccaacggact catccggcaa tacttcccca aacaaaccga tttccgtaac
       61 atcagtgatc gggagatacg cagggttcaa gatgagttga accaccgacc aagaaaaaca
      121 cttggctacg aaacgccaag tgttttattc ttgaatctgt tccaaccact aatacactag
      181 tgttgcactt gaaatccgaa tccaaggccg tctgaaaacg gaaggcggca gtttatcatc
      241 tttgccttca gacggcatat caaacgccgc ctttgcgccg tatgccggag gttcgccccc
      301 gtccttccaa tgcttgaggc gaatgccggt tgcgtccggc aaaccgtgcg cggggtggct
      361 atgccgtctg aagcaatatg aaggtatgcg tttcagacgg catccccctg ttgcggaaat
      421 accgtccgcc tcctgtccga ttattgcctg attgcagcgg cgcggacgag ttcttcgagg
      481 ctgtcggcga gcatttccgc cgcgcgccgg tagcctttgg cggagaagtg tacgccgtct
      541 ttggcggccc atccttggtt gagccagttt ttcatgctgc atatgccgcc catggcgttt
      601 tgccaagacc agaacatggt ttgtccctgc cgggcgacgc gccgctgcat atgttggact
      661 tcggtcaggc ggacggggcg tgtgccgcat acgccgagcg tgtttttcag ggattcgggt
      721 gcgccgatga tgaggatgcc ggcggcaggc aggctgtcgt ggatttggcg gacggtatcc
      781 agccattttt gttcagtgtc ggcaatgtcg atgttgtcgc cgaaggcttc gttggtgccg
      841 taggaaagga taaccaaatc ggcgccggtt tgggcaaggt cgttcatacg gtcggcacgc
      901 catttcgacc aatgggtcaa ttgtgcgccg ttgatgccca tggcggaaac ggtaatgccg
      961 ccggcgggat tttcgatgtt gatgaagccg atgtcccacg gcatttcggt ctgtatggtc
     1021 aagggcagtg ccgcgcgggt atccagtacc tgccagccgc cgccgttggc ggagacggtg
     1081 ttgccgttga cggtcagggt ttgttcggcg agcaggggtt tggcaaacag ggaaatgcgc
     1141 tgtttgccgg tcttgccgtc cgatgcggtc agggtcatgc tgccgccgct gccggtgtgg
     1201 gcgaggatgc cgccgagcgg gaagtctccg ctattgttcc tgctggtgag gctttgccag
     1261 ttaccgttgt gccggacggc cgccatgcgc tgccctttga cgttggcggg gtaaacccag
     1321 cctatgccgc cgtcgcccca agttttttgc aggcgtttgc gcaggctgtc ggtaaagaag
     1381 tcgccggcgg tatgcgagtc gccgatttgc aggatgcgga aggtctcgcc gctgccttgt
     1441 gcgacggatt ggagtttttt catccaaggc gaggcggcgg cgttgccgta gtcggtcagc
     1501 agcccgtttg tatcggtgta gggggcggac ggggaaacgg taacggtgtc gagactgacg
     1561 gaggcaacag gtagggcgtt tgcctgggtt gcggcgaaca gggcggaaaa tgcgatgagg
     1621 tgtttggggt tcatggctgt gtacttgatg gttgcgtact tggttcaaaa acgatttttt
     1681 ccattatttt tgccgccagc agtttttgtc cttcggcggt aaagtgtatg ccgtccttgc
     1741 tgcggtagcg gacgggtttg ccgttgacgt tgacggagtc cgtgtagcgg tcttccccgc
     1801 cgctcagggt gtgcgcggtg ggaatcagga tgattttgcc tttcaaatgt tccgaaagca
     1861 gtttgtcgag gtagcgcatc tgcccgtcaa gcttggcttt tttcatgtag gggatgccga
     1921 gccagacgac ttggacgcgg tgcgtgtgtg cggcttcaag gatgcgctcg acgcgtttca
     1981 ggtattcttg cgcccattcg tcggaagcaa atttgaggta gcgtttgccg acgggaaaat
     2041 cccacgggtc gttcgggccg aggaagacgg cgagtacgct gatttcggga tgtttttgca
     2101 gggtttcttc aatcgttttc ggccagtcga agaatgaggg gtaggacagc cccgtgcttt
     2161 gtttgctgag gttgacggat tcgatgccgt attgctgttt caggcttttt tgcacgaagg
     2221 gtgcaacgcc ctgcatcagc gagtcgccgg cgaaaaagac tttgtcgccg ctgcggacgg
     2281 cggcggcttc ggtgtcttgt ttccattctg tttcaccggt ttgagggacg gcttcagatg
     2341 gcatatctgc cgagccgccg tcttgagccg tcggcggcgt ttcgccggac aggaatgttt
     2401 tgatgccgtc tgaaaaggcg taggcgtttt cttgcaacgc cgcaccgctc cgccaccatc
     2461 cgtaggcggc aagcggttcg agcggactgt tgcggtggta ggtctgctgc cagtaggcgt
     2521 tgatggggtt ttggctgaac cacacggcaa tcagggcaga catcaatatg gaagtgaaaa
     2581 gggaaagaaa gtgtttcatg tccaaaccct taaaaattgg cataaataaa gccgggaatc
     2641 cccgaggggg cgaatacgat aatcagcagc aggacggcgg aaacggggat aaaccacagc
     2701 cacatcggga ttttttccaa acctttgacc gcgccgtcga aagcgcgttg caggtaaggg
     2761 tagagcagca tcacggatgc aaacgaggca agcagcagca tatccgcccg ttgcggcgca
     2821 ttccagccgt tggcattggc aaagagggca ttgaaaactg cgcctgcatc gtcggggttt
     2881 gcggtattga agacgacgaa ggtcaggcag acgaaatgga aggtaacaaa ccacgagagc
     2941 ggcgcaaggt atttcagacg gcatagtgcg ttgcgtccga aatagcggtc gcccgtgttg
     3001 agcagcacca gcgccgtgcc gtgcagcgcg ccccaaatga ggaagttcca gccgtagccg
     3061 tgccagatgc ctgagagtac cattgccgcc atcaggttga gctgtgtccg taaaaagcct
     3121 tttttgctgc cgcccaaggg gatgtagatg tagtcgcgta tccaggtgga aaggctgatg
     3181 tgccatttgt cccaaaatgc acggatattc gcggcacgaa gcggtgctga gaagtttttg
     3241 ggcagtcgga agcccagcag cattgccatg ccgataacca aatcggaata tccggaaaag
     3301 tccaggaaga gttggaaggt atagccgtac acgccgccca atacgcccca gccgtcgaat
     3361 tgggcggggt tttcaaatac gggcgatacc cagttttccg ccagcatccc cgccagccac
     3421 cattttttgg caatacccag cagaatcagg gaaacggcga gtgcgggacg gacgggcgaa
     3481 cgcggttggc gggtacggat ttgcgccaat gcgcccgcct gttcgccgtc cgcgcttttg
     3541 aatgcggcgg cgcggataat cgggccggag gtaacggtgg ggaaaaaact caggtgcagc
     3601 agcagttcgt gccactcgaa acgcgcggcg tgcggcgcac ggaagcagta aaccaaatag
     3661 gcgattgact ggaaggtgta atacgaaagc cccagcggca tcaggatgtc gattgcgccg
     3721 ccttgtccgg catattgtgc aatcagcggg cggaaaaagt cgaaatattt gaaaaagccc
     3781 aagacggtca gcgagacggc aatgccgcac cccagccaaa agcggcgcgt attttcgcga
     3841 tcggaacgga gcagttcgcc caaaaggtac acgcaggagg aataaaggac gacgattgcc
     3901 gcaaatacgg ggccgatatg gtagagccag cccataccgg cagccaaaag cagcaggttt
     3961 tggacggacg ggtatttcgc caagccccag taaatcggca ggaaggcgag aaagaacagt
     4021 gcgaactcga cagacagcag cggcataaac catccttgga ataagaaaat aggcgttaaa
     4081 acacggcaaa gcccgttatt ttaagcaggg gcggatattt cttcaatcaa aatgccgtct
     4141 gaagggcggg aggggcttca gacggcatcg cgcggtcagg tttggatatg gcggttcaag
     4201 ttcaggcaca gccacagccc ggccgccgcc gcaagcatac cggcgatgcc gatacgggaa
     4261 atgccgacat attgcgttac ccaatgtccc aacagtgcgc cgctgccgat accgacgtt
//
LOCUS       DAWKOD010000160         4307 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000160 DAWKOD010000000
VERSION     DAWKOD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.160"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(153..398)
                     /locus_tag="WJK73_001809"
     CDS             complement(153..398)
                     /locus_tag="WJK73_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558250.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="WJK73_001810"
     CDS             510..938
                     /locus_tag="WJK73_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ7558251.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="WJK73_001811"
     CDS             950..1426
                     /locus_tag="WJK73_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ7558252.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="WJK73_001812"
     CDS             complement(1776..2264)
                     /locus_tag="WJK73_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ7558253.1"
                     /translation="MLKITLIAACAENLCIGADNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYYAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WJK73_001813"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WJK73_001813"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ7558254.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
     gene            complement(3549..3776)
                     /locus_tag="WJK73_001814"
     CDS             complement(3549..3776)
                     /locus_tag="WJK73_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558255.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4003..>4307
                     /locus_tag="WJK73_001815"
     CDS             4003..>4307
                     /locus_tag="WJK73_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558256.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         1019 a         1115 c         1011 g         1162 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaacctct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgtattgc
     1081 cggacaacgg gcgtggctga cgctgaaagg ctatatttcg gaactcagcc gcagtgagtt
     1141 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gtccgtttaa
     1201 gatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ctatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaagcg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagctcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgt aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgtc cgccccgatg
     2221 cacaggtttt ccgcacatgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cggcatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctttt gccgtacact tccggcttgt cctgcctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaata tcttgtgcca cttccatctg ccgggtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt cccgcatcat aattcggctc ttttccgccg cgcaaaatga catgacagtc
     2701 aggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg caatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatggtttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttca tagattttaa atttcatatt tttaatattt
     3541 taggaatttt attccaaaga aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 cggacaaaac cgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 cccgctgccg attccgcctg cacatccggc aaaatttcat acgcaaggaa aatgttgaaa
     3721 acaaaaagaa ctaattgctt gaaaaaagat ttaattggaa tgcgggtatt tcccacactt
     3781 tctccattta ttcatcctca ttataggaag aaaaacatga aaaaaccctg cttgcactct
     3841 cacttgcagc cgtgtccgcc gttacggtag ccgatattcc tgagtctaac ggcaagggca
     3901 aagccatcat cggcgataag gaaaaacacc gttaccaagc atgaaaacct tgttttgaaa
     3961 gtctatggcg aaatccgcca agatttgggg gacggatgat ccctgacccc ttgggcggat
     4021 gcatatgcag atttgcgcgg caaaaccaaa gtgatgacga cccagatgtg tgcttcccgc
     4081 gatgtcagca aaagcgccaa aggttggagt gtcggtatcg gtctgaatgt aggcaaacag
     4141 ttgaccgaca gcgtcggtct cgagtttgat ccatactacc gtcacaaaac aatctgcaaa
     4201 ccccgtgaga ttgttttgga cggcgacaaa accaaaatgg gccgctccaa atccaacgag
     4261 tacggcttcc gcgtaaccgc aacgttctat agtcaattaa aatcaaa
//
LOCUS       DAWKOD010000161         4300 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000161 DAWKOD010000000
VERSION     DAWKOD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.161"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..761
                     /gene="mafB"
                     /locus_tag="WJK73_001816"
     CDS             <1..761
                     /gene="mafB"
                     /locus_tag="WJK73_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7558257.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFSAAYNTRT
                     TRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHL
                     FPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYET
                     RDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            776..1006
                     /locus_tag="WJK73_001817"
     CDS             776..1006
                     /locus_tag="WJK73_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7558258.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1155..1862
                     /locus_tag="WJK73_001818"
     CDS             1155..1862
                     /locus_tag="WJK73_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7558259.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            1865..2188
                     /locus_tag="WJK73_001819"
     CDS             1865..2188
                     /locus_tag="WJK73_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ7558260.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2247..2474
                     /locus_tag="WJK73_001820"
     CDS             2247..2474
                     /locus_tag="WJK73_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558261.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2601..3143
                     /locus_tag="WJK73_001821"
     CDS             2601..3143
                     /locus_tag="WJK73_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7558262.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKYNLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            3278..3955
                     /locus_tag="WJK73_001822"
     CDS             3278..3955
                     /locus_tag="WJK73_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ7558263.1"
                     /translation="MVKTADGYKAIAHIQTGEYVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLVSNKIHPFYSQGKWIQADRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVANWHTYFVKSSQAETEGVWVHNDCSSRKTPSTPIYEDDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            3970..>4300
                     /locus_tag="WJK73_001823"
     CDS             3970..>4300
                     /locus_tag="WJK73_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7558264.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFT"
BASE COUNT         1466 a          803 c          904 g         1127 t
ORIGIN      
        1 tgggcatagg cgacatactg tacggaacgc gctatgccat agacaaagcc gcaatgcgca
       61 acatcgcccc cttgcccgcc gagggcaaat tcgccgtcat cggcggcttg ggcagcgtgg
      121 cgggctttga aaagaatacg cgcgaagccg ttgaccggtg gatacaggaa aaccccaatg
      181 ccgccgaaac cgtcgaagcc gtcttcaacg ttgccgccgc cgccaaagtc gcgaagttgg
      241 caaaggcggc aaaaccgggg aaggctgcgg ttagcgggga tttttctgct gcatacaata
      301 caagaacaac tagaaaagtt actacagaaa cagaggggtt aaatagaatc agacagaacc
      361 agaaaaatag taatatacat gagaaaaatt atggaagaga taatcctaat catattaatg
      421 ttttatctgg aaattctata caacatatac tgtatggaga tgaagcagga ggtgggcatc
      481 tttttcctgg caaacctggt aagacaacat tcccccaaca ttggtcagcc agtaaaataa
      541 ctcatgaaat tagtgatatc gttacatccc caaaaacgca atggtatgca cagactggaa
      601 caggcggcaa atatattgct aaaggaagac cagccaggtg ggtatcatat gaaacgagag
      661 atggaattcg tatcagaaca gtttatgaac ctgcaacagg aaaagtggta actgcattcc
      721 ccgatagaac ctctaatccc aaatataacc ctgtaaaata atgagtattt gaaagatgaa
      781 aacattagac gaacgaataa aaaatttagg aaaatctctt gaagatagaa ttgacgctaa
      841 tctaattgat gccgcactag agtacattac tttttcagaa cgtttgctag cttttgaaac
      901 gctatgtgat tatatagaag atttcaatat ccagcttacg gaaaaagaat cccaagaaat
      961 ctcttttatt aataaggaat ttggaattga aagtacatca gattaataaa tattaggtcg
     1021 gtttaaaaac ccaacaatca aaaagtcgtc tgaaactgtg tttaatttac agacgacctt
     1081 tccttcattt gaagcaggat attgagaact gagttcttca aaaatcctac acctgctcct
     1141 tccacggcag caccttggtc aaaacggcag acggctacaa agccattgcc cgtatccgag
     1201 tcggcgaccg cgtcttcgcc aaagatgaaa ccagtggaaa aactggatac aaacctgtta
     1261 ccgcccgata cggcaatccg taccaggaaa ccgtttacat tgaaatttca gacggcatcg
     1321 gtaacagcca aaccctgatt tccaaccgca tccatccgtt ttacagtcaa ggaaaatgga
     1381 tacaggcagg tcgtctgaaa aaaggcgaca ccctgctttc cgaaagcggt gcaaaacaga
     1441 cagttcaaaa cattaccctc aaacagcagc cgctcaaagc ctacaacctg accgttgccg
     1501 actggcatac ttatttcgtc aagggtaatc aggcggaaac ggaaggggtt tgggttcata
     1561 atgattgtcc gtatggagaa aaatatagga cagaagttgg ttcttatact aatactcatg
     1621 aaagtggtcg aacttattct ggaaagggca caagacaacg ttcccaaatc tctggagcaa
     1681 gagaagctag ggttaataat gaccctcata tagctacaga ttttactcct gccaaaaata
     1741 atcgagaggc attcaaagat gaatctagaa gaattgacgc tcacggtggt tccaaaagta
     1801 ccaataatta caatcgaatt gaaagcccgg ggaaaaaata ccgaaaacaa gatggagatt
     1861 aaaaatgatt gttgacgaaa aatctacttc agatgtactg aaacaatatc aagaactagg
     1921 gtttgatttg acaaaaccta tgataataga gttttttata ggaggttctc aaaagaatct
     1981 gcaatcaata gaaagccgac tcatttctta tcggcaagat tttcaaatca gtattgaaca
     2041 agatgaattc ggagaaatgt ggacttgcta ttgttccgtc aatatcgtcc cttcccttga
     2101 aaacatttta gctattgaaa ctacgttatt tggtattgca caaaaaaatg attgtaatta
     2161 tgaaggtttt ggttcgtatg ggaactaaca aaaagtgtag gttgggttga aaaaaccata
     2221 ttcccattaa agattagggg atgtctatgg aaataaaatt caatactttg ggggttatcc
     2281 tgaatggggt caatcctgaa gaaaaattca taaaaattat tgatgaccag gaaaataccg
     2341 gtggatttct gatactgtta tcttcaaatg acaaattctc atcatttgac agttacgatg
     2401 actgggtcga aaacttagaa atattaaaag aatacctgca agaatctcat tggataataa
     2461 agtgggtcgg atagtcggca aacgtagtcc gtctgaaacg cttttacttt tcagacggac
     2521 tttctatcaa tggggacaag gcgttttgtc tcaatctggt ctatgattat tcgatacagt
     2581 gatgggaagc taagaaaaat atgattaatc atattgaaaa atatacaggc actatttctc
     2641 atggctcgaa aagtgattcg ggaagtcagt acaaattaaa tatcgcagct attccctctt
     2701 cccctaacag ggatttaaaa acttatatta ctttagggtt aagcaaatat aatctgaatt
     2761 ataaaagcag gtttgaaata ctttttgtat gttctttaaa atatgatgaa aatcagattt
     2821 ttccattttt aagatggttg gcagaaacca tcattgagaa caaaaaaatt cttcttcgag
     2881 gacaggttat ttatttgcct agaagcattg ttgattcaac aaaaatggat gcactgtatg
     2941 tttccgcccc attttatttc gatgatgatt ttcaggtctg ttatggcgaa cactacaata
     3001 tcgttttccc tttgcttgtc cccttgtata aacaggaagc cgaattggtg gaaaagaaag
     3061 gttggaatgc ttttgagcag ttcttgctgg ataatgaagt tgacaacctt tcggatatga
     3121 ataggaaacc gtttgtttgg taagggtttt cttaaatttg attaaaggtc gtctgaaatt
     3181 aagcttgttt ttcagacggt ctttccttca tttgaaatag gatattgaga actaagttct
     3241 tcaaaaatcc tacacctgct ccttccacgg cagcaccttg gtcaaaacgg cagacggcta
     3301 caaagccatc gcacatatcc aaactgggga gtacgtcttt gccaaggacg aagcaagcgg
     3361 aaaaacggga tacaaacccg ttaccgcccg atacggcaat ccgtaccaag aaaccgttta
     3421 cattaaagtt tcagacggca tcggcaacag ccaaaccctt gtttccaata aaatccaccc
     3481 gttttacagt caaggaaaat ggatacaggc agatcgtctg aaaaaaggcg acaccctgct
     3541 ttccgaaagc ggcgcaaaac agacggttca aaacattacc ctcaaacagc agccgctcaa
     3601 agcctacaac ctgaccgtcg ccaactggca tacctacttc gtcaagagca gtcaggcgga
     3661 aacggaaggg gtttgggttc ataatgattg ctcgtcaaga aaaacaccat ctacccccat
     3721 atatgaggat gattcagaag catatgcagc agcaaagaaa ttgggatata ggaaaataaa
     3781 agaaagaacg agaaatgatg ctgctatttt taaaaaagga aagagttata tatcacgcga
     3841 tgtagattca cataatggag gggcttggaa agaagcatct tcacccaaaa atctgaacag
     3901 aaaagaaaca agaaacggta catttgataa aaacctaaat cgtattggag attaattatt
     3961 atgtgtatta tgtgtgagtt aaaaaacttc agaagaaata ttacttgttt tgagggatat
     4021 gacgaaaatt catttattgg caaatggtat gatgacgggg tgtgggatga tgaagaatat
     4081 tggaagctgg agaatgattt aatcgaggtt aggagaaaat atccttatcc gatggatata
     4141 ccaagggaca tcgtgattgg aatcggtacc attattgatt ttttaatggt tcaaaattgg
     4201 aaactttttg aaattaaagc ttctccttgg ttgcctaaaa gtgtcaaaat taatgagcgt
     4261 tatgaaaggt tcagagtaat gctccgttat atttttactg
//
LOCUS       DAWKOD010000162         4272 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000162 DAWKOD010000000
VERSION     DAWKOD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.162"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..1119)
                     /gene="mltB"
                     /locus_tag="WJK73_001824"
     CDS             complement(10..1119)
                     /gene="mltB"
                     /locus_tag="WJK73_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ7558265.1"
                     /translation="MKNRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASIANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1028..1279)
                     /locus_tag="WJK73_001825"
     CDS             complement(1028..1279)
                     /locus_tag="WJK73_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558266.1"
                     /translation="MPSDGIFGGGIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKQKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1321..2748)
                     /locus_tag="WJK73_001826"
     CDS             complement(1321..2748)
                     /locus_tag="WJK73_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7558267.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYTLENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKIFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2933..>4272
                     /locus_tag="WJK73_001827"
     CDS             2933..>4272
                     /locus_tag="WJK73_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7558268.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKQLKVVLTQDAE
                     QALRLQTAWLFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAP
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADIFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSEEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVT"
BASE COUNT          890 a         1191 c         1184 g         1007 t
ORIGIN      
        1 taaaaaacat tacaatcccg ggccgccaag cgaattggca atgtccctga ccgccgttac
       61 atacatccga ctgtgattgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
      121 ttcaaacacg ccgggtgcgg tttccagttt gaacaaaacc gccttttcat catcggcaag
      181 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
      241 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagtat
      301 tttcccgccc gtgcgccaac cgtgctgctt catataattg gcaatcgatg ccgcgacatc
      361 gccaacattg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
      421 gtagctcgaa ggcataaatt gcggcatccc cattgcgccc gcatagctgc ctttaaaggc
      481 gaaaacatcg ccgccttctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
      541 aaacccggcg cggcgggggt aatcaaagcc taaggtcgcc aatgcgtccg ccacacggaa
      601 actgcccgta tttttgccgt aattcgtttc aatcccgata accgccacga taagttcggc
      661 aggcacgccg tatttttgcg ccacatcatc gataagcgcg cggttttccg cataaaaccg
      721 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
      781 cgatgtggag gggcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
      841 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
      901 gcggacattt gcattggcgg caaacccgct gtcggatacc ggtacggctg ccgcgtcaaa
      961 tacggctgcc gcgtcaaacg cggggcggct ttcttttttc atttcatccg cgcggggggc
     1021 ttgggcttca tttgcccggg gcgggcgtgc ctccatcgcc gtacaggcag acaaagccgc
     1081 caaacaaatt gccagcggca gtatttttct gtttttcata aatatgttcc gaacaaatag
     1141 ggtaagtggg aaagcggcac aagggcgcgg cgcgaaatgc ggcatccgcc gcaatcggcg
     1201 gctttgcgga atgccgcacg ttgcctcttg caccgcccga aatccgtatg ccgccgccga
     1261 aaatgccgtc tgaaggcact tcccccttca gacggcattg cctgccgccg tatttgcccg
     1321 ctatccgcaa tatcggcagt ccaatatgtc tttgcggatg tcgttcagca ggaaggctgc
     1381 ggtgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgataaa
     1441 gattttttgc agggcgcagg catcggtcag ggtgtgttcc tgcaggaagc tgcggatgtc
     1501 ttcgggcaaa gtgtagtcgc gggcgacggc ggcaaagcgg gcatcggttt gcaggcggtc
     1561 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
     1621 gacaaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
     1681 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
     1741 caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
     1801 gatgttcgcc tccatcagtt gatgggcgac gggtgcaacg ggcgaaacgg aatggcagac
     1861 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
     1921 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaagggtgt attccataat
     1981 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
     2041 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
     2101 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
     2161 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
     2221 ctctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt atttcgcggc
     2281 gttgacgtaa atggtttgtc cgtcgatata ttcgtagtag gactgcattt cgcgtgcaat
     2341 cgccgcgccg gaggtttcgg gttcggtaac acccaaaccg ccgccctcgc ctttgaaaat
     2401 catctccaaa ccttgcgcga cttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
     2461 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
     2521 caggacttct tggatttcaa actggctgcc cttgcgcccg ccgtattttt tgtcgaggaa
     2581 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
     2641 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
     2701 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
     2761 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
     2821 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagacg gcatcgcgtc
     2881 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
     2941 ccccatcccc aaaccccgtg aaaaatcccg ttggcccaat ctttcgcaag gctcgctgcc
     3001 cttggctttg gcgcgttatc tgccgcataa gcagctcaag gttgtcttga ctcaagatgc
     3061 ggaacaggcg ctacgccttc agacggcatg gctgtttttc cgtccgcacg atacggcggt
     3121 gttcttgccg gactgggaaa cgctgcctta cgagcgtttt tcgccgcatc aggatttagt
     3181 gtcggagcgc ttgtcggcat tgtggcagat taaaagcggc gcggcggacg tgttgttcgt
     3241 gccggttgct acggcgatgc agaagctgcc gcctgtgccg tttttggcgg ggcgcacgtt
     3301 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaagcgatt tggtggatgc
     3361 aggttataac catgtttccc acgttgttgc ggcaggcgag ttcgctgtgc gcggcggcat
     3421 tgtcgatttg ttccccatgg gcagcgaaat gccctaccgc atcgatttgt ttgacgatga
     3481 aatcgacagc atcaaaacct ttgatacgga cacgcagcgc accatctccc ccgtttccga
     3541 aatccgcctg ctgccggcgc acgaattccc caccgacagc gaggcgcaaa aaatcttccg
     3601 cagccgcttc cgcgaggaag tcgatggtaa tccgaacgat gcggctgtgt acaaagcagt
     3661 aagcaacggc cacttcggtg cgggcgtgga atattatctg ccgctgtttt ttgaaaacga
     3721 gctggaaacg ctgtttgact atatcggcga agatgcgccg tttgtttcat tgggcgacgt
     3781 tcacgccgag gcaaaccgct tttggagcga cgttaaatca cgttacgcca tggcgcaggg
     3841 cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg atatttttgc
     3901 aggccgtctg aaaaactacg gacaagtgct gcccgatgtt tccggcaagg aacacatcct
     3961 gcccgacctt gccgtcaacc gccaatccga agagccgttg caggcattga aggattttca
     4021 gacggcattt gaaggacgga ttttgctgtg cgccgaaagt ttgggacggc gcgaaaccat
     4081 gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact ggcagggctt
     4141 cttgtcggcg catgagccgc tgatgattac agttgcgccg ttggcatacg ggttcaaact
     4201 gggcggactg caatcgccga accaacagca aactacttcc gcctccgaag gagaaggcga
     4261 tgcggttacc ga
//
LOCUS       DAWKOD010000163         4128 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000163 DAWKOD010000000
VERSION     DAWKOD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.163"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            54..581
                     /locus_tag="WJK73_001828"
     CDS             54..581
                     /locus_tag="WJK73_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ7558269.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            669..1724
                     /gene="plsX"
                     /locus_tag="WJK73_001829"
     CDS             669..1724
                     /gene="plsX"
                     /locus_tag="WJK73_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ7558270.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            1860..2111
                     /locus_tag="WJK73_001830"
     CDS             1860..2111
                     /locus_tag="WJK73_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7558271.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2361..2552
                     /locus_tag="WJK73_001831"
                     /pseudo
     CDS             2361..2552
                     /locus_tag="WJK73_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2572..2775
                     /locus_tag="WJK73_001832"
     CDS             2572..2775
                     /locus_tag="WJK73_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558272.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            2802..3764
                     /locus_tag="WJK73_001833"
     CDS             2802..3764
                     /locus_tag="WJK73_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ7558273.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLNGNEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3811..>4128
                     /locus_tag="WJK73_001834"
     CDS             3811..>4128
                     /locus_tag="WJK73_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ7558274.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         1099 a         1200 c          981 g          848 t
ORIGIN      
        1 ggtttttgtt aatccgctat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
       61 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
      121 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
      181 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
      241 acgacagcga caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctcgacc
      301 ccgttccaga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
      361 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
      421 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
      481 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
      541 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
      601 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
      661 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
      721 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
      781 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
      841 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
      901 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
      961 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaga
     1021 ccatacccgg tatcgaacgc cccgccatcg ccaaattcct cccgtccgac accgaccatg
     1081 ttacccttgc actcgacctt ggcgcgaacg tcgactgcac gcccgaacag ctcgcccaat
     1141 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgcg
     1201 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     1261 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     1321 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     1381 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     1441 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     1501 tcgacccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtgattaaaa
     1561 gccacggcgg cacagacgaa accggtttcc gctatgccct cgaagaagcc taccacgaag
     1621 ccaagtccgc cggcctttcc aaaatcgaac agggcgtagc cgaacaactc gccgcactcg
     1681 aaactgccaa agccgtccaa aacgaaaatg tcggcggtct gtaacacaca cgatgccgtc
     1741 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
     1801 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggaat atttgaaaaa
     1861 tgaagacatt agaaaaacgg atgaaagctc tagacaaacg gatgatgaag ttcggaaaat
     1921 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
     1981 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
     2041 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
     2101 cgtccgatta accaataaag ccaatgattt gataagaatg aaaacatcga cggtcgtttt
     2161 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
     2221 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
     2281 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tgtctatttt
     2341 gaaaaggata ttatttgcca atgattcaaa caattttaga agacggggat attgaagtca
     2401 aaaaccttaa aacggaaaat tacagtggga aggcaaggga aattttggga gattcctacc
     2461 ctatttagca tacctctaaa aatatttcta caattcaaaa tataatttct gaatttatga
     2521 tacaaaaaca aacaccggca ccaataattt gatggaaacc caacaatcaa aatgccgtct
     2581 gaagcatgtt tggttttcag acggcatttc cttcgcttaa aacagcgtat cggcaacccc
     2641 gccctgcctg tccacggcaa tctgcatctg aaaaccatct gtatcccaaa ccacaccccc
     2701 atcccgattt ccgtcatttg caccctgccc gtattgggca atcatcggtt tttcgcttac
     2761 aatagccgaa tctgaaccaa ctatccaaaa aggccgttcc catgcagtat gccaaaattt
     2821 ccggcacggg cagctatctt cccgccaacc gcgtcagcaa tgacgacctt gcccaaaaag
     2881 tggatacctc ggacgagtgg attaccgcgc gcacgggcat caaattccgc catattgcag
     2941 ccgaaaacga aaaaaccagc gaccttgccg ccgaagcggc gcgccgcgcg ctggatgccg
     3001 caggtttgaa cggcaatgaa atcgacctga ttatcgtggc gacggcaacg ccggatatgc
     3061 agtttccgtc tactgcgacc atcgtgcagc aaaaattggg cataaccaac ggctgccccg
     3121 cgtttgacgt acaggcggtg tgcgccggct ttatgtacgc gctgaccacg gcaaacgcct
     3181 acattaaaag tggcatggcg aaaaacgcgc tggtcatcgg cgcggaaacc ttcagccgca
     3241 tcgtcgattg gaacgaccgc acaacctgcg tattgttcgg cgacggcgcg ggcgcggtgg
     3301 ttttaagcgc gtcggacacg ccgggcatca tccacagcaa actcaaggcc gacggcaatt
     3361 atctgaaact cttaaacgtc cccgggcaaa tcgcctgcgg caaagtttcc ggttcgccgt
     3421 acatttcgat ggacggtccc ggcgtgttca agtttgccgt caaaatgctg tccaaaatcg
     3481 ccgatgacgt tatcgaagaa gccggttata ccgccgctca aatcgactgg atcgtgccgc
     3541 atcaggcaaa ccgccgcatt atcgaatcga ccgcgaaaca tttaggtttg agtatggaca
     3601 aagtcgtcct gaccgtccaa gaccacggca acacatccgc cgcctcgatt ccgctggctt
     3661 tggatacggg catccgcagc ggacaaatca aacgcggtca aaacctgctg ctcgaaggca
     3721 tcggcggcgg tttcgcgtgg ggcgcggtgc tgttgcaata ttgaacccga tgccgtctga
     3781 aacaggcttt cagacggcat ttcccatatc atgaagcggc aggctttctt caaactgatg
     3841 gcgtgtgcgg catttctgtc tgccgtttcg ctgcgcctcc ccgtattggg cgcgtgttac
     3901 gcaatattgt ccctctatgc gtttgcactt tacggcatcg acaaacggcg tgccgtgcgg
     3961 ggaaaacgcc gcattcccga acaccgcctg ctgctgcctg ccttgttcgg cggttgggcg
     4021 ggcgcatact tgggcagcag gatattcagg cataaaacgg cgaaaaagcg ttttgttgtg
     4081 ctgttccgtc tgactgtttc gggcaatgtc ctggcgaccc tcatcctg
//
LOCUS       DAWKOD010000164         4095 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000164 DAWKOD010000000
VERSION     DAWKOD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.164"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(202..1098)
                     /gene="argB"
                     /locus_tag="WJK73_001835"
     CDS             complement(202..1098)
                     /gene="argB"
                     /locus_tag="WJK73_001835"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ7558275.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRMPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1268..2398
                     /locus_tag="WJK73_001836"
     CDS             1268..2398
                     /locus_tag="WJK73_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ7558276.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2469..3320
                     /locus_tag="WJK73_001837"
     CDS             2469..3320
                     /locus_tag="WJK73_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ7558277.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWSGWLQQYSMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3403..4065)
                     /locus_tag="WJK73_001838"
     CDS             complement(3403..4065)
                     /locus_tag="WJK73_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ7558278.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          908 a         1193 c          989 g         1005 t
ORIGIN      
        1 aaaatcaaag ccggagaatt tatcccaaac aaccggattt caaaaaaacc agattcccgc
       61 cttcgcggga atgacggatt ttaggtttct gttttggttt ctgttttcgc gggaataaca
      121 acttagaaat tgcccgaaac cccaaaaaca gaaaccggac aaacgggttt tccgccaaag
      181 ccggcatttt ccgactttgc ttcaggcatc ttccccaccg cccaaaatca tcgaaccgat
      241 accggcatcg gtaaagattt ccagcaaaag cgcgttgggc atcctgccgt cgatgatgtg
      301 cgtggctttc acgccgttga cggcggcttc gaccgcggaa gcgattttcg gcagcatacc
      361 gccatacagc gtgccgtcgg caatcagttc atcaatccgt ttcggcgtga gtttggtcag
      421 cagattgccc gttttgtcca tcacaccggc gatattcgtc atcatcaaga gtttttcagc
      481 gttcaattct tccgccaatt tgcctgctac caaatcggcg ttgatgttga acgcttcgcc
      541 tttttcacct acgccgacgg gggcgacgac gggaatgcag ccacgttcta tcagcccttt
      601 aaccaaaccg gtatcgatgc tttccaccgt accgacctgt ccgatgtcca cgccattctg
      661 ttcgggcgta tcgatcaaaa gtttcttcgc cttaatgaaa tggtcgtcgc gtccgcttac
      721 gccgaccgcg tgtccgccat atgtgttaat catcgacacg atttctttat tgacatgccc
      781 gcccaacacc atttcgacaa tatccatcgc ctctttgtcg gtaacgcgca ttccttggac
      841 aaactcaccc tttttgccga ctttttcaag catcgcattg atctgcggcc cgccgccgtg
      901 aacgatgacg ggatgaatgc cgaccagctt cagcagcacg acatcgcggg caaacccttc
      961 tttcaaggca ggttcggtca tcgcgttgcc gccgtatttg atgacggcga ccgaaccgga
     1021 aaaccggcgg atgtaaggca gcgcttcggc aaggatacgc gccttgtcgg cggcggaaat
     1081 aatgttttca gactccataa cggctcccta ttgttgacaa tttcggctta tgttaacgcc
     1141 aaacgccgtc tgaaacaatc ttcagacggc atccggcaga ctttcgggca ggttgaagcc
     1201 ttgcgatacg ggcggtaaaa tcgcggttta tccggttcga gaaaccggcg acagcaagga
     1261 ctttcccatg attaaaatca gcacccgctt cgatgccggc tcggtcgtcg tcaaagacct
     1321 gaccgatcct tccaacatcc gcctcgccct gcgtcccgac aacgcctccg attttgcaca
     1381 atggttctac ttccggctgc aaggcgcggc atatcaaaac tgcatcatgc attttgacaa
     1441 tgcggcagaa gccgcctacc cgaaaggctg ggaaggctat caggcgtgtg cctcatacga
     1501 ccgccgcaac tggttccgcg taccgacttc ctacgaaaac ggcgtactga ccgtcaatca
     1561 tacgccgctg tccaacagcg tgtattacgc ctatttcgaa ccttactccg aagaacagca
     1621 tttaaacctc ctcggcgacg cgcaaggcag cggcctgtgc cgcatcgacg acttgggcag
     1681 caccgtgcaa gggcgcgaca tcaatctgct gaccataggc aatcaggtcg aaagcgattt
     1741 gaaaatctgg attaccgccc gccagcatcc aggcgaaacc atggcggaat ggtttgtcga
     1801 aggactgctc ggcaggctgc tcgaccatca ggatgcaacc gcgcgcaccc tgctcgatcg
     1861 cgccacgttc tatatcgtcc ccaacatgaa ccccgacggt tcggcactgg gcaaccttcg
     1921 caccaacgcc gccggtgcga acctcaaccg cgaatgggaa aatccgactt tggaaaaaag
     1981 ccccgaagtg ttcttcgtgc gcgaaaaaat gctggaaacc ggcgtggatt tgttcttgga
     2041 catccacggc gacgaagggc tgccctttat ctttgttgca ggaactgaag gcgtgccaaa
     2101 ctacaatccg cgcatttccg cgttggaaac acagttcaaa accgcccttt tgaacgccag
     2161 ccccgatttc caagacgaat acggttacga aaaagatgcg cccggtcagg caaacatgac
     2221 cttggcaaca aactgggtcg gcaaccgttt caactgcctt gcctatacgt tggaaatgcc
     2281 ctttaaagac aatgccaacc tgcccgacga cgatttcgga tggaacggtc aacggtcgtt
     2341 gcgcttgggc gaagccgcgt tgtccgccat cctcaacatc atcggcgatt tgcgctgaaa
     2401 cccttattgc agaaatgccg tctgaagcat catgcctttc agacggcatt tgaaaacgaa
     2461 agcctgttat gattatccat ccccaattcg atcccgtcct catcagtatc ggcccgcttg
     2521 ccgtccgctg gtatgcccta agctacatcc tcggatttat tctttttacc tttctcggca
     2581 gaaggcgcat cgcgcaaggc ttgtccgttt ttaccaaaga atcgcttgac gacttcctca
     2641 cttggggcat tttgggcgta atttttggtg ggcgtttggg ttacgtcctg ttttacaagt
     2701 tttccgacta cctcgcccat ccgcttgata ttttcaaggt atgggaaggc ggaatgtcgt
     2761 tccacggcgg ctttttgggt gtagttattg ccatatggtt gttcggccgc aaacacggca
     2821 tcggcttcct caaactgatg gacacggtcg caccgctcgt tccgctgggt ctcgcttcgg
     2881 gacgtatcgg caacttcatc aacggcgaac tttggggacg cgttaccgac atcaacgcat
     2941 tttgggcaat gggcttcccg caggcgcgtt acgaagatgc cgaagccgcc gcgcacaatc
     3001 ccctttggtc aggttggttg caacaatata gtatgctgcc ccgccatccc tcgcagcttt
     3061 atcagtttgc acttgaaggc atctgcctgt tcgccgtcgt ttggctgttc tctaaaaaac
     3121 agcggccgac cggacaagtc gcctcgctct tcctcggcgg ctacggcata ttccgcttca
     3181 ttgccgaatt cgcacgccaa cccgacgact atctcgggct gctgaccttg gggctgtcga
     3241 tggggcaatg gttgagcgtc ccgatgattg ttttgggtat cgtcggcttt gtccggttcg
     3301 gcatgaaaaa acagcactga ccgcctgtga aaaataccgt ctgaaacatg gaaatccggt
     3361 ttcagacggc atttttacgt ttggttcatg gaaacaaacg cttcaattct taccgcaaca
     3421 cgccttgtat tttctgccgc tgccgcacgg acagggatcg ttcctgcctg ttttttcgcc
     3481 ttccctgcgg acggtttgcg gtttgttgat gactgcctgc cagtagcggt agatatccgc
     3541 caatgcataa ggcaactcgg attccagttc cgccagctcg ccttctgtga attgcagacg
     3601 gatagtgccg ttttcctctt cgtcgtaaat accccccaat gccatgatgg gataaaacaa
     3661 ctcttcaaac gcttcatcat cgacggcttc aaaccaatcg gtcggcacaa tatccaaacc
     3721 gtaaagataa gcattgcacc atgtgtaaaa atcgctgccg ccgtcttcgt tttcatacag
     3781 ccacaaatcg ggcagttttt tatccgacat cgcggcggtt gtttccatcg ccattgccaa
     3841 aaccagccgt tcgatttcgg aacgttcggc ggcggtaaat tgcgattcgt cgcccaacac
     3901 ttcgggcagc cagtcgagcg gtgtcaattt gtccggcccg ctcaacagcg ccgtcataaa
     3961 accttgaacc tcgtcgcaac gcatcgtgtt gccttgttcg cttttggcat ccaacaattc
     4021 gctcaaccgc cgtttggatg cttcggtaaa ttttcgggaa tccatcattt tccttttcaa
     4081 atgggttttg cgccc
//
LOCUS       DAWKOD010000165         3996 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000165 DAWKOD010000000
VERSION     DAWKOD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.165"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(67..1317)
                     /locus_tag="WJK73_001839"
     CDS             complement(67..1317)
                     /locus_tag="WJK73_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ7558279.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1384..1579)
                     /locus_tag="WJK73_001840"
                     /pseudo
     CDS             complement(1384..1579)
                     /locus_tag="WJK73_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WJK73_001841"
     CDS             complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WJK73_001841"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ7558280.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3741..3975)
                     /locus_tag="WJK73_001842"
                     /pseudo
     CDS             complement(3741..3975)
                     /locus_tag="WJK73_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
BASE COUNT         1002 a          956 c          937 g         1101 t
ORIGIN      
        1 tccgcctgaa acattatgag atttcaggcg gcattggatt gcttagcgga atatttttaa
       61 aaaggcttac gcgccgtaaa cggggtattt attgcacaaa gcagttactt gttcgcggac
      121 tttggcgagg ttggcttcgt cttcggggtt agacaatacg tcggcaacca agttcgccaa
      181 tacgcgcgcg tcggcttcgt taaaaccgcg tgtggtcatg gcggcggaac cgatgcggat
      241 gccggaggta acgaagggtt tttccggatc attcgggatg gcgtttttgt tgacagtgat
      301 gtgcgctttg cccaaagcag cttcggcggc tttgccggta attttcatcg gttgcaggtc
      361 aacgaggaaa acgtggcttt cggtgcggcc ggaaacgatg cgcaaaccgc gtttaaccaa
      421 ctcttccgcc atggcggctg cattgatttt cacttgtttt gcgtattgtt tgaactcggg
      481 ttgcaatgct tctttaaacg ccacggcttt ggcggcgata acgtgcatca gcgggccacc
      541 ttgcaggctt gggaagatgg aagagttcaa cgctttttcg tgggtattgt cgcggcacaa
      601 aattacgccg ccgcgaggac cgcgcagggt tttgtgggtg gtggtggtaa cgaagtcgca
      661 gaacggcacg ggattgggat attcgccgcc ggcaaccaga ccggcatagt gcgccatatc
      721 gacaaagagg tatgcgccga ctttatcggc gatttcgcgg aattttgccc agtcgatttg
      781 caacgcgtag gcagacgcac ccgccacaat cattttgggt ttgtgttcga gcgcgaggcg
      841 ttcgacttcg gcataatcga gcacttcgtt ttcatccaaa ccataagtaa cagcgttgta
      901 gagtttgcct gagatattaa cgctcgcgcc gtgggtcagg tggccgccgt gcgccagaga
      961 catacccaaa atggtgtcgc ctggttttaa aacggaagcg tacacggctt ggttggcttg
     1021 cgagccggag tgcggttgga cgttggcgta ttgcgcgccg aacagttttt tgacgcggtc
     1081 aatcgccaat tgttcgacaa tatcgacgta ttcgcagccg ccgtagtagc gtttgccggg
     1141 gtagccttcg gcgtatttgt tggtcagctg ggaaccttgc gcttccatta cggcgcagct
     1201 gacgtagttt tcagaggcaa tcagctcgac gtggtcttgc tggcgttggt cttcttgggc
     1261 aatggctgct gccaaatcgg ggtcatattg tgcgagggta acgctttttg aaaacatgtt
     1321 ctcggctcct ttgtgtaatc agggtatcat gagtgttttt tgtataaaaa aatacttcaa
     1381 aacctaaggc agatagtcca taatgcgtaa attttctttg gcattatcag gtaatttatt
     1441 taacatgctg gtttttagcg tctcattacc tttatgttag tacaaggcta cccaaaggca
     1501 atgcatcaaa tggtaaattt tcggcagttt gtgcaaaatc aatcaaataa ccagcaccaa
     1561 ccacatcttt atcagccata acccgctgat acaccacttg ccaagacaac tctcctgccc
     1621 caccatgtct taaaatcaga tgaatggctt gtaatgctgt gtttggcgtc tcacaaattg
     1681 cgaagatctg ctcagaagct tgcttgatgg cttgtatttt tgtttgatta ctcacaattt
     1741 ccccctattt taatattaac ttaatgcggt caaattcaca aaatacaagc ttcacctcta
     1801 atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc tgcttggctc
     1861 gataataatg gcttgaacac ctgaatttag ctcacgcacg tcagtcttat agcccaaatc
     1921 atttaatgct tgttgccact ggagggcggt tgtacccgtt tctagttcat agctaccaaa
     1981 gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc agtcactatg
     2041 tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg agccgattgc
     2101 catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc gtggtctctt
     2161 gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa aactaaagtc
     2221 tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa acgcattttc
     2281 aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata tatgactggt
     2341 agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc ctgcagatac
     2401 gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta ggtagtcttt
     2461 agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat atacatcacg
     2521 atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag catcataccc
     2581 cacctgattg ggtgaaaatt catttaaaat ccccaaaatc tgacccacag caatccctcc
     2641 tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac aaacaggcgg
     2701 gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg ggttatcctt
     2761 agcattttgg acaactgaaa cgatattttg ggcatattta ccagtatgca gagcttttgc
     2821 accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca gcaagctgcc
     2881 tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat agcgtgccaa
     2941 atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa agccttgttt
     3001 tgccaagcgg atcggtgtat caaataattt tccccaaggc aatacaccgt atcgctgatg
     3061 tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac cgaccaccgc
     3121 ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg tcgcacgcat
     3181 cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat cccaatacaa
     3241 cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta gtgtcgtctg
     3301 caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat agccagcttg
     3361 tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct gcttttgttc
     3421 ggtcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtgatga cagcagaatg
     3481 agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg tcatagacat
     3541 tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg agtttgattg
     3601 atgattcata caaggcatgc tgattattgt atttaatatg actaaataat tcaatccaaa
     3661 ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt ttttgatatt
     3721 tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag tggttttagc
     3781 tcacctgatt ggtacatttg tgataatatc actaccaccg attaactcac cattaaccca
     3841 aagctgtgga aaggttggcc gattggcgat gagtggtaga gtactgcgaa tttctgggtt
     3901 ttctaggata ttaacaaaag caaagggtct gccaattggg tcagcacctc tactgcacgc
     3961 gctgaaaatc cacattgggg aaactggggc gtgcct
//
LOCUS       DAWKOD010000166         3983 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000166 DAWKOD010000000
VERSION     DAWKOD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.166"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            101..1243
                     /locus_tag="WJK73_001843"
     CDS             101..1243
                     /locus_tag="WJK73_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ7558281.1"
                     /translation="MQKWQTELYSTPLWLCLTPKSTVKVLLLITVMVTLLLTEVRLNV
                     LSTFMSKGLYDSMQDLNASAFWMFAAMNAGVVLIRAFNNVVNDFLDQGLAIKWSEQLN
                     EVLTTRWLADKNYYRLQMRRHAPDNIDQRIQQDAQDFIASTIEFVRGMVNSVVTSLEF
                     AVVLWGLAGILTVFGFDIPRGIVWLVFIFVILATFIAMWIGNPLIRYNYENEKLNGDY
                     RYSLIRVRDHAESVAFYNGEQHEHGRLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNG
                     IKLFPIILQAPRLFAGQIKIGDIQQTVQAFARLQNALSFFRMFYNKFTAYPARLERLY
                     GFLLSTEEQHSARQPEISEVSDGIALECKAAAAFARIGGSFCTAEA"
     gene            complement(1353..1562)
                     /locus_tag="WJK73_001844"
     CDS             complement(1353..1562)
                     /locus_tag="WJK73_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558282.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1559..1855)
                     /locus_tag="WJK73_001845"
     CDS             complement(1559..1855)
                     /locus_tag="WJK73_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ7558283.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1834..2439)
                     /locus_tag="WJK73_001846"
     CDS             complement(1834..2439)
                     /locus_tag="WJK73_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558284.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKIGKAAAAARFAEQKTATAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            2519..2806
                     /locus_tag="WJK73_001847"
     CDS             2519..2806
                     /locus_tag="WJK73_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ7558285.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2778..3080
                     /locus_tag="WJK73_001848"
     CDS             2778..3080
                     /locus_tag="WJK73_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ7558286.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT          806 a         1076 c         1095 g         1006 t
ORIGIN      
        1 cacgcaatgt accctgaccg gcaaaacttc cccaaacttc atcaaaaaat caactgtacc
       61 cgtctttttc agacggcatt gataccgtaa gaaatttccc atgcagaaat ggcaaaccga
      121 gctttattcc acaccgcttt ggctttgcct gacaccgaaa agcacggtca aagtcttgct
      181 gctgattacg gtaatggtta cgctgctgtt gaccgaagta cggctgaatg tgttgagtac
      241 ctttatgtcc aaaggacttt acgactcgat gcaggatttg aacgcctccg cgttttggat
      301 gtttgccgcg atgaacgcgg gcgtggtgct gatacgggcg tttaacaatg tcgtcaacga
      361 cttcctcgat caaggcttgg cgattaaatg gtcggagcag ctcaatgaag tgctgacgac
      421 gcgctggctt gccgacaaaa actactaccg cctgcaaatg cgccgccacg cgccggacaa
      481 catcgaccaa cgtatccaac aggacgcgca ggatttcatc gcttcgacca tcgaatttgt
      541 acgcggcatg gtcaattcgg tcgttacctc tttggaattt gccgttgttt tgtggggctt
      601 ggctggcatc ctgaccgtgt tcggcttcga cattccgcgc ggcattgttt ggctcgtctt
      661 tatttttgtg attttggcga cctttatcgc gatgtggata ggcaacccct tgattcgtta
      721 caactatgaa aacgaaaaac tcaacggcga ctaccgttat tccctcatcc gcgtacgcga
      781 ccacgccgaa agcgtagcgt tttacaacgg cgaacaacac gaacacggcc ggctttccga
      841 ccgctttaaa gccattatcc gcaaccgttg gcgcatcgcg cggcaaagcg tctgcttaag
      901 cggctttaac gatatgttca ccaacggcat caaactcttc cccattattt tgcaagcgcc
      961 gcgcctgttt gccggacaaa tcaaaatcgg cgacatccag cagaccgtcc aagcctttgc
     1021 gcgactgcaa aacgcgctat cttttttccg gatgttctac aacaaattca ccgcctaccc
     1081 cgcccgattg gagcgtctgt acggcttttt gctgagtacc gaagaacaac acagcgcgcg
     1141 gcaacccgag attagcgaag tttcagacgg cattgcactg gaatgtaagg cggcggcagc
     1201 ttttgcacga attggcggca gcttttgcac cgccgaagcg taggaaattg tattagcttt
     1261 tgcacaaatt tatccccgtt taatggtctg ttaaacgggg attatcttat gcctttaaaa
     1321 gtagtacgct acttgcggtc agaacttata aatcaggtgg tcgtcgtagc ttgaattgct
     1381 gttgatgtgc tgctcacagt caaggtttgg ctgcctgcaa aacgctccca tccggtcagg
     1441 cgcagttctg ccacgaggtc aagataagta ggcagttcgc tgcgggtgcc ggagaagaag
     1501 acaaatggcg gccgcaccat tgccatcagg cgcagaaact ctaccatgcc gaagtagttc
     1561 atcgcgtccc cttttccccg gcggcgcggc tttggttgaa ccgcgcgtcg tgctgcctgc
     1621 ctttcagttc gctgatatac gcctgccggc agtggtggcg gtcgaaaaat acgccgttga
     1681 tcagggtata taaaaccttc cagcgggttt tgggcttttg ggcgagccgc gcgccgcgat
     1741 aggtgcggct tgagagggtt tcgtccgccg cgccgccggt tagggcgttg aacagttggt
     1801 cgatggctac caacaggtgg taaacgtatt ttttcagccg atgtttagcg tccattcttc
     1861 gatttccttt tccagcgcgt ccaatcctgg cgcggtttcg atggcgttca atttgtcttc
     1921 gagctgctga cgctttccga taatcgcgcc ggcggcaaca gccaggcggg cggatttttc
     1981 gacaactttt tcaatcaaaa cgtccaattc cacgcccctt gcggcggcga tttgcgccag
     2041 catcggggtc ggggcgttgt tgtccgcctg ccgggcgagg gcttcttttt cctgcctgta
     2101 aaagctgtcg atttccactt ggggatagcc cgccaagagg ctgtttttga gttcgtccgc
     2161 cttttccgcg aggcggaatg ccgtggcggt tttttgttcg gcgaaacggg cggctgcggc
     2221 ggctttgccg attttccatt ttttgccgtc ccattcgtgg taatcggacg ggcgcggcgg
     2281 ggttaaaacg gggcggccgt cggaatcggc ggcaatctgc ccgccctgcg cctgtcctgc
     2341 caaaagggcg gcgtattctt cggcgcggac ggcaaccgcg ccttcgggga tgctgcccaa
     2401 ggtgtcgtcg taaaagccgt ttttgaaata gatggtcatt tcggggtttc cttttttgta
     2461 tttacatttt ggttttttgc cgcttataat gtaacgacaa aatacgctgc aagctgcaat
     2521 gaagattgaa tttgactccg aaaaaaatca acgcaatatc gaagagcgga atttgccctt
     2581 tgaatcagtc gggcaaatac gctggacaac ggccgttatt gtgcccgatg tctgctttga
     2641 ttaccccgaa ccgcgttatg ttgccgctgc ttatctaggc gatacgcaac gcctgcacat
     2701 tgtctgtttt acgccgataa aagacggcat ccgtgtcatc agttttcgca aagcgaataa
     2761 aagagaggtt aaaaaatatg caacgtcaat ccttaacaaa cgctgacggc gaagtgcggg
     2821 aacttgccgc cgaagatttt gcgcttgccc gccccattgc cgaagccctg cccgaagact
     2881 tggcacaggt gcttttttcc catcaaaaac aattggaaga aaagggcgta atgcaaaaac
     2941 gaaacacagg caaaaccccg aaacaactcg ttaccatacg cctgtccgcc gatgtggtcg
     3001 aaaaattccg cgccggcggc aagggctggc agacgcgcat caacgaggtt ttgcgccaat
     3061 atgtcgccca attgaaataa cgggggcggc cgctgcttat cgggcgcgcc cgactatcct
     3121 ttcccgcgcg gtttcggccg tgcggttttt tgttgccggt gccgtccgaa gggggcagcg
     3181 gctttttgcg cggtcattcc cgcctttcgt tattcccgcc actttccgtc attcccgcga
     3241 aggcgggaat cccgaaaccc ctgccgccgt cattcccgcg caggcgggaa tccagtccgt
     3301 tcggtttcag ccgtttccga tagattcctg ccgcgtttgg ggtctggatt cccgcctgcg
     3361 cgggaatgac ggcggcgggg ggtcggtttt ttccgataga ttcctgccgc gtttggggtc
     3421 tggattcccg cctgcgcggg aatgacggcg gcgggggttt ctgttttttc cgataaattc
     3481 ctgccgcgtt tggggtctgg attcccgcct gcgcgggaat gacggcggcg ggggtttctg
     3541 ttttttccga tagattcctg ttgcgtcggg gtctggattc ccgctttcgc gggaatgacg
     3601 gcggcggggg tttctgtttt ttccgataga ttcctgccgc gtttggggtc tagattcccg
     3661 ctttcgcggg aatgacggtg gcgggggttt ctgttttttc cgatagattc ctgccgcgtt
     3721 taggggctgg attcccgctt tcgcgggaat gacgaaagcg gcgggaatga cgaaagcggc
     3781 gggaatgacg aagagtggca ggattggcgg ggcataggtt tcgggggatt ggcggcgcgg
     3841 ccggtttcgg tcggattgct tggatcggcg ggctgaagcc catctctaca actcgtctct
     3901 acaacccatc tctacaaccc atctctacaa cccatctcta caacccatct ctacaaccca
     3961 tctctacaac ccatctctac agc
//
LOCUS       DAWKOD010000167         3931 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000167 DAWKOD010000000
VERSION     DAWKOD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.167"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..332)
                     /locus_tag="WJK73_001849"
     CDS             complement(<1..332)
                     /locus_tag="WJK73_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7558287.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAAHPDAETPPPENSVKEALKRVGFGKVSFDDREIRFTEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WJK73_001850"
     CDS             complement(313..594)
                     /locus_tag="WJK73_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ7558288.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WJK73_001851"
     CDS             complement(603..1397)
                     /locus_tag="WJK73_001851"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ7558289.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WJK73_001852"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WJK73_001852"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ7558290.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3257..3631
                     /locus_tag="WJK73_001853"
     CDS             3257..3631
                     /locus_tag="WJK73_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558291.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3675..>3931
                     /locus_tag="WJK73_001854"
     CDS             3675..>3931
                     /locus_tag="WJK73_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ7558292.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1066 a         1017 c          927 g          921 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggtaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgcttcaggg cttccttcac gctattctcc
      121 ggcggcggtg tttccgcatc aggatgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcctgtct gtcgtccaca ccgaacaggc gcagctccgc
      421 gccggaaccc agcgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcttt
      481 attgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaactg tcaaaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatgtgc ggatgcggat cgtagccttc caactcaaaa
      661 tcttcaaatt tgaaggcaaa caaatctttg acttcaggat tgattttcat cacaggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggat aaactcaccg gcttccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcgatgttg aacggtacgc caaggaaaat atccgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 caaggcggca aggccatctc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg ggtttttctt aatttgttcc accacattgg cgatttggtc gatatggcgg
     1081 ccgtcgggcg cgggccagtt gcgccactgg tagccgtaaa ccgggcccaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct tcaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtgcccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacaggca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaacatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 ttttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtcatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgat gttccggccg gcgacatcgg
     2281 cgtaggcggg cgcgaaatcg gctacctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggttc cggcaacgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggcgcga aagtgctgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaccaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtagcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 attatccgcc cgttcgaatc gggtgatact atttgtatcc agaattgttg gatagtttgg
     3241 gagtggagag gaagaaatga ttatggacca atactatatg gagttaaaga ataagttatc
     3301 taacagacca atcctactgg ataatactaa tgattttctt tttgttttag taaatacagt
     3361 gaaagctatg atagaaaata ctgataaaag tcagctttca gagttagata aaattttaga
     3421 cggtgtcaca agtcaagaat tgaaacttgc ctatgatttt tgtcaaggga aattcggtca
     3481 agcaggtttt tcttatcgta ggcacccgaa ttatttttat ttgtccagtt taattgcgac
     3541 ttttccagaa tttgagttgt ctaaagcaga ccgagattac ctcaaaggaa tcataaattt
     3601 tgataactac cttttatatg agttagatta gtctttaatc acctcaaaac cctatttatt
     3661 caaaaggaga aaaaatgatt gagctgaaat tagtagatga gagcagtttt caggcagtgc
     3721 tggatttgaa aatatctgaa gctgatgaac gagcacgttt tgtggctcca aatgtacgct
     3781 ctttagctga cgcatggctc taccgggaaa atgaagatgt gtttccgagg gcaatctatt
     3841 gggataagca agtggttggc tttctcctgc tggaaataga caaggatgaa gcagaatact
     3901 ttatctggcg gataatgatt ggtcagcagt g
//
LOCUS       DAWKOD010000168         3908 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000168 DAWKOD010000000
VERSION     DAWKOD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.168"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            150..1667
                     /locus_tag="WJK73_001855"
     CDS             150..1667
                     /locus_tag="WJK73_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7558293.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNQNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1728..3509)
                     /locus_tag="WJK73_001856"
     CDS             complement(1728..3509)
                     /locus_tag="WJK73_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ7558294.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDRALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPSGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQIGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          815 a          876 c         1284 g          933 t
ORIGIN      
        1 atgtgaaatc cgccctttga aaatcgggcg gcggtttttt gcctgcccgc tttcagacgg
       61 caaagccggt tttcacgggt ttctgcctgt ttttcggatg gtttgacgtg cttcggcggc
      121 gtgtttgccg gaaaggtaaa tgacagggta tgttgtattt cagatacggc tttttggttg
      181 tttggtgtgc ggcaggtgtt tctgccgcct atggggcgga tgcgcccgcg attttggatg
      241 acaaggcatt gttgcaggtg cagcggtcgg tgtcggataa gtgggcggaa tcggattgga
      301 aagttgaaaa tgatgccccg cgcgtggttg acggggattt tttgttggcg catccgaaaa
      361 tgttggaaca tagtttgcgc gacgcgctca acggcaatca ggcggattta atcgcttcgt
      421 tggcggattt gtatgccaag ctgccggatt atgacgcggt tttgtacggc agggcgcggg
      481 ctttgctggc gaaattggcg ggaaggccgg cggaggcggt ggcgcggtat cgggaactgc
      541 acggggaaaa tgcggcagac gagcggattt tgctggattt ggcggcggcg gagtttgacg
      601 atttccgact gaagtcggca gaaaggcatt ttgcggaggc ggcaaaattg gatttgccgg
      661 caccggtttt ggaaaatgtg gggcgttttc ggaaaaaaac ggaggggctg acgggctggc
      721 gtttttcggg cggcatcagt ccggcggtca atcaaaacgc caataatgcc gcgccgcagt
      781 attgccggca aaacggaggc cggcagatat gcagtgtcag ccgggcggag cgggcggcag
      841 ggttgaatta tgaaatcgag gcggaaaaac tgacggcgtt ggcagataat cattatttgt
      901 tgttccgttc caatatcggc ggcacgagct attatttcag taaaaaatca gcttatgacg
      961 acgggttcgg cagggcgtat ttgggttggc agtataaaaa tgcacggcag acggcgggga
     1021 ttttgccgtt ttatcaggtg cagttgtcgg gcagcgacgg ctttgatgcg aaaacaaaac
     1081 gggtaaacaa ccgccgcctg ccgccgtata tgctggcgca cggagtcggc gtgcagctgt
     1141 cccatactta ccgaccaaac ccgggatggc aattttcggt cgcgctggaa cattaccgcc
     1201 aacgctaccg cgaacaggat agggcggaat acaataacgg caggcaggac gggttttatg
     1261 tttcgtcggc aaaacgtttg ggcgaatcgg caactgtgtt cggcggctgg cagtttgtgc
     1321 ggtttgtgcc gaaacgcgaa acggtgggcg gcgcggtcaa taatgccgcc taccggcgca
     1381 acggtgttta tgccggttgg gcgcaggagt ggcggcagtt gggcggtttg aacagtcggg
     1441 tttccgcgtc ttatgcccgc cgcaactata agggcgttgc ggctttctcg acagaggcgc
     1501 aacgcaaccg cgaatggaat gtctcgctgg ctttgagcca cgacaagttg tcgtataaag
     1561 gtatcgtgcc cgcgttgaat tatcgtttcg gcaggacgga aagtaatgtg ccgtatgcga
     1621 aacgccgcaa cagcgaggtg tttgtgtcgg cggattggcg gttttgaatg gtggaataat
     1681 gccgtccgaa ctttgtggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg
     1741 gcggcaaata cgccgcgcaa ggcgttggag aggcggattt cttcggcttc ttgcagtgtt
     1801 ttttgtgtga tgtgtgtttc gattacttga tttgtttgca aatatttttg cggttcgtcc
     1861 aatacggctt ggcgcattat gccgtttaaa atgtccaaat ctaaagaggg cgtaagccat
     1921 tgtccgcgat gtttgacgaa cacgttgctt ctgccgcctt cgagcaggat gccgtctgaa
     1981 ttgaaaaaca ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag
     2041 acggtgcggc aggtggtttt gaagcggcgc aggtagtttt gtgcgggcag gatggtcggc
     2101 gaaatgatga cgcgttgttt gtcggtcaga cggtttaaaa cggcgcggga caggctgatg
     2161 ccgtctgaag cgagcagggc tttgacgcgg aacgcgccat cgggcaagtc ggcaatgtat
     2221 tgtttgattt gattttcgca gccgtcgggc aggggcaggt tgagggcttg ggcggaggtt
     2281 ttcagacggc ataggtggcg gtcgagcagg gtgcagcgtc cgttttccgc gcgcagggtt
     2341 tcaaaaatgc cgaagtcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcg
     2401 cgatattcgg cggcggggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct
     2461 tgataaatgc cgtctgaaag cggcgtgagc gataaggtgc ggatgacgac gttgaacgcg
     2521 ccttcaaacc ccaagccgcc cgcgcaaggt ttcaggtagc cgatgctgcc cgtataaagc
     2581 ccacgcgctt cggtttcgag cgattcgata atctgcatac tcattttttt gggcgcgccg
     2641 gtgatgctgc cgcaggggaa ggcggcgcgg aggatgtcgg cgaacgaggt gtttggcagg
     2701 gcttgggctt ggatggtgct ggtcatctgc caaacgctgc cgaaacgcga cactttaaac
     2761 ggctcgggta cgcatacttt gccgatttgg gcgattttgc cgaggtcgtt gcgcagcaaa
     2821 tcgacaatca tcacgttttc ggcgcggttt ttcgggtcgg cttgcaattc ggcggcgcgg
     2881 cgttcgtctt gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg
     2941 gtgccgtctg aagcgatgtt gaggaagagt tcgggcgaga aacacagcgt ccacgcggat
     3001 tgcccctccg catcgggcag gtgggacaag acggcatagg ggacgggctg gcgcaggcgg
     3061 cggtagaggc tgacgggatt gccgtaggct tgcaggtgca ggcgagtggt gtagttgatt
     3121 tgataggtgt cgccgcgccg gatggcttcg tggatttggc ggatgcggtc gaggtaatcg
     3181 gtttcggata cggaggattg cggcgtggaa atgccggagg gaaggtcgtc tgagtgtcgg
     3241 gcaagccagc ttgcggcatc ggtgtcggcg cagtcggcaa accagtgcag ggcaaggttg
     3301 ccgccgcgct cggactcaac ccccgtcagc ggcaaaccga attcgtagtc tgcaaacaac
     3361 accgaatgca accctttttg ccagcccttt tgcagcgcgc ggtccaaagc atcgagttct
     3421 tcgggaccga aaaaacggct ttctacatga ttttgatagc gttttgcgcg gccgcttacg
     3481 gcatcgtcaa acagggcgaa atagggcatg gcaattcggg gcaaatgttt tgattatacg
     3541 ctctttttac acatattttc agacgattcg gcaaatatcg gcaaaatgta attttatgta
     3601 gagaaagtgg ggggcgaggg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa
     3661 tacaataagg agacctttat gacagaccac cagctgcaac cgtttgaaaa cgtagaatta
     3721 ggcgaaaagc aagaccagct ccaagtatag tggattaaca aaaaccagta cagcgttgcc
     3781 tcgctttagc tcaaagagaa cgattctctc aggtgctgaa gcaccaagtg aatcggttcc
     3841 gtactatctg tactgtcttc ggcttcgttg tcttgtcctg atttttgtta atccgctata
     3901 aagaccgt
//
LOCUS       DAWKOD010000169         3859 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000169 DAWKOD010000000
VERSION     DAWKOD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.169"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WJK73_001857"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WJK73_001857"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ7558295.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WJK73_001858"
     CDS             1439..1924
                     /locus_tag="WJK73_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ7558296.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJK73_001859"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJK73_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ7558297.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          781 a          940 c         1080 g         1058 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ttgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgttgg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWKOD010000170         3836 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000170 DAWKOD010000000
VERSION     DAWKOD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.170"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..414)
                     /locus_tag="WJK73_001860"
     CDS             complement(<1..414)
                     /locus_tag="WJK73_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ7558298.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            562..1014
                     /gene="dut"
                     /locus_tag="WJK73_001861"
     CDS             562..1014
                     /gene="dut"
                     /locus_tag="WJK73_001861"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ7558299.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1090..2277
                     /gene="dapC"
                     /locus_tag="WJK73_001862"
     CDS             1090..2277
                     /gene="dapC"
                     /locus_tag="WJK73_001862"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ7558300.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2539..3318
                     /gene="yaaA"
                     /locus_tag="WJK73_001863"
     CDS             2539..3318
                     /gene="yaaA"
                     /locus_tag="WJK73_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ7558301.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3401..3491
                     /locus_tag="WJK73_001864"
     tRNA            3401..3491
                     /locus_tag="WJK73_001864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3435..3437,aa:Ser,seq:tga)
     gene            3704..3793
                     /locus_tag="WJK73_001865"
     tRNA            3704..3793
                     /locus_tag="WJK73_001865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3738..3740,aa:Ser,seq:cga)
BASE COUNT          990 a          900 c          957 g          989 t
ORIGIN      
        1 aggcatattg aatgcgggca aaccggctgc tttccgtttt tggatttcgg agaatgccat
       61 cgcccagctt tcatcacaca taaaaaacag tgcgggcacg gcttttttca gcggtatttc
      121 tttcaggtgc agggcaagtg ccgcccccat caggatatgc cgcgaattaa tcataaaggt
      181 tacggtggcg ataagcagta tcggcagaga ttccgcccac aggttgaccg tggcaaactc
      241 ggagccgccg gcgaagttca tactggtcat caacaacatt tccagccagc tcatgccttt
      301 ttgtccgccc tgcataccga gtattaatgc ccaaggcaga agtccgataa gtatgggcga
      361 actttctttg atgccgcgta taaattcgtt acggggcgag cttatgtgtg tcatattata
      421 atattgatat gtaagaaggt cgggcattat acacagacgg agtatttcaa aacaaaacct
      481 atttgctgtg tttcagtgta aacacggctt gtgtataatc caccatcttt agacagaccg
      541 accgtatgtg ggagcaaggc aatgaatatt gaagtagaaa tgaaagtgtt ggacgagagg
      601 atggcggatt ttatccctac ctatgcgaca gcgggttctg ccggtttgga cttgcgcgcc
      661 tgtttggatg aggaagtcgt cttgcagccg ggggaaacgt ttcttgtgcc gacaggtttg
      721 gcaatttatt tggccaatcc ttcatatgcc gccgttttgc tgccccgttc cggcttgggg
      781 cataaacacg gcattgtttt gggcaatttg gtcggcttga ttgactccga ttatcaaggg
      841 gaattgaagg tttcgttatg gaacagaagc agcgagccgt ttactgtcaa accgtttgag
      901 cgtatcgcgc agatggttat cgtgccaatc gtgcaggcgc gtttcaaacg tgtcgaggaa
      961 tttgtcggaa gcagccgtgg agaaggcggt ttcggcagta cgggtttgca ttgaatataa
     1021 aatgccgtct gaagggcacg tcaggttcag acggcatatc ttccgatata ccgataaccg
     1081 gagaaaatca tgaatacctt actcaagcag ctcaaaccct atccctttgc ccgactgcac
     1141 gaggcgatgc agggcatttc cgcgcctgaa ggcatggaag ccgtccccct gcacatcggc
     1201 gaaccgaaac atccgacacc gaaagtcatt acggatgcgc tgaccgcctc attgcacgag
     1261 ttggaaaaat atccgctgac tgcgggtctg cctgaactgc gtcaggcgtg tgcaaactgg
     1321 ttaaaacgcc gttacgatgg cttgacagtg gatgcggata atgaaattct gccggtttta
     1381 ggcagtaggg aggcgttgtt ttcttttgtt caaaccgtgt tgaaccctgt ttcagacggc
     1441 atcaaacccg caattgtcag cccgaatccc ttttatcaga tttacgaagg tgcgacactt
     1501 ttgggcggcg gtgaaatcca ttttgccaat tgccccgcgc cgtctttcaa ccccgattgg
     1561 cgtagtattt ccgaagaggt ttggaaacgc accaaactgg tgttcgtctg ctcgcccaac
     1621 aaccccagcg gcagcgtgct ggatttggac ggatggaaag aagtttttga tttgcaggac
     1681 aaatatggtt tcattattgc ctcggacgaa tgctattccg aaatctattt cgacggcaac
     1741 aagcctttgg gctgcctgca ggccgctgca cagttggggc gaagcaggca aaaactgctt
     1801 atgttcacca gtttgtccaa gcggtccaat gttccaggcc tgcgttccgg ttttgtcgcc
     1861 ggcgatgccg aactgcttaa aaattttctg ctttacagaa cctatcacgg cagcgcaatg
     1921 agcattcccg tgcagcgcgc aagcattgcc gcttgggatg acgaacagca cgttatcgac
     1981 aaccgccgcc tgtatcagga aaaatttgag cgcgttattc ccattttgca acaggtattt
     2041 gacgttaaat taccggatgc ctcgttttac atctggttga aagtccccga tggcgacgat
     2101 ttggcatttg cacgcaattt atggcaaaaa gccgctatcc aagtattgcc cggacgcttt
     2161 ttggcacggg ataccgaaca gggcaatccc ggggaaggtt atgtccgtat cgctttggtt
     2221 gccgatgtcg caacttgtgt caaagctgcg gaagacatag tttcactgta tcggtaagga
     2281 atcggaaatg ccgtctgaac ttttgttcag acggcatttt tcaatattct acggttgaat
     2341 ttgctgtaac ggtatttata gtggattaac aaaaatcagg acaaggcgac gaagccgaag
     2401 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttaaatt taatccacta
     2461 tacttcactt ttaatcggct tgcccgcaaa cacgtttaaa cttaaaatcc ccgtgtttga
     2521 cacaataccg agcagattat gttttttgtc ctttcccctg cgaagaacct taatgaaaaa
     2581 gaccctgccc ctgtcagcga gtttacccaa cccgacctgc tggcagagtc cgacattcta
     2641 atgcagcagt tgcgcgagct tgcgccgcaa cagattgccg aactgatgca cgtttccgac
     2701 aaaattgccc tcttaaacgc gcagcgcaat gcagaatgga acacgccgtt tacgccggaa
     2761 aacgccaaac aggcggtctt tatgttcaac ggcgatgttt acgaaggtat ggatgcaaac
     2821 acattgaata ccgatcaaat acaatatctg caagggcgcg tacgcctgct gtccggcctg
     2881 tacggccttc tgcgtccact ggacctgata cagccttacc gtttggaaat ggggacggca
     2941 tttgccaatt tgcgcgggaa gaatttgtat gagttttggg gagacatcat taccaacctt
     3001 ttaaatgaca cgcttgccca agcaggcagc aatacgcttg tcaaccttgc ctcacaggaa
     3061 tatttcaagt ccgtcaacac gaaaaaactt cgggcgcggc tgattacccc gatatttaaa
     3121 gacgaaaaaa acggcaaata taaaatcatc agtttctatg ccaagcgcgc gcgaggatta
     3181 atggtgcgct atgcggcaga acacaatatt accgatcctg aaatgctgaa aaattttaat
     3241 tacgaaggct acgcatttaa tgacgcggct tcaaatgaaa gcgaatgggt gtttatgcgt
     3301 tcggaacaaa taaagtgaaa acaataaatt aagtatttcc caaaaaaagt gcttggcaaa
     3361 atgtataaac ttcattatta ttcctactct tcaagaagac ggaagcgtgg cagagcggtt
     3421 taatgcaacg gtcttgaaaa ccgtcgaggg ttgatagccc tccgtgagtt cgaatctcac
     3481 cgcttccgcc aatccttgag gtcaaaaacc aaataaaaat gaagtagcat aaagcatcgg
     3541 catttttatt tggttttact tcatcgtgtt tccttttcgg ttgaacccca ccctttaggg
     3601 cggtagaatc agactttatt tgggaggggt gtaacccttt ctgaatcagg gtaacacata
     3661 gggcggtgct ttatgtgtcg tcctgtgtgt tgaaacatct ttcggaaacg tggcagagag
     3721 gctgaatgca gcggactcga aatccgctga gggtgcaaat cctccgtggg ttcgaatccc
     3781 accgtttccg ccacaaaaca aaaccgccct gattcggggc ggttcttttt tgttca
//
LOCUS       DAWKOD010000171         3753 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000171 DAWKOD010000000
VERSION     DAWKOD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.171"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..906)
                     /locus_tag="WJK73_001866"
     CDS             complement(88..906)
                     /locus_tag="WJK73_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7558302.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WJK73_001867"
     CDS             complement(986..2236)
                     /locus_tag="WJK73_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7558303.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPSRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFSLSPVSGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSSGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJK73_001868"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJK73_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ7558304.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WJK73_001869"
     CDS             2896..3708
                     /locus_tag="WJK73_001869"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ7558305.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          836 a          901 c         1113 g          903 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatacagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaagg gatttcctat ttttggggct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttggataa aggcgtgtag tctgtcccgc ttatagtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttccg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggatcg gggcggtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacgcc cgaggcaatg gattgggcgc actgccgacg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gcgcgcgcaa aacggcgaaa ttggcttcgt ccactctgcc tttgtgcaag tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attattttgg gtttgttcca agaatttggc ggcagagctg gtcgcgcctt
     1381 tggtgtttaa catataaacc cccgtgccgc gcgcggaggg gctggtgaag gcatcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaaag
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgagctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttaagca gcgccatcat cggatcgtcg ggatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt tcttaaagcc gctgacagga gagagggaaa
     1861 agacttgtgc gtgggtgggc tgtttcaggt cgatgacgag gcggacggtg gtcggtgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatatcgac gaccagcctg cttgggttgt
     2041 cgagcgcgaa gtgctggtat ttgagcgcgg cggtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgatgg ccatatccgt gcggcagtga attgtggtgc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggcgtaaa
     2341 ttccccgccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 caatccggca tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtag gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccttcgaga taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cggattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtacgcgcg ctgatggagc ggctgccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc taccatcgaa
     3061 aatttctcga tgcagattgt cgatttttta ttggaacacg atgttaaggc gatggttatc
     3121 gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac gggcaatatg
     3181 cctgtgttgg acgtgatttc cgccggcgcg aaagccgcgc tggcaacgac gcgcaacaat
     3241 aaaatcggca ttatcgccac caataccaca gttaacagca atgcttatgc gcgcgccatc
     3301 cacaggaaca accccgatac gctcgtccgc acgcaggccg cgccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt tcccttgctc
     3481 aagcccttaa tcggcaggga ggcgggcaat gtcgcgttgg ttgattctgc aattacaacg
     3541 gccgaagaaa ccgcacgcgt ccttgctcag gaaggattgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa cgatgacgga
     3721 aagctgcccg agattacaga aacctaaaat ccc
//
LOCUS       DAWKOD010000172         3748 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000172 DAWKOD010000000
VERSION     DAWKOD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.172"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            374..3577
                     /gene="nalP"
                     /locus_tag="WJK73_001870"
     CDS             374..3577
                     /gene="nalP"
                     /locus_tag="WJK73_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ7558306.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPNLHT
                     GDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSTGNDAQAQPNTYALLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTVIEGGSLVLYGNNESDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNNLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1012 a         1019 c         1000 g          717 t
ORIGIN      
        1 cggagccgca gacagtacaa atagtacgga accgattcac ttgttaaaga atcgttctct
       61 ttgagctaag gcgcgacaac gccgtactgg tttttgttca tccactataa ctagggaaat
      121 tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta taaacaaaat
      181 aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa tataaaaacg
      301 ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt gtcatattca
      361 aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca aaccggctgc
      421 catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg gaggcggcac
      481 ttctgcgccc gacttcaatg caggcggtac cggtatcggc agcaacagca gggcaacaac
      541 agcgaaatca gcagcagtat cttacgccgg tatcaagaac gaaatgtgca aagacagaag
      601 catgctctgt gccggtcggg atgacgttgc ggttacagac agggatgcca aaatcaatgc
      661 ccccccgaat ctgcataccg gagactttcc aaacccaaat gacgcataca agaatttgat
      721 caacctcaaa cctgcaattg aagcaggcta tacaggacgc ggggtagagg taggtatcgt
      781 cgacacaggc gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca gaaaagaaca
      841 cggctataac gaaaattaca aaaactatac ggcgtatatg cggaaggaag cgcctgaaga
      901 cggaggcggt aaagacattg aagcttcttt cgacgatgag gccgttatag agactgaagc
      961 aaagccgacg gatatccgcc acgtaaaaga aatcggacac atcgatttgg tctcccatat
     1021 tattggcggg cgttccgtgg acggcagacc tgcaggcggt attgcgcccg atgcgacgct
     1081 acacataatg aatacgaatg atggaaccaa gaacgaaatg atggttgcag ccatccgcaa
     1141 tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt ttggaacaac
     1201 atcgagggca ggcactgccg accttttcca aatagccaat tcggaggagc agtaccgcca
     1261 agcgttgctc gactattccg gcggtgataa aacagacgag ggtatccgcc tgatgcaaca
     1321 gagcgattac ggcaacctgt cctaccacat ccgtaataaa aacatgcttt tcatcttttc
     1381 gacaggcaat gacgcacaag ctcagcccaa cacatatgcc ctattgccat tttatgaaaa
     1441 agacgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag aaaagttcaa
     1501 tggctccaac cattgcggaa ttactgccat gtggtgccta tcggcaccct atgaagcaag
     1561 cgtccgtttc acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat
     1621 cgtaaccggc acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct
     1681 gcgtaccacg ctgctgacaa cggctcagga catcggtgca gtcggcgtgg acagcaagtt
     1741 cggctgggga ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg
     1801 cgactttacc gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat
     1861 ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa
     1921 cacctatacg ggcaaaaccg ttatcgaagg cggttcgctg gtgttgtacg gcaacaacga
     1981 atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg
     2041 cagcctgaac agcgacggca ttgtctatct ggcagatacc gaccgatccg gcgcaaacga
     2101 aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt acacacgttt
     2161 gggcaaactg ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatgtcggc
     2221 acgcggcaaa ggggcaggct atctcaaccg taccggacaa cgtgttccct tcctgagtgc
     2281 cgccaaaatc gggcgggatt attctttctt cacaaacatc gaaaccgacg gcggtctgct
     2341 ggcttccctc gacagcgtcg aaaaaacagc gggtagtgaa ggcgacacgc tgtcctatta
     2401 tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg
     2461 tctgaaacac gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga
     2521 tgcctccgaa tcatccgcaa cacccgagac ggttgaaact gcggccgccg accgcacaga
     2581 tatgccgggc atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc
     2641 gaatgccgcc gacggtgtac gcatcttcaa caatctcgcc gctaccgtct atgccgacag
     2701 taccgccgcc catgccgata tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga
     2761 ccacaacgct acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga
     2821 acagggcggt gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa
     2881 aaccggcgaa aatacgacag cagccgccac actgggcatg ggacacagca catggagcga
     2941 aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc
     3001 gggcgatatc ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag
     3061 ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct
     3121 gggcgcactg ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg
     3181 cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg
     3241 ctggagcggc aacagcatca ctgaaggcac actggtcgga ctcgcgggtc tgaagctgtc
     3301 gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa
     3361 cggacgcgac tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac
     3421 gggggcacgc aatatgccgc acacccgcct ggttgccggt ctgggcgcgg atgtcgaatt
     3481 cggcaacggc tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa
     3541 ccacagcgga cgagtcggcg taggctaccg gttctgacgg acaggaagca gacagccgca
     3601 aagatcacgg tctttgcggc tgtttcttat gaaaagaaga ccctattcca attgcctgct
     3661 tctattgttt caagacttct tccaaagatt cggcattaat cagatgtata gcggattaac
     3721 aaaaatcagg acaaggcggc gaagccgc
//
LOCUS       DAWKOD010000173         3700 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000173 DAWKOD010000000
VERSION     DAWKOD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.173"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            261..1382
                     /gene="dnaJ"
                     /locus_tag="WJK73_001871"
     CDS             261..1382
                     /gene="dnaJ"
                     /locus_tag="WJK73_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ7558307.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1577..3595
                     /locus_tag="WJK73_001872"
     CDS             1577..3595
                     /locus_tag="WJK73_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ7558308.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          774 a          830 c         1110 g          986 t
ORIGIN      
        1 gaagtctgca gtcgcatcaa atgccgtctg aacatgcgtt cgggcggcgt ttttataaca
       61 aaaacacttc atggcggttg gttttatgcc tgtctaagtt tttgtgtcgt gcatacctga
      121 agatttcaga cggcatcggt ttatgctgtc tgaaaagtgt attccgtttc agtttgtaag
      181 ctatggcagt ctgtttgtct tgtgttttgc gcaattgccc ttattttgag ccgtgatttt
      241 attttgaatt agatgaaaaa atgagtaatc aagattttta tgcgacgctg ggtgtggcaa
      301 gaacagctac cgatgatgag attaaaaaag cctaccggaa attggcgatg aaataccatc
      361 ccgaccgcaa tcctgacaat aaagaggcgg aagagaagtt taaagaagta caaaaggcgt
      421 atgaaacttt gtccgacaag gaaaagcgcg ctatgtacga ccagtatggt catgcggcgt
      481 ttgaaggcgg cggacagggg ggcttcggag ggtttggcgg atttggcggt gcgcagggtt
      541 ttgactttgg ggatattttc agccaaatgt ttggaggcgg ttcggggcgc gcccagcctg
      601 attatcaggg tgaggacgtt caagtcggta tcgaaatcac gcttgaagaa gccgcaaaag
      661 gtgtgaagaa acgcatcaat attccgactt atgaagcgtg tgatgtctgt aacggcagtg
      721 gcgcgaaacc ggggacatcc ccggaaacct gcccgacttg caaaggttcg ggtacggtgc
      781 acatccagca ggcgattttc cgtatgcagc agacttgtcc gacctgccac ggtgcgggca
      841 aacacattaa agaaccttgc gtcaaatgcc gtggcgcggg gcggaataag gcggtcaaga
      901 cggtggaagt caatattccc gccggtatcg atgacgggca gcgtatccgt ttgagcggcg
      961 aaggcggacc gggtatgcac ggtgcgcctg ctggcgacct gtatgtaacc gtccgcattc
     1021 gggcgcataa gattttccaa cgcgacggtc tggacttgca ttgcgaactg ccgatcagtt
     1081 ttgccacggc tgctttgggc ggggagttgg aagtgccgac cttggacgga aaggtcaagc
     1141 tcaccgtccc caaagaaacc caaaccggca ggaggatgcg cgtgaagggt aagggtgtca
     1201 aatctttacg cagcagcgcg accggcgatt tgtactgcca tattgttgtc gaaacgcctg
     1261 tcaatttgac cgaccgtcaa aaagagcttt tggaagaatt tgagcggatt tctaccggct
     1321 tggaaaacca aacaccgcgc aagaaatcgt ttttagacaa gctgcgcgat ttgtttgatt
     1381 gattttaagg ttcggaaaca agcagccgta tcggggaatc tccctgatac ggctgttttg
     1441 tttgtttaaa aatagttttt attttcaatg gggtatgggg cagggtggga taactgtttt
     1501 taactgttct ttttaaaact tgacatcatg gcgcgatgcc aacaatatgt gaacgtctgt
     1561 tgtcaaagga agaataatga ataaatcttt atccgggtcg gtagaagaat accgcgaact
     1621 gacgctccga ggcatgatac tcggtgcatt gatcactgta atttttactg cgtccaacgt
     1681 ttacctcggt ttgaaagtcg ggctgacctt tgcctcgtcg attccggcgg cggtaatttc
     1741 gatggcggtt ttaaagtttt tcaaaggcag caatattttg gaaaacaaca tggtgcagac
     1801 ccaagcttcg gcggcgggta cgctttcgac catcatcttc gtcctgcccg gtttgctgat
     1861 ggcgggctac tggagcggtt tcccgttctg gcagacgacg cttttatgta ttgccggcgg
     1921 gattttgggg gtgattttca ccattccgct gcgttacgcg atggtggtga aaagcgattt
     1981 gccttatccg gaaggtgtgg cggctgctga aattttgaaa gtgggcggtc atgaagaagg
     2041 ggataaccgt cagggcggca gcggcatcaa agagctggcg gccggcggtg cgttggcggg
     2101 attgatgagc ttttgcgccg gaggtctgcg cgtgattgcc gacagcgcga gttattggtt
     2161 taaaagcggt acggcgattt tccagctgcc gatgggcttt tcactggcat tgttgggcgc
     2221 gggctatttg gtcggactga cgggcggtat cgccatcctg ttgggcattt cgattgcttg
     2281 gggcattgcc gtgccgtatt tctcctcaca cattccgcaa ccttccgata tggaaatggc
     2341 ggcgtttgcg atgaagctgt ggaaggagaa agtgcgtttt atcggtgcgg ggactattgg
     2401 cattgcggcg gtttggacgc tgttgatgct gctcaagccg atggtggaag gcatgaagat
     2461 gtcgttcaag agttttggcg gcggtgcgcc cgctgcggaa cgcgccgaac aagatttgtc
     2521 gcctaaagcc atgatttttt gggtgctggc gatgatgttt gttttaggcg tgtcgtttta
     2581 ccactttatc ggcgattcgc acattacggg cggcatggct tggcttttgg tggtcgtttg
     2641 cacgcttttg gcttccgtca tcggcttttt ggtcgccgcc gcctgcggtt atatggcagg
     2701 tttggtcggc tcgtcttcca gcccgatttc cggcgtgggc atcgtgtccg tcgtcgttat
     2761 ttcactggtt ttgctgctgg taggcgaatc cggaggtttg ttggcggatg aggctaaccg
     2821 caaatttttg ctggcactga ctttgttttg cggctcggca gtaatctgcg tggcttcgat
     2881 ttccaatgac aacctgcaag acttgaaaac cggctacctg ctcaaagcca cgccttggcg
     2941 gcagcaagtc gccctgatta tcggctgtat cgttggtgcg ctggttattt cgcccgtgtt
     3001 ggaactgctt tacgaagcct acggctttac cggcgcaatg ccgcgcgaag gcatggatgc
     3061 ggcgcaggct ttggcagctc ctcaagcgac tttgatgacg accatcgcgt cgggcatttt
     3121 cgcccacaac ctcgaatggg tctacatctt taccggcatc gcgatcggcg cggtattaat
     3181 cgtcgtcgat ttggtgttga aaaaatcatc aggcggcaaa cttgccctgc ccgtccttgc
     3241 ggtcggtatg ggtatttatc tgccgccgtc cgtcaatatg cccatcgtgg caggcgcggt
     3301 gttggcggcg gtgttgaaac acatcatcgg taaaaaagcg gaaaaccgcg aaggccgtct
     3361 gaaaaacgcc gagcgtatcg gaaccttgtt ctccgccggc ctgattgtcg gtgaaagcct
     3421 gatcggtgtg attatggcgt ttattattgc cttctccgtg accaacggcg gctcggatgc
     3481 gccgcttgcg ttgaatctgc aaaactggga tgccgccgct tcttggttgg gtttggcgtt
     3541 cttcgttacc gggatgtttt tctttgcaca gcgcgtactg aaggcgggca agtaggctgt
     3601 cggaaaaaat gccgtctgaa gcgttcagac ggcattttta tctggaaagc ggaaggcgga
     3661 gcttttcggc ttgcgcccac gttttgccgg caaggtcttt
//
LOCUS       DAWKOD010000174         3659 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000174 DAWKOD010000000
VERSION     DAWKOD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.174"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..278)
                     /gene="rpmG"
                     /locus_tag="WJK73_001873"
     CDS             complement(123..278)
                     /gene="rpmG"
                     /locus_tag="WJK73_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ7558309.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(310..543)
                     /gene="rpmB"
                     /locus_tag="WJK73_001874"
     CDS             complement(310..543)
                     /gene="rpmB"
                     /locus_tag="WJK73_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ7558310.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(793..2319)
                     /gene="farB"
                     /locus_tag="WJK73_001875"
     CDS             complement(793..2319)
                     /gene="farB"
                     /locus_tag="WJK73_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ7558311.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2343..3497)
                     /gene="farA"
                     /locus_tag="WJK73_001876"
     CDS             complement(2343..3497)
                     /gene="farA"
                     /locus_tag="WJK73_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ7558312.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT          901 a         1023 c          917 g          818 t
ORIGIN      
        1 tctctatcct tggcagacgg tttgcagcag attcgcatac ggcagatgtt tcaagcggaa
       61 aggaaacacg ataaaaataa caaagcctcc gagcagtcgg aggctttgct ttcaaactga
      121 aattatttca gtttagtttc tttatacact acgtgtttgc gggcaactgg gtcaaatttt
      181 ttgatttcca atttgccggg catagtgcgt ttgtttttgg tagtggtgta gaagtgacca
      241 gtacctgcac tggattccag tttgatttta tcgcgcattg cagtaatcct taattaaata
      301 gtgtttaaat taagcttcgc cgcgagcacg caaatcagcc aatacgacat caatgcctac
      361 tttgtcgatg gtacgcagtg cagcgttgga aacgcgcagg cgaacccagc ggttttcact
      421 ttctacccaa aaacgacgtg attgcaagtt gggcaaaaaa cggcgtttgg ttttgttgtt
      481 ggcgtgcgat acgttgttgc cggacatcgg gcgtttgccg gtcactttgc aaactcgtgc
      541 cataatcagt ctccaaactt ctaaaatagt aaaacgcgat ttataccacg aaaaacagtg
      601 ttagttcaag tgttttaaag aaaaacggct gttttgcgcg gataatcctt ccgattgtcg
      661 catctgtgga acgtttgcag gtgcgaaaca gatggggcgc gattatagcg gatttcggga
      721 actgctgcat tcaaaatgcc gtccgaacat atttccagta ttttcagacg gcatttcaag
      781 ttttcaaatc cctcaatgtc caccgccgcc gccgttgtgg aacggcggtt ttgccagcca
      841 tatgacgggt atcatgataa tgaataacaa gctgcccgcc ataaagattt cgttcgagcc
      901 gataataaaa ccctgctggg taatggtatt gttgatgatg cctagggttt gaatgtcgga
      961 aacgccgtgc tgggacaaat gagcggccgt ttcgtgcaat gttgcggaat agggcgtgat
     1021 gtgttcggca aagcgcgtgt ggtgcaacgc ttcgcgccgt tcccacaggg tgctgacgac
     1081 ggatacgccg acaccgccca tcagcacgcg caggaaattc gacaggctgc cggcggcggc
     1141 aatctgcccg cccttcatgt gcgacagtgt gatggtagtc agcggcagga agaacatggc
     1201 aacgcctact ccctgccaaa actgcggcca gatgacgttg ccgatatcca tgtcggcata
     1261 aaaatcggtg cgccaataga aagtgaaggc aaaggtcagg aagctggcgg ttacgagcag
     1321 gcgcatatcg attttattgc cgaacctgcc gattaacgga gacaggaaga cgggcaggat
     1381 gccgacgggc gcggcggcaa gccccgccca cgtggaggta tagcccaggt tggtctgcaa
     1441 cactaacggc agcagggtca gcgtccccat atacaccata aaacccaatg atgtggcaat
     1501 gatgccgacg gtaaaattcc gatctttaaa cagcgataaa tcgacaatcg gatatttttc
     1561 tcccaattcc caaacaataa aatacgacaa gcacaccagt gcgactacgc ccaaggtaat
     1621 gatttctcca gaggcgaacc agtcgagttc cttacccctg tccaacatca tctgtaacgc
     1681 gccgataccg actaccatca atgtaagccc gacatagtcg gtcggcattt taacggtttc
     1741 cgtttcccga catttcaaat gtttccatgt aatccatgcc gatatgatac cgatagggat
     1801 attaatgaag aaaatccaac cccaatgcca gtttccggaa atccagccgc cgagtatcgg
     1861 cccgagaaca ggggcgacaa cgacggtcat tgcccacaat gccagtgcca gcgtccgttt
     1921 tgcgggcgga taggatgcca ttaacaggct ttgcgacaag ggaatcagcg gcccggcgat
     1981 aaagccctgc aagatgcgga aaacaaccag cgactgaagg ttgggggcaa taccgcacag
     2041 ccacgatgtg atgacgaaac cgacagcggc ggcggtaaac aatttgacct cgccgatacg
     2101 ttttgccaaa aagcccgtta acggcacgga aacggcgttt gccacagcaa aagaagtaat
     2161 gacccacgtc ccctgcgtgg ttgccgcacc gaggttgccg gcgatgacgg gaacggcgac
     2221 attggcgata gtcgtatcta aaacttccat aaatacggca agccccaaag acagcgtaac
     2281 ccacgccaat gccgcaccct taagcggtgg ataatccatt gtgtcctccg tttcagacgg
     2341 catcagggcg cggattgccc gaggatttca tcgaccgtcc gatcgacttc cgaccagtcg
     2401 gtactttcca tttccggcaa tgccgcaccc ggcgtttttg aaacaggcgc gcctgcggcg
     2461 gaagtatcca ctttaaccgt catcgacaaa ccgatacgca acggatgcct gtccacatct
     2521 tcgcggttca gcacgatacg gacggggacg cgctgcacca ctttaatcca gttgcccgtt
     2581 gcgttttgcg ccggaatcag cgaaaacgcg ctgcccgtac ctgccgaaaa acctgccacc
     2641 ctgccgcgat aaacaatttg tttgccgtac aaatcggaca ccagctcggc aggctgtccg
     2701 attttcatat gccgcaactg cgtctcttta aaattagcat ccacccacac atccgacagc
     2761 ggcaccaccg ccatcagcgg cgcgcctgcc gccacctgct gcccgacctg caccgaacgc
     2821 ttcgccacct gaccgtccgc cggcgcgcgg atttgcgtcc gctgaaggtt caaccacgca
     2881 tctttcaacc tgccgattgc cgtctgaacc gccggctgtt cgcgcaaaga aacctgaccg
     2941 cccaaagccg cacgtgccga agattcttcc gccaaagccg ctttgaccgc cgcctgcgcc
     3001 tgagacactg ccgcacgggc gtgtgccagc tcttcggcgg acaccgcgcc cgattccgcc
     3061 aaagcagacc ggcggcgcaa atcatcctgt gcgcgtgcca aatccgcccg gcgcaaggca
     3121 acctgcgccc ccgcctgcga agtggcggca ttttgccggc ggttttgccg caccgcctga
     3181 accagctcgt tttttgcccg ctcgtaagcc agcacatcat tatcgtcgtc caataccgcc
     3241 agcacgtcgc cttttttcac ggcatccgta tcgtcgtgca aaaccttccg caccgtaccg
     3301 cccttttgcg gcgtaacctg aaccacgcgt ccggcaacat aagcgtcttc cgtttcctct
     3361 tcgtgctgcc accataaaaa aaacgccgac ccggcggctg cggcggcaag cgcgaacagc
     3421 agcgtcaatg ccgtcaggcg gcgtttgcgt ttggcttgcg tgttttgaag ttttgtttcg
     3481 tccgtgtgcg tatccataag attgggttcc gaatttcctg atttcatata aacaatgttc
     3541 tttcaatagg aagcagattc tatatccgcg caataacgca atcaagtcag tattttagat
     3601 gcaatattca aaaaacaacg cttcaggcgg caaaactgaa aatatatttc gtttatata
//
LOCUS       DAWKOD010000175         3622 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000175 DAWKOD010000000
VERSION     DAWKOD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.175"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            369..3462
                     /locus_tag="WJK73_001877"
                     /pseudo
     CDS             369..3462
                     /locus_tag="WJK73_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
BASE COUNT         1002 a          931 c          963 g          726 t
ORIGIN      
        1 aaccgctccg ccgtcattcc cgcgcaggcg ggaatccaga ctgtcgggca tctgcagcgg
       61 tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt aaaatgggga ggtaagcttt
      121 tcaaccagca attcggcggg cgcgggatcg ggcggtttgc cgaatcccgc cgtttgcggc
      181 gcgcctgccg ccgacggtat cccgcgaagc aagatttaag ggataaaata tgttccaaca
      241 cgcagggcgg cacataaggc gccgccctga ttcggaaggg cttgcacccc tcccggacaa
      301 agcctgatcc tgccgccccg aaggacggat gcccgaaggg cggggggttt gaccgaaaag
      361 gaaatacgat gaataaaact ttaaaaaggc aggttttccg ccataccgcg ctttatgccg
      421 ccatcttgat gttttcccat accggcgggg gggggggcga tggcgcaaac ccataaatac
      481 gctattatca tgaacgagcg aaaccagctc gaggtaaagg ggaatgggca atattcaaca
      541 ataaaggaca aagacaggga acgcaaattt atctataata aagacagagg gggtggaggc
      601 tctgtctttt tcgacaatac cgataccctt gtttcccaac aaagaggtac tgccgttttt
      661 ggcacagcca cctacctgcc gccctacggc aaggtttccg gttttgatgc cgacgggctg
      721 caaaagcgca acaatgccgt cgattggatt cataccaccc aggccgggct ggcaggctac
      781 gcctacaccg acgtcatatg cagaagcaac caatgccccc aacttgtcta tgagaccaaa
      841 ttttccttcg acggcatcgg tttggcaaaa aatgcgggca gcctggatag gcacccggac
      901 ccaagccgcg aaaattcgcc catttacaaa ttgaaagatc atccatggtt gggcgtatct
      961 ttcaatttgg gcagcgagaa taccgtcaaa gatggcaaat cattcaacaa attgatatct
     1021 tcttttagtg aaggcaataa taatcaaacc atcgtctcta cgacacgagg ccactctatt
     1081 tcccttagcg actggaagcg cgaacatacc gccatggcct attatctgaa cgccaaactg
     1141 cacctgctgg acaaaaaagg gattgaagat atcgcccaag gcaaaacagt ggatttgggc
     1201 accttgagac cgcgcgtcga ggcaacggta aggcgggggg agctgctaaa tttttgggct
     1261 acgtggaaga ttgaagataa agggaacatt acagtccgcc tcggcctgcc ggaagtcaaa
     1321 gcaggccgct gcgtcaacaa agcgaacccc aatcccaacg ccaaagcccc ctcccccgca
     1381 ctgaccgccc ccgcgctgtg gttcggacct gtgaaagatg gtaaggcgga gatgtattcc
     1441 gcttcggttt ctacctaccc cgacagttcg agcagccgca tcttccttca aaatctgaaa
     1501 agaaaaaacg accccaacaa acccggccgc tattccctcg cagacttggc taagtcggat
     1561 attgaaaatc gacagccgaa tttcacaggg cggcaaacaa tcatccgatt ggatggcggc
     1621 gtacagcaga ttaaacttca aggcaatgag gtcgccaatt ttaatggaaa tgacggcaaa
     1681 aacgacactt tcggcattgt tagcgaaggg agcttcatgc ctgataccag cgagtggaaa
     1741 aaagtattgc tgccttggac ggttcggggt tttgctgatg acagtaaatt taaagaattc
     1801 aacaaagaag aaaaaaacaa cgacaacaag ccaaaataca gtcaaaaata ccgcagccgc
     1861 gacagcagca agcacgagcg cgatttgggc gacatcgtca acagccccat cgtggcggtc
     1921 ggcgagtatt tggctacttc cgccaacgac gggatggtgc atatcttcaa aaaaaacggc
     1981 ggcggggatg accgcaacta tagtctgaag ctcagctaca tccctggcac gatgccgcgc
     2041 aaggatattg aaagcaaaga ctccaccctt gccaaagagc tgcgcgcctt tgccgaaaaa
     2101 ggctatgtgg gcgaccgcta cggcgtggac ggcggctttg tcttgcgcga agtcgaacgg
     2161 ggcgggaaaa aacacgtgtt tatgttcggc gcgatgggct ttggcggcag aggtgcgtat
     2221 gccttggatt tgaccaaagc cgaaaacggt aacccgaccg ccgtttccct gtttgatgtc
     2281 aaaaatgacg ataaaaacaa taatggcgtg aaattaggct acaccgtcgg cacgccgcaa
     2341 atcggcaaaa cccacaacgg caaatacgcc gccttcctcg cctctggtta tgcgactaaa
     2401 gacgttaaca gcaacgacaa tacaaccgcg ctgtatgtat atgatttgga aagcagcggc
     2461 aatttgatta caaaaatcga agtacccagc ggcaagggcg ggctttcgtc ccccacgctg
     2521 gtggataaag atttggacgg cacggtcgat atcgcctatg ccggcgatcg cggcgggaat
     2581 atgtaccgct ttgatttgag aggcaacgac ccgaccaaat ggtctgcaca cgccattttc
     2641 agcggcaaca aaccgattac ttccgcgccc gccatttccc aactgaaaga caaacgcgtg
     2701 gttatcttcg gcacgggcag cgatttgagt gaggaggatg tactcagtac gagcgaacaa
     2761 tatatttacg gtatcttcga cgacgatacg gtggcgaata acgtaaatgt aaaactcagc
     2821 ggtttgggag gcgggctgct cgagcaagag cttaagcagg aggataaaac cttattcctg
     2881 accgattata agcgatccga cggctcgggc agcaaagggt gggtggtgaa attgaaggac
     2941 ggacagcgcg ttaccgtcaa accgaccgtg gtattgcgta ccgcctttgt aaccatccgc
     3001 aaatataacg acggcggctg cggcgcgcaa accgccattt tgggcatcaa taccgccgac
     3061 ggcggcaagc tgaccaagaa aagcgcgcgc ccgattgtgc cggccgacaa tacggctgtc
     3121 gcgcaatatt ccggtcataa gaaaggcatc aacggcaaat ccatccctat aggttgtatg
     3181 caaaaaggca atgaaatcgt ctgcccgaac ggatatgttt acgacaaacc ggttaatgtg
     3241 cgttatttgg atgaaaagaa aacagacgga ttttcaacaa cggcagacgg cgatgcgggc
     3301 ggcagcggta tagaccccga cggcaagcgt gccggcaaaa acaaccgctg cttctcccaa
     3361 aaaggggtgc gcaccctgct gatgaacgat ttggacagct tggacattac cggtccgacg
     3421 tgtggtatga aacgaatcag ctggcgcgaa gtcttctact gatttgcacg cgaaaatgcc
     3481 gtccgaaagg ttttcggacg gcattttttg cgtttttcgg gaggggcggg tttgtaaaag
     3541 gcgggctata gggtaggctt catctcgcca atctcactga atccatcaat ttccacaatt
     3601 caattaaata ccgtcaaacc ga
//
LOCUS       DAWKOD010000176         3574 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000176 DAWKOD010000000
VERSION     DAWKOD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.176"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..124)
                     /locus_tag="WJK73_001878"
                     /pseudo
     CDS             complement(1..124)
                     /locus_tag="WJK73_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1421..1870
                     /locus_tag="WJK73_001879"
     CDS             1421..1870
                     /locus_tag="WJK73_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558313.1"
                     /translation="MQPGSKNDEKNKITLNEFNNAIVTIFSDENVVRAIGTINTMHGE
                     YLTRSHKIDQQKFLSDGERLSSKAKDFQHSAEKTQRALKEGIGRAASKFHANSKEAFV
                     ARKMQSDLQKKLNASNYNKQQYARQAVTALENASKAENKGNIAFYLN"
     gene            2427..>3574
                     /locus_tag="WJK73_001880"
     CDS             2427..>3574
                     /locus_tag="WJK73_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7558314.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFA
                     GALFDHRNQGIRTATGWVAADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGY
                     AALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQANELRTLEELG"
BASE COUNT         1122 a          532 c          740 g         1180 t
ORIGIN      
        1 tggataccca aaatatcggc ggcagaacgg gcggtaattt ccagcgcaaa aaaaacggag
       61 cagttctttc tgtacttttt tctttaattt gcagtgtgtt atcttcatat ttcgagggta
      121 acatatctgc taatctagta cagccccaaa aatataccaa aaacagcaaa acaaattgta
      181 aggataggta taggctttgt aaaggtaaat tgtgaaaaaa gcagtttttt aaacgaatga
      241 aacggcttcg ggctgaaata tatgctgatg ccctgttctt cccgtatatc ttgtgtgttg
      301 tcaaagtgca ggctgctttg aaattggcat tacagtcgat gaaccaccat tttgctttat
      361 ttcagcgggc ttgagatgtg tataagaata ttgttttgaa taaatttaaa gaaaatgata
      421 atcgttattg acgattttta aaggaaagcg tagagtgcca attctatctt gtgtgataca
      481 cgaagcaaca aggaaaacta tctagaatgt ccttgtatgg gcatagatca tagatcattt
      541 ccaggctgcc tgtggttgtt ttaactttaa gatggagggg tttgcctaga acatcagtgc
      601 ttatctccaa gacagcctcc ccaatccctt gtgcctctta ataaagaagt gattaggaag
      661 taattagtgt gtgaaactat agttcctttt atagctttcc aataaaatta aagtatttaa
      721 aagcttgttt agttccaatt tgttaatttc tgtatctaaa taatgttcag aatagtatta
      781 ttttttaaat aaaccatgaa tagtacttca cattgtttgt cgtattattt agcttttaaa
      841 tttctaatgt gaaagatagt ttaatggatt tctttgattt tttttgcgca aggtaagtat
      901 aaaaaaattg caactgaaca aggtttagac cgtaaatatt ttatattcca ctatatcgta
      961 tgtaatttat ctgccatttc ttctttttgt ttaatgaagg ttatccatag ttatactatc
     1021 ctttgtttat taataatgat aattggcatt gcatttggat ttatcttgag aaatagggtt
     1081 ctatctatag taaaaaataa acaaaagttt ttatctgata tgtttccctt ggaaatgcct
     1141 ttcttttcta gagtatacaa aatacctatc ttggccagat tcttgaatag gggaatgaaa
     1201 tggctttatc tccaaaattt tttctgttta gttatgattt gtttctcgat atctctgatc
     1261 cttaatgaag aaagggggtg gatgccttat tttatctgct attggtttgg actttgttat
     1321 ccagcaaaca cttattacgt ttttttatcc agagattata aacgtatcta agttgtagtc
     1381 tcttaataat ataatatttt agttgatgga aggtgttgtc atgcaacccg gttctaagaa
     1441 tgatgaaaaa aataagatta cactaaatga attcaataat gccattgtaa ctattttctc
     1501 tgatgaaaat gtggttaggg caatcggtac aattaacaca atgcatgggg agtatttaac
     1561 tcgttcgcat aaaatagatc aacaaaaatt tttgagtgat ggtgagcggt tgtctagtaa
     1621 agcgaaagat tttcagcact cagcagaaaa aactcaaagg gctttaaaag aagggattgg
     1681 aagggctgct agtaaatttc atgccaattc gaaggaggca tttgttgcaa gaaaaatgca
     1741 aagtgatttg caaaagaagt taaatgctag taactataac aagcaacaat atgcaagaca
     1801 agcagtcaca gctttggaaa atgcttcaaa agcagaaaat aaaggtaata ttgcatttta
     1861 tcttaactaa actaaaatta gttttcttga gaggaaagta ttaatggatt tttttaattt
     1921 tttcgagcaa ggatatttta aaaaactcgc ggttgaaaat ggattaaata ctgaatattt
     1981 tatatttcaa tttatagtat gtaatctatt tagtattttc gttattattt taatgaattt
     2041 tataaaaaat tacgctgtgc ttattgtttt aatagtaatt gttagtatat tatttggact
     2101 aattttaaaa aataaaacta tgtctttaat aaagaataaa gaaaaatttt tatgtggttt
     2161 gtttccgttg gatgtgccga tttttaaata tacaatgcct agttatttta aacttataaa
     2221 taagaatatg aggtggcttt ttcacatgaa tgttttctgt ttatttttta tttgtttttc
     2281 aatatatcta attaataaag gagagaatca gtatattgct tattttagtt gttatttttt
     2341 cggacttact tatccattaa ttaattattt gatattatta cacagagaat ataaacgtat
     2401 ttagttctaa atttggaggt gctattatgt attcgaataa tgctataact aataatagaa
     2461 acaatattac actaggtgcg tatagggatg ctattatttc catattttct gatgaaaatg
     2521 tggttagggc gattggtacg attaactcaa tgtacgaaga atatttgacc cgttcgcaca
     2581 agatagatca aaaagaattt ttaagggagg gtgagcgtct gtctgctaag gcaagagaat
     2641 tccaacgcac agcagaagag gcacaaaaaa gcttaaaaga aggaattgga agagctgcta
     2701 gtaaatttcg tggtaattca aaggaggcat ttgttgcaag aaaagtacaa agtgatttgc
     2761 aaaaagcatt gaatgctagc aactataaca agcagcaata tgcaagacgt gcggcaacag
     2821 cgttagagaa tgcttcaaaa tcaaaagtta tggcagcgaa tttgggtaag tttacttatg
     2881 gggcaactgt tgccatggga actttgagta tgggaagtgc ttggtggaaa gccttggatg
     2941 gagaaggaaa ttggagtgat gttggtctct catcagcttc ttttttgggt gggctaatgg
     3001 gcgtccaatg gggtgcggct ttgggtgttg gggcttttgg tttacttact ggtggcgcag
     3061 gttggctagt agttggagct atacttggag gtggcgcagg agcttggtgg ggatcattag
     3121 gagctgaaaa agcttggaga tggtttgagg atttcttaaa tcttaaccgc agcgacaaat
     3181 accacgttta cgaccccctc gccctagatc tagacggcga cggtatagaa accgttgcca
     3241 ccaaaggctt tgcaggtgca ttgttcgacc accgcaatca aggcatccgc accgccacag
     3301 gttgggttgc tgccgatgac ggtctgcttg tgcgcgattt gaacggcaac ggcatcatcg
     3361 acaacggtgc ggaactcttc ggcgacaaca ccaaactggc agacggctct tttgccaaac
     3421 acggctacgc ggctttggcc gaattggatt caaacggcga caacatcatc aacgcggcag
     3481 acgccgcatt ccaatccctg cgtgtatggc aggatctcaa ccaggacggc atttcccaag
     3541 ctaatgaatt gcgtaccctt gaagaattgg gtat
//
LOCUS       DAWKOD010000177         3559 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000177 DAWKOD010000000
VERSION     DAWKOD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.177"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..974)
                     /locus_tag="WJK73_001881"
     CDS             complement(120..974)
                     /locus_tag="WJK73_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ7558315.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1098..1826)
                     /gene="rpsB"
                     /locus_tag="WJK73_001882"
     CDS             complement(1098..1826)
                     /gene="rpsB"
                     /locus_tag="WJK73_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ7558316.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2003..2236)
                     /locus_tag="WJK73_001883"
     CDS             complement(2003..2236)
                     /locus_tag="WJK73_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558317.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMRQQEQAEFEQQRAQRQKDSGTKP"
     gene            2314..2895
                     /locus_tag="WJK73_001884"
     CDS             2314..2895
                     /locus_tag="WJK73_001884"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ7558318.1"
                     /translation="MRNEEKRALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPADGVKQERKRGR
                     AKLHVPQFAGRKKRVHDLNLLLVPVVGMDRLGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            2956..3546
                     /locus_tag="WJK73_001885"
     CDS             2956..3546
                     /locus_tag="WJK73_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7558319.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          788 a          824 c         1022 g          925 t
ORIGIN      
        1 ttttgttttc agacggcctt tatattcggt cgtcttaaaa taggtaccgc aaacggtttt
       61 ctcaaaaaaa agcaccctga ttcgtttgga atccaggtgc tttctttttc ataagtgcct
      121 tacactttgg cggctgcggc tacttcggct gcgtagtcga caacggcttt ctcgataccg
      181 tcgcctactt tgtagcggac gaagctgacc acttcagtgc cgttttcttt ggcgaattgg
      241 gcaacagttt gatcggggtt catcacgaat gcttggccgt tcagagtgat ttcagccaag
      301 aatttgcgga tacggccttc aaccatttta gcggcgatgt cggcaggttt gccggaagcg
      361 atggcttgtt cagtgtagat gtggcgttct ttttcaacgg tttcagcatc tacttcggct
      421 tcgcttacgc attgtggttt agcggcaaca atgtgcatac cgattttgcg tgctacgtct
      481 tcagagcctt tgtactcaac caatacgcct tcggtagcca atgcgccgtg gatgtaggca
      541 accagttggt tggcagtgtc gatcacttgg aagcgacgga cagacatgtt ctcgcccaat
      601 ttggcgatga tggctttgcg ttctgcttca accagttcgc tcagttcttc aacagaagcc
      661 ggttttttct cggcagcagt tttagcaacg aagttggcga attctacgaa gccggcatct
      721 ttagcaacga agtcggtttc gcagtttact tcaaccaatg caccgacatt gccgttgatc
      781 gcgtaagcca atacgccttc ggcagcagta cggccggcca gtttaccggc tttcgcacca
      841 gatttaatac gcaggatttc ttcggctttg tcgaagttgc cttcagcttc aaccaaggct
      901 tttttgcatt ccatcatgcc caggccggta gcggcgcgca ggtcggcaac catttttgca
      961 gtaatttctg ccattttgaa tctcctagat ttttgggaac acggccattg ccgtgtttgg
     1021 aaatagtggc cgtctgaaac ggaattcaga acgggatttg agaaaagggg cataacgccc
     1081 ctcttctgtg taccgaatta ctcggcagca gcttcttggg cagcggctac agtttcttgc
     1141 agcgcttggt ttttgccttc caaaactgcg tcagcgatgc cgcggcagta caggcggatg
     1201 gctttggcgg agtcatcgtt accggggata acgtatttca cgccgtcagg gctgttgttg
     1261 gtatcgacta cggcgataac agggatgccc aatttttcag cctcaaccag agtacctttt
     1321 tggtagccgg tatcgataac gaaaatcgcg tcaggcaggc ctttcatgtt tttgataccg
     1381 cccaaagaac gttccagttt ttcaacgtcg cgttgcattt ccagaatttc ttttttgttg
     1441 aaaccgcctt cggcagcatt ttccaaggct gcggtttttt cttccaggcg tttgatggat
     1501 tgcttaacgg ttttgtagtt agtcagcata ccacccaacc agcggtaatc aacgaaaggc
     1561 ataccggcac gggtcgcttc ttcgcggatg atgtcgcggg cttggcgttt ggtacctacg
     1621 aacaatactg tacctttgtt ggcaaccaga cgacgtacgg cttcttgcgc ctcttggaac
     1681 atcggcaggg ttttttccaa gtttacgata tggattttgt tgcgcgcgcc gaaaatgtat
     1741 tgtgccattt tcggattcca gaaacgggtt tggtggccga agtggacacc ggcttcaatc
     1801 atctgacgca tagtaatttg agacattttg atttccttga aagggttaag ggcacacatt
     1861 caatcgcata gaacttgata ggcgatgcct attgtcaagc acccttcggc gaaagtgggc
     1921 ataagtttta aaaaataaaa gagtgtttcc gaaatggaaa actgacggat tatagcggat
     1981 aagaggccgt ctgaaaaggg attcagggtt ttgtgccgct gtctttttgc cgttgtgcac
     2041 gctgctgttc gaattctgcc tgttcctgct gcctcatcag gcggttgcgg cggcggacgg
     2101 ttacgacgaa gaccgtgaac acggtgggca gaaccgccca aaaaaccaaa taaatcaagg
     2161 cgcgcgcaat actcggctgc gcggcggaaa acatcacggc aacaaaaaga tagccgatgg
     2221 cgacaatgtg ccacattgtg tgcgtcctgt ttgaatgtta acaaaatcgt tataatcgca
     2281 acattctacc gcaatcctga aaggccgtct gaaatgagga atgaggaaaa acgcgccctg
     2341 cgccgcgaat tgcgcgggcg gcgttcgcaa atggggtggg acgtgcgggc ggcggcaacg
     2401 gtaaaaatca accgcctgct caaacgttat attaaaaaag ggcggaaaat cggcgtgtat
     2461 tggccgatgg gcaaggaatt gcgtttggac ggctttgtcc gcgcggcgca aaaacgcggt
     2521 gtaaaacttt atctgcctta tatcgaaccg cgttcgcggc ggatgtggtt tacgccgtat
     2581 cctgccgatg gagtaaaaca agaacgcaag cgcggtaggg cgaagctgca tgtccctcag
     2641 tttgcaggtc ggaaaaagcg tgtgcatgat ttgaacctcc tgcttgtgcc agtggtcggt
     2701 atggacaggc tgggctaccg cttggggcag gcaggcggct attacgatgc gacgcttgca
     2761 gcgatgaaat accgtttgca ggcaaaaacc gtgggcgtgg gctttgcctg ccaactggtc
     2821 ggcacgctgc cgcgcgaggc gcacgacctg ccgctggacg gttttgtgtc ggaggcgggg
     2881 atattgtgtt tttagccgtt cagacggcat tccgggcgga aatgccgtct gaaggcggaa
     2941 tattgggaga aaaggatgtc ggaaaagata atactgccgg tcttgtcatt gggcggcgtt
     3001 cggctcgaac cgttggatgt gcatcatgaa acgggtttgc gcgaggcggt ttgcgatggg
     3061 gaggtttgga agctgggggt aacgtccgcg ccccatccgg atagggtggc agactatatc
     3121 gggacagcat tggcaacgcg tttggcgttt gcggttgtcg atgaagaggc gggcagggtg
     3181 gtcgggacaa cggcgtatta tcattttgaa ccgcagattc cgcgtttgga tatcggattc
     3241 acatggtatg cggcatcggc acggcggaca cggataaaca cctgttgcaa aatcatgctg
     3301 ttggattacg catttgatgt tttggcttgc cgttgcgtgg gatggcgcac ggatattctg
     3361 aaccttgcct cgcaacgtgc catcgaacgg ctgggtgcgg aaaaagacgg cgtgctgcgt
     3421 atgcatatgc tccgtaaaga cggcagtgtg cgcgatacgg tcgtgtacag tatgctgagg
     3481 gaagattggt gcaaaaacag ggaaatcctg accggcaggt tggcagggta tggcgtgcag
     3541 gtgtgaggca gtattttca
//
LOCUS       DAWKOD010000178         3494 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000178 DAWKOD010000000
VERSION     DAWKOD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.178"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            226..1101
                     /locus_tag="WJK73_001886"
     CDS             226..1101
                     /locus_tag="WJK73_001886"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ7558320.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALVRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1110..2306
                     /gene="bamC"
                     /locus_tag="WJK73_001887"
     CDS             1110..2306
                     /gene="bamC"
                     /locus_tag="WJK73_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ7558321.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2363..3295)
                     /gene="pip"
                     /locus_tag="WJK73_001888"
     CDS             complement(2363..3295)
                     /gene="pip"
                     /locus_tag="WJK73_001888"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ7558322.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT          933 a         1158 c          776 g          627 t
ORIGIN      
        1 aggcaacacc gtactggttt ttgttaatcc actataaatc cgacggcacc ggcataaacc
       61 ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat ttccaccata cctttcggcg
      121 caaacgcccc acatccgcaa cccgccggat ttcacacctg cggtaaagta ggctacaatc
      181 ggaagcatta ccgcccccta caccacaact gataaaaggt tctgtatgtt acaaggtagc
      241 ctggttgccc tgattacccc gatgaatcaa gacggcagca tccattacga acaactccgc
      301 gacttaatcg actggcacat tgaaaacggc acggacggca tcgttgccgt cggcacgaca
      361 ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg tcatcgaagc cgtcgtcaaa
      421 cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg caaacaacac cgtcgaagcc
      481 atcgcccttt cccaagccgc cgaaaaagcc ggggcggact acaccctctc cgtcgtcccc
      541 tattacaaca aaccttcgca agaaggcatg taccgccatt tcaaagccgt tgccgaagcc
      601 gccgcgattc cgatgatact ctacaacgtt cccggcagaa ccgtcgtcag catgaacaac
      661 gaaaccatcc tgcgccttgc cgaaatcccc aacatcgtcg gcgtgaaaga agccagcggc
      721 aacatcggca gcaacatcga actcatcaac cgcgcgcccg aaggcttcgt cgtcctgtcc
      781 ggcgacgacc acaccgccct accgttcatg ctctgcggcg gacacggcgt tattaccgta
      841 gccgccaacg ccgcacccaa gctctttgcc gatatgtgcc gcgccgcgct tcaaggcgat
      901 atcgccctcg cacgcgagct caacaaccgg ctgattccga tttacgacac catgttctgc
      961 gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc tggtcagatg cgaaccgcac
     1021 gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg caaaagtccg cgccgccctg
     1081 aaagcctcag gacaacttta aatatagcca tgccgtccga accgttcgga cggcataacg
     1141 caacaaacac tttaatatcc atcacacagg atgacacgat gacccatatc aaacccgtca
     1201 ttgccgcgct cgcactcatc gggcttgccg cctgctccgg cagcaaaacc gaacagccca
     1261 agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact tgaagtccca cctgatttga
     1321 acaaccccga ccaaggcaac ctctaccgcc tgcctgccgg ttcgggcgcc gtccgcgcca
     1381 gcgatttgga aaaacgccgc acacccgccg tccaacagcc tgccgatgcc gaagtattga
     1441 aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca acgctggctt gtcgttgacg
     1501 gcaaatcccc cgccgaaatc tggccgctcc tgaaagcctt ctggcaggaa aacggcttcg
     1561 acatcaaatc cgaagaaccc gccatcggac aaatggaaac cgagtgggcg gaaaaccgtg
     1621 ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac agtcggtttg ggcggcatct
     1681 actccaccgg cgagcgcgac aaattcatcg tccgtatcga acagggcaaa aacggcgttt
     1741 ccgacatctt cttcgcccac aaagccatga aagaagtgta tggcggcaaa gacaaagaca
     1801 cgaccatgtg gcagccttcc gcttccgacc ccaaccttga ggccgctttc ctgacgcgct
     1861 ttatgcaata tttgggcgtt gacggacagc aggcggaaaa cgcatcggca aaaaaaccta
     1921 cccttcccgc cgccaacgaa atggcgcgta tcgaaggcaa aagcctgatt gtctttggcg
     1981 actacggcag aaactggcgg cgcaccgcgc tcgccctcga ccgcatcggg ctgaccgtcg
     2041 tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa agccccgaac gaaagcaatg
     2101 cagttaccga acaaaaaccc ggcctgttca aacgcctgct gggcaaaggc aaagcggaga
     2161 aacctgccga acagccggaa ctgattgtct atgcagaacc tgtcgccaac ggctcgcgca
     2221 tcgtcctgct caacaaagac ggcagcgcat atgccggcaa agacgcatcc gcactgttgg
     2281 gcaaactcca ttccgaactg cgttaaaccg ttcaaaacaa aagcagcttc caaacagaag
     2341 ctgctttttt gtgcggaact ttttacaaca accgaggaaa aatatcctca accgcctgaa
     2401 ccaacgcatc cgccaaaggc ggatcgaacg cacgatgccc tgcctgaacc accctcaatt
     2461 ccgcttccgg aaaggctttc gacagcgccc acgcactctg catcggcgta cacaaatcat
     2521 accgcccctg tacgataacg gtcgggatat gccgtatttt gccgatattg ttcaaaatcg
     2581 ccctatcgcc ctgcaaccag ccgccgttga caaaataatg gttttccaaa cgcgcgattg
     2641 ccagcgaggc ataagcatct tcatccactt cctccggctc gaaacggatc agatagcttt
     2701 cccaatccgc ccaagccttc gcggcagaca gacacacttc ttcatcttga tgaaacagca
     2761 atccgtgata cgcctcaatc agccggttcc gccgattttc agcaatcggc gcgacaaatt
     2821 tttgccattg ttccggataa atccggctca caccgcccgc ctcgttcagc cacaccgttt
     2881 cagacggtct gcacaaaaat atcccgcgca acaccaatcc ctttacccgt tccggatggg
     2941 tttgggcata agccagcgac aaagtgctgc cccacgaacc gccgaacacc agccatttcc
     3001 cgatacccag catttcacgg actttttcaa tatccgccac caaatcccaa gtcgtattgt
     3061 cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg ctggtcgatg atgacgatgc
     3121 ggaacacatc gggattgaaa aaaccccgac attcaggcga agcccccgcg cccggcccgc
     3181 catgtaaaaa aataaccggc acaccgtcag gattacccga ctcttcccaa taaatttgat
     3241 gaatttcaga tacctgcaag tatccgctat gaaaaggctg ttttatctca tacatcatct
     3301 tctcccatat tgcttctgac aatccgaaca tcccgacacg cgacatcccg cacaaaacac
     3361 aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata gtggattaac aaaaatcagg
     3421 acaaggcgac gaagccgcag acagtacaaa taatacggaa ccgattcact cgtgcttcag
     3481 caccttagag aatc
//
LOCUS       DAWKOD010000179         3482 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000179 DAWKOD010000000
VERSION     DAWKOD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.179"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            207..1679
                     /gene="pyk"
                     /locus_tag="WJK73_001889"
     CDS             207..1679
                     /gene="pyk"
                     /locus_tag="WJK73_001889"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ7558323.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1989..3383
                     /locus_tag="WJK73_001890"
     CDS             1989..3383
                     /locus_tag="WJK73_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7558324.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          909 a         1060 c          870 g          643 t
ORIGIN      
        1 aataaattta gcctaagtca taaaaccgtg taattttgcg ccaaaaaagg cggtttgcag
       61 gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc
      121 aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac
      181 ccgattgaga ccaaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
      241 gccacaacac taaaatcgtc gccacccttg ggccgggcag caacaacgtc gaactgttgg
      301 aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc cacggcacgc
      361 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc accggacagg
      421 aaatcgccat cattgccgac ctgcagggcc cgaaaatccg cgtgggcaaa atcgccggcg
      481 gcggcatcga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag
      541 gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg
      601 atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
      661 ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaagggc atcaacaaac
      721 gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
      781 cgattgccat cggttgcgac tatctcgcca tcagctttgt gaaatccgcc gaagatttgc
      841 acatcgcacg cgccaaagtc gaagaagaaa tgaaaggcag tacggccgtg cgccccggtt
      901 tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atcctcgccg
      961 gcgacggcat tatggttgca cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     1021 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     1081 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     1141 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     1201 tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa
     1261 aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa gcggtcagca
     1321 ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     1381 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc
     1441 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     1501 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg
     1561 aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc attaccagcg
     1621 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     1681 ccgccctgag tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac ggcatttttt
     1741 gcggcggcgc ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg caacatcgga
     1801 taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg
     1861 gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc
     1921 gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg
     1981 aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg
     2041 ccgccgcatc cgcacaagcc tccggctacc acttcggcac acagtcggtc aacgcgcaaa
     2101 gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg
     2161 ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca
     2221 gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga
     2281 aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca
     2341 acgacaatct gaccgtaggc ttgggcgtgt acgtcccctt cggttctgcc accgaatacg
     2401 aaaaagattc cgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg
     2461 aacctgtcgc cgcgtggaaa ctcaacgaac gccattcctt cggcgcaggc atcatcgccc
     2521 aacatacttc cgccgagctg cgcaaatatg ccgactgggg gattatggaa aaagcgaaag
     2581 cactaaaaga aacacccccc aatccaacta aagccgccca aatcaaagcc gacggacacg
     2641 ccgatgtcaa aggcagcgat tggggcttcg gctaccaact ggcgtggatg tgggacatca
     2701 acgaccgtgc gcgcgtgggc gtgaactacc gttccaaagt ctcacacacg ctcaaaggcg
     2761 atgccgaatg ggcggcagac gacgcaatgg cgaaacagtt atgggatgca aacaaactcg
     2821 cactgctcgg ctacacgcca agcgaaaaag cccgcgttaa aatcgttacg cccgagtctt
     2881 tgtccgtaca cggtatgtac aaagtgtccg acaaagccga cctgttcggc gacgtaactt
     2941 ggacgcgcca cagccgcttc gataaggcgg aactggtttt tgaaaaagaa aaaaccatcg
     3001 tcaacggcaa atccgaccgc accaccatca cccccaactg gcgcaacacc tacaaagtcg
     3061 gcttcggcgg ttcttatcaa atcagcgaac cgctgcaact gcgcgccggc atcgcttttg
     3121 acaaatcgcc cgtccgcaac gccgactacc gcatgaacag cctgcccgac ggcaaccgca
     3181 tctggttctc cgccggcatg aaataccata tcggcaaaaa ccacgtcgtc gatgccgcct
     3241 acacccacat ccacatcaac gacaccagct accgcacggc gaaggcaagc ggcaacgatg
     3301 tggacagcaa aggcgcgtct tccgcacgtt tcaaaaacca cgccgacatc atcggcctgc
     3361 aatacaccta caaattcaaa taaacggccc gccgtttgat tgtagaaatg ccgtctgaaa
     3421 cagatttccg tttcagacgg cattgtttta ggcgcgattt atagtggatt aacaaaaatc
     3481 ag
//
LOCUS       DAWKOD010000180         3409 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000180 DAWKOD010000000
VERSION     DAWKOD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.180"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(236..616)
                     /locus_tag="WJK73_001891"
     CDS             complement(236..616)
                     /locus_tag="WJK73_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ7558325.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMSSVQNQARPADF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1092..3173
                     /locus_tag="WJK73_001892"
     CDS             1092..3173
                     /locus_tag="WJK73_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ7558326.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3163..3357
                     /locus_tag="WJK73_001893"
     CDS             3163..3357
                     /locus_tag="WJK73_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ7558327.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          744 a         1007 c          876 g          782 t
ORIGIN      
        1 acagcttcga caaagacgcg gttgccaaag agatcgacct acaaagggaa gtaacacagg
       61 cgttcggcaa aacgtcgccc aagtcgcagc ggccgttgcc aacaaacttg gcaatacccg
      121 aagttacgga cggtatcagg tagccaaaac cctgccggag gccgaactgc aaaacaaatc
      181 cgacataaaa aaccgcccga aaaatccgga cggcggttca aacaggctgc cccgtttagc
      241 gggcgcggca ggaagtttcg accgaattgc cgtaggcatc ggtaaagccg aaaaaggctt
      301 cgccgccttt ctggtgccac tcggttgcgt ttccgaacaa accgtgttcg gcggtatagc
      361 gttcgccgga tgcggcaacg tcggaagaga ggacggcacg cctgccgtcc agccgcaacg
      421 cgactttgcc gctgtccaaa tggcggacgc gcacagacaa accgttctcg catgaaaacg
      481 cccgaaaatc tgccgggcgg gcttggtttt gaacggacga catatgccct cgtccgccgt
      541 catcatacgc ctccggcacg gcacaggccg ccaaagacaa aaccggtacg gtcagcgcga
      601 aaaacctgat attcataaaa gctccccaat aaaaataaga tatgaaacaa ccgccctgat
      661 tccaagctgc ggcaacgcca tactataaac ggacgcgcaa acacacaagc ccgataaccg
      721 gaatttaccc gcgatgaatc aataatccgg attgcgcgcc ccttctttac cggtcttccg
      781 gcgccgcctt ttgcctgacg atgccgtcca aacccgcctg tccgcccgaa ggaatgtaaa
      841 ttttttccaa attccaagta aaaaccgcta tcagtgtgct aatttgcgtt aaaatcctat
      901 tcggcgttta acgttttgtg cgcccgcatc cctgcactgt ttgatgcggg cataaggcac
      961 aaatcccgac aagcgcactg tttcatactt cgtcaatcat tcagactccg gtttgtgccc
     1021 gtgccggcag gcggtcatcc gtttcccgcc gttcaggcat attccgacag tgtgagataa
     1081 ggatttattc gatgaaatca ctcaaaacct tcctcatttg gggcatagtg gtactggtcg
     1141 gcttagcatc ctttaccact ctggctctca gccgaggcga acaggtcagc gcggtatgga
     1201 tggttaccgc cgccatatcc gtttactgca tcgcctaccg tttttacagc ctctacatcg
     1261 ccaaccgcgt aatgcggctc gatcctaacc gcctgactcc ggcagaacgc cacaacgacg
     1321 gcttggacta cgttccgacg cacaagggcg tattgttcgg acaccacttt gccgcgattg
     1381 ccggcgcggg ccccttggtc ggcccggttt tggcggcgca aatgggttac ctgcccggta
     1441 ctttgtggat tatcttcggc gtggtgtttg ccggcgcggt acaggatatg atggtcttgt
     1501 tcgtctctat gcgccgcgac ggtaagtctt tgggcgatat tgtgaaacag gaactcggca
     1561 ctgtccccgg cgtgattgcc tccatcggta ttttgatgat tatggtcatc attatggcgg
     1621 tgttggcgtt gattgtcgta aaagcattgg ttcacagccc ttggggtacg ttcaccattg
     1681 ccgcaacgat gccgattgcc ctgtttatgg gtatttacac gcgctatatc cgtccgggca
     1741 aaatcggcga gatttccatc gtcggcttta ttttgctgat gctggcggta atttacggcg
     1801 aagatgtggc gaaaagttcc atcgggcatt ggttcgacct tgacggcatc cagctcactt
     1861 gggcgattat gatttacggc tttgtcgcct ccgtattgcc cgtatggttg ctgctcactc
     1921 cgcgcgacta tctctccacc ttcctgaaaa tcggtacgat tgcggccttg gctttgggca
     1981 tcgtcatcgt caatcccgct ttgcaaatgc ctgccgtaac ccactttatc gacggttcgg
     2041 gtccggtatt ctcaggcgca ttgttcccgt tcctcttcat caccatcgcc tgcggtgcgg
     2101 tttcgggctt ccacgcgctg atttcctccg gcaccacgcc gaaaatgctg gaaaacgaaa
     2161 cccacgtccg tatgattggt tacggcggta tgttgatgga aagtttcgta gccattatgg
     2221 cgcttgccgc cgccgcatcg cttgatcccg gcgtgtactt cgccatgaac agccctgccg
     2281 ccctgatcgg tacggatgcc aataccgccg ccgaagtgat taccaccaag ctgcaattcc
     2341 ctgtcgatgc ggcaaccctg ttgcacaccg ctaaggaagt gggcgaaaac accatccttt
     2401 cccgtgcagg cggtgcgccc accctcgcag tcggtatggc gcacattatg agccgcctga
     2461 ttccgggcga ggcgatgatg gcgttctggt atcacttcgc cctgttgttt gaagccttgt
     2521 tcatcctgac cgccgtcgat gccggtacgc gcgtcgcacg ttttatgatt caagacttgg
     2581 gcagcatctt ctacaaacct ttcggcaaca ccgactccat ccccgccaac ctgattgcga
     2641 ccttcttcgc cgtggcattg tggggctact ttctctacac cggcgtaacc gacccgttgg
     2701 gcggcatcaa ctcgctctgg cctttgttcg gcatcgccaa ccaaatgctg gcaggcgtag
     2761 ccttgattat gtgcgccgtg gtgctgatta agatgaaacg cgaccgttat gtctgggtgg
     2821 tactcgttcc cgccgtcggc gtactgttcg taacctgcta cgccggcctg caaaaactgt
     2881 tccacagcga cccgcgcatc agcttccttg cccacgccgg caaatacagc gacgcattgg
     2941 ctaaaaacga aatccttgcg cctgccaaag acatcggcga aatggcgcaa atcatcttca
     3001 acgacaagat taatgccggt ctgaccatcc tcttcttgtc ggttgtcgtg attgtcgccg
     3061 cgtacggttt gcgtaccgcc ctcaaagcac gcaaagtcgg ctggccgacc gccaaagaaa
     3121 tcccggcggt gtaccgcgac ggcaaacagc cggaggcaca aagtgaagca taagctcgcg
     3181 tcttggtgga aaaccatcaa gctgacggca aacttgatgg caggcgtgcc cgattatgaa
     3241 aactacgttg cacagcagcg caaacataat cccaacgccc ctgtgatgac caagctgcag
     3301 tttcaagact attgccgcaa acgccgctgc ggcgcaaacg gcggacgctg ctgttaagcc
     3361 tgcttgaaac aaattccgtc tgaacgccgc ttcagacgga atttttata
//
LOCUS       DAWKOD010000181         3389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000181 DAWKOD010000000
VERSION     DAWKOD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.181"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            36..701
                     /locus_tag="WJK73_001894"
     CDS             36..701
                     /locus_tag="WJK73_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ7558328.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKNGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINNKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYSS
                     DQNLKNNSKFVYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPKNDTRAV
                     CRH"
     gene            733..1344
                     /gene="pilV"
                     /locus_tag="WJK73_001895"
     CDS             733..1344
                     /gene="pilV"
                     /locus_tag="WJK73_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ7558329.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYTPTSSDGDFKLDNL
                     KSKKDLAKAQLDRFGYELKNALPDAAAIHYAVCKDSSGVAPTLSAGSTFSQNCDGSAN
                     GDTLIKVLWVNDSAGDSDIDRTNLETNGNNIVYTYQARVGGRE"
     gene            1341..2282
                     /locus_tag="WJK73_001896"
     CDS             1341..2282
                     /locus_tag="WJK73_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ7558330.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNRL
                     FSLKRNSTNKLIPIAESSNIGYQGFAQARPALIFQYGIDDVNASAETTVVSSCGAISK
                     PGKQIPTLEDAKKELKIQNPDKEQNGNIARQRHVVNAYAVGRIAGEEGLFRFQLDDKG
                     KWGNPQLLAKKIKRMDVRYIYVSSCPEDEDAGKEEQFKYTDKFDSTQNAVTPAGVEVL
                     LDSGLDAKIAASSDNSIYAYRINATIRGGNVCANRTL"
     gene            2261..2860
                     /locus_tag="WJK73_001897"
     CDS             2261..2860
                     /locus_tag="WJK73_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ7558331.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNNGNEEAFDNIVVQGKPTVEAVKRSCPANSTNLCIDNKGMEYKKGTQSVSKPPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2865..3338
                     /locus_tag="WJK73_001898"
     CDS             2865..3338
                     /locus_tag="WJK73_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ7558332.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLGTLSSDVGCEAFSNRKK"
BASE COUNT          957 a          680 c          863 g          889 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcacgcg gagcaggttg
      181 ccaacctttt gcgtttctcc aggggagaag ccgtccggct caatcttcct gtctatatct
      241 gtcctgttca agttaaaaaa ggcggtgcgc ccaacaataa atgtgacttc ggcaagaatg
      301 ggcagggaat gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caataataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggtcaga ctcagccgac cgctgaccgt gtagtttgga
      481 cattcaacca aaacgggaca ttcggctatt cgtccgatca gaatctcaag aataattcca
      541 aatttgttta ttctgacggt tatatccaaa tcgtgttgac agatgcgaag gcggtttctg
      601 ccgatgaaaa gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgaag
      661 tttgtcctaa aaacgatacg cgcgccgtat gccgacatta atgattgttt tagtcggttt
      721 gggaaataaa aaatgaagaa taatgattgc ttgcgcctga aaaatcccca gtccggtatg
      781 gcgctgatag aagtcttggt tgctatgctc gttctgacca tcggtatttt ggcgttactg
      841 tctgtgcagt tgcggacagt cgcttccgtc agggaggcgg agacacaaac catcgtcagc
      901 caaatcacgc aaaacctgat ggaaggaatg ttgatgaatc cgaccattga ttcggacagc
      961 aacaagaaaa actataatct ttacactggg tcgtacaccc ccacttcctc tgacggcgat
     1021 ttcaagcttg ataatttgaa aagcaagaag gatttggcaa aggcccagtt ggacaggttc
     1081 ggttatgaat tgaaaaatgc cttgccggat gcggcagcca tccattacgc cgtctgcaag
     1141 gattcgtcgg gtgttgcgcc gacattgtcc gccggcagta ctttttctca aaattgcgat
     1201 ggtagtgcaa atggggatac tttgattaaa gtattgtggg taaatgattc ggcaggggat
     1261 tcggatatcg accgtacgaa tcttgagacg aacggcaaca atatcgtata tacctatcag
     1321 gcaagggtcg gaggtcggga atgagacgta aaatgctaaa cgtaccaaaa ggcagttatg
     1381 atggtatgaa aggttttacc attattgaat ttttggttgc gggcctgctc agtatgattg
     1441 tcctgatggc ggtcggatcg agttacttca catcccggaa attaaatgat gcggcaaacg
     1501 agcgtcttgc cgcacaacag gatttgcgga atgcggcaac attgattgtc cgcgatgcaa
     1561 gaatggcagg gggcttcggt tgtttcaata tgtccgagca tcctgcaact gatgttgttt
     1621 ccgatgtggc gcaaaaaaac cgtctttttt ccttaaaaag gaacagtaca aataaactta
     1681 tccccatagc ggaatcttca aatatcggat atcagggttt tgcccaggct cgtccggcat
     1741 tgattttcca atacggaatc gatgatgtta atgcaagcgc cgagactacc gtcgtcagca
     1801 gctgtggcgc aatatcgaaa ccgggtaagc aaatccctac tttagaagat gcaaaaaaag
     1861 aattgaagat tcagaatcct gataaggagc aaaatggcaa tatagcgcgt caaaggcatg
     1921 tggtcaatgc ctatgcggtc ggcaggattg ccggtgagga aggtttgttc cgcttccaat
     1981 tggatgataa gggcaagtgg ggtaatcctc agttgctcgc gaaaaagatt aaacgtatgg
     2041 atgtgcggta tatctatgtt tccagttgtc ctgaagatga agatgccggg aaagaggaac
     2101 aattcaaata tacggataaa ttcgacagca cccaaaatgc tgttacgcct gccggggtgg
     2161 aggttttatt ggatagcggc cttgatgcca agattgccgc ttcttcagac aatagtattt
     2221 atgcttaccg tatcaatgcg acaatacgcg ggggaaatgt atgcgcaaac agaacacttt
     2281 gacgggaatc ccgacttctg acggacagag ggggtttgca ctgtttatcg tactgatggt
     2341 gatgatcgtc gtggcatttt tggttgtaac tgccgcgcag tcttacaata ccgagcagcg
     2401 gatcagtgcc aacgaatcag acaggaaatt ggctttgtct ttggccgagg cggctttgcg
     2461 ggagggcgaa tttcaggttt tggatttgga atatgatacg gacagtaagg ttacatttag
     2521 tgaaaactgt gaaaaaggtc tgtgtaccgc agtgaatgtg cggacaaata caaataatgg
     2581 taatgaagag gcttttgaca atatcgtggt gcaaggcaag cccaccgttg aggcggtgaa
     2641 gcgttcttgc cctgcaaatt ctaccaacct gtgcattgac aataaaggga tggaatataa
     2701 gaaaggcacg caaagtgtca gcaaaccacc gcgctatatt atcgaatatt tgggcgtgaa
     2761 gaacggagaa aatgtttatc gggttactgc caaggcttgg ggtaagaatg ccaataccgt
     2821 ggtcgtcctt caatcttatg taagcaataa tgatgagtaa taaaatggaa caaaaagggt
     2881 ttacattgat tgagatgatg atagtcgtcg cgatactcgg catcatcagc gtcattgcca
     2941 taccttctta tcaaagttat attgaaaaag gctatcagtc ccagctttat acggagatgg
     3001 tcggtatcaa caatactgtc aaacagttta ttttgaaaaa tcccctggac gataatcaga
     3061 ccatcaagag caaactggaa atatttgtct caggctataa gatgaatccg aaaattgccg
     3121 aaaaatataa tgtttcggtg cattttgtca ataaggaaaa accaagggca tacagcttgg
     3181 tcggtgttcc aaagacgggg acgggttata ctttgtcggt atggatgaac agcgtgggcg
     3241 acggatacaa atgccgtgat gccgcttctg cccgagctca tttggggacc ttgtcctcag
     3301 atgtcggctg tgaagccttc tctaatcgta aaaaataggg ctgttttgcc aatgccgtct
     3361 gaagattaat tttcagacgg tatttttat
//
LOCUS       DAWKOD010000182         3384 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000182 DAWKOD010000000
VERSION     DAWKOD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.182"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1144
                     /locus_tag="WJK73_001899"
     CDS             <1..1144
                     /locus_tag="WJK73_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ7558333.1"
                     /translation="NIVDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLC
                     VDAAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTY
                     VRMMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEE
                     FFPSEVYVFDSLYAASRRNGEPEKNPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGA
                     IIYAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHH
                     NDSMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPR
                     LWDGQNDPDLPAINLTGSGSRWETEKHIAASSSFAFGGSNCVLIIG"
     gene            1198..1273
                     /locus_tag="WJK73_001900"
     tRNA            1198..1273
                     /locus_tag="WJK73_001900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Ile,seq:cat)
     gene            1348..2106
                     /locus_tag="WJK73_001901"
     CDS             1348..2106
                     /locus_tag="WJK73_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7558334.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            2107..3171
                     /locus_tag="WJK73_001902"
     CDS             2107..3171
                     /locus_tag="WJK73_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7558335.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTAGNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT          891 a          906 c          831 g          756 t
ORIGIN      
        1 aaatatagtg gattggcacg aacgtttccc cgaattggaa gcgcaactgg gtgcgccgat
       61 tgaggattac gcgccgccga aacattggac gcgcaagcag ctcagaagta tggggcgtgt
      121 gtcgtatttg tgcgtcgatg cggcggagca ggctttgacg gatgccggtt tgctcgggga
      181 cgaaagcatt accgacggac ggatgggcgt tgcctgcggc tcttccagcg gcagcaccaa
      241 agacatcggc gatgtgggcg aattgttgct gaccggcacg tcgcgcaact tcagtgccaa
      301 cacctatgtg cgtatgatgc cgcacaccgc cgccgccaat atcggcatct ttttcgggct
      361 gaaaggacgc atcatcccga catcgagcgc gtgttcgtcc ggcagccaag gcataggtta
      421 tgcctacgaa gccatcaaat acggtctgac cgatatgatg ctggcgggcg gaggcgaaga
      481 atttttcccg tccgaagtgt atgttttcga ctcgctttat gccgccagcc gccgcaacgg
      541 cgaaccggaa aaaaacccgc gcccatacga cgcgaaccgc gacgggctgg tcatcggcga
      601 aggcgcgggg attttcgtgc tggaagaatt ggaacacgcc aaacggcgcg gtgcgataat
      661 ttacgccgaa ctcgtcggct acggagccaa cagcgatgcc taccatattt ccacgccccg
      721 ccccgacgcg caaggcgcaa tccttgcctt tcagacggca ttgcaacacg caggtcttgc
      781 gcccgaagac atcggctgga ttaatctgca cggcaccggg acgcaccaca acgacagtat
      841 ggaaagccgt gccgttgcag cggttttcgg caacaatacg ccctgcacgt ccaccaagcc
      901 gcaaaccgga cacacgctgg gcgcggcggg cgcaatcgaa gccgcgttcg cgtggggcat
      961 tgccgaccgg caaagcaatc ccgaagggaa acttccgccc cggctttggg acgggcagaa
     1021 cgaccccgac ctgcccgcca tcaacctgac cggcagcggc agccgctggg aaaccgaaaa
     1081 acacattgcc gccagctcgt cgtttgcctt cggaggaagc aactgcgtct taatcatcgg
     1141 atgaaataag tttgtcaatc ccaccgctat gctatacaat acgcgcctac tcttgacggg
     1201 tctgtagctc aggggttaga gcaggggact cataatccct tggtcgtggg ttcgaacccc
     1261 accggaccca ccaattccca agcccggacg tatgtttggg cttttttgcc gccctgtgaa
     1321 accaaaatgc tttgagaaac cttgataatg aaaaaagtca gcgtattgat tgttgccaaa
     1381 aacgaagcaa accacattcg ggaatgtatt gaaagttgcc gtttcgataa agaagttatc
     1441 gttattgacg accacagcac cgacaatact gccgaaattg ccgagggttt gggtgcaaaa
     1501 gtcttcagac ggcatttgaa cggggatttc ggagcgcaaa aaacatttgc catcgaacag
     1561 gcaggcggag aatgggtttt cctgattgat gcagacgaac gctgcacgcc ggaactatct
     1621 gatgaaatct caaaaattgt ccaaaccggc gattatgccg cctattttgt cgaacgccgc
     1681 aaccttttcc ccaaccatcc cgccacacac ggcgcgatgc gtcccgacag cgtatgccgt
     1741 ctgatgccga aaaaagacag ttcggtgcaa ggcaaagtac acgaaaccgt acaaaccccc
     1801 taccccaaac gccgtctgaa gcattttatg taccattaca catacgacaa ctgggaacaa
     1861 tatttcaaca aattcaacaa atacacttcc atctcagccg agaaataccg agagcaggga
     1921 aagcccgtgc gtttcgttag ggacattatc ctccgtccga tttggggttt tttcaaaatt
     1981 tatatcctga acaaagggtt tcttgatgga aaaatgggtt ggattatgtc cgtcaaccac
     2041 agctattaca caatgattaa atatgtcaaa ctatattatc tgtacaaatc cggcggaaaa
     2101 ttttaaatgg aaaaagaatt caggatatta aatatcgtat cggccaagat ttggggcggg
     2161 ggcgaacaat atgtctatga tgtttcaaaa gcattgggac tgcggggctg cacgatgttt
     2221 accgccgtca ataaaaataa tgagttgatg cacagacgct tttccgaagt ttcttccgtt
     2281 ttcacgacac gccttcacac gctcaacggg ctgttttcgc tctgcgcgct tacccgcttt
     2341 attcggaaaa accgcatttc ccacctgatg atacacaccg gcaaaattgc cgccttatcc
     2401 atacttttga aaaaactgac cggggtgcgc ctgatatttg tcaaacacaa tgtcgtcgcc
     2461 aacaaaaccg atttttacca ccgcctgata cagaaaaaca ccgaccgctt tatttgcgtt
     2521 tcccgtctgg tttacgatgt gcaaaccgcc ggcaatccct ttaaagaaaa ataccggatt
     2581 gttcataacg gtatcgatac cggccgtttc cctccctctc aagaaaaacc cgacagccgt
     2641 ttttttaccg tcgcctacgc cggcaggatc agtccagaaa aaggattgga aaacctgatt
     2701 gaagcctgtg tgatactgca tcggaaatat cctcaaatca ggctcaaatt ggcaggggac
     2761 ggacatccgg attatatgtg ccgcctgaag cgggacgtat ctgcttcagg agcagaacca
     2821 tttgtttctt ttgaagggtt taccgaaaaa cttgcttcgt tttaccgcca aagcgatgtc
     2881 gtggttttgc ccagcctcgt cccggaggca ttcggtttgt cattatgcga ggcgatgtac
     2941 tgccgaacgg cggtgatttc caatactttg ggcgcgcaaa aggaaattat cgaacatcat
     3001 caatcgggga ttctgctgga caggcttact cctgaatctt tggcggacga aatcgaacgc
     3061 ctcgtcttaa accccgaagc aaaaaacgca ctggcaacgg caggacatca atgcgtcgcc
     3121 aaccgtttta ccatcaacca taccgccgac aaattattgg atgcaatata agccgctttc
     3181 agacggcata tgccgtctga aagcctttga tgcaacaaac cgccaaatta tattcgttca
     3241 ttggaaagaa acaccccgaa tccatcctta aaaataagaa aatcccaata tcccccgata
     3301 ttacgcagcc tattggcaaa gttttgcagc gtcttccccg gcttgtgctg ccgcatcaag
     3361 tgctttgtta caatgtatag taga
//
LOCUS       DAWKOD010000183         3364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000183 DAWKOD010000000
VERSION     DAWKOD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.183"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="WJK73_001903"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="WJK73_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ7558336.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="WJK73_001904"
     CDS             1455..2555
                     /locus_tag="WJK73_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7558337.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2576..3193
                     /gene="thiE"
                     /locus_tag="WJK73_001905"
     CDS             2576..3193
                     /gene="thiE"
                     /locus_tag="WJK73_001905"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ7558338.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNAEEVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          677 a         1048 c          938 g          701 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg
      241 ggcaatgcct cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga attgtccgcc gtcatgccgc tttcctggct gccgctggcc
      841 gccgactaca cacgccaagc acgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tatacgctga cgggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctgt cgaccgttac caccactttt ctcgatgcgt actccgccgg cgcgagtgcg
     1081 aacaatattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtacttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggcttgcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtactggtg cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcactgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag
     1801 cggatgacga aatcgtccgt tggcacgccg acgacatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccttgccat tgggaacacg
     1981 aatgtgcccc cgaagacctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaat gcttctgccg aacacggcag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgcctgctgc
     2161 acccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ctacagccgc aaacgccgcc
     2401 tcatcgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgtcag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgtgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg tctgaaagga accccatgac
     2581 cttcccgcca ctcaaatccc cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt
     2641 ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg cgctgcaaga ccctgcacgg
     2701 cgatgaattg aaacgcgaaa tcgcccgctg cgtcgccgcc tgtcaaggca gccgtacgca
     2761 gcttttcatc aacgaccact ggcgcgaggc aatcgaagca ggcgcgtacg gcgtgcatct
     2821 cggacaagaa gacatggaca ccgccgacct tgccgccatc gccgccgccg gtttgcgctt
     2881 gggtttgagt acgcactccg ttgccgaact cgaccgcgcc ctgtccgtac accctagcta
     2941 cgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa atgcccaccg ccccgcaagg
     3001 cttggacaaa ctgcgcgaat atgtgaaaca ggcaggcggc acgcccgtcg tcgccattgg
     3061 cggcatcgat ttgaacaatg ctgaagaggt attggcaacc ggcgtttcct cactcgccgt
     3121 cgtccgcgcc gtgaccgaag cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt
     3181 gtgggatgga taaaaccgaa agaagaaaat tcaattgccg tgtaggcaaa acttagcccg
     3241 ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc atttccgcgc
     3301 aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc
     3361 aaaa
//
LOCUS       DAWKOD010000184         3276 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000184 DAWKOD010000000
VERSION     DAWKOD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.184"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(698..1282)
                     /gene="rnhB"
                     /locus_tag="WJK73_001906"
     CDS             complement(698..1282)
                     /gene="rnhB"
                     /locus_tag="WJK73_001906"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ7558339.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1351..3246)
                     /gene="mnmG"
                     /locus_tag="WJK73_001907"
     CDS             complement(1351..3246)
                     /gene="mnmG"
                     /locus_tag="WJK73_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ7558340.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          584 a          787 c         1002 g          903 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaacggaaac
      541 cggacggatt ggattcccgc ctgcgcggga atgacggatt ttaggttttt ttttgatttt
      601 ctatttttcg cgggaatgac ggtttgggtt ctttctcttt ggagttgcga tgccggaaat
      661 gccgtctgaa ggcttcagac ggcatttttg tgccggttta aaacaaggcc tgctgcgcga
      721 gcaggtttct gacgggggcg aagtcgcggc ggtgttcggg cagcacgccg tatttttcga
      781 gggcttccaa atgctgcttc gtgccgtaac ctttgtgttt gtcgaaaccg tattggggat
      841 ggcgttgcgc cagtgcgtac atttccgcat cgcgggcggt ctttgccaaa acggatgcgg
      901 cggatatttc gatgattttg ctgtcgcctt tgacgacggc ttcggcaggg atgttcaaat
      961 gttcgggaat gcggttgccg tcgatgaata ttttttcggg acgcacagcc aaaccgtcaa
     1021 cggcgcgttt catcgcgagc atggtggcgt gcaggatgtt gaggctggcg atttcttcgg
     1081 gcccggcggc ggcgacgtgc cacgcaaccg cctgattttt tatcatttcg gcaagcgcgt
     1141 cgcgtttttt ctcgctgagt tttttggagt cggtcagtcc gggcaggtcg aatgtttccg
     1201 gaaggatgac ggcggcggca aacacgctgc cgactaaagg tccgcgtcct gcctcgtcca
     1261 cgccggcggt cagtatgtgc atgatgtttc ctgtcgggat ggtgggaatg ccgtctgaac
     1321 ggtgtttcag acggcatcgc gccgatgtgt ttatttcgcg tctttaaatc cgcgcttcaa
     1381 atgcaccatc agcagtgcga cggcggcagg cgttacaccg gaaatgcggc ttgcctgccc
     1441 gacggtttcg ggtttgtgct ggttgagctt ttgctgcact tctgccgaca agcctttgac
     1501 tttgccgtaa tcgatgccgt cgggcagttt taaggtttcg atgtcgcggc ggctgtcgat
     1561 ttcttcgttt tggcggtcga tatagccttg gtatttgact tggatttcga cttgttcgat
     1621 gacttcggcg gagaggtttt cagacggcat cgcgccttcg agcgtcatca gcgcggcgta
     1681 gtcgaggttc gggcggcgta acaaatcgtg caggttggct tctcggctga gtttttgtcc
     1741 gaatacgcgg atttgttcgt cttcggcgag tttttgcggt gtgtaccacg ttgttttcaa
     1801 acgttggatt tcgcgttcga cggcttcgcg tttttcgttg aacatacgcc attgcgcttc
     1861 acccaccaag ccgattttgt agccgtcttc ggtcaggcgc atgtcggcgt tgtcttccct
     1921 gagttgcagg cggtattcgg cgcggctggt gaacattcgg tagggttcgt tcacgccttt
     1981 ggtgatgagg tcgtccacca atacgccgag gtaggcttgt tcgcggcgca gcaggagcgg
     2041 gtcttgtccg cgcacatatt gcacggcgtt cgcgcctgcc aataaacctt gcgcggcggc
     2101 ttcttcgtag ccggtcgtac cgttgatttg cccagcgaaa aacagtccct caatggtttt
     2161 ggtttcgagg ctggctttga ggttgcgcgg gtcgaagtag tcgtattcga tggcgtagcc
     2221 ggggcgcagg atatgggcgt tttccaaacc tttcatactg cggacgagcg cgatttggat
     2281 gtcgaacggc aggctggtgg agataccgtt aggatagtat tcgtacgtag tcaggccttc
     2341 gggttctagg aaaatctggt ggctgtcttt atcggcgaaa cggttgattt tgtcttcgat
     2401 agacggacaa taacgcgggc ccacgccttc gattttgccg gtaaacatcg ggctgcggtc
     2461 gaagcctgag cggatgatgt cgtgggtttg cgtgttggta tgcgtaatcc agcaggacac
     2521 ttggcgcggg tgcatttcgg cgttgccgcg cacggacatg acgggaacgg gtgtgtcgcc
     2581 gggctgttcg gtcagttggg aaaagtcaat cgtgcgtccg tcgatgcgcg gcggcgtgcc
     2641 ggttttcaga cggccttgcg gcagcttcaa ttcgcgcaaa cgtccgccca gcgatttggc
     2701 ggcggggtcg ccggcgcggc cgccttcgta gttttccaaa ccgatatgga ttttgccgga
     2761 caaaaatgtg cctgcggtca gcaccacggc gcgggctttg aattccacgc ccatcgcggt
     2821 gattacgccg ctgatgcgtt cgccgtcgag cgttacgtct tcgacggctt gttggaaaag
     2881 gtcgaggttt tcttggtttt ccaacatttc gcggatggcg gctttgtaca aaatgcggtc
     2941 ggcctgcgcg cgcgtggctc gaactgccgc gcctttgctg gcgttcaggc ggcggaactg
     3001 gataccggat ttgtcggtgg ccaacgccat tgcgccgccg agcgcgtcga gttcgcgtac
     3061 caaatgccct ttgccgatgc cgccgatgga agggttgcat gacatttgtc cgagcgtttc
     3121 gatattgtgt gtgagcaaaa gcgtctgcgc gcccatgcgg gcggcggcga gcgcggcttc
     3181 ggtaccggca tggccgccac cgacgacaat cacgtcgtag gttttggggt aaatcatgtg
     3241 ggtcatgtgt gtattgcctg acggtgtttc agacgg
//
LOCUS       DAWKOD010000185         3258 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000185 DAWKOD010000000
VERSION     DAWKOD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.185"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WJK73_001908"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WJK73_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ7558341.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     KQMVLSVKEGDTLEQRDIISTLVSMQYERGEMDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKSRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="WJK73_001909"
     CDS             2186..3070
                     /locus_tag="WJK73_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558342.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
BASE COUNT         1004 a          840 c          682 g          732 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt catccgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcccaccgcc attgcaggcc tgctcgaagg gctttcagac ggccttgcct
      241 atcaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacgctggcc gcccagcttt
      361 acgccgaaat gcgcgaattt tttcccgaaa atgcggtgga atatttcgtg tcttactatg
      421 attattacca gcccgaagcc tatgtgccga gccgtgactt attcattgaa aaagattcgg
      481 cgattaacga acacatcgag caaatgcggt tgagtgccac caaaaacctg atgacgcgca
      541 acgacgtgat tatcgtcgcc accgtgtccg cgatttacgg tatcggcgac ccgactgaat
      601 acaagcaaat ggtgttgtca gtgaaagaag gcgacacact cgagcagcgc gacatcattt
      661 ctacgctggt atcgatgcag tatgaacgcg gcgaaatgga tttcaaacgc ggctcattcc
      721 gtgtgcgcgg cgatgtgatt gacgtgtatc cggcggaaag ctcagaaaac gccttgcgta
      781 tcagcctgtt cgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttatccaacg cgtcggccgc tacaccgtct tcccgtccag ccattacgtt accccgcgcg
      901 ataccgtctt gcgcgcctgc gagtccatca aagaagaatt acgtgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgaacaac aacgcatcga acagcgcacc cgctttgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggcatcga aaattactcc cggcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgcct gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacccaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgact acggcttccg cctgccttcc gcccgcgaca
     1261 accgcccgct caaattccat gaatttgaaa aagtcatgcc gcaaactgtc ttcgtttccg
     1321 ccactccagc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgacccc caaatcatca tccgccccgt cgccacccaa gtcgacgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgttctcgtt accaccctca
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacattgat accgtagagc gcgttgaaat cattagagat ttacggctcg
     1621 gcctgtttga cgtactcgtc ggcatcaacc tcttgcgcga aggcctcgat atccccgaag
     1681 tctccctcgt cgccatcctc gacgccgaca aagaaggctt cctgcgctca caccgcagcc
     1741 tgattcaaac cataggccgc gccgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcagca gattaaaaaa caagtcaaag
     1921 acattatcga cggcgtgtac cacgaagaag acagcggcaa aagccgtctg aaaggtaaaa
     1981 acaaggttaa agtaggcgaa atccacaacg aagaagatgc gattaaagaa atcgccaaac
     2041 tagaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggcatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccacac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcctaaaatg tcggattc
//
LOCUS       DAWKOD010000186         3231 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000186 DAWKOD010000000
VERSION     DAWKOD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.186"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(488..877)
                     /locus_tag="WJK73_001910"
     CDS             complement(488..877)
                     /locus_tag="WJK73_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ7558343.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(1449..1574)
                     /locus_tag="WJK73_001911"
                     /pseudo
     CDS             complement(1449..1574)
                     /locus_tag="WJK73_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1620..2753)
                     /gene="carA"
                     /locus_tag="WJK73_001912"
     CDS             complement(1620..2753)
                     /gene="carA"
                     /locus_tag="WJK73_001912"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ7558344.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          680 a          829 c          895 g          827 t
ORIGIN      
        1 tcaaccaccg aaatacaagc cttgtggctt gttgccctct ctctagctct ctcccacggg
       61 agagaggacg gagtggctgt agggctaagg gttctgcaag gaaacaggtt gtgcaggttg
      121 ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc aagtcgcttt tcaaaccgcc
      181 tgcaaccgca aaatccgctg caacctaccc cctcccccgt gggggagggc tggggagagg
      241 gcattctccg aatttcggca acctttccta acactctaac cgccaagaca caagccttgc
      301 ggcttgttgc cctctctcta gctctctccc acgggagaga ggatggggtg gctgttgggg
      361 ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc agacggcctt
      421 tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa tcaaaatcag
      481 ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg atttctgtca
      541 gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg gtaaagccca
      601 agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg tgctgccctc
      661 cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata tagtttccca
      721 tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg tgctgccaca
      781 attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg cgcaagatgg
      841 ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt gttgtcgtgc tgtttgctgc
      901 cctctctcta actctctccc atgggagaga ggacgtggtg gcggttggga ttaagggttt
      961 tgcaaggaaa acaggttgtg caggctgctt tttatttttc agatgacctg caatcaacgg
     1021 ttgtaaatcc aagttgcttt tcaaaccgcc tgtaaccgca aaacttgctg caacctaccc
     1081 cctcccccgt gggggagggc tggggagagg gcattctccg aatcgcggca atctttccct
     1141 aattccctaa ctgcctcaat acaagccttt cggcttgttg ccctctctct aactctctcc
     1201 cacgggagag aggacggtgc ggctgtagtg attaagggtt ttgcaaggaa aacaaggaaa
     1261 acaagttgta caggctgttt tttatttttc aagcgacctg aaatcaacgc ttgtaaatcc
     1321 aagtcgcttt tcaaactgcc tgcaaccgca aaatctgctg caacctaccc cctcccccgt
     1381 gggggagggc tggggagagg gcattttcca agttgcggca atctttccca aatcccttag
     1441 tcttctaaaa tacaagcctt acggttgttg ccaaatgaaa cttcttccat acccaaaact
     1501 tgcgtgtaaa acgcaatggt tcggtcaatg tcggcaacgg tcagtatcag atggtcgagt
     1561 gcgctaatct tcatttcccg ctttcttttt caggcggcgg cagtgccgtc tgaaagccat
     1621 tatgcccgtt ttgccgcttt catattgcca atgaatttgt caaacaaata gccgacatct
     1681 tgcggaccgg ggctggcttc ggggtggcct tggaagcaga acacaggttt gtcggtcagc
     1741 tcgatgcctt gcaaagtgtt gtcaaacaag gatttgtggg taatgcgtgc gttagcgggc
     1801 agggtgtcgg catcaacggc aaaaccgtgg ttttggctgg tgatgacgac tttgccgctg
     1861 tccaaatctt gcacagggtg gttcgcaccg tggtggctga agcgcatttt cagggttttc
     1921 gcgccgatag cgaggctgat gagctggtgt cccaagcaga tgccgaaaat cggtttgccg
     1981 ctttccatca gtttttgcac ggcttcgatg gcgtaggtgc aaggctcggg gtcgccgggg
     2041 ccgttggaca ggaacacgcc gtcgggattg agtgccaaca cgtcttgtgc gctcgtttgg
     2101 gcggggacga cggtcaggcg gcagccgcgc gaggcgagca tacgcaggat gttggttttc
     2161 acgccgaaat cgtaggcgac gacgtggtaa ggctgttcgt caggggtaac gaaacccttg
     2221 cccaattccc attcgccttc cgtccattcg taagtttccg tgcaggaaac ttcttttgcc
     2281 aagtctttgc cgaccatact gccgaacgcg gcgatgagtt cttgcgcttt ttcgattgtg
     2341 gcatccgcac cggtcagaat cgcgccgcct tgcgcgcctt tttcgcgcaa cagcgtggtc
     2401 aggcggcggg tatcgatgtc ggcgatggcg acggttttgt tgcgtaccaa atagtcgtgc
     2461 aggctttcgg aggcgcggaa gttgctgtgc aagagcggca ggtcgcggat aatcaggccg
     2521 gcggcataaa cgctgcggct ttcttcatct tcggcgttgg tgccggtgtt gccgatgtgg
     2581 gggtaggtga gggtaacgat ttgtttgcag taggacgggt cggtcaggat ttcctgatag
     2641 ccggtcatcg aagtattgaa cacgacttcg ccggaagtcg aaccttcgta accgattgat
     2701 gtgccgtgga atacgctgcc gtcagcgagg acgaggaggg cgggggtgct catgatggga
     2761 atcctgtttt taataaaatt cttgacaatg ccgtctgaag ggacttcggc agggggcatc
     2821 cggtcggaaa aatccggtca aaaaaaaaca cgccgcgcaa gatagacgcg taacgtgctt
     2881 ttcgggcaag tgcctatacc ctgaaagcag catattttaa gacgaaacat acccgatttc
     2941 aagcgcaaac gcggcaggcg ggctgatatg ttcgcgcgaa aacggaaatc cgtggcgtaa
     3001 aaccgcccgc tccgcgccca ctatgcccgt tacgcatttc cccacgtccg tccggcgaaa
     3061 ctatgggaat acccgaatcg tcaggaatac ccgaaccgtc attcccgcgc aggcgggaat
     3121 ccagacctgt cggcacagaa acttatcggg tacaacggtt tcttgagatt ttacgtccta
     3181 gattcccgcc tgcgcgggaa tgacggtatc ggtttgtcgg cgtttcattt g
//
LOCUS       DAWKOD010000187         3192 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000187 DAWKOD010000000
VERSION     DAWKOD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.187"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(159..2291)
                     /locus_tag="WJK73_001913"
     CDS             complement(159..2291)
                     /locus_tag="WJK73_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ7558345.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3073..3192)
                     /locus_tag="WJK73_001914"
                     /pseudo
     CDS             complement(3073..3192)
                     /locus_tag="WJK73_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          919 a          864 c          710 g          699 t
ORIGIN      
        1 gatgagtgaa aaagaagtgc aggctgccta aaaagacaga aaaagtcttt caggcagcct
       61 gcactttggt ttcatttcag tcagtaaacc cagtaaacga cggtctgaaa acgcagaacg
      121 ttacgaaaaa agcagcctac acgcccatcc cccgccttct acccgttctg taaatcatac
      181 agatagcggt aatatccgtt cggcttcgcc agcaattcct gctgtgttcc cgcttccaca
      241 atcctgcctt tatccatggc aatgatccgg tgtgccgttt taacggtgga cagacggtgg
      301 gcgataatca gcaccgtccg gttggcgcaa atggcctgca tgttctgcat aatcgctcgt
      361 tcactttcat aatccagcgc gctggtggct tcgtcaaaaa tcagaatgcg cggattggtg
      421 attaacgcgc gggcaatcgc aatacgctgc cgctgtccgc ccgacaagcc ggccccttgt
      481 tcgcccacca cggtgccgta gccttccggc agctccataa taaactcgtg tgcgcccgcc
      541 agtttggctg cttcgataat gcgttccagc ggcatacccg tatccgtcag cgcgatattg
      601 tcgcgtatgc tgcggttgag cagcacattc tcctgcaaga ccacgccgac ctgccgccgc
      661 agccaggcag gagcggccaa agccaaatcg ttgccgtcca ccaacacccg tccctgctcc
      721 ggtacataca gacgctgcac caatttggtg agtgtggatt tgcccgaccc cgaacgtccc
      781 acaatcccca gcacttcccc cgcccgaatc cgcaggttca aatcctgcaa aatcagcctg
      841 ccgtccgcct tatagcggaa atcgacatgt tcgaacgtaa tctccccccg gatatcgggc
      901 aaagccaaat gcgaagacgc attctcggtc ggcgcattca gaatatcccc caaacgcgcc
      961 accgaaatcc ccacctgctg gaaatcctgc cacaactgcg ccaaacggat aacaggcgcc
     1021 gccacctgtc ccgagagcat attaaacgca atcagctgcc ccaccgtcag cttgccctca
     1081 attaccagcc gtgcgccaat ccacaacgtc gccaccgtca ccagcttctg aatcagctgc
     1141 accccctgct ggccgaccac cgccaacttc gttacccgaa atcccgaagc cacataagcc
     1201 gccaactgat tgtcccaacg ctgcgtcatc tgcggctcca ccgccatcgc ctttaccgta
     1261 cccaccgcag tgatgctttc tactaaaaac gactggttgt ctgcattgcg cgcgaactta
     1321 tcgttcagac gcgtccgcag tatcggactg ataaatgccg accaaaacgc ataggcaggc
     1381 aacgaagcca ataccaccca agtcagagtg gagctgtaat accacatcac cgccagaaag
     1441 ataaacgaaa acgccaaatc caacaccgaa gtcagcgcct gaccggtcaa gaaattgcga
     1501 atctgctcca attcccgcac ccgagccacc gtatcaccca ctcgtctgtg ctcgaaatag
     1561 gataaaggca gggaaagcag atgccggaac aaacgcgcgc ccaattccac atcaatacgt
     1621 gaagtcgtat gtgcaaacag atacgtccgc aaaccgccca acacaatctc aaacagcgac
     1681 accaccaaca aagccaccga caccacatcc aaagtagaga atccccgatg taccagcacc
     1741 ttgtccatca ccacttggaa aaacagaggc gtaatcagcg caaacagctg caacaccacc
     1801 gacaccacca atacttcaaa aaacaaccgg cggtatttga ttaccgccgg aataaaccag
     1861 gtaaagtcaa actttgccaa actgcccaat accgaagcgc gggaagcaac caatatcagt
     1921 ttgcccgaat atctgttaga aaattcggca aaagacaata ccgcagactt attcgtagtt
     1981 aaatcctgta ttagatattg ggcatgctca cccccaccgt ctgttttagc caaaataaaa
     2041 tggttgccgt catcacacca taccaatgcg ggtaaagtcg ccatagccaa acgtttaata
     2101 ggctggcgga ctacctttgc cttcaatccc aaagatttgg cggctaacag ccattgcgtt
     2161 tcatttaaat cgctctgtgc ggaagtacaa aattcatgct gtatatcggc aggattggcg
     2221 gcaatgccgt ggtaatgggc gaggatgatg agggcggaaa gggcggggag cggtgcggat
     2281 acgatagaca taaataaaat atagttagat tggatgtgga taacggctgg ctggaaaagg
     2341 aatatattaa gtagaaagaa atatataaat aaaacagcag aacgcattgt aaggatatat
     2401 atgggaattg taaagagaaa gtatggaaaa gttctcgttt caggaaggta aaacggctta
     2461 ggaatcgagt tagatgagga tgcctcgcac ctctcgtgcc tcctgcatac cgttaaggaa
     2521 cagggttaag gtgcaggctg ctccgaactc tgttgcggtc ggataatgtt attttttgtg
     2581 tttcaggcag cctgaaatat ctgtatattt ttgttttaaa tagattttaa agattgataa
     2641 ctgttcttga cgatttttca agaaaggagt aaagtgactt attatcaatg acaagcaacg
     2701 cgcgaagtga caaggaaaac tatctactta aattctaagg aggcttcgaa tatcataaac
     2761 caatcagaaa catagagata aaaattatgt acaaatataa tcctcttata caatttattg
     2821 cacagttgat tatgtcttat ggagcaagcg tagggtgggc acttgctgcc cacgcgtttc
     2881 atatttcaag gcagcctgaa actgtgtggg cataatgcct accctacatc ccaaaaaaac
     2941 aagcgcagcc tgcgtgtgta gggtgcgaac tttcggcagg cagacacgcg gttttatagt
     3001 ttcaagctga gttgggatgc ttaagataaa gtacaaaacc ttaaaaaata agggggctgt
     3061 actagattag ccctaaattc cacaccaatc ccgcaaaatt tttagctgtc gggacggtgt
     3121 gccgaagtta aatcgaaatt cgcattcttt caagaacagc gggaaagatt tgcgatcaat
     3181 tccgttctat tt
//
LOCUS       DAWKOD010000188         3191 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000188 DAWKOD010000000
VERSION     DAWKOD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.188"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            152..2917
                     /locus_tag="WJK73_001915"
     CDS             152..2917
                     /locus_tag="WJK73_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7558346.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            2960..>3191
                     /locus_tag="WJK73_001916"
     CDS             2960..>3191
                     /locus_tag="WJK73_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558347.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFI"
BASE COUNT          907 a          790 c          764 g          730 t
ORIGIN      
        1 acaacgctgt accggttttt gttaatccac tataaatatc caattgaaat cttcagacgg
       61 tatatcgaat ttacactttt taatgtttat gccgcctaaa aaaatgctaa tatatttctt
      121 aattgtctga ctgtttattg ttgaggaaaa tatgagatct tctttccggt tgaagccgat
      181 ttgtttttat cttatgggtg ttatgctata tcatcatagt tatgccgaag atgcagggcg
      241 cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg cacgtcaagg cgaagcgcgt
      301 accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta tcgacccgtc aggatatatt
      361 caaatccagc gaaaacctcg acaacatcgt acgcagcata cccggtgcgt ttacacagca
      421 agataaaagc tcgggcattg tgtctttgaa tattcgcggc gacagcgggt tcgggcgggt
      481 caatacgatg gtggacggca tcacgcagac cttttattcg acttctaccg atgcgggcag
      541 ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc aattttattg ccggactgga
      601 tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac agccttgccg gttcggcgaa
      661 tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat aatacctacg gcctgctgct
      721 aaaaggtctg accggcacca attcaaccaa aggtaatgcg atggcggcga taggtgcgcg
      781 caaatggctg gaaagcggag catctgtcgg tgtgctttac gggcacagca ggcgcagcgt
      841 ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc ggaaattttg gcgcggaata
      901 tctggaacgg cgcaagcagc aatattttgt acaaacaggc gggttgaaat tcaattccaa
      961 cagcggaaaa tgggaacgcg atttgcaaag gcaattctgg aaaaccagtt actataaaca
     1021 atacaaagac ccccagaaac tgaaaaaata catcgaagac caagataaag actggcggga
     1081 aaacctggcg ccgcaatacg acatcacccc catcgatccg tccagcctga agcagcagtc
     1141 ggcaggcaat ctgtttaaat tggaatacga cggcgtattc aataaataca cggcgcaatt
     1201 tcgcgattta aacaccaaaa tcggcagccg caaaatcatc aaccgcaatt atcaattcaa
     1261 ttacggtttg tctttgaact cgtataccaa cctcaatctg accgcagcct acaattcggg
     1321 caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg gggcttttaa aggattttga
     1381 aacctacaac aacgcaaaaa tcctcgacct caacaacacc gccaccttcc ggctgccccg
     1441 cgaaaccgag ttgcaaacca ctttgggctt caattatttc cacaacgaat acggcaaaaa
     1501 ccgctttcct gaagaattgg ggctgttttt cgacggtcct gatcaggaca acgggcttta
     1561 ttcctatttg gggcggttta agggcgataa agggctgctg ccccaaaaat caaccattgt
     1621 ccaaccggcc ggcagccaat atttcaacac gttctacttc gatgccgcgc tcaaaaaaga
     1681 catttaccgc ttaaactaca gcaccaatac cgtcggctac cgtttcggcg gcgaatatac
     1741 gggctattac ggctcggatg acgaatttaa gcgggcattc ggagaaaact cgccgacata
     1801 cagggaacat tgcaaccaga gctgcggaat ttatgaaccc gtattgaaaa aatacggcaa
     1861 aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac ttcggcgatt atttcatgcc
     1921 gttcgccagc tattcgcgca cacaccgtat gcccaacatc caagaaatgt atttttccca
     1981 aatcggcgac tccggcgttc acaccgcctt aaaaccagag cgcgcaaaca cttggcaatt
     2041 tggcttcaat acctataaaa aaggattgtt aaaacaagat gatacattag gattaaaact
     2101 ggtcggctac cgcagccgca tcgacaacta catccacaac gtttacggga aatggtggga
     2161 tttgaacggg gatattccga gctgggtcag cagcaccggg cttgcctaca ccatccaaca
     2221 tcgcaatttc aaagacaaag tgcacaaaca cggttttgag ttggagctga attacgatta
     2281 tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa agcacgcaac cgaccaactt
     2341 cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa gaccaactca aacaaggtta
     2401 tgggttgagc agggtttccg ccctgccgcg agattacgga cgtttggaag tcggtacgcg
     2461 ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc tatttcggca agagcatccg
     2521 cgcgacggct gaagaacgct atatcgacgg caccaacggg ggaaatacca gcaatttccg
     2581 gcaactgggc aagcgttcca tcaaacaaac cgaaactctt gcccgccagc ctttgatttt
     2641 tgatttttac gccgcttacg agccgaagaa aaaccttatt ttccgcgccg aagtcaaaaa
     2701 tctgttcgac aggcgttata tcgatccgct cgatgcgggc aatgatgcgg caacgcagcg
     2761 ttattacagc tcgttcgacc cgaaagacaa ggacgaagac gtaacgtgta atgctgatgg
     2821 cttgtgcaac ggcaaatacg gcggcacaag caaaagcgta ttgaccaatt ttgcacgcgg
     2881 acgcaccttt ttgatgacga tgagctacaa gttttaaagg cagcccgcgt tttgtggaaa
     2941 accgcaaaaa aaagccaaaa tgccgtctga aagcccttca gacggcattt gtttccccaa
     3001 acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca actcaaagaa
     3061 aaatccattt cattcccacg cagggaagcc ggtttttgat ttcggttatt tttggttgtt
     3121 tcgggtaatt tatgagtcgt cattcccgca aaagcgggaa tctagacatt caatgctaag
     3181 gcaatttatc g
//
LOCUS       DAWKOD010000189         3160 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000189 DAWKOD010000000
VERSION     DAWKOD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.189"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1161)
                     /locus_tag="WJK73_001917"
     CDS             complement(<1..1161)
                     /locus_tag="WJK73_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7558348.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDSVVSLSAHFADGLHTLQADDDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAAAAARQSVLPLMILLTVWITHKAMRPVRKLSQSLEQRRINGLSALSE
                     DNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAERLNNMPL
                     PPEAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQVLQELMP
                     LALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFTDEGKYL
                     AVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHLE"
     gene            complement(1157..1332)
                     /locus_tag="WJK73_001918"
                     /pseudo
     CDS             complement(1157..1332)
                     /locus_tag="WJK73_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1551..2864)
                     /locus_tag="WJK73_001919"
     CDS             complement(1551..2864)
                     /locus_tag="WJK73_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ7558349.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT          807 a          988 c          740 g          625 t
ORIGIN      
        1 ttccaaatgt ccgccgtatt ttttggcaag cgtgtcggca atcgacagcc ccagccccgt
       61 cccctgctgc tccgttccca aaatacggta aaacggatcg aggacgcggg cgcgttcgga
      121 ttcgggaatg ccgttcccgt tatcttccac ccacacggcg agatatttcc cttcgtccgt
      181 gaaacccaaa tctatcctgc cttcgggcgg cgtgtaacgt accgcgttgt cggcaaaggt
      241 tttaatcagc gtatagattt ccgtttcgtc ggcagacact tcgacatcgc ctccgaccgc
      301 cacgccgatg tcctgacgtt tttccaaagc cagcggcatc agttcctgca acacttggcg
      361 gaaacggctt tgcagaccga atgtcgtttt cgtcagaggg gtttcatccg actgcgaacg
      421 cgccagtgcc aaaagctgtt cgagcaggtg tttgttgcgc ctgatgctct gctgcaaaac
      481 ggcagactgc cgcgccgctt cgggcgggag cggcatattg ttgagccgtt ccgcctgaag
      541 ggaaagggct gtcatcggcg tacgcaattc gtgtgccgcg tcggcgacaa accgctgacg
      601 gtggcggatg tcttcatccg cacgtttcaa aagcaggttg atggcggtta cgaaccctct
      661 gatttcactg ggaatattgt cctcactcaa ggcagacagg ccattgattc ggcgttgttc
      721 gagactttgc gacaatttgc ggacggggcg catggctttg tgcgtaatcc acacggtcag
      781 cagtatcatc agcggcaaca cgctttgccg tgccgctgcc gccgccaaat cttcacggta
      841 ttcattttcc tgcatgacgg caatccgtcc ctgttcagtc gtgcggatat agacgcggta
      901 ataatcgtcg tcatcgtccg cctgaagcgt gtgcagacca tccgcaaaat gcgcggacag
      961 gctgacaaca gagtcttcct gctgcggcat ctgcacgaaa atgcgcgtat cgccgtcgcc
     1021 ctcgggcaaa gtttcgggtt tagaatcggg tgcgacgtac aatgccgcct gacggagcag
     1081 gtcgtcctgc aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag
     1141 cattgccagc ggaacaaaca tccaaaccgt cccgccccgg caaacccaaa tccagcagca
     1201 tcaagtcata aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata
     1261 gccgccgtct ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag
     1321 caaaacacgc atcaactttc ccttcaaaat aaaccgtgcc tattctaaca ccccaaaatt
     1381 agccgcaatt tagcggcctt tacgcttgcc ggtatttttc aaaactgcgg cacaaaaaaa
     1441 ccgcgccggc aactgccttc agacggcatt ggggcgcgat tgcaacacac gggcaggcag
     1501 agcctgcgac agaccacagg aacgattcag gcttcagacg gcttcgccgt ttacggcaga
     1561 ggcacgattc ctgccgctat cgaactggcc aatatcgcca gcgacaaacc ccacgcccag
     1621 aaaaacgaat aacggatgtg tttgcccatc gacaatttcg ccaaacccaa gcccatccac
     1681 aaggccggcg aaagcggcgt aacaaaagtg ccgacgatac tgccgatcaa catcgcataa
     1741 cctgctgctt cgggcgccac gcccgcctgc gaggtaatct gctccacaat cggaaacagt
     1801 ccgaaataat aagcgtccgt actcaaaacc aactcaagcg gaatgcccaa cacaccgatg
     1861 gcaatatgca gataaggcag cagcgcgtcc ggcaggatat gcacaatgtc tttggaaatc
     1921 gcgtccaaca tccccgcacc cttcaaaatc cccaaaaacg tacctgccgc caaaataatg
     1981 gacgccatca tcaccgcgcc gccggcgtgg gcataaatcc gctccatctg ttcctgcggg
     2041 ctgcggtaat tcaaaagcaa cgccgccgtt gcagccagca taaatacata acccggcggg
     2101 aagatgcccg aaaaaagcag gctcatcgcc gccaaaaaca gcaggacatt ccaccaaaac
     2161 agtttcggac gcgccaattt ttgttcttct tccgacaaag gcaccggctt tatcaaatcc
     2221 gccacggcgg gcaacgcgcc caactcccgg acaatccgcc ttttttcacg cacacccaaa
     2281 agcagggaca gcgcaaggat aaacactaca ccgataattt gcaccgtcaa caaaggttta
     2341 tacaattcgc ccacatctgc gcccaacacg cttgcaaccc gcccagtcgg cccgccccac
     2401 ggcagaaggt taatcaatcc cgcactggaa gttagcagca aaaacagcag gtaaggattc
     2461 atatgcagac gcttgtaaag cggcaaaagg gcggggacga ccaataaaaa cgtcgtcgca
     2521 cccgccccgt ccaactgcgc caccaccgac accaagaccg tccccacact cactgccacg
     2581 atattacccc gagtcagctt aatcaaaccg cctatcatcg gacgaaacag ccccacatcg
     2641 ttcatgattc caaaaaacaa aatggaaaac ataaacataa tcacaatctg catcaccgat
     2701 ttggtgccgc ccgaataaaa ttcttttaat tgggatacat caaaccccgc cagcaacgcc
     2761 ccaaacagcg gcaccaagat taatgcgatg atgggcgaca ctttttccgt cagcagcaac
     2821 catacgatga ccccgataat cagcagtccg ataaacgtca gcatcatttc tcctttattt
     2881 tattttaaac agaaaaccga ccgtgcaggc aaaaccgccc acagacgcgg gataagccct
     2941 gcattctact tttttatttt gaaacaagtc aatcggtcat ttcctcccat tcacgcctgc
     3001 cgccattcct gcatccgtcc gtcatttcac ggcggcaacc gatacggaac aaccggcaaa
     3061 tcggtatcgg gacggcgcgg gggcattcat cccggtgcgc cgattcaaac gaaaccgccc
     3121 ctatcattgc ggagcgcggg gcgtgccata cacgcgggat
//
LOCUS       DAWKOD010000190         3076 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000190 DAWKOD010000000
VERSION     DAWKOD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.190"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            133..711
                     /locus_tag="WJK73_001920"
     CDS             133..711
                     /locus_tag="WJK73_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7558350.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            816..1484
                     /gene="hda"
                     /locus_tag="WJK73_001921"
     CDS             816..1484
                     /gene="hda"
                     /locus_tag="WJK73_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ7558351.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            1481..2149
                     /locus_tag="WJK73_001922"
     CDS             1481..2149
                     /locus_tag="WJK73_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7558352.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT          870 a          861 c          608 g          737 t
ORIGIN      
        1 gtgaaaaagc tggcgtaact tttccggata caaaaaaacg ccgaaatcca cgtcttccga
       61 agatttcagc gtttttttac tctatttgtt ccttgtgcaa aaactaacag tcccccacag
      121 gaaaacgtcg ccctgttccg ccacaacggc gaaatcctgt tgagcggcat caacatcaaa
      181 ctcaaaagcg gcgattccct gctgatacgc ggcccgagcg gttgcggcaa aacttcgttg
      241 ctgcgcgcgc tggctgggct ttggccgttc ggcagcagcg gcaaagtcag ccgtccgccg
      301 catcaagaca tcctcttcct gccgcaacgc ccgtacacgg cacagggcag cctgcgcgac
      361 gcggtttgtt atcccgacat cgacaaacag catccggagc taatcgaagc catgaacacc
      421 tgccgcttgg gttatctgat tgacaaattg gacaaaacag acgactggca gcacaaactc
      481 tccctgggcg aactgcaacg cgtcgccttc gtccgcgccc tgctttccaa gcccaaaatc
      541 gtcctgctcg acgaagccac cgccgccttg gacgaaccgg ccgaagccgc gctctaccgc
      601 gccttaaagc aaaaactgcc cgacagcatc atcatcagca tcggacaccg aagcacgctc
      661 aacgcgttcc acaatatgcg cctcgatgtc ggcaacgttg cctgcggttg aacgatgccg
      721 tctgaagccg ttttcagacg gcatcgttca aattgataaa gcacgcgaaa acaacgtaca
      781 ataaacccgt ttaccacccc tcggaatgcc gaaccgtgaa ccagcttatt ttcgactttg
      841 ccgcacacga ctatccgagt ttcgacaaat tcctcggcac ggaaaacgcg gaactggtct
      901 atgtcctccg acacaaacac ggacagttta tctatgtctg gggtgaagaa ggtgcgggca
      961 aaagccatct tttgcaggcg tgggtcgcac aggcgctcga agccggcaaa aacgccgcct
     1021 atatcgatgc cgcctccatg ccgctgaccg atgccgcgtt cgaggcggaa tacctcgccg
     1081 tcgatcaagt cgaaaaactg ggcaacgaag aacaagccct gcttttttcc atcttcaacc
     1141 gcttccgcaa cagcggcaaa ggctttttgc tgctcggttc ggaatacacg ccccagcagc
     1201 tcgtcatccg cgaagatttg cggacgcgta tggcgtactg cctcgtttac gaagtcaagc
     1261 ccttaaccga ccaagaaaaa atcgacgcgc tcgccagtat ggcggcggca cgccaagtaa
     1321 ccgtcgattc cgaaattttc gaatacctgc tcaaacactg gcggcgcgat atggacagcc
     1381 tgatgatgat gctcgacacg ctggacaact acgccgtcac aatgggcaaa cgcatcaccc
     1441 tgccgctttt gcgccagctt ttgaaacaac aggaaaccca atgaaaaacc tcgccatctt
     1501 cgacctcgac aacaccctca tcaacaccga ttccgaccac gcctggccgc aatacctcat
     1561 caaaaaaggc ttggttgacg ctgccgaaac cgaagcgcaa aacgaaaaat tctaccgcga
     1621 ctaccaaaac ggttgcctcg acatcgacgc attcctcaaa ttccaccttg ccccgctcgc
     1681 tcgttacagc aaagaagagc tggcagagtt tcaccgcgaa tttatggcgg aatacatcat
     1741 cccccacatc tcgcctatgc agcgtatgct ggtgcagagc caccaaatgg caggcgacga
     1801 aacccttgtg atttcctcaa ccaacgagtt catcatcacc cccgtctgcc accttttcgg
     1861 catcaccaac atcatcggca cacaactcga aaccggttct gacggccgct acaccggcaa
     1921 ttacatcggc acgcccagcc tcaaagaagg caaaatcacc cgcctgaacc aatggctcgc
     1981 cgaacgcggc gaaacgctcg aaagctacgg caaaacctat ttttacagcg actccaaaaa
     2041 cgacctgccg ctgctgcgcc tcgtcgacga acccgtcgcc gtcaaccccg atgccgagct
     2101 ggaaaaagaa gccaaagaaa aaggctggcc ggttttgaat ttcaaatgac gcaatgccgt
     2161 ctgaagcccg attcggcgtt cagacggcat tgaggctgaa atccaaaaaa ctcaaagcca
     2221 caggaattta tccggaaaaa caaaaaacac aggaattcat cgggaaaaac aacaatcttt
     2281 ccaccgtcat tcccacgaaa gtgggaatct tgatccgtcc gcacggaaac ttatatttcg
     2341 tcatttgcac ccgacataat tcaatacttt attttgcaat aaattaacgg atgctgacaa
     2401 attagattag attaattacc ctattatcta tattgggtta tcgggttgct aattgaacag
     2461 agcgtagcaa accataaagt gagtgtttcc acccactctg catttactat gaagtggtta
     2521 ttaaatatga taatgagacc tattaaattt agtatggtaa atacgttatt atttattgtt
     2581 atatgtagtt cattttttga tctgctcgtt caattatgta caattttatt tcatagccaa
     2641 aaaatatact ttattacatt atttttatta tttattttta attttgttac aaaatctatc
     2701 tatatggcaa ttatttatcc tattttatat ttttttacga taaaaaaata ttacccttac
     2761 tctaggaaag taataattct attatcatta gcattatcta tatattttag ttttatggac
     2821 ttttactttt tttccatata ttcagataac cttagctatg aaacggagcc tttacattta
     2881 tacatcccta ttattattaa ttttttctca cttttagttt ctaattttat tttatctttt
     2941 atcaacaagt aaatgtatat acaaaataca atccaaaaga tcctaagcaa accaccaatc
     3001 ccacaccacc cttaccggtt caaatccgca caaaaaccca catcccgtca ttcccacgaa
     3061 agtgggaatc taggac
//
LOCUS       DAWKOD010000191         3027 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000191 DAWKOD010000000
VERSION     DAWKOD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.191"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..2302
                     /locus_tag="WJK73_001923"
     CDS             44..2302
                     /locus_tag="WJK73_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ7558353.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFSLLKGGPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2315..2980
                     /gene="pgmB"
                     /locus_tag="WJK73_001924"
     CDS             2315..2980
                     /gene="pgmB"
                     /locus_tag="WJK73_001924"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ7558354.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
BASE COUNT          764 a          930 c          785 g          548 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttca taaatgtaca caagaatcat
       61 ggaaatcagc ccttggacgc tgcgttcggc aaaactggaa aaagaacaca aacggctgca
      121 agagagcctg accagcttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
      181 ctattccgcc gacagccact taggcaccta catcgccggc gtgtggttcc ccgacaaaac
      241 ccgcgtcggc tggtggaaaa acggctatcc caaatatttc ggcaaagcca tcaacgcgtt
      301 caatttcagc aaagtcaaaa tctttgtcga cgggcaggaa gtggacttgg cgaaaaacga
      361 cgttgccggc ttctccgtcg aactcgatat gcggcacggc gtgttgcgcc gctcgtttac
      421 cgtattcggt gtgcgtttca atgtgtgcaa attcctgtcc gtcgcgcaaa aagagctggc
      481 ggtcatccgc tgggaagccg tatccgttga cggtaaaacc caccaagtcc gcatcgattc
      541 catcatcgat gccgacgtga aaaacgaaga ctccaactac gaagaaaaat tctggcaggt
      601 attggacaaa ggcgtttcag acagtctctc ctacattgcc gcccaaaccg ttgccaaccc
      661 tttcggcgtg gaacaattta tcgtcaacgc cgagcaaact ttcgccggca gcttcaaagc
      721 cctcggcggc agccaaaccg actggcaagt ctccaattct ttcgaggtcg aagtcggcgg
      781 cacgcccaaa acctttgaaa aacgggttat tgttaccacc agccgcgatt atcagggctt
      841 ggaagcagtg aaagccgcag gccgcgcctt gtcggaaaaa gtcgcaggcg ttgcgtttga
      901 aaccttgctg gacgcgcaca aagcaggctg gctgcaccgt tgggaaatcg ccgacgtggt
      961 catcgaaggc agcgacgaag cgcagcaagg catccgtttc aatctgttcc aactgttctc
     1021 cacctactac ggcgaagatg cgcgcctgaa catcggcccc aaaggcttta ccggcgaaaa
     1081 atacggcggc gcgacctatt gggataccga agcctatgcc gtaccgctct acctcgcact
     1141 ggcggaaccc gaagttaccc gcaacctgct gcaataccgc cgcaaccaac tgccgcaggc
     1201 gcagcacaac gcgcgcgaac agggcttggc gggcgcactc tatccgatgg taacgtttac
     1261 gggtatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gcaacggcgc
     1321 gattccttac gccatctaca actacaccaa ctacaccggc gacgagggct atcttgccaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttctgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcacgg cgtaaccggc ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaca ccaacaccct cgccgcctgg gtattggact acacccgcga
     1561 agccctggcg aaatacccgc gcccggattt gaacgtgcgt gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gcatgacgaa gaactcggcg tattcgtcca
     1681 acacgacggc ttcctcgaca aagacatccg tcccgtgtcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaagcaggc
     1801 ggacgtgttg caaggcatct acttcttcgg cgaccgtttc aatatcgacg aaaaacgccg
     1861 caacttcgat ttctacgaac cgatgaccgt gcatgaaagc tcgctgtcgc cctgtatcca
     1921 ctctattctc gccgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggaca actacaacaa cgacacagaa gacggcctgc atatcacctc
     2041 catgaccggt tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cttggggcgg
     2101 caaactcagc ttcgcaccgt tcctgccggg cgcgtggaca ggctatgcct tccacatcaa
     2161 ctaccgaggc cgtctgatta aagtcgccgt cggcaaagaa aacgttgtct tcagcctgct
     2221 caaaggcggg ccgctcgatt tgcaggtgta cggcaaagac atcacgctca acggcagcca
     2281 caccgccgcg ttggaaaaat aaggagggcg caaaatgacg tttaccgcag tcctcttcga
     2341 tctcgacggc gtgattaccg ataccgccga ataccactac cgcgcgtgga aaaagctcgc
     2401 cgaagaactg ggcatcggca tcgaccgcaa gtttaacgaa cagctcaaag gcgtgtcgcg
     2461 cgacgattcg ctcaaacgca tcctcgcgca cggcggcaaa accgtcggcg aagccgagtt
     2521 cgccgaactg acccgccgaa aaaacgacaa ctacgtcgag atgattcagg cagtcaaacc
     2581 cgaagacgtg taccccggca ttttgccgct gctggagaca ttaaaagcaa acggcaaaaa
     2641 aatcgccctc gcgtccgcca gtaaaaacgg cccgttcctg ctcgaacgca tggggctgac
     2701 ccactttttc gacactgtcg ccgaccctgc cgccgtcgcg cattccaaac ccgcccccga
     2761 catcttcctc gccgccgccg agggcgtggg cgcggacatc cgccactgca tcggcattga
     2821 agacgccgcc gccggcgttg ccgccatcaa agccgccggc gcactgccca tcggcgtagg
     2881 caaagccgaa gacttgggca gcgacatcgc gctggtgtcc gagaccgccg ggctgaccta
     2941 cgcctacctg caaaacgtgt gggcgcagtc gggcaggtaa agcgcgtcag ataaagtgtt
     3001 aaggaagtaa aaggccgtct gaacagt
//
LOCUS       DAWKOD010000192         2995 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000192 DAWKOD010000000
VERSION     DAWKOD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.192"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(230..1219)
                     /gene="pdxA"
                     /locus_tag="WJK73_001925"
     CDS             complement(230..1219)
                     /gene="pdxA"
                     /locus_tag="WJK73_001925"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ7558355.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1379..2806
                     /locus_tag="WJK73_001926"
     CDS             1379..2806
                     /locus_tag="WJK73_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7558356.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          610 a          819 c          848 g          718 t
ORIGIN      
        1 aaaccgaaac cgaacaagcc ggattcccgc aacattccgt cattcccgtg aaaacgggaa
       61 tctagaacct caaactttcg gataatcttt gaatattgcc gtcgcccgaa ggtctggatt
      121 cccgcctgcg cgggaatgac gatttggaaa ttacccgaaa cccaaaaaca actgaaaccg
      181 aacagaccgg attcccgcct gcgcggaaat gacgggtctt ttatcatctt taaaggctgc
      241 cgcgcgccat ctcgacggcg gtctccacgg cagttatcag gctgccggaa tccgccctgc
      301 cggttgccgc caaatcaagc gcggtgccgt gatcgacgga ggtgcggata aagggcaggc
      361 cgagcgtgat gttcacgccc tgtccgaagc tgtggtattt caacacgggc agcccttggt
      421 cgtggtacat cgccaatacg gcatccgcac cttcgagcat aaacggttgg aacaaggtat
      481 ccgccggata cgggccggcg aggtttatcc cttcgcggcg caggttttcg agcgcgggga
      541 taatggtgtc ggtttcttcg tgtccgaggt gtccgccctc gccggcgtgg gggttgagtc
      601 cggcgactag gattttggga tttttgatgc cgaatttgtg ttttaagtcg tgatgcaaaa
      661 tgcgtgcgac gctttcaatc agcggttgcg tgatggcggc ggcaacgtct ttcagcggca
      721 ggtgggtcgt tacgagggcg acgcgcaggc ctttgccggc aagcatcatc acaacctgcc
      781 ccgtgccgct tttttccgcc agatattcgg tgtgtccgct gaaaaaacct gtgcttgcgc
      841 gcgcgtcgtt gatgatgcct ttgttcagcg gcgcggtaac gatgccgtcg aaaatgccgt
      901 ctgaaatgcc tgcgagcgcg gtgtccaaaa gttgcagcac ataggcggcg ttggcgggat
      961 tgagtttgcc cgcctcaacc gcttcgacgg cagggatgtg cagcacttcc agctcgccgt
     1021 atgccgcgcc gcctgattcc ggatcgaagt cgcgcaagac gacgcttttg cccaaggctt
     1081 cggcgcgcgc gcgcaacagg tgtttgtcgc ccaataccgc gcagcggcag ggcaggcgtg
     1141 caaacgccaa gtccaaacaa atatcggggc cgatgccggc aggctcgccg gaagtaacgg
     1201 caaaaacagg ctgtttcatc gtgtttgctc caaacaaaat gcgattctaa cgccgcagcc
     1261 gcgcggcgat gtaaattttt ctgattttgt tgacaatctg ctagaatggg cgtttacaaa
     1321 atttaaaccc tgcttgcata ccgccaatat gtgcgggttt caactttaag gaagcgatat
     1381 gaacgagaac tttaccgaat ggctgcacgg ctgggtcggc gccatcaacg atccgatgtg
     1441 gtcatacttg gtttatatgc ttttgggtac ggggcttttc ttcaccgtaa ccacgggctt
     1501 tgtccaattc cgcctgttcg ggcgcagcat caaagaaatg ctcggcggcc gcaaacaggg
     1561 ggacgaccct cacggcatca cgccgtttca ggcatttgta accggccttg ccagccgcgt
     1621 gggtgtgggc aacatcgcgg gcgtggcaat cgccatcaaa gtcggcgggc cgggcgcggt
     1681 gttttggatg tgggtaaccg ccttaatcgg tatgagttcg gcgtttgtcg aatcttcgct
     1741 ggcgcagctc tttaaagtcc gcgactacga caaccaccat ttccggggcg gccctgccta
     1801 ctacatcacg caagggctgg ggcagaaatg gctgggcgtg ttgttcgccc tgagcctgat
     1861 tttctgtttc ggctttgtgt ttgaagcggt tcagaccaat accatcgccg ataccgtcaa
     1921 agcggcatgg ggttgggagc ctcattatgt cggcgtcgcc ctggtgattt taaccgcgcc
     1981 gattatcttc ggcggcatca ggcgcatatc taaagcggcg gaaatcgtcg tccccctgat
     2041 ggcggttttg tacctcttta tcgcgctttt catcattttg accaatattc cgatgattcc
     2101 ggacgtgttc ggtcagattt tttcgggcgc gttcaaattc gacgcggcag caggcggctt
     2161 actcggcggt ctgatttcgc aaacgatgat gatgggcatc aaacgcggcc tgtattccaa
     2221 cgaggcgggt atgggttccg cgccgaacgc cgccgccgcc gccgaagtga aacaccctgt
     2281 ttcgcaaggt atgattcaaa tgctgggcgt gtttgtcgat accatcatcg tttgttcttg
     2341 caccgccttc atcatcttga tttaccaaca gccttacggc gatttgagcg gtgcggcgtt
     2401 gacgcaggcg gcgattgtca gccaagtggg gcaatggggc gcgggcttcc tcgccgtcat
     2461 cctgtttatg tttgcctttt ccaccgttat cggcaactat gcctatgccg agtccaacgt
     2521 ccaattcatc aaaagccatt ggctgattac cgccgttttc cgtatgctgg ttttggcgtg
     2581 ggtctatttc ggcgcggttg ccaatgtgcc tttggtctgg gatatggcgg atatggcgat
     2641 gggcattatg gcgtggatca accttgtcgc catcctgctg ctctcgccct tggcgtttat
     2701 gctgctgcgc gattacaccg ccaagctgaa aatgggcaaa gaccccgagt tcaaactttc
     2761 cgaacatccg ggcctgaaac gccgtatcaa atccgacgtt tggtaaatcc cgcccttacc
     2821 agagccgctt ccccgcgaag cggcttttcc ctttccgcac actgtaaaaa cagggcgaac
     2881 aagcgtacaa tcccaaccct ttacttttga atccatttcg tttttcagac ggcatattga
     2941 atatagtggt ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaag
//
LOCUS       DAWKOD010000193         2995 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000193 DAWKOD010000000
VERSION     DAWKOD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.193"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            82..243
                     /locus_tag="WJK73_001927"
     CDS             82..243
                     /locus_tag="WJK73_001927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558357.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            293..577
                     /locus_tag="WJK73_001928"
     CDS             293..577
                     /locus_tag="WJK73_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558358.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WJK73_001929"
     CDS             complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WJK73_001929"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ7558359.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWG
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1540..2943)
                     /locus_tag="WJK73_001930"
     CDS             complement(1540..2943)
                     /locus_tag="WJK73_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7558360.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLLDTNSPYQDYKQGIEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNLHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLPEAHWQLGLQYRFGQGTKADTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAQKDSMALYNLACIHYNGHGVGLDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT          629 a          623 c          898 g          845 t
ORIGIN      
        1 atacgattaa agtaatgtcg gattcaagaa tccgacctac ggaaaactaa agattttata
       61 cgggctacgg cttgctggct tatgcgttgg cttaatttga aaaacagcac atcggttcat
      121 aatacacaag gtattagaac aaaagaagca gttgaacagg gattgcgtgg taataataga
      181 cctgaaaaag gaataggtaa tagatggaat tatgatggat ttataaagga attagcgaag
      241 taacaggtat tccaaatatt tctgataaat ataaagaaca aggagtaaaa aaatgaaagt
      301 aatcactatt acattgttta ctagctgcat actatctgga tgctatgggc ttgaatatgg
      361 aatgagtggc ggcattcctc atgatgactg ggcaagaggt agccatgtta gtcaagccaa
      421 agcagatgct gcgtggaaag aatgttctga caaatatatg agaaatggtg gttcaggatt
      481 agtagaacaa tgtatgaatg ataaaggatt aatacgttta tctgaaatac gtcgtaggca
      541 aaagagagca gaaacacaaa gagcagtaat taagtaacaa taatgaaata ctactgttcc
      601 cgaacaaagc ggcatttacg caggtgatga cggctatatt ttgaaaaatg ccgtctgtaa
      661 gcaaacgtgc ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat
      721 accaagtccc aaacgccgtg cccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg
      781 cggtagtccg gggtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctactg
      841 aggacttcaa gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg
      901 ccggttttga gtttgggcag cagtttggcg atgaaggggg cttgtatcag acggcgtttg
      961 tggtggcgtt ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg
     1021 tcttgcagca tattttcgac gacttctacg gcatcgtgcc gcatcacgcg gatattttcc
     1081 aaatggtttt cttctatgag tttgagcagg ttgcctacgc cgggaccgtg tacgtcgatg
     1141 gcgaggaagt cggtttcggg caggcggagg gcgatttctg cggttgctgt ccccataccg
     1201 aagcctattt cgaggatttt ggggcggttg cttccgaaat tggcatcaag atcggccggt
     1261 gttgcttggt agtcgatgcc gaagctgccc cataaggtat cgatggcgcg ttgctgcgcg
     1321 gcggtcatgt ggctttggcg gaggacgaaa ctgcggatgc tgcgggggtg ttcggcttgc
     1381 cagttgtttt gtgtttggtt ttcggcggtg tctgtcataa tgctttcgtg cgcttgtgca
     1441 atcaggatgc cgtctgaatg tgaaaggcct gttcggacgg cgggaaagga aaggggcgat
     1501 tatacctgtt tcgtttcggc agggtaggtg taacgctgtt cagacggcat tttgccattg
     1561 ttgcagcagt tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag
     1621 gtagcggcag gctttttctt tgtccagccc gacaccgtgt ccgttgtaat ggatgcaggc
     1681 gaggttataa agtgccatgc tgtctttttg cgcggcggct ttttcaaacc atttgcgtgc
     1741 ttcattgtag tcggcggtca taccctgtcc gaagtaatac atgctgccca gattggtttg
     1801 ggctttgctg tggccgagtt ctgcggcttc tgtgtagagt ttgagtgcgg ctgcatattc
     1861 gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagcc gttctgccct
     1921 ttgccgcctg atggcggaat ctttaatgcc gccatattgg tcgggatggt ttaacgcgct
     1981 gtcggatatg agtttctgat acgcggcaag gctgccggct tcggctgcct gccgataata
     2041 atgccgggct ttttccgtat cgggggctat gcccaaactg tagcggcaga tgtcgcccag
     2101 tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcgtgat gcagggcaag
     2161 tttgtagttt ctttccggat gtttgccttg caggtagatg tcggcaagtg cggcatgggc
     2221 atcggggtca ttttcctgtg ccgcctgttg aaaccagtga acggcttcat caggagccgt
     2281 aggtaggatg agctcggcaa gcggggtgta ggcgggaatg tatccttgtt gtgcggcggc
     2341 gcgcaaatga ttgacggcct gtgccgtgtc ggctttcgtc ccttgtccga aacggtattg
     2401 cagcccgagc tgccaatgtg cttccggcaa gccttgtgcc gccgcttttc ggtagagcag
     2461 gtgcgccgtt tcaaaatccg gcggcgtgcc gtagcggcag tattgggcaa gcaggtattg
     2521 cccgtggagg ttgttgtttt ttgcgcttgt ttttgcccat ttcagggctt cttcaaaatg
     2581 tcgggacgca tacagaaggt acatcaggcg gacttgtgct tcgggcagtc cttcctctgc
     2641 caacggtctg taaagcggta tcagggtttc cgggcgcgcg ccttccaatt cttggatata
     2701 tgccagattg taacgcgcgt agggatgtcc ctgttcggcg gcgatgcgga gcatttctat
     2761 gccctgtttg tagtcttggt aggggctgtt tgtatcaagc aggtgttttg ccaactcgaa
     2821 tgcggcggcg ggatgacctg ccgaggccgc ttctgtcagc agtcggcgga cttcggtaaa
     2881 gttatattgt ttttgagaca ggcgttctaa ggcctgccga tagggtaagt cgggattgtt
     2941 catatcagat cgttttccgt tttgtcgaat catcgttatt gtgttatgaa gttag
//
LOCUS       DAWKOD010000194         2955 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000194 DAWKOD010000000
VERSION     DAWKOD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.194"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..1418)
                     /locus_tag="WJK73_001931"
     CDS             complement(108..1418)
                     /locus_tag="WJK73_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ7558361.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTVDGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEVLKAK"
     gene            1730..2152
                     /locus_tag="WJK73_001932"
     CDS             1730..2152
                     /locus_tag="WJK73_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558362.1"
                     /translation="MEWVSLFSDPQKDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADPQLGYTPELSVKSNNDILFKNNLQSVDEKPHS
                     GNIHLDAKGKIQFNLNVSDGDGVQDVASGKKYRQTIVP"
     gene            2240..2940
                     /locus_tag="WJK73_001933"
                     /pseudo
     CDS             2240..2940
                     /locus_tag="WJK73_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          813 a          714 c          714 g          714 t
ORIGIN      
        1 ttgtctttat tggctttatc gataaacagg ataagacctg aaaaaagccg atacgccttt
       61 ttggtgtacc ggctttgcca tactgttctg cttcagacgg catcgcttca ttttgccttt
      121 aatacttctt cgtccagcga tttcaaccac gccagctttt cgccgatttt gatttccagt
      181 ccgcgcggga cgggttggta gaaatcgggt tcgtccaggc cgtcgggcat atagctttcg
      241 ccggcggcgt aggcgttcgg ttcgtcgtgg gcgtagcggt attcgcgtcc gtagcccaat
      301 tccttcatca gcttggtcgg ggcgttgcgc aggtggacgg gcacttcgtc gctggcgttt
      361 tctttgacga agtggcgcat ttggttgtat gccttgtagc ccgcgttgga tttcgcggcg
      421 gcggcaagat acaataccgc ttgcgccaaa gccagttcgc cttcgggcga gcctaagcgt
      481 tcgaaggtgg cggcggcatc gttggcgatt tgcaaggcgc gcgggtcggc aagcccgatg
      541 tcttcccaag cgatacgcac gatgcggcgg gcgaggtagc gcgggtcggt gccgccgtcg
      601 agcatacggc agaaccaata cagcgcggcg ttcggatgcg aaccgcgcac ggatttgtgc
      661 agggcggaga tttggttgta gaaactctcg ccgcctttgt cgaaacggcg gatttgcgcc
      721 ccgagactgt cggcgagaaa ttcggcggtt aagtttttca gacgacgtgt atcggcggcg
      781 cgtaaaagtt gttccaacaa attcaacaat ctgcgcgcat caccgtcgac ggtattcacg
      841 agtaattttt gcgcatccgt ttcaatcgta aactcttggt attcaggcaa agccaatacc
      901 ttggcaatca gctttttcag gtcgtctgaa gacaagggtt gcaaaacata cacctgagcg
      961 cggctcaaca gcgcgggatt gacttcaaac gacggatttt ccgtcgtcgc accaataaag
     1021 gttagcaaac cgctttcgac atgcggcaaa aacgcgtcct gctgcgcctt gttgaagcgg
     1081 tggacttcat cgacaaacaa aatcgtcgcg cgtccctgct gcaaagcgat ttcggcttta
     1141 tcgattgcct cgcggatgtc cttcacgccg gaaaatacgg cggaaacagg caaaaactgg
     1201 gcgttgaaac tctgcgccaa aatccgcgcc aacgtcgtct tgcccacgcc cggcggcccc
     1261 cacagcaaca tagaatgcgg cttgccgcct tctaccgcca cgcgcaaagg tttaccttcg
     1321 ccgatgaggt gttcctgccc caccacgtcg tcaagcgtat gcggacgcaa tcgttcggca
     1381 agcggcgcgt cgggttctcg ggcaaacaaa tcggtcataa cggctccgtc aacaggtttt
     1441 caaacaatat gattatacgg cagggaacgg cggcgtgccg catacggatt ccgcccctcc
     1501 gtttgcctta agccgatatt aggcgcatac tggaaaagac gagagacttc acacaatata
     1561 tccggcacgg agaccgattc cgcaccggca tgacaatacc caaatcagcg tttcaattaa
     1621 acattaagga gactaaaata gaaaatttgc tttatctacc attgctttgt tgattcaatc
     1681 ggcattatgt tttgaggcgg aagcccatga atatactaat attcaagaga tggaatgggt
     1741 gtctttattt tctgatccgc aaaaagacga tgatagtctt ataaccctta aagatgaaaa
     1801 aatcactgta aaaaactata ttgtgccttg gtggaaaaaa ggtgaaaact ttagaaaatt
     1861 agaacttatt accactggta aaaatgataa tggaaattta acggtaaatg gaatttacgg
     1921 catccaatct tttgcagacc cgcaattagg atatacgcct gaattatcgg tgaaaagcaa
     1981 taatgatatt ctatttaaaa ataatctcca atctgttgac gagaagcccc attccggaaa
     2041 tatccatttg gatgccaaag gaaaaattca atttaatttg aatgtttccg atggtgatgg
     2101 ggtacaagat gtagccagtg gaaaaaaata ccgacaaaca atagtgcctt agtgcggtat
     2161 gtcaacacta tcagcccccc ccgatgaaaa tgacaaaggc agcatatcct ttcattcaaa
     2221 aacaggcaat ttgttcaata tgaacgttaa cgttcataaa gacacacacc atttcggtat
     2281 tcacaacctc aacaataccg atgttcatct gactgccgaa gacagcgata atgtggttgc
     2341 gattaaaggg gaactcgccg accctatgcc gacaggaaat gttgagagta caagtttttt
     2401 aaggggcatt gaaacggctt ttaaatccaa taccgtatta acggggaata acaataaaat
     2461 atccatcgcc actgagggca agggcttggg attgggcgta aatggtctcc agtccaactc
     2521 cccctattcc ggacacacga cgggcatttt catcaataat caatcaagcg accttaccca
     2581 accttccctt gatggcatca gcaccatcaa actcaaagcc gtcaagggaa ataatgaagt
     2641 tgatttaaat gtaaaaaacc acgcctccgt aaaaggcatt atcgcgtcgc attctgcaaa
     2701 agcaacgctt gaagccgaag gggacaatat tgtccaggtt aaaaatccgg acaccaatac
     2761 ggctttaatc gatgctttga aaaaacaata ccccaatgct tcgaaagaac tatacagggt
     2821 gggggttcaa tccacgactg gaacagtacg catttcgcca aacggcaaaa tcgcattaac
     2881 ggaattgaaa acttttggaa ttgtacaaaa cggcatttgc gtcagtttat agtggattaa
     2941 caaaaaccag tacgg
//
LOCUS       DAWKOD010000195         2947 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000195 DAWKOD010000000
VERSION     DAWKOD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.195"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            162..1274
                     /gene="nadA"
                     /locus_tag="WJK73_001934"
     CDS             162..1274
                     /gene="nadA"
                     /locus_tag="WJK73_001934"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ7558363.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1326..2834
                     /gene="nadB"
                     /locus_tag="WJK73_001935"
     CDS             1326..2834
                     /gene="nadB"
                     /locus_tag="WJK73_001935"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ7558364.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASHH
                     AQGGIAAAWSREDDIEKHVADTLEAGAGLCNEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPTLQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYARRQNIGAHFNSDC"
BASE COUNT          691 a          907 c          814 g          535 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcaggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aagttgagca taatataaaa cagtttgaaa aacagacggc
      121 aaaccttcat gccgtctgaa aaccagctac aaggaaacat catgcaaacc gccgcccgcc
      181 gctcgttcga ttacgatatg cccctcatcc aaaccccgac ttccgcctgc caaatccgtc
      241 aggcgtgggc gaaggttgcc gacacgcccg accgcgagac ggcaggccgt ctgaaagacg
      301 aaatcaaggc tttgctgaag gagaaaaacg cggtcttggt ggcgcattat tacgtcgatc
      361 cgctgattca ggatttggct ttagaaacgg gcggatgcgt gggcgattcg ctggaaatgg
      421 cgcgcttcgg cgcggaacac gaagctagta cattggtggt ggcgggtgtg cgcttcatgg
      481 gcgagagcgc gaaaatcctc tgccctgaaa aaacagtgct gatgcctgat ttggaggcgg
      541 aatgttcttt ggatttgggc tgtccggaag aagcgttttc agcgttttgc gaccaacacc
      601 ccgaccgcac ggtcgtagtg tacgccaaca cttccgccgc cgtgaaagcg cgtgccgatt
      661 gggtggtaac gtcttcggtg gcgttggaaa tcgtgtcgta tctgaaatcg cgcggcgaga
      721 agctgatttg gggacccgac cgccacctcg gcgactacat ctgccgcgaa acgggcgcgg
      781 atatgctgtt gtggcagggt tcgtgcatcg tccataacga attcaaaggg caggagctgg
      841 cggcgttgaa agcggaacac cccgacgcgg tggtgctggt tcatcccgaa tcgccgcaaa
      901 gcgtcatcga actgggcgac gtggtcggct cgaccagcaa actgctcaaa gccgccgtat
      961 ctcgtcccga aaaaaaattc atcgtggcga ccgatttagg catcctgcac gaaatgcaaa
     1021 agcaggcgcc cgacaaagaa tttatcgccg cgccgacggc gggcaacggc ggaagctgca
     1081 aaagctgcgc gttctgcccg tggatggcga tgaattcgct gggcggcatc aaacacgccc
     1141 tgacaagcgg acgcaacgaa atcctgttgg acaggaagct gggcgaagcc gccaaactgc
     1201 ctttgcagcg tatgctcgac ttcgcggcag gactcaagcg cggggatgtg ttcaacggca
     1261 tggggcccgc ctgatttgcc gtcaatattg ccgtttcaga cgacctctca aacaaggaca
     1321 acaccatgca aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc
     1381 tcgccctgtc gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg
     1441 acaccgccag ccatcatgcg caaggcggga ttgcggcggc gtggtcgaga gaggacgaca
     1501 tcgaaaaaca cgttgccgat actttggaag cgggcgcagg tttgtgcaac gaagccgccg
     1561 tccgcaccat cctgtcgcag ggcaaaccgg caatcgaatg gctgctggcg cagggcgtgg
     1621 cgttcgaccg aaattatgac ggtctgcacc tgacacgcga aggcgggcac acctgccgcc
     1681 gaatcgccca cgtcgccgac tacacgggcg aagccgtcat gcagagcctg attgcccaaa
     1741 tacgccgccg cccgaacatc cgcgtttgcg agcggcagat ggcgttggac attcaaaccg
     1801 aatcaggcgc ggcgtgcgga ctgaccgtcc tcgactgccg aacgcaagaa acccaccgca
     1861 tccgcgcccg ccataccgta ctcgcaggcg gcggcttggg gcagatttac gccgccacca
     1921 ccacgccgcc cgaatgcacg ggcgacgcca tcgctatggc gatacgcgca ggctgcgcag
     1981 tcgaaaacct cgaatttatc caattccacc ctacaggctt ggcaaggccg tctgaaaacg
     2041 gccgcacctt cctgatttcc gaagccgtgc gcggcgaagg cggcatcctg accaaccaag
     2101 cgggcgaacg gtttatgccg cattacgacc gccgcgccga actcgcaccg cgcgacatcg
     2161 ttgcccgcgc cattgccgcc gaaaccgcca aacaaacgca agacttcgtc tcgctcgaca
     2221 tcagccatca acccgcagcg ttcgtccgcc agcattttcc gtccatccat cgacactgtt
     2281 tatcccaatg cggcttggac atcacgcgcc aagccatccc cgtccgcccc gtgcaacact
     2341 acacctgcgg cggcatccaa accgacccca gcggcagaac ttccctgccg cagctctacg
     2401 ccttaggcga aaccgcctgc acagggttgc acggagccaa ccgcctcgcc agcaactccc
     2461 tactcgaatg cgtcgttacc gccaggcttg ccgcccaaac catcgcggat ggacaagcgt
     2521 tccaaaccgt accgtccaaa aggtcgtctg aaaacacctc cgccgaagca gacatctttt
     2581 cagacggcat ccaaaacaca ttcagccgcc ccaccctgca agcgttcaac caacgccatc
     2641 tgggcatcct ccgcaacgat accggcctgc gccgcgccat cgcccaactg cggctttgga
     2701 agcaaaacca aaccgagccg cacactgcgt ccgaatacga aaaccgcaac ctgctcgaat
     2761 gcagcctcgc cgtcgcccaa gccgcatacg caaggcggca aaacatcggc gcgcatttta
     2821 atagtgattg ttaaggttgg caggtcagag aaaatcgttc aaataaattt gaatagcatg
     2881 gttaagaaat tggcattcgg tttttagggt tgcgtaagtt gggttatcaa tctgttctaa
     2941 aagcaga
//
LOCUS       DAWKOD010000196         2919 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000196 DAWKOD010000000
VERSION     DAWKOD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.196"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            247..570
                     /locus_tag="WJK73_001936"
     CDS             247..570
                     /locus_tag="WJK73_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7558365.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            684..2861
                     /locus_tag="WJK73_001937"
     CDS             684..2861
                     /locus_tag="WJK73_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7558366.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRPADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          713 a         1028 c          737 g          441 t
ORIGIN      
        1 catcgaacgg ctgaatcaaa gattcagacc gatggcagtc cgcacccgag tttatgcggc
       61 aaacagcgag gctacggcaa cccgtcccct ctccctgtgg gagagggtta gggagagggc
      121 ggtaagccgc aggcttacat caaagccgat aacgcttccg ttacaactcc gcccactgaa
      181 agcagcctgc aacgaagcca aaacgacaaa ccgcatcgta aaccacccaa cccataggag
      241 aaccccatgc aaaacgaaac catcaacctg aaacagcacc ttgccgccat caaagaatac
      301 tggcagcccg aaatcatcaa ccgccacggg ttccaattcc gcttggtcaa acttttgggc
      361 gattacggct ggcatacgca cggatacagc gacaaagtgc tgtttgccgt ggagggcgac
      421 atggcggtgg acttcgccga cggcggcagc atgacgatac gcgagggcga gatggcggtc
      481 gtgccgaagt cggtgtcgca ccgcccgcgt tcggaaaacg gctgctcgtt ggtgctgatt
      541 gagttgtccg acccgtccga ggccgtctga aaacgaagtt tccgaaggaa gctgagtttc
      601 tgcgaagcta aaagcagcct gcaccttcaa tcaatatgcc gaaaatacaa ccccaccgca
      661 caccaacaca caaaggaaat cccatgacac gcttcaaata ttccctgctg tttgccgccc
      721 tgttgcccgt gtacgcgcag gccgatgttt ctgtttcaga cgaccccaaa ccgcaggaaa
      781 gcactgaatt gccgaccatc accgttaccg ccgaccgcac cgcgagttcc aacgacggct
      841 acactgtttc cggcacgcac accccgctcg ggctgcccat gaccctgcgc gaaatcccgc
      901 agagcgtcag cgtcatcaca tcgcaacaaa tgcgcgacca aaacatcaaa acgctcgacc
      961 gcgccctgtt gcaggcgacc ggcaccagcc gccagattta cggctccgac cgcgcgggct
     1021 acaactacct gttcgcgcgc ggcagccgca tcgccaacta ccaaatcaac ggcatccccg
     1081 ttgccgacgc gctggccgat acgggcaatg ccaacaccgc cgcctatgag cgcgtagaag
     1141 tcgtgcgcgg cgtggcgggg ctgctggacg gcacgggcga gccttccgcc accgtcaatc
     1201 tggtgcgcaa acgcccgacc cgcaagccat tgtttgaagt ccgcgccgaa gcgggcaacc
     1261 gcaaacattt cgggctggac gcggacgtat caggcagcct gaacaccgaa ggcacgctgc
     1321 gcggccgcct ggtttccacc ttcggacgcg gcgactcgtg gcggcggcgc gaacgcagcc
     1381 gcgatgccga actctacggc attttggaat acgacatcgc accgcaaacc cgcgtccacg
     1441 caggcatgga ctaccagcag gcgaaagaaa ccgccgacgc gccgctcagc tacgccgtgt
     1501 acgacagcca aggttatgcc accgccttcg gcccgaaaga caaccccgcc acaaattggg
     1561 cgaacagccg ccaccgtgcg ctcaacctgt ttgccggcat cgaacaccgc ttcaaccaag
     1621 actggaaact caaagccgaa tacgactaca cccgcagccg cttccgccag ccctacggcg
     1681 tagcaggcgt gctttccatc gaccacaaca ccgccgccac cgacctgatt cccggttatt
     1741 ggcacgccga cccgcgcacc cacagcgcca gcgtgtcatt gatcggcaaa taccgcctgt
     1801 tcggccgcga acacgattta atcgcgggta tcaacggtta caaatacgcc agcaacaaat
     1861 acggcgaacg cagcatcatc cccaacgcca ttcccaacgc ctacgaattt tcccgcacgg
     1921 gtgcctatcc gcagccatca tcgtttgccc aaaccatccc gcaatacggc accaggcggc
     1981 aaatcggcgg ctatctcgcc acccgtttcc gccccgccga caacctttcg ctgatactcg
     2041 gcggcagata cagccgttac cgcaccggca gctacgacag ccgcacacaa ggcatgacct
     2101 atgtgtccgc caaccgtttc accccctaca caggcatcgt gttcgacctg accggcaacc
     2161 tgtcgcttta cggctcgtac agcagcctgt tcgtcccgca atcgcaaaaa gacgaacacg
     2221 gcagctacct gaaacccgta accggcaaca atctggaagc cggcatcaaa ggcgaatggc
     2281 ttgaaggccg tctgaacgca tccgccgccg tgtaccgcgc ccgtaaaaac aacctcgcca
     2341 ccgcagcagg acgcgacctg agcggcaaca cctactaccg cgccgccaac caagccaaaa
     2401 cccacggctg ggaaatcgaa gtcggcggcc gcatcactcc cgaatggcag atacaggcag
     2461 gttacagcca aagcaaaacc cgcgaccaag acggcagccg cctgaacccc gacagcgtac
     2521 ccgaacgcag cttcaaactc ttcactgcct accactttgc ccccgaagcc cccagcggct
     2581 ggaccatcgg cgcaggcgtg cgctggcaga gcgaaaccca caccgaccct gccacgctcc
     2641 gcatccccaa ccccgccgcc aaagcccgcg ccgccgacaa cagccgccaa aaagcctacg
     2701 ccgtcgccga catcatggcg cgttaccgct tcaatccgcg cgccgaactg tcgctgaacg
     2761 tggacaacct gttcaacaaa cactaccgca cccagcctga ccgccacagt tacggcgcac
     2821 tgcggacagt gaacgcggcg tttacctatc ggtttaaata aggtcgtctg aaaacggagt
     2881 ttctgcgaag ctatagtgga ttaacaaaaa ccagtacgg
//
LOCUS       DAWKOD010000197         2884 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000197 DAWKOD010000000
VERSION     DAWKOD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.197"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            651..2822
                     /locus_tag="WJK73_001938"
     CDS             651..2822
                     /locus_tag="WJK73_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7558367.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKEYEAGKGFRNFNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDASSRTNITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVVGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTDEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHELDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSSSHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          777 a          824 c          657 g          626 t
ORIGIN      
        1 cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
       61 gagtgagtcg gttccgcact atccgtactg tctgcggctt cgccgccttg tcctgatttc
      121 cgttcgtttt cggttattcc cgataaatta ccgccgtttc tcgtcatttc tttaaccctt
      181 cgtcattccc gcgcaggcgg gaatctagtt ttttaagttc cagttgtttc tgataaattc
      241 ttgcagcttt gagttcctag attcccactt ttgcgggaag gacgcggaaa ggttgctttt
      301 cgtcattccc gcgcaggcgg gaatccagtc tgttcggttt cggttttttc cgatagattc
      361 ctgccgcgtt aggggttcta gattcccgct ttcgcgggaa ggacgcggaa aggttgcttt
      421 tcgtcattcc cgcatttgtt aatccactat atttccgccg ttttttacat ttccgacaaa
      481 acttgtcaac aaaaaacaac acttcgcaaa taaaaacgat aatcagcttt gcaaaaatcc
      541 cccccccccc tgttaatata aataaaaata attattaatt atttttctta tcctgccaaa
      601 ccttaacggt ttggatttac ttcccttcat acactcaaga ggacgattga atgaataccc
      661 cattgttccg tctcagcctg ctctcgctta ccctggcggc aggttttgcc cacgcggcag
      721 aaaataatgc caaggtcgta ctggataccg ttaccgtaaa aggcgaccgc caaggcagca
      781 aaatccgtac caacatcgtt acgctgcaac aaaaagacga aagcaccgca accgatatgc
      841 gcgaactctt aaaagaagag ccttccatcg atttcggcgg cggcaacggc acgtcccaat
      901 tcctgacgct gcgcggcatg ggtcagaact ctgtcgacat caaggtggac aacgcctatt
      961 ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct ttggttaaag
     1021 tcgtttccgt acaaaaaggc gcgggttctg cctctgccgg tatcggcgca accaacggcg
     1081 cgattatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat aaaaactggg
     1141 gcgtgcgcct caacagcggc tttgccagca acgaaggcgt aagctacggc gccagcgtat
     1201 tcggaaaaga gggcaacttc gacggtttgt tctcttacaa ccgcaacgat gaaaaagaat
     1261 atgaagccgg caaaggcttc cgtaatttca acggcggcaa aaccgtaccg tacagcgcac
     1321 tggacaaacg cagctacctg gccaaaatcg gaacaacctt cggcgacggc gaccaccgca
     1381 tcgtattgag ccacatgaaa gaccagcacc gaggcatccg taccgtccgt gaagaattta
     1441 ccgttggcga cgcaagttca cggacaaata ttacacgcca agccccatcc taccgcgaaa
     1501 ccacacaatc caacaccaac ttggcgtaca ccggcaaaga tttgggcttt gtcgaaaaac
     1561 tggatgccaa cgcctatgtg ttggaaaaga aacgctattc cgccgatgac aaagataacg
     1621 gctacgcagg caatgtggtc ggccctaacc acacccgaat caccactcgg ggtatgaact
     1681 tcaactttga cagccgcctt gccgaacaaa ccctgttgaa atacggtatc aactaccgcc
     1741 atcaggaaat caaaccgcaa gcgtttttga acggcgaatt tgagatctcc ggtaagaaga
     1801 aagatccgaa agatcccaaa aaagaaatag ataagaccga tgaagaaaaa gcgaaagaca
     1861 agaaagatat ggatcttgtc cattcctaca aactgtccaa cccgaccaaa accgataccg
     1921 gcgcgtatat cgaagccatt cacgagcttg acggctttac cctgaccggc gggctgcgtt
     1981 acgaccgctt caaggtgaaa acccacgacg gcaaaaccgt ttcaagcagc aaccttaacc
     2041 cgagtttcgg cgtgatttgg cagccgcacg aacactggag cttcagctca agccacaact
     2101 acgccagccg cagcccgcgc ctgtatgacg cgctgcaaac ccacggcaaa cgcggcatca
     2161 tctcgattgc cgacggcacc aaagccgaac gcgcgcgcaa taccgaaatc ggcttcaact
     2221 acaacgacgg cacgtttgcc gccaacggca gctacttctg gcagaccatc aaagacgcgc
     2281 ttgccaatcc gcaaaaccgc cacgtctctg ccgccgtccg tgaagccgtc aatgccggtt
     2341 acatcaaaaa ccacggttac gaattgggcg cgtcctaccg caccggcggc ctgactgcca
     2401 aagtcggcgt aagccacagc aaaccgcgct tttacgatac ccatcctaaa aaactgttga
     2461 gcgcgaatcc tgaatttggc gcacaagtcg gccgcacttg gacggcttcc cttgcctacc
     2521 gcttccaaaa cccgaatctg gaaatcggct ggcgcggccg ttatgttcaa aaagccgtgg
     2581 gttcgatatt ggtggcaggt caaaaagacc gcaacggcaa attggaaaac gttgtacgca
     2641 aaggtttcgg tgtgaacgat gtcttcgcca actggaaacc gctgggcaaa gacacgctca
     2701 atgttaatct ttcggttaac aacgtgttca acacgttcta ctatccgcac agccaacgct
     2761 ggaccaatac cctgccgggc gtgggccgcg atgtacgctt gggcgtgaac tacaagttct
     2821 aaaacgcaca tcccgaaaaa atgccgtctg aaagcctttc agacggcatc tgttctgata
     2881 attt
//
LOCUS       DAWKOD010000198         2615 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000198 DAWKOD010000000
VERSION     DAWKOD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.198"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            130..249
                     /locus_tag="WJK73_001939"
     CDS             130..249
                     /locus_tag="WJK73_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7558368.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            262..1125
                     /locus_tag="WJK73_001940"
     CDS             262..1125
                     /locus_tag="WJK73_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ7558369.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(1176..2228)
                     /gene="sohB"
                     /locus_tag="WJK73_001941"
     CDS             complement(1176..2228)
                     /gene="sohB"
                     /locus_tag="WJK73_001941"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ7558370.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2332..2421)
                     /locus_tag="WJK73_001942"
     tRNA            complement(2332..2421)
                     /locus_tag="WJK73_001942"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2385..2387),aa:Leu,seq:taa)
     gene            complement(2524..2597)
                     /locus_tag="WJK73_001943"
     tRNA            complement(2524..2597)
                     /locus_tag="WJK73_001943"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2563..2565),aa:Cys,seq:gca)
BASE COUNT          618 a          681 c          636 g          680 t
ORIGIN      
        1 gcccggatgc ctgattatcg ggtatccgag gaggattagg ggggtattgg ggtaaaatta
       61 gtggatattt gaaacgaaaa cagccgaaaa cctgtgtttg ggtgtcagct gtcgggaggg
      121 aaaggaattt tgcaaaggtc tcacaaagta tttgccacat tcggcgacaa gcgcacaacc
      181 cggctgtttg ccgatttgga tgcactggct gaagtgatgg aaaaaacaat ctcggaaaat
      241 aaaaaatagg ggggcaaata tgtggaaaat gttgaaacac atagccaaaa ccaccgcaag
      301 cgattgattg gcacattttt tctggtcgga ctggaaaacc ttttgatgct ggtgtatccg
      361 gtgtttggcg gctgggcgat taatgccgtg attgcggggc aggcgtggca ggcgttgctg
      421 tacgctttgg ttgtgctttt gatgtggctg gtcggtgcgg cgcggcggat tgccgatacg
      481 cgcacgttta cgcggattta taccgaaatc gccgtgccgg ttgtgttgga acagcggcag
      541 cggcaagtcc cgcattcggc ggtaactgcg cgggttgccc tgtcgcgtga gtttgtcagc
      601 ttttttgaag aacacctgcc gattgccgcg acatccgtcg tatccatatt cggcgcgtgc
      661 atcatgctgc tggtgctgga attttgggtc ggcgtgtcgg cggtgggcat acttgcgttg
      721 tttttatggc ttttgccacg ttttgccgcc atcagcgaaa acctgtattt ccgcctgaag
      781 aacagcttgg aacgcgacaa ccactttatc cgaaaaggcg acgagcggca gctggaccgc
      841 cattacggac tgcttgcgcg cctgcgtgtg ctgatttcca accgcgaagc cttcggctat
      901 ctctgcgtcg gcacggcgat gggtattttg ttcggctttg cttttgtgat gatgacgctc
      961 aaaggctaca gcagcgcggg gcatgtctat tcggtcggca cttatctgtg gatgtttgcc
     1021 atgagtttgg acgacgtgcc gcgattggtc gaacaatatt ccaatttgaa agacatcgga
     1081 caacggatag agtggtcgaa acggaacatc aaagccggaa cttgaaaaat gccgtctgaa
     1141 cacgcttcag acggcatttc catccgttcg gcaaactaca tcacatccgc ccgccggttg
     1201 acaagtttgg caaacaactt ttcaacagaa gcttccgcct gcaaaccaat gcgctggatc
     1261 aggctttgct tctcctgata tttcacttcg ataacctgtt tgttttcaaa cgctttcaac
     1321 aacaaatcat cactggtcga aatctcgtca atcaagttca acgccaacgc ctgccgaccg
     1381 aaccaatgct cgcccgttgc cacttcctca atatccaatt gagggcggtt ctcgctgaca
     1441 aactgcttga acaactgatg cgtttcctcc agttcctgtc ggaatttctg tttgcccttt
     1501 tccgtatttt cacccataaa agtaaccgtg cgcttaaatt cgcccgccgt catcacatcc
     1561 acatcaatat catgtttttt caacaggcgg tggatattcg gtacttccgc tacaacaccc
     1621 accgaaccga caatcgcaaa cggagcggaa acaattttat ccgccacaca cgccatcata
     1681 taaccaccgc tcgccgccac cttatcgacg gcgacggtca gcggaatatt gcgttcgcgc
     1741 aaacgcctaa gctgcgaagc cgccaaaccg taaccgtgaa ccacgccgcc cggactttcc
     1801 aatctaagca gaacctcatc ttcaggcttg gcaatcaaaa gcaccgccgt aatctcatga
     1861 cgcaaggatt ctacggcgtg tgcatacaaa tcgccgtcaa aatccaacac aaaaaggcgg
     1921 gatttttgcg tttcggaaga tttctcccca ccctccttca aacgcttttt ctctgctttg
     1981 gcttccgcct tttccttttt cttttcctct ttttcctgat gttttgcctc ttccccgctt
     2041 aaaaagaatg cttcaaacga ttgccgctgt tttttataat tttccgaaaa atccgtcagt
     2101 acgacactgc cgctttccga ctgtttctta ctctgtacga tagccaacac aatcagcgca
     2161 attgcgccga acacggtaag cagttcgagc aggaaaatac cgtaattcag taaaatttct
     2221 ttccacattg attggatttc ctcttgttca ggcatgaaca tgtcaatatt gtccatcacc
     2281 gtccgacaga taaaaaaata accgcttgga gcggcattgt cattttcagc ttggtgcccg
     2341 gagccggaat cgaaccggca cgggatgttt agtcccgacg gattttaagt ccgttgtgtc
     2401 tacctatttc accacccggg catttgtgaa aggtggaggc gggggcgcgg attttaaccg
     2461 gcctatatga agattgcact cctcatagca taaacactct gccaccccgc cgtagtacga
     2521 taatggaggc gagagtcgga atcgaaccgg cgtagacgga tttgcaatcc gctgcataac
     2581 cactttgcta tctcgcccta aaactggctt atcta
//
LOCUS       DAWKOD010000199         2596 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000199 DAWKOD010000000
VERSION     DAWKOD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.199"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            373..885
                     /locus_tag="WJK73_001944"
     CDS             373..885
                     /locus_tag="WJK73_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ7558371.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="WJK73_001945"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="WJK73_001945"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ7558372.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            2381..>2596
                     /locus_tag="WJK73_001946"
     CDS             2381..>2596
                     /locus_tag="WJK73_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558373.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMDKECNRE"
BASE COUNT          667 a          766 c          605 g          558 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gccaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cgttctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgctggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcggttt cggtcgtttc cgataagttc
     2161 ccgttgcttt tcatttctag attcccactt tcgcaggaat gacggtggaa aggtttttgt
     2221 tgtttcggag aagtttctgc aaccctagaa tctcgttatt tccacaaaaa acagaaaccc
     2281 aaaacagcaa cttaaaacct tatatattgc gctccataag gggcgcatat ttttaatcct
     2341 tgtaaagccc cttaactcta gccagtgaag gggcttaatc atgtttattt tctatactgt
     2401 caatcccgaa cacgtatatt tccccaaagc atacatcatg aaagtgttca aggataaagg
     2461 ctacgaatcc caatgcatca caacagtttc tttttatatc tgcaatccga ctttgaaaca
     2521 aaagaccgaa aacgaagcgt atgaatatgg cagattgttt gttaaagaac tgatggataa
     2581 ggaatgcaac cgtgaa
//
LOCUS       DAWKOD010000200         2592 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000200 DAWKOD010000000
VERSION     DAWKOD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.200"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(207..278)
                     /locus_tag="WJK73_001947"
     tRNA            complement(207..278)
                     /locus_tag="WJK73_001947"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Arg,seq:cct)
     gene            complement(298..744)
                     /locus_tag="WJK73_001948"
     CDS             complement(298..744)
                     /locus_tag="WJK73_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ7558374.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(749..1723)
                     /gene="ispB"
                     /locus_tag="WJK73_001949"
     CDS             complement(749..1723)
                     /gene="ispB"
                     /locus_tag="WJK73_001949"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ7558375.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1739..1927)
                     /locus_tag="WJK73_001950"
     CDS             complement(1739..1927)
                     /locus_tag="WJK73_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558376.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1947..2255
                     /gene="rplU"
                     /locus_tag="WJK73_001951"
     CDS             1947..2255
                     /gene="rplU"
                     /locus_tag="WJK73_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ7558377.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2280..2552
                     /gene="rpmA"
                     /locus_tag="WJK73_001952"
     CDS             2280..2552
                     /gene="rpmA"
                     /locus_tag="WJK73_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ7558378.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          760 a          699 c          575 g          558 t
ORIGIN      
        1 tccactatat cataaaacaa cagatttcca agccggaaca tcttttacgg agcctgaaaa
       61 tcatttcatg attctaccgt cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca
      121 gatggcaaaa aagccctcca acactgaaag gccttatatc ggaaacttcc cgcaacacgg
      181 gaaacagaca aatgcaaatc gtcaaacctc gccaacagga atcgaacctg tattttacgc
      241 ttaggaggca tacgttctat ccgttgaact atggcgagcc gaaatgaaaa ggagatttta
      301 accctttccg acgacaaaag acaagatgcc ggccgcaatc agcggcccga cagggatacc
      361 gcccataaat gccaccccga taaccgtccc gattaacagc cctgtaatta aaacaggctg
      421 ctgtcccatc aaaggcacgc cgcgtcccgc cagccaagcc acgaaaatac cgataaaaac
      481 ggcggatatc attttaaaat tcaaaaattc ggcaacggga ggaacctgcg cctttcctga
      541 aaccaacgga ctcaaaaccc ctatggtcaa aagaatgata ccgagattca acccatgctt
      601 ctcgaccaac gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc
      661 cgagatggta atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccagaaa
      721 caggggaaca aaactgaaat tcatcgcctc aagacaccct gaccaaagat tccttcgcca
      781 gctgaatcat ggcatccttc acttcgctgt cgggcagggc atccaaggcg gtaacggcac
      841 aatcgactgc tttgcgcgcc tcgcctatcg aatatgccaa agcatccgaa cggacgacat
      901 aatcgtggat tttctcaaaa tagctgcgat ctgcattttc caaagcagta cgcacatcgt
      961 tcgcaacctg ttcggaaccc tgacgcatca gataaatcaa aggcaaagtc ggttttcctt
     1021 ccgccaaatc gtcgccgacg tttttgccgg tttcttcggt ttcgccagaa tagtccagca
     1081 catcgtcaat aatttggaat gccgtaccga catacatacc gtagtctttc aacgcccgtt
     1141 cgtgttcggg ggaagccttg cccaaaattg cgccgacttg agcggcagct tcaaacaatt
     1201 ttgccgtttt atattggatg acttggatat attgttcttc ggtaatgtcc gtattgccga
     1261 tgttcatcag ctgcatgact tcgccctcgg caataatgtt ggttgcatcc gccatcactt
     1321 ccaaaacgcg catactgccc gaggcaacca tcagttgaaa ggcgcgcgtg tataaaaagt
     1381 cgccaaccaa cacagccgcc gcattgccga acagattgtt tgccgttgcc cgcccacggc
     1441 gcaaatcgct ttcatcgacg acatcgtcgt gcaggagggt ggaagtgtgg ataaactcga
     1501 ccatcgccgc cagcgaatac agtttctcgt catcataacc gaccgcctta cccgccaaaa
     1561 tcgtcataat cggacgcagg cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg
     1621 aaatcagtgc gacatcggat tgcaccgcac ggttgatgac ttcattgact ttggcaaggt
     1681 cttcaggcag atgtcgctgg aaatagggca gattctcgag catggcagac tctcaggttt
     1741 acgtttccgt caaacggcag ataccgcttg aaagctgatt caaatagcgg gcaattatat
     1801 cagcaaagcc atcagggtac atcttttcag acggcatatg gttatccgaa ccgcgcaaac
     1861 tttgacaaaa caggcaaata acaatacaat gccgagttcc gcacaatggt gtgcggatat
     1921 taaccataac ccttatggag ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt
     1981 ataaagtttc cgtcggcgaa aaattgaaag tagaacagat accagccgaa ctcgacagcc
     2041 aaatcgaact gaccgaagtt ttgatgattg ctgacggcga atctgtaaaa gttggcgcac
     2101 cctttatcga aggtgcaaaa gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag
     2161 tccgcatctt caaaatgcgc cgccgcaaac actaccaaaa acgccaaggc caccgccaaa
     2221 atttcaccca aatcgaaatc gtggcaatcg cctaacctta aaacttagga gcattacaaa
     2281 tggcaagtaa aaaagcaggc ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct
     2341 tgggcgttaa agcctacggc aacgagctga ttcccgcagg ttccatcatc gtacgccaac
     2401 gcggtaccaa attccacgca ggcgacaacg taggtatggg caaagaccac actttgttcg
     2461 ccaaagttga cggttatgtc gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca
     2521 gcatccgtcc ttacaccggt tctgaagaat aatccaaccg atacctgaag ccgcatcttt
     2581 tcacgatgcg gc
//
LOCUS       DAWKOD010000201         2576 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000201 DAWKOD010000000
VERSION     DAWKOD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.201"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..926)
                     /locus_tag="WJK73_001953"
     CDS             complement(120..926)
                     /locus_tag="WJK73_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ7558379.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1147..2517
                     /gene="ffh"
                     /locus_tag="WJK73_001954"
     CDS             1147..2517
                     /gene="ffh"
                     /locus_tag="WJK73_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ7558380.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          697 a          663 c          666 g          550 t
ORIGIN      
        1 attttcgcca aacgctgcac cacctccaac aatcccttcc tccccctcct ctgactggtg
       61 cgcctttgtg aatatgctgt ctgaaactcg gggcttcgga cggcatctgt tggctcttct
      121 tatctgttca gaatgatttc caatacgaac ttgctgccca tataggcaat catcaggctg
      181 acaaatccga tgatggtcca cacggcggct tttttgccgc gccatgcggt catgctgtgc
      241 ttgagcagca gtccgccgta aatcagccat gacaatatgc cgaatacggt tttatgggta
      301 aaggtcatgg gtttgccgaa tacggcttcg gcaaaaaatg ttccgctgac gacggaatag
      361 gtcagcagga tgaaacctgc ccacatggcc tggaacatga gtttttccaa actgagcagc
      421 gacggcagga atcctgcgag cttggagaag ttcctgcggt gcaggctccg attcagcagc
      481 agggtcaaaa cggacaataa tgttgcgatg ccgaacagcc cgtatgcaag cagcgaagtt
      541 ccgatatgca gcataaaggg aaggtcggta atttcatatc ccgagaattt tccaggaaaa
      601 accaaacctg acagcagcat cagtgcggcg caaggataca gcagcaactg cactccgcgc
      661 agcggataaa agaagctgcc ggcaaaataa ataaacagca tcatccaaac aatcaggctg
      721 ccggaatacc cgaagcccat aatgatgatt ttgtcttgaa tgaccggcat aagcagtgcc
      781 gcgccgtgga cggtcaatgc cgcgcccaaa accggcaatt ccgtcttcca cgggtaatcc
      841 cggccgcgcc cctgctgttg gcagtgccat gcaaatgcac ccaatcctgc gtaaaccgcc
      901 gtcaaaaaga tgaaaactgt cggcatggtg gactttctct aatctatact gttgcgctgt
      961 atgcggccgc ttatgaaata ttggaacttt taacgttgga attgtaaaat ccccgtttcg
     1021 ggcaagcctt gacggatttg ccgatatgct gtccggcaca caagccgcat caaattattt
     1081 tgattttatt ttaacaaaga atgcccctga tggggcaagc tattcttatt cagaccaagg
     1141 accagtatgt tagacaattt aaccggccgc ttcagcaatg ttctcaaaaa catccgcggg
     1201 caggccaaac tgaccgaaga caatattaaa gaagccttgc gcgaagtccg cctcgccctg
     1261 cttgaggcgg atgtcgccct gcctgtcgtc aaagagttcg tcaacaacgt caaagaaaag
     1321 gccctcggtc aggaagtggt gggcagcctg acgccggatc aggcatttat cggcgtggtc
     1381 aacaaagccc tgaccgagct gatgggcagg gaaaacaaaa cgctggattt gtcggttgcg
     1441 ccgcccgccg tcgtgttgat ggcaggtttg cagggcgcgg gcaagacgac gaccgccggc
     1501 aaactcgccc gcctgctgaa aaacgatcag aagaaaaagg ttttggtcgt atctgccgac
     1561 gtttaccgcc ctgccgcgat tgaacaactg cgtctgttgg ccgaacaggt cggcgtggac
     1621 tttttcccat ccgataccaa ccaaaaaccg gttgagattg caactgccgc cgtcgattac
     1681 gccaaaaaac atttttacga tgtattgatg gtcgataccg ccggccgttt ggcaatcgat
     1741 gaggagatga tgaacgaaat caaagccctt cacgcggcgg ttaacccggt ggaaactttg
     1801 ttcgtcatcg atgcgatgct gggtcaggat gcggtgaaca ctgctcaggc atttaatgaa
     1861 gccctgccgc tgaccggtgt cgtattgacc aagatggacg gcgattcgcg cggcggcgca
     1921 gctttgtccg tgcgccacgt aaccggcaag ccgattaaat ttatcggtgt cggcgaaaaa
     1981 atcaacggcc tcgaaccttt ccaccccgac cgtcttgcca gccgtatcct cggcatgggc
     2041 gacgtattga ccctgattga agacgtccaa aaaggtattg atgaagaagc cgccgccaaa
     2101 atggcgaaaa agctacaaaa aggcaaaggc ttcgacctca acgactttaa agaacaaatc
     2161 cagcaaatgc gcaatatggg cggtttggaa aacctgatgt cgaaaatgcc gggcgaactg
     2221 ggtcaaatat cgaaacaaat ccccgaagga acggctgaaa aagcgatggg caaagtggaa
     2281 gccatcatca actcgatgac tccaaaagaa cgcgccaacc ctgccctgct caaagccagc
     2341 cgcaaacgcc gtattgcaat gggtgcgggc acgaccgtac aggaagtgaa caaattgctc
     2401 aaacagtttg aacaaatgca acaaatgatg aagatgttca gcggcaacgg cttgggcaaa
     2461 ctgatgcgta tggcgaaagg aatgaaggga atgaaaggaa tgttcccggg tttgtaagcc
     2521 gatttgaccg aaaacgccgt ctaaaatttc agacggcgtt tttgttttat attctg
//
LOCUS       DAWKOD010000202         2568 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000202 DAWKOD010000000
VERSION     DAWKOD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.202"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(553..2433)
                     /locus_tag="WJK73_001955"
     CDS             complement(553..2433)
                     /locus_tag="WJK73_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ7558381.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          534 a          603 c          752 g          679 t
ORIGIN      
        1 cagaagttgc ccgaaaccaa aaaactgaag ccgaacggtc tggattcccg ctttcgcggg
       61 aatgacggcg cataagttcc cgtgcggaca gacctagatt cccacctgcg tgggaatgac
      121 gattcagaag ttgcccgaaa ctcaaaaaaa acgaaaccga acgaaccgga ttcccgcttt
      181 tacgggaatg acgggatttt gggttgtggt aatttatcgg gaaaacggaa acccctatgc
      241 cgtcattccc gcgcaggcgg gaatctagga cgtagaatct aaagaaaccg ttttatccga
      301 taagtttctg taccgaagaa tctggatttc cgctttcgcg ggaatgacgg catatcagtt
      361 tctgtgcgga taaacctaga ttcccatctg cgtgggaatg acgggatttt agattgcggg
      421 tatttatcgg gaacggcggc ttggaagttc attgaaacgg aaaaacaacg gaaacccaaa
      481 aaaccggatt cccgactgtg ggaatgatga gattcaggtt tctgtttttg ccggagtttg
      541 ccgtatcggg cttcagacgg cattgcctgc cgttgtaccc gcgggtgcga ctgccttgat
      601 gtagttgagc gagacaaact gcttctcggc atctaattcg gtgattttga acaatgcctg
      661 cgatttgggc agcgcgtcaa acggaatacc ggtcgcgcgt gttactagcg gcagaccttc
      721 gatgcggacg aggtcttctt tcagaatcgt cgccgtcagc tcgctcgtgc cttgctgttg
      781 caggtaaacc aggctccagt aggcttccat ttggcgttgg aagtcggcgt aggcggtata
      841 ggcggcatca aagtcgcgca gtgcggcgaa aagctcggca tcgctgtttt gatacagcgg
      901 ctcggcagtg tcgtctatca ggctgatcag ctgcttttgg ttgatgtagt cggcggcgcg
      961 gcgcagcggc gaggtaaacc agccgtaatg ccgcacgccc atgccgatat gcggctcgga
     1021 tttggtgctc atgcgtactt ttccggtggg ttggacgcgg aagaggccgg gcaggtcgtt
     1081 gtcatggagc atttgcgccc aagtgctgtt ggcaagaatc atcatctcgc tgaccagcgt
     1141 atcgatgggc gaaccgcgtt cgcggcggac gacggatacg ttgtcttctt tatccaattc
     1201 gatgctgtaa tcgtattgcg gcgcgcggtc gggttcgtat ttgccgcgcg ctttttgcag
     1261 ggcggtggcg aattgataaa accaaatcag gtcttgatgg tgggcgaaca tcatttcgcc
     1321 cgcttcgtcc aagcccgctt cggcgttgaa atgcggttcg atggcttgga tacgcaggtt
     1381 ttcagcgatg ttgaccgctt cgattttgca ggtcggcgcg ccgacgttga actcgttgtc
     1441 cacatcgaaa taaatgctga tggaagggcg gtatgtacct gcatcaaggc tgaacgcggc
     1501 aatccagttt tcgggcaaca tcgtgatttt gccgccggga aaataaaccg tgcttaagcg
     1561 ttccatgatg tttttttcca ttttgtcgcc ttgcttaatg gcaagcgacg gcgcggcaat
     1621 gtggatgccg acgcgtttta tgccattgcc caaatcggtc aggcttaaag cgtcgtccac
     1681 ttcggtagtc gattcgtcgt caatggaaaa ggcggtaacg tcggctttgg gtaagtcagg
     1741 catttcagga agcggaaggt cggggaagcc tgttcctttg gggaaatatt tgatttcaaa
     1801 cccgtcttgc aggtattggg gaatggacgt aatgccgccc gtttttttcg ccaattcgta
     1861 ggcagaggtt ttcagcgcgt cggcggcttt ggtaaaggct ttgtaggtca gcgactgctt
     1921 gtcgggcgcg tgcaggatgg ttttcaaatc cgccgcgatt tcagacggca tctcgccgcg
     1981 tttcaaggct tccgcccaag cgtcgatttg cgcgtcttgc tgttttttgc gttcgatggc
     2041 ggcaagtgct tgttttaaag tttcttcggg cgcggctttg aacacgcctt tggctttttt
     2101 gtagaaatac atcggcgcgg cgtaaagcgc aatcaaagtt gccgccagct cggttttggt
     2161 cggcgcatgg ccgtaatatt cttcggcgat ggcttcggcg gtaaattcct cttcgccgca
     2221 tacttcccac aataaatcgg tgtcgatgtc cgccgcctgt gcctgcgcgt tttccaaaaa
     2281 cgccgccata tcgccgtcaa actcggcaaa gacgttgttc gccttcactt tggtgcgttt
     2341 gccgtgtggg gtatcgactt ggtaggtggc atcgtttttt tggatgatgg cggcgatttt
     2401 gaattggccg gactcttcgt aaaaaatatt catttttcgg atttttctgt ggaaactcaa
     2461 gcgggcgatt ttagcagatt accgaaaatg ccgtctgaaa aaaggttggg agagggttgg
     2521 cgcgtctttg cggtgcttgc gttatagtgg attaacaaaa accagtac
//
LOCUS       DAWKOD010000203         2496 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000203 DAWKOD010000000
VERSION     DAWKOD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.203"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WJK73_001956"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WJK73_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ7558382.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WJK73_001957"
     CDS             1064..2203
                     /locus_tag="WJK73_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7558383.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAGQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARDLM
                     EAKYTNDPSIFPTKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2335..>2496
                     /locus_tag="WJK73_001958"
     CDS             2335..>2496
                     /locus_tag="WJK73_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7558384.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          676 a          722 c          616 g          482 t
ORIGIN      
        1 gcggcacaca attaaagggc agcgtggcgc gccgcctttt ccggtgggca aaaaatcagc
       61 cctcggaaaa cgcggtttgc aaaatgcaaa ccgcccgtaa cgccgcccat atgattgttt
      121 tgccgcgccg atactttacg ccacacttat tccaacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt ctccgtcgct tccgcacagg
      241 cgggcgcggt ggacgcgctc aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgcccggg cctcttcaaa tgggaataca cttcgcccta caaacagact attgtcggcg
      421 acggtcaaac cgtttggctc tacgatgttg atttggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa tgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg cctcgatgcc gtctgaatgc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggcccgtcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcggcg atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaaaccccc tccgccaagc ccgaagccgg acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa aaagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctacgac ctgaccgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggtgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatatgcc gtgccttatt tctggggaat
     1501 caatactttg gcaatcaata cccagcaggt gaaaaaggca ttgggtacgg acaagctacc
     1561 cgaaaacgag tgggatttgg tgttcaaacc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact atttgggcaa
     1681 agaccccaac agtgaaaatc ctgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccggggcgac gtgaaacgct tcagctcttc cggctatatc gacgatatgg cggcgggcaa
     1801 cctgtgtgcc gccattggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgttgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgtcacct acgcgcccgc
     2041 cagccgcccc gcgcgtgatt tgatggaagc caaatacaca aacgacccat cgattttccc
     2101 gaccaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgccaa
     2161 actgggcgtg aagctgtggc aagggctcaa ggccggaaaa taaccgcaac ctctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg cgtttccccg
     2281 cccgcctgtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcccat ttataatgtc gagagttatt tggaagcgtg tctaaattcc
     2401 atagaaccta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttataaatat atagat
//
LOCUS       DAWKOD010000204         2458 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000204 DAWKOD010000000
VERSION     DAWKOD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.204"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            35..1390
                     /locus_tag="WJK73_001959"
     CDS             35..1390
                     /locus_tag="WJK73_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ7558385.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WJK73_001960"
     CDS             1394..2404
                     /locus_tag="WJK73_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ7558386.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          660 c          740 g          605 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWKOD010000205         2384 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000205 DAWKOD010000000
VERSION     DAWKOD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.205"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1570)
                     /locus_tag="WJK73_001961"
     CDS             complement(119..1570)
                     /locus_tag="WJK73_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ7558387.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1593..2213)
                     /locus_tag="WJK73_001962"
                     /pseudo
     CDS             complement(1593..2213)
                     /locus_tag="WJK73_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
BASE COUNT          744 a          570 c          558 g          512 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaatg acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacaatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcaatccaa cgacgaaaat gcggcgtatg ccaaacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     1081 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     1141 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     1201 cggatttgtt gccatccgta cccacatggt gtacgagcgg gaaaatcaac cctctgacga
     1261 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     1321 accacgcagc acccaaaatc aaaaagtcgg caatacccgc ccacaagaag cactgcccga
     1381 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgatgg
     1441 cgataccggt cagaaaacag gcaagagtaa atgaaaatgc ctgaggcacg gactgtgctg
     1501 cgaacgaaaa catccttacc cacaagactt ctatacccaa gctcaataag ccgctaagga
     1561 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt cactatatcc atcctttctg
     1621 taacgcagcc atactgaagc agcaatcaga aggttaaagc aggctgtcag cgcaatggtt
     1681 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     1741 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     1801 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     1861 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     1921 ccggaaatca gacccctgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     1981 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccaccgaa atacgcacct
     2041 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaacttaa atcgatacct
     2101 atgtgactga atagaagcct ctgccagctg acctagtaaa tcagggcgca gaatccagag
     2161 gcgaaaaaca ccagcgacaa tcctttcagg gttttacttg cggtcgaatt catattttct
     2221 attttcaaat aggcggaact ataaatgaaa tgtatcaatg tgtaaacatt tgtattaacc
     2281 gtaggaaatc accgtattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     2341 acggcgtatc actgttcaaa ctgctgtgcg gcttcacggt gtta
//
LOCUS       DAWKOD010000206         2339 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000206 DAWKOD010000000
VERSION     DAWKOD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.206"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..2257
                     /locus_tag="WJK73_001963"
     CDS             44..2257
                     /locus_tag="WJK73_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7558388.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPIPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          602 a          772 c          559 g          406 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgactg gaacctattg gtttccgaac gctgcaacag
      361 taccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacac cggaagaacg cgcccagcag gcagaaacta tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga acccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgcaccg aacgcctcga atacatcgaa aacttcctca atatcctgcg
      781 tacggaactc aaaaaataca atatccactt tgaagtcgcc ggccgtccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacggcctct ttgacatccg
      901 cgccgtgcgg attctgattg ataccgtccc cgagtgttac accacgctgg gcatcgtcca
      961 cagcctctgg cagcccattc ccggcgagtt cgacgactac atcgccaacc ccaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgccc actggcgtta
     1141 caaagagggc ggcaaaggcg attccgccta cgaacagaaa atcgcctggt tgcgccaact
     1201 cttggactgg cgcgaaaaca tggcggaaag tggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt cctgaccccg cacggcaaag tcctctccct
     1321 gcccacgggc gcgaccccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgcgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctctacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgccca tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacaagcacc
     1981 tgaaaaagtg ctggacgcaa gctgggcggc gttgcaggaa ggacaagtgt tcgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcactcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagcat
     2161 gaggttcacg ctcgaagtca aacaagttac cgacctcccg cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgtataacgc ttcagacggc attttgatta ccagggtttg ctattttttg ttgcatagt
//
LOCUS       DAWKOD010000207         2336 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000207 DAWKOD010000000
VERSION     DAWKOD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.207"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..210
                     /locus_tag="WJK73_001964"
                     /pseudo
     CDS             1..210
                     /locus_tag="WJK73_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            388..1008
                     /locus_tag="WJK73_001965"
     CDS             388..1008
                     /locus_tag="WJK73_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558389.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSVAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVANSLPD
                     GSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            1011..1946
                     /locus_tag="WJK73_001966"
     CDS             1011..1946
                     /locus_tag="WJK73_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ7558390.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          692 a          495 c          555 g          594 t
ORIGIN      
        1 gcgataggcg ctgcagtcgg tgagattgtt ggtgaggctt tggttaagaa tactgatttc
       61 agtcgtatga gtgcgaccga aatcgaaaaa gctaaagcga agattactgc ctattcaaaa
      121 ctggttgccg gcactgcgtc tgccgttgta ggcggggatg tgaatacagc ggcgaatgcg
      181 gcacagatag cggtggagaa taatactttg tatcctagat gcgttggtgc aaagtgtgat
      241 gaatttcaaa aggaacaaca aaaatggata cgtgaaaatc ctgaagaata tcgagaagtt
      301 ttgcttcttc agacaggatt tattccaatt atcggtgata tacagagttt tgtacaagca
      361 cagaccgctg ccgatcacct gtttgctttg ctgggtgtgg ttccgggtat cggtgaatcg
      421 atacaggcct ataaagtagc gaaagcggca aaaaatttac aaggcatgaa aaaagccttg
      481 gacaaggcag caaccgttgc cactgcacag ggctatgtca gcaaaaccaa aatcaaaatc
      541 ggtcaaattg aattaagggt tactgcagca actgacaaac aattgctgaa agctattggc
      601 gaaggaaggg acacgacagg taaaatgacc gagcagttat ttgactctgt agctaaacaa
      661 aatggcttca gagtgctttc gggcggcaaa tacggcggaa ataacggttt tgatcatgta
      721 tggcaggctg ccgatggtag tgtcgttttg attgtagaaa gtaagcagat taggaacggt
      781 acggtacagc tgaatccgaa tggtgcgggt ggatatacgc aaatgagtga ggattggatt
      841 aaacaagttg caaatagctt acctgatggt agtcctgcta aagctgctgt cttcaaagcg
      901 gagcgggaag gcaaattaaa aacggcaata gcaggcgttg atcgtcaaac aggtaaggcc
      961 gttattcttt ctgtcaaagt tccttctaaa accaatataa ggagataaca atggggcaca
     1021 atatgatgac cacccaaaaa tggtatgagc atattactaa tgtaatcata ggcaatactg
     1081 ctaatttcaa tagcggttgc cctgaagcta tagattatgt agatgaaaga aaaggcgttc
     1141 cgcttgcagc gatgagacat attttaatgt atactgaagc tgcggcttcc catgcctatc
     1201 tatttgaaca tgatcttaat aaattcaagc aatatgctta tgttgcagga aagctgggta
     1261 ttttgcggag tgtaaattct acagaccctg aacccttctt ctttccctgt gacatgctca
     1321 acattcaaaa tccgatgttt ctgatgctga tgagcgacag cccacagctg cgcgagtttc
     1381 tggtgcgcaa tatcgacaac atcgccaacg atacagaagc ctttataaac cgctacgacc
     1441 tcaaccgtca tatgatttac aatactctgc tgatggtgga gggtaagcag cttgatcggt
     1501 tgaaacaacg tagcgagaaa gtcttggcgc atcccacccc tagcaaatgg ctgcaaaagc
     1561 ggttgtacga ttaccgcttc ttcctcgctt tcgccgaaca ggatgccgag gcaatgaaag
     1621 ccgccttaga gccgcttttc gataaaaaaa ccgcgcgtat ggctgccaaa gaaacattgt
     1681 cctatttcga tttctacctg cagccgcaaa tcgttaccta tgccaaaatc gcatccatgc
     1741 acggtttcga tttgggcata gaccacgaaa tcgcaccgag ggatttgatt gtttacgatc
     1801 cgctgccagc agacgaatat caagacatct tcgattttat gaaacagtat gacttgtctt
     1861 acccgtatga atatctgcag gattggatag attactatac gttcaaaacc gataagctgg
     1921 tatttggtaa cgcgaagcga gagtgagccg taaaactctg agctcctgtt ttatagatta
     1981 caactttagg ccgtcttaaa gctgaaagat tttcgaaagc tataaattga agcccttcca
     2041 tagtacatag atctgtgttg tggcgaggct ttaccacgct gattgccgga gaagaactca
     2101 acctgctggc aaaacaaggc atgagatctt tgcaataaca tgagttgaga cctttgcaaa
     2161 aaagcccttc cccgacatcc gaaacccaaa cacaggattt cggctgtttt cgtaccaaat
     2221 acctcctaat tttacccaaa tatcccctta atcctccccg gatacccgat aatcaggcat
     2281 ccgtgctgcc ttttaggcgg cgcgggcgca cttagcctgt tggcggcctt caacag
//
LOCUS       DAWKOD010000208         2313 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000208 DAWKOD010000000
VERSION     DAWKOD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.208"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            31..510
                     /locus_tag="WJK73_001967"
     CDS             31..510
                     /locus_tag="WJK73_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ7558391.1"
                     /translation="MPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJK73_001968"
                     /gene_synonym="lytB"
     CDS             complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJK73_001968"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ7558392.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1583..2080)
                     /locus_tag="WJK73_001969"
     CDS             complement(1583..2080)
                     /locus_tag="WJK73_001969"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ7558393.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          536 a          717 c          558 g          502 t
ORIGIN      
        1 ctgcccgaaa tcatccgcga cctgctcgaa atgccgtctg aaaccgccat tgccgacatc
       61 acacgcactt attccgcaca ttacctcaat cccaacaacc gcaatatgac gctctttccc
      121 gatgccctgc cctgtctgga caagctcaaa gcacaaggat actggcttgc cgtcgccacg
      181 ggcaaagggc gggcgggctt ggacaacgcc atcagccaaa ccgccaccgg cggctattgg
      241 ctcgccaccg cctgcgcggg ggaatatccc tccaaaccct cgcccgaaat ggtattcgga
      301 ctctgcggcg aactgggact cgatccgaaa gaagcattgg tcgtcggcga tacggcgcac
      361 gacctgcata tggcggcaaa cgcaggcgcg gcggcagtcg gcgtggccac cggcgcacat
      421 tcgcgcgaac agctccttag cgcaccgcat ctcgccgtat tggacgactt gtccgaactg
      481 cccggttttc ttgcacaaca ttacgcctga ttggtttccg catcagacac tcgaaacaaa
      541 tgccgtctga agcctgttca gacggcattt gtgttgccca aacattcaac gtttgcacaa
      601 atcgggtttg gtttcgccct cgcggcgcag ctctttgggc aggacgaaca caatgctttc
      661 ttccgcaccc tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg ttgccagtac
      721 ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc
      781 ctcaaaccat gcgcgttgca ggtagcctgc attatccacc atatacgcat cgattccgcg
      841 cgatgccgcc acttcgcgca agcggttgct gttggacgaa ttgggcgaac cgaccacaat
      901 cacgatgtcg cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata
      961 gcagatatct tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat
     1021 gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg
     1081 gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg
     1141 cgcaagctgc cccatcgtgc cttcgacctc gacgtgcccc ttatgcccga tcatgatgat
     1201 ttcacagtct tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca
     1261 agtcgcatca aacacgcgga aaccgcgctc cgccgcttct tgctgcaccg ccttcgatac
     1321 gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa
     1381 caccgcacct ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg
     1441 cacataaatc ggcgcgccga actcttccaa agcacgttcg acaatactga ttgcccgatc
     1501 cacaccagcg cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagccc
     1561 ggtatttcgt tttcagacgg catcaatatt tttcttcttg ggttttacgg tggacgatgt
     1621 tgtccaacac cgccaacacc gcaccgacgc agataaagct gtcggcaata ttaaaggcgg
     1681 gataaaacca attttgccaa taaaacaata agaaatcgac gacatgaccg tgtatcaggc
     1741 gatcgatgac attgcctaac gcaccgccga taatcatcgc cgcacccgtt ttgccgaggg
     1801 ttgcaaactc atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc accgccagca
     1861 ccaaaaaaaa gtatttttgc cagccgccct gatcggcaag gaagctgaac gccgcacccg
     1921 ggttgtacac caaggtcaaa tcgaaaaagg aaggaatgac attgacgcgt tcccgatact
     1981 gaaacgacga cagcaccgcc cacttcgacc actggtccag cacgatggcg gcaagtgcca
     2041 atacccaata gcgcgtttta cttgaaacag atgaagacat atttttcaac agccggtaaa
     2101 agagtaccat tttacccgaa aacccccttt cctgtacccg aaacggcaaa tgccgtaatc
     2161 ttaaaacccg tcattcccga caacaccgta atctcgaaac ccgtcattcc cgcgcaggcg
     2221 ggaatccaga cctgtccgca cagaaactta tcggataaaa acagttgccc aaaccccgcg
     2281 ttctatagtg gattaaattc aaaccagtac ggc
//
LOCUS       DAWKOD010000209         2307 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000209 DAWKOD010000000
VERSION     DAWKOD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.209"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WJK73_001970"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WJK73_001970"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ7558394.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WJK73_001971"
     CDS             1627..2193
                     /locus_tag="WJK73_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7558395.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          564 c          663 g          552 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagtcc
      301 cgtctgtgcc ggcgatgggc agcgtatcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacagacctg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcacgccgga
      481 cggcagacgc tgcctgttcg gaaacggcgg gcagtttgcc gtcgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggctttga gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gatatgccga
      661 tgccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcgacc tgtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggttttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggcat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacactgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaaca agtcgaaagg ccgcaaaacc
     2221 ggttcgggtt ttgcggcttt tttatatggg ctgggcagcg tcaaggtttg ccgtttgccg
     2281 gataatgcgc ggcaaatgac cggcgtg
//
LOCUS       DAWKOD010000210         2286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000210 DAWKOD010000000
VERSION     DAWKOD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.210"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="WJK73_001972"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="WJK73_001972"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ7558396.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJK73_001973"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJK73_001973"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ7558397.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="WJK73_001974"
     CDS             complement(1884..2192)
                     /locus_tag="WJK73_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558398.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          468 a          542 c          730 g          546 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat gagatagggt tcgataacgt cttcgatggt gtctgttgat
      901 tcgccgatgg cagcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga ggaatttcct gtccatcacg tccagcccct gcacgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc ggcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agttgcagga gttgtgccga acggctgacg
     1201 atggtggcaa ggtcgcggtt ttcgtaaaac tcgaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgacaagcgt gaagggcggc
     1321 aggtcgattt taacggaacg ggcggcgggc ccttcgccta tcataatgtc gagccggtag
     1381 tcttcaagcg cggggtagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 ataaacaata cgtcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gccggcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttacccag cccgggcgga ccgaaaagca agacgtggtc gagtgcttcg
     1621 ccgcgctttt tggcggcttg gatgaaaatg gcgagctgtt ctttggcttt atgctgcccg
     1681 atatagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacaat gcgttgcggt tgagcggcag tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtct gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttacccg cgcgtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgt gggcagatgc ctgtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt gataggcgtt ggggtcgaag cggacttgct gcatcaccgc ctgcgccaac
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa taggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgatttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaact
//
LOCUS       DAWKOD010000211         2268 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000211 DAWKOD010000000
VERSION     DAWKOD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.211"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..574)
                     /locus_tag="WJK73_001975"
     CDS             complement(<1..574)
                     /locus_tag="WJK73_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ7558399.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWNEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WJK73_001976"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WJK73_001976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ7558400.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLSKAVVKLSDAPMYIDETPGLTALELRARARRLARQFNGKLGLI
                     VIDYLQLMSGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          547 a          571 c          617 g          533 t
ORIGIN      
        1 cgaagcgttt ggcgacttcg ttccagttga cgatttccca aaaacctttc aggtagttgg
       61 ggcggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggagttgctg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc cacgcccaac cggagccgaa cgtaccggca gcgcaagcgg
      241 agaatgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 caccggaagg tttgccttga cctttgggcg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgggc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag cttggcagcg cacatatttt acctgttttg
      661 aggaatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acaaatagga tgtaccatga acgattacgc agccatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg cttccgccgc actcaatgga ggcggaacaa tccgttttgg gcgggttgat
      841 gctggaaaat ccggcatggg acaggattgc cgatgtggtt tcgggagagg atttctaccg
      901 gcatgaacac cgcctgattt tccgttccat tgccaaatta atcaatgaga gccgtcccgc
      961 agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 attcgaatac ctgattacgc tggcgcaaaa caccccgtct gccgccaaca tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccattat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcctacaatc cgcaaggcag ggacgcgggg cagcttttag acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac ggccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacggggtt catcgacctc gacaaaaaaa cctcgggcct
     1381 tcagcccggc gacctgatta tcgttgccgg tcgtccgtct atgggtaaga ccgccttttc
     1441 tatcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggggcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgtctga gtaaagcagt
     1621 cgtcaagctt tccgatgcac ctatgtacat cgacgagacc ccgggtctga ctgcgctcga
     1681 actgcgcgcc cgtgcacgcc gtcttgcacg tcagttcaac gggaaattgg gattgatcgt
     1741 catcgactac ctgcaactga tgtcgggttc ggggcgttcc gacaaccgtg cttcggagct
     1801 gggagagatt tcacgttcgc ttaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ttgagccgta ccgtcgaaca gcgtacggac aaacgcccca tgatgtccga
     1921 ccttcgcgag tccggcgcaa tcgagcagga tgccgacctg attatgttca tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaagat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaa
//
LOCUS       DAWKOD010000212         2175 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000212 DAWKOD010000000
VERSION     DAWKOD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.212"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WJK73_001977"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WJK73_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ7558401.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(1928..2175)
                     /locus_tag="WJK73_001978"
                     /pseudo
     CDS             complement(1928..2175)
                     /locus_tag="WJK73_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346148.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          549 a          628 c          568 g          430 t
ORIGIN      
        1 gaaaggcaag gacttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatt atcgcccacg tcgaccacgg caaaaccaca ttagtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 ccttgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac tttggcggcg aagtggagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggat gcgcaagaag gtccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gataaaccgt ccgcccgtcc gagctgggtt atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 tttgagcggc tttgccaagc tggaagaaac cgacgagagc agcgatatgc gcccgctgtt
      661 cgacaccatc ttaaaacaca cgcccgcacc gagcggcagc gcggacgagc cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggcatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgagc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggcttg gaacgcgtac cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ccgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgtgc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc
     1801 ggtcgagttt atcgacgatg atgagctggt agaaatcacg ccgcaatcca tccgcctgcg
     1861 caagcgttac ctgagcgaat tggaacgccg ccgccatttc aaaaagctag attgatgttt
     1921 actggattta atgtttaaat gatactcgat gccgtctgaa aaaagttcag acggcatttt
     1981 ttattcggac gggctttgcg gcttcttgaa gctgtttcag acggcgtttt tcctacccaa
     2041 tcaagaaact gccgccattt ttccagcggc atatcgcccc gtcgcgtttc ggtatcgggt
     2101 tcggcttccc ggcagcatgt cgaacaatgc cgtttgaagg aatacgcctt ttgagtcctt
     2161 acaagcttag gaaga
//
LOCUS       DAWKOD010000213         2165 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000213 DAWKOD010000000
VERSION     DAWKOD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.213"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..814)
                     /gene="cysW"
                     /locus_tag="WJK73_001979"
     CDS             complement(<1..814)
                     /gene="cysW"
                     /locus_tag="WJK73_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ7558402.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIIT"
     gene            complement(1192..2028)
                     /gene="cysT"
                     /locus_tag="WJK73_001980"
     CDS             complement(1192..2028)
                     /gene="cysT"
                     /locus_tag="WJK73_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ7558403.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT          565 a          606 c          587 g          407 t
ORIGIN      
        1 tggtgatgat gttctgcacc gccagcgtcg ccagtgccaa aagtgccaat acgccggaga
       61 gggcgaatgc gccggtgaag ttgtattcgt tgtagaagat ttcgaccaaa agcgggacgg
      121 tgttggtttc gccgcgtatg tgtcccgata ccacgctgac cgcgccgaac tcgcccatcg
      181 cgcgggcgtt ggtgaggatg atgccgtaga gtaacgccca tttgatgttg ggcagggtaa
      241 cgcgccaaaa catctgccag ccgcttgcgc cgagtatcaa tgccgcctgt tcttcgctgt
      301 cgccctgagc ctgcatcagc gggatgattt cgcgcgcgac aaaggggaag gtaacgaaca
      361 gcgtcgccag aataatgccg gggatggcga agataatctg tatgccttgc gcttcgagcc
      421 agccacccaa tgccgtgtgc gcgccgaaca ataagacgaa cattaaaccg gccaccacgg
      481 gcgatacgga aaacggcaaa tcgagcaggg tagtcagcaa ctgcttgcct cggaagtcaa
      541 aacgggtcag cagccacgcc atcgccacac ccaatacagc attgacggga acgacaatca
      601 gcgcggtaat cagcgtcaat ttgatggcag accacgcttc gggatcgttt aaggatttta
      661 ggtacaaatc ccaaccgcct tttaaggctt cgtaaaacac ggcgacgagc ggcacgacca
      721 gcatcagcag cagaaagccc agcgcggcgg caatcagcaa cacgcgcagc cggcgcggtt
      781 cggtcaggtt gggattggcg gaatagggtt tcatggcggt ggtgttctct ggttggttta
      841 aaatgtttgg aggtcgtctg aaaagggatt gaggggtttc agacgacctt tttgttcatt
      901 aagtagtagg tcggatactt gtatccgaca aggttgctga atcgcaaatg tttgtcggat
      961 acaagtatcc gacctacgaa ttctgtgttt tggggagcgg attcgttttc agacgacctt
     1021 ttatctttta tgctaaaaga gaaataaaaa agtctagctg ccgtcattcc cgcgcaggcg
     1081 ggaatccacc acagggcaac aggaaaacag aaaataaata aggcagctga aattcaccaa
     1141 tggattcccg cctgcgtggg aatgacggta acaggtattt cagacgccgc ctcaaccctt
     1201 cgcgcccgaa cgcctgccca acgcccactg catcacgttc agcgcaaaca gaatcacaaa
     1261 cgaaaccagc agcataaaca acgccaccgc cgacgcgccc tgcacgtcga actgttccag
     1321 cttgcccgta ataatcagcg gtaggatttc ggaaaccatc ggaatgttgc ccgcgataaa
     1381 aatcaccgag ccgtattccc ccgttgcccg cgcgaacatc attcccgcgc cggtcaagag
     1441 tgccggcgtg atttccggca gcagcacgcg gcggaacgtc gtaaagcggt ttgcgcccaa
     1501 agtcgccgcc gcttcctcat attcgcccga taattcctcc aacaccggct gcacggcgcg
     1561 gacgataaag ggcaggctga cgacgaccag cgcaatccaa atgccgacgg gcgtaaacgc
     1621 gattttgatg cccaaaggct cgaaaaaacg gcctatccaa ccgttgggcg catacagggt
     1681 tgccaacgcg atacccgtaa ccgccgtcgg cagcgcaaac ggcaaatcga ccagcgcgtt
     1741 cgccagaccc ttgcccggga attcataacg caccaatacc cacgccacca gcgtgccgaa
     1801 cacgacattg gtcagcatcg cataaaacga catccgcaaa ctcagccaca ccgccgccaa
     1861 cacgttcggc tcggcaatcg tgttccaaaa gccgccccag ccgatttccg ccgccttcgc
     1921 cgccatcatc gcaaacggca agaccacaag cagcgacagg cacaatacgg tcagaccaag
     1981 gctgagtttg aagccgggca gtacgccggg cgttttgagc ggtaacataa aacaatgctg
     2041 aaaataagga aaaggaagga ctactttaac gatgccgtcc gaaaaacgga aagaatggaa
     2101 agtttggtgc aaagacgaat ttgttataaa gcggttggca gtttctcaag cgggcgcgat
     2161 gtttt
//
LOCUS       DAWKOD010000214         2142 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000214 DAWKOD010000000
VERSION     DAWKOD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.214"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..463
                     /locus_tag="WJK73_001981"
                     /pseudo
     CDS             30..463
                     /locus_tag="WJK73_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            559..1182
                     /gene="rsmG"
                     /locus_tag="WJK73_001982"
     CDS             559..1182
                     /gene="rsmG"
                     /locus_tag="WJK73_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ7558404.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1281..2054
                     /locus_tag="WJK73_001983"
     CDS             1281..2054
                     /locus_tag="WJK73_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ7558405.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          420 a          490 c          738 g          494 t
ORIGIN      
        1 gcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 ttgcgcttgc gttcggacgg gcggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgtggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      241 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      301 gtggattttc gagctgctgt ttggcagtgc cgccttgggc gggctgcgtg gggaggcggt
      361 gttggaatat gcgtttgccg tgtggctggt gtcgatgctg acactgccca aacgcttgac
      421 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggacggaa cgggggatgc
      481 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgttaacat tacgcctgaa
      541 catggacagg atagggatat ggaacgcaaa gaacgcctgc gtgcaggcat cgccgcattg
      601 ggtttggata tttccgcagc ggcgcaggac aggcttttgg cctatgtgga tttgttgaaa
      661 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat ggttgtccat
      721 catcttttgg acagcctgac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      781 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      841 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      901 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
      961 gccgacgtgg ttaccagccg tgcgtttgca gaactggcgg actttgtatc gtggacggcg
     1021 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc gcagggggaa
     1081 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccaggc
     1141 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt cagacggcat
     1201 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1261 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1321 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1381 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1441 aggcgagttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgaaatcgg
     1501 cggcggtacg cagcaaagag ggcggatacg gcgtgttggg tgcgaaccgc gcgctggccg
     1561 gcgcggaaat cgagctggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1621 aggcagtggc ggaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1681 tgacgcttaa cggcttggtg gcggcaggcg gcgtgattgt gccgatgttg tgcgaatatt
     1741 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1801 atcccgattt ggatatcacg ggcatcgtgc gtacgatgta cgacagccgc agcaggctag
     1861 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgctgttt gaaaccgtca
     1921 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttatg
     1981 atgcgcaggc aaagggtgcc aaggcgtatc ttgccttggc ggacgagctg atggcgaggg
     2041 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2101 tgcgttaata tggcggatta aaataaaaat acttatatcg tc
//
LOCUS       DAWKOD010000215         2134 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000215 DAWKOD010000000
VERSION     DAWKOD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.215"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WJK73_001984"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WJK73_001984"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ7558406.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1400..2089)
                     /locus_tag="WJK73_001985"
     CDS             complement(1400..2089)
                     /locus_tag="WJK73_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ7558407.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
BASE COUNT          452 a          626 c          553 g          503 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgaccg gataggttga ccagaatcac ttggtctttg cccattttcg gcgcgttttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acagtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgcgta cccaaaacct gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg tgatcggggg tgttcacgcc
      421 caaaccgcca gcctccacgc cgacgaggcg cacgttttct tcgccgatat acgggtggaa
      481 caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc tgcatctgcg ctttagcttc gttgccaatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgcgg ggccggcggc ggtaccgatg atgtagaacg tgtcgtccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcagcgtgc ggctgccgct
      721 ttctacaccg accacgttcg cacccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 gcgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc agatttgcgc gccgcccaga tgttcggaca accgcgcggc
     1021 gtggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aagccggaat
     1141 cagggtctcg gacacgaaca gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcagat ttcgtccttg tgcttcaaat tttcagacgg caatttatag
     1261 tggattaaca aaaaccggta cggcgttgcc ccgccttgcc gtactatttg tactgtctgc
     1321 ggcttcgccg ccttgtcctg atttttgtta atccactata tttttcaaac atgccgtccg
     1381 aacgggaaat tgtcggcaat tataactgtt tttcacacaa gcccgccatt tcaaaaaacg
     1441 cccgatacgc cgccgctttt ttcgccaaac tcaaaggata ggcacgcgaa gtcgaaggca
     1501 ggcgcgtcag cgtcaaatcc cgtccggcaa acggaaacgg cacggtttcg cccgttttcg
     1561 gcattttgat gccgccaccc tgaatatcga gcaggatttc cgtcgccttg ccgcctgtcg
     1621 tacaaatatg gcggcagtct ggtattttcg ccaacaccgc cgccaaatcg acggtttcga
     1681 ctacctttaa aaacttgtcg gacgcattgc cgtgttccct tgccgccttc aacacggtcg
     1741 gacaggacgc aatcccccgt tcgtgcaaaa acgccttgat tttctcggca tcaaacgctt
     1801 tttcagacaa cctttggaaa tgcgccgcat cattaaaaaa caccagcccg taaacgcgcc
     1861 acatatcgtt ttggaaattc ggataatgaa actgcatcgc gcgtttgtct tccttgggcg
     1921 gaaacgtccc catcatcatg accgcagcct tcggcggcaa cacggaatcg aagggatggg
     1981 tttcgatttc aagcagattt tcagacacgt tcaatcctcc aacaggcaaa acggcagaca
     2041 gccggttttt gcaccaaacc gccaaacacg gcaagccttc aaacagcatt atcacgccct
     2101 gccgttttcg gttacaatgc cgacatcaaa cgga
//
LOCUS       DAWKOD010000216         2048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000216 DAWKOD010000000
VERSION     DAWKOD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.216"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            185..1963
                     /locus_tag="WJK73_001986"
     CDS             185..1963
                     /locus_tag="WJK73_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ7558408.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     AGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          616 a          484 c          523 g          425 t
ORIGIN      
        1 cttaccggat aaaacagttt ccttagattc cacgtcccag attcccgcct tcgcgggaat
       61 gacgagattt taagttgggg gaatttatca gaaaaccccc aacccccaaa aaccgggcgg
      121 atgccgcacc atccgccccc aaaccccgat ttaaccattc aaacaaacca aaagaaaaaa
      181 caaaatgaac aaaatatacc gcatcatttg gaatagtgcc ctcaatgcct gggtcgccgt
      241 atccgagctc acacgcaacc acaccaaacg cgcctccgca accgtgaaga ccgccgtatt
      301 ggcgacactg ttgtttgcaa cggttcaggc gaatgctacc gatgaagatg aagaagaaga
      361 gttagaatcc gtacaacgct ctgtcgtagg gagcattcaa gccagtatgg aaggcagcgg
      421 cgaattggaa acgatatcat tatcaatgac taacgacagc aaggaatttg tagacccata
      481 catagtagtt accctcaaag ccggcgacaa cctgaaaatc aaacaaaaca ccaatgaaaa
      541 caccaatgcc agtagcttca cctactcgct gaaaaaagac ctcacaggcc tgatcaatgt
      601 tgaaactgaa aaattatcgt ttggcgcaaa cggcaagaaa gtcaacatca taagcgacac
      661 caaaggcttg aatttcgcga aagaaacggc tgggacgaac ggcgacacca cggttcatct
      721 gaacggtatc ggttcgactt tgaccgatac gcttgcgggt tcttctgctt ctcacgttga
      781 tgcgggtaac caaagtacac attacactcg tgcagcaagt attaaggatg tgttgaatgc
      841 gggttggaat attaagggtg ttaaaactgg ctcaacaact ggtcaatcag aaaatgtcga
      901 tttcgtccgc acttacgaca cagtcgagtt cttgagcgca gatacgaaaa caacgactgt
      961 taatgtggaa agcaaagaca acggcaagag aaccgaagtt aaaatcggtg cgaagacttc
     1021 tgttattaaa gaaaaagacg gtaagttggt tactggtaaa ggcaaaggcg agaatggttc
     1081 ttctacagac gaaggcgaag gcttagtgac tgcaaaagaa gtgattgatg cagtaaacaa
     1141 ggctggttgg agaatgaaaa caacaaccgc taatggtcaa acaggtcaag ctgacaagtt
     1201 tgaaaccgtt acatcaggca caaatgtaac ctttgctagt ggtaaaggta caactgcgac
     1261 tgtaagtaaa gatgatcaag gcaacatcac tgttatgtat gatgtaaatg tcggcgatgc
     1321 cctaaacgtc aatcagctgc aaaacagcgg ttggaatttg gattccaaag cggttgcagg
     1381 ttcttcgggc aaagtcatca gcggcaatgt ttcgccgagc aagggaaaga tggatgaaac
     1441 cgtcaacatt aatgccggca acaacatcga gattagccgc aacggtaaaa atatcgacat
     1501 cgccacttcg atggcgccgc agttttccag cgtttcgctc ggcgcggggg cagatgcgcc
     1561 cactttaagc gtggatgacg agggcgcgtt gaatgtcggc agcaaggatg ccaacaaacc
     1621 cgtccgcatt accaatgtcg ccccgggcgt taaagagggg gatgttacaa acgtcgcaca
     1681 acttaaaggc gtggcgcaaa acttgaacaa ccgcatcgac aatgtggacg gcaacgcgcg
     1741 tgcgggcatc gcccaagcga ttgcaaccgc aggtctggtt caggcgtatc tgcccggcaa
     1801 gagtatgatg gcgatcggcg gcggcactta tcgcggcgaa gccggttacg ccatcggcta
     1861 ctccagtatt tccgacggcg gaaattggat tatcaaaggc acggcttccg gcaattcgcg
     1921 cggccatttc ggtgcttccg catctgtcgg ttatcagtgg taagggcttt ttcgcctgtc
     1981 tgctgttggg acaggcggaa ggtttgaagg gaagggtggc gatttgccgc ctgagacctt
     2041 tgcaaaat
//
LOCUS       DAWKOD010000217         1946 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000217 DAWKOD010000000
VERSION     DAWKOD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.217"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            244..1701
                     /locus_tag="WJK73_001987"
     CDS             244..1701
                     /locus_tag="WJK73_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7558409.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          431 a          583 c          448 g          484 t
ORIGIN      
        1 ccgccctatc ggataagtcg cttgactgaa aaggctttac ccgcgccgta tcggaacacg
       61 tcctctaaaa tacaatccgt tgaattgaaa caaatataaa aacattcgac cgcaaaaacg
      121 gcagcgcatc gtttgacaaa gattgaaaat atcggttaaa aaccgatttt catacaaaaa
      181 acaccgctgc cgtccgcatc cgtttcagac ggtattgaga gaaaatcttt taggagaacc
      241 tttatgtccc ggcatcccgc ccgcaccgga gaaaaaacat tcttcggcca ccccttccag
      301 ctttccaccc tcttccatat cgaattgtgg gaacgttttt cattttacgg aatgcagggc
      361 atcctgctga tttacctcta ctacaccgcc gacaaaggcg gcttgggcat agacaaaacc
      421 ctcgccggcg gcattgtcgg cgcatacagc ggcagcgtgt acctgtccac cattttgggc
      481 gcgtggtttg ccgaccgcgt atggggtgcg gaaaagaccc tcttcctctc gggcatcgtc
      541 gtgatgctcg gacacatcgt ccttgccgcc gccccgggcc tgtacggcct tttaatcggg
      601 ctgatattca tcgcattggg cagcggcggt gtgaaatcta cggccagttc tatggtgggc
      661 gcattatacg aacaggacga aatgcgcccg ctgcgcgatg cgggattttc cattttctac
      721 atcgccatca atatcggcgg cttcctaggc ccgctgctga ccggcctgct gcaggaaaac
      781 atcggtttcc attatggttt cggcgcggcg gcggtcggta tggcattcgg cttgtggcgt
      841 tattccttgg gacgtaaaaa cctgccccac cccaccgtcc cccatccgct ttcaaaagga
      901 cagggcaaaa ctgcggccgc cgtcggcatc gccctcatcg ccgcacttgc aaccgccatc
      961 aaaaccgggc ttgtcaacct cgacaatttc tccggcatcc tcctatctac cgtcatcctt
     1021 gccgtcatcg tctattttac ccggctgctg accaaccccc gcgtcagttc cgacaacaag
     1081 cggcacatca tcgcctacat cccgcttttc ctgaccatct gtatgttttg ggcggtctgg
     1141 tttcagattt acaccgtggc aaccgtctat ttcgacgaaa ccgtcaaccg caccatcggt
     1201 tcgtttaccg tacccgtcgc ttggaaagat tctatgcaaa gcctgtgggt catcctgttt
     1261 tccggactta tggcggcaat gtggacaaaa atggggcgca aacagcccaa aaccccgctg
     1321 aaattcgcta tggcggtatt tgttaccggc gcgtcgtttt tgggattcgt cccctttatt
     1381 tccgccggca cgccgatgcc tattgcggtt ttcgcactga ttgtccttgc catcacgata
     1441 ggcgaactga tgatttcccc gattgcgctg tccatctcca ccaaaatcgc accgccttta
     1501 ttcaaaaccc aaatggtcgc ccttaatttc cttgcctttt cattaggctt cactttgggc
     1561 ggcgtattgt ttgaaaaagg ctatcaggcg ggcgacgaaa tcggcttcta tcggctgctg
     1621 ttctacatcg gcgcagccac aggcttcctg ctgctcctgc tcgtccccaa attgaacaaa
     1681 atgctcgaag gcacagacta agtcccgccc cgatgccgtc taaacccttc agacggcatt
     1741 tttccgcata atgaaaccaa accgtttcca cccgacagga caggctcccc ccccaaccgg
     1801 aaggcagcct gccgattgtc atttaaatat ctgactgatt ccgtctgttt gcaagggaaa
     1861 tagccgttta tttccgcttg ggcggaaacc ggttctattg agtaaaaggc attttatccg
     1921 actaaattag tgctgcatca acggaa
//
LOCUS       DAWKOD010000218         1829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000218 DAWKOD010000000
VERSION     DAWKOD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.218"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            325..1770
                     /gene="zwf"
                     /locus_tag="WJK73_001988"
     CDS             325..1770
                     /gene="zwf"
                     /locus_tag="WJK73_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ7558410.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          505 a          416 c          481 g          427 t
ORIGIN      
        1 aaaggtatac cgtctgaaag tgggaagcgg gtgttattat agtacaaaat ttgtaatttt
       61 gttacaaaaa aaatcagaca tcgaaaagaa atttgattta aatcgcagaa tctttcgatt
      121 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      181 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      241 ttgtttccga tattgcgatg cagcgtgatg gggaaaacag gtttcagacg gcatcgaaaa
      301 attctttcct gacgagataa tgcgatgagt acacaaacaa attttgattt ggtgttgttc
      361 ggtgcgaccg gcgatttggc aatgcgcaaa cttttgccct gcctgtatca ggcgcacgtg
      421 gccggtttgc ttcatcccga aggtcgtatt ttgggtgtga gccgcagcga attggatacc
      481 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      541 gatgaggctt gggcatcgtt tgtcgaacgt ctcgcatatc tcaaagtcga tgttacgcag
      601 ccggacgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      661 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaaa catgtgaaaa ccttgccaaa
      721 atcggtttga acgccgacaa cgtgcgtgtg gttttggaaa aaccgttggg tacggatttg
      781 gcttcttccc aacaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatctac
      841 cgtatcgacc attatttagg taaagaaagc ctgcaaaacc tgctggcctt gcgttttgcc
      901 aatgtgatgt tcgagccgct ttggaacaat aaatacatcg aaagcgtgca gcttaccatc
      961 gccgagcagt tgggcgtgga agaacgcggc gagttttacg acattaccgg cgcgttgcgc
     1021 gatatggtgc aaaaccactt gatgcaaatg ctgtgcatga ctgcgatgga agcccccgcc
     1081 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1141 ctgaccgtcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc cgccaaaggc
     1201 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1261 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1321 ggcaagcgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1381 catatttttg aaggcagtcg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1441 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1501 gtgccgctgg caaccgattt gggcaaagca ttggaaagcc gccgcgcgga agcttacgag
     1561 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1621 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1681 ccgcacggct acggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1741 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1801 tgaaatgccg tctgaacgta agtaaagca
//
LOCUS       DAWKOD010000219         1823 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000219 DAWKOD010000000
VERSION     DAWKOD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.219"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..234
                     /locus_tag="WJK73_001989"
     CDS             <1..234
                     /locus_tag="WJK73_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558411.1"
                     /translation="KKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSV
                     GIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK"
     gene            298..1431
                     /locus_tag="WJK73_001990"
     CDS             298..1431
                     /locus_tag="WJK73_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558412.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDKSGGGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVDYQNMRELMPRGMKANSLVVGYDADGLPQK
                     IYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHTQQTWFLDGGRI
                     VREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1518..1793)
                     /locus_tag="WJK73_001991"
     CDS             complement(1518..1793)
                     /locus_tag="WJK73_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558413.1"
                     /translation="MNESEVVLTPEQIQTLYGYAFRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
BASE COUNT          513 a          356 c          462 g          492 t
ORIGIN      
        1 aaaaaatatc cttatccgat ggatatacca agggatattg tgattggaat cagtaccatt
       61 attgattttt taatggttcc aaattgggag cttttcgaaa ttaaagcttc cccttggcta
      121 cctgatagcg tgggaattca tgaacgttat gaaagattca caacaatgct ccgttatatt
      181 tttaccgaga aagacatagt caacgtgcga tttgattatt acaacaaaaa atagcaaacc
      241 ccggcaatca aagctcgtct gatataatcg atatattctg ttaaacgaaa aataaatatg
      301 tttaaaaaat tcaaaccggt attgttgtca ttttttgcac ttgtatttgc cttttggctg
      361 ggaacgggta ttgcctatga gattaatccg cgttggtttt tgagcgatac ggcaactgaa
      421 gtacctgaaa atccgaatgc ttttgtggcg aaacttgccc gcctgttccg aaatgccgac
      481 agggcggttg tcatcgtgaa ggaatcgatg aggacggagg aaagtcttgc cggagctgtg
      541 gatgacggtc cgttgcagtc ggagaaggat tatctcgcgc tcgctgtccg gctcagtcgt
      601 ttgaaagaaa aggcgaaatg gtttcacgta acggagcagg aacatgggga agaggtttgg
      661 ctggattacc atatcggcga gggcggtttg gttgcggttt cgcttttgca acgctcgccg
      721 gaagcgtttg ttaatgccga atatctgtat cggaacgatc gtccgttttc tgtaaatgtg
      781 tacggcggaa cggttcacgg agaaaattat gaaacgacag gagaatatcg ggttgtttgg
      841 caaccggacg gttcggtatt tgataagtcg gggggcggga aaatcgggga agatgtttat
      901 gagcattgtc tcgggtgtta tcagatggcc caggtatatt tggcgaaata ccgggatgtt
      961 gcgaatgacg agcagaaggt ttgggacttc cgcgaagaga gcaaccggat tgcgtcggac
     1021 tctcgcgatt atgtggatta tcagaatatg cgggaattga tgccccgagg gatgaaggcg
     1081 aacagtcttg tggtcggcta tgatgcggac ggtctgccgc agaaaatcta ttggagtttc
     1141 gacaatggga aaaaacgcca gagtttcgaa tattatttga aaaacggaaa tctttttatt
     1201 gcacaatctt cgacggtagc attgaaagcg gatggcgtaa cggcagatat acagacctat
     1261 catacgcaac agacgtggtt tttggatggc gggcggattg tccgcgaaga gaaacaggga
     1321 gacagactgc ctgattttcc tttgaacttg gaagatttgg aaaaagaggt gagccgttat
     1381 gcagaggctg cggcgagacg ttcgggcggc aggcgcgacc tttctcactg aacgcaaatg
     1441 ccgtctgaaa ccggttttca gacggcattg gagagtcaaa cgctttttgc aatgccgtat
     1501 atgccgaatc cgattcgcta caaattgaaa accacaatcc aaaaaacgga aatacacatt
     1561 aaacggatct ttttaccgac cgtatcatcc cataggtttt ccacaccttt tttcatccat
     1621 aaattcaaac catttaagtt tgcatcctta ccgacaattg cggcagcatt atccgcataa
     1681 cggtcaccca aattagccaa ataacgccaa ccgccatagg tatcgccacg gaaagcataa
     1741 ccatacaagg tttggatttg ttctggtgtt aaaacaactt cactctcatt cataataaga
     1801 tcctttaaat tattttttac ttg
//
LOCUS       DAWKOD010000220         1810 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000220 DAWKOD010000000
VERSION     DAWKOD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.220"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..1152
                     /locus_tag="WJK73_001992"
                     /pseudo
     CDS             1..1152
                     /locus_tag="WJK73_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1197..1487
                     /locus_tag="WJK73_001993"
     CDS             1197..1487
                     /locus_tag="WJK73_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558414.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1492..1689
                     /locus_tag="WJK73_001994"
     CDS             1492..1689
                     /locus_tag="WJK73_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558415.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          552 a          427 c          394 g          437 t
ORIGIN      
        1 aaacctgtta ggcgttcctc ttcggcgcgg tgtatcagat tcggctttca ttgatcaaat
       61 tagcttttca tttcatgaaa aacccttttt cgataaatac ggcgttcgtg taagtctttt
      121 ggaagacgaa gattttattc gcgccgcgtc aatgctcgcc gaagaagttt tcggtttcgg
      181 tatctacaaa gaatccaaag gctcgggcgg gcgtttttat gagcgctgtt ggttgatggg
      241 ttcggaagac gccctatacg gtcgcgtcca ttttggcgga caaaacaata ccattctttt
      301 cgaactgacc ggcaccggtt gcggcgtcgc aaaagaaggc tgggaatcac gacttttcgc
      361 attcctgact aatgcaatcc gcccaaaaat cacacgcgtt gacatcgcaa aagacttttt
      421 caacggcgaa tacagcccga accaagcccg tgaagaccga aataaaggta tgtttacctg
      481 tcatcacgtc aaaccaaaag gcgaatgttt ggggtcagat tgggaagaag acgatgaagc
      541 caaaatgacc aaaggcaaga cctatggtat cggctcccgt gaatcgtcca aatatgtccg
      601 cgtctatgaa aaaggcaagc agttgggcga taaaacaagc acatggacgc gctttgaaat
      661 tgaattcaaa gcaaaagaca tcgttatccc ttttgaagtt ttgcagaatc cgggcgaata
      721 tttcggcggc gcatatccga tttgcgaacg attcgtccaa aaggcaacgc gcatacacgc
      781 ggttaaggaa gataaggtca tttcagccga ccgttacctt gaatgggtaa aaaacagttc
      841 ggacgtgcgg caaacggtct gaaattcatt tttcccgaat tggacaaagc caaactgttt
      901 gaactgattg agccgagtca tcacaagctg cccaagtctt tgactcccga agcctacgac
      961 tgcgcctttt tgaaagctca agccattcat gaacagcccg cattcaaacc gtacaaagac
     1021 ccttactata tgtacgaata ttacgagaat cttgaaaaac agcttgaaca gcaaaaacac
     1081 gtcaacaatg aagaaagcta taacaacttc atttacgaca aattcgcaag actaccgatt
     1141 tcatgggctt aaagtgtctg cccgaaagac gtttaatcac acaaggaaac caaaaaatga
     1201 acatccaact tcaaggccac atcgtcggcg ttaaaaaatt caacggacaa atcgaaggca
     1261 agagcttcga ctattgccgc ctgattgtcg ccacaccctt agacagctcc caaggcaacg
     1321 cattgggcag ctctactact gaatacgatt tcggcggctc tgccaatttc gagcagttcc
     1381 gaaacgccca atttcccatc gaagcaaacc tgaacgtgga aatcgttact acgggcaaaa
     1441 cccaaaaact gaaagtcatc ggttttcaac tcgtgaagaa aggctgattg aatgcagaaa
     1501 gtctatgttg tccagtccgt atcaacaggg gactttctgt atctctctcc tgaaacgggc
     1561 gacatcggac ataccaaatt aatcaccaat gccgattatt tctacgactt cgaagaagcg
     1621 attaacgcag gtttggaaga aatcggcaac caatacgaat ttgtcgtatt cggatttttg
     1681 aaagactgat tttcggatgt tcggcggtcg tctgaaaaac gctccatcca ttaccgccaa
     1741 acactttttg aaggaaaata tcatgaaatt tattaacacc tgccgtaaat acggcgcaaa
     1801 actggctgtt
//
LOCUS       DAWKOD010000221         1809 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000221 DAWKOD010000000
VERSION     DAWKOD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.221"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(188..844)
                     /locus_tag="WJK73_001995"
     CDS             complement(188..844)
                     /locus_tag="WJK73_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ7558416.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LLILFLLIFRLCWGIWGSDTARFSHFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKQKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            909..1799
                     /locus_tag="WJK73_001996"
     CDS             909..1799
                     /locus_tag="WJK73_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ7558417.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAVFPEIMLDTADGLTKRQD
                     INAWVVGCGLGTGRAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          398 a          426 c          591 g          394 t
ORIGIN      
        1 aatcggttct gtactatccg tactgtctgc ggcttcgtct ccttgtcctg atttttgtta
       61 atccactata aaacggtttg ggacggaaaa acggcctgaa gccgtttcgg gcattcagac
      121 cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag ggcttcagac ggcattgatg
      181 tcgggtttca ggacaggagc aggatggcgg ctgcggcaag cgcggcaacc gataatgcgg
      241 cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt gccttcgatg tatttgaagc
      301 cggttatcat cgggaggacg aggtttttct gtttgaacac gcggtatgcg gcgacagcgg
      361 cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa gttgaggtgg attttccgca
      421 taaggctgcc tgtatgttca gaaaccaaat ggttgaggta gccgttggtg ctgaaggtgt
      481 tttcatcggc ggcaaaaagc cccgtgccga cttgaaatga gacggcggcc aaaagcgcga
      541 cgaccatcag tgcgcccaag gggttgtgtc cgggctggat atgttcggga ataccgtttt
      601 tcagataatc cctgatacct gaccatccgc ggacgaaatg ggaaaaacgg gcggtatcac
      661 tgccccaaat gccccagcag aggcggaata tgagcaggaa aaggataagc agcccgacgc
      721 gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct ataccacata aagggtaggg
      781 atgcggcaag cagccaatgg aaaaggcggg tggggaagtc ccagactttg gttttgtttt
      841 tcataatcgg tttccggcgg tagaatcggt ttgttttcga gccttaattt taaccgattg
      901 gaggggaaat gtttcccgtt ttccatcttt caggcgaaag ccgccgccgg atgcttcaga
      961 cggcattgcg ttttccctat gttttcaaag cccgtgcgga agattcgcac aaagggactt
     1021 tcggcacgct cgccgtagtc ggcggatcgg cagggatgag cggcgcgccc gtattggcgg
     1081 catcggcggc gatgtatctc ggctgcggca aggtgtgggc gggtttcaat caggatgcgc
     1141 ttccgtttgc cgtcattgcc gtttttcccg aaattatgtt ggacacggca gacggtttga
     1201 ccaagcgtca agatataaac gcctgggttg tcggttgcgg cttgggtaca ggtagggcgg
     1261 cggtcggaac gcttgccgga attttggcgg aacacacgga caagcccgtc cttttggatg
     1321 cggacgcgct gaacatatta tcaaccgatg ccgaaacccg aaatctggcg cgcgggtgta
     1381 aaaacctgat tttaacgcca caccccgccg aagccgcgcg cctgcttgga acgacggttg
     1441 cacaggttca ggcggatcgg acggcggcag tgaggaagat aggggcaatt ttcggcgcaa
     1501 ccgtggtttt aaaggggcac aaaacattgg ttgctgcgtc cgatacggaa atctatgtca
     1561 acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg cgacgtattg ggcggcatca
     1621 tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc cgcctgcgcg ggcgcgtggc
     1681 tgcacggcgc ggcggcggat gtcataaaag aatcggcagg cattgcggcg gggctgttgg
     1741 ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg gataactgaa agtatgtaag
     1801 aagatatag
//
LOCUS       DAWKOD010000222         1798 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000222 DAWKOD010000000
VERSION     DAWKOD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.222"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            318..1484
                     /locus_tag="WJK73_001997"
     CDS             318..1484
                     /locus_tag="WJK73_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7558418.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     PNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQRPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLIGSATSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIERGK
                     KGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          460 a          416 c          457 g          465 t
ORIGIN      
        1 gtttttgtta atccactata aagtgccgcg tgtgtttttt tatggcgttt taaaaagccg
       61 agactgcatc cgggcagcag cgcatcggcc cgcacgaggt ctgcgcttga attgtgttgt
      121 agaaacacaa cgttttttga aaaataagct attgttttat atcaaaatat aatcattttt
      181 aaaataaagg ttgcggcatt tatcagatat ttgttctgaa aaatggtttt ttgcgggggg
      241 gggggtataa ttgatgacat atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt
      301 tacaaaagga agccgatatg cgaaaaaaac ttaccgccct cgtattgtcc gcactgccgc
      361 ttgcggccgt tgccgatgtc agcctgtacg gcgaaatcaa agccggcgtg gaaggcagga
      421 acatccagct gcagttgacc gaaccgctcc caaatattca acctcaggtt actaagcgca
      481 aaagccgcat caggacgaaa atcagcgatt tcggctcgtt tatcggcttt aaggggagtg
      541 aggatttggg cgaagggctg aaggctgttt ggcagcttga gcaagacgta tccgttgccg
      601 gcggcggcgc gtcccagtgg ggcaacaggg aatcctttat cggcttggca ggcgaattcg
      661 gcacgctgcg cgccggtcgc gttgcaaatc agtttgacga tgccagccaa gccattgatc
      721 cttgggacag caataatgat gtggcttcgc aattgggtat tttcaaacgc cacgacgata
      781 tgtcggtttc cgtacgctac gattcccccg aattttccgg ttttagcggc agcgtccaat
      841 tcgttccggc ccaaaacagc aagtccgcct atacgccggc tcattttgtt cagaataagc
      901 aaaatcagcg gcctactctc gttccggctg ttgtcggcaa gccggggtcg gatgtgtatt
      961 atgccggtct gaattacaaa aatggcggtt ttgccgggaa ctatgccttt aaatacgcga
     1021 aacacgccaa tgtgggccgt gatgcttttg agttgttctt gatcggcagc gcgacgagtg
     1081 atgaagccaa aggtaccgat cccttgaaaa accatcaggt acaccgcctg acgggcggct
     1141 atgaggaagg cggcttgaat ctcgccttgg cggcccagtt ggatttgtct gaaaatggcg
     1201 acaaagccaa aaccaaaaac agtacgaccg aaattgccgc gactgcttcc taccgcttcg
     1261 gtaatgcagt tccacgcatc agctatgccc atggtttcga cttgatcgaa cgcggtaaaa
     1321 aaggcgaaaa taccagctac gatcaaatca tcgccggcgt tgattatgat ttttccaaac
     1381 gcacttccgc catcgtgtct ggcgcttggc tgaaacgcaa taccggcatc ggcaactaca
     1441 ctcaaattaa tgccgcctcc gtcggtttgc gccacaaatt ctaaatatcg gggcggtgaa
     1501 gcggatagct ttgtttttga cggcttgcat tcattcttcg attgcaatct gactgccaat
     1561 ctgcttcagc cccaaacaaa aatccggata cggaagaaaa acggcaatta aagacagcaa
     1621 ataccgtctg aaagattttc agacggtatt tcgcattttc ggtttggttt gcacatatag
     1681 tgggggactg ttagtttttg cacaaggaac aaatagagta aaaaaaacgc tgaaatctcg
     1741 gaaagacgtg gatttcggcg tttttttgca tccggaaaag ttacgccagc tttttcac
//
LOCUS       DAWKOD010000223         1733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000223 DAWKOD010000000
VERSION     DAWKOD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.223"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            356..1660
                     /locus_tag="WJK73_001998"
     CDS             356..1660
                     /locus_tag="WJK73_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ7558419.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          432 c          510 g          435 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgtgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taa
//
LOCUS       DAWKOD010000224         1710 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000224 DAWKOD010000000
VERSION     DAWKOD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.224"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            61..1434
                     /locus_tag="WJK73_001999"
     CDS             61..1434
                     /locus_tag="WJK73_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7558420.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1614..>1710
                     /locus_tag="WJK73_002000"
     CDS             1614..>1710
                     /locus_tag="WJK73_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ7558421.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          441 a          560 c          405 g          304 t
ORIGIN      
        1 tccccatcct atcggtttcc cgcttcagac ggcataaggt ctgaaagaaa gactacaatt
       61 atgagtaatc cattttcctc tttaggtttg ggtacggaac tcgtttccgc actgaccgcg
      121 caaggttacg aaaacccgac gcccatccaa gccgccgcca ttccaaaagc actcgccggt
      181 catgatttac tagccgccgc ccaaaccggc acgggcaaaa ccgccgcctt tatgctgccc
      241 tcgctcgaac gcctcaaacg ttacgccacc gccagcacct cgcccgcgat gcaccccgtg
      301 cgtatgctcg tcctcacccc cacgcgcgaa cttgccgacc aaatcgacca aaacgtgcag
      361 tcctacatca aaaacctgcc gcttcgccac accgtcttgt tcggcggtat gaatatggac
      421 aaacagaccg ccgatttgcg tgccggctgc gaaatcgtcg tcgccaccgt cggacggctg
      481 ctcgaccacg tcaaacaaaa aaacatcagt ttgaacaaag tcgaaatcgt cgttctggac
      541 gaagcagacc gcatgttgga tatggggttt atcgacgaca tccgcaaaat catgcagatg
      601 ctgcccaaac aacgccaaac cctgctcttt tccgctacct tctccgcacc gatacgcaaa
      661 ctggcgcaag acttcatgaa cgcgcccgaa accgtcgaag tcgccgcgca aaacaccacc
      721 aacgccaacg tcgaacaaca catcatcgcc gtcgatacca ttcagaagcg caacctgctc
      781 gaacggctga ttgtcgattt gcatatgaac caggtcatcg tgttctgcaa aaccaaacaa
      841 agcgtcgacc gcgtaacgcg cgaactggtg cgccgcaacc tgtccgcaca ggcgatacac
      901 ggcgaccgtt cccaacaaag ccggctcgaa acactcaacg ccttcaaaga cggcagcctg
      961 cgcgtcctcg tcgccaccga catcgccgcg cgcgggctgg acattgccga actgcccttc
     1021 gtcatcaatt acgaaatgcc cgcccagccc gaagactacg tccaccgcat cgggcgcacg
     1081 gggcgcgcgg gcgcggacgg cgtggcgatt tccctgatgg acgaatccga acagaaaatg
     1141 tttgaatcca ttaaagagct gaccggcaac aagctgctca tcgagcgcat cgagggcttc
     1201 gagccgcaat ggtgggaaca gggcggcgca aaaccggaaa aaaccgaaat gcgcgaaccg
     1261 agacaacgca accgctacga atccgccagg gctcagcgcg aaaaaaatac ccgaccggaa
     1321 accacggcaa acgatgcggg cgcggcttgc ggaaaaattg ccggacgcag ccgccgaagc
     1381 cgtcgggaac accggacgtg cgccctgctc caaccgcgtt acggcgtaaa ataaccctga
     1441 aaatcaaatg ccgtctgaac atttcccgtt tcagacggca tttttcaaac cggactgacg
     1501 cattgggagc aaccgcccgc accggataaa tttcttccgc aaacagtttc agacggcatt
     1561 tgctgcctgt acaatatcat ttccttttcc actcaaacca tcaggatttc ccaatgtcca
     1621 cccaattcga tgtcgccgta atcggcgcag gtccggcagg ctcggtcgca tccgccctgc
     1681 tccgcaaaaa aggttatagt ggattaaatt
//
LOCUS       DAWKOD010000225         1700 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000225 DAWKOD010000000
VERSION     DAWKOD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.225"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(96..758)
                     /locus_tag="WJK73_002001"
     CDS             complement(96..758)
                     /locus_tag="WJK73_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7558422.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(771..1598)
                     /locus_tag="WJK73_002002"
     CDS             complement(771..1598)
                     /locus_tag="WJK73_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ7558423.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRTFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSATCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          327 a          449 c          516 g          408 t
ORIGIN      
        1 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgctgta ctggtttaga
       61 tttaatccac tatatgtttg tggggcggat atttttcagg gctgtatttt gtccagacat
      121 tcgagcagat cgagcggcgt tcggatgtgg aaatccgcct gccatgagtc ggtatcgtct
      181 tcatctgaga tgtagcccca ttcggcgagg acggttttca tgccggcgtt gcggcctgct
      241 tggatgtcgc gttcggcatc accgacgtag agtgtgtgtt gcgggtcggc gtggatttgt
      301 ccgcacgcat acagcatggg tttgatgctg ggcttgggtt cgccgcaggt gtcgccgctg
      361 acgacgacgg cgggtgggat gatgaagccg agtttgggga cgagtttgtc ggtgaagcgc
      421 atgggtttgt tggtgatgat gccccatttg atgccgcgtt ggttgagttc ggcaatgagt
      481 tcgttcacgc cgtcaaagag ctcggtgtct tgggcgtagc ggcggtcgta ttcctccaga
      541 tattcggtgc gccatcgggc atagtcgggg tggtcggggg tgatgcctgc gccgagtttg
      601 atcagtcctg ccgcgccgtg gctggcttgg gtgcggatct cgtccatgct ttttgcaggt
      661 agtccgtggc gagcgagcag ggtgttgagt gcgcctccga ggtcgagggc ggtgtcggca
      721 agtgtgccgt cgaggtcgaa gagtacggcg tgtatcatgt gtgttccttt tcagacggta
      781 tttttgttct gtttcaaacc ctgctcgaaa tcttccagca tatccaattc aaagcggctg
      841 aagcccgctt tttcgcgcgc ttcgatgttc acatagccgc ggaagataaa catatcgtaa
      901 cgggcaatca gactgcggaa cagggtaatc ggttccaaac cgcgttcgcg gcagaggtgt
      961 tgataccaat ggttgccgat ggcgacatga cccacttcgt cgcggtaaat gatgtccaac
     1021 acgccgcagg ttgccgaatc gccgcgctgc gccaccttcg cgcgtatccc cggcgtaacg
     1081 tccagcccgc gcgcttccaa cacgcgcggc accaaagcca tacgcaacag tgggtcgtag
     1141 gcggttttgt acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc gtaatcgaag
     1201 ccgaaagtgc gcaatctgtc gcgcatcagg cggaaatggt acacctcttc cttcgccact
     1261 ttcacccagt cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa
     1321 gccagattga cggcgttgaa ttcgatatgc gcaatcgcgt gcagcatcgc cgcatagcct
     1381 tctgccgtgt tcattttgcg cggggtcagt tgcgacggcg cgaccaaaac aggcttgtcc
     1441 ggccgtcccg cgcgggggaa gtccgccggt ggcgcgtttg cttccgcccc gtccgcattt
     1501 tgaacggcgg caaacgcctc atccgtcagc cgtccttttt catctgggtc gcccgaaagc
     1561 agggcgcgtt ccagcaaagc ataaatatcg ggtttcattt caagtccgcc gtgttcggaa
     1621 aacaaatatt atagcgttta aaaaaaacaa gatgaggcat ataatctccg cgattcggca
     1681 ttccgcgccc aaaccgtcaa
//
LOCUS       DAWKOD010000226         1678 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000226 DAWKOD010000000
VERSION     DAWKOD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.226"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(77..508)
                     /locus_tag="WJK73_002003"
     CDS             complement(77..508)
                     /locus_tag="WJK73_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558424.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTSCSLHET"
     gene            complement(524..>1678)
                     /locus_tag="WJK73_002004"
     CDS             complement(524..>1678)
                     /locus_tag="WJK73_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7558425.1"
                     /translation="AIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSG
                     VVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVA
                     DKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYT
                     QADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNT
                     PITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQ
                     TDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGY
                     SKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          463 a          313 c          283 g          619 t
ORIGIN      
        1 ttgagctttt cagacggcat tgagccgtaa atcatggaac gcgtgcgtgc tggagcacac
       61 accttacgca tggattttag gtttcatgca ggctacagct tgtttgagaa tatgtttttt
      121 gtatttcttt acactttttt tgatattcag ggtgagctat taagaaatca gatattgcaa
      181 tattaaagta atgatatgct aaattaatag gtataattaa ttcttcagga ttgaaatcat
      241 ctccaactga aaaataaata gaaggagctt tatcaatata ctcaaatcca tttacctctt
      301 cataatctcc aatccatctg gtagcatcta ttttttcata ttgagcacct attactccaa
      361 aatgtccggt agttgtaata tttttaaaac actctaaaag cgtatcttta gtttcaaatt
      421 ggtaaattgt ttcaaaaaaa atcgaaataa caaaatggtt gttatttata gattttttat
      481 ttaagtttag aaaaatatta tttttcataa tttttccttt aaattattct gggtgaatgt
      541 ttgtaattct tcctgtattt acatcaaaat atgatctaaa tttgattccg tcaaaggttt
      601 ctgagaattg aatgacattt tttctttccg atattgattt agttctttca ttttgagcaa
      661 ttttagaggc ttttgaatat ccttgtgaag cagcattttg agccatttga agtattttat
      721 catcagaaaa ttttttagga ttataaacag tttttatact tgaaatttcc ttaaatccac
      781 catcaggttt acctgtcctg tctagtgtag gaatctcata tttaattcgg gtaatgcctt
      841 caatatcagt ttgggtttca gattttacgc gtcctcctcg tgaatttagt tctgccataa
      901 aattggtgcg gttatgggct cctttaatgc cctgtttttg actaaaacca tcagcatttg
      961 ccagatgtct ttttaggttt gtatcgaaac tgatgccttc cgggtatttg acattagtaa
     1021 taggtgtatt ttgttttaca aaccctcttg gtattggaat gaatctagga ttaactcctg
     1081 aaataagtgt ataaccttgg tcggacatcc attcggataa aggctttgta ttgcccgatt
     1141 gaagccaaga tttagcagca gtattcaaat gataagactg taaagccaaa tctgcttggg
     1201 tgtaagattt gctgaataat ttataccatt catcatcttt accaattaga cctgcttccc
     1261 aagatgaatg aaggcttcta ctatcgatca aatgttgttt tctgattgtt ctacattcag
     1321 tactacggga tatatccgta catattgcaa tcgaagcagc aagtctttta tcagcaacat
     1381 tttgatactt ttttacagta ttttttctgc acagttgagg attattctgt ttggcgcatg
     1441 cagtcatttc gttatcaaat tctctaccct ctttgtcgct aagctgatta tttttcaccg
     1501 ctacctcagc cgcattcgcc gccgcattta catcgccgcc gaccacaccg cttaccgtac
     1561 cggcaaccag tttgctgtat gccaaaatct gttcgcgttc tttagctgtc aaagtgtcag
     1621 gatttttgcc gtttgtcaaa gcctccccga ctatctcgcc cacagccgca cctatcgc
//
LOCUS       DAWKOD010000227         1655 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000227 DAWKOD010000000
VERSION     DAWKOD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.227"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..602
                     /locus_tag="WJK73_002005"
     CDS             186..602
                     /locus_tag="WJK73_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ7558426.1"
                     /translation="MHLRRILPPTAEALMRSRYSAYVLHLIDYIIATTVPAQQAFLDA
                     AELMQWSIETEWLGLNVIAHRNFGKQHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQW
                     YFIDPTVPHPAMKQPCICGSGKKFKACCGKYLKPVA"
     gene            complement(820..1614)
                     /locus_tag="WJK73_002006"
     CDS             complement(820..1614)
                     /locus_tag="WJK73_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ7558427.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          420 a          489 c          394 g          352 t
ORIGIN      
        1 gtttgcgcct aaaaggcggc ccggatgcct gattatcggg tatccgggca ggattaaggg
       61 ggtattgggg taaaattagg aggtatttgg ggcggaaata gctgaaaacc tgtgtttggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtctc gggctatgcc ggctgctgcc
      181 gcccattgca ccttcgccga atcctgccgc ccaccgccga agccctcatg cgttcgcggt
      241 acagcgcgta tgtgctgcac cttatcgact acatcatcgc tactaccgtt ccggcacaac
      301 aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa tggctgggct
      361 tgaatgtcat cgcacaccgc aacttcggca aacagcacgc ccaagtcgaa tttgaagcct
      421 acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc gtcaacatcg
      481 gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa caaccctgca
      541 tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa cctgtcgcat
      601 aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaaa ccattcatac
      661 caagggtaag tatgaatggt caatacattg cgggaaaacg tcttacttgc tgcactgccg
      721 aaaagggaga aacggcagcg gtaatcagcg gaaaggattg tacccgaatt aatgttaaga
      781 aacgttaatc gtgaaatata ttaacaaacc tgttgaagcc tattggtttt cccgtatcca
      841 cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc gtttgaacac
      901 gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc
      961 gaaaatcaac gcgccggcgg cataatccca cagcttctgc ccgccgtgga cataaacatc
     1021 ataacgcccg cacgccagat aacaccagtc caacgtactg ctgcccatac tccgtatcgt
     1081 tccaaaaggc gcgagcgtac tcatgcggct ggacagtttg cccgaacgca aatatttgat
     1141 ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag gcaggcgggt
     1201 tccattaaga aacgcaccct gcccccgttc ggcataaaaa cattcgccgc tgaccgggtt
     1261 gtagattacg cccaattccg cacgcccgtt gcggacaaac gccaccgaca ccgcaaaatg
     1321 cggcagcccg ttgacaaaat tgttcgtccc gtcgatcgga tcgacaatcc acagcccctt
     1381 ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt cctcgcccaa
     1441 catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaat gccgtctgcg cggcaatgtc
     1501 cgcctcgctc aacatcgaac catcttcctt gcggcaagac ggcgtattca aaaaacgcgg
     1561 cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta acacttccta
     1621 ctgtcctcat attttgaact tgcggcgcgc gaacg
//
LOCUS       DAWKOD010000228         1643 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000228 DAWKOD010000000
VERSION     DAWKOD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.228"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      75..119
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            123..596
                     /gene="queF"
                     /locus_tag="WJK73_002007"
     CDS             123..596
                     /gene="queF"
                     /locus_tag="WJK73_002007"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ7558428.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            671..1357
                     /locus_tag="WJK73_002008"
     CDS             671..1357
                     /locus_tag="WJK73_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ7558429.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT          371 a          497 c          355 g          420 t
ORIGIN      
        1 acggaaaacg ccgccgtcca aaccatgccg tctgaagaaa actacacaga taccgccgct
       61 tatattacaa tcgccgcccc gtggttcgaa aacctcccac actaaaaaac taaggaaacc
      121 ctatgtcccg caataccgaa gagctgcaag gcatctcgct tttgggtaat cagaaaaccc
      181 gatatccgac cggctacgcg cctgaaattc tcgaagcatt cgacaacaaa catcccgaca
      241 acgactattt cgtcaaattc gtctgcccag agttcaccag cctctgcccc atgaccgggc
      301 agcccgactt cgccaccatc tacatccgct acattccgca catcaaaatg gtggaaagca
      361 aatccctgaa actctacctc ttcagcttcc gcaaccacgg cgattttcat gaagactgcg
      421 tcaacatcat catgaaagac ctcattgccc tgatggatcc gaaatacatc gaagtattcg
      481 gcgagttcac accgcgcggc ggcatcgcca ttcatccttt cgccaattac ggcaaagcag
      541 gcaccgagtt tgaagcattg gcgcgtaaac gcctgtttga gcacgacgca caataaacca
      601 tccttcccat atttcagacg gcattccttc gatacggtat gccgtctgaa cgcctttccg
      661 aaagattttt atgtacgcat ttactgccgc acagcaacag aaggcactct tctggctggt
      721 gcttttccat atcctcatca tcgccgccag caactatctg gtgcagttcc ccttccaaat
      781 tttcggcatc cacaccactt ggggtgcgtt ttccttcccc ttcatcttcc tcgccaccga
      841 cctgaccgtc cgcattttcg gttcgcactt ggcacggcgg attatctttt gggtgatgtt
      901 ccccgccctt ttgctttcct acgtcttttc cgttttgttc cacaacggca gttggacagg
      961 cttgggcgca ctatccgaat tcaacacctt tgtcggacgc atcgccttgg ccagctttgc
     1021 cgcctacgcg ctcggacaaa tccttgatat ttttgtgttc aacaaattac gtcgtctgaa
     1081 agcgtggtgg attgcaccga ccgcatcaac cgtcatcggc aacgccttgg atacgttggt
     1141 atttttcgcc gttgccttct atgcaagcag cgatgaattt atggcggcaa actggcaggg
     1201 catcgctttt gtcgattacc tgttcaaact caccgtctgc ggtctgtttt tcctgcccgc
     1261 ctacggcgtg attctgaatc tgctgacgaa aaaactgacg accctgcaaa ccaaacaggc
     1321 gcaagaccgc cccgcgccct cgctgcaaaa tccgtaaaac agccctgccg ggaatcggga
     1381 cggttgggtt ttcttttatt tgcaaacact gtcggaacgg ataaatccgg attcctacct
     1441 acttcgtcat tcccgcgaaa gtgggaatcc ggtgcgttgg gtttccatca tttccgataa
     1501 actcctgccg cgttgcattt ctagattccc gctttcgcgg gaatgacggc ggatgggttt
     1561 ccgtaccgtg gtgtccggat tcccgcccgt acgggaatga cggcagtgct tccttggctt
     1621 agttttattt taatcgactg tat
//
LOCUS       DAWKOD010000229         1617 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000229 DAWKOD010000000
VERSION     DAWKOD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.229"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            53..1222
                     /gene="metZ"
                     /locus_tag="WJK73_002009"
     CDS             53..1222
                     /gene="metZ"
                     /locus_tag="WJK73_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ7558430.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALESAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1308..1514
                     /locus_tag="WJK73_002010"
     CDS             1308..1514
                     /locus_tag="WJK73_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ7558431.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          391 a          425 c          436 g          365 t
ORIGIN      
        1 aaacaacggg cgcacgctcc gttgccgaac ggataaaacc gaggaatcac cgatgagcaa
       61 aaaactccat ccgcaaacgc tcgccatacg cggcggcaaa gaacaaaccg gataccgcga
      121 acacaatcag gcattgttcc tgaccagcag tttcatgtgg gacaacgccc aacacgctgc
      181 cgatttgttt tcaaaaaaaa tcaaaggctt cacttatacc cgtaccgcca acccgaccac
      241 cgccgccttt gaaaaacgca tcgccgcttt ggaaagtgcg gaacgcgcgg tcgccacttc
      301 gaccggcatg tccgcgattc aggcggcgtt ttttaccttt ttgcaggcgg gcgaccatgt
      361 gatttccagc cgcagcctgt tcggcacgac cgtcggcttt atcaataaca tcgttaccaa
      421 attcggcatc gaagtgagcc acgtgtcgcc gactgatata aacgaatgga aagccgccgt
      481 caaagccaac accaaactgc tgtttttgga aacgccgtcc aatcccttgg gagaagtggc
      541 cgacttggaa gccttggcgg aattggcgca cggcatcggc gcgattttgg tggtggacaa
      601 cagcctgctg tcgcccatcg gctctcagcc tttgaagcac ggcgcagata tttccgtttc
      661 ttccgcaacc aaagccatag acggacacgg acgcgtgatg ggcggcgtgt tggcgggttc
      721 ggaagaactg atggcgcagg ccgccgttta ctgcaactct tgcgggctgg cgatgtcgcc
      781 gtttaacgcg tggcagttgt tgagcggcgt ggaaaccctg tcgctgcgta tggaaaaaca
      841 gttcgacaac gccctgaaaa tcgcgcaatg gctgcaagcg cagccgcagg ttcaagccgt
      901 gtattacacc ggattgcccg accatccgca ggcggcgctt atccgcaaac agcaaaacgg
      961 cggtggcatc gtcatcggct ttgaagtagc cgaccaagaa gccgcgtgga aagttgtgga
     1021 cggcgtggaa ctcttttccc gaaccgccaa cctcggcgac gtgcgctcga ccatcaccca
     1081 tccgtggaca accacgcacg gcaggatgca gcccgaagaa aaactcgccg ccaacatccg
     1141 cccgggactg gtgcgcctgt cggtcggttt ggaatatgtt ggcgatttga ttgacgattt
     1201 gaaacaggca ctcgcacgct gattgcgtgt ttcagacggc attggacagc agatgccgtc
     1261 tgaaaccctt atcgcgtatg gcatacacat caacaatagg aaaaaatatg gatgaagcag
     1321 tttttgccga ttgggcgttg aaaatctgcc tgaccggctt gattattttt ttgggtttta
     1381 tcgtttggaa tttgggcaag gagtccaaag cgggcaaatt cggcatcgcc attttgtttt
     1441 tggttttggg cttgggcgtg ttcggttttg tgttcaaaga gttattgatt aagtttttgg
     1501 tattgccgaa ataggtttgc ggcctgtcaa aatgccgtct gaaatcataa aaagtaatct
     1561 gcaccctaaa agtttgattt aaccgccaac tgattaaggt gcggattttt atgacaa
//
LOCUS       DAWKOD010000230         1563 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000230 DAWKOD010000000
VERSION     DAWKOD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.230"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJK73_002011"
     CDS             complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJK73_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ7558432.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT          292 a          461 c          477 g          333 t
ORIGIN      
        1 aatgtaaaag gccgcctgaa aacctttcag acggcatcgt gccggattcc gcgtcagatt
       61 gcgctgccgc cgacggtcag cccggcatcg atacgcaggg tcggctgccc cacgccgacg
      121 gggacgctct gcccttcttt gccgcacacg ccgacaccgc tgtccaacgc ggtatcgttg
      181 cctatcatcg aaacgtgttt cagcacttcg ggcccgttgc cgataatggt cgcgcctttg
      241 acggggtatt gcagcctgcc gccttccacc caccatgctt cggacgcgct gaacacaaat
      301 ttgccgctgg taatgtccac ctgtccgccg ccaaagttga cggcgtaaat gcctttgtcg
      361 atggacgcga tgatttcttc cggctcatag ctgccatttt ccataaaggt attggtcatg
      421 cgcggcatag gggcggaagc gtaactttcg cggcggccgt tgccggtgga ttgcgtaccc
      481 gtcaggcggg cgttggtttc gtcctgcata tagccgacca aaataccgtc ttcaatcaat
      541 acggtgcggc gggtttcgtt gccttcgtcg tcgatattga gcgaaccgcg ccggccggca
      601 atatcgccct gatcgacgac ggtaacgcct ttggcggcga cgcgctcgcc gattctgccg
      661 gaaaagacgc tggtcccctt gcggttgaaa tcgccttcca aaccgtgtcc gaccgcttcg
      721 tgcagcaaca cgcccggcca gccgttgccc aaaacgacgg tcatttcgcc ggcgggcgcg
      781 gggcgggatt cgaggttggt gagtgcctgt ttgacggcgg catcgacaaa ctgccgcacc
      841 aagttttcat cgaaataagc caaatcataa cgtccgccgc cgcccgcgct gccctgttcg
      901 cggcgttcgc cctgtttggc gataacggta acgttcaggc gcaccatcgg gcggatgtcg
      961 gcggcgtgtt tgccgtccag acgggcgagg taaaccatgt cgtattcgca ggtcaaaccg
     1021 gccatcactt gcacgatgcg cggatcggcg gctttggcga ttgcttccac tttgttcaac
     1081 agcgcgactt tggcggcgga atcgaggccg gcaatggggt cggacgcgga acaaaccggc
     1141 ttgccgcgcg tttcagacgg cattttggcg gacaccttgc cgcctgccgc cccaatcgcg
     1201 cggacggcgc gggcggaacg gtttatcgaa tcgatgcaca ggctgtcggc gtaggcaaag
     1261 gcggttttgt cgcccgaaac ggcgcgcacg cccacgccct gattgatttg gaagctgccc
     1321 gatttgacga tgccctcttc caaatgccag ctttcataag cggtgcgctg gcagtagatg
     1381 tcggcgtaat cgacgtggtg cgcgccgatg atgcacaggc ttttggcgag cagttcgggg
     1441 gaaaggcggt tggcttcgag cagccgcgcc tgtacggcgg aataggtcgg atgcatagtg
     1501 tcggcgcata aaaaatcagg ggcttgatta tacggcattt gttatatagt ggattaacaa
     1561 aaa
//
LOCUS       DAWKOD010000231         1488 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000231 DAWKOD010000000
VERSION     DAWKOD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.231"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..533
                     /gene="mafB"
                     /locus_tag="WJK73_002012"
     CDS             <1..533
                     /gene="mafB"
                     /locus_tag="WJK73_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7558433.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
     gene            536..853
                     /locus_tag="WJK73_002013"
     CDS             536..853
                     /locus_tag="WJK73_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558434.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHADMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            987..1487
                     /locus_tag="WJK73_002014"
     CDS             987..1487
                     /locus_tag="WJK73_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-containing protein"
                     /protein_id="HEZ7558435.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAERKGFGFIMRVRQEKHHLPPYMGMIQKHMQQQR
                     NWDIEK"
BASE COUNT          498 a          303 c          323 g          364 t
ORIGIN      
        1 ttgaccagtg gatgcaggaa aaccccaatg ccgccgaaac cgtcgaagcc ctggtcaacg
       61 tcctgccgtt tgccaaagtc aaaaacctga caaaggcggc aaaaccgggg aaggctgcgg
      121 ttagtgggga tttctcagac tcctacaagc ataacactgc ttcaagatta tctcagtctg
      181 tagatggaga aatgtttcaa acccgcaatg ttgattttaa agcaaaatct attgggacta
      241 aaattcatga tggagctcaa gggaaacata tttcaggaca tagaaactac attgaaggta
      301 agagtacttt aaatcaaaac attaatcctc aagaattgtt gaacggaata cattcaggtg
      361 cttatccagt tatttctaaa ggagcaagag gaaatcctgt tgttgatttt gggtatccta
      421 taggcagcga tgggaaatca ggattaagta ccaattttgg tacgattcat tcaggtaaaa
      481 atggagttca cattgttccg gctaacccta aaaccattaa aaaggtgcaa tagttatgaa
      541 tatattacca agctggctgc gagtcggtat gaatatagca atgctgggca tgatacactc
      601 agatatcagg ttaattaccg tagattacga ggaaggaaga aggtttttaa aaatcaaaaa
      661 ttatttatca agagaagcca tcacagaaga ccatgcagat atggaatatt tgattacaga
      721 gttatggtct atgtgtggag aatattttga tgaagctgac tttgaatgta tttattctaa
      781 tcattcttct atggagttaa accaaataaa tggtgcagta ttcaggagaa aggaattaat
      841 ttcgcaagcg taggttaaaa aaccaacaat cacaatgtct tctgaaaccg tgtttaattt
      901 tcagacggca tttccttcat ttgaaatagg atattgagaa ctgagttctt caaaaatcct
      961 acacctgctc cttccacggc agcaccttgg tcaaaacggc agacggctac aaagccattg
     1021 cccgtatccg aaccggcgac cgcgtcttcg ccaaggacga ggcaagcgga aaaacgggat
     1081 acaaacccgt taccgcccga tacggcaatc cgtatcaaga aaccgtttac attgaaattt
     1141 cagacggcat cggtaaaata cagaccctcg tttccaataa aatccacccg ttttacagtc
     1201 aaggaaagtg gatacaggca ggtcgtctga aaaaaggcga caccctgctt tccgaaagcg
     1261 gtgcaaaaca gacggttcaa aacattaccc tcaaacagca gccgctcaaa gcctacaatc
     1321 tgaccgttgc cgactggcat acctacttcg tcaagggtaa tcaggcggaa cggaaggggt
     1381 ttgggttcat aatgcgtgtc cgccaagaaa aacaccatct acccccgtat atgggaatga
     1441 ttcagaagca tatgcagcag caaagaaatt gggatataga aaaataaa
//
LOCUS       DAWKOD010000232         1464 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000232 DAWKOD010000000
VERSION     DAWKOD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.232"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            47..1384
                     /locus_tag="WJK73_002015"
     CDS             47..1384
                     /locus_tag="WJK73_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7558436.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDEH
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKNAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          272 a          353 c          381 g          458 t
ORIGIN      
        1 tttttgttaa tccactatac tatcttcaca tttcttaata aattttatga gtaaccatac
       61 ttcttggtcg tccaaaatcg gtttcgtgct tgctgcggcg ggttcggcca tcggtttggg
      121 cgcgatttgg aaatttcctt atacggcagg caccaacggc ggcgcggtgt ttttcctgct
      181 gtttttgata tttaccgtct tggtcgccct gcccgtccag cttgccgagt tttatatcgg
      241 gcgcacaggc ggtaaaaatg ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg
      301 gctttgggtc gggcgcatgg gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt
      361 ggtcggcgga tgggtattaa attatgtcgt ccacagtttc acgggggcgg ttcataccgg
      421 cgcggacttt gaagccttgt tcggcacgac gatttccaat ccggcaggtt cgctgtccta
      481 tcaagcactg tttatgctga ttacggtttg ggtggtcaaa ggcgggattt cagacggcat
      541 tgaaaaggca aaccgttatc tgatgccggg actgtttatc ctctttattg cgctggcaat
      601 ccgttcgctg acgctgccgg gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg
      661 gtcgtacttt aaagccgata cgatgattac ggctttggga caggcgtttt ttgccctgag
      721 catcggcgtt tccgccatga ttacctacgc ttcgtatttg ggaaaagatc aggatatgtt
      781 ccgttccggc catacgatta tgtggatgaa cctcttggtt tcgctgcttg ccggcctggt
      841 gattttcccg gcggtgttcg ccttcggttt tgaaccgagc caggggccgg gattgatttt
      901 tatcgtattg cccgcagtgt ttatgaagat gccgttcggt acggttttgt ttgcggtatt
      961 tatgctgctg gtcgttttcg ccacgctgac ttcggcattt tcgatgttgg aaacggtcat
     1021 tgccgcaacc atccgccaag acgagcacaa acgcaaaaaa cacacttggc ttatcggcac
     1081 ggccattttc attgtcggca tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa
     1141 ggttttcggc aaaaccattt ttgatttgtg ggactatgtt atttccgccg tcattatgcc
     1201 gattggtgct ttgagtgttt ccatctttac cgcctggatt caggacaagc agtctgtgtt
     1261 aaaaaatgcc ggcgcgggca gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac
     1321 cttgcgctac cttgccccga ttgccattat tattgttttc atcaattctt tggacatcct
     1381 ttaaaagcca tccaaacagc aaaaatgccg tctgaaagcc tttcagacgg catttttgct
     1441 tcaggttcag cctatttcgt tcaa
//
LOCUS       DAWKOD010000233         1429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000233 DAWKOD010000000
VERSION     DAWKOD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.233"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..178
                     /locus_tag="WJK73_002016"
     CDS             <1..178
                     /locus_tag="WJK73_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558437.1"
                     /translation="ALIAAKIDQAEISDSEKSWLKKELGKIKDTALSTLTENAINAIL
                     AATLITLLKKFVGL"
     gene            complement(175..681)
                     /locus_tag="WJK73_002017"
     CDS             complement(175..681)
                     /locus_tag="WJK73_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7558438.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(684..881)
                     /locus_tag="WJK73_002018"
     CDS             complement(684..881)
                     /locus_tag="WJK73_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558439.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(878..1144)
                     /locus_tag="WJK73_002019"
     CDS             complement(878..1144)
                     /locus_tag="WJK73_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558440.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1156..>1429)
                     /locus_tag="WJK73_002020"
     CDS             complement(1156..>1429)
                     /locus_tag="WJK73_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558441.1"
                     /translation="SRAREQEPFVIHVSDLLDKAGIDYAVRMDKDFQTTFHLEYPITN
                     YDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVPV"
BASE COUNT          344 a          405 c          310 g          370 t
ORIGIN      
        1 ggctttaatt gcggcaaaaa tagaccaagc ggagatttct gattcagaaa aaagctggct
       61 gaaaaaagaa ttagggaaaa tcaaggacac tgcattaagt acgcttacgg aaaatgcaat
      121 taatgctatc cttgccgcca ccctcattac tcttttaaaa aaatttgtcg gcctctaacc
      181 aaacttcttc tccacttcca ccccaaacgg ctccaatacc tccacaaagg caggcaaatg
      241 tttggggtgt ttttcttgca caaacgccat caagaactca atcaattcca aagccgtcgc
      301 cagttttgac gtttccggca tcacacgcgc attggcggat acggttttcg taaacgcatc
      361 ggccaggctg gccaacagct tggcgcggtc ggacgggggc aaatcttcgg tactcgaatc
      421 ctgcagcatc gtcatcgtgc tgttgtactg caccataaaa ccggccaaca tcgcacggct
      481 caagtcctca ataccgccgc cggccaaagt gtaggcggcg cgcatcttat cccagtcgtc
      541 gcctttttcc ttatccgcac gtttccacgc acgcgcagtg gtctgcggga tttcgcacat
      601 caaggccgcc gtttccaaag tttgctcgcc gctcacatag agccgacgta acttttcacg
      661 gatttcttgc gggtgagcca taattacagt cccattttcg ctttaagcaa ttcccagccg
      721 accgtaatca cgccgccgcc cagtgcgccg aatgtaatgg ccgtgcgttt cgtgtcttgg
      781 cggatttgcg caatttccgc ctgcatttct ttctgatttt tcagcgtttg atcagtcttg
      841 ttttcaatac gcgccaaggc ttccaaaatc gggtcgctca tgatttgtct gctttcctgt
      901 ccagtttttc gtttactttt tccaacttgt tttcgatgcg tcccaacgaa gccgcaatat
      961 tgtcgcggtc tgccttggca tcttgcttgg tgtgataaga gagcttgacc gcgtgcagct
     1021 cctctttcag gtcgtcgata cgcttgtccg cctctttcag acggcctgaa atgccgttga
     1081 cccaaaacca aaatgccgcc gtcgcaatcg gccacagggt tctgaaacca aattcaaagt
     1141 ccatttaaaa cccctttaaa ccggcacatc gccgaatacg atacggacgg cgtaaccgtc
     1201 agggcgattg ctggcaatct ccagtccatc accatcatta caaacacagt aatacgcccc
     1261 caaagtttgc caaaccgcac gtttaaaggt gtcatagttc gtaattggat attcaaggtg
     1321 aaacgtcgtc tgaaaatcct tatccatgcg taccgcgtaa tcgataccgg ccttatccaa
     1381 caaatcggaa acatgaatga caaacggctc ttgctcgcgt gcgcggctt
//
LOCUS       DAWKOD010000234         1414 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000234 DAWKOD010000000
VERSION     DAWKOD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.234"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WJK73_002021"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WJK73_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ7558442.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIECSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          378 a          412 c          308 g          316 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcga catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaatgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgaccg
      481 aggcgcgcgg cgagcctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgctgccta cgaagtcgta tcccaactcg acaacattac cattctgacc gatacggccg
      601 ttaaagaagt caaaacatct gacaacgaag cacaagtcat tttagaaaac ggcaaaatcc
      661 tgaccgcccg cctgcttttg gcggcagaca gccgtttttc gcaaacccgc cgacaactcg
      721 gcatttcttc agatatgcac gactacagcc gaaccatgtt cgtctgccgc atgaaacata
      781 ctctttccaa ccaacatacc gcttacgaat gcttccatta cgggcgcacc atcgcactgc
      841 tgccgctgga agaacacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggtatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgatcggcga tgcggctgtc ggtatgcacc
     1081 ccgttaccgc acacggtttc aacttgggtc tgtccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgagcagcgc ggtcaggaca tcggcgcggc ttccttgttg gaaaaataca
     1201 gcagcaaaca tatgctgcat gcccagccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgcg ccggcaaaac tgctgcgcgg tttggtattg cgtgccggca
     1321 acaacttccc gccgctgaaa aaactgatta ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttg
//
LOCUS       DAWKOD010000235         1400 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000235 DAWKOD010000000
VERSION     DAWKOD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.235"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(41..1030)
                     /gene="porB"
                     /locus_tag="WJK73_002022"
     CDS             complement(41..1030)
                     /gene="porB"
                     /locus_tag="WJK73_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ7558443.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          318 a          321 c          370 g          391 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgct acgaatttgt tttcgccttt gccttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaagtatt gtcgcgtttt gcatcatcaa ccgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaatt gtcttcaacc agtttcgcgt cttgttgctg tacggctacg gaagcgtata
      361 gggcatcatt gtcgtaaccg ctgaccaaac ggtgaatctg gtatttctca atcttcacgt
      421 catccacatc ctgatgtctt ttataggcac cgccatattg cacgaagaag ccgccgtttt
      481 tgtagttgaa gccggcgtgg taagattcgc tgttatgtct gcctgcattg tcgttaagcg
      541 cgtattgtac gctgccgctg aggccggcaa attcgggaga atcgtagcgt acggaaatga
      601 ggcgtgcctc gggttcggca attttgttta cacccaaata gtcgcttttg ctatcccaag
      661 gattgatgtc gccggtgtct ttcaggacgc tgttcaaacg gccgacgcgc aatttaccga
      721 agccgccttt caagccgatg aaggattggc ggttgcccca accggagtca gtaccggcga
      781 tagatgcttt ttgctcaacc tgccaaatgg ctttcaggcc gttaccgagg tcttcttggc
      841 ctttgaagcc gattttcgaa cccaaatcaa cgatgccggt accggtttca acgctataaa
      901 cttgaccgtc ctggtaatct agggagcggg aagtttctac gccggctttg atggtgccgt
      961 acagggtaac gtcagccatt gctgcaacag gaagggctgc caaagtcagg gcaatcaggg
     1021 attttttcat tgctgtattc cttttttggt taagaaattt aagcaggccg ggctttccaa
     1081 gccgcttagc tttgcattta ccgccgaggg gctcggcgat tacatgtctg aaactataga
     1141 tgttcctgtc tgaaaaaaca aacttctgcc tgtttttgtg tggaattcga tgtgcatttt
     1201 gaaggacgga tagagatatt atgggtattt tttgttttat aacatatggt tatttaaatt
     1261 ttttaagatt tgcattttta caacacttac tcgggagggt attggagggc attgcaaacc
     1321 gggggttata aagacggcaa aaaacgcaca cgcggtttct ttcggaacgg gcggatgcaa
     1381 aacccgcccc tgcggcagga
//
LOCUS       DAWKOD010000236         1364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000236 DAWKOD010000000
VERSION     DAWKOD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.236"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..1010)
                     /locus_tag="WJK73_002023"
     CDS             complement(87..1010)
                     /locus_tag="WJK73_002023"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ7558444.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVVIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          368 a          317 c          335 g          344 t
ORIGIN      
        1 attttaggtt tctgttttgg ttttttgtcc ttgtaggaat gatgaaattt taagttttag
       61 gaatttaccg gaaaaaacag aaagcgttat ccacaatttc tgatgttcag ctcgtgaaat
      121 gcgtcgggca aatcatcgct gtcggcaaat tccacccggt cgtaagccgt ttcgtctgcc
      181 aaaaccgcgc gcaagagtgc gttgttgatg gcgtgtcccg atttgtagcc ttcaaacgcg
      241 ccgacaatcg gatgtccgac gatatacaaa tcgccgatgg catcaaggat tttgtggcgc
      301 acaaactcat cgggatagcg caagccttca ggattcagga catccgtgtc gtcaatcacg
      361 atggcgttgt tcaaattgcc gcccaaaccc agattgtggg cgcgcatcat ttccacttcg
      421 tgcataaagc cgaaggtgcg cgcgcgcgcg atttcgtcga tgtaggattt gccggcgaaa
      481 tcaatttcaa aagtgggcga gctgcggttg aaaaccggat ggtcgaattc gatggtcagc
      541 gttaccttaa agccgtcata cggcgtaaag cgtacccatt tgcccgcttc tttgattacg
      601 acaggcttga ggattttcaa aaaacgcttt tgcgcctttt gatcgaccac gcccgcatct
      661 tgcaaaaggt aaataaacgg caggctggag ccgtccataa tcgggatttc gggcgcgttc
      721 agctcaatca gcgcattgtc gatgccgtag gcggacagcg cggacataat gtgttcgatt
      781 gtgccgacgc gcacgccttt gtcggtaacg atggtggagg aaaggcgggt atcgttgatc
      841 aaataagggg tcagtttgat ttgttcgccc atctcgccgt ccaaatcggt acggcggaag
      901 gaaatcccgc tgttttcagg cgcggggtgc agggtcagcg cgacgcgttc gcccgaatgc
      961 agcccgactc cggtaacgct gatggatttc gccaaagttc tttgcagcat aaaccgcttc
     1021 cttatcaatg gggtaagttt tggaataata cgataaaacc ggaaaaacaa gctatgtttt
     1081 tccatagtat ttgccaatgt atctgttttc aatacgtaag ccgcataaaa atgtatagtg
     1141 gattaacaaa aatcaagaca aggcgacgaa gccgcccctc tcgaaaaacg caaaaaatgc
     1201 cgtccgaaaa cctttcggac agcattttcg cgtgaaccgt cattcccaca aggacaaaaa
     1261 accaaaacat aaaaccaaaa acagaaacct aaaattcatc attcccacga aagtgggaat
     1321 ctagttcgtt gagtttcagt tatttagaat aaattttgaa actt
//
LOCUS       DAWKOD010000237         1309 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000237 DAWKOD010000000
VERSION     DAWKOD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.237"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            38..461
                     /locus_tag="WJK73_002024"
                     /pseudo
     CDS             38..461
                     /locus_tag="WJK73_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WJK73_002025"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WJK73_002025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ7558445.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WJK73_002026"
     tRNA            1210..1285
                     /locus_tag="WJK73_002026"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
BASE COUNT          315 a          312 c          320 g          362 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccctatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcat
//
LOCUS       DAWKOD010000238         1298 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000238 DAWKOD010000000
VERSION     DAWKOD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.238"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..398)
                     /locus_tag="WJK73_002027"
     CDS             complement(126..398)
                     /locus_tag="WJK73_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558446.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(960..>1298)
                     /locus_tag="WJK73_002028"
     CDS             complement(960..>1298)
                     /locus_tag="WJK73_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558447.1"
                     /translation="LSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFAS
                     TRSTLAATAGGMIGGGYSSAMLRGAGITTSVFARSAWKGGNAIPDAVIRANGAALNQS
                     SSAAMGKHRR"
BASE COUNT          411 a          308 c          186 g          393 t
ORIGIN      
        1 ccctgcttag aacgtatgtt gcacacccaa tttcaatagg taaatcagat tgcaaatcca
       61 gcaatttgaa tattgtcatt gttccgtgca aaagggattt ttattgatga gttgtgtact
      121 gggtttcagc ttggcttttt agataatctt ttcacaagaa catacatcaa tactgtccaa
      181 aaaaaaagat ttgataaata tattgcaaca tattttattc cacctccatc cgaaacagaa
      241 accaatatat ttttaattaa tacatagctg attattacat ttattattcc tataatatat
      301 aaggatcttg ccaaaatttt ttttgatttt attttaggaa tatctcttat ccatgctaaa
      361 atacagccca atgtcgaaaa gaaaataaca gcaaacacta ccttacctcc tctcacttcc
      421 aaccaaaccg atagcaggtt tggttggaag ttggtttatt tattatttaa cggcgaatgt
      481 cagtgttctt acccgtagaa cctgcataac catttatgcc accagcaaca gtccccactg
      541 ctacacctcg cataccatta atcagaattc ttcctatttt ttcgaatcca ctatttcttc
      601 caaagatgca gcaaacgcag gttgagccta gacccaatct acgtttgctg ccgcatctga
      661 tccctattgt ttcttatcct tacatcttcc tgccttgtca atcaaataaa gacagaagac
      721 tatacaaaaa ctgaccgaca tactgaaaat acctatcccc ttccatgcaa tctccgcacc
      781 attgacccaa tagattagac tgagaaacaa caaagccaca gtataaatca acgtaaaaaa
      841 tattgcgtaa agcacaataa agggaacttt tatctcacgg ttgtttttta taatatattc
      901 agcaacttga tttccgaata tacctgataa aaaatatagt attagatcat ccatttcgtt
      961 tatcttctat gttttcccat tgcggcgcta gaagactgat ttaatgctgc accatttgca
     1021 cgaataactg catcaggaat agcatttccc cctttccaag cagaacgggc aaaaacacta
     1081 gtagtaatcc ctgcaccgcg caacatcgca ctgctatatc cgccgcctat cataccacca
     1141 gcggttgcag ccaaggtact tcttgttgaa gcaaattgcc ctgttttaat tttagaaatg
     1201 ccgtgattag cccaagcact aattgcccct cccatcaaag cacctgccac aataggaaga
     1261 aacgccccct cagtctcctt catctccttt tgagaaag
//
LOCUS       DAWKOD010000239         1295 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000239 DAWKOD010000000
VERSION     DAWKOD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.239"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            174..1232
                     /gene="alr"
                     /locus_tag="WJK73_002029"
     CDS             174..1232
                     /gene="alr"
                     /locus_tag="WJK73_002029"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ7558448.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASVYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          328 a          301 c          348 g          318 t
ORIGIN      
        1 ttgggaaagc gattatatca ggaaaagcaa accgccttcc tacctaaaaa ctgctgcttc
       61 ggcttgaaga tacaagggtc tttaatattt taaaagcctt gccgttggat tataattccc
      121 taattgattt cttaattttg ctaataaaca cttgtttggt aaggaatgaa tttatgcgcc
      181 ctttgaacgt gcagatcagg ttgggcaacc ttaggcacaa ttatcggatt ttgaaggaaa
      241 tgcacggagg caaactgttg gcggtagtga aggccgacgc atacggacac ggtgcggtca
      301 gatgtgcttt cgcgctggca gacttggcag acggctttgc cgtggcgaca atcgacgagg
      361 gaatcaggct acgggagagt ggcattaccc atccgattgt ccttttggaa ggcgtatttg
      421 aagcatcaga gtacgaagcg gtcgaacaat actcgctctg gccggcagtc ggaaaccaat
      481 ggcaacttga ggctttgctt tcccgccatt ggaaaaaacc tgtcaaggtc tggttgaaaa
      541 tggattcggg aatgcaccgt accggatttt tccctcatga ttacgcttcg gtatatgcgg
      601 cattgaagca gtcggaatat gtggacagta ttgtcaaatt ctcgcatttc tcctgtgcgg
      661 acgaacccga aagcggtatg acggaaatac agatggaagc attcgatttg ggtacggaag
      721 ggctggaagg cgaagaaagc cttgccaact ccgccgctat tttgaatgtt cccgaagcac
      781 gcagggactg ggggcgagcc ggcttggcgt tgtacggcat ttccccgttc ggaggcagcg
      841 atgacaggct gaaacccgtg atgaggcttt ccacccgtat tttcggcgaa cgcgttttac
      901 agccgcactc ccctatcggt tatggcgcaa cattttatac cagtaaatct acgcgcgtcg
      961 gcctgattgc ctgcggttat gcggacggtt atccgcgccg cgccccaagc aattcccccg
     1021 tcgccgtcga cggtaaattg acccgggtca tcggcagggt ctctatggat atgatgacca
     1081 tcgagctgga cgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg ggcgatacgg
     1141 ttaatatcaa taccgttgcc gaagcggccg gaaccatccc ttacgaattg atgtgcaata
     1201 tcaaacgtgc aaaattcact tatatcgagt aatcaagtcc aaacaaaaat gccgtctgaa
     1261 gcctttcaga cggcataaat gcgttttgac aaaat
//
LOCUS       DAWKOD010000240         1255 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000240 DAWKOD010000000
VERSION     DAWKOD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.240"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..>1255
                     /locus_tag="WJK73_002030"
     CDS             15..>1255
                     /locus_tag="WJK73_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558449.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELG"
BASE COUNT          381 a          227 c          306 g          341 t
ORIGIN      
        1 taaaggatct tattatgaat gagggtgaag ttgttttaac accagaacaa atccaaacct
       61 tgcgtggtta tgctttccgt ggcgatacct atggcggttg gcgttatttg gctaatttgg
      121 gtgaccgtta tgcggatgat gctgctgcaa ttgtcggtaa ggatgcaaac ttaaatggtt
      181 tgaatttatg gatgaaaaaa ggtgtggaaa acctatggga tgatacggtc ggtaaaaaga
      241 cccgtttaga gaaatttgat cgggttgcat tgcaacattt cagccaatat gtagatctaa
      301 ttaatgaaaa taatggtaga ttacctaaca ctagtgaaat tgagagaagt tactataaag
      361 ccgttaccga aaatggtgtt tcttctagtg cagctattga tttagttatt aatcgctcac
      421 ttccggatat ggcagatggt tattgggcat taggtttggg gatagaagcc gaacgtatcc
      481 acaatgcgca agcagtaaat aatccgaacg gtagcgaatg ggataataga aagcagttaa
      541 tatctgcttt agataaagga tttgatggat cttttaaaga gaagcatttt acttttttac
      601 aatctgtgat aatggatgta acaaagttag gtgttgaata tacaatagat ggttggcaaa
      661 aaattggagg ttggggtaat gggataatca atgatttata taaaagtgtt gcaaaaagag
      721 agtggactgg aatatttgag atcgttaata ataacatcaa gcaatttaga gatctgttcc
      781 caaatccgga aggctggatc gatgatggtc accaatgttt cgctccttgg gttaaagaaa
      841 ctaaaaaacg caatggcaaa tatcatgtct acgaccccct cgccctagat ttggacggag
      901 acggtataga aaccgttgcc accaaaggct ttgcaggcag cttatttgat cacaccaaca
      961 acggtatccg caccgccacc ggttgggttt ctgccgatga cggtttactc gtccgcgatt
     1021 tgaacggcaa cggcatcatc gacaacggtg cggaactctt cggcgacaac accaaactgg
     1081 cagacggttc ttttgccaaa cacggctacg cggctttggc cgaattggat tcaaacggcg
     1141 acaacatcat caacgcggca gacgccgcat tccaaaccct gcgtgtatgg caggatctca
     1201 atcaggacgg catttcccaa gctaatgaat tgcgtaccct tgaagaattg ggtat
//
LOCUS       DAWKOD010000241         1232 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000241 DAWKOD010000000
VERSION     DAWKOD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.241"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            40..372
                     /locus_tag="WJK73_002031"
     CDS             40..372
                     /locus_tag="WJK73_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ7558450.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            704..1213
                     /locus_tag="WJK73_002032"
     CDS             704..1213
                     /locus_tag="WJK73_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ7558451.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGATQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          328 a          230 c          295 g          379 t
ORIGIN      
        1 ccttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcaa
       61 ccggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
      121 caattcgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt agcggcactg
      181 cttgcgcccg atatgccgcc gggcattccc tgtttttggg tggaaggcgc ggaaagcctt
      241 gaagagttgt gcgccatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
      301 gatgacgacg aatggctcga aacggcacag ggttgcgggc accacggcga cgcttgcgcc
      361 tgcggacatt aaaggcattg caggcttgcc gcaaggggtg caaggctttg ccgtttttta
      421 aataatcggt ggattgcatc tgtttgcaat gggcggaacg cttccctttg tattttattg
      481 aataaaaaaa cttatcttgg tattataatt aaggtagcat caattatttt gggatggcaa
      541 taaacgcaaa gcattgattt gcgccgattg cagacttatt atagcaggtt gcggcgcgga
      601 cttaatggga attgtttata gagcatgatg gtctgaaaca tcattaatta aaaataccat
      661 taatccgatt tatatttatt tcaatttcaa tggaaaaaca tcaatgacaa tgattttaag
      721 cattttaagc ctgtttttta tcatcagact gttattttta gccgtctcta ttaaacatga
      781 aaaagccttg attgccaaag gggcaacgca atacggaaaa accaattcca cagtgcttgc
      841 ggcagttcat acgctttatt atttggcgtg ttttgtttgg gtatggcttt ctgacactgc
      901 ttttaatggc atatccttga ttggtacgct gacggtgatg gcttcgtttg tgatattgtc
      961 attgattatt aagcagttgg gggagatttg gacggttaaa atctatattt taccaaatca
     1021 tcaaattaat cgttcgtggt tgtttaaaac attccgccac cccaattatt ttttaaacat
     1081 catacccgaa ctgattggca tcgccttatt atgtcaagcg tggtatgttt tattgattgg
     1141 cctgccgatt tatttgctgg tcttatttaa gcgtatccga caagaagaac aggcgatggc
     1201 aacacttttt taacccgttt catcaattat ag
//
LOCUS       DAWKOD010000242         1185 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000242 DAWKOD010000000
VERSION     DAWKOD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.242"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..456)
                     /locus_tag="WJK73_002033"
     CDS             complement(<1..456)
                     /locus_tag="WJK73_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558452.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          427 a          216 c          121 g          421 t
ORIGIN      
        1 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaattcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa gtagaaaatc
      901 aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta taaaattaaa
      961 taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtgag aatgcattat
     1021 tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc aatgcttgca
     1081 tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg ttcttttctt
     1141 gcaagatgtg cacctgcaca accacctaat actaaaccac cacct
//
LOCUS       DAWKOD010000243         1126 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000243 DAWKOD010000000
VERSION     DAWKOD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.243"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(229..1083)
                     /locus_tag="WJK73_002034"
     CDS             complement(229..1083)
                     /locus_tag="WJK73_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7558453.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKVRAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCSW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSVLGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          313 a          336 c          294 g          183 t
ORIGIN      
        1 ccgtcattcc cgcgcaggcg ggaatccgga catccaacgc tgcagcaatt tgtcggaaat
       61 aaccgaaact caaaaaaccg gatgcccgcc tgcgcgggaa tgacgatgca aagatggtcg
      121 ttatttcgga taaatatccg tcttttcgtt ccggataccc gctttcgcag gaatggagat
      181 ggaaagattt cagcctattc ggcaatcatc aaataccgct tcccaacttt atttttgatg
      241 cgacagccgg ccggcaacca aggtggcggc gatgacgaca aacacgccca agacggacac
      301 gggcgacagg tgttcgccca aaatcaaaac cgccagcagc acgccgacga cgggttcgag
      361 cgaaatcaac agtcccgaaa cgttggcagg aacacggctc atccccttgt tccacagcca
      421 ataggcgtac cagctgcacc ccacgcccaa atacagcagc gacaatacca ttccgacgct
      481 ccagtccacg gtataacttt gcgccaaagc aagcgaaaac ggcaggcaca tcaacgatgc
      541 ggcggcaatg gaaacagatg tgaatgccgg tgcgccgatg cgtgcaatca gcctttgcgt
      601 cggacgcata gcggcacaaa agcccgcgcc cgccaacaac accagcaggc agccgaacca
      661 gccgacttcg ccgccctctt ccgcaccgcc cgccatcagc agcgcgacac cggcaaatgc
      721 cgccgcgccg catatccagt ggtaggcacg cactttgtcg ttgaagaaaa agtgtccgac
      781 aaacaccatc agcagtggct cgagtccgac aatgaccgat gcgctggcgg cggaagtgta
      841 tttcaacccg acaaactgaa gcagcagggt cagcacatag ttgacgaacg acacaatcag
      901 caacggcttc cattcctcac gcggaatctt gccgacatga cggcggcagg cgggcagtgc
      961 aggcagcgca gcaatcagca ggcgcacgcc gaccatcaat gcgggatcga tgccgccata
     1021 gacatatttg gcggcaataa acgagctgct ccagataatc agggcaagga tttggtaaaa
     1081 catagtgagt gataaagtca gatggtcata caaaagtata gcggat
//
LOCUS       DAWKOD010000244         1126 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000244 DAWKOD010000000
VERSION     DAWKOD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.244"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            81..1046
                     /locus_tag="WJK73_002035"
     CDS             81..1046
                     /locus_tag="WJK73_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7558454.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQSESASL
                     TVKTAHGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          288 a          316 c          296 g          226 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttacgtt tgactgcttt agccgtatgc accgccctcg
      121 ctttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaatccgaa agtgcgtccc tgaccgtcaa aacggcgcac ggcgatgttc
      241 aaataccgca aaaccccgaa cgtatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgaccaaa cccgccggaa ctttgttcga gccggattac gaaaccttaa
      421 atgcctacaa gccgcagctc atcatcatcg gcagccgcgc cgccaaggcg tttgacaaat
      481 taaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagagcg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaggcgga aatcgatgct tcgtttgaag cggcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggtt tgcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtcctcga ccgaagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acaaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tctggcggcc ggcggcgcgc aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcgtt caacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcccgtg tttcagacgg cattgcccga tacggg
//
LOCUS       DAWKOD010000245         1112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000245 DAWKOD010000000
VERSION     DAWKOD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.245"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            153..1052
                     /locus_tag="WJK73_002036"
     CDS             153..1052
                     /locus_tag="WJK73_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7558455.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          187 a          256 c          355 g          314 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWKOD010000246         1063 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000246 DAWKOD010000000
VERSION     DAWKOD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.246"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..533
                     /locus_tag="WJK73_002037"
     CDS             99..533
                     /locus_tag="WJK73_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ7558456.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            514..942
                     /locus_tag="WJK73_002038"
     CDS             514..942
                     /locus_tag="WJK73_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ7558457.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
BASE COUNT          282 a          192 c          317 g          272 t
ORIGIN      
        1 ctgtccgttt cgcaaaaaaa acatcggctt aatactatat attgtatttt attgtataaa
       61 atgcgctaat ttatactata tgttgtattg gagagataat gcgccgggcg ttgattgcga
      121 aaattaagat tgctcaaaag gagctgggct tggatgacgg tacctatcgc gcggtgttgg
      181 agcgtgtgac ggataagcgg tcgtgtgcgg atatggatgt ttctgaactt gagtctgttg
      241 tcgctgatat gcggtcgcac ggatttaagc ctaaagcaaa aggtaaccca cacggcaaac
      301 cgcatctgcg tcggacatca tcagcggcaa tgctggacaa agtcgaagcc ctgctgaccg
      361 tcggcggcaa acattggaac tatgcacacg caatggcgcg gcggatgttt ggtaaggata
      421 aggtcgaata tttagacgat acgcagctac ataaactggt tgctgcgttg cagattgcgg
      481 aaaacaggaa aacggaaaaa gcgggtgggg atgatggggt tcgaaaaagt tgaacattta
      541 ttgccggata ccgtgttgga cattgtggat gtcatcggac tggcagcgac ggaacagctg
      601 gtcaaggcga ttggcggggc gcggtttaaa tttggtaagg gcaaggtgga caccgagcgt
      661 ttggcaattt tggtcgaagc catcggcgaa gtgaaaacac atgagctgtt gcaggtatat
      721 ggtggcgagg aattgtatgt cccacggtgc ggcaaggcgt taatacagtt gagaaaccat
      781 aggttttatc aggagtttgt caaattgcgc gatattgata agaagagcgg gcttatggcg
      841 atgacgaagc tatgccctaa atacggcatc tcttcacgaa cgggatatac gattatcaat
      901 gaaatgagcc gacctgcggc acagcaggca gctttatttt aggcagtgat gtgtgaccag
      961 gctttggccg tctgtattca gacggtcttt tttttggttt gcaggggtga aacatctacc
     1021 gttcgggacg atgggttaaa gacggtttaa tggggttttc aaa
//
LOCUS       DAWKOD010000247         1028 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000247 DAWKOD010000000
VERSION     DAWKOD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.247"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(247..972)
                     /locus_tag="WJK73_002039"
     CDS             complement(247..972)
                     /locus_tag="WJK73_002039"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ7558458.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFA"
BASE COUNT          175 a          226 c          349 g          278 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gagggtttgt tgtttcggat aaatccctgt agtttttcgt
       61 ttctagattc ccgcctgcgc gggaatgacg gcggagaggg ttgttgtttc ggataaatcc
      121 ctgtagtttt tcgtttctag attcccgctt tcgcgggaat gacgacacgg gaaatcttat
      181 ttgaacctac tatgtcttaa tcggctgcaa aatgccgtct gaacgggttt cagacggtat
      241 ttctgtttat gcgaaatcgc cccaaagtgt ctgcatcgcg gcgatggcgg cgagtggggc
      301 ggtttctgtg cgtaaaatcc gtttgccgag tgtaaccgcc tgaaagccgg cttcaaatgc
      361 ctgttgttct tcctgttctg tccagccgcc ttcgggcccg accataaaga cgattgcgcc
      421 ggacgggtgg cggatgtcgc cgagtttgcg ggcgcggttg atgctcataa tcagcttggt
      481 gctttcagac ggcattttgt cgagtgcttc acgatagccg atgatgggca gtacgggggg
      541 aacggtgttt ctgccgcttt gttcgcacgc ggagatgacg atttcctgcc agcgtgcgag
      601 gcgtttggcg gcgcgttccc cgtcgaggcg gacgatgcag cgttcgctga tgacgggctg
      661 tatggcggtt acgccgagtt cgacgctttt ttgcagggtg aaatccatgc gatcgccgga
      721 ggagatggat tgtatcagtg taatgttgag cggggattcg ttgtcggttg tgtcttcgtg
      781 caggatttcg gcttcggcgc ggcgtttttc caaaaaagac agccgtgcgg cgtgtgcctt
      841 gcctttgccg tcgaagaggg tgatgttttc gttggggcgg acgcgcagga cgttgaggtg
      901 gcggacgatg ttgtcgggca aggcgacggt ttgtccgacg gaaaggtttt cgggcaggtg
      961 gaatcggggc atagtttttg gcggttcgga cggttataat ctgtcgcagc ggcgtgatgg
     1021 aaacggac
//
LOCUS       DAWKOD010000248         1020 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000248 DAWKOD010000000
VERSION     DAWKOD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.248"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..>1020
                     /gene="tuf"
                     /locus_tag="WJK73_002040"
     CDS             32..>1020
                     /gene="tuf"
                     /locus_tag="WJK73_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7558459.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEG"
BASE COUNT          276 a          285 c          252 g          207 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
//
LOCUS       DAWKOD010000249          950 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000249 DAWKOD010000000
VERSION     DAWKOD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.249"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            220..891
                     /locus_tag="WJK73_002041"
     CDS             220..891
                     /locus_tag="WJK73_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7558460.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          273 a          214 c          211 g          252 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttgg aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcatagactg agacctttgc
      901 aaaattcccc aaaatcccct aaattcccac caagacattt aggggatttc
//
LOCUS       DAWKOD010000250          949 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000250 DAWKOD010000000
VERSION     DAWKOD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.250"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(30..221)
                     /locus_tag="WJK73_002042"
     CDS             complement(30..221)
                     /locus_tag="WJK73_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558461.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            350..910
                     /locus_tag="WJK73_002043"
     CDS             350..910
                     /locus_tag="WJK73_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ7558462.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          266 a          267 c          191 g          225 t
ORIGIN      
        1 aaggcgagac aacgacgtac cggtttttgt taatccgcta taccacgatg aatccttcgc
       61 aatatctgtt tatcgacctc aattttgaca aaataccgga tacgcgcctt tgttgctttt
      121 ccatcttcca accaactgta aatctcaaac agccggtaca cgccatgctt cagtttcttt
      181 tcctgtcggc ggattgtttc gacaaagaat tgaaaatcca tttcatgcac cttaaaattt
      241 aatctgcatt caaacctttt cactttggaa gcaccattta tcggatgtcc cttcgcaata
      301 aacaaatttt cccgataccg ccgcccattt caacccaaac ccaaaaccta tgaaaaacct
      361 catcgccttc aacaaaccct atggcgttat ctgccaattt tcaccgcacg aaaaacacaa
      421 aagcctcaaa gactttatca attttcccgg cttctacccc gccggacggc tcgacaccga
      481 cagcgagggg ctgctgctgc tgaccgacga cggcaggctt caggcacaaa ttaccgaccc
      541 caaattcaaa caccctaaaa cctactgggc gcaactggag ggcgtacccg acgaaagccg
      601 attggaaagc ctaagaaaag ggatagactt aggcggtttc gttacccgtc cggcaagcat
      661 ccgcatcttg aaacacggag aagcagattc gttatgggag cgcatcccgc cgatacgcgt
      721 ccgcaaaacc gttcccgatt tttggattga aattaccatt tctgagggca aaaaccgcca
      781 agtcaggcga atgaccgcca aggcgggcta tccctgcctg cgtctgatca gagtggcaag
      841 cggcaggctg aaactgtttg atttggattt aaaacccggg gaatgggcat acgccccgtt
      901 taaaccataa tcacgtttat ctcatcattt ccacaaaagt gggaatccg
//
LOCUS       DAWKOD010000251          902 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000251 DAWKOD010000000
VERSION     DAWKOD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.251"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJK73_002044"
     CDS             complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJK73_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ7558463.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          200 a          201 c          237 g          264 t
ORIGIN      
        1 tatttttaga agaaggcttt gtgtcatttt gatgccgtct gaaagggttt gttcgtttca
       61 gacggcattg ttaccaaggc ttgattattt ccggcgcagg tcgggatggt tttccaagtt
      121 gtccatcatt atcccgatga tgcgcggggc ggttttgccc aaatcgaaac gttcgcagga
      181 agagaaccac cgccaaatca gcccgtccaa ggttgatttg atgaagatga ccgctgtttc
      241 cttgtccaaa tcgtcagcca aatcctgatt ttccaccgct tcggtcaaaa cggcggtaat
      301 tttctcgcgc cagattgcct gatgcttgcg ggcaatggcg ataacggcgg cgttttgctc
      361 cgtgtgttcg cattttaaaa acaggatgtt gtggaatttg tagtagatgt cgttgctttg
      421 cagccgctcg aaaaagtgca gcagcgtgtg gcggaatacc gcccaagacc ctccttcggc
      481 atcttcggca tcttgcgcga tgcagttttc gatgtcgtcg cagatacgtt ggaacagcgc
      541 gtcgaacaag tcttccttat ttttgaaatg ccaatagagc gcgccgcgcg ttacgccggc
      601 ggcttgggcg atttcgttga gcgaggtgcg cgcaatccct ttgcggtaaa aggtttccaa
      661 ggcggcaagc atcaggtgtt ctttggtttt caaggcttcg gttttggttt ttctcataat
      721 ggcgttttcg tttcgggtcg gtttgatgag ggcggattat aaaaaagact ttgtaaccat
      781 gcaatcgtgt atgtataatg aaacccatga aattgagact acatctcaac tttgaaaaca
      841 ggcataacct gctttgcaac ccgttcgaac attcgggtat caaagcaggc ggtttttata
      901 tc
//
LOCUS       DAWKOD010000252          874 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000252 DAWKOD010000000
VERSION     DAWKOD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.252"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(55..834)
                     /locus_tag="WJK73_002045"
     CDS             complement(55..834)
                     /locus_tag="WJK73_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ7558464.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          203 a          288 c          174 g          209 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttcca cgatgcggcc
      781 ttccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg ccgaaccctt ccga
//
LOCUS       DAWKOD010000253          829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000253 DAWKOD010000000
VERSION     DAWKOD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.253"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          198 a          204 c          220 g          207 t
ORIGIN      
        1 agggctaatc tagtacagcc ccttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaggatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gcgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagacaacct ttgaatattg ccgccgtccc
      541 gcgttctaga ttcccgcctg cgcgggaatg acgattcatc agtttcccga aacgcccgaa
      601 aaaccgaaac cgagcaaacc ggattcccgc ctgcgcggga atgacgattt ataagtttcc
      661 cgaaacgccc aaaaaaaccg aaatccgaca ggcgggattt ttgctttcgt ggggatgacg
      721 ggattttaag ttacggtcat ttatcggaaa agtagaaatc gctccgccgt cattcccgcg
      781 aaagcgggaa tctgaacacg tccgcacgga aacctatagt ggattaaca
//
LOCUS       DAWKOD010000254          728 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000254 DAWKOD010000000
VERSION     DAWKOD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.254"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            141..575
                     /locus_tag="WJK73_002046"
     CDS             141..575
                     /locus_tag="WJK73_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7558465.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(624..>728)
                     /locus_tag="WJK73_002047"
     CDS             complement(624..>728)
                     /locus_tag="WJK73_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7558466.1"
                     /translation="LRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
BASE COUNT          218 a          205 c          170 g          135 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cgttatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct tccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaactcaa atgcgaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgatgtgaac aaatactgcc gttgagcagc ctcatcgaaa accccatgcc
      601 gtctgaaacg gcacggggtt tgtttatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaa
//
LOCUS       DAWKOD010000255          726 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000255 DAWKOD010000000
VERSION     DAWKOD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.255"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(129..>726)
                     /locus_tag="WJK73_002048"
     CDS             complement(129..>726)
                     /locus_tag="WJK73_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7558467.1"
                     /translation="LSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          162 a          210 c          197 g          157 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc catcccttcc gaataatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaac ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc ggattaaccg gtttgccgtt ttgccgcacc
      181 tcgaaatgaa gctgcgttct ggaagcgtcg gtattgccca tcaaagcgac ctgctgcccg
      241 cgtttgacct gctggccttc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaggaagaat tatgctggat gatgaccaga ttgccgtatc ccctcaaacc ggaacctgca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgcgg ttttcggtgc ggcatatcct gccggtttga ctttaacaat ctgaccgatg
      721 ctcaac
//
LOCUS       DAWKOD010000256          708 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000256 DAWKOD010000000
VERSION     DAWKOD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.256"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..641)
                     /locus_tag="WJK73_002049"
     CDS             complement(90..641)
                     /locus_tag="WJK73_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558468.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          199 a          275 c          135 g           99 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgcag gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga taaactcg
//
LOCUS       DAWKOD010000257          691 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000257 DAWKOD010000000
VERSION     DAWKOD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.257"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            231..530
                     /locus_tag="WJK73_002050"
     CDS             231..530
                     /locus_tag="WJK73_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7558469.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPASMAVSSLPPQPH"
     gene            509..691
                     /locus_tag="WJK73_002051"
                     /pseudo
     CDS             509..691
                     /locus_tag="WJK73_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          183 a          197 c          144 g          167 t
ORIGIN      
        1 caaacatcaa attggactga ccaatcaggg cggattctaa tgacacgccg ttcccgccgt
       61 caacggcatt tacctcgcac cgcccccgaa acacaagaaa aaactacacc aaactacaat
      121 ttttgttcat gcaaatattt gttttgacag gatttaaacg aaaactccga ttcaaatctg
      181 ccgaaccgcc caaaaatata ttgacctaaa tattaaagtt tcgtaaagta atgcaacgtt
      241 gctttaattg gtttgaccac tattgccgac gattagaaaa atattttcgg agatgttcaa
      301 ttatggaaac ttgggttcaa aactacacgg caatcggcgg cagcctgtat ctgactgccg
      361 ccgccgcact cttacccatc gtctttttct ttgccgcgct gaccgtcctg aagctgaaag
      421 gctatcaggc ggggctttat acgctgctga ttgcgcttgc cgttgccgta ttcggcttcg
      481 ggatgccggc gagtatggcg gtttcttccc tgccgccgca gccgcactga cccaacgccc
      541 ctacgccacg ctggatttga ccgcgcatta caaaatcgga aaatccacac gcatcggttt
      601 ggactttgaa aacgtgttca acaaacgcta ccgcaccatg cccgacattc acgtttacgg
      661 cacgccgcgc agcctgaccg caaccgtcaa a
//
LOCUS       DAWKOD010000258          660 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000258 DAWKOD010000000
VERSION     DAWKOD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.258"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            227..520
                     /locus_tag="WJK73_002052"
     CDS             227..520
                     /locus_tag="WJK73_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558470.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          168 a          164 c          171 g          157 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tattgtccat
       61 tcggcaggga aagcctgaag aagaattttc ccgcaagatg gccggctgcc taaccgactt
      121 ggaaaactgt ttggaaaaat acctcgaaca gttcgggccg gtatcggaaa gtatcgaagc
      181 ctgtaccgtc aaactgcagg agtaaccgtc gttcttcaac aggctgatga aaaccaacga
      241 caagctcaat aggcagattg acgtgttgca gaaacagtcg gcagccatcc acaacgaagc
      301 ctatatcgaa atgaacacgc tgctttaccg ccaccgcgaa gtggtttccg tccacaaccg
      361 caaggcggat tatgcggaaa agggcaaaga gcggattgcg ctgttcccgc gcggcttgaa
      421 cggcatcacc aagctgcctg ccgccgtcct gttgcccgag cgtcccgatt gttttgatat
      481 gaaggcagtt tggcaaatgt ttaatagaat accgaggtag gcgtatcggc tgccggataa
      541 aaatgccgtc tgaaacggaa gcgttcagac ggcattgttg cagtggaaca aatcaaaatg
      601 caccccgtca ttcctgtctg tacggtgata gcggcagtgt tttcctgctt cgttttatag
//
LOCUS       DAWKOD010000259          652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000259 DAWKOD010000000
VERSION     DAWKOD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.259"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJK73_002053"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJK73_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ7558471.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          116 g          130 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWKOD010000260          634 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000260 DAWKOD010000000
VERSION     DAWKOD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.260"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..359)
                     /locus_tag="WJK73_002054"
     CDS             complement(<1..359)
                     /locus_tag="WJK73_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7558472.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAK"
BASE COUNT          234 a          133 c           75 g          192 t
ORIGIN      
        1 aatttcgcgc ctaagcgggc aagtttgccg acccccgcca atacgccagc gcgggaaact
       61 gatgaagata caaccgaact aactttaccc gtacttgatg ctccagtaga tacatgccta
      121 acttttaaat cttgagcttc tgaagttaaa tatcttcccg attctctatc aaatacacct
      181 ttccaaattc ttcttctttt gtcatctgaa cctcgaatac ctaaatatct gccatcatca
      241 taaacttcaa ccctatcaaa tttacccgga gcatttactg atgccaatgc aaatttagat
      301 acaaaaaaac taaagcatag gataatcttt aaaaaatttc gccctaggat aataaacata
      361 acattttgct tcatctaatt taattaataa tgtagaagaa tcagggaatt gaatttctat
      421 aaaatctcta tcataacgat gaaagaaatt cctaaaaata tcaaatgaaa acgaagtttt
      481 aaaaacccct tttctaatca attccctata ataaattaac gcttgtggta aatctctata
      541 aatatgtgaa tcagtttgaa atttaactaa atgatattca gaaataaaca taattcggac
      601 ctcttaaaat cactctaaaa atccgattct acca
//
LOCUS       DAWKOD010000261          633 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000261 DAWKOD010000000
VERSION     DAWKOD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.261"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..599
                     /locus_tag="WJK73_002055"
     CDS             <1..599
                     /locus_tag="WJK73_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7558473.1"
                     /translation="ASAAVVDATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDSDARW
                     TKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGY
                     DSAENRQHLKEHQLQDGIMRKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFR
                     YARAAYFGLIKVSAQSHLKAMCLNLLKAPNRLSAPVAA"
BASE COUNT          181 a          178 c          163 g          111 t
ORIGIN      
        1 aagcatccgc cgccgtcgtt gacgccacca ttattcagac cgccggcagc aaacagcgcc
       61 aggccataga agttgacgaa gaaggacaag tcagcggcca aaccacaccg agtaaagaca
      121 gcgatgcccg ttggacaaag aaaaacggcc tctacaaact cggttacaaa cagcataccc
      181 gtaccgatgc agaaggctat atcgagaaac tgcacattac ccccgccaat gcccatgagt
      241 gcaaacacct gtcgcctttg ctggaaggga tagccgaagg tacgaccgtc tatgccgaca
      301 aaggctatga cagtgcggaa aaccggcaac atctgaaaga acatcagttg caggacggca
      361 ttatgcgcaa agcctgccgc aaccgtccgc tgtcggaagc gcaaaccaaa cgcaaccggt
      421 atttgtcgaa gacccgttat gtggttgaac aaagcttcgg tacgctgcac cgtaagttcc
      481 gctatgcccg ggcagcctat ttcggactga ttaaagtgag tgcgcaaagc cacctgaagg
      541 cgatgtgttt gaacctgttg aaagcgccca acaggttaag tgcgcctgtt gccgcctaaa
      601 aagcagcccg gatgcctgat tatcgggtat ccg
//
LOCUS       DAWKOD010000262          618 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000262 DAWKOD010000000
VERSION     DAWKOD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.262"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            185..469
                     /locus_tag="WJK73_002056"
     CDS             185..469
                     /locus_tag="WJK73_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7558474.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          181 a          103 c          160 g          174 t
ORIGIN      
        1 tgtcggtgcg gaaacttatc gggtaaaacg gtttctttag attttgcgtt ctagattccc
       61 actttcgcgg gaatgacgaa gagttgcggg aatgatggaa agctatggga ataacgaagg
      121 gttaaagtaa tcacgggatg gtgttcgcgg gaatataaat tgaaataatt caaaagggta
      181 ttatatgcag cctgcggttt atattttagc aagccaacgt aatggcacgt tatacattgg
      241 cgttacatct gatttggtgc aacgtattta ccaacatagg gagcatttga ttgagggatt
      301 tacatcacgg tacaacgtta ctatgctggt ttggtatgaa ctgcatccta cgatggagag
      361 tgcaattact cgggaaaaac agttgaagaa atggaacagg gcttggaaat tgcaactgat
      421 tgaagaaaat aatgtttctt ggcaggattt atggtttgat attatttagc ccgtcattcc
      481 cgcgcaggcg ggaatctaga ccttagaaca acagtaatat tcaaagatta taaaagaccc
      541 gtcattcccg cgcaggcggg aatctaggtc tgtcggcacg gaaacttatc gggtaaacgg
      601 tttcttgaga ttccgcgt
//
LOCUS       DAWKOD010000263          616 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000263 DAWKOD010000000
VERSION     DAWKOD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.263"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..260)
                     /locus_tag="WJK73_002057"
     CDS             complement(<1..260)
                     /locus_tag="WJK73_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558475.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAK"
     gene            complement(244..561)
                     /locus_tag="WJK73_002058"
     CDS             complement(244..561)
                     /locus_tag="WJK73_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7558476.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          181 a          124 c          105 g          206 t
ORIGIN      
        1 aatttcgcgc ctaagcgggc aagtttgccg ccccccgcca atacgccggc gcgggaaatg
       61 ctggcggtta ttttggcgtt gattcgggct tttgcgcccg tagggatgtg tgttaaatct
      121 accgttttta ttaaatcaga tgaataagtt ttactatttt taggtacaaa cttatgaatt
      181 ttcgcacctt gtccggtatc aactgaaaca gtttcagata tttttactgc atttgcattc
      241 gcttcaaacg aatacatcat caaaattgca attatcgaca atttcgcaaa attcaaattt
      301 gtatatttta tgaccatctt tcagggattc tttaattacc atttctgaat tatcagaaaa
      361 tgagattagc caaatatcat gtttaattct tctattccag aaaaaagaga aacaatcaat
      421 aacattttca gacttattaa tcttcgcaaa ttcaacaaat tcagattgcg ctataaccgc
      481 catcgattgc ccaaaatact tgctagacgg ctgatattta taaagtgcca actgcgcctg
      541 agtgataaac ggcttgttca tggttctgcc tttcaatgat tgttttgaaa gcctgatttt
      601 gacaccataa cttcat
//
LOCUS       DAWKOD010000264          569 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000264 DAWKOD010000000
VERSION     DAWKOD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.264"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..>569
                     /locus_tag="WJK73_002059"
     CDS             1..>569
                     /locus_tag="WJK73_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7558477.1"
                     /translation="MHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHKVA
                     LVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFAQ
                     MTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQL
                     IAHFDRLIDELDKQIDNHTHTHFDGKAQVA"
BASE COUNT          174 a          179 c          120 g           96 t
ORIGIN      
        1 attcactata tcgacatcgc caaacgaaac ttcgtcatcg ccgtttcgtc tttgtctaaa
       61 accaaaaccg aaaccaacaa ccccaaaggt atcgcccata ctatcgaata ccttaaaaaa
      121 cacaaggtcg ccctcgtcgt gacggaaagt actggcggtc tcgaaatccc cgccgccaaa
      181 gccatccgcc gagcaggcat agccgtgatt atcgccaacc cgcgtcagac gcatcagttt
      241 gcccaatcgc agccgctgac caaaaccgac gccaaagatg ccaaaatgcc cgccttcttc
      301 gcacagatga cggcacagaa agaagattcg caaaccatgc cctaccaacc gcccaccgaa
      361 gcggaagaag tgttggaagc cttggttaac cgccgcaacc aatcggcgga tatgcggact
      421 gccgagaaaa accgtctgca tcaggttcac gaaacgcaag tcggaagcgt caaacaactg
      481 attgcccatt ttgaccggct gattgacgaa ttggacaaac aaatcgacaa ccacacccac
      541 acgcattttg acggcaaagc ccaagtggc
//
LOCUS       DAWKOD010000265          559 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000265 DAWKOD010000000
VERSION     DAWKOD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.265"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..70)
                     /locus_tag="WJK73_002060"
                     /pseudo
     CDS             complement(1..70)
                     /locus_tag="WJK73_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            360..>559
                     /locus_tag="WJK73_002061"
     CDS             360..>559
                     /locus_tag="WJK73_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558478.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIV"
BASE COUNT          149 a           93 c          172 g          145 t
ORIGIN      
        1 acctgtaaag gaaggggcat cggccgccgt cggtgctttt ttgtttggaa gggaaatggc
       61 tggaaaaaga cctgcgctat atcggggaat gaatcgacgc aaagaaaatg ccgtctgaac
      121 atgcgttcgg gcggcgtttt gttggggggt atcggagcgg aacgtctgaa aaagggtttc
      181 aggcggtctt tgggcgtgtg gtaacagtcg aaaacgtgat aaggctacct gaaaagtttg
      241 ggagattttc aggtagtctt tggtattggg cgcaacagac gcaggtacag attaggcggt
      301 gtgccgtaat cgtacgaatg ccgattcaac ctaagcagac atcagtattt aggaagtgga
      361 tgtttgatgg agcaaaggtt gtacgaaggg tggaaggcaa cctgtgggtg tttggtatgg
      421 tcgcgcttga aaaaacgtgt tttaagggac aaatgccgtc tgaaaatcgg tttcagacgg
      481 cattttctgt ttatttaaag caaacaggaa aaggcagcaa tattctgcag tcttcctatt
      541 cacacaagcg ttttatagt
//
LOCUS       DAWKOD010000266          544 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22438_Neisseria
            meningitidis SAMN10275864-rid8981503.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DAWKOD010000266 DAWKOD010000000
VERSION     DAWKOD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275864
            Sequence Read Archive: SRR8200340
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676327.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676327.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:18:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,062
            CDSs (total)                      :: 2,014
            Genes (coding)                    :: 1,927
            CDSs (with protein)               :: 1,927
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 43 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 9 of 87
            Pseudo Genes (multiple problems)  :: 3 of 87
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275864-rid8981503.denovo.266"
                     /isolate="M22438_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1981"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            58..345
                     /locus_tag="WJK73_002062"
     CDS             58..345
                     /locus_tag="WJK73_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7558479.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          181 a           64 c           92 g          207 t
ORIGIN      
        1 aatggcaaaa caggtaatga attaaagaca ttacctgttt ttacagaaat ttttaaaatg
       61 gatgatttga ttgcagcttt tttaggctgt ttatcaggta aatatagcga agggaaaata
      121 agcagattag aaatatctct aacggcagga agcattttct ttgcagtatt ctttattcat
      181 gaactgataa tttttattgt tgatggaaat aaggaaaatc tttggagttt tattactggg
      241 ataatgaaag atttattttt attttcatgt tctgtttgtt tattattcta cttcttatta
      301 tttattgcgg aaaccattgg gaaaacaatc aataagatta aataagatat ttaagccgct
      361 aaaaaaccat ttgagtattt tatcttctat gcggcattta tcttggaatt gaaatactgc
      421 aatcgggagg ctaaggcttc cgttgtaacg tttaccatat atttcattat tttaatttca
      481 tcaaaaaaag aaattcaaga ttatacatac cgccaatagt ttcttgattg ttttgtatag
      541 tgga
//
